LOCUS       DASRNA010000001         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_970714,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000001 DASRNA010000000
VERSION     DASRNA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970714"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VFD08_00005"
     CDS             complement(<1..725)
                     /locus_tag="VFD08_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK09428.1"
                     /translation="MKTNDLTQGNIYSQLIRLSLPLMGTALIQMSYNLMDMFWLGKLS
                     THSLAASAAVGYISWLAMAFVLMIRTGTEIGVGQSVGAKKTKETQEFITTSLVISVFI
                     GTIYALSAVALRTQLIGLFRFQEEIIRQEAYKFLLIVVLGYPFFFLNPIIGGIYHGAG
                     NSKTPFIVNSVGLMLNMVLDPLFIFTLDMGIQGAALATALANFSVSLIYFLIRKKVGL
                     LANINLIKDFCFDKVKRILSWGYP"
     gene            complement(893..1585)
                     /locus_tag="VFD08_00010"
     CDS             complement(893..1585)
                     /locus_tag="VFD08_00010"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZK09429.1"
                     /translation="MSKTIGIIGGMGPLASVDLQEKIILNTKAASDKEHLHVITDCNT
                     NIPDRTKAILEGGESPLAEMKKSAKRLEKAGAQVLLIACNTAHYYLVQLEKEVNLPFI
                     SMVDAVLKEVEKKEVKKISILSTKGVYKTGLYQKKCQERSIAYLIPEKKDRQLMMDII
                     YGLKAGRLQENTSRMEFFLEAERKKGVELFLLACTELPIYFSYNKIDVPLLDSTLCLA
                     LEAIRYAGGKTR"
     gene            complement(1578..2588)
                     /locus_tag="VFD08_00015"
     CDS             complement(1578..2588)
                     /locus_tag="VFD08_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016741069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZK09430.1"
                     /translation="MGLKSKLGTLPKGKKNSLCDVPGVRVGHKTLNKGKSQTGVTAII
                     PAEGSLFKNKVEAGVSILNGFGKSVGLMQVEELGTLETPIVMTNTLSVGIALSALTKY
                     MLWQNEEIGETTGTVNCLVTECNDSRINDIRGLHVKEEDVLFALNNTDENVEEGAVGA
                     GRGMVCFGLKGGIGSSSRILSFEGHEYSVGALVLSNFGGPGQLRLDGNKIISNSSTGS
                     KGDMGSIIMILATDLPLSSRQLKRLANRCAISLGRTGSIMGHGSGDIALAFSTAQRIA
                     HSPEGIFEKKTYLYEEHIEKVFVAGIEAVEEAIYSSLYHAETMVDRKGKIVHSLKEYW
                     NE"
     gene            complement(2770..>3062)
                     /locus_tag="VFD08_00020"
     CDS             complement(2770..>3062)
                     /locus_tag="VFD08_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase accessory protein HypB"
                     /protein_id="HZK09431.1"
                     /translation="CPAGFDTGATKNAMIISIPEGHDKPLKYPEMFKVVDVVLVNKID
                     AMEYFDFDLDLFRDFVHKINPEIEIIPMSAKTGEGIGPWVDWIRKQVAKQKV"
BASE COUNT          883 a          693 c          518 g          968 t
ORIGIN      
        1 ggataacccc aggataaaat tctttttacc ttatcaaaac aaaaatcctt tataaggttt
       61 atattggcaa gaagtcctac tttttttcgt attaaaaaat aaatcaaaga taccgaaaaa
      121 ttagccagag cagttgctag ggcagcccct tgaattccca tatcaagagt aaaaataaaa
      181 aggggatcca ggaccatatt tagcataagg cccacagaat taacaataaa aggtgtttta
      241 ctatttcctg ccccatggta tataccacca ataatggggt ttagaaagaa aaaaggatag
      301 cctaggacaa caattaaaag aaatttatag gcttcctgcc ggattatttc ttcttgaaag
      361 cggaaaagtc caatcagttg ggttcgaaga gccacagcac ttagagcgta gattgttcca
      421 ataaaaacag aaatcaccaa ggaagtcgta ataaattcct gagtttcctt tgtttttttg
      481 gcaccgacac tctgacccac accaatctcg gtacctgttc ttatcataag aacaaaggcc
      541 atggcaagcc aagaaatata acccacagct gcagaggctg ccaggctgtg ggtgctaagc
      601 tttcctaacc aaaacatgtc cataaggttg taggacatct gaattagcgc tgtccccatc
      661 agtggaaggg aaaggcggat aagttgagaa taaatattgc cttgagtcaa atcgtttgtt
      721 ttcataaggg tatcatacca taaaattatt ctttcaattt ctaggaaaaa tccgtctgct
      781 ttgcttatct tttttaagat aaaactttta cttaagccag acataaacag ggatttttct
      841 agtggtttct attttaagct acctctgaat cagctgcctt tttgctgggc ttttatctag
      901 tcttcccccc agcatatcta atggcttcta gggccaagca cagggtgcta tctagtaggg
      961 gcacatcaat cttgttatag gagaaataaa taggaagttc tgtacaagct agaagaaaaa
     1021 gttctactcc tttttttctc tctgcttcta aaaaaaactc catcctactg gtgttttcct
     1081 gcagccttcc tgcttttagg ccatatataa tatccatcat taactgccta tctttctttt
     1141 caggaataag gtaggcaatc gatctttcct ggcatttttt ttgatagaga cctgtcttat
     1201 atacaccttt agtagaaagg atagaaatct tttttacttc ttttttttct acttccttta
     1261 aaacagcatc aaccatacta ataaaaggaa ggttcacttc tttttcaagt tgcacaaggt
     1321 aatagtgggc cgtattacag gcaatcaaaa gtacttgggc ccctgctttt tctagtcttt
     1381 ttgcgctttt tttcatctca gctagggggc tttctcctcc ttctaaaatg gcctttgtgc
     1441 ggtcaggaat atttgtattg caatcggtta ttacatgcag atgttccttg tcactcgcag
     1501 cttttgtgtt taaaatgatt ttttcttgta aatcaacaga agccaagggg cccattccac
     1561 caattatgcc aatggtttta ctcattccaa tactccttta gactgtgaac tattttgcct
     1621 tttcgatcaa ccatggtctc tgcatgataa agcgaactat aaatagcctc ttctactgct
     1681 tcaattcctg caacaaatac cttttctata tgctcctcat aaagataggt ttttttctca
     1741 aagatccctt ctggactatg ggctattctt tgtgccgtag aaaaggccag ggctatatcc
     1801 ccactaccat gtcccataat tgaaccagtt cttccaagag aaatagcgca gcgatttgcc
     1861 agtcgcttca actgcctgga tgaaagagga agatctgtgg caagaatcat aataattgag
     1921 cccatatcac ccttagagcc agtagaagaa ttagaaatga ttttatttcc atcaagacgc
     1981 agttgacctg gccctccaaa gtttgaaaga accaaggccc caacactata ctcatgtcct
     2041 tcaaaagaaa gaatcctgga ggagcttcct attcccccct taagaccaaa gcagaccatc
     2101 cccctgcctg ctcccaccgc tccttcttct acattttcat cagtattgtt aagggcaaat
     2161 aatacatctt cttccttaac atgtagtccc ctaatgtcat ttatcctact atcattacac
     2221 tcagtaacta gacaattaac cgttcctgtg gtttctccta tttcttcatt ttgccagagc
     2281 atatattttg taagtgcgct aagggctatg ccaacactca aggtatttgt cataacaata
     2341 ggagtctcca aggtgcccag ttcttctact tgcattaggc ccacgctttt accaaaacca
     2401 tttaggatgc ttacacctgc ttctacttta tttttaaata aactgccttc tgctggaata
     2461 attgccgtta ctcccgtttg acttttcccc ttattaagag ttttatgacc tacccttact
     2521 ccaggaacat cgcaaagact attctttttc cccttgggaa gtgttcctaa tttactttta
     2581 agtcccatag cctctccttg tttttcttaa tcatagcacg gaaaacagga aaaatatcac
     2641 tttattctag ctccaagcac aaataggaat agatatttta ccctttctcc ctagatgaaa
     2701 aaagagccct gcacttctag gagttacatc ctaattcgcg cacggccctt tttcattaat
     2761 ggtattttct taaacttttt gcttggctac ctgcttacgg atccaatcaa cccagggtcc
     2821 tataccctcg cctgtttttg ctgacatggg aataatctca atctcaggat tgattttatg
     2881 aacaaagtcc ctaaagaggt ccaaatcaaa atcgaaatat tccatagcgt caattttgtt
     2941 cacaagaacc acatccacca ccttgaacat ctcagggtat tttaggggct tatcatgacc
     3001 ctcagggatg ctaataatca tggcgttttt tgtagctccc gtatcaaagc ctgctgggca
     3061 ga
//
LOCUS       DASRNA010000002         7340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_896072,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000002 DASRNA010000000
VERSION     DASRNA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896072"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VFD08_00025"
     CDS             <1..1415
                     /locus_tag="VFD08_00025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZK09432.1"
                     /translation="TDVLDFNLAPGKEEIAYIATTYEDVREAFKELHLFTLADKKDRI
                     VRKEIKFHYSYFDYLTDKTLIFLGSDGLTHGNNQNHEIYKMDRKSLETRKLSPENWDI
                     SIGSSIAGDTSLIGGRAYKTDGGKFYFSSTEFDHSYLNVIDEEGKIQKLTKLPGSVDT
                     FDVKNGKIYAVLKRDLKLQELYEIKDTEERQITDFNIKGNKLDRAKTESFEFKLNERE
                     YTGYVLLPSDYDPKKKYPGILSIHGGPKAAYGTIFFHELHYFAQEGYIVFFTNPRGSD
                     GRGGDFSDIRGKFGTIDYDDLMDFTDEVLKRYSSIDVDKLGVMGGSYGGFMTNWMIGH
                     THRFAAACSQRSISNWISKFGITDIGYIFNADQHKATPWDRFDSFWEGSPLKHAPNAK
                     TPTLFIHADEDHRCHYSCSMQMHTALKIHGVKSRFVLFHGENHELSRSGKPKERIRRL
                     QEIDNWFAHYLKGEKFEALG"
     gene            1476..2081
                     /gene="lexA"
                     /locus_tag="VFD08_00030"
     CDS             1476..2081
                     /gene="lexA"
                     /locus_tag="VFD08_00030"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZK09433.1"
                     /translation="MALTKKQQDVYDFLKKKILEDGYPPSVREICRGLNFKSTSTAHS
                     YLLKLEKHGYIKRDPTKNRAISLTEDLASSMQNNLSIPLLGRVQAGQPILAVENIEDY
                     FPVSPQFVGSGKHFFLEVSGESMKNAGIFDGDYVLVKQTQDANNGEMVVALLGEETTV
                     KTLYKEKDHIRLQPENDSFEPILTSECEILGIVKAVMRFLR"
     gene            complement(2193..3470)
                     /locus_tag="VFD08_00035"
     CDS             complement(2193..3470)
                     /locus_tag="VFD08_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HZK09434.1"
                     /translation="MYRDFGISHEIQKFVEEQEEALGPLFSALDEVKKTNNLKILKAM
                     QDAGLSDHHFHWNTGYAYDDQGRDATEKIYSQVFDTEDALVRPAIASGTHAITLMLTG
                     ILRPGDHLLTITGTPYDTLLAVIGTSEKNGSTLIELGVEYDEIPMIGKSFDVEKTKNS
                     IKANTKVLYLQRSQGYSFRETFSLKSLEEIISIAKSIKPDIIVALDNCYGEFTNKKEP
                     TSYGVDIMAGSLIKNPGGGLTLSGGYIVGKKDLITLCSHKLTSPSLGKEVGGTFGITR
                     TLLQGLFFAPTVVYHALQSALLFAKVFSELGYDVKPSLEDKREDIIQAIMLKDPNKIL
                     AFCEEVQKSSPVDSHLTPIPWAMPGYDAEVIMAAGTFIQGASIELSADAPMREPYVVY
                     IQGALLYEQAKLCVMRILERFNEMEKRKTGILE"
     gene            complement(3463..3693)
                     /gene="hfq"
                     /locus_tag="VFD08_00040"
     CDS             complement(3463..3693)
                     /gene="hfq"
                     /locus_tag="VFD08_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZK09435.1"
                     /translation="MKKHSLQDRFLFLLKQEEIPVTIFLSSGHHLRGLVQDFDNYTVF
                     IQTGDVEQLIYKHAISSMVPGKHIDFMEEDDV"
     gene            complement(3696..4583)
                     /gene="miaA"
                     /locus_tag="VFD08_00045"
     CDS             complement(3696..4583)
                     /gene="miaA"
                     /locus_tag="VFD08_00045"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZK09436.1"
                     /translation="MRDNLVIFGPTASGKSSLSLELARKNAGEIINMDSMQIYQGMDI
                     ATAKPTKKEREAIAHHLFDIVEPDEDFTTFNYKKLALNCIKEIRRRGNNPILVGGTGL
                     YLSSLYYDFDFRQRNREKRMILEKLFKDKGIEALQEEAEKKYPKLLGEIDKNNPHRLI
                     RLLETGTIQNKKKRSSLALKLIILEEDKDILNEKIRQRIDKMLKDGLLDEVDKVYRLY
                     GDKEYLPAIRGIGYKEYFPYLRGEISLEEAKERHHISTRQYAKRQRTWGRNQYSDAVF
                     IPAHLSLEEKIYKIEEQWR"
     gene            complement(4573..6282)
                     /gene="mutL"
                     /locus_tag="VFD08_00050"
     CDS             complement(4573..6282)
                     /gene="mutL"
                     /locus_tag="VFD08_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZK09437.1"
                     /translation="MQRIHKLSPRVANQIAAGEVITEPLSVIKELLENALDAGASDIR
                     IEIKDSGLKQISIHDNGHGILYEDVDLLFERHCTSKIEVSSDLKNIQSYGFRGEALAS
                     IAAVSKVSLSSRHKDQLQGFETIIYGGRTLSMKRLSRQVGTSFIIEDLFYNTPVREGF
                     LKKPGVLERNIINFLTAMALGNPQISLRFHSDEKLVLETFGRSREKVLIELLGEDARH
                     LIPITMQAEGYRIDGYLSRLSFSKTNRRSQYFLVNNRLVENPELQKLIQKTYEGLLPS
                     RRFPVVFLFIEIDPSKIDVNIHPRKMQVRFSENISLLKDLEDQLRPALYGKQIHPLIE
                     EKQRPPKLSVEEKARTKVAEDIPLSFFTQVDLPVKSDPEEISEAAPAPYDEFFDDLHY
                     LGQAFASFLVFEKATSLYFCDQHAAHEKILYEEFMEEYRKSKLAQQVLMLPQSLSLSA
                     SQLMVLEKKKGFLNDLGFSFDYFSGNDIVLRGVPHLFDTRQAEVFFLDILEGLDQHRD
                     DDQLISQACKAAIKANDRLDRLEVDHLLQRLKTLDNPHTCPHGRPIFIEMPRKELERR
                     FER"
     gene            complement(6266..>7340)
                     /locus_tag="VFD08_00055"
     CDS             complement(6266..>7340)
                     /locus_tag="VFD08_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZK09438.1"
                     /translation="KQTLVGSERFFTEELKELELKILSAEEEQKIYERELYEKLVQKV
                     KTFSQDLLVLSKSLAILDVSLSFSRLARENNYVRPHVDKKRNLELTNSRHPVIEDLQG
                     RENFIANDIYMDDKEHFFIITGPNMAGKSTYLRQIALITLMAHMGSFVPADYALIPLT
                     DRIFTRVGATDDLSQGKSTFMVEMSELSFILHNATEDSLVILDEIGRGTSTYDGLSIA
                     WATTEYLTSEIKPRCLFATHYHELTEAEGKISGIKNFRIAIKQIGSDLVFLRKILPGR
                     SKRSYGIEAAKLAGLPQSLISRASELLLELEKKDNELSPESSLVKETPRISELDHIQM
                     DDLSPREAWSLLEKLKRIYAKNS"
BASE COUNT         2191 a         1411 c         1412 g         2326 t
ORIGIN      
        1 atactgatgt ccttgatttt aatcttgcac caggaaaaga agaaattgct tatatcgcaa
       61 cgacctatga agatgttcga gaggctttta aggaattaca cctttttact ctggctgata
      121 aaaaagatag gattgtaaga aaagaaatta aattccatta ttcctatttt gattatttaa
      181 cagataagac attgattttc cttggaagcg atggtttgac ccatggaaac aatcaaaatc
      241 atgaaatata caaaatggat agaaaaagct tagaaacaag aaagctctcc cctgaaaatt
      301 gggatatttc aataggaagt tctattgcag gcgatactag cctaataggt ggacgcgcat
      361 ataagaccga tggagggaaa ttttatttct ctagcacgga gtttgaccat tcctatctca
      421 atgtaattga tgaagaggga aaaattcaaa agcttaccaa gctgccaggc agtgtcgaca
      481 cttttgatgt aaaaaatggt aaaatttatg ctgttttaaa gcgagaccta aaacttcaag
      541 agctctatga gatcaaagat acagaagaaa gacagattac cgactttaat ataaaaggaa
      601 ataaattgga ccgcgccaaa actgaatcct ttgaattcaa gctaaatgag cgagaatata
      661 caggatatgt acttcttccc tcggattatg atccaaagaa aaaatatcca ggtattcttt
      721 ccattcatgg tgggccaaag gctgcctatg gaaccatatt tttccatgaa ctccactatt
      781 ttgcccaaga aggctatatt gtatttttta caaatcctag gggaagcgat ggacgtggtg
      841 gagactttag tgatattaga ggaaaatttg gaaccattga ctatgatgat ttaatggact
      901 ttactgatga ggtgttaaaa cgttattcca gtattgatgt ggataagctt ggtgtaatgg
      961 gtggtagcta tggaggtttt atgaccaact ggatgatagg tcatactcac cgctttgcag
     1021 ctgcttgttc tcagcgaagc atcagcaact ggattagtaa gtttggtata acagatattg
     1081 gttatatttt caatgccgat caacataagg caacaccatg ggatcgtttt gacagttttt
     1141 gggaaggtag tcccctaaag catgctccca atgcaaaaac acctactctt tttattcatg
     1201 cagatgaaga ccaccgttgt cactatagct gctccatgca gatgcatacg gctctgaaaa
     1261 ttcatggtgt aaaatcacgt tttgttttat tccatggcga gaaccacgag ctttcgcgtt
     1321 ctggcaagcc aaaagagcgt atacgtaggc tccaagaaat tgataattgg tttgctcatt
     1381 accttaaagg ggaaaaattc gaagctcttg gctaacgaac agatgtttga ccctcaagaa
     1441 ggtctatgat acaatgctta caagggaggt ggattatggc tttaactaaa aagcaacagg
     1501 atgtatatga ttttttgaaa aaaaagattc tagaagatgg ctatcctccg tctgtaagag
     1561 aaatatgtag aggtttaaat tttaagagta cttctacagc acacagttat ctcttaaaac
     1621 tagaaaagca tggttatatt aaaagagatc ctactaaaaa ccgagctatt tcactgacag
     1681 aagatcttgc ctcttctatg caaaataacc taagtatccc ccttcttggt cgtgtgcagg
     1741 caggtcaacc cattctagca gtagagaata tcgaagatta ttttcctgta tctccacaat
     1801 ttgtaggaag tggaaaacac ttctttttag aagtaagtgg tgaaagtatg aagaatgctg
     1861 gaatttttga tggtgactat gtactggtta agcaaacaca ggatgcaaat aatggtgaga
     1921 tggtggttgc tcttcttgga gaggaaacta ccgtaaaaac cttatacaag gaaaaagacc
     1981 atataagatt acagccagaa aatgatagtt ttgagcctat tcttaccagt gaatgtgaaa
     2041 ttttaggtat agtaaaggca gttatgcgtt ttctgcgata aaaacaataa aaggtagcag
     2101 ttaattctgc tacctttttt attgcataat tcctaataag tttattactc ctttttttac
     2161 cactagccaa agaaagaatg ggtatatggt tttcactcca aaataccggt ctttcttttt
     2221 tccatttcat taaatcgctc cagaatcctc ataacacaaa gttttgcttg ttcatacaag
     2281 agggcacctt gaatgtatac tacatagggt tctctcatag gagcatcagc gctaagttcg
     2341 atactagcgc cttggataaa ggtgcctgcc gccataataa cttctgcatc ataacctggc
     2401 atagcccaag gaatgggtgt caaatgacta tctacagggg aagatttttg cacctcttcg
     2461 caaaaggcta aaattttgtt ggggtcttta agcataatgg cttgaataat atcttctctt
     2521 ttgtcttcga ggctgggctt aacatcgtaa ccaagctcag aaaagacctt ggcaaaaaga
     2581 agggctgatt gtagagcgtg atataccact gttggagcaa agaataaacc ttgtaaaaga
     2641 gtacgggtaa taccaaaggt cccccctact tcctttccaa gactgggtga agtgagttta
     2701 tgagaacata aggtaatgag gtctttttta ccaacaatat agccaccact taaggtaagt
     2761 ccgcctccag ggtttttaat taatgaacca gccataatat ctaccccata ggaagtgggt
     2821 tcttttttat tggtaaattc accatagcaa ttatcaagag caacaataat atcaggtttg
     2881 atacttttgg cgatagagat aatttcttct aggcttttta gagaaaaggt ttctcgaaag
     2941 gagtatccct gagatcgctg tagatagaga accttggtat tggctttgat ggagtttttt
     3001 gtcttttcca catcaaagga tttgccaatc ataggaatct catcatattc cactcccagc
     3061 tcaattaggg ttgacccatt tttttcgctg gtaccaataa cagctaagag cgtatcatag
     3121 ggagtgcctg taatggtaag aagatgatct cctggtcgaa gtatgcctgt aagcataaga
     3181 gtgatggcat gagttcctga ggcgatagca ggtcgtacta gagcgtcctc agtatcaaag
     3241 acttgagaat aaattttttc agtggcatct ctaccctgat catcataggc atatccagta
     3301 ttccagtgaa aatgatgatc actaagtccc gcatcttgca tagctttaag tatttttaaa
     3361 ttatttgttt ttttaacttc atccaaggca gaaaagagag gccctagagc ctcctcttgt
     3421 tcttcaacga atttttggat ttcgtgagag attccaaaat ctctatacat catcttcctc
     3481 cataaaatca atatgtttac ctgggaccat agagcttata gcatgcttat atataagttg
     3541 ttctacatct ccagtttgta tgaatactgt gtaattatcg aagtcttgaa caagcccacg
     3601 aagatgatgt ccacttgata aaaatattgt aacaggtatt tcttcttgct taagaaggaa
     3661 caaaaaccga tcttgtaaag agtgtttttt cattactacc tccattgttc ttctatttta
     3721 taaatttttt cttcaaggct tagatgagcc gggatgaaaa ccgcatctga atattgattt
     3781 ctcccccagg ttctttgcct tttggcatac tgacgggtac tgatatggtg gcgctctttg
     3841 gcctcttcta aggaaatctc cccccttaaa taagggaagt actctttata acctataccc
     3901 cttattgcag gaagatactc tttatcccca tagaggcggt ataccttatc tacctcatca
     3961 agcaagccgt cttttaacat tttatcaatg cgctgtctaa ttttttcatt tagaatgtcc
     4021 ttgtcttctt ccaggattat aagcttcaaa gcaagagagg atcttttctt tttattttgt
     4081 atggtacctg tttctagaag gcggattagt ctatggggat tgtttttgtc tatttctcca
     4141 agaagtttgg gatacttttt ttctgcctct tcttgtagag cctcaatccc tttgtcttta
     4201 aagagctttt caagaatcat ccttttttcc ctatttcttt gacgaaaatc aaagtcatag
     4261 taaagactag aaagataaag tccagttcct cccacaagaa ttggattgtt accgcgcctt
     4321 cggatttctt taatacaatt cagggcaagt tttttgtaat taaaggtagt gaaatcttca
     4381 tcaggctcta caatatcaaa aagatgatgg gcaatcgcct ctctttcctt tttagtaggt
     4441 ttggcagttg ctatatccat accctgatag atctgcatag agtccatatt gatgatttcc
     4501 cctgcatttt ttctagcaag ctccagggaa agagaacttt ttccactagc agtaggtcca
     4561 aaaattacca gattatctct caaaacgtcg ctccagttct tttcgtggca tttcaataaa
     4621 gatgggcctg ccatggggac atgtatgggg gttgtctaga gtttttagcc tttgcagtaa
     4681 atggtccacc tctagcctat caaggcgatc atttgcctta atagccgcct tacacgcttg
     4741 ggagattagt tgatcatcat ccctatgctg gtctaaacct tctaggatat ctaaaaagaa
     4801 aacctctgct tgtcttgtgt caaaaagatg gggcacacca cgaaggacga tatcatttcc
     4861 tgaaaagtag tcgaaactaa atccaagatc atttaaaaag ccttttttct tttctaaaac
     4921 catcaattga cttgcactta agcttaggct ttgtggtagc ataaggacct gctgggccag
     4981 cttgcttttt ctatattcct ccataaactc ttcatagaga attttttcat gcgcagcatg
     5041 ctgatcacaa aaatagagac tagtagcctt ttcaaagact aaaaaactag caaaggcttg
     5101 gccaagatag tgtaaatcat caaaaaattc atcataagga gctggcgcag cttctgaaat
     5161 ttcttccggg tcgcttttta caggtaaatc cacttgggta aaaaaagata agggaatgtc
     5221 ctcagctact tttgtccttg ctttttcttc cacactaagc tttggcggcc tttgtttttc
     5281 ttctataagc gggtgaattt gttttccata gagggctggt ctaagctggt cttctagatc
     5341 tttaagcagg gaaatatttt cggaaaaacg cacctgcatt tttcgaggat gaatattgac
     5401 atcaatttta gatgggtcaa tctctataaa taagaaaacc acaggaaatc ttctggaagg
     5461 caaaagaccc tcataggttt tttgaattaa cttttgaagt tcagggtttt ctaccaggcg
     5521 attgtttact aaaaaatatt ggctgcgtct attcgtttta gaaaaactaa gtctagaaag
     5581 gtatccatct attctatatc cttctgcttg catagtaata ggaattagat gcctagcatc
     5641 ctctcctagt aattctatta agaccttctc tctgcttctt ccaaaagttt ctaaaactaa
     5701 tttttcatca ctatgaaaac gaaggctgat ctgaggattt cccaaggcca tggcagttag
     5761 gaaattaata atatttcttt caagaacacc aggttttttt aaaaacccct ctcttactgg
     5821 tgtattataa aaaagatctt cgattataaa actagtgcca acttgtctag aaagtcgctt
     5881 catactcaaa gttcttcccc catatataat ggtttcaaaa ccttgaagtt gatctttatg
     5941 gcgacttgaa agactaacct tacttacagc ggcaatacta gctagtgcct caccacgaaa
     6001 gccatagctt tggatgtttt taagatctga gcttacctcg attttggaag tgcaatggcg
     6061 ctcaaaaaga agatccacat cctcatagag aatgccatgc ccattatcgt ggattgagat
     6121 ttgctttagt cctgaatctt taatctcaat acggatatca gaagctcctg catctagggc
     6181 attttctaaa agctctttaa ttacacttag aggttctgtt ataacctcac ctgctgcgat
     6241 ttggttggca acacgggggg ataatttatg aattctttgc atagatcctc tttaactttt
     6301 caagaaggct ccaagcctct ctgggactaa gatcatccat ttggatatga tcaagttcac
     6361 tgatgcgtgg ggtctctttt accagactcg actctggtga aagctcattg tctttttttt
     6421 ctagttcaag aagtagttct gaagcccgac taattaggct ttggggaagt ccggccagtt
     6481 ttgctgcctc aataccatag gatcttttac tacgacccgg taaaattttt cgcaagaaaa
     6541 ccaaatcact gccaatttgc tttattgcaa tacgaaagtt ttttatccca gaaatcttgc
     6601 cctcagcctc tgtaagctca tgataatgtg tggcaaaaag acagcgaggt tttatttcac
     6661 tagtaagata ttcagtagtc gcccaggcaa tacttagacc atcataggtg ctggtccccc
     6721 taccaatttc atcaagaatt actagagagt cttctgtggc attatgcagt ataaagctca
     6781 actcgctcat ttccaccata aaggttgact tgccttggct tagatcatcc gtagccccca
     6841 ctcgcgtaaa tatgcggtct gtaaggggga taagagcata atcagcagga acaaagcttc
     6901 ccatatgggc cattagggta attagggcaa tttggcgcag ataggtactt ttacctgcca
     6961 tattaggacc agttataata aaaaaatgtt ctttgtcatc catgtaaata tcattggcga
     7021 taaagttttc ccggccctgt agatcttcta taacagggtg gcgactattt gtaagctcta
     7081 aattcctttt cttatcgaca tggggtctta catagttatt ctcccgagca aggcgagaaa
     7141 aactcaaaga aacatccaaa attgctagtg attttgataa aacaagcagg tcctgagaaa
     7201 atgtttttac cttttggact agtttttcgt agagctctct ttcataaatc ttttgttctt
     7261 cttctgcact gagaattttt agttcaagtt cttttaattc ttctgtaaaa aatctttcac
     7321 tgcccactag agtttgtttt
//
LOCUS       DASRNA010000003         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_75726,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000003 DASRNA010000000
VERSION     DASRNA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75726"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1526
                     /gene="miaB"
                     /locus_tag="VFD08_00060"
     CDS             204..1526
                     /gene="miaB"
                     /locus_tag="VFD08_00060"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZK09439.1"
                     /translation="MYTQEGNRPYYYIHTFGCQMNDHDSERMAALFELMGYKEGKSEE
                     EASVILLNTCAIREHAEERVYGFIGRLKHLKDDGMILGVCGCMVQQKHVLNKIKKSYP
                     FVDMIFGTHNYANLPEYLLKALEGEKSVEILEDRQSIVEGIPALRSSKVSALVNIMEG
                     CNNFCTYCIVPYTRGREKSRQADDIVAEIKKLVSKGTREVTLLGQNVNSYGKSLGETI
                     DFPDLLEKINEIEGLYRLRFMTSHPKDLSSKLIEAMKLDKVCPSFHLPVQSGSTKVLR
                     AMNRNYSREEYLSKVKALKETVPGIGLTTDIIIGFPGEEEEDVDQTISLIEEVSFDSA
                     YTFIYSKRPGTPAAKLEDKVSKEEKHKRFDRMLRNLNKIVIDKNKSREGQSYEVLIEE
                     ELSAGIYQGRTPQGFLVRFPASSEDLGKLKKVTITKAKKFSLEGELCH"
     gene            1517..>2817
                     /locus_tag="VFD08_00065"
     CDS             1517..>2817
                     /locus_tag="VFD08_00065"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZK09440.1"
                     /translation="MSLNIKNLTPMMQQYMEIKANHPEHILFYRLGDFYEMFYEDAKL
                     ASKALDLVLTSRNAGGGMKAPLCGIPYHSAQGYIAKLIEKGFTIAICEQVEDPKLTKN
                     LVKREVVKIITQGTLVDEDLLNQGENNYLACIISEKDGYGLSYCDLSTFYLRSSFYGF
                     EEEKELIEELKVLSPAEIIVEKGLPFQATKKIDKSKVSKKLLNTFLKVPPEEGALHSL
                     SALFEYILETQFIEPNTTFDYAFINREDFMRLDSTLYAHLELFETLISKEKKGSLLGL
                     LDHCSTPMGSRLLQQWIQKPLQNISSINKRLDLVEFLYQNPELISLLEDNLSSIYDLE
                     RLISKLVFGHIMPKDLRKMTSSLGVLPQIMENLSNLKDRDLLLGLKTFPELWTYLDES
                     LLKEPAATIADGGVISEKNYPKLQEILYLLENGNRLLLELEA"
BASE COUNT          953 a          499 c          545 g          820 t
ORIGIN      
        1 gatgtaagta gggataatat tttcccctta gttttatttt gcatagtaat ttcctttcat
       61 aggtaatcta ccacagccat cttcccttag caagaccagc tatgttatac ttattctcag
      121 gaggtagtac tttgaaaata ctatttaata aagataaaaa aattcaagct gctcttgaat
      181 cagtagcaag aatttcagat aatatataca ctcaagaagg gaatagaccc tactattaca
      241 tccatacctt tggatgtcaa atgaacgatc atgatagcga acgtatggca gcactctttg
      301 aacttatggg ttataaagaa ggaaaatcag aagaagaggc ctctgtcatt cttcttaata
      361 cctgcgccat tcgcgaacac gccgaagaga gggtctatgg ttttattgga agacttaaac
      421 acctaaaaga tgatggtatg attctaggtg tatgtggttg catggtacaa caaaagcatg
      481 tgctaaataa gataaaaaaa agctatccct ttgtggatat gattttcgga acgcataatt
      541 atgccaatct tcccgagtat ctcttaaaag cgctagaagg agaaaaatct gtagaaattc
      601 tagaagatag gcaaagcatt gttgaaggga ttccggccct tcgaagctct aaagtatctg
      661 ctcttgtcaa tattatggag ggttgcaaca atttttgcac ctactgtatt gttccttata
      721 ctaggggaag agaaaaaagc aggcaggcag atgatattgt tgctgaaatc aaaaagttgg
      781 taagcaaggg aaccagagaa gttacgcttc ttggccaaaa tgtaaacagt tatggtaaaa
      841 gtctaggcga aaccatcgac tttcccgatc ttttagaaaa aataaatgag atagaaggac
      901 tttatcgttt gcgctttatg acttctcacc ctaaggatct tagcagcaag cttattgaag
      961 caatgaagct agataaagtt tgcccttctt ttcaccttcc cgttcagtcg ggttctacaa
     1021 aagttctaag ggccatgaac cgaaattata gcagggaaga gtacttgtca aaagtaaaag
     1081 cgctaaaaga aaccgtgccc ggtattggtc ttacaactga tattattatt ggatttccag
     1141 gagaagaaga ggaagatgta gatcaaacta tttctcttat tgaagaagtc tcatttgatt
     1201 ctgcctatac ctttatttac tcaaaacgac cgggaacacc agctgctaaa cttgaagaca
     1261 aggtaagcaa agaagaaaaa cacaaacgtt ttgatcgtat gctccgaaac ttaaataaaa
     1321 ttgtaattga taaaaacaaa tctagggagg ggcaaagcta tgaagttctt attgaagaag
     1381 aactttcagc tggaatatac caaggtagaa ctccccaagg ctttttagtt cgctttccag
     1441 cctctagtga ggacttagga aagttaaaaa aggttacaat taccaaagca aaaaaattct
     1501 ctcttgaagg tgaattatgt cattaaatat taaaaatctc acacccatga tgcaacagta
     1561 tatggaaata aaagcaaacc atcctgaaca tattctgttt tacaggctgg gagacttcta
     1621 tgagatgttt tatgaagatg ctaaactagc cagtaaggct cttgatcttg ttttaacatc
     1681 acgtaatgca ggtggtggca tgaaggcacc cctttgtggg attccctatc atagtgcgca
     1741 aggctatatt gccaaactca ttgaaaaggg atttactatt gcaatatgcg agcaagtcga
     1801 agaccccaaa ctaactaaaa acttagttaa gcgagaggta gttaaaatta ttacccaagg
     1861 aaccttagtg gatgaagacc tactaaacca gggcgaaaac aactatcttg cctgtatcat
     1921 ctcagaaaaa gatggttatg gcttaagcta ttgtgacctt tctacttttt atttgcgttc
     1981 ttctttctat ggattcgaag aggaaaaaga actaatagaa gagctaaagg tactttctcc
     2041 tgcggaaata attgttgaaa aaggtcttcc cttccaagct acaaagaaaa tcgataaaag
     2101 caaagtttca aagaaattat taaatacttt tttgaaagtt cctccagaag aaggagccct
     2161 ccattctcta agtgcacttt ttgaatatat tttagagacc cagtttattg agccaaatac
     2221 tacttttgat tatgctttta ttaatagaga agattttatg aggcttgata gcacccttta
     2281 tgctcatctt gaactctttg aaaccttgat atctaaagaa aaaaaaggaa gtttactagg
     2341 gcttttagat cactgtagca ctcccatggg aagtaggctc ttgcaacaat ggatacaaaa
     2401 gcctctacaa aatatatcct ctatcaacaa aaggctagat ttggtggagt ttttatatca
     2461 aaacccagag cttattagtc tactagaaga caatcttagt agtatctatg atttagagcg
     2521 acttattagt aaactggttt ttggtcatat tatgccaaag gatttacgta aaatgacctc
     2581 ctctttggga gttcttcccc agataatgga gaatctctca aatctaaaag atagggactt
     2641 gcttttgggt ttaaaaacat ttcctgaact ctggacatat cttgatgaaa gcctcctcaa
     2701 ggaaccagca gcgacaattg ctgatggggg agtcatatca gaaaaaaact accccaaact
     2761 tcaggaaatt ctttatcttc ttgaaaatgg aaatcggctt ttattggagc ttgaagc
//
LOCUS       DASRNA010000004         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_224428,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000004 DASRNA010000000
VERSION     DASRNA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224428"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..106)
                     /locus_tag="VFD08_00070"
                     /pseudo
     CDS             complement(1..106)
                     /locus_tag="VFD08_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            120..440
                     /locus_tag="VFD08_00075"
                     /pseudo
     CDS             120..440
                     /locus_tag="VFD08_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            451..1305
                     /locus_tag="VFD08_00080"
     CDS             451..1305
                     /locus_tag="VFD08_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZK09441.1"
                     /translation="MNLDRSYGAVVVRRYQGDWNTILIKHKEGGHWSFPKGHARDGES
                     IDEVIYRKVKDETGLKVEIDPVYSYRSKYILPEGRPKEVFYHLARPKTDQLKTSTEEI
                     ETMKWYTLAQAKEILTYKEDKVIISQLVDDLARATILEKAIDIALNAHAGQLDKSGQA
                     YVLHPLRVMLSLSDETDRVCAVLHDVVEDSDITIGDLNDMGFSREVIDTINLLTRKEY
                     ESYEDYISRVLLSDRACRVKLMDIKDNMTRTPFDKGNPSYEERTARYTRARNRILSTL
                     QDKRGLRD"
     gene            1298..1798
                     /locus_tag="VFD08_00085"
     CDS             1298..1798
                     /locus_tag="VFD08_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09442.1"
                     /translation="MTKMIEVSASIKKSDVRKMLVTAMIFKNPLLIFLLLGVSYGFSF
                     LMWKTQPISFRSHILIWLGMVILILYLVLKKATSQAKSGEAGGESFVGQKQIYRFYGD
                     YFSYKARKNKEFTDIPYSKLAKFSAKGSFLILYLEGNVALALPKRDIGDKLDEILQRL
                     NQSKGA"
BASE COUNT          699 a          370 c          478 g          626 t
ORIGIN      
        1 catgatttcc ccaaagaata aagactcgag gctggtcgct ctctccaaga atttcaagaa
       61 tttttcctgt aaatttcttc caaccaagct gactatgact tccagcctgg gcagaccagg
      121 gtgttttgct tttaaatacg acccttagtg ttcgcagagc ccaggctgga agtcatagtc
      181 agcttggctg gaagaaattt acaggaaaaa ttcttgaaat ccttggagag agcgaccagc
      241 ctcgagtctt tattctttgg ggaaatcatg ctcgtagtag aaagagcaag ataaaaaagg
      301 gttgtcatct tattcttgaa ggccctcatc caagcccctt atcggcctat cgaggctttt
      361 ttggatgtaa tcactttatt aaaagtaatg attttttagc agaaaaagga ataggtaaaa
      421 tttcttggaa gatagactag gagggaaaaa atgaacctag atagatccta tggtgctgta
      481 gtggtaaggc gttaccaggg agactggaat acgattttaa ttaaacacaa ggagggaggg
      541 cactggtcct ttcccaaggg gcatgcgcgt gatggtgagt ccattgatga ggtcatttac
      601 agaaaagtga aggatgaaac aggccttaaa gtagagattg atccagttta tagttatagg
      661 agtaaatata ttctacctga gggaaggccc aaggaggtct tctatcattt ggctcgacca
      721 aaaaccgacc aattaaaaac gtccaccgaa gaaatagaga ccatgaaatg gtatacgcta
      781 gctcaggcca aagaaattct tacttacaaa gaagacaagg tcattataag tcaacttgtt
      841 gatgatcttg caagggcgac tattcttgaa aaagctattg atattgctct taatgcccat
      901 gctggtcagc ttgacaaatc tggacaggcc tatgtgctcc atccactaag agtgatgctt
      961 agtttaagcg atgaaaccga tagggtctgt gcggtacttc atgatgtggt agaagatagt
     1021 gatataacca ttggtgattt aaatgatatg ggcttttctc gagaagtgat tgatacaata
     1081 aatcttctta cgcgaaaaga atatgaaagt tatgaggatt atatttctag ggttcttctt
     1141 agcgacagag catgtcgggt aaaacttatg gatataaaag ataatatgac aaggactccc
     1201 tttgataagg gaaacccctc ctatgaagag cgcacagctc gatatactcg ggccagaaac
     1261 cgaattcttt ctaccctcca agacaaaaga ggtttacgtg actaaaatga ttgaagtatc
     1321 cgctagtata aaaaagagcg atgttagaaa aatgctggtc accgctatga tatttaaaaa
     1381 ccccctattg atttttttac tacttggggt aagctatggc ttttcttttc ttatgtggaa
     1441 gacccagccc atcagtttta gaagccatat attgatatgg ctagggatgg ttattttgat
     1501 tttatatctt gtattgaaaa aagctacttc ccaggctaaa agcggtgaag ctgggggaga
     1561 aagctttgtg ggacaaaaac aaatttatcg tttctatgga gattattttt cttataaagc
     1621 cagaaaaaac aaggaattta cagatattcc ttatagcaaa ctggctaaat ttagtgccaa
     1681 aggaagcttt ctcattctat atttagaggg caatgtagct cttgctttac ccaaaagaga
     1741 tataggtgat aagctagatg aaattttaca gcgtctaaat caaagtaagg gggcctagca
     1801 gattaaaaat acctgctagg tctttttact atcatggagc catcggtata gagataacaa
     1861 gtaggccttg tgggcttagg ctaaaagatg ctctggggtg tctcaaaact tctgtgaatc
     1921 atgtttattt gagtataggc aaaagaaaaa aggacctata aacttactgg actaatggct
     1981 ctgtgtgtgg gatactaata aaaacactaa aaagtcttta tctctaacta aaagagcaac
     2041 aactaccatg aagattaaga caaattattt atttttaaga gaaaaagcca agcatggctc
     2101 ttaagaagtg gctggttttt gtggtcctag gatcaggatc cttagcttat cgtcaagtgt
     2161 tttttcactt tta
//
LOCUS       DASRNA010000005        10254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_896555,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000005 DASRNA010000000
VERSION     DASRNA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896555"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /gene="upp"
                     /locus_tag="VFD08_00090"
     CDS             <1..275
                     /gene="upp"
                     /locus_tag="VFD08_00090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZK09443.1"
                     /translation="SDIAESEVILLDPMLATGGSGVAAVNFLKEREVKKIKFVCIIAS
                     EEGVEVLHKAHPDVAIFCASVDPILNEDSYIVPGLGDAGDRLFGTK"
     gene            complement(404..685)
                     /locus_tag="VFD08_00095"
     CDS             complement(404..685)
                     /locus_tag="VFD08_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09444.1"
                     /translation="MDKYIKLRKIILRLAIALLMIDLLLTFIQSEYPSSLFYIILFAH
                     AASHLVTQYTRYKAKDESFSKLVFVLYIIFTGFSLYNIVANAIKFISHL"
     gene            1076..3484
                     /gene="leuS"
                     /locus_tag="VFD08_00100"
     CDS             1076..3484
                     /gene="leuS"
                     /locus_tag="VFD08_00100"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZK09445.1"
                     /translation="MRHYNFTEIEKKWQKVWEDEGTFEVKERPGLAKDYILIEFPYPS
                     AQGLHVGHPRSYTALDIVARKRRREGRNVLFPIGFDSFGLPTENYAIKTKVHPKKVTE
                     ENIQHFTRQLKSIGFSFDWSRVVTTSEPEYYKWTQWIFLQLFKEGLAYKTRMDINFCP
                     SCKVGLANEEVVGGECERCGAAVVRREKEQWMLKITKYAQRLIDDLGDLDFLDRIKSQ
                     QINWIGRSQGANILFPIKGIEEELEVFTTRPDTIYGATYMVIAPEHPLIDQYQDRLDN
                     YDALIDYRHKSKHKSDFERTQLVKEKTGIRLEGMRAINPITEEEIPIYISDYVMMEYG
                     SGAIMAVPAHDQRDYEFAQAFGEKIVQTISGASIEEEAYTGEGEVINSPLIDGLSVDE
                     AIKKMITIIEDRGLGEGKVQYKLRDWIFSRQRYWGEPIPVVHCKKCGEVALPEEELPL
                     ILPDVESYETTEDGNSPLAAISEWVNTTCPACGGAAKRETDTMPQWAGSSWYFLRYLD
                     PHNDKALASDEAINYWMPVDWYNGGMEHTTLHLLYSRFWYKFLYDIGLVPTSEPYSKR
                     TSHGFILGENGEKMSKSRGNVVNPDEVINNYGADTLRIYEMFIGDFEKTVSWSENGVA
                     GAKRFLDRVWNLQSMVKNQSKDYSKDLELIIHQSIEKVGSDYERLKGNTAVAQLMSLT
                     NAYYQKGSINRGELEVLLYLLNPLAPHLSEEIYENLGNSKRITDTSWPSFDPEKTIED
                     DVEVPIQVNGKVRFKMVLSRGMERAEVEEQLKANPEYEKYTQGKKVLKIIVVPDKLIS
                     LVIK"
     gene            3494..4864
                     /locus_tag="VFD08_00105"
     CDS             3494..4864
                     /locus_tag="VFD08_00105"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZK09446.1"
                     /translation="MNLSYERSFVWSKKNHKEIFSFAEGYKEFLSFAKTERLTHKRLL
                     SLAKEKGFRSFEELSKDKALLPGAKLYFENKNKSLVLMILGEDLEEGMKIVGSHIDVP
                     RLDLKQVPLYEEGKMAFLKTHYYGGVKKYQWTCIPLALHGLAYTKEGKAVEISLGEAE
                     EDPVFYINDLLIHLSADQMKKKLSEAITGEQLNLVVGHLPLEGEEKDAIKLGVLTLLN
                     EKYGLIEEDFIVSEIEVVPAGKARDVGFDRSMIAGHGHDDRSCAYASLRAILEIQSPK
                     TTAVCIFADKEEIGSVGNSSMEALFFENLISEILAAQKKDPSLARRRALAASKVLSAD
                     VSACFDPNFPEVSEKLNTAFLGCGGNLSKYTGVRGKGGSNDANAEFLQEIRELFDSKD
                     IIWQTGELGKIDQGGGGTIAFILAKYGAEVVDFGIPVLSMHAPIELISKADLYATYEA
                     YRAFME"
     gene            4876..5253
                     /locus_tag="VFD08_00110"
     CDS             4876..5253
                     /locus_tag="VFD08_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZK09447.1"
                     /translation="MKAYHTDKAPAAVGPYVQALKAGNFLYLSGQTPIVPETGKLIEG
                     GIEEQTRQVFKNIEAILEEAGYKKTDVLKSTVFLTDMANFQAMNKVYEEFYGDHKPTR
                     SAFAVKALPVNAIVEIETICYKE"
     gene            5348..6103
                     /locus_tag="VFD08_00115"
     CDS             5348..6103
                     /locus_tag="VFD08_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HZK09448.1"
                     /translation="MNQTIATQKDHRTIRAFKEGKLSEEIIKELLEVARRTATSTGLQ
                     SASIIRVNDQKKRDEIAKVCDQPYVASSSELWIFIVDTYRNNQIAKQKGVDSDAGRDM
                     DRFFQGFTDGSLMAQNVLNAIESLDLGGVYLGSIHNDDRKIIEILELPELTFPIVGLA
                     FGKKDQAPQIKPKMPMLLRCFEDVYKKQEDYLEAFKDYDEVMQTYYDLRDANNRVDSF
                     TNQIATQYSNPSAKRREIIDAIFEQGYTLNPRP"
     gene            6393..7493
                     /locus_tag="VFD08_00120"
     CDS             6393..7493
                     /locus_tag="VFD08_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09449.1"
                     /translation="MKKLRVFMAVLLISTSLLSCAIKQENKGMVSDEKENVSSDIKRE
                     ENNEKTEVFAVLELEYGSQAKGEYEIEKKGEKTPIAISEIDTMKIGKRDYLVKTREDI
                     ELLIAVDIVDTQKPLIIGKDEIEIKIEDLKTFDLRDKIRAVDPVDGQVELDFEMPKIT
                     KAGEYSLEVGAKDSNANQTSKTIKLIVLAEEEYSYDGQTSIEVEGRKFLLEVKPDGPA
                     QETVIKDFLYSITAEFEERSKIYADTEDFVWFLDIEKDEFAFGVYIESYRIHELMTLS
                     EIEYSIDRGEIYGEDPIYYEGWDQIVEEYQLQEYEIITVHFTQEYSQKASEFPPQWGD
                     GTYYKSYLVGKTSTDSNYRIYDMGKLHESAGI"
     gene            7560..7889
                     /locus_tag="VFD08_00125"
     CDS             7560..7889
                     /locus_tag="VFD08_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HZK09450.1"
                     /translation="MARFDDFLKLDIRVGEIVKAENFEKARKPSYKLWVDFGSEIGIK
                     KASAQITELYSLNELLHRQVLGVVNFPPRQVADFMSEVLVLGIYSEEGVILIRPERPA
                     KKGDKLG"
     gene            8159..8647
                     /locus_tag="VFD08_00130"
     CDS             8159..8647
                     /locus_tag="VFD08_00130"
                     /inference="COORDINATES: protein motif:HMM:NF020450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HZK09451.1"
                     /translation="MLPKWEDLPRGPMTNRQLVDYVRGFLTPLFTSENFITSTRIQNY
                     VKWGVLSQPDGRRYNRVHLAEAIIVSILKGVLTTQEIAQGISLQLLLLSTEEAYNAFS
                     RAFEEAKAEVEYFISYGETLENKSKHPALTGVCRAFCYRALSKDIIRLGGLEGFKGEK
                     SG"
     gene            8640..9485
                     /locus_tag="VFD08_00135"
     CDS             8640..9485
                     /locus_tag="VFD08_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK09452.1"
                     /translation="MDKIALVLDTASNMDFEMAEKFGFELLPYSIEIEGDAYDDLIEI
                     PREGFYERLEKEKMSTGVPPLGKIQNFMEKLVEKGYKQALIFTVSPEMSGMYSAMRLH
                     SSVEGLETYVLDTRTVGPGVSLLALYAKDLLQKGYNFNQLIKACTLKRDQLDVAATFR
                     TLVYLIRGGRISSFKGHLGEVLKFFPLISVKDGKLQQIGKARGKVKSQKQLLKKVKAD
                     LAESKDFDMAFFHAENPEEMKIIKEELSDEISRARRLIDFPSTSVLGVHGGPGYFGVS
                     YFIRQ"
     gene            9635..10135
                     /locus_tag="VFD08_00140"
     CDS             9635..10135
                     /locus_tag="VFD08_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HZK09453.1"
                     /translation="MKKTGIIVSLLLLFILGFWFKLYGLTPLEVPQRQIFVHIEGEVT
                     KPGLYEIAEDLRLGDLVELAGGLTDKAEGINLAKKLIDGEKIIISRIWQEDAEDNQVQ
                     NSPSIIREMTLEEWMEIPGVGEVTARAIMEYLRKNPGAKVDELIEVNGIGEKKLQILK
                     EHLGNL"
BASE COUNT         3474 a         1696 c         2261 g         2823 t
ORIGIN      
        1 cgtcagatat tgctgagagt gaagtaatac ttcttgatcc tatgttagca acaggaggaa
       61 gtggcgttgc cgctgttaac ttcttaaagg agcgagaggt taagaaaatc aaatttgtct
      121 gtattattgc ctcagaagaa ggggtagagg ttctacataa agcccacccc gatgttgcaa
      181 tcttttgtgc gtcggtagac cctattctta atgaagattc ttatattgtt ccaggacttg
      241 gtgatgcagg agatcgcctc tttgggacca aataaaaacg gtgtgcccag gcacaccatt
      301 tttatacttt agatcaaatg tatttgattg tactaaaggc aaaactcgta gaatcaacca
      361 tcctatcagt taaaagccct ataataaggg tctttcttat aaatcagaga tgagatataa
      421 atttaattgc atttgcaact atattatata gtgaaaaacc tgtaaatata atataaagaa
      481 caaaaacgag tttagaaaaa ctctcatctt ttgctttgta tcttgtatat tgagtgacta
      541 ggtgactagc tgcatgagca aaaagaataa tatagaataa acttgatgga tattcgcttt
      601 gtataaaggt caagagaaga tctatcatta ataaagcgat tgctagtcta aggattatct
      661 ttcttaattt gatgtattta tccatttttt ctcccgacta cttagtgagt tcacatttaa
      721 atatgattgt aacacatatt ggtaagactt tttaggcaat tttcactatc tgagaagacg
      781 gggaaacaaa aaaacttttt cccccaaaaa acagggccgc tgcatcctta ctttaaataa
      841 atttcttgca aaagcaagga aaattttgta tacttgcact agttaataga gctggaatag
      901 tagaaaataa aacccttaag agagcagccg gctggtggaa ggttgtggga aatatttttg
      961 aactcgccta aggatggagc ttgtcttcct taaaagacac aaagtcgact tggtaaactg
     1021 aggtggttgc gcctataagc gccctcagaa caagtctttt tttgaaagga gcaccatgag
     1081 acattataac tttacagaaa tcgaaaaaaa atggcagaaa gtttgggaag atgagggaac
     1141 ctttgaagta aaggagcgcc ctggtcttgc caaggattat attctaattg aatttcccta
     1201 tccttctgcc cagggccttc atgtaggtca cccaagaagc tatacagctt tggatattgt
     1261 ggctagaaaa agaagaagag aaggcagaaa tgttcttttt ccaattggat ttgatagttt
     1321 tggtcttcca acagaaaact atgccataaa aacgaaagtt catccaaaaa aagtaacaga
     1381 agaaaacatc caacatttta ctcgtcagct aaaaagtata ggctttagtt ttgactggtc
     1441 tagggtagta actaccagtg aaccagaata ctataaatgg acccaatgga ttttccttca
     1501 actatttaaa gaaggtctag cctataaaac aagaatggat atcaactttt gcccttcttg
     1561 caaggttggt ctggccaatg aagaagtcgt aggaggagaa tgcgaacgtt gtggcgctgc
     1621 tgttgttcga agagaaaaag aacagtggat gttaaaaatc acaaaatatg ctcaaagact
     1681 catagatgat cttggggacc ttgatttttt agaccgaata aaatctcaac agataaactg
     1741 gattggtaga agtcagggag cgaatatttt gtttcctata aagggaatcg aggaagaact
     1801 agaagttttc accactcgtc ccgacaccat ctatggtgct acttatatgg ttatcgctcc
     1861 agaacacccc ttgattgatc agtatcaaga caggctggat aattatgatg cccttataga
     1921 ctatcgccat aagtcaaagc ataagagcga ttttgagagg acccagctag taaaggaaaa
     1981 aactggaatt cgcctggagg gaatgagggc gattaatcca atcactgaag aggaaatccc
     2041 aatctatatt tcagattatg tcatgatgga atatggaagt ggagccatta tggccgtgcc
     2101 tgctcatgac caaagagact atgaatttgc ccaggccttt ggtgaaaaaa tagtccaaac
     2161 aatcagtggg gcttctattg aagaagaagc ctatactgga gagggagaag tcatcaattc
     2221 accactgatt gatggactta gtgtagatga agcaatcaaa aaaatgataa ccatcatcga
     2281 agataggggc cttggagaag gaaaagttca atacaaactc cgtgactgga ttttttcacg
     2341 ccagcgctac tggggcgagc caattcctgt ggttcactgt aaaaaatgcg gtgaggttgc
     2401 cttgccagaa gaagagcttc cacttattct tcctgatgta gaaagttacg agacaacaga
     2461 ggatggaaac tctcctctag cagctattag cgagtgggta aataccactt gtccagcttg
     2521 tggaggggct gctaaacgtg agacggatac catgccccaa tgggcaggaa gcagttggta
     2581 ttttttacga tacctagatc cccataatga taaggccctt gctagtgatg aggcaattaa
     2641 ctactggatg ccagtagatt ggtataatgg agggatggaa cacaccactc ttcaccttct
     2701 ttatagtcgt ttctggtata aattccttta tgatattggc cttgttccca catcagagcc
     2761 ttactctaag agaacaagcc atggctttat ccttggtgaa aatggggaaa aaatgagtaa
     2821 atcgcggggg aatgtagtaa accccgatga agtgattaat aattatggtg ctgatactct
     2881 caggatctat gagatgttta taggtgactt tgagaaaaca gtttcttggt cagaaaatgg
     2941 cgtagctgga gccaaacgtt tcctagatag ggtatggaac ctacagtcca tggtaaaaaa
     3001 tcagagcaaa gactatagta aagaccttga gctgattatt catcagagta tagaaaaagt
     3061 tggcagtgat tatgagagac ttaagggcaa tacagctgtt gctcagctta tgagcctaac
     3121 caatgcctat taccaaaaag gaagtataaa tagaggtgaa cttgaagttc tactctatct
     3181 tctaaatcct cttgctcccc atctaagtga ggaaatatat gaaaacttag gcaatagcaa
     3241 acgcataaca gatacaagct ggccaagctt tgatccagaa aaaaccatag aagacgacgt
     3301 cgaagtgcca attcaggtca atggcaaggt gagattcaaa atggttttat ctaggggaat
     3361 ggagcgcgct gaggtagaag aacaacttaa agcaaatcca gaatatgaga aatatactca
     3421 aggaaaaaag gttcttaaaa ttatagttgt accagataaa ctgattagtc ttgtaataaa
     3481 gtagaggtaa aaaatgaatc taagctatga acgctctttt gtctggagca agaaaaacca
     3541 taaggaaata ttttcttttg ctgagggcta taaggagttt ttatcctttg ctaagactga
     3601 acgcctgact cataaaagac ttttaagttt agccaaagaa aagggcttta gaagctttga
     3661 agaactaagt aaggacaagg cccttcttcc aggcgccaag ctgtattttg aaaataaaaa
     3721 caaatccctt gtcctaatga ttttaggaga agatttagaa gaaggaatga aaattgtcgg
     3781 aagccatatc gacgttcctc gactcgattt aaaacaagtc cccttatatg aagagggcaa
     3841 gatggcattt ttaaaaactc attactatgg aggagttaag aaataccagt ggacctgcat
     3901 ccccctggct cttcatggat tagcctacac caaagaggga aaagctgtag aaatctccct
     3961 aggagaagcg gaggaggacc cagtctttta catcaatgac ctattgattc atctatctgc
     4021 tgatcagatg aaaaagaaac tatccgaagc aattacagga gagcagctca atctagttgt
     4081 aggtcatctg cccctagagg gagaagaaaa agatgccatc aaattaggcg tcctaacact
     4141 tctcaatgaa aagtatggac taattgaaga ggactttatt gtaagtgaaa ttgaagttgt
     4201 cccagcgggt aaggcaaggg acgtaggatt tgatcgttct atgattgcag gacatggtca
     4261 tgatgatagg tcctgcgcct atgcttcgct aagggcaatc ttggaaatcc aaagtcctaa
     4321 aaccactgcc gtatgtatat ttgctgataa agaagaaatt ggttccgtag gcaatagttc
     4381 tatggaagca ctattttttg aaaatttaat tagtgagatt cttgcagctc agaaaaaaga
     4441 cccttctttg gctagaagaa gagccctagc agcaagcaag gtgctttctg ccgatgtgtc
     4501 tgcttgtttt gatccaaact tcccagaagt gagtgaaaag ctaaataccg catttcttgg
     4561 ttgtgggggg aatctaagca aatataccgg tgttcgaggc aagggtggta gcaatgacgc
     4621 caatgctgag tttctacagg aaataagaga actttttgat agcaaagata ttatttggca
     4681 aacaggagaa ctaggaaaaa tagaccaagg tggaggtgga acaattgcct ttatccttgc
     4741 aaaatacggg gctgaagtag ttgactttgg cattcctgtt cttagtatgc acgcgcccat
     4801 agaattaatt agcaaggcgg atctttatgc aacctatgag gcatataggg cctttatgga
     4861 ataaaaggag gaattatgaa agcatatcac acagacaagg caccagcggc cgttggaccc
     4921 tatgttcagg ctctaaaagc aggaaacttc ttgtatcttt ccggccagac cccaattgtt
     4981 cctgaaacag gaaaactaat tgaaggcgga attgaagagc aaacacgtca ggtttttaag
     5041 aatattgaag ctattttaga agaggcaggt tataaaaaga cagatgtgct taaatccacg
     5101 gtatttctga cagatatggc aaactttcag gccatgaata aggtatatga agagttttat
     5161 ggggatcata agcctacaag aagtgccttt gccgtaaaag cccttccagt aaatgctatt
     5221 gttgagattg agaccatttg ttataaggag tagtagggag gaaaaaagag ttttctaagg
     5281 ggaactcttt tttcttcttt ttaatttatt gataaagggg tatagtcaaa gtaataagga
     5341 ggtaaatatg aatcaaacca ttgccacaca aaaggaccat agaactatca gggcctttaa
     5401 agagggaaaa ctcagtgaag agataataaa agaactccta gaagtagccc gaagaactgc
     5461 taccagtaca ggtttacaaa gcgcttccat tattcgtgtc aatgatcaaa aaaagagaga
     5521 tgagatagcc aaggtatgtg accagcccta tgttgctagt tcatcagaac tttggatctt
     5581 tattgtagac acctatagaa acaatcaaat tgccaagcaa aagggagtag atagtgatgc
     5641 tggcagagat atggaccgtt tctttcaagg atttacagat ggaagcctta tggctcaaaa
     5701 cgtccttaac gcgatagaaa gtctggatct aggcggagta tatctaggaa gcattcacaa
     5761 tgatgatcgc aaaattattg aaatcctaga actcccagaa ctgacctttc ccattgtagg
     5821 tcttgctttt ggaaaaaagg accaggcacc tcaaatcaag ccaaaaatgc ccatgttact
     5881 tcgatgtttt gaagatgtct ataaaaagca ggaagattat ctagaggcct ttaaggacta
     5941 tgatgaggta atgcaaacct attatgattt aagagatgct aataatagag tggattcctt
     6001 tacgaaccaa atcgccacac aatatagtaa tccgagcgca aaaagaagag aaattataga
     6061 tgctattttt gagcaaggtt atactctaaa tccccgaccc taggggattt ttttcttgtg
     6121 attaaatact tttaaagaaa aagcaaagta aaataagtaa aaaagccaaa gcttagatga
     6181 caaggccaaa taccgtgtta tactataatt actaataaat tgatataatc ctatacttgc
     6241 ttatattgtt tttttagaga agttgggtac atatcgattt taactttttg aagctcgata
     6301 aagcagggtt tttctttagg cctaaagggc agagcttggg tgatagcaaa atcatagata
     6361 gtttgcaaat cgttaaaaaa ggggagcgca agatgaaaaa gctaagagtc tttatggccg
     6421 ttttgttaat ttcaaccagt ctactttcct gtgccattaa gcaagaaaac aaagggatgg
     6481 ttagtgatga aaaagaaaat gtttcaagtg atatcaagag ggaagaaaac aatgaaaaaa
     6541 cagaagtatt tgcggtttta gagttagaat atggctccca agcaaagggg gagtatgaaa
     6601 tcgaaaaaaa aggcgaaaaa actcccatag ctatctctga aattgacaca atgaaaatag
     6661 gcaaaaggga ttatctggta aaaactagag aagatataga gctacttata gcagttgaca
     6721 tcgtagatac acaaaaacct ctaattatag gcaaggatga aattgaaata aaaatcgaag
     6781 atcttaagac ttttgactta agggataaga taagagctgt agatcccgtt gatggacaag
     6841 tcgagttaga ttttgaaatg ccaaaaataa caaaagcagg tgaatactct cttgaggtag
     6901 gagcaaaaga ctctaacgca aaccaaacaa gtaaaacaat taaacttatt gtattagcag
     6961 aagaagagta tagctatgat gggcagacca gtattgaagt agaaggacga aagtttctgt
     7021 tagaagtaaa accagatggc cctgcacaag agacggtgat aaaggatttc ttatattcta
     7081 ttacagctga gtttgaagaa agatcaaaaa tttatgctga tacagaagat tttgtctggt
     7141 ttttagatat tgaaaaagat gaatttgcct ttggtgtcta tattgagtcc tacaggattc
     7201 atgaactaat gactttatca gaaatcgaat atagtattga tagaggggaa atatacgggg
     7261 aagatcccat atattatgaa ggatgggacc agatagtaga ggaataccaa ttgcaggaat
     7321 atgaaattat aacggttcat tttacccaag agtattcaca aaaagcaagt gaatttcccc
     7381 ctcagtgggg agatggcact tactacaaaa gttatctagt gggtaagaca tctacagatt
     7441 caaattatag gatatatgat atgggaaaat tacacgaaag cgcaggaata taattttttt
     7501 tgctaatgca aattcactaa gaaaagataa gtactattag ataagcagga ggccctagta
     7561 tggcaagatt tgatgacttc ttaaaactag atattcgagt gggagagatt gttaaagcgg
     7621 aaaactttga aaaagcaaga aagccttcct ataaattatg ggttgatttt ggtagtgaaa
     7681 tcgggattaa aaaagccagt gctcaaatta ctgaactata tagtttgaat gaattactgc
     7741 atagacaggt tttgggggtg gtaaattttc ctcccaggca agttgcagat tttatgtctg
     7801 aagtattggt gctgggaata tactcagaag aaggcgtaat attaattcga ccagaacgcc
     7861 cagcaaaaaa gggagataaa ctaggttaga atagtttcct ttgtcgtaag aaaaaaggca
     7921 aggacaatcc tttcttacac tagctttatg actggctcta gtatttggac atctgtggag
     7981 cttgccctaa gtggttttta cttggaataa atagaaactt cctctttaaa agtgagagca
     8041 ataagtttat aagcggccaa tcaacaagtg tttctaggaa gacgcttgtt tttttcttga
     8101 cataggcaaa aaccgtgata agatgtatct agttatcata actagataag gaggaaccat
     8161 gcttccaaag tgggaagatt taccaagggg acctatgaca aatcggcagt tggttgatta
     8221 tgtgcgaggg tttttaaccc cgctttttac tagtgaaaac tttataacta gcactaggat
     8281 acaaaactat gtaaagtggg gagtgttaag ccagcctgat ggaagacgct ataatcgagt
     8341 ccacttggct gaagcaatta tagtctctat tcttaaggga gttcttacaa cgcaggaaat
     8401 tgctcaggga atcagcctcc aacttctctt gctttctaca gaggaggcct acaatgcttt
     8461 ttcaagggcc tttgaagagg caaaagcaga agtagagtac tttatatcct atggagaaac
     8521 cctggaaaac aagtccaaac acccagccct tacaggggtc tgccgtgcat tttgttacag
     8581 agcccttagt aaggatatta tccgattagg aggcttagaa ggctttaaag gagaaaaaag
     8641 tggataaaat agccctagta ttagatacag caagtaatat ggattttgaa atggccgaaa
     8701 agtttggttt tgaactttta ccttatagta ttgaaattga gggagacgcc tacgatgatt
     8761 tgattgaaat ccccagggag ggcttttatg aacgccttga aaaagaaaag atgtctacag
     8821 gtgtcccgcc tctgggtaaa atacagaact ttatggaaaa attagtagaa aaaggttata
     8881 agcaggccct tatttttact gtttctccag agatgagcgg catgtactca gctatgcgcc
     8941 ttcactcatc tgttgagggc ctagagactt atgttcttga tactcgtaca gtagggccag
     9001 gtgtaagcct gcttgctctt tatgccaaag atcttttgca aaagggctat aactttaatc
     9061 aactgattaa agcatgcacc cttaaacgcg atcaactcga tgtggctgcc acctttagaa
     9121 ccctggtcta tttaattaga ggaggtagaa tttcatcttt taagggccac ttaggcgagg
     9181 ttcttaagtt ttttcctctt atttcggtaa aggatggaaa actccagcaa attggaaagg
     9241 ctagaggaaa ggtgaaaagt caaaagcaac tccttaaaaa ggtaaaagct gatttggcag
     9301 aatcaaagga ttttgatatg gcatttttcc atgcagagaa tccagaagaa atgaaaataa
     9361 taaaagaaga actaagtgat gagatttcta gagcaaggcg tcttattgac ttcccctcta
     9421 cctcagtact aggagttcat ggtggtcctg gctattttgg ggtatcttac tttatacgcc
     9481 agtaggcata tcattaagaa ggtaagctag acttttcttc ttgcgataaa tatttgtttt
     9541 ttgtcttaca ttctaatact caaccaataa ggcaattgga tttgattgca gtttttttgt
     9601 attttgccgt gctaatctag acaggaggct aaatatgaaa aaaactggaa tcattgtatc
     9661 tttattgctt ttatttatac tgggtttttg gtttaaactc tatggactta ctcccctaga
     9721 ggttccccaa agacaaattt ttgttcatat tgagggtgaa gttacaaagc ccggtcttta
     9781 tgaaatagcg gaagatttgc gtttggggga tttagttgaa cttgctggag gccttacaga
     9841 taaggcagaa ggtattaatc tggcaaaaaa actgatagat ggtgaaaaga ttattatttc
     9901 ccggatctgg caggaagacg ctgaggacaa tcaagttcaa aatagccctt ctattataag
     9961 ggagatgaca ctagaagaat ggatggagat tccaggagtg ggagaggtca cagcaagggc
    10021 aatcatggaa tatctaagaa agaatccagg agctaaggtg gatgaactta ttgaagtcaa
    10081 tggaataggt gaaaagaaac tccaaatcct gaaagaacat ttgggtaatc tataaaatta
    10141 tgttaaaata tctctgaaag tgaggcataa atggaaaaaa taaaattaac ccagcatgca
    10201 tcagctgcag gctgagcggc taaacttgcc cctgatagtt tatcagatat atta
//
LOCUS       DASRNA010000006         6451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_373946,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000006 DASRNA010000000
VERSION     DASRNA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373946"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFD08_00145"
     CDS             <1..389
                     /locus_tag="VFD08_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HZK09454.1"
                     /translation="NIDYDSIPQLMTQMQEHQINWSMPKIHIKDEIELNQVLRSLGVN
                     QIFEEDGQLGNISEENLQVSLIKQWTDLLVEEKGVEAAAATLVEIKATSLPPELPEVD
                     MKLDRPFSLFIVFRDVPLFFGDVYQP"
     gene            complement(461..646)
                     /locus_tag="VFD08_00150"
     CDS             complement(461..646)
                     /locus_tag="VFD08_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HZK09455.1"
                     /translation="MAKPKKQIPLRLSHKLYEQIAAWAEDDFRSINGQIEYLLTQCVK
                     QRKKDGKYLPEDLSSKE"
     gene            complement(649..1674)
                     /locus_tag="VFD08_00155"
     CDS             complement(649..1674)
                     /locus_tag="VFD08_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZK09456.1"
                     /translation="MQEKILKPKKGMPILILLGLLYIGAILALIFGIRLMNDGSSLGS
                     LLLAGGIFWLLVGIVPFFGLKIIKPKEAIVLTLFGNYTGSIKEPGFYYVNPFSTAVNP
                     AAKTRLGQSGDVDSGARGAKVLISSTQDLGSIDLTGKKISLKVMTLSNSKQKVNDVLG
                     NPVDIGIAVMWRVTDTAKAVFNVDNFKEYLSLQSDSALRNIVRNYPYDMAPGIDTTGD
                     GEPDEGSLRGSSSLVAEKIREEIQGRVEEAGLEIIDARITYLAYSQEIAAAMLQRQQA
                     SAIIDARMMIVEGAVGMVEMALEKLNENDIVELDEERKAAMVSNLLVVLCGSKDAQPI
                     VNSGSLY"
     gene            complement(1783..3576)
                     /locus_tag="VFD08_00160"
     CDS             complement(1783..3576)
                     /locus_tag="VFD08_00160"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZK09457.1"
                     /translation="MKGEKMLIEWVDEIKKLTQADKVHWCTGSDQEIKDLSEMMVKKG
                     IAEPLDEKEYPGCLLFRSDASDVARVEDRTFIASEKEEDAGPTNNWIDPKELKKTLTA
                     LFAGSMKGRTLYVIPFCMGPIDSPYSKLGVEITDSPYVVVNMAIMTRIGDRALERIKA
                     GEEFVPCLHSVGAPLQEGEEDIPWPCAPMEKKYIAHFPEERSIWSFGSGYGGNALLGK
                     KCFALRIASVLARDEGWLAEHMLILKLTSPEGKVYHVCGAFPSACGKTNLAMLIPTIE
                     GWKVETVGDDISWMHFGEDGKLYAINPESGFFGVAPGTSKESNPSAMATIQKNTIFTN
                     TALSDDKKVWWEGMGIDPPEHLIDWKGMDWTPKSKEKAAHPNARFTTPASQAPNIAPD
                     WQAPMGVPIDAILVGGRRATTIPLIHEAFSWQHGIFLGSIMGSEVTAAVISDKIGQVR
                     RDPFAMLPFIGYHVADYLQNWLDAAKKTQESKLPKIFYVNWFRKNDQGEFIWPGFGEN
                     SRVLKWITQRLDGSAPARKTPLGYIPSVEDLDLSGLDLNKEEFAEILSVDKEEWLKEV
                     ESIREHFDGYGEKMPKELYDELEALEKRLKG"
     gene            complement(3593..4291)
                     /locus_tag="VFD08_00165"
     CDS             complement(3593..4291)
                     /locus_tag="VFD08_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HZK09458.1"
                     /translation="MSNRKINWIVFDADDTLLDFKSAQKKAFTKMLAKLGLKDEGDYY
                     HTYTRLNSGLWRALEKGEMQKAELIDKRFGLFCQHYNLQGLDKKMKDLYEEELSKRGE
                     LIPGAWDLLEELSKSYPLALASNGILSIQRGRFRASGIRPFFKEIIVSEETGFEKPHK
                     GFFDFMMRKIGQSDPKKILFIGDGLNSDMLGALNYGLISCWYNPNKEHGRLKVDYSVQ
                     SLEQIPALVESLII"
     gene            complement(4313..4864)
                     /locus_tag="VFD08_00170"
     CDS             complement(4313..4864)
                     /locus_tag="VFD08_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZK09459.1"
                     /translation="MEMLKLIYAFVFTGLFAVGGGLATIPFLQDMGEKYGWFTTKELM
                     DMVAISESTPGPIGINMATFVGIRVGGYPGGVLATLALVFPSIVVVLILIPVLDKYRT
                     SALTQGIFYGLRPTSTALIAAALLGFLRMVVFLGEEIVLSGVNWLNILLFLGFFALIK
                     TKKTHPVLVIAIGAALGMILDLA"
     gene            complement(4851..5393)
                     /locus_tag="VFD08_00175"
     CDS             complement(4851..5393)
                     /locus_tag="VFD08_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZK09460.1"
                     /translation="MKIYIELFRTFFSMGLFTFGGGYAMLPLLQTEVVENKAWATEEE
                     LLDYYAIGQTTPGIIAVNTAVFIGQKQAGALGAVIAVLGLVSPSVVIISLLAGTLEEF
                     HTHPKVASALAGIRVAVSALILTTVIKMGKSAIRDRGGLIIFGIVLFLILGIKTPIIV
                     TVLLGAALGLVFTRRQDGNA"
     gene            complement(5667..6170)
                     /locus_tag="VFD08_00180"
     CDS             complement(5667..6170)
                     /locus_tag="VFD08_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZK09461.1"
                     /translation="MKKLIVLIETNFNEMELIYPYYRLLEDYEVHLVGTDQDVAYASA
                     AGLKMKSTHASRDIKAEDYAGLMIPGGFSPDYMRRSPETKALVKAMDEAGKPIAAICH
                     GPWLLASSLDLKGRKLTSFMAIKDDIVHAGANWVDEELVVDKNLFTSRTPADLPALMK
                     AFTKALK"
     gene            complement(6233..>6451)
                     /locus_tag="VFD08_00185"
     CDS             complement(6233..>6451)
                     /locus_tag="VFD08_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZK09462.1"
                     /translation="SLDLKDIESALSAVLATFNNIGPGLGKVGPSLNYYELSDLSKLI
                     LTFTMIAGRLEILPIFILFSPSTWKKNL"
BASE COUNT         1892 a         1545 c         1144 g         1870 t
ORIGIN      
        1 ctaatataga ctacgacagc attccccagt taatgacaca aatgcaagaa caccagatta
       61 actggtccat gcccaagatt catattaagg atgaaataga gctaaaccaa gtcctgcgca
      121 gtctaggagt caatcaaatc ttcgaagaag atggacagct gggaaatatt agcgaggaaa
      181 atttgcaagt ttcactaatt aaacagtgga ctgatcttct tgtggaagaa aaaggagtag
      241 aagcagccgc agcaacctta gttgaaatca aagccacctc cctgcctcca gaacttccag
      301 aagttgatat gaaactagat cgcccctttt ccctctttat tgtctttaga gatgttcccc
      361 tgttttttgg tgatgtctac cagccataaa gccctctaag acaagttgaa ccagtaaaaa
      421 aaggatgacc ttttaatagg ccatcctttt ttttatgatt ttactcttta gaggaaagat
      481 cctccggaag atatttccca tccttttttc tttgttttac acattgagta agaagatatt
      541 cgatttgccc attaatagag cgaaaatcat cttctgccca ggctgcaatc tgctcataca
      601 acttatggga gagacgaagg ggaatttgct ttttaggctt tgccatgact aatataaact
      661 accgctatta acaatgggct gggcgtcttt actaccacat aaaacaacaa gtagattaga
      721 aaccatggca gccttacgct cctcatcaag ttccacaata tcattttcat taagtttttc
      781 cagggccatc tccaccatcc ctaccgcccc ttctacaatc atcatccttg catcaataat
      841 tgcactagct tgctgccttt ggagcatggc tgcagctatt tcctgggaat aggcaagata
      901 ggtaattctg gcatctataa tctcaagacc agcctcttcc actctacctt gaatttcttc
      961 gcgaattttc tctgctacaa gactacttga tccgcgaagg cttccctcat cgggctctcc
     1021 atctccagta gtatcaattc ccggggccat atcataggga taatttctta caatattacg
     1081 cagggcacta tcactttgaa gggaaaggta ttctttaaaa ttatctacat taaagaccgc
     1141 cttagctgta tcggtaaccc tccacattac tgcaattcca atatccacgg gatttcctag
     1201 aacatcattt accttttgtt tggaattact cagggtcatc accttaagag aaatcttctt
     1261 gccggtaaga tcgatgcttc ctagatcttg tgtactggaa ataagaacct ttgctcctct
     1321 tgctccactg tccacatctc cactttggcc cagccttgtc ttagctgcag gatttaccgc
     1381 agtcgaaaaa gggttgacat agtaaaaacc aggctctttt atgcttcctg tataatttcc
     1441 aaaaagcgtt aaaacaatag cttccttggg cttaataatt ttaagtccaa aaaaaggaac
     1501 aatcccaaca agaagccaaa aaatcccccc agctaataat agacttccta ggctactacc
     1561 atcattcatc aaccgtatcc caaatataag ggctagaatg gccccaatat agagaagacc
     1621 taatagaata agtatgggca tgcccttttt gggcttcaaa attttttctt gcattgtcca
     1681 cctcctaata tctttatgat atcattttga tatcacttgt caaggagaaa ataagccaat
     1741 aaaaaagagg ctaccagcta tggcaacccc ttatctttat tactagccct ttagtctttt
     1801 ttccagggcc tctagctcat catagagctc ctttggcatt ttctctccat agccatcaaa
     1861 gtgctcacga atagactcta cttccttaag ccactcttct ttatcaacag aaagaatttc
     1921 tgcaaattct tccttattaa gatctaggcc cgaaagatcc agatcctcta cagaaggaat
     1981 atatcccaga ggagtctttc ttgcaggagc gcttccatca aggcgctggg taatccattt
     2041 aagaactctg ctattttcac caaagccagg ccaaataaat tctccctgat cattttttct
     2101 aaaccagtta acataaaaaa ttttaggcag cttactctcc tgggtcttct tagctgcatc
     2161 aagccagttc tgcagataat ctgctacatg atagccgata aagggcagca tagcaaaggg
     2221 gtctcttcgt acttgaccaa tcttatccga aataactgct gctgtaactt cacttcccat
     2281 aatggagcca agaaagattc catgttgcca actaaaggct tcatgaataa gcgggatggt
     2341 ggttgccctt cgacccccca ccaaaatcgc atcaatggga acacccatag gtgcttgcca
     2401 gtctggggct atatttggag cctggcttgc tggagtagta aaacgcgcat tgggatgggc
     2461 ggccttttct ttactcttag gggtccagtc catgcccttc caatcaataa ggtgctcagg
     2521 aggatctatt cccattcctt cccaccacac ttttttatca tcacttaggg cagtattggt
     2581 aaagatggtg ttcttttgga ttgtagccat agccgagggg ttgctttctt tgctggttcc
     2641 aggagccacg ccaaaaaagc ctgattctgg attgattgca tagagttttc catcctcccc
     2701 aaaatgcatc caggagatat catcccctac ggtttctacc ttccaaccct caatggtggg
     2761 tattagcatg gccagatttg tttttccaca ggctgaggga aaggcgccac agacatgata
     2821 taccttgccc tctgggctgg taagtttaag aatcagcata tgctcagcca gccaaccttc
     2881 atcgcgagca agaaccgaag caatgcgaag ggcaaaacac ttctttccta aaagagcatt
     2941 gcctccatag cccgagccaa aactccaaat gcttctttct tctggaaaat gggcaatata
     3001 ttttttctcc ataggagcac aaggccaggg gatatcttct tcgccttctt gtagaggagc
     3061 tcccacacta tgtaggcaag gcacaaactc ttccccagct ttaattcttt ctagggctct
     3121 atcgccaatt cttgtcataa ttgccatatt gactactaca taggggctat cagtaatctc
     3181 aacaccaagt ttagagtagg gagaatctat tggacccata caaaaaggaa tcacatatag
     3241 ggtccttccc ttcatagaac cagcaaacaa agcagtaaga gtctttttta actccttggg
     3301 atcaatccag ttatttgttg gtccagcatc ttcttctttt tctgaagcaa taaaagtccg
     3361 gtcttctacc ctggccacat cagaagcatc gcttctaaaa agcaggcagc ccggatattc
     3421 tttttcatct aggggctcag caatcccctt ttttaccatc atctcgctca ggtcttttat
     3481 ttcctgatca gagccagtac accagtgcac cttgtcagct tgggtgagct ttttaatctc
     3541 atcaacccac tctattaaca ttttttcgcc tttcactttt tcctccttta aattaaatga
     3601 ttagactttc aaccagagca ggtatttgct ctagggattg cacgctataa tcaactttta
     3661 gtctcccatg ctctttattg ggattatacc aacaacttat taagccataa ttaagggcac
     3721 ctagcatatc tgaatttaaa ccatcgccaa taaaaagtat cttctttgga tcgctttgcc
     3781 cgatctttct catcataaaa tcaaaaaatc ccttatgggg cttctcaaaa cccgtttctt
     3841 cggaaacaat gatttcttta aaaaaaggcc taataccact ggctctaaag cgtccccttt
     3901 gaatagaaag aatcccattg ctggctaagg caaggggata gcttttgctc aattcctcta
     3961 aaaggtccca ggctccagga attaactccc cccttttaga aagctcttct tcataaaggt
     4021 ctttcatttt tttatcaagg ccttgaagat tgtagtgttg gcaaaaaagg ccaaatctct
     4081 tatcaattag ctctgctttt tgcatttccc ccttttccaa ggctctccaa agaccactgt
     4141 ttaatctagt atatgtatga tagtaatcgc cctcatcctt taggcccagc ttggctagca
     4201 tctttgtaaa ggcctttttc tgcgcacttt taaagtcaag aagagtatca tcggcatcaa
     4261 atacaatcca gttgattttc ctattagaca tttgtttttt ccccttgtat ttttacgcaa
     4321 gatccagtat cattcccagg gccgctccaa tagctattac cagaacagga tgagtttttt
     4381 tcgtctttat caaggcaaaa aaaccaagaa aaagcagaat attaagccaa tttaccccac
     4441 ttaaaactat ttcttctccc aaaaacacaa ccatgcgcaa aaaacctaag agggcagcag
     4501 caattagggc cgttgaagtg gggcggaggc cataaaaaat cccctgggtt aaggcgctag
     4561 tgcgatattt atccagtaca ggaattaaaa taagcacaac tacaatagag ggaaaaacta
     4621 gggccagggt agccaaaaca ccacctggat atcctcccac tcttattcca acaaaagtcg
     4681 ccatatttat ccctatgggg cccggggttg attcgcttat agccaccata tccattagtt
     4741 cttttgtggt aaaccatccg tatttttctc ccatatcctg taaaaaggga atagtagcta
     4801 gaccaccacc tacggcaaaa agccctgtaa aaacaaaggc atagatcagt ttaagcattt
     4861 ccatcttgcc tcctagtaaa aacaagaccc agggcagcac ccaataaaac agtaacaata
     4921 atgggagttt ttataccaag gattaaaaat aagacaattc caaaaataat aaggcccccc
     4981 ctatctctta tggcgctttt acccatcttt attaccgtcg ttaaaataag agcacttacc
     5041 gcaacacgaa ttcctgccaa agcactggct accttagggt gagtatgaaa ttcttctagg
     5101 gttcctgcta ataaacttat aataacaaca ctaggagaaa caagacccag gactgcaatc
     5161 acagctccta gggcgccagc ttgtttttgc cctataaata ccgctgtatt tacggctata
     5221 atacctgggg ttgtctggcc aatggcgtag taatcgagaa gttcttcttc tgttgcccag
     5281 gccttgtttt caactacttc tgtttggagc agtggaagca tagcatagcc cccaccaaag
     5341 gtaaaaaggc ccatactaaa aaaagtccgg aataattcta tgtagatttt cataattcct
     5401 ccaaaagatt agaacttttt atttaatctt tcctatataa taccacttta cttatagaaa
     5461 aaatatctat acttctatcc ctttctaaaa gccaaggaaa tctagaagca taaaaaagtg
     5521 gcccaattta tatcgggcca ttttttattt ctctaacagg taagaccttg acatcctaaa
     5581 aaacgcagga ggagcttttt cacacaggtc ccagctaata aaagccaagg cttatcttgc
     5641 ctgcgttttt ccccaccctc ttcactttat ttaagcgcct ttgtaaaggc tttcattaaa
     5701 gcaggaagat ctgcaggcgt tctggatgtg aaaagattct tatccacaac aagttcctca
     5761 tccacccaat tggcaccagc atgaactata tcatctttta ttgccataaa agaggtcagc
     5821 tttcggcctt taaggtccaa agaagaagcc agaagccagg gaccatgaca aattgctgca
     5881 atgggttttc ctgcctcatc catggccttt actagggcct tggtttcagg gcttcgtcgc
     5941 atataatctg gagaaaaacc accaggaatc ataaggccag catagtcttc tgccttaata
     6001 tccctagagg catgggtgct tttcatttta aggccagcag cgctagcata ggccacgtcc
     6061 tgatctgtac caaccaaatg aacctcatag tcttctaaga gacggtagta ggggtaaatc
     6121 agttccattt cattgaagtt tgtttcgatt aaaacaatca gctttttcat tttacttcct
     6181 ttcgtattta gagtctatta ctaaatatac cccttctggc caaacaaaaa acctagagat
     6241 tctttttcca ggtagaagga gaaaagagaa tgaaaatcgg aagaatttct agtctgcctg
     6301 caatcatagt aaaggtaaga attagcttac taagatcact caactcgtaa tagttcaagc
     6361 taggccctac cttcccaagg ccaggcccaa tattattaaa ggtagctagg actgccgaaa
     6421 gagcactctc tatatccttt aagtctaaac t
//
LOCUS       DASRNA010000007         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_971824,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000007 DASRNA010000000
VERSION     DASRNA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971824"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFD08_00190"
     CDS             <1..644
                     /locus_tag="VFD08_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK09463.1"
                     /translation="VHVRGMNTKDESKLLEIRKTAGMIFQNPDNQIVATVVEEDIAFG
                     PENLGIPREEIGRRIDHVLKILGMEDYRFRPPHMLSGGQKQRIAIAGVLAMEPEIIIF
                     DESTSMLDPSGRKEVLKTIHQLHQQGITVVLITHFMEEVLGAQRVLVLEKGKLALDMK
                     PDELFMHMDLLKSYDLKVPEIIELAMELKKAGKDITFPIYNEMDLVRQLCPSD"
     gene            629..1471
                     /locus_tag="VFD08_00195"
     CDS             629..1471
                     /locus_tag="VFD08_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HZK09464.1"
                     /translation="MSIRLKDVSYTYNADSLYETTALDQITFEVGPGESLGIIGHTGS
                     GKSTLLQLLNGLLRPDSGYLEVAGIEISKSKKNLSALRKEVGLVFQYPEYQLFEETVA
                     RDIAFGPKNQGLSDEEVEKRVKESMELIGLDESYRDKSPLELSGGEKRRAAIAGVLAM
                     RPRILMLDEPTAGLDPQGRAQLIKQLMVLKGKLTLVFISHSMEEVAKIADRILVLSKG
                     KLILDDSVDKVFEEEKLLKEVGLDLPVIARLFARLREEGCPYKKNYFLLEDGLRVLME
                     GEDD"
     gene            1464..>2146
                     /locus_tag="VFD08_00200"
     CDS             1464..>2146
                     /locus_tag="VFD08_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZK09465.1"
                     /translation="MIKDMTLGQYYPVDSMIHRLDPRTKFIISFVYILAIFFLKEAYQ
                     YALLFIGLLGVIKLSKVPLAFIIRGIKPIVFILMFAFIMNLFLTPGEVLWSFWKITIT
                     KEGLRLAFLMMARLFLLIMGSSLLTLTTSPIKLTYALESLFSPLARFGFPVSEMAMMM
                     GIALRFIPTLTSETDKIMKAQMARGADFESGNIFRRAKSLIPILVPLFLSSIQRAIEL
                     ADAMEARCY"
BASE COUNT          629 a          412 c          487 g          618 t
ORIGIN      
        1 atgtccatgt taggggcatg aataccaagg atgaatcaaa acttcttgag attcgcaaga
       61 cggcgggaat gatttttcaa aaccccgaca atcagattgt tgcaaccgtt gtggaagaag
      121 atattgcctt tggacctgaa aacctgggta ttcccaggga agaaataggc cggcgtatcg
      181 atcatgtact aaaaatcctt ggtatggagg actatcgatt tagacctccc catatgctta
      241 gtggaggaca aaaacaacga attgccattg ccggtgtcct ggccatggag ccagaaatta
      301 ttatttttga tgaatcaacc tctatgcttg atcctagtgg tcgtaaagaa gtgctaaaga
      361 ccattcatca acttcatcag caggggatta ccgttgtact tattactcat tttatggaag
      421 aagttttagg agcccagcga gttttggttc tggaaaaggg aaaactggcc ctggatatga
      481 aacccgatga actttttatg catatggatc ttttaaagag ctatgacctt aaagttccag
      541 aaataattga gctggccatg gaactaaaaa aagcaggaaa agatataact tttcctattt
      601 ataatgaaat ggatcttgtg aggcaattat gtccatccga ttaaaggatg tatcttatac
      661 ctacaatgca gactcccttt atgaaacaac agccttggac cagattactt ttgaagtagg
      721 tccaggagaa agcctaggca taataggcca tacaggcagt ggtaagtcca ccttactcca
      781 gctgttaaat ggacttctaa gaccagatag tggctattta gaagtagcag ggatagaaat
      841 atcaaagtct aagaaaaact tatcggccct tcgaaaagaa gtaggccttg tttttcaata
      901 cccagaatac cagctctttg aagaaacggt tgctagggat atagcctttg gaccaaaaaa
      961 ccagggcctt agtgatgagg aagtggaaaa acgtgtaaaa gaatccatgg agcttatcgg
     1021 ccttgatgaa agctacaggg acaagtctcc cctggaactg agtgggggag aaaaaaggcg
     1081 agctgctatt gcaggagttt tggccatgcg tccaagaatc ttaatgttag atgaacccac
     1141 agcgggtctt gacccccagg gaagggccca gcttatcaag caactgatgg ttctaaaagg
     1201 aaaacttacc ctggttttta tctctcactc catggaggaa gttgccaaaa tagcagatag
     1261 aatcctggtc ctctcaaagg gtaagttgat cttagatgat agtgtagaca aggtatttga
     1321 agaagaaaag ctgttaaagg aagtgggcct tgatctgcct gttatcgcgc ggctttttgc
     1381 ccgcctaaga gaagagggtt gtccttacaa gaagaattat tttcttcttg aagatggact
     1441 tcgcgttctt atggagggag aagatgatta aagatatgac cctgggtcaa tactacccag
     1501 tagactctat gattcatcgt ttggatcccc ggaccaagtt tatcatcagc tttgtctata
     1561 tactggctat tttcttttta aaagaagcct accagtatgc cctcttattt attggccttc
     1621 ttggtgttat taaattatct aaagttcccc tggcctttat tataagggga atcaaaccaa
     1681 ttgtttttat tttaatgttt gcttttatta tgaatttatt tcttactcca ggggaggtcc
     1741 tttggagctt ttggaaaatt accatcacaa aagagggact aaggttggct tttcttatga
     1801 tggcaaggct attcttactg attatgggga gttctttatt aactctaaca acaagtccca
     1861 tcaagctgac ctatgccctg gagagtcttt tttctcccct ggccaggttt ggatttcctg
     1921 tttctgagat ggccatgatg atgggaattg ccctgcggtt tatccccacc ctaacatctg
     1981 agaccgacaa aataatgaag gcccaaatgg ccaggggggc tgattttgag agcggaaata
     2041 tttttaggcg ggcaaaatca cttataccaa tccttgttcc acttttttta tcttccatac
     2101 aaagggccat tgagctggct gatgctatgg aagcaaggtg ttatca
//
LOCUS       DASRNA010000008         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_896796,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000008 DASRNA010000000
VERSION     DASRNA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896796"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFD08_00205"
     CDS             <1..1243
                     /locus_tag="VFD08_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZK09466.1"
                     /translation="SINPDDKQWKKAIAPSIENNARVRFLVFDSGQKLISDSYNFLRA
                     GESLSLELVEEVNKENSFEHRLYKNSKDENLLYTAYPVYNEEEKPLATIVAMTSLGEP
                     IEALSILLQRIIIIGIIFIISSLVIYFWILRSIFTPIDRLEKGVKAMTRGNYSYQIEE
                     DQESQLDPIVRSFNTLGSRLGNIEVQQKDFVSTLSHELKTPIASMKIITDSLIHAKDT
                     VEKEVLYDFLEDINSESDRLKDIIDDLLFMASLERQDVSLNLEVRAITMALEESVRVV
                     QPLADQKDIALYLEPHEELFAEFDYNKMKQVFINIIGNAVKYTDEGGSVFISSSADKN
                     GVYIEVQDTGIGVDPEDLPYIFDRFYRVEMARSRLRGGTGLGLHIVHNIVNLHNGKIY
                     MESESDVGTTVYIELPRVYEV"
     gene            1245..2111
                     /locus_tag="VFD08_00210"
     CDS             1245..2111
                     /locus_tag="VFD08_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09467.1"
                     /translation="MKKARNYFLILILMFLMINIYFYLNFHKPYPLEPYDLSVVDDHR
                     YMSYPVYFVGDDRLVKSREYIEVIWRDDQENVQDVYFAQAKLPIEGRSIQILNISIDK
                     LTVTPEHIYIYTQLSSFRDARYNRDNFYLYLMSFVNTLTENSKEKTVYFILDGRISSP
                     ELYGVDMNQGFSYDLSLVAEDKDKVAKNMRQFFREMYLQDYERAYKKLSREIRESHRI
                     AGYAKAFESYVYNRNNDFPWDFIVEKNQDKYSVTVHFGPESNYPDEVWGVDIVDGIFI
                     VDYFPELLNYLP"
     gene            complement(2154..2250)
                     /locus_tag="VFD08_00215"
     tRNA            complement(2154..2250)
                     /locus_tag="VFD08_00215"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Sec,seq:tca)
     gene            complement(2251..2829)
                     /gene="plsY"
                     /locus_tag="VFD08_00220"
     CDS             complement(2251..2829)
                     /gene="plsY"
                     /locus_tag="VFD08_00220"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HZK09468.1"
                     /translation="MNIIVSIIVGYFLGGLPFAYVIGKIQKNIDLRKVGSGNLGATNV
                     FRNLGSVSFAICAFFDIGKAFLAFHIGLALFGQPYGFVAGMASVIGHCYSPFLKFKGG
                     KGVAASGGLLLAYDWRIFIVALFLMIIILFISRRMSLASIGAALSTPLIYYLFYGFDL
                     LIFGAIILAGFLVFQHRDNITRLLNGTEAKLF"
BASE COUNT          985 a          521 c          604 g          868 t
ORIGIN      
        1 gtctattaat cctgatgata agcagtggaa aaaagcgata gcaccctcaa ttgaaaacaa
       61 tgcaagggtg cgttttttgg tatttgattc tggccagaag ctaattagtg atagttataa
      121 ttttcttaga gctggtgaaa gtttatctct agaattagtt gaagaggtga ataaagaaaa
      181 cagttttgaa catcgcctat ataaaaacag caaggatgaa aacctacttt atactgccta
      241 ccctgtctac aatgaggaag aaaagcctct tgctacaatt gtagcaatga caagcctggg
      301 tgaacccatt gaagccttgt ctattctttt gcaaagaatt attattattg gcattatatt
      361 tattatttct tctctagtca tatatttttg gattttaaga agtattttta ctcctattga
      421 tagactggaa aaaggagtaa aagctatgac tagaggaaat tatagctatc aaattgaaga
      481 agaccaggaa tcacaacttg accccatcgt tcgctctttt aatactttgg gttcgcgcct
      541 tggtaatatt gaggtccaac aaaaagattt tgtttccacc ttaagccatg aattaaaaac
      601 acctatcgct tcgatgaaaa ttattacgga ctctctaatc catgcaaaag atacagtgga
      661 aaaagaggtt ctttatgatt ttcttgagga tatcaactct gagagtgaca gattaaagga
      721 tataattgat gatcttttat ttatggcttc tctggagcgg caagatgtgt ctttgaatct
      781 tgaagttcga gcaattacta tggcactgga ggaatcagta cgggttgttc aacccctggc
      841 tgatcagaag gatattgctt tgtatcttga gcctcatgaa gagctttttg ctgaatttga
      901 ctacaataaa atgaaacaag tttttataaa tattattggc aatgctgtca aatacactga
      961 tgagggggga agcgttttca ttagcagctc tgctgataaa aacggagtct acattgaagt
     1021 gcaagatacg ggcattggag ttgatcctga ggatctcccc tatatctttg ataggtttta
     1081 tagagtggag atggccagaa gtagactaag aggcggtaca ggtctaggtc ttcatattgt
     1141 tcataatatc gtaaatcttc ataatggaaa aatatatatg gagtctgaaa gtgatgtagg
     1201 aaccacggta tatattgaac ttccccgagt atatgaggtg taggatgaaa aaagcacgta
     1261 attattttct gatactgata ctaatgttcc ttatgatcaa tatttatttc taccttaatt
     1321 ttcacaagcc ttatcccttg gagccttacg atttgagtgt ggtagacgat catcgatata
     1381 tgagctatcc ggtatatttt gtgggagacg accgccttgt aaagagccgg gaatacatcg
     1441 aggtaatttg gcgggatgac caggagaatg tgcaggatgt gtattttgct caggccaagc
     1501 ttcccattga aggtcggagt attcaaattc tgaatatttc tattgataag ctaacggtaa
     1561 ctcctgagca catctatatt tatacacaac tttcaagctt tagagatgct cgctataatc
     1621 gcgataactt ttatttatat ctaatgagct ttgttaatac ccttactgaa aacagtaaag
     1681 aaaaaacagt gtattttatc ttagatggaa ggatttcttc tccagagctt tatggagtcg
     1741 atatgaatca gggctttagt tacgacttat cccttgtggc agaagataaa gacaaagtag
     1801 caaagaatat gaggcagttt tttcgagaaa tgtacttgca agattatgaa agagcctata
     1861 agaaactctc aagagagatt cgagaaagcc atagaatagc aggctatgct aaggcctttg
     1921 aaagctacgt atataatcga aataatgact ttccttggga ttttattgtt gaaaaaaacc
     1981 aggacaaata tagtgtcact gtgcactttg gtccagaaag caattatcct gatgaagtct
     2041 ggggagtgga tattgttgat ggcatattta ttgtggatta ttttccagaa ctcttgaact
     2101 acctaccata aataaagaaa aaaggcagct tcctaagaaa ctgccttttt atatggcgga
     2161 aatggatggg aatcgaaccc acctaggatg ctcctaacac cctacactgg ttttgaagac
     2221 cagaaggggc accagccccc taccatctcc ttaaaataat ttggcttctg ttccatttaa
     2281 gagtcgagtg atattgtctc gatgctgaaa tacaagaaac cctgctaaga ttatagcacc
     2341 aaatattaat aagtcaaagc cataaaacaa atagtagata aggggagtag aaagggccgc
     2401 accaatagag gcaaggctca tcctgcggct gataaaaagg attataatca taagaaaaag
     2461 tgctacaata aaaatcctcc agtcataagc caaaagtagg cctcctgaag cagctacccc
     2521 cttgccacct ttaaatttta aaaaaggtga gtagcagtgg ccaataacag aagccattcc
     2581 ggcaacaaaa ccgtagggct ggccaaatag agcaagccct atatgaaagg ccaaaaaggc
     2641 ttttcctata tcaaagaagg cacaaatagc aaaggagaca gagcccaggt tgcgaaagac
     2701 attggtagct cccaaattcc cactgccaac tttacgcaaa tcaatatttt tttgtatttt
     2761 tccaattaca taagcaaagg gcaggccacc tagaaagtaa cctacaatta tgctaacaat
     2821 tatattcatt attacctcag attgattgta cggtagtgta aaatatcttg tgtaaacccc
     2881 tcatcattct atctatagaa aagagagcgc caaccttgta taatgttaag tgacaaaaca
     2941 aaacagaaac agggaaggag ccctctcatg agttattt
//
LOCUS       DASRNA010000009         8097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1046041,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000009 DASRNA010000000
VERSION     DASRNA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046041"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..104)
                     /locus_tag="VFD08_00225"
                     /pseudo
     CDS             complement(1..104)
                     /locus_tag="VFD08_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950505.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(154..1374)
                     /locus_tag="VFD08_00230"
     CDS             complement(154..1374)
                     /locus_tag="VFD08_00230"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HZK09469.1"
                     /translation="MKNYNTVKKYFESILINYPCEKATLSLHIDYVKIVKSYEDKSYI
                     GLEAISTSTEGVCKHCGQVISRFKQYKTTYTTVAKFNNKNIIVKLKKKMYKCPDCLTC
                     STEKLLDCSGRNQKTNSFIRSMLEYLKETMSFSAVARLHKVSVNNVIRHFDKVNLTET
                     AIDRTKIKNISVDEVRFVKQKYANYQFVIIDSDKKIVLDVLPTRQQKYVKDYISKHYS
                     GIETFTQDLWRPYRNIAYGLFPTTKVIADRFHVVRQFMWAFSRTRIALAKEQKKATNK
                     NWRLLTKAQKDLSEKGKQKLEQLLAEDPQLHLAHRAKEMALELYRCRNSQSYLELLPT
                     FKELIDSNHLLEFQKAYSSTQNWHDEIVNMFDYPYSNGSMERINRAIKQSKNVAFGFT
                     NLSRATKLIQYRIN"
     gene            complement(1542..2027)
                     /locus_tag="VFD08_00235"
     CDS             complement(1542..2027)
                     /locus_tag="VFD08_00235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZK09470.1"
                     /translation="MNPKMRLSLENGEEMILELYPEMAPKTVDNFIHLVEKGFYDGLG
                     FHRIIPGFMIQGGCPDGTGMGGPGYAIPGEFKSNGFDNPLKHDRGVLSMARSMMPDSA
                     GSQFFIMHKNSPHLDGDYAGFGRVIEGLEVLDKIASTPTGAQDKPLEPVVIERIDIIE
                     D"
     gene            complement(2127..2540)
                     /locus_tag="VFD08_00240"
                     /pseudo
     CDS             complement(2127..2540)
                     /locus_tag="VFD08_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
     gene            complement(2485..3105)
                     /gene="yihA"
                     /locus_tag="VFD08_00245"
     CDS             complement(2485..3105)
                     /gene="yihA"
                     /locus_tag="VFD08_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZK09471.1"
                     /translation="MKITSATFLGSAVGRDQFPPNDMPEFAFAGRSNVGKSSLINYLL
                     NRNKLARTSSTPGKTQTINFYEINGQFRFVDLPGYGYAKVSKTEKAKWAGFIDQYLRE
                     RENLMEIFFLLDIRRKIKEEDIQMLEYIRSLGYEGRIILTKIDKLNQSQLVHARWDIE
                     EKLGVPREHTFIISNLKSKGKYPIWECMNGLFDKHGVPIHLDRQNA"
     gene            complement(3102..5402)
                     /gene="lon"
                     /locus_tag="VFD08_00250"
     CDS             complement(3102..5402)
                     /gene="lon"
                     /locus_tag="VFD08_00250"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZK09472.1"
                     /translation="MNKRIPLIPLRGLIIFPYMVLYFDVGREKSVAALEQAMLQDQLI
                     FLTTQKDMDVENPLEGDYYHSGTIAKIKQMLKLPGDAVRVLVEGQERARVTNFTPERN
                     FIEVEYEILEDQVADDLESKALERSIKETFKKYSSLSNRIPSELSISLSGVEEPGRFA
                     DLIIPQLNITIEEKQALLETLDTKKRLFKLYHHLLQEVEIVELEKDLSEKVKEQMNKS
                     QKEYFLREQMRIIQQELGDEGDIASEAEKLKEKLKKLRLKKDINEKMEKEIDKYSRLN
                     PSSPEAAVSRTYIQWILDLPWNKQSKEQIDLPHAREVLDEDHYGLEKVKERVLEYLAV
                     RKLTGSMKGPIICLVGPPGVGKTSIASSIARSVNRKFIRMSLGGVRDEAEIRGHRRTY
                     IGAIPGRIMTHIKESGVKNPLFLFDEIDKLGSDFRGDPASGLLEVLDPEQNKTFQDHY
                     LEVPFDLSKVMFITTANTTSTIPRALLDRMEVIDITGYTLYEKVQIAERYLIKKQQKE
                     NGLEKADIKFSKEGLRTLIEEYTRESGVRELERKIGTICRKIAIEVVESKKEKFSVTK
                     SSLSKYLGEPPYKADELEKEDRVGLVNGLAWTSFGGDTLEIEVNVSPGKGVLSLTGQL
                     GDVMKESAQAGLSYLKSQSEELDIKEDFFSKHDIHVHVPAGAIPKDGPSAGITMATAM
                     ASAITNRKASHTLAMTGEITLRGRVMAIGGLKEKLLAAKRRGIKRVVIPKDNERNLKE
                     VPREIKKDLEIIPVSSMEEVLKEALK"
     gene            complement(5399..6610)
                     /gene="clpX"
                     /locus_tag="VFD08_00255"
     CDS             complement(5399..6610)
                     /gene="clpX"
                     /locus_tag="VFD08_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZK09473.1"
                     /translation="MQDKKKTCSFCGRGEDEVLGLISSPKAAICDECVEQCGELFFEN
                     SFKENFSPGVDKESVPKPKEIKSFLDEYVIGQDEAKKILAVAVYNHYKRIGNTTDVEI
                     QKSNILLLGPTGSGKTYLAQTLAKILDVPFAMADATSITEAGYVGDDVENVLLKLLHA
                     ADFDVESAQRGIIYIDEIDKIARKSESTSITRDVSGEGVQQALLKLIEGTVASVPPQG
                     GRKHPHQEMINVDTQNILFILGGAFEGLTEIVARRQGRGSVGFHAPIKSKSEKSVWNL
                     LNQVEGRDLQNFGLIPEFIGRVPILAPLHELNQEILEKILTEPKNALLRQYEALFSMD
                     DIELSLDKEALKEIAAQAVNRGAGARGLKHILEKIMLEYMFEYPSKKGVDKLHLTIKE
                     VEEKLEIKEAL"
     gene            complement(6611..7195)
                     /locus_tag="VFD08_00260"
     CDS             complement(6611..7195)
                     /locus_tag="VFD08_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZK09474.1"
                     /translation="MNLVPIVIEKTAQGERSYDIYSRLLRDRVLFISGVIDDNLANSV
                     VAQLLFLEKEDPDAPIHIYINSVGGSVSAGFAIHDTMQFINCPVSTICVGMAASMGAF
                     LLAAGSKGMRYALPNSEIMIHQPLGGVQGQAEDIRITAEHIIELRKRINTLLSKYTGK
                     TLKKIEKDTDRDNYLSALGALEYGIIDEVLEKRK"
     gene            complement(7195..>8097)
                     /gene="tig"
                     /locus_tag="VFD08_00265"
     CDS             complement(7195..>8097)
                     /gene="tig"
                     /locus_tag="VFD08_00265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZK09475.1"
                     /translation="IEAVDTKVSDEEVMDKLKEEAEKNARMVPVDVAQEEDIVTIDFD
                     GSIDGEKFDGGEGNEFDLTLGSQQFIPGFEEQLIGKSAGEHEVKVNFPEDYGEESLAG
                     KEALFKVVIHDIKRKEIPELDDELASDLSEFESLEEYKQELRELLEKEKEELAENKKA
                     DDALIKAIENMEVELPEAMVEDGVDRKFQEMDHQLSQQGFGLAQFLEMSGQSVEDFRS
                     NYRPQVELDIKRELLLEAIVKEENFEISQEEVDEVIKEQVETHKMEEEAIRKIYEQDD
                     YKYLKHGLKMEKAQKLILESVEVV"
BASE COUNT         2029 a         1868 c         1539 g         2661 t
ORIGIN      
        1 tggtaattta atagagacag cataggtaca aaaattggag ctaagagggc ccattttgcg
       61 gagccagaac cgataaagag atttacaaag gtccaaagta aaacggtact ttgtcaattt
      121 agtgggcaag taaaaaattc agttaattaa ttcctagtta atacgatact gaattagctt
      181 cgttgcccgg gagagattgg tgaaaccaaa agctacattc ttggattgct tgatggcacg
      241 gtttatccgc tccatagatc cgttagagta aggatagtca aacatattta ctatttcatc
      301 atgccagttt tgagtgctcg aataggcctt ttggaattcc aacagatgat tgctatcaat
      361 cagttctttg aaggtaggca gaagttctag gtagctctgg ctattcctgc atctatatag
      421 ctcaagtgcc atttcttttg ctctatgggc taaatgtagc tggggatctt cagcaagaag
      481 ttgttctagc ttttgctttc ctttttcgct caggtctttt tgcgccttcg ttaatagtct
      541 ccagttttta ttggtcgctt ttttctgctc tttagcaagt gcaatcctag ttctagaaaa
      601 agcccacata aattgtctga cgacatggaa gcgatcagcg attacctttg tggttgggaa
      661 taagccatag gctatattcc ggtatggcct ccaaaggtct tgtgtaaagg tttctatccc
      721 tgaataatgt ttcgatatgt agtcttttac gtatttttgc tgccttgtag gaagaacatc
      781 caaaactatt ttcttatcag aatcaataat tacaaactga taatttgcat atttctgttt
      841 cacaaaacgc acttcatcga ctgaaatgtt tttgattttt gttcggtcta tagcggtttc
      901 ggtaagattt accttgtcaa aatggcgaat cacgttattt acagatactt tatggagtct
      961 agccacagca gaaaaagaca tagtttcctt gagatattca agcatggatc gaatgaaact
     1021 gttggttttt tgatttcttc cagagcagtc gagtaatttt tctgtactgc aagtcaggca
     1081 atcagggcac ttgtacatct tcttttttaa ttttacgatg atgttcttat tattaaattt
     1141 agctactgtc gtataggtcg ttttatactg cttaaatctt gagatgacct gtccgcagtg
     1201 tttacagaca ccttcagtag aagtagagat tgcttcaagc ccgatatagc ttttgtcctc
     1261 gtagctcttc acaatcttta catagtcgat gtgtaaagat agcgtcgcct tttcacaagg
     1321 ataattgatt aatatagatt caaaatattt ctttactgtg ttataatttt tcatggagtc
     1381 cacctctgtt gattttatgg tttaggttgc actaactatt ttaaatcaac ttaggatgtg
     1441 actccactca ttttttaatg atttgcccac ttaattatta tagaaccgaa aaaaacaggc
     1501 tgattattct gccaatcaac ctgcctactt gttagagaaa actagtcctc tattatatca
     1561 atgcgctcaa tcactaccgg ctcaagaggt ttatcctggg ctcctgttgg ggtagaagca
     1621 attttatcta ggacttcaag tccttcaatc acccggccaa agcctgcata gtctccatca
     1681 agatggggag aattcttatg cataataaaa aactggctac cagcagaatc tggcatcata
     1741 cttcttgcca tactaagaac gcccctatca tgctttaggg gattgtcaaa gccattgctt
     1801 ttaaactctc ctggaatagc gtagccaggc ccacccatac cagtgccatc aggacagccg
     1861 ccctggatca taaagcctgg gataattcgg tggaatccta gtccatcata aaaacctttt
     1921 tctaccaaat gaataaagtt atctactgtt ttaggagcca tttcaggata aagctccaag
     1981 atcatttctt caccattttc gaggcttagc ctcatttttg ggttcatctt tcctccaaat
     2041 ataacgtcat agtttttaaa tacaccctct ttataataga aaactgcctg gaaaaaggga
     2101 ccatcctgga ggatccaggg taaaaagacc ctatctccct cccagagggc aaggtctaag
     2161 accttgtctt tttccaccca gactggctgg ccttcgtgat tttcactaag ctggccctgg
     2221 gttttttcca cccggtaaac gtaactaaag atatcatttt ctccatcaaa attaggaaaa
     2281 aatagggtcc caaccagctt gtcttcaact acttgcaggc ctgtctcttc caagacctcc
     2341 cgcttcatac actcccgggg gctttcgcct ttttctacct tgccaccagg tcctacatat
     2401 ttgccatagt ggacatcatt ctcctcctta ttgcggtgga gcataaggac cctgcccccc
     2461 tccacaatat aaaccaaggt ggttctaagc attctgcctg tccaaatgta tagggacccc
     2521 atgtttatca aagagtccat tcatgcactc ccaaatagga tacttaccct tggacttgag
     2581 attgctgata ataaaagtat gttctctggg aacacccagc ttttcttcaa tgtcccagcg
     2641 agcatgaaca agttgggact ggttgagctt atcaatcttg gtcaggataa tgcgcccctc
     2701 ataaccaaga gagcgaatat attctaacat ctgaatatct tcttccttga tttttcttcg
     2761 aatatcaaga agaaaaaaga tctccataag attttctctt tcacgtagat actggtcgat
     2821 aaagcccgcc cacttggcct tttctgtctt agaaaccttt gcatagccat agccgggaag
     2881 gtccacaaaa cgaaactgtc cattgatttc gtaaaaatta atagtttggg tttttcccgg
     2941 tgtggagctt gttcttgcca gcttattgcg attgagcaaa taattaatta gggaactctt
     3001 gcccacattg gaccggcctg caaaagcaaa ttctggcata tcattgggag ggaattggtc
     3061 cctcccaaca gcactgccta aaaaagtggc ggaagtgatt ttcacttcaa ggcctccttt
     3121 aatacttctt ccatggaaga aacaggaata atttctagat cctttttaat ctcccgtgga
     3181 acttccttca gatttctttc attgtcttta ggaataacta ctcttttaat tcccctgcgc
     3241 ttggcggcca ggagtttttc cttaaggccc ccaatagcca tcaccctacc acgaagggta
     3301 atctcccctg tcatagccag ggtatgggac gcttttctat ttgtgatggc agaagccatg
     3361 gctgttgcca tggtaattcc cgcactgggc ccatcctttg gaatggctcc tgcaggaaca
     3421 tgcacatgga tatcatgctt agaaaagaaa tcttccttga tatcaagttc ttctgactgg
     3481 ctctttagat agctaagacc ggcctgggcc gattctttca ttacatctcc tagttgacca
     3541 gtaagggata agaccccctt gccaggagaa acatttactt caatttctag ggtgtctcca
     3601 ccaaagcttg tccaggctag tccatttact aggcctaccc tgtcttcttt ttctagctcg
     3661 tccgccttat agggcggctc tcccagatac ttgcttaggc tggactttgt cacagaaaac
     3721 ttttcttttt tgctttcaac gacttcaatg gctatttttc tacagatggt tccaattttt
     3781 ctttccagct ctcttacacc tgattctcta gtatattctt caattagggt gcgaaggcct
     3841 tcttttgaaa atttgatatc tgccttttct agtccatttt ctttttgttg tttctttatt
     3901 aaatagcgct cagcaatctg caccttttca tagagggtgt agcctgtaat atcaatcact
     3961 tccatcctat caagcagggc tctggggatg gtggaggttg tattggctgt agtaataaac
     4021 ataaccttac ttagatcaaa gggaacttcc agatagtggt cttggaaggt cttgttttgt
     4081 tctggatcta gcacttctag tagacctgaa gcgggatctc ccctaaagtc tgagccaagt
     4141 ttatctattt catcaaagag aaagaggggg ttttttactc cactttcttt tatatgggtc
     4201 ataattcgtc cgggaatagc cccaatatag gttctacggt ggccgcgtat ttctgcctca
     4261 tctcgcactc cacccagact catgcggata aatttgcggt taacgcttct agcaatacta
     4321 gaggcaatgg aggtttttcc cacccctgga ggacccacaa gacaaataat gggacccttc
     4381 atactgcccg ttagttttct caccgccaga tattctagga ctctttcctt gaccttttca
     4441 agaccatagt ggtcttcatc aagaacctcc ctggcatggg gtagatctat ttgctcttta
     4501 ctctgcttgt tccagggaag atcaagaatc cactggatat aggttcttga tacggctgcc
     4561 tctggggaag agggattgag tcttgagtat ttatcaatct ctttttccat cttctcattt
     4621 atatcttttt tcaggcggag ttttttgagt ttttctttta gtttttctgc ttcacttgct
     4681 atatcaccct catcgccaag ctcttgctgg ataatgcgca tttgctcgcg aagaaaatat
     4741 tctttttggc tcttattcat ctgctctttt actttttcgc ttagatcttt ttctagctca
     4801 actatctcca cttcttgcag caggtggtga tagagtttaa agagtctttt tttggtatcc
     4861 agggtttcaa gcagggcttg tttttcctca atagtaatat tgagctgggg gataattaaa
     4921 tccgcaaaac ggcccggctc ctctactcca gaaagagaaa tacttagctc actggggatg
     4981 cggttactaa gagaggagta ctttttaaag gtctccttga tgctgcgctc cagcgccttg
     5041 ctctcaagat catccgctac ctgatcttca agaatttcat attctacttc aataaagttt
     5101 ctttcagggg taaagtttgt tacccttgct ctttcttgtc cttctactaa aactcgaaca
     5161 gcatcacctg gaagctttaa catctgctta atcttggcaa tggtgccaga gtgataataa
     5221 tctccttcaa ggggattttc gacgtccata tccttttgtg tggttaaaaa gattagctgg
     5281 tcttggagca tggcttgctc cagggctgct acactctttt ctctacccac atcaaaatac
     5341 aggaccatat agggaaatat aatcagtccc cgaagaggaa ttaatgggat tctcttattc
     5401 ataatgcctc cttaatttct agtttttctt ctacttcttt tatggttaaa tggagcttgt
     5461 ctacgccttt tttgctggga tactcaaaca tatactctag cattattttt tctaaaatat
     5521 gctttagtcc ccgggcgcca gctcctctat ttactgcctg ggctgcaatt tcctttaggg
     5581 cttctttatc caggcttagc tcgatatcat ccatggaaaa aagggcttca tactggcgca
     5641 aaagggcatt tttgggctca gtaagaattt tttctaggat ctcttgattg agttcatgaa
     5701 ggggggccag gataggcacc cgtccaataa attcaggaat taggccaaag ttctgcaggt
     5761 ctctaccctc tacttgattg agtaaattcc agacgctttt ttcactcttg ctctttatgg
     5821 gagcatgaaa accaacgctg cctcttccct gcctgcgggc cacaatctcg gttagcccct
     5881 caaaggcgcc ccccaggata aagagtatat tttgtgtatc aacattaatc atctcctgat
     5941 ggggatgttt tctgcctccc tggggaggaa cagaggctac tgtaccctct attagtttaa
     6001 gaagagcctg ctggacacct tctccactta catcacgagt aatcgaggta gattcgcttt
     6061 ttcgagcgat cttatcgatc tcgtctatat agatgatacc cctttgggcg ctctctacat
     6121 caaaatccgc tgcatgaagt agttttagta ggacattttc cacatcatct cctacataac
     6181 cagcctcggt aatggaggta gcatcggcca tggcaaaggg aacatccagg atttttgcca
     6241 gggtctgggc tagataggtt tttcctgaac ccgttggccc cagaaggagg atattgcttt
     6301 tttgaatctc cacatcggta gtatttccaa tgcgtttata gtggttgtag acggctacag
     6361 caagaatttt tttcgcctca tcttgaccaa ttacatactc atcaagaaag gacttaatct
     6421 ccttgggctt tggaacagac tccttgtcca ccccgggaga aaaattctcc ttaaagctat
     6481 tctcaaaaaa gagttcaccg cactgctcaa cgcactcatc acagatagcg gccttaggtg
     6541 aactaattag tcctaggacc tcatcttcgc cgcgcccgca gaaggaacat gtctttttct
     6601 tatcttgcac ttatttcctc ttttcaagga cctcatcgat aattccatat tctagcgctc
     6661 caagagcact tagatagtta tctcgatccg tgtccttttc tattttcttt aaagtcttgc
     6721 ctgtatactt gctaagcagg gtattgattc tttttcttag ctcaataata tgctccgccg
     6781 tgatccggat atcctcagcc tgaccctgga cgccccctag gggctggtgg atcataattt
     6841 cgctattggg cagggcgtag cgcattccct tgctgcctgc agcaagaagg aaagcaccca
     6901 tggaggctgc catacctaca caaatagtag atacaggaca attaataaac tgcatggtat
     6961 catgaatggc aaatcccgct gaaacagatc cccccaccga attaatataa atatggatgg
     7021 gagcatcggg gtcttctttt tctaaaaaga gtagctgggc tacaacggaa ttggctaggt
     7081 tatcatcgat tactccagaa ataaagagca ccctatcccg aagcaggcgg gagtagatat
     7141 catagctacg ctctccctgg gctgttttct cgattacaat aggaactaga ttcactagac
     7201 tacctctaca ctttcaagga ttagcttttg tgctttttcc atttttaagc catgcttaag
     7261 atacttatag tcatcttgct catagatttt tcgaatggct tcttcttcca tcttgtgagt
     7321 ctccacctgc tcttttataa cttcatctac ttcttcttgg ctaatttcaa agttttcttc
     7381 ttttacaatg gcctctagca ggagctctct ttttatatcc agctctactt ggggcctata
     7441 gttgcttcta aagtcttcca cactttggcc tgacatttct aaaaactgcg ctaggccaaa
     7501 gccttgctgg ctaagttgat ggtccatttc ctggaacttt ctatctactc catcttctac
     7561 catggcctct ggaagttcta cctccatatt ttcaatggcc ttgataagag cgtcatctgc
     7621 ttttttattt tctgctagct cttctttttc tttttctagc agctctctta gttcttgctt
     7681 atattcttct aagctttcaa attctgatag atctgatgcg agttcatcat caagctctgg
     7741 tatttctttt cttttaatat catgaatgac taccttaaag agggcttctt ttcctgctag
     7801 gctctcttcg ccataatcct caggaaaatt aacctttact tcatgctctc ctgctgattt
     7861 tcctattagc tgctcttcaa agcctgggat aaattgctgg ctaccaaggg tcagatcaaa
     7921 ctcatttcct tcgcctccat caaatttttc cccatcaatg ctaccatcaa agtctatagt
     7981 cactatatct tcttcttggg ccacatcaac tggcaccata cgggcatttt tttctgcttc
     8041 ttcttttagt ttgtccatta cttcttcatc acttaccttt gtatctactg cttctat
//
LOCUS       DASRNA010000010         5216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_151391,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000010 DASRNA010000000
VERSION     DASRNA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151391"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1265
                     /locus_tag="VFD08_00270"
     CDS             87..1265
                     /locus_tag="VFD08_00270"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZK09476.1"
                     /translation="MKKVVVVAAVRTPIGDFLGFLQSVGSVELGEIAVKGALKQAGIE
                     PEMVEELACGMIYKAGNKGNPARQVQIKTGMPVEGSAYTIDQQCGSGMKAFELIHQSI
                     MLGKIDIGVALGSESMSQAPYLLKTLRQGQRMGDAKALDSMLEDGLICALEGYHMGVT
                     AENLAERYGISRQEQDELALLSQKRAVKAIAEGKFKDEIVPVVIQTRKGEILVDTDEH
                     PRKDISLEKLERMRPAFKKDGTVTAGNASGINDGAAALVLMSEKKAKELGIKALATIV
                     NIENAGVAPEIMGIGPAYAIPKVLKRAGLTPEDIGYYEINEAFAAQFLAVNKELKLDM
                     EKVNVNGSGIALGHPVGCTGARILVSLIYEMKRRKDKYGVASLCVGGGPSIATILEVE
                     "
     gene            1472..2737
                     /locus_tag="VFD08_00275"
     CDS             1472..2737
                     /locus_tag="VFD08_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZK09477.1"
                     /translation="MGLIKALTSSVGSGLADQWLETIEPEDTSEGVVFTQGIMLKRGR
                     RGSNKKLTVDAISNGSIIHVPENQCALLVDGGAIVDFTAEPGMFEVKNSSLPSMMSGD
                     LGDSIKETFNRFRFGGDTPLKQQVFYVNLQEMKGIKFGTKNPINYFDDFYNSELFLRA
                     HGTYTIKITDPILFFREVLPKGGGIITMDDIAQMYMSEFLEALQTSINQMSADGIRIS
                     FVTSRSQDLGKYMANVLDEKWTSLRGMDIQSVGIASISYDDDSKELINMRNRGAMLGD
                     ATIREGYVQGTVARGIEAAGSNESGSMGALMGVGMGMQASGGFMGAASGANQAQAERE
                     ALEAQMKAQKEEMERLRAELEGKASEEKPSSPASWKCPNCDTENEGKFCRECGTPKPE
                     KAQGGFCSNCGHKFEGEVPKFCPECGNAN"
     gene            2747..3802
                     /locus_tag="VFD08_00280"
     CDS             2747..3802
                     /locus_tag="VFD08_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09478.1"
                     /translation="MSLLNYKCPSCGASINYDANSDKGLCDFCSSVFTLEELEEYAKK
                     DGASTKEEKSDKQDGAEEEILSYSCNNCGAEVVSDATTSATFCYYCHSPVILTKQLQG
                     VFKPDYIVPFAIDKKKAQETFLAWAKTKSYVPSSFTEASQLEKITGMYLPYWLADAKT
                     QVNVRGIGTKTNVYRRGEVEYTEYEEYPITAQGDFSINKIPELAFTQIDRDLVKSVDD
                     FSSAELKDFNMAYLSGFFSERYDIEKKEAEGALVDQATEYANRFVSSQFSGYDNVKKD
                     VNQIDTELEKVSYVLLPTWVLTYQYLGKNYIYMLNGLNGMAYGELPVDQKKLGLRSGI
                     IFLIIAVLMLLGGRFIW"
     gene            3796..5022
                     /locus_tag="VFD08_00285"
     CDS             3796..5022
                     /locus_tag="VFD08_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZK09479.1"
                     /translation="MVKRSLVILLLMVLLALPSWAVESSIIDEGAFLSAEESQGLQAI
                     SEALQEAFSIQTKTFLLIPDEELEEEDFKALLKESFPFTSERSLYFALDGTRGIAYVG
                     SEGIQELDPDTLQKMLDEALAVDFQGKLILPLTRFYSGIFDILQANPQLAATSESEEA
                     VADEQEVLAPAKVYLDDGANLWNQEQKDMLFKEATRLSNREDMAILLVTTDYNPDKSS
                     EAFIEDLAEEKFGIDTDQVAFLIDMDNRKIEVNTSGRAIDIIHDDRLEAILDGVYLGM
                     EKEDYFLAGQNFIKETDLYLAQGIDPNYGGRKERGENNLSPLDAILGLGLGGVGGFLN
                     FARVKKSYAKKTAPIDFQYRNNIIGGIPLQAGRPINKRVTQRKIPKSGGGDGGSSSTT
                     HKSSSGGTRGGGGKSF"
BASE COUNT         1716 a          895 c         1260 g         1345 t
ORIGIN      
        1 acgtaacccc ttatagcaaa agatttctca atagagtagc tagaagaaaa tcttttaggg
       61 ataatacatt agaaaagagg aagaaattga aaaaagttgt ggtagttgca gcagtacgaa
      121 cccctatagg tgatttttta ggattcttac aaagtgttgg ttcagttgag cttggagaga
      181 tagcagtaaa gggagcacta aaacaagcgg ggatagagcc agaaatggta gaggagctag
      241 cctgtggcat gatttacaag gcgggcaata agggaaatcc agcacgccaa gttcagataa
      301 aaacaggaat gcctgtagaa ggaagtgcct ataccattga tcaacaatgc ggttcaggta
      361 tgaaagcctt tgaacttata caccaatcga ttatgctggg caaaattgat atcggtgtag
      421 ccctaggttc agaaagcatg tcccaggcgc cctacctgtt aaagacactt cgacagggac
      481 aaagaatggg ggatgctaag gccctagatt ccatgctaga agacggatta atatgcgctc
      541 tagaaggata ccatatggga gtaacagcag aaaacctagc agaacgctat gggataagcc
      601 ggcaggagca agatgagctt gcccttctta gtcaaaaaag agcggtgaag gcaatagcag
      661 aaggaaaatt caaggatgaa atagtacctg tagttattca aacacgcaag ggagaaatcc
      721 ttgtagatac tgatgaacat ccaagaaaag acattagtct tgaaaaactt gagagaatga
      781 gacctgcctt taaaaaagac ggaactgtaa cggcgggaaa tgcttcagga atcaatgatg
      841 gagcagcagc gcttgtttta atgtcagaaa aaaaggcaaa ggaactggga attaaagctc
      901 tggcaacaat tgtcaatatt gaaaatgcgg gagtagcccc agaaatcatg ggaatcgggc
      961 cagcttacgc tataccaaaa gtgcttaaaa gggctggact aaccccagaa gatatcggtt
     1021 attatgagat aaatgaagcc tttgctgcgc agtttttggc agtaaataaa gagttgaagc
     1081 tagatatgga aaaagtaaat gtcaatggtt caggaatagc cctaggccac cccgttggct
     1141 gtacaggggc cagaatatta gtttctctaa tatatgaaat gaaaagacga aaagacaagt
     1201 atggagtggc ttcactttgt gttggtggtg gcccatctat tgccacaata ctagaagtag
     1261 aataacaaaa aacaggtatc accaggtaag aaagttatag caaaaggcca tataaggcga
     1321 tttgctagac tatcttttaa aaaaggtaca ataaataaaa acagtaggcc ctattaaaaa
     1381 caacaatagg gcaatattgt tttaaaaaat ataggacaat ttcttggaaa aaaaacaaat
     1441 aaaaaaaggc taaaggtaaa aggaggaagc gatgggacta attaaagcac ttacttcatc
     1501 agtaggaagt gggctggcgg atcaatggtt agaaacaatt gaacctgagg atacatctga
     1561 gggcgttgtt tttacccagg gtataatgtt aaaacgagga agacgaggga gcaacaaaaa
     1621 acttactgtt gatgctattt ctaatggatc tattattcat gtcccagaaa accagtgtgc
     1681 cctccttgtt gatggtggcg ccattgttga ctttacagca gagccgggca tgtttgaagt
     1741 aaagaactct tctcttccct ccatgatgtc gggggatttg ggagatagta ttaaagaaac
     1801 ctttaaccgc tttcgttttg ggggagatac gcctttaaaa cagcaagtct tttatgtcaa
     1861 tctccaggaa atgaaaggca taaagtttgg aacaaaaaac ccaatcaact attttgacga
     1921 tttttataat tcagaactct ttttaagagc tcatggaact tatacaataa agattaccga
     1981 tcccattctc ttttttagag aagtattgcc caagggtggc gggattataa ccatggacga
     2041 tatcgcccaa atgtatatgt cagaattttt agaagccctt caaacctcca tcaaccaaat
     2101 gtctgctgat ggcattcgaa tctcctttgt aacttcaaga agccaggatc taggaaaata
     2161 tatggccaat gttctggatg aaaagtggac aagtcttcga ggcatggaca ttcagtcagt
     2221 agggattgcc agtatctcct atgatgatga ttccaaagaa cttatcaata tgcgtaatcg
     2281 tggtgccatg cttggggatg cgacaattcg cgaaggttat gttcaaggaa cagtggcccg
     2341 tgggattgaa gctgccggca gcaatgagtc tggatccatg ggagccctaa tgggggttgg
     2401 aatgggcatg caggccagtg gtggttttat gggagctgca tcgggagcaa accaggccca
     2461 agcagagcga gaggctctgg aagctcaaat gaaggcacaa aaagaagaaa tggagcgtct
     2521 gcgagccgag ctggaaggaa aggctagcga ggaaaaaccg tcttctcctg ccagttggaa
     2581 atgtcccaat tgcgatacgg aaaatgaggg gaaattctgc agggaatgcg gcacacctaa
     2641 accagaaaaa gcccagggag gtttttgtag caattgtggc cataaatttg agggagaggt
     2701 accaaaattc tgtcctgagt gtgggaatgc aaattaggag gtagaaatgt ctctactcaa
     2761 ctataaatgc ccttcttgtg gtgcttctat aaattatgat gcaaactcag acaagggtct
     2821 ttgtgatttt tgctctagtg tttttacctt agaagagtta gaagagtatg caaaaaaaga
     2881 tggagcaagt acgaaagaag aaaaaagtga taagcaagat ggtgctgagg aagaaatact
     2941 aagctatagt tgcaataact gtggtgcaga ggtagtaagt gatgctacta cctcggcaac
     3001 cttttgctat tattgccata gccctgtaat tttgacaaaa cagctgcaag gggtttttaa
     3061 gcctgattat attgtgccct ttgctattga taagaaaaaa gcacaggaaa catttttagc
     3121 ctgggcaaaa acaaagtcct atgttccaag cagttttact gaagcctccc agcttgagaa
     3181 aattactgga atgtatttgc cctactggtt ggcagatgcc aagacccagg taaatgttag
     3241 aggaatagga acaaaaacca atgtatacag gcggggggaa gtagagtata ccgaatacga
     3301 agaatatcct attacagccc aaggggattt ttctatcaac aagattcctg agctcgcctt
     3361 tactcaaata gatagagatt tggtaaaaag tgtggatgat tttagttcag cagaactcaa
     3421 agatttcaat atggcctatc tttctggatt tttttccgaa cgctatgata ttgaaaaaaa
     3481 agaagcagaa ggcgctcttg tagatcaagc cacagagtat gccaatcgtt ttgtttccag
     3541 tcagttttca ggctatgata atgttaaaaa ggatgtaaat cagattgata cagagctgga
     3601 aaaagtttcc tatgtgcttt taccaacctg ggtgcttacc tatcagtatc tgggaaaaaa
     3661 ctatatctat atgcttaatg gtcttaatgg tatggcctac ggggaacttc ccgttgacca
     3721 gaaaaaattg ggcttacgca gtggaatcat ttttttgatt attgctgtgc ttatgctatt
     3781 aggaggtaga tttatatggt aaagcgtagt ctagtgattc tacttttaat ggtactactt
     3841 gccttgccta gctgggcggt ggaatcctct attattgatg agggagcttt tcttagcgcg
     3901 gaagaaagcc aaggtttaca ggcaatttca gaggcccttc aagaggcttt ttccatccaa
     3961 acaaaaacct ttttattaat tcctgatgag gaattagaag aagaagactt taaagccctc
     4021 cttaaagaaa gctttccctt tactagtgag cgcagccttt attttgcctt agatggaaca
     4081 aggggcattg cttatgtggg aagtgaaggt atacaggagc tagatccaga tactttgcaa
     4141 aagatgctag atgaggcctt ggctgtagat tttcaaggta aactgatttt acctcttaca
     4201 agattttata gtggaatctt tgacatcttg caggcaaatc cccagctagc agctactagt
     4261 gagagtgaag aggcagtggc agatgagcaa gaagttctag caccagcaaa ggtatatctt
     4321 gatgatgggg caaatctttg gaaccaagag caaaaagata tgctctttaa agaagcgaca
     4381 aggctttcta atcgcgaaga tatggccata cttcttgtta caacagatta caacccagat
     4441 aaaagctctg aagcctttat cgaagattta gcagaagaaa agtttggaat agacacagat
     4501 caagtagcct ttcttattga tatggataat agaaaaattg aagtaaatac ttcaggtaga
     4561 gccattgata ttatccatga tgatcgtctt gaagccattc tggatggggt ttatctaggg
     4621 atggaaaaag aggactactt tcttgctggt caaaacttta tcaaagaaac cgacctatac
     4681 ttagcccagg ggattgatcc aaattatgga ggtcgcaagg agcggggaga aaacaactta
     4741 tcccctctag atgctatcct tggcctgggt cttggtgggg tggggggatt tttaaatttt
     4801 gcaagagtga aaaaaagcta tgcgaaaaag acagccccta tagactttca gtatagaaac
     4861 aacattattg gaggcatccc ccttcaagca ggtaggccaa ttaataagcg ggttacacaa
     4921 agaaagattc ctaaatctgg tggtggtgat ggaggaagct caagtaccac tcataaatca
     4981 agctccggag gaacccgggg tggtggagga aaaagtttct agaaaggggc gaaatatttc
     5041 gcctctttta tttatcatcg gattaggcct ataataaaaa ggaggagggg ttatgcaaaa
     5101 ttatgtggat gcatttggaa agcgttttta tctagtggaa aaagaaggag cccttgttta
     5161 ttgtggctac agggaaccaa aaattaaggg cgcatcaacg cccctaatgg atgaag
//
LOCUS       DASRNA010000011         3972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_375650,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000011 DASRNA010000000
VERSION     DASRNA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375650"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFD08_00290"
     CDS             complement(<1..414)
                     /locus_tag="VFD08_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09480.1"
                     /translation="MKKIKNFLALAIKGILYIYTPLIILLFLIYRFFFTSKGADSAYN
                     FIIYGVYALLVGFSYASNYSPKSIEKNLKNFTHVKKVLAKGKWEIVKESTNTMIVKPT
                     FDFPFRLLIDNRVHLEYKDQKVNINGPKYYTDNLLR"
     gene            718..2202
                     /gene="thrC"
                     /locus_tag="VFD08_00295"
     CDS             718..2202
                     /gene="thrC"
                     /locus_tag="VFD08_00295"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZK09481.1"
                     /translation="MKYISTRNKADRVRASEGIVRGLSKNAGLYVPEVLPRMDNINPL
                     LAMNYQELAYEIMSKFFTDFSEKALRESINRAYDKKFAKKEIAPLVEMGQVSFLELFY
                     GATFAFKDMALSILPYLLKEAMKNTQEKRELVILTATSGDTGKAALENFANISGVKII
                     VFFPEKGVSNIQKKSMLTQEGDNAFVVSIKGDFDDAQSGVKEIMNDHNFTQQMEENNY
                     KLSSANSINIGRLIPQIVYYYHSYLTLLRKKEIVEGEKINFVVPTGNFGNILAAYYAK
                     KMGLPIKKLICASNENNALTDFFHTGSYKANRKLELTSSPSMDILISSNLERLLFYLS
                     GGDGDLIKSSMETLDKKKEFQIKGLDLSDFEAYFSTEKEVERAIRDVFKNFDYLMDPH
                     TAVAYSAYKKYKREKKDKTKTVIVATASPFKFGEKVASSIGIDTKDKDDFTIIEELGL
                     RANLPIPKNILDLRDKAILHKQNCQKEGIEGMILDILGVGNERD"
     gene            2195..3085
                     /gene="thrB"
                     /locus_tag="VFD08_00300"
     CDS             2195..3085
                     /gene="thrB"
                     /locus_tag="VFD08_00300"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZK09482.1"
                     /translation="MIKVKVPASSANIGPGFDSIGMALNLYNIFSFEEIDRGLEIIGS
                     LEDKKAKDHLVYSSMVKVFERVGYFPKGIKIKIEASIPISRGLGSSASCILAGVIGAN
                     KMAGSPLTQEEIFKIATEIEGHPDNIAPALFGGFVSSLMEGEKIYYNKIDISKGLKFI
                     AIIPDFTLSTRRAREVLPRVISHREAVENVSRLALLISALSNGRFDLLRPALKDKLHQ
                     PYRGELIEGFTALMDKIYDLGALGGYLSGAGPTIMAMIKEEDFGFIKEIKAYLESISL
                     GWDVKELEMDLEGAQIVEKP"
     gene            3705..>3972
                     /locus_tag="VFD08_00305"
     CDS             3705..>3972
                     /locus_tag="VFD08_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09483.1"
                     /translation="MKTKRWVALMLIVLLVLTVSACGGESADADEAEEVTETDEAEEA
                     EEAEEVEETTEAEESTGSTLVFDYRDEGEVFFDYPASYSHSDSTG"
BASE COUNT         1381 a          611 c          824 g         1156 t
ORIGIN      
        1 cctaaggaga ttgtcggtat aatatttggg accatttata ttaacttttt gatctttata
       61 ctcaagatgg accctgttat caatcagtag tctaaaggga aaatcaaatg taggctttac
      121 aatcattgta tttgtagact cttttacgat ctcccatttt cccttggcta ggactttttt
      181 tacatgggtg aagttcttaa ggtttttctc tattgattta ggagaataat ttgaagcata
      241 ggaaaatcct accaagaggg cgtagactcc ataaataatg aagttatagg cagagtcggc
      301 ccccttgctt gtaaaaaaga acctatagat aagaaaaagc aatataataa ggggagtata
      361 gatatataaa atccccttga tggccagggc tagaaagttc tttatctttt tcacttaact
      421 tctcctcaaa cttataaaac gatgtactct tcttagaata ccagttactg ttattgttct
      481 tactttacca taagtacttt tcataggcaa aattaacgtt ctctagtttt tacataggcc
      541 ctagcttgcc ttggctaatt agtgaaaaaa gaggtgtctt atgttctatt attaacttat
      601 aaggatatga taggatacag gtatagaagg aaaaaaacac ctctacttaa aaagagcact
      661 ttatttagta gggttttttc taaaacttta gcctatagcc ttaattagag gagtcaaatg
      721 aaatatataa gcaccagaaa taaagcagac cgtgtaaggg caagtgaagg gatagtaaga
      781 ggtctatcaa aaaacgcagg gctctatgtg cctgaagttt tgccaaggat ggataatatc
      841 aaccctttac ttgctatgaa ctatcaggag ctagcctatg agataatgag caagtttttt
      901 actgacttta gtgaaaaagc actgagggag tctataaata gggcctatga taagaagttt
      961 gcaaaaaaag aaatagctcc actagtagag atgggacaag tatcattttt agaactattt
     1021 tatggtgcaa cctttgcttt taaagatatg gccctatcta ttttgcccta tcttttaaaa
     1081 gaagccatga aaaacaccca agaaaaaagg gaacttgtga tcctaaccgc tacttctggc
     1141 gatacaggaa aagctgccct agaaaacttt gccaatatta gtggtgttaa gataatcgta
     1201 ttttttcctg aaaaaggagt tagcaatatt caaaaaaaat cgatgttaac acaagaagga
     1261 gacaatgcct ttgttgtatc cataaaggga gattttgatg atgctcaaag tggggtgaaa
     1321 gaaattatga atgaccataa ctttacccag caaatggagg aaaataatta caagctttct
     1381 tccgcgaatt caattaatat aggcagatta atccctcaaa tagtatatta ctaccatagt
     1441 tatctaactc ttttaagaaa aaaagaaatt gtggaagggg agaagataaa ctttgtagtg
     1501 ccaactggca actttggaaa tatactagct gcatactatg ctaaaaagat gggtcttccc
     1561 ataaaaaaac taatctgtgc ctccaatgaa aataatgccc taacagactt ttttcacact
     1621 ggaagctata aggccaatag aaaacttgag ttaacctctt caccttctat ggatattttg
     1681 atttcttcta atttagaaag acttttattt tatttaagtg gcggagatgg ggatttaata
     1741 aagagttcca tggaaacctt agataaaaaa aaggagtttc agataaaggg gcttgacctt
     1801 tcagattttg aggcgtattt ttccacagaa aaggaagtgg aaagggctat tagggatgtc
     1861 tttaaaaact ttgattatct tatggacccc catacagctg tagcctatag tgcctataaa
     1921 aaatacaaaa gagaaaaaaa agacaagaca aagacagtta tagtagctac cgcaagcccc
     1981 tttaagtttg gagaaaaagt ggctagctca ataggtatag atacaaagga taaggatgat
     2041 ttcaccatta tagaagaatt ggggttaagg gcaaatttac ctatacctaa aaacatttta
     2101 gatcttaggg ataaggctat ccttcataag caaaactgcc aaaaagaagg catagaaggt
     2161 atgattttag atatattagg agtgggcaat gagcgtgatt aaagtgaaag ttccagctag
     2221 cagcgcaaat ataggtccgg gatttgatag tattggaatg gctttaaacc tctataacat
     2281 cttttccttt gaggaaattg ataggggcct ggaaataata ggatcattag aggataaaaa
     2341 ggccaaagac catttggtat atagctcaat ggtaaaggtc tttgaaaggg tgggctattt
     2401 tcctaaagga ataaaaatca agatagaagc tagtatcccc atatctagag gtcttggcag
     2461 cagtgcatct tgtatcctag caggggtaat aggggccaat aagatggccg gatcaccctt
     2521 gacccaggaa gaaatattta agattgcaac agagattgaa ggtcatccag acaatattgc
     2581 gccagcctta tttggagggt ttgtttcttc tcttatggag ggagaaaaaa tatattacaa
     2641 taaaatagat ataagcaagg gactaaaatt tatcgccata ataccagatt ttactttatc
     2701 aacaagacgg gctagagaag tcttgccaag ggtgatttcc catagggaag ccgtggaaaa
     2761 tgtaagtagg cttgctttac ttatatcggc cctttcaaat ggaagatttg atttattaag
     2821 gccagcacta aaagacaaac tgcaccaacc ctatagagga gagttaatag agggatttac
     2881 tgctctaatg gacaaaatct atgatcttgg cgccctgggt ggatacctaa gcggagcagg
     2941 ccctactatt atggcaatga taaaagaaga agattttggt tttataaaag aaataaaagc
     3001 ttaccttgag tctataagtc ttggctggga tgtaaaggaa ctagaaatgg acttagaggg
     3061 agcacaaata gtagagaagc cctagcttct ctactatttt tctgtatcca ttgattctgg
     3121 atgggcttta ttgaaaaacg ctcctctttt ttttatggta tgcctccttt aaggatgaca
     3181 attatttggc tcttgggctg aagggtggct acctatagaa aatggaggta aagacattgc
     3241 ttctaattaa taacattttt tggaaaatac ccctggcccc ttgctaaatt aactaaatat
     3301 agtataataa aagcatatac cttgttaagc aaaatacata gattggattt aaggtgagtg
     3361 atatagcaca aatgtctaaa tctatacaat tacgaggtat attttgtaat aatagaaaga
     3421 acttcaaaaa ggcagtattt tgccagatgt ctaagaaatg aatattggag gaattattag
     3481 gtgagacaaa tgacaataaa gcgctttgaa tattagtcca tgactggcca ggctgctatg
     3541 ctcactctag gaaaaaagag acgggagttg ttttacttac tattaatagc aaagagttag
     3601 agttaaaaga tgctgggcct aatggtattt atattttggg ttttatgaac ataggcatct
     3661 tatgataaaa gtgtttgaaa aaaaataaaa aaagggagat tattatgaaa acaaaaagat
     3721 gggtagcact tatgctcatt gttctgctag tactaacagt atcagcctgt ggcggtgaaa
     3781 gtgcagatgc ggatgaagct gaggaagtta cagaaactga cgaagctgaa gaagctgagg
     3841 aagctgaaga agttgaagaa actacagaag ctgaggaatc cactggatca actctagtat
     3901 ttgattatag agatgaagga gaagtgtttt ttgactatcc tgcctcctac tcccacagtg
     3961 atagcactgg ta
//
LOCUS       DASRNA010000012         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_600071,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000012 DASRNA010000000
VERSION     DASRNA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600071"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..>2141)
                     /locus_tag="VFD08_00310"
     CDS             complement(186..>2141)
                     /locus_tag="VFD08_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HZK09484.1"
                     /translation="ALYRESIMDRETADAMAHAMKNWAIEKGASHYSHWFIPLTGLSA
                     EKHNSFISKEDELPLLRFSGSSLIKGETDGSSFPTGGLRATFEARGFTYWDISSFAFI
                     RGHVLYIPSIFLSYKGDQLDLKSPLLRAVDSFNQRATELLHLLGEEKVKGVKPMIGLE
                     QEYFLVKKEYFLQRPDLFYTGRTLIGSPPPKGQEFEDHYLGSIPTNVSKFMQDVNMEL
                     WRLGIFAQCEHNESAPSQFEIVSHQEIASLSIDQNIILMDILKEKAKEHGFVCLLHEK
                     PYAGINGSGKHNNISFLTSEGINLLEPGDSPKENLRFLLFLGAFILATEKHATLLRLA
                     SSDPGNDERLGGFEAPPAIVSIYLGEHLHASLGEFFGEKTYSTNQKKQSLPQLADLPS
                     DSTDRNRTSPIAFTGNKFEVRMLGSSRSAAMLNICLLAALAEELAQITRDLEKMACPE
                     KEAYAYLTKKLSAVKHILYEGDNYSSLWYKKAQKRGLPILDNYVACIASLLSQKSVDM
                     FSRSGVLSKKELEARSHILYTQYSHTLMAEAKTIQLMLNKDLLPALRKEILLRKNLSH
                     IDPYFEKELNDALNLYKKTTYKNKELSIYLDQALKEGSEEKKALFFSTRVRSAIKELR
                     SYTDQMEKLLPGDSYPYPDYGKMLYGL"
BASE COUNT          522 a          439 c          543 g          637 t
ORIGIN      
        1 tgcttctacc ccaacaggag cccaggataa acctcttgag ccggtagtga ttgagcgcat
       61 tgatattata gaggactagt tttctctaac aagtaggcag gttgattggc agaataatca
      121 gcctgttttt ttcttgtcaa aaaaagaatc atcctgccag cttagccagc aagatgattt
      181 agtctttaga gtccatagag catttttcca taatcaggat agggatagct atctccagga
      241 aggagctttt ccatttggtc tgtgtagctt cttagctctt ttatagcact tcgcacccta
      301 gtagaaaaga agagggcttt tttctcttca gagccttctt taagggcctg gtcaagatag
      361 atgctgagtt ccttgttttt ataggttgtt ttcttgtata gatttagggc gtcattgagt
      421 tctttttcaa agtagggatc gatatgactt aggttttttc gcagtagaat ttcttttctt
      481 agggcgggca ggaggtcttt atttagcata agctgtattg ttttggcctc tgccatcagg
      541 gtgtggctgt actgggtgta gaggatatgg cttctagcct cgagttcttt tttggagaga
      601 accccacttc tactaaacat gtccacgctc ttttgtgaga gcagggaggc gatgcaggca
      661 acataattat ccaggatggg aaggcctctt ttttgggcct tcttatacca gaggctgcta
      721 tagttatcgc cctcataaag tatatgctta acggcggaga gtttttttgt taaataggcg
      781 taggcttctt tttctggaca ggccattttt tctaggtccc tggtaatctg ggccaattcc
      841 tctgccaggg cggcaagcag gcagatattt agcatggcgg ccgagcggga ggatcctagc
      901 atgcgcacct caaatttatt gcctgtaaag gcaatgggag aagtgcgatt tcggtcggta
      961 ctatccgagg gaagatcggc cagctggggc agggattgtt ttttctgatt ggtagaatag
     1021 gtcttttctc caaaaaactc tcccaggctg gcatggaggt gttctcctag atagatggac
     1081 acaatggcag ggggggcttc aaagccaccc aggcgctcat catttccagg atcactgctg
     1141 gccaggcgca ggagggtggc gtgtttttct gtggccagga taaaggcccc taaaaagagg
     1201 agaaaacgca ggttttcttt gggggaatca ccgggctcta aaagattaat cccttcactg
     1261 gttaaaaaag aaatattatt atgtttgccg ctgccattga ttccggcata gggtttttca
     1321 tggagcaggc agacaaagcc gtgttctttg gccttttctt taagaatgtc cataagtata
     1381 atgttttggt cgatagatag gctggcaatc tcctgatggc ttacaatttc aaactgagag
     1441 ggggctgatt cattgtgctc gcactgggca aagataccaa gacgccataa ttccatattt
     1501 acatcctgca taaatttact tacatttgta ggtatactgc ccagataatg atcttcaaat
     1561 tcctggccct tgggcggcgg ggatccgatt agggtcctgc cggtgtaaaa aagatcgggt
     1621 ctttgtagaa agtattcttt tttcactaga aagtactctt gctctaggcc gatcatgggc
     1681 ttgacgccct ttactttttc ttcgcctagc aggtgtaaaa gctctgttgc cctttgatta
     1741 aaagagtcaa cagcccgtag aaggggactt ttaaggtcca actgatctcc cttataggaa
     1801 agaaagatac tgggaatata aagaacatgg cccctgataa aggcaaagga gctaatatcc
     1861 caataggtaa agccccgggc ttcaaaggtg gcccgcaggc ctcctgtggg aaaactactg
     1921 ccatcggttt ctcccttgat caaggaagaa ccagaaaagc gaagtagggg gagctcatct
     1981 tcttttgaga taaagctatt gtgtttttct gcactgagtc ccgtaagggg gataaaccag
     2041 tggctatagt ggctggcgcc tttttcaatg gcccagtttt tcattgcatg ggccatggcg
     2101 tcggcggttt ctctgtccat aatgctttcc ctatagaggg c
//
LOCUS       DASRNA010000013         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_376970,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000013 DASRNA010000000
VERSION     DASRNA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376970"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..189)
                     /locus_tag="VFD08_00315"
     CDS             complement(22..189)
                     /locus_tag="VFD08_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09485.1"
                     /translation="MEELRNCKSLDGAIMRANSRRKKHKDKKKKTIRARLKSTIKEVK
                     KKFAENTAGKA"
     gene            complement(219..1334)
                     /gene="tgt"
                     /locus_tag="VFD08_00320"
     CDS             complement(219..1334)
                     /gene="tgt"
                     /locus_tag="VFD08_00320"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZK09486.1"
                     /translation="MFKFELEHVDKYTGARAGKFHTPHGIIETPVFMPVGTQAAVKAV
                     STDSLKDMGTQVLLSNTYHLNLRPGSEIIKSAGGLHKFMNWDKAILTDSGGFQVFSLK
                     GLRKIREEGVEFQSHLDGSKIFMSPESSIQIQENLGADIIMAFDECAPALSSRDYIVP
                     SMERTLRWLERCKEAKTRDDQALFGIIQGGLFKDLRIQSAIKTCQIDLPGYAIGGLSV
                     GETREEMLEILDILGPYLPKDKPRYLMGVGTPQDFVEGILRGIDMMDCVHPTRIARHG
                     LAMTSRGMISIKRQEFTADLSPLDPNCSCPCCRDYSKAYLRHLYKSGEILSSMLMSIH
                     NLSFLIGLAQDLRQAIFNDESENIRKKLYTVMGYAKS"
     gene            complement(1327..2349)
                     /gene="queA"
                     /locus_tag="VFD08_00325"
     CDS             complement(1327..2349)
                     /gene="queA"
                     /locus_tag="VFD08_00325"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZK09487.1"
                     /translation="MKTSDFYYDLPKELIAQSPLQKRSESRLLLLGRKSARIKEGHFY
                     DIVDYLHPGDVLVLNETKVLPARVYGSKTTGARVEFLFLERLNEKEWSVLVKPGRKAR
                     PGDRFVLSKKLSLEIKDILDDGLRRVELFYEGIFEEVLAEIGTMPLPPYIHESLEDQN
                     RYQTIYAKAMGSSAAPTAGLHFTQELLERIENKGIKIARLTLHVGLGTFRPVKSDNLK
                     EHIMHEESYLLDEENARLIRSADRVIAVGTTSVRTLESIYLKYGSIQADQDRTSIFIY
                     PGFTYHVIDALITNFHLPSSTLLMLVSAFSTRENILKSYNYAIQEKFRFFSFGDAMMI
                     TEELDV"
     gene            complement(2346..3371)
                     /gene="ruvB"
                     /locus_tag="VFD08_00330"
     CDS             complement(2346..3371)
                     /gene="ruvB"
                     /locus_tag="VFD08_00330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006039849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZK09488.1"
                     /translation="MNKRIITPEILQEENKELSLRPRHLEEYIGQTKNVEQLKVFIEA
                     AKIREEHLDHVLLYGPPGLGKTTLATIIANELGVGIKVTSGPAIERPGDLAAILSGLK
                     ENDVFFIDEIHRLNSAVEEVLYPAMEDYALDILIGKGPSARSVRLDLAPFTLVGATTR
                     AGDLSSPLRDRFGVLMRLELYSQEELEMILKRSAKVLGVELTASSRKEIARRSRGTPR
                     ITNRLLKRVSDYALVNNNGIISEEVACQALHLLEVDELGLDKTDMNLIRIMATLFDGG
                     PVGLDNLAASSGEESSTIEDVYEPYLMQIGFIKRTARGRVLLPRAYEHLGLPYEGEGP
                     LFDEVQW"
     gene            complement(3375..3950)
                     /gene="ruvA"
                     /locus_tag="VFD08_00335"
     CDS             complement(3375..3950)
                     /gene="ruvA"
                     /locus_tag="VFD08_00335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZK09489.1"
                     /translation="MLSFIRGEIAYIQTDHVVIDHGGLGIKIFLSQRDLSELTMEEVM
                     IYTELVVREDDLSLYGFIQPLDREIFQRLQKVSGIGVKTALGILSIFSGREIINFILE
                     ENLPLLTKAPGVGKKTASRMILELKDHFSKEYSFMEIESDEGQETKQELREALLSLGY
                     GGGEIHRAISQLDLSKSIEVLLREALALLVK"
     gene            4128..5351
                     /locus_tag="VFD08_00340"
     CDS             4128..5351
                     /locus_tag="VFD08_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09490.1"
                     /translation="MKALGLFLLLFIGFVLLFYYIFLVITGKRLAKLVNSKDRFIWNG
                     LRNITDKSSRGRATMFLDESQLVISSLGLTQIYSKLQQGSLDDGFSIPFTDIAVFDYS
                     FQSKVPFLRRIINQIFKINNYTLKISYFDDLGQIVPLKFKASSLDPLDFEATFADFNN
                     KIYGDHGLRRSTDNEIIPLSQRMGIDDTAPLSPAFNEDPGDKTVLLKEDTPFDREEKT
                     TILPKESFLEEDIRNNDIFEEAENKTTILSKESFLEEDIRNNDIFEETENKTTILAKE
                     TFLEEETPRKSEIFEEEKDHDLEKTVFLSGNFLKRKEEKDFPKEDKTVLSPPIVKEED
                     QVEQKIQRRSFLDRSPRKGPEEGLEDKTISLELPDELKKKENIGMSEEEIKQEELERL
                     KKLKEFFSERNEDQG"
     gene            complement(5362..6000)
                     /locus_tag="VFD08_00345"
     CDS             complement(5362..6000)
                     /locus_tag="VFD08_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HZK09491.1"
                     /translation="MKLIRKDSIIQFVEKRSQFIGYAFYVESKEESEEILTKLWKEHR
                     KCTHICTASHIGLSEKRGEFDDNGEPSLTAGYPMLQILETEDIRQVLICAVRYFGGIK
                     LGKGGLIRAYTKTAQLSLEAAGLKEVREAQKWHLSYDYSHHGSVEYILQEEKVLGLEP
                     IYTSNVQREIYIEKAELCKKLMDISDGKIDFTYKGKYYLDPTKKNMELEKIK"
     gene            complement(5987..>6173)
                     /locus_tag="VFD08_00350"
     CDS             complement(5987..>6173)
                     /locus_tag="VFD08_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09492.1"
                     /translation="FSHRHMCSFLFPFTQGAALSAFLENATPIEKEYKEEGTWILARV
                     NKRQREEFREYICHEIN"
BASE COUNT         1807 a         1334 c         1089 g         1943 t
ORIGIN      
        1 acttttatct ataaaaaact cttatgcttt gccagccgtg ttttctgcga atttcttttt
       61 cacttctttt atggtggatt taagcctagc tctgattgtt tttttctttt tgtctttatg
      121 tttttttctt ctgctattgg cccgcataat tgccccatca agggacttac aatttcttaa
      181 ttcttccatc cttactcttt tctaccaatt attcagcact agctcttagc atagcccatt
      241 acggtataaa gcttttttct tatattttca gattcgtcat taaaaatggc ttgtcgcaag
      301 tcctgggcaa gcccaataag aaagctaaga ttgtgaatgc tcattagcat tgaacttaag
      361 atttcacctg atttataaag gtgacgtaaa taagccttac tatagtccct acaacaaggg
      421 caagagcagt taggatcaag aggagaaaga tctgctgtaa attcctgtct ttttatcgaa
      481 atcatccctc tagaagtcat tgcaagacca tgcctagcaa ttcttgtggg atgaacacag
      541 tccatcatat caatccctct tagaattcct tctacaaaat cttgaggggt acctactccc
      601 ataaggtatc gtggtttatc cttgggaaga tagggaccaa gaatatctaa aatctccagc
      661 atttcttctc tggtctctcc tacacttaaa cccccaatag cgtagccagg cagatcaatt
      721 tggcaggttt ttattgctga ttggatacgt agatctttaa aaagtcctcc ctggataatg
      781 ccaaaaagtg cttgatcatc tcgtgtttta gcctctttac aacgctccag ccagcgtagg
      841 gtcctctcca tactaggaac gatatagtcc cttgagctta aagcaggtgc acattcatca
      901 aaagccataa taatatcagc accaagattt tcttgaattt gtattgaact ttctgggctc
      961 ataaaaatct ttgatccatc cagatggctt tgaaactcaa cgccctcttc tcttattttt
     1021 cttaggcctt ttaaactaaa gacctggaag cccccgctat cggttaagat tgccttatcc
     1081 caattcataa acttatgcag gcctccagct gattttataa tctcactacc cggtctaaga
     1141 ttaagatggt aggtattgct aagaagcacc tgagttccca tgtcttttag ggaatccgta
     1201 cttacggcct ttaccgctgc ctgggtacca acaggcataa aaactggtgt ctcaataatg
     1261 ccatgggggg tatgaaactt ccctgccctg gcaccagtat atttatccac atgttcaagt
     1321 tcaaatttaa acatctagtt cctccgtaat catcatggca tcgccaaagc taaaaaaccg
     1381 aaatttttct tgtatggcat aattatagct ctttaaaata ttttctcttg ttgaaaaagc
     1441 actaactaac ataagaaggg ttgagcttgg aaggtgaaaa ttagtaatta gcgcatctat
     1501 aacatgatag gtaaatccag gataaataaa aatagatgtc ctatcctggt ctgcttggat
     1561 agagccatat tttaaataaa tactctccag ggttcttaca ctagtagtcc ctaccgcgat
     1621 aactcggtca gcactgcgaa taagcctggc attttcttca tcaagtagat agctttcttc
     1681 atgcataata tgctctttaa ggttatcgct cttaacaggt ctaaaggtcc ctagtcctac
     1741 atgcagggta aggcgtgcaa ttttgattcc tttattttca attctttcta aaagctcctg
     1801 ggtaaaatga aggcctgctg ttggcgcagc actagaaccc atggctttag cataaatagt
     1861 ctggtagcga ttttgatctt ctaaactttc atgaatatag ggtggcagag gcatagtgcc
     1921 aatttctgct aggacttctt caaatattcc ctcataaaaa agttctaccc ttcgaaggcc
     1981 atcatcgaga atgtccttta tctcaagact taacttctta gaaagaacaa aacggtctcc
     2041 cggcctagcc ttgcgtccgg gttttaccag gacgctccac tctttttcat ttagccgttc
     2101 tagaaaaaga aattctaccc gggcgccagt ggttttacta ccatagactc ttgcaggtag
     2161 taccttggtt tcgtttagga caagcacgtc tcctggatga agataatcca caatatcata
     2221 aaaatgcccc tcttttatcc tggcgctctt tcgtccaagt aagaggaggc ggctttcact
     2281 tcgtttttgc aagggcgact gtgctattag ttccttgggt aaatcatagt agaagtctga
     2341 tgttttcacc actgcacctc atcaaaaagc ggcccttctc cctcataggg aaggcctaaa
     2401 tgttcatagg cacgcggaag aagcaccctt ccccgggctg tgcgttttat aaaaccaatc
     2461 tgcattaaat agggctcata aacatcttca attgttgaac tttcttctcc tgaggatgca
     2521 gccagattat ccaggcccac cgggccccca tcaaaaaggg tggccataat ccgaatcaag
     2581 ttcatatctg tcttatccag gcctagctca tcaacttcca gtaaatgaag ggcttggcat
     2641 gccacttctt cactgattat gccattatta tttactagag cataatcact cactcgcttg
     2701 agcaaacgat tggtaatcct aggagtacca cggcttcttc gagcaatttc ttttcgtgag
     2761 gaagctgtaa gttcaacccc aaggaccttg gcactgcgct taagaatcat ctctagttcc
     2821 tcttggctat atagctccag gcgcattagg acaccaaaac gatctcttag gggactactt
     2881 agatctcctg ctctggtggt agcccctaca agggtaaagg gagccaggtc caaacgaaca
     2941 ctccgagccg aaggcccttt tcctataaga atatctaagg cataatcttc catggcagga
     3001 taaagtactt cttccacagc agaattcaat ctatggattt cgtcaataaa aaaaacatca
     3061 ttttccttta gtccagacaa gatagcagcc aaatctcccg gtcgctcaat ggcaggacca
     3121 cttgtgactt tgattcccac tcccagttca ttagcaataa ttgttgctag ggtagtctta
     3181 ccaagtcctg gtgggccata aagtagcaca tggtctagat gttcttcccg aatctttgca
     3241 gcttctataa ataccttaag ttgttctaca tttttagttt gaccgatata ttcttccaag
     3301 tggcgaggcc taaggctcag ttctttgttt tcttcttgaa gaatttccgg tgtgataata
     3361 cgtttattca tcatttattt caccaatagg gccagggcct cacgcaataa aacttcaatg
     3421 gacttagaaa gatcaagctg actaatcgct ctatggatct cccctcctcc atagcctaag
     3481 ctaagaagtg cttctcgtaa ttcctgtttt gtttcttgtc cctcatcact ttctatttcc
     3541 ataaaactgt attctttaga gaagtgatcc ttaagttcaa ggatcatacg agatgctgtt
     3601 ttttttccca cccctggagc tttagttagg aggggaaggt tttcttccaa aataaaattt
     3661 ataatctctc gtccactaaa aatacttagt atacccagtg ctgttttaac accaattccg
     3721 cttacctttt ggagtcgctg aaatatttct cgatccaagg gttgaataaa gccatatagg
     3781 cttaggtcat cttctcgaac aactagctca gtatagatca taacttcttc catcgttagc
     3841 tcactaagat ctctttgact taaaaaaatc ttaattccca gccctccatg gtctataaca
     3901 acatgatctg tttgtatata tgcaatttct cctcgtataa acgataacat aatggaatgt
     3961 cctttaaaat ttgcgggcaa agccctagaa tgtggttttc tttacttcct aaagtatacc
     4021 caatgaacga tataccgtca acaaaggaca cttatacttc caaataaaag gactttcttt
     4081 ttgcttcttt ttaagttata ctcagttgtg aaagataagg aggatttatg aaagcacttg
     4141 gacttttttt attgctgttt atcggctttg ttttattgtt ttactatatt ttcctagtta
     4201 ttacaggcaa gcgactagcc aaactagtaa atagcaagga ccgttttatt tggaacggct
     4261 tacgtaatat tacagataaa agttctagag gaagggctac tatgttttta gatgagagtc
     4321 aacttgtcat ctcttccctt ggcctgactc agatttactc aaaacttcaa caaggaagcc
     4381 ttgatgatgg cttttctatt ccttttacag atattgccgt ttttgactat agttttcaaa
     4441 gcaaggtccc ttttctgcga cggattatca atcaaatctt taagataaat aattatacat
     4501 taaagatttc atactttgac gatttgggac aaattgtccc cttaaagttc aaagcatcta
     4561 gtctggatcc tttagatttt gaagccactt ttgctgactt taacaacaaa atctatggtg
     4621 atcatggtct tcgccgcagt acagacaatg aaatcattcc tctttctcaa agaatgggca
     4681 ttgatgacac cgccccccta tctcctgctt ttaatgaaga ccctggcgat aaaactgtat
     4741 tattaaagga agatactccc tttgataggg aggaaaaaac aaccatcctg cctaaggaaa
     4801 gcttcctaga agaggatata agaaataatg atatttttga agaagcagaa aataaaacaa
     4861 ccatcctgtc taaggaaagc ttcttagaag aagatataag aaataatgac atttttgaag
     4921 aaacagaaaa taaaacaacc atccttgcca aggaaacttt tcttgaagaa gaaactccaa
     4981 gaaaaagcga aatctttgaa gaagaaaaag accatgactt agaaaaaact gttttccttt
     5041 caggaaattt cttaaagaga aaagaagaaa aagattttcc taaagaagat aagactgtat
     5101 tatcccctcc tatcgtaaaa gaagaagacc aagttgaaca aaagattcaa aggcgctcct
     5161 ttcttgatag atctcctcga aagggtcccg aagaaggcct tgaggataaa acaatttcac
     5221 tagaattacc cgatgaactc aagaaaaaag aaaatattgg aatgagtgaa gaagaaatta
     5281 aacaagaaga gctcgaaaga ctaaaaaaat taaaagaatt cttttctgaa agaaatgaag
     5341 atcagggata aggcctgatc ttcattttat tttctctagc tccatatttt ttttcgttgg
     5401 gtccaaatag tatttgcctt tataagtgaa atcgatcttt ccatcactga tgtccattag
     5461 cttcttacag agctcggcct tttctatata gatttctctt tgcacattag aggtataaat
     5521 aggttctaga cctaagactt tctcttcttg taaaatatac tccactgaac catgatgtga
     5581 ataatcatag ctaagatgcc acttttgcgc ctctcttacc tcttttagac cagctgcctc
     5641 taaacttagc tgggctgttt ttgtataggc gcgaatcaaa ccgcccttac ccaacttgat
     5701 tcccccaaaa tatcgaacag cacaaattaa tacttgcctt atatcctctg tctctagtat
     5761 ttgaagcata ggatagcccg ctgttagact gggctctcca ttatcatcaa attctcctct
     5821 tttttcgcta agtccaatat ggctagcagt gcagatatga gtgcattttc gatgttcttt
     5881 ccatagtttt gtaagaatct cttcgctttc ttccttggac tctacataaa aagcatagcc
     5941 aataaattga ctcctttttt caacaaattg gataatagag tcttttctaa ttaatttcat
     6001 ggcagatata ctctctaaat tcttcccttt gacgtttatt aacccgggcc aggatccagg
     6061 tcccttcttc cttatattct ttttctatag gggtggcatt ttctaaaaag gctgataggg
     6121 ctgctccttg ggtaaaggga aaaagaaaac tacacatatg gcgatgagaa aag
//
LOCUS       DASRNA010000014         3612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_226750,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000014 DASRNA010000000
VERSION     DASRNA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226750"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VFD08_00355"
     CDS             complement(<1..1218)
                     /locus_tag="VFD08_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK09493.1"
                     /translation="MKPIKLEMNAFGPYAQKVEIDFSKFYQGGLFLISGPTGSGKTTI
                     FDAISFALFGEASNKKRESHTLKSHYTQEELCYVNFLFELGGKKIEIYRAPTQEAQGK
                     RKALVTYSSTAELHIDDIPVAKGVMEVNEKIHEILGLNYEEFSQIILLPQGEFQKLLE
                     ASSADKEKIFRRIFSTQKLQAFTQALREEERRLDSDLKTNQTRLGEVLGPSYPKTSAN
                     ELIKGLEEAIESKKPLLEELAKKVSEEEKKKSKLEIVLHQTKEKLELFQKRKIHLLEE
                     ASVEEDKKIIKINHRAKELLPYRQSLLEGEKSLDFYQKKRKEDQSRYLDSETKKKLVK
                     DSFTKAEEERKNIAKLQKRLEELIGIKEGLGLYLGAKKEKEEFEKKAQELAKEIKNLE
                     KKEKEEEDKKAAYE"
     gene            complement(1205..2302)
                     /locus_tag="VFD08_00360"
     CDS             complement(1205..2302)
                     /locus_tag="VFD08_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HZK09494.1"
                     /translation="MKILHTADLHLGKSLNERSLIDLQKEMLEGLIEAVKKHKIELVL
                     LAGDIYDRSLPPKEAVELLSLFLSKLCREMGVKVVMIPGNHDSSERIAYAADLLWGMG
                     LYIGLVGKEITKIEIDDVDIYGLPYVDQFKLNYFHEKRFANLEEAMAHHLSSISLDPD
                     KFNIMMTHHYVLGKNPLLESDSERPLFLGKTENLSTALFNQFDYVALGHIHTAQQVAD
                     NIYYSGSPMKYSKSEAGKTPGYYVIDTQKNLVDFHALSLSKDIRVFTGFFNDLMQGSS
                     PDLSYFELEDRSYIPDAMNRLRQSYPEALGLSYTKMESNLITKSGEDLGRLDHLSLFK
                     SFYLESTGNELTKEESKIVVEMLERSSRETD"
     gene            2457..3383
                     /gene="pyrB"
                     /locus_tag="VFD08_00365"
     CDS             2457..3383
                     /gene="pyrB"
                     /locus_tag="VFD08_00365"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZK09495.1"
                     /translation="MLKGRHLINSEDFSTEEIQSIISLAQTIIEGPQRFSHSCDGKLL
                     GTLFFEPSTRTRLSFESAMLRLGGRVIGFADEASSSSTKGESLIDTVNTVASYADILA
                     MRHSKEGSSYLASAKCPVPLINAGDGGHQHPTQTLADLLTITETKGKTENLVVGFCGD
                     LKFGRTVHSLVKVMSRFPSNDFVFISPKELHIPNHLKLGLERSGVSFIEVERLEEVID
                     KLDVLYMTRVQKERFFNEADYMRLKDSYILDKNKLKSAKEDLAILHPLPRVNEIHPEV
                     DDDPRALYFKQVRYGMYVRMALILKVLGVEEC"
     gene            3377..>3612
                     /locus_tag="VFD08_00370"
     CDS             3377..>3612
                     /locus_tag="VFD08_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HZK09496.1"
                     /translation="MLNINSIAKGIVLDHITPGKGYEIFKLLELDKASYTVALIMNAQ
                     SKKHGRKDIVKIENVIDMDLRVLGILDEDITINI"
BASE COUNT         1004 a          749 c          655 g         1204 t
ORIGIN      
        1 ctcataggct gcttttttgt cttcctcttc tttttccttc ttttcaaggt ttttgatttc
       61 tttagctagc tcctgggctt ttttttcaaa ttcctctttt tcttttttgg cacctaaata
      121 taaaccgagt ccttctttta ttccaattaa ctcttctagt cgtttttgga gcttggctat
      181 attttttctt tcttcttctg cctttgtaaa actatctttt accagctttt ttttcgtctc
      241 actatcaaga taccggcttt gatcttcctt acgctttttt tggtaaaaat ccaggctttt
      301 ttctccctcc aacaagctct gcctataggg caagagttct tttgctcgat ggtttatctt
      361 tataattttc ttatcttctt ctacagaagc ttcctccaga agatggatct ttcttttttg
      421 gaaaagctct agtttttcct ttgtttgatg aagcacaatt tctaattttg attttttctt
      481 ttcttcctca ctcaccttct ttgccagttc ttctaagagg ggctttttac tttcaatagc
      541 ttcttctaag cccttgatta gctcattagc actggtcttt ggataagatg gtcccagaac
      601 ttcgcctaat cttgtttgat ttgttttaag atccgagtcc agtcttcttt cctcttctct
      661 aagagcctgg gtaaaggctt ggagtttttg cgtggaaaaa attctccgaa aaatcttttc
      721 cttatccgca ctgcttgctt caagaagttt ttgaaattct ccctggggta aaagtatgat
      781 ctgggaaaac tcctcatagt ttaggcccag aatttcatga atcttttcat tgacttccat
      841 aactcctttt gccacaggaa tatcatctat atgtaattca gcagtagaac tataagtaac
      901 aagggccttt cttttaccct gggcttcttg ggtaggcgcc ctgtaaattt caatcttctt
      961 gcctccaagt tcaaaaagga agttcacata gcaaagctcc tcctgagtat agtggctttt
     1021 aagggtatgg ctttctcttt ttttattgct tgcttctcca aaaagagcaa agctaatggc
     1081 atcaaaaata gtagtctttc ctgaacctgt tggaccactg attaaaaaaa gtcctccctg
     1141 atagaatttt gaaaaatcaa tttcaacttt ttgggcatag ggaccaaagg cattcatttc
     1201 aagtttaatc ggtttcacga ctactccttt caagcatttc tacaacaatc ttactctctt
     1261 cttttgttag ttcatttcct gttgactcta aataaaaact tttaaaaaga gataaatgat
     1321 ccaaccggcc aaggtcttct ccactttttg ttatcagatt gctttccatc tttgtatagc
     1381 ttagacctaa ggcctcagga taagattgtc ttaaacgatt catggcatca ggaatataag
     1441 acctatcctc cagctcaaaa taagacaaat ccggggagga gccttgcatt agatcattaa
     1501 aaaaccctgt aaataccctt atgtcctttg ataggctaag ggcgtgaaag tccacaaggt
     1561 ttttttgtgt atcaatcaca tagtagcctg gtgtttttcc tgcctcactt ttagaatact
     1621 tcatgggaga gcctgagtag tagatattgt ctgctacctg ctgggctgta tggatatggc
     1681 ctagggccac ataatcaaac tgattaaaaa gtgcggtgga cagattttct gttttcccca
     1741 aaaaaagtgg tctttccgaa tcactctcca gcaaggggtt ttttcctaaa acatagtggt
     1801 gggtcatcat tatattgaat ttatctgggt ctaagctaat agaagataga tgatgggcca
     1861 tggcttcttc caggtttgca aacctttttt catgaaaata gtttagctta aattgatcta
     1921 catagggcag gccgtagata tccacatcat ctatttcaat tttcgtaatt tctttaccca
     1981 caagacctat ataaagtccc atcccccaca gaagatctgc tgcataggca atgcgctcag
     2041 aactatcgtg gttgcctgga atcataacta cttttacgcc catttcacga cagagtttgc
     2101 ttaaaaaaag actcaaaagc tccaccgctt ctttaggggg aagactgcga tcataaatat
     2161 ctcccgctag taggacaagc tctatcttat gttttttgac cgcttcaata agaccctcca
     2221 gcatctcttt ttgtaggtca attaaagagc gctcattaag agactttcct aaatgtaaat
     2281 ctgcagtatg tagaatcttc attgtttttc ctttcctata catataaata aatcatagca
     2341 tatctagccc tacatagaag ctaataaaat aaataacatt cctatttcgt tttttttctt
     2401 gccaaaactc ataaaatgtg atagtttttg atagaaagac atccaaggag gttatgatgc
     2461 ttaaaggcag acatcttatt aatagcgaag acttcagcac ggaagaaatt caatctatta
     2521 tctctttggc tcaaacgatt attgagggtc ctcagcggtt ttcccacagt tgtgacggga
     2581 aactgcttgg gactcttttt tttgagcctt cgactcgcac aaggctaagc tttgaatctg
     2641 caatgcttcg tttgggagga cgggtaatcg gttttgccga tgaggccagc agttcttcta
     2701 caaagggaga aagtcttatc gatacggtaa acaccgtggc atcctatgca gatatactgg
     2761 ctatgcgcca ctctaaggag ggttcatcct atctagcctc tgcaaaatgc cccgtacccc
     2821 tgataaatgc aggagatggg ggccatcaac accctactca aacccttgca gacttactaa
     2881 caataactga aacaaaagga aaaacagaaa atctcgttgt tggtttttgt ggggacctaa
     2941 agtttggacg aaccgtccac tcccttgtta aggtaatgag tcgttttcca agcaacgatt
     3001 ttgtttttat ctcccctaaa gaactacata taccaaatca cttaaaatta gggctagagc
     3061 gctcaggagt aagttttatc gaggttgaac gtcttgagga agtcatagat aaacttgatg
     3121 tgctctatat gacccgggtg caaaaagaac gctttttcaa cgaggctgac tatatgcgcc
     3181 taaaggatag ttatatccta gataaaaaca aactaaaaag cgccaaggaa gacctagcca
     3241 tcctccatcc acttcctaga gtaaatgaaa ttcatcctga agttgatgat gacccaaggg
     3301 cactttattt taaacaggtt cgctatggca tgtatgttcg catggcgctg atcttaaaag
     3361 ttttgggggt agaagaatgt taaatataaa tagtattgcc aagggaatcg ttcttgatca
     3421 catcacccca ggcaagggat atgaaatctt taaactactc gagcttgata aagcaagcta
     3481 tacggttgct ttaattatga atgcacaaag caaaaagcac ggcagaaaag atattgttaa
     3541 aatagaaaat gttatcgata tggaccttcg ggttcttggt attttggatg aggatataac
     3601 cataaatatc at
//
LOCUS       DASRNA010000015         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_750948,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000015 DASRNA010000000
VERSION     DASRNA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750948"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..696
                     /gene="prxU"
                     /locus_tag="VFD08_00375"
     CDS             25..696
                     /gene="prxU"
                     /locus_tag="VFD08_00375"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083760882.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:223..225,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HZK09497.1"
                     /translation="MTAQPEEILQIEPKEKEKKHMIMVGKPAPNFTTSAFNKGEFTQE
                     SLEDYRGKWVVLCFYPGDFTFVUATEVAAVAAHNDQFEELGAQVLSVSVDSEFVHKVW
                     NDVELNKIAKKDIPFPMLSDSSGAIGELYGVYDREAGVNVRGRFLIDPDGVIQAMEVL
                     TPPVGRNVTETIRQLKAFQLVRETGGAEVTPSGWQPGGKTLKPGIELVSKVWEQWTIE
                     DAYKK"
     gene            complement(757..1887)
                     /locus_tag="VFD08_00380"
     CDS             complement(757..1887)
                     /locus_tag="VFD08_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZK09498.1"
                     /translation="MKKLGFGLMRLPLTDSEDSKTIDVDRFNTMVDLFMERGFTHFDT
                     AYMYHGEKSEEVFRESVVKRYPRESFTVTDKMPIYMVEKKEDLEKIFKEQLERCGVDY
                     FDYYWMHAMNDQRYSLSKEVDAFGFLEEKKKEGKIRHIGFSFHDSAEVLDKMLKNEPS
                     VEFVQLQINYLDWEDGRIQSRLCYETARKHNKKIFIMEPVKGGTLAQLPEQAEALLKK
                     KDPEASVASWALRYAASLEDVVMVLSGMSEEWQLEDNIEIFDDFKALEKEDYKLLDEV
                     VEIIKKSMKIACTSCRYCVDGCPVSIAIPEYFEIYNRSVDLSNPDDPAIKADFAKLAD
                     KHGKPWECIACGQCQDQCPQFLPIIEDLKKISDIFQKDYEKI"
     gene            complement(2002..3213)
                     /locus_tag="VFD08_00385"
     CDS             complement(2002..3213)
                     /locus_tag="VFD08_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZK09499.1"
                     /translation="MSKFAHIYKDAKGLQEELVTHRRYLHQHPELGLDLPLTSAYVKE
                     KLTEMGYKPLDCGQSGVLATVGGKNPGKVFLIRGDMDALPINEEVDWEFKSTNDNMHA
                     CGHDFHTSMLLGAAKLLKENEDKIDGTIKLMFQPAEETLEGAKAMIEAGILENPRVDG
                     AMMIHVISGQAVASGKIVLPIGGVGNAASDWFELYIKGKGGHGASPNNTVDPLNIAAH
                     THIALQGINSREIHSSHALALTVGVMEGGRVSNVIPDTAVLKGTIRTYDPEVREFVKT
                     RVKEIAEATAKVFRGETDARIIEGCPSIVNDRGLVDAANKYLLELLGEEAVMPITDLY
                     PSGRSSGSEDFGFLSEIVPTLSLSIGAGDSEEGYTYPIHHPKVRFDESVLARGSAVYA
                     YMALRWLEENK"
     gene            3457..4530
                     /gene="buk"
                     /locus_tag="VFD08_00390"
     CDS             3457..4530
                     /gene="buk"
                     /locus_tag="VFD08_00390"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HZK09500.1"
                     /translation="MTKRILVINPGSTSTKIAIYNNEDPLFIQSIEHSAQELASFEEI
                     SQQYLMRKEIILSVLKERGIELASIDVIVARGGILPPVKSGAYKINQAMVDQLRYHAV
                     VEHASSLGAIIAFSLAKDLALDAYIYDPVTVDEMEPIAKITGLPQMQRRALGHNLNMR
                     ASAIKYSKEIGADYQDLTLLVAHLGGGITLSLHHKGRMIDMITDDEGPFSPERAGGLP
                     AFQVIEMATQPGADYKSLMSKLRSQGGLKAHFGTTDAREIEEKIKNGDQEAALIYEAM
                     AHNVAKNIGKLAVVVEGKVDAIILTGGIAYSEMISKWIIQRVAFIAPVKVLAGENEME
                     ALALGVLRVVRGTEKAHLFTIEE"
BASE COUNT         1300 a          992 c          907 g         1384 t
ORIGIN      
        1 gctttatgtg agcgcactaa aaccatcact gctcagcctg aagaaatcct tcagattgaa
       61 ccaaaagaaa aggagaaaaa acatatgatt atggttggta aacctgcacc aaattttaca
      121 acatccgctt ttaataaagg cgaatttaca caagaaagtt tagaagatta cagaggaaaa
      181 tgggttgttc tttgtttcta cccaggtgac ttcacctttg tgtgagcgac agaagttgca
      241 gcagttgctg cgcacaatga tcagttcgaa gaactaggtg cacaagttct atctgtttct
      301 gtagatagcg aatttgtaca caaggtttgg aatgatgtag aacttaacaa gattgctaaa
      361 aaagatattc ctttccccat gctttctgat tcctcaggag ctattggaga actttatgga
      421 gtatatgacc gtgaagctgg cgtaaacgtc cgtggacgtt tcctaattga tccagatggc
      481 gttatccagg caatggaagt tcttacacct ccagttggcc gtaatgttac tgaaacaata
      541 cgtcaactca aagcttttca attggttcgt gaaaccggtg gagcagaagt cacaccttca
      601 ggatggcagc caggtggcaa aactcttaag cctggtattg agcttgtttc taaagtttgg
      661 gaacaatgga ccatcgaaga cgcctataaa aagtaaatct ctttcaatct tatacaaaaa
      721 aaccccgatt tatcggggtt ttttgctttc tcctaactag attttctcat agtctttttg
      781 gaaaatatca ctaatttttt taagatcttc gataataggt aaaaactgag ggcactggtc
      841 ttggcactgg ccacaggcta tacattccca tggcttgccg tgcttatctg ctagtttggc
      901 aaaatcagct tttatggctg gatcatcagg attggataga tcaacagagc gattatagat
      961 ctcaaaatac tctggaatgg caatacttac cgggcagcca tccacacaat acctacaaga
     1021 ggtacaagca attttcatgc tctttttaat aatctctact acctcatcaa gaagtttata
     1081 atcctctttt tcaagggctt taaaatcatc aaagatttca atattatcct ctagctgcca
     1141 ctcttcactc ataccactaa ggaccataac aacatcctct agtgatgcgg catatcgtag
     1201 agcccaggaa gcaacagagg cctcaggatc ttttttcttt agcagggctt ctgcttgctc
     1261 aggcagctgg gctagggttc ctccctttac tggttccatt ataaatatct ttttattgtg
     1321 ctttcttgcc gtttcatagc agagtcggga ctggattcta ccatcttccc agtctaaata
     1381 gttgatttga agttggacaa actctacaga aggttcgttt tttagcattt tatcaagaac
     1441 ttcagcggaa tcatggaagg aaaaacctat atggcggatc ttgccttctt tctttttttc
     1501 ttctaaaaaa ccaaaggcat ctacttcttt agacaagcta tatcgctggt cgttcatagc
     1561 gtgcatccag tagtaatcaa agtagtctac cccacagcgc tcaagttgct ccttaaaaat
     1621 tttttctaga tcttcttttt tttcaaccat atagatgggc attttatctg ttacagtaaa
     1681 gctctctctt gggtaacgct ttactacaga ttctctaaaa acttcttcgc ttttttcacc
     1741 atgatacata taggccgtat caaagtgggt aaaacctctt tccataaata aatcaaccat
     1801 ggtattaaag cggtccacat ctatggtttt gctatcttca gagtcggtta aaggcagtct
     1861 cattaagcca aatcctaatt ttttcattga ttgtcctttc tttcttgctt agatcagttc
     1921 ctttaaatta tagcataaga agaataaata ggctgaaaaa aaccccttgt gcttttttac
     1981 gcgccggggt tttatggttc tttatttgtt ttcttctagc cagcgtaggg ccatataggc
     2041 atagactgca ctcccacggg caaggactga ttcatcaaaa cggacctttg gatggtggat
     2101 cggataagtg tagccttctt ccgagtcacc tgctcctatt gacaaggaca gggtaggcac
     2161 gatttcactt aaaaatccaa aatcttctga gccagatgat cttcctgaag gatataaatc
     2221 tgtgataggc ataactgctt cttctcctaa gagctctaga agatatttat ttgccgcatc
     2281 aacaagtccc ctatcattga caatggaagg acagccctct atgattctag catcggtttc
     2341 tcctcgaaag accttggctg tagcctcagc tatttctttt actctagttt ttacaaactc
     2401 cctaacctct ggatcatagg tgcgaatggt acccttaagc acagctgtat cgggaatgac
     2461 attggaaact cgtccaccct ccatcactcc aacagtcagg gccagggcgt gggaagagtg
     2521 aatttcccta ctattaatcc cctgcagggc tatatgagta tgggcagcaa tgttaagggg
     2581 gtccactgta ttgtttggcg atgcaccatg gccacccttt cccttaatat aaagttcaaa
     2641 ccagtccgag gctgcattgc ccactccccc aataggaaga acaattttcc cactggctac
     2701 ggcttgtcct gatatcacat ggatcatcat tgcaccatct acccttgggt tttcaagaat
     2761 gcccgcttct atcatggcct tagcgccttc taaggtctct tctgctggct ggaacataag
     2821 tttaattgta ccatcaatct tatcttcatt ttccttaaga agtttggcag caccaaggag
     2881 cattgatgta tgaaagtcat gcccacaagc atgcatattg tcattggttg atttaaattc
     2941 ccagtctacc tcttcattga taggtaaagc gtccatatct ccacgaatta aaaacacctt
     3001 acctggattt ttccctccta ctgtggctag tactccagac tgcccacaat caaggggctt
     3061 ataacccatc tctgttaatt tttccttaac ataggctgat gttaagggta aatctagtcc
     3121 cagctcagga tgttggtgaa ggtagcggcg gtgagtaact agttcctctt gcagaccttt
     3181 tgcatcttta taaatatggg caaatttaga caaatcattt cctcctcttt ttataataca
     3241 tttacatatg taattttaag agtaacaaat tctcacccct gcgtcaattg ctccttttag
     3301 taatttttat tcttacttgt ccttctgctt attatttaat actaaaactg tttcttccta
     3361 gagacaaagg catagaccct tttgtccaat ctttgctata atgtagaaaa caaagaaaaa
     3421 tcactagtcg catatatcaa gttaagaggt ggccttatga caaaacgaat cttagtaatc
     3481 aatcctggct ctacatcaac aaaaatcgcc atatataata atgaagatcc tctttttatc
     3541 cagtctatcg agcattctgc ccaagaactt gcctcctttg aagaaatcag ccagcaatat
     3601 cttatgagaa aagaaattat tctatcggtt cttaaagagc gcggcatcga gcttgcttct
     3661 attgatgtaa ttgtggcccg agggggcatt cttcctcctg ttaaatcggg agcctataaa
     3721 atcaatcaag ctatggttga tcaattgcgc tatcatgccg tggtggaaca cgcctccagc
     3781 cttggcgcta ttattgcctt ttctcttgct aaggatcttg ccctggatgc ctatatttat
     3841 gatccggtta ctgttgatga gatggagcct atagcaaaaa ttacaggact tccccagatg
     3901 cagcgaagag ccctggggca caatttaaat atgcgggctt cggccattaa atactccaaa
     3961 gaaatagggg cagattacca ggaccttacc ctacttgttg cccatctagg cggtgggatt
     4021 acccttagcc tccaccataa gggtagaatg attgatatga ttaccgatga tgagggaccc
     4081 ttctcccctg aaagagctgg tgggcttccc gcttttcaag ttattgaaat ggcaacacag
     4141 ccaggagcag attataagag cttgatgtca aagctcagat cccagggagg gctaaaggct
     4201 cactttggaa caactgatgc cagagaaatc gaagaaaaaa ttaaaaatgg cgaccaagag
     4261 gccgccctta tctatgaagc catggcccat aatgtggcaa aaaacattgg taaactggct
     4321 gtggttgtgg aaggaaaagt tgacgctatt attttaacgg gaggaattgc ctactctgag
     4381 atgataagca agtggattat ccaaagggtg gcctttattg ctcccgttaa ggtgcttgca
     4441 ggagaaaatg aaatggaagc cctggctctg ggggttttaa gagtagtaag ggggactgaa
     4501 aaggcccacc ttttcacaat agaggagtag aaagagaaga ttatcgaaaa atacctattg
     4561 acatttaaat atcagtattt ttt
//
LOCUS       DASRNA010000016         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_153363,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000016 DASRNA010000000
VERSION     DASRNA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153363"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFD08_00395"
     CDS             complement(<1..199)
                     /locus_tag="VFD08_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK09501.1"
                     /translation="MFRPIRKKKQELSLEESIRVLKEGKLGVFALSDKEYPYALPMNY
                     IYYEEKIYVHSAKKGHKIDLIR"
     gene            complement(192..857)
                     /locus_tag="VFD08_00400"
     CDS             complement(192..857)
                     /locus_tag="VFD08_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZK09502.1"
                     /translation="MDFIQKLQKSPVFAGIEEEEIKELLTRPGCVIKKFPAGSLIHLK
                     GESCINVEYILKGEVTVQLLQETGNIMTISNFVQGEMLGSNLVFSSHPVYPMMISAKS
                     EVELLCLDAATILMLCRKSPVFLESYLKDISDRALVLSGKINTIALRSIREAVLDYLM
                     IERKRQKSDTINLPFTKKEWAERLGVQRPSLSRELSKMRQEGIIDYDRTSISLKDKAL
                     KNV"
     gene            946..1269
                     /locus_tag="VFD08_00405"
     CDS             946..1269
                     /locus_tag="VFD08_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK09503.1"
                     /translation="METKYVFNQSQEKLIEKIIDSDEVMINHMIFNESEGLPRHASNS
                     NVYMLVIRGTLSLKLGDKEEKKHPQGSLLSIAYNVMMDVSNKDREQVEIFVIKAPHPR
                     NYKEL"
     gene            1270..1752
                     /locus_tag="VFD08_00410"
     CDS             1270..1752
                     /locus_tag="VFD08_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK09504.1"
                     /translation="MDIFTLVLWVITLIWFFFSIFKDKKKTMHSVKMSTGMMKSIGGD
                     IVAILLMIGLILTLIPPESLEAFLVKNDTFFATVVFALIGSITLIPAFVAFPLAGSLT
                     GAGAGIMPIVAFITTLTMVGVVTFPLEKKEFGTKFVILRNSLSFVFAIVIAVAMGVLL
                     "
     gene            1749..2501
                     /locus_tag="VFD08_00415"
     CDS             1749..2501
                     /locus_tag="VFD08_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK09505.1"
                     /translation="MKKILDFAKNKKFLSFVLLVYASLLLFKTPLAIEAIKNSGYYFK
                     EMLMIMPVILLLTALLNAWVPKETIQKHLGSGSGMKGSVFSFILGSVSAGPIYAAFPI
                     AITLLKKGASVTNVVILLSSWAVVKIPMLANEAKFLGPKFMLMRWVFTTIAIFITGAI
                     TSRFVSREEILVLHEEEGKFKEDLSIHSEFCIGCGLCADISPEHFLMKNDLAVVVSQK
                     NLDKPALKKAVNSCPVQIIELSLTELRPIEAK"
     gene            complement(2571..2843)
                     /locus_tag="VFD08_00420"
     CDS             complement(2571..2843)
                     /locus_tag="VFD08_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09506.1"
                     /translation="MKNNKKMQAWSKYRQEGKTKYIHTKSLYIAAGVLVGSSLAGLLK
                     KDWMFFSLSNIMLIFLVTYFAARVGAAYFWKKSEEKYSQYIEDKKD"
BASE COUNT          844 a          595 c          522 g          984 t
ORIGIN      
        1 ttcttataag atctattttg tggccttttt tggcagagtg gacatagatc ttttcctcat
       61 aatagatata gttcatagga agagcgtagg gatactcctt atcactaagg gcaaagactc
      121 ccagtttccc ttcttttaag accctaatac tttcttctag gcttagctct tgttttttct
      181 ttcttattgg cctaaacatt ttttagcgcc ttatctttta agctaatgct tgtcctatca
      241 tagtcaataa ttccttcttg gcgcatctta cttagctccc tagaaagaga gggcctttgt
      301 actcccaagc gctcagccca ctcttttttt gtaaagggca ggttaatggt atcgcttttt
      361 tgcctctttc tttcaatcat tagatagtcc agcacagcct cgcgaatgct tcttagggca
      421 atggtgttta ttttcccact aagaacaagg gcccgatcag aaatatcttt taaataactt
      481 tctagaaaga caggactttt cctgcagagc ataagaatgg tagccgcatc taaacaaaga
      541 agttctacct cactttttgc gctaatcatc ataggataaa caggatgaga agaaaagacc
      601 agattgcttc ctagcatttc tccttgcacg aaatttgaaa tggtcataat gtttcccgtt
      661 tcctgcaaca gctgtaccgt cacttcccct tttagaatat actctacatt gatacagctt
      721 tctcccttta aatgaataag ggagccggcg gggaattttt taataacaca accaggccga
      781 gtaagaagtt cttttatttc ttcttcctca atgccagcaa aaacagggga tttttgtaat
      841 ttttgaataa aatccatctt tttcactcct agtgtaacct tggttacact ttattttctg
      901 tcttagttta tactagtaag tatatcatat gaaaagaggt caaaaatgga aacaaaatat
      961 gtatttaacc aatcacaaga aaaactaata gaaaaaatca ttgattctga tgaagttatg
     1021 atcaatcaca tgatctttaa tgagtccgag ggactaccta gacacgcttc taattccaat
     1081 gtctatatgc tggtcattcg aggaaccctt agtcttaaac taggagataa agaagaaaaa
     1141 aagcatcccc agggaagcct tctttctatt gcctataatg taatgatgga tgtttcaaat
     1201 aaggataggg agcaagtaga aatctttgtt attaaggctc cccatcctag aaactataaa
     1261 gagctttaga tggatatttt taccctagtc ctatgggtta tcactctaat atggtttttc
     1321 ttttctatct ttaaagataa gaagaaaacc atgcactcgg taaaaatgag tactggaatg
     1381 atgaaaagca ttgggggaga tattgtagca atcctgctta tgattggcct aattctcacc
     1441 ctgattccac cagaaagcct tgaagctttt ctggtaaaaa atgatacttt ctttgccacg
     1501 gtagtctttg cccttattgg gagtattacc cttatcccag cctttgtagc ctttccccta
     1561 gcgggatctc ttacaggtgc tggagcggga atcatgccca ttgtggcttt tatcaccacc
     1621 cttactatgg taggtgttgt aacctttccc ctagaaaaaa aagaatttgg cacaaagttt
     1681 gttatcctta gaaatagtct tagctttgtc tttgctattg taattgctgt tgccatggga
     1741 gtcttattat gaaaaaaata ctggattttg caaaaaacaa gaagttttta agctttgtcc
     1801 ttctagttta tgcaagctta cttcttttca aaacacccct agcaatagag gcaataaaaa
     1861 atagcggcta ctactttaaa gaaatgctta tgattatgcc ggtcatcctg ctactaacgg
     1921 ctcttttaaa tgcctgggta cccaaggaaa ccattcaaaa acatctaggt agcggatcgg
     1981 gaatgaaggg aagcgttttt tcttttattc ttggaagtgt ttctgctggt cctatttatg
     2041 ccgcctttcc catagctatt acccttctta aaaagggagc ttcggtcaca aatgtagtta
     2101 tccttcttag ctcctgggcc gtagtaaaga ttcccatgct ggccaatgaa gcaaagtttt
     2161 taggacctaa gtttatgctt atgcgctggg tttttactac aatagccatc tttattacag
     2221 gggctattac ctctagattt gtttcaagag aagaaatcct cgtcttacat gaagaggaag
     2281 gaaagttcaa agaagatctt tctattcatt ctgaattttg tattggctgt ggcctatgtg
     2341 ctgatatttc cccagaacat tttcttatga aaaatgatct tgctgtggtt gtaagtcaaa
     2401 aaaacctaga caagcctgca ctaaaaaagg cggtcaatag ctgtcctgta caaattatag
     2461 aactttctct tacagagctt cggcccatcg aggctaaata aaatttccaa agaaaaaacc
     2521 ggctactcaa ggccggtttt ttcttacttc atttctggaa ttatcccaga ctagtccttt
     2581 ttatcttcta tatactggct gtatttttcc tcgctttttt tccagaagta agccgcacct
     2641 actcttgctg caaaatatgt tactagaaaa atcagcataa tgtttgatag actaaaaaac
     2701 atccagtcct ttttaaggag ccctgcaaga gatgatccca ccaggacccc agctgctata
     2761 tatagactct ttgtgtgaat atactttgtt ttgccttctt gtctatactt actccaggct
     2821 tgcatttttt tattattttt cactttaact tccttttgtt cttccaaaag agcttttgtc
     2881 taggcccagt ataacataga aattctctgc attttttctt agccataaga aaaggactcg
     2941 agatg
//
LOCUS       DASRNA010000017        14629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1050440,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000017 DASRNA010000000
VERSION     DASRNA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14629
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050440"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..519)
                     /locus_tag="VFD08_00425"
     CDS             complement(226..519)
                     /locus_tag="VFD08_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZK09507.1"
                     /translation="MSRIRQRYDEDFKRNAVKLSYVTSKTMKELAADLGINASLIYNW
                     RRTYTEEGDKTRLAKQEDSLRKLQLENAELKMENEMLIPSGHRQKQRPTLPNI"
     gene            complement(1091..1681)
                     /locus_tag="VFD08_00430"
     CDS             complement(1091..1681)
                     /locus_tag="VFD08_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09508.1"
                     /translation="MENKNNWASAAPTLNLIFGMLSIMFAALFLGYLKPSAVYILGLI
                     QIGVWPGYLIGSLIMLRSGDIVGGNTFFYFATFFGLAPGLVTVGTYLADVFGWAYDPK
                     ILGIMWLSVGLTLTFTIPAFIKSELVTFLVIAAAGIAIDFIGLAYLFTGYAQTFNTIA
                     GVLLFFVGVGGIYQAVAGILAGAGIKLPLGKPIVKS"
     gene            complement(1731..2441)
                     /gene="fdhD"
                     /locus_tag="VFD08_00435"
     CDS             complement(1731..2441)
                     /gene="fdhD"
                     /locus_tag="VFD08_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZK09509.1"
                     /translation="MTIKNEKRAMYEEALVNLIVNNQALITFQCTPENLNELATGYLY
                     SRGLIRGLGDILEKNISNNKEKIFFTIKELAAEKEYVLPYELNKQRVKDFRIRGSKLQ
                     VSLRDIQKAFQEMCQSAVLYKETGGIHCAALADKGGLIICCEDISRHNAVDKVIGKGL
                     LMELDLTEYYLFTTGRMAADMVLKAVGSKVPMMVSPSIPSSMAVEIAKQEEITLVGRI
                     SKTPPLIYTYPSRIRMEV"
     gene            complement(2470..4377)
                     /locus_tag="VFD08_00440"
     CDS             complement(2470..4377)
                     /locus_tag="VFD08_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZK09510.1"
                     /translation="MSKELYGWAGTILRVDLSTGEITKESSEDKVKKFIGGMGLAAKL
                     MWDEIQPETQPFDPENKILFMTGPLTGTLAPTSGRMEVATKAPGVEPAACTRSNIGGD
                     FGPEVKYAGYDAIVVQGKAEKPVFILIEDDKVQILDAKEYWGKDTYETQKGIRKQFGN
                     LLKTVCIGQAGENLLNLSAVMSGSGHAAAKTGMGAVMGSKNLKAISVRGTGAVKIANK
                     EELIKLARETRRLLLHHPVRQWTCQGPMDVSIEFADAYRTKNNACFGCPVGCRSWIEF
                     PDPELEPGEMMCLANFYLWMGANHRDAWYGKVLTDKVGICQYTIYDMLRWLYECYEKG
                     IITEEQTDLPWSKYTTQEFLDVALDIMINRKGFGKYMAEGAYVTAQQLGDEAMEVYKA
                     YFPARNQSQHYSVRAHPAVLMQWATDSRDPLSYAHDWICLAYWQGNFWPKDQKGALTV
                     DQLKARAKEAWGSEAAGDVYTYEDKAQTTIVIQHMSRLKNSLVLCDWSIFPINTSLNN
                     PDYKGDPDMERKLYQAVTGEEVTVKEWIKIGERIFNMERAIGAREGRRKEDDTVDEYY
                     FRVPETEGAAWELKLDPPPVADREKFEKMREEYYTLRGADLETGIPTKEKLEELGLAD
                     VAEELIARKII"
     gene            complement(5122..6495)
                     /locus_tag="VFD08_00445"
     CDS             complement(5122..6495)
                     /locus_tag="VFD08_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK09511.1"
                     /translation="MSTIDYDKPTDFLLLGIAIPLIGKLLVAELYGIVDTFFVGRFLG
                     GASLGALGLVFPMQRFLFSLPILVGVGTSTLLSRSLGRGEKKEAGKIIASGALLLLFL
                     QLFFALTSFGKAREIMTLFGARGSSLSASSGYLGYASLGSIFFALNSFLSFVLLSLGN
                     TRVSIVSMLIGALSNAFLDPIFLGPLGFGIEGAAIASMISQFAGAFYAVIAFHLYLEK
                     KDIHFHYKPKINYYHLVFLGGLAAFIIEIEDSIVIYVLNRLLISTGGEEGVMILSIVT
                     KLTMFLFITLFGIASAMQPLAAYMYGAKKKIKLQELLRKTFFYSFTFTLAIWVVFMLR
                     AENLVGLFLQDGLGIKKTARVLRIVVAFFPISALYYLDIFYAQAKGEGKRALVSSLLR
                     QIFLLIPFSLLFVKGFNLGRTGVWISFPITDLSASLIAYISLKKQGIVQVFLPGKKIW
                     KKEMRKS"
     gene            complement(6594..8450)
                     /locus_tag="VFD08_00450"
     CDS             complement(6594..8450)
                     /locus_tag="VFD08_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZK09512.1"
                     /translation="MFFEELGPATSYRAGLYQIDDISLHWKKSLVLLCVLEGEITVRI
                     DDETYLRKKLDVDIINPGEMRSYKGLGPNRVLFIELCPIFFDDYVKGSSQIYYYVDQR
                     DTKKDQAKYSKLRRWMMMLAYEYFYPKEDSTSFSEDLLVDLMYHLLNQFHYLYYEQQE
                     LRDDLVELRRFHRIFTYLDVNFKEGASLQELAEQEYLSPSYLSYKIKDTLGLNFHDYL
                     GALRSEAGAKLLLSTDKSVSEIALDVGFSHPRYFVKRFEEVYHQIPQDFRELHSKKQE
                     DYKELTPDKKEFLLELAHKQRYHFNGNSKTISVDLNKETLASFERAQCIFLGEAVYYL
                     EKETQDLLKRVQKEIGFKQGILAKLFSQDMDIYQDHSRRFHNWTRVESVLELLVQEGL
                     SPLIYCKGVENDVLEDFIQTFSPIYPGLEDWLIKKAEAELIFSSDQDQFSQAIEVIKN
                     PRDIPLLVDQMSREIPLENDTFFGGEGLFTANFLAKPSYYARGFLAMMGDELLEEGPG
                     YLLTRSREGYQLFFYQEESPKKIAPSLTLYGMKEDMSLSRFTLSARYGSIYDAWKNLG
                     SPKRLEKEYWDFMEAYIHPLLEFEYVERENLFSVRRQSKEAEITLYLFKEIQ"
     gene            8613..9527
                     /locus_tag="VFD08_00455"
     CDS             8613..9527
                     /locus_tag="VFD08_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZK09513.1"
                     /translation="MEAKDKLLALLMVIVWGANFTIIKLGLGGIPSMLLVAVRYLLVF
                     FPAIFFVKKPASSWKYIIAYGFTVGVGQFSCLFYALEIGMPAGLASIIVQLQGFIAPF
                     LSALFLDEKLSAKQLVGFFVAALGLVLIGLASSSQGVTSIPFSAFLLTIGAALFWSVS
                     NIISKSASIEAKKEGKQLDMFALVVWSSLIPPLPMFGLSLLINTPSLIISSLSTLSLM
                     SVFSVLYLAYASTLFAYKVWNDLISKYPMAKVSPISLLIPIFALLVSQLILSEELSKM
                     QWLGVVVILAGLLISNLTPKKRPSPPKP"
     gene            complement(9622..10278)
                     /locus_tag="VFD08_00460"
     CDS             complement(9622..10278)
                     /locus_tag="VFD08_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HZK09514.1"
                     /translation="MDKKQTQDFIAKRTAKELSNGDLVNLGIGLPTRVSNFVADDINV
                     IFQSENGFVGLGPEPKEDEVDPHIVNAGGMPSSILPGGMFFDSATSFGIIRGGHLDFT
                     VLGALQVDEKGNIANYMIPGKMVPGMGGAMDLLSGAKKTIVAMEHLARGRHKILKECN
                     LPLTAVAAVDLIITEMCVLEVTDKGLLMTEINPDYTVEEVQEATEATLIIADNLKEMK
                     "
     gene            complement(10284..10937)
                     /locus_tag="VFD08_00465"
     CDS             complement(10284..10937)
                     /locus_tag="VFD08_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZK09515.1"
                     /translation="MNKLISIEEAVAKIKDGDTIMVGGFLANGTPEKLIDALVEKGVK
                     DLTLICNDTGFTDKGVGKMVVTKQFKKIIASHIGTNRETGNQMNSGETEVVLVPQGTL
                     AEQIRAGGSGLGGVLTKTGLGTKVEEGKEKVMIDGEEYLLEKPLTADVALIYGTKVDK
                     FGNIYYKGSTQNFNVLMAAAAHTVIVGAEEVVEVGELDPNFVRTPGVFVNFIVEEGL"
     gene            complement(11088..12398)
                     /locus_tag="VFD08_00470"
     CDS             complement(11088..12398)
                     /locus_tag="VFD08_00470"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZK09516.1"
                     /translation="MKANDLLLMSKMHLDEAEVYLSSSTTISLSLFEGELDNYEMAES
                     GGLGFRGIKGGRIGYSYSESLDVDVFPELINNVLASLEVMEEKEELFDGNAIYPQVKT
                     RELIPQSPDKVLKELQRLESLILKTPGITKVPGIYYQEITSKTTIKNTLGLSKTNEAG
                     VGIIYGMAAAQKDGQMYTGKAMRVFYDFSELDLTDLAEEITSDVLSSLGAQSIASGDY
                     RVFIDKKAFDSLLASFSSTFNAESVQKGMSLLKGKIGEKIASEKVNLLMAPLDERAPL
                     PSSFDGEGYPTKNFHLIKKGVLKSFYHNQATAKKEGVSSTGNAARSYKGKVGLSPNFL
                     ILRAGEKSEKEIIKSMQSGVYIKEMTGFHSGLNAISGDFSLPAAGYLIKEGEKLHPVN
                     QILLSGNFFTLIKDILELSSETSSESLSSTTPGVLVDKLSVGGK"
     gene            complement(12388..13773)
                     /locus_tag="VFD08_00475"
     CDS             complement(12388..13773)
                     /locus_tag="VFD08_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZK09517.1"
                     /translation="MTQEKARRVLEAALSGGGDFAEIFLENKKNNSLQLIGGEIRSAI
                     SGRDYGLGLRVIKGLKSVYSFTNNTSEENLLALALKSARALEGDKINQVLDFEQKKPK
                     NLNWIKHDPGNYSLKEKEEALRQAYEAAMAYDELISQASISYMDWDQEVLIINSQGLW
                     VEDKRVRSRFAVSAVADYKGEKQTGFRGPGASMGMELLEKYPPKETAEEAARIAVTMA
                     RAGYAPSGEMPVIIDKGFGGVLFHEACGHSLEASSVAKKNSVFTDKLGEQIASKKVTA
                     IDDGSLPGEWGTTTVDDEGSPTRKNILIEEGILKGYLVDRLNGKRMKMQSTGSARRES
                     YRYEPTSRMNNTFIAPGEDKFEEMIATTPRGLYAKSLGGGSVNPITGEFNFAVMEGYL
                     IEKGKITKPVRGASLMGKGHEILMKIDAVSSDFALAEGMCGAASGSIPASLGQPQVRI
                     SSLKIGGRNEG"
     gene            complement(13770..>14629)
                     /locus_tag="VFD08_00480"
     CDS             complement(13770..>14629)
                     /locus_tag="VFD08_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK09518.1"
                     /translation="KTPFIVNSVGLILNMVLDPIFIFSLDMGIQGAALATALANFSVS
                     LIYFLIRKKEGLLANINFLKDFCFDKVKRILSWGYPAAVYNIFFVSVSTVISRQVTAF
                     SSGAFAAFEVGVQIEAIGWMMFGGLATTLGVFVAQNYGAENYERMLEGYHKGTKIASV
                     IGFVALLALYFFSGQLMGLFIKDDPYALSAGMNYLRIVALCQIFSSWEASSQGGFNGL
                     GKTKLPSVVGVLTNLSRIPLSYFLKGYFGLNGIWIALSISAGLRGIFLRVLFHFEKKR
                     YLVLVEEEQ"
BASE COUNT         4217 a         3272 c         2421 g         4719 t
ORIGIN      
        1 gtgtgtccct gtcttctcag tgatccacag acacgatcag gaccgtaggt tttatggcta
       61 tcattaaaga tctttacaat ggctttttca tacttttttt gcttatcatc ccgatcctct
      121 ttcgttttca accagtcata gtaagcactg gtcgaaaact ttagcatagc ggcccacttt
      181 gtaagaggat agttattgtt cttttgaaca aagcggtatt ttacttcaga tgtttggcaa
      241 agtaggccgc tgcttttgcc tgtgccctga gggtattagc atttcgtttt ccattttaag
      301 ttctgcattt tcaagctgaa gttttcgaag agagtcttct tgcttagcaa gcctggtttt
      361 atccccttct tcggtatagg ttcttctcca gttataaata agactggcat taatccctaa
      421 atcagcggca agctctttca ttgttttcga tgtgacgtaa cttaatttta ctgcatttct
      481 tttaaaatct tcatcgtatc tctgccgaat tctagacata ataaatactc cttccatata
      541 gtactatctt atcattatgt ccggaattat aacatggacc ccaaccttgt aagttgttaa
      601 gtgacaataa caaggaaaga actcattcac aagccatttt accacacatc taatataaat
      661 tctctataat ggtaaatcaa atcagtggag taattcaact aaaataaaaa gttaaatatg
      721 tgtttaagtt ttgatatgac taataagttc cacttctcac tttgatacaa ttttattgtg
      781 ctaactagca aaaaaacacc cgaactatga aaagtcagcc ctaaagtgtc ttttttatcc
      841 tttttctttt tatttttttt ctttgacaat atctatgtga ctctataata ccttgctgta
      901 agattaatgc aaacaaaatt agataaggcc ccttgattta tgtctaaaac actatttata
      961 agatagactg taaaaaaata aagcaaggca ggaatcgttt tgccttgctt tatttatgcc
     1021 agctattttc taatgttagt ttaatcccat cattctcaat tgcttattta gattatatat
     1081 gtaacctgcc ctagctttta acaatgggct tgccaagggg gagttttatt ccagcacctg
     1141 ctaatatacc tgcaactgct tgatatatcc ctccaactcc aacaaaaaac agtagtactc
     1201 cagcaatggt gttaaaagtt tgagcataac cagtaaatag atatgctaaa ccaataaagt
     1261 ctatcgctat ccctgcagca gcaataacaa gaaaagtaac cagttctgat ttaataaaag
     1321 caggaatcgt aaaggtaaga gtcaatccta cggataacca cataatccct aaaattttag
     1381 gatcataggc ccagccaaaa acatctgcta gatatgtacc aactgtaacc aaaccaggag
     1441 ctaagccaaa aaatgtagca aagtaaaaaa aagtattacc ccctactatg tccccactgc
     1501 gtaacataat caatgatcca attaaatatc ctggccatac cccaatttga atcagaccaa
     1561 gaatataaac agctgatggt ttcaaataac ccaaaaacaa agctgcaaac attatagaca
     1621 gcatcccaaa aattaagttt agtgttgggg ctgctgaagc ccaattgttt ttattttcca
     1681 tattttacgt cctttcttac ttctttattt aagtttctaa tatcctctcc ttacacttcc
     1741 atccttatcc tagatgggta ggtgtagata agaggggggg tttttgatat tcttccaact
     1801 agtgttattt cttcttgttt tgcaatttct acagccatac ttgatgggat actaggagaa
     1861 accatcatag gaactttgct accaacagct tttaaaacca tatcagctgc catgcgacct
     1921 gttgtaaaaa ggtaatactc agttaggtct aattccataa ggagtccttt tccaataact
     1981 ttatcaactg cattatgtcg acttatatct tcacagcaga tgattaagcc ccccttatca
     2041 gctaaagctg cacagtgtat tccccccgtt tctttgtaaa gaacagcaga ttgacacatc
     2101 tcctggaagg ccttttgaat atctcttaga ctaacctgca gttttgaacc cctaattcga
     2161 aaatctttca ccctttgctt attcagctca tagggtaaaa catattcttt ttctgctgct
     2221 agctctttta ttgtgaaaaa tatcttctcc ttattattag agatgttttt ttccagaata
     2281 tctcctaaac ctcgaattag cccccggcta tagaggtagc cggtggctaa ttcatttagg
     2341 ttctcaggag tgcattgaaa ggtgattaat gcttgattgt taacgattag attcacaaga
     2401 gcttcttcat acatagcccg tttttcgttt tttattgtca tattccttta tcctctaaaa
     2461 aagtatagat tagataatct ttctagctat taattcttct gcaacatctg ctaaacccaa
     2521 ttcttctagt ttttctttag tgggaatgcc cgtttctaga tctgcccctc ttagagtgta
     2581 gtattcttct ctcatttttt cgaatttttc tctatcagca acaggtggtg gatctagctt
     2641 taattcccaa gctgcacctt cagtttctgg tactctaaag tagtactcat ctactgtatc
     2701 atcttctttt ctgcgtcctt cacgagctcc aatcgcacgc tccatgttaa agattctttc
     2761 gccaattttt atccactctt ttaccgttac ttcttcccca gttacagcct ggtatagttt
     2821 tctttccata tctggatcac ccttatagtc tggattattc aaactagtat ttatcggaaa
     2881 tatagaccag tcacaaagaa ctaaactgtt tttaagtcgc gacatatgct ggataacgat
     2941 agttgtttgt gccttgtcct cataggtata aacatctcct gctgcttcac taccccaagc
     3001 ttccttggcc ctagctttaa gctggtctac tgttagggct cctttttgat cctttggcca
     3061 gaaatttcct tgccagtaag ctagacatat ccagtcatgg gcataggata aggggtctct
     3121 agagtctgtt gcccattgca ttaaaacggc agggtgtgct cttacagaat agtgttgact
     3181 ttggtttcta gcggggaagt aagccttgta cacttccatt gcttcatcac caagctgttg
     3241 ggctgttaca taagcacctt cagccatgta ttttccaaaa ccttttctat taatcataat
     3301 atctaaagcc acatctaaaa attcctgtgt tgtgtattta gaccatggaa gatcggtttg
     3361 ttcttctgta ataatgccct tttcatagca ttcatataac cagcgcaaca tgtcataaat
     3421 agtatattgg caaattccta ctttatcggt tagaactttc ccataccatg catctctatg
     3481 attggctccc atccatagat agaaattagc caaacacatc atttctccgg gctctaattc
     3541 tgggtctgga aattcaatcc atgacctgca acccacagga cagccaaaac atgcattgtt
     3601 ttttgttcta tatgcatcag caaattcaat gctaacatcc attggaccct gacatgtcca
     3661 ttgtcttact ggatgatgca atagcaatct tcgggtttct ctagccagct taataagttc
     3721 ttctttattt gctattttaa ctgcgccggt tcctctaacg ctaatcgctt taaggttttt
     3781 agagcccata acagcgccca taccggtctt agcggctgcg tggcctgagc cactcataac
     3841 tgctgagaga tttagtaggt tttctcctgc ttgaccaata caaactgttt ttaataaatt
     3901 tccgaattgt tttctaatgc ctttttgcgt ttcatatgtg tctttacccc aatactcttt
     3961 ggcatccaaa atttgcactt tatcatcttc aatcaagata aaaactggtt tttcagcctt
     4021 cccttgaaca acgatagcgt catatcctgc gtatttaact tccggtccaa aatctccccc
     4081 aatgtttgaa cgagtacagg cggcaggctc aacaccagga gcttttgtgg caacttccat
     4141 cctgcctgat gtaggggcta gtgtcccggt caagggacct gtcataaaca atattttgtt
     4201 ttctgggtca aagggctgag tttctggttg gatttcgtcc cacattaatt tagctgctaa
     4261 gcccatacca ccaataaatt ttttcacctt atcttcagag gattcttttg taatttctcc
     4321 tgtacttaga tcaactctaa gaattgtccc tgcccatcca taaagttctt tactcatcgc
     4381 tattctcctt atatctaaat actccagctt ggcaccatcg catgcaactt ttacatccaa
     4441 tgcaattttc aattgtaatg tctgcttttg ctgtaaatag atcaaagttt acttttaccc
     4501 ttgataactc tgtactacat actccctcat tttccaatga gcatatcatc tcacaaagtc
     4561 tacagcctag acacctttct ggatacatag caactacttt acccataata ctaaccctcc
     4621 tcttttttat taacgtctac tgtcgcttct tatagctttt tgtactaaaa aaaactctgc
     4681 aaataatgcg gagcctttcc aagagtggga ggcagttata aactacccta acggtctact
     4741 ttaaaaaaaa tcgactcggt tcctacctac atttttatac tatctagcta ctttgtcaaa
     4801 ctttataatc taaatggtaa atggggaggc caagtttctt ttcaaaataa acacaacact
     4861 tgcttttgct agtatccaac ttagcttagg aaacaaaccc ctcttattta ccttgacagc
     4921 tagccacaag acggaagtat ttataaaacc ttggcgctag tataaaagta aaaataagaa
     4981 agtgtaaaag gccgtttgac acaatagtta tgcaaaccgc aacaaaaaaa gaataagcaa
     5041 agttcaatat actcacatct agagtctaat ttataaaaac taaatagcaa tctcttatat
     5101 tatatagttt ttcatcagaa tttaactctt tctcatctcc tttttccata tttttttccc
     5161 tggtaagaag acttgaacaa tcccttgttt ttttaggctt atataagcta taagacttgc
     5221 tgataaatca gtaataggaa agcttatcca gactcctgtc cttccaagat taaagccttt
     5281 tacaaaaaga agagaaaagg gtatcagaag gaagatttgc cgaagtaaag atgaaacaag
     5341 tgctcttttt ccctcccctt tggcttgagc ataaaaaata tctaaataat aaagggcgct
     5401 tataggaaaa aatgctacaa ctatacgtaa gacccttgct gtttttttaa tccccaggcc
     5461 atcttggagg aaaagaccta ctagattttc agctctaagc ataaaaacaa cccaaatagc
     5521 cagggtaaag gtaaagctgt agaaaaaagt ttttcttagg agctcttgta attttatctt
     5581 ctttttcgcc ccatacatat aggctgccag gggctgcata gctgaagcaa taccaaaaag
     5641 cgtgataaaa agaaacatgg taagtttggt cacaatgctt aaaatcataa ctccttcttc
     5701 tcccccagtg gaaataagca agcgattcaa tacataaata acaatagaat cctctatctc
     5761 tataataaaa gcagcaagac ctcctaaaaa cacaagatgg tagtaattta tctttggttt
     5821 atagtgaaag tggatatctt ttttttctag atataagtga aaagcaatta cagcataaaa
     5881 ggcccctgca aattgactaa tcatactagc tatggctgct ccctcaatac caaagcccaa
     5941 aggaccaaga aagattggat ctaaaaaggc gttggaaagg gctccaataa gcatagaaac
     6001 aatagaaact cttgtattac ctaaactaag aagaacaaag gataaaaaac tatttagggc
     6061 aaaaaatatc gaacccaggc tggcatagcc taaatagcca ctactggcgc ttaagctact
     6121 gcctcgagca ccaaaaagtg tcattatttc cctggctttt ccaaaactag taagggcaaa
     6181 aaaaagctgt aaaaacaaaa gaagtagagc tccacttgct ataatttttc ccgcttcttt
     6241 tttttctccc ctacccaggc ttcttgaaag caaggtagaa gtacccaccc caacaagaat
     6301 aggaagggaa aataaaaacc tctgcatggg aaaaacaaga cccagcgccc ctagggaggc
     6361 cccaccaaga aaacgtccta caaaaaaagt gtctactata ccataaagct ctgcaacaag
     6421 aagttttcca attaaaggta tggctatacc taaaagcaaa aaatctgtag gcttatcata
     6481 atcaattgta ctcataaaaa tctccttctt tcatcttact gcaataaagg aaggaaatca
     6541 tggcttctta ttggctcttt ggtggttttt ttagccttct tccatattta cttctactgg
     6601 atttccttaa ataagtacaa agtaatctcc gcctctttgc tttgacgccg cacagaaaaa
     6661 aggttttctc tttctacata ttcaaattct aaaagggggt gaatgtaggc ctccataaag
     6721 tcccagtatt ctttttctaa gcgcttgggg gaacctaggt ttttccaagc atcataaata
     6781 gaaccataac gggcactaag ggtaaaacga cttagtgaca tatcttcttt cattccataa
     6841 agagtaagag aaggtgcaat ctttttaggc gactcttcct gataaaagaa cagctggtag
     6901 ccctccctac ttcttgtaag aagataacct ggaccctctt ctaggagctc atctcccatc
     6961 attgctaaaa accctctggc ataatagctg ggttttgcta aaaaattagc cgtaaaaagt
     7021 ccttccccac caaaaaaggt atcgttttca agggggattt ctcggctcat ctgatctact
     7081 agaagaggaa tatcccttgg gtttttgatt acttcaatag cctggctaaa ctggtcctga
     7141 tcagatgaaa agatcagttc tgcttctgct ttctttatta gccagtcctc tagcccagga
     7201 taaatcgggc taaaggtctg aataaaatct tccaagacat cattttctac ccctttgcaa
     7261 tagataaggg ggcttaggcc ctcttgaaca agaagttcta agacactttc taccctggtc
     7321 cagttatgga atcttctact atgatcttgg tatatatcca tatcttggct aaagagtttt
     7381 gccaaaatac cctgcttaaa gccaatttct ttttgcaccc gttttagcaa atcctgggtt
     7441 tctttttcaa gataatagac agcttctccc aaaaaaatac actgcgccct ttcaaaagaa
     7501 gccagggtct ctttatttaa atccaccgat atggtttttg aatttccatt aaaatgatag
     7561 cgctgcttat gagctagttc caaaagaaat tctttcttat caggagttag ttccttatag
     7621 tcctcttgtt ttttgctgtg taactctcta aaatcctggg ggatttgatg atagacttct
     7681 tcaaaccgct tgacaaaata tcggggatgg gaaaacccta catcaagggc tatctcacta
     7741 acacttttat ccgtagatag gaggagcttg gctccagcct cactacgcaa agcaccaaga
     7801 taatcatgaa aattaagccc taaggtatct tttatcttat aagaaagata gctgggactt
     7861 aaatattctt gctcagctag ttcttgtaac gaggcgccct ctttaaaatt aacatctaaa
     7921 taggtaaata tacgatgaaa gcgcctaagc tctactagat catctcttag ttcttgctgc
     7981 tcataataaa gataatgaaa ttgattgaga agatgataca tcaaatcaac cagcaggtcc
     8041 tcactaaagc ttgttgaatc ctctttagga taaaaatact cataggccag catcatcatc
     8101 cagcgccgca acttactata cttggcttga tctttttttg tatccctctg gtcaacatag
     8161 tagtagatct ggctactgcc cttaacatag tcatcaaaaa aaatgggaca tagctcaata
     8221 aatagcaccc tgttggggcc aagcccctta taggagcgca tttctcccgg attgatgata
     8281 tctacatcca acttttttct aagataagtc tcatcatcaa tcctaacggt aatctcccct
     8341 tctaaaacac aaagaagtac caggcttttt ttccaatgaa ggctgatatc atctatttgg
     8401 tatagccctg ccctatagct agtagcagga cccaattctt caaaaaacat agcaaccacc
     8461 tttactaaat ttattataac agactaaagc gtcctatcta caaagccttt ttttaccctg
     8521 aaattacctg cctatctttt ttctaacaat aatattatta tgcccttttt tatgctattc
     8581 tatctataga aagcaagctt tggaggacga aaatggaagc taaagataaa ctactggcgc
     8641 ttcttatggt cattgtatgg ggagcaaatt ttactataat aaaactagga ctgggtggta
     8701 tcccctctat gctacttgta gcagtgagat acttgcttgt cttttttcct gccatatttt
     8761 ttgttaaaaa gcctgctagt agttggaaat acatcatcgc ttatggtttt actgtaggtg
     8821 tgggccagtt ttcctgcctt ttttatgcct tggaaattgg aatgcctgca ggacttgctt
     8881 ctattattgt ccagcttcaa ggatttatcg ctccttttct aagcgctctt tttttagatg
     8941 aaaaattaag tgcaaagcaa cttgtcggtt tttttgtcgc cgcactgggc cttgtcctta
     9001 ttggtctagc ttcctcttcc caaggagtta cctccattcc ttttagtgct tttctactaa
     9061 caattggggc cgctttattt tggtctgttt cgaatattat ctctaagtcc gcttccattg
     9121 aggccaaaaa agaaggaaag cagctcgaca tgtttgccct tgtcgtatgg tcaagcctta
     9181 ttcctcctct tcctatgttt ggactttccc tgcttattaa tacaccttct cttattatta
     9241 gttctttgag cactcttagt cttatgtctg tattttctgt tctttatcta gcctatgcct
     9301 ctacactctt tgcttataaa gtctggaatg atcttatttc aaaataccct atggccaagg
     9361 tgtccccgat ttcactgcta attcccattt ttgcccttct ggtttctcaa cttatactct
     9421 cagaggagct ctcaaaaatg cagtggcttg gtgtagtagt aatccttgct ggtcttctaa
     9481 tttcaaacct tacccctaaa aaaaggccct cccctcctaa gccttaatag gcatatatag
     9541 gctttgtttc acaagaaaaa agccccagct ccatctgaaa atggagttga ggcttttatt
     9601 attcttgcta tttctagctt cttatttcat ttcttttaga ttatcagcaa tgattaaagt
     9661 agcttctgta gcttcttgta cttcttctac agtataatca ggattaatct ctgtcattaa
     9721 aaggcccttg tctgttactt caagtacaca catctctgtg ataataaggt ctactgcagc
     9781 aacagctgtt aggggtagat tacactcctt aagaatctta tgtctacctc tagctaggtg
     9841 ttccatggct acgattgttt ttttcgcgcc acttaaaagg tccatagctc cgcccattcc
     9901 aggaaccatt tttccaggaa tcatatagtt agcaatgttt cccttttcat ctacttgtag
     9961 agcacctaaa actgtaaagt caagatgtcc accgcggata attccaaagg atgttgcaga
    10021 atcaaaaaac attccgccag gaagaatact agaaggcatt ccacctgcat taacgatatg
    10081 aggatctact tcatcttctt ttggttcagg tccaagtcca acaaagccat tctcactttg
    10141 gaaaataaca ttgatatcat cagcaacaaa gttagatact cttgttggaa gaccaattcc
    10201 taggttaact aggtcaccgt tacttagttc tttagctgtt ctttttgcaa taaaatcttg
    10261 tgtttgcttt ttatccatca tttttacagc ccctcctcta caataaagtt tacaaatact
    10321 cctggtgtgc gaacaaagtt aggatcaagt tctcctactt caactacttc ttctgcacca
    10381 acaataacag tatgagcagc cgctgccata agaacattaa agttctgggt tgaacccttg
    10441 taatagatgt ttccgaattt atctactttt gttccataaa tcagtgcaac atcagcggta
    10501 agaggttttt caagaagata ttcttctccg tcaatcatga ctttttcttt accttcttct
    10561 acctttgtgc caagaccagt tttggtaagt acaccaccaa gaccagaacc gccagcgcgg
    10621 atttgctcag ctaatgttcc ttgaggaact aaaactacct cagtctcacc actgttcatt
    10681 tgatttcctg tttccctatt cgttccaata tgggaagcaa taatcttttt aaattgtttt
    10741 gtaacaacca ttttgccaac acccttgtcg gtaaaacctg tgtcattaca aatcagtgta
    10801 aggtctttga cgcctttttc tacaagagca tcaatgagtt tttcaggagt tccattggct
    10861 aaaaagcctc ctaccattat ggtatctccg tctttaattt tagcaacagc ttcttcgatt
    10921 gaaattaatt tgttcatgca tccacctttc cttttcacat aataagcgtg catctactac
    10981 ttctcattat aacacctatt tactgctaaa tacaaggcca tagcagaatg ttgacatcta
    11041 tttatcaata ttttgctatt cctttgtttg aataatgttc agttttatta ctttccccca
    11101 acacttagtt tgtcaaccag cactccaggg gttgtgctcg aaagactttc tgaggaagtt
    11161 tcactagaaa gctccaaaat atcttttata agggtgaaaa agttaccaga aagcagtatt
    11221 tgattcacag ggtgaagctt ttccccctcc tttataagat aaccagcagc tgggagagaa
    11281 aaatctcccg atatggcatt gagtcctgaa tgaaatccgg tcatctcttt aatgtatacc
    11341 ccactttgca tacttttaat aatttctttt tcactcttct ctcctgctcg aagaataagg
    11401 aagtttggac taaggccaac tttgccctta taacttcttg cagcattacc cgtagagctt
    11461 actccctctt tttttgccgt ggcttggttg tgataaaaac ttttaagaac gcctttttta
    11521 attaggtgaa agtttttagt tggatagccc tcaccatcaa agctacttgg aagaggagct
    11581 ctttcatcca agggcgccat aagcaggttc actttttctg aagcgatttt ttcaccgatt
    11641 ttacccttta acaaactcat tcccttttgt acgctctcag cattaaatgt cgaagaaaag
    11701 ctagcaagca ggctgtcaaa ggccttttta tcaataaata ccctatagtc tcctgaagct
    11761 atactttgtg ctcctaggct agagagaaca tcactagtta tttcttctgc aaggtctgta
    11821 aggtccagct cagagaagtc ataaaaaact cgcatagctt ttcctgtata catctgccca
    11881 tctttttgcg cagctgccat accatatatg atgcccactc ctgcttcatt tgttttagaa
    11941 agacctaggg tgtttttaat agtggttttt gatgtgattt cctgatagta aataccagga
    12001 accttggtaa tccccggagt cttgaggata aggctctcta gtctttggag ctcttttaat
    12061 accttatcag gggattgtgg aataagctct cttgttttca cctgaggata aattgcattt
    12121 ccatcaaaga gctcttcttt ttcttccatc acctctaaag aggcaagcac attatttata
    12181 agctctggaa atacatctac atctaggctt tcactatagc tatagcctat acgtccccct
    12241 ttaattcccc gaaatccaag cccaccactt tctgccattt catagttatc aagctcgcct
    12301 tcaaagaggc taaggcttat tgttgtagag gaactcaagt aaacttcagc ttcatcaaga
    12361 tgcatttttg acatcagtag aagatcatta gccttcattt ctacctccta ttttcagtga
    12421 tgaaatcctt acttgtggct ggcctaaaga agcgggaata gaaccgcttg cggccccaca
    12481 cattccttca gctagggcaa aatcactact tacagcatca attttcatca gaatttcgtg
    12541 cccttttccc ataagagaag ctcctcttac tggtttggta atttttcctt tttcaatcag
    12601 atagccctcc attaccgcaa agttaaattc tcccgtaata gggttgacac tacctcctcc
    12661 tagacttttt gcataaagac cccttggtgt agtggctatc atctcttcaa atttatcttc
    12721 tcccggcgca ataaaagtat tgttcatgcg agaagtgggt tcatagcgat aagactctcg
    12781 cctggcagag cctgtgcttt gcatcttcat tcttttgcca tttaagcggt ccactaaata
    12841 acctttgagt ataccctctt caattaggat attttttctt gtaggactac cctcatcatc
    12901 aactgttgtt gtaccccatt ctcctggcag gcttccatca tcaatagctg tgactttttt
    12961 actggcaatt tgttctccca gtttatcggt aaagacggag tttttcttgg ctactgagga
    13021 agcttctagg ctgtgaccac aggcttcatg gaacaaaact ccaccaaagc ccttatcaat
    13081 aattacaggc atttcgcccg agggagcata tcctgccctt gccatggtta cagcaatgcg
    13141 agcagcttct tctgcggttt ctttcggagg gtatttctcc aggagttcca tacccataga
    13201 ggcacctggc cccctaaatc ctgtttgttt ttctccctta taatccgcta cagcactaac
    13261 agcaaagcga cttcgcactc ttttatcctc aacccataga ccctggctat ttataataag
    13321 cacctcctga tcccagtcca tatagctaat actggcctgg ctaattagtt catcataggc
    13381 catggctgct tcataagcct gcctaagggc ttcttccttc tccttcaggg aatagttgcc
    13441 tggatcatgt ttgatccagt ttagattttt tggctttttt tgctcaaaat ctaagacctg
    13501 gttgatttta tccccctcaa gggccctggc actttttagc gccagggcaa gcaggttttc
    13561 ttctgaggta ttatttgtaa aagaatatac actctttaga cctttaatca ctctcagccc
    13621 aagaccatag tctcttccgc taatggcact gcgtatttct ccgccaataa gttgtaggct
    13681 attatttttt ttgttctcaa gaaatatttc tgcgaaatct cccccaccac taagggcagc
    13741 ttcaaggact cgtcttgcct tttcctgtgt cattgttcct cctctactag tacaagatat
    13801 cttttctttt caaagtgaaa taggacccga agaaaaatac cgcggagtcc tgccgaaata
    13861 ctaagtgcta tccaaatgcc attaagacca aaatacccct taagaaaata agaaagggga
    13921 atcctagaaa ggttggtaag aactcccacc accgagggaa gttttgtctt tccaagccca
    13981 ttaaagcccc cctgagagga agcttcccag gaagaaaaga tctggcaaag ggctacaatc
    14041 ctcaagtaat tcattccagc tgatagggca taggggtcat ctttaataaa gagtcccata
    14101 agttgaccac taaaaaaata aagtgcaagc agggcaacaa aaccaattac tgaggctatt
    14161 ttggttcctt tatggtagcc ttctagcatc ctttcataat tctcagctcc atagttttgc
    14221 gcaacaaaaa ctcccaaggt tgttgcaagc ccaccaaaca tcatccatcc aatggcctca
    14281 atttgtaccc ccacctcaaa ggctgcaaag gcacccgagc taaaggccgt aacctgtcgt
    14341 gaaatgacag tggagaccga tacgaaaaat atattataaa cagcagcagg ataaccccag
    14401 gataaaattc tttttacctt atcaaaacaa aaatccttta gaaaattgat gtttgcaaga
    14461 agtccttctt tttttcgtat caaaaaataa atcaagctaa cggaaaaatt agctagggca
    14521 gtcgctaggg ctgctccttg aattcccata tcaagactaa aaataaaaat gggatccaag
    14581 accatattta gtataaggcc aacagaatta acaataaaag gtgttttgc
//
LOCUS       DASRNA010000018        10482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_378985,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000018 DASRNA010000000
VERSION     DASRNA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378985"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..780)
                     /locus_tag="VFD08_00485"
     CDS             complement(112..780)
                     /locus_tag="VFD08_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZK09519.1"
                     /translation="MKIYFSSQKGKKRQEDQIKIVEEAAKKHQEADLLLFGQAFLSGD
                     QALSFDYKKDIWKASGLYSQEILDLRKIAKENKIALGFGFYENQKGGIYHSYLIIDKE
                     GESLFKHQALGRDWMEKGACADYRLGHSLGYFELKGKKISLLLGQDFFYDDCLLALCE
                     LDDLVDCFIWLAHKKDPRAKERSQILAKPIYYLTSQGVFLFKQGKLLEKSSEGENFFR
                     SKLG"
     gene            complement(859..1422)
                     /locus_tag="VFD08_00490"
     CDS             complement(859..1422)
                     /locus_tag="VFD08_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK09520.1"
                     /translation="MTREEAVKILKEYNSTESLLQHAYGVEAVMRYFARKEGEDENYW
                     GNIGLLHDLDYELYPDQHCIKTEEILKEKGVEKDFIRSILSHGYGICTDVKPEHYMEK
                     VLYASDELTGLIKATCLVRPSRSILDLKPKSVTKKWKDKAFAAGVDRKTLERGLEMLG
                     LERAELIQECINAMKEVAEEIGLKGNL"
     gene            complement(1450..2097)
                     /locus_tag="VFD08_00495"
     CDS             complement(1450..2097)
                     /locus_tag="VFD08_00495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HZK09521.1"
                     /translation="MARLNVNPTRMELSKLKDRLRTATRGHKLLKDKQDELMRQFIEL
                     IRQNKVLREQVEKKLLESFSKFRITAAMMSPQMLEVALTLPKQSISLEVEERNVMSVR
                     IPEMSFKRSQAAGGLYPYGYVSTPAELDEALRELDDVMEELLELAKIEKTCQLMADEI
                     EKTRRRVNALEYRTIPDLEETIKYIRMKLDENERATITRLMKVKELIAKDEQEAY"
     gene            complement(2097..3479)
                     /locus_tag="VFD08_00500"
     CDS             complement(2097..3479)
                     /locus_tag="VFD08_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005968769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZK09522.1"
                     /translation="MLKEYKTIVEAVGPLLIVEGVEGVKYEELVDVRLQTGEHRRGRV
                     LEIDRDRAMVQLFEGSSGINIKDTSVRFLGRPLELGVSEDMIGRIFDGMGSPIDDGPD
                     IIPEKRLDISGEAMNPAARDYPSEFIQTGISCIDGLNTLVRGQKLPIFSASGLPHNNV
                     AAQIARQAKVLGSDDTFAVVFAAMGITFEEAQFFMDDFRRTGALERAVLFMNLADDPA
                     IERVATPKMALTAAEYLAYEKGMHVLVILTDMTNYAEALREISAARKEVPGRRGYPGY
                     LYTDLAGIYERAGRIRGLNGSITQIPILTMPEDDITHPIPDLTGYITEGQIILSRELY
                     KRGIEPPINIVPSLSRLKDKGIGDGKTREDHADTMNQIYAGYTAGIEARELAVILGDS
                     ALSDADIAFSKFAEAFDERYVDQGYYTDRSIEETLDLGWDLLRVIPRLELKRVRDEYI
                     EKYLDAKGVK"
     gene            complement(3472..5239)
                     /locus_tag="VFD08_00505"
                     /pseudo
     CDS             complement(3472..5239)
                     /locus_tag="VFD08_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807311.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
     gene            complement(5257..5565)
                     /locus_tag="VFD08_00510"
     CDS             complement(5257..5565)
                     /locus_tag="VFD08_00510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005904480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HZK09523.1"
                     /translation="MYRVAILGDRESVLAFQALGLEIYSPRGADETRDTLDRLGSEKV
                     GVIFITEQLAALIPETVKRYDESLTPAVILIPSGAGSLGIGMEKINRNVEKAVGSNIL
                     "
     gene            complement(5558..6547)
                     /locus_tag="VFD08_00515"
     CDS             complement(5558..6547)
                     /locus_tag="VFD08_00515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HZK09524.1"
                     /translation="MDRQDYIYASARIRVFEKNLLKKEQLHRLAQAGEEETFLHYLQE
                     TDYGKYLQNIKEPEAFHKAMDENWQYNLSEVLEMSKDPEVIEILSAKYVYHNYKVLVK
                     EKILGQDLSPLYILVPGMDVEGIRNSLAREEETELANFIYQALEDYEENKDAQRIDLI
                     LDKAYYEDILAKAIKLGDPLFIDFVQYSLDFSNAKALVRLKAQGLEYEALEGVFLPGG
                     HIDSESFKRLYKEEPGVILEGLGKLDPSSTLKKAKLVYEDTGSLASVERLMEDAIMNL
                     VRDGRRITYGPEVLFGYLLAKEKEIMNLRMIFTSLVAGIAPERIEERLRDSYV"
     gene            complement(6550..7101)
                     /locus_tag="VFD08_00520"
     CDS             complement(6550..7101)
                     /locus_tag="VFD08_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HZK09525.1"
                     /translation="MSSLERITGKILEEAQKEIDLLTESLEKQKAQILSDGKKRALAQ
                     GEELIERAKKDAQLDKERSIASAKLKSRDDILSKRISILDRCFDQAKLALSNLSDERY
                     LAFVSQSLKSLDLEGDEKLIVPEDRRALVKGLLPLAKESCEAGFIIQKEGIRYNFQFE
                     ELVDHHREDIQDEIYKLLFSRKE"
     gene            complement(7111..7584)
                     /locus_tag="VFD08_00525"
     CDS             complement(7111..7584)
                     /locus_tag="VFD08_00525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HZK09526.1"
                     /translation="MMDFLVNYGGGLMALFGAFLAVGLSGIGSAAGVGISGEAATGIV
                     VEEPEKFAKSLILQLLPGTQGLYGFVIAILVMLRVEPGMAFQEGFRIFFSCLPIAFVG
                     WKSAVAQAKVAAAGMNILAKNEDHFFKGVIFSVMVETYAILAFVVSIMLLFTTAV"
     gene            complement(7581..9533)
                     /locus_tag="VFD08_00530"
     CDS             complement(7581..9533)
                     /locus_tag="VFD08_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HZK09527.1"
                     /translation="MAIVDMSVFTLISPVTKRDELLKELQKFEYVHFKDMKELDEELM
                     GNQDASSEAALEESRSRIAFLLNQLTPYDSRPKGIKANLIERPSFSLDGLQEKKEKIK
                     EEELYNQVKTLSDAMDEIKQRVTLLETRIEEITPWKELDVKLALVKDTEATKVQLGSL
                     PRRFEESLMSALLDYPDVYFKRVAEEGGQVYGYILYLKEEKEGVEDLLRAHGFNQQLI
                     KGEDTPRLEKKRLVEELEEVKKLQEEKEEDFRELALELEKVEVLFEYYENKKLIYRAT
                     ENFVGTTHMDIISGYVPTARIAELEASIQKCLDKEYYLETQAADYEGDIPVLLENKSV
                     ADAFSGITTMYAMPRYNEIDPTPLLAPFYWIFFGMMGADVGYGLLLLLLSGLVLKIVP
                     LQEGMKKNLKFFFYLSISLTIWGFIFGSFFGDLIPMKGLIDTNKDFTQLLILSLILGM
                     IHLFFGLGIKAFMLIRDKQYMDAIYDVLFWVMAIMGGIVFLLALFVGLDEMIKNISKW
                     VMIIGMAGIVIFGGREAESIGGRIGSGFYELYGISGYVGDFVSYSRLMALGLSGGFIA
                     LSVNIIVNMVIGKGIIGLVGGIIIFVVFHLFNIFLSMLSAYVHTSRLIYVEFFGKFYE
                     GGGKAFKLFKTQPKYINIKNTQGGKL"
     gene            complement(9535..9840)
                     /locus_tag="VFD08_00535"
     CDS             complement(9535..9840)
                     /locus_tag="VFD08_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09528.1"
                     /translation="MNAIEKVKQAEEQALELIEKAKEKALDDLEFSRDYVKREEEQIL
                     AQAQEERELIFQRSRIDKQRRIDRIGEETDRAIEKLDAIDDKFIEPAVDRILERILE"
     gene            complement(9845..10285)
                     /gene="rpiB"
                     /locus_tag="VFD08_00540"
     CDS             complement(9845..10285)
                     /gene="rpiB"
                     /locus_tag="VFD08_00540"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZK09529.1"
                     /translation="MKIVVASDHGGFDLKESIRLYLRDLGHEVLDQGCFDHRSVDYPD
                     YGALAANKVLEEDCLGIIFCGTGIGISLAANKIRGIRAALCHEEYSASMARRHNNANI
                     LALGGRTIGIDLAKSIVDAFLQAEFEGGRHQRRVDKILKLEEGR"
     gene            complement(10282..>10482)
                     /locus_tag="VFD08_00545"
     CDS             complement(10282..>10482)
                     /locus_tag="VFD08_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HZK09530.1"
                     /translation="KDKKCYLFYPEGDLDYAAREYFRLLRLLDMKSYDIIYISEIPSL
                     GIGRALMDRIMRSSGGKARRLL"
BASE COUNT         2777 a         2515 c         1934 g         3256 t
ORIGIN      
        1 attctatcac ttttttacca taaataaaag aagagaaaga gttacttccc ttcaatcatt
       61 tagttgaact ttcttcgttt tagtctatat cctctatttc taggccttat cctagcctag
      121 cttagagcgg aaaaagtttt ctccctcaga agatttttct aagagctttc cctgcttaaa
      181 taaaaagact ccctggctgg taagatagta gatgggtttt gccagtattt ggctgcgctc
      241 ttttgccctg ggatcttttt tatgggcaag ccagataaag cagtccacca gatcatccag
      301 ttcacatagg gctaaaagac aatcgtcata aaaaaaatct tggcccagca gtagagaaat
      361 cttcttgccc tttaactcaa aatatcccag gctatggcct agcctataat cagcacaggc
      421 tcctttttcc atccagtccc taccaagtgc ctggtgttta aaaagacttt ctccctcttt
      481 atcgataata aggtagctat gataaatccc ccccttttgg ttttcataaa agccaaaacc
      541 cagggcaatt ttgttttctt tggctatttt tcgcaggtca agaatttcct gggaataaag
      601 tccagatgcc ttccaaatgt cttttttata atcaaagcta agggcctggt caccagaaag
      661 aaaggcttgg ccaaaaagaa gaagatctgc ctcctggtgt tttttagccg cttcttctac
      721 tatctttatc tggtcctctt gcctcttttt tcctttttga gaagaaaaat atattttcat
      781 ttaaagccta ctttcttttt ttctttcttg aaagttatcc tggctgcttt taataaaaaa
      841 agtgaggcta gctcactttt ataggtttcc ttttagtcca atttcttctg ctacttcttt
      901 catggcatta atgcattcct ggatcagctc tgctctttct aggcccagca tttcaaggcc
      961 tctttctagg gtcttacgat ctactcctgc agcaaaggcc ttgtccttcc acttttttgt
     1021 tacgcttttt ggtttaagat ccaaaatgga gcgggagggt cttacaagac aggtggcctt
     1081 gattagacct gttagctcat cactggcata aaggaccttt tccatatagt gctctggctt
     1141 aacatcggtg cagatcccgt agccatggct taaaatggag cggataaaat ctttctccac
     1201 acccttttcc tttaggattt cttctgtctt gatacagtgc tggtcagggt aaagttcata
     1261 atccagatca tgaagaagtc cgatatttcc ccagtagttt tcgtcctcgc cttctttacg
     1321 ggcaaaatag cgcataacag cttctacccc ataggcatgc tgcaagaggc tttctgtgga
     1381 attatattct tttagtattt ttactgcttc ttctcgcgtc attgtctctc cttgtaagaa
     1441 acaggaaacc taataggctt cctgttcatc tttagcaata agttccttga ccttcattaa
     1501 acgggtaatg gtggcccgct cattttcatc aagtttcata cgaatatact tgatggtttc
     1561 ttctagatca ggaatggtcc gatattctag cgcattaacc cggcgcctag ttttttcaat
     1621 ctcatcagcc ataagttgac aagtcttttc tatttttgct agctccaaga gctcttccat
     1681 aacatcatca agttctctaa gggcttcatc aagttctgct ggggtactca cataaccata
     1741 gggataaagt ccaccggctg cttgggagcg cttaaaggac atctctggaa ttcgaacact
     1801 cattacattg cgttcttcca cctcaagaga aatgctttgt tttggaaggg ttaaggcgac
     1861 ttctagcatc tgggggctca tcattgccgc agtaatgcga aatttcgaaa aggattccag
     1921 gagctttttt tccacttgct cgcgcagcac tttattctgg cgaatcaatt caataaactg
     1981 acgcatcagt tcgtcctgtt tgtcctttaa gagcttgtga ccccgggtag ctgtacgaag
     2041 tctatctttt aacttgctca gctccattcg ggtagggttc acattgaggc gggccattat
     2101 ttcacccctt tagcgtccaa atatttctcg atatattcat cgcgaacacg cttaagctct
     2161 aagcgtggaa tgacccttag gaggtcccag cctagatcga gtgtctcttc aatgcttcgg
     2221 tccgtataat agccttggtc tacatagcgc tcatcaaagg cttctgcaaa ttttgaaaaa
     2281 gctatatctg catcgcttag ggcgctatca cctaggatta cggccagttc ccttgcttca
     2341 atacccgctg tataaccagc ataaatctgg ttcattgtat cagcatggtc ttctcttgtt
     2401 ttgccatcgc ctattccctt atccttaagt cttgataggg agggaacgat atttatagga
     2461 ggttcaattc ctcttttgta aagctccctg gataggataa tctgtccctc ggtgatataa
     2521 cctgtaaggt caggaattgg atgggtaata tcatcctcag gcatggttaa gatgggaatt
     2581 tgtgtaatgg aaccatttag gcctcggatc cgtcccgctc tttcatagat tccagccagg
     2641 tcggtgtata gatatcctgg atacccacgt cggccgggaa cctctttacg ggcggcagat
     2701 atctcacgaa gagcctctgc atagtttgtc atatctgtta ggataacaag aacgtgcatg
     2761 cctttttcat aggctagata ttctgctgct gttagggcca tttttggcgt tgctacccgc
     2821 tcaatagccg ggtcatcagc cagattcata aaaaggacgg cccgctcaag agcgcctgtt
     2881 cttctaaagt catccataaa aaactgggct tcttcaaagg tgattcccat ggctgcaaat
     2941 accacagcaa aggtgtcatc gcttcctaga accttggcct gtctggcaat ttgtgcagca
     3001 acattattat gtggaagccc tgaagcagag aatatgggta gtttttgtcc ccttactagg
     3061 gtatttagtc catcaatgca gctaattccg gtttgaataa actcactggg atagtctcgc
     3121 gcagcgggat tcattgcttc cccactaata tcaaggcgtt tttcaggaat aatatcaggc
     3181 ccatcatcta tggggctgcc cattccatca aaaatccgtc caatcatatc ttctgaaaca
     3241 cccagttcca ggggtcggcc aagaaaacgc accgatgtat ccttaatatt aatcccacta
     3301 gaaccttcaa aaagctggac catggcccta tctctatcaa tttcaagaac tcttccccgc
     3361 ctatgttctc cggtttgcag gcgaacatct actagttctt cgtattttac gccttcaaca
     3421 ccttctacaa taagcagggg gcccaccgct tctacaatgg ttttatattc tttaagcatt
     3481 atttcctcct cgtccaaggg cagatacgtc ttttatcatc cgctctttaa gagcatccaa
     3541 ggaatcgagt ttttcgccgg ttactagctt catcctggcc atgggctcgc gaacctcaag
     3601 ctttagaatc tcatccaaat aggctcccga atccattgct ttttgggatt cctcatagaa
     3661 agttaaaatg atatccagca ttttatactg tttttccaag gaacaatagg tgtccacatc
     3721 gtggaaagca ttttgttgga ggaagtcttc tcttaggctt ttagagactt ctagttttag
     3781 ctggtcagct tcactaaggg agtctcgccc aacaagacga acaatctctt gtagagcact
     3841 ttcttcttgt aaaagtgcca tggaatcttt tctatatttt gaaaagttct tactaatatt
     3901 ttcatcaaag aattcatcca ttttttcttg atacaagcta taagagttga gccagttgat
     3961 agcagggaag tgacgagaat aacttagggc atagtcaagt ccccagaata cttttacaat
     4021 tcgcagggtt gactggctaa ctggctcgga aatatctcct cctggaggtg aaaccgctcc
     4081 aattactgtt agggctcctt ctcttggctg gctaccttgg cttagtacct taccggctct
     4141 ttcatagaag tcagctaccc ggctggctaa ataagcagga taaccctcat caccaggcat
     4201 ctcttccaga cgaccactca tctccctaag ggcttcagcc cagcgggaag tagagtctgc
     4261 cattagggct acactataac ccatatctct aaagtactct gctatggtga ttcctgtata
     4321 gatggaggcc tccctagcag caacaggcat attggaagta ttggcaataa gcactgttct
     4381 tttcattagg gactcgcccg tacctgggtc aattagttcg gggaattcca taagtacgtc
     4441 ggtcatctca tttccacgtt caccacatcc aacataaaca atgatttcag cgtcggccca
     4501 cttggcaagc tggtgctgaa ccactgtttt tcccgaacca aaggggccag gaattgcggc
     4561 ggttcctccc cttagcaaca gggaaaaagg tatctattac tctttgacca gttactagag
     4621 gaatactagg atctagtttc ctatcatagg ggcgacctct acgtacaggc cactcctgga
     4681 tcatatcaag ttcaaatatc tcaccatctt tttcaattac tactactttt tcttttacgg
     4741 tgaaacttcc gcttttaatt tcttttatcc taccaccctt tccagggggt accataattt
     4801 tatggacaac gacagggttt tcctggaccg ttgccaggat atctccttca gtgacctcat
     4861 ctccctcttt gacaagggga acaaagtccc atttcttatc tcgatccagc gccacaactg
     4921 cactccctct ttttaagaag tttccttcta aagagcgcat aacagctagg ggacgctgaa
     4981 tcccatcaaa catctgttca attagaccag ggccaagttc cacagaaagt ggatggccag
     5041 tggtataaac cacttctcct ggaccgatgc cgatggtttc ctcataaact tgaatggagg
     5101 ccctatctcc tcgcatttcg ataatctctc caattagtcg attttcactt acgaggacca
     5161 catcataaac attggcttct tccatgccct cggccacaac aagtgggccg gagaccttga
     5221 ttacttttcc tgttttcaaa ttttcgtcct caccttctaa agaatatttg acccgactgc
     5281 tttttccaca tttcgattga ttttttccat gccaatgcct agactaccag caccacttgg
     5341 aataagaatt acggccgggg ttaaactttc gtcataacgt tttacagttt ctggaattag
     5401 agctgcgagt tgttcggtaa taaagattac ccctaccttt tcactgccca aacgatccag
     5461 agtatccctg gtttcgtcgg cgcctcgggg gctgtatatt tctagaccca gcgcctgaaa
     5521 ggcaagtacc gattcccgat ctcccaggat ggcgactcta tacataacta tcacgcagcc
     5581 tttcttctat ccgctctggg gcaatacctg ccaccaaaga agtaaatatc atacgtaggt
     5641 tcataatctc tttttccttg gctaaaagat aaccaaagag tacctcaggg ccgtaggtta
     5701 ttctacgtcc atcgcggacc aggttcataa tagcgtcttc cattagtctt tctacactag
     5761 ctaaagaccc agtatcttca tagacaagct tggctttttt aagagtagag ctaggatcaa
     5821 gtttacccag gccctctagt ataacgccag gctcctcctt ataaagtctt ttaaaggact
     5881 cgctatctat atgacctcct ggaagaaaaa ctccttctag ggcttcatat tctagtcctt
     5941 gggctttaag tctgactagg gcctttgcat tgctaaagtc caaactgtat tggacaaaat
     6001 caataaaaag tggatcccca agcttgatgg ccttggccag tatatcttca taataggcct
     6061 tatctaagat tagatcgatg cgctgggcat ctttattttc ttcataatct tctagggcct
     6121 ggtagataaa gttagctagt tcagtttctt cttccctggc aagactgtta cgtattcctt
     6181 ccacatccat tccaggaacg aggatataga ggggagaaag gtcttgcccc agtatttttt
     6241 cttttaccaa aaccttgtag ttatggtaaa catacttggc acttaaaatc tcaattacct
     6301 cagggtcctt actcatttct agcacttcac ttagattgta ctgccaattc tcatccatag
     6361 ctttatggaa ggcttctggt tcctttatat tttgaagata cttgccataa tctgtttcct
     6421 gaagataatg taaaaatgtt tcttcctccc cagcctgggc caggcggtgg agctgttctt
     6481 tttttaggag attcttttca aagaccctta ttcttgctga tgcataaata tagtcttgtc
     6541 tatccatgtc tactcctttc ttgaaaagag cagtttatag atttcgtctt gaatatcttc
     6601 tctatgatga tctacaagtt cttcaaattg aaaattgtag cggatgcctt ctttttgaat
     6661 aataaagccc gcttcacaac tttccttcgc aagaggtaga agacctttta ccaaggccct
     6721 tctatcttcg ggaacgataa gtttctcatc gccttcaagg tccaaggact ttagtgactg
     6781 gctaacaaag gcaaggtagc gctcatcgct taaattgctt agggccagct tggcctgatc
     6841 aaagcaccga tcaagaatag atatgcgttt acttaaaata tcatcgcgac tttttagctt
     6901 tgccgaggca atgctacgct ccttatcaag ttgggcgtct tttttcgctc tttctatcag
     6961 ttcttctccc tgggcaagag ctctcttttt tccatcggaa agaatttgcg ctttttgctt
     7021 ttccagagac tctgtcaata aatcaatttc cttttgtgct tcctcaagta tttttcctgt
     7081 tattctctct aaactagaca tagcagccac ctatacggcg gtagtgaaca gtagcatgat
     7141 ggaaacaaca aaggcaagaa tcgcataagt ttctaccata actgagaaaa taacaccctt
     7201 gaaaaagtga tcttcattct tggctagaat attcattcca gcggcggcta ccttggcttg
     7261 tgcaacggct gatttccaac caacaaaagc aatgggaagg caggagaaaa atattctgaa
     7321 accttcttga aaggccattc ctggttcaac acgtaacata accagaatag caataacaaa
     7381 tccatatagt ccttgtgtac caggtaacaa ctgaagaatt agagactttg caaatttttc
     7441 tggttcttcc actacaatac ctgtggctgc ttcaccagaa atcccaacac cggcagctga
     7501 gccgattcct gaaagaccaa ctgccaaaaa tgctccaaat agggccatca gtccgccacc
     7561 atagttcact aaaaaatcca tcataatttt cctccttgag tgtttttaat attgatgtat
     7621 tttggttgtg ttttgaaaag tttaaaggct tttccaccgc cttcgtaaaa tttcccaaag
     7681 aattcaacat agatcagcct actggtgtgt acataagctg aaagcatact tagaaaaata
     7741 ttaaagagat ggaatacaac aaagataata attcctccaa ccaaaccaat tattcccttg
     7801 ccgattacca tatttacaat gatatttaca gatagggcaa taaagccccc actaaggcct
     7861 agggccatca agcgagaata agagacaaag tctcccacgt aacctgaaat accataaagt
     7921 tcataaaatc ctgaaccaat tcttccaccg atactctcgg cctctcgacc accgaaaatt
     7981 acaataccgg ccattcctat aatcatcacc catttgctta tattttttat catttcatca
     8041 aggccaacaa acagggccaa taaaaataca attcctccca taatagccat aacccagaag
     8101 agtacatcat agattgcatc catatactgc ttgtctcgga tcaacataaa ggctttgatg
     8161 ccaaggccaa aaaacagatg aatcattccc aggataagac ttaaaatcag gagctgggtg
     8221 aagtctttat ttgtatcaat tagtcccttc atagggatca aatctccaaa aaaggaccca
     8281 aaaataaatc cccaaatggt taaactaata cttagataaa agaagaactt tagattcttc
     8341 ttcattccct cttgcagagg tacaattttt agtaccaagc cagataggag taaaagtagc
     8401 agtccataac ctacatcagc gcccatcata ccaaagaaaa tccagtaaaa gggggctagc
     8461 aggggggtag gatcgatttc attatagcga ggcatagcat acatggttgt tataccgcta
     8521 aaagcgtcgg ccactgattt gttttccaga agaactggta tatctccctc ataatcagct
     8581 gcttgtgttt caagataata ttctttatca agacattttt ggatgctggc ttccagttct
     8641 gctatacgtg ccgtaggaac ataaccactg ataatatcca tatgggtggt tcctacaaag
     8701 ttttctgtcg ccctgtaaat tagttttttg ttttcataat attcaaaaag aacttcaact
     8761 ttttctagtt ccagagctag ctctcgaaag tcctcctctt tttcttcttg gagttttttg
     8821 acttcctcga gttcctcgac tagtctcttt ttttctagcc tgggtgtatc ttctccctta
     8881 atcagctgct gattaaagcc atgagctcgt agaagatctt ctacaccctc tttttcttct
     8941 tttagataaa ggatataacc ataaacttgt ccgccttctt cagccaccct tttaaaatac
     9001 acatctggat aatccagtag tgcactcatt aggctttctt caaaacggcg ggggaggctt
     9061 cctagttgaa cctttgttgc ctctgtatcc ttaacaagag caagcttaac atctagctct
     9121 ttccaaggag taatttcttc tatccttgtt tctaaaagag tgaccctttg tttgatttca
     9181 tccatggcat cactaagtgt tttaacctga ttgtagagct cttcttcctt gattttctct
     9241 tttttctcct ggagaccatc aaggctaaaa ctagggcgct caataagatt ggctttaatc
     9301 cccttgggcc ggctgtcgta aggagtcagc tggtttagaa gaaaggcaat acgagacctg
     9361 ctttcttcta gggccgcctc actgctggca tcctggtttc ccataagctc ttcatcaagc
     9421 tctttcatat ctttaaaatg tacatattca aacttttgaa gttccttaag taattcatcc
     9481 ctctttgtca cagggctaat gagggtgaaa acactcatat caacaattgc catcttattc
     9541 tagtatcctc tccaggatgc gatctactgc aggttcaatg aacttatcat caatagcatc
     9601 cagtttctcg atagcacgat ctgtttcttc cccaatgcga tctattcgtc tttgtttgtc
     9661 aatgcgggag cgctgaaaaa ttaactccct ttcctcttgg gcttgggcaa ggatttgttc
     9721 ttcttctctc ttgacataat ctcttgaaaa ctcaagatca tcaagagcct tttcttttgc
     9781 cttttctatc agctctaagg cctgctcttc agcttgttta actttttcta tggcattcaa
     9841 cgctttacct cccttcctcg agctttagta tcttgtcaac gcgacgctgg tgacgacccc
     9901 cctcaaattc tgcctgtaaa aaggcatcta caatactctt tgcaaggtca atcccaatag
     9961 ttctaccacc taaggcaagt atattggcat tgttgtggcg ccgggccatg gaggcagaat
    10021 actcttcatg acaaagagcg gctctgattc cacgaatctt attggccgcc aaacttatcc
    10081 ctatacctgt tccacaaaaa ataataccaa ggcaatcctc ctcgaggact ttattggctg
    10141 caagagctcc ataatcggga taatcaacac ttctgtggtc aaagcacccc tggtcaagta
    10201 cctcatggcc caagtccctc aaataaagtc ttatactttc tttgagatca aagccgccat
    10261 ggtctgatgc aacaacaatt ttcacagtaa cctccttgct ttgcctccac ttgaacgcat
    10321 gatgcgatcc atgagtgccc gacctattcc tagactagga atctcactta tatagattat
    10381 atcataactt ttcatatcta gtaaccttaa tagtcggaaa tattctctag cagcatagtc
    10441 taagtcccct tcaggataaa ataagtaaca tttcttgtcc tt
//
LOCUS       DASRNA010000019         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_601767,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000019 DASRNA010000000
VERSION     DASRNA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601767"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..614
                     /locus_tag="VFD08_00550"
     CDS             54..614
                     /locus_tag="VFD08_00550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZK09531.1"
                     /translation="MKKIGIIIGSLRKKSYARKWAENMLELYPKDFEVEIIEVGELPF
                     YNEEFDEEGPLPGAVKAFREELEMIDGFLFVTSEYNRSIPPALKNALDIGSRPYASTK
                     WAKKPALIVSHSISKLGGFGANHHLRQVLAFLDMPTVGQPEIYLGNSSQAFDEKGKII
                     DSSTIELLLKAVDSHVDLVRRFEMKR"
     gene            complement(653..1492)
                     /locus_tag="VFD08_00555"
     CDS             complement(653..1492)
                     /locus_tag="VFD08_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZK09532.1"
                     /translation="MKRKIIEKTKGFRTTDGAGVSLVRVLGNTTVKSYDPILMLDSFD
                     SLDPADYEKGFPLHPHRGIETISLISKGNMVHKDSLGNEDAITDGEVQWMTAGSGIMH
                     EEALPASERMLGVQLWLNMAKKDKMAPPEYQSIKKEEIEEIPLEDGTLRLISGSYKDH
                     QGFMGKYQALDYYHILLEAGGSLDLEMPREKSVLVFLLLGDARVAGEVVEEKTAVKLS
                     EGTQLLIEALDQPIEILFISSDRLDEPVAWGGPIVMNTRAELNQAFKELQEGSFLKEK
                     IHY"
     gene            1756..3015
                     /gene="hutI"
                     /locus_tag="VFD08_00560"
     CDS             1756..3015
                     /gene="hutI"
                     /locus_tag="VFD08_00560"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZK09533.1"
                     /translation="MKASLVIEGIDNLITLRGENKARYGKAQKEIGLIKSGLVALDGE
                     RILYVGEGELPSSISTDEDTVFIDGRGKLVTPGLIDPHTHLVHGGSREDEFKLKLEGV
                     DYLDILAAGGGIHSTVEATRKASFDQLYLQAKKSLDRMLSYGVTTVEAKSGYGIGDFS
                     TEVKQMEVALKLGEDHPIDLVSTFMGAHAIPKEYSHAPDDFVDIIIDQMLPAVARKNL
                     AVFNDVFCEEGVFTIDQSRKILLAGKELGLRPKLHADEIVPLGGAELAAEVGCISAEH
                     LMAVSDQGIKDLAKKKVVAVLLPGTSFNMQKGTAAPARKMIESGLPIALSTDYNPGSC
                     PSENLQFMMSLGSLLLKMTPDEVITAVTINAAAALGLEEEKGSLEVGKRADIAIFDVP
                     NLAYMLYHFGVNHTDKVIKDGKIVFEK"
     gene            3025..>3740
                     /locus_tag="VFD08_00565"
     CDS             3025..>3740
                     /locus_tag="VFD08_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZK09534.1"
                     /translation="MSSNKMIEEAMEIKLDKALPSYPDFAPGIRRAPKRGAELSQRDI
                     VLALKNALRYIPEELHKEVAPEFLDELLSRGRIYGYRFRPNRKIYGKALEQYKGNCTE
                     GKAFQVMIDNNLSFDVALYPYELVTYGETGQVFQNWMQYNLTMKYLEILTDEQTLVLE
                     SGHPVGLFHSPKSAPRVIITNALMIGAFDDQKNWERAQALGVANYGQMTAGGWMYIGP
                     QGIVHGTYNTILNAARLKLGV"
BASE COUNT         1124 a          747 c          822 g         1047 t
ORIGIN      
        1 atctatagga aagatacttg atttttctgt tgaataaata aaggagtaaa ccaatgaaaa
       61 aaataggaat tattatagga agcctgcgca agaaatccta cgcccgcaaa tgggctgaga
      121 atatgttgga actttatcca aaggactttg aagtagagat tattgaagta ggagagttac
      181 ccttttataa tgaagagttt gatgaagagg gacctctgcc aggagcggta aaagccttta
      241 gagaagaact ggaaatgata gatggttttc tttttgtcac ctcagaatac aatcgctcca
      301 tcccaccagc actaaaaaat gccctagata taggatcaag accctatgcc agtacaaagt
      361 gggcaaaaaa gcctgccctt attgtatccc actcgatttc taagctggga ggctttgggg
      421 ccaaccatca tttaagacag gttctagcct ttcttgatat gcctacggta ggacagccag
      481 aaatctacct tggaaattca tcacaagcct ttgatgaaaa aggaaagatt atcgatagtt
      541 caacgataga gcttctatta aaggcagttg attctcatgt tgaccttgta agacgatttg
      601 aaatgaaaag ataaaaaaag cagaacgcgc tctgcttttt tctttgcaag aattagtagt
      661 ggattttttc ttttaaaaaa cttccctctt gcagttcttt aaaggcctgg tttaattctg
      721 cccttgtatt cataacaatg gggccacccc aggcaacagg ctcatcaaga cggtctgaac
      781 taataaagag gatttctatt ggctgatcaa gtgcctctat taaaagctgg gtgccctcag
      841 agagcttaac tgctgttttt tcctctacca cttctcctgc caccctggca tctcccagga
      901 gtaaaaaaac aagaacggac ttttcccttg gcatttctaa atcaagagat ccccctgctt
      961 ctagaagtat atgatagtaa tcaagagcct ggtattttcc cataaaaccc tggtggtcct
     1021 tatagcttcc gcttataaga cgaagagttc catcttctaa ggggatttct tcgatctctt
     1081 cttttttgat gctctggtac tctggtggtg ccattttatc tttttttgcc atattgagcc
     1141 aaagttgaac ccccagcata cgctcagaag ccggaagggc ctcttcatgc ataatgcctg
     1201 aacctgctgt catccactga acttctccat ctgtaatggc atcttcattg ccaaggctat
     1261 ccttgtgaac catattaccc tttgaaataa ggctaatggt ttcaattccc ctatggggat
     1321 gaaggggaaa gcctttttca taatcagctg gatctaggct atcaaaggag tctagcatta
     1381 agatgggatc atagcttttt actgttgtat tacctagaac tcttaccaga cttacgcctg
     1441 ccccatcggt ggttctaaag ccttttgttt tttctattat ttttcttttc atatatactt
     1501 tcctttctct taagtctagt cttcttatac ccctgttttc ctttttctat tcttattttt
     1561 ttgccaagta aaaatcaaaa ataagaatat agagggtaag taaagaagaa aggatggcta
     1621 ggtccagcag aaattgattt tctttgtgta ttttgagaaa ttactcataa aatctagtat
     1681 aataaaatag tgtaggttac cacttgtttt aaattgcatt taaaacaaag atcgcttcct
     1741 aagaagggag aaattatgaa agcaagtcta gtcattgaag gcatagacaa tctaattacc
     1801 ctgcggggtg aaaataaagc acgttatggt aaggcccaga aagaaatagg cctaatcaag
     1861 tcagggctag tagccctaga tggggagaga atcctctatg tgggagaagg agaacttccc
     1921 tcttctattt ctactgatga agatactgtt tttatcgatg gaaggggcaa gctagtaaca
     1981 ccaggcctaa ttgatcccca tacccacctg gtccatgggg ggagtagaga ggacgaattt
     2041 aagcttaaac tggagggagt tgattatcta gatattttag cagcaggtgg agggatccac
     2101 tccacagtgg aggctactag aaaagcaagt tttgatcagc tctatcttca ggcaaaaaag
     2161 agccttgacc gaatgctctc ctatggggtt accactgtag aggcaaaatc cggctatggc
     2221 attggggatt tttcaacaga agtaaaacag atggaggttg ccctaaaact tggagaggac
     2281 caccccattg atcttgtctc tacttttatg ggggcccacg ccattcccaa ggagtatagt
     2341 catgcgcccg atgactttgt tgatatcatt attgatcaaa tgcttccagc tgtagccagg
     2401 aagaatctgg ctgtgtttaa tgatgtattt tgtgaagagg gcgtttttac catagatcag
     2461 tcccgtaaaa ttcttctagc aggcaaagag ctgggcctaa ggcccaagct ccacgcggat
     2521 gaaattgtcc ctctaggagg ggcagagcta gcagcagaag taggatgcat ctcagcagaa
     2581 caccttatgg ctgtgagtga tcagggaata aaagatttag caaaaaagaa ggtagttgca
     2641 gtccttcttc cagggacaag ttttaatatg caaaaaggca cagcagcccc agctaggaaa
     2701 atgattgaaa gtggtcttcc catcgcccta tcaacagact ataatccagg cagttgtcct
     2761 agtgaaaacc tgcagtttat gatgagtctg ggatcgcttc ttttaaaaat gacccccgat
     2821 gaagtgatta cagctgttac aataaatgct gcagcagccc ttggactgga agaagaaaag
     2881 gggtccctag aagtgggaaa acgggctgat atagcaatct ttgatgtacc aaatcttgcc
     2941 tatatgctct atcattttgg agtaaatcat acggataagg taatcaaaga tggcaagatt
     3001 gtttttgaaa aataggagga aaaaatgtcc agcaataaga tgattgaaga agcaatggaa
     3061 ataaaattag acaaggccct accttcctat ccagactttg ctccgggtat tcgaagggct
     3121 cccaaaagag gggctgaact ttcccagagg gatatagtgc tggccttgaa aaatgccctg
     3181 cgctatatac ctgaagaact ccataaggaa gtggctccgg aatttttaga tgagctccta
     3241 agtaggggga ggatatatgg atatcgcttc cgcccaaata gaaaaattta tggaaaggct
     3301 cttgagcagt ataaaggaaa ttgtactgag ggcaaggcct ttcaagtgat gatagataat
     3361 aatctttcct ttgatgttgc tctttacccc tatgagctag tcacctatgg agaaacagga
     3421 caagttttcc aaaactggat gcagtacaat ttaaccatga aatacctaga aatcttaact
     3481 gatgagcaga cccttgtcct agagtcggga catccagtgg gactcttcca ctctcctaaa
     3541 agtgccccaa gagtcattat aaccaacgcc cttatgattg gggcctttga tgatcaaaag
     3601 aactgggaaa gagcccaggc gcttggtgtt gcaaactatg ggcaaatgac cgcaggaggc
     3661 tggatgtata ttgggcccca gggcattgtt catggaacct ataatacaat ccttaatgca
     3721 gccaggctca agcttggtgt
//
LOCUS       DASRNA010000020         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_379921,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000020 DASRNA010000000
VERSION     DASRNA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379921"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFD08_00570"
     CDS             <1..335
                     /locus_tag="VFD08_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HZK09535.1"
                     /translation="NIMLANNICDRQEDMANGRHTLVSYIGEKKALRLFGISQGLILV
                     VLFLEMMAGLFPWTGAIVFLSAPILYKNTKRLIEKPSKEKTFVFSVKNFVLVASLMTL
                     ALIGALLW"
     gene            329..703
                     /locus_tag="VFD08_00575"
     CDS             329..703
                     /locus_tag="VFD08_00575"
                     /inference="COORDINATES: protein motif:HMM:NF018685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HZK09536.1"
                     /translation="MVKDLRYGDLIIIFFIALVGLAVFFFNSGFFGEESEILEVSKDG
                     QVIAKLLLSEDQLYTTPDGKNTVEIKDGVVSMVEALCPDQLCVHTKPLVNSSRSIVCL
                     PNRVVLRIIGKDEEAQIDAITQ"
     gene            687..1220
                     /locus_tag="VFD08_00580"
     CDS             687..1220
                     /locus_tag="VFD08_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HZK09537.1"
                     /translation="MPSHNKSKLIRLTTMALLVSMAAVLSYFERFIPVITVPGVKLGL
                     ASIFTLAALVLLSWKEALLILIARIFLVSFFAGSPLSLFYSLSGGLLSLFAMYLALKL
                     VPKHISIIGVSLLGAFFHNTGQCIVLAFIIGSWEVALGYYPILVLAALLTGGLTGYIS
                     SYFLQHIQSMPLGPGLS"
     gene            1466..2044
                     /locus_tag="VFD08_00585"
     CDS             1466..2044
                     /locus_tag="VFD08_00585"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZK09538.1"
                     /translation="MKRCKWKNLENHQIYMDYHDKEWGIPSYEDGYLFEMIVLESFQA
                     GLSWLTILNKREAFRLAFDNFDPHLIKDYDESKIKELLADKSIIRNENKIRATINNAL
                     CFIKIQEEFASFSEYIWSFTNHEIVFNPDPNISKNELSERVSRDLKKRGFKYMGSVIS
                     YSYLEAVGIMNNHIPQCFKYSKNKIEEVEKSL"
     gene            2365..>2511
                     /locus_tag="VFD08_00590"
     CDS             2365..>2511
                     /locus_tag="VFD08_00590"
                     /inference="COORDINATES: protein motif:HMM:NF017655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HZK09539.1"
                     /translation="MGKKRNAAIIYFGLISMLIDHIGLLFFPDQLIFRSIGRLAFPIF
                     AWALA"
BASE COUNT          735 a          433 c          528 g          815 t
ORIGIN      
        1 ctaatataat gttagctaac aatatttgcg ataggcaaga agatatggca aatggacgtc
       61 atactctggt aagctatata ggagaaaaaa aggcccttag attatttgga atctcccagg
      121 gactcatcct ggtggtcctt tttttggaaa tgatggcagg actctttccc tggacgggag
      181 ccatcgtctt cttatctgct ccaattctct ataaaaacac caaaagattg atagaaaagc
      241 caagcaaaga aaagaccttt gtattttctg ttaaaaactt tgttcttgtg gcatcactta
      301 tgacactggc actgatagga gccttgcttt ggtaaaagac ttgcgttatg gagaccttat
      361 tattatcttt tttattgccc tagttggtct agctgttttc ttttttaata gcggtttttt
      421 tggagaagaa agtgagatct tagaggtttc caaagatggt caggtgattg caaaactcct
      481 gctctcagag gaccagcttt atacaactcc tgatggaaaa aatacagtag aaataaaaga
      541 cggtgtggtt tctatggtag aggccctatg tccagaccag ctttgtgttc atacaaagcc
      601 cctggtcaat tcttctagat ccattgtttg ccttcctaat cgagtggttc tgcgcattat
      661 aggaaaagac gaggaggcgc aaattgatgc catcacacaa taaatccaaa ctgatccgcc
      721 taacaaccat ggcgctctta gtaagtatgg cagctgtctt aagttatttt gagcgtttta
      781 tccctgtaat tactgttcct ggggtcaagc taggcctggc tagtatcttt acactggcgg
      841 ctttggttct tttgtcttgg aaagaggcac tgcttattct tattgctaga atttttttgg
      901 tgtccttttt tgcaggttca ccccttagtt tgttttatag tttgagcggg ggactcttaa
      961 gtctttttgc tatgtacctg gcgcttaagc ttgtacccaa acatatttcc attattggag
     1021 taagtctctt gggggccttt tttcacaaca caggccagtg tattgtcctg gcttttatta
     1081 ttggaagttg ggaagtggcc ctgggctact atccgattct tgtcctggca gcccttttaa
     1141 cgggaggtct tacgggctat atttcaagtt actttttaca acatattcag tccatgcccc
     1201 tagggccagg cttatcctag ggggctttat tatacttata tcctttagga taaaacagat
     1261 aggagatttt tcttctgtct gtttttaatt tgggcataaa caaaggagcc ttctagtaga
     1321 ctggatggtg ccagaaggct gcagagaact ttccctagct ttgtgataaa atagcttata
     1381 aaggggctat tttaggtttt ttatggctct agaagctggc tttatctttt atcataggaa
     1441 gaaaaaagaa ttgtaagagg gaaatatgaa acgctgtaaa tggaaaaatt tagaaaacca
     1501 tcaaatttat atggattatc atgataagga atggggcatt ccttcctatg aggatggata
     1561 tttattcgaa atgattgtac tagaatcctt tcaagctggt ttatcatggc ttaccatact
     1621 caataagcga gaagctttta gactggcctt tgataatttt gatccccacc ttataaaaga
     1681 ctatgatgaa agtaaaataa aggaactact agctgataaa agcatcattc gaaatgaaaa
     1741 caaaataaga gcaacaatca ataatgctct ttgttttatt aagatacaag aagaatttgc
     1801 tagctttagt gagtatatct ggtcctttac caaccatgaa attgtcttta atcctgatcc
     1861 aaatataagc aaaaacgaat tatcagaaag ggtctcaagg gatttgaaaa aaagaggctt
     1921 taagtatatg ggaagtgtaa taagctattc ctaccttgaa gcggtgggga taatgaacaa
     1981 tcatattcca cagtgcttta aatacagcaa aaacaaaata gaagaggtag aaaaaagcct
     2041 ttaggtctaa ggggagtttt atttttaaat tacagcccac aaaaggggta ataaatcagg
     2101 gatctgaagc ggctggtcct ttagtctgtt tttttcggcg ttaattacta gatttttaat
     2161 tcccctaaag attgccttct tccagtgtgt gaagaaaagt tagtaaagca aagaagaaaa
     2221 aagacgatca attgctaagg cccttctcat gatataatag cctttaagtt gctttatata
     2281 ggggcctaag gaatctaggt atattctttt agattagata aagaagctag tggttttttc
     2341 tatagccttt aacttggggg acccatggga aaaaagagaa atgcagccat tatatatttt
     2401 gggcttataa gtatgctgat agatcatatt gggcttcttt tttttcctga ccagctgatc
     2461 tttcgaagta ttggaagact ggcctttcct atctttgcct gggctttggc c
//
LOCUS       DASRNA010000021        10285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_380132,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000021 DASRNA010000000
VERSION     DASRNA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10285
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380132"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /gene="truB"
                     /locus_tag="VFD08_00595"
     CDS             complement(<1..632)
                     /gene="truB"
                     /locus_tag="VFD08_00595"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZK09540.1"
                     /translation="MWGKINLYKEADMSSQQAVTRLRRLLGIKKIGHSGTLDPMATGV
                     LNCFVSQATKFIDLLPEEVKVYQADFILGQTSDTLDIWGNITSKPFVSPTERELKEEL
                     GKFIGESFQIPPMYSAIKYKGQALYHYARQGIEIPRKKRKININKLELINFDGKKGSI
                     RIHCSRGTYIRTLIDDLGQNLGTGAIMSGLVREHNDWAGLKDCYSLKEIEA"
     gene            complement(607..1560)
                     /locus_tag="VFD08_00600"
     CDS             complement(607..1560)
                     /locus_tag="VFD08_00600"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZK09541.1"
                     /translation="MMNLNKAKVFIEKYHYFIVLPHIKADGDALGSALAVKLILESLG
                     KKAVIWNEDPLQHSLKPFFQGSYNTAKPEGDFDALISVDTADLSRLGERYNHLNHLPL
                     LNIDHHATNSNYGDVNLVYGGLSSTGELIGLLMEELEIPLTNKIADAIYIAIVTDTNR
                     FFYNSTTPSTLKWAAKLLEAGADIEKIHRYIFGEKPLSLLRLLSLATEKIEFIAPNIV
                     YCRLKKEDMQKAGFFETDDVITMIRDIENVDVAALVYDYEDSQKLSLRSKGVQNVDDL
                     AQFFGGGGHLKAAGATISEEDIPSLKRMLKELEYVGQDQLI"
     gene            complement(1544..1903)
                     /gene="rbfA"
                     /locus_tag="VFD08_00605"
     CDS             complement(1544..1903)
                     /gene="rbfA"
                     /locus_tag="VFD08_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZK09542.1"
                     /translation="MNKQRLRKVNEEIRRIISTSLIFEVKDPRLPTMINVLGAETTAD
                     MKQTKIFVSWVGEEDCEEVLLVLNNASGYFRKLLGKQLTTYHTPEPQFFYDNSVEKGM
                     EMDELLRGITYSDDELE"
     gene            complement(1900..4065)
                     /gene="infB"
                     /locus_tag="VFD08_00610"
     CDS             complement(1900..4065)
                     /gene="infB"
                     /locus_tag="VFD08_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZK09543.1"
                     /translation="MRKIRIYELAKEYKMTSKNLIVKLENIGITVSNHMSTLTKEEHD
                     KFIDFVRGDLPTEESSKEIKEPAKEKRDKAEKKTDEKKPSSKKEKESSKKTAEPSKKE
                     TETPATPKRNWQKRKRGTRAQYRKEKEDKTVDLSSSEIEIDDHITLGEFAEKIQKPAT
                     ELIMKFMDMGEMYTINSNISFEDAELLALEYELVITRSQSLSEIEEERFDYEDSKDDL
                     EERAPVVTVMGHVDHGKTSLLDTIRQTRVTKREAGGITQHIGASEIHSKGKKIVFLDT
                     PGHEAFTTLRARGALITDIAILVVAADDGVKPQTVEAIHHAKSAGVPIIVAINKMDKP
                     AADPERVKQDLTEHNLLVEDWGGDVIAVEVSAITGEGIETLLEMVLLVAEMLELKANP
                     ERPGVGTIIEANVDRRMGSTATVLLEKGTIQIGDNVFAGSSYGRVRTMIDDRGKRLKI
                     AGPSSAFKITGLNEVPMAGEKIYVTADDQTARIYASKNADKERSERIHQPKMVASLDT
                     VQGEDGETKVLNIILRADVHGSVEAIKQSLEKINTDEVMVNIIKAQVGAITESDILLA
                     TASEAVILGFNVRPDNNIQNIAERDSVEIRTYRVIYDIINDVRDALSGMLDPDRKEVV
                     EGNIEVRDTFKVPGAGTIAGGFVMRGKISRTSMVRLIRDGIVIHEGEVSSLKRFKDDV
                     RQVQTGYECGIGIKNYNDIKEGDIIETFIIKEIQRSLDS"
     gene            complement(4070..4384)
                     /locus_tag="VFD08_00615"
     CDS             complement(4070..4384)
                     /locus_tag="VFD08_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09544.1"
                     /translation="MNSDKNWENKTYTLLGFAAKSGVLVSGTFAIKRALKEDSLGLLL
                     FSEECSNKTIASFLYHSDIDHIILGPSERMSKTTGVKNRHVFAVKKGKLAENMIKEIS
                     RR"
     gene            complement(4356..4619)
                     /locus_tag="VFD08_00620"
     CDS             complement(4356..4619)
                     /locus_tag="VFD08_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HZK09545.1"
                     /translation="MKKRKTPLRKCVGCGQLKDKRELIRLVRSKEGHVSIDPTGKLNG
                     RGAYLCKDKACFEQALKEKGLQRSFKSMIKQETIDELRQKLGK"
     gene            complement(4606..5706)
                     /gene="nusA"
                     /locus_tag="VFD08_00625"
     CDS             complement(4606..5706)
                     /gene="nusA"
                     /locus_tag="VFD08_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZK09546.1"
                     /translation="MDKITDVIHMIARERDIPEETLFESIEAALVTAYRKNFGKNRQA
                     RVDINRDSGDIKVFSRYNVVEEVEDAELEISLEEAKKINPVYELDDVLEKEETPKDFG
                     RVSALAAKQVVVQRIREAERTSVYDEYKNREQELVYSQIQRINNGVVYFNLGKAEAMM
                     PAKEQIPGERYRVGDRLRSYIIEVKDEKRLSLEAGKNPRDVRGKKGLEIILSRTHPGL
                     VRRLFEMEIPEILEGDVLIKSIAREPGSRTKIAVQAGREGLDPVGACVGPKGSRVAHI
                     VDELGGEKIDIVEYTDDLPKFIENSLSPAKVSEVRVLEERSAKVIVPDFQLSLAIGKE
                     GQNARLAAKLTGIRIDILSESQNLESKNYEKA"
     gene            complement(5715..6176)
                     /gene="rimP"
                     /locus_tag="VFD08_00630"
     CDS             complement(5715..6176)
                     /gene="rimP"
                     /locus_tag="VFD08_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZK09547.1"
                     /translation="MKSISKVAEEILTPFLESKDLELYDLAFVKEGPHHYLRVFIDSE
                     KGISLNECEEVSQFLSKALDKKDPFKKQYFLEVSSPGIERTLKKDDHYRRFIGHSIQL
                     SLFSKFQDQKVINGKLLSKDNKYIKILDDITGKELEVPVDLVAKARTNIEF"
     gene            complement(6285..>10285)
                     /locus_tag="VFD08_00635"
     CDS             complement(6285..>10285)
                     /locus_tag="VFD08_00635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HZK09548.1"
                     /translation="PGLFLGLEKELAEKGHPQPEDLSFRLEDNNLYLLSEDYSWLKDH
                     LNDFILFFSIWKNDLHILLEEPKKRTSSSPVSDLEEKDEFHDSIDEELRRLVKQSAQI
                     KEVKKVRRPHKYLQEVKGPFLKISDILSEEQGGRVAIEGELLFLEKRELKNGKKLVDL
                     TVYDGSASLGCKLFCNDKDEEVLEKLLKIGEHYRFQGSTNYDQFSREIVLFPTRINLA
                     EKPAPKMDQAPLKRVELHLHSAMSEVDGVEDIEEYIKRAHKWGHKALAVTDLATAHAF
                     PKAYECIRKYDVKMLYGMEILYYDDEKKVVDIEDDVSFDGTFVALDLETTGLRNDRDE
                     IIEIGAVKIQNYNIVDSFHRLIKPKQEIPVFITELTNITNEMVSSAPELSEVFAELLD
                     FIGQDPLVAHNLDFDQGFLEKAATSLGYSFNPQGIDTLSLSRLVLRDIKRHRLNQVAK
                     RLKITQEAHHRALDDALVCGKIFISLLRLLENQGIEEFHEINALADKEYYISSSRKYT
                     MSLLCKNTDSLKKLYEMASISNLNYLQYSQPLVPATLLKEFRDYFYIGSGGSDGWLVD
                     NIIMCKPDEEILAFLDIFDYLEIQPPGQYARYLQRHVLKCLDDAREIHHKILQLGDVS
                     SLPVVATGDVRYLDKEDYLYRNIVRMGQNKNLSLEEEGNHYFRTTAEMLEEFTHLGER
                     AQEVVITNSLKIADSIEEIAPLPEGTFPPHIEGSEEELRNKCYQRAEKRYGSPLPELI
                     KDRLEYELGAIINNGFAVLYIIAEKLVNKSLDAGYLVGSRGSVGSSFAATMAGITEVN
                     PLPPHYYCSQCQYSEFVDESLYDNGFDMLDKKCPLCNTLLNKDGFNIPFESFMGFEGN
                     KEPDIDLNFAPTIQGTIHKYTEELFGKGHVFKAGTVSKVAERTAFGYIMKYYEGKEEE
                     IYSREMNRIIGRITGIRRSSGQHPGGIIVVPEDESIYNFTPIQYPANDPNSGVITTHF
                     DYDALEGKLLKLDILGHDVPSIIHDLENMTGVDSSIVRFDDQKILGIFNSTENLNLID
                     KSYDLKKGTLGIPEFGTNFVRSMLEDTKPEKLADLIRISGLSHGTNVWINNAQELIRS
                     GTITINDVIATREQIMLYGIQMGIPRDKAFNIMERVRKGKGLSEENLEVMKKANVPSW
                     YLNSCNRISYMFPKAHAVAYVLMSFRIAHYKVYHPLAFYASYFSTKVEDFDAENMTKG
                     IERVLFFLSEEKREKDMTKKEQDYLALMEVVYEMYARGLEFKPVDLYHSHAWKFLISE
                     NKILPPLRSISGVGLAAAEGIVKEREEEFISIEDLQTRAKLNRTAVEALRNHGCLEDL
                     KESNQISLFEFM"
BASE COUNT         2856 a         2170 c         1727 g         3532 t
ORIGIN      
        1 gcttctattt cttttaaact atagcagtct tttagtcctg cccaatcatt atgctcacgt
       61 acaagccctg acattatggc tcccgtacct aagttctgac ctaaatcatc tattaaagtg
      121 cgtatatagg tgccacgaga gcaatgaata cgaatacttc ccttttttcc atcaaaattt
      181 ataagttcaa gtttattaat attaatcttt ctttttttac gggggatttc tattccttgt
      241 cgagcatagt ggtaaagagc ctgtcctttg tatttaatgg cagagtacat aggtggaatt
      301 tgaaaagatt caccgataaa tttaccaagc tcctctttta attccctttc tgttggcgaa
      361 acaaagggct tgcttgtaat attaccccag atatctaagg tgtctgatgt ttgccctaaa
      421 ataaagtctg cctggtatac ttttacctct tcaggtaaaa gatcaataaa ttttgtagcc
      481 tgagaaacaa aacaattaag taccccagtg gccataggat caagcgttcc agagtggcca
      541 atttttttga ttccaagaag tcttcgaagg cgtgttactg cttgttgaga actcatgtcg
      601 gcttctttat ataagttgat cttgccccac atattcaagc tccttcaaca tcctttttaa
      661 agaaggtata tcttcttcag aaatagtcgc tccagcggct tttaaatgac caccaccacc
      721 aaaaaactgg gccaagtcat caacgttttg cacacctttg cttcgcaagg atagtttttg
      781 gctatcctcg taatcataaa ctaaggcggc tacatcaaca ttttctatat cccgaatcat
      841 agttataaca tcatctgttt caaaaaagcc agctttttgc atatcttctt ttttaagacg
      901 acaataaact atatttgggg cgataaattc aattttctct gtagcaagac ttaaaagcct
      961 aagaaggctt agtggttttt ctccaaagat ataacgatga attttttcaa tatctgcccc
     1021 tgcttcaagg agcttagctg cccattttaa agtgctgggg gtagttgagt tataaaaaaa
     1081 acgatttgta tctgttacta tagcgatata aatcgcatca gcgattttat ttgttaaagg
     1141 aatttctagt tcttccatca aaagtccaat aagttcccct gtactagaaa gtccaccata
     1201 aaccaagttt acatcaccgt agttagaatt ggtcgcatgg tggtcaatat tcaataaggg
     1261 aaggtgattg agatggttgt aacgctcccc caaacggctt aaatctgccg tatcaacact
     1321 gataagggca tcaaaatctc cctctggttt ggcagtatta tagcttcctt gaaaaaaagg
     1381 ctttagactg tgttgaagcg ggtcttcatt ccagattaca gccttttttc ccaaggattc
     1441 taatattagc ttaactgcca gtgcactacc caaggcatca ccatcggctt tgatatgggg
     1501 tagtacaatg aaataatggt atttttcgat aaaaacctta gccttattca agttcatcat
     1561 cgctataagt tattcctcga agcagctcat ccatctccat tcctttttcc acagaattat
     1621 catagaaaaa ttgtggttct ggtgtatggt aggttgttaa ttgtttgcct aatagcttcc
     1681 gaaaatatcc tgaagcatta tttaaaacca agagcacttc ttcacaatct tcttccccta
     1741 cccaacttac aaatatcttg gtttgcttca tatccgctgt tgtttcagcg ccaaggacat
     1801 ttatcatagt tggaagcctg ggatctttta cttcgaatat aagagaagtg cttataatac
     1861 ggcgtatttc ttcatttact ttacgcagtc tttgcttgtt catgaatcta agctcctttg
     1921 aatttccttg attatgaacg tttcaataat gtcgccctct ttaatatcat tgtagttttt
     1981 gattccaata ccacattcat agcctgtttg tacttggcgt acatcatctt taaagcgttt
     2041 aagagaagat acttctcctt catgaataac aataccatct cgaatgagtc gtaccataga
     2101 agttctagaa attttacccc tcataacaaa accccctgct attgtgccag caccaggaac
     2161 tttaaaggtg tcacgaactt cgatattgcc ttcaaccact tcttttcgat caggatcaag
     2221 cataccacta agtgcatctc gtacatcgtt tataatgtca tagatgaccc tataagtacg
     2281 tatttccaca ctatcacgtt cagcaatatt ttgaatatta ttatctggtc gaacattaaa
     2341 accgagtata acagcctcgc ttgctgtagc taaaagaata tctgattcag taatagctcc
     2401 aacctgcgcc ttaataatat tgaccataac ttcatctgta tttatttttt ccagggattg
     2461 cttgatggct tctacagatc cgtgcacatc tgctcttaaa ataatattaa gaacctttgt
     2521 ttctccatct tcgccttgca ctgtatctaa actagcaacc attttgggct gatggatacg
     2581 ctcagaacgc tctttatcag cattcttact tgcataaatc ctagccgttt gatcatcagc
     2641 agttacatag attttctctc ctgccatggg tacttcatta agaccagtga ttttaaaagc
     2701 agaagaaggc ccagcaattt tgaggcgttt tcctctgtca tcaatcatgg ttctaacgcg
     2761 tccataactt gagccagcaa atacattgtc tcctatttgg attgttcctt tttcaagcag
     2821 aactgtagcg gtcgaaccca ttcgtcgatc aacattggct tcaataattg ttccaacgcc
     2881 aggacgctct gggttggcct ttaattctag catttctgct acaagaagaa ccatttccaa
     2941 gagggtttct attccctcgc cagttatggc tgaaacttct acagcaataa cgtccccacc
     3001 ccagtcttct acaagaagat tgtgttcggt taaatcctgt ttaactcgct caggatctgc
     3061 tgcgggtttg tccattttat taatggcaac tataatcgga acaccagctg acttggcatg
     3121 atggattgct tcaacggttt gtggtttaac accatcatca gctgctacaa caaggattgc
     3181 tatatcggta attaaagctc ccctggctct tagcgttgta aaggcttcat gtcctggtgt
     3241 gtcaagaaat acaatttttt tgccttttga atgaatctca ctggctccaa tgtgctgagt
     3301 aattccacca gcttctcttt ttgtaactct agtttgtcta atagtatcta aaagtgaagt
     3361 tttaccatga tcgacatggc ccataacggt aactaccgga gcgcgttctt ctaaatcatc
     3421 cttactatct tcataatcaa atctttcttc ttcgatttca gatagggatt gactacgtgt
     3481 aataactagt tcatattcta gggctagtag ttcagcatct tcaaaggaga tattagagtt
     3541 aattgtatac atctctccca tgtccataaa cttcattatt agctctgtag caggtttttg
     3601 aattttttca gcaaattcac ctagagtaat atggtcatct atttctattt cagaagaaga
     3661 tagatcaacc gttttgtctt ctttttcttt acggtattga gctcttgttc ctctttttct
     3721 tttttgccaa tttctttttg gtgttgctgg tgtctctgtt tctttctttg aaggttctgc
     3781 tgttttttta ctggattctt tttctttctt tgacgagggc tttttttcgt cagttttttt
     3841 ctcagccttg tctctttttt cttttgcagg ctcttttatt tctttgctac tttcttctgt
     3901 tggcaaatca ccgcgaacaa agtcgataaa tttatcgtgc tcttcttttg taagtgtgct
     3961 catgtgattt gatactgtaa taccaatgtt ttctaatttt acaataaggt ttttactagt
     4021 cattttatat tcttttgcta gttcataaat tcgtattttt ctcaaagaat cacctcctgg
     4081 atatctcttt gatcatattc tctgccagct ttcctttttt tactgcaaaa acatggcggt
     4141 tttttacccc agtggtttta gacattcgct ctgaaggacc aagtataata tggtcaatat
     4201 cagaatgata aaggaaggag gctattgttt tgttactaca ttcttctgag aaaagaagga
     4261 gcccaaggct atcttctttt aaagctcttt ttattgcaaa ggttccggaa acaagcactc
     4321 cggattttgc ggcaaaacct aatagtgtat aagttttatt ttcccaattt ttgtcggagt
     4381 tcatctattg tctcctgttt tatcatagat ttaaaactgc gctgcaagcc tttttctttc
     4441 aaagcttgtt caaaacaggc cttatccttg cacagatagg ctccacgtcc attgagtttt
     4501 cctgtgggat caattgaaac gtgtccctct ttagagcgta caaggcgtat aagttcacgc
     4561 ttgtctttta gttgcccaca tccaacacat tttctaaggg gagttttacg ctttttcata
     4621 atttttactt tcgagatttt gactttcgct aaggatatct attctaatgc cagtaagttt
     4681 agccgctagt ctagcatttt ggccttcttt accaatagca agggaaagtt gaaaatccgg
     4741 tactataacc ttggcactgc gttcttcaag aactcttacc tcggagactt tggccgggct
     4801 aagggagttt tcgatgaatt taggtaaatc atctgtatat tcaacaatat caatcttttc
     4861 tcctcctagc tcatctacaa tatgggcaac cctgcttcct tttggtccaa cacaagctcc
     4921 aacaggatct agaccttccc taccagcctg tacagcaatc ttggttctgc ttccgggttc
     4981 tctggcaatt gacttgatta agacatcccc ttcgagaatt tctggaattt ccatttcaaa
     5041 aagtcgccgc acaagtcctg ggtgggtacg agagaggata atttcaagac cttttttacc
     5101 tctaacatcc ctggggtttt ttcctgcttc aagacttaat cgtttttcgt cttttacttc
     5161 aataatataa ctacgtaaac gatcacccac tcgataacgc tctcctggta tttgttcttt
     5221 ggcaggcatc attgcttctg cctttccaag attaaagtaa acaacaccat tattgatacg
     5281 ttggatttga gagtagacca gttcctgttc tctatttttg tactcatcat atacactggt
     5341 gcgttctgct tctcgtatac gttgaactac gacctgctta gctgctagag cactcactct
     5401 accaaaatcc ttgggtgttt cttctttttc tagtacatca tcaagttcat aaactggatt
     5461 aatcttttta gcctcttcta ggctaatttc aagctcagca tcctcaacct cttctaccac
     5521 attgtaacga gaaaatactt ttatatctcc actgtcacgg ttgatatcaa ctcttgcctg
     5581 acgatttttt ccaaagtttt tacgataagc tgtaactagg gctgcctcaa ttgactcaaa
     5641 tagagtttct tcaggaatat ccctttctct agctatcata tgaattacat ctgtaatctt
     5701 atccattatt tacctcaaaa ttcgatattt gttcttgctt tagcaaccag atctacagga
     5761 acttctagct cttttcctgt aatatcatca agtattttta tatatttatt atctttagaa
     5821 aggagtttcc catttatcac tttttgatct tgaaatttag aaaaaagact gagttgaata
     5881 gaatggccaa taaaccttct atagtgatca tcttttttaa gggttctttc tataccgggc
     5941 gaactcactt ccaagaaata ttgtttttta aatggatctt ttttatccaa agctttactt
     6001 aaaaactgac ttacttcttc gcactcattt aaactaatgc ctttttcact atcaataaaa
     6061 acacgcagat aatgatgtgg tccttctttt acaaaggcaa ggtcataaag ttctagatcc
     6121 ttagattcca aaaagggtgt caatatttcc tctgctactt tactaatact tttcacctta
     6181 acctcctttt tccattatta agaacgaaag agtgggttgc ccactctttc gaaacgcttc
     6241 atcttatgaa ttatagcata aaaagcgcta attttcaaga cattttacat aaactcaaac
     6301 aagctaattt gattgctttc cttaaggtct tctagacaac catggttacg aagagcttca
     6361 acagctgttc gatttagttt tgctctagtt tgaagatcct caatgctaat aaactcttct
     6421 tctctttctt tgacgattcc ttcggctgct gctagaccta ctccacttat ggaacgaagg
     6481 ggtggaagga ttttgttttc agaaataagg aatttccaag catgagagtg gtaaagatca
     6541 acaggcttaa attcgagacc tcttgcatac atttcatata caacttccat taaagccaaa
     6601 taatcctgct cttttttcgt catgtctttt tctctttttt cttcgcttaa aaagaaaaga
     6661 actctttcta tccccttggt catattctct gcatcaaagt cttctacctt ggtactaaag
     6721 taagaggcat aaaaagccaa gggatgatag accttataat gggcaattct aaagctcatt
     6781 agtacatagg caacagcatg agctttagga aacatataag aaattcggtt acatgaatta
     6841 agataccaag agggaacatt ggcctttttc ataacctcta agttttcttc actcaggcct
     6901 tttccttttc taactctttc cattatgtta aaggccttat ccctagggat ccccatttgg
     6961 atcccgtaga gcatgatttg ttcacgggtt gcaataacat catttatagt aattgtgcca
     7021 cttcttataa gctcctgggc attattaatc caaacatttg taccatgact taatcctgaa
     7081 atacgaataa gatcggcaag tttttcaggt ttagtatctt caagcatgct tcgaacaaag
     7141 tttgtcccaa actctggtat acccaaggtt ccttttttta agtcatagct cttgtctata
     7201 aggtttaaat tctcagtaga attaaatata cctagaattt tctgatcatc aaagcgaaca
     7261 atggagctgt caaccccggt catattctca agatcatgga tgatacttgg aacatcatgg
     7321 ccaagaatat ccagctttaa tagtttgcct tctagcgcat cgtagtcaaa gtgagtcgta
     7381 ataaccccac tattagggtc attagcaggg tattgtatgg gagtaaaatt atagatactc
     7441 tcatcttcag gaacaacaat aattcctcct ggatgctgac cactacttct tcggatccca
     7501 gtgattcttc caatgatacg gttcatttct cgggagtaaa tttcttcttc ttttccttcg
     7561 taatatttca taatgtagcc aaaggccgta cgctcagcaa ccttactaac tgtgcctgcc
     7621 ttaaagacgt ggcctttgcc aaaaagctcc tcggtatact tatgaattgt tccttgaatg
     7681 gttggagcaa agtttaagtc aatatcaggc tctttatttc cttcaaaacc cataaaggac
     7741 tcaaagggga tattaaaacc gtctttattt aaaagggtat tacacagggg gcatttctta
     7801 tctagcatat caaaaccgtt atcataaagt gattcatcga caaattcgct atactgacac
     7861 tggctacagt agtaatgagg aggaagagga ttgacttcag taatgcctgc catagttgca
     7921 gcaaaggaag aacctaccga tccacgactt ccaacaagat aacctgcatc cagagatttg
     7981 tttactaatt tttcagcgat aatatacaat acagcaaagc cattatttat aatagctcct
     8041 agttcatatt ccaggcggtc ctttataagt tctggcagag gactgccata tcttttttcc
     8101 gctctttgat agcatttgtt tcgaagttct tcttcactcc cttcaatgtg aggaggaaaa
     8161 gtaccttcag gcaagggtgc tatttcttca atactatctg ctatttttag actgtttgtt
     8221 ataactactt cttgcgctct ttctcctaaa tgtgtaaact cttctagcat ttcagctgtg
     8281 gttctaaaat agtgattgcc ctcttcttca agggagaggt ttttgttctg ccccattctc
     8341 actatatttc tatatagata atcctcctta tctagatagc gtacatcacc tgtagctacc
     8401 acaggaagcg aggatacatc acctagttgt aagattttgt ggtgaatttc tctggcatca
     8461 tctaggcatt ttaagacatg tctttgcaaa tacctggcat actgccccgg aggttgaatt
     8521 tcaagataat caaagatatc taaaaaggca agaatctcct catctggctt gcacataata
     8581 atattatcta ctaaccagcc atcacttcct ccactaccta tgtaaaagta atcacgaaac
     8641 tcctttaaga gagtagcagg aactaggggt tgactgtatt ggaggtaatt aagattggaa
     8701 atactagcca tttcatataa cttcttaaga gagtctgtgt ttttacagag caagctcatg
     8761 gtgtattttc gtgatgaaga tatataatat tctttatctg caagtgcatt aatctcatga
     8821 aattcttcta tgccttgatt ttcaagaagc cgcaaaagtg atataaagat tttaccgcat
     8881 actagggcat catctagtgc cctgtggtgt gcttcttggg tgattttcag gcgcttagca
     8941 acttgattga gacggtggcg cttaatatca cgaagaacca gacgactaag agatagtgtg
     9001 tcaatcccct ggggattgaa actatagccc agtgatgttg ctgccttttc taaaaaacct
     9061 tgatcaaaat ccaaattatg agcaacaagg ggatcttgac caataaaatc taaaagttca
     9121 gcaaaaactt ctgataactc aggagctgag cttaccattt cattggttat atttgttagt
     9181 tcagttataa agacaggaat ttcctgctta ggtttaataa ggcggtgaaa actatctact
     9241 atattgtagt tttgtatttt aactgcacct atttcaatga tttcatctcg atcattgcga
     9301 aggccagtcg tttctaagtc aagtgcgaca aaggtaccat caaaggaaac atcatcttct
     9361 atatcaacaa ctttcttctc gtcatcataa tagaggatct ccatcccata tagcatctta
     9421 acatcatatt tccttatgca ttcataggct ttaggaaagg cgtgggccgt tgcaagatca
     9481 gtaaccgcta aggctttatg tccccactta tgggctcttt ttatgtattc ttcaatatcc
     9541 tcgaccccat caacttcact catagcactg tgaagatgaa gttctactct tttaagaggg
     9601 gcttgatcca ttttaggggc tggtttttca gctaaattaa tgcgagttgg aaaaagcaca
     9661 atctctcgac taaactgatc atagtttgtg cttccttgga agcggtagtg ctcccctatt
     9721 ttaaggagtt tttctaatac ttcttcatcc ttatcattac aaaataactt acaacctaaa
     9781 gaggcactgc cgtcataaac agtcaaatca accaattttt ttccattttt caattctctt
     9841 ttttcaagaa aaagaagctc accttcaata gctacccttc cgccttgctc ttctgataaa
     9901 atatcactaa tttttagaaa ggggccctta acctcttgaa gatatttatg aggccgcctt
     9961 acctttttca cttccttaat ctgagcactt tgtttaacta aacgacgcaa ttcttcgtca
    10021 atagagtcgt ggaattcatc cttctcttct aagtcactaa ctggtgagct agatgttctt
    10081 ttcttaggct cttctaataa gatatggaga tcatttttcc aaatagagaa aaataagatg
    10141 aaatcattta aatggtcctt tagccaagaa taatcttccg ataacaaata cagattattg
    10201 tcttctaatc gaaaggatag gtcttcaggt tgaggatgac ctttttcagc cagttctttt
    10261 tctagaccta aaaatagccc tggta
//
LOCUS       DASRNA010000022         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_754513,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000022 DASRNA010000000
VERSION     DASRNA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754513"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..957)
                     /locus_tag="VFD08_00640"
     CDS             complement(187..957)
                     /locus_tag="VFD08_00640"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HZK09549.1"
                     /translation="MIDIHNHILPGIDDGSRDMAMSVEMAKVFLDQGVKKIIATPHYI
                     QGVTTNSFSENKIILADFRGRLKEENIPLEVYLGHEIMISPDIIRTLEEKHIASLNNT
                     SYVLVELPMLDMPIYTDSILYELLVRGYQPIIAHPERYAPVVEDPSVLFELVEKGALA
                     QLNLPSLEGKYGKKIAKTAQILLEHNLIHFVSSDAHSSSSRSPHIKNSLEILKKLVSK
                     EDFYLLTHKNAQDLLEDKKIQTKIPLKYKKPKFFFFNR"
     gene            complement(963..1697)
                     /locus_tag="VFD08_00645"
     CDS             complement(963..1697)
                     /locus_tag="VFD08_00645"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HZK09550.1"
                     /translation="MSKHLNTLITLRNPKSPISEAYRTLRTNIQFSNVDKELKTIIVT
                     SAVPGEGKSTTVSNLAIAMAQAGKKVVLIDCDFRKPILHKRFFLSNIEGLTNVLVGEK
                     GIYEVIQFPEGIENLSVITSGPIPPNPAELLDSNKMQTVLKELKDKVDIILMDTPPVG
                     VVTDAAILSTKVDGTLLAIAHKKTEIEMVQTAVGLLDKVGANIIGTVLTQIDMTDKSA
                     YQYGNYEYYGETPLPKKTKRRRPRRG"
     gene            complement(1711..2406)
                     /locus_tag="VFD08_00650"
     CDS             complement(1711..2406)
                     /locus_tag="VFD08_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZK09551.1"
                     /translation="MPTNMEYSEEISLKELFYILRKRFVLILALTILAMTIAGLVSFY
                     VLEAEYQTSTTLMVGKPKDYQGESGIEYNELMLNQKLVSTYGEMVKMRVVADEVIKDL
                     GLEMSYEQFKSKVNVNLVKNTEIIELSVSDNDPLLAAKIANHTAQVFIRTVKDIMLVE
                     NVQVIDTAIVPNNPIKPKKLMNIAIAGVLGLMLGVFISFLMEFLDNTIKSKEDIERHL
                     GLPVLGMIPDDRH"
BASE COUNT          649 a          466 c          457 g          865 t
ORIGIN      
        1 agtgggctgg gacttaccat tgctcaggag attgctcttc gccacaagat gaaaattgag
       61 atagaaagct ctcctggaaa taccttggta tgctttgttt atcagctaga ttaagcctac
      121 ttgtggccca agtaaaaaaa gacatctaga tgtctttttt tactgtaata tataggcaag
      181 cttcttttat cgattaaaaa agaaaaactt aggtttttta tatttaaggg gaatcttggt
      241 ctggattttt ttgtcttcta atagatcctg ggcatttttg tgggttaata ggtaaaaatc
      301 ttccttagaa actagttttt ttaggatctc aagggagttt tttatatggg gagacctgct
      361 actactacta tgggcatcag aagaaacaaa atggattaga ttgtgttcta agaggatctg
      421 cgctgtttta gctatttttt tgccatattt tccttctagg ctgggaaggt ttaattgggc
      481 tagggcgccc ttttctacta gttcaaagag gacactagga tcttctacca caggagcgta
      541 tctttcggga tgggctataa tgggttgata tccccttact aggagctcgt ataggatact
      601 atctgtatag ataggcatat caagcatggg tagctcgact aaaacatagc tagtgttgtt
      661 taagctggca atatgttttt cttctagggt ccttattata tcgggtgaaa tcataatttc
      721 atgacccaaa tagacttcta gagggatatt ttcctctttt aatctgcccc taaaatctgc
      781 tagaattatt ttgttttctg aaaaagagtt tgttgtcact ccttggatat aatggggagt
      841 ggcaataatt tttttaaccc cctgatcaag aaatacttta gccatctcta cagacatggc
      901 catatcccta gatccatcat caatgccggg taatatatgg ttgtgaatat ctatcattca
      961 catcaacccc ttctaggccg gcgtcttttg gttttttttg gtaggggtgt ttctccataa
     1021 tactcataat ttccatactg ataggctgac ttatctgtca tatctatctg ggttaaaaca
     1081 gtaccaatta tatttgcgcc taccttatct agtagtccca cggccgtctg gaccatttct
     1141 atttcggttt ttttatgggc gattgccagc agggtcccat ctacttttgt agaaagaata
     1201 gcggcatcgg taacaacccc cacaggggga gtatccatta gaataatatc caccttgtcc
     1261 tttagttcct ttaagaccgt ttgcatttta ttggagtcta aaagttcagc gggatttggt
     1321 gggatgggac ctgaggtaat aacgctgagg ttttctatgc cctcaggaaa ctgtataact
     1381 tcataaatgc ctttttctcc caccaggaca tttgttaggc cttctatatt gcttaaaaag
     1441 aatcttttat gtaaaatggg ttttctaaaa tcacagtcaa ttaaaaccac ctttttgccc
     1501 gcctgagcca tggcaatggc aagattagac acagtggtgc tttttccctc tccaggcaca
     1561 gctgaagtaa ctataatggt ctttagctct ttatctacat tggaaaactg tatatttgtt
     1621 cttagggtcc tataggcttc ggatatagga gattttggat ttcttaaggt tattaaagta
     1681 tttaggtgtt tactcattta aactctcctg ttaatgtcga tcatcaggaa tcattccaag
     1741 aaccggcagt cccaaatgtc tttctatatc ttcttttgat ttgatggtat tgtctaaaaa
     1801 ttccattaga aagctaataa atactcccag cataagacca agcactccag cgatggcaat
     1861 attcataagt ttttttggct taatggggtt atttggaaca atggctgtgt ctatgacctg
     1921 gacattttca acaagcataa tgtcctttac tgttcttata aagacttggg ccgtatgatt
     1981 ggcaatttta gctgctaata aggggtcgtt atcgctaacg cttaattcga taatttccgt
     2041 gttttttact agatttacat ttaccttgct tttaaactgc tcatagctca tctctaagcc
     2101 aagatctttt atcacttcat ctgctaccac cctcatcttt accatctccc cgtaggttga
     2161 tacgagtttt tggtttagca taagttcatt atactctatc ccgctttcgc cttgataatc
     2221 cttgggttta ccaaccataa gagtggttga agtttgatat tctgcttcta atacataaaa
     2281 acttaccagt ccagctatgg tcatagccag gatggttaaa gctaggatta gaacaaatct
     2341 ttttcttaag atataaaaca actcttttaa acttatttct tcgctatatt ccatatttgt
     2401 tggcattttt tctctccact atttttttct tcaatcc
//
LOCUS       DASRNA010000023         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_903976,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000023 DASRNA010000000
VERSION     DASRNA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903976"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1046)
                     /locus_tag="VFD08_00655"
     CDS             complement(192..1046)
                     /locus_tag="VFD08_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZK09552.1"
                     /translation="MFNFRLKTFLALCETRSYTRAAQRLHITQPSVTQHIQYLENYYN
                     CDLFYYKGRELSLTPQAIILKKHANSLRYQEKKLLESLKPSAGYSLKIGATKTIGEYV
                     IPGQLSRFLASPQNKLSVEIDNTRRILTLLDKGQIDFGIIEGFFDKLTYESKLYGREA
                     FVGFCSKNHPFAGRKISIEDLLQEDLIVREKGSGTRKILEQLLLNHNYSFESFSRTIC
                     INNFGLLESLVANDCGVTFAYLAAGINNSALDSFAVKGWSLSRDFNYVYLKDTQAKKL
                     VEVFNQYK"
     gene            1153..2190
                     /locus_tag="VFD08_00660"
     CDS             1153..2190
                     /locus_tag="VFD08_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZK09553.1"
                     /translation="MEKTKKYLEILPGFGAALLIAIVAVGLEGLLPIHLIGASVIALF
                     IGLIINHFYQPSEILVSGLHFTSKRILKFAIILLGASLSIGTILEVGKMSLTVMLFTL
                     LTCFGGGFFVGKALGLNWKLSNLISAGTGICGGSAIAAVAPVIDAKDSDIAYALSATF
                     LFDMAMIILFPIMGRALGLSDMAYGLWTGTAVNDTSSVVAAGYAFSEAAGDFAAMVKL
                     TRTLSIIPVVLIFALVQVRIKTKEAKMDADGAKISANIEFVKLFPWFILGFIALALAN
                     SFGFIPEKVAINSKAISKFLMVVALASIGLNTSFKEMKQAGAAPLIHGFIISAAVVVV
                     AIGVASLMGIV"
     gene            complement(2317..2712)
                     /locus_tag="VFD08_00665"
     CDS             complement(2317..2712)
                     /locus_tag="VFD08_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HZK09554.1"
                     /translation="MKKISFILLVLILLTSCSAPLSEDFDEKMIEDKVGLILDYLNNK
                     QTDKLLELSTVTLKDGLTEETMSTVYGLLEEAGKFEEIISISNGSTTDKNTKEEFALS
                     IIKTKYELKEITYTMTFTKQYKLAGLFMK"
     gene            complement(2699..3295)
                     /locus_tag="VFD08_00670"
     CDS             complement(2699..3295)
                     /locus_tag="VFD08_00670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HZK09555.1"
                     /translation="MKALTYFILGALCFALTQIFTRIPLLNLLMDKPSFIIFFHQHFI
                     FAGFLLALSAGVFEEGGRFLFKKYLLKPIKSTALEPFLFGLGHGLCEAVYLLGPLLSV
                     YPISLLLPALFERVIALVLHICFSFIVWNGFQKNKPFKYLLLAVLAHTLVNFSIPLGQ
                     TADSTTLLWFLILLQTVLLFIYSLYSLKFYRRTFHEKD"
     gene            3462..4019
                     /locus_tag="VFD08_00675"
     CDS             3462..4019
                     /locus_tag="VFD08_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HZK09556.1"
                     /translation="MKKTWKDVLDFWFDPKNMPLHFEEDDSFDQKIREEFLETWNRAS
                     QGLLMDWRDSLKGRLAEIIVLDQFSRNLFRNDRRSYSQDKMAIALSQEALKDPGYEKL
                     SPDEKRYILLPFMHSESLELHDWVRPFFEKMGDENTLYFEKLHYHVLKRFGRYPYQNK
                     DLARDSTVGELEYLEEKAGKHYSKT"
     gene            4097..5287
                     /locus_tag="VFD08_00680"
     CDS             4097..5287
                     /locus_tag="VFD08_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012863704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK09557.1"
                     /translation="MKEKLKNNVYWVGKIDWELTKFHGDDYSITSGSSQNAYLIEEEK
                     TVLIDTVWTPHKDEFIENLEKEIDLAKLDAVIIQHGEVDHSGGLVKLMEKRPDLPIYC
                     TANGVKSLVGQFHHPEWDYRVVKTGDSLNVGPGKDLVFVEMAMLHWPDSMASYLTGDN
                     ILFSNDAFGQHFAVAELFNDKADQCLLYKEAIKYYANILTPFQAIAKKKIDEILAMNL
                     PIEIIAPSHGVIWRDNPLQIVEKYIEWSDQYKEDQITIAYDSMWDGTKKLAHALEKHL
                     RDLAPDTVVKVFNVALADKNEVMTEVFKSKAIAVGSPTVSNDILSSVGGWLYFLKELK
                     FKGKKAAAFGCYGWSGEGNKKIKEQLEGAGFSVLEPDVRVNWAPREENLDQMSEIAQA
                     LIAD"
     gene            5574..6173
                     /locus_tag="VFD08_00685"
     CDS             5574..6173
                     /locus_tag="VFD08_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK09558.1"
                     /translation="MGEEKTAKSDDAMPIIDMRLRILRAATSLMTEKGIKETTLKDIA
                     QKAGISKGTLYYYYSAKEDIIYDIANKNLKQVTDGLMVMLDDADSNVSAEEIIKSLFE
                     NILDAQTRGKLHIYLLNNIATTNNALAEKFEMCYNDWRKTLERGLDKVMPASKGDHVA
                     LSHLILATIDGLIIQKMCGVEDIPVDEIVKLLIKTNTNS"
BASE COUNT         2043 a         1081 c         1321 g         1802 t
ORIGIN      
        1 agttagtaaa agatctacta gcccgaactt tgtttttttt cttagggaga ttttcttttt
       61 ttattagagt atatccatct agaaagacgc aaagaaagcg cagccccatc cacacaatca
      121 aaccccttgc ttatagctaa agatagggtg accatcttca tctgctattt tgataaaaca
      181 ccagggccct actatttata ctgattaaat acttctacca gcttcttggc ctgtgtatct
      241 tttaagtaga cataattaaa gtctcgacta agagaccaac ccttaacagc aaaagaatct
      301 agcgctgaat tattaatacc agcggcaaga taggcaaagg ttacaccaca atcattggca
      361 acaagagatt ctagcaggcc aaagttattg atgcaaatcg tccggctaaa actctcaaag
      421 gagtaattgt gatttaataa tagttgttca agaatttttc gtgtcccaga tcctttttcc
      481 cggacaatca gatcttcctg gagtagatct tctatagaaa tcttgcggcc ggcaaaggga
      541 tggtttttgc tacaaaaacc tacaaaggct tccctgccat atagcttgct ttcataggtc
      601 aatttgtcaa aaaaaccctc gattattcca aaatcaattt gtcccttatc taagagcgtg
      661 agaattcgtc tagtattatc aatttctacc gaaagtttgt tctgtggtga ggccaaaaaa
      721 cggcttagct ggcctggaat tacatactca ccaatggttt tagtggcccc aattttcaaa
      781 gaatagccag cagaaggttt tagtgattca agtagctttt tttcctggta acgtaaagaa
      841 ttggcatgct tttttaggat aatagcctgg ggcgttagcg ataattcacg gcccttgtag
      901 taaaacagat cacaattgta gtagttttcc aaatactgaa tatgctgggt tactgagggc
      961 tgtgttatat gtaagcgctg ggcagccctc gtgtaacttc ttgtctcgca tagggctaaa
     1021 aaagttttaa gtctaaaatt aaacaagaca gacctccata agaaaatatt atagactgat
     1081 agataattat aattagacct tatcactact tttctatata atcaatagat agtctagaaa
     1141 gaggtgggtt agatggaaaa aacaaaaaag tatctagaaa tccttccagg ctttggtgca
     1201 gctctactta ttgctatagt agcagtgggc ctagagggtc tactaccaat tcacttaata
     1261 ggagcttctg ttattgctct atttattggc cttataatca atcattttta tcagccttca
     1321 gaaatccttg tcagtggact tcattttacc tccaaaagga tattgaagtt tgccattatt
     1381 ttactgggag cctccctaag tattggaaca atccttgaag taggtaaaat gtccttaacc
     1441 gttatgctct ttaccctgct tacttgtttt ggtggtggtt tttttgtagg aaaagccctt
     1501 ggcttaaact ggaagttatc caatttaatt tcagctggca caggaatttg tggcggttct
     1561 gctatagcag cagtggctcc ggtaatcgat gccaaggata gtgatatcgc ctacgcccta
     1621 tcggctacat ttttatttga tatggcaatg attattttat ttcctattat gggacgggct
     1681 cttggcctta gcgatatggc ctacggccta tggacaggaa cagcggtaaa tgatacttct
     1741 agtgtagttg cagcgggcta cgctttttca gaggctgcgg gtgattttgc tgcaatggta
     1801 aagctaacaa gaactctttc tattattccg gtagtattaa ttttcgcttt ggtgcaggta
     1861 agaataaaaa caaaggaagc aaaaatggat gcagatggag ctaaaataag tgcgaatatc
     1921 gagtttgtaa aactatttcc ctggtttatt ctaggcttta ttgcccttgc ccttgcaaac
     1981 agttttggat ttataccaga aaaagttgca ataaactcca aggcgattag taagtttcta
     2041 atggtagtgg cacttgcctc aataggccta aacaccagct ttaaagagat gaaacaagca
     2101 ggagctgcac cactaatcca cggttttatt atttctgcgg cagttgtggt ggtggctatt
     2161 ggggttgcct cccttatggg aattgtataa ggggctattt gccagctagg aagcagcact
     2221 gttttgaaaa tgacataaga aaaaagataa gcgatatttg gtaaaaatac caagcatcgc
     2281 ttatctttta ttgccctact tcctctggca gttttcttat ttcataaaaa gccccgcaag
     2341 tttatattgt tttgtaaagg tcattgtata ggtaatttct ttcagctcat atttggtctt
     2401 gattatgcta agcgcaaatt cttcttttgt atttttgtct gtagtagagc catttgaaat
     2461 ggaaatgatt tcctcgaact ttcccgcttc ttctaaaagt ccatagacag tagacattgt
     2521 ttcttcagtg agtccatcct ttagcgttac agtagatagc tcaaggagct tatccgtttg
     2581 cttattattt agataatcaa gaataaggcc taccttatct tctatcattt tttcgtcgaa
     2641 atcttctgaa agaggggcgc tacaggaagt aagtagaatg agaacaagaa ggataaaact
     2701 aatctttttc atggaaggtc ctcctataaa atttgagtga gtagagacta tagataaaaa
     2761 gcagcacagt ttgtaatagg atcaaaaacc aaagaagtgt ggtgctatct gctgtttgcc
     2821 ctagggggat agaaaaattg accagggtgt gagcgagcac ggcaaggagt agatacttga
     2881 agggcttatt tttttgaaag ccattccaga caataaagct aaagcaaata tgaagaacaa
     2941 gcgcaatcac tctttcaaaa agagcgggta aaagcaggga tatgggataa acagacaaaa
     3001 gtggtcctaa aagataaact gcttcacaga gcccgtggcc tagtccgaat aaaaagggct
     3061 ctagagcggt actttttatg ggctttaaca aatatttttt aaaaagaaag cgcccgcctt
     3121 cctcaaaaac tccagcagaa agggccagca aaaaacctgc aaaaataaag tgctgatgga
     3181 aaaatatgat aaaagaaggt ttatccatca ataggtttaa aagcgggatg cgagtaaaaa
     3241 tttgtgttag ggcaaaacag agggccccta aaataaaata ggtgagagcc ttcatcaaag
     3301 ctcctttttg taaaagggag taggatccat taattaaata gtacagtatt ttttataatc
     3361 tagcaatgat aagaaaaaaa aggggtataa gaaaaaacag gacatataca agaaggaccc
     3421 actaatccat aaaaggatgt ttgttataaa aggagcccag tatgaaaaaa acatggaaag
     3481 atgtattgga cttttggttt gaccctaaaa atatgcccct acactttgaa gaagatgata
     3541 gctttgatca aaagataagg gaggaatttt tagaaacttg gaatagagcc agccaaggac
     3601 ttttaatgga ttggcgcgat agccttaagg gcaggctggc agaaattatt gtcttagacc
     3661 aattttctag gaatttattt agaaatgata gaagaagcta tagccaggat aaaatggcta
     3721 ttgctctttc ccaggaagcc ctaaaagatc ctggctatga aaaactaagc cctgatgaaa
     3781 aacgctatat tcttttaccc tttatgcact cagagtcttt ggagcttcac gattgggtta
     3841 gacctttttt tgaaaagatg ggtgatgaga atactcttta ctttgaaaaa ctccactacc
     3901 atgtattgaa aagatttgga cgttacccct accaaaataa agatttggct agagactcaa
     3961 cagtaggaga attggaatat ctagaggaaa aagcgggaaa acactatagc aaaacttgat
     4021 ttaagtcaaa gaaaccccct ctaaataagg ctaagatgaa atcaagtgaa ttcattaaag
     4081 gaaaaaagga gtagaaatga aggaaaaact aaaaaacaat gtgtattggg tgggaaaaat
     4141 tgattgggaa ctaacaaagt ttcacggcga tgactattca atcacgagtg gttcctctca
     4201 aaatgcctat cttattgaag aagaaaaaac ggtacttatt gatacagtct ggactcctca
     4261 taaggacgag tttattgaaa accttgaaaa ggaaattgat ctagcaaaac tagatgccgt
     4321 gattattcaa catggtgagg tagatcactc tggagggctg gttaagctaa tggaaaaaag
     4381 acctgatctt cctatttatt gtactgccaa tggggttaag agtctggtag gtcagtttca
     4441 ccatcctgaa tgggattacc gggttgtgaa aacaggagac agtttaaatg taggcccagg
     4501 caaggatctt gtttttgtag agatggccat gcttcactgg ccagactcca tggcatcata
     4561 tcttacggga gataacatcc tctttagtaa tgatgccttt ggccaacact ttgccgtagc
     4621 tgagcttttt aatgacaagg cagatcaatg tcttctttac aaggaagcca ttaaatacta
     4681 tgcaaatatc cttacacctt ttcaagcaat tgctaagaaa aaaatagatg agattcttgc
     4741 aatgaatttg cctattgaaa tcattgctcc tagtcatggt gttatttgga gagataatcc
     4801 tctgcagatt gttgaaaagt atattgagtg gagcgaccag tataaagaag atcaaattac
     4861 cattgcctat gattccatgt gggatggtac aaaaaaactg gctcatgctt tggaaaaaca
     4921 cctaagagat ctagctcctg atactgtggt aaaggtcttt aatgttgccc tagcagacaa
     4981 aaacgaagta atgactgaag tttttaaatc aaaggccatt gcagtgggtt ctcctaccgt
     5041 tagtaatgat attctttcct cggtgggagg ctggctatat ttcctaaagg aattgaaatt
     5101 taaaggaaaa aaagcagcag cctttggttg ctatggttgg agtggagaag gcaataaaaa
     5161 gattaaagaa cagctggagg gggcaggatt ttctgtcctt gagccggatg taagagtaaa
     5221 ctgggcgcca agggaagaaa accttgatca gatgagcgaa attgcacaag cactcattgc
     5281 tgactaggct agtaagtaaa agaaggggta aatatatcta gtcctttaaa ggaagtagtc
     5341 acttggccta ctaggctttt agctaaatta tatgcgctta tcgtttctag acggtggcgc
     5401 atattttatt gcaggctagc cctatattta ggagtcattt gttcgtgtta aaaattgtac
     5461 aagccttgat aaaacaaagg tcctagcatt ttgatttctt gacaagagcc aagagattaa
     5521 gtacaattca agtatcgacc ggtcaactga ccgataaaga agcagggggt ataatgggtg
     5581 aagaaaagac agctaagagt gatgatgcaa tgccaataat tgatatgcga cttcgtattt
     5641 taagggcagc aacaagtttg atgacagaaa aaggaatcaa agaaaccact ttaaaagata
     5701 ttgcccaaaa agcaggtata agcaagggca cactatatta ctactactcg gccaaagagg
     5761 atattattta cgatattgcc aataagaact taaaacaagt taccgatggg ttaatggtta
     5821 tgctagatga tgctgatagc aatgtctcgg cggaagaaat catcaaaagc ttatttgaga
     5881 atatcctgga cgctcaaaca agaggcaaat tacatatcta tctccttaac aatatagcaa
     5941 caacaaataa tgccttggct gagaaatttg agatgtgcta caatgactgg cgcaaaacac
     6001 tggaaagagg cctagataag gttatgcccg ctagtaaggg ggaccatgtg gccctatctc
     6061 acctaatact agctacaatt gatggtttga ttattcaaaa aatgtgtggt gtagaggata
     6121 ttcccgtgga tgaaatcgta aagctcttaa tcaagaccaa taccaatagc taaatacagt
     6181 aagtggcaag tcttattttc attttataca tatgcaggat aggaataaat ttatctaagt
     6241 caaagga
//
LOCUS       DASRNA010000024         8188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_380508,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000024 DASRNA010000000
VERSION     DASRNA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380508"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFD08_00690"
     CDS             complement(<1..283)
                     /locus_tag="VFD08_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK09559.1"
                     /translation="MSKKRNKRRSKKKKVHHSLKVIPLGGLKEIGKNMTVYEYGNDII
                     IVDAGIMFPEEDLLGVDVVIPDLTYLENNAKKIRGIVFTHGHEDHIGAIP"
     gene            complement(276..716)
                     /locus_tag="VFD08_00695"
     CDS             complement(276..716)
                     /locus_tag="VFD08_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZK09560.1"
                     /translation="MKNSPVLTSLKENGYKLTEPRRRIAQVLESTKVHYRIEELYDMV
                     NQDGFLISQATIYRTVRLLEELNLLAKIDLGDGLDRYELRVASHHHHHLLCYHCKQLV
                     EVQGDFLDELEEQLEKLYDFRIEDHDVIFYGTCSQCKKRMEKNE"
     gene            complement(718..981)
                     /locus_tag="VFD08_00700"
     CDS             complement(718..981)
                     /locus_tag="VFD08_00700"
                     /inference="COORDINATES: protein motif:HMM:NF018633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HZK09561.1"
                     /translation="MTEKNCNCTDHNDNLESCEIELIDEEGNIVSFELEEWFDYNNKI
                     YAVLSDGEDAVILESVEEDGECCFITPTEEEFEKVKAYYEELE"
     gene            complement(984..1421)
                     /gene="ruvX"
                     /locus_tag="VFD08_00705"
     CDS             complement(984..1421)
                     /gene="ruvX"
                     /locus_tag="VFD08_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZK09562.1"
                     /translation="MVILALDYGEKRIGVALSDADESFVFSRPFIKRQGNAQAISKII
                     ELIAKEDIGLILLGAPYNMDDSISETMEKVLLFKKSLQKKINYTTRLIKRPSVVLWDE
                     RNTTTEARRILLKQDVSRKKRKEVVDSLAASILLEDYLRHKGA"
     gene            complement(1421..2335)
                     /locus_tag="VFD08_00710"
     CDS             complement(1421..2335)
                     /locus_tag="VFD08_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZK09563.1"
                     /translation="MNKVSKLCFGALTISPLQQNFTPEEAADLMEYAFNKGINFFDTA
                     EIYESYHLFRAFLKRTIAREDVFISTKCYAYDYKTAEASLNQALEEMQTPYIDLMMLH
                     EQESEHSLRGHNEALQFFDLMKKEGKIRHIGMSTHFVEGVLAGSNHQLIEVIHPIINK
                     RGLGIIGGNREDMENALKVAKEKGKFIFAMKVLGGGHLLKDYSEALCYAQDLPWIDSI
                     AIGMQSKEEVDANVSAILKRERISSGVYKKRKLIIDPYCIGCGKCVDRCQQKALYLDK
                     GKAKVLNERCILCSYCVGACEDFYIKVV"
     gene            complement(2328..2591)
                     /locus_tag="VFD08_00715"
     CDS             complement(2328..2591)
                     /locus_tag="VFD08_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HZK09564.1"
                     /translation="MGGAMNNHTQRFNNLDLKSQATREILSKVHEALVEKGYNPINQL
                     VGYLISGDPTYITSHNDARIIIKQVDRDEILEVLLKSFIEKNE"
     gene            complement(2588..5218)
                     /gene="alaS"
                     /locus_tag="VFD08_00720"
     CDS             complement(2588..5218)
                     /gene="alaS"
                     /locus_tag="VFD08_00720"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZK09565.1"
                     /translation="MKARSSTEIRKIFLDFFKSKGHTVMPSYSLIPEDDPSILLVGAG
                     MAPLKKYFSGQARPPSNRMATCQKCVRVGDIEEVGKTARHLTFFEMLGNFSFGDYFKK
                     ESLAWGIELLVDQLGIPKEKLWASVYEEDDEAYEIWEKDLNFPKERIVRLGKEHNFWE
                     IGLGPCGPSSEIYYDRGEAYGCGDPNHKPGCDCDQYIEVWNHVFTQFDKLEDGSYALL
                     NTKNIDTGMGLERLAMVLQEKDSVYEIDLMQELLSLISKITHKDYPSDETTSMRMRII
                     ADHARSMTFLISDGVVPSNEGRGYVLRKLIRRASLQLYLLGQEERHLSRLGKKSIELY
                     KEGYSELEQREDTILHMIDLEEDKFHQTLRRGMLYLEEITEQMKEEKLDILDGARAFK
                     LYDTFGFPLSLTEEILEEENFSVDIEGFDKHMKKQQEASRAQVDLDHIGWKDDLQKQL
                     TSLPKTEFVGYDGLVATGKVLEIISDNELKSVIEAGEKGQIILDKTPFYATGGGQVGD
                     WGYIESEHNLLNVQTVEQKEHLYLHKIDLLRGELRKGDQVTMVVDKVQRQATARNHSA
                     THILHKALIKVLGEGIQQAGSLVDENRLRFDFNYFEPLTKEQITQIEYLVNKDILDDH
                     IVEVSHSELDEAKKRGAKALFSEKYDQEVRIVKIGDSIELCGGTHVDQSAQIGLFVLL
                     AERGIASGVRRIEAITGTKALEYVNIQRNILDQAASLLKGNEEDILPKIEQLQEEKKK
                     LQQEIRQLKDQAGNDIIKDMISDKKTFDGFNLYLKKFKDQSMDGLKQLSDQLQAKDSQ
                     GLILLFSSQDDKIAIVSAAGDKARESGYKANRIVSKIAKQLGGGGGGREHFATAGAKD
                     LEKLNDVISNINHILVEVKE"
     gene            complement(5432..6463)
                     /gene="mnmA"
                     /locus_tag="VFD08_00725"
     CDS             complement(5432..6463)
                     /gene="mnmA"
                     /locus_tag="VFD08_00725"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZK09566.1"
                     /translation="MSTKVILGISGGVDSAVAAHLLISEGYDVSGVYLYLHQDAAEGI
                     DEAKSICEQLQIPFILIDGRELFRQRIINAFIETYQRGETPNPCVNCNETVKFKLLLE
                     EANRLGASFVATGHYAQIDKKERFLLKRAKDLTKDQSYMLYRLKQDQLSRILFPLGSL
                     TKDEVRQIARSIHLPQFEKKDSQDICFIPSGDHLAFLEERITLNTGNFIVDKKTIGPH
                     KGIEAYTLGQRRGLGISYSHPLYVQRIDPDTSEIYLDKEESLFSTLVEARELNFIYQI
                     LSLDEEVELMGKTRYSQTEFPCKVRRIEEDLLRVTFSKPQRAPTPGQSLVLYKDDYIF
                     AGGIIKETS"
     gene            complement(6453..6875)
                     /gene="nifU"
                     /locus_tag="VFD08_00730"
     CDS             complement(6453..6875)
                     /gene="nifU"
                     /locus_tag="VFD08_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HZK09567.1"
                     /translation="MYNDVVMDHFMNPRNVGEIENPDGVGEVGNIRCGDIMKIFLRIN
                     EEEVVEDIKFKTFGCGSAVASSSVATEMIKGKTVTEALKITNKDVLKELGGLPVIKVH
                     CSVLAEQALKAAVLDYANKRNKHYPELDGFDPDTEHEH"
     gene            complement(6883..8049)
                     /gene="nifS"
                     /locus_tag="VFD08_00735"
     CDS             complement(6883..8049)
                     /gene="nifS"
                     /locus_tag="VFD08_00735"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HZK09568.1"
                     /translation="MRKIYLDNSATTQIKPEVLEVMIDALINHNTNAASPHSYGQEGK
                     IDLDRSRETIANFLGAKTREIYFTSGGTESDNWAVHIAKKTKSGKHMITTTIEHHAMI
                     HTMEEAEKEGYDVTYLPVDEYGLVTAKQVEEALREDTILISINYANNEIGTIMPIEEI
                     GEVAKAHNILFHTDAVQAVGNVPIDLSNSNIDMLSFSSHKLYGPKGIGGLFIRSTVRP
                     YALIHGGAQERGYRGGTDNLPSIIAFSKACELAGENMPGHIERLSTLRDRLIEGILTK
                     VPHCKLNGHPSKRLPGNVNISFKFIEGESILLMLDMMGIAASSGSACTSGSLDPSHVL
                     MGIGLSHEIAHGSLRMTLGHFTREEDIDTVLEKLPPVIQKLREMSPLYEEEMRK"
BASE COUNT         2310 a         1740 c         1362 g         2776 t
ORIGIN      
        1 aaggaattgc accaatatgg tcttcatgac catgggtaaa aacaattcca cggatttttt
       61 ttgcgttgtt ttcgaggtaa gttaaatcag gaataacaac atccacacct aataggtctt
      121 cttcgggaaa cattatgcca gcatcgacga taatgatgtc attaccgtat tcatacactg
      181 tcatattttt accgatttct ttaagaccac ctaaaggaat aactttaagt gaatggtgga
      241 ccttcttttt cttacttcgt cttttgtttc tttttttact catttttctc cattcttttt
      301 ttacactggg agcaagttcc ataaaaaatt acatcatgat cttcgatacg aaaatcatac
      361 agtttttcta attgctcttc cagttcatct agaaaatccc cttgcacttc cactaattgt
      421 ttacagtgat aacaaagcaa atgatgatgg tgatgactag cgaccctaag ctcatagcgg
      481 tccagtccat cacccaaatc aatttttgca agcaaattta gctcttcaag cagtctaact
      541 gttcgataaa tcgtagcctg actgattaaa aagccatctt gattgaccat atcatagagt
      601 tcttcaatac gatagtgaac cttggtgctc tcaagaactt gagcgattct tcgccttggt
      661 tcagtcaact tataaccgtt ttcttttaga gatgttaata caggggagtt tttcatatta
      721 ttctaactct tcgtagtaag cctttacttt ttcaaactct tcttccgtgg gagtaataaa
      781 acaacattcg ccgtcttctt cgacactttc taaaataacg gcatcttccc catcacttag
      841 aacagcataa attttattat tataatcaaa ccactcttcg agctcgaagc ttacaatgtt
      901 accttcttcg tcgataagtt caatttcaca agactcaaga ttatcattat gatctgtaca
      961 attgcaattt ttttctgtca tctttatgct cctttatgcc ttaaataatc ttctagtaaa
     1021 atgcttgcag ctaaactatc aacaacttct tttctttttt ttctagagac atcttgtttt
     1081 aagaggattc tcctagcttc tgttgttgta ttgcgttcat cccaaagcac cacagaaggt
     1141 cgttttatca gtctagtcgt atagttgatt tttttctgga gagacttttt aaagagtaac
     1201 accttctcca tggtttcgct gatgctatca tccatattat agggagcgcc taggagaatt
     1261 agtccaatat cttctttggc aattagttcg ataatcttgc taattgcttg tgcgttccct
     1321 tgcctcttta taaagggacg actaaagaca aaagactcat cagcatcact aagagcaact
     1381 ccaattcgct tttcaccata gtctaaggcc aaaataacca ttataccacc ttaatataaa
     1441 aatcttcaca ggctcccaca cagtagctgc aaagaatgca gcgctcattt agtacctttg
     1501 ctttgccctt atcaagataa agggcttttt gctgacatcg atccacacat ttgccacagc
     1561 caatgcagta gggatcaata ataagctttc ttttcttata tactccagaa cttatccttt
     1621 ccctctttaa gatagcagat acatttgcat caacttcttc ttttgattgc attcctatag
     1681 caatagaatc aatccaagga aggtcttgag cataacaaag agcctcggaa tagtctttta
     1741 atagatgacc tccaccgagg accttcatag caaagataaa tttccctttt tctttggcaa
     1801 cctttagagc attttccatg tcttctcggt taccgcctat aatacccaga ccccttttgt
     1861 ttattatggg gtgaattact tcaataagct ggtgattgct tcctgctaga acaccttcta
     1921 caaagtgggt actcatacca atatggcgta ttttgccttc ttttttcatt aagtcaaaaa
     1981 actgcaaggc ttcattatgc cctcttaagc tatgctcact ttcttgctca tgaagcatca
     2041 taagatcaat ataaggtgtc tgcatttctt ctaaggcttg attaagtgaa gcttcggctg
     2101 ttttataatc ataggcatag cactttgttg aaataaaaac gtcttctcta gcaattgtcc
     2161 ttttcaaaaa agccctaaaa agatgataag actcgtaaat ttctgcggta tcaaagaagt
     2221 ttatgccttt attaaaggca tactccatca aatcggccgc ttcttctggt gtgaagtttt
     2281 gttgaagagg actaattgtt aaggctccaa agcataattt agaaacctta ttcattcttt
     2341 tcaataaaac tctttagaag aacttctaaa atttcatcac gatcgacttg tttaattata
     2401 atacgggcgt cattatgact ggttatataa gttggatctc cagaaataag ataacccaca
     2461 agttgattta tgggattata ccccttttca accaaggcct catgaacttt actcaggatc
     2521 tcacgtgttg cctgactttt taaatcaaga ttattaaagc gttgggtatg gttattcatg
     2581 gctcctccta ttcttttact tccaccagaa tatggttaat attactgatg acatcattta
     2641 gtttttctaa gtcttttgcc ccggctgttg cgaagtgctc tcgtccgcct ccaccacctc
     2701 cgagttgttt tgctatttta gaaacaatcc tatttgcttt ataccctgat tctcgcgctt
     2761 tatcacctgc agcgctaaca atggcgatct tatcatcttg actagaaaaa agaagtataa
     2821 gtccctgtga gtctttggct tggagttggt cagacaattg ctttaatcca tccatacttt
     2881 gatccttaaa ctttttaaga taaaggttaa aaccatcaaa agtttttttg tcggaaatca
     2941 tgtctttaat tatatcattt ccagcctgat ctttcaattg acggatttct tgctggagct
     3001 ttttcttttc ttcttgtaat tgttcaattt taggtaggat atcctcttca tttcccttaa
     3061 gaaggcttgc ggcttgatca agaatattgc gttgtatatt tacatattct agggctttag
     3121 tgcctgtaat cgcttcgatg cgtcgaactc ctgaagcaat ccctctttca gctaatagga
     3181 caaacaatcc aatttgggca ctttgatcta catgggttcc accacaaagt tcaatagaat
     3241 cccctatttt tacaattctt acttcttggt catatttttc actaaaaaga gcctttgcac
     3301 cacgtttttt tgcttcatct agttcactat ggcttacttc gacaatatga tcatctaaga
     3361 tatccttatt aactaaatat tcaatttggg taatttgttc tttagtaaga ggttcaaaat
     3421 agttaaaatc aaagcgaagt cggttttcat caactagaga acctgcctgt tgaataccct
     3481 caccaaggac ttttatcaag gccttgtgga gaatgtgggt ggcactatga ttacgagctg
     3541 ttgcttgtct ttgcacttta tcaaccacca tggttacttg atcgcccttg cggagttctc
     3601 cacgaagcaa gtcaatttta tgaaggtaca aatgctcttt ttgttcaaca gtttgaacat
     3661 ttagtagatt gtgctcactt tcaatatatc cccagtctcc tacttgacca ccaccagtag
     3721 cataaaaagg tgttttatct aaaatgattt gacccttttc ccctgcttca ataacggatt
     3781 taagttcatt atctgaaatg atttctagta cttttcctgt agcaacaagg ccatcatagc
     3841 ctacaaattc tgttttcgga agactggtaa gttgtttttg taaatcatct ttccacccta
     3901 tatgatcaag atccacttgt gctctagagg cttcttgctg ttttttcata tgcttatcaa
     3961 agccttcaat atcgacagag aaattctcct cttctaggat ttcttcagtt aaactcaagg
     4021 ggaaaccaaa ggtgtcatag agtttaaaag cccttgcacc atcaaggata tctagctttt
     4081 cttctttcat ttgctcagta atttcttcta ggtacaacat gccacggcgc agggtttgat
     4141 ggaacttatc ttcttcaaga tctatcatat gcaaaatggt atcttctctt tgttctagtt
     4201 cactgtagcc ttctttatat agttcaatgc tttttttacc caggcgggac aaatgacgtt
     4261 cttcttgtcc caaaaggtaa agctgtaaac tggccctgcg aattagtttg cgaagaacat
     4321 agcccctacc ctcattacta ggaactacgc catcactaat aagaaaagtc atgcttcttg
     4381 catgatctgc aataatacgc atacgcatac ttgttgtctc atcagaagga tagtctttat
     4441 gggtaatttt acttattagg ctgagcagtt cttgcataag gtcaatttca tagacagagt
     4501 ctttttcctg taataccatg gcaagacgtt caagtcccat gccggtatca atattttttg
     4561 tattaagaag agcgtaacta ccgtcttcta atttatcaaa ttgcgtaaaa acatgattcc
     4621 aaacttcaat atattgatca caatcacagc ctggtttatg gtttgggtca ccgcagccat
     4681 aagcctctcc cctatcataa taaatttctg aggaaggacc acagggacca agtccaattt
     4741 cccaaaaatt atgttctttg cctaggcgta caatacgctc ttttggaaaa tttaaatctt
     4801 tctcccaaat ttcataggct tcatcgtctt cttcataaac actggcccaa agcttctctt
     4861 tagggatgcc cagttggtca acaagaagct caatgcccca ggctagactc tcttttttaa
     4921 aataatcgcc gaaggaaaaa ttacccagca tttcaaaaaa agtaagatga cgagctgttt
     4981 ttcccacttc ttcaatatct ccaacacgaa cacatttttg gcaagtagcc atccgattac
     5041 tcggcggtct ggcctggcca gaaaagtatt ttttcaaggg tgccatacct gcacccacaa
     5101 gaaggatgct tggatcatct tcaggtatta gactatagct gggcataaca gtatgcccct
     5161 tggatttaaa aaaatctaaa aagatttttc taatctcagt actggatcgg gctttcattt
     5221 ctcctcctaa aaaaaagaag ccttctcaaa gataggaacg cctcggcgtt gtaccaccct
     5281 atttgtttcc tggataggaa tcactctgag aggctcaata agttattttc cttgtatttg
     5341 ataataaatc gaatatatag attgaactca tatctctaag acattccata tcctcagaag
     5401 atcagaaata ttgtagcatg aaatcaagta tttagctagt ttctttgatg attccaccag
     5461 caaaaatata gtcatcttta tatagtacca aggattgtcc cggtgtgggg gccctttgag
     5521 gcttgctaaa agtgacacgt agtaaatctt cttctatacg tcttacttta catggaaatt
     5581 ctgtttggga atagcgagtt ttacccatta gttcaacctc ttcatctaaa gaaaggattt
     5641 gatagataaa atttagctct ctggcttcta caagagttga aaataaactt tcttctttgt
     5701 ctagatatat ctcagaagta tcaggatcaa tcctttggac gtaaagagga tggctatagc
     5761 ttattcccaa gccccttctt tggccaaggg tataggcttc gataccttta tgggggccta
     5821 tagttttctt atcaactata aagtttcctg tattaagagt aattctctct tctaaaaagg
     5881 ctagatgatc tccactgggg ataaagcata tatcttggct gtcttttttt tcaaattggg
     5941 gtaaatgaat agaacgggct atttgtctaa cctcatcttt tgttagacta cctaagggaa
     6001 agagaatcct tgacaattga tcttgcttta gtctataaag catatagctt tgatcttttg
     6061 tcaaatcttt tgctcttttt agtaagaaac gctccttttt gtcaatttga gcataatgtc
     6121 cagttgccac aaagctggct cctaacctat tagcctcttc taaaagaagt ttaaacttaa
     6181 ccgtctcatt gcaattaaca caggggtttg gagtttcccc tctttgatag gtttcaataa
     6241 aggcattaat aatcctttgc ctaaaaagtt ctctgccatc tatgagaata aaaggaatct
     6301 gcaattgctc acaaatagat tttgcctcat caatcccttc agcagcatcc tggtgcaggt
     6361 ataaataaac accactaaca tcgtatccct ctgatataag aaggtgtgca gcaacggctg
     6421 aatctacccc tccactaatt cccaaaatta ctttagtgct catgttcagt gtcaggatca
     6481 aatccatcta attcaggata atgtttattt cttttatttg cataatcaag aacggcagct
     6541 ttaagagcct gctctgctag aacagaacag tgaactttga ttactggtaa accaccaagt
     6601 tctttcagga catctttatt ggttatcttt agagcttctg ttactgtttt tcccttaatc
     6661 atctcggttg ctactgaact tgaggcaaca gcacttccac agccaaaagt tttaaatttt
     6721 atatcttcaa ctacttcttc ttcattgatt ctaagaaaaa ttttcataat atcaccgcaa
     6781 cgaatatttc ccacctcgcc aacaccgtcg gggttttcga tttcaccaac attgcgggga
     6841 ttcataaagt gatccatcac cacatcatta tacattgcca ttttatttcc tcatttcttc
     6901 ttcgtataga ggtgacatct ctctaagttt ttgtattacc gggggaagtt tttcaagaac
     6961 agtatcaata tcttcttccc ttgtaaagtg ccctaatgtc atacgtaatg agccatgggc
     7021 aatttcatga ctaagaccta tacccataag aacgtgggaa gggtccaagc ttccgctagt
     7081 gcaggctgag ccactgcttg cagcaatgcc catcatatca agcattaaaa ggatactttc
     7141 tccttcaata aatttaaagc tgatatttac attacctggc agtcttttgc tgggatgccc
     7201 attgagttta caatggggaa cctttgtaag aatgccttct attaatctat ctcttagtgt
     7261 acttaggcgc tcaatatgcc caggcatatt ttcacccgct agttcacagg ctttagaaaa
     7321 agcaataatg gaaggaagat tatcagtgcc accacgatat cctctttctt gtgctcctcc
     7381 atggatcaag gcatagggcc gcaccgtact acggataaaa agcccaccta ttccctttgg
     7441 tccatataat ttatgggagg aaaaactcaa catatcaata tttgaatttg aaagatcaat
     7501 gggtacattt ccaaccgctt ggacagcatc tgtatgaaaa agtatattat gagccttggc
     7561 aacttctcct atttcttcta tgggcataat tgtaccaatt tcattattgg cataatttat
     7621 agaaattaaa attgtgtctt cacgaagggc ctcttctact tgtttagctg taacaagacc
     7681 atactcatct acaggaagat aggtaacatc atacccttct ttttctgctt cttccatggt
     7741 gtggatcatg gcatggtgct ctatagtagt ggtaatcata tgtttaccac ttttcgtctt
     7801 tttagcaata tgaacagccc agttgtcaga ttctgtgcca ccagatgtga aataaatctc
     7861 cctagtttta gccccaagga agtttgcaat cgtttctctt gacctatcaa ggtcaatttt
     7921 tccttcctgg ccataactat gaggactagc ggcattggtg ttatggttga ttagagcatc
     7981 aatcataact tctaaaactt caggttttat ctgagttgtg gctgagttgt ctaaataaat
     8041 ttttctcata tttctttatc ctttttaaca agatcgtcta aggtggttgt gttgacaata
     8101 tccattacat ggtgctctat acgattccaa aggggtcggg taaggcaatc ctttggattt
     8161 tcacaaacgt cgctacattc tttacccg
//
LOCUS       DASRNA010000025         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_604129,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000025 DASRNA010000000
VERSION     DASRNA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604129"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..593
                     /gene="pyrE"
                     /locus_tag="VFD08_00740"
     CDS             18..593
                     /gene="pyrE"
                     /locus_tag="VFD08_00740"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZK09569.1"
                     /translation="MERTLELFKESGALLKGHFLLSSGGHSDGYVQCAKLIMHPEKCQ
                     EVAEIMASKLTGQSFDLVVGPAMGGIIIAYEVARALGLPAIFTERVDGRMTLRRGFQI
                     KPGMKILIVEDVVTTAKSSMESAKVIEDLGGEVTALASIVNRTGGKKIPLPLYSAIKL
                     DIQTFPAQDCPLCAQNLKLVKPGSRSQKLSL"
     gene            625..1452
                     /locus_tag="VFD08_00745"
     CDS             625..1452
                     /locus_tag="VFD08_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK09570.1"
                     /translation="MDLRLTVLCENSVRLIGPLAEHGWSVFIETSQGNYLFDTGGGLS
                     LEHNAKFYKKDLSSLAGILLSHHHYDHTGGLKAALELSGPTQIYTHPHLFKESYSIRK
                     KKKLFIGMPYTRSYLENLGARWDFSKSFRELAPNIFLSGEIKRKTEYESGDKTLFVEN
                     QGQLSKDPLMDDQSLIFKRKEGLVIVLGCAHAGIINTLEHIIEKTGEKRILALIGGTH
                     LGSVSKEQKEKSLAALRNYQIDKIGVSHCTGTDASFALRQIYKDQFFHCEVGSVLEF"
     gene            complement(1532..2218)
                     /locus_tag="VFD08_00750"
     CDS             complement(1532..2218)
                     /locus_tag="VFD08_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09571.1"
                     /translation="MKKLTLVALIILLSFSLLSCGEGELSPLSTAGEEVDYSDKTPLK
                     KILEEKLKTDIDLTEDQLQAFRFKIIELVHAEDEKALLEEAGRIEEDYKEDLALREVY
                     ALLFYKAKFEGSFGSDDYPLAQDATPRIKFVLEAEDIERGSITEALKTVGIISSATEE
                     DLQQARKKMVTYFREKDAEVSAAEDEKFLEEIESMNPLDSMAYLQVRNIAKKEAFFGS
                     EKYPLNEEGD"
     gene            complement(2232..2795)
                     /locus_tag="VFD08_00755"
     CDS             complement(2232..2795)
                     /locus_tag="VFD08_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HZK09572.1"
                     /translation="MAIVELFLLALALSMDAFTVSICKGLSLKKARLKDMLYAGIWFG
                     GFQGLMPVVGYFFGRFFRGYMSPFSHGIAFVLLVLIGANMIRESYQDDCCEIGASMSA
                     KTMFLLAIATSIDALAVGISFAFLEIQIFGAALFISIITFVLSALGLKLGSVFGGKYK
                     AKAQFLGGVVLILIGVKTLLNGLGLIF"
     gene            complement(3006..3764)
                     /locus_tag="VFD08_00760"
     CDS             complement(3006..3764)
                     /locus_tag="VFD08_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZK09573.1"
                     /translation="MYDLSDKTVIITGGGSGIGLGIARAFSKAGANLVLTGRTHSKLD
                     NAKEELSGRGGDVFIITADGSKEEDVKAVIDAAIRKFGRIDVIINNAQNSASGVLLKD
                     HTEADFDRAIYSGLYATFYYMKHSYEALKEAKGRVINFASGAGISGKSGQSSYAAAKE
                     GIRGLSRVAATEWGADGITVNVICPLAMTEGLANWKLAYPELYEKTISATPMGRFADP
                     EADIGALCLFLSSKDASFITGETFHLQGGSGLRP"
BASE COUNT         1129 a          848 c          706 g         1112 t
ORIGIN      
        1 gattataagg taaatatatg gaaagaacat tggaattatt taaagaatct ggcgccctat
       61 taaagggaca ctttcttctt tcttcaggtg ggcattctga tggctacgtg cagtgcgcaa
      121 agctcattat gcatccagaa aaatgccaag aggtggcaga gattatggct agcaaactaa
      181 caggtcaatc ctttgatctt gtggtaggcc cagccatggg cgggattatt atcgcctatg
      241 aagtggccag ggccctaggc ctgcctgcta ttttcacaga aagagtggat ggaaggatga
      301 ccctaagacg gggttttcag ataaaaccag gaatgaagat cctaattgtt gaggatgttg
      361 ttactacagc taaaagctct atggaaagtg ccaaagtaat tgaagatcta ggaggagagg
      421 tcacagctct tgcttctatt gttaatcgca cagggggaaa aaagattcct cttcccctct
      481 acagtgccat aaaacttgat attcaaacct ttcctgccca agactgccct ctttgcgccc
      541 aaaatcttaa actggtaaaa cctggcagtc gctcacaaaa gttatccctt taggataact
      601 tttttcatct ataaggaggc tctaatggac cttagattaa ctgttttatg cgaaaatagt
      661 gttaggctaa tcggacccct agcagaacac ggatggtcag tttttattga aacaagccag
      721 ggcaactatc tttttgatac aggtgggggc ttaagtctgg agcacaatgc aaagttttat
      781 aaaaaggacc tttcttccct agctggcatc ctcctaagcc accaccacta tgatcacacc
      841 ggaggactta aagccgcact cgagctaagt ggaccaacac aaatttatac acatcctcac
      901 ctctttaaag aaagctactc catcagaaag aagaaaaaac tctttattgg catgccctat
      961 actaggagtt atttagaaaa tcttggagcc aggtgggatt ttagcaaatc ctttcgggag
     1021 cttgccccta atatctttct tagtggcgaa ataaaaagaa aaacagagta tgaaagcgga
     1081 gacaaaacac tctttgtaga aaatcaggga caactttcta aggaccctct tatggatgat
     1141 cagagcctta tttttaaaag aaaagagggc cttgttattg tcctgggttg tgcccacgct
     1201 ggcatcatca atacgctaga gcatatcatt gaaaaaacag gagaaaaaag aatccttgcc
     1261 cttattgggg gaacccatct tggctctgtt tcaaaagagc aaaaagaaaa gagcctagcg
     1321 gctcttagaa attaccagat agacaaaatt ggtgtttccc actgtacggg aacagacgct
     1381 tcttttgcac ttagacaaat atataaagac cagttcttcc actgtgaggt aggaagtgta
     1441 ctggagtttt aagactaaag agctctagca agggctcctg agcataaaag atatttactg
     1501 cggcgtgacg cttatcacgt cgtttttcta cttagtcccc ttcttcatta agaggatatt
     1561 tttctgagcc aaagaaggct tctttttttg caatattacg aacttgaaga taggccatgg
     1621 agtctaaggg attcatactt tcaatttctt ctaggaattt ctcgtcctca gcagcagaga
     1681 cttctgcatc cttttctcga aagtaggtga ccattttctt tcttgcttgc tgcaagtctt
     1741 cttctgtcgc actgctaata atccctaccg tttttaatgc ttctgtgatg ctccctcttt
     1801 caatatcttc tgcttctaag acaaatttta tcctaggagt cgcatcctgg gcaaggggat
     1861 aatcatcaga gccaaaagag ccttcaaatt tcgctttata aaaaagcagg gcatatacct
     1921 ctcttaaggc aagatcttct ttgtagtctt cttcaatccg gcctgcttcc tctagtaggg
     1981 ctttctcatc ttctgcatga accagctcta ttattttaaa acgaaaagct tgcagctgat
     2041 cctctgttag gtcaatatcg gtctttaatt tctcttccaa gatctttttt aagggtgttt
     2101 tatctgaata atctacttcc tccccagcag tagaaagggg gcttagttca ccttcaccac
     2161 aagaaagcag ggaaaaacta agtagtatga ttagagcaac cagtgtaagt tttttcattt
     2221 aactcctcct atcaaaaaat cagacccagt ccatttagca gagtttttac cccaataaga
     2281 attaaaacca ctccacctag aaactgggct tttgccttgt attttccgcc aaaaacactg
     2341 cctagcttaa gaccaagggc ggataataca aaggtaataa tagaaataaa tagggctgcc
     2401 ccaaaaattt gtatttctaa aaaagcaaag gagatcccca ctgctagtgc atcaatgcta
     2461 gtggctattg ccagtaaaaa cattgtttta gcactcatag aagcgccaat ttcacaacag
     2521 tcatcttggt aggattctct tatcatattt gcgccaatta ggactaataa aacaaatgct
     2581 attccatggg aaaagggtga catatagccc ctaaaaaatc gtccaaaaaa atagccaacc
     2641 actggcatca agccctgaaa accgccaaac cagatacctg cataaagcat atcttttaat
     2701 ctggcctttt taagggacag ccccttacag atagatacgg taaaagcatc catcgataag
     2761 gctagggcaa gtaaaaacag ctctactatt gccatacatt ttccccaatc cttctttttc
     2821 ttttaaccta atcattcctt tttaggtctt tttacctaat aacttccttt attctactcc
     2881 tatttctgcc ttactttcta acattatagc atacaaattt tgagcaataa aaaacgatac
     2941 ctgaaaaaaa ccaagcatcg cttatttctc cttaactagt tttgctcgct tataaaaggt
     3001 tttatttatg gtcttaaacc actccctcct tggagatgga aggtttctcc tgtgataaag
     3061 gaggcatcct tagaggataa gaataggcaa agtgccccta tatcagcttc aggatcagca
     3121 aaacggccca tgggcgttgc tgagatcgtc ttttcataaa gttcaggata agccagtttc
     3181 caattagcaa gaccttctgt catggctaga ggacagatca catttactgt aatgccatcg
     3241 gctccccact ctgttgccgc aacacgacta agccctcgaa tgccttcttt tgctgcagca
     3301 tagctagatt ggcccgattt tcctgagata cctgcaccag aggcaaagtt aataactcga
     3361 cctttggctt cttttaaggc ttcataactg tgtttcatat agtagaaagt agcataaagc
     3421 ccggaataaa tggccctatc aaaatcagct tctgtatgat cttttaacag aacgcctgaa
     3481 gcagaattct gggcgttatt aataattaca tcaatccgac caaactttct tatggctgca
     3541 tcaataacag ccttaacatc ttcttcttta ctaccatccg ccgttataat aaatacatct
     3601 cccccacggc cactaagttc ttccttggca ttatctagct tactatgggt tctacctgta
     3661 agtacaagat tggctccggc tttagaaaaa gctcgagcaa tgccaagtcc aatccctgaa
     3721 cctcctcctg ttataattac tgttttgtcg cttaaatcgt acatattgtc cccctttttt
     3781 ccttctgttt tacct
//
LOCUS       DASRNA010000026        14144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_381281,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000026 DASRNA010000000
VERSION     DASRNA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381281"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFD08_00765"
     CDS             complement(<1..647)
                     /locus_tag="VFD08_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09574.1"
                     /translation="MEETLRIGKRSFFLSLAVLLVLMVFAVFLSINLPSGSFERIISE
                     GKEMLVPGSYQLVAKPQLPFYRYLSAPIEVLFSTDGPTVIVILLFLLILGGSFTMLLQ
                     SFMISMLMDRLSKRFEAKRFLLLFVISFFFMFSGASFGVFEELIILVPFCLALARRMG
                     WDSLVGLGISLLSAGLGFSAALFNPFTLGVAQKLADLPLYSATFFRIGVFLSVYI"
     gene            complement(795..1940)
                     /locus_tag="VFD08_00770"
     CDS             complement(795..1940)
                     /locus_tag="VFD08_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZK09575.1"
                     /translation="MKTRKLVMIPGPTPVVRSIQDQMGKDTVAFGDPAFVNDFKELLE
                     DLKSLWQSDGEVFVVAGSGTMAMEMGIANTLKAGDNLLIVSHGYFGDRFIELAQRKGI
                     NVDVIASPWGKIVPLEDIEKKLKEKTYQAMTVTHVDTSTGVCAPIEEIGELAKKYPQM
                     IYIVDGVCSTAAEPEYMDQMNIDILLTGTQKAFGVAPGLAIVWASKKALARKEEIGSI
                     PEYYVDFDKWLPIMKDTSKYFATPAINMIWALKESVRIIKEEGIENRYARHRKNAKAM
                     QAALEAMGFTILAEKEVRAVTLSNVIYPQGIDDLEFRNLLAEEGAIVAGGLGDYAGKM
                     FRIGHMGNIDKHDMVSAIAAIERTLYKLGKKENLGQGVKTLLEELLG"
     gene            2348..3106
                     /locus_tag="VFD08_00775"
     CDS             2348..3106
                     /locus_tag="VFD08_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZK09576.1"
                     /translation="MRLENKIVVLTGASSGIGYSVAKQFAREGATVIAMARRANKLEE
                     LKESTLDLAGKVIPMQGDVVSQEDVDRVVKSTLEEFGRIDVLINNAGIIDNYQSAGSV
                     EDETWQKVIDVNVTGIMRMTRAVIPSMVENKSGVILNTTSVGGFNGMRGGLAYVATKH
                     AVVGMSKNIGYTYQQDGIRCVAIAPGTYDTEIGTHATAPDMKTLNHLMAGFKLFPHSG
                     NPDDLAHLYVFLASDEAKFINGTLVVSDGGWTAF"
     gene            complement(3495..4199)
                     /locus_tag="VFD08_00780"
     CDS             complement(3495..4199)
                     /locus_tag="VFD08_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZK09577.1"
                     /translation="MGTNGIIKLPSVVRRESFCVHKVPIFSHLETEELRKISALVKKR
                     NYKAKETVFSIGEEIRGLYILRYGSLKMVRYSREGEEVIVETLKKGDFYGSDNFFQSS
                     RSKEEAITLEKTGVCIIDEKDLVDIIKKNPDIAIKIIQVLSFMQGNDRNLISILSEKD
                     VLKRIALFLLWQTEDTPERTILITQDDLAKRINTTNETVSRKIKVLREKGLVEMPGYG
                     KIEIKNREALSIFVNE"
     gene            complement(4181..4738)
                     /locus_tag="VFD08_00785"
     CDS             complement(4181..4738)
                     /locus_tag="VFD08_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK09578.1"
                     /translation="MRRKDREMDEKFALSLIDQADYGVLSFCKENKSYSRPLYFARNN
                     RTLYFHSATQGEKVKLLSEGMDVELVFVGDIRVPKLYKKEEVKERMEKEGPRLLLNKV
                     FTTEFSSTMARGKIRLVNAAKEKKEALRKISQGFTPDMMEFFDEAVEMSLDKVLIYAV
                     ELYEIRGKRKKFDEKGEEMKWGRME"
     gene            complement(4774..6003)
                     /locus_tag="VFD08_00790"
     CDS             complement(4774..6003)
                     /locus_tag="VFD08_00790"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HZK09579.1"
                     /translation="MAYYEKKIVQGLDWISQYAIDPRGGLTRLVFTEPWLKTQNLILR
                     TMKEKGFDAYFDEVGNVHIRLEGERKETLLIGSHIDTVIQGGKLDGQYGVLAGLLALE
                     EVKNKFGKPKYSIEVISMSEEEGSRFPFNFWGSKNIVGAVDKNVVKELVDEEGITFLQ
                     ALKKAGFGLKKEKEIKKHYKAYCELHIEQGGVLEDAGKKIGIVHTIVGQRRYEVYLKG
                     EANHAGTTPMSLRRDALDGAARMVLGLKDLARKYGDPMVATVGQISLEPGSSNVIPAQ
                     VRFSIDIRHSKKDLLEKFDKQMKELFKDISEEEGLEHTIILNEKSLPVPLDNDLQELL
                     EKLCTDKKISFMSMVSGAGHDAQNFAPYIPTALIFVPSIGGISHSPAEDTKLEDLVDG
                     LRLLENFIYRWAYEDKR"
     gene            complement(6240..6947)
                     /locus_tag="VFD08_00795"
     CDS             complement(6240..6947)
                     /locus_tag="VFD08_00795"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HZK09580.1"
                     /translation="MQTLKLLKETNKIVFFGGAGTSTESGIPDFRSAGGIYSKAPEEW
                     LSSTYLYRHSEDFYDFYKKTMLYPDAKPNRGHEILQEWEEKGKLLGIITQNVDGLHQK
                     AASKNVVEIHGSVERNYCELCRKRYSLQDVIDQEGIPYCSCGQMIRPDVVLYGEMLND
                     RVVQKATNLLRKAELLIVGGTSLVVYPAAGMLQYFGGDHIIMINKGSTSYDRRANLVL
                     RNGFSEDMIYLDKEMFK"
     gene            complement(7003..7554)
                     /locus_tag="VFD08_00800"
     CDS             complement(7003..7554)
                     /locus_tag="VFD08_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09581.1"
                     /translation="MKRRDKKKNLLIGVGVGLAMGAGVGTLLYKKYAHLKENSDFLFI
                     ADNKTVDFSNEDFEDVSVVAVASNILVDLSAAKADENPMTLYLKGMGSNVDVVVPEGW
                     NVKPQGTAKGSQLDNATDFDYDDIKAPLLFIDYDLRGTSFNVFYSFAQFEEEVVEELV
                     EELMEEAEEEVVEEVEEVEEVEE"
     gene            complement(7544..8641)
                     /locus_tag="VFD08_00805"
     CDS             complement(7544..8641)
                     /locus_tag="VFD08_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09582.1"
                     /translation="MRSIARIVIAIISLILGFITGQWILTSTDLAILFPSIFRATGFF
                     RILIHGLSTFIFAIILYILFSFLWKRGSKTVKNLAETIGEQPPLQIIGGSAGLLVGLI
                     AAFFVSNIYSSIFSPLSIILSVITYTFFATLGLIVGSKLAHPGDFPNIDFKKKASPKN
                     KKGAMPKVLDTSAIIDGRIVEISKTGFMEGEFVVPAFVLKELRHIADSSDDLTRAKGR
                     RGLDYINELKENNFITLNLTDRDFKDVDEVDVKLLHLATELKGAVITTDYNLNKIALL
                     QKIPVLNVNDLSNAVKPNALPGESFVIEIIKEGKEVEQGVGYLHDGTMVVIDQGKHYI
                     GQQRVVEVTSSLQTPAGRMIFAKIKEDQNET"
     gene            complement(8629..9120)
                     /locus_tag="VFD08_00810"
     CDS             complement(8629..9120)
                     /locus_tag="VFD08_00810"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZK09583.1"
                     /translation="MKIGSKIVYPMHGAGIVVDIEHEDFFGKRIEYCTMKVAQKDMTL
                     KFPLDRSENLHIRKVSNMEAIFGQIREKEPEEYIHSSNWNKRYQATLEKMKVGSTSDM
                     ATVLVNLKSLETIKGLSSGERQLYHTAMDILTSELMLIKDCEYEGAYKLLEQCLEDSI
                     CEV"
     gene            complement(9167..10498)
                     /gene="radA"
                     /locus_tag="VFD08_00815"
     CDS             complement(9167..10498)
                     /gene="radA"
                     /locus_tag="VFD08_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZK09584.1"
                     /translation="MKSQFVCQECGAIFPKWMGRCSECGSWNSLLEETIEKKKPTKGA
                     AVLATPISSIKIHEDSRVSSGIGELDRVLGGGIVEGSLILVGGDPGIGKSTLLLQVAH
                     HVAIRGKKVLYVSAEESQLQIKLRASRLKIKGEGIFLVSETSLEKILPTIQREKPDVL
                     IMDSIQTISTQALGSAPGSVSQVREITSVLMDLAKNQGIATFIVGHVTKSGTLAGPKV
                     LEHMVDTVLYFEGDGSSVYRILRSVKNRFGSTNEVGLFHMGSLGLLEVDNPSEMFLSN
                     ASGFEPGTVVFPSIEGTRPLLVEVQSLVSPSNFPSPRRMALGVDYNKLVLSVAILEKK
                     RNLFLQNQDIYVNAVGGIQIGEPALTLPMMVSIASSLLDQPLPKKMIILGEVGLLGEV
                     RNVPQIEKRLMEAKRLGYERAMVPKCNVKVEGMRIDEVSHVDQVFSILFTK"
     gene            complement(10538..12616)
                     /gene="fusA"
                     /locus_tag="VFD08_00820"
     CDS             complement(10538..12616)
                     /gene="fusA"
                     /locus_tag="VFD08_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZK09585.1"
                     /translation="MKQYASDKIRNVTLLGHGGSGKTSLLEAILFDKKVTDRLGSVDD
                     GTSVGDYSKEEIQRQFTIGTSIIPMEWAEGKINFLDTPGYFDFVGETIGALSISGGAV
                     IVIDASSGIEVGTEKAWNYTQQRNMPRILFINKMDKENINYVELMEKLKASFGMKIAP
                     FVVPIGDGEEFKGYVNVIDMVGRIIEGDKVLDAEIPEEIKDRVATVREMLIESVAESD
                     EELLEKYFEGEEFTEEEIRQGLRQGVLNGEVVPVVVGSASKQVGITMILDTIADYLPT
                     SLEARGGQVKAFKEGTEEEIEIAVDPDGPFSAQVFKTVVDPFIGKISLFKVISGSLKK
                     DMEVYNTNKESNERIGHVFYLRGKEQIDATVIGTGDIGAVAKLNVTQTGDTLCDKSRK
                     IQFPPIPFFSPGLFKAIQPKSKGDEDKIGTSLQRLNEEDPTFSITRNQETKQMIIGGQ
                     GTMQLLVLVDKLKEAYGVEVEMIPQKIAYRETIKGKVTQQGKYKKQSGGAGQYGDVII
                     DFEPSEGGFVFEEKVFGGAVPRNFFPAVEKGIEEALENGPLAGFPVVDLKATLLDGSY
                     HAVDSSEVAFKVAASLAFRDGLPKANPILLEPIMHLEITIPDDYMGDIMGDLNKRRGR
                     ILGMEPSEDGGQTVFAEAPEAELFSYSSDLRSMTQARGSFTVKFERYDEVPAMIAEKV
                     IEEVKKEEED"
     gene            complement(12777..>14144)
                     /locus_tag="VFD08_00825"
     CDS             complement(12777..>14144)
                     /locus_tag="VFD08_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-triphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HZK09586.1"
                     /translation="SGHSSLKNMFLKIDRVVKKAGLISDEQRIKLRPIDCLDIANIIG
                     ENVVVGGVRRTAEIVLLDQEDRESIEAKSKLYKQIDGQWIVDKELLHRSMSNNSIYYT
                     SKPSREMFSWQMEQMRYSGEPGWVNAEAGKKRRPNFEGVNPCAEILLDNKGLCNLTTV
                     NVAAFAREDGSLDMQGLLKAQKLSARAAFRMTQVELELSHWNHVHHRDNLIGTSLTGW
                     QDMVNACNLTQEEEADFLRKLRSVARSEAESYAAELDVEAPLLVTTIKPEGTLSQLPE
                     VSSGIHYSHSPYFLRRVRINATDPLVKVCEELGYPVHPEVGQEWDSCTTKVVEFPIKA
                     PEGRTKYDVGAIEQLENYKLFMENYVDHNASITVHVRDHEWEAVEEWVWEKWDDIVAI
                     SFLALDDNFYDLLPYESISKEEYEKRKAEMNPFRPSLISKYEVEEILFDIGDEGCETG
                     VCPIR"
BASE COUNT         3989 a         3186 c         2349 g         4620 t
ORIGIN      
        1 ataatataaa cagacaaaaa gacccctata cgaaaaaagg tggctgaata taagggaagg
       61 tctgctagtt tttgagccac acccaaagta aagggattaa aaagagctgc actaaagcca
      121 agtcccgccg aaagaaggct gattccaagg ccaacaagac tatcccagcc cattcttcgt
      181 gcaagagcaa gacaaaaggg aacaagtata attagttctt caaagacacc aaagctagca
      241 ccagaaaaca taaagaaaaa actaatgaca aaaagcaaaa gaaatcgttt tgcttcaaag
      301 cgtttactta gtcggtccat tagcatacta atcataaaag attgcaggag catggtgaag
      361 gaaccaccta aaataagtaa gaatagaagt ataacaatta ctgttggacc atctgtagaa
      421 aacaagacct caatgggcgc gctaagatat cggtaaaaag gtagctgcgg ttttgccaca
      481 agctgataac tgccaggaac cagcatttct tttccttcgc ttataatccg ttcaaaggat
      541 cccgagggta aattaatgct aagaaaaacc gcaaaaacca taagcactag cagcacagca
      601 agggaaagga aaaacgatcg tttccctatt cgaagggttt cttccatttt atacctcctt
      661 ttttctagat agaaatatag tattaacaca gaccaggaaa atcaaaataa tcagtggctt
      721 atatcccttt tttattctaa aactgggggt ctgataaaaa tcataccccc agctcttatg
      781 ctttttctct gtttttatcc aagaagttct tctaagagtg ttttaacacc ctgtccaaga
      841 ttttcctttt ttcctagctt gtaaagggtt ctttcaatag cagcgatggc acttaccata
      901 tcgtgcttat caatattgcc catatggcct attctaaaca tttttcctgc ataatcacct
      961 aggccaccag caactatagc tccttcttct gctagaagat tcctaaactc taaatcatct
     1021 attccctggg ggtaaataac attagataac gttaccgccc taacttcttt ttccgctaaa
     1081 attgtaaagc ccattgcttc cagagccgct tgcattgcct tggcattttt tctgtgccta
     1141 gcatagcgat tttcaatgcc ttcttctttg ataattctta ctgactcttt taaggcccaa
     1201 atcatattta ttgctggtgt agcaaaatat tttgatgtgt ccttcataat tggcagccat
     1261 ttatcaaagt cgacataata ctctgggatg cttcctattt cttcttttct ggctagggct
     1321 tttttgctgg cccaaacaat agctagacca ggggctacac caaaggcttt ttgtgttcct
     1381 gttagaagaa tatcaatatt catctgatcc atatactccg gctctgctgc cgtagagcaa
     1441 acaccatcaa caatataaat catctgtgga tactttttag caagctcgcc tatttcttcg
     1501 atcggtgcac aaacaccagt agatgtatca acatgggtaa ctgtcattgc ctgatatgtt
     1561 ttttctttca gcttcttttc aatatcttct agaggaacaa tttttcccca aggactagca
     1621 attacatcta cattgattcc ttttctttgg gccagttcaa taaatctatc tccaaaataa
     1681 ccatgcgaga caattaaaag attgtcacct gcttttaggg tgttggcaat tcccatctcc
     1741 atagccattg tgccacttcc tgcaacaaca aagacttcac catcactttg ccatagactt
     1801 ttaaggtctt ctaaaagttc cttaaagtca tttacaaaag ccggatcgcc aaaagcaacc
     1861 gtatcttttc ccatctgatc ttgtattgat cgtacaacgg gtgtaggtcc tggaatcata
     1921 actaattttc tggttttcat ttcttctcct taataaattt gtttcctaca aagacataaa
     1981 tcagtgtaag ctagtttctt ttaccttaaa tctaattgta ctttcttagg gtaaactgcc
     2041 tctaatgtaa gaaaatacca ccatttgtat caaggtaaat atcatttttt ctgctaaaac
     2101 tacaaagtga tctgctatca tcttatcacc tatatcttta ctagtggtaa atgactttac
     2161 ccttgttaaa ttaagtttag ttttttatgg tccttatgtc aagaattact atctaacttt
     2221 agccattttc ctctcttatt agggtcttat gtgggtaatt aatagttgtt aattttttgt
     2281 aaagcttatt tctcaaattt tatattatac ttgtgttaca cttatatgaa aaaatggagg
     2341 taaagttatg cggctagaaa acaaaatcgt tgtcctcaca ggtgccagct caggaattgg
     2401 ctattccgta gcaaaacaat ttgctaggga aggggccact gttattgcta tggcaagacg
     2461 ggctaataaa cttgaagaac taaaagaaag caccttagac cttgcgggaa aagtaattcc
     2521 tatgcaaggg gatgttgttt cccaagaaga tgtagaccgt gtcgttaaaa gtactttgga
     2581 agaattcgga agaattgatg tcctaattaa caatgcggga attattgata attatcaatc
     2641 tgccggcagt gtggaagatg aaacatggca aaaagttata gacgttaatg tcacaggcat
     2701 tatgcgcatg acaagagccg ttatcccttc aatggtagaa aataaatctg gcgttattct
     2761 taacacaacc tcagtaggtg ggttcaatgg aatgagaggc ggtctagcct atgtcgctac
     2821 caaacacgca gtagtgggaa tgtcaaaaaa cattggttac acctatcagc aagatggaat
     2881 tcgttgtgtc gctattgccc caggtaccta tgacactgaa attggaaccc acgctacagc
     2941 ccctgatatg aaaacactaa atcatctaat ggctggtttt aaactcttcc ctcactcagg
     3001 taatcctgat gatttagctc atctttatgt tttccttgca tctgatgagg caaaattcat
     3061 caacggtacc cttgttgttt ccgatggtgg atggacagct ttctaatatt ttgtatacta
     3121 aaaaattata gaagatatgg acctatttct aatacaaagc agtggttaaa atagccactg
     3181 cttttttcta gaaaaataga aaagaaaatc ccccctcctt ctagtctgcc accaggagct
     3241 agagcaaagc ttttgccaag gacagctctt gcctaggtga gcctaaatag ctgtcaagcc
     3301 cttgcctttg aggattctct gttctactaa ttgaaccagc ctctctaagg ttgcactttc
     3361 atctaatgcc caacttaaga gttttacagc aacttctctg cttctttctc cttttacaaa
     3421 agaagttggg ggcttattgc tcccttgagc tctttgagtt agaccttgga aaagctagtt
     3481 ttttttgacc cggcttactc atttacaaaa atagacaagg cctcccggtt ttttatttct
     3541 attttcccat aaccgggcat ttcaactaaa cctttttcgc gcagcacttt tatttttcta
     3601 ctgacggttt cattggtcgt atttattctt ttagccagat catcttgggt gatcagaata
     3661 gtccgctcag gagtatcttc tgtttgccaa agtagaaaaa gagcaattct ttttaagacg
     3721 tctttttctg aaagtataga aattaaattt cgatcatttc cctgcataaa ggatagtact
     3781 tgtatgattt tgattgcaat atcaggattc tttttaataa tatctactag atccttttca
     3841 tcaataatgc agacccctgt tttttcaagg gtaatggcct cttcttttga acgagaagat
     3901 tgaaaaaaat tatcactacc ataaaagtca ccttttttaa gcgtttcaac aattacttct
     3961 tcgccttctc tagaataccg aaccattttt agtgaaccat aacgtaaaat atataggccc
     4021 cttatttctt ctcctataga aaaaacagtt tccttcgcct tatagtttct tttttttact
     4081 agggcactaa tctttctgag ttcttctgtt tcaagatgag aaaaaatcgg cactttatga
     4141 acgcaaaaac tttctcttcg caccacacta ggtagtttta ttattccatt cgtccccatt
     4201 tcatctcctc acctttttca tcaaacttct tgcgtttccc tcggatttca tatagttcca
     4261 ctgcataaat aagcaccttg tccaaactca tttcaacagc ctcatcaaaa aactccatca
     4321 tatcaggagt aaagccttgg ctaatttttc ttagagcttc ttttttttct tttgcagcat
     4381 ttactaagcg aatcttcccc cgggccatag ttgaagaaaa ctctgttgta aagaccttgt
     4441 ttaataagag tcttgggcct tctttttcca ttcgctcttt tacttcttcc tttttatata
     4501 gtttaggaac tcgaatatca ccaacaaaaa caagctctac atccataccc tcgcttagga
     4561 gctttacctt ttctccctgg gttgccgaat gaaagtaaag tgttctatta tttcttgcaa
     4621 aatacagggg gcggctataa ctcttgtttt ctttacaaaa gctcaatacg ccatagtcgg
     4681 cctggtctat tagactaagg gcaaattttt catccatttc tctgtctttt cgtctcattt
     4741 ataccttcct ttcctctcat agtaaaatgt gttttatcgc ttgtcttcat aggcccacct
     4801 ataaataaaa ttttctagga gtctaagccc atctactagg tcctctagct ttgtgtcttc
     4861 tgctgggcta tggctaattc cccctatgga gggaacaaag atcaaggctg ttggaatata
     4921 aggagcaaag ttttgcgcat catgcccagc tcctgagacc atagacataa aggatatttt
     4981 tttatctgtg cataatttct ctaaaagttc ttgaagatca ttatccaagg gaacaggaag
     5041 agatttttca tttagaatta ttgtatgttc taagccctct tcctcgctaa tatctttaaa
     5101 gagctctttc atttgcttgt cgaatttttc aagtaagtcc ttcttactat gacgaatatc
     5161 aatactaaag cgtacttgag cgggaataac attacttgat cctggctcta agctaatttg
     5221 gccaaccgta gcaaccatgg gatcaccata ctttctagcc aggtctttaa gccccagcac
     5281 cattcttgct gctccatcaa gagcgtccct gcgtaggctc ataggggtcg ttcccgcatg
     5341 gtttgcttct cctttaagat agacttcata gcgcctttga ccaactatgg tatgaacaat
     5401 ccctatcttc ttacctgcat cttctaacac tccgccttgc tcaatatgga gttcgcaata
     5461 ggccttatag tgttttttta tttccttttc tttttttaag ccaaaacctg cttttttaag
     5521 agcttgaaga aaagtaatcc cttcttcatc aaccagttct ttgactacat ttttatctac
     5581 tgcaccaacg atatttttgg atccccaaaa attaaaggga aagcggcttc cttcttcctc
     5641 agacatactt attacttcaa ttgaatattt gggtttgccg aatttgtttt tcacttcttc
     5701 tagagccaaa aggccagcta aaacgccata ctgtccatcc aatttccctc cttggattac
     5761 cgtgtcaata tgacttccaa taagaagtgt ttcttttctt tctccttcca ggcgaatatg
     5821 gacatttcct acctcatcaa aataagcatc aaaacctttt tccttcatag tcctaagtat
     5881 aagattttgc gtttttaacc aaggctctgt aaataccagg cgagtaaggc caccacgtgg
     5941 atctatagca tattggctga tccaatccag tccctgaaca atcttttttt cataataggc
     6001 cataatagta tctcctttat aaacttctct aatcttcttt tagtataaca gataaggcaa
     6061 gcagcctctt gcaaaaaggc ctgggccact tggcaagtaa gtggtttttt tccttgctct
     6121 tttttccttt ttatctgcta tttaaaaagg caggcgcaaa atccgcctac ctctttatta
     6181 aaataataga acccatacaa ttttatataa gttttcacta gaaaaaaata gctgtgtttt
     6241 tacttaaaca tttctttatc aagatagatc atatcctcag aaaaaccatt tcttaaaaca
     6301 agatttgccc ttctatcgta agaagtactg cccttattaa tcattataat atggtctcct
     6361 ccaaaatact gcaacattcc cgctgctgga tacactacta aggaagtacc tccaacaata
     6421 agaagttctg cctttctaag tagatttgtt gctttttgaa ccaccctatc attgagcatt
     6481 tcaccataga gtaccacatc aggtcgaatc atctgtccac aggaacaata gggtatcccc
     6541 tcctgatcta taacatcctg cagggagtat ctcttcctgc aaagttcgca gtagtttctc
     6601 tccacacttc cgtgaatttc gacaacattt ttgctagcag ctttttgatg aaggccatcg
     6661 acattttggg taatgattcc caaaagcttt cccttttctt cccactcctg caatatttca
     6721 tgcccccgat ttggtttggc atcagggtag agcatagttt ttttataaaa atcataaaaa
     6781 tcttcagaat gtctatataa atatgtggaa gataaccatt cttctggagc cttactatag
     6841 atccctccag cggatctaaa gtctggtatt ccactttcgg tagaagttcc tgctccacca
     6901 aaaaaaacta tcttattcgt ttcttttagt aatttaaggg tttgcattct tcctcctctt
     6961 ttaaaaaaag acatcccttg atgtcttttc cttatttcta atttattctt ctacttcttc
     7021 tacttcttct acttcttcta ctacttcctc ttcagcttct tccattagct cttccactag
     7081 ctcttctact acttcttctt caaattgtgc aaaggaatag aagacattaa agcttgttcc
     7141 cctaagatca tagtcaataa aaagaagtgg agctttgata tcatcataat caaaatccgt
     7201 tgcattatct agttgacttc ccttagctgt tccctggggc tttacattcc aaccttcagg
     7261 aaccactaca tctacattgc ttcccatccc tttaagataa agggtcatgg ggttttcatc
     7321 tgctttggca gcagaaaggt caactaagat attgctggca actgccacta cagaaacatc
     7381 ttcaaagtct tcattagaaa agtctactgt cttattatca gcgataaaaa gaaagtccga
     7441 gttttctttc agatgagcgt actttttata gagtagggtt ccaacacccg cccccatagc
     7501 taaaccaaca cccacaccaa taagtaaatt cttttttttg tctctacgtt tcattctgat
     7561 cctccttaat tttagcaaat atcatgcgac ccgctggggt ctgcagagaa ctagtaactt
     7621 caacaacacg ctgttggcct atataatgtt taccttgatc gattacgacc atggtaccat
     7681 catgtagata accaacacct tgctctactt ctttaccttc tttaataatt tcgataacaa
     7741 aactttctcc tgggagtgca tttggtttaa cagcattgga aaggtcgtta acatttaaaa
     7801 cgggaatttt ctgtaacaag gcaattttat ttagattata atctgttgta ataacggctc
     7861 ccttaagttc agtagcaaga tgcaagagtt tcacatccac ttcatccaca tctttaaagt
     7921 cgcgatcagt tagattaaga gtaataaaat tgttttcctt cagttcattg atgtaatcca
     7981 gtccccgtct acctttagcc cttgtcaaat catcagagct atcagcaata tgcctgagtt
     8041 cttttaggac aaaagctggt actacaaact caccttccat aaaacctgtc tttgaaatct
     8101 ccacaatcct tccatctata atagcagatg tatctaaaac ttttggcata gctccttttt
     8161 tattttttgg cgaagctttt ttcttaaagt ctatattggg aaaatctcca ggatgggcca
     8221 gttttgaacc tacaattagt ccaagagtgg caaagaatgt ataagtaatc actgacaaaa
     8281 taatactaag cgggctaaaa atactactgt agatattact cacaaaaaag gctgcaataa
     8341 gtcccacaag caaaccggcg ctgcccccta taatttgtag gggtggctgt tcaccgattg
     8401 tttcagcaag gttttttact gtcttcgatc cacgtttcca taaaaagcta aataatatgt
     8461 acagtataat agcaaaaata aatgtgctaa ggccgtgaat taaaatccgg aaaaaacctg
     8521 ttgctcgaaa aatacttgga aataatattg ccaagtcagt ggatgttaat atccactgac
     8581 cagttataaa acctagtatg agcgagataa tagctataac aattcgcgct atacttcgca
     8641 tatagaatcc tccaaacatt gttccaggag cttataggct ccttcatact cgcaatcttt
     8701 gattaacatt agttcactgg taagtatatc catagctgtg tggtatagtt gtctttctcc
     8761 cgatgaaaga ccctttatag tctccagact ttttaagttt accagtaccg tagccatatc
     8821 agaagtgcta cctactttca ttttttctag tgtggcttgg tatcgcttat tccaatttga
     8881 tgagtggatg tattcttcgg gttctttctc tcttatctga ccaaaaatag cttccatatt
     8941 ggaaactttt ctaatatgca gattttcact tcgatctaat gggaacttca gcgtcatatc
     9001 tttttgtgcc actttcattg tgcagtattc aattcttttg ccgaagaagt cttcatgctc
     9061 tatatcaacc acgatgcccg ctccatgcat tggataaaca atcttcgaac cgattttcat
     9121 acctgcctcc ttctctatct atacccaatt gtaacatatc ctcactttac ttggtaaaaa
     9181 gaattgaaaa tacttgatca acatggctaa cttcatcaat acgcatgccc tctaccttca
     9241 cattgcactt aggaaccata gccctttcat aacctaggcg tttggcttcc atcagtcttt
     9301 tttctatttg tgggacattt cttacttccc caagcaggcc tacttcacca agtataatca
     9361 tcttttttgg tagaggctga tcaagtaagg aagatgctat gcttaccatc atggggaggg
     9421 taagtgctgg ttcacctatt tgtatacccc caacagcatt gacatagata tcttgatttt
     9481 gcaaaaaaag atttcttttc ttttcaagga ttgccacaga aagaactagt ttattatagt
     9541 ccactcccag ggccattcgc cgcggggagg gaaagttcga cggactaacc aagctctgga
     9601 cttcaacaag taggggcctg gtcccctcaa tagagggaaa tacaacggtt cctggctcaa
     9661 aacccgaagc attggacaaa aacatctccg aaggattatc cacttctaat aaacccaggg
     9721 atcccatatg aaaaagccct acctcattgg ttgaaccgaa tctgttttta acacttctaa
     9781 gtattcggta gacactggat ccatcacctt caaagtacaa tactgtatcc accatatgct
     9841 caagaacctt cggaccagct agtgtgccac ttttggtcac atgacctaca atgaaggtgg
     9901 cgattccttg gtttttagct aaatccataa gaacactagt aatctctcgc acttgagaaa
     9961 ccgatcctgg agctgagccc aaggcttgag tgcttatggt ttgtatagaa tccataatta
    10021 gcacatctgg tttttctctt tgaattgtcg gtagaatttt ttcaagggaa gtctcagaaa
    10081 caagaaaaat cccctctcct ttaatcttca aacgacttgc tctaagcttt atttgaagtt
    10141 ggctttcttc tgctgaaaca taaagtactt tttttcctct tattgcaaca tgatgtgcca
    10201 cttgaagcag cagcgtgctc tttcctatgc caggatcacc cccgacaaga ataaggctgc
    10261 cctcaactat tcccccgccc aaaacccggt caagctctcc aataccagaa ctcaccctac
    10321 tatcttcgtg gatttttatt gaactaatgg gggtggctaa gacagcggct cctttagttg
    10381 gttttttctt ttctatcgtt tcttctaaaa ggctattcca acttccacac tcactacagc
    10441 gccccatcca tttggggaaa atagctccac attcctgaca aacaaactga cttttcacaa
    10501 agcctcctta aaaaataaag gggctaagcc cctttattta atcttcttct tcctttttta
    10561 cttcttcaat aactttttct gctatcatgg ctggtacttc atcatatctt tcgaatttca
    10621 ctgtaaagct tccccgggct tgggtcatac tcctaagatc ggaagaatag ctaaaaagtt
    10681 ctgcctctgg ggcttcagca aaaactgttt ggcccccatc ttcgcttggt tccataccaa
    10741 gaatgcgtcc tcttctttta tttaaatccc ccatgatatc tcccatataa tcatcaggaa
    10801 tcgttatttc cagatgcata attggctcaa gtagaatggg atttgcctta ggaagtccat
    10861 ccctaaaggc caaggaggca gcaaccttaa aagcgacctc ggaagagtca acagcatgat
    10921 aactaccatc tagaagagtg gcctttaggt ctactacagg aaaacctgcc aggggaccat
    10981 tttccaaagc ttcttctatt cctttttcta cagcagggaa gaagtttctt ggtactgcac
    11041 caccaaacac cttctcctca aaaacaaaac ctccctcaga tggttcaaaa tcaataataa
    11101 catcaccata ttgacctgct ccaccagatt gttttttgta ttttccttgc tgggttactt
    11161 tacccttaat tgtttcacga taggcgattt tttgaggtat catttccacc tcaacaccat
    11221 aggcttcctt aagtttatcc acaagaacaa gaagttgcat ggttccttga ccaccgataa
    11281 tcatttgttt tgtttcttga ttacgagtaa tactaaaggt cggatcttct tcatttaatc
    11341 tttgtagaga tgttccaatt ttgtcttcat cacccttgct ctttggctgg attgccttaa
    11401 agagtcctgg tgaaaagaat ggaattggtg gaaactgtat ttttctactc ttatcacaaa
    11461 gagtatctcc tgtttgggtt acatttagct tggcaacagc accaatatca cctgttccaa
    11521 taaccgtggc atctatctgc tctttacccc ttaaataaaa gacatgacca atacgctcgt
    11581 tgctttcctt gtttgtattg tagacctcca tatctttttt aagactgccg gatataactt
    11641 taaaaagaga gattttcccg ataaaaggat ccacaaccgt tttaaagacc tgagcactaa
    11701 atggaccatc aggatcaact gctatctcga tttcttcttc tgtgccttct ttaaaagcct
    11761 ttacttggcc accacgggct tcaagagaag taggtaaata atctgcaatc gtatctaaaa
    11821 tcatggtaat gcctacctgc ttgctagcac tacctacaac aacaggaaca acctcaccat
    11881 ttaaaacacc ttgacgaagt ccctggcgaa tttcttcttc tgtgaattct tctccttcaa
    11941 aatacttctc aagaagttct tcatcacttt cggcaactga ctcaattagc atttctctta
    12001 ccgttgcaac tcgatctttg atttcttctg gaatttctgc atcgagaacc ttatcgccct
    12061 ctataatgcg tcctaccata tcaattacat tgacatagcc cttaaattcc tctccatcac
    12121 ctatgggaac aacaaaggga gctattttca ttccaaagct tgcttttagc ttttccataa
    12181 gttccacata attaatgttc tctttatcca ttttgttgat aaaaagaatt cggggcatat
    12241 tgcgttgttg ggtatagttc caagcttttt ctgttccgac ctcaattcct gaggaagcgt
    12301 caataacgat aacagctcct ccactaatac tgagggctcc tatggtttct ccgacaaagt
    12361 caaagtatcc tggagtatct aggaagttaa ttttcccttc agcccattcc atagggataa
    12421 tgcttgtacc tattgtaaac tgtctttgaa tctcttcctt gctgtagtca cctacagatg
    12481 ttccatcatc cacagaaccc aaacggtctg tgactttttt atcaaagagt atggcttcca
    12541 ataagctagt ttttcctgaa ccaccatggc ccagaagggt tacattgcgt attttgtcgc
    12601 tggcatattg tttcatcaat tcctcctgaa aaatgtatgt tttaattata gcactctttt
    12661 gatgatatac aaagaataat aagttactag ttttccttta gttaggggct taatggtgga
    12721 atcggcactg ttttcaaaaa aataaaggca ccggggtgcc tttattaaaa ttattcttat
    12781 cttattggac agacgcctgt ctcacagcct tcatctccaa tgtcgaataa tatctcctca
    12841 acctcgtatt tactaattaa agaaggtcta aaaggattca tttcggcctt acgcttttcg
    12901 tattcttcct tgctgatgct ctcatagggc aaaagatcat agaagttatc gtctaaggct
    12961 aagaagctta ttgctacgat atcatcccac ttttcccaaa cccattcttc tactgcctcc
    13021 cattcatgat cacgaacatg aacggtaata gaagcgttat gatctacgta gttttccata
    13081 aaaagtttat agttttcaag ttgttctatt gcaccaacat cgtactttgt tcttccctct
    13141 ggagccttta tgggaaattc tactaccttt gttgtgcagc tatcccattc ttggcccact
    13201 tctggatgga caggataccc tagttcctca caaactttaa caagagggtc tgttgcattg
    13261 attcgcactc ttcttaggaa ataaggagaa tgagagtagt gaatcccact gcttacttct
    13321 ggtaactggc taagggttcc ttctggtttt attgttgtaa ctagaagggg ggcttcaaca
    13381 tcaagttccg ctgcatagga ctcagcctca ctgcgagcta cactgcgaag ttttctaaga
    13441 aaatccgctt cttcttcttg tgtaagatta caggcattta ccatatcctg ccagccggtt
    13501 aaagaggtac ctattaaatt gtccctatgg tggacatggt tccagtggga tagttcaagc
    13561 tcgacctggg tcattctaaa ggctgctctg gcagaaagtt tttgtgcctt taaaagtcct
    13621 tgcatatcaa ggcttccatc ttccctagca aaggctgcga cattaaccgt tgtaagatta
    13681 cacaggcctt tattatcaag gagaatttct gcacagggat taactccttc aaagtttgga
    13741 cgccttttct ttcctgcttc ggcattaacc cagcctggtt cgcctgaata gcgcatctgt
    13801 tccatctgcc agctaaacat ctctctacta ggcttagagg tatagtagat gctgttattg
    13861 ctcatagagc ggtgaaggag ttctttatcc acaatccact gaccgtctat ctgtttgtag
    13921 agtttgcttt ttgcctcaat agactcacgg tcttcctgat caagtaagac tatttcagcc
    13981 gttctacgca caccaccaac cacaacattt tccccaatga tatttgctat atcaaggcaa
    14041 tcaatggggc gtaatttaat tctttgttca tcacttataa ggccagcttt ttttaccacg
    14101 cgatcaattt tgaggaacat gttttttagt gaactatgtc cact
//
LOCUS       DASRNA010000027        14086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_826973,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000027 DASRNA010000000
VERSION     DASRNA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826973"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFD08_00830"
     CDS             <1..937
                     /locus_tag="VFD08_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZK09587.1"
                     /translation="RKRKAYAIDPSDLGNFFHKIMERAMLDYSQGLFDQDQISSHIRK
                     IAKELLEEEEQYEAFLTGGAGKYLLERSLMTLESLLAYIISHSETSQFKPQFFEQSFM
                     IEEEKYTFRGIIDRVDILEDHFVAIDYKTGGKSFDLNKVFQGIDLQLMLYIDGFRAKK
                     PDLYPAGVFYFRLHDPLGDEKKEAQSFLKYDGLFIGEESLAQAYDPDIGRGLLPLKIT
                     KKGDYSKSSKVLDPEQAEKLLERSRNFARDYVEDILKGSMEILPLLDPRGGPGFTRAC
                     DSCDFAAICRFDPQAPGGDYSKFEELSDREIREVL"
     gene            938..4084
                     /locus_tag="VFD08_00835"
     CDS             938..4084
                     /locus_tag="VFD08_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZK09588.1"
                     /translation="MKWTDQQKEVIEARGVDILVSAAAGSGKTAVLTQRIIALIEEGY
                     SLDDFLVITFTRAGAKEMKEKIRRQLLKKSKESGKAFFEEEVKKLPRAQISTIHSFCS
                     AILKENFHLLDIDPNYRVAAEGERRLMEEESLDEVMEAYYKKEDPIFYELLEIFTNQR
                     SDQGLREVIIQIETFLSQKPRRQVYLDKMKDYYQEEDFWWELLASQTRQRLEEFESIY
                     NKTLPLIMTEDLLEFMEEERAMLYNLEKTGLSFKRFPTKKFMKEEANRVEIKAARDEW
                     KKLFENSLAKKDFKELYKKVQHSFLYIEKLIEISESFHQNFFARRLQENKLSFSDLEH
                     LALKAMEKEEVAQFYKEKFSFVFIDEYQDTNELQEYLVSLFVREGGLFMVGDVKQSIY
                     GFREARPDLFIEKYHSYNQLKKKRRIDLTKNFRSARPILEASNDLFQKLMREDFGGLE
                     YDDKARLVFGNKSLGDIEDQVEFLVTETPGDPHEEEIKSIISRIHQLIEEGYNYRDIV
                     ILYRSPKSFIEPAINLFRQAGLPLFSDQGESHLESLEVQILLNYLEVINNGFLDLPLL
                     SLLRLPRYAFTDQELYDLRGKERYFHQGFYDYMVEGELLEKKKFFLEEIKKLRWKSRQ
                     MSVAELLHYLYQEVEFEEFALLMSGGEQRLMNIRFLFERAKEFEETTMVGLPAFLTYI
                     DKLREQKQDRESAKLLGEDADVIRLMSIHKSKGLEFPLVFVAGLWKNYNEMSYRGPLF
                     FDDDVFVMDFFDLEERRKERSPFKDILIEKSKIKARQEEIRLLYVAMTRAEKKLILST
                     YAKEEIVKGARLNTWELKRQKSFHDLLVKSTPEFKPPAPKYVMMKNIAYDYIDSPGPR
                     GVEVASFNPPPPRKEAYKTSITSLISREEKTPSFELLGAEGGKERGTLFHKAMEFIDL
                     DLAQKDLDLALKRLEELGLLKDFSDAHLVEVFLDSSLGKRVVMSPKILRERPFILKKD
                     KRLVQGVVDLAFWEDGWVLVDYKTDRSFHYLDNYQRQLGLYKEAFEKITKEKVKEAFI
                     YFLRLNKAIEIKEKIDES"
     gene            4074..6002
                     /gene="tkt"
                     /locus_tag="VFD08_00840"
     CDS             4074..6002
                     /gene="tkt"
                     /locus_tag="VFD08_00840"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZK09589.1"
                     /translation="MNLENMATQTIRYLAVDAVNKAKSGHPGLPMGAAPMAKVLFRDH
                     LQFNPQDPDWLGRDRFVLSAGHGSALLYALLHLYGYDLSMEDLKAFRQVGSKTPGHPE
                     YRDTPGVETTTGPLGQGLANAVGMAMASKKMASQYGALFDNYTYVIVGDGDLMEGITS
                     EASSLAGHLKLGKLIVLYDSNNITIDGRTDITFSEDVRARYKAYGWECFLIADGNDEE
                     EISRVIEEAKKSDRPTFIEIKTIIGYGSPNKSDSSAAHGAPLGEEETALTKKATGWDF
                     KEDFFVPDQVRVYFEETVDKKKEAYAAWMKSFKEQKISFPSIKEGALEALMAEASGDK
                     ATRIHGEAMINTLNDFMPNLFGGSADLAGSNKTSLKAEEDFSSENPLGKNIHFGIREH
                     AMAAIVNGIAIHGAYRGYGATFLSFADYMKPSLRLAALMEIPTVSIFTHDSIGVGEDG
                     PTHQPIEQALMLRSIPGMEVYRPADGKETALAYYQAFSGEKPASILLTRQTLKELDIE
                     RKDAHRGGYIALREEKTPELILLASGSELSLALEAAKALRGEIDIRVVSMLNMEGFDG
                     QEEAYKKMVLPEEVRKRFAIEAASSLSWFKYIGLDGDYLCLDQFGASGPGDELFTLYG
                     FTLDNLIKEIKRYMNNGS"
     gene            5992..7371
                     /locus_tag="VFD08_00845"
     CDS             5992..7371
                     /locus_tag="VFD08_00845"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HZK09590.1"
                     /translation="MGVRITETILRDAHQSLIATRMPLEDMAPVLEQLDAVGYHALEM
                     WGGATFDACLRFLDEDPWERLRFIRRHVKKTKLQMLLRGQNILGYKHYADEVVEEFIE
                     RSVKNGIDIIRIFDALNDFDNIRLAMEATKKAGAHAQAAISYTTSPVHTREKFAEMAK
                     ELRDMGADSLCIKDMSGILLPYEGAELVKAIKAEVDLPLELHSHMTSGIADLMYLKGI
                     EAGADIIDTALSPLAMGSSQPATESFVMTLKDTPYETGLNIDILMELAKYFRIKKEEY
                     LEKGLLNPRVLGVDIRTLWYQVPGGMLSNLLSQLSESGRLDQFDLVMDEVPRVREDFG
                     YPPLVTPTSQIVGTQALFNVLTGERYKMVPEEVKAYMRGEYGQSPAPVNQELKDKILT
                     GQVPEAEKTKSLADFKKEVGEYYTQPEDVLSYALFPQVAKEFFKKRQARFLELDGDLL
                     DRKNKVYPV"
     gene            7368..7721
                     /gene="acpS"
                     /locus_tag="VFD08_00850"
     CDS             7368..7721
                     /gene="acpS"
                     /locus_tag="VFD08_00850"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZK09591.1"
                     /translation="MMVGIDLVFIPRIAALLKKGPFLQRYFTDSEIAYFQKSENPNVL
                     AGNFAVKEAFSKALGTGIRGFSLKDVEVLRDDLGKPVIYLHGSLARRKFRFLECSISH
                     DGDYATALVVMEEEL"
     gene            7718..8788
                     /gene="alr"
                     /locus_tag="VFD08_00855"
     CDS             7718..8788
                     /gene="alr"
                     /locus_tag="VFD08_00855"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZK09592.1"
                     /translation="MRPTQAKIHMYRLKENMELLREKSGQRTFLAVIKADAYGHGALR
                     CLDYLYKQGIGHFGVATFGEALELRAANEDIFIMVLGATDPSDYEEAARKKIRLPIFD
                     LASLEHYISLKEKGPIHIKIDTGMHRLGFAPEEFKKHIEKIKKINPEGIYTHIARADE
                     EDTSSAYGQIKRFNELVDYCDGQGLAFEFIHYANSATILHLDLGSTNMVRAGIALYGL
                     APDGKRSSGLRPVMSLHSKILSIREIPAGQGVSYGHTFIASRPSRIGTMPLGYADGYK
                     RQMSRRARVYAAGSYLPQVGNITMDQTMVDLTGTNVMLYDEVELMGEHISCEELALAA
                     GTINYEIVTTLGKRVDREYIYE"
     gene            8781..9173
                     /locus_tag="VFD08_00860"
     CDS             8781..9173
                     /locus_tag="VFD08_00860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZK09593.1"
                     /translation="MSKIHRQKKFKLKIQRGDIYLADLNPGVGSEQQGTRPVLVLQNN
                     IGNRFSPTIIVAPITGHIRRGNIPTHVLITEEISGLEKDSNVLLEQIRTIDKQRLITK
                     LGKLSKVALMDIEKATRISLGLDKKEEI"
     gene            9314..9991
                     /gene="ftsE"
                     /locus_tag="VFD08_00865"
     CDS             9314..9991
                     /gene="ftsE"
                     /locus_tag="VFD08_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZK09594.1"
                     /translation="MLELKEVSMRYPTGNIGLGDVSIKIDKGEFVFLIGASGAGKSTF
                     IKLLTKELSPTSGQLWFEDKNITKMHKRRIPHYRRKLGIVFQDYRLLEKETVAENVAF
                     AMRIIGKKNREIQRTLPLILNLVGLKGKEKIFPDQLSGGEQQRVSIARAIANNPKLLI
                     CDEPTGNLDPATSWELMDLLSKLHTHGTTVIMATHSNDIVDAMQKRVIELRDGELFRD
                     EQQGGYI"
     gene            9991..10878
                     /gene="ftsX"
                     /locus_tag="VFD08_00870"
     CDS             9991..10878
                     /gene="ftsX"
                     /locus_tag="VFD08_00870"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZK09595.1"
                     /translation="MNALSLLSSLKNIKRNALMSFASVFSITSTLVILGIVISLILNV
                     NHLTDEFVDKFNILNLQISDSYSDEQIDGLLASVKAEENIKSVVLITKEEALEKMKQD
                     FEGHSETLEGLPENPMPNVLRIELEDVEVAEETIIKLEEIDKDNEIHYYRDTVNKILK
                     ITDVIKTVGMVLILILLITTVFVVNNTIRVSIAGRRTEIGIMRYVGATRGFIRRPYII
                     EGVILGLLGAILSTIIVSYGYEEIMRMTLESLSQMSQNKPLLDTLYVVKRVGILAVVI
                     GIGVGMIGSIFSVRKYLRV"
     gene            10938..12134
                     /locus_tag="VFD08_00875"
     CDS             10938..12134
                     /locus_tag="VFD08_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZK09596.1"
                     /translation="MGFSFGANLEDLQKQVEEKEANVAEKEMQLEEIEAEAGSVEQQY
                     WNAVAEVEILQERIAETTEEIDLKDKAIIKTRAEVEEATEEYNFQAGEFGGRLRKMYL
                     TKDDSFWSMIFSAEGFEDLLMRISNYQRIVEMDEKTMDELNKQKVALEKLEEQLSNEM
                     EELQVLKEQQEVDKVLLDQKAVELEKRKAELEAMAAEISAQISAEAAAGAEIQAQMGY
                     LIEQARLQREAEAAAIRAAEEAARQEQENNTGDTSQGTTTDAPSRGDYVGGSWQWPTP
                     GYYYVSYGFGNRIHPVYGTSDYHNGIDILGDAGASIVAARDGLVVYAGWYSGYGNTVI
                     IDHGDGMQSLYAHGTGVGTSTGSYVYAGNYIMPMGSTGISSANHLHFGVMSGGAWVNP
                     SAYVGG"
     gene            12136..13383
                     /locus_tag="VFD08_00880"
     CDS             12136..13383
                     /locus_tag="VFD08_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZK09597.1"
                     /translation="MDRRRQISTILLTAIITVIAVIFVLKIVPFKIGDQVLIDYQEYE
                     KLQGLNRRFEKVLSLERTIKEEFYKDTAEIDFETGMVRGLFEALGDKYSVYFDKDEFS
                     QFKLELQGSFSGVGINIEPMVDGQIRVLSIIEDTPASASGILAGDRIFSVDDKPITED
                     ESHNAAVGRIKGPEGTEVVLGVYRYNSQTEEDEELNFTMKRAKIPLPLVESEMLGEGI
                     GYLRILGFDEGVHKKFLEAKKSLEDSGMEALVLDLRSNPGGSLNEAIKIADEILGEQI
                     IVSTQGPSFPKKVYESDAKKSLDIPFVVLMDNYSASASEILVGAIQDGEVAPVFGSHS
                     FGKGLVQDVISLGDGSGFKLTTNYYLTPKGRLITPDEPLLPDVSEEEILEKGFESTFD
                     EFGRYIDDGVLDYAVDYLNNIID"
     gene            13669..>14086
                     /locus_tag="VFD08_00885"
     CDS             13669..>14086
                     /locus_tag="VFD08_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZK09598.1"
                     /translation="MHTKKIYEYGPLLIFGAFLFLFVNNVGYIKEIFRITKPILWGIA
                     ISYVMNPICKYLMEKTKFRWSLNILIGYILLIISMGLFLWGLAPIIGQNISDFLRSIP
                     AIIKTIQQTLIDIERDLSQGPLAPLMAQLDLQSFINS"
BASE COUNT         4386 a         2504 c         3346 g         3850 t
ORIGIN      
        1 aagaaaaaga aaggcctacg cgattgatcc ttcggatttg ggaaattttt tccacaaaat
       61 tatggaaaga gccatgctgg actatagtca ggggcttttt gaccaagatc agatatcttc
      121 ccatataaga aagattgcaa aggagctcct tgaagaagaa gagcagtatg aagcctttct
      181 tactggaggg gccggcaaat atttactgga gcgcagcctt atgaccctag agagtctttt
      241 ggcctatatt attagtcata gtgagacaag tcagtttaag ccacagtttt ttgagcagag
      301 ctttatgata gaagaagaaa agtatacctt ccgcggcatt attgataggg tggatatctt
      361 agaggaccat tttgtggcca ttgattataa aaccggagga aagagttttg atttgaataa
      421 ggtcttccaa ggaatcgacc tccagctaat gctctatatt gatggattta gggctaaaaa
      481 gccagacctt tatccagccg gggtctttta ttttagactc catgatcctc taggagatga
      541 aaaaaaagaa gcccagagct ttttaaagta tgatggtctt tttattggag aagaatcctt
      601 ggcccaggcc tatgatccag atattggcag gggccttctt cccctgaaga ttacaaaaaa
      661 aggagactat agcaagtcca gcaaggtgct agatcctgag caagcagaaa agctccttga
      721 aagaagcaga aactttgcta gggactatgt agaagacatt ctaaaaggat ctatggagat
      781 cttgcccctt cttgatccta ggggcgggcc aggttttact agagcctgtg atagttgcga
      841 ctttgctgct atttgccgtt ttgatcccca ggctccaggg ggagactata gtaaattcga
      901 agaattaagt gatagagaga ttagggaggt tctataaatg aaatggaccg atcagcaaaa
      961 agaagtcatc gaggccaggg gagtagatat tcttgtttca gcagccgccg gttcaggcaa
     1021 aacagctgtt cttacccaaa ggattattgc ccttatagaa gaaggttact ccttggatga
     1081 ttttttagtc atcaccttta cccgggctgg ggccaaggag atgaaagaaa aaatacgcag
     1141 gcagctccta aaaaaaagca aggaatctgg caaggctttt tttgaagagg aggtaaaaaa
     1201 gctgccccgg gcacaaatat ctaccatcca ttctttttgt agtgctattt taaaggaaaa
     1261 cttccatctt cttgatattg atcccaatta ccgagtagca gctgagggag agcgtcgctt
     1321 gatggaggag gagagcctgg atgaagtcat ggaggcttac tataaaaaag aggaccctat
     1381 cttttatgag ctactggaaa tatttaccaa ccagcgtagt gaccaaggcc tgcgagaggt
     1441 gattattcaa atagagacct ttctttctca aaaacccaga agacaagttt atcttgataa
     1501 gatgaaagat tattaccaag aggaagactt ttggtgggag ctgcttgcta gtcaaacaag
     1561 gcagagacta gaagagtttg agagcatcta taataaaacc cttcctttaa ttatgacaga
     1621 agacttgcta gaatttatgg aagaagaaag ggccatgctt tataatttag aaaagacggg
     1681 tctttctttt aagcgctttc ccacaaaaaa gtttatgaag gaagaagcca atagggtaga
     1741 aattaaagca gcaagggatg agtggaaaaa actcttcgaa aatagccttg ccaaaaaaga
     1801 ctttaaagag ctctataaaa aagtgcagca ttcttttctt tatatagaaa aactaataga
     1861 gattagtgaa agctttcatc agaacttttt tgcccgtcgc ctgcaagaaa acaaacttag
     1921 tttttctgat ttagagcatc ttgcccttaa ggccatggaa aaagaagagg tggcccagtt
     1981 ttataaggaa aagttttcct ttgtatttat cgatgagtac caggacacca atgagctgca
     2041 agaatatctg gtttctttat ttgttcgaga gggcgggctt tttatggtgg gagatgtgaa
     2101 gcaaagtatt tatggctttc gtgaggccag gccagatctt tttattgaaa agtatcatag
     2161 ctataaccag cttaaaaaaa agcgccgcat tgatcttacc aagaactttc gcagcgctcg
     2221 gcctatcctt gaggcttcta atgatctttt ccaaaaactt atgcgagaag atttcggtgg
     2281 ccttgaatat gatgataagg caagactggt ttttggaaat aagagcctag gggacataga
     2341 ggaccaggtg gagtttcttg taacagaaac tccgggagat ccccatgagg aagaaattaa
     2401 gagcattatt tctcgtatac accagctgat cgaggaggga tataactata gggatattgt
     2461 gattctttat agatcgccaa aaagctttat tgagcctgct atcaatcttt ttcgccaggc
     2521 aggcctgccg ctttttagcg atcaaggaga aagtcacctt gaaagcctag aggtgcaaat
     2581 tctactaaac tatctagagg tcatcaataa tggttttctt gaccttcccc tgctgagttt
     2641 actgcgcctt cctagatatg cttttacaga ccaggagctt tatgatctga ggggaaaaga
     2701 aagatatttc caccagggct tttatgatta tatggtagaa ggagagctct tagaaaagaa
     2761 gaagttcttc ttagaggaaa tcaaaaagct gcgctggaaa agcaggcaaa tgagcgtggc
     2821 tgaactcctg cactatctct accaagaggt tgaatttgag gagtttgccc ttcttatgag
     2881 cggtggcgag caaaggctta tgaatattcg ctttcttttt gaacgggcca aggagtttga
     2941 agaaacaacc atggtggggc tgccggcctt tttaacctat attgataaac ttcgtgagca
     3001 aaaacaggac cgtgaaagtg caaaacttct tggagaagat gccgatgtca tccgcctaat
     3061 gagtatccac aaatcaaagg ggctagaatt tcctcttgtt tttgtggcgg gtctttggaa
     3121 aaactacaat gaaatgagct atcggggccc cttgtttttt gatgatgatg tctttgtaat
     3181 ggactttttt gatttagaag aaagaagaaa agaaaggtct ccctttaagg atatcctaat
     3241 agaaaaaagc aagatcaaag caagacaaga agagattcgc cttctttatg tagcaatgac
     3301 ccgggcggag aaaaaactga ttctttctac ctatgcaaaa gaagagatag taaagggggc
     3361 taggctaaat acctgggagc ttaagcggca aaaaagcttt catgatttgc tggttaaaag
     3421 cactcccgaa tttaagcccc cggctcccaa atatgttatg atgaaaaata tagcctatga
     3481 ttatatagac agtccaggcc cccggggggt agaggttgct agctttaatc ccccgccccc
     3541 tcgaaaggag gcctacaaga ccagtattac atctcttatt agtagagaag aaaaaacacc
     3601 cagctttgaa ttactaggcg cagagggggg aaaagaaaga ggcacacttt ttcataaggc
     3661 catggaattt atagatcttg atctagccca aaaagaccta gaccttgctc taaaacgact
     3721 agaggaactg ggactgttaa aagatttttc agatgcccat ctagtagagg tgtttttaga
     3781 ctctagcttg ggtaaaagag tggtaatgag tccaaagatt ttaagagaaa ggccctttat
     3841 cttaaaaaaa gacaaaagac tggtccaggg tgtggtggat ttggcctttt gggaggatgg
     3901 ctgggtactg gttgattata aaacagaccg ctcttttcat tacctagata actatcaaag
     3961 acaactgggc ctatataagg aagcttttga gaaaataaca aaagaaaaag taaaagaagc
     4021 ttttatttac tttttacgtt taaataaggc aatcgaaata aaggagaaaa ttgatgaatc
     4081 ttgaaaatat ggctacacaa acaattcgct atctagccgt tgatgcggtt aacaaggcaa
     4141 aatctggtca tccaggtctt cctatggggg ctgctcccat ggctaaggtt ctgtttaggg
     4201 atcatttgca atttaacccc caggatcctg actggttggg aagagaccga tttgtcctct
     4261 cagctggcca tggttctgcc ctgctttatg cccttttgca tctttatggc tatgatctga
     4321 gcatggaaga tcttaaggcc tttaggcagg tgggaagtaa aacgccaggt catccagagt
     4381 atagagacac tcctggggta gaaacaacca caggtcccct aggtcaagga ctggccaatg
     4441 cggtgggtat ggcgatggcc tcaaagaaaa tggccagcca atatggggca ctttttgata
     4501 attacaccta tgttattgtg ggtgatggag atttaatgga aggaatcact tctgaagcat
     4561 cttccctagc aggtcatcta aagctaggaa agctgattgt gctttatgat tccaacaata
     4621 taaccattga tggacgaacg gatattactt tttcagaaga tgtcagggcc cgctataagg
     4681 cctatggctg ggaatgcttc ttgatcgctg atggaaatga tgaagaagaa attagtcgag
     4741 taattgaaga ggctaaaaaa agcgaccgac caaccttcat tgaaataaag accataatcg
     4801 gttatggcag tccaaataaa agtgattctt ccgcagccca cggggccccc ctgggagagg
     4861 aagaaacagc cctaaccaaa aaagcaacgg gttgggactt taaagaagac ttttttgttc
     4921 ctgatcaggt cagggtctac tttgaagaaa cggtagataa aaagaaagag gcctatgcag
     4981 cttggatgaa aagctttaaa gagcaaaaga tttcttttcc ttctataaag gaaggagcat
     5041 tagaggccct tatggctgag gccagcggag ataaggccac ccggatccat ggagaggcca
     5101 tgataaatac cttaaatgac tttatgccaa atctttttgg tggttcagca gatcttgctg
     5161 gcagtaataa aacaagccta aaggcagagg aagatttttc ttccgaaaac cccctgggta
     5221 aaaatataca ctttgggatt cgagaacatg ccatggcggc gattgtcaat ggaattgcca
     5281 ttcacggcgc ctatagagga tatggagcaa cttttttaag ttttgcagat tatatgaaac
     5341 cctccctccg cctagctgct ctaatggaga ttccaaccgt ctccatcttt acccatgact
     5401 ccattggggt gggcgaggat ggaccgaccc atcagcctat tgagcaggct ctaatgcttc
     5461 gcagcattcc tggcatggag gtataccgcc cggcggatgg aaaagagaca gcccttgcct
     5521 actatcaagc ctttagtggg gaaaaaccgg cctctattct tcttaccaga caaacactaa
     5581 aagaacttga tattgagcgg aaagatgccc atagaggtgg ctatatagcc ctaagagaag
     5641 aaaagactcc agagcttatc ttactagctt cgggtagtga attgtctctt gccctagaag
     5701 cagctaaggc cctgcgggga gaaattgata tccgcgtggt tagtatgcta aacatggagg
     5761 gctttgatgg ccaagaagag gcttataaaa agatggtcct tccagaagaa gtaagaaaac
     5821 gatttgctat agaggcagct agctccctaa gctggtttaa gtatattggt cttgatggag
     5881 actatctctg cctggatcaa tttggagcca gcgggccagg agatgagcta tttacacttt
     5941 atggctttac cctagacaac ctaatcaaag aaataaagag gtatatgaat aatgggagtt
     6001 aggatcacag aaacaattct tcgtgatgcc caccagtcct taattgcaac caggatgccc
     6061 ctagaagata tggcgcctgt acttgagcag cttgatgctg ttggctacca cgccctggaa
     6121 atgtggggcg gggccacctt tgatgcctgc cttcggttct tggatgaaga tccttgggag
     6181 agattacgct ttattcgaag gcatgtaaaa aagaccaagc ttcaaatgct ccttcgtgga
     6241 caaaatattt taggctataa gcactatgca gatgaagttg tggaagaatt tatagagcgt
     6301 agtgttaaaa atggcattga tattattaga attttcgatg ccctaaatga ctttgacaat
     6361 attcgacttg ctatggaggc aacaaaaaaa gcgggtgccc atgcccaggc agctatcagc
     6421 tatacaacaa gtccagttca tacaagagaa aagtttgctg aaatggcaaa ggaactccgt
     6481 gatatgggag cagactccct ttgtattaag gatatgagtg gcattctgct tccctatgag
     6541 ggagccgagc tagtaaaggc cataaaggct gaggtggacc ttccccttga actccacagt
     6601 catatgacaa gtggtattgc tgatcttatg tatcttaagg ggattgaagc gggggctgat
     6661 ataatagata cagcccttag ccccctagcc atgggaagca gccagccggc tacggagtca
     6721 tttgttatga cccttaaaga caccccctat gaaaccggcc ttaatataga tattcttatg
     6781 gaactggcaa agtacttccg aataaaaaaa gaagagtatt tggaaaaggg acttcttaat
     6841 cccagggttt tgggggtgga tattcgtacc ctttggtacc aggttcccgg aggtatgctc
     6901 agtaatcttt tgagccagct ttctgaatct ggtagactgg atcaatttga cctagttatg
     6961 gatgaagtcc ccagggtaag agaagacttt ggatatcccc ctctggttac tcctacgtct
     7021 caaattgtgg ggacccaagc tctttttaat gtccttacgg gagaacgcta taagatggtg
     7081 cctgaagaag tcaaggccta tatgcggggg gagtatggac aaagtcctgc cccggttaat
     7141 caggagctga aagataaaat cctaactggc caggtcccgg aagccgaaaa aacaaaaagc
     7201 ttggcagact ttaaaaagga agttggcgag tattatactc agcccgaaga tgtgctaagc
     7261 tatgctctat ttccccaggt ggccaaggag ttttttaaaa aaagacaagc tagattcctt
     7321 gaactagatg gtgacttact tgataggaag aataaggttt acccggtatg atggtgggga
     7381 ttgatcttgt ttttatacca aggatagcgg cccttttgaa aaaggggccc tttcttcaaa
     7441 gatatttcac agatagtgag attgcttatt ttcagaagag tgaaaacccc aatgtcctag
     7501 caggaaactt cgctgtaaag gaagccttta gcaaggcctt aggtacaggg attcggggtt
     7561 tttctttaaa ggatgtagag gtgcttcgag atgacttggg caagcctgtg atttatctgc
     7621 atggaagctt ggcaaggaga aaatttcgtt ttctagagtg cagcatctct catgatggtg
     7681 attatgccac tgccctggtg gtgatggagg aggaattatg agaccaacac aagccaagat
     7741 acatatgtat cgcttaaaag aaaatatgga gctactaagg gaaaaatctg gccagagaac
     7801 cttccttgct gtcattaaag ctgacgccta tggtcatggc gctcttagat gtttagacta
     7861 tctttataag caagggatag gtcattttgg ggttgctacc tttggggagg ccttggaact
     7921 tcgcgctgcc aatgaggata tttttattat ggtcttgggt gccactgatc cctcggacta
     7981 tgaagaggct gccagaaaaa aaatacggct acctattttt gatctagcca gtttggagca
     8041 ctatatcagt ctaaaagaaa aaggacctat acatattaag attgatacgg gtatgcaccg
     8101 actgggtttt gcgccggagg aatttaaaaa acatattgaa aaaataaaaa aaataaatcc
     8161 ggaagggatt tatacacata ttgccagggc cgatgaagag gatacttcct cagcctatgg
     8221 ccagattaaa cgctttaatg aactggtgga ctattgtgat ggccagggac tggcctttga
     8281 atttatccat tatgcaaact cagcaacgat tttacatctt gatctgggtt ctacaaatat
     8341 ggttagggca ggaatagccc tctatggcct agctcctgat gggaaaagaa gttctggtct
     8401 tagaccggta atgagtcttc acagcaagat tttatccatt cgagaaatcc cagcaggcca
     8461 gggagtaagc tatggccata cctttattgc ctcaaggccc tcgcgcattg ggactatgcc
     8521 cctaggctat gctgatggct ataagcgcca gatgagtcga agggcccgag tatatgcggc
     8581 gggtagctat ctacctcaag taggcaatat taccatggac cagaccatgg tagatcttac
     8641 tggtacaaat gttatgcttt atgatgaagt ggagcttatg ggtgaacata tttcctgcga
     8701 agagctagcc ctagctgcag ggacaatcaa ttatgaaata gtgaccacct taggtaagcg
     8761 ggtggacagg gagtatattt atgagtaaaa tacatagaca gaaaaaattt aaacttaaaa
     8821 tccaaagggg agatatttat cttgccgatc ttaatcctgg tgtaggaagt gagcagcagg
     8881 gaaccaggcc tgtgctagta ctgcaaaaca atattggcaa ccgatttagt cctactatta
     8941 ttgttgcccc tattaccggt catatccgaa gaggcaatat cccaacccat gtacttatta
     9001 cagaagaaat ttctggtctt gaaaaagatt ccaatgtgct cttagagcag attcgaacaa
     9061 tagataaaca acgtcttatt actaaattgg ggaaactttc taaggttgcc ctaatggata
     9121 ttgaaaaagc gacaagaatc agtttgggtc tggataaaaa agaagaaatt tagtaagaaa
     9181 atagacggct taatcctaac aaggatgtaa taagtgtact ttttcgggga aaaagcgtgt
     9241 ttttttacca atatacttgc atattttccc ataagaggct tataatagtg agcaccttga
     9301 aaaggagaac atcatgttag aacttaaaga agtatctatg cgttatccca cgggcaatat
     9361 tggcctaggg gatgtaagca taaaaattga taagggagaa tttgtttttc taataggagc
     9421 tagtggtgct ggaaaaagca catttattaa gcttttgacg aaggaacttt cccccacctc
     9481 aggccagtta tggtttgagg ataagaacat caccaagatg cataaaagaa gaataccgca
     9541 ttatcgccga aaactaggta tcgtttttca agattatcgt cttcttgaaa aggaaactgt
     9601 ggcagaaaat gtggcttttg ccatgcgtat tattggaaag aaaaaccgcg aaattcaaag
     9661 aaccttgcct cttattttaa atctagtggg attaaaagga aaagaaaaga tctttcccga
     9721 ccaactttct ggaggggagc aacaaagggt tagtattgca agagcaatcg ctaacaatcc
     9781 aaagcttctt atctgtgatg agccaacggg aaatcttgac ccagcaacat cctgggaact
     9841 tatggactta ctgagtaagc ttcataccca cggtacaaca gttataatgg caacccatag
     9901 caatgatatt gtagatgcca tgcaaaaaag agtaatagaa ctgcgggatg gggaactttt
     9961 ccgtgatgag cagcaagggg gctatattta atgaatgcct taagtcttct ttcttcgcta
    10021 aaaaacatta agcgcaatgc attaatgagt tttgcatcgg tcttttctat tacatctact
    10081 ttagttattt tggggattgt aatttctctt attcttaacg tcaatcactt gaccgacgag
    10141 tttgtagata aattcaatat tttaaatctt caaattagtg atagctatag tgatgagcag
    10201 attgatggtc ttcttgctag cgttaaagca gaagaaaata ttaaaagtgt cgtcttaatc
    10261 accaaggaag aggcactaga aaaaatgaaa caagattttg aaggacatag tgaaacatta
    10321 gaaggattgc ccgaaaaccc tatgcccaat gtacttcgaa ttgaacttga ggatgtagaa
    10381 gtagcagaag agacgattat aaaactggaa gaaattgaca aagacaatga aatccattac
    10441 tatagagata ccgttaataa gattttaaaa attacagatg taataaagac agttggtatg
    10501 gtcttgattt taattttatt aattacaacg gtcttcgttg tcaacaatac cattcgtgta
    10561 agtattgctg gaagaagaac tgagatagga attatgcgct atgtcggtgc gaccagagga
    10621 tttattcgaa gaccctatat aattgaaggt gtaattctag gacttctagg tgccatacta
    10681 agtacgatta ttgttagcta tggttacgag gagattatga ggatgacact agagagctta
    10741 agccaaatga gccaaaacaa gccgctactg gacacactat atgtagttaa aagagtgggc
    10801 attttggcag ttgttatcgg aattggtgtg ggtatgatag ggagtatctt ctcggtacgg
    10861 aagtacttac gagtatagag gtgaaaatta tgaggaagtt tattgcattt ttgttggtgt
    10921 ttacaatgac actctcgatg gggttctcat ttggagcgaa tctggaagac ttacaaaagc
    10981 aagtcgaaga aaaagaagcg aatgtggcag aaaaagagat gcaactagaa gaaatcgaag
    11041 ccgaagctgg ttctgtagaa caacagtact ggaatgctgt agcggaagtt gaaattcttc
    11101 aagaacggat tgcagaaacg acagaagaaa tcgatttaaa ggataaggcc attattaaga
    11161 ctcgagctga agtcgaagaa gccacggaag aatataattt ccaagcagga gagtttggag
    11221 gacgtcttag aaaaatgtac ttaaccaagg acgactcttt ttggagtatg atctttagtg
    11281 cagaaggatt cgaagactta cttatgagga tttcgaatta tcaacgcatc gttgagatgg
    11341 atgaaaaaac gatggatgaa ttaaacaaac aaaaagtggc tcttgagaag ttagaagaac
    11401 agctcagtaa tgagatggaa gaactccaag tacttaagga acaacaagaa gtcgacaagg
    11461 ttcttttgga tcaaaaagca gttgagttag aaaaacgcaa ggccgaacta gaagccatgg
    11521 cagcagagat atcggcgcag atttctgccg aagcagctgc gggtgcggaa attcaagcac
    11581 agatgggtta tttaatagag caagcacgct tgcaaagaga ggccgaagca gctgctataa
    11641 gagctgctga agaagccgct aggcaagaac aagaaaataa tacgggcgat actagtcagg
    11701 gcactaccac tgacgctcct agtcggggtg actatgtagg gggtagctgg cagtggccga
    11761 ctccgggata ctattatgta tcctatgggt ttggtaaccg catacatcct gtatatggta
    11821 cttcagacta tcacaatgga atcgacattc taggtgatgc gggtgcttca attgtagcag
    11881 cgcgtgatgg tcttgtagtt tacgctggtt ggtattctgg ctatggtaat actgttatta
    11941 ttgaccatgg cgatggaatg caatctctat acgcccacgg gactggtgtt ggaacatcta
    12001 ctggtagcta tgtctatgct ggaaactata ttatgcccat gggaagtacg gggatttcct
    12061 ctgctaacca tctccacttt ggtgttatga gtgggggagc ttgggttaat ccttcggctt
    12121 atgtaggggg ctaggatgga cagacgtcgg caaatttcta caatattact cactgcaatc
    12181 attacggtga ttgcagtgat ttttgtttta aaaattgtcc cctttaagat aggggatcaa
    12241 gtcttgattg attatcagga atatgaaaaa cttcaagggc taaatcgtcg ctttgagaaa
    12301 gtgcttagtc ttgaacgaac cataaaagaa gaattttata aagacacagc agaaattgat
    12361 tttgagacag gtatggttcg agggcttttt gaagctctag gggataagta ctctgtttat
    12421 tttgataaag atgaattttc ccagtttaag ctagagttac aaggcagctt ttctggagta
    12481 ggtataaata ttgagcccat ggtggatggg cagattagag ttttatctat tatcgaagat
    12541 acgccggcat cggcttctgg aatcttggca ggagatcgta tatttagtgt tgatgataaa
    12601 cctatcaccg aggatgagtc ccataatgcg gcagtagggc gaatcaaggg gcctgagggc
    12661 acagaggtcg tcctgggtgt ctatcgttat aatagccaga ctgaggaaga tgaagagctc
    12721 aattttacta tgaaaagagc aaaaatcccg ctaccacttg ttgaaagtga aatgctaggt
    12781 gaggggattg gttatcttag aattttaggt tttgatgaag gagttcataa aaaattccta
    12841 gaagctaaaa agtctcttga agatagcggt atggaagccc ttgttttaga ccttcgtagc
    12901 aatcctggcg gaagtctaaa tgaggccatt aaaatagcgg atgaaatcct aggagaacag
    12961 attatcgttt ctacccaggg gccgagtttt cctaagaagg tctatgaatc tgatgccaaa
    13021 aaatccttgg atattccctt tgtggtactt atggataatt acagcgcctc agcctcagag
    13081 atacttgttg gcgctattca agacggggaa gtggcaccag tttttggcag tcatagcttt
    13141 ggtaagggtc ttgtgcaaga tgttattagt ttaggtgatg gttctggttt taagttaacg
    13201 accaattatt atctaacacc taagggcaga ttaattacac cagatgaacc ccttcttcct
    13261 gatgtaagcg aagaagagat ccttgaaaaa ggattcgaaa gtaccttcga tgaatttgga
    13321 cgttatatag acgatggggt gcttgattat gcggtggact atttaaataa tattattgac
    13381 tgatatcagt taaaggggca ggaaaaactg cttctttttt ttgccaaaaa tggccatcaa
    13441 ttggcagggg taggcctatc aattgtcgat tgagattgtt ttaataatga taaggaaaaa
    13501 gagcatttct atctttagat ggtatataaa gagcttttaa agtagacaag caagtccatg
    13561 tagagctaaa taagaacttg tcgtatgttt tgaagtgttt tttgatatat atccttcaaa
    13621 aagatgaaat ataagtaaag atatagtata ataacaaagg aggtagtgat gcacactaaa
    13681 aaaatatatg aatatggccc actgcttatt tttggcgcgt ttttattttt atttgttaat
    13741 aatgtaggct atattaaaga gatctttcgg attaccaaac cgattttatg gggtattgcc
    13801 attagctatg ttatgaatcc tatttgcaaa tatcttatgg aaaaaactaa gtttcgatgg
    13861 tctttgaata ttcttatagg atatattttg cttattatat cgatgggtct gtttttatgg
    13921 gggctggctc ctattatagg gcaaaatatt tctgatttct taagaagtat tcccgcaata
    13981 ataaaaacaa ttcagcaaac actgatcgat attgaaagag atttatccca gggtcccttg
    14041 gctccactaa tggcccagct agacttacag agttttataa acagta
//
LOCUS       DASRNA010000028         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_678481,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000028 DASRNA010000000
VERSION     DASRNA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678481"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1357
                     /gene="dnaJ"
                     /locus_tag="VFD08_00890"
     CDS             209..1357
                     /gene="dnaJ"
                     /locus_tag="VFD08_00890"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZK09599.1"
                     /translation="MSKRDYYEILGVERDADDQAIKSAYRKLAMKYHPDKNPGDQEAE
                     ATFKEINEAYEILSDTSKRQKYDQFGHAGVDPNASAGYGDFGGFGGFEDVSDIFSSFF
                     GGGSFGGFDEFNQGASRGPRRGSDVGADVHLSFREAYTGASKEVSFYRLESCPTCDGS
                     GAAEGSSVSTCSNCQGAGQIKEVQAGLFGQNVRIRTCPECHGTGQKPDKACPTCKGKG
                     KVRKRRKIRVKIPAGVDTGHVMTLSGEGDIGEDGGPKGDLQLRIFVASDPVFTRKGVD
                     VFQEIPISFTQAALGNEIQIEGLDGKVKLKVDAGTQPGTVRRISGGGFPSVRGYGKGD
                     HYVTFVIKTPKDLNETQKEALRNFAQTMGEDYTKENKGFFGKVKDAIK"
     gene            1344..>2254
                     /locus_tag="VFD08_00895"
     CDS             1344..>2254
                     /locus_tag="VFD08_00895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZK09600.1"
                     /translation="MPLNRSSHRGLFSLEEDLEYLIIKVKSKNAEKLEEELSFYKIDG
                     LEILNPQDRMYYESSWQEDEAPDQPLGEEAVLKIYGEKKELQNIVHSYRDIILLHSYE
                     KNEDKDWNALWALQFKGIKQGDIFVRPPWIDKKENYIDLIIEPGMGFGTGSHETTLLS
                     LGALRDYLKPKAYVYDVGTGSGILAILAKKLEASFVHAIEIDEMALKNARVNAELNNL
                     DIYFSQGDLLSNCQYRADLIVANILPPILKRMKDDAYNLLNPGGILLLSGILDKRVEE
                     VLEVYGRDFILKENRSMGQWHVLILEK"
BASE COUNT          742 a          372 c          548 g          592 t
ORIGIN      
        1 gatacactag atgagataac agaagcgata aaagaacttg aagccgcaat acaacctttg
       61 gctcaaaaac tctatgaaca agctgccgcc gaagcttctg ctgaaggcga agcacctagt
      121 agtgatgatg tcgaagaagc agattacgaa gttgtagact aagattatgg aatagacttt
      181 taagtctatt ccttttatga gggatagaat gagcaaacga gactattatg aaatattagg
      241 cgttgaacgt gatgccgacg atcaggcaat aaaaagtgcc tatcgtaaat tggccatgaa
      301 atatcatcca gataaaaatc ctggagacca agaggccgaa gcgactttta aagagatcaa
      361 tgaagcctat gaaatactaa gtgatacgag taaaaggcaa aagtatgatc aattcggtca
      421 tgcaggtgtt gatcccaacg cttcagcagg ctatggagat tttggcggtt ttggcggatt
      481 tgaagacgtc agtgatatct tttccagttt ttttggagga ggtagttttg gcggatttga
      541 tgagttcaac cagggcgcat cccgtggtcc aagaagaggc agtgatgttg gggctgatgt
      601 acatctaagc ttccgtgaag cctatacagg tgccagtaaa gaagttagct tctatcggct
      661 ggaatcctgt cctacttgtg atggaagcgg agctgctgaa ggttcttcgg tctcgacttg
      721 cagcaattgt cagggcgcgg ggcaaataaa agaagtccaa gcaggacttt ttggccaaaa
      781 tgttcgtata agaacctgtc ctgaatgtca tggaacaggt caaaagcctg ataaagcttg
      841 ccctacatgt aagggcaagg gaaaagtacg aaaaagaaga aaaatacgag taaaaatccc
      901 tgctggagtt gatacgggtc atgttatgac cttatcaggt gagggagata taggagaaga
      961 cggcggtcca aagggagatc ttcagcttag aatttttgta gcatcagatc cagttttcac
     1021 tcgaaaggga gtggatgttt ttcaagaaat ccccatcagc tttacccaag ctgcccttgg
     1081 taatgaaatt caaatagaag gacttgatgg aaaagtcaag cttaaggtag atgctggtac
     1141 ccaacccgga acagtaaggc gcataagcgg tggaggcttt cctagtgtaa ggggttatgg
     1201 taagggtgat cattatgtaa cctttgtgat aaaaaccccc aaagatctca atgaaactca
     1261 aaaggaagca ctaagaaatt tcgcccaaac tatgggggaa gattatacaa aagaaaataa
     1321 aggctttttt gggaaggtaa aggatgccat taaataggag ctcccatagg gggctctttt
     1381 ctttggagga agacttggag tatttaatta ttaaggtaaa aagcaaaaat gcagaaaaat
     1441 tagaagaaga actttccttt tacaaaatcg atggactaga aattctaaac ccccaagata
     1501 ggatgtatta tgagtctagc tggcaagaag atgaggcccc agatcagccc ttgggagagg
     1561 aagcagtttt aaaaatttat ggagaaaaaa aagaactaca aaatatagtc cattcttacc
     1621 gtgatattat cctcctgcat tcttatgaaa aaaacgaaga taaggattgg aatgcgcttt
     1681 gggcccttca gtttaagggt attaagcagg gagatatctt tgtaagacct ccttggattg
     1741 ataagaaaga gaactatata gaccttatta tagagccagg gatgggattt ggtacgggaa
     1801 gccatgaaac gaccctgctg tctcttggag ccttaagaga ttatctaaaa cccaaagcct
     1861 atgtctatga tgtgggcaca ggaagtggaa ttttagccat actggccaaa aaactagagg
     1921 caagttttgt tcatgcaata gaaatagatg aaatggctct taaaaatgcc agagtaaatg
     1981 ctgaactaaa taacctagat atttatttct cccaggggga tttacttagt aattgtcaat
     2041 atagggcaga tcttattgtt gcgaatattc tgccccctat tttaaaacgc atgaaggatg
     2101 atgcctataa tcttcttaat cctggaggta ttctattgct ttcgggaata cttgataaaa
     2161 gagttgaaga agttttagag gtttatggca gggattttat tttaaaagaa aatcgttcca
     2221 tgggccagtg gcatgtatta attttggaga agat
//
LOCUS       DASRNA010000029         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_605544,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000029 DASRNA010000000
VERSION     DASRNA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605544"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFD08_00900"
     CDS             <1..276
                     /locus_tag="VFD08_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZK09601.1"
                     /translation="SRKSIIAAIVLALGRGMGETMAVMMVIGNAPIFPKLFNKTQTIA
                     SLIALEMGMAEVGSMHYHALFASGFVLMIMLLLINLALYFIKARIDA"
     gene            295..1131
                     /gene="pstA"
                     /locus_tag="VFD08_00905"
     CDS             295..1131
                     /gene="pstA"
                     /locus_tag="VFD08_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZK09602.1"
                     /translation="MSEKTRDYILKIWISLSVLLVLSLLVYIFGYIFKSGLAAIDFDF
                     IFSGPKGRPIGSEGGIFPAIVGSVFLMLIACIFAAIFALSTSIYIVFYSPSKKVEKMI
                     HLIIQSMAGIPSIVLGLFGYSFLVLGLGLGRSLLAGGLTLSIMIFPYMQIRIEKTFRE
                     VEEDLIESSYALGASKSFTIFRLILPLCKSDIVSAITLAGGYAMGASAPIIATAAVLF
                     APAPRSLLSPVMALPFHLYILTGEGISLEKAYGTALVLIVLLLILNVFSLALNLKRRE
                     DL"
     gene            1132..1875
                     /locus_tag="VFD08_00910"
     CDS             1132..1875
                     /locus_tag="VFD08_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HZK09603.1"
                     /translation="MDILRINNLSAKYTNKRVLRDLSLGIKKNKITAIIGPSGCGKST
                     LLIVLNRMIEEKGGSFTGEILFKDKDILDYGKEEIRKNIGMVFQNPTPFPFSIYKNLT
                     YAPIYYGIKNRAELDRIVESSLKKAGLFDEIKGDLTMPATKLSGGQQQRLCIARALTS
                     RPEILLLDEPCSALDVKNTQNIEKLLLGFSKDYTIIIVTHNLAQAKRIADYTAFILDG
                     ELVEYGQTRELFKNPKDARTKEYIEGIYG"
     gene            1954..2856
                     /locus_tag="VFD08_00915"
     CDS             1954..2856
                     /locus_tag="VFD08_00915"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HZK09604.1"
                     /translation="MEFKQLECFVAVARRKSFSKAGDVLLLSQPAVTSNIQNLEKEFD
                     VLLFDRNKEVTLTKAGKKFYPYALEMLNIRDKSMMAIDQHKNNIEGVLEICTSTIPEQ
                     HLLPHIIKAFKKIYPKVKFSLRHKDSQEVVEEILSGSSNFGFVGAKYFHKGIKYIDFF
                     EDRLVVIASAKKTFTSEVIGIEDLLGEEILLREEGSGTRKLIENALKEKGLDLDLFSS
                     QIISHSLEAIKNMVALNIGISFASEVSVKDQVASGRIKQYEVKGLNLNRHFSLAYSEA
                     RYLFPVEEKFKEFVSEWRWDRADL"
BASE COUNT         1013 a          519 c          669 g          963 t
ORIGIN      
        1 agcagaaaaa gtattatagc agccattgtt ttggctctag gaagaggtat gggggaaaca
       61 atggcggtga tgatggttat aggaaatgcc cccatatttc ctaaattatt taataaaacc
      121 cagactatag cctcgctaat agccctggaa atgggtatgg cagaggtggg aagcatgcat
      181 tatcatgccc tttttgcctc tggttttgtg cttatgatta tgctacttct aatcaatcta
      241 gccttgtatt ttataaaagc taggatagac gcgtaaattt aggtaggaaa aaaaatgagt
      301 gaaaaaaccc gtgattatat acttaagatt tggattagcc taagtgtact attagtgctt
      361 tctcttctag tttatatatt tggatatatt tttaaaagcg gacttgctgc tatagatttt
      421 gactttatat tttccggccc caagggaaga cccattggtt cagagggtgg gattttccct
      481 gctattgtgg gaagtgtatt tcttatgctt atagcttgta tattcgctgc tatctttgcc
      541 ctttcaacat cgatatatat tgtcttttac tctccctcta aaaaagtaga gaaaatgata
      601 cacttaatta ttcaatccat ggcagggatt ccttctattg tactaggatt atttggctat
      661 agctttttgg tactcggttt gggtcttggt aggtccttgt tagctggagg gctgactctg
      721 tctataatga tttttcccta tatgcagatt agaatagaaa aaacatttag ggaggtggaa
      781 gaggatctaa tcgaatcctc ctatgcttta ggagcgtcta aatcttttac catatttaga
      841 ctgatccttc ccctatgtaa atctgatata gtttctgcta tcactcttgc tgggggctat
      901 gctatggggg cctctgcccc tattatagca acggcggcgg tactatttgc tcctgcccca
      961 aggtccttac tttctcctgt aatggccctt ccctttcatt tgtatatttt gacgggggaa
     1021 gggatatctc tagaaaaagc ctatggtaca gctttagtgc ttatagtact actacttatc
     1081 ttaaatgttt tttcactggc cctaaatttg aaaagaaggg aggacctcta gatggacatc
     1141 cttagaataa ataatttatc tgcaaaatat acaaataaaa gagtgctaag ggatttgtct
     1201 ttaggcataa agaaaaataa gataacagct ataattggac catctggctg tggcaagtca
     1261 acccttctta ttgttttaaa tcgcatgata gaagaaaaag gcgggagttt tacaggagaa
     1321 atactattta aagataaaga catactagat tatggcaagg aagaaatcag aaaaaacata
     1381 ggtatggtct ttcaaaaccc cacccccttt cccttttcta tctataaaaa cttaacctat
     1441 gctccaatct attatgggat caaaaacagg gccgagttag ataggatagt agaatctagc
     1501 cttaaaaaag cgggtctttt tgatgaaata aagggggatt taaccatgcc agccaccaag
     1561 ttatctggtg ggcagcaaca aagactctgc attgccaggg cgctgacttc aaggccggaa
     1621 atcctcttac tagatgagcc atgttctgcc ttggatgtaa agaataccca aaatatagaa
     1681 aagctgcttc taggattttc taaagattat actataatta tagtgaccca taatttagcc
     1741 caggcaaaaa gaatagcaga ttatactgct tttatcttgg atggtgagtt agtcgaatac
     1801 ggacaaacaa gggagctatt taaaaatcca aaggacgcta ggacaaaaga atatattgaa
     1861 ggaatatatg gatagggtcc aaaacctcag gactagggta taataaattc caaggaagaa
     1921 aatattggaa taaatcgaca ggagttggga aaaatggagt ttaaacaatt ggaatgtttt
     1981 gttgctgttg ctcgaagaaa gagcttctct aaggcaggag atgtattact tctttctcag
     2041 ccagctgtaa caagtaatat tcaaaactta gaaaaagaat ttgatgtgct cttatttgat
     2101 agaaataagg aggttaccct aaccaaggca ggcaagaagt tttatcccta tgcccttgag
     2161 atgcttaata tccgggataa atctatgatg gcaattgatc aacacaaaaa caatatcgaa
     2221 ggggtgttag aaatatgcac tagtacaata ccagagcagc atctgctacc tcatattatt
     2281 aaggccttta aaaaaatcta tcctaaggtt aaattctccc ttcgtcacaa agattcccag
     2341 gaagtagtgg aggaaattct ctcagggagt agtaattttg gatttgtggg ggccaagtat
     2401 ttccataaag gaataaaata catagatttt tttgaagata gattggttgt aattgcttct
     2461 gctaaaaaaa cctttactag tgaagttatt ggtattgaag atttactagg agaagaaatt
     2521 ctccttaggg aagaaggctc ggggacaaga aaattaattg aaaatgcctt aaaagaaaaa
     2581 ggacttgatt tagatctctt ttcttctcaa atcattagcc atagcttgga agcaataaaa
     2641 aacatggtgg ccctaaatat tggcattagt tttgcatcgg aagtttcagt aaaagatcaa
     2701 gtagcctcag gcagaataaa gcaatatgaa gtcaaaggcc ttaatcttaa taggcatttt
     2761 agcctggcct atagtgaggc aaggtatctt tttcctgtag aagaaaagtt taaggaattt
     2821 gtaagcgagt ggagatggga tagggcagac ctttaggagt aggggtggga gcatatttat
     2881 tcccacttat gcaaaaagga taaaacaagg ctaatagata ctagactacc caagagaaga
     2941 aagctagata ttatgttgat aggaaaccct ccactagaga caagtcccat atctataggt
     3001 aagggtgggc taatgcagtt tgaattagct cgttttttta tatttgaaag tcttattcta
     3061 atggggatta cttgtctaca gatttttttc cgagtgctag tgcccgagaa tgtagacctt
     3121 tacgtcttgg tgtgaaaaaa cgttgaaggc aagaagtatt ttta
//
LOCUS       DASRNA010000030         6586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_827631,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000030 DASRNA010000000
VERSION     DASRNA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827631"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFD08_00920"
     CDS             <1..515
                     /locus_tag="VFD08_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HZK09605.1"
                     /translation="LKNIILGTYFYGISGTFLSFIVFGNYGLSQQLAGKIDLIGTVAQ
                     GTDINLGIIEIFSTLPLTNFLLIALFVMMVTFYTTTFDSITLVVSAYSYKNLPPGEEP
                     SRLVRGFWATMFIVFPIALIFAENSINSLQSISIIAAFPIGLVFILIIVSFFKDAKAY
                     LKEMPISDRN"
     gene            complement(642..1823)
                     /locus_tag="VFD08_00925"
     CDS             complement(642..1823)
                     /locus_tag="VFD08_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZK09606.1"
                     /translation="MTDLIKEVKNIEEQMISWRRDLHRIPEIGLDLPKTSDYIRRALE
                     DLGIEYKELLNGNALVGLIRGQEEGRTIALRADMDGLPVREETGLDFASKDGRMHACG
                     HDGHMAMLLGAAKILQDKRHLLKGNVKLLFQPAEESPGGALPMIEEGALENPKVDAII
                     GLHNGNLHKDIPKGKIGVRFGPMMASADRVFITIKGKGAHGAYPEQSIDPITIAAELI
                     LALQTIISRETAAVDPAVLSICKIEGGYNTNIIPDEVKLEGTVRSFNRETRKMLARRI
                     EEVTRGITATMGAECQIKYEFSYPALINSRSFTEFFIDSAKKIIPESDIVILTEPVMA
                     AEDMAYLLEKVPGTFFFLNNIGEIEGLAYPHHNAKFDLDEGLFYKGTALLVQTVLDYL
                     D"
     gene            complement(1928..3481)
                     /locus_tag="VFD08_00930"
     CDS             complement(1928..3481)
                     /locus_tag="VFD08_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZK09607.1"
                     /translation="MAYSPPLGSSITNTRMRTPDYLSPYSGMCAVCASNCSGLCEIGL
                     SAVRGSEAIYPYMTDINQFASEKDYPLDFSHFNINGRVFGARGCKEDAFMATFAKADI
                     TCSFGLENKVPLKAPFVLPAMAKLNWQDYYAGAALAGVLVVIGEDVIAKDDELLLEDG
                     KLVSSPLIEEMLGEFRKYDTGYGDIILQANYDDESYGVLDYAISKLGVKSVELKFGQG
                     SKGIQGMGRVESLEKAQAHHKRGYLVYPDPNDPEIAENYEKGMGQVFEKIGKLPIWNE
                     EILVKRVKELKSLGAQRVCFKVGPFDPEDLLEILKIASKAEVDLITFDGAGGGSGNSP
                     TKMMNEWGMPTVYMESVLYKMLKAMKEKGYALPQIAVAGGFVMEDQVYKGLALGAPYI
                     DLIGIGRGAMSAAMSSKIIGERIKENKIPKSYEKYGKTLEEIFSGYRELKPTYGEKTA
                     SISPGAVGVYSYLNRVEIGLKQLMALNRKFALKYISREDLVPLTSLAAEVSGLDTFND
                     KTEKLLEKL"
     gene            complement(3663..5192)
                     /gene="hutH"
                     /locus_tag="VFD08_00935"
     CDS             complement(3663..5192)
                     /gene="hutH"
                     /locus_tag="VFD08_00935"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZK09608.1"
                     /translation="MNYLEIDGDHLDLESFIDVTRRGKKVRLSGQAEKKVQKARDFVD
                     DLIKENKVVYGLTTGFGKFSDVLIPENETKALQRNLIVSHACGLGNPLEEDLVRGVML
                     LRINALAKGNSGIRLSTLQTLLAMLNKGVHPIIPEKGSLGASGDLAPLAHMVLTMMGE
                     GQAVYKGEQIKAKEAMARAGIPLIELLSKEGLALINGTQVMTSIGAHTLYDAINLSKT
                     ADIAAGLSSEALGAITDAFDPKVHSIRAHPGQIDTAKNILRILEGSKMTTKQGELRVQ
                     DAYSIRCIPQVHGASKDTLNYVKEKVEIEMNAATDNPLIFVDEKEVISGGNFHGQPMA
                     LPFDFLALGLAELANISERRLERLVNPALSEGLPAFLTEEGGLNSGFMIVQYAAAALV
                     SENKILSHPASVDSIPSSANQEDHVSMGTIGARKAREIMENVRKVLAMEILAACQAID
                     LRGKKDLGKGSAVAYKLLRQKISKVEEDRIMHVDIQGCEELLKENKIVEALEQAIGRL
                     S"
     gene            complement(5292..>6586)
                     /locus_tag="VFD08_00940"
     CDS             complement(5292..>6586)
                     /locus_tag="VFD08_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZK09609.1"
                     /translation="LRGHLFVSSGLGGMSGAQGKAAKIAGGVGIIAEVDPSRIKTRLE
                     QGWVDQSFKDLEALFKAAFLAMKEKKATALAYEGNIVDLLEYALEKEIHIELLSDQTS
                     CHAVYEGGYCPQKMSFSERTKMLLEDKKAFKERVDQSLLYHYQLIKKLVDKGTYFFDY
                     GNSFLKAIYDAGETSIAQNGINDKDGFIFPSYVEDIMGPMLFDYGYGPFRWVCLSGKK
                     EDLIKTDKAAMDGIDPNRRGQDRDNYNWIRDAQKNDLVVGTQARILYQDAQGRLKIAL
                     KFNEMVRNGEVGPIMLGRDHHDVSGTDSPFRETANIKDGSNIMADMATQCFAGNAARG
                     MSLVALHNGGGVGIGKAINGGFGMVLDGSERVDEVLKTAMPWDVMGGVARRAWARNDH
                     SIETAIIYNKKRKETDHLTLPFLADSELVRKLVEEKTK"
BASE COUNT         1666 a         1571 c         1213 g         2136 t
ORIGIN      
        1 cattaaaaaa tattatttta ggaacctatt tctatgggat ttctgggacc ttcctatctt
       61 ttattgtctt tggtaactat ggactctcac aacaacttgc tggcaagata gatcttatag
      121 gcactgttgc ccaggggacg gatattaatc ttggtatcat tgagattttt tctactttac
      181 ccctaacaaa ctttttgctt attgctcttt ttgttatgat ggtcaccttc tatacaacga
      241 cctttgactc cattacttta gtggtgtctg cttattctta taaaaatctg cctcctgggg
      301 aggaaccctc ccgtttagta agaggttttt gggctactat gtttattgtt tttcccattg
      361 cccttatctt tgcggaaaac tctatcaatt ctcttcaatc aatctccatc atagctgcct
      421 ttcccatagg acttgttttt atcctgatta ttgtgagctt ttttaaggat gctaaagctt
      481 atctaaaaga aatgcctatt tcagatagaa attgaaattt cttttctttg acaaaaaagg
      541 tttttccttt aagtagataa aaaatatcct tcctacttat ctgctagatg cagaaaaaag
      601 atgctgatta aataccagca tctttttatc ttcccccttt tttagtccaa ataatccaat
      661 accgtttgga ctaaaagggc cgttccctta taaaaaagac cttcatcaag atcaaatttt
      721 gcattgtgat ggggataagc cagtccctca atttcgccaa tattgtttaa aaagaaaaag
      781 gttccaggta ctttttctaa taaataagcc atatcttctg ctgccataac cggctctgta
      841 agaataacta tatcactctc tggaataatt tttttagcag agtcaataaa aaactcagta
      901 aaactacggg aattgattag ggctggatag gagaactcgt attttatctg acactctgcc
      961 cccatagtgg ctgtaattcc cctggtgacc tcttcaatac gcctagccag catttttctg
     1021 gtttccctat taaaggagcg aacggttcct tccagcttta cctcatctgg aataatattg
     1081 gtattataac cgccctctat cttacaaata gaaagaacag cagggtctac tgcagcagtc
     1141 tccctgctta taatggtttg aagggccaga ataagctctg ctgctatagt gattggatca
     1201 atagactgtt ctgggtaagc accatgagct ccctttcctt ttatggttat aaacacccta
     1261 tcagcggagg ccatcatggg cccaaagcgt acaccaattt tgcccttagg aatatccttg
     1321 tgaagatttc cattatgaag tcctataata gcgtcaacct ttgggttttc tagggcgcct
     1381 tcttctatca tgggcagggc cccaccaggg gattcttctg ctggttggaa aagtagtttt
     1441 acatttcctt taagtaggtg gcgcttgtcc tgaagaattt ttgcggcacc tagtagcatg
     1501 gccatatggc catcatggcc acaggcatgc atcctgccat ctttagaagc aaagtccaac
     1561 cctgtttctt ccctaactgg taagccatcc atatccgctc ttagggcaat ggttcttcct
     1621 tcttcttggc cgcgaataag tcctacaagg gcatttccat ttagaagttc tttatattca
     1681 atacccaagt cttctagggc ccttcttata tagtcagagg ttttaggtag atcaaggcct
     1741 atctctggta tcctatggag atctcttctc caggaaatca tctgctcctc tatgtttttt
     1801 acttctttta ttagatcagt catcttatac ctcgtttttt atttatttct actagtaaag
     1861 ctaagggatc ttcccagcta gcaagacccc ttagctcttt tttgattagg taaattagat
     1921 aaagcgttta gagcttttct aagagttttt ctgttttatc attaaaggtg tccagtccac
     1981 ttacctcagc agcaaggctg gtaagaggca caagatcttc ccggctaata tatttaaggg
     2041 caaattttcg gttaagggcc attaattgtt taagcccgat ttccactcgg ttcaaatagg
     2101 aatagacccc tactgctccg ggagaaatac tagctgtttt ttctccatag gtaggtttta
     2161 gctccctata acccgagaat atttcttcta gggtctttcc gtatttttca tagcttttag
     2221 ggattttgtt ttctttgatt ctctcaccga ttatcttaga agacatagca gcactcatag
     2281 cccctctgcc aatgccaatt aggtcaatat agggtgcccc cagggctagg cccttgtaga
     2341 cctggtcttc cataacaaag ccaccagcta ctgctatctg tggcagagca tagccttttt
     2401 ctttcatggc ctttagcatc ttgtagagca cactttccat ataaaccgta ggcatacccc
     2461 attcattcat catttttgta gggctatttc cagagccccc accagcccca tcaaaggtga
     2521 ttaaatccac ttcggctttt gaggctatct ttaggatttc tagtaaatct tcggggtcaa
     2581 agggacctac tttaaaacat accctctggg ctcctaggct tttaagctct ttgactcgct
     2641 ttactagaat ctcttcattc catatgggaa gttttcctat tttttcaaat acctgaccca
     2701 tccctttttc atagttttct gcgatttctg ggtcatttgg atcaggataa actaaataac
     2761 cccttttatg gtgggcctgg gctttttcta ggctctctac tcttcccatg ccctggatac
     2821 cctttgagcc ttgaccaaac ttaagttcaa cgcttttaac acccagctta cttatagcat
     2881 aatccaagac cccataagac tcatcatcat aattagcctg gagaataata tcaccatagc
     2941 ctgtgtcgta ttttctgaat tctcctagca tttcttcaat tagaggggag ctaaccagct
     3001 tcccatcttc aagaagaagc tcatcatcct tggcgattac atcttcgcct atcactacaa
     3061 gaaccccagc aagagctgcc ccagcgtagt agtcctgcca attcaacttt gccatagcag
     3121 gaaggacaaa gggggctttt aaaggcacct tgttttccag gccaaagcta caagtaatgt
     3181 cagccttggc aaaggtagcc ataaaagcgt cttccttaca gcccctggca ccaaaaactc
     3241 ttccattaat gttaaagtgt gaaaaatcca agggatagtc tttttctgaa gcaaactggt
     3301 tgatatcggt catataagga tagattgctt cactccctct tacagcagat aaaccaattt
     3361 cacacaaacc actacagttt gaggcacaca ctgcacacat gccagaatat ggtgatagat
     3421 aatcgggagt tctcattcta gtatttgtta tactacttcc taaaggtgga ctataagcca
     3481 tatttaacct ccttgttaaa cctttcttct tctttaattg taactcttat aaaaaacgaa
     3541 tgcaatttta atcaggaggg gggggctttt taggcgctcc ctcattattt cttctggagt
     3601 ttttcttttt tttactatag acaagcccta gaagctaggc ctgtctataa atttgcctag
     3661 tattagctta atctaccgat tgcctgctct agggcttcta ctatcttatt ttcttttaat
     3721 agctcttcac agccctggat atctacatgc ataattcggt cttcttctac ttttgatatt
     3781 ttctggcgaa gtagcttata ggccacagca cttcccttgc ccaagtcttt ttttcccctt
     3841 aggtcgattg cctgacaggc tgcaaggatc tccatagcaa gaactttacg aacattttcc
     3901 ataatctccc tggcctttct agctccaatg gttcccatgg aaacatggtc ctcttgattt
     3961 gctgaggagg gaattgaatc aacagaggct ggatgagaaa ggattttatt ctcagatacc
     4021 aaggcagcgg ctgcatactg tacaatcata aaacctgaat taaggcctcc ttcttctgtt
     4081 aaaaaagccg gaagtccttc actaagggca ggattgacca ggcgctccaa tcttctttct
     4141 gagatatttg ctagttctgc caggcccaga gctaaaaagt caaagggcag ggccatgggc
     4201 tgtccatgga agtttcctcc agaaatcact tctttttcat caacaaaaat aaggggatta
     4261 tccgttgcag cattcatttc tatttccacc ttttctttga cataatttaa ggtgtcctta
     4321 gaagccccat gaacctgggg aatgcagcga atagaatagg catcttgtac ccgaagctct
     4381 ccttgttttg ttgtcatctt acttccttca agaatacgca ggatgttttt tgctgtgtca
     4441 atttgccctg gatgagctcg aatggagtga accttggggt caaaggcgtc agtaatagcc
     4501 ccaagggcct ctgaactaag gccggcggct atatcagctg tcttgcttag attgattgca
     4561 tcatagaggg tatgggctcc aatggaagtc ataacctggg tcccattgat aagggccagt
     4621 ccttctttag ataggagctc aataagagga attcctgccc tggccatggc ttcttttgcc
     4681 tttatttgct cccccttata tactgcctgg ccttctccca tcatggtaag aaccatatgg
     4741 gctagggggg ccaagtcccc actggcgcca agggaccctt tttctggaat gattggatga
     4801 acccccttat ttaacattgc taagagagtc tggagggtgg atagacgaat gccagaattt
     4861 cccttagcta aggcattaat tcttagcagc ataacacccc gcaccaggtc ctcctccaag
     4921 ggattaccaa gtccacaggc gtggctaact atcaaatttc tttgcagggc cttggtttca
     4981 ttttctggta taagtacatc gctaaatttc ccaaaacctg ttgtcaagcc gtatactact
     5041 ttgttttctt tgattaagtc gtccacaaaa tctctggctt tttggacttt cttttctgct
     5101 tgaccactta gtcttacctt ttttcccctt cttgtaacat ctataaagct ctctagatcc
     5161 agatggtctc cgtcgatttc taaataattc atttgccctt ccttgtttac tttatttggt
     5221 taaattagcc gcaactcttc ctaggctcag ggtctaggtt tttacgtggc caaagactct
     5281 ttggccacta tttattttgt tttttcttcc actagttttc ttactagctc actatctgca
     5341 agaaagggaa gggttaaatg atcggtctct ttacgctttt tattatagat aatggccgtt
     5401 tctatcgagt ggtcatttct tgcccaggct cttctggcaa cgccccccat tacatcccag
     5461 ggcatggccg ttttaaggac ctcatctacc cgctcacttc catctaaaac catcccaaag
     5521 ccaccattaa tggcttttcc aattccaaca cccccgccat tatgaagggc aaccagactc
     5581 attcccctag cagcattacc agcaaagcac tgggttgcca tatccgccat aatattgctt
     5641 ccatccttaa tatttgctgt ttctctaaag gggctatcgg ttccagaaac gtcatgatgg
     5701 tctcttccga gcataatagg tccaacctca ccatttctga ccatttcatt aaactttagg
     5761 gcgatcttca gtctgccctg ggcgtcctga taaagaatcc tggcctgggt tccaaccaca
     5821 agatcgtttt tttgtgcatc gcgtatccag ttataattat ccctgtcctg tcccctgcga
     5881 tttggatcta tcccatccat ggctgcctta tcggttttaa taagatcttc cttttttcca
     5941 cttaggcaaa cccagcggaa aggaccatag ccataatcaa aaagcatggg gcccattata
     6001 tcctcaacat aggaggggaa aataaagcca tccttatcat ttattccatt ttgggctatg
     6061 ctggtttctc ccgcatcata gatggcctta aggaaactgt ttccatagtc aaaaaagtag
     6121 gttcccttgt ccactagttt tttgattaat tgatagtgat aaagaaggct ctgatccact
     6181 ctttctttaa aggccttttt atcttcaagg agcatttttg ttctttcact aaagctcatt
     6241 ttctgaggac aatagccccc ctcatagact gcgtgacagg aggtttggtc tgagagtagc
     6301 tcaatatgaa tttctttttc aagggcgtac tctaaaagat ctactatatt tccctcatag
     6361 gcaagggcgg ttgctttttt ctctttcatg gccaaaaaag ctgccttaaa aagagcctct
     6421 aggtctttaa aggactgatc cacccagcct tgctcaaggc gggtcttgat cctagaagga
     6481 tcaacctctg caatgatccc cactcctcct gcaatttttg ctgcttttcc ctgggcccca
     6541 ctcattcccc ccagccctga gcttacaaat aagtgacccc tcagat
//
LOCUS       DASRNA010000031         6159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_827767,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000031 DASRNA010000000
VERSION     DASRNA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827767"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1072
                     /locus_tag="VFD08_00945"
     CDS             95..1072
                     /locus_tag="VFD08_00945"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HZK09610.1"
                     /translation="MKTKVFISSVNDPRVNLALEEMLTQDCKEDEYIFYLWQNENTIV
                     IGRNQNPHRECDLQKVEEDGVILVRRPSGGGAVFHDLGNLNFTFIAHEKKYSQQMNFE
                     IVLDALKVFGLEGEFSGRNDLLIEDRKFSGNAYSHYKNTRVHHGTLMLEVDLTKMSRY
                     LNVHPLKMKAKGVTSVRSRVINLYELAPQMKVEDLKEALIGSFEARLGRAEKIEQLDK
                     ASLRGYKHADKYFSWEWNIGETPNFSLELENKFDWGLISFFLDLNKGRIKRAKIFTDA
                     LVNEDFAGLGAALEGSEFNKKSMEKVLFSQLSDKKISKELIEWLSSQGI"
     gene            1102..1473
                     /locus_tag="VFD08_00950"
     CDS             1102..1473
                     /locus_tag="VFD08_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09611.1"
                     /translation="MKTFIRKCKRLGMSPIERMIDHLRYGEFKKAGKVAKKGISNIEG
                     KELAQSVTDKLAFLDRGQREAFFDEFEKVSEEAEDILPDEAERAQAKNWCKHSLEKDD
                     LFLVLGLAVAFCFAIRRFRNR"
     gene            1577..2053
                     /locus_tag="VFD08_00955"
     CDS             1577..2053
                     /locus_tag="VFD08_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK09612.1"
                     /translation="MTTIESIATAYKNYIWKLSYSILQDSYLAEDATQETLMILAKNL
                     EKIDLTSPRSHGFITVVARHKAYEFYHKEKKYTLGNEEDLAAHIKEPLDHLGELMSAI
                     HSLSPLYSTVLLLSSVHGYSDSEIASLLDIAPATVRKRKERARNKLKEIYYGEEGQ"
     gene            2050..3072
                     /locus_tag="VFD08_00960"
     CDS             2050..3072
                     /locus_tag="VFD08_00960"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZK09613.1"
                     /translation="MSFEKDLKQALSDYEQSLVDYYNELPDPKRRKKMKGKRNWYIAF
                     ASLAAVLVLFVGLRGILPGMKSSQSGDLEMMEISSEGAFDHPAEATEEMAVEEEAQGA
                     LEGALTSDQLPEDEKIIHTYNLTIETKDIKTSIEKLESLIKKQGGFIENAQQNGQMQV
                     GENANAFYIFRIPREKSEALKGELEKLGQTQYFSQQQDNVTKSYRDTQARVDLLRTKE
                     DRLMELLEIAKDLEDLIAIESQIMDLQFEREQMQAYLNDLDQQVAYDTYYLDLVQVKV
                     FTEKSFTQKIKDSFTSGLEGFRNFLEGSLLFLIYNWAFILLFGLLILAFSLIFKAVRK
                     KKNKER"
     gene            complement(3097..4386)
                     /locus_tag="VFD08_00965"
     CDS             complement(3097..4386)
                     /locus_tag="VFD08_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HZK09614.1"
                     /translation="MIKIVFFDIDGTLFSEKTLRVPPTALYSLKELQKKGIKIIAATG
                     RGPSVAAFLEEETGLTFDGYISLNGQYCFTKNQVLRKQAIDKKELRLIRDYLEEKNFS
                     YDLFGKDFTLRNKRSKKAEFSDLLLGEKASSFTYGPLEDPSKEVYQIKAYMPKEEDPL
                     LKSFLQESRLLRWKSLYNAVFAKNGGKKRGVETFLEHYKIKKEDSLALGNGNNDLEFF
                     DAVAYKVAMEDSPDPLKKKATIITASPDNEGIYKALLELKLLPQAELWEALYEDESPA
                     GFYLARGVAIPKGYFHLVAEVLVSHTDGDYLLVRRAKEKRKLPGRWEASASGSALPGE
                     SSLEAAKREVQEETGIPRGNFRFLSKHTYPELGIIFHNYHCQTDVQKKSLRMQKGESE
                     EYRWVNKEDFLAFMESDKAILWQRERLSSYLEEIKNR"
     gene            complement(4383..5417)
                     /locus_tag="VFD08_00970"
     CDS             complement(4383..5417)
                     /locus_tag="VFD08_00970"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZK09615.1"
                     /translation="MIARTTRGKYTDLSYSGHIVVANAQGDILYSHGDPLRFSFARSS
                     TKPLQALLALETGALDHYKITDKELALMCGSHGGSTEHTKVVGEILDKGGLSQKDLQC
                     GAHPPLMKTARKELEKTGQEPNVLHNNCSAKHSAMLLSASYLGQDLKTYTQISHPHQQ
                     SIIKLLGEFTSMDPGDFGLAIDGCGVPVHGAPLYKWAQAFARLDETSSFSKSRQEHIL
                     RLSQAMAKYPEMVAGPARLCTDLLIHFKDRVIAKGGANAFYALSLKNQGLGIAIKVES
                     GESRLLAGIILQTLLQLGIISPEEMSPFESYTKQELINHQGVLVGETLVDFKLQKHPH
                     PSPIKKERIL"
     gene            complement(5392..5967)
                     /locus_tag="VFD08_00975"
     CDS             complement(5392..5967)
                     /locus_tag="VFD08_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZK09616.1"
                     /translation="MKTIILVLLLSSPFFYLTSLDLRVLEGLLAIRNPAFTLLAFLFA
                     FLGSWRFLVPFNLLFAFFFRKKKPFALLIPLGTLSCWVLNHLIKELIQRQRPPIVTLA
                     IENSFSFPSGHAMVSSCFILLLLIFIKKTWDKDFLVPAVIYIGLMALSRLYLGVHYPS
                     DVLVGSALGLAYAWALYQYFGGKYDRSNHKG"
BASE COUNT         1857 a         1166 c         1425 g         1711 t
ORIGIN      
        1 agatggacca tttgcgtgtt gagcgattgc gtcttaatat aatggatgaa aagtaaggcc
       61 ccagaccagg ggcctttcta tcgaaaggac ctttatgaaa acgaaagttt ttatttcatc
      121 ggtaaatgat cctcgggtaa atttagctct tgaagagatg ctgacccagg actgtaaaga
      181 agatgagtat atattttatc tttggcaaaa tgaaaatacc attgttattg gccgcaatca
      241 aaaccctcat agagagtgtg atcttcaaaa agttgaagaa gatggggtta ttttggtgcg
      301 ccggcccagt ggcggagggg cagtatttca tgatttgggg aatttgaatt ttacctttat
      361 cgcccatgaa aagaaatatt ctcagcaaat gaattttgag attgtcctag atgccctaaa
      421 agtctttgga ctggaaggag aattcagtgg acgaaatgac cttttgattg aagatcgaaa
      481 gttttctgga aatgcctata gccattacaa aaacacaagg gttcatcatg gaacccttat
      541 gctagaggta gatctgacta aaatgtcccg ctacctaaat gttcaccccc ttaaaatgaa
      601 agccaaggga gtcactagtg taagaagtag agtaataaac ctctatgagc tagcccccca
      661 aatgaaggta gaggatctta aagaagctct tattgggagc ttcgaggcaa ggctgggtag
      721 ggcagaaaaa attgagcagc ttgataaggc tagtcttaga ggctataagc atgcagataa
      781 atattttagc tgggaatgga atattgggga gacgcccaat ttttcccttg agctcgagaa
      841 taaatttgac tggggcctga tctctttttt tcttgattta aataagggcc ggattaaaag
      901 ggccaaaatc tttaccgatg ctcttgttaa tgaagatttt gctggtctgg gggccgccct
      961 tgaaggaagc gaatttaata agaaaagcat ggaaaaagtt cttttctctc aacttagcga
     1021 taaaaaaata agtaaagagc tgattgagtg gttgtcttcg cagggtatat aagaagtaga
     1081 caaagaaagg aagggacaaa tatgaaaact tttattagaa aatgtaagcg actagggatg
     1141 tctcctattg aacgcatgat tgatcacctt cgctatggag aatttaaaaa agcaggaaaa
     1201 gttgccaaaa agggaatttc taatattgaa ggaaaagaat tagcgcaaag tgttacagac
     1261 aaactagctt ttcttgaccg agggcagcgc gaggcttttt ttgatgaatt tgaaaaggtc
     1321 tcggaagagg cagaagatat tctaccagat gaggcagaaa gggcccaagc caaaaattgg
     1381 tgtaaacaca gcttagaaaa agacgatcta tttctagttt tgggtctcgc agtagccttt
     1441 tgctttgcga ttagacgatt tagaaacaga taatctaaaa gtcaaataag taaaaagtaa
     1501 aacaagggag tagattatct acttccttgt cacatttagc ccaatctatc cctttatagt
     1561 atagagaggt gggagagtga caacaataga aagtattgct acagcctata aaaattatat
     1621 atggaagctt tcatattcta tcctacaaga tagttatctt gctgaggacg ccacacaaga
     1681 aaccctaatg atcttggcta aaaatcttga gaaaattgac cttaccagtc ccagatctca
     1741 tggttttatt actgtggtgg ctaggcataa ggcctatgaa ttttatcata aagaaaagaa
     1801 atacaccttg ggaaatgaag aggacctggc agcgcatatt aaggagcctc ttgatcactt
     1861 aggtgaactt atgagtgcca ttcatagctt aagccccctc tatagcacgg ttcttttgct
     1921 aagctctgtc catggctata gtgatagcga gattgcaagt cttcttgata tcgctccggc
     1981 tactgtgcgc aagcgaaagg aaagagcacg caataaatta aaagagatct attatggaga
     2041 ggaggggcaa tgagtttcga aaaagattta aaacaggctc ttagtgatta tgaacagtcc
     2101 ctagtggact attataatga gctccctgat cctaaaagga gaaaaaagat gaagggaaaa
     2161 agaaactggt atatagcttt tgcctcgcta gcagctgtcc tggtgctttt tgttggatta
     2221 agaggaatac ttcctggaat gaagagttct cagagcggtg atttagagat gatggaaatt
     2281 tcttctgagg gagcctttga ccatcccgcg gaggcgacag aagaaatggc tgtggaagaa
     2341 gaagcccaag gcgcactcga aggggccctg accagcgacc agcttcctga agacgaaaag
     2401 attattcaca cttataatct tactatcgaa acaaaggata ttaaaacaag tattgaaaag
     2461 ctagaaagcc ttatcaaaaa acagggtggt tttatagaaa atgcccagca aaatgggcaa
     2521 atgcaagtgg gagaaaatgc caatgcattt tatatttttc gaatccctcg tgaaaaatcc
     2581 gaagccctaa agggagaact tgaaaaacta ggccaaacac agtatttttc acagcaacaa
     2641 gataatgtaa caaaaagcta tagggatacc caggcaagag tggatctttt aaggaccaag
     2701 gaagacagac taatggaact ccttgaaata gctaaagacc tggaagatct aattgccata
     2761 gaaagccaga ttatggatct gcagtttgaa agagagcaaa tgcaggccta tctaaatgac
     2821 cttgaccagc aagtggccta tgacacctac tatctggatt tggttcaagt aaaggtcttt
     2881 acagaaaaaa gctttactca gaaaataaag gatagcttta ctagtggcct tgaaggcttt
     2941 agaaactttc tagaaggcag cctgctattt cttatttaca actgggcctt tatccttctt
     3001 tttggtctgc tgatcctggc ctttagcctt atctttaagg cggtaagaaa aaagaaaaat
     3061 aaagagcgat aaaaaatagg taggcccctt agaggcctac ctatttttta tttcctcaag
     3121 ataagacgat agacgttctc tttgccaaag gatggcctta tcagactcca taaaggcaag
     3181 gaagtcttcc ttatttaccc agcgatattc ttcgctttct cctttttgca tacgaagact
     3241 ttttttctgg acatctgttt ggcagtgata attatgaaag ataattccta gctcaggata
     3301 ggtatgcttg gaaagaaaac gaaagttgcc cctagggatt cctgtttctt cctggacctc
     3361 ccttttggct gcttctagag agctttctcc cgggagagca cttccactag cagaagcttc
     3421 ccatcttccg ggcagttttc ttttttcttt tgcccggcga acaagaagat aatcaccatc
     3481 tgtatgagat accagcacct cggccactag atggaagtag ccttttggga tggctacacc
     3541 ccgggctaga taaaaaccag ccgggctctc atcttcataa agtgcctccc aaagctcggc
     3601 ctgggggagt aactttagct ctaatagggc cttatagatt ccttcattat caggacttgc
     3661 tgttataata gtagcttttt tctttagtgg gtcaggggag tcttccatgg ctaccttgta
     3721 agccactgca tcaaaaaact caaggtcatt atttccattt cccagggcca ggctgtcctc
     3781 tttttttatt ttatagtgct ccaaaaaggt ctctactccc cttttcttgc ccccgttttt
     3841 ggcaaaaact gcattgtaga ggcttttcca gcgaagaaga cggctttctt gcaagaagct
     3901 ttttagcaag gggtcttctt ctttgggcat ataggcctta atctgataga cctctttgga
     3961 tgggtcttct aaagggccat aggtaaagga tgaggccttt tcacccagca gaaggtcaga
     4021 aaactctgct ttttttgatc ttttatttcg cagggtaaaa tcttttccga atagatcata
     4081 gctaaagttt ttttcctcga gatagtctct aatgagtctt agctcttttt tatctatggc
     4141 ctgcttgcgc agtacttgat tttttgtaaa gcagtactgg ccattgaggc tgatatagcc
     4201 atcaaaagta aggccggttt cttcttctag aaaggcagcc acactgggac ctctgcctgt
     4261 ggcagcaatg atttttatgc cttttttttg cagctccttt aaagagtaaa gggcggtagg
     4321 tggcacccgc aaggttttct cactaaaaag agttccatct atatcgaaaa aaactatctt
     4381 tatcatagga tcctttcctt ttttataggg gagggatggg ggtgtttttg caatttaaag
     4441 tcaacaagag tttctcccac aagcactcct tgatggttta taagctcttg ctttgtataa
     4501 ctttcaaagg gactcatttc ttcaggggag attatgccca gttgaagaag ggtttgaagg
     4561 atgatgcctg ctagaaggcg ggactctccg ctttctactt ttatggcaat gccaagtcct
     4621 tggtttttta gacttagggc ataaaatgca ttggcacctc ccttggcaat caccctgtct
     4681 ttaaaatgaa ttaaaagatc agtacagagt ctagctgggc ctgcgaccat ctctggatat
     4741 ttggccatgg cctggctaag gcgaaggata tgctcttgcc tgcttttgct aaaggagctt
     4801 gtctcatcca ggcgggcaaa ggcctgggcc cacttataaa ggggggctcc atgaacggga
     4861 actccacagc catctatggc aaggccaaaa tctcctgggt ccattgaagt aaattcaccc
     4921 aagagtttga ttatgctctg ctggtgggga tgagaaattt gcgtataggt ctttaaatct
     4981 tggcccaaat aggaggcgct taggagcatg gcactgtgtt ttgccgaaca attattatga
     5041 aggacgttgg gctcttgacc ggttttttca agttcttttc gcgctgtttt cattaggggc
     5101 ggatgggctc cacactggag gtctttttgg ctaaggcctc ctttatccaa gatttcccct
     5161 actactttgg tatgttctgt actaccgccg tggctgccac acataagggc tagttcctta
     5221 tctgttattt tgtagtggtc tagggccccg gtttctaggg ccaaaagtgc ctgaaggggt
     5281 tttgttgaac ttcgagcaaa gctaaagcga agggggtctc catgggagta gagaatatcc
     5341 ccctgggcat ttgctaccac gatatggcca gagtagctaa ggtcggtata tttacccctt
     5401 gtggttcgag cgatcatatt ttcctccaaa atactggtag agtgcccagg cataggccag
     5461 tcccagagcg cttcctacaa gaacatcact ggggtagtgg acgcctaggt aaaggcggct
     5521 tagggccata aggccaatat aaataacggc agggactagg aagtccttgt cccatgtttt
     5581 tttaataaaa atcaaaagca gtagaataaa gcaactgcta accatggcgt ggccgctggg
     5641 aaaagaaaag ctattttcta tagcaagggt gacaataggt gggcgttggc gctggataag
     5701 ttctttgatc aaatgattta atacccagca gcttaaggtg cctaggggta tcaagagggc
     5761 aaagggtttt ttctttcgaa agaaaaaagc aaataaaaga ttaaagggaa caagaaagcg
     5821 ccaggagcca agaaaggcaa aaagaaaagc aagcagggta aaagctggat ttcttatggc
     5881 tagaagtccc tcaagaaccc ttaaatccaa agaagttaaa taaaaaaagg ggctacttaa
     5941 taaaagcact aaaattattg ttttcataat tcctccgcta ttagtttagc atataagggt
     6001 ataaatggag aaagaattag ggaggaaaag aggtagatat gaaaaaaagt gtcatgctta
     6061 tggggctatg ggctcaagag gcagaaaaaa caccagatga actagaaaaa gaggtccgcc
     6121 tacgtttaga ggaaataccc tctgcgggag atctttatc
//
LOCUS       DASRNA010000032         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_157389,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000032 DASRNA010000000
VERSION     DASRNA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157389"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VFD08_00980"
     CDS             <1..1614
                     /locus_tag="VFD08_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZK09617.1"
                     /translation="VINTIKKVVDKERLFDSIWTSLEELLKDYDKSEIKRINLSTTVS
                     TNAIVEDKTSPVGMIIQSGPGINNDFSSCKAQIEEITGYIDHRGVIVESLDLVEVQRA
                     LDAFKKQKISSCAVVSKFSTRNPKNELSIGKILDKDFGNISLGHRMSGKLNFPRRVYS
                     SYLNAAVYGTFKDFSTNIKRSLEKEGVKSPIYILKADGGTMDIKTAEKMPVETILSGP
                     AASFMGISAMLPTEEDAILLDVGGTTTDIFFLADGVPLFEPLGAQISDYKTLVRAIYS
                     VSIGLGGDSAVSVEDASLKIGPRREGRPFALGGPKPTPSDAMIYLGLMDVSEDLAKEK
                     AKEAMELLADKLNIRAKEVAERILTTMAEIIKKKTDQLLIKINSKPVYTIGELLHGKK
                     VKPKNLYLIGGPARVLSPLLEKQYDLPCHFPANFAVANAIGAALAKPTMEITMLADTS
                     QRILSVPELGVYEKIPKNYTLDRAKAKARELLKSYALSAGLKEEDFESEITEESSFNM
                     VDGFYTKGKNIRVKAQIKPGHILKLRSDN"
     gene            1616..2920
                     /locus_tag="VFD08_00985"
     CDS             1616..2920
                     /locus_tag="VFD08_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZK09618.1"
                     /translation="MKAKNKLGLVFFPAFDWAISPTHPEREERLLYTQDQIFEEGIED
                     IEGIEMFNPLIAQEKDIQRVHFCVPNVTSRLTESHFISVGGAIRAAQAVMNKEVDKSF
                     ALVRPPGHHAHRVVYGDRGFCVVNIEAIMLEKMRQEFGPLRVAIVDTDCHHGDGTEDI
                     YWNDKDTLFISFHQDGRTLYPGTGAIGDLGGPSAYGYNVNIPLPPRTGEEGFLYVLNK
                     VVKPILDDYKPDIIINSAGQDNHYSDPITNMNFTAQGYAKLSHILDPDIAVLEGGYSI
                     EGALPYMNLGIILAMAGMDYSNVHEPDYDEEKLKQARDITEYIKQLSEDVLEKWKNKE
                     ELGQKKFKGQEIVKRPRRVYYDTDGITEEQVQHFKKCDHCSGVNWIHSSSDSGYKILA
                     ITIPRDACTSCVDQGYEMYENPKSRFTNLYLQDKLGDKYHAK"
     gene            complement(3127..>3939)
                     /locus_tag="VFD08_00990"
     CDS             complement(3127..>3939)
                     /locus_tag="VFD08_00990"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HZK09619.1"
                     /translation="LQMIVEAEEAGLLTPGQTVVELTSGNTGTGLAIVCACKGYRFIA
                     CMSKGNSIERARMMKALGAEVVLVDQMPDSIPGQVSGEDLALVEERAQQLVKEERAFR
                     ADQFNLIACNHAHEFFTGEEMWYQTEGKIDVFVDFLGSGSTFSGCAKTLKKHNPNIRC
                     YVNEPATAAIYAGKEITDKGHDLQGGGYSMDLPLVNKSLIDGYVQVTNDQARYVARKL
                     AQLEGVFAGFSSGANVCAALKLLSGKEKGKNIVVTLNDSGLKYLSTDLFEEV"
BASE COUNT         1277 a          772 c          855 g         1035 t
ORIGIN      
        1 gttataaata ccattaaaaa agtagtggac aaagaaagac tttttgattc tatttggaca
       61 agcttagaag aactattaaa ggattatgat aaatctgaaa ttaaaagaat caatcttagc
      121 actactgtat ctacaaacgc tatcgtggaa gacaaaacat cccctgtggg aatgattatt
      181 caatcaggcc cagggattaa caatgacttt tcatcttgta aagcgcagat cgaggaaatt
      241 actggatata tagaccacag gggcgttatc gttgaaagtt tagacctagt agaggtccag
      301 agggctctag atgcttttaa aaagcaaaag atatcctcct gtgctgtagt cagtaagttt
      361 tctacaagaa atcctaagaa tgaattaagt ataggaaaga tcttagataa agactttgga
      421 aatataagcc taggacatag gatgtcagga aaattaaact tccccagaag agtatatagc
      481 tcttacttaa acgcagcagt atatggtaca ttcaaagatt tttcgacaaa catcaaaaga
      541 tcacttgaaa aagaaggggt aaaatcaccc atatatatcc taaaagccga tgggggaacc
      601 atggatataa agacggcaga aaaaatgcca gtggaaacca ttctttcagg gccagcagca
      661 agttttatgg gaattagcgc aatgcttcct acagaggaag atgccatact cctagatgtt
      721 ggtggtacaa ctacagacat attcttctta gcagatgggg tgccgctatt tgaaccactt
      781 ggggcgcaaa tctctgatta taagaccctg gttcgtgcaa tttatagtgt atccataggt
      841 ttaggtggag atagcgctgt aagtgtagaa gatgctagtc ttaaaatagg cccaagaaga
      901 gagggaagac cctttgcgct aggggggcca aaacccacgc cttctgatgc catgatatat
      961 ttgggtttaa tggatgtatc agaagacctt gccaaagaaa aggccaaaga ggctatggag
     1021 ttgcttgcag ataagttaaa tataagagcc aaagaagtgg cagagcgcat actcactact
     1081 atggcagaga taataaaaaa gaaaaccgac cagctcctaa taaaaataaa tagcaaaccc
     1141 gtctacacca ttggggaatt attgcatgga aaaaaggtga agcctaagaa tttatatcta
     1201 ataggtggtc cagccagggt actatcaccg ctactagaaa agcagtacga tttaccctgt
     1261 cattttcctg caaactttgc tgtggccaat gccatagggg cagccttagc caagcctaca
     1321 atggaaatca ccatgctagc agacacctct caaagaatac taagtgttcc agagctggga
     1381 gtatatgaaa aaatccctaa gaactataca cttgatcggg ccaaggcgaa ggcaagggaa
     1441 ctattgaagt cctacgctct ttctgcaggc cttaaagaag aggattttga atcagaaata
     1501 acagaagaaa gcagttttaa tatggtagat ggtttttata caaagggtaa aaatataaga
     1561 gtgaaagcac aaataaagcc aggacatatt ctcaagctaa ggagtgataa ttaagatgaa
     1621 agccaaaaat aaattaggtt tagttttttt ccctgccttt gattgggcca ttagtccaac
     1681 tcatccagaa agagaagaaa gattactata tacccaagac caaatttttg aagaaggaat
     1741 agaagatatt gaagggattg aaatgttcaa tccccttata gcgcaagaaa aagacattca
     1801 aagagtacac ttttgtgttc ccaatgttac gtccagatta acagagtctc attttatttc
     1861 cgtgggcggg gcaattagag cagcccaggc ggtaatgaac aaggaagtcg acaagtcctt
     1921 tgccttggtc cgtcctccag ggcatcacgc tcatagagta gtctatggag atagaggttt
     1981 ttgtgttgtc aatatagaag caattatgct agaaaaaatg agacaggaat ttggaccctt
     2041 aagagtagcc attgttgata cagattgtca tcatggtgat ggcacagaag atatttactg
     2101 gaacgataag gataccttat ttatatcctt tcatcaggac ggtaggacct tatatcctgg
     2161 aacaggtgcc ataggtgatt tgggaggccc ctcagcctat ggatacaatg taaacattcc
     2221 cctgccgcca cgcacggggg aagagggatt tttatatgta ttaaacaagg tggtaaaacc
     2281 catcttggat gactataagc cagatattat tattaattct gctggacagg ataaccacta
     2341 tagtgatccc attacaaata tgaattttac tgctcaaggc tatgcaaaac tcagtcatat
     2401 cctagaccca gatattgcag tgcttgaagg gggctactcc attgaaggag cccttcccta
     2461 tatgaatctg ggcattatct tagccatggc tggcatggat tactccaatg tccatgagcc
     2521 tgattacgat gaagaaaaac taaaacaagc tagggatata acagaatata ttaagcaact
     2581 atcagaagat gttttggaaa agtggaaaaa caaagaggag ctgggacaaa agaaattcaa
     2641 aggccaagaa attgttaaaa gaccaagaag ggtctattac gatacagatg gaataacaga
     2701 agaacaagta cagcatttta aaaaatgcga tcattgttct ggagtcaatt ggattcattc
     2761 ttcttctgat agtggataca agatccttgc tataaccatt cctagggatg cttgcacttc
     2821 ttgtgttgat caagggtacg agatgtatga aaacccaaaa agcagattca ccaatcttta
     2881 cttgcaggac aagctagggg ataagtatca cgcaaaatag gaagaaattt tagccctaag
     2941 ctgcgcctaa ttggcacaaa atctactggc aaaagaaaaa gaaacaccac tggaaaaaag
     3001 tggtgtttct ttttttgatc cttcttaaga taaggctcct agtaagtgat tactagagct
     3061 ttcttggcta gtagaacact atggtttgcc aggaggaaga tggcagagct tttatcccag
     3121 ctggtcttag acctcctcaa agaggtcggt ggataaatat tttagcccac tatcattgag
     3181 tgtgacgaca atattttttc ctttttcttt tccagatagg agctttagtg cagcacatac
     3241 atttgcacca gaagaaaaac cagcaaagac gccctctagt tgggctagtt ttcttgcgac
     3301 atatcgggcc tggtcattgg taacctgcac gtagccatca attaggctct tattcactag
     3361 gggcaagtcc atggagtagc cgccaccttg caggtcatgc cccttgtcag tgatctcttt
     3421 acctgcatat atggctgcag tggctggctc gtttacgtag cagcggatgt taggattgtg
     3481 cttttttaag gtctttgcac atcctgaaaa ggtgcttccg cttccaagaa aatccacaaa
     3541 aacatcaatc ttcccctctg tttggtacca catttcttcc cctgtgaaaa actcgtgggc
     3601 atgattgcag gcgatcaaat tgaactgatc tgcccgaaaa gccctttctt cttttacaag
     3661 ctgctgggca cgttcttcca ccagggccaa atcttctccg gaaacctggc ctggtattga
     3721 atcaggcatc tggtcaacaa gaacaacttc cgctcccagg gctttcatca tcctagcccg
     3781 ctctatggag tttcccttag acatacaggc aataaagcgg tatcccttgc aggcacatac
     3841 aatggcaagt cctgttcccg tattgccact ggttaattca acaacggttt ggccaggagt
     3901 aagcaggcca gcttcttctg cttccacaat catctgcaa
//
LOCUS       DASRNA010000033         5802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_759511,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000033 DASRNA010000000
VERSION     DASRNA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759511"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFD08_00995"
     CDS             complement(<1..737)
                     /locus_tag="VFD08_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HZK09620.1"
                     /translation="MGLISKVKESKTRIVSEKEKVYLLFLFSLGLMLTALLFNSPAEI
                     YRGMGVILTSKCNLITDYMALANPGAAFFNAGFISMLSLLLIKGSGAHISGASLAAFF
                     TMTGFAFFGKNLFNSIPITLGTYLYSRLVKVNFNTVILSAFYGTGLSPFVSELAFAMG
                     FKPVQGVIVAYAAGIIIGMIFPLLSSRFLSFHQGYNLYNGGFTAGIIGMFSLAILRMF
                     GYDIITADIISHEHSIKILIVLMAIFSL"
     gene            complement(800..1624)
                     /locus_tag="VFD08_01000"
     CDS             complement(800..1624)
                     /locus_tag="VFD08_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZK09621.1"
                     /translation="MVKKGGLGKGLEALLSTNVGESAPRIDYLSLDHIRPNPSQPRKD
                     FDEERLLALSESIKDHGVLQPIVVRRVQGGYEIVAGERRYRASREAGLREIPAIVMNM
                     EPSLQAKLAMVENLQREDLNSVEEAMGYAKLQEEYGLNQKELAELVGRSRVHITNTLR
                     LLGLSPEILAFIKEEKLTAGHGRALLMFPEEKREAMAGLAIEKKMSVRSLEALSKEKK
                     PSKAKAKKDPHIKDMEKRLSHSLGTKVTIVQGKKTGTLEITFYGKEDLELLIEKLI"
     gene            complement(1618..2376)
                     /locus_tag="VFD08_01005"
     CDS             complement(1618..2376)
                     /locus_tag="VFD08_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZK09622.1"
                     /translation="MLKCVAIFNQKGGVGKTTVTLNLAVGLARRKKRVLLIDLDPQSN
                     STSGFGKKAGEKGSIYEVFRGEASMQEVLMKIEANLDLIPSSKDLAAFSMENQTKTPG
                     ILKDKLQSIEEEYDYILIDCPPSLGILSLNALVAAHSVLIPIQCEYYALEGLSQLMET
                     IEMMQRGLNPGLYLEGVLLNMVDKRNNLTKDVAMEVRRFFKDKVYKSEIPRNVRLAEA
                     PSYGMSIYDYDNLSRGSMSFKKWVKEFIKRSETW"
     gene            complement(2491..3162)
                     /gene="rsmG"
                     /locus_tag="VFD08_01010"
     CDS             complement(2491..3162)
                     /gene="rsmG"
                     /locus_tag="VFD08_01010"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZK09623.1"
                     /translation="MKKLHPKLDSIQEEKLSIFRDMVLEKNKVMNLTAITEEDKFYLK
                     HFVDSTKILSFVELKGRVLDLGTGAGFPGIPLAILCPDTEFFLIDSLRKRIRFLEEVV
                     QRLELTNVSLLHARAEDAGRGELRESFDAVVTRALAPLPLLLEYCIPFLKTGANLYAY
                     KGIKLDEELKESKNALHQLKVKVEKVYIYTLGDEEHRILSAKKTKSTPKLYPRRPDEI
                     KKAPL"
     gene            complement(3137..5014)
                     /gene="mnmG"
                     /locus_tag="VFD08_01015"
     CDS             complement(3137..5014)
                     /gene="mnmG"
                     /locus_tag="VFD08_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZK09624.1"
                     /translation="MNYDNYDIIVVGAGHAGVEAALAGARLGNKVLLLTMNLDSISLL
                     ACNPSIGGTGKGHLVREIDAMGGEMGLVTDKVTLQSKMLNTAKGPAVHSLRVQADKNK
                     YHQEMKKVLEEEENLDLRQAEVTQLLIEDKKVLGVKAVGLEFKARAVILATGTYLRSL
                     CFIGDAVLDSGPSGLYNSKKLSEDLENHGFALRRFKTGTPARIHGKFVNYNKLIPQWG
                     DEKIVPFSFMNDSLVFDEFPCFLGYSNTTTHQIIQENIHLSAMYSGNISGTGARYCPS
                     IEDKITRFAGKERHQLFLEPEGADTCELYVQGMSSSLPERVQEEFLSSIEGFEKVKIM
                     RPGYAIEYDCIDPTVLELSLESREIENLFFAGQINGSSGYEEAAAQGLLAGINADLKL
                     KGKDPLILHRDQAYMGVLVDDLVTKGTDEPFRMMTSRAEYRLVLRQDNADFRLTSIGY
                     DLGLVSKQRYERMQKRKEAFEREMQRLEQTVPLADVNAYFEKRGLGLLHSSPKIITLL
                     RRSDMTYKDLANLNLTDDCPEDVQTMVEIEVKYKGYIDKQMRQVEQFRNLEDKLLPKD
                     IDYLIIDNLRIEARQKLQAAKPSSIGQASRISGVSPADITVLLIHLEKRRRSEKTPSQ
                     A"
     gene            complement(5015..>5802)
                     /locus_tag="VFD08_01020"
     CDS             complement(5015..>5802)
                     /locus_tag="VFD08_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZK09625.1"
                     /translation="GMEKVYKELALLLENSKKGKLLEEGLRTVISGRPNVGKSTLLNA
                     LSGHSRAIVTEIAGTTRDTIEERIQIAGLPLILVDTAGLRQTLDPVEHLGVERSQAAM
                     EKADFILYVLSADERLQEEDISRLKLLENREYLVIVNKTDKNMSIELDKLYSIVNKDN
                     IIFTSLLKEQGIIDIEEAILERTIGKELSLSEPLIQNQRQIALVKESIVWCEDALADL
                     ARKEAYDYVEVNIKGCFESLGLLLGENNPDIIGEVFSRFCVGK"
BASE COUNT         1635 a         1294 c         1047 g         1826 t
ORIGIN      
        1 aggctaaaga tagccataag tacaataaga attttaatgc tatgctcgtg agagataata
       61 tctgcagtta taatatcata accaaacatt ctaaggattg ctaaggaaaa catgccaata
      121 attcctgctg taaagcctcc attatacaaa ttataccctt gatgaaagga taaaaaacgc
      181 gaagataaaa gcggaaaaat cattcctata ataatgccgg cagcataggc aacgataact
      241 ccctgtactg gcttaaaacc catggcaaaa gcaagttcgc ttacaaaagg agacaggcct
      301 gttccataaa aggctgaaag aataacagta ttaaaattaa ccttaacaag tcgcgaatac
      361 agataggtcc ccaaggtaat gggaatagag ttaaagaggt tttttccaaa aaaagcaaaa
      421 cctgtcatag taaaaaaagc tgctaaactt gctcccgaga tatgtgctcc actaccttta
      481 attaaaagta gggacaacat actaataaat cccgcattaa aaaaagccgc tccggggtta
      541 gccaaagcca tataatcagt aataaggttg cactttgagg ttaggattac tcccattccc
      601 ctataaatct ccgccggact attaaaaagt aaggcggtta acatcagacc tagcgaaaaa
      661 agaaataaga ggtatacctt ttctttttcg cttacaattc gggtttttga ttcttttact
      721 ttcgaaatca gccccatacc tactcccttt cagctaatgt ttcacgtgaa acattgtcca
      781 ggtgtttcac gtgaaacatc tagatgagtt tttctataag taactccaga tcttccttac
      841 catagaaggt aatctccaga gtgcctgttt tttttccttg aacaatagtc accttggttc
      901 ccaagctgtg actcaatctt ttttccatat ctttaatatg ggggtctttc ttagcttttg
      961 ctttgcttgg ttttttttcc ttgcttaggg cttcgagact tcgaaccgac atctttttct
     1021 caatagcaag accagccata gcttcccttt tttcttccgg gaacattaaa agagccctac
     1081 cgtgacccgc tgtaagtttc tcttctttaa taaaggctag aatttctggg gaaagaccta
     1141 ataatctaag tgtatttgtt atatgaaccc tactcctgcc aacaagttca gctaattctt
     1201 tttgattaag tccatattct tcttgcagtt ttgcatagcc catggcttct tccacactat
     1261 taaggtcttc tctttgtagg ttctccacca tggccaactt ggcctgtaaa gaaggctcca
     1321 tattcataac aatagcagga atttctcgga gaccagcttc tctacttgcc cgatagcgcc
     1381 gctctccagc tacaatttca taccctccct ggaccctgcg aaccacaata ggctggagga
     1441 ccccatgatc cttaattgat tctgataagg ctagaaggcg ttcctcatca aagtcttttc
     1501 ttggttgaga ggggttggga cggatatgat ccaaagaaag atagtcaata cgaggagcac
     1561 tttctccaac attcgtagat agtaaggctt caagcccctt acctaggccc ccttttttta
     1621 ccatgtctcg ctccttttta taaattcctt gacccatttt ttaaaactca ttgagcctct
     1681 ggataagttg tcgtaatcgt aaatactcat tccataactt ggcgcttcag ctaatctgac
     1741 atttcgagga atttcgcttt tatatacctt gtccttaaaa aaccttcgca cctccatagc
     1801 cacgtcctta gtcagattat tgcgcttatc aaccatattt aaaagaactc cttcaagata
     1861 aagtcctgga ttgagtcctc tttgcatcat ttctatggtc tccattagct gggagagccc
     1921 ttcgagggca tagtactcac actggatggg gattaataca ctgtgggctg ctactagggc
     1981 attcaaagac aagattccta gggagggagg gcagtcgatc aaaatataat catactcctc
     2041 ttcaatggat tgaagtttat cctttaaaat tcctggtgtc tttgtctggt tttccataga
     2101 aaaggcagcc aagtccttgg aggaggggat aaggtctaag tttgcttcta ttttcataag
     2161 gacctcttgc atagatgcct ctccccggaa aacctcataa atgctgccct tttccccagc
     2221 ttttttacca aaaccactag tactattact ctggggatct aaatcaatta aaaggactcg
     2281 ttttttacga cgagctaggc ctacagctag atttaaggtg accgtagttt tacccacgcc
     2341 ccccttctga ttaaagattg caacgcattt tagcataatt cacctcggga ccattgtacc
     2401 aaagaaaacg agtcctatgc aaaccaaatt agaataaaaa acagagtgaa ataaatcact
     2461 ctatttaatc cgcttttggt ccagaccctc ctatagaggg gcttttttta tctcatctgg
     2521 tcttctgggg taaagttttg gggtggattt tgtcttttta gccgatagta tccggtgctc
     2581 ctcatctcca agagtatata tatagacctt ctcaaccttg acttttaact gatgcagagc
     2641 atttttcgac tcttttagct cttcatccag ctttattccc ttataggcat ataaatttgc
     2701 tcctgttttt aaaaagggaa tacaatactc cagcagtaag gggaggggag ccaaggcacg
     2761 agttaccact gcatcaaaac tttctcttag ttctccccgc cctgcatctt cggccctggc
     2821 gtggaggagg ctaacattgg taagttcaag cctctgtacg acttcttcaa gaaagcgaat
     2881 tcgttttctt aaagagtcta tcagaaaaaa ctccgtatct ggacaaagaa tagcaagggg
     2941 tatccctgga aagccagctc ctgttcctaa atcaaggact ctacctttaa gctcgacaaa
     3001 actaagaatt tttgtgctat caacaaagtg cttcagataa aacttatcct cttcagtaat
     3061 agcggtcaaa ttcataacct tgtttttttc taaaaccata tccctaaaga tagataattt
     3121 ttcttcttgt atagaatcaa gcttgggatg gagttttttc actgcgtctc cttttttcta
     3181 aatgtattag taatacagta atatcagcag ggctcacccc gctaatgcga gaggcttggc
     3241 ctatgctgct gggctttgct gcctgcagtt tttgccttgc ttctatacgc aagttatcaa
     3301 tgattaaata atcaatatcc tttggcagga gcttatcttc aagatttcta aattgttcta
     3361 cctggcgcat ttgcttatca atataaccct tgtattttac ttcaatttca accattgttt
     3421 gtacatcttc tggacagtca tctgtaagat taagatttgc aagatcttta taggtcatat
     3481 ccgagcgtcg taatagggta atgattttcg gagacgagtg taaaaggccc agacctcttt
     3541 tttcaaaata ggcattgaca tctgctaggg gaacagtttg ttctaagcgc tgcatttctc
     3601 gctcaaaggc ttcttttctt ttttgcatgc gctcgtagcg ctgcttgctt acaagaccaa
     3661 gatcataccc aatactagtt agacgaaaat ctgcattatc ttgacgcagt accaaacgat
     3721 attcagccct tgaggtcatc attctaaagg gttcatcggt tccctttgtt acaagatcat
     3781 ccacaagtac tcccatatag gcctggtctc ggtgcaagat taaaggatct ttgcccttta
     3841 attttagatc tgcattgatc ccagccaaaa gaccctgagc agcggcctct tcatagccgc
     3901 tggatccatt aatttgtcca gcaaaaaata aattttcaat ttctctactc tctaaggata
     3961 gttctagcac tgtagggtca atgcaatcat attcaatagc ataacccggt ctcatgattt
     4021 taaccttctc aaagccctca atggaagaga gaaattcttc ttgcactctt tctggaagag
     4081 aagaactcat tccctgcaca tagagctcac aagtatcagc tccttctggt tctaagaaaa
     4141 gctgatggcg ctcttttcca gcaaatctgg ttattttatc ttcaatagaa gggcagtagc
     4201 gggctcctgt gcccgaaata ttcccactat acatagctga aagatggata ttttcttgaa
     4261 taatctggtg ggtggtggta tttgagtaac caagaaaaca aggaaattca tcaaaaacca
     4321 aactatcatt cataaagcta aagggaacga ttttttcatc tccccactgg gggatgagtt
     4381 tattgtaatt tacaaatttc ccatggatcc gagctggggt tcctgtttta aatcttcgta
     4441 gggcaaaacc gtgattttct aaatcttcgc ttagtttttt actattatat aggccactgg
     4501 ggccagaatc taagactgca tcaccaataa aacacaaact tcttagatag gtacccgtgg
     4561 caagaataac tgcccttgct ttaaactcaa ggcctacagc cttaacacca agtacttttt
     4621 tatcttcaat aagtagttgg gtaacttctg cttgacgcaa gtctagattt tcttcttctt
     4681 ctaatacttt tttcatttct tggtgatatt tattcttatc tgcctgcacc ctaagggagt
     4741 gaaccgctgg acccttggct gtattgagca ttttagactg cagggtaact ttgtcggtga
     4801 caagtcccat ttctccgccc atggcatcta tttcgcgcac caagtgaccc ttgcccgtgc
     4861 ctccaatgga gggattgcag gcaagaaggg aaatagaatc taaattcatg gtaagaagca
     4921 gcaccttatt gcccagtctt gcaccagcaa gggcagcttc tacaccagca tggccagcac
     4981 ctactactat tatgtcataa ttgtcataat tcatttattt acctacacaa aatctactaa
     5041 atacctctcc tataatatca ggattgtttt ctccaagaag aaggcccaag ctttcaaaac
     5101 accctttaat attgacttca acataatcat aagcttcttt tctggcaaga tccgctaggg
     5161 catcttcaca ccagactatg gattccttga ctaaggcaat ctgcctttga ttttgaatta
     5221 aaggttcgct taaactaagc tctttgccaa tggttctttc taaaatcgct tcttcaatgt
     5281 cgattatacc ctgttctttt aagagggaag taaaaataat attgtcttta ttgacaatag
     5341 aatatagttt gtcgagttca attgacatgt ttttatctgt tttattaact ataaccagat
     5401 actctctgtt ttcaagtaat ttaagtctag atatatcttc ttcctggagc ctctcatctg
     5461 cactaaggac atagagaata aaatccgcct tctccatggc agcctggctt ctctcaacac
     5521 caagatgctc cacaggatct agggtttgtc ttagccctgc tgtatctact agaataagag
     5581 gaagacctgc aatttgaatt ctttcttcta ttgtatctcg agttgttcct gctatttctg
     5641 ttacaatggc gcgactatgg ccacttaggg cattaagaag ggtagatttt cccacattgg
     5701 gccttcctga aatcactgtg cgaagaccct cttctagaag tttacccttt tttgagtttt
     5761 ctaagagcag ggctagttct ttgtatactt tttccatacc at
//
LOCUS       DASRNA010000034         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_383288,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000034 DASRNA010000000
VERSION     DASRNA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383288"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /locus_tag="VFD08_01025"
     CDS             <1..1885
                     /locus_tag="VFD08_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HZK09626.1"
                     /translation="LHASTQLSIHSRPMADYFKQKGARRLILARELSLEEIEKIKGVA
                     DLEVFVQGALCISYSGQCLMSSLIGGRSGNRGRCAQPCRLEYGLYEEKDLIKKGNLLS
                     PRELSLLVDELSALKKAGVVSLKIEGRMRSPGYVYEAVRIYRQALNKEAYDDQSLKLS
                     FSRQGFTKAYLYKKGGEDLISEFSGKSGIDLGYIEKGEITLLQDIRRLDGVGTPQGGF
                     TIEGILYQGKKVEEAFVGQRVGLLPKKYKEGDLIRKTFDSQEEKKIKKIVKEPYKRKL
                     ALKVPFYFKIGEPMVLGHLKGDLVQEARTAPLSKQRLIESLSKSDGPLFIEPDFIDFE
                     EGFVPIREINLLRRRFLEEALEKRKVKRKLEKKEFPSIDKKEALAKKFVILREKKNLE
                     LDLMGMVPIYDPFFKDPGSLSFKDLAPLENYLLRVPGLVKEKPEELCDKLLSLEGLRG
                     ILTSNQGIIELMKNKIPLYGDYKLNIINSYGLALYKELEGSILSEEMSKNELKSFQGK
                     ERAMIYSYGPQEMMVMEHCLLRDAEPCSEPCRVKNYSLEDRKDYKMKMAHDIYCRNHI
                     YNAPVKNLLGESKELRKMGYSSFVMEIFQEEDPQELIEAFYDEKPLERDLATWGHYHR
                     GIL"
     gene            1957..2592
                     /locus_tag="VFD08_01030"
     CDS             1957..2592
                     /locus_tag="VFD08_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK09627.1"
                     /translation="MFVRAIMTPVTELTTVSSDQKLKDALDIILEEGFLSLPVVDGEK
                     FVGYISKMYIYEKFIESGGKDFDAYLQEKVASYAFMDMEPLMDSTYVEDAAEAFFTNK
                     VMFIPVVDSREVFRGVVTQRSLFKVILKVFGLKDAKITIFSQDFVGSLAEILDIIQRH
                     GSIVTNIAKYDMDILGTQGITIRVEDKDPEVLVEKLKAKGVNVREFQPARK"
     gene            2561..3247
                     /locus_tag="VFD08_01035"
     CDS             2561..3247
                     /locus_tag="VFD08_01035"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZK09628.1"
                     /translation="MFENFNQQGNSMDYPREQKSFSLWILLLSFASGYANVAAMTRFN
                     QPVSHMTGTVSSLALAIFEREAFLVERLLSLLMCFLAGAIFAGILFHKRKIEPKKRYG
                     ALLFIGGVIIFVLKNSEVLFYVLGFFMGLQNAMLLDYRGHLIRSTHITGYLSDIGFEM
                     GSFLVGEPRHAWKIVFYTLSIFLFVLGGLFSVYFYSQGNQVELLSGIYILLGSSYFYL
                     RREELLKKKQ"
     gene            3267..4412
                     /gene="rlmD"
                     /locus_tag="VFD08_01040"
     CDS             3267..4412
                     /gene="rlmD"
                     /locus_tag="VFD08_01040"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZK09629.1"
                     /translation="MRNKECVHFKDCGACHFRLAFPKGQTKKKQEYIERLFPDKKVAP
                     ILAMENPYYYRNKVLRTFKSVGKHRFISGIYEKGSHWVVPIDQCLIEDEGAKAIQRTI
                     YSLVKRFKWTIYDEDLGQGLVRHALVRVAKATNQASVTMVIKGDFLPKQDEFVRALTK
                     RHPQIKSIYINPHKRKTPVVVEGPLHQIYGKGPIKDHLLGLDILLSGDSFYQVNHLQA
                     EKLYTKVMEVLDVQKEEVILDAYCGIGTMALLAAKKGARLIGVDNNASAMSDARQMKK
                     INKLNKVDFIEEDATKFMEKLASTGDKVDKIILDPPRSGTTEAFIEASLSLVPEKIVY
                     VSCDPARFKEELPLFEKDYQVEGIYPVDLFPFTRHVETVVLMSRVEK"
     gene            5147..5617
                     /locus_tag="VFD08_01045"
     CDS             5147..5617
                     /locus_tag="VFD08_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK09630.1"
                     /translation="MFRKMRRFKQEISEQECIDTLKEQPRGVLAVYGEDGYPYAFPMD
                     YIYMNEKLYFHCAKEGHKLDAIAADNRVSFCVMDEGFRKDGDWALNIRSIVIFGRVKR
                     IDDADETLKVVRQLGLKYYPTAESVEEEIRKAIARVQILELSIDHMTGKLVNES"
     gene            5683..>6093
                     /locus_tag="VFD08_01050"
     CDS             5683..>6093
                     /locus_tag="VFD08_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZK09631.1"
                     /translation="MPENKKVHYIPAKPPKRDKRVGIYCRVSTNSVDQLKSLTAQVSA
                     LTRLTAATPQWLLVDVYMDIASSKTGSSRREFARMLDDCQSHNLEIILTKSISRFGRD
                     TVDTLEALNQLKTLGVRVIFEQEDLDTSNTDSDLM"
BASE COUNT         1991 a          988 c         1364 g         1750 t
ORIGIN      
        1 gctccacgca tcaacccagc ttagtattca tagtaggcct atggcagact actttaaaca
       61 aaaaggagcc agacgcctaa tccttgccag ggaattgtcg ctagaggaaa ttgaaaagat
      121 taagggggtg gctgatctag aggtctttgt tcaaggagct ctatgtatca gctactcagg
      181 ccagtgccta atgagcagcc ttatcggtgg ccgcagtggc aatagaggtc gctgcgccca
      241 gccctgtagg ttggagtatg gtctttatga agaaaaggat cttatcaaaa aaggaaacct
      301 tctaagcccc agggaattgt cccttcttgt ggatgagctt tctgctctta aaaaggcagg
      361 ggtcgtgagt ctaaaaatag aagggcgcat gagaagccca ggctatgtct atgaagcggt
      421 tcgtatctac cgccaggctc ttaataaaga ggcctatgat gatcaaagcc taaaacttag
      481 ctttagccgc cagggcttta ccaaagccta tctttataaa aaaggcggag aagaccttat
      541 ttctgaattt tcaggaaaaa gcggcatcga cctgggttat atagaaaaag gggaaattac
      601 tcttttgcaa gatattaggc gccttgatgg agtaggcacc ccccagggtg gttttacgat
      661 agagggcatt ttgtatcaag gaaaaaaagt agaagaggcc tttgtgggcc aaagggtagg
      721 tcttcttcca aaaaaataca aagaaggaga tctaataaga aagacttttg atagccaaga
      781 agaaaaaaag ataaagaaaa tagtaaaaga accctataag cgcaagctag cacttaaagt
      841 gcccttttat tttaagatag gagagcccat ggttcttggt catcttaagg gtgatcttgt
      901 ccaagaagca agaacagccc ccctaagcaa acagcgccta atagaaagtc tctccaagag
      961 cgatggccct ctttttattg agcccgactt tattgatttt gaagaaggct ttgttcccat
     1021 aagggagata aatttactca ggcggcggtt tttagaagag gccctagaaa agaggaaagt
     1081 caaaaggaag ctagaaaaaa aagagtttcc ctctatagac aagaaagaag cccttgcgaa
     1141 aaagtttgta atattgcgag aaaagaagaa tttagaactt gatttaatgg gcatggtgcc
     1201 catttacgac cccttcttta aagatccagg aagtcttagt tttaaagacc ttgcaccttt
     1261 agaaaattat ttgcttaggg tgccaggact tgtaaaagaa aagccagaag aactctgtga
     1321 taagctcctt tctttagagg ggctaagggg tattttaacc tccaaccaag gtattattga
     1381 gcttatgaaa aacaaaattc ctctttacgg agattataaa ttaaatatca tcaacagcta
     1441 cggccttgct ctttataagg agttagaagg cagtatctta agtgaagaaa tgagcaaaaa
     1501 tgaattaaaa agctttcagg gaaaagaaag agcgatgatc tatagctatg ggccgcaaga
     1561 aatgatggta atggagcatt gcctgctaag ggatgcagag ccttgtagtg agccttgccg
     1621 ggtaaaaaac tatagtttag aagatagaaa agactataag atgaagatgg cccatgatat
     1681 ttattgtaga aatcatatat ataatgcccc agtgaaaaat cttttgggag aaagcaaaga
     1741 actgaggaag atgggttata gtagttttgt catggagatc ttccaggagg aagatcccca
     1801 ggaactaatt gaggcttttt atgatgaaaa acccttggaa agagatcttg ctacctgggg
     1861 acactatcac cgcggcatcc tatagtactt gtctcttagc ttagaaaagc ttatactaca
     1921 aataataaac aacttaaaag gatataagga ggagctatgt ttgtacgtgc cataatgacg
     1981 ccggttactg aattgactac cgtttctagt gatcaaaaac taaaagatgc cttagatatt
     2041 attctcgagg aaggttttct ttccctgcct gtggtagatg gagaaaaatt tgtgggctat
     2101 atttcaaaaa tgtatatcta tgaaaagttt attgaaagtg gaggaaagga ttttgacgcc
     2161 tatcttcaag aaaaagtggc cagctatgcc tttatggata tggaacccct tatggactcc
     2221 acctatgtag aggacgctgc agaggctttt tttactaata aggttatgtt tattcctgtg
     2281 gtagatagta gagaggtttt ccgaggggtt gttacccagc gctcgctttt taaggttatt
     2341 ttaaaggtct ttggcctaaa agatgccaaa attaccatct ttagtcaaga ctttgttgga
     2401 agtttagcag aaatcttaga catcattcag cgccatggta gcattgttac caatatagct
     2461 aaatatgata tggacattct aggaacacaa gggataacca ttagggttga agataaggat
     2521 cctgaagttc ttgtggaaaa actcaaggca aaaggagtta atgttcgaga atttcaacca
     2581 gcaaggaaat agtatggatt atcctcgaga gcaaaagtcc tttagtttgt ggatactgct
     2641 tttaagtttt gctagtggct acgccaatgt tgcagcaatg acacgtttta accagccagt
     2701 aagtcatatg accggaacgg tctcctcact tgccttggct atttttgaga gggaagcctt
     2761 ccttgtggag aggcttcttt ctcttcttat gtgttttcta gcaggggcaa tttttgcagg
     2821 tattcttttt cacaaaagaa aaatagagcc taaaaaacga tatggagccc tactttttat
     2881 tgggggtgtg attatctttg tcttaaaaaa ctcagaagtc ttattttatg ttcttggttt
     2941 ttttatgggt ctacaaaacg ctatgctttt ggactaccgg ggacatttaa ttagaagcac
     3001 tcatataaca ggttatttaa gtgatatagg ttttgagatg gggagttttc ttgtaggaga
     3061 gcctaggcat gcttggaaga ttgtatttta taccttaagt atcttcctat ttgttttagg
     3121 aggattattt tctgtttatt tttattccca gggtaatcaa gtggaacttt taagcgggat
     3181 ttatattctt cttggaagca gctattttta tcttagacga gaggagcttt taaagaaaaa
     3241 gcagtaagga cataggaggt ccactaatga gaaacaaaga atgtgtgcat tttaaggatt
     3301 gtggggcctg tcatttccgc cttgcttttc caaagggaca gactaagaaa aaacaagaat
     3361 atatagaaag actttttcca gacaaaaaag ttgcccctat tttagctatg gaaaacccat
     3421 attattaccg caataaggta cttcgaacct ttaaatccgt gggcaaacac cgttttatta
     3481 gcggaattta tgaaaagggg agccactggg ttgttcccat tgaccagtgt ctgattgaag
     3541 acgaaggggc caaagcgatt caaagaacaa tttatagtct agtaaagcgc tttaaatgga
     3601 ctatatatga tgaagaccta gggcaaggcc ttgttcgaca cgcccttgtt agggtagcta
     3661 aagccacaaa tcaagccagt gtaacaatgg ttatcaaagg agattttctt ccaaaacagg
     3721 atgagtttgt aagggcttta acaaaaagac acccccagat aaaaagcatt tatattaacc
     3781 ctcataaaag aaaaacaccg gtggttgtcg aaggcccttt gcaccagatc tatggaaaag
     3841 gaccaataaa agaccatctt ttagggctgg atatacttct ctcaggagat agtttttatc
     3901 aggtcaatca tttgcaagca gaaaaactct acacaaaagt aatggaagtg ctggatgtgc
     3961 aaaaagaaga agtaatccta gatgcctatt gtgggatagg aaccatggca cttttagcgg
     4021 ccaaaaaagg agcacgacta ataggagtgg ataataatgc ttcagccatg tctgatgcca
     4081 ggcagatgaa gaaaataaac aaactaaaca aggtcgactt tatagaagaa gatgcaacta
     4141 agtttatgga aaagcttgca agtaccggag ataaggtgga taaaatcatt ttagatcctc
     4201 caagatctgg gaccacagaa gcttttatag aagcatccct aagcctggtg ccagaaaaaa
     4261 ttgtttatgt atcttgtgat cctgccaggt ttaaagaaga gctccccctt tttgaaaagg
     4321 actatcaggt cgaaggaatt tatccagtgg atctttttcc tttcacaaga catgtggaga
     4381 cagttgtatt gatgtcaagg gttgaaaagt aaaaggctta aaattgtagt aaataagggg
     4441 ttctggttgt tttgctgatt tgcagaatgg cagaaccctt tgtaaaatcg gtactttgtg
     4501 ggaatatatc aaccgtaaaa aatgcgtata gttgagttga tagaattact tttgggtagg
     4561 ttgagttgac ggaaatgttg taaaaatagg ctaatcatcg tataataaaa tcattggaat
     4621 gatgagtagc ctcgagggta ttacagaccg attgaccacc caatgttata gatatttgca
     4681 ttctcttttg atggagtaaa agatatcaac atccaatctt cgtttttaca attggcatta
     4741 tcttcgtaaa attgttgaag agtaaggctg attagtagga tagcgaaggt atatatttgc
     4801 atagcatttg ttaatgttct tatgatttca tttgttatga cagcatcata aagcatttct
     4861 tgttgtcctc ttttttcttc atgagaatca agagtatcaa tgcatatatc tgcataaggg
     4921 atgcaatggt cttgccgctg ggaataagtg gctaattgtt ttttagttct ttctttattg
     4981 ctgatgaaca atttttatag cagatggaaa cgtgtaatat cggtctttta ttcatgacga
     5041 tacctccttc ggtagtgtca catattaact ctaaagacct atattatcaa gatgtatatt
     5101 gaacaaataa ataggaattt gaactaagaa atgaagggag attattatgt ttcgtaaaat
     5161 gagaagattt aaacaggaaa tatctgaaca agaatgcatt gacacattaa aagagcagcc
     5221 aagaggagtc ctagcagtat atggggaaga tggttatcca tatgcttttc caatggatta
     5281 tatctatatg aatgaaaaac tgtactttca ttgtgcgaaa gaaggacata aacttgatgc
     5341 tatagctgcg gataacaggg tatcattttg tgttatggac gaaggttttc gtaaggacgg
     5401 ggactgggca ttaaatatca gaagtatcgt tatctttggc agagtaaaaa ggattgatga
     5461 tgccgatgaa acattaaaag ttgttcgtca acttgggctt aaatattatc caacagctga
     5521 atctgttgaa gaagaaattc gtaaagcaat tgcaagagta caaatcttgg aattatccat
     5581 tgatcatatg actgggaaac tggtaaatga gtcataggtt ttagaatgct tgttaaattc
     5641 caatttgaat ggaattttaa gaattatttg gaggtatcaa gtatgccaga aaacaaaaaa
     5701 gttcattaca taccagcgaa acctccaaag agagataagc gagtgggtat atactgccgt
     5761 gtaagtacaa atagtgtaga tcaactaaaa agcctaacag cacaagtatc ggcactaaca
     5821 agattaacag cggcaacacc acaatggtta ctggtggatg tatatatgga cattgcctcc
     5881 agtaaaacag gttcttctcg tagagagttt gcaaggatgc tggatgattg ccaatctcat
     5941 aatctggaaa tcatactcac taagagtatt agtcgatttg gtagggacac agtggataca
     6001 cttgaagcgt taaaccagct aaaaacttta ggggtgcgag ttatatttga gcaagaagat
     6061 ttggatactt caaatacaga tagcgacctt atg
//
LOCUS       DASRNA010000035         8568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_12541,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000035 DASRNA010000000
VERSION     DASRNA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8568
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12541"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..374
                     /locus_tag="VFD08_01055"
     CDS             39..374
                     /locus_tag="VFD08_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZK09632.1"
                     /translation="MKKIVMYSSPSCPHCHTARDFLQKEGIPFIDKNVQNPKHQKEFQ
                     ALGIEGVPAFLIGEEVMVGFNSSQLLSKLDFLLPQCPSCGRKMMVPKGKGKIKVTCPK
                     CKTKFEMQS"
     gene            383..1819
                     /locus_tag="VFD08_01060"
     CDS             383..1819
                     /locus_tag="VFD08_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK09633.1"
                     /translation="MNYTSERERRMATESIPRLFFSFSLPAIAGMMVQALYNIVDRFF
                     IGQIPGTGSIAIGGIGVSLPITFILMGFTMLFGIGGGANISIQLGKKDQPMARKILAN
                     ALIMLFLSALFLNGFMLLNLEKVLLIFGASAQNLPYASSYLRIILIGNYWNTFAFAFN
                     HLIRAEGNPKFAMQSMLIGAITNIILDPIFIFVLDMGIAGAAYATIIAQFLSFLRGFS
                     YYLGGKNIVNLKKEDFRLESKVIYMIVAIGISPFFIQIAGSVVGALLNNSLRIYGGAL
                     AQGAYAIINSIAMLFFMPIFGMNQGLQPIVGFNYGAGNFKRVKEAVAVGIISASTVMG
                     LGWIVVQFFPHVLVGVMTPDPELFDITVKGLRIFLSMMFVAGFQIISSNFFQSIGKAK
                     ISFVLSLSRQVFLLAPLVYFLPRFFGLKGVWYAQPIADALSSLITLFFILREFRSLEW
                     AHCQSNPLAEGCEENLEVLRAIKVQEKT"
     gene            1939..3324
                     /locus_tag="VFD08_01065"
     CDS             1939..3324
                     /locus_tag="VFD08_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK09634.1"
                     /translation="MFGKKPSLDASTHAIHRKEVIKIAWPVLVELVLASLFSMVDMMM
                     LGNIPSRIIANQSVAAVGIVNQPLFIGISLVQSVNVGCTALVSRYVGSREEHKIEDTL
                     KHVLLISFIGLAIPYFILMQIFRDPIMTFLGAEPAVLALGGSYFRILISGFLFQALNF
                     SLTAALRGAGETKVPMKVNIFANLLNVFGNAILIFGLLGFPALGITGAAISTALSNLL
                     AFILMYSYVIAGKSKVKLSLGRFTLQTSVFLNLARIGVPASMEQFVLRVGIMLFTKIV
                     AGLGTAIFAAHQIAISILGLSFNPGMAFGIAASALVGRALGEEDAELAASYANDARKL
                     GSRVAGVVGILLFVFAPQLVSLYNKDPLVIKNASIALRIIAAIQPIQSSQLILAGALR
                     GAGDTFSTFLATLISLLVIRVSLAYLFVHVLFYGIAGAWAAVVVDQFIRWMIVRTRFK
                     RGRWKTLTFHS"
     gene            3496..4059
                     /locus_tag="VFD08_01070"
     CDS             3496..4059
                     /locus_tag="VFD08_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK09635.1"
                     /translation="MNAKKSIKVAFWDLLQELPLDQITVIMLCERANVNRQSFYYYYQ
                     NIVDLLKDVLFAEIYDEVSQGRAYDTWKYGFYTTTRFINENHFIFQNIQHSNYWEEIN
                     NFFTENSNSLVKGVIDECIEATGIRVSKEDKDFMIHYYRVLFNSIMTEWVKDGMKLKP
                     IELLSKVEKLVDGTICPALQRFSEEEN"
     gene            4434..7163
                     /locus_tag="VFD08_01075"
     CDS             4434..7163
                     /locus_tag="VFD08_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002683765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK09636.1"
                     /translation="MSKKIPYTVDYSPKRRSPILLDFANLINRTKTGDKGALTYEDPE
                     YYVMENIVTDQMAKVGLGLRLRVYQTAEQIAKTVQMPVEVVRDRLEDLAMAGASHRAT
                     IDGEVKYWLEIWVPGHMEMIMNNRENAKKYPEIAIGFEAYGKKKGIIAPGSFPMGMAP
                     MRVIPIESAIDGNSRTASYEEISTYLNKHTIFSVSDCSCRTVREEMGEGCGHLKEDMC
                     IQMGTAADYYIHTGRARQITREEAFEIIHKAEENGLLHQIPNLDGSGETHAICNCCGC
                     GCLALRNANMFRNPDFSRSNYVAKVDSEKCVACGECVEHCPVNALKLGQKIPATVALA
                     EVDKHEDRPGNTKWGIDKWNIDYRENREVVMDSGTSPCKSFCPAHIGVQGYIQLASEG
                     KYGEALELIKQENPFPAVCGHICPRYCEDACTRNSIDDSVAIDDIKRFIAEQDMNSEH
                     RYVPKKKHDYSDKKVAVVGAGPAGLSCAYYLAIDNYDVTVFEKEEALGGMLTWGIPSF
                     RLEKEVINSEIDVLKELGVKFQTGVEIGKDLSLDDLRQQDYKAFYLAIGAQGGRKLGI
                     EGEDAQGVYSGVDFLRKVNLTEERLLSGNTVVIGGGNVAIDVARTAVRSGSKQVQMVC
                     LESPEEMPALPEEILEAQEEGIEVLNGWGPKRILTKDGKVTGIEFKRCTRVFDENQRF
                     SPLYNEEETYFLEADNILVSVGQSIVWGDLLKGSKAEVNPNQTLPADGTTFQTAQEDI
                     FVGGDAHTGPKFAIDAIAQGKEGAISIHRFVQHGQSLVLGRLRRNYLALDTETVDYQG
                     FDLKPRQEAEKVDGSVSRTSFRDLSKSFTPEQIQQEADRCLSCGKTYVDEHMCVGCGQ
                     CTTNCRFDAISLEKVYDAEGMELSKVKPYVVRNVLKTKSKIFVHSAKKKIMGK"
     gene            7171..7509
                     /locus_tag="VFD08_01080"
     CDS             7171..7509
                     /locus_tag="VFD08_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HZK09637.1"
                     /translation="MHELGIMIEVVKQVEEVARENQAEKVEKIVLQIGEISTVIPHYV
                     EEVFPAAVNKTMLEDTQLEIEIIPANGRCLECGKVFSLVSQKGKCTCGSEDFEELTGR
                     EFILKEIQIS"
     gene            7519..8187
                     /gene="hypB"
                     /locus_tag="VFD08_01085"
     CDS             7519..8187
                     /gene="hypB"
                     /locus_tag="VFD08_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HZK09638.1"
                     /translation="MSAIRVIEIKKGIFEQNDKKAEKIRKELKDKGLFLLNLMSSPGS
                     GKTTTLVSTIEQLKDEMEIGVIEADIDSDVDARTIDKTGVRVIQLHTGGMCHLDAEMT
                     RQGIEALGAEGLDFMVLENIGNLVCPAGFDTGATKNAMIISIPEGHDKPLKYPEMFKV
                     VDVVLVNKIDAMEYFDFDLDLFRDFVHKINPEIEIIPMSAKTGEGIEPWVDWIRKQVA
                     KQKV"
BASE COUNT         2604 a         1496 c         1932 g         2536 t
ORIGIN      
        1 ttagggctac ttatagctag catgttcagg aggtatctat gaaaaaaata gtaatgtact
       61 caagtccgag ctgtccccat tgtcatactg ctagagactt tttacaaaaa gaagggattc
      121 cctttattga taagaatgtt caaaatccaa agcatcaaaa ggaatttcaa gcccttggta
      181 tagagggtgt tcctgctttt cttattggcg aagaagtgat ggtaggcttt aattcctccc
      241 agctcctttc caagctagac tttttgcttc cccagtgccc ttcttgtggt agaaaaatga
      301 tggtgccaaa gggcaagggt aaaattaaag tgacttgccc aaagtgtaag acaaaatttg
      361 agatgcaaag ttaggagttt atgtgaatta tactagtgag agagaaaggc gaatggccac
      421 agagtccatt cctcgcctgt tttttagttt tagcctgcca gctattgctg gcatgatggt
      481 acaggcctta tacaatatag tggaccgctt ttttattggt caaattccag gaactggcag
      541 tattgccatt ggtgggatag gggtatcttt accgatcacc tttattttaa tgggatttac
      601 catgcttttt ggtattggcg gtggagccaa tatttcaata caactgggaa aaaaagacca
      661 gcccatggca agaaaaatac ttgccaacgc acttataatg ctttttttga gcgccttatt
      721 cttaaatggc tttatgctat taaatctaga aaaggttctg cttatttttg gcgcctcagc
      781 gcagaacctt ccttatgcct ctagttactt gcgcattatt cttattggaa actattggaa
      841 tacttttgcc tttgccttta atcatttgat tcgcgcagaa ggcaatccga aattcgccat
      901 gcaaagtatg cttattggag ccatcaccaa tattatcttg gatccaatat ttatttttgt
      961 tttggatatg gggatcgctg gtgcggccta tgcaacaatt attgctcagt ttttaagctt
     1021 cctacgagga tttagctatt atcttggtgg caagaatatt gttaatttaa aaaaggaaga
     1081 ctttcgtttg gaatcaaaag tcatttatat gattgtagca ataggaatat ctcctttttt
     1141 cattcaaata gctggtagtg tagtaggcgc acttcttaat aatagtcttc gaatttatgg
     1201 aggggcccta gctcaaggag cctacgcaat tattaactcc attgccatgc tcttttttat
     1261 gcctattttt ggtatgaacc aaggtctgca acccattgtg ggttttaact atggggcagg
     1321 aaatttcaaa agagtcaaag aagcagtagc tgttggtatt atatcggctt ctactgttat
     1381 gggcctggga tggattgttg tccagttttt ccctcatgta ttagtagggg ttatgacgcc
     1441 agatccagag ctttttgata ttacagttaa gggtcttcgt atcttcttat ctatgatgtt
     1501 tgttgcaggt tttcaaatta taagttctaa ttttttccaa tccattggaa aggcaaagat
     1561 aagctttgtt ctttccttat ctcggcaggt ttttcttctt gcgcccctag tctattttct
     1621 tcctagattt tttggactta agggagtttg gtatgctcaa ccaatagcag atgctctatc
     1681 ttctcttatt actttgtttt ttatcctaag ggagtttaga agtttggaat gggctcactg
     1741 ccagagcaac cctcttgctg agggatgcga agagaatctg gaagttctta gggccatcaa
     1801 agtacaagaa aagacataag agatattaag cctattagcc agatgcttct aggctttttt
     1861 attctataat aaaagtttta taaggctaag tatgctatta ttacaattag attgtgcttt
     1921 ttgaaatgag gataatgtat gtttggtaaa aaaccttcct tggatgcgtc tacccacgcc
     1981 atccatcgta aggaagttat taagatagcc tggcctgtgc ttgttgagct agtcttggct
     2041 agtttattta gtatggtgga tatgatgatg ctcggaaata ttcccagcag aattatagcc
     2101 aaccagtcag tggcagccgt aggaattgtt aaccagcccc tttttattgg gatttcactg
     2161 gttcagtcgg tcaatgtggg atgtacggcc ctggtttctc gctatgtggg ttcaagagaa
     2221 gaacataaga tagaagatac gctaaaacat gtgcttttaa ttagttttat tggtcttgct
     2281 attccttatt ttatccttat gcagatattt agagatccaa ttatgacttt tctgggtgca
     2341 gagccggcgg ttcttgctct tggaggaagt tattttcgaa ttctaattag cggattttta
     2401 ttccaggctt tgaacttttc tcttaccgca gccttacgag gggcggggga gaccaaggtg
     2461 ccaatgaagg tgaatatttt tgccaacctg ctcaatgtat ttggaaatgc catacttatt
     2521 tttggcctac tgggctttcc tgctcttggt ataacaggag cggcaatatc aactgctctt
     2581 tccaacctac tagcctttat cttgatgtat agttatgtga ttgcaggaaa aagcaaggta
     2641 aaacttagcc ttggaaggtt tactctacaa actagcgtat tcctaaacct tgctagaatt
     2701 ggtgtaccag cttcaatgga acaatttgtc cttagagtag gtattatgtt gtttacaaag
     2761 atagttgctg gacttggaac cgcaattttt gcggcacatc aaatagccat tagtatcttg
     2821 ggactgagct ttaacccagg aatggcattt ggcattgctg cctcggcact tgtgggtcgg
     2881 gccctggggg aagaggatgc agaactggcg gcctcctatg ctaatgatgc tagaaagctt
     2941 ggctctaggg tagcaggtgt tgttggaatt ttattgtttg tttttgcccc acaactagtt
     3001 tctttatata ataaggatcc tcttgttata aaaaacgcct caatagcctt aaggattatt
     3061 gcggcaattc agccaataca gtcctctcag cttattttag caggcgctct tagaggggct
     3121 ggggatactt tttccacctt cctagcaacc cttataagcc ttcttgtcat ccgggtaagt
     3181 cttgcctatt tatttgttca tgtgctcttt tatggaatag ctggcgcctg ggcggcagtg
     3241 gtggtagatc aatttatacg ctggatgata gtcaggaccc gctttaaacg tggcaggtgg
     3301 aaaaccctta cattccatag ttaaaagaat tattagagga aggctcaact acttagttgg
     3361 gcttttttat tactgggact ccctgctgct gttttgtttt tggtgtgact tataaaagcc
     3421 ctgtaaagtc gaccttatca atggattttg tttgatttta accccttgga ttggcataat
     3481 tttattgagg taattatgaa tgcaaaaaaa tcaataaaag tggctttttg ggacttatta
     3541 caagaactcc ctctagatca aattacagta atcatgctct gtgaaagggc caatgtaaat
     3601 aggcaaagct tttattatta ctatcagaat attgttgatt tattaaagga tgtgctcttt
     3661 gcagaaatct atgatgaggt ttcccagggc agagcttatg atacctggaa atatggcttt
     3721 tatacgacaa ctcgttttat taatgaaaac cattttatct ttcaaaatat ccaacattct
     3781 aactattggg aagaaatcaa caacttcttt acggaaaact ccaattccct tgttaaagga
     3841 gtcattgacg aatgtattga ggcaacggga atacgtgttt ctaaagaaga taaagatttt
     3901 atgattcact attatagagt tctttttaac agtattatga ctgagtgggt caaggatgga
     3961 atgaaactaa aacccataga acttctctct aaagtagaaa agcttgtgga tgggaccatt
     4021 tgtcctgccc tgcagcgctt ttccgaggaa gaaaactaga caattataag aagttgtcta
     4081 aagactgcaa ccctcccctg aactacaatg aaagcaagag gaaaaagggg agctagcatg
     4141 gttattattc ttgtgcagct aaatcaatca cctctttccc caggggaata aggctagttg
     4201 actagctgtt ttttgttttt aattagcttt gtgagttgag gagggacttt tactatagat
     4261 aaagggtttt ccacttgcta gcagagggtg gtttggcatt ttttgttttc ctatcttttg
     4321 ttagaagtgg agtagcaagc ggtcctataa cttataagaa gcagctctta agagcaaacc
     4381 gttaacaaag tggcattaaa aaaatgccca agctagaaag tagaggagaa agaatgtcta
     4441 aaaaaatacc gtatactgtt gattacagcc ctaagagaag aagtcctata ttacttgatt
     4501 tcgccaatct aattaatagg acaaaaacag gagataaggg tgcactcacc tatgaagatc
     4561 ctgagtatta tgttatggaa aacattgtta ctgatcaaat ggccaaggta ggcttgggtc
     4621 tgaggcttcg cgtatatcaa acagcagagc agattgcaaa gactgtccag atgcctgttg
     4681 aggtagttag agatagacta gaagatcttg ctatggcagg tgcttctcat agagcaacaa
     4741 tagatggtga agttaaatac tggcttgaaa tttgggtacc cggtcatatg gaaatgatta
     4801 tgaataatcg cgaaaacgcg aaaaaatatc cagaaattgc cattggcttt gaggcctatg
     4861 ggaagaaaaa aggaattatt gccccgggat catttcctat gggtatggct cctatgcgtg
     4921 ttatcccgat tgagtctgcc attgatggca actcaagaac tgcaagttat gaagaaattt
     4981 caacctatct taataagcac actatttttt ctgtttccga ttgttcttgt agaaccgttc
     5041 gcgaggaaat gggcgaaggt tgtggccacc taaaagaaga tatgtgtatc cagatgggaa
     5101 cagcagcaga ttattatata catacaggaa gagccagaca gattaccaga gaagaagcct
     5161 ttgaaattat tcataaggca gaagaaaatg gacttctcca ccaaattcca aacctagatg
     5221 gtagtggaga gacccatgct atttgtaact gctgtggctg tggttgtctg gcactacgta
     5281 atgccaatat gttccgcaac cctgattttt cacgctctaa ctatgttgct aaagtcgatt
     5341 cagaaaagtg cgttgcctgt ggagagtgtg tagagcactg cccagttaat gctctaaaat
     5401 taggacaaaa gattcctgcc actgttgccc ttgctgaggt ggacaaacac gaagatcgtc
     5461 ctggaaatac caaatgggga atagataaat ggaatataga ttatcgtgaa aacagagaag
     5521 tggttatgga ctcaggtact agcccttgca aatccttctg tcctgctcat attggtgtac
     5581 agggatatat ccagcttgct tctgaaggaa agtacggtga agctcttgag ctgataaaac
     5641 aagaaaaccc cttcccagca gtctgtggtc atatttgccc acgctactgt gaagatgctt
     5701 gtacacgaaa tagtattgat gactctgttg caattgatga tattaaacgc tttatcgctg
     5761 agcaggatat gaatagcgag caccgctatg tacctaagaa aaagcatgac tatagtgata
     5821 aaaaagtagc ggtagttggc gctggtcctg cgggacttag ctgtgcctac tatctagcaa
     5881 ttgataatta tgatgtaact gtttttgaaa aagaagaagc tcttggtggt atgttaacct
     5941 ggggaattcc ttctttccgc ttggaaaaag aggttattaa ctcagaaatt gatgttctaa
     6001 aagaactggg agttaaattc caaacaggcg tagaaatcgg taaagatctt tccttagatg
     6061 acctgcgcca gcaagactat aaggccttct acctggccat tggagcccag ggtggaagaa
     6121 aactaggtat cgaaggtgaa gatgcccagg gcgtttatag tggagtagat ttcctaagaa
     6181 aggtaaatct tactgaggag cgcttattat caggaaacac cgtagttatc ggtggaggaa
     6241 atgtagctat tgatgttgct cgtacagcag tacgctcagg ttctaaacaa gtacaaatgg
     6301 tttgcctaga aagccctgaa gaaatgccag cacttccaga agaaattcta gaagcccaag
     6361 aagaaggcat tgaagtcctt aacggatggg ggccaaagcg cattctaaca aaagatggta
     6421 aagtcactgg gattgagttt aagagatgta caagagtttt tgatgaaaac cagagattta
     6481 gtcctcttta taatgaagaa gagacctact ttcttgaggc tgataatatt ttagtatcag
     6541 taggccaaag cattgtttgg ggagatcttc taaagggaag taaggcagaa gtaaatccca
     6601 atcagactct acccgccgat ggaacgactt tccaaacagc ccaggaagat atctttgttg
     6661 ggggcgacgc tcatacagga ccaaaatttg ccattgatgc catcgctcaa ggtaaagaag
     6721 gagcaatctc tatccaccgc tttgtgcagc atggtcagag tttagttctt ggaagactaa
     6781 gaagaaatta tcttgccctg gatacggaaa ccgtagacta tcaaggcttt gatttaaaac
     6841 caagacaaga agcagaaaaa gtggatggaa gtgtttctag aacgagcttc cgagatttaa
     6901 gtaagagttt cacgccagaa caaattcaac aagaagctga tcgctgccta agttgcggta
     6961 aaacctatgt tgatgagcat atgtgtgtag gttgtggaca gtgtacaacc aattgtcgct
     7021 ttgatgccat tagtctagaa aaagtttatg atgcagaggg catggaactt agtaaggtca
     7081 aaccctatgt tgtaagaaac gtactaaaga ccaagagcaa aatatttgta cacagtgcta
     7141 aaaagaaaat aatgggaaag taggtttcta gtgcacgaac taggtattat gatagaagtt
     7201 gttaaacagg tcgaagaggt tgctagagaa aatcaagcag agaaagtcga aaagattgtt
     7261 ttacagatag gagaaatatc caccgtaatc ccccattatg tagaagaagt ctttccggca
     7321 gccgtcaata aaaccatgct ggaggatacg cagctggaaa ttgaaattat ccccgccaat
     7381 ggacgctgtt tagaatgtgg aaaggttttt tcccttgtaa gtcaaaaggg aaaatgcact
     7441 tgtgggagtg aagactttga ggaacttaca ggaagagaat tcattttaaa agaaatacag
     7501 attagttaag gagcaaagat gagtgcaatt cgtgttattg aaataaaaaa aggtattttt
     7561 gagcagaatg ataaaaaagc agaaaaaata cgcaaagaat taaaagataa ggggctcttt
     7621 cttcttaatc ttatgagttc tcctggctct gggaaaacca ccacccttgt aagtaccatt
     7681 gagcaactaa aagatgaaat ggagattggt gttattgaag cagacatcga ttctgatgtg
     7741 gatgccagaa ccattgataa gacaggagta agggtcattc agctccatac aggaggcatg
     7801 tgccatctgg atgctgagat gaccagacaa ggcatagaag cgctgggagc tgagggccta
     7861 gactttatgg ttcttgaaaa cattggaaac ctagtctgcc cagcaggctt tgatacggga
     7921 gctacaaaaa acgccatgat tattagcatc cctgagggtc atgataagcc cctaaaatac
     7981 cctgagatgt tcaaggtggt ggatgtggtt cttgtgaaca agattgacgc tatggaatat
     8041 ttcgattttg atttggacct ctttagggac tttgttcata aaatcaatcc tgagattgag
     8101 attattccca tgtcagcaaa aacaggcgag ggtatagagc cttgggttga ttggatccgt
     8161 aagcaggtag ccaagcaaaa agtttaagaa aataccaata atgaaaaaag ggatgctgag
     8221 gtgtcccttt ttttgcgtct tcagataggg caaaagcagt ataaatacag agaaaataaa
     8281 aagaggggaa gagagattat ttggaacaag agggcaataa aaagttaaac agcctataat
     8341 tacatgatat ggccttttaa aaaagccaaa gacaagctat ttataaaaaa cgtcacataa
     8401 tcagacccct tgaaaatgaa aagagggaag atatggccta aattcacata aatatgtaaa
     8461 caacaggctt catagggcaa gttattatga gaaaaacata aacacctaaa aagattagcg
     8521 gaatatagct agggtattta atcaatcacg gctcataaat aaaaaaag
//
LOCUS       DASRNA010000036         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_980258,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000036 DASRNA010000000
VERSION     DASRNA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980258"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="VFD08_01090"
     CDS             complement(<1..1629)
                     /locus_tag="VFD08_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HZK09639.1"
                     /translation="MKKLRKILLLILLLPLLGAALSPVDLNLNGKFVEETPLLEKQGE
                     LFIPIEPFSKLTSLQFREKNKGDIILFRDNVFIKFNTNSSTYFLNGKEFTWKNPPFVE
                     NDELYLPYRILLDFMNFQYSYNAQEKLLELATGTGYDVSLSYRQNRQKVDFAEAKISY
                     NLPYFWKRTSLNSFSSLENSLKIEVSSRPLGEEKPADAIKRLVEEKSFDDFEKRTPRQ
                     LFVEATSIANYGYVKKEQDLYYYYGISYFTLENRLIETTFCAQGATLEPILKLEEDIL
                     SSAQFNAYTIDDMQEHYVELEAFTSLKMSLDDPLYANMLVDNTLAFKGEINPSIKELH
                     ATVKRGDRRFYHSFAVEEGKFDTSIPIPFGLGFHSLSIALSPPPEEETRASSLPYFED
                     KNILLRFSLLNTSLDEGLYLSHSNLVPTQCEEIGILSYQVRNKSFDYYKAEALLSLLK
                     ENFSLGKVDDPLLALEEKTLSKKSAALIYAALLRRAGVPTQVISNISQTIYGVEILSN
                     GRWIVIDPYRFLLNEDPPLDHMSLDQDHFGTRAYY"
     gene            1698..>2465
                     /locus_tag="VFD08_01095"
     CDS             1698..>2465
                     /locus_tag="VFD08_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HZK09640.1"
                     /translation="MDLNKYTLVNLQKKAKELGLSGYSGLRKADLIALVSKKIADQAE
                     ERKKASSQNKAKNQIPKEDIDLVHGYLEIIENKDFGFLRFDNFSPSEEDVYVSPVFIR
                     RFNLKTGDKIQGRVKKNDHSDRYRALIYLDEVNGCKGERAVERKNFSQLTPIHPRERL
                     VLSYNQIELSTRLIDLIAPVGRGQRGLIVAPPKAGKTIFLQNVAKSIEKNYPEVEIIV
                     LLIDERPEEVTEMKRSIKSDVIYSTFDEPPSNHIRVAQ"
BASE COUNT          745 a          488 c          529 g          703 t
ORIGIN      
        1 gtagtaggcc ctggtcccaa aatggtcctg gtctagggac atatggtcta gggggggatc
       61 ttcatttaag agaaaacggt aggggtcaat aacaatccac cgaccattag aaagaatttc
      121 caccccataa atggtttgac ttatattgga gataacttgg gttggcacac ctgcccgcct
      181 aagaagggct gcgtaaataa gagccgcgct ttttttactt agagtttttt cttctagggc
      241 cagaagggga tcatccacct ttcccaggct aaagttttcc ttaagcaggc taagaagggc
      301 ctcggcctta tagtagtcaa agctcttatt tcttacctga taacttaaga tccctatttc
      361 ttcgcactgg gtggggacaa gatttgagtg gcttaaatag agcccctcat ccaggctggt
      421 atttagtagg gaaaacctta gaaggatatt cttatcctca aagtagggaa gggagctggc
      481 ccgagtttct tcctcaggag gcggactaag agcaatactt agactgtgaa aacccaggcc
      541 aaagggaatg ggaatgcttg tatcaaattt tccctcctcc acagcaaagc tgtggtaaaa
      601 gcggcgatca ccccttttta ctgtagcgtg gagttctttg atagaaggat tgatttcccc
      661 tttaaaagca agagtattat ctaccagcat attggcataa aggggatcat ccagggacat
      721 ttttagggaa gtaaaggcct ctagctccac atagtgctcc tgcatatcgt ctatggtata
      781 ggcgttaaac tgggcacttg agaggatatc ttcttctagc tttaagatgg gctcaagagt
      841 cgccccctgg gcgcaaaagg ttgtttcaat taacctgttt tctagggtaa aataacttat
      901 accataataa taatacaaat cctgttcttt ttttacgtag ccataattag caatcgatgt
      961 agcctcaaca aagagctggc ggggcgttct tttttcaaaa tcatcaaagg atttttcttc
     1021 cactagcctt ttaatggcat ctgctggttt ttcttccccc aggggcctac tgcttacttc
     1081 gatctttagg gagttttcca gggaagaaaa actattaaga gaagtcctct tccaaaagta
     1141 ggggaggtta taggatattt ttgcttcggc aaaatcaacc ttctggcgat tttggcggta
     1201 acttaggctg acatcatagc cagtgcccgt ggcaagttcc agtagctttt cctgggcatt
     1261 atagctatat tgaaagttca taaaatcaag caaaatccta taaggaagat agagctcatc
     1321 attttctaca aagggaggat ttttccaggt aaattcttta ccgttaagaa aatatgtgct
     1381 agaattagta ttgaatttaa tgaaaacatt atcgcgaaaa agtattatat ctcccttgtt
     1441 tttttctcta aactgcaggg atgttagttt agaaaagggt tctattggaa taaaaagctc
     1501 cccttgcttt tctaaaagag gggtttcttc gacaaatttc ccattaagat ttaaatcaac
     1561 aggtgatagg gctgctccta gtaaaggaag cagtaaaatt aataacaata tttttcttaa
     1621 ttttttcatg ccaaccagct ttcttctctt ctcatatcat atctaatttt cgccacctat
     1681 gcaatcggag gtttattatg gatttaaata aatatacact agtcaactta caaaaaaaag
     1741 ccaaagaact tggccttagt ggctatagtg gcctgcgtaa ggccgattta attgccctag
     1801 ttagtaaaaa aatagcagac caggcagaag agaggaaaaa agcaagtagc cagaataagg
     1861 caaaaaacca aatcccaaaa gaagatattg atcttgtcca tggctaccta gaaattatag
     1921 aaaataagga ctttggtttt ttgcgttttg ataatttttc tcccagtgaa gaggatgtat
     1981 atgtatctcc tgtatttatc cgccgcttta atttaaaaac cggggacaaa attcaaggac
     2041 gtgtaaaaaa gaatgatcat tcagaccgct accgggccct aatctaccta gatgaagtaa
     2101 atggatgtaa gggagaaagg gcagtggaaa gaaaaaactt ttctcaactg acccccatcc
     2161 atcccagaga gcgcctggtt cttagctaca atcaaataga actatccacc cggctgattg
     2221 atttgattgc tccagtgggc cggggccaga gggggcttat tgtggctccc cccaaggcag
     2281 gaaaaaccat cttcctccaa aatgtggcca agagcattga aaaaaactat ccagaagtgg
     2341 agattattgt gcttttaatt gatgaaaggc ccgaagaggt cacagagatg aaaagaagca
     2401 ttaaaagcga tgtcatctac tcgacctttg atgagccccc ttctaatcat attagggtgg
     2461 cccag
//
LOCUS       DASRNA010000037         7988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_158164,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000037 DASRNA010000000
VERSION     DASRNA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158164"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFD08_01100"
     CDS             <1..792
                     /locus_tag="VFD08_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZK09641.1"
                     /translation="DMNLRSNFGVNLLGVARVGERVAESPNRIEIRGGDVLLLQGAEG
                     SIQEVIRELGALPLVNRGIELKSNTKILLSLSIFIGALVLVALELVIVEIAFVSAALI
                     MVGTKVISIKRAYEAMDLPILMLLGSLIPVSNALETTGAASMLSRLLLSITPGVDPWV
                     SLLMLMVLTMVLTNIINNAAAALIMAPVALGAAQLLSLNPDPFLLVVFVGATSAFMTP
                     IGHQSNTIIMGPGGYHFGDYWRLGLLVSALTCLVSLPVILYFWPV"
     gene            803..1414
                     /locus_tag="VFD08_01105"
     CDS             803..1414
                     /locus_tag="VFD08_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09642.1"
                     /translation="MIKCSKTNVYNFENALRGMRNPLDSWSMSDSSFEGEVVLGEKDL
                     RLAKQLRKAGSDHRKYLRQILVSVDIEGPLYWWKEFDTYKVATVANSTSTMHTIHKKG
                     LVPEMFSVEGISKQTQEVFASYLAYLEGLRLSFNKTKDKKTWEEIIKLLPSSFNQLRT
                     VTLNYETLMSIYQARKNHKLVEWREFCQWIEKLPYFKEIVLED"
     gene            complement(1486..2217)
                     /locus_tag="VFD08_01110"
     CDS             complement(1486..2217)
                     /locus_tag="VFD08_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK09643.1"
                     /translation="MKHLIYFSPCGGTKKYLEKLMASKEETKVRDLTYKAQERLSLSK
                     EDLLIIAFPVYYGRVPLLFLERLKNLEGSKTPAIILAVYGNRAFDDSLLEIKDFLIKK
                     GFIPYAGAGLIAKHSFGLIQTHRPHKKDLEEVNDFLIQAIKKKSRAKAYNFPGNFPYK
                     ERGSLSFFPSTTDDCILCKACLRACPAHAIDEKINTLSERCILCMACLDVCPMGARVL
                     DQEFDDFIKAFSEKLKEPRKNEFFL"
     gene            2332..3324
                     /locus_tag="VFD08_01115"
     CDS             2332..3324
                     /locus_tag="VFD08_01115"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HZK09644.1"
                     /translation="MKNKKLNLANLPTKIEKMPAISKKLGLNIYLKRDDQTGSEISGN
                     KIRKLEYALYEALEQGADTLITCGGIQSNHCRATAAAGAKLGLKTILYLRTPVRQPVE
                     GNYFLDQLLGAEVRFITPKEYNYHRQEIMEEAAQEVQRQGKKAYLIPEGASNGIGTLG
                     YYEAFLEILAQEKQMGVEFDTVVCAVGSAGTYSGLHFANILGEHGKKVVGIPIAADSQ
                     HFAGRTREIWQEFSKIIGSDKNLENEKMYLLDGYAGRGYALNTPEEMDFVLQISRSEG
                     VIFDSVYTGKAFRGLVDALEKEHSYFENSKNILFIHTGGLFGLFDKKFEFDFHQ"
     gene            3452..5230
                     /gene="glgB"
                     /locus_tag="VFD08_01120"
     CDS             3452..5230
                     /gene="glgB"
                     /locus_tag="VFD08_01120"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZK09645.1"
                     /translation="MNLLPALEDLRAYREGTFYEGAEHFGAQYIQDQFRFLVYAPRAK
                     EVSVVGEWNQWTPGVDPMKPIEEGYYYAFIPEAREGHLYKYHILDQEGKEIWKTDPYA
                     RKIRWGQEGAGIIYRSKDFDWQAKIDIKENLTIYEVHLGSWKKKEDGTYLSFQELAHS
                     LLPYVEDLGYKAIELLPVTEHPYDGSWGYQQTGYFAITSRHGDPWDFKYFVEEAHKRG
                     LAVFLDWVPCHFAKDAPGLSYFDGSALYESDFWEDAYNSQWDTINFDFGRRHVCNFLI
                     GNLFFLWKEYGIDGIRVDAVAHMLYREEGLKNIHDHSLNFLQELTTALKGKILLFAED
                     SSDYEGITKAVSKGGLGFDYKWNMGWMNDTLRYIGLNGYAKTHKHNLLTFPMVYAFSE
                     RFVLPLSHDEVVHMKGSLLTKTQGTYEEKFSQLRLLHLYQYAMPGKKLLFMGGEFGQF
                     AEWNAFTGLDWNLLDFYSHLGVQTFVRDLNLLYHKKPALYENDQDWKGFNWIEVDNPQ
                     QNFIAFKRIDLKGNEVMVLLNFSSKELFNHKLDIKAGKYRILINSDAEEYGGKARGSK
                     SPVMGESEIYIGLAPYSGMILEVIND"
     gene            5223..6359
                     /locus_tag="VFD08_01125"
     CDS             5223..6359
                     /locus_tag="VFD08_01125"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZK09646.1"
                     /translation="MIREKMVAMLLAGGQGTRLKQLTEKNAKPAVHFGGKYRIIDFVL
                     SNCSNSGIRSVGILTQYQPLALSMHIGIGGPWDLNRHRGGVEILPPHMTTEGGRWYAG
                     TANAIYENLDYIALQDPDHVLILSGDHIYKMDYEKMLKFHVEKGAAVTISAIKVPLEE
                     ASRFGMLNIEEDGKIYEFEEKPEKPKSDLASMGVYIFNWDMLHTWLRKDHANPDSEHD
                     FGKDVLPGMLEEGLALYAYQFDGYWKDVGTVESYWQSNMDLLDPENKLNLFDSEWRIY
                     TKNYDVPPQYLSNTSKVERALINEGCIVHGSVKNSILSTSVVVEEGAQVKDSILLPGA
                     HIGKNAILNRVIVLSHETVEANAKIGSENGEIEIVGFVDHGLVV"
     gene            6368..7486
                     /gene="glgD"
                     /locus_tag="VFD08_01130"
     CDS             6368..7486
                     /gene="glgD"
                     /locus_tag="VFD08_01130"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HZK09647.1"
                     /translation="MKTKVAGLINLGERETYIKELTRYRPLATVPFAGRYCLIDFMLS
                     SMVNSGIENVSILVKDKFHSLNDHLSFGKWWDLDRKTGGIRVLYPDIKEEMAYEGDIQ
                     RLRSNLIHFEKTRAQHILLTRSNYLGNIDFNEAFDYHEENDADITVLTKIVKDGKTRV
                     DLLGLDILMKENDRIYIGRNLGNQQEFELGIEMYFIKKEVFMEIVREALEEGKESSLK
                     GAIQRRLKKYKVLTYGLEEEILPIYSMQQYYKASLRILEEDKAHELFYKNGKIFTKTK
                     DAPSTLYMEGSEVKNSLIANGCVIKGTVENSILFRNVEVGENAVIRNSIVFQGSKVGN
                     GAQINFAVLDKRSSIGKGRILMGDGGLPYFVGKGVKIL"
     gene            7483..>7988
                     /locus_tag="VFD08_01135"
     CDS             7483..>7988
                     /locus_tag="VFD08_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HZK09648.1"
                     /translation="MKILFAASESSPYIKSGGLADVLHSLPKELARRGNDVRVVIPLY
                     KHSSRKVQEEFNYLGSFHVEMPTSSEYAGVFSHKNDGVIYYFIDNEHYFYRENLYGQY
                     DDGERFSFFNHAILKLMEEIGFYPDILHLNDWHVGAAAVLFKDGYSWREEFKKTKVVF
                     TIHNLKYQG"
BASE COUNT         2454 a         1413 c         1842 g         2279 t
ORIGIN      
        1 gatatgaatt taagaagtaa ttttggagtc aatcttcttg gggtagccag ggtgggtgaa
       61 agagtagcag aaagtcccaa ccgaatagaa atccggggag gagacgttct tcttctgcag
      121 ggggcagaag gaagcattca ggaagtaata cgagaactgg gggcccttcc cctggtaaac
      181 cggggcattg aacttaagag caatactaag attctcctca gtctttcaat ctttattgga
      241 gccttggtcc tagtggccct agagcttgtg attgtggaaa tagcctttgt atctgctgcc
      301 ctaattatgg tgggcaccaa ggttattagc attaaaagag cctatgaagc catggatcta
      361 cccatcctta tgcttcttgg cagcctaatt cctgttagta atgctcttga aactaccggc
      421 gctgccagta tgctctcaag gctcctgcta agtattactc ctggggtaga tccctgggtg
      481 agtctactaa tgcttatggt tttgactatg gtactaacca acattatcaa taatgcagca
      541 gcggccctga ttatggcgcc tgtcgccctg ggggctgccc agctcttgtc gcttaatccg
      601 gatccctttt tactggttgt ttttgtaggg gccacaagtg cctttatgac ccccattggc
      661 caccagtcca atacgattat tatgggcccg ggaggatatc attttggaga ttattggcgt
      721 cttggtcttc tggtaagtgc cctaacttgt cttgttagtt tacctgttat tttatatttt
      781 tggcctgtat aaggaggaag ttatgattaa atgtagtaaa accaatgtgt ataattttga
      841 aaatgcccta agaggtatga gaaatccctt ggatagctgg tctatgtcag acagtagttt
      901 tgagggggaa gtagtgctag gagaaaaaga tcttcgtcta gccaagcagc taagaaaagc
      961 tggcagtgat caccgcaagt atcttcgcca aattttagtt agtgttgata ttgagggacc
     1021 tctttattgg tggaaagaat ttgacaccta taaggtagcc actgtggcca attctaccag
     1081 cacaatgcat accattcata aaaaaggtct tgtccctgaa atgtttagtg tagaaggaat
     1141 aagtaagcag acccaggagg tctttgccag ctatcttgct tatcttgagg gtttgcgtct
     1201 ttcttttaat aaaacaaaag acaaaaaaac ttgggaagaa attattaaac ttcttcccag
     1261 cagctttaat caactaagga cagtaaccct aaattatgaa actcttatga gtatctatca
     1321 ggcccgaaaa aaccataaac tagtagagtg gcgagagttt tgtcagtgga tagaaaagct
     1381 accgtatttt aaggaaattg ttttagaaga ctagtagaaa ctagggcttt cttaagtaga
     1441 ggcaaaataa atattttagg cagcaagagc tgtgtgatag aggagctaaa gaaagaactc
     1501 attttttctt ggctccttta atttttcgct aaaggctttt ataaagtcat caaactcttg
     1561 gtcaaggact cttgccccca taggacagac atcaagacag gccatacata ggatgcagcg
     1621 ctcactaagg gtatttattt tttcatcaat ggcatgggcg gggcaagctc taagacaagc
     1681 cttacacagg atacaatcat ctgttgtact tggaaaaaaa cttaaacttc ccctttcctt
     1741 ataggggaaa tttcccggaa agttataggc ttttgccctg cttttctttt ttatggcctg
     1801 gatcagaaag tcatttactt cctctaaatc ttttttgtgg ggtcggtggg tctggataag
     1861 accaaaacta tgtttggcaa ttaggccagc gccagcatag gggataaagc ctttttttat
     1921 aagaaaatct tttatttcta aaagagaatc atcaaaggct ctattgccat aaacggccaa
     1981 gataatggcg ggggtcttac tgccctcaag atttttaagg cgctcaagaa aaagaagggg
     2041 gaccctgcca tagtaaacgg gaaaggcaat gataagaagg tcttccttgc ttaggcttag
     2101 cctctcttgg gccttatatg taagatcccg tacttttgtc tcttcttttg acgccatgag
     2161 tttttctagg tattttttcg ttcctccgca aggagaaaaa taaataagat gtttcattta
     2221 aagctcctct tttattatag tttaccacta gtataaggaa aatactccta gccaaatact
     2281 attaaaaacc cgctttattt gatatagtgt tgttaagaaa ggtaggctaa tatgaagaat
     2341 aaaaaactaa atcttgcaaa tttaccaaca aaaattgaaa agatgccagc gatcagtaaa
     2401 aagcttggtc taaatatata tctaaaaaga gatgatcaaa caggaagtga gatttcggga
     2461 aataaaatca gaaaattaga gtatgccctt tatgaggccc ttgaacaggg ggcagacacc
     2521 cttattacct gtggaggcat ccaatccaat cactgtaggg caactgccgc agcgggagcg
     2581 aaactgggcc taaaaaccat actttattta cgtactcctg tgcgtcagcc tgttgaagga
     2641 aattattttc ttgaccagct cctaggggct gaggtgcgtt ttatcacgcc aaaagaatat
     2701 aattatcatc gccaggaaat aatggaagaa gccgcgcagg aagtccaaag gcagggcaag
     2761 aaggcctatc ttattccaga gggagcttcc aatggcatag ggaccctggg ttactatgag
     2821 gcctttttag aaattttagc acaagaaaag cagatgggag tggaatttga tacagtagtc
     2881 tgtgctgtgg gcagtgcagg cacctactct ggccttcact ttgctaatat tttaggagaa
     2941 catggaaaaa aagtagtggg cattcctatt gcagccgaca gccaacattt tgctggtaga
     3001 acaagggaga tttggcaaga gttttctaag ataataggca gtgataaaaa tctagaaaat
     3061 gaaaaaatgt atctactaga tggctatgcc ggtaggggct acgccctgaa cactccagaa
     3121 gaaatggatt ttgtgctcca aattagccgc agcgaagggg ttatatttga tagtgtatat
     3181 acaggtaagg cctttagagg tctagtggat gcgctagaaa aagaacacag ctattttgaa
     3241 aatagtaaaa atatactctt tatccataca ggcggtcttt ttggcttgtt cgataaaaag
     3301 tttgaatttg attttcatca gtaggctaga cgctccgcct ataaaaaaac ttatagatat
     3361 agaggaagaa cagaaaaaac aaacttgttt ttttaactaa agttaatcat tgtgataaat
     3421 attgggtata ttaaatagaa ggagggaaat aatgaattta ttgcctgctt tagaagattt
     3481 acgcgcctac cgagaaggaa ctttttatga gggtgcggaa cattttggtg ctcagtatat
     3541 tcaggatcaa tttcggtttt tagtctatgc accccgtgct aaggaagtct ctgttgtggg
     3601 tgagtggaac cagtggacac caggggtgga ccctatgaag cccattgaag aggggtatta
     3661 ttacgccttt attccagaag ctagggaagg gcatttatac aaatatcata ttcttgatca
     3721 agaggggaag gaaatttgga agaccgatcc ctatgccagg aagataagat ggggtcaaga
     3781 aggtgctggg attatttatc gctccaagga ttttgattgg caggccaaaa tagacataaa
     3841 agaaaatctg acaatttatg aagtgcactt gggtagttgg aagaaaaaag aagatgggac
     3901 ttatttaagt ttccaggaac tggcccatag ccttcttccc tatgtagagg atttaggcta
     3961 caaggccatt gaactactgc ctgttactga gcacccctat gatggcagct ggggttatca
     4021 gcagacgggt tattttgcta taaccagtcg ccatggggat ccttgggact ttaaatactt
     4081 tgtggaagag gcccataaaa gaggccttgc cgtttttctt gattgggtgc cttgccattt
     4141 tgccaaggac gcccccgggc tttcttattt tgatggaagc gccctttatg aaagtgattt
     4201 ctgggaggac gcctataatt cccagtggga tacaataaac ttcgattttg gcagacgaca
     4261 tgtctgtaat tttcttatag ggaatttgtt ttttctttgg aaggagtatg gcattgatgg
     4321 aattagggtg gatgctgtag ctcatatgct ctatagagaa gaaggcctca aaaatattca
     4381 tgatcattct cttaatttcc tacaggaact aacaacagcc ttaaagggca aaatccttct
     4441 ttttgcagaa gattcttctg actatgaagg aattactaaa gctgttagca aaggtggtct
     4501 gggttttgac tataagtgga atatgggttg gatgaatgat actctgcgct atataggcct
     4561 taatggctac gctaaaactc acaagcacaa tcttttgacc tttcccatgg tctatgcttt
     4621 tagcgaaagg tttgttttgc ccctaagcca tgatgaggtg gtacatatga agggttctct
     4681 tcttacaaaa acccagggaa cctatgaaga aaagttttct caattacgcc ttcttcatct
     4741 ttatcaatac gccatgccag gcaaaaaact tttatttatg ggaggggaat ttggacagtt
     4801 tgccgagtgg aatgcattta caggcttaga ctggaactta ctagactttt actcccatct
     4861 aggagtgcaa acctttgtaa gggacttaaa tctcctctat cataaaaaac cggctctgta
     4921 tgaaaatgac caagattgga agggctttaa ctggattgag gtggataatc cacaacaaaa
     4981 ctttattgcc tttaagcgga ttgatttaaa gggaaatgaa gttatggttt tgttaaactt
     5041 ctcatctaag gaacttttta accacaagct ggacattaag gcaggcaagt accgcatatt
     5101 aattaattct gacgcagaag aatatggtgg aaaagcaagg ggctctaaga gccctgtgat
     5161 gggtgaatcg gagatctata ttggactcgc tccctatagt gggatgattc tggaggttat
     5221 aaatgattag agaaaaaatg gtagccatgc tacttgctgg gggacagggg acaagactaa
     5281 aacaattgac agaaaaaaac gcaaaacctg ctgtgcattt tggaggaaaa taccgaatca
     5341 ttgactttgt acttagtaac tgctccaact caggaataag aagcgtggga atactgaccc
     5401 agtatcagcc cttggccctt agtatgcata ttggcattgg aggtccctgg gacctaaacc
     5461 gccatagagg cggggtggag atcctgccac ctcatatgac caccgaggga ggccgatggt
     5521 atgcgggcac agccaatgct atttatgaaa acctcgatta tattgcccta caagatccag
     5581 atcatgtgct gattctctca ggggatcata tctataagat ggactatgaa aagatgttga
     5641 aatttcatgt agaaaaaggg gccgctgtta ccatcagtgc cattaaagtt cccttagaag
     5701 aggccagtcg atttggcatg ctaaatatag aagaagatgg aaaaatttac gaatttgaag
     5761 aaaaaccaga aaaaccaaaa agtgacctgg cctctatggg agtctatata tttaactggg
     5821 acatgctgca cacctggtta agaaaagacc atgcaaaccc tgactcagaa catgactttg
     5881 gtaaggatgt tctaccaggt atgttagaag aaggcctggc cctttatgcc tatcaatttg
     5941 atggctactg gaaagatgta ggtacagtag aaagttattg gcagtcaaat atggatctat
     6001 tagaccctga aaacaagtta aacttatttg atagtgaatg gcgaatttac acgaaaaact
     6061 acgatgtacc accccagtat ctttctaaca cttcaaaggt agaaagggcc cttattaatg
     6121 agggctgtat tgtacatgga agtgttaaaa acagcattct aagtacttct gtggtagtag
     6181 aagaaggagc tcaagttaaa gacagtatcc tgcttccagg agcccatata ggaaaaaatg
     6241 caatcttaaa ccgagtcatt gttctaagcc atgaaacggt agaggccaat gcaaaaattg
     6301 gaagtgagaa tggggaaatc gaaattgtag gttttgtaga tcacggactg gtagtttagg
     6361 aggaaccatg aaaacaaagg tagcaggttt aattaattta ggagaaagag aaacctatat
     6421 taaggagctc acaaggtaca gacctctggc aacggttcct tttgctggac gttattgttt
     6481 aattgatttt atgctatcaa gcatggtaaa tagtggcatt gaaaatgtgt ctattttagt
     6541 aaaagacaaa ttccactcct tgaatgacca tttgagtttt ggcaagtggt gggacttgga
     6601 ccgaaagacc ggtgggatcc gggtacttta tcctgatata aaagaagaaa tggcatatga
     6661 gggagatatt caaagattgc ggagcaactt gattcatttt gaaaaaacca gggcccagca
     6721 tattctttta actaggagta actatcttgg aaatatcgac tttaatgaag cctttgacta
     6781 tcatgaagaa aatgatgccg atattaccgt tcttacaaaa atagtaaagg atggaaagac
     6841 ccgggttgat cttttaggcc ttgatatcct aatgaaagaa aatgatagaa tttatattgg
     6901 gagaaatctt ggcaatcaac aggagtttga acttggtatt gagatgtatt ttattaaaaa
     6961 agaagtgttt atggagattg taagagaggc cctagaagaa ggcaaagagt cttctctaaa
     7021 aggagccatc cagcgtaggc taaaaaaata caaggtatta acctatggcc tagaagaaga
     7081 gattcttcct atttactcca tgcagcagta ttataaggcc tcccttcgca tccttgaaga
     7141 ggacaaggcc catgagctct tttataaaaa cggaaagatt tttaccaaga caaaggatgc
     7201 tcccagcacc ttatatatgg aaggaagtga agttaaaaac tccctaatag ccaatggttg
     7261 tgttattaaa ggaacggtag aaaactctat cttgtttaga aatgttgagg tgggagaaaa
     7321 tgccgttata cgaaactcca ttgttttcca aggatcaaag gtaggtaatg gggcccaaat
     7381 taactttgcg gttttagaca agcgcagttc aattggtaag ggtcgcatcc ttatgggaga
     7441 tggtggtttg ccatattttg taggaaaagg agttaaaatt ttatgaaaat tctttttgca
     7501 gccagcgaat cttcacccta tataaagtct ggggggcttg ctgatgtact tcattctttg
     7561 cccaaggaac tagctaggcg aggaaatgat gtgcgggttg ttatccccct ctataagcat
     7621 agtagtagaa aagtccaaga ggagtttaat tacttggggt cttttcatgt agaaatgccc
     7681 acatccagtg agtatgccgg agttttttct cataaaaacg atggggtcat ctactacttt
     7741 attgacaatg aacattattt ttaccgggaa aacctatatg gccaatatga tgatggcgag
     7801 cgctttagct tttttaacca tgcgattttg aaattgatgg aggaaatagg tttttatcct
     7861 gatatcttgc atctaaatga ctggcatgta ggagccgcag cggttctttt taaagacggc
     7921 tactcttggc gggaggaatt taaaaagacc aaggtcgttt ttaccattca taatcttaag
     7981 taccaggg
//
LOCUS       DASRNA010000038         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_158616,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000038 DASRNA010000000
VERSION     DASRNA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158616"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VFD08_01140"
     CDS             <1..1506
                     /locus_tag="VFD08_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HZK09649.1"
                     /translation="TREEDEILIKKICLLAASENAEAKKETLKALRRFVIEENTDLDR
                     SLEAYSFIKSFPAKEGSDMQGSYYQIGDKDFSYVLGSLEEVLEISSFYNLDNDPLPIT
                     EEVRQGIQSTNAQFIEEGKKVFAMACKEADSIETGKLNFLGLIALGDQGRSGAKETIK
                     TLKNAGIRPILLSKSPTEHAFELAKELGILIPGYEVMTSEAMSSLSKEDFENSIENYS
                     VYTNLSLDQRIQLIEGWKNKGASVAITADEIEDTGAFEAADISISASNQKFGVVKQMA
                     QMILPEDNLGALTEAIANARMVYYNLRRKVRFELSISLGLILSMVSIMLLFNDFAFSY
                     LALLWLGFVVSAFPSLALEKEVKERNTMVQAPPELDKGLITRRSFVLTMIESILIAAV
                     SVGGYYALSASAQELARTLTYSTFGFAILFQAFSLRNSQSVLGEGFFSNKSFFIAFML
                     AGALLLIPTLVPYVNTIFGMTNLPVQYWGLALGLGFSPMIVTEIRKIFIKE"
     gene            1709..2731
                     /gene="hrcA"
                     /locus_tag="VFD08_01145"
     CDS             1709..2731
                     /gene="hrcA"
                     /locus_tag="VFD08_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZK09650.1"
                     /translation="MLTSRQIEILKLIVDDFILTGSPVGSRRLSKIPHLKVSSATIRN
                     EMADLEDYGYVIQPHISAGRIPSELGLRFYVDSLLSDEELPASTNSQIRSLYFMQYEE
                     SSNLLEQAARVLSHISEMTVLITVPEFSARRLTNMKLVKMSPSKVLLVLITDTEDVKT
                     LILNSDDVSQKELDALNVEFSNLVLQHSLDEISLKDFTKIKSNHIHLSKLIDYILPAL
                     RDALSKLNKKNIIVSGRENLLRPGYFTDMDLARKALGMLKDPIAVLNIFDNSTRDLTV
                     RIGAELGNETFSEFSFVQANYGYGGGQSGQIGLLGPKRMDYSKVISIVEVGAKTLTDL
                     FSGIHL"
     gene            2742..3284
                     /gene="grpE"
                     /locus_tag="VFD08_01150"
     CDS             2742..3284
                     /gene="grpE"
                     /locus_tag="VFD08_01150"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZK09651.1"
                     /translation="MDKEEVKIVDEKKELVGASDKTEEISAEETPEEEGLELEDELDR
                     LKDRHLRLQAEYANYRRRTEEEKSKIYSFANEKLILELLPIIDNFARALDNMKENDVS
                     KSYKDGVKMIEKSLMQVLEKQGLECIEACGKEFNPEFHHAVLTEESKEKNSGIILEEL
                     QKGYKLKDKTIRPTMVKVSK"
     gene            3296..>4960
                     /gene="dnaK"
                     /locus_tag="VFD08_01155"
     CDS             3296..>4960
                     /gene="dnaK"
                     /locus_tag="VFD08_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZK09652.1"
                     /translation="MAKIIGIDLGTTNSCVAVLEGGEAKIIANAEGNRTTPSIAAFTK
                     KGERLVGETAKRQAITNPERTIMSVKREMGSDWNTEIDGKKYSAQEISALILAKLKTD
                     AESYLGEEVKQAVITVPAYFEDAQRQATKDAGRIAGLEVLRIINEPTAAALAYGLDKD
                     HLNQKILVFDLGGGTFDVSILEIGDGVFEVLATNGDNHLGGDDFDKEIINWLVAQFKK
                     EHDVDLTGDLTAMQRLKEASEKAKMELSTVQTTNINIPFLYMSEAGPLHLDINLTRAK
                     FDQLTEKLIKRVEGPMKKAMADSGLKESQIDDVILVGGSTRIPAVVSAVKRIVNKEPH
                     KGVNPDESIAAGAAIQAGVLAGDVGDILLLDVTPLSLGIETLGNVFTKLIERNTTIPT
                     KKSQIFSTATDNQPAVDIHVLQGERPMSTDNVTLGRFQLDGIPPARRGIPQIEVTFDI
                     DSNGIVHVSAKDLGTGKEQSINITASANMSEEEIEKKVREAEEFAQEDKKRQELAEAK
                     NNGDALAYQAEKTIDELGDQIEEEEKTKIEGLISSLKTALENDTLDE"
BASE COUNT         1680 a          806 c         1069 g         1405 t
ORIGIN      
        1 acaagagaag aagatgaaat cttaataaaa aagatttgcc ttcttgccgc ttcagaaaac
       61 gcagaagcta aaaaggaaac cttaaaggcg ctgcgacgtt ttgtgataga agaaaatact
      121 gatttagatc ggtcattgga agcctatagc tttataaaaa gttttcccgc aaaagaaggc
      181 agcgatatgc aggggagcta ttaccaaatt ggagataaag actttagcta tgttttaggt
      241 agcttagaag aagttttaga gatttctagt ttttataatt tggacaatga tcctttaccc
      301 attactgagg aagtccgtca aggaatccaa agtaccaatg ctcaatttat agaagaaggc
      361 aaaaaggttt ttgccatggc ttgtaaggaa gcagattcta tagagacagg aaagttaaat
      421 ttcctaggac ttatagctct aggagatcaa ggccgtagtg gtgcaaaaga gactattaaa
      481 actcttaaaa atgcgggtat tcgtcccatc cttctttcaa aaagcccgac tgagcatgcc
      541 tttgaacttg caaaagaact gggtatctta atcccaggtt atgaagtaat gactagtgaa
      601 gcaatgagca gtttgtccaa ggaagatttt gagaattcca ttgaaaacta ttcagtctat
      661 acgaatttga gcctagatca acgaattcaa ttgattgagg ggtggaagaa caaaggagcc
      721 agcgtagcta taacggcaga tgaaattgaa gatacaggtg cttttgaggc ggcagatatc
      781 tctattagtg cttccaatca aaaattcggt gtggtaaagc agatggcaca aatgatttta
      841 ccagaagata atcttggcgc tcttactgag gcaattgcaa acgcacgtat ggtttattat
      901 aatctaagaa gaaaagttcg ctttgaatta agcatttctt tgggacttat ccttagtatg
      961 gtcagtatta tgcttttgtt taatgacttc gcttttagtt atttggccct attatggctt
     1021 ggttttgttg tgagtgcctt tcccagcctc gctttagaaa aggaagtaaa agagcgcaat
     1081 acgatggtcc aggcaccacc ggagctagat aagggactta taacaagacg tagttttgtc
     1141 ctcactatga ttgaatcaat ccttattgca gcggtcagtg taggaggcta ctatgcttta
     1201 tcagcttcgg ctcaagagtt ggcaagaact ttaacttatt ctacctttgg atttgctata
     1261 ctttttcaag cctttagttt gcgaaatagt cagtcggtac ttggagaagg ttttttctcc
     1321 aataaaagct tttttatagc ctttatgcta gcaggggcac tccttcttat tccaaccctt
     1381 gtgccctatg taaatacaat ctttggtatg acaaatttac ctgtgcaata ttggggactg
     1441 gctcttggcc ttggtttctc tccaatgata gttacagaga tcaggaaaat atttatcaaa
     1501 gaatagtaag ggtattttag gcatcttttg ataggtgcct ttttttattg caaatatgaa
     1561 tgagatgaca agataaagat tctagaataa gccctttgct cttcaaggat tttatattat
     1621 tttgactcta ggtgttgaca agtgaaatgg tttgggtata atagtattgg cactcaaaag
     1681 agatgagtgc taatatggag aaaaaaatat gttaacatca agacaaatag aaattttaaa
     1741 attaatagtg gatgatttta tcctaacggg ttcgcctgtg ggtagtagaa gattgtccaa
     1801 aattcctcat ctaaaagtat cctcagcaac cattcgaaat gaaatggctg accttgaaga
     1861 ttatggatat gttatacaac cccatatatc agcgggcagg ataccatcgg agctaggact
     1921 tcgattttat gtagattcac ttttaagtga tgaagaacta ccggcttcga ctaattctca
     1981 gattcgctcc ttgtatttta tgcaatatga agaaagcagc aatctattag aacaagctgc
     2041 acgagtcttg agccatattt cggaaatgac cgtgctcata actgtgccgg agttttctgc
     2101 tagacgactt accaatatga aactggtgaa aatgagtcct tcaaaggttc ttcttgtctt
     2161 aatcacagat acagaagatg ttaaaacctt aattcttaat tctgatgatg tttctcaaaa
     2221 agagcttgat gcacttaatg ttgaattttc aaatctagta ttacaacact ctcttgatga
     2281 aatatcttta aaggatttta ctaagattaa gagcaatcat attcatttaa gtaagttgat
     2341 tgattacatt ttacctgccc ttagagatgc tttaagcaaa ttaaataaaa agaacattat
     2401 tgtatctgga agagaaaatt tgttacgccc tggatatttt accgatatgg acctggcccg
     2461 aaaggcgctt ggaatgttaa aggatccaat agctgtatta aatattttcg ataacagtac
     2521 aagagatctt actgtgcgaa taggggctga acttggaaat gaaactttta gcgagttttc
     2581 ctttgttcag gctaattatg gttacggtgg aggtcaaagc ggccagattg gcctcttagg
     2641 acccaaacga atggattata gcaaggttat aagcatagta gaagtgggag ctaaaaccct
     2701 gacagatctt ttttcaggga tacatttata ggaggttgtg tgtggacaaa gaagaagtta
     2761 aaattgtaga tgaaaaaaaa gaattagtag gggcaagtga taaaacagaa gaaatatctg
     2821 cagaggagac ccccgaggaa gaaggcttgg agctggaaga tgagctagat aggttaaaag
     2881 atcgacatct tagacttcag gcagaatacg ccaactatcg acgtaggaca gaagaagaga
     2941 agtcaaagat ttatagcttt gcgaatgaaa agttaattct agagcttctg cctattattg
     3001 ataattttgc tagagcactt gataacatga aagaaaatga tgtttctaaa agttataaag
     3061 atggggttaa aatgatagag aaatctttaa tgcaagtgct agaaaagcag ggcttagaat
     3121 gcattgaagc ctgcggtaaa gagtttaatc cagaatttca ccatgctgtg ttaacagaag
     3181 agagcaagga gaaaaattcc ggaattatac tagaagagct tcaaaaagga tataaattaa
     3241 aagataagac gatacgacca actatggtaa aggtatcaaa ataggaggaa aaaaaatggc
     3301 taaaattata ggtattgatt tagggacaac aaactcttgt gtagctgtgc ttgaaggggg
     3361 cgaagcaaaa ataattgcaa atgctgaagg caatagaact acaccatcca ttgcagcttt
     3421 tacaaaaaaa ggcgagagac ttgtaggtga aacagcaaag cgacaagcga ttacaaatcc
     3481 tgagcgcaca attatgagtg ttaaaagaga aatgggctca gactggaata ctgaaattga
     3541 tggaaaaaaa tactcagctc aggaaatttc tgctctgatt ttggcaaaac taaaaacaga
     3601 tgctgaaagc tatttgggag aagaagttaa gcaagccgtt ataactgtac ctgcttactt
     3661 tgaagatgcg caacgtcaag caacaaaaga cgctggtaga attgctggcc ttgaagttct
     3721 tagaatcatc aatgagccta cagcagcagc tcttgcttat ggtttagata aagaccatct
     3781 caatcaaaag atccttgttt ttgacctcgg tggtggaacc tttgatgttt ccattcttga
     3841 aattggagat ggagtttttg aagtgcttgc tacaaatggt gacaatcacc ttggtggcga
     3901 tgactttgat aaagagatta tcaattggct agtagcccag tttaaaaaag aacacgatgt
     3961 tgatttaact ggtgatctta cagctatgca acgactaaaa gaagcatctg aaaaagcaaa
     4021 aatggagtta tcaacggtac aaacaacaaa tattaatatt ccatttctat atatgagtga
     4081 agctggaccc ttgcacctag atattaacct tactagggca aagtttgacc agctaacaga
     4141 aaaactaatc aagcgtgttg aaggcccaat gaaaaaagca atggctgatt ctggtttaaa
     4201 agaaagccaa atcgatgatg ttatccttgt tggtggttca actcgtattc ccgcagttgt
     4261 tagtgcggta aaacgcatcg taaataaaga acctcacaag ggtgtcaatc ctgatgagag
     4321 tatagctgct ggtgctgcta tccaagctgg tgttcttgca ggtgatgtag gtgatatttt
     4381 actccttgat gttactcctc tttcccttgg tattgagaca ctcggaaatg tatttaccaa
     4441 actgattgaa agaaacacta caataccaac aaaaaagagt caaatattta gtacagcaac
     4501 agacaatcaa cctgcagtag atattcatgt acttcaaggg gaaagaccaa tgtctacaga
     4561 caacgtgacc ctaggacgtt tccaactcga tgggattcca ccagcacgcc gtggcattcc
     4621 acaaattgaa gtaacctttg atattgactc caatggcatt gttcatgtct ctgcaaagga
     4681 cctgggtaca ggaaaagagc aatctattaa tataactgcc agcgctaata tgtctgaaga
     4741 agaaattgaa aagaaagtta gggaagcaga agaatttgct caagaagata agaagcgtca
     4801 agagttggca gaagccaaaa ataatggtga tgctctagct tatcaagctg aaaaaacaat
     4861 tgatgaacta ggtgatcaaa ttgaagagga agaaaagaca aaaatcgaag gattgatttc
     4921 ttcattgaaa actgctcttg aaaatgatac actagatgag
//
LOCUS       DASRNA010000039         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_680927,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000039 DASRNA010000000
VERSION     DASRNA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680927"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1457
                     /locus_tag="VFD08_01160"
     CDS             117..1457
                     /locus_tag="VFD08_01160"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZK09653.1"
                     /translation="MKFLFNSKKDVELDLSFNEEGLKHGELFVSLDKDRKGFVSMNLK
                     KRDDESLRRAFFTLASKLNELKVEALSMDLGDLSPKAVYEGFLQSTYFFDHYLSEKKT
                     RYFKEVSFFLDESLMDEVKNDFMELEQLLDGVFLARDLTNLSAIDLYPSTLAQKAQEE
                     LTPLGVDVQIMGQDEIEKLGMEAFLAVSYGSDKEPKFIVMTYKNSDEQPIALVGKGLT
                     YDSGGYSIKPADGMLTMHGDMAGAAAVIGAMKAIASNKLSSHVVAIVAATENLIDGSA
                     FKTGDIIGSMSGKSIEVVNTDAEGRLTLADALYYATTVVKPRYVIDTATLTGAMAVAL
                     GNLSAGAVYNDKALVDKVSAAGEKVGEPVWALPNFEEYKEKNKSHFADIKNSGGRMGG
                     GISAGMFLGEFVVDTPWVHIDIAGVSYEDSQRAYYPKGGTGYLVKTLYQLVKDE"
     gene            1516..1989
                     /locus_tag="VFD08_01165"
     CDS             1516..1989
                     /locus_tag="VFD08_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09654.1"
                     /translation="MCVLLYKPFVLGYLAVAIILQVLIYFKRIILIPRGLDGGVMVMF
                     LAAFFMSQRLVLRGSLGLRLIILLGLGIFFYGGSKLIFPGQYKSLLKLKEPEEFLDLL
                     EEGEEFEEKKEVFKLKKGTSSKKRINEHLRKTSKPIFELLMALFLGGVFVYFLIN"
     gene            2078..3358
                     /locus_tag="VFD08_01170"
     CDS             2078..3358
                     /locus_tag="VFD08_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZK09655.1"
                     /translation="MDEVDWWLFALLLFFIALGGFFAGAEISLASVNQSRIKIRSDKG
                     EKRASKTLYLLRHFDQTLSTILIGNNISHVFSASLAALLVTKIWGTGALVYSTISITI
                     IVFLISEMLPKTLAKAYSETASLWVSPLLYYLTIIFRPISALLTGLGNIISKLFDDES
                     RATVTEEEFYNIIETIKNENALEDQKGQWIHTALNFGDKSVENVLTVRTDIVAVDIMD
                     DPAEVYETIKETKHSRIPIYKDNIDNIIGILLTRRYLRASYQLGKAADIMEYIDPPLF
                     VFSSTDIDELLPEMSRKKVNLAIVTNTFGGVVGLVTVEDILEELVGEIWDEDDIAIEY
                     FYKLRKSCYEVDASVSVGEVFEKLNLDDYELDDLAHESMGSWVYESFGRIPSIGDKIE
                     SQGLCIEVSQMDKRRIMKLIVSLPSEKKEEALCK"
     gene            3349..>4560
                     /locus_tag="VFD08_01175"
     CDS             3349..>4560
                     /locus_tag="VFD08_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZK09656.1"
                     /translation="MQVILSIIGLLLMSAFFSSSEIAYSSINRVRLESMQDGKNKRAT
                     MAYYIYNHYEKALATILIGNNLVNIAASTLATYLAITLVGERAVTGAAIIMTLLILIF
                     GEITPKIIASKHSLTTALFASYPLRFFMRIFYPIIWVTGPIFSILKRRFKGEELDPEE
                     QSEAVEEELVTLLDTVEYEGVINENRRNIISSALDFTERSVSEILIPRVDLYAIDLDD
                     ERQDILEQIQGSPYTRVPMYDKTIDNIVGILHMNQFYRNIIKDPDLDLRKNLRPAIYL
                     YKTTKLPVALEKLRQSQMHLGIVTDDYGGNIGIVTIEDIMETLVGEIWDETDEIHDDV
                     IRLSDKSYELDGDLSISDFIDFMGWDEDKFDFESDTVGGWCMEMAQGYPRAGDNFEYD
                     NVQITVLEMDHL"
BASE COUNT         1351 a          762 c         1033 g         1414 t
ORIGIN      
        1 aaaaatacct atatactatg aattatacac ttaaataagg ttttttgtgg ttaattctta
       61 gggtaagggt atataagcta gagtgagctt actgtaaaag aaaaacagga ggaagaatga
      121 aatttttatt taatagtaaa aaggatgttg aactagatct tagttttaat gaagaaggct
      181 taaagcatgg cgagcttttt gtttctttag ataaagatcg caagggtttt gtctctatga
      241 acttaaagaa gcgtgatgat gaatctcttc gtcgggcttt ttttaccctg gctagcaagc
      301 taaatgaact caaggttgaa gcccttagca tggatttagg ggacttgtct cctaaggcag
      361 tatacgaagg ttttttgcaa agtacgtatt tttttgacca ttatcttagt gagaagaaga
      421 ctcgttattt caaagaagtg agcttctttc ttgatgagag cctgatggat gaagttaaaa
      481 atgactttat ggagctggag cagctcttag atggtgtgtt tttggcaaga gatctgacca
      541 atctttctgc catagatctc tatccttcga ccctagcgca aaaagcgcaa gaagaactca
      601 ctcccttggg tgttgatgtt caaattatgg ggcaagatga aattgaaaaa ttaggtatgg
      661 aagcctttct ggccgtaagc tatggttctg ataaagagcc caaatttatt gttatgacct
      721 ataaaaatag tgatgagcag cccattgcct tagttggcaa gggtctgacc tatgactctg
      781 gtggttatag tattaagcca gctgatggca tgcttaccat gcatggtgat atggcaggag
      841 ccgcggctgt aattggggct atgaaggcga tagcatccaa caaactttct agccacgtag
      901 ttgccatagt agctgcaaca gaaaacctta ttgatggaag tgcttttaaa acaggcgata
      961 ttataggctc tatgtcaggt aagagtattg aagtggtaaa taccgatgca gaggggcgcc
     1021 ttacccttgc tgacgcctta tactatgcaa ccacagtggt aaaaccacgc tacgttattg
     1081 atacagcgac ccttacaggc gccatggctg ttgccctggg aaatcttagt gcgggagctg
     1141 tctataatga caaggcccta gtagacaagg tgtctgctgc aggagaaaaa gtgggagagc
     1201 cagtttgggc cctaccaaat tttgaagaat ataaagaaaa gaataaatcc cattttgctg
     1261 atataaaaaa tagtggcgga cgtatgggag ggggcattag tgctggtatg ttccttggcg
     1321 agtttgtagt agatactccc tgggttcata tagatattgc aggggttagc tatgaagata
     1381 gccaaagggc ctattaccct aaggggggaa caggctacct tgttaagacc ctatatcaac
     1441 tggtaaagga tgaataaact ctcttaaagg ccttcctccg gggaggcctt tttaccctat
     1501 ctttaggagc tagctatgtg tgttttacta tataagccct ttgtactagg ctatcttgcg
     1561 gtggctatta ttttgcaagt tctaatctat tttaaaagaa tcattcttat tccgcgggga
     1621 cttgatggcg gagttatggt gatgttttta gcggcctttt ttatgagtca aagactagtt
     1681 cttagaggat ctttagggct gaggctgata attcttttgg gcctgggaat tttcttttat
     1741 ggtggaagta agcttatttt tccgggccag tataaaagtc ttttaaagct taaagagccg
     1801 gaggagtttt tggaccttct tgaagagggg gaggaatttg aagaaaaaaa agaggttttc
     1861 aagctaaaaa aaggaacttc ttcaaaaaaa cggatcaatg aacatttaag gaagacttct
     1921 aagccgatat ttgaactcct tatggccctt tttttaggtg gagttttcgt gtattttcta
     1981 atcaattaag ccccttgtag taaattgaca ataaggggaa tttcatatac actttaacta
     2041 cttgaaagat acgttttaga aggaggaaaa ataccttatg gatgaggttg attggtggct
     2101 ttttgcttta cttctttttt ttatagccct tggtggtttc tttgcaggag ctgagatttc
     2161 cttagcttct gttaatcaat cccgaattaa aattcgctcc gacaaggggg agaagagagc
     2221 ttcgaaaacc ctctacttac tccgacattt tgatcaaact cttagtacaa ttttaatagg
     2281 gaataatatt tctcatgtat tttcggcttc ccttgccgcg ctcttagtga ctaaaatttg
     2341 gggaaccgga gccctggtct attctaccat tagcattaca attatagtgt ttttaatcag
     2401 tgagatgtta ccaaagacgc tggccaaggc ttatagcgaa acggcctccc tttgggtatc
     2461 ccctctactg tattatctga ctattatttt tcgccctatt tccgctcttc ttacaggcct
     2521 tggaaatatt atttcaaaac tctttgatga tgaaagtcgg gccactgtta cagaagagga
     2581 attttacaat atcattgaaa caattaaaaa tgaaaatgcc ttggaagacc aaaagggtca
     2641 gtggatacat actgccctta attttggaga taaaagcgtc gaaaatgtcc ttactgtacg
     2701 cacagatatt gttgctgttg atattatgga cgacccggca gaggtttatg aaaccataaa
     2761 agaaaccaaa cattctagaa taccaatata caaagataat attgacaata taattggaat
     2821 ccttttaact agacgctatt tacgggccag ttatcagctg ggcaaagccg ctgatattat
     2881 ggaatatatt gaccccccgc tttttgtttt tagcagtaca gatattgatg aattattgcc
     2941 agagatgagc agaaaaaaag taaacctagc catagtgaca aatacttttg gtggggttgt
     3001 aggcctggtg acggtggaag atattttgga agaactcgtc ggtgaaattt gggatgagga
     3061 tgatattgcc attgaatact tttacaaatt aagaaaaagc tgttatgagg tcgatgcttc
     3121 agtaagtgta ggggaggttt ttgagaagct taatctggac gactatgaac tggatgattt
     3181 agctcatgaa agcatgggaa gttgggttta tgaaagtttt ggtcgaattc cctctatcgg
     3241 tgataaaata gaaagccagg ggctctgcat tgaggtttct caaatggata agagaaggat
     3301 tatgaagctt attgtttcct tgccttcaga aaaaaaggag gaggccttat gcaagtaatc
     3361 ctaagtatta ttggcctact tttaatgtca gctttttttt cctcctctga aattgcctat
     3421 tcctctatta atagagtgcg acttgaatct atgcaggatg gaaaaaacaa aagagcgacc
     3481 atggcttatt atatatacaa tcactatgaa aaagccctgg ctacgattct tattggcaac
     3541 aatttagtca atatagccgc ctctactctt gcaacctatc ttgctattac tttggtagga
     3601 gaaagggctg taacgggagc tgctattatt atgactcttt tgatcctaat ttttggagaa
     3661 attacgccga aaattattgc atcaaaacat tctttaacaa cggcgctttt tgcctcctat
     3721 cccctacgct tttttatgag aattttttat cctattattt gggttactgg gccaattttt
     3781 agcattttaa aaaggcgctt taaaggagaa gagcttgatc ccgaagaaca gtccgaggca
     3841 gtagaagaag agcttgtgac ccttcttgat acggtggagt atgagggggt aatcaatgaa
     3901 aacagaagaa atattattag ctctgcctta gactttaccg agcgctcggt aagtgaaatt
     3961 ttaattccta gagtggactt gtatgccatt gaccttgatg atgagcgcca ggatatttta
     4021 gagcaaattc aaggctcacc ttatacaagg gtacccatgt atgataagac catagacaat
     4081 atagtgggga ttcttcatat gaaccagttt tatagaaata ttattaagga ccctgatctt
     4141 gatttgcgaa aaaatcttag gccagctatt tatctctata agacaacaaa gcttcctgtt
     4201 gccctagaaa aactccgtca gtctcagatg caccttggca ttgtaacgga tgattatggt
     4261 ggtaatatcg gcattgttac cattgaagat attatggaaa ccctggtagg ggaaatttgg
     4321 gatgagacag atgaaatcca tgatgatgtt attcgactct ctgataaaag ttatgaactt
     4381 gatggagatt taagcataag cgactttatt gattttatgg gctgggatga agataaattt
     4441 gattttgagt cagataccgt aggtggttgg tgtatggaga tggcccaagg ttatcccagg
     4501 gcaggagaca atttcgagta tgataatgtt caaattaccg ttttagaaat ggaccatttg
//
LOCUS       DASRNA010000040         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_532331,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000040 DASRNA010000000
VERSION     DASRNA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532331"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFD08_01180"
     CDS             complement(<1..868)
                     /locus_tag="VFD08_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK09657.1"
                     /translation="MKLFRSELKELSSYVPGKPLEEVMEDYGLSRIIKLASNENPLGP
                     SPLAIEAVKNEAENMHLYPDPEARLLKRALAEELGVSTEQILFGNGGEATIQLVAASV
                     IEKGDEVVVPEPGFALHEISTAHMGGIVKKVPLLPDYSHDLEGMLGVVNEKTKIVFLT
                     NPNNPTGVMIKKEELEDFAARLPEQVLLFIDEAYFEFAKIDPHYPDGLEILKKRKNTL
                     VLRTFAKVCGIAGIRLGYIVSTPEIIKEVSKSAGVFSVNRLAQVAGLASLKDKEHIEK
                     SVALARASLARMM"
     gene            complement(881..1651)
                     /locus_tag="VFD08_01185"
     CDS             complement(881..1651)
                     /locus_tag="VFD08_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK09658.1"
                     /translation="MLLEVKNIHKHYPYLSRRLFPGKKKFVALENINFQLERGKTLGI
                     VGESGSGKSTLGGIVGDLRKPTLGEVLYKGKNIEKMTKEEYRDYRRNVQFIFQSPQES
                     MNPGYSIKKVLHEPMDILLNLTQKEKEDRIDTTLSIVGLEKEILSKSPREISGGQAQR
                     VAIARSIMLRPEIIICDEPTSALDVSIQAQALNLLRRLQEELGISYLFITHNMALINY
                     MCDELMVLYKGKVVEQGPTEEVIQNPKEDYTKALMSFV"
     gene            complement(1642..2481)
                     /locus_tag="VFD08_01190"
     CDS             complement(1642..2481)
                     /locus_tag="VFD08_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK09659.1"
                     /translation="MKGIRVKNLCIYDETNEQVLLDHISFELEEGKSLGIVGESGGGK
                     TLTVLSLMNLIQPGLRREADVLEVLGEDLLTQRRKDRHRFLGTKVGYVPQNTFAYLHP
                     YYKVKNQIADGYIYHFNKSKKEALERAQSLLGQVGFEEPKRVLEAFPGELSGGMRQRV
                     NIAMALMNDPRLLVTDEPTAALDVMVAKQVMDLFSTVHQERKNTFILVSHDLNLVRNY
                     CDEILILKKGKVVEMGATEQIFTAPKKDYTKALIAVVPTLSYPKDQPLKELKDFIEGE
                     ICS"
     gene            complement(2468..>2765)
                     /locus_tag="VFD08_01195"
     CDS             complement(2468..>2765)
                     /locus_tag="VFD08_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK09660.1"
                     /translation="NIVLQHMLPQMMPKLLTQFTSSMSRAILTEGSLSFLGLGIQPPA
                     ASLGLMLSEARQHYLVHPYLGIPAGLVLMMAVLGFSLLGDYVNDLWIGGQSEGD"
BASE COUNT          673 a          693 c          592 g          807 t
ORIGIN      
        1 ccatcattct agcaagagaa gctctagcca gggccacact tttttctata tgttccttgt
       61 ctttaaggga ggccagtcct gccacctggg ccaggcggtt gacactaaaa acccccgcgg
      121 acttagaaac ttctttaata atctctggag tggacacaat atacccaagg cgaatgcccg
      181 caattccgca gactttagca aaggtgcgca ggaccagggt gttttttctc ttctttagga
      241 tctccaggcc atcgggataa tggggatcta ttttggcaaa ctcaaaataa gcttcatcga
      301 taaaaagcag gacctgctct ggcagcctgg ctgcaaagtc ttctagttct tcttttttta
      361 tcataactcc cgtgggattg ttgggattgg ttaaaaaaac aatttttgtt ttctcattta
      421 ccacccccag catgccctcc aggtcatgac tataatcagg tagcagggga acttttttca
      481 caataccgcc catatgggca gtggaaatct catgcagggc aaagcccggt tcgggcacca
      541 ctacctcatc gcctttttct atgacactgg ctgccactag ctggatggta gcttccccgc
      601 cattgccaaa aagaatctgc tctgtactca ctcctagctc ttctgccagg gctcttttaa
      661 gcagcctggc ttcggggtca ggataaagat gcatattttc tgcttcattt tttaccgcct
      721 ctatggccag gggagaaggc cctagggggt tttcattgct ggcaagcttg ataatcctgc
      781 tcaggccata gtcttccatt acttcttcta ggggcttgcc aggcacatag ctggaaagct
      841 cctttagttc acttctaaat aatttcattt tgcctcctag ttaaacaaag ctcataaggg
      901 cctttgtata gtcttccttg ggattttgta tcacttcttc tgtaggacct tgttccacta
      961 cttttccctt atataatacc ataagctcat cgcacatata attgattagg gccatattgt
     1021 gggtgataaa aagataacta attcccagtt cttcttgtag gcgccttagt aaatttaggg
     1081 cctgggcctg gatactcaca tcaagggccg atgtgggctc atcgcaaata ataatctcag
     1141 gtctaagcat aatgcttcta gcaatggcca ccctctgggc ctggccccca gaaatctccc
     1201 tggggctttt acttaaaatt tctttttcaa gaccaacaat actaagagta gtatcaatgc
     1261 ggtcttcttt ttccttctgg gtcaagttaa gcaggatatc catgggttca tggagcacct
     1321 ttttaatact atagccagga ttcatggatt cctggggact ctgaaagata aactggacat
     1381 tgcggcggta gtcccggtac tcttcctttg tcatcttttc tatgtttttt cccttataga
     1441 gaacctctcc cagggttggt tttctcagat ctcccacaat cccccctagg gtggactttc
     1501 cactaccaga ctctcctaca atacccaggg tctttcccct ttctagttga aaattgatat
     1561 tttctagggc aacaaacttc ttcttgcctg gaaaaagcct gcgacttaga tagggatagt
     1621 gcttatgaat gttttttact tctaagagca tatttccccc tcaataaagt cctttagttc
     1681 cttaaggggc tggtccttgg gatagcttag ggtaggaacc accgcaataa gggccttggt
     1741 atagtctttc ttgggcgcag taaagatctg ctcggtagct cccatctcca ctaccttgcc
     1801 cttttttagg ataagaatct catcacaata attgcgtacc agattcagat catgactgac
     1861 caggataaag gtgtttttcc tctcctggtg gaccgtggaa aaaaggtcca taacctgctt
     1921 ggctaccatt acatctaggg ccgctgttgg ttcatcggtt acaagcaggc ggggatcatt
     1981 cattagggcc atggcaatat ttaccctttg gcgcatgccc ccgctaagtt cacccggaaa
     2041 ggcctcaaga accctttttg gctcttcaaa gcccacctgg cctaaaagac tctgggccct
     2101 ttccagggcc tcttttttac tcttattaaa gtgatagata tagccatcag caatttgatt
     2161 ttttaccttg taatagggat gcagataggc aaaggtattt tgtggcacat agcccacctt
     2221 ggttcctagg aacctatggc ggtcctttct tctctgggtt agaaggtctt ctcctagaac
     2281 ctctaagaca tcggcctctc ttcttaggcc tggctggatt agattcataa gagaaagaac
     2341 cgtcagggtc ttgcccccgc cagactctcc tacaataccc agactctttc cctcctcaag
     2401 ctcaaagcta atatgatcaa gcaggacttg ttcattagtc tcgtcataaa tacaaaggtt
     2461 ttttacccta atccccttca ctttggcctc caatccacag atcattaaca taatccccta
     2521 gcagggaaaa gccaagaact gccatcataa gcacaagccc tgctggaatg cccagatagg
     2581 ggtggaccag gtagtgctgg cgggcctcgc taagcataag tcctagactg gccgcaggag
     2641 gctggattcc cagccctaga aagcttaggc ttccttcggt caggatggcc cgggacatgc
     2701 tagaagtaaa ctgggtaagg agcttgggca tcatttgagg cagcatatgc tggaggacaa
     2761 tgtta
//
LOCUS       DASRNA010000041         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_610497,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000041 DASRNA010000000
VERSION     DASRNA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610497"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFD08_01200"
     CDS             <1..709
                     /locus_tag="VFD08_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZK09661.1"
                     /translation="LSAVGEGNPQLLWDINVNGLYNALEVSRELDLALFVPSSIGAFG
                     PSTPKTSTPQTTIQRPTTAYGVSKVAGELLCDYYHKRYGLDTRGVRFPGLISYKALPG
                     GGTTDYAVHIYYDALEKGNYTSFIGKGTYMDMMYMPDAVECIIDLMEADPHKLIDRNA
                     FNVSGMSFEPEEMKKSIQKHLPNFVLDYDVDPVRQAIADSWPDSLDVTAAKEQWGFDP
                     KYDLDAMTKDMLEKLAK"
     gene            772..1668
                     /gene="ftcD"
                     /locus_tag="VFD08_01205"
     CDS             772..1668
                     /gene="ftcD"
                     /locus_tag="VFD08_01205"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002672932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HZK09662.1"
                     /translation="MKGPLIECIPNFSEGQKPQVIEAILDTLRAKDGIRLLDYSSDKD
                     HNRTVVTLVGTPEGLLTHIPLFVKKAKELIDLRQHKGQHPRMGAVDVLPFVPVRGITM
                     EETVDLSKKMAQKLHEQLDVPIFLYESSASAKHRENLASLRKGQFEGMAEKIKKEEFK
                     PDYGRELHESFGIMAVGARAFLVAFNVNLATDKLEIANQIAKNVRHISGGLRFVKGMG
                     VALEDRGQVQVSMNLTDFSKTPIHRVFELIKIEASRYGVSVVGSEIVGLVPMAAMIDT
                     AAWYLKTENFSEEQVLEAKLME"
     gene            1849..2466
                     /locus_tag="VFD08_01210"
     CDS             1849..2466
                     /locus_tag="VFD08_01210"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HZK09663.1"
                     /translation="MLLDKTVKEFIDEVASDKPVPGGGSVSALVGGLSAALLEMVTSL
                     TIGRKKYLHVEEEMLKLRAEFVGLRKSLLHLMQEDVVSYSRVINAYKLPKDTIEEKAK
                     RTEAIQEGLYGAALVPLEVAKVCLESLQNVEEVLTKGNKNAYSDAKVAGVMLRSALYG
                     ACYNVQINLEGLKDKERIEFLSKSIDYLRNEADLLERRIIDLEVS"
     gene            2466..>2864
                     /locus_tag="VFD08_01215"
     CDS             2466..>2864
                     /locus_tag="VFD08_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK09664.1"
                     /translation="MRIVSDSSLDIRPPVEGVDLAVIPFIIRFDNNEWEDMDWSQLPE
                     YEKDMVKSKSFKTACPSPNQWLEAFKNADGPIFGITISSRLSGSYNAALLGKELYSQM
                     GGKAKIHIFDSKSAGGGPTLIYYKIQELYKE"
BASE COUNT          905 a          471 c          668 g          820 t
ORIGIN      
        1 tctttccgct gtgggcgagg gtaatcctca acttctttgg gacattaatg taaatggtct
       61 ttataatgcc ttagaagttt ctagggaact tgatttggct ctttttgttc caagttctat
      121 tggtgccttt ggaccaagta cacccaaaac aagcacaccc caaacaacga ttcagcgtcc
      181 tacaacagcc tatggtgtta gtaaggtagc tggcgaactc ctctgtgatt attatcataa
      241 gcgttatggt cttgatactc gtggagtgcg ttttccaggg cttatttcct ataaggccct
      301 accagggggc ggaacaactg actatgcggt acatatttac tatgatgctc ttgaaaaagg
      361 aaactatact tcatttattg gaaaaggcac ctatatggat atgatgtata tgcctgacgc
      421 tgttgagtgt attatcgatc ttatggaagc agatccccat aagttgattg atcgaaatgc
      481 ttttaatgta tcaggaatga gcttcgaacc agaagaaatg aaaaaaagta ttcaaaaaca
      541 tctgcctaac tttgtccttg attatgatgt tgatcctgtg cgccaagcaa ttgcagactc
      601 atggccagat tctttagatg taactgctgc aaaagagcag tggggctttg acccaaaata
      661 tgatttagat gccatgacaa aagatatgtt agaaaaactt gctaagtaag gagaagtctt
      721 tgtctgctag ccttggcatt aaaaggatag aaatccttgt aaggagaaaa aatgaaagga
      781 ccattaatag aatgtattcc aaactttagc gaagggcaaa aaccccaagt aattgaggct
      841 atattagata ctcttcgagc aaaggatggc attcgtcttt tagattatag ttcggataaa
      901 gaccacaatc gtactgtagt aacccttgtg gggacaccag aggggctttt aactcatata
      961 cctttatttg ttaaaaaagc caaggaactg attgatttac gccagcacaa gggccaacat
     1021 cctcgaatgg gagcggtaga tgtgcttcct tttgtaccgg tacgtggtat taccatggaa
     1081 gagactgttg atttaagtaa aaaaatggca caaaagctcc atgaacaatt agatgtgcct
     1141 atatttttat atgaatcatc ggcttctgct aagcatcgtg aaaacctggc cagcctaaga
     1201 aaaggtcaat ttgaaggtat ggctgaaaaa atcaaaaagg aagaattcaa acccgattat
     1261 ggtagggagc ttcacgaaag ttttggaatt atggctgtgg gagcaagagc gtttttggtt
     1321 gcttttaatg tgaatctagc aaccgataag ctagaaatag ccaatcaaat tgctaaaaac
     1381 gttcgccata taagtggagg attacgcttt gttaagggca tgggtgttgc tttagaagat
     1441 agaggacagg ttcaagtttc aatgaatctt acagacttta gcaaaacacc tatccataga
     1501 gttttcgaac taataaaaat cgaagctagt cgatatggtg tcagtgttgt tggcagcgag
     1561 attgtaggcc tggtccctat ggcggccatg attgatacag cggcctggta tttaaaaaca
     1621 gaaaacttta gtgaagagca agttcttgaa gcaaagctta tggaataaag aaattcccat
     1681 aggtttttct agatagaaaa aacgaataag ggttaggcta aggccaaacc cttttttaca
     1741 tagactataa gaggatagat taaagctagt aaaggccctt tatgtgatga gggcagttta
     1801 ttgggtagta aataaaagta agcttgtttt aatattaggg ggaaatttat gttgctggat
     1861 aaaacagtaa aagaatttat tgatgaggtg gcttctgata agccagtacc tggtggggga
     1921 agcgttagtg ccttagttgg aggtctctct gctgcccttc ttgagatggt gacaagtcta
     1981 accattggtc gaaaaaagta tcttcatgtg gaagaagaaa tgctaaagct tcgggcagaa
     2041 tttgtagggc tgcgcaagag ccttcttcac ttaatgcagg aggacgtcgt cagttatagt
     2101 agggtgataa atgcctataa acttcctaag gatactatag aagaaaaggc caaacgaaca
     2161 gaggcaatac aagaaggact ttatggggca gcccttgtcc cccttgaagt ggcaaaagtt
     2221 tgcctagaaa gccttcaaaa tgtagaggaa gttcttacaa agggtaataa gaatgcctat
     2281 agtgatgcaa aggttgccgg tgtaatgctt cgaagcgccc tttatggtgc ttgttataat
     2341 gttcaaatca atctagaggg cttaaaagac aaagaaagga ttgagttttt atcaaaaagc
     2401 attgattact tacgcaatga ggcagatctt ctcgaaagaa gaattattga tttggaggta
     2461 agttaatgag aattgtttcc gatagtagtc ttgatattcg tcccccggtt gagggggtgg
     2521 atctagctgt gattcccttt attatacgct ttgataataa tgagtgggag gatatggatt
     2581 ggtctcagct gccagaatat gaaaaagata tggtaaaaag taaaagcttt aaaactgctt
     2641 gtccttctcc taatcagtgg cttgaggcct ttaagaatgc ggatgggcca atctttggaa
     2701 taactatttc ctcacgctta agtggcagtt ataatgctgc tttattagga aaagagcttt
     2761 attctcaaat gggtgggaaa gcaaaaatcc atatttttga tagtaagagt gctgggggag
     2821 gacccacact catctattat aaaattcaag aactgtataa agag
//
LOCUS       DASRNA010000042         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1057660,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000042 DASRNA010000000
VERSION     DASRNA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057660"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFD08_01220"
     CDS             <1..627
                     /locus_tag="VFD08_01220"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HZK09665.1"
                     /translation="VLLMSGGIDSPVAAYQMARRGVRVLPLHFHAQPFTSLESLEKVR
                     SLVKRLSYYMGPMSFYHMNLLETQQILKESCNDKYFTILQRRLMTKLAAKLASREKAL
                     GLITGENLGQVASQTIEGINCTNDASNIPVFRPLISFDKEDIVKIARDIETYETSILP
                     FEDACTVFLPSRVETRPRLRAIHKEEQKVDGNELINIAWKSLVKETIK"
     gene            complement(710..>3023)
                     /locus_tag="VFD08_01225"
     CDS             complement(710..>3023)
                     /locus_tag="VFD08_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZK09666.1"
                     /translation="YIYNPKVEERYSFVLQLMYEQGFISLEELEDGLAIDLKTKLNPG
                     KFYNDNIDNFFVETCQNQVIEDLVEQKGISVEQANNLLLTSGFTIHSTLIEEEQQFLQ
                     RQYMEEGSEEFFNAALTSSLSQFQAYHDLYDDGIMGEDTWQLLIDRGYFPTSINRLQL
                     SQGDEHRYVADIKRAVASEGFAPIHPWISYLSPTFSYGRIVLSDGYLYDYKSSFDQSS
                     NFLLPIDYFYQTDEGDIRVPDNGLLMFQETEGGYIYLGLGDMYHFGNNPQTREYKYDG
                     KYTLPYFLLFENGSLTIPNDYLAISNDELVISRSAFDDDVIFINDFGELAVSNEYVLK
                     STMPVVQPQSASAIIDESTGHLKAIFGGRGVLGHQLLNRALTPAQTGSAIKPLAVYGP
                     GIDSGILTAAETALDKPSYQFNTASNEPWPVNFSGTYEGPMTVRRALTISQNVPAVTF
                     ASMIGYELIADYLEANGITTIVREGAYNDLGPSALALGGLTLGISPVEMASAYGTFGN
                     EGVHMPWISYTHVEDDEGNIILDNRNHQGNRVFSPEANYIMTDLLNDVVTKTSFEAII
                     GGGIPVIGKTGTTGARKANTDAWFVGVTPYLSCSIWVGTDRNVSLTEGSWYVAQYWGK
                     MMKDYHANLPSAYFAPKPDGVGWLTVDARSGKLPGPYSKNTITELFILGTAPTEVDDS
                     YEAHIVCKVSGKAPTEYCEETEEEIFLKKEYAVTEADKAQVFDPDDQCEICEKEAKEK
                     KAEEEAEREEAEREEAEKEEAETEGNENGG"
BASE COUNT          886 a          627 c          602 g          908 t
ORIGIN      
        1 gtgcttctta tgagtggggg gattgattct cctgtggctg cctatcaaat ggctagaaga
       61 ggggtgcggg ttttgcccct gcattttcat gcacaaccct ttacaagtct tgaatcctta
      121 gaaaaggtac gtagtctagt aaaaagactt agttactata tgggccccat gagcttttat
      181 catatgaatc tgcttgagac ccaacagatt cttaaagaaa gctgcaatga taagtatttt
      241 accattcttc agagaagact tatgacaaag cttgcagcta aactagcaag tagggaaaaa
      301 gcccttggct taataacggg ggaaaatcta ggccaggttg catcacaaac aattgaggga
      361 atcaattgta ccaatgatgc cagcaatatc cccgtattta ggcctcttat tagttttgat
      421 aaagaggata tagtaaagat agctagggat attgaaacct atgaaacatc tattttaccc
      481 tttgaagatg cttgcacagt ttttttgccc tctagagtag aaacccgtcc caggttaagg
      541 gcaattcaca aagaagaaca gaaagttgat ggaaacgaat taataaatat tgcatggaaa
      601 agtttggtta aagaaacgat aaaataaaac tggccaaagg gccagttttt agtttgcagt
      661 taaattggat ttaaggaaga agaggccact ggggaagcca ctcagatagt catccgccat
      721 tttcgtttcc ttctgtttca gcttcttctt tttcggcttc ttctctttca gcttcttctc
      781 tttcagcttc ttcctctgcc tttttttcct tggcttcttt ttcgcaaatt tcacactgat
      841 catctggatc aaaaacctgg gccttatcag cctctgtaac agcatattct ttttttagaa
      901 aaatttcttc ttctgtttct tcacaatatt ctgtaggtgc ttttccagat actttacata
      961 ctatatgagc ttcataagaa tcatcaacct ccgttggtgc ggtaccaagg atgaataatt
     1021 cagttatggt gtttttagaa tagggccccg gaagtttacc agatcttgca tctactgtca
     1081 gccatccaac cccatcaggc tttggtgcga aataagcact aggcaaattg gcatgatagt
     1141 ccttcatcat tttcccccaa tattgggcta cataccagga accttctgtt aaggaaacat
     1201 tcctatctgt gcctacccag atactacagg aaagataggg agtaacacca acaaaccatg
     1261 cgtcagtatt tgcttttcta gcccctgtag ttcctgtttt tccaataacg ggaatacccc
     1321 caccaataat ggcttcaaag gaggtttttg ttacaacatc attgagcagg tcagtcataa
     1381 tgtaattagc ctctgggcta aaaactcggt tgccttgatg gtttctatta tctaagatga
     1441 tatttccctc atcatcctcc acatgggtat aggaaatcca gggcatatgc acgccctcat
     1501 ttccaaaggt tccataggcc gaggccatct caactggaga aatacccagg gtcaggccac
     1561 ctagagctag ggcactagga cccaggtcat tataggcgcc ttctcttaca atagtagtga
     1621 ttccatttgc ttctaggtaa tcggcgatta gctcgtaacc tatcatactg gcaaaagtaa
     1681 cagcaggaac attttgagaa atggtaagtg ccctacggac tgtcatagga ccctcataag
     1741 ttccactaaa gtttactggc caaggttcat tcgaagcagt attaaactga taactaggtt
     1801 tatctagggc cgtttcagct gctgttagaa tgccagaatc aattcctgga ccataaacag
     1861 caagaggctt aatggcagaa ccagtttggg ctggcgtgag ggctcggttt agtaactgat
     1921 gtcctaaaac acctcgtccg ccaaagatgg cctttaggtg tcctgtactt tcatcaataa
     1981 tagccgaagc actttgaggt tggacaaccg gcatagtact ttttagtaca tattcatttg
     2041 aaacagcaag ctctccaaag tcgtttataa aaatcacatc atcatcaaag gccgatcggc
     2101 tgataacaag ttcatcattg gaaatggcta gataatcatt gggaatggta agggaaccat
     2161 tttcaaaaag aagaaaatag ggtagtgtgt acttaccatc atacttgtat tctcgggttt
     2221 ggggattgtt tccaaagtgg tacatatctc ctagtcctag ataaatatat cctccctctg
     2281 tttcttggaa catcagaagg ccattatcag gaacccgaat atctccctca tctgtttgat
     2341 agaaataatc tataggaaga aggaagttgc tactctgatc aaaactggac ttataatcat
     2401 aaaggtatcc atcgctcaat actatacggc cataactaaa ggttggactc agataactaa
     2461 tccaggggtg aatgggagca aagccttcgc tagcgacagc tcttttaata tcagcaacat
     2521 agcggtgttc atccccttgt gatagctgca gcctatttat gcttgtaggg aaataacccc
     2581 tatcaataag aagttgccaa gtgtcttctc ccataatccc atcatcatag agatcgtgat
     2641 aggcttggaa ctgcgaaagg gaagaagtaa gggctgcatt aaagaattct tctgaaccct
     2701 cttccatata ctgacgctgt aaaaactgtt gttcctcttc gataagggtt gaatggatgg
     2761 taaaaccaga tgttagaaga agattgttgg cttgttcaac gcttatgcct ttttgttcca
     2821 caagatcttc tataacctga ttttggcaag tttctacaaa aaagttatca atattatcat
     2881 tgtaaaattt accaggattt aattttgttt taaggtcaat tgctagacca tcttcgagct
     2941 cctccagaga gataaatcct tgttcataca ttagttgcag aacaaagctg tatctttctt
     3001 ctacttttgg attgtaaata taa
//
LOCUS       DASRNA010000043         6608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_610879,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000043 DASRNA010000000
VERSION     DASRNA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610879"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFD08_01230"
     CDS             complement(<1..483)
                     /locus_tag="VFD08_01230"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZK09667.1"
                     /translation="MQKDKLTDKLFIYQNKEHFRYGIDAVLLAHFALELEGRVLDLGS
                     GTGILPLLLSGLDSLSEIVGLELQKEALELAKKSVQDNGLSNKITLVQGDLMEIENLF
                     SRESFDGLICNPPYFEKNHGLACKDMARTISRSEEAMTLDGLLRAVAYLLKPRAPIYM
                     I"
     gene            complement(509..1426)
                     /locus_tag="VFD08_01235"
     CDS             complement(509..1426)
                     /locus_tag="VFD08_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HZK09668.1"
                     /translation="MKIIGVRFKKGGKIYHFDPKDMDLKQEDYVIVETIRGLEYGQVV
                     LGPKDVPEDEIKYELKSVVRKAGPDDHLTHIENKEKEKRAFRICAEKIEKHGLEMRLL
                     NVEYTFDVSKIIFYFIADSRVDFRELVKDLASVFRTRIELRQIGVRDEAKFVGGLGSC
                     GRELCCSSWMGEFQPVSIKMAKDQNLSLNPTKISGVCGRLMCCLNYEEEHYRNMRKIL
                     PSKGTRLNYQKEDARVHEVNYLTGMIQLKIHETKEDVFDLIWVSYDDLFSEKDKSKKR
                     EEVKKVPKEKPKEVKKKKRPRRKKGRKKK"
     gene            complement(1423..2355)
                     /locus_tag="VFD08_01240"
     CDS             complement(1423..2355)
                     /locus_tag="VFD08_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09669.1"
                     /translation="MELIGFDQSFKQLSSLAKKRQLSHALIFTGIEGIGKKKLARKLA
                     AFLLDIPSQEQLDHYPDFLFIEEKTNIGVEEIKRVLQFVVEKPLYGKRKVLIINDAHK
                     MNAQAQNKLLKTLEEPPDFLTFLFVSSAPGRLLDTIRSRVVEYPLKPHSEEEIYRALE
                     GYEEKERRLAARFSGGSFQRAFAILEDEELRKLFFFPEKIFDALEEGNRLKLLDYARE
                     YGKERQMASLLIEHCMLWLRDLALVGQNRNTQNLYYQDRLDVLIRHSQSLGRNKIPLF
                     REELEKGQRRLAANCNPEILLWSCLLQLQEGMNQ"
     gene            complement(2355..2681)
                     /locus_tag="VFD08_01245"
     CDS             complement(2355..2681)
                     /locus_tag="VFD08_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HZK09670.1"
                     /translation="MKLLIIIMQEDDVNRVVNRLNKEKIGVTRLASSGGFLRRGNTTI
                     LIGAKDDQVELIKDVIREKCRSRVEMMPSIPVIAQGIITASEPIEVRVGGAIYFQVDV
                     EEFQKF"
     gene            complement(2678..3271)
                     /gene="lepB"
                     /locus_tag="VFD08_01250"
     CDS             complement(2678..3271)
                     /gene="lepB"
                     /locus_tag="VFD08_01250"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZK09671.1"
                     /translation="MKNNFKGLAGLGQALAEWLKAFAGSIAILALILLFIQPTSVVNT
                     SMLPNYREGDRLLVNSMSSPKRGDVVIFNSGLALSESRWQYLSRIQKIFLKPEDSMTL
                     IKRVVALPGERFRLEDGQIYIDEVPLEESYIKGTTQGNLDIVLPADNYLVLGDNRENS
                     LDSRDSTVGFVKKKDILGSVFFRYWPLNRIEYIGGSQ"
     gene            complement(3334..4641)
                     /locus_tag="VFD08_01255"
     CDS             complement(3334..4641)
                     /locus_tag="VFD08_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZK09672.1"
                     /translation="MKLREGLDRVIEEELVHFEMPGHKGRIEKQIYQYDFTEIPGTDN
                     LTNPQGIILQTIEEISKAFGSKGSFISVNGATGALLGAMSSAFSRGDEIIIMRNSHIC
                     IYDGVYLLGLKAKYLFSDGDLLAQLKELVSENTRGVILTSPSFYGEIIEDEVYEWLVS
                     KDLIIIIDEAHGSHLKLIDDRLSGMRYGDMVVHSFHKTLPSMTQTAILHLCTDRFKKS
                     YVQKNLKLFQSTSPSYVLMRSLDIAIDIYIYQGKELMQKLLLHCQDFKVQLEKTTDFR
                     LIYKEGKHDKTRLLLRHKLPIDYSHMEKELRKRGLQIEFQSDQGLLLIPSIMSTREDF
                     SRLLTALKQVQVRKKEKISYQVFRPKKAMEIREAFLKDSKVCSYQEALGEVVSEYIIP
                     YPPGSPILVPGEIMNKELVAYLESFKGNIVGLEKEGFVNILLD"
     gene            4772..5956
                     /locus_tag="VFD08_01260"
     CDS             4772..5956
                     /locus_tag="VFD08_01260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZK09673.1"
                     /translation="MLSKRASNISPSITLGISAKVKELKSKGQPIINLSIGEPDFFTP
                     DKVKEEGKLAITNNLTKYDAASGNLSLREAISEKLKKENFLSYQPEQIVVSSGAKHAI
                     TNALLATIDPGDEVLIPIPYWVSYPQMVSLVGGVPILVATKKEAGFKVSLDDIRPYLS
                     EKTKMIILTNPSNPTGNVFSKEELEVLCGELAKKDILILADEIYERVVYDEPFTSIPS
                     LSQEIYDKTILINGLSKSASMTGWRLGYSACNKTLAKAMGSIQSHLTSHPSTISQAAS
                     IVALRDCTEEVEHMVKTYKERRDYIQNFFKEWGVLEIIPPKGAFYVFIDISPLAAKLK
                     EESLSLRVCDDMIEEHKVAFIPGIGFGEDDFIRLSYAAALEEIKEGLERFKSYVEQIL
                     ED"
     gene            5946..6524
                     /locus_tag="VFD08_01265"
     CDS             5946..6524
                     /locus_tag="VFD08_01265"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZK09674.1"
                     /translation="MKIKSIEEKPLSYLKSFHITYEDKKGQPRIWEVASRGSKERLEK
                     EIQGEIFSDGAMIVAWDDKKEHVLMVREFRVISGHDVISFPAGLNDKDEDILTTAVRE
                     FKEETGLDLLPEGIDRPRYTSVGLSNERVYTVFGRFSGQISTAYQEDSERILPFLVDR
                     KKAIELLEKEDVTIRSAFILRGLFSLPLYEKL"
BASE COUNT         1798 a         1463 c         1286 g         2061 t
ORIGIN      
        1 gatcatatag ataggggccc tgggctttaa tagataggcc actgccctta aaaggccatc
       61 tagggtcatg gcttcttcac ttctagagat ggttctggcc atatccttgc atgctagtcc
      121 gtggtttttt tcaaaatagg gtggattgca aataagtcca tcaaagctct ccctggaaaa
      181 aaggttttct atctccatga ggtctccctg gacaagggta attttatttg aaagcccatt
      241 gtcttgcacg gattttttgg ccagctcaag ggcttctttt tgaagttcaa gaccaacaat
      301 ttcactaagg gaatctagcc ctgaaagaag aagggggaga atgcccgttc cactgccaag
      361 atcaagcacc ctgccctcta gttctagggc aaaatgcgca agaaggaccg catcaattcc
      421 atagcgaaag tgttccttat tttgatagat aaatagcttg tctgttagtt tgtctttttg
      481 catcttgata aaaaagtaga gctatactct actttttttt cctgcctttc tttcttctag
      541 ggcgtttttt ctttttcact tcctttggtt tttctttggg gaccttcttt acttcttctc
      601 tttttttact cttgtctttt tcactaaaaa gatcatcata ggaaacccag ataagatcaa
      661 aaacgtcttc tttggtttca tgaattttaa gctggatcat accagttaga taattgactt
      721 catggaccct ggcatcttct ttttggtagt tgagtctggt tcccttacta ggaagaatct
      781 ttcgcatatt tctataatgc tcttcttcat aatttaagca gcacataagt cttccgcaaa
      841 cccccgatat tttcgttggg tttagggaaa gattctggtc cttggccatt tttatggaga
      901 cgggctgaaa ttcccccatc cagctactgc agcatagctc tctaccacag gaacccaggc
      961 ccccgacaaa tttcgcctca tctctaactc caatttgccg aagttctatt cgggtgcgaa
     1021 aaactgaggc caagtcttta accagttctc taaagtccac ccgggaatct gcaataaagt
     1081 aaaaaataat cttgcttaca tcaaaggtat actccacatt taaaagccgc atttctaggc
     1141 cgtgcttttc tatcttttca gcacatattc taaaggctcg tttttccttt tctttatttt
     1201 caatatgggt aagatgatca tccggaccgg cctttcttac cacagatttt aattcatatt
     1261 ttatttcatc ttctggaaca tccttgggtc ccaaaacaac ttgaccatat tcaagaccac
     1321 gaatggtttc tacaattaca tagtcttctt gtttaaggtc catatccttt ggatcaaaat
     1381 gatagatttt tccgcctttt ttaaagcgaa ctcctataat cttcattgat tcattccttc
     1441 ctgtagctgc agcaaacaac tccaaagtag aatctctgga ttacagtttg cagctagtct
     1501 tctttgcccc ttttctaatt cttctcgaaa gagggggatt ttatttcttc ccaaactctg
     1561 ggaatgacgt ataagaacat caagacgatc ttggtaatat aagttttgtg tattgcggtt
     1621 ttggccaact agggccaaat ccctcaacca aagcatacaa tgctctataa gaagggaggc
     1681 catctgcctt tcttttccat actctctggc ataatctaag agttttaggc ggtttccttc
     1741 ttctagggcg tcaaaaattt tttcaggaaa gaaaaagagt tttcttagct cttcatcttc
     1801 gagtatggca aaggcccgct gaaagctacc cccactaaat ctggcagcaa gacgacgttc
     1861 cttttcttca tatccttcta gagccctata aatttcctct tcgctatggg gtttaagtgg
     1921 atactcgaca acccggcttc gaatggtatc aagaagtctg cccggggccg agcttacaaa
     1981 taaaaaggtt aaaaaatcag gaggctcctc cagggttttt aataatttat tttgggcctg
     2041 agcattcatt ttatgggcat cattaataat aaggactttt cttttcccat aaagaggctt
     2101 ctccacaaca aattgtaaga ccctttttat ttcttctact cctatattgg ttttttcctc
     2161 aataaaaaga aagtctggat aatggtctag ttgctcttgg cttgggatat ctaaaagaaa
     2221 agctgccaac tttctggcta gctttttctt accaattcct tcaattcctg taaaaatcaa
     2281 agcatgggaa agctgccttt tttttgcaag agagcttagc tgcttaaagc tttgatcaaa
     2341 tccaattagt tccattagaa tttctgaaat tcttcgacat caacttggaa gtatatggct
     2401 ccccccaccc ttacttcaat gggttcgctg gcggtaataa taccctgggc aataacaggt
     2461 atactgggca tcatttctac tcgggagcga catttttctc taatcacatc ttttatcagt
     2521 tctacctgat catccttggc accaataaga atggttgtat tgccccggcg taaaaagcct
     2581 ccggagcttg ccagcctggt tactccaatt ttttctttat tgagtctgtt gaccacccta
     2641 tttacatcat cttcttgcat aataataatg agtaatttca ttgagaccct cctatatatt
     2701 ctatacgatt taatggccag tacctaaaaa acacgctgcc tagtatatcc ttctttttta
     2761 caaaacctac ggtagaatct ctagagtcta ggctgttttc tctattgtct cctaggacca
     2821 aataattatc tgccggtagc acaatatcca agtttccctg ggttgtccct tttatatagc
     2881 tttcttcgag ggggacttca tcaatgtaaa tttgtccatc ctctaatcga aatcgttctc
     2941 ctggtaaggc caccactctt tttattaagg tcattgagtc ctctggcttg aggaaaatct
     3001 tttgaatcct gctaagatat tgccagcgac tttcacttag agcaaggcct gagttaaata
     3061 taacgacatc ccccctcttg ggagaagaca tactgtttac tagtagccgg tctccctccc
     3121 tataattggg aagcatggag gtattgacaa ccgatgttgg ttgaataaaa agtagaatca
     3181 aggctaggat ggcaatagag ccagcaaagg ctttaagcca ttctgctaga gcttggccaa
     3241 ggccagcaag tcctttaaaa ttgtttttca tatacacgaa cctttctagc ttgattataa
     3301 cataggaggc cttatgtgag tagactctaa gggctagtct agcaaaatat tgacaaagcc
     3361 ttctttttcc agtccaacaa tatttccttt gaagctttcc agataggcta ctagctcctt
     3421 attcatgatt tctcctggca ctagaatggg acttcccggg ggatagggaa tgatatactc
     3481 actaactacc tctcctaggg cctcctggta ggagcagacc ttagagtctt ttaaaaaggc
     3541 ctcccttatt tccatggcct ttttaggtct aaagacttga tagctgattt tttctttttt
     3601 acggacctgg acctgcttaa gggcagttag cagcctagaa aagtcttccc tagtcgacat
     3661 aatactggga attaaaagca ggccctggtc cgattgaaac tctatttgta ggcccctctt
     3721 gcggagctct ttttccatat gactatagtc tatgggaagt ttgtggcgca gaaggagtct
     3781 tgttttatca tgttttccct ctttgtatat cagcctaaaa tccgtggtct tttctagttg
     3841 gaccttaaag tcctggcagt gtaaaagaag tttttgcatc agctcttttc cctggtagat
     3901 ataaatatct attgcaatat caagacttct cataagcaca tagctgggtg aggtgctttg
     3961 gaacagtttc aagttttttt gcacatagct ttttttaaag cggtcagtac agaggtggag
     4021 gatggccgtc tgggtcatgc tgggcagggt tttatgaaaa gagtggacca ccatatcccc
     4081 atagcgcatg ccagagagcc tatcatcaat cagttttaag tggctgccat gggcctcatc
     4141 tataattata atgagatctt tacttactag ccactcatat acttcatctt ctataatctc
     4201 cccataaaaa ctcggagaag taagaatcac tccccttgta ttttcactga ccagctcctt
     4261 tagctgggct agaaggtccc catcagaaaa tagatatttt gctttaagtc cgagtagata
     4321 gactccatca tagatacaga tatgagaatt acgcataata ataatctcat ctccccggct
     4381 aaaggccgag ctcatggccc ccaaaagagc ccctgtcgct ccattaaccg agataaagct
     4441 acctttactt ccaaaggctt ttgagatttc ttctatggtc tgcaaaataa ttccctgggg
     4501 attggttaaa ttgtctgtgc cgggaatttc agtaaaatca tactggtaga tttgtttttc
     4561 tatcctgccc ttatggcctg gcatctcaaa atgaaccagc tcttcttcta tcactctatc
     4621 caaaccttct cttaatttca tttttttccc ctttcatact ttctcccatt atatcaccat
     4681 cccaggccct agcctttttt tctttcttct cttctttgct ttttacccgg cctttgttat
     4741 aattaatcca ctacaaccat gaggaggaat catgttatca aagagagcat ccaatatttc
     4801 accatcgatt accctgggaa ttagcgccaa agtaaaagaa cttaagagca agggccagcc
     4861 cattatcaac ttaagcattg gtgaaccaga tttttttacc ccagataagg taaaagaaga
     4921 aggaaaactt gccataacaa ataatcttac caagtacgat gcggccagtg gaaatctttc
     4981 cctaagagag gccatatcgg aaaaattaaa aaaagaaaac ttccttagct atcagccaga
     5041 gcaaatcgtt gtatcaagcg gggccaaaca cgccattaca aatgctctgc ttgctaccat
     5101 tgatccgggt gatgaggttc ttattcctat tccctactgg gtaagttatc cgcagatggt
     5161 ttctctagta ggtggtgtgc ccattctggt agctacaaaa aaagaagcgg gctttaaagt
     5221 gagccttgat gatattaggc cctatcttag tgaaaaaaca aagatgatta tcttaaccaa
     5281 tcccagtaat cccacaggaa atgtcttctc caaagaggag ctggaagtcc tttgtgggga
     5341 gctggctaaa aaagatatcc ttatcttagc cgatgagatc tacgagcggg tggtctacga
     5401 tgagcccttt acttccatcc ccagtctttc ccaggagatt tatgataaaa ccatccttat
     5461 taatggccta agtaaatcag cctctatgac cgggtggcga ctgggctata gcgcctgcaa
     5521 taagaccctg gccaaggcca tgggcagcat ccagtcccat cttaccagcc acccctctac
     5581 catcagccag gccgcctcca ttgtggcctt aagagattgc actgaagagg ttgagcatat
     5641 ggttaagacc tataaagagc gcagggacta tattcaaaac ttctttaagg agtggggggt
     5701 gctagaaatc attccaccaa agggtgcctt ttatgtcttt attgatatca gccccttggc
     5761 agccaaactt aaggaggagt cactttctct tagggtctgt gatgatatga ttgaagagca
     5821 taaggtagcc tttattcctg gcattggctt tggggaagac gattttattc gtcttagcta
     5881 tgctgctgct ttagaggaaa taaaagaagg cctagaacgc tttaaaagct atgtggagca
     5941 gatccttgaa gattaaatcc atagaagaaa aacccctaag ctaccttaaa tcatttcata
     6001 taacttacga ggacaaaaaa ggccagccca gaatctggga agtggcctcc cggggcagca
     6061 aggagcgcct ggaaaaagaa atccagggcg agattttctc cgatggagca atgattgtag
     6121 cctgggatga taaaaaagaa catgtcctta tggtgcgaga atttcgtgtt atttcgggcc
     6181 atgacgtaat tagctttccc gctggattaa atgataagga tgaagatatc ctaactacgg
     6241 cggtgcggga gtttaaagaa gaaaccggac ttgatcttct gccagaagga atcgatcggc
     6301 cccgctacac cagtgtaggc ctttctaatg agagggttta tactgttttt ggtcgtttta
     6361 gcggccaaat ctccacggcc taccaggaag atagtgagcg gattctaccc tttctggtcg
     6421 atagaaaaaa agcaatagag ctcctagaaa aagaggatgt gaccattaga agtgctttta
     6481 ttcttcgggg gctcttttct cttcccctat atgaaaaatt gtaaaaaaaa ggtttctgtc
     6541 tgggcagaaa ccttttttta ttccttaagt tttactctaa gttcaacagg attatgatca
     6601 ctattatt
//
LOCUS       DASRNA010000044         4649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_912527,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000044 DASRNA010000000
VERSION     DASRNA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912527"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..544
                     /locus_tag="VFD08_01270"
     CDS             134..544
                     /locus_tag="VFD08_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09675.1"
                     /translation="MKKWFEKFQGKLQRWMYGRYGIDKLSKSLFILSLVFLFLSGRSP
                     IYNLLSLITLALVMIRTYSKDIEKRRRELSLYFDLTRGIRKFWALSKKKFRDRKTHKY
                     YKCPNCKKDFRVPRGKGKIKIFCPNCGHEIIKRT"
     gene            569..1267
                     /locus_tag="VFD08_01275"
     CDS             569..1267
                     /locus_tag="VFD08_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HZK09676.1"
                     /translation="MDKYNEDRLFTEKMKSLADEKTIDWMQKKSSDFIKNMTYYRCAI
                     MEIETKFNVLNEEFSLEHNHNPINTIKTRLKGPLSIKNKLERRGFPLSIDSMTENLND
                     IAGVRVICSFKSDVYLLAQALLSQDDIELIRERDYIKNPKANGYRSLHLLVGIPIFLA
                     HEKHIMKVEIQLRTIAMDFWASLEHQLRYKKEGDFSEEMMGELYECAQSSDALDQRMD
                     NLREKALASPNKKS"
     gene            1458..2402
                     /locus_tag="VFD08_01280"
     CDS             1458..2402
                     /locus_tag="VFD08_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004814080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HZK09677.1"
                     /translation="MLKFMRKIPGGLLIIPMLLSALVNTIAPGFFGQFGGLTEALFTT
                     KGINYIVAMVCFCSANLLDVRSIGKVLKKQGTILLVKVLLCLVFSYIFMQLFSLQGVA
                     GVSAIAFVVAICSVNPSLYLALVSDYGEELDKAAFGLTGLICVPAFPIFVFSITQGGG
                     IDWTPILSTLIPITAGFMVGNLDKELAKMFSSLLVPLTPFMGVAFGAGINIIDSLKAG
                     PQGILITILFYLLLFPIMFTVETKLLKYDGIATLGMSSIAGMSIAVPSILAFSNTDYL
                     AIAPTATAQIAFGVLLTSAITPLITQWYAKKNAILKNI"
     gene            2497..3564
                     /gene="mutY"
                     /locus_tag="VFD08_01285"
     CDS             2497..3564
                     /gene="mutY"
                     /locus_tag="VFD08_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZK09678.1"
                     /translation="MIIILSTKLLDYYDKNKRDLPWRTNPSPYRIWVSEIMLQQTRVG
                     AVIAYYENFLENLPHIEALARADEDLLHKLWEGLGYYSRVRNMKVAANQILKDHGGKL
                     PSTYEELITLQGIGPYTAGAIASMAFNQVQPAIDGNVLRIYSRLHCIEESIDLARVKK
                     KITSLVLEDISKTRPGDFNQALMDVGSGICLPKNPKCQLCPLSDFCKAYKENRQKDLP
                     LRKAKAAKKKEEWTFLILEKEGKFLIQKRPTKGLLAGLWQFIQVPSHLKKKEVKELLE
                     DKNYSPKNIKKLDSNKHIFSHIIWNIRAYLVELEDEFIIMEEDAPYGPFVWASQEEIK
                     EIYPFSSAMDKYVEEVLSWKK"
     gene            complement(3601..4023)
                     /locus_tag="VFD08_01290"
     CDS             complement(3601..4023)
                     /locus_tag="VFD08_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09679.1"
                     /translation="MKYKVVYFTRTGNSRRVAKKISSRLSCEMIEIRDNKSWKGPIGY
                     VRGGFYSFADRGVDISLSKKLGDYDELIVVSSLWAGKIAPTTRKFLKEVPDKKVHLLV
                     TSMGTILKKRPAYKSVSDIVDKKENEDQVITSLLGSLL"
BASE COUNT         1465 a          943 c          833 g         1408 t
ORIGIN      
        1 gttataatac attttgtagg ggaaaaattt ctttttgttt tttttcttat tataggtttt
       61 ttaattccct agcgccctat ataaagttat actagttaag agtaagtttt acataaaagg
      121 agaatgtacc aaaatgaaaa aatggtttga aaagttccaa ggcaagctgc aaagatggat
      181 gtatggaaga tatggcatag acaagctttc aaaaagctta tttatccttt ctttggtttt
      241 tttgtttcta tctggccgct cacctatcta taatctattg tctcttataa ccctagccct
      301 tgtaatgatt cgtacctatt caaaggatat agaaaaaaga agacgggagc tcagcctgta
      361 ttttgatctt acaagaggaa ttagaaagtt ttgggctctt agtaaaaaga agtttagaga
      421 ccgtaaaaca cataaatact acaaatgtcc taattgcaaa aaagattttc gtgtaccaag
      481 gggcaaggga aaaataaaga tattctgccc caattgtggc cacgaaataa taaaacgaac
      541 ataggctcta ttatagagga gaccacttat ggacaaatat aatgaagata gactatttac
      601 tgagaaaatg aaatccctgg ccgatgagaa gaccattgac tggatgcaga aaaaatcaag
      661 tgattttata aaaaacatga cctactatcg ctgcgccatt atggagattg aaacaaagtt
      721 caatgttcta aatgaagagt tttctttaga acataatcat aatcccatta ataccattaa
      781 gacaagactt aaggggccac ttagtataaa aaacaaacta gaaagacgtg gctttcccct
      841 cagcattgat tctatgacag aaaacctaaa tgatattgct ggtgtgcgag ttatctgttc
      901 ttttaaaagt gatgtctacc tactggccca ggccctactt tcacaagatg atattgagtt
      961 aataagagaa agggattata taaaaaatcc caaggccaat ggctaccgca gccttcattt
     1021 attggttgga attcccatat tcctagctca tgaaaaacat attatgaagg ttgaaatcca
     1081 gcttcgcacc attgccatgg atttctgggc cagcctagag catcaactgc gatacaaaaa
     1141 agagggggat ttttccgaag agatgatggg cgagctttat gagtgcgcac agtcaagtga
     1201 cgccctagac cagcgtatgg ataaccttcg ggaaaaggct ctagcttccc caaataaaaa
     1261 atcataataa aagatcttgc cccatcttgc cctacttata gagggtaagt gggcccttaa
     1321 atgaaaaaac acaaatacta ctatttatca ggatccttct tttttcctcg aacagtgata
     1381 tttatctaga agaagataaa agggaaccca aaactatagc caatcatctc aaccctaaaa
     1441 aaatttggag gtagcctatg ctaaagttta tgagaaaaat accaggaggc ctgcttatta
     1501 ttcccatgtt attatccgcc ttggtaaata ctattgcccc tggttttttc ggacaatttg
     1561 gaggcttaac agaagcacta tttacaacaa agggaataaa ttatattgtg gccatggttt
     1621 gcttttgctc tgcaaacctg ctggatgtaa gaagtatagg caaggttctt aaaaaacaag
     1681 gaaccattct acttgtcaag gtacttcttt gtcttgtttt tagctatatt tttatgcagc
     1741 ttttttcttt gcaaggagtt gcaggagttt ctgccatagc ctttgtggta gcaatctgtt
     1801 cggtcaaccc ctccctctac ctggccctag ttagtgatta tggggaggaa ctagataagg
     1861 ccgcctttgg cctgaccggg cttatttgtg ttcctgcctt tcccatattt gtctttagca
     1921 tcacccaggg tggtgggata gactggaccc ctatactctc tactcttatc cccattacag
     1981 cgggctttat ggttggaaat ttagataagg aattagccaa gatgtttagc tccctgctgg
     2041 tgcccttgac cccctttatg ggggttgcct ttggagcagg aatcaacata attgactctt
     2101 taaaagctgg ccctcaagga atccttatca caatactttt ttatctgcta ttatttccta
     2161 ttatgttcac cgtagaaaca aaactcctaa agtacgatgg catagcaaca ttaggtatga
     2221 gctccattgc tggcatgagc attgccgtac ccagcatact tgcctttagc aatactgact
     2281 acctagctat agcccccact gccacagcgc aaattgcctt tggagtcctt cttaccagtg
     2341 ccattactcc ccttattacc cagtggtatg ctaaaaaaaa tgccatccta aaaaacatct
     2401 aaatcagtag ctccctactc ttattataag agtagatttc ttaggagcca cccagagagg
     2461 ataggcccta tccttcttct acctacaatt tttgaggtga ttatcattct ttcaacaaaa
     2521 ttattagact attatgataa aaataaaaga gacttgccct ggaggaccaa tcccagtccc
     2581 taccggattt gggtctctga gattatgctt cagcaaacca gggtcggggc tgttatcgcc
     2641 tactatgaaa actttcttga aaatctcccc catatagaag cccttgccag ggctgatgaa
     2701 gatctacttc acaaactttg ggagggcctg ggctattatt cccgggtaag aaatatgaaa
     2761 gtagccgcca atcaaatcct taaggaccac gggggtaaac tgccaagcac ctacgaggaa
     2821 ttaattaccc tccaaggcat cggtccctat acggccggag ccattgcttc tatggccttt
     2881 aatcaagtcc aacctgctat tgatggaaat gtcctgcgca tctacagtcg ccttcactgc
     2941 attgaggagt ccatcgacct ggccagggtt aaaaagaaga ttactagtct ggttctagaa
     3001 gatatctcaa agactcggcc tggtgacttt aaccaggccc taatggatgt gggcagtggt
     3061 atttgtctac ctaaaaaccc aaaatgccaa ctctgtcctc ttagcgactt ttgtaaggcc
     3121 tataaagaaa ataggcaaaa agacctcccc cttcgaaaag ccaaggctgc taagaaaaaa
     3181 gaagaatgga cttttttgat tttggagaaa gaaggaaagt tcctaattca aaaaagaccc
     3241 actaaaggtc ttttagcagg tctatggcaa tttattcaag ttccttctca tttaaagaaa
     3301 aaagaagtaa aagagctcct agaagataaa aactacagtc ctaaaaacat aaaaaagcta
     3361 gactccaaca agcatatctt tagtcatata atttggaata taagagccta tttagttgag
     3421 ctagaagatg agttcattat catggaagaa gatgccccct atgggccttt tgtctgggct
     3481 agccaggagg aaataaagga aatttatccc ttttcttctg ccatggataa gtatgtggaa
     3541 gaggtgctat catggaagaa ataaagcttt tttcctagtg agcttaacct tctaggcctc
     3601 ctacaataaa ctcccaagaa gactagtaat tacttggtct tcattttctt ttttatcaac
     3661 aatatcactt acagacttat aagcagggcg tttttttaag atcgtcccca tggacgttac
     3721 aagtagatga acttttttat ctggcacttc ctttaaaaac tttctagtag tgggtgcaat
     3781 cttaccagcc catagggatg atacaacaat gagctcatca taatccccta gctttttact
     3841 taagcttata tctactcccc tatctgcaaa agaataaaag cctcctctta catagccgat
     3901 tggtcccttc cagcttttat tgtccctgat ttctatcatt tcacaggaaa gtctggacga
     3961 tatcttcttg gccactcgcc tactatttcc ggtcctagta aagtaaacaa ctttatattt
     4021 catttgacct cctttttctt tctagtaaat ccagccttag tcaaaagctc tcatacatat
     4081 tttcctatag cttaagaact tataataagt ataccctaag atacaaattc taaggctaaa
     4141 aagatagatt cctgcaaata gaatttaact tacgccttag gctttaaaaa aagctgcaac
     4201 aagtatcagg ctcttttaaa tttccatacc aagggaaaga tacaagaagt ctttatagtt
     4261 caaatacagg acccagcttt tctcttgcca aagtggtctt ttattaaaaa aagtcctaca
     4321 tattctgata tatgaaaact ctgaacttgc aattaaaatg ctagaacttg caactaatat
     4381 ctttaaaggg cgctaaaatc atagcttttg ttggttttct acttaagaaa aaactatcac
     4441 tttaatactt actgaaaata aaaattattt tctttaaata taagccctta tagctaaaaa
     4501 aattattttt cacttaatct ttcgaaataa ataagttttc agctactact ttatactttt
     4561 accccttcat tttttctctc acaagggcca gatggggctt tttgtttttt agcagtataa
     4621 agtaactatc taaattttta gattctatc
//
LOCUS       DASRNA010000045        15257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_313843,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000045 DASRNA010000000
VERSION     DASRNA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15257
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313843"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..1187
                     /locus_tag="VFD08_01295"
     CDS             378..1187
                     /locus_tag="VFD08_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK09680.1"
                     /translation="MILQANEGTIKIDKSEMHYVMFGKGHKSLVILPGLSDGLKNVKG
                     ARAMHAMMYKRFAKEYRVYVFSRKNHMEEGYSTRDMARDQRIAMRNLGIENAHIMGFS
                     QGGMIAQWLAIDYPQRVDKLVLAVSLARQNKTIQEVIGKWIELAKINDYKSLIIDALE
                     KTYNEDRLERYRKSYPIITRYGKPNSFENFIVQAYACLNHDAYKELEKIKSPTLVIGG
                     DSDKIAGKASSQELADKLKNSQLILYKGLGHGAFEEAKGFKEEVLEFLSED"
     gene            1473..2690
                     /locus_tag="VFD08_01300"
     CDS             1473..2690
                     /locus_tag="VFD08_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HZK09681.1"
                     /translation="MKKRIALFLALVMMLSLVGCGSPAEEAEAPAETETEEVAEEAEE
                     AEEVEEVEEEEEEEEVVAETDYKIGVMTGTVSQGEEEFRAAENMKEKYGDMIVLSTYP
                     DRFMQEQETTITNMMALASDPDVKAIVMVQAVPGAAAAIGRVKEMRDDILFVLGVPGE
                     DPDVIAEYGDVILQVDDIARGAQIAQQAKEMGAETLVHYSFPRHMSYEMLAARRDNMK
                     AEAEEIGLVWVEQDAPDPTGDAGVPGTQQFIMEDVPRKVEEYGENTAFFGTNCAMMEP
                     LIRQIVETKALFPVQCCPSPYHALPAALGIAVPEDKAGDLNFIIDEIQAKVSEAGMEG
                     RVGTWSVPVNMMFVEAGVDYAIEYLEGNTDGKVDEELLITLMEGIAGGSVELEQYGDY
                     EHYFLYLGASKVF"
     gene            2849..4432
                     /locus_tag="VFD08_01305"
     CDS             2849..4432
                     /locus_tag="VFD08_01305"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZK09682.1"
                     /translation="MRDIGKEYYGTRVLSGINLRLKAGEIHAIIGENGAGKSTLMNIL
                     FGMSVIHSTGGFDGKVFVDGKETFIESPVEAMHNGIGMVHQEFMLIPGYSITENIKLN
                     REITNPGILSKIAGKKFETLDMKAMNKDARKSLDRVGLNVEEWVKVAGLPVSFMQFVE
                     IARELDKENLKVLVFDEPTAVLTESDAERLLDTMQMIADTGIAILFITHRLNEVTRVA
                     DKITVLRDGEHVATKSIKDTDVFDMASLMVGREIQMDEAQSISSEGKAVKMEIKNLFV
                     DMPGEAVKGIDLNIYEGEILGIGGLAGQGKIGLANGIMGLYNSEGSIKLEGQEITLNN
                     PEKSLKEGLAFVSEDRRGIGLLLDNSIELNIAVTSMQIQGKFINKFGPFSQRDNKGIR
                     KHALEMIEDLDIRCTGPDQVTRRLSGGNQQKVCIARALTLSPKLLFVSEPTRGIDVGA
                     KKIVLDTLLDLNKNHGMTIVMISSELAELRMISHRIAIINEGKLEGILPPDASDVDFG
                     LMMSGEYKHVKGKERKTDD"
     gene            4425..5468
                     /locus_tag="VFD08_01310"
     CDS             4425..5468
                     /locus_tag="VFD08_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK09683.1"
                     /translation="MTKTIKKYVDQFGIPRIIIVVFLLTLLVMAVMLEIPINNLLSDA
                     LVRTGMNGILVLAMVPGILAGIGLNFGIPIGIVCGLLGGLLAIEFDLQGFTALFTAVA
                     ISIPLSLVAGYFYGLLLNRVKGSEMTIATYVGFSIVSLMSIGWLVFPFKSPDMRWPIG
                     TGLRTTITLQGRYERILNDWGSFDILGVKIPTGLLLFMLFFCLLVWLFLRSKTGTAMI
                     AAGDSPKFATASGINVNKMRVIGTMLSTMLSAVGIVIYSQSFGFIQLYQAPMFMSFAA
                     VAAILIGGATINKANIANVLIGVFLFQSVLVVSLPVANKILEDVSSLAEISRIVISNG
                     IILYALTQVGGGE"
     gene            5468..6757
                     /locus_tag="VFD08_01315"
     CDS             5468..6757
                     /locus_tag="VFD08_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK09684.1"
                     /translation="MDKNIKITPPKMTLPQRIKKTLTHNMVTVFFIILCLIGLYFSGL
                     SFSFLLGDIITRMIRNSFLVLALLLPVMAGMGLNFAIVLGAMCAQAAVIFVTHWEIAG
                     LPGIGVTVLLATPLAILLGYLTGKLFNKTKGQEMIGGMILGFFSNGIYQLIFLILLGG
                     LIPFYNETLVLSSGVGLRTTIDLAGGLRYAIDDFWRLPFTTAGIGFAVLAILYAGLRY
                     IKGDRKLGEEAGEKKYLFTGAVSIAFIAWLLYIKSSESMINFISVPMLTVLLIALMCI
                     FTTYFIRTKLGQDFRTVGQDRHIANVSGIHADKTRLTAMILSTVFASWGHIIFLQNLG
                     TFSTYGSHEQIGFYSVAALLIGGASVTNAKISHALLGTLLFHTLFIVSPLAGKNLFGD
                     AQLGEYFRVFIAYGVIGVSLVMHAWRKQIENKKRLSE"
     regulatory      6925..6983
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            7036..8211
                     /locus_tag="VFD08_01320"
     CDS             7036..8211
                     /locus_tag="VFD08_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZK09685.1"
                     /translation="MAASLATITLLGLLSHKLFDRFKLPGLLGMMIVGVFIGPYGFDL
                     LSYEIIAASADLRKIALIVILLRAGLGIKKEDIKKMGRPAIYMSFIPGLLEGFAIIYI
                     SMRLFGFTFIQGGILGFIIAAVSPAVIVPSMLDLIEKGKGEEKSIPTLILAGASMDDV
                     FAITIFSTFLGIYSGHLINIKAKILTIPLSIFLGILAGTLIGIILVKIFEKYHIRDTK
                     KMLYILASAILLTRVETMIKSKVEIAGLLGVMAIGFIIFEKAPKLGERMASKFNKVWV
                     FAEILLFVLIGAQVNVALALDAGLKGLVLLAIGLAARSVGVLLSLMGTELNRKEKLFC
                     VLAYLPKATVQAAIGAIPLSLGVESGDLILSLAVLSILITAPLGAVAIKIGGDKLLQ"
     gene            8389..8607
                     /locus_tag="VFD08_01325"
     CDS             8389..8607
                     /locus_tag="VFD08_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09686.1"
                     /translation="MKEFIGKRWGQISDGERAMLMSQAYVNKERIDEKTGGCIVQFES
                     GLKAIGTIRKEEDHIVIDIGKEAKLYID"
     gene            complement(8713..9792)
                     /gene="ychF"
                     /locus_tag="VFD08_01330"
     CDS             complement(8713..9792)
                     /gene="ychF"
                     /locus_tag="VFD08_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZK09687.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITKAGADSANYPFCTIDPNMGVVDV
                     PDPRLEILREINQSERTVPAQVTFFDIAGLVKGASKGEGLGNQFLSHIREVDAILHVV
                     RCFESSNISHVDGSIDPKRDIQTIQLELILADLETLERRQARLERQIKGDRELKKEYD
                     LVLKLISHLEEEKQAKTFSLEKDEIALYKQMQLLTSKPVIYVANVSVEEPKPSILKDI
                     EEIANREDASLLAISVQIEEEIAELPEEDKEAFLKEMGLSISGLDRLIQASYSLLNLI
                     SFITAGPKEIRAWTIHKGTKALDAAAKIHSDIQRGFIRAETIDFDDLIKAGSMLKAKE
                     LGLVRLEGKEYIVKDGDIILFRFNV"
     gene            10368..11558
                     /locus_tag="VFD08_01335"
     CDS             10368..11558
                     /locus_tag="VFD08_01335"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZK09688.1"
                     /translation="MNYTHWIYKEGFTTKLTSCYYILPLSPRSATLGALLARVLERGS
                     KEYPTVEAVNDKMDELYGAAAFFDVNIYGSYLCFEARLIMPKYGFLDQPELEEEAREF
                     LRSLLYRPLLEKGTFKEDFFLQEKEMLAQDIENLYKDPDSYALLRSLELIFPDSNLGI
                     YKYGDSRVLKELSRKDLWDFYQELMQAPLYIYHHSDEKKEDLGKKGFFPMEKVNNKVT
                     ERTCKEDRDVDQSILIQAYFVPWTYQDPMVYPLLVVNHLLGGSGTSLLFKEVREEKGL
                     CYSIYSRYDRFRQVLFIMSGYEKEQGQELVTSIEGVLQALREGNFSDKELADTKQDLC
                     IGIASIPDSQGRHLKDSFVRDLFGDKKDIESRIEEIKKVEKTDVVKAANSLSRLLSFY
                     VRQK"
     gene            11555..12811
                     /locus_tag="VFD08_01340"
     CDS             11555..12811
                     /locus_tag="VFD08_01340"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZK09689.1"
                     /translation="MKKEKIIDTVSRQSYDRYTFSSGFSLVHYPKKGRFLKAAYLSVG
                     FGSTDIQGQIGGKSFELPEGTAHFLEHKLFEGEEDLFEKMSSLGASVNAFTSHDLTCY
                     YFNTPINFQASLDHLLSIPIKPEYTKEGVIKERNIIGHEIKMYQNDVDYFTYHRGLSY
                     LYPNHPVGNDIAGSLESLERIDKEVLDLALENYYVPSNMLLFLIGDFSEKEMEEILEN
                     LPPFYLRKKELAKTIYPEDQIIPSHRQLLSYKDISMASFSYLIKLAAPKSKTIGFEKS
                     IAYTIALDSLFGEGSALYTHHYEKGDLLNLSSDYSYGEGYGVISLSGEGKSPFAIEEG
                     IKKQIIKAQAVDEKKIMRVKRRLMGRYLMGFNSLSGIAGNFTHLHHRGIELFDYLPLV
                     EKTLISPSQELFKGASCFSLTIKEKE"
     gene            12808..13539
                     /gene="lgt"
                     /locus_tag="VFD08_01345"
     CDS             12808..13539
                     /gene="lgt"
                     /locus_tag="VFD08_01345"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZK09690.1"
                     /translation="MNPVAFTFLGLEIHWYAILMVLSFFIAGGLVQRWGKEKGYKEED
                     LLDLTLVVILTAIVGARLYYVAFSWEHYQGDILKIINLRGGGLAIHGGIIGGLIGGYF
                     FCKRRGLNFLELADFTAPGLILGQAIGRWGNFFNQEAYGGPTNLPWAITVAGEKVHPT
                     FLYESLWNLGVFFILLRLLKKKEFDGQIILLHGILYSLGRLWIEGLRTDSLMLGPLRI
                     AQVVSLGTIVLFSGFYFYGIKKKRN"
     gene            13684..14112
                     /gene="mraZ"
                     /locus_tag="VFD08_01350"
     CDS             13684..14112
                     /gene="mraZ"
                     /locus_tag="VFD08_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZK09691.1"
                     /translation="MFIGEFNHTTDAKGRVNIPSIFREDLGESFYITKGLDKSLFVFT
                     QTEWDKFQEKLQDVPLTNKTGRAFTRFFYAGATKCSLDKSGRILIPQNLRQFAQIDKE
                     TLIIGAGSRVEIWSEPVWNQYNEDELSYDELADNMEELGI"
     gene            14114..15034
                     /gene="rsmH"
                     /locus_tag="VFD08_01355"
     CDS             14114..15034
                     /gene="rsmH"
                     /locus_tag="VFD08_01355"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZK09692.1"
                     /translation="MSFSHISVMPKEVIEYLQIKPDGIYLDGTLGGAGHASRVLEQLS
                     KGLLIGIDKDEDALHYSKKKLSALSGNFVLAKADFYNADLLLDELEVDKIQGAILDLG
                     VSSYQLDQAQRGFSYHQDAPLDMRMNQCQKIDAAYIVNEYSEEELSKILWEYGEERWA
                     KRIASFIVDYRPIETTLELVDVIKRAIPAAARESKHPGRKTFQALRIAVNGELDTLDE
                     SLNKIFSRIDKGGRLVVITFHSLEDRIVKKTFAAMQKGCICPPELPVCLCGQEPSGKI
                     ITRKPIQASFKELEFNPRSRSAKLRVIERI"
     gene            15036..>15257
                     /locus_tag="VFD08_01360"
     CDS             15036..>15257
                     /locus_tag="VFD08_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09693.1"
                     /translation="MKLDFGEFDKVYNEDLKQRKIKESKNRKKKPQNRGVFWGSAFII
                     ILAFFVFANIIDGYVELNQVKFENLELAQR"
BASE COUNT         4792 a         2542 c         3407 g         4516 t
ORIGIN      
        1 agtcttggat ggtggggagg tggaaccatc tacggtttag ctatgttgac agagtttaat
       61 ctctgattca cgctactaga gagtagggtt cacggcgaaa ccaattgcct gttggcaggg
      121 gtaaccctga tccacgtata agaaagtggc agaggccgaa agccactttt ctaagactcc
      181 gaacctaaac caataataca attacatgac ggttttatag ttgatccttt tctgcttgac
      241 aagggtcact tcataacagc taaaaagaca gcttatgaaa agaacctaac ttaaagagaa
      301 gacgatatta tctagcaaaa gatgtgagta tataggagct atctatcagt aaatttcctt
      361 gaggaaggag tcaagttatg attttgcagg ccaatgaagg gaccataaaa attgataaaa
      421 gtgagatgca ctatgtaatg tttggcaaag gccataagtc tcttgtgatt ttgccgggtt
      481 taagcgatgg tcttaaaaat gtaaaagggg caagagccat gcatgccatg atgtataagc
      541 gctttgccaa agaatacagg gtctatgtct ttagcagaaa aaaccatatg gaagagggct
      601 actcaaccag ggatatggca agagatcagc ggattgcaat gagaaatctt ggtatagaaa
      661 acgctcatat aatgggcttt tcgcaaggag gcatgattgc ccagtggctt gctatagatt
      721 atcctcaaag ggtagataag ctggttctag cggtttctct tgccaggcaa aacaagacta
      781 tacaagaggt aataggaaag tggatagaac tagccaaaat caatgactat aaaagcctaa
      841 tcattgatgc tcttgaaaaa acctataatg aggatagact agagcgttac agaaaatctt
      901 atcctattat taccaggtat ggcaaaccaa attcctttga aaactttatt gtacaagctt
      961 atgcctgcct caatcatgat gcctataagg agcttgagaa gattaaaagt cccacattag
     1021 ttataggggg cgatagtgat aaaattgcag gaaaagcttc cagtcaggaa ctagcagata
     1081 agcttaagaa tagccaactt attttgtata aaggacttgg tcatggagcc tttgaagaag
     1141 ccaaaggctt taaagaagag gtcttagagt ttctatcaga ggattagctt aaagtcaaaa
     1201 acattgagat cattgccagg tggctataat gggaaagttt cctaattatg atagaggaag
     1261 aacaaaggga aaggatgaca gataatcgaa aattcttgca ttttattcat aatactagta
     1321 taatccctta taggtaggga cagttattct gtctgtacta gctagtggag gaattagttt
     1381 ggacgtttat tatccaaatg attctgaaag ggggaagaaa agcaagacgg attataagaa
     1441 acaaaataac aaaaaacttt aggaggaatt gaatgaagaa acgtatagca ttattcttgg
     1501 cattagtaat gatgttaagt ttagtaggat gtggttctcc tgcagaagaa gcagaagcac
     1561 ccgcagagac agaaacggaa gaagtagctg aagaagctga agaagccgaa gaagtcgaag
     1621 aagtcgaaga agaggaagag gaagaggaag ttgttgcaga aaccgattat aaaatcggtg
     1681 ttatgactgg aacagtatcc caaggtgaag aagaatttag agctgcagaa aatatgaagg
     1741 aaaaatatgg agatatgatt gttttatcta catatcctga tagatttatg caggagcaag
     1801 aaactaccat aacaaatatg atggcactgg cttcagaccc agatgtcaaa gcaattgtaa
     1861 tggtacaggc tgtacctggt gcagctgcag caattggtag agtcaaagaa atgagagacg
     1921 acatactctt tgtacttgga gttcctggcg aagacccaga tgtaattgct gaatatggcg
     1981 atgtaattct acaagtagat gatattgctc gtggagcgca aattgctcag caggcaaaag
     2041 aaatgggagc agaaacactt gttcactact ctttcccccg tcatatgtct tatgaaatgc
     2101 tagctgctag aagagataat atgaaggcag aagcagaaga aattggtctt gtatgggttg
     2161 agcaagatgc accagaccca acaggagacg ctggagttcc tggaacacag cagtttataa
     2221 tggaagatgt tccacgtaaa gtagaagaat acggtgaaaa cacagcattc tttggaacaa
     2281 actgtgcgat gatggagcct ctaattcgtc aaatcgtaga aacaaaagcc ttattccctg
     2341 tacagtgctg tccatcccct tatcacgcac tgccagcagc cttaggcatt gcagtaccag
     2401 aagacaaagc gggagatctt aatttcatca ttgatgaaat ccaagcaaaa gtttctgaag
     2461 ctggtatgga aggaagagtt ggtacatggt cagttcctgt aaacatgatg tttgttgaag
     2521 caggcgttga ttatgctatt gaatatcttg aaggaaacac agatggcaag gtagacgaag
     2581 agcttcttat tacactaatg gaaggaattg ccggtggaag cgttgaactt gaacaatatg
     2641 gcgattatga gcactacttc ctatatttag gagcgtctaa agtattttaa taactcgaat
     2701 agataaactt agagagttaa aaatatagaa tgataaagta ttgtgcaagg cgaaggcctt
     2761 gcacaatatt gtattaatcc ctgaaattca cacagaagga gggagtgatt atttgaaaaa
     2821 tgaaaataag gatcaatatg tcctcgatat gagggatatt ggcaaagaat attatggcac
     2881 cagggtttta tcaggcatta atcttagatt aaaggctgga gagattcacg ccatcattgg
     2941 cgagaatggt gcgggaaagt caacccttat gaatattcta tttggaatgt cagtgataca
     3001 tagtacaggc ggttttgatg gaaaagtctt tgtagatgga aaagaaacat ttattgagtc
     3061 accagtagaa gctatgcata atggcattgg catggtccac caggagttta tgttaattcc
     3121 aggctacagt attactgaga acattaaact aaatagagaa attaccaatc caggaatact
     3181 tagtaaaata gcaggaaaaa agtttgaaac tcttgatatg aaggctatga ataaggatgc
     3241 aagaaaatcc ttggaccgag taggactaaa tgtagaagaa tgggttaaag ttgctggcct
     3301 tcccgtaagc tttatgcaat ttgttgaaat agcaagggag ctggacaagg aaaatctaaa
     3361 agtactagtc tttgatgagc ccacagcagt tttaacagaa agtgatgctg agagattatt
     3421 agataccatg caaatgattg cagatacagg cattgcgatt ttatttatca cccatagact
     3481 caatgaagta acacgtgttg cagataaaat cactgtactg cgtgatgggg agcatgtagc
     3541 aacaaaaagc atcaaagata ccgatgtatt tgatatggcc agcctgatgg ttggtagaga
     3601 aatacaaatg gatgaggctc aatctatctc atctgaagga aaagctgtaa aaatggagat
     3661 taaaaatctc tttgttgata tgcctggtga agcggtcaaa ggcattgatt taaatatcta
     3721 tgaaggtgaa attttaggaa ttggtggcct ggccggtcaa gggaaaatag gactagccaa
     3781 tgggattatg ggtttatata actctgaagg aagcattaaa cttgaggggc aggaaattac
     3841 ccttaacaat ccggagaaat ctcttaaaga gggtttggcc tttgtatccg aagaccgaag
     3901 aggaattggt cttcttttag ataattctat tgaactaaat atagcggtta cttctatgca
     3961 gattcaaggg aagtttatta ataaatttgg acctttttca caaagggata acaaagggat
     4021 tagaaaacat gctcttgaga tgatagagga tctggacatt agatgtactg gaccagatca
     4081 agttacaaga cgccttagtg ggggaaacca gcaaaaagtt tgtattgcta gagccttaac
     4141 tctcagtcca aaattattgt ttgtatcaga acccacaaga ggaattgatg ttggagctaa
     4201 aaaaatagtt ttagatacct tgctcgattt aaataaaaac catggtatga caattgttat
     4261 gatatctagt gaactagcag agttgcgaat gatttcccat agaattgcca ttataaatga
     4321 aggcaaactc gagggaatct tgccgccaga tgctagtgat gtagattttg gtcttatgat
     4381 gtccggcgag tataaacatg tcaaagggaa agagaggaaa acagatgact aaaacgatta
     4441 agaagtacgt tgaccaattt ggaattcctc gaattatcat tgttgtattc ctgctgacct
     4501 tgttagtaat ggcagttatg ctcgaaatac caataaacaa tcttctttcc gatgcgcttg
     4561 tgcgtacagg aatgaatgga attcttgttc ttgcaatggt tcctggaatc ttagctggta
     4621 taggcttaaa ctttggaata ccaataggca tagtttgtgg acttttaggt ggtctattgg
     4681 caatagagtt cgatttacag ggctttactg ctctttttac cgcagttgca attagcatcc
     4741 ccttgtcact tgttgcagga tatttttatg ggcttttgtt aaacagagtt aaaggatctg
     4801 aaatgacaat agctacctat gtgggatttt caatagtttc actaatgagc atagggtggc
     4861 tggtatttcc ctttaaaagt ccagatatga gatggcccat tggaacgggt ttaaggacaa
     4921 ccattaccct tcaaggcaga tatgaacgga tacttaatga ctggggatca tttgatatcc
     4981 tcggtgtaaa aatacctaca ggattacttt tatttatgct ctttttctgc ttactggtat
     5041 ggctattttt acgatcaaaa actgggactg ctatgatcgc agcaggcgat agtcctaaat
     5101 ttgcaacagc atcgggaatt aatgtaaata agatgcgtgt aataggtaca atgctttcaa
     5161 ccatgttaag tgctgttgga atcgtaatat acagtcagag ctttggcttt atacaattgt
     5221 atcaggcgcc tatgtttatg agctttgcag ctgttgcagc tattcttatt ggtggtgcta
     5281 ccattaataa ggccaatatt gccaatgtgc taataggagt tttccttttc cagtcagtgc
     5341 ttgttgtatc tcttccagta gcaaataaaa tacttgaaga tgtaagtagt ttggctgaaa
     5401 tttctcgtat cgtaataagt aatgggatca ttttgtatgc actcacacaa gttggaggtg
     5461 gagaataatg gataagaata tcaaaattac cccaccaaag atgactttgc cccagagaat
     5521 taaaaaaaca ttaacccaca atatggtaac agtgtttttt atcatacttt gcttaatagg
     5581 tttgtacttc tctggtctaa gctttagttt tttactgggt gatattatta cacggatgat
     5641 cagaaatagt ttcttagttt tagcccttct tttaccagtt atggcaggaa tggggcttaa
     5701 ctttgccatt gtacttggtg cgatgtgtgc acaagcagca gtaatatttg ttacccactg
     5761 ggaaatagca ggacttccag ggattggtgt aacggtactt ctggcaactc ctcttgctat
     5821 actcttagga tacctaacag gcaagttgtt taataagacc aaggggcagg aaatgatagg
     5881 tggtatgatt ctgggcttct tttcaaacgg aatttaccag ctaatattct taatcttgct
     5941 agggggactt ataccctttt acaatgaaac cctggtacta agttcaggtg ttggtttaag
     6001 aacaaccatt gacttggctg gagggcttcg atacgcaatt gatgatttct ggagacttcc
     6061 ctttacaaca gcaggcatag gatttgcagt tttggcaatc ctctatgcag gtctaagata
     6121 tataaaaggg gataggaagt taggcgagga agctggcgag aaaaaatatc ttttcacagg
     6181 tgccgtaagc atcgccttca tcgcttggtt gctctatatt aaatcaagtg agtctatgat
     6241 taactttatt tcagtgccta tgttaacagt tttgcttatt gccctgatgt gtatttttac
     6301 gacctacttt attaggacca agctaggaca agactttaga actgttggcc aagatagaca
     6361 tattgcaaat gtatcaggca tacatgccga taagacaagg ctaacagcta tgatcttatc
     6421 tactgttttt gcttcctggg gacatattat tttccttcag aatttgggaa cctttagtac
     6481 ctatggtagt cacgagcaga ttggttttta ttctgttgca gccctgctaa ttgggggagc
     6541 ttcagtaacc aatgcaaaaa tcagtcatgc tttactggga accttattat tccacaccct
     6601 atttatcgtt tctcccctgg cgggcaaaaa cctatttgga gacgcccagc taggagaata
     6661 cttccgagta tttatagcct atggagttat cggtgtttcc cttgtaatgc acgcttggcg
     6721 aaagcagatt gaaaataaaa aacggctaag tgagtaaaat atacttgttt gaagtttaga
     6781 ggccccagga ttatcctggg gccttttgtc ttatagaaag tgttcttagc cataaaacca
     6841 agagtccagt aagtaagcag aggccatatt gaaaaggagc tggcaaagag gcctaacttt
     6901 attttatctt gtgctataat ggataaaacg ataggtcatg gggttgacct gaaccgcgta
     6961 aagctaatga ctcctataaa ggcacatctt tgcatgtgct tttatgggag tttattttat
     7021 ttaaggagtt attttatggc agcaagttta gcaacaataa cattactagg actcttatcg
     7081 cataagctct ttgatagatt taaactgcct ggcctgctgg ggatgatgat agtgggtgtc
     7141 tttattggtc cctatggatt tgacctactt agttatgaaa ttatagccgc atcagcagat
     7201 ctaagaaaaa tcgctttaat tgtaatcctg cttagggcgg gactgggaat aaaaaaagaa
     7261 gacattaaaa agatgggacg cccagcaatc tatatgagct ttatcccagg gctactagaa
     7321 ggctttgcca ttatatatat atccatgaga ctttttggtt ttacctttat tcagggaggg
     7381 atactaggat ttattatagc agctgtttca ccagcagtaa ttgtgccctc catgctggat
     7441 ttgatagaaa aaggaaaggg agaagaaaag agtatcccaa ccctaatact agcaggagcc
     7501 tctatggatg atgtatttgc cattaccatc ttttcgacct ttttgggcat ctatagtggt
     7561 catcttatca atataaaggc aaaaattctt acgattcccc tttctatttt cctaggaatc
     7621 ctagctggga cacttatagg aatcatctta gtcaaaatat ttgaaaaata ccatatcaga
     7681 gatacaaaga agatgctcta tatactggct tcggcgattt tattaacaag ggtggaaaca
     7741 atgatcaaat caaaagtaga aatagctgga ctgctaggag taatggcaat tggctttatt
     7801 atttttgaaa aggcgcccaa gctaggagaa aggatggcct caaagtttaa taaagtttgg
     7861 gtgtttgctg agatactact atttgtatta ataggagccc aggtaaatgt ggccctagcc
     7921 cttgatgctg gcttaaaagg actagtccta ctggccatag gactcgcagc aagaagtgta
     7981 ggggtgcttt tatctcttat gggaacagag ttaaatagaa aagaaaaact tttttgtgtc
     8041 ttggcctatc tgccaaaggc tactgttcaa gcagccattg gagcaatccc cctatctctg
     8101 ggtgtggagt caggagattt aatcctttct ttggcagtcc tatctattct tataacagct
     8161 ccccttggtg cagtggccat taaaataggg ggagataaac tattgcaata aaaagttttt
     8221 acctggccaa gctagcttaa attttgaaat agctttaaga aggctaaaga ggcaaactag
     8281 ggcttatttt atctattttt cactttattt tgcttttagc gtttaaaatg agtcgaagta
     8341 tagaataata gtaataggaa ggcagctagc ttaagaagga gggaagacat gaaggaattt
     8401 attgggaaaa gatggggtca gatctccgat ggggagagag caatgctaat gagtcaagcc
     8461 tatgtaaata aggaaagaat tgatgaaaaa acaggtggct gcattgtgca atttgaaagt
     8521 ggactaaagg caatcggaac aattagaaaa gaagaagatc atattgtgat tgatattgga
     8581 aaagaagcta aactttatat cgactaaaaa agaaatagat gattgccctg gaagacttag
     8641 aaagcaaaag gactccttct agaaaagagg agtcctgttt ttttcttttt ttatgagtga
     8701 taagggctaa tactatacat tgaagcgaaa aagtataatg tccccatctt ttacgatata
     8761 ttcttttcct tctaagcgaa caagccccag ttcttttgcc ttgagcatgg atcctgcttt
     8821 aataagatca tcaaagtcaa tggtctcagc acgaataaat cccctttgga tatcggagtg
     8881 gattttcgcg gcggcatcta gggcctttgt gcccttatga atggtccaag cacgaatctc
     8941 cttgggtcca gctgtgataa agctaataag atttaaaaga gagtagctgg cctggattaa
     9001 acgatcaagt ccagaaatac ttagccccat ttcttttaaa aaggcttcct tatcttcttc
     9061 aggaagttca gcaatttctt cttcaatttg aacggagata gccagtagag atgcatcttc
     9121 tctatttgca atttcttcaa tatcctttag gatggagggc tttggttctt ctacggaaac
     9181 attggctaca tatatcacag gctttgaggt aagaagttgc atctgcttgt aaagagctat
     9241 ttcatctttt tccagtgaaa aagtttttgc ttgtttttcc tcttcaagat gagagattaa
     9301 ctttaaaaca agatcatact ctttttttag ctcacggtct cccttgattt gacgttctag
     9361 gcgggcttgt ctacgttcta gggtttccag gtccgctaaa ataagttcta gttggatggt
     9421 ttggatatct cttttaggat ctatgcttcc atcaacatgg cttatattgc tgctttcaaa
     9481 gcagcgcact acatggagga ttgcatcgac ttcccttata tgagaaagaa actgatttcc
     9541 cagtccctcg cccttggagg ctccttttac caggccggcg atatcaaaga aggtcacctg
     9601 ggcaggtacg gttctttcag actgatttat ttcacgcagg atctccagcc tagggtcagg
     9661 cacatccacg acgcccatat tgggatcaat ggtgcaaaag ggatagttgg cactatctgc
     9721 ccctgctttt gttattgcat taaaaagggt gcttttacca acattgggaa gccccacaat
     9781 tcctagtttc atagttaata taactccttt gttatttata gataaggggt ttgccagcat
     9841 agatgggaag atagaatacg ttaagatgta gtcccttatt tgctagatac tgtaaaccac
     9901 tatattttat tttgttttag cttgtttctt atcgtatata agtggctcat tggtaaaaag
     9961 agagcctctg gttacgctta caagctcaaa cgagtatttt tctttctttg tttatttact
    10021 tgcaacttgg taaaaagacg acaagcaaaa aaaatagccc cagggaaggt atgatttcaa
    10081 gtattcaacc ggggggaaat ttttgcacac acttaccttt tgcaagatga aatcatttta
    10141 tattctactt cttaccatgg taagatattt tgcctgaaaa gtcaatgaaa taggcctaga
    10201 gtcaatttgt ttttttatcc ttcctttttt tgtccataca aggattattt taagtttttc
    10261 cttgaaaaac cgggacccat cataggagag gaaggctact tgctggttaa aagtagagct
    10321 agatttaatt ttacttttat cgtgctataa ttgtccgagg aggattaatg aattatacac
    10381 actggattta taaagaaggt tttacgacaa aactaacttc ttgttattat atattgccct
    10441 taagccccag gtcggcaacc ctgggggcct tattagccag ggttcttgaa aggggaagca
    10501 aggagtatcc tacggtagag gccgttaatg ataaaatgga tgaactctat ggggcggctg
    10561 ctttttttga tgtaaatatc tatggcagct atttatgctt tgaggccagg ctcattatgc
    10621 caaagtatgg ttttttagac cagcctgaac ttgaggagga agcgcgtgaa tttttacgaa
    10681 gcttgctcta tagacccttg ctggaaaaag gaacttttaa agaagacttc tttttacaag
    10741 aaaaagaaat gctggctcaa gacattgaaa atctatataa agatcctgat agctatgctc
    10801 ttttgcgtag cttagaatta atttttcccg acagtaattt gggtatctat aaatatggcg
    10861 acagtagagt gttaaaggaa cttagtagga aagatctttg ggacttttac caagagctta
    10921 tgcaggcccc cctctacatt taccaccaca gtgatgaaaa gaaagaggat ttaggaaaaa
    10981 aaggcttttt tcctatggaa aaagtaaata ataaagtgac agaaaggacc tgtaaagagg
    11041 atagagatgt tgatcaaagt attcttatac aagcctattt tgtgccttgg acctatcaag
    11101 accctatggt ttatccccta ctagtggtca atcatctgct gggtggctcg ggaaccagtc
    11161 tgctttttaa agaagtgagg gaagaaaagg ggctttgtta ttccatctac tctagatatg
    11221 atcgctttag acaagtcctc tttataatga gtggctatga aaaagagcag ggccaagaac
    11281 ttgtaactag cattgagggg gttttacaag cccttaggga aggcaacttc tcagacaaag
    11341 aacttgctga taccaagcaa gatctttgca tagggatagc ctctataccc gatagtcagg
    11401 ggcgtcattt aaaagatagc tttgttagag atctttttgg tgataaaaaa gatattgaaa
    11461 gtcgcatcga ggaaataaaa aaagtggaaa aaacagatgt agttaaggcg gcaaatagct
    11521 tgtcgcgctt attgagtttt tatgtgaggc aaaagtgaaa aaagagaaaa ttattgatac
    11581 tgtaagtagg cagagctatg atagatacac cttttccagt ggatttagcc tggtgcatta
    11641 tcctaaaaaa ggccggtttt taaaggcggc ctatttaagt gtgggttttg gctcaacaga
    11701 tatccaggga caaataggcg ggaagtcttt tgaactccct gaggggacgg cgcatttttt
    11761 agagcataaa ctctttgaag gggaagaaga cctttttgaa aaaatgagta gtttgggcgc
    11821 ttcagtaaat gcctttacct cccatgatct tacctgctat tattttaata cgcccataaa
    11881 ctttcaggcc tccttggacc accttttatc cattcctata aaaccagaat ataccaagga
    11941 gggggtaatc aaggaaagaa acattattgg ccatgagatt aagatgtatc aaaatgatgt
    12001 agactacttc acctatcata gaggactaag ctacctttat cccaatcatc ccgtgggtaa
    12061 tgatattgcg ggaagtttgg agagtttaga gcgcatcgat aaggaggtct tggaccttgc
    12121 cttagagaat tattatgtgc cctcaaatat gcttcttttt cttattggcg attttagtga
    12181 aaaagaaatg gaggagatcc ttgaaaatct tcctcccttc tatttaagaa aaaaagagtt
    12241 ggctaagact atctatccag aggaccaaat tataccatcc cataggcagc ttttatccta
    12301 taaggatatt agtatggcca gtttttccta tttgataaaa ctagctgctc caaaaagcaa
    12361 gacaatagga tttgaaaaat caattgccta taccatcgcc ctagactccc tttttggaga
    12421 aggcagcgcc ctctacaccc atcactatga aaaaggagac cttcttaatc tttcatcaga
    12481 ttatagttat ggggagggat atggtgtgat tagtttaagc ggggaaggaa aatccccctt
    12541 tgccatagaa gaagggataa aaaagcaaat cattaaagcc caggcagtag atgaaaagaa
    12601 gattatgcgt gttaaaagaa ggctaatggg tagatacctt atgggattta attccctatc
    12661 tggcattgcc ggcaatttta ctcatctaca tcatcggggc atagagctct ttgattatct
    12721 gcccttggtg gaaaaaacac tgatttcccc tagccaggaa cttttcaagg gggccagttg
    12781 ttttagttta acgattaagg agaaggaatg aatcctgtag catttacatt tttaggactg
    12841 gaaatacatt ggtatgcaat tttaatggtg cttagttttt ttatagcagg tggccttgta
    12901 caacgctggg gaaaagaaaa gggatataaa gaagaggatc ttttagatct tactctagtg
    12961 gtaatactta cagccattgt aggagcacgt ctttattatg tggcctttag ctgggaacat
    13021 taccagggtg atattttaaa gattattaat cttcgtggtg gtggcctggc tattcacgga
    13081 ggaattattg gaggtcttat tggaggatac tttttttgta agagaagggg ccttaatttt
    13141 ttagaattgg cagatttcac tgcgccaggg cttattttag gtcaagcaat tggccgctgg
    13201 ggaaactttt ttaatcagga ggcctatggt ggcccaacaa atcttccctg ggctattact
    13261 gttgctggag agaaggttca tccaactttt ttatatgagt ctctttggaa tctgggagtg
    13321 ttttttatct tacttcgcct acttaagaag aaggaatttg acggccaaat tattttgctc
    13381 catggaattc tttattcttt aggtagatta tggattgagg gtctgcgcac agactccttg
    13441 atgctggggc ctttgaggat agcacaagtg gtaagtttag gaacgattgt tcttttttct
    13501 ggcttttatt tttatgggat taagaaaaaa agaaattaga ggacctttca ctagattaat
    13561 cgaaaacact tagatgaatt tgcaaataaa ccttgcagat tctttttttt tcgtgtatta
    13621 ttacattacg ggtggtggaa agtggcacca agtgggagga aatcccaaga ggagactttg
    13681 gaaatgttta ttggtgaatt taatcataca accgatgcca aggggcgggt aaatatacct
    13741 agtattttta gggaagactt aggtgagagc ttttatataa caaaaggtct tgataagtct
    13801 ctatttgttt ttactcaaac tgaatgggac aaattccaag aaaagcttca agatgtgcct
    13861 cttactaata agacaggaag agcctttact cgctttttct atgcgggagc aacaaaatgt
    13921 tccttagata aatcgggtcg aattcttatt ccacaaaacc taagacaatt tgctcaaata
    13981 gataaagaaa ccttaattat aggagcggga tccagagttg aaatttggtc tgagccagtt
    14041 tggaatcagt acaatgaaga cgagctaagc tatgacgaac tcgctgacaa tatggaagaa
    14101 ttgggaattt gatatgtcat tttctcatat aagtgtaatg cccaaagagg taattgaata
    14161 cttacaaatt aagcccgatg ggatttattt agatggaaca ctagggggag caggtcatgc
    14221 ttctcgagtg ctagaacaac taagtaaagg acttcttatt ggaatagata aagatgagga
    14281 tgcccttcat tacagcaaga aaaaattaag tgccctcagt ggaaattttg ttcttgctaa
    14341 agcagatttc tataatgctg atcttcttct tgatgaactt gaggtggata aaattcaagg
    14401 cgctattttg gaccttggcg tttcgagtta tcagcttgat caagcgcaaa ggggttttag
    14461 ctatcatcaa gatgcaccct tagatatgcg aatgaatcag tgccaaaaaa ttgatgcggc
    14521 ttatattgtt aacgagtact ctgaagaaga actaagcaaa atactctggg agtatggaga
    14581 agaaagatgg gccaaaagaa ttgcttcgtt tatcgttgat tatagaccca ttgaaacaac
    14641 tcttgagttg gtggatgtaa ttaaaagagc aattcctgca gcagctcgag aatcaaagca
    14701 tcctggccga aaaacttttc aggcccttcg cattgctgtt aatggcgagc tggatacact
    14761 cgatgaaagt ttaaataaga ttttttcaag aatagataaa ggaggaaggt tagttgttat
    14821 aacatttcac tccttagaag accggattgt aaaaaagact tttgctgcca tgcaaaaagg
    14881 atgtatatgt cctccggaat tacctgtctg cctttgtgga caggaaccta gtggaaaaat
    14941 cattaccaga aaacccattc aagccagttt taaagaactg gaatttaatc ctcgtagccg
    15001 cagtgcaaaa ctgcgtgtga tagaaaggat ttgaaatgaa gctagatttt ggtgaatttg
    15061 ataaagtata taatgaggat ttaaaacagc gcaaaatcaa agaaagcaaa aaccgcaaaa
    15121 aaaagccaca aaaccggggg gttttttggg ggtcggcctt tattattatt cttgcatttt
    15181 ttgtttttgc caatataatt gatggctatg tagaattaaa tcaagtgaaa tttgaaaatc
    15241 tcgaattagc ccaacgc
//
LOCUS       DASRNA010000046         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_161302,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000046 DASRNA010000000
VERSION     DASRNA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161302"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFD08_01365"
     CDS             <1..494
                     /locus_tag="VFD08_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZK09694.1"
                     /translation="AEHGLTYDDLAKADYLSFSEATDNMRNKQIDAAFITSAIPTSSV
                     SELSQTVPIVIISVEEDKINSLAQKYPFYTAVTVPAGSYKGQEEDALSPAVMAMLIVP
                     KDMDDDLVYNIVKNLYDRQQIIVDTHKRGEDITLETALDGMSINLHPGAQKYFDEMGI
                     ESN"
     gene            607..984
                     /locus_tag="VFD08_01370"
     CDS             607..984
                     /locus_tag="VFD08_01370"
                     /inference="COORDINATES: protein motif:HMM:NF020479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HZK09695.1"
                     /translation="MDLFLAKDGFSISYTHSVERTQVIEKYRFENDGFVLSDTWFHSH
                     GAGLPSSTDYDFETSSEGFHIYNINQYFEEVIYRSGKKIADHHLLMGEKKYPFTEFTR
                     PGSAVLFRVEKVPYWKLFLGGVH"
     gene            981..3017
                     /locus_tag="VFD08_01375"
     CDS             981..3017
                     /locus_tag="VFD08_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HZK09696.1"
                     /translation="MTKKEKKRKQTPAEKKEIELVDSGKALEDVDELLEKFDKESSKL
                     RKMGKPYSLIVAVIAISMSLFHLYTGGFGTLLAVKQRSLHLIFTMVLGFMLYPAFGKS
                     EKSRPSIMDFILIILAIVVFGYLFMNVEAIVLKGGKLTKVDFYFGLIAILLTLELTRR
                     VVGPELPLVAIIFLLYAYFGRSVPGILSHRGFSLNRIISHMYFTTEGILGIPLGVSAT
                     FVFMFILFGSFLDKTGVGKFFIDIAYALTGGMKSGPAMTSVVSSGLMGSISGSSVANT
                     VTTGAFTIPLMKRTGYEPHFAGAVEATASTGGQIMPPVMGAAAFIMADFTGIPYIHIV
                     LAALIPALLYYVAVGVMVHLEASKLGLKGIPKDQLPSVPNILLESGYLMVPLMVVVYM
                     LVKQYTPTRSAFVAILVAILVAYIAALIKKDKSFNYKSVLSALENGAKGALGVACACA
                     CAGMIVGVVTLTGFGLRVAEIIVTVARGNLMATLFLTMIASLILGMGLPTTAKYIVLA
                     TMAVPALTKLGVNLMAAHLFILYFGVVADVTPPVALAAYAGAGIAGSNSMKTGVQAFK
                     LALAGFTVPFIFAIDSHLILVESVVGTTVTFIPFYQALPTIATAILGIFCLATAVAGY
                     MLTTSKFYERIILFVSALMLLKPGYLTDGLGLGVLIIIYLVQKTRQKRETLAGL"
     gene            3102..3635
                     /locus_tag="VFD08_01380"
     CDS             3102..3635
                     /locus_tag="VFD08_01380"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZK09697.1"
                     /translation="MSKIYTKGGDKGGTSLYDQVRVSKDDVRVESYGTVDELGAVLGL
                     AKNFVEDEQMRETISAIQHKLFTVASNLATQDQQKVAAKIHEEDILQLEKIVDKYMDA
                     VQRKDCFIVNGSGSRSAFLHVARTVCRRAERRAVSLSGLAEVDPLVIKYLNRLSDAIY
                     AMARFNEDKEECVDYSK"
     gene            complement(3691..4926)
                     /locus_tag="VFD08_01385"
     CDS             complement(3691..4926)
                     /locus_tag="VFD08_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK09698.1"
                     /translation="MDTLWTRDFKLIFSGTVISAMAGQAMQLPLSLMTFANTGSLLKS
                     SYLMILAMLPNLILPLLVSPFVDRAPKKYIIVALDYLMALTFFFFAYLTRGGFVYSHY
                     LALAFLVGSNSAVYMIAYQAWYPLLIPKGHMQKGYAVSSTLYPTIMMVMAPISAFMYK
                     SIPMHYLFILVGVLCTLAATAETFIDNQGKTPGHDMDFTAFFKDMKEAVTYLKKEKGL
                     LNIYSYMSLSSGVAMAGQLYIQVFFQKSLILGVTKLGFLTTAETLGRALGGLVQYKVN
                     IPPKKRFPITQFVYYCYDILDIMLLFLCYPLMLINRFVAGSLGVTSATLRETSVQAYL
                     PPRLRAKVLAFAQMASSFSMVIFQFFGGWLGDLIGPRKSMVILSFFLLLMIFVFITLP
                     QATNKKVYTATDEDALRAF"
BASE COUNT         1445 a          938 c         1118 g         1481 t
ORIGIN      
        1 ttgcagagca tggtcttaca tatgatgact tggccaaggc tgattatcta tcctttagtg
       61 aagcaacaga taatatgaga aacaagcaga tcgatgctgc ttttattaca tcagctattc
      121 caacatcttc tgtttccgaa ctatctcaaa cagtgccaat tgttataatt agtgtggaag
      181 aagataagat aaatagcctt gcgcagaaat atccttttta cacagcagta acagttccag
      241 ctggaagtta taaaggacaa gaagaagatg ctctttctcc agcggttatg gctatgctta
      301 ttgtgcctaa agatatggac gatgatttag tttataatat agtaaaaaac ctatatgaca
      361 gacaacaaat tattgttgac acccataagc gtggggaaga tattacctta gaaactgctc
      421 tagatggtat gagtattaac ctgcatcctg gtgcccagaa atactttgat gaaatgggca
      481 tcgaaagcaa ttaattattt acgaggaaag aaattcattt ctttcctcct tatactactt
      541 ttgtgcctgc tttttatacc cttaaaaaaa tgcctaatcc taaccgatct ttttacgggg
      601 gagtgtttgg atcttttttt agccaaggat gggttttcca tttcttatac tcattctgta
      661 gagcgcaccc aggtgattga aaaataccgt tttgaaaatg acggctttgt tttaagcgat
      721 acctggtttc attctcatgg cgcgggtctt ccttctagta cagactatga ttttgagacc
      781 agctctgaag gttttcatat ctataatata aaccagtact ttgaggaggt catttaccga
      841 tcaggtaaga aaattgcaga ccatcatctc cttatgggag aaaaaaaata cccgtttact
      901 gaatttacca ggcctggctc agcggttctt tttcgtgtag aaaaagtacc ctattggaaa
      961 ctttttttag gaggtgtcca ttgacaaaaa aagaaaagaa aagaaaacag acgccagccg
     1021 aaaaaaagga aattgagctg gttgatagcg gcaaagcctt agaggatgta gatgaacttc
     1081 tagagaagtt tgacaaagaa tcttctaagc taagaaagat gggaaagcca tattctctta
     1141 ttgtggcagt tattgcgatt tccatgtcct tatttcattt atatactggg ggctttggta
     1201 ctcttttagc agtaaagcag cgctcccttc acctgatttt tactatggtg ctaggtttta
     1261 tgctctatcc tgcctttgga aaaagcgaga aatcaaggcc tagcataatg gattttatcc
     1321 ttattatcct ggccatagtt gtttttggct atttgtttat gaatgtagaa gccattgttt
     1381 taaagggggg gaaattaaca aaagttgatt tctactttgg ccttattgct attttactta
     1441 cattagaact aacacgcagg gttgtaggcc ctgagcttcc ccttgtagcc ataatctttt
     1501 tgctctatgc ctactttggt cgctcggttc caggcatttt gtcccataga ggcttttctc
     1561 ttaatcggat tattagccat atgtatttta ccaccgaggg aattttggga attcctcttg
     1621 gagtttccgc aacctttgtt tttatgttta tactctttgg ttcttttcta gataagacag
     1681 gagtgggaaa gttttttatt gatattgcct atgccctaac aggaggaatg aaaagcggac
     1741 ctgctatgac cagcgttgtt tccagtggac tgatgggcag tatatcgggc tcatcggtag
     1801 ccaatacggt aacaacaggg gcctttacca ttcccctgat gaagcgcact ggctatgaac
     1861 cacattttgc tggagctgtg gaggcaactg cttctacagg cgggcagatt atgccaccag
     1921 ttatgggggc tgctgctttt attatggcag attttactgg tattccttat attcatatcg
     1981 ttttagctgc cttgattcct gccctactct attatgtagc tgtaggagtt atggttcact
     2041 tagaggcttc aaaactgggc cttaaaggaa tccccaaaga tcaactcccc agcgtgccca
     2101 atattttgct ggaaagtggc tatctaatgg tcccacttat ggttgttgtc tatatgctgg
     2161 taaaacagta tacaccgact cgctcggcct ttgtcgcgat tttggttgct atccttgttg
     2221 cttatatagc tgccctgatt aaaaaagaca agtcctttaa ctataagagc gttttatctg
     2281 ctttagaaaa tggggccaag ggtgctctag gcgtggcctg tgcttgtgcc tgtgctggaa
     2341 tgattgttgg ggttgtaacc ctaacaggct ttggtcttcg tgttgctgag attattgtaa
     2401 cagtagctcg gggaaacctt atggcgactc tttttctaac catgattgct tctttgattt
     2461 taggcatggg tctacctaca accgcaaaat acatagttct tgccaccatg gctgtcccag
     2521 cccttacaaa actcggagta aacctaatgg cagctcattt atttattctt tactttggtg
     2581 ttgtggctga tgttacccct cctgtggccc tagcggccta tgctggagca ggaattgctg
     2641 gatcaaattc tatgaaaaca ggagtccagg cctttaaact ggcccttgca ggatttacgg
     2701 ttccctttat ttttgccatt gattcccatt taatcctggt tgagagtgtg gttggtacca
     2761 cggtaacttt tattcccttt taccaggccc ttccaaccat tgccacggcc attcttggaa
     2821 ttttttgttt ggcaacggct gtggcaggct atatgctaac gacaagtaag ttttatgaaa
     2881 ggattattct ttttgtttca gctctaatgc tcttaaaacc tggctatctt accgatggcc
     2941 tgggtcttgg agtactaata atcatctacc tagttcaaaa aacccgccaa aaaagagaaa
     3001 ctctagccgg cttataggta agaaaaaaac cggacacaac tgggtttttt attcgtaaaa
     3061 aaaaggggta tattagattt aatagaaaga ggaggtagat gatgagtaaa atttatacca
     3121 aaggtggcga taaaggcgga acatcgctct atgatcaggt gcgtgtttct aaagatgatg
     3181 ttcgtgtaga aagctatggt accgtggatg aactaggagc ggtcctgggc ctggcgaaaa
     3241 actttgtaga agatgaacag atgcgagaga ccataagcgc gattcagcac aagctcttta
     3301 cagtggcctc taatcttgca acccaagatc agcaaaaagt agctgctaaa attcatgaag
     3361 aagatatttt gcaacttgaa aagattgtag ataagtatat ggatgcagtc caaagaaaag
     3421 actgctttat tgtaaatggt tcaggaagtc gttcggcttt tttacatgtg gcccgaacgg
     3481 tttgtcgaag agccgagagg agggctgtta gtttatctgg acttgctgag gtggaccctc
     3541 ttgtaattaa atatctcaat cgtctttccg atgccatcta tgctatggca agattcaatg
     3601 aagataagga agagtgtgtt gactatagca aataaaacaa attagaaaaa ggcagagcca
     3661 tcaaggaaaa tgctttgatg gcttttgtct ttagaaggct cgtaaagcat cttcatcagt
     3721 tgctgtatag acctttttat tggtagcttg gggaagggtg ataaagacaa agatcataag
     3781 aagtaaaaag aaggagagga taaccatgct ttttctgggt ccgattaaat ctcctaacca
     3841 gccaccaaaa aactggaaaa tcaccataga aaaactagag gccatttgag caaaggcgag
     3901 gaccttggcg cggagccttg gtggaagata agcttggaca ctggtctcgc gaagggtagc
     3961 cgaagtcacc cccaggcttc cggcgacaaa tcggttgatc agcataaggg gatagcaaag
     4021 aaataaaagc atgatatcaa ggatatcgta gcagtaatag acaaactggg taatgggaaa
     4081 gcgtttttta ggcgggatgt ttaccttata ctggactagg ccacccaggg cccttcctag
     4141 agtttcagct gtagtcagaa aacctagttt tgtgacacct aagatcagtg atttttgaaa
     4201 aaagacctgg atatagagtt gacccgccat agcaactcca ctagaaaggg acatatagct
     4261 atagatgttt aaaagacctt tttctttttt taagtaagtt actgcttctt tcatatcttt
     4321 gaaaaaagca gtaaaatcca tatcatgtcc tggagtcttt ccctggttgt cgataaaggt
     4381 ttctgctgtg gctgctaggg tgcaaaggac ccctaccagg ataaaaagat aatgcatggg
     4441 gatggactta tacataaaag ctgagatagg ggccataacc atcattattg taggataaag
     4501 ggtggagcta acggcatagc ctttttgcat atgacccttg ggaattagca gggggtacca
     4561 ggcttgatag gcaatcatat agacagcact gtttgagcca actaaaaagg ccagggccaa
     4621 ataatggcta tagacaaaac ctccccttgt aagataagca aaaaagaaaa aggtaagagc
     4681 catcaaataa tctagggcca caataatgta tttttttggc gcccggtcaa caaagggaga
     4741 aaccaaaagg ggcaggatta gattgggtag catggcaaga atcattaggt aacttgactt
     4801 taaaagagaa cccgtattgg caaaagtcat tagggataag ggaagttgca tggcttggcc
     4861 tgccatggcc gatataactg taccgctaaa aataagttta aaatctcttg tccaaagtgt
     4921 gtccataaag agcctttctg ggcataaaat cgtaatatta aaggatgcct acaatgctat
     4981 at
//
LOCUS       DASRNA010000047         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_161887,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000047 DASRNA010000000
VERSION     DASRNA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161887"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFD08_01390"
     CDS             <1..394
                     /locus_tag="VFD08_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZK09699.1"
                     /translation="LISALTYAFYVVYLAKSGLRELGSMALLFHICFFGSLQTGLVTT
                     LFKSWSPMNAFGVMGSVVFALVVSFGAMSFQKGTFIIGPQKASILSTLEPLTSILVGL
                     LLLGDRITLISGSGLVLILLSSFVLSIE"
     gene            531..2018
                     /locus_tag="VFD08_01395"
     CDS             531..2018
                     /locus_tag="VFD08_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK09700.1"
                     /translation="MKRKMCLITLVVFALLVSSCSGPEQIETSDTVLRMAKSVDPDGL
                     DPQRSVAESTFEVTSVLYDTLMEVEEDGSLAPGIAKTYSLSEDSLSLSFVIKEGLRFH
                     NDRVVDAQAVKDSFLRLQEEASPRGKDYANFIQINVLSEYEVEFVLQEPDSQALNLFA
                     YPWAAIVEASADNLRAKPVGSGAYKLVEWIPQQHIYLEAFDGSYRDAKIPRVEVLMIP
                     DTSAQITSIEKGELDFIEIYAENIESIDTDKVNLLRFPRNGIQLMAMNSANPYLAQKE
                     VREAIAHGINIPDIKEAVWKGVGISIGSHYPPGVKGYLDLTGKYPYDPARSEEILKDL
                     DLYDKITLRMMLPKTYPEYVAVGQIIANQLEQVGFEVDQQIIEWASWLEDVYNNRDYD
                     LTVVGHTGRLEPYALLAKYLSDGSENYFNYKNPELDELLHQALDEQEADKRTQLYQEM
                     QEILAEEIPAIYLQTPEQIFVTSKNLQGFRQFPITIMNLRDLYFE"
     gene            2027..2965
                     /locus_tag="VFD08_01400"
     CDS             2027..2965
                     /locus_tag="VFD08_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK09701.1"
                     /translation="MLKYALRRLASALIILLVVSMITFVVLRMIPGDPAQLMLGTDAS
                     EELIETLRQDMGLNEPLYKQYIFWIVGVVQGDLGQSYYFGENVARLISQRLPVTLWLA
                     FFSVLTAFLLAIVLGVFSATKRGSRLDVFNRILIALGGSVPGFWLGMMLISLFSVRFE
                     IFPISGLGQGFVRGLLLPMIVLSLGELSTMMRIVRSSILTALQDDAMWSVRVRGLPPR
                     IRLFKYALRSAMIQPITIAALEVGKLFGGTIIVETLFALPGIGRLLLVAVEQRDLILL
                     QGVVLYIVSVIVVISFFTDLLYAYLNRGIPAEKEVR"
     gene            2965..>3623
                     /locus_tag="VFD08_01405"
     CDS             2965..>3623
                     /locus_tag="VFD08_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK09702.1"
                     /translation="MKRDFALYLGLFLVGVVLVLFILSFTPLADQAQVMDLKNTFAGP
                     SREHIFGTDQFGRDIFSRIILSTRYVFLVSLGAVSLGAFIGIILGSIAGIGPPFFSTL
                     ILRIMDGLLAFPGILLALMLVFVLGKGIKNSLIAIAVFMIPVFCKMTYSMILEKRNIL
                     YIKAAKSYGMSNFNIVLQHMLPQMMPKLLTQFTSSMSRAILTEGSLSFLGLGIQPPAA
                     SL"
BASE COUNT          875 a          784 c          893 g         1071 t
ORIGIN      
        1 ccttatttct gctcttacct atgcttttta tgtggtttat ctagccaaaa gcggcctaag
       61 ggagctgggt tctatggccc ttctttttca tatttgtttt tttggctccc tgcaaacggg
      121 ccttgttacc actcttttta agtcctggag cccaatgaac gcttttggag ttatgggcag
      181 tgtggttttt gccctggtgg taagttttgg ggccatgagt tttcaaaagg ggacttttat
      241 tatagggccc caaaaggcct ctattttaag taccctagaa cccctaacca gtattcttgt
      301 gggccttctt cttctaggag ataggattac ccttatttca ggcagtggcc tggtcctaat
      361 tcttttgtct tcttttgttc ttagtataga gtagagagat ttacctaaga aatgattagt
      421 gagataagat tatgtaggaa gggtagttaa gaagaaaggc ccgctttttt tctagggccc
      481 tcttgtgata taataggcct gaaaatcttt ttttaaaaaa ggagtataga atgaaaagga
      541 aaatgtgtct tattacttta gtggtatttg ctctgctagt gagcagctgc tctggacctg
      601 agcaaataga aactagtgac acagtgcttc gcatggcaaa gtctgtggac cctgatggac
      661 tagaccccca aagaagcgtc gctgagagca cctttgaagt cacctcggta ctttatgata
      721 ccctgatgga agtagaagaa gatgggtctc ttgccccggg catagccaag acttacagcc
      781 tttcagaaga tagtcttagc ctgagctttg taataaaaga aggcctgcgc tttcataatg
      841 atagggtagt ggacgcccag gcggtaaagg actccttcct gcgactgcaa gaagaagcca
      901 gcccccgggg caaggactat gcgaatttta tccagatcaa tgttctttct gagtatgaag
      961 tagagtttgt cctccaggag cccgatagcc aggccctaaa tctatttgcc tacccttggg
     1021 cggccattgt agaggcctcg gctgataatc taagggccaa accggtagga agcggggcct
     1081 ataagctggt ggagtggatc ccccagcaac atatttattt ggaggccttt gatggaagct
     1141 accgggacgc taagattccc cgggtagagg ttcttatgat tcctgataca tcggcccaga
     1201 ttacctccat tgaaaaggga gaactggatt ttattgagat ttatgcagaa aatattgaaa
     1261 gtatagacac agacaaggta aacctgctgc gctttcctag aaatggcatc cagcttatgg
     1321 ccatgaactc ggctaaccct tatctagccc aaaaagaggt aagagaggcc atcgcccatg
     1381 ggatcaacat tcctgatatc aaggaggccg tctggaaggg agtgggaatt tctattggaa
     1441 gccactatcc tccgggagta aagggctatc ttgatctgac ggggaaatac ccctatgatc
     1501 cagcaaggag tgaagagatc cttaaggacc ttgatcttta cgataaaatc accctgcgca
     1561 tgatgctacc caagacctat cctgagtatg tagcggtagg gcagattatt gccaaccagc
     1621 tagagcaggt gggttttgaa gtagaccagc agattataga gtgggcctcc tggctagaag
     1681 atgtctataa taatagggac tacgacctta ccgtggtagg ccatacgggc aggcttgagc
     1741 cctacgccct gttggcaaag tatctatccg atggaagtga gaactacttt aattataaga
     1801 atccagagct tgatgagctc ctccaccaag ctcttgatga gcaggaggcg gataaacgca
     1861 cccagctcta tcaagagatg caagagatct tagcagaaga gattcctgct atctatttac
     1921 aaacgccaga acaaatcttt gttaccagta aaaacttaca aggctttcga cagtttccca
     1981 ttaccattat gaacttaagg gacctgtatt tcgaatagga taaataatgc tcaaatacgc
     2041 tttaaggcgc ctggcaagtg ctctaataat acttcttgtt gtcagtatga ttacctttgt
     2101 ggtgctgcgc atgatccctg gagatcctgc gcagcttatg ctgggtacag atgccagtga
     2161 agaattaata gagaccttac gccaagatat ggggctaaat gagccccttt ataagcagta
     2221 tatcttctgg atagtaggag ttgttcaggg ggatcttgga caaagctact actttggaga
     2281 aaatgtggcc cgtcttattt ctcagcgcct tcctgttact ttatggctgg cttttttttc
     2341 tgtcctaaca gccttcctac tggccattgt cctgggagtg ttttcagcga ccaaaagagg
     2401 gtccaggctg gacgttttta accggatcct tattgcccta ggagggagtg tgcctggttt
     2461 ttggctgggc atgatgctga tttctctttt tagtgtgcgc tttgaaatct ttcccatctc
     2521 tggcctaggc cagggttttg ttcggggcct gcttcttccc atgattgtcc taagtctggg
     2581 ggaattaagt accatgatgc ggattgtgcg cagcagcatc ctaacggccc tgcaggatga
     2641 tgccatgtgg agtgtgcggg tgagggggct gcccccacga attcgtcttt ttaaatacgc
     2701 ccttcgctcg gccatgatcc agcccattac cattgcggcc ctagaggtag gaaagctttt
     2761 tggaggaacc attattgtgg agactctttt tgccctgcct ggtataggaa gactcctgct
     2821 ggttgcggta gagcaaaggg atttaatcct tctgcaaggg gtggttcttt atattgtttc
     2881 ggtgattgtt gtgatttcct tttttaccga tcttctttat gcctatctta atcggggcat
     2941 cccggcagaa aaagaggtgc gctaatgaaa agagattttg ccctttattt gggacttttt
     3001 cttgtgggtg ttgttctggt cttatttatt ctttctttta cccccctggc cgaccaggcc
     3061 caggttatgg acttaaaaaa cacctttgct gggccctcta gggaacatat ttttgggaca
     3121 gaccagtttg gtagggatat tttttcccgc attatcctat ctaccaggta tgtgttttta
     3181 gtcagcctgg gagcggtcag cctgggggct tttattggaa ttatcctggg cagtattgcg
     3241 gggattggtc cgcctttttt tagcaccctc atccttagga ttatggatgg gcttcttgcc
     3301 tttcctggga tccttttagc cctgatgctg gtttttgttc tgggcaaggg aattaaaaac
     3361 tccctgattg ccattgccgt ttttatgatt ccggtctttt gtaaaatgac ctattcgatg
     3421 attttggaaa aaagaaatat cctctatatc aaggcggcca aaagctatgg gatgagtaac
     3481 tttaacattg tcctccagca tatgctgcct caaatgatgc ccaagctcct tacccagttt
     3541 acttctagca tgtcccgggc catcctgacc gaaggaagcc taagctttct agggctggga
     3601 atccagcctc ctgcggccag tct
//
LOCUS       DASRNA010000048         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_235384,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000048 DASRNA010000000
VERSION     DASRNA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235384"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFD08_01410"
     CDS             <1..927
                     /locus_tag="VFD08_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HZK09703.1"
                     /translation="YQGIFPYNLMKDFLGLDDSYFTLDGIEHYGSVNFMKAGIQYADA
                     VTTVSRTYAKEISYPYFGEGLHGLLQHKQDKLTGIVNGIDYDEFNPSSDKELWVNFNS
                     SFPQRRLKNKKALQKALGFHVDEKIPMIAMVTRLVDNKGLDLLSFIFDELMEENIQFV
                     LLGSGDKAIENQFIHFQDKYPQKASAHIYFGESFARKIYGSSDIFLMPSRFEPCGLGQ
                     LMAMRYGSIPVVRKTGGLADTVIPYNRYTGEGTGFAFENYNAHELLFAIKDAIYHYNA
                     PESWWNIVKQAMEKDFSWKESASLYEELYEKL"
     gene            914..>2179
                     /locus_tag="VFD08_01415"
     CDS             914..>2179
                     /locus_tag="VFD08_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HZK09704.1"
                     /translation="MRNSKSLGAVKEGENWLFRVFSTREKLSLALFPDPEKDKHRIYS
                     MEKKGDLFELSLAGDYEGWGYGYIYQDQLIVDPYAKYVSHKAKRGILLVDKKSDPPGF
                     SEHKPPRLSYKDAVIYEQHLRDFSMDPRVKFKNPGKFLALCERPKSGGNLIGTDYMAA
                     LGISHVHLLPITPIMSLEAEDGYNWGYDPEYYFAAHRGYLVAVDEPDIALSEIKKSIM
                     HLHGMGLGVVLDVVYNHTYRTKDSSFEALAPGYYYRMKGEEFLDGSGCGNELDTSKPY
                     VQNLIIDSLLYWVEEFKVDGFRFDLWGLMDKNLAEKIVTSLRQVHPQILIYGEPWGSS
                     QQAGQYVHYGSQKGEDYALFSDRYRDGLRGRNDDTSKGYIQNNILSRNMVLTGIVGDI
                     DFSPGLFGYTKEPWETVNYMSCHDNLILYD"
BASE COUNT          651 a          396 c          498 g          634 t
ORIGIN      
        1 taccagggga tcttccccta taaccttatg aaagattttc ttggtctaga tgatagctac
       61 ttcaccctgg atgggattga acattatggt tctgttaact ttatgaaagc aggcatccag
      121 tatgctgatg ctgtaacaac ggtttctaga acctatgcaa aggagattag ctacccttat
      181 tttggagagg gtcttcatgg acttcttcaa cacaaacaag ataaattaac agggattgta
      241 aatggaatcg attatgatga gttcaatcct tctagtgata aagagttatg ggtaaatttt
      301 aatagtagtt ttccccagcg ccgcttgaaa aacaaaaagg ccctgcaaaa ggccctaggc
      361 tttcatgtgg atgaaaaaat acccatgata gctatggtta ccaggttggt ggacaataag
      421 ggactggatc ttttaagctt tatttttgat gaattaatgg aagaaaacat ccaatttgtt
      481 cttctgggta gtggagacaa ggccattgaa aatcaattta tacattttca ggataaatac
      541 ccacaaaaag ccagtgctca tatttatttt ggagaaagtt ttgcccggaa aatctatggt
      601 tctagcgata tctttcttat gccctcgcgc tttgagccct gtggcttggg tcaactaatg
      661 gccatgcgtt atgggtccat tcctgttgta agaaaaacag gaggcctagc tgatacagta
      721 attccttata atcgttatac tggtgagggc acaggctttg cttttgaaaa ctataatgcc
      781 catgaattgc tttttgctat taaagatgct atttatcatt acaatgctcc agaaagctgg
      841 tggaatattg ttaagcaggc catggagaaa gattttagct ggaaagaatc agcatcccta
      901 tatgaggaat tatatgagaa actctaagtc gttgggagca gtaaaggaag gtgaaaactg
      961 gctatttcga gtgttttcta ctcgtgaaaa acttagttta gccctttttc ctgatcctga
     1021 aaaagacaaa caccgcatct atagcatgga aaaaaaaggg gatctatttg aactttcttt
     1081 agcaggagac tatgaaggtt ggggctatgg ctatatttac caggatcaac tcatagttga
     1141 tccctacgct aaatatgtat cccataaggc caaaagggga atcttgcttg tagataaaaa
     1201 aagcgatccc ccgggctttt ctgagcataa gcctcctagg cttagttata aagatgcggt
     1261 aatttatgaa caacatctga gggatttttc catggacccc agggttaagt ttaaaaaccc
     1321 tggcaaattt ttagcccttt gtgagcgacc aaaaagtggg ggcaatctta tagggacaga
     1381 ttatatggct gctctaggca ttagccatgt tcatctgctt ccaattacgc ctattatgag
     1441 cctagaagca gaagatggct ataactgggg ctatgacccg gagtattatt ttgctgccca
     1501 caggggctat ctggtggctg tggatgaacc ggacattgct ctttctgaga taaaaaaatc
     1561 cattatgcac ctgcatggca tgggccttgg tgtggtgcta gatgttgtct acaaccatac
     1621 atatagaaca aaagacagca gctttgaagc gcttgctcca gggtattact accgaatgaa
     1681 gggggaggag tttctagacg gatcaggatg tggcaatgag cttgacactt caaagcccta
     1741 tgtgcaaaac ttgattatag atagcctcct ttactgggta gaggagttta aggttgatgg
     1801 ttttcgcttt gatctctggg ggcttatgga caaaaaccta gcagaaaaaa ttgttacaag
     1861 tcttaggcag gtccatcccc aaatcttgat ttatggagaa ccttggggtt ctagccaaca
     1921 agcaggccaa tatgtccact atggctcgca aaaaggagag gactatgctc tttttagcga
     1981 tagatacaga gatggcttgc ggggaagaaa tgatgataca agtaagggct atatccagaa
     2041 caatatttta tccaggaaca tggtattaac aggcattgta ggagacattg acttttcccc
     2101 cgggctattt ggttatacca aagaaccttg ggaaacggtt aattatatga gttgccatga
     2161 caatttaatt ctctatgat
//
LOCUS       DASRNA010000049        11408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_914214,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000049 DASRNA010000000
VERSION     DASRNA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914214"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1210
                     /locus_tag="VFD08_01420"
     CDS             254..1210
                     /locus_tag="VFD08_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZK09705.1"
                     /translation="MELKKHFMKQKMMTKVLISLIPLYIFSVWLYGLRVLSLLALVTL
                     VGLAAEYWVMRLIQGDRARVSEAALVTCALYTLTLPPTTPYWIAGLGILVALILGKGA
                     FGGFGRNVFNPALVGRAFIYVSFPAQMTASWALPYTSFPGGFIRYGVQSLSSATPMIS
                     LETTGALAPIKDLFLGTIGGSIGEGSALLILLAGVYLIYTKTASKTIILSVLLSGLAS
                     SGLFYFAGWTQAPILFNLLSGGFLFAAVFMATDPISAPREQLSKIIYGVIIGFSAIFI
                     RTFSLFTEGIMFAVLIGNSFAPLIERTVKEIKAKKTLKGASK"
     gene            1207..1758
                     /locus_tag="VFD08_01425"
     CDS             1207..1758
                     /locus_tag="VFD08_01425"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZK09706.1"
                     /translation="MKKGYLYTIIFMALVSLVLTSVLALSNAAFLPTIESNTWKAKQI
                     AILDAFGVEAENPEDFFDTYVKEKSFGNLSGYELEGQEPAYAIPFEGAGLWGTIRGYL
                     ALSEDLSTILGITFTEQSETPGLGGRIEEEQFRDQFRQIELKALTYDDELQAITGATQ
                     TSASVLRIINHFHESVLPNLEVN"
     gene            1758..2372
                     /gene="rsxE"
                     /locus_tag="VFD08_01430"
     CDS             1758..2372
                     /gene="rsxE"
                     /locus_tag="VFD08_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HZK09707.1"
                     /translation="MMDLKVIARNNITKENQVLRQILGICSALAVTNLMSNSLVMGLG
                     VAFVTGLSSFTISLLNQWIPQRIRMLIQTFIIAFYVILVNLFLQAYLPDISKQLGPYV
                     GLIITNCIIMGRAEAFAAKNPPIPALIDGFFSGIGYLLVLLVISFIREFLGFGTLFGF
                     DLGLDFTRWTLMVMPPAAFFVISILIWFTSARSIKAEQKQRSRS"
     gene            2369..2962
                     /locus_tag="VFD08_01435"
     CDS             2369..2962
                     /locus_tag="VFD08_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HZK09708.1"
                     /translation="MSPQISPLVIFIASIFTSNMILANFLGMCSYLSISTEFKTASGL
                     GKAVTLVLVITNTANWLIYKYIIVPLNLEYLQYIIFIMVIAAIVQILELFMDRYFPDL
                     HAKLGVFLPLITVNCAILGSTLFMVIRNYSFVQSLLFNFGSGLGWWMAICMLAAIREK
                     MGKAKLPPGLAGPGIAFLITGIMALAFIGFSGIFPVQ"
     gene            2973..4091
                     /locus_tag="VFD08_01440"
     CDS             2973..4091
                     /locus_tag="VFD08_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZK09709.1"
                     /translation="MNFSEIFTTVISITGISVVLAIIISLADMKLNDYGICKININDG
                     NRELEVTGGQSLLNSLAENKIFIPSACGGKATCGLCKLQVMSGAGPLLPTEEPYLTPK
                     EREEHFRLACQVKVKGDMDLLIPEELFNIKEFRTRVDKLENLTHDIKLVRLKLPEGED
                     LNFKPGQFMQFYTKPYGKIKESVFRAYSISSAPSDKGYVEFCIRQVPDGVCTTYVHTA
                     LKEGDEVPLSGPYGDFYYRGNTPKMILVGGGSGLAPLRSLVLDNLEKGVDADMTLYFG
                     AQSLRDLYYTEEFKALEEKHDNFHFIQSLSRPQEDDVWDGNVGRITVALEEDLKNMQG
                     DGSDIEAYLCGSPGFLAAVRKLLTDNGVPASQIFFDEF"
     gene            4101..4568
                     /locus_tag="VFD08_01445"
     CDS             4101..4568
                     /locus_tag="VFD08_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09710.1"
                     /translation="MAKKNPRMKQIQEDMAPGSLTLVGFLGDDTRSLDEIIRQDEALL
                     QKEGIEISDMVKALKRYTFLGVDGLGDPVDSGDYIITVDHFRGWQYCPFKHKKRVSKR
                     NTTLFDKVTKKTLYWSDLSLHLIEEHSFFQGKGSSFRMEPQDLIDVLRLKKTE"
     gene            complement(4636..5829)
                     /locus_tag="VFD08_01450"
     CDS             complement(4636..5829)
                     /locus_tag="VFD08_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09711.1"
                     /translation="MHTLIISAAHQEQDELMKSLEEFRKNLNIDLSFGSLDDLIRQEL
                     SLVFIATGGTEEQFRKILDQLPGKIYLLTHPAHNSLAASLEILAYLQEKGRKGEILHG
                     APEFVGKKLKEIQLLKNTQVKLQGMRLGVFGISDWLIASDVDEDRVKEIAGIDFVSLS
                     IDELLAEVDKGGYEENPWTQKLLDQGYKALEMEKALEIYGALKRLMKKHNLFGFTLRC
                     FDLLGPLQSTACLALSILNAEGYPAGCEGDKKSLLSMVVAQTLLKESVFMANPSSLDP
                     EKEELVIAHCTLPLDYPRKISLNTHFESGIGVALASDFGFEDVTLFKMDSFKHFVAKE
                     GRLIESLHRNDLCRSQMRIHLPGGTDYFLQHPLANHHMVLRGHHSQLIKKFLEYMAAD
                     IKRRD"
     gene            complement(5941..6279)
                     /locus_tag="VFD08_01455"
     CDS             complement(5941..6279)
                     /locus_tag="VFD08_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZK09712.1"
                     /translation="MLKIAVASDKGNVTEHFGHCETFMVFNSQEKEIVEEILLPNPGH
                     KPGFLPRYLHEQGVDVIISGGMGGAAVAIFNENNIEVIIGAKGEAKKAAEAYLKGELS
                     STGSVCHGHD"
     gene            complement(6279..7109)
                     /locus_tag="VFD08_01460"
     CDS             complement(6279..7109)
                     /locus_tag="VFD08_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZK09713.1"
                     /translation="MSQDCDNNCASCSQECEDRKVDFKENLHSKSRVKRVIGVVSGKG
                     GVGKSLVSAMVAVALNRKGYHSAVLDADITGPSIPKSFGIREKALGTDQGIIPIRTKK
                     GIDIMSINLLLENDTDPVVWRGPIVASAVKQFWSDVIWDNVDFMIIDMPPGTGDVPLT
                     VFQSIPVDGIIVVTSPQELVSMIVTKAVNMAKMMDIPVIGLVENMSHFTCPDNGKDYK
                     IFGESHLEETAQEVGLDILARIPIDPELAKACDKGKIEDFEGPWLDKLVEKLEKMEER
                     "
     gene            complement(7093..8010)
                     /locus_tag="VFD08_01465"
     CDS             complement(7093..8010)
                     /locus_tag="VFD08_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK09714.1"
                     /translation="MKQLLILSGKGGTGKTTLASAFIHFSQARAYADCDVDAPNLHLV
                     MGHRNQPEKSDYYGLPTAVIDQSLCTECDLCRAHCRFNAISAEDGYKVDPFACEGCGV
                     CHYLCPAGAITMKNIPAGEMMLYKNKEVFSTARLVMGSGNSGLLVTEVKKAMKNAIET
                     DFAIIDGSPGIGCPVIASLSGVDYVLIVAEPSLSGISDMKRIIKTAHIQGVETAVCIN
                     KFDLCLENTKHIEELCKELGIHFVGKIPFDPMALEMVNSGKTIADVACPSGEAAKDVF
                     DELMGLMKNKNHKNLVKLGLKGEENESRL"
     gene            complement(7988..8833)
                     /locus_tag="VFD08_01470"
     CDS             complement(7988..8833)
                     /locus_tag="VFD08_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK09715.1"
                     /translation="MKIAILSGKGGTGKTLLSVNLAAAAKKAVYLDCDVEEPNGHLFL
                     KPVDNKMKDINVLMPVVDNDLCDGCKKCVDFCAFNALAYAGGELMIFEKICHSCGGCK
                     LVCPQGAISEKERSIGTVEKGVSDDIVIYTGMLDPGEASGTMIIESLLEEVKDDKGLV
                     FIDAPPGSACVVMESIKDADYCVLVAEPSIFGAHNLEMVHELVELFKKPFGVVLNKCT
                     EKENPSEKYCKENGIKILGKIPFSYELGGLTSAGKIAANENEEFGELFLALLKEVQSE
                     ATSHS"
     gene            complement(8842..9201)
                     /locus_tag="VFD08_01475"
     CDS             complement(8842..9201)
                     /locus_tag="VFD08_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZK09716.1"
                     /translation="MKIAIPVDKNNLETNVSGTYGRAAYYYIYDKETKEAVFVENTAA
                     SGSSGVGIKAAQILIDNGVNVLLTPRLGDNAADVLQKAKIAFYKTQGDNLEENLQAFL
                     EGKLEQLSEIHAGLHGN"
     gene            complement(9202..9576)
                     /locus_tag="VFD08_01480"
     CDS             complement(9202..9576)
                     /locus_tag="VFD08_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HZK09717.1"
                     /translation="MYSIKVMEHFMSPRNAHSMPDADAEGHYGDPSCGDSLTMYIKVK
                     DNHIDDISYLVFGCSASIATASMTSVLAKGKSLKEAFNLSEEDIAGALGGLPEEKMHC
                     SNLGAGALRDAIKNYLKNSKEE"
     gene            complement(9576..9947)
                     /locus_tag="VFD08_01485"
     CDS             complement(9576..9947)
                     /locus_tag="VFD08_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HZK09718.1"
                     /translation="MSRPIKERNVCSLPRVNRFGPLNASQRKDSIIMTVDEYETLRLI
                     DFEGLKQEECAAQMKVARTTVQGIYTRARKKMAEALVHGNILMIEGGEYEICDGLFDC
                     GRACMRRRRGGRRHREANQEK"
     gene            complement(10008..10274)
                     /locus_tag="VFD08_01490"
     CDS             complement(10008..10274)
                     /locus_tag="VFD08_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HZK09719.1"
                     /translation="MPRGDGSGPEGAGPLTGRGLGPCSTSARGLGFGRRSFARGFRRG
                     GGRGFGRGFWSGSVDERSDKELLQEQRELLKSQMEAIDDYLEKV"
     gene            complement(10359..>11408)
                     /locus_tag="VFD08_01495"
     CDS             complement(10359..>11408)
                     /locus_tag="VFD08_01495"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HZK09720.1"
                     /translation="IISLLLVLLLFSVSCSKKEEPELAPLVEELEEEIEEVQEEEEEI
                     EEEQQVLVPRSLLSGVEVAGELAELPIVGVMLDNHPNARWQAGLREAEIVYEIRVEGN
                     FTRYLALFQVTDPEVIGPIRSARTPFITRILEYDAIFAHFGASAHGTKSLNSFGVHSV
                     DGMAVGLPVYYRNHSVGKEAPHNAYSSMAEIREYTQSRSFPEKTEFEGYEFYDEPTAP
                     GGEKVEEFTLYIMPGNTTAYSYLSEEGIYHRYKDGALHIDENDGEPLEVTNVIVQFVG
                     NFMVDSEGPTDIGEVGEGKGLFFSQGEQVEITWEKADREAPTVFFDQEGQPLQLNIGQ
                     TFIQVVDENIEINME"
BASE COUNT         3093 a         2598 c         2106 g         3611 t
ORIGIN      
        1 tcattacacc ctccttctgg tattatagca ttatttttca aaatttatcc ctagttatcc
       61 ataaaaacag ccctggggcc ttttgtcttc ctctatatca cttatagcac caatcattag
      121 ctttaagcag attatagccc atcctgagca ataaatcatt caaatagctt agatcctcca
      181 tgactaaaaa cttaaaagtc taattatact ctgctaatct atgctacaat atatcctata
      241 gaagggagga tttatggaat tgaaaaaaca ttttatgaaa caaaaaatga tgaccaaagt
      301 tctcatctct cttattcccc tgtatatttt ttcggtctgg ctctacggcc tccgggtact
      361 atctctttta gccctggtga ccctggttgg cctagctgct gaatactggg taatgaggct
      421 tattcaaggc gatcgcgcta gggtctctga agcggctctt gtcacctgtg cactatatac
      481 acttacgctc ccccccacca ctccttattg gattgctgga ctgggaattt tagttgcctt
      541 aattttaggc aagggggcct ttggcggttt tgggcgaaat gtatttaacc ctgctttagt
      601 tggacgagct tttatctatg tatctttccc cgctcaaatg acagcttctt gggccctgcc
      661 ctatacatct tttcctggtg gttttatccg ctatggtgtc caaagtctaa gcagcgcaac
      721 tcctatgatt tctttagaaa caacgggagc gctagcaccc atcaaggacc tttttcttgg
      781 aacaataggt ggaagtattg gggaaggttc tgctcttttg attcttctag ctggggtcta
      841 tctaatttac actaaaacgg cctcaaaaac cattatactc tcggttcttc tatcgggtct
      901 tgcttcttct ggccttttct actttgctgg ttggacacag gcacccatcc tctttaattt
      961 attaagtggc ggctttttat ttgcagccgt ttttatggct acagacccta tctcggcccc
     1021 aagagaacag ctcagcaaaa tcatatatgg cgtaattatt ggcttttcag caatttttat
     1081 tcgtacattt tcacttttta ccgaaggcat tatgtttgcc gttctaattg gaaattcttt
     1141 tgcccctctt atagagcgta ctgttaaaga aataaaagcc aaaaaaactc ttaaaggagc
     1201 cagcaaatga aaaaaggtta tctctatacg attattttta tggccctggt gtccctggtc
     1261 cttacttcag ttcttgccct aagcaatgct gcttttttgc ccacaattga aagcaacacc
     1321 tggaaggcga aacagattgc catccttgat gcttttggtg ttgaagcaga gaatcctgag
     1381 gacttttttg atacttatgt aaaagaaaag agttttggaa acctcagtgg ttatgagctt
     1441 gagggccaag aaccagccta tgctattccc tttgagggag caggcctatg gggaaccatc
     1501 cgtggttatt tagccctaag tgaggatcta tctaccattc taggaattac ctttaccgaa
     1561 caaagcgaaa ctcctggact aggggggaga attgaagaag agcagtttcg tgatcaattc
     1621 agacagatag aactaaaagc tcttacctat gatgatgagc ttcaagccat tactggagca
     1681 acccagacat cggcatcggt acttcgaatt ataaatcatt ttcacgagag cgttcttcct
     1741 aatctggagg tcaactgatg atggatttga aagttatagc aagaaacaat attactaagg
     1801 aaaatcaggt tctcagacaa atactaggaa tctgttctgc cctagcggta acaaacctta
     1861 tgagcaactc cctggttatg ggtcttggtg tggcctttgt tactgggctt tcaagtttta
     1921 ccatttcact acttaatcag tggatacccc agcgtattcg catgttgatt cagactttta
     1981 ttattgcctt ttatgtgatt ttggtaaatc tatttctcca agcctatctt cctgatattt
     2041 caaagcaact aggtccttat gttggactaa ttataaccaa ctgtattatt atgggaaggg
     2101 ctgaggcctt tgccgcaaaa aacccaccca ttccagctct tatcgatggt ttcttttccg
     2161 ggattggata ccttctggtt cttcttgtca tcagctttat ccgagaattc ctcggctttg
     2221 gaaccttatt tggttttgat cttggactag actttactag atggacccta atggttatgc
     2281 ctcctgctgc attttttgtc atatccattc tcatctggtt tacctcagcc agatcaatta
     2341 aggctgagca aaaacaaagg agtagatcat gagtccacaa atttcacctt tagtaatttt
     2401 tattgcttcg atttttacca gcaatatgat tttggcgaac tttttaggaa tgtgctccta
     2461 tctttctatc tcaactgagt tcaaaactgc ctcaggcctt ggtaaggcag taaccttggt
     2521 gcttgttatc accaatacag ccaactggct tatttataaa tatattattg ttcctcttaa
     2581 tctagagtac ctacaatata ttatctttat tatggttatt gcagccattg tacagatcct
     2641 tgaacttttt atggatagat attttcctga tctacatgct aaattgggag tttttcttcc
     2701 ccttatcaca gtaaactgtg ctattttggg atctacactt tttatggtta ttcgtaacta
     2761 tagctttgtt cagtcacttc tttttaactt tggaagtggc cttggctggt ggatggctat
     2821 ctgtatgtta gcagccattc gcgaaaagat gggtaaagct aaactacctc ctggtcttgc
     2881 aggtccagga attgcctttt tgattaccgg tattatggct cttgccttta ttggtttttc
     2941 tggcattttt ccggtccaat aaggaggctt agatgaattt ttcggaaatc tttactacgg
     3001 taataagcat tacaggaatc tcggtggttc tagctattat catatccctg gcggatatga
     3061 aactcaatga ttacggaatt tgcaaaataa atataaatga cggaaatagg gagcttgaag
     3121 taacaggggg ccaaagcctt ctgaactccc tagcagaaaa taaaattttt attccctcgg
     3181 cctgcggtgg caaggcaacc tgtggtctat gtaagctcca ggttatgtcg ggggctggtc
     3241 cccttcttcc caccgaagaa ccctatttaa cgccaaagga aagagaagaa catttccgcc
     3301 tggcctgcca agtcaaggtc aagggcgata tggacctact tattccagag gaacttttta
     3361 acataaagga attccgcaca agggtggata aattagaaaa tctaacccac gacatcaaac
     3421 ttgttcgcct caagctcccc gagggagaag atttaaactt caaaccgggt cagtttatgc
     3481 agttttacac caaaccctat ggaaaaatta aggaatcggt ttttagagcc tactccatct
     3541 cctctgcccc tagcgacaag ggttatgttg aattttgtat tcggcaagtt cctgatgggg
     3601 tatgcactac ctatgttcac actgctttaa aagagggaga tgaagttcct ctatctggcc
     3661 cctatggaga tttctactac cggggcaata cccctaagat gatcctggtt ggtgggggca
     3721 gtggtcttgc acccctacgt agtcttgttc ttgacaacct tgaaaaggga gtggacgccg
     3781 atatgaccct ctactttggc gcccagagcc taagagacct atactatacc gaggaattta
     3841 aagccctaga agaaaaacat gataatttcc actttatcca atccctttct cgtcctcagg
     3901 aagacgatgt atgggatgga aatgtagggc gcatcactgt agcccttgaa gaagatctta
     3961 aaaatatgca gggagatggc agcgatatcg aagcttactt atgcggaagc cctggctttt
     4021 tagccgcagt aagaaaactt cttactgata atggggtccc agcatcacaa atcttcttcg
     4081 atgagtttta ggaggtagct atggcaaaga aaaacccgcg tatgaaacaa atccaagaag
     4141 atatggcccc tggtagtcta accctagttg gttttttagg tgatgatact agaagccttg
     4201 atgagattat ccggcaagat gaggctcttt tgcaaaaaga aggaattgaa atttctgata
     4261 tggtaaaggc ccttaaaaga tacacctttc ttggagttga tggtttagga gaccctgttg
     4321 atagtggcga ttatatcatt accgtcgatc attttcgcgg ttggcaatat tgtcccttta
     4381 aacataaaaa aagagtcagc aagcgcaata ccaccttatt tgacaaggta actaaaaaga
     4441 ccctttactg gagtgatttg tccttacatc ttattgagga acacagcttt ttccaaggca
     4501 aaggttcgtc ttttagaatg gagccccagg atctaattga tgttttacgc cttaaaaaga
     4561 cagaataagg cgctaagctt tacaaagtaa aaagagacct ttttattaaa tttaataaag
     4621 ggtctctttt tacttctaat ctctcctctt tatatcagct gccatatatt caaggaattt
     4681 ttttattagt tggctatgat ggcctcgtaa aaccatatgg tgattggcaa ggggatgctg
     4741 taggaaataa tctgtgccac caggaagatg tatgcgcatt tggctgcgac ataagtcatt
     4801 tcggtgcagg ctttctatta gtcttccctc tttggctaca aagtgtttaa aactatccat
     4861 tttaaaaagg gtcacatctt caaatccaaa atctgatgca agggccactc ctattcctga
     4921 ctcaaaatgc gtattaaggg atatcttcct aggatagtct aaaggtaggg tgcagtgggc
     4981 aataaccaac tcttcttttt ctggatcaag gctactagga tttgccataa ataccgactc
     5041 ctttaatagg gtctgggcaa ccaccataga tagcaaagat tttttatccc cttcacaacc
     5101 agcaggatat ccttcagcat ttaagattga tagggctaaa caggcggtag attgcagggg
     5161 acctagaagg tcaaaacatc gaagagtaaa accgaataga ttgtgttttt tcattaggcg
     5221 cttgagtgca ccatatattt ccagcgcttt ttccatctcc agggccttat aaccctgatc
     5281 aagaagtttt tgcgtccaag gattttcttc gtatccacct ttatccactt cggctaaaag
     5341 ttcatcaata gaaagggaaa caaagtcaat gccggcgatt tcttttaccc gatcttcatc
     5401 gacatcgctt gcaataagcc aatcggaaat accaaagact cctagtcgca taccttgaag
     5461 ttttacctgg gtgtttttta atagttgaat ctcttttagt tttttcccca cgaattctgg
     5521 tgcaccgtgt aggatttctc cctttcttcc tttttcttgt aaataagcaa gaatttccaa
     5581 agatgctgcc agtgaattat gggcaggatg ggtgagaagg tagatttttc ctggaagttg
     5641 atctaagatt tttcgaaact gctcttctgt tcctccagta gcaataaaga caagactgag
     5701 ttcttggcgg attaaatcat ctaggctacc aaaactcaaa tcaatattta ggttttttct
     5761 aaattcctct aaacttttca ttagctcgtc ttgctcttga tgggctgcgc ttataataag
     5821 tgtatgcata cttcctcctt ttctcttttt acttataccc tatcttttat ttatttctcc
     5881 taaagacgaa aaaagcctat actttctttt gaaaatatag gctttttaca taataattat
     5941 ctaatcatga ccatggcata ccgaaccagt ggagctaagc tcccctttaa ggtaggcctc
     6001 ggctgccttt ttggcttctc ctttagcacc aataattact tcaatgttat tttcattaaa
     6061 gatggctaca gcagcgcctc ccatccctcc agagatgatt acatccactc cttgttcatg
     6121 caaatatctt ggcaggaagc ctggcttatg ccctgggttg ggaagaagga tctcttctac
     6181 aatttctttt tcttggctat taaaaaccat aaaagtctcg cagtggccaa agtgttcagt
     6241 cacatttccc ttatcgcttg caacggctat ttttaacatt atctttcctc cattttttct
     6301 aatttttcca ctagtttatc taaccagggt ccctcaaagt cctcaatctt acccttatca
     6361 caagctttag ccagctcagg atcaatagga atcctggcta aaatatcaag gcctacttcc
     6421 tgggccgttt cttctaaatg actttctcca aatattttgt agtcttttcc attatctgga
     6481 caggtaaaat gggacatatt ttcaactagg cctataacag gaatatccat cattttagcc
     6541 atatttactg ccttggtcac aatcatggaa accagctcct ggggagaggt taccacaatg
     6601 attccatcta ctggaatgga ctgaaaaacg gtaaggggga cgtcccctgt tcctggtggc
     6661 atatcaataa tcataaagtc cacattatcc cagataacat cgctccaaaa ctgtttgaca
     6721 gcgcttgcaa caattggtcc tcgccagaca acagggtcgg tgtcattttc aagaagcagg
     6781 tttattgaca taatatcaat tccctttttt gttctaatgg gaataattcc ttggtctgtt
     6841 cccagagctt tttccctaat accaaaggat tttggaatcg atggacctgt aatatcagca
     6901 tccaagacgg cgctatgata tcccttacga ttaagagcaa cagccaccat ggcagaaaca
     6961 agagattttc ccaccccacc ttttccgcta acgactccga ttactctttt taccctgctc
     7021 ttactatgaa gattttcttt aaaatctacc tttctgtcct cgcattcctg agaacagcta
     7081 gcgcaattgt tatcacagtc ttgactcatt ttcctctcct tttagtccta gtttcactag
     7141 attcttatga ttcttatttt tcattaaacc cataagctca tcaaaaacat ccttggcagc
     7201 ttctcctgat ggacaagcga catcggcaat tgtcttacca ctatttacca tttccagggc
     7261 catgggatca aagggaattt ttcctacaaa atgaattcct aattctttac aaagttcttc
     7321 tatatgtttg gtgttttcta aacacaagtc aaatttattg atacagaccg ctgtctcaac
     7381 tccctggata tgagccgttt tgatgattct tttcatatca ctaatacctg aaagggaagg
     7441 ttcagcaaca ataaggacat aatctactcc actaagagaa gcaataacag ggcagcctat
     7501 gccaggagaa ccgtcaataa tagcaaaatc ggtttcaatg gcatttttca ttgctttttt
     7561 aacttcagta acaagaagtc ctgaatttcc acttcccata acaagtctgg ccgtagaaaa
     7621 aacctccttg tttttatata gcatcatttc ccctgctggg atatttttca tggtaatagc
     7681 ccctgccggg caaagataat gacatacacc acacccttca caggcaaagg ggtcgacctt
     7741 atagccatct tctgctgaaa tcgcattaaa acggcaatgg gcacgacata agtcgcactc
     7801 tgtacatagt gactgatcaa ttaccgctgt aggtaaacca taataatccg atttttctgg
     7861 ttggtttcta tggcccatca ctagatgtaa attaggtgca tctacatcac aatcagcata
     7921 agcccgagct tgggagaaat gtataaaggc actggccagg gtcgttttcc ccgttccacc
     7981 tttaccacta agaatgagaa gttgcttcac tctgaacctc ctttaatagg gccaagaaga
     8041 gctcgccaaa ctcttcgttt tcatttgctg caatttttcc tgctgaagta agtccaccca
     8101 gttcatagga gaagggaatt ttcccaagaa ttttgattcc attttcctta caatacttct
     8161 ccgaaggatt ttctttttct gtacatttat tcaaaaccac tccaaagggc tttttaaaaa
     8221 gttctactag ttcatggacc atctctaaat tatgagctcc aaaaattgat ggctcagcaa
     8281 ccagaacaca ataatcggca tcctttatgc tttccattac aacacaggcg ctccctggag
     8341 gtgcatcgat aaaaacaagc cccttatcat cttttacttc ttctaaaagt gactcaatta
     8401 tcattgttcc tgatgcttct cctggatcga gcatgcctgt ataaataaca atgtcatcag
     8461 aaacgccctt ttccactgtc cctatggatc tttctttttc gcttatagcc ccttgcgggc
     8521 agactagttt gcaaccacca caggagtgac agattttttc aaaaatcata agttcccccc
     8581 ctgcataggc cagggcatta aaagcacaaa aatcaacgca ttttttgcat ccatcgcata
     8641 ggtcattatc aaccacaggc attagaacat taatatcttt catcttatta tccacgggct
     8701 ttaaaaatag atgaccgttt ggctcttcaa catcgcagtc aagatagact gctttttttg
     8761 ctgcagcagc aagatttaca gaaagcaaag tctttcctgt tcctccttta ccactgagaa
     8821 ttgctatttt cataatttag cctaattccc atgaaggccg gcatggattt cgctaagttg
     8881 ctccagcttg ccctctagaa atgcttgcag attttcttct agattatctc cctgtgtttt
     8941 ataaaatgct atcttcgctt tttgaagtac atcagcagca ttgtctccaa gacgtggagt
     9001 aagaaggaca tttaccccat tgtcgataag gatctgtgcg gctttgatgc caactccgct
     9061 gcttcctgat gctgccgtgt tttctacaaa aacagcctct tttgtttctt tatcatagat
     9121 ataatagtaa gctgctctac cataagtgcc acttacattt gtttctagat tattcttatc
     9181 tacaggtatt gctattttca tttattcctc cttagaattt tttaaatagt ttttaattgc
     9241 atcacgtagc gcaccagctc ccaaattaga gcagtgcatt ttttcttctg gaagaccgcc
     9301 tagggccccg gcaatatctt cttcgctaag attaaaagcc tcttttaggc ttttgccttt
     9361 ggccaataca gatgtcatac tggcagtggc tatagaagcg ctacaaccaa atacaagata
     9421 actaatatca tcaatatggt tatcctttac cttaatgtac atggttaacg aatctccaca
     9481 ggaaggatcc ccatagtgtc cctcggcatc agcatcgggc atactatgag catttcttgg
     9541 gctcatgaaa tgttccatta cttttattga atacattatt tctcctggtt tgcctctcta
     9601 tggcgacggc ctccacgcct tcgcctcata caagcccgtc cacagtcgaa aagaccgtca
     9661 caaatttcat attctccgcc ttctatcata agaatatttc catgaactaa ggcttcagcc
     9721 attttttttc tcgcacgtgt gtagatgcct tgaacagtgg tcctagcaac tttcatctga
     9781 gcggcacatt cttcctgctt aagtccctca aagtcgataa gtcgtaaggt ctcgtactca
     9841 tccactgtca taataatact gtcttttctc tgcgaagcat taagaggtcc aaagcgattt
     9901 accctgggca gggaacaaac gttgcgctcc ttaattggcc tagacatctt ttttcctcat
     9961 ttctaatgtg aaagaaccgg agcctcctcc ggttatctca caaatctcta tactttttcc
    10021 aaatagtcgt caatggcttc catttggctt tttaggagtt ctctttgctc ttgcagtaac
    10081 tctttgtcgc ttctttcatc gacgctgccg ctccaaaaac ctcgtccaaa acctcttcca
    10141 ccaccacgtc taaagcctct tgcaaaactt ctgcgtccaa agcctaaacc tctagcagaa
    10201 gttgagcaag gtccaagacc tcgccctgtt agtggtcctg caccttctgg tccagatcca
    10261 tctcctctag gcataaactc acctcctcaa gtttttgaca tatgtcttat actttaagta
    10321 tacacccttt ctgacatatg tcaagaactc tggtggcttt attccatatt tatctctata
    10381 ttttcatcaa ctacttgaat aaaagtttgg cctatattta gctgcaaagg ctggccttct
    10441 tggtcaaaaa acactgtggg ggcttctcga tctgcctttt cccaagtgat ttctacttgt
    10501 tcaccctgag agaagaagag tcctttacct tcacccactt ctcctatatc agtaggtcct
    10561 tcgctatcaa ccataaaatt acctacaaac tgtacaataa catttgtcac ttcaaggggc
    10621 tctccatcat tttcatctat atggagtgcg ccatctttat aacggtggta tatcccctct
    10681 tcagagagat agctgtaagc cgttgtattt cctggcataa tataaagagt aaattcctct
    10741 accttttctc ctccaggagc tgtgggctca tcataaaact catagccttc aaattctgtt
    10801 ttttcaggaa aagacctact ttgcgtgtac tctcgtattt ctgccataga agagtaagca
    10861 ttgtgaggtg cctctttgcc tacactatga tttcgatagt atactggtaa accaacagcc
    10921 atcccatcaa cactgtgaac accaaagcta ttgagacttt ttgtgccatg agcactagcc
    10981 ccaaaatggg caaagatggc atcatactct aggattcgag tgataaaagg tgtacgagct
    11041 gaacgaatcg gtcctatcac ttcagggtct gtaacctgga ataaggccag atatctagtg
    11101 aagtttccct ctacacgaat ttcatagacg atttcagcct cacgaagacc agcctgccaa
    11161 cgggcattgg gatgattgtc cagcataacc cctacaatag gaagctcggc aagctccccg
    11221 gcaacttcaa ctccacttaa taaagagcgg ggaactagta cttgctgctc ttcttcgatt
    11281 tcttcttcct cttcttgcac ttcttcgatt tcttcctcaa gctcttctac aagtggagca
    11341 agctctggct cctctttttt actacaagaa acactaaaga gtaaaagaac aagtagtaaa
    11401 cttataat
//
LOCUS       DASRNA010000050         6377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_613386,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000050 DASRNA010000000
VERSION     DASRNA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613386"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1224
                     /gene="gcvT"
                     /locus_tag="VFD08_01500"
     CDS             124..1224
                     /gene="gcvT"
                     /locus_tag="VFD08_01500"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZK09721.1"
                     /translation="MSLKRTPLYKKHKEAGASMVEFFGWEMPMTYTKLTEEHQAVRNA
                     AGLFDVSHMGEIYVEGKDSAAFMKKMFSNNLDKIKDGAISYGFFLNEKGGIVDDLLVY
                     KRNENSYLCVVNSSNTDKDDAWLRAHMEGFDVSIDNRSMDIAEVAIQGPKAQEILQKV
                     TDFDLDTLPFFHFKEDVLVGGISCLISRTGYTGEDGFEIYFGNDDAEKIWDLLFEAGE
                     GTLKPVGLGCRDTLRFEAALPLYGNELGEKISPLEAGFGIFVDKEGTFIGSDVMKAQR
                     AGELKRKLVGLELVDKGIARQGYPVFDDKNKEIGFITTGYKSPSLNKVIANALIKKDG
                     PSIDDLVYVGIRNKKAKAKVINKRYLQKKTKR"
     gene            1236..1610
                     /gene="gcvH"
                     /locus_tag="VFD08_01505"
     CDS             1236..1610
                     /gene="gcvH"
                     /locus_tag="VFD08_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZK09722.1"
                     /translation="MANLFFTEEHEWVRVEGNKAYLGISDYAQSELGDIVYVELPEMD
                     EEVVVGDDVGVVESVKSASDLFTPVSGTIVEINEDLEDAPELLNEDAMANWILAIELN
                     DKEELNSLMDEEDYKLFLGEDA"
     gene            1610..2953
                     /gene="gcvPA"
                     /locus_tag="VFD08_01510"
     CDS             1610..2953
                     /gene="gcvPA"
                     /locus_tag="VFD08_01510"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZK09723.1"
                     /translation="MHRYIPNNGAPLKEMLEQIGISSMDELFKDIPQELQFKGELNIP
                     GALSEMELLEKTRKLSKKNKSADDLTYFIGGGVYDHFVPLVIDHIISRSEFYTSYTPY
                     QAEASQGTLQAIFEYQSLITQLTGMEVSNASMYDGSNAAIEAALLSIASKRRSNTLLV
                     SEGVHPEVIEVVKSYSHFRDIEVRLIPLDEQGKTDMEKAQEMVDKSVASIMVQNPNFF
                     GVIEDIPSVVAFSKSHKIMSIDYVDPISLGLFEAPGDQGVDVVVADGQSLGNGLNYGG
                     PYLGIMAMRKKDVRKMPGRVAGLSKDLDGNRAFVLTLQAREQHIRRFKATSNICSNQS
                     LNMLIASIYLNTMGKKGLREVAVQSASKAAYLKKALLDTGYFEEVFSGSTFKEFTLRY
                     KGDLDALLEGLEEAGFLGGLHLKRFGREGELLIAVTEKRSKAEMDAYVEAVRRLS"
     gene            2950..4407
                     /gene="gcvPB"
                     /locus_tag="VFD08_01515"
     CDS             2950..4407
                     /gene="gcvPB"
                     /locus_tag="VFD08_01515"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZK09724.1"
                     /translation="MTYNRLIFENSVPGKFAYSLPDLDVPEIDLTSLDTPLREELDLP
                     EVSEGDLVRHYVNISTKNFSIDKGFYPLGSCTMKYNPKINEDIARIPGLSKLHPYQPE
                     STVQGSLELLYELQEYLAEISGMDTMTLQPAAGAHGELVGLMIIKRYHELRGDHHRRK
                     IILPDSAHGTNPATTAMAGFELVEIPSDEDGSVDVDELKAAIDDNLAGLMLTNPNTLG
                     LFEGHIEEVTQIVHDAGGLIYYDGANMNAIMGKARPGDMGFDVMHFNVHKTFSTPHGG
                     GGPGAGPVGVKSFLKEYLPVPLIEKDDDKYFLNYDLPNSVGKVKSFYGNYGIMLRAYS
                     YISTMGGQGLREASEIAVLNANYLMERLKKTYNLPIDKLCKHEFVLSGIQGAPKVSTL
                     DIAKRLLDYGFHPPTVYFPLIIPEAMMIEPTETESKETLDRFSEALETIAKEAIEDPE
                     LLRNAPYTTPVRRVDEVYAAKTLILTYKDMLNEEN"
     gene            4394..5224
                     /locus_tag="VFD08_01520"
     CDS             4394..5224
                     /locus_tag="VFD08_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HZK09725.1"
                     /translation="MKKIDGRLVAEHLQNELKLKVEALNKEGKNPTLAVILVGEDPAS
                     KVYVGGKERVAKKLGLGSLRYDLDASATEAQILELVEKLNQDPDVHGILVQLPLPKEV
                     DENRIINAISKQKDVDCFHPYNLGRLFQGDESGFLPCTPHGVIRLLEFYDIDVEGKDV
                     VIVGRSNIVGKPLFSLMLNRSATVSVVHSKTKDLAKKTKEADILCVAVGRAGFITKDM
                     VKPGAVVIDVGINSIDGKLHGDVARDVGDIASYMTPVPGGVGPMTITMLMDNTIKACR
                     "
     gene            5234..>6377
                     /locus_tag="VFD08_01525"
     CDS             5234..>6377
                     /locus_tag="VFD08_01525"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZK09726.1"
                     /translation="MKTDVQISSEAKMEPIKNLANKMGIKDKDIELYGDYKAKISLDI
                     FEESKKRGKLVLVSAITPTPAGEGKTTTNVGLSMALNKLGVSAVSALREPSLGPCFGI
                     KGGAAGGGYAQVVPMTDINLHFTGDIHAITAANNLLAALIDNHIHQGNILDIDVNRVL
                     WKRALDMNDRSLRNVLVGLGTKADGVPREDGFNITVASEIMAVLCLASSMDDLKVRLE
                     RMVVAFSRTGQPIKCGQLEATGALALLLKDAVKPNLVQTLENTPAIIHGGPFANIAHG
                     CNSVLATELGLLTSDITVTEAGFGADLGAEKFFNIKCRSAELNPDAVVLVATIRALKM
                     HGGKAKNELSSEDLGALKKGVVNLEKHMENLALFGVPFMVAINTFPT"
BASE COUNT         1996 a         1070 c         1449 g         1862 t
ORIGIN      
        1 tatcatagac tagaaagcat ataaactact aagaaaacgt taaagacttt agtaaatcat
       61 tctttagtga taaaagaaga aaaaagggta tgaatttatc agagaagata aaaggaggta
      121 aggatgagtt taaaacgcac acccttatat aagaaacaca aagaggccgg tgcaagtatg
      181 gttgagtttt ttggctggga aatgcctatg acctatacaa agcttactga ggagcaccag
      241 gcagttcgta atgctgcagg tctttttgat gtgtcccata tgggggaaat ttatgtcgaa
      301 ggtaaagata gcgccgcttt tatgaaaaaa atgttttcaa ataatttgga caaaataaaa
      361 gacggtgcta tttcctatgg tttttttctc aatgaaaagg gaggcattgt tgatgatctt
      421 ttagtttata agagaaatga gaactcttat ctatgcgtgg tcaattcctc taatactgat
      481 aaagatgatg cttggcttcg cgctcatatg gagggctttg atgtaagtat agacaatcgc
      541 agtatggata ttgcagaagt ggccattcag ggacccaaag cccaagaaat tttacaaaag
      601 gtgactgact ttgatcttga tacacttcct ttttttcact ttaaagaaga tgtgttagtc
      661 ggggggattt cttgtttaat ctctagaaca ggatacactg gcgaagatgg ttttgaaatt
      721 tactttggca atgatgatgc tgaaaaaata tgggatttac tttttgaagc aggtgaggga
      781 accctaaaac ctgttggcct aggctgtcga gatacccttc gttttgaagc tgccttaccc
      841 ctttatggga atgaattagg tgaaaaaatc tcacccttag aagctggctt tggaattttt
      901 gttgacaaag aaggcacttt tatcggcagt gatgttatga aggcacaacg agcaggtgag
      961 cttaagcgca aacttgtagg tttagagctt gtagacaaag gtattgctcg acaaggatac
     1021 ccagtctttg atgataaaaa taaggaaatt ggtttcatta ctacggggta taaatcccct
     1081 agtctaaata aagttattgc taatgcttta attaaaaaag atggaccctc gatagatgat
     1141 ttagtctatg ttggcatccg caacaaaaag gccaaggcca aagtgattaa caaacgctat
     1201 ttgcaaaaga aaacaaaaag ataatggagg agaaaatggc aaacttattt tttaccgaag
     1261 aacacgaatg ggtacgcgtt gaaggaaata aagcttatct agggatttca gattacgctc
     1321 aatctgaact gggtgatatt gtttatgtag aactgcctga aatggatgag gaagttgttg
     1381 taggagacga tgttggtgta gtagaatccg taaaaagcgc ttctgatctt tttactcctg
     1441 tttctggtac aattgttgaa ataaatgaag atcttgaaga tgctcctgag cttcttaatg
     1501 aagatgctat ggcaaattgg attttagcca ttgaacttaa tgacaaagaa gaattaaatt
     1561 ctctaatgga tgaggaagac tataaattgt ttttaggtga ggacgcataa tgcatcgata
     1621 tataccgaac aatggtgccc ctctaaagga aatgttagag caaatcggca tttcctccat
     1681 ggatgaacta tttaaggaca ttccacagga gctccaattt aaaggagaat taaacattcc
     1741 cggggcacta tcggaaatgg aactcctgga aaaaacaaga aaactctcaa aaaagaataa
     1801 gtcagccgat gatttgactt attttattgg tggtggtgtt tatgatcatt ttgtaccatt
     1861 agtcattgat catattattt caagaagtga gttttatact tcttatacac cttatcaggc
     1921 tgaggcttcc cagggtactc tgcaggccat atttgagtat caaagtctta taacacaact
     1981 aactggaatg gaagttagca atgcatccat gtatgatggg tccaatgctg ccattgaagc
     2041 ggctttatta tctattgctt caaaaagaag aagcaatact cttcttgtta gtgaaggtgt
     2101 acaccctgaa gtaatagaag tggttaaaag ttattcccac tttagagata tagaggttag
     2161 acttattcct ttggatgagc agggaaaaac tgatatggaa aaagcccagg aaatggtgga
     2221 taaaagtgtt gccagtataa tggtgcaaaa tcctaacttc tttggtgtaa ttgaagatat
     2281 tccttcggtc gttgctttct ctaaaagtca taaaattatg agcattgatt atgttgatcc
     2341 aatttcctta gggctttttg aagctccggg ggatcaaggt gtggatgtag ttgtagccga
     2401 tggtcaatcc ctaggaaatg gtttaaatta tggtggtccc tatctgggga ttatggctat
     2461 gcgcaaaaaa gatgtgcgaa aaatgcctgg gcgagttgcg ggtctttcaa aagatcttga
     2521 tggcaatcgg gcctttgttt taaccctaca agcaagagaa cagcatatca gacgctttaa
     2581 agcaacttca aatatttgtt ccaatcaatc actaaatatg ttgattgcat caatctatct
     2641 taataccatg ggtaaaaagg gcctaagaga agtcgctgtt caaagtgcct cgaaggcagc
     2701 ttatttgaaa aaagcccttc tagatactgg gtattttgaa gaggtcttta gtggatccac
     2761 ttttaaagaa tttaccttaa gatataaggg tgatcttgat gcacttcttg aaggtttaga
     2821 agaagctggt tttctaggag gcttacacct taagcgtttt ggtcgagaag gagaacttct
     2881 tattgcagtt acggaaaaac gaagcaaggc cgaaatggat gcctatgtgg aagctgtaag
     2941 gaggctatca tgacttacaa tcgcttaatt tttgaaaact cagtaccagg taagtttgcc
     3001 tattccttgc ctgaccttga tgtgccagaa attgatctta cctctttgga tacaccttta
     3061 agagaagaac ttgatctacc tgaagtaagt gaaggagatc tagttcgaca ctatgtaaat
     3121 atttctacta aaaactttag cattgataag ggtttttatc ctttggggag ctgtaccatg
     3181 aagtacaatc ctaagatcaa tgaggatatt gcgcgaattc ctggactatc taagttacac
     3241 ccctaccaac ctgaatcaac agtacagggg tctttagaat tactatacga acttcaggaa
     3301 tatcttgctg aaatatctgg tatggatacg atgacactcc agcctgcagc aggagctcac
     3361 ggagagctag tgggactgat gattatcaaa agatatcatg agctaagagg agaccatcat
     3421 agaagaaaga ttatccttcc tgactctgcc catggtacca atccggcaac aactgctatg
     3481 gcaggttttg aactggttga aataccatca gatgaagatg gtagcgttga tgttgatgaa
     3541 ttaaaagcag ccattgatga taatctagct ggtttgatgc taacaaatcc caatacactg
     3601 ggcctttttg agggtcatat tgaagaggtt actcaaattg ttcatgatgc tggcggtctt
     3661 atctactatg atggcgctaa tatgaacgct attatgggca aggctcgccc aggagatatg
     3721 ggctttgatg tgatgcattt taatgtacac aagaccttta gtactcctca tggtgggggt
     3781 ggaccaggag ctggacctgt tggagtcaaa tcatttttaa aggaatatct acctgtgcct
     3841 ttaattgaaa aagatgatga caagtatttt cttaactatg atttgcctaa tagtgtaggc
     3901 aaggtcaagt cattttatgg caattatggg atcatgttac gtgcctatag ttatatttca
     3961 accatgggtg gtcaaggtct tcgagaagct tccgagatag cagtgcttaa tgccaattat
     4021 cttatggagc ggttgaagaa aacctacaat ctacccattg ataaattgtg taagcacgaa
     4081 tttgttcttt cgggaattca aggggcacca aaagtgtcta cactggatat agctaaacgc
     4141 cttttggatt atggttttca tccaccaacc gtatactttc ctctgattat tccagaagcc
     4201 atgatgattg agccaacaga aacagagagc aaggagaccc tagatcgctt cagcgaggcc
     4261 ttagaaacaa ttgctaagga agcaatcgaa gatcctgaac tcctacgcaa tgcaccctat
     4321 acaacaccgg tgcgtcgagt tgatgaggtc tatgctgcca agaccttgat attaacctat
     4381 aaggatatgt taaatgaaga aaattgatgg aagattggta gccgagcatt tacaaaatga
     4441 gcttaaatta aaagttgaag cattaaataa agaaggaaaa aaccctactc tagctgttat
     4501 tttagtagga gaagatcctg ctagtaaggt atatgttggt ggaaaagaaa gagtggctaa
     4561 aaagctaggg ctgggtagtt tgcgctatga tctagatgct agtgcgacag aagctcagat
     4621 actagaactt gttgaaaagt tgaatcagga tcccgatgtt catgggattc tggttcaact
     4681 tcccctaccc aaagaagtag atgaaaacag gattatcaac gctatttcta agcaaaagga
     4741 tgtagattgt tttcatccct ataatctggg gagacttttt caaggtgatg aatcaggatt
     4801 tctgccttgt acacctcatg gggtgataag gcttctagaa ttttacgata tagatgtaga
     4861 aggaaaagat gttgttatcg ttggtagaag caatattgtt ggtaaaccat tgtttagtct
     4921 tatgcttaac cgctctgcaa ctgtaagcgt agttcattca aagaccaaag atttagcaaa
     4981 aaaaacaaag gaagctgaca ttctctgtgt tgctgtaggc agggccggtt ttattactaa
     5041 agatatggta aagcccggag cggtagtgat agatgttggt attaattcta ttgatggaaa
     5101 actccatggt gatgtggcaa gagatgtggg ggatattgca tcctatatga ctcctgtgcc
     5161 agggggtgtt ggaccaatga caataaccat gctgatggat aacacaatta aagcttgtag
     5221 atagtgaggt agtatgaaaa ctgatgttca aatttctagt gaagcaaaaa tggagcccat
     5281 taaaaactta gcaaataaaa tgggaatcaa ggataaagat attgagcttt atggtgacta
     5341 caaggcgaaa atttctttag atatatttga agaaagcaaa aagcggggca agcttgtcct
     5401 tgtaagcgct attactccaa caccagcagg cgaaggaaag acaacaacaa atgttggcct
     5461 ttcaatggcc cttaataaac tgggcgtgag tgctgtttct gcccttagag aaccctccct
     5521 gggtccttgt tttggcatta aggggggggc tgctggaggt ggttacgcgc aagttgttcc
     5581 aatgacagat attaaccttc attttacagg agacatccat gcaataacag ctgcaaataa
     5641 tcttttggca gcactcattg acaatcatat tcaccaggga aatatccttg atattgatgt
     5701 aaatcgcgtt ttatggaaac gtgctcttga tatgaatgat cgaagtcttc gcaatgttct
     5761 tgttggactt ggcacaaaag cagatggtgt tcctagggaa gatggtttta atattaccgt
     5821 tgctagcgaa attatggctg tattatgtct tgctagtagt atggatgatt taaaagtacg
     5881 cctagaacgt atggtagttg cctttagtcg tactggtcaa ccgataaaat gtggccaact
     5941 tgaagcaaca ggggccctag cccttcttct taaagatgca gtaaaaccca atctagtaca
     6001 aaccttagaa aacactcctg ctataattca tggaggtcct tttgccaaca tcgcccatgg
     6061 ttgtaattct gttttggcaa cagaacttgg tcttttgaca tcggatatta cggtaacaga
     6121 agctggcttt ggagctgatt taggggcaga aaagtttttc aatatcaaat gtcgctctgc
     6181 agaactaaat cccgatgccg ttgttcttgt agcaaccatt cgtgctctta agatgcatgg
     6241 gggcaaggct aaaaatgaat tatctagtga agacttaggg gccttaaaaa aaggtgttgt
     6301 gaatctagaa aaacacatgg aaaacttagc tctttttggg gtgcctttta tggtggccat
     6361 caataccttc ccaacag
//
LOCUS       DASRNA010000051         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_19372,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000051 DASRNA010000000
VERSION     DASRNA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19372"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1171
                     /locus_tag="VFD08_01530"
     CDS             62..1171
                     /locus_tag="VFD08_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:917..919,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HZK09727.1"
                     /translation="MEETKETIISWLKDEKADLFIAGPAFNAGRYGMACADLCQAVQE
                     QLDIPALTGLYEENPAVEIYKRDLHIVQVGKSAATMRKGASQMAKIADKLLSGQEIGP
                     PEEEGLLPMGKRVNIFRDKAASKRALEMLLHKLNKEDFKTEVEVPVYDAVEAAEPIQD
                     LSRTTIALLTTGGIVPVGNPDHMPAATAKFFKKYSIEGLDRLEEGVFESVHAGYDPVE
                     CNKNPNRVAPLDLLRKKENEGVINKLYPYMYTVTGNSTSVADAIRMGKEIALELKEEG
                     VHGAIMTSTUGTCTRCGATIVKELERKGIPAVHICTVTPISKSVGAPRILGAIAIPHP
                     IGQPSEAEEKEEAIREELINRALDKLTKTSEDVVS"
     gene            complement(1224..>2116)
                     /locus_tag="VFD08_01535"
     CDS             complement(1224..>2116)
                     /locus_tag="VFD08_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK09728.1"
                     /translation="PQLTQGVNMKDHKKAILYILLSALSFTLMQVFVKLSGPEIPLFE
                     KIFMRNLLIFFITFFILKKNKTPIHVPKDLRLPLLLRCVFGYMGVAGFFYATLHLKLG
                     DATAIHQSSPILVVLLSSVFLKEELTKEKLLTVFLGFIGVLLIVKPSFDVSVFPSLVG
                     LSGALGAAFAYIFISYLGKGVKSEIIILAFAAFSSLASLPFLFGNFVVPRGIALFYLV
                     CIGIFGGLGQFFVTNGYKLSKASEISIYSYTGIIFSGLFGMIIFKENPDFMSFIGMAL
                     IILAGYLLFKISQKKTLQKT"
BASE COUNT          743 a          369 c          473 g          531 t
ORIGIN      
        1 gcttaaagaa ggaaaaattg ttaaaacaat tatatgcggc gataattatt atgctgaaaa
       61 tatggaagaa acaaaagaga ctattatttc atggttaaag gatgaaaagg cagatctttt
      121 tatcgctggc cctgccttta acgcaggccg ctatggaatg gcctgtgcag atctatgtca
      181 agcggtacaa gagcaattag acataccagc gcttacgggg ctttatgaag aaaaccccgc
      241 tgttgaaatt tataaaagag atcttcacat agttcaagtg ggcaagtcag cagcaacaat
      301 gcgcaaaggg gcttctcaaa tggccaaaat agcggacaaa ttgctaagtg gacaagaaat
      361 tggaccccca gaagaagaag gccttttgcc catgggaaaa agagtaaata tttttagaga
      421 taaagcagcc tcaaaaagag ctcttgaaat gcttttgcat aagttgaata aagaggactt
      481 taaaacggaa gtagaagttc ctgtctatga tgcagtagaa gcggcagaac caattcaaga
      541 cctttctaga acaacaattg ctttattgac tacagggggt attgttcctg tgggtaaccc
      601 cgatcatatg cccgcagcaa cagctaagtt ctttaaaaaa tactccatag aaggtctaga
      661 tagactggag gagggtgtat ttgaatccgt tcatgcaggt tatgatcctg ttgaatgtaa
      721 taaaaaccct aatcgggttg caccgttaga tctgctaaga aaaaaagaaa atgaaggagt
      781 gataaataag ctatatcctt atatgtatac tgtaacagga aattctactt ctgttgcaga
      841 tgcaatccgt atgggaaaag aaattgcttt ggagttgaaa gaagagggtg tccatggtgc
      901 gattatgact agtacttgag gcacatgtac acgttgcgga gcaacgattg taaaagaact
      961 ggaaagaaag ggaattcctg ctgttcacat ttgtacggtt acaccaattt caaagtctgt
     1021 aggagcacca cgaatccttg gagctatagc aattcctcat cctattggcc agcctagtga
     1081 agcagaagaa aaggaagagg caattagaga agagcttatt aacagggccc ttgacaaact
     1141 aacaaaaaca agcgaagacg ttgtttctta agagcccctt ttttcttaca gcacttgtac
     1201 tgacctttag catataagaa accttatgtt ttttgtaagg tttttttctg gctaatctta
     1261 aaaagtagat atcccgccaa aataattaag gccataccga taaaactcat aaagtcaggg
     1321 ttttctttaa agataatcat tccaaaaaga ccagaaaaga taattcctgt gtaggagtat
     1381 atgctaatct cactagcctt tgataattta tagccattag tgacaaagaa ttggccaaga
     1441 cctccaaaaa tgccgatgca cacaaggtaa aacaaggcta tgcccctagg aacaacaaag
     1501 ttaccaaaaa gaaagggcag ggaggctagg gaggaaaagg cggcaaaggc caggataata
     1561 atttctgatt ttaccccctt gccaagataa ctaataaaga tataggcaaa ggctgcccct
     1621 agagcgccac taaggcccac aagggaggga aagaccgata catcaaagct gggtttgaca
     1681 ataagaagta ctcctataaa gccaagaaaa acagtgagca gcttttcttt agtaagctct
     1741 tcctttagaa aaacactaga gagcaggacg acaagaatag gagagctttg atgaatggca
     1801 gtggcgtcgc ctagctttaa atgtaaggtt gcatagaaaa aacccgctac acccatatag
     1861 ccaaaaacgc agcgcagtaa aagaggaagt cgaaggtcct ttgggacatg tatgggggtc
     1921 ttatttttct taagaataaa aaaagtgata aaaaagatta gtagattacg cataaaaatc
     1981 ttttcaaaga gaggtatttc tgggccagat agctttacaa aaacttgcat tagtgtaaaa
     2041 gatagggcgg ataagagaat atagaggatg gcttttttgt ggtctttcat atttactcct
     2101 tgggtaagtt gtggac
//
LOCUS       DASRNA010000052         9185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_163381,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000052 DASRNA010000000
VERSION     DASRNA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163381"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /gene="dnaG"
                     /locus_tag="VFD08_01540"
     CDS             complement(<1..1635)
                     /gene="dnaG"
                     /locus_tag="VFD08_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZK09729.1"
                     /translation="MGKKYVDGIAKILCENINILDVVGERVPLKKTGKNYKGLCPFHS
                     EKTASFIVSEERQSYRCFGCGASGNSISFIMENENLDFLTAIETLAERYSFDLTPYKN
                     KNTGSVRRDLDTFYELNRLAALRYYSNLKENKRARNYLLDRGLSEKTLVHFGLGYAED
                     GWQNLYDEIGKNLSEQVRKESGLFGEGARGPYDRFRGRIIFPIVDLRRRVIGFGARSL
                     DPEGIPKYLNSTDTPVFNKSFHLYGLNHAKDYRGEEKIIFLVEGYMDVISLYDKGVKN
                     SVASLGTAFTQEQAKLLDRYCSELVILYDGDTAGIEATKRALEVLDDFPMLVKVVTLP
                     SEMDPDDYIQKYGKEGFLEFIDKNALESIEYRIRELENNHDLETIHGKKAYLEEISLL
                     LSRIKKYSEQDLYLRYVARRLGIDPKELRKDLKVVKEEPQKPSARKTFEEAPGILMAL
                     ILQKPWLARKIHSHKWFSLLEDSWQEVILFMLKEEQFDLNKAADIFSLDQLNYMEKCK
                     NIKLSKTDNKYWEDFFRYLLANQLDRRVKKLQLNPDD"
     gene            complement(1637..2647)
                     /locus_tag="VFD08_01545"
     CDS             complement(1637..2647)
                     /locus_tag="VFD08_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZK09730.1"
                     /translation="MKKYSRKDKEAIEDLTLSIEASKSKLSKGRIFPEEEDAYRTCFA
                     RDRDRIIHAKSFRRLKHKTQVFFAPDGDHYRTRLTHTLEVMQIASSVAGIMGLNRDLT
                     EAIALGHDLGHTPFGHSGERVLNSLYPEGFRHNEQSLRVVDCLETRQNKPYSGLNLSY
                     EVRDGILNHTGDNKASSLEGQLIYFSDRIAYLNHDFDDARRAGILKEEDLPRDIRLSL
                     GQTAGKRIDCMVNDLVEWSYKNKTLGFSDEVREAVDALREFMFKYVYLDSHAKKEGGR
                     VKKLIVLLYEYFLEHPEELPEDYRNENNHLGVKDYIAGMTDGYAVNCVQRIFLPKFWQ
                     ER"
     gene            complement(2644..4026)
                     /locus_tag="VFD08_01550"
     CDS             complement(2644..4026)
                     /locus_tag="VFD08_01550"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZK09731.1"
                     /translation="MISMENLVSLSKNRGFVYPGSEIYGGLSNTWDYGPLGVELKKNI
                     KNSWWYYFIQRHRNVVGLDSSILMNPSVWEASGHVSGFSDPLIDCKECKSRFRADHIV
                     EEYFIKNSIHKELIGADDQRLHEIILEDRIPCPSCGKHNFTNIRKFNLMFKTSQGVTE
                     SASTEIYLRPETAQGIFVDFKSIQRSSRLLIPFGVGQIGKSFRNEITPGNFIFRTREF
                     EQMELEFFCKPGTDLEWYDYWKKACWSYLLKMGIKEDNIRMRDHDKDELSHYSIATSD
                     IEYLYPFGWGELWGIADRTDYDLSQHMKISGKDMQYLDPHTNEKYTPYVVEPSVGVER
                     LFLAFLVDAFDEEILEDGSSRNVMRFHPMIAPIKIGVLPLSKKLSPKAEEVFHLLSAH
                     YRVEMDERGSIGRRYRRYDEVGTPFCVTIDFDSLEDNTVTIRHRDTMEQERIDIEELV
                     SYFDERFQYE"
     gene            complement(4076..5161)
                     /locus_tag="VFD08_01555"
     CDS             complement(4076..5161)
                     /locus_tag="VFD08_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HZK09732.1"
                     /translation="MKDITLEMIDEFIHETGFDYDEARAYLLAAEGDVDLAVEAALAK
                     DGMYGKSTIEGLLDHIKKLAKKGTLLRVTVRKDDDVVLNIPVGVGVVGIFFAKYLSAA
                     GLAVALVTGHTVTLEEKNGNVINVQEYLDKAVAKAKEGSGEIGEFVKKGVNESKEIAK
                     GLKDNIEGKIHAAKDTVEDEGEDFFEEAQELYEGVKIDFHEEVEDLKEEAEEIKEELE
                     ESLKKIKEELAVSEEEAKKEIKEEVEKKAKEKAEKEAKEKAEKEAKEKAEKEAKEKAE
                     KEAKEKAEKEAKEKAEKEAKEKAEKEAKEKAEKDAKEKAEKKAKEKAEKKAKEKAEKK
                     AKEKAEKEAKEKAEKKAKKEAEDKKTK"
     gene            complement(5171..5869)
                     /gene="recO"
                     /locus_tag="VFD08_01560"
     CDS             complement(5171..5869)
                     /gene="recO"
                     /locus_tag="VFD08_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZK09733.1"
                     /translation="MRQIELEGVVLQRNKINDRDCYLRVFTRQMGKVDIFARGANHPK
                     NALNIGSSPFCYGLYQVSGRKLQLRSVEVIDSFYDLRNDFSIMLMASFFTKSYLYLFQ
                     EKQVSVEAFELIINSLSVLKKFPALKDKMLAYFLARIIKIMGLMPDLDCVKRSTNGYM
                     LQVEMGEAQEGGEDTEALLLWRDASNMKMSAFLNLSLDSKDLEKAARLMEGFIYAHLG
                     ADLPTLHQEMSEYL"
     gene            complement(5866..6741)
                     /gene="era"
                     /locus_tag="VFD08_01565"
     CDS             complement(5866..6741)
                     /gene="era"
                     /locus_tag="VFD08_01565"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZK09734.1"
                     /translation="MKSGFCAVVGRANVGKSTLVNQLVGEKVSIVSDKSQTTRHAIQG
                     IVTEEKGQIVFVDTPGMHRPKNKLDDFMLERIDDSLIDMDVILFMIDISQGVGPGDVF
                     MAKKLPNNVPKLLLLNKIDLIDQETLFERKKKIDQLGTFDAVMALSALIPETVKGLKD
                     VIFDYLPEGPQYYPEDTFTDLSDEMLISEIIREKTLHYLDQEIPYGIAVVLDEFKQEE
                     KGIYMAATIIVEKKSHKGIVIGKGGRKLKGIGKAARIELSELFSQPVYLELWVKVKSG
                     WRKSSHYVSEYGYKK"
     gene            complement(6738..7124)
                     /locus_tag="VFD08_01570"
     CDS             complement(6738..7124)
                     /locus_tag="VFD08_01570"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZK09735.1"
                     /translation="MLIEELIEKARQAQKMAYVPYSQFPIGAAVLTEDGKVFTGCNIE
                     SASYGATNCAERVAIQKAVSSGYRTFTHLVVVGQDDYTHPCGICRQVIMEFAPKAKII
                     ISGKNDYILYDAEDLLPYAFTGKDLL"
     gene            complement(7114..7584)
                     /gene="ybeY"
                     /locus_tag="VFD08_01575"
     CDS             complement(7114..7584)
                     /gene="ybeY"
                     /locus_tag="VFD08_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZK09736.1"
                     /translation="MKKAMIEIYYKGLEKLSPEEEGALDLALRTSLELEEIQGPGELS
                     VSLVSKEEIQGLNHDYRGINKETDVLSFPQFQTKDELGKQNYKVLGDIVINMDRVRAQ
                     AQEFGHSLLRELVYLSIHSFYHLLGYDHKKNQAKKAMRKKEEEAYGLWEEKNVD"
     gene            complement(7565..8512)
                     /locus_tag="VFD08_01580"
     CDS             complement(7565..8512)
                     /locus_tag="VFD08_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZK09737.1"
                     /translation="MEQKLSINPEVSMQAMCGKFDENVKIIQDQFGVEIFFIGSELLL
                     KGSNESIGLAIKVLRAMENLILAGSDLDLQKLEYLIHMTLSQEQHNLEELDKQVLIAT
                     HKGKLIKPRTKGQDLYCKTIEENTLTFGVGPAGTGKTFLAVTMAARAFRNKEVSKIII
                     TRPAIEAGEKLGFLPGDLQEKVDPYLRPLYDALGDIFGNESFTRLCERGVIEVAPLAY
                     MRGRTLDNSFIILDEAQNTTIEQMKMFLTRFGAESKVVVNGDITQIDLPPGQTSGLKH
                     ATDILKNIEDIAMVYFSFEDVVRHRLVKRILARYEKSHD"
     gene            complement(8780..8968)
                     /gene="rpsU"
                     /locus_tag="VFD08_01585"
     CDS             complement(8780..8968)
                     /gene="rpsU"
                     /locus_tag="VFD08_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZK09738.1"
                     /translation="MTGIKVRPNETIDSALKRFKRETSKAGLLAEIKKRKHYEKPSVK
                     RKKKTDAAKRKRKKNSRD"
BASE COUNT         2534 a         2064 c         1428 g         3159 t
ORIGIN      
        1 atcatcggga tttaactgaa gtttttttac tcttctgtct aattgattag ccagtaggta
       61 gcgaaaaaag tcttcccaat acttgttatc tgttttgctt aattttatat ttttacactt
      121 ttccatatag tttagttggt cgagtgaaaa gatgtctgca gctttgttta aatcaaactg
      181 ttcttctttt aacataaata ggatgacttc ttgccaagaa tcttcaagaa gagaaaacca
      241 tttatgagaa tgtatctttc ttgccaacca aggtttttgc aaaattagtg ccattaaaat
      301 ccccggtgct tcttcaaagg tctttcttgc acttggtttt tggggttctt ctttgacaac
      361 cttaagatcc tttcttagtt ccttaggatc aataccaaga cgtcttgcaa catagcgtag
      421 ataaaggtct tgttcactat actttttaat cctgcttaaa agcaaactaa tttcttctaa
      481 ataggctttt tttccatgaa ttgtttctaa gtcatgattg ttttctaact ctcttattcg
      541 gtattcaatg gactcaaggg catttttgtc aataaattct aaaaaaccct ctttgccata
      601 tttttgaata taatcatctg gatccatttc gcttggaagg gtaaccactt taaccagcat
      661 gggaaaatca tccagcactt caagagcgcg tttcgtagct tcaatgcctg ctgtgtcccc
      721 atcatataaa attacaagtt cggaacaata gcggtctaat aatttggcct gttcctgggt
      781 aaaggcggtt cctaaactag caacagagtt tttaaccccc ttatcataaa gactaatgac
      841 atccatatag ccttcaacaa gaaaaattat tttttcttct ccacgatagt ctttggcatg
      901 attaagtccg tataaatgaa aactcttatt aaaaactggg gtatctgtgg aattaagata
      961 tttaggtatt ccttcagggt ctaaactcct tgcgccaaat ccgattaccc ttcgccgtaa
     1021 atcgacaatg gggaaaataa tacgcccacg aaatcggtcg tagggtcctc ttgcaccttc
     1081 accaaaaagc cctgattctt tacgtacttg ctcgcttagg ttttttccta tttcatcata
     1141 aaggttctgc caaccatcct cagcgtatcc tagcccaaag tgaactaggg ttttctcaga
     1201 gagtccccga tccaagagat agttacgtgc ccttttattt tctttgagat tgctatagta
     1261 ccgtaaagct gccagacgat tcaactcata aaaagtatct aaatcccttc taacagatcc
     1321 ggtattctta tttttatagg gtgtcagatc aaaactatag cgctcagcta gcgtctctat
     1381 ggcagttaga aaatcaaggt tttcattttc cataataaag ctaatgctat tgccactagc
     1441 tccgcaacca aaacagcggt aactttgtct ttcttcactt acaataaaag aagctgtctt
     1501 ctccgaatgg aaaggacaaa gtcccttata atttttacct gtttttttaa gtggtactct
     1561 ttcgcccact acatcaagaa tgttaatatt ctcgcagagg atttttgcaa taccatctac
     1621 atattttttt cccatgctat ctttcctgcc aaaacttagg gagaaaaata cgctggacac
     1681 aattaacagc atagccatca gtcatcccgg caatataatc tttcactccc aaatggttat
     1741 tttcattacg gtaatcttca ggtaattctt caggatgttc caaaaaatat tcataaagaa
     1801 gcactataag ctttttaaca cgaccgccct cttttttggc atgagaatca aggtagacat
     1861 atttaaacat aaactcacgc agtgcatcta ctgcctcacg gacttcatca ctaaagccca
     1921 gagttttatt tttataagac cactcgacaa gatcatttac catgcaatca attcgttttc
     1981 ccgcagtttg acctaggctt aggcgtatat ccctaggtaa atcttcctct ttaaggatcc
     2041 ctgcacgtct tgcatcatca aaatcgtgat tcaaataggc tatcctatca gaaaaataaa
     2101 ttagctggcc ttctaagctt gaagctttat tatcaccagt atgatttaaa attccatccc
     2161 tcacctcata gctaagattc agtcctgaat aaggcttatt ttgccttgtt tccaggcaat
     2221 caacaacccg aaggctttgt tcgttatgac gaaatccctc gggatagaga ctatttaaga
     2281 ctctctcacc actatgccca aagggagtat ggcctaaatc atgtcccagg gcaatggctt
     2341 cggttaaatc acggttaaga cccatgatgc cagccacact tgaagcaatt tgcatcacct
     2401 caagagtatg agtaagtctt gtacgataat gatctccatc aggcgcaaaa aaaacctgtg
     2461 tcttatgctt aagccttcga aaacttttgg catgtataat ccggtcgcga tctctggcaa
     2521 aacaagtacg ataagcatct tcttcttcag gaaaaatccg acctttagat agtttgcttt
     2581 tactagcctc tatactaagg gtcaaatctt ctatcgcttc tttatctttt cggctatatt
     2641 ttttcattca tattgaaaac gttcatcgaa ataactaact agttcttcga tatcaatacg
     2701 ctcttgctcc atagtatctc gatgacggat agtgacggta ttatcctcta ggctatcgaa
     2761 gtctattgtt acacaaaaag gggtgccaac ctcatcatag cggcgatatc tacgcccaat
     2821 acttccacgt tcgtccattt caactcggta atgagcacta agtagatgaa agacctcttc
     2881 cgcctttgga gaaagcttct tagaaagggg taatacacct atttttatcg gagcaatcat
     2941 gggatgaaag cgcataacat ttcttgatga accatcttct agtatttctt catcaaaagc
     3001 atcaactaag aatgctaaaa acaaacgctc aacacctaca cttggttcaa caacataggg
     3061 tgtgtatttt tcatttgtat gaggatcaag atactgcata tccttgccag agattttcat
     3121 atgttggctt aaatcataat ctgttcgatc agcaataccc cacaattcgc cccatccaaa
     3181 gggatagaga tactctatat cgctagtagc aatactataa tgagacagtt catccttatc
     3241 atgatcacgc atacgaatat tatcttcttt gattcccatc tttaacaaat aggaccaaca
     3301 agcttttttc caataatcgt accactctaa atctgttcct ggcttacaaa agaactccaa
     3361 ctccatctgc tcaaattctc ttgttctaaa aataaagttc cctggagtta tctcatttct
     3421 gaaacttttt cctatttgac ctacaccaaa gggaatcagc aaacgagaag aacgctgaat
     3481 agatttgaag tcaacaaaaa ttccttgcgc tgtttctggt cgaaggtata tttcagtact
     3541 tgcactttca gtaactcctt gggaagtttt aaacatcaaa ttaaacttgc gaatatttgt
     3601 gaaattatgt ttaccacaag aaggacatgg aatcctatct tcaagtatta tctcgtgaag
     3661 cctctgatca tctgctccta ttagctcctt atgaatagag ttttttataa aatactcttc
     3721 cacaatatga tcggctctaa atctactttt acactccttg cagtcaataa gtggatcact
     3781 aaaaccacta acatggccac tcgcctccca gacagaggga ttcataagaa ttgatgaatc
     3841 aagtccaaca acattcctat gtctttgaat aaagtaatac caccaactgt tttttatatt
     3901 cttttttaac tctacaccta ggggaccata atcccatgtg ttagatagtc caccataaat
     3961 ttccgagccg ggataaacaa atccgcgatt cttggaaaga gaaacgaggt tttccatgct
     4021 tatcatactt gtgccacgtc ctttcagttt attctagttc atttttttcc ttgttttatt
     4081 tagtcttttt atcttctgct tcttttttgg ccttcttctc agccttttct ttagcttctt
     4141 tttcagcctt ttctttggcc ttcttttcag ccttctcttt ggccttcttt tcagcctttt
     4201 ctttggcctt cttttcagct ttttctttgg cgtctttttc tgccttttct ttggcttcct
     4261 tttccgcctt ttctttagct tctttttccg ccttttcttt ggcttccttt tccgcctttt
     4321 ctttagcttc tttttccgct ttttctttgg cttccttttc cgccttttct ttggcttcct
     4381 tttccgcttt ttctttggct tccttttccg ccttctcttt ggccttcttt tcaacttctt
     4441 cttttatttc ttttttggct tcttcttcag atactgccaa ctcttctttg atctttttaa
     4501 gggattcttc tagttcttcc ttaatttctt ctgcttcttc tttaaggtct tccacttctt
     4561 catggaaatc gattttcact ccttcgtaaa gctcttgcgc ttcttcgaag aaatcttctc
     4621 cctcatcttc tactgtatct tttgctgcgt gaatttttcc ttcaatattg tctttaagcc
     4681 ctttggcgat ttcttttgat tcgttaacac cttttttaac aaactctcct atttccccgc
     4741 taccctcttt tgcctttgct actgccttgt ctaagtattc ttgcacattg atcacatttc
     4801 cgtttttttc ttcgagagtg acagtgtggc cagtcacaag ggcaacagcg aggcctgcag
     4861 ctgatagata ttttgcgaag aatatgccaa ctacaccaac gccaacagga atatttaaaa
     4921 caacgtcatc atccttacgc acagttacgc gaagaagagt tcctttttta gccaactttt
     4981 taatatgatc aagcaggccc tcaatggtag atttaccata cattccatcc ttagccaagg
     5041 ctgcttcaac ggctaaatct acatcccctt cagcggctaa gagataggct cttgcctcat
     5101 cataatcaaa accagtttca tggataaatt catcaatcat ttctaaggta atatctttca
     5161 taatttcctc ctatagatat tctgacattt cttggtgtaa agtgggaagg tcggcaccca
     5221 aatgtgcata aataaaacct tccatcaagc gcgccgcttt ttcaaggtcc ttactatcta
     5281 agcttagatt taaaaaagct gacattttca tatttgatgc gtctcgccat aagagaagag
     5341 cttctgtatc ttctcccccc tcctgggctt cacccatctc tacttgtaac atatacccgt
     5401 ttgtagatct ttttacgcaa tccaaatcag gcatcaagcc cattatttta ataatgcgag
     5461 ctaaaaaata ggcaagcatt ttatctttta gtgcgggaaa cttttttagt acgctgaggc
     5521 tgttaataat taactcaaag gcctctacac ttacttgctt ttcctgaaat aagtaaagat
     5581 agctttttgt aaaaaaagac gccattagca taatagaaaa atcatttctc aaatcataaa
     5641 aactatctat aacctctaca cttcgaagtt gtagttttct accgcttacc tgataaagtc
     5701 cataacaaaa ggggctagag ccaatattaa gggcgttttt aggatgattg gcccctctag
     5761 caaatatatc gactttaccc atttgcctgg taaaaactct tagatagcag tctcgatcat
     5821 tgattttatt tctttgcaga acaacacctt caagttcaat ttgtctcatt ttttatagcc
     5881 atactcactt acatagtgac tggattttcg ccaaccactt ttaaccttaa cccatagttc
     5941 taagtatact ggttgagaaa aaagttcgct taattcaatt cgcgcagcct ttcctattcc
     6001 tttaagcttt cttcctccct tgcctataac aatcccttta tgggactttt tttccacaat
     6061 aatagtagca gccatataaa tgcctttttc ttcttgttta aattcatcca agactacggc
     6121 aattccatag gggatttctt gatcaagata gtgaagtgtt ttttctcgaa taatttcact
     6181 aattagcatc tcatcactca aatccgtaaa ggtgtcttca ggataatact gaggcccttc
     6241 gggcaggtag tcaaaaataa catcttttaa tcccttaaca gtttctggaa taagggcact
     6301 tagagccata acagcatcaa aagttcctaa ttgatcgatt tttttctttc tttcaaaaag
     6361 cgtttcttga tcaataagat cgattttgtt aagtaaaaga agtttgggga cattattagg
     6421 aagctttttt gccataaaaa catctcccgg tcccacacct tgggagatat ctatcataaa
     6481 taaaatcaca tccatatcaa tcaaggaatc atcgatgcgc tctaacataa aatcatctag
     6541 tttgtttttt ggtcgatgca ttcctggagt gtctacaaag acaatctgac ctttttcttc
     6601 tgtaacaatt ccttgaatgg catgtcttgt ggtctgactc ttatcactaa cgatggaaac
     6661 tttttctccc acaagttgat ttactagagt gcttttacct acattggctc ttcctacaac
     6721 agcacaaaat cctgatttca cagcagatcc tttcctgtaa aggcgtaggg aagcaaatct
     6781 tctgcatcat agagaatata atcatttttc cctgaaataa ttatctttgc cttgggggca
     6841 aactccataa tcacctggcg acaaatgcca caaggatggg tgtaatcatc ttgcccaact
     6901 acaacaagat gagtaaaggt gcgataacca gaactcactg ccttttgtat ggcaactcgt
     6961 tcagcacagt ttgttgcacc ataagaagca gactcgatat tacagcctgt gaatactttg
     7021 ccatcttcag ttaaaacagc agctccaata ggaaattgcg aataagggac atatgccatc
     7081 ttttgagctt gtctagcttt ctcaattaat tcttcaatca acattttttt cctcccagag
     7141 cccgtaggct tcttcttctt ttttcctcat ggcttttttt gcttgattct tcttgtgatc
     7201 atatcctagt aagtggtaaa aactatgaat gctcagataa actagttcac gcaagagact
     7261 atgtccaaac tcctgggctt gtgcacgtac cctatccatg ttaataacta tatctcctag
     7321 cactttatag ttttgcttac ctagttcatc ctttgtctga aattgaggaa aactaagaac
     7381 atcggtttct ttatttatac ctcggtagtc atgatttaat ccttggattt cttctttact
     7441 aactaaagaa accgaaagtt cgccaggtcc ttggatttct tccagctcca ggctggtgcg
     7501 taaagctagg tctaaggctc cttcttcttc aggacttagt ttttctaggc ccttataata
     7561 aatctcaatc atggcttttt tcataacgag ctagtatcct cttaacaagg cggtgacgta
     7621 ctacatcctc aaaactaaaa taaaccatgg caatatcctc tatgttttta aggatatccg
     7681 tggcatgctt aagcccacta gtctggcctg ggggtaggtc aatttgggtt atatccccat
     7741 ttacaacgac tttagattca gcaccaaaac gagtcaaaaa catcttcatt tgttcaattg
     7801 ttgtgttttg tgcttcatct aaaataataa agctattatc caaggttcgc cctctcatat
     7861 aggctagcgg ggctacttca atcactccac gctcacataa tctagtaaaa ctttcatttc
     7921 caaaaatatc accaagcgca tcataaagag gccgcagata aggatccacc ttctcctgta
     7981 aatctcctgg aagaaagcct aacttttctc ctgcctcaat agctggacga gtgataataa
     8041 tcttgctaac ttctttatta cgaaaggctc ttgcagccat agtaacagct aaaaaggttt
     8101 tccccgttcc agctggccca accccaaagg ttaaggtgtt ttcttcaatg gtcttacaat
     8161 aaagatcttg tcccttagtt cggggtttta ttagcttacc tttatgggtg gctattaaaa
     8221 cctgcttatc aagttcttcc aagttatgct gttcttgact aagggtcata tggattaaat
     8281 attctagctt ttgcaagtca agatctgatc cagctaaaat aagattctcc atggcacgta
     8341 gaactttaat ggctagacca atggattcat tgcttccttt taataaaagt tcgctgccaa
     8401 taaaaaatat ctccacacca aattggtctt gtatgatttt tacgttttca tcaaactttc
     8461 cacacattgc ttgcatggaa acttcaggat taatcgatag tttttgctcc ataaatacct
     8521 cttatccatt ctagcacaaa ttatcctata cttcttcttt ctggatgatc tttatacaaa
     8581 aataataccc cacatggggt attattaggt tctgatagat ttatctttta caagacaaaa
     8641 gtttttttga tgctttgcct ttgatctata ccactactct tatagtggtt ttataataat
     8701 ctgttgtgtt ttacacaata cagcatttct tgcacagatc ttcaaaatgc tattcatcta
     8761 tcttacgctg gtccctcttt tagtcacggc tgttcttttt tcttttacgc tttgcagcgt
     8821 cagttttctt tttacgcttc acacttggtt tttcatagtg cttgcgtttt ttaatctcag
     8881 ccaatagacc tgcttttgag gtctcacgtt taaaacgctt tagagcgcta tcaatagttt
     8941 cattaggtct tactttaatt cctgtcaatc ttatcccccc cctctttcca agatttgtac
     9001 attgtactgg gagtagcaag tcttattata aaccagaata aatgaaaaat caaatggttt
     9061 tatttgtttt cggcttaaac aaggtcatcc taactttcca aagtaaaggc aacactcact
     9121 tttccaagtg tcccacttac cttaggaaac aaacccctct cctttacctt gagaaactgt
     9181 gtata
//
LOCUS       DASRNA010000053         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_316591,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000053 DASRNA010000000
VERSION     DASRNA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316591"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFD08_01590"
     CDS             <1..243
                     /locus_tag="VFD08_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09739.1"
                     /translation="TRGRAGGFGRGQGRELANAKSGAYQESGRRIRRRDFSCLDGYGG
                     FYNHGFDGKELLKNKKEFLKERIKEIDEILLENEEE"
     gene            272..661
                     /locus_tag="VFD08_01595"
     CDS             272..661
                     /locus_tag="VFD08_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HZK09740.1"
                     /translation="MARPVKLRRVCDLPRRNQFESMGSSSEGLEDVTMTIEEYQTIKL
                     IDLEGLTQEECAESMEIARTTVQRIYMEARKKLAIFLINGSSLKIEGGNYVLCNEIGP
                     GPGCGRCRGRGLGRGRNMNHQGGGRDL"
     gene            658..1017
                     /locus_tag="VFD08_01600"
     CDS             658..1017
                     /locus_tag="VFD08_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZK09741.1"
                     /translation="MIIAIPVEIDSKESLVSEHFGRSNYFLIYNSEEKEYKFVENSAA
                     KSSGGAGIKAAQIVVDENAQVLVSPQLGQNAADVINGAKIKIYKAQVGSIKENLQALE
                     EGKLKLLSEIHGGYHGN"
     gene            1007..1858
                     /locus_tag="VFD08_01605"
     CDS             1007..1858
                     /locus_tag="VFD08_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK09742.1"
                     /translation="MGIKGKKIAVLSGKGGTGKTLVSVNLAALISEANYVDCDIEEPN
                     GYLFLKPKNIKNEKVTRDVPLVNHDKCIGCKECAEFCKFNALAYTSQLIVFENICHSC
                     GGCMLVCPEGALSTKEREIGKIEIGESSGVNNYTGFLKIGEPSGTPIIEKLLERVSTS
                     LLTSIIDAPPGSACTTMDSIKNADYCILVAEPTTFGAHNLEMVYDLLVLFEKPFGVVL
                     NKTLDGENPSKDLCVEKDMKILLEIPFDEELGRLNSKGHILVNESKKYKEMFGDLLHK
                     ITKEVGR"
     gene            1855..>2022
                     /locus_tag="VFD08_01610"
     CDS             1855..>2022
                     /locus_tag="VFD08_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZK09743.1"
                     /translation="MKELLILSGKGGTGKTTIAGAFIKLAQAQAFADCDVDAPNLHLS
                     MELKDEAKVKDF"
BASE COUNT          739 a          274 c          475 g          534 t
ORIGIN      
        1 actagaggaa gagcaggagg ttttggaaga ggtcaaggaa gggaacttgc aaatgcaaaa
       61 tctggagcat atcaagagtc tggtagaaga ataagaagaa gagatttttc atgcctggat
      121 ggctacggtg gattttataa tcatgggttt gatggtaagg aattattgaa aaataaaaaa
      181 gaattcttga aggaaaggat aaaagagatt gatgagattt tattagaaaa tgaagaggag
      241 taatagattt aaaaaataag gaggataagt attggcaaga ccagtaaaac taagaagagt
      301 atgtgattta ccaagaagaa atcaatttga gtcaatggga tcttctagtg aaggcttaga
      361 ggatgtgact atgactatag aagaatatca aacaattaaa ttaattgatt tagaaggctt
      421 aacccaggaa gaatgtgctg aatccatgga aatagcaagg acaacagtac aaaggatata
      481 tatggaagct agaaagaaac ttgcaatctt cctaataaat gggtctagcc taaagattga
      541 aggtggcaac tatgtcctat gcaatgaaat aggaccaggg cctggttgcg gtagatgtag
      601 aggcaggggt ctgggaagag gtagaaatat gaaccatcaa ggaggaggaa gagatttatg
      661 ataatagcta taccagtaga aatagattct aaagaaagtt tagtcagtga acattttgga
      721 agaagtaact actttttaat ttataattca gaggagaaag aatataagtt tgtagaaaat
      781 tcagctgcaa aaagttctgg aggtgctgga atcaaggcag ctcagatagt tgtggatgaa
      841 aatgctcaag tcttagtatc ccctcaacta ggacaaaatg cagcagatgt aataaatggg
      901 gcaaaaataa aaatttacaa ggctcaagtt ggaagcatta aagagaattt acaagccttg
      961 gaagaaggca agttaaagct tcttagtgaa attcatgggg ggtatcatgg gaattaaggg
     1021 caagaaaatt gcagtcttaa gtggtaaggg tggtacaggt aaaaccctag tttcagtaaa
     1081 cttggctgcc cttatttcag aagctaacta tgtagactgc gatattgaag agccaaatgg
     1141 ttatttgttt ttaaaaccaa aaaatataaa aaatgaaaag gttactaggg atgtccccct
     1201 agtgaatcat gataagtgta taggctgtaa agaatgtgct gaattttgta aattcaatgc
     1261 cttggcctac acaagtcagc tgatagtttt tgaaaatatc tgccattcct gtggcggctg
     1321 tatgctggtc tgcccagaag gcgcactttc aactaaggaa agggagattg gaaaaataga
     1381 aataggagaa agtagtggtg ttaataacta tacaggattt ttgaaaatag gagaaccttc
     1441 aggtactccc ataatagaaa aacttttgga acgggtatcc actagcctct taacaagcat
     1501 tatagatgcc ccacctggat cagcctgtac aactatggat agtattaaaa atgctgacta
     1561 ttgtatcttg gttgctgaac caacaacttt cggtgcccac aacttggaaa tggtttatga
     1621 tttattagtc ttatttgaaa aaccttttgg agtagtttta aataagacac tagatggaga
     1681 aaatccatcc aaggacttat gtgtagaaaa agatatgaag atccttttag aaattccctt
     1741 cgatgaagaa ttaggtagat taaattctaa gggccatatc ctagtaaatg aaagcaaaaa
     1801 gtataaagaa atgtttggcg acttgctaca taaaatcaca aaggaggtgg ggagatgaag
     1861 gaactcttga tcctcagtgg caagggtggt actggtaaaa ctaccatagc tggtgccttt
     1921 ataaaactag cccaggctca agcttttgct gactgtgatg tggatgctcc caacctccac
     1981 ttaagtatgg aactcaaaga tgaagccaaa gtaaaagatt tt
//
LOCUS       DASRNA010000054         6458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_19808,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000054 DASRNA010000000
VERSION     DASRNA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19808"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VFD08_01615"
     CDS             <1..1532
                     /locus_tag="VFD08_01615"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09744.1"
                     /translation="PVFKLYDSYKAAGIPLAFLALGFMSYLFMLLIFSFPYILSKIYF
                     SKDIETMLSLPLASEEILASKFLSLTFSSLIYAVFLVIPIMFKFGFSEKMGPVYYIYG
                     FLGLILLSMGIISLLAFVIIFLMRWVVRLPRIKNALQFLGMMLILIFSIGINYFIQSS
                     LSGDPAEMIDQILKSSKGLLDSLLPGIPNLQWLLLAMENSGRLLGLGYFLLLFLATLL
                     LVVVLIKLGAPFMRQGVLANTAIKEKKVRAKGKNKKRSVAVNLFKKELGELLRTPLYA
                     FNTLGGGIILPIVMLIPLIAQGNIGFKELEMAREMIRTFPITSLDLILVSIAIGMVLG
                     IFLGSIGNPVSSSFSREGKQIWIIKSLPILVKDQILGRLLVGMSFQLMLILPTLILIG
                     LVLWPPIGAVLGILLGSLLSGLFISLLGLSVDARRPKLVWDNPQEAMKQNFNLVITML
                     TSWAYLGLVIFILVRIKIDYLENLPLIVGVIVFSQLLIDAGLYIYLNKTMQSSLLRMQ
                     Y"
     gene            1544..2650
                     /locus_tag="VFD08_01620"
     CDS             1544..2650
                     /locus_tag="VFD08_01620"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZK09745.1"
                     /translation="MIVVKGTYNSAKVFTNDLEDTAAKQIKELLEQEFVEGLKIRIMP
                     DVHAGAGCTIGTTMTLEDKVVPNLVGVDIGCGVEVVKLGKQKPDYRSLDELIYRGIPS
                     GFKIRKREHPFLKNVAIDRLICKNKAKLNMDRAKKSLGTLGGGNHFIEIGENEKGEFY
                     LLIHSGSRNIGLQVALYYQKLAQKKQKGKGIPRDLTYLEGKDTENYLHDMALMQNYAE
                     WNRRAMAQIILDGLGYENLGRFSTIHNYIDIEEAILRKGAVSAKKGEIFLLPMNMRDG
                     SFICRGKGNPDWNYSAPHGAGRILSRTQARKKLSLKEFKTSMEGIYTTSAQNRTLDEA
                     PRAYKKMHEILENVEPTLEVLHHLKVRYNYKATN"
     gene            2673..3356
                     /locus_tag="VFD08_01625"
     CDS             2673..3356
                     /locus_tag="VFD08_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZK09746.1"
                     /translation="MFISRVEKTRVKIGHIPALVFEPIDKKAQGHIIHYHGWGSKKEN
                     HSLVASALAAAGFMVLVPDGLYHGERGPFEEEDETLLPDVILSNLREFPLLSSYLKAE
                     KLYLTGHSMGSMSAGVIFHQEKTVDAAIIINGYLSYKDLDLDMEVPKELLEVDPIDFL
                     ANVKDRHLLILHGDADSSVDIKVQREYVKKAQAYYKPGHLIMEEIPSLNHYVTVAMLA
                     RMIDYLSHL"
     gene            complement(3378..4481)
                     /locus_tag="VFD08_01630"
     CDS             complement(3378..4481)
                     /locus_tag="VFD08_01630"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZK09747.1"
                     /translation="MKSNILIAIDSFKGSLSSLEAARAVEKGLRSSLTKSQIRLLPLA
                     DGGEGTLEMLNHFFILDLHQEEIVDLYGLPLKSKIYYNQEIAFLEAAHAAGLQEKTDI
                     YKASSLGFGMELLIARSLSPKKILLGLGGTGVNDGGMGFLHSLGLRFYHDDKELSPSL
                     ESLFYVNRIEGEIASISNLLVAVDVTNPLLGENGASAVYGPQKGLKAKDLPRVDQAMK
                     GYALLLEKHFQKKVKDLPGAGAAGGLGFALLLIGASLKPGFSLLTELCNFNDHLLWAD
                     IVITGEGRFDSQSLQGKGPTELARKAKEKNKAVFGFFGELIAESPLFDGSFSIQQGPI
                     SLEDAMEPKMTQKNLEKTAKSLAGTLKAARKLG"
     gene            complement(4471..5409)
                     /locus_tag="VFD08_01635"
     CDS             complement(4471..5409)
                     /locus_tag="VFD08_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZK09748.1"
                     /translation="MKLVVLDGHALNPGDLSWDELRKFGQLTVYPRTKKEDVKKRIAD
                     ASIVFTNKTPIDEKALEDSKVRFISVLATGYNIVDIQAAKARGIIISNVPSYGTLTVA
                     QFATAMMLELSHQVGRHSKSVHRGDWTRSDDWTYLLSPQIELAGKTLGLFGLGRIGLA
                     FAKIAEALGMEIIYHSRSKKDVPYSFVSLEDLFKESDFLSLHSSLNKQSLGVVNENTL
                     GLMKKTAFIINCSRGPLIVEEDLVNALKKGLLAGAALDVVNLEPMEKDSPLLGVENLI
                     LTPHIAWSTREARKRIMDSSLDNLKAFLEGRPIHEV"
     gene            complement(5390..>6458)
                     /locus_tag="VFD08_01640"
     CDS             complement(5390..>6458)
                     /locus_tag="VFD08_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK09749.1"
                     /translation="STTFLLDAGYSNIGIGLLFASANLASIPLQSFLASAVDNGKIPL
                     KGLMLTQGFAILLVAGLVAWQALAPFYFLLLLFLMSLQPFVNALSVACLHEDIPLDFG
                     VSRGMASLLYSLGSVFLGNLLARRGIFITPYAGMVFIVIYILCVLSFPFPAFYKKEGD
                     IKGNIAKRYPFLYLFLPAVVLLFCGYGMFHSYLIHLIRFVGGDESALGLTLGISAFSE
                     VPTMFSFTWISSRFEEKKLLLFSAFFFALKALLDLFAKNIYMLYINQSLNAVTFGLFL
                     PTAIFYLSHTMEKKDAATGQALLTSAIIAGNVLASFIGGILMDSFGIRVLLQSVSFIQ
                     ILAFILMYSALKGEKNEARRP"
BASE COUNT         1970 a         1271 c         1370 g         1847 t
ORIGIN      
        1 aacctgtatt taaactctac gatagttata aggcagcggg gattcccctg gcatttttag
       61 cccttggctt tatgtcttat ttatttatgc tacttatttt ttcctttccc tatatactta
      121 gtaagattta ctttagtaaa gatattgaga ccatgctgag tctgcctttg gcctcagaag
      181 aaatccttgc tagtaaattc ttgtctctta cctttagttc tttaatctat gcggtttttc
      241 tagtgatacc aattatgttt aaatttggtt tttctgagaa aatgggccca gtctattata
      301 tctatggatt tttggggctg atcttacttt cgatgggaat catcagtctt ttagcctttg
      361 ttattatttt tttaatgcgc tgggttgtac gccttcccag aataaaaaat gcccttcagt
      421 ttttggggat gatgcttatt ttgatctttt ctataggaat caattatttt atccagtcta
      481 gcctaagcgg cgatccggca gaaatgatag atcaaatcct caagagttcc aaggggcttt
      541 tagattctct tctgccagga attcctaacc tccagtggct tcttctggcc atggaaaata
      601 gcggtagact tttaggcttg ggctattttc ttcttctttt tctagcaacc cttcttcttg
      661 tggtggttct tatcaagctg ggagcaccct ttatgagaca gggagttctt gctaatactg
      721 ctatcaaaga aaaaaaagtc agggcaaaag gaaaaaacaa aaaaagatcc gtagcagtaa
      781 atctttttaa aaaagaattg ggagaacttt tacgaactcc tctttacgcc tttaacaccc
      841 taggaggggg aattattctt cctattgtta tgctaatacc tcttattgct caaggaaata
      901 taggtttcaa agaactagaa atggccaggg aaatgataag gacctttccc attacaagtc
      961 tagatcttat cctagttagt attgctattg gaatggtgct tggaattttt cttggttcca
     1021 taggaaatcc cgtatctagt agtttttccc gggagggaaa gcagatttgg attatcaaaa
     1081 gtcttcccat tttggtaaaa gaccagattc ttgggcgcct actagtagga atgagttttc
     1141 agctaatgct tattctacct acgcttattc taataggcct tgtcctttgg ccccccatag
     1201 gagcggtatt agggatttta ctgggatctc ttttaagcgg tctatttatc agtcttcttg
     1261 gtttgagtgt ggatgcaaga aggccaaaac ttgtctggga caatccccag gaggccatga
     1321 agcagaattt taaccttgta atcactatgc ttactagctg ggcctaccta ggactagtta
     1381 tttttattct tgtaagaata aaaatagatt atctagaaaa ccttcccctt attgtgggtg
     1441 tgattgtttt tagccagctg ttaattgatg cggggcttta tatttattta aataaaacca
     1501 tgcagtcttc acttcttcgt atgcaatatt aaaaggagga attatgattg tagtaaaagg
     1561 aacttataac agtgccaagg tatttactaa tgacctagag gatacggcgg ccaagcaaat
     1621 aaaagaactc ttagaacaag aatttgttga ggggctaaaa atccgtatta tgccagatgt
     1681 tcatgctgga gcgggctgca ccataggaac gaccatgacc cttgaagata aggtggttcc
     1741 caaccttgtg ggcgtggata ttggctgtgg tgtggaggtt gttaaactag gaaaacaaaa
     1801 gcctgattat cgctctctgg atgagctgat atacagagga attccctcag gctttaagat
     1861 aagaaaaaga gagcatcctt ttttaaaaaa tgtagctata gatagactta tttgcaaaaa
     1921 taaggcaaaa ctcaatatgg atagagcaaa gaaaagtcta ggcaccctag gagggggaaa
     1981 tcattttatt gaaataggag aaaatgaaaa gggagagttt tatctgttaa tacattcagg
     2041 tagccgaaat attggcctac aagtagccct atattatcaa aaacttgccc agaaaaaaca
     2101 aaaaggaaaa ggaattccaa gagatcttac ctatctagaa ggaaaagaca ctgagaatta
     2161 tctccatgat atggccctta tgcaaaacta tgctgagtgg aaccgccgag ccatggccca
     2221 gattatccta gatggactag gctatgagaa cttagggcgc tttagtacca tccataatta
     2281 tattgatatt gaagaagcta tcctgcgcaa gggagccgta agtgcaaaaa aaggagaaat
     2341 cttccttctt cctatgaata tgagagatgg ctcctttatt tgtagaggaa agggcaatcc
     2401 cgattggaac tattcagccc cccatggagc aggtcgtatt ttgtctagaa cccaggcccg
     2461 gaaaaaactt tccttaaaag aatttaagac ttctatggag ggaatttata ctacttcagc
     2521 tcaaaataga accttagacg aggccccaag ggcctataaa aagatgcatg aaatcctaga
     2581 aaatgttgaa cccaccttag aggtccttca tcatttgaag gttaggtata actataaggc
     2641 aacgaattag acactaaatc atggaggaaa gaatgtttat atctcgagtg gaaaaaacaa
     2701 gagtgaaaat tggccatatc ccagccctag tatttgaacc aatagataaa aaggcccagg
     2761 ggcatattat ccattatcac ggctggggta gtaaaaaaga aaatcatagt ctagtggcct
     2821 cggctcttgc tgcagcaggt tttatggttc ttgttcctga tgggctttat catggagaaa
     2881 gaggtccctt tgaagaagaa gatgagaccc ttcttcctga tgtaatccta tcaaatctta
     2941 gggagtttcc tcttttgtct tcttatttaa aggcagaaaa actctatctt accggtcact
     3001 ctatggggag tatgagcgcg ggagttattt ttcatcagga aaaaacagtg gatgcagcta
     3061 ttattattaa tggataccta agctataagg accttgatct tgatatggag gttccaaaag
     3121 aacttcttga agttgatcca atcgattttc ttgcaaatgt gaaagatcgc catcttctta
     3181 ttctccacgg tgatgccgat agttctgtag atataaaggt gcaaagagaa tacgtaaaaa
     3241 aagcccaggc ctactataag cctggacatc tgattatgga agaaatccct agcctcaatc
     3301 actatgtaac tgtagccatg ctagctagaa tgatagacta tttaagtcat ctttaaggcc
     3361 cttctaaagg gcttttttta tccaagtttt ctagcagcct ttaatgtgcc agccaagctt
     3421 ttggctgttt tttctaggtt tttttgtgtc atcttgggct ccatagcatc ttcaaggctt
     3481 atgggcccct gctggataga aaaacttcca tcaaaaagag ggctttctgc tataagttca
     3541 ccaaaaaatc caaatacggc cttatttttt tcttttgctt ttcgggccag ctccgtgggt
     3601 cctttgcctt ggaggctctg ggaatcaaat cgaccttcac cggtgataac aatatcagcc
     3661 catagcagat gatcattaaa attacatagt tctgttagta aagaaaaacc tggcttaagg
     3721 ctggctccaa taagtagaag agcaaagcca agcccgccag ctgctccagc accgggaagg
     3781 tcctttactt ttttttgaaa atgcttttct agaagcaggg catagccttt catggcctga
     3841 tctaccctag gaagatcttt tgcctttaat cctttttgag gaccatagac ggcgctggcc
     3901 ccattttctc ctaagagagg atttgtaaca tcaacagcga caagaagatt tgagatagag
     3961 gcgatctccc cttctatgcg gtttacataa aataaacttt caagagaggg gctaagctcc
     4021 ttgtcatcat ggtaaaagcg cagccctaga ctatgaagaa aacccatgcc cccatcattc
     4081 acccctgttc ccccaaggcc aagaaggatt ttttttggtg aaagactgcg tgcaataagc
     4141 agctccatgc caaagcccaa gctactggcc ttatagatat cggttttttc ttgtaggccc
     4201 gcagcatggg cggcttctaa aaaagcaatc tcctggttat agtatatttt ggactttagg
     4261 ggaagaccat agaggtctac tatttcttcc tggtgcagat ctaggataaa aaaatgatta
     4321 agcatctcca gggttccctc gcccccatcg gctagaggaa gaaggcgaat ctgactctta
     4381 gtaagactag agcggagtcc tttttctacg gctctagcag cctcaagact agatagactt
     4441 cccttaaaag aatcaatagc aataagaata ttagacttca tgaatgggcc ttccttccaa
     4501 aaaagctttg agattatcaa ggctgctatc cataattcgt tttcttgctt cccttgtaga
     4561 ccaggcaata tgaggggtaa gaatcaggtt ttccactcca agaagaggag aatctttttc
     4621 catgggttca agatttacta catcaagagc ggctccggca aggaggcctt tttttagagc
     4681 atttaccaga tcttcttcta caataagggg gcccctagag cagtttataa taaaggctgt
     4741 ttttttcata agtcccaggg tgttttcatt taccactccc aggctctgtt tattcaaaga
     4801 actatgaagc gataaaaaat cactttcttt aaagaggtct tctaaagaaa caaaagaata
     4861 ggggacgtct tttttacttc tagaatgata gatgatctcc atgcctagag cctcagcgat
     4921 tttagcaaag gcaaggccta tgcgacccag gccaaaaaga cctaaagttt ttccggcaag
     4981 ttctatttgc ggggagagaa gataggtcca gtcatcgcta cgtgtccagt ctcccctatg
     5041 gacggatttg ctatgcctac ctacctgatg ggaaagctct agcatcatag ccgtggcaaa
     5101 ttgggccaca gtaagggttc catagctggg aacattgctg ataataatcc cccgggcctt
     5161 agcggcctgg atatcaacaa tattgtagcc tgtggccagc acgctgataa atctcacctt
     5221 agaatcttcc agggcttttt catcaatagg agttttgttg gtaaagacga tgctagcatc
     5281 tgctattctt ttttttacat cttctttttt tgttctgggg tagacagtga gctgtccaaa
     5341 ttttcttagt tcatcccagc taagatctcc gggatttaga gcgtgaccat caaggacgac
     5401 gagcttcatt tttttctcct ttcagagcgc tatacataag gataaaggca agtatttgaa
     5461 taaaagaaac cgattgcaaa agaaccctaa taccaaagct gtccatcagt atgccaccta
     5521 taaaagaagc cagcacattt cccgcaataa tagcagaggt cagtagagct tgtcctgttg
     5581 ccgcatcttt tttttccata gtatgggata gataaaaaat ggcagtagga agaaaaagac
     5641 caaaggttac cgcattaagg ctttgattta tatatagcat atatatgttt tttgcaaaga
     5701 gatccagtag agcctttaag gcgaaaaaga aggcagaaaa gagtaaaagc tttttttctt
     5761 caaagcggct agaaatccaa gtaaaagaaa acatagttgg tacttctgaa aaagctgaaa
     5821 tacccaaggt aaggcccagg gcagactcat cgcctccaac aaagcggata aggtggatta
     5881 gataactatg aaacatgcca tagccgcaaa agagcaggac aacggcaggt aaaaagagat
     5941 aaagaaaagg atagcgcttt gcgatattac ctttgatatc tccctctttt ttatagaagg
     6001 cagggaaggg aaagctcagc acacaaagta tatatataac gataaaaacc ataccagcat
     6061 aaggggtaat aaaaatcccg cgtcttgcaa gaagattacc taaaaacacc gaaccaagag
     6121 aataaagcaa agaggccatc cccctggaaa caccaaaatc gagcgggata tcctcatgaa
     6181 ggcaagcaac acttaaggcg ttaacaaagg gctgcagact cataagaaaa agaagcagta
     6241 gaaaatagaa gggagcaagg gcttgccagg caactagtcc tgccactaat aaaatggcaa
     6301 atccctgggt gagcataagt cccttcaaag ggatcttgcc attatctacg gcggaagcta
     6361 aaaaagattg aaggggaatc gaagcaaggt tggccgaagc aaaaagaagt ccaatgccaa
     6421 tattgctata gccagcatca agtagaaagg ttgtggat
//
LOCUS       DASRNA010000055         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_387830,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000055 DASRNA010000000
VERSION     DASRNA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387830"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFD08_01645"
     CDS             complement(<1..575)
                     /locus_tag="VFD08_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HZK09750.1"
                     /translation="MSNLFERFRKSLNKTSKNLATKIDTLFGYYSEVDDEFFEELEEI
                     LILADIGFETTEAVLEQLQEEVYERILSSPSEVKPLLEEILISKISSEKIFDRTNPPR
                     VILVIGVNGAGKTTSIGKLSNLLVEEGKSVLLAAGDTFRAAAIEQLGVWAQRSGVDMI
                     RHQEGSDPASVIYDAIQAAKSRGTDVLICDSA"
     gene            complement(572..>2732)
                     /locus_tag="VFD08_01650"
     CDS             complement(572..>2732)
                     /locus_tag="VFD08_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09751.1"
                     /translation="EELLTSFLALEQAKKSLEEEKLPLERKVLRHKRQKAELEEDKKL
                     FEEKMSFLQTKKAETASQISALAKNISLYRKWQEEGDKNLEEARKLLGGEILYKKIRP
                     KKGYEKALEAALAQGLNSLDLRGVPSSTWMKKLGRSSVNLIYQELSPLKARDEKSLGS
                     LENFVEGPRDLCENLLGGILVYPSLEEALEGPKDLIRVTLKGERLEKGFYYGSENAAS
                     PLEISIKLEEGLAEKEELQAALEEVQKEIYETTHFGREKQKELKDLLLAEEILAKEQK
                     SLKDKENLLYSEERLSKFKKEQSQEAITRQKARLEELVAKHKDLKAQIKNLKKEEENV
                     KEMEGTSELESLDELRQKSLTLRLEEARSLEEVKRWEEEIYQLELGRQENKKRQEEKK
                     EKKDNSIKENRQLLAELDSLREIYAREQSEIKRLDEQAFSIKKEIDLVRNKLPELEKT
                     RSLLEEELYLLHDEQSKKEIRLSRVEERKNALSEEIWRKYELSMLQIKDLDLSCEEPR
                     SYRKKIRQEMLELEPVNLKAPEEFEEVDERHGFLSVQLADIAASKKDLETTIDSLEVR
                     MVEEFTLTFSAIRENFKDIFSHLFRGGQGDILLEDPEDVLASDILILASPPGKKLQHM
                     NLLSGGEKSLTAIALLFAVLKTKPAPFYVLDEIEAALDDINIQRFGTFLEEFSEGSQF
                     ILITHRKGTMEFADALYGVSMEEKGISSLVSLKMEES"
BASE COUNT          653 a          609 c          485 g          985 t
ORIGIN      
        1 gcactatcac aaattaatac atcagtgccc ctacttttag ctgcctgtat ggcgtcgtag
       61 ataacactgg ccgggtcgct tccttcttga tgacgaatca tatcaacccc agaacgctgg
      121 gcccaaacgc ccaactgctc aatggccgct gctctaaagg tatcgcccgc agcaagaaga
      181 actgattttc cctcctccac taaaagattg gacagttttc ctatggatgt agttttgcca
      241 gcaccattaa caccaataac aaggatgaca cggggagggt tagtgcgatc aaagattttc
      301 tcggaagaaa tttttgatat tagtatttcc tctaataggg gctttacttc actaggggat
      361 gaaaggatgc gctcataaac ctcttcttgt agctgctcta agacagcctc tgttgtctca
      421 aagccaatat cagctaaaat tagaatttcc tctaattctt caaaaaactc atcatctact
      481 tcactatagt aaccaaaaag agtatcaatt tttgtggcta ggtttttact tgttttgttt
      541 aaagattttc taaaacgttc gaataaatta ctcatgattc ctccattttt agggacacta
      601 gcgatgagat acccttctcc tccatagaaa ccccatacaa ggcatcggca aattccatgg
      661 tcccttttct gtgggtaata agaatgaatt ggcttccctc agaaaactcc tccaggaagg
      721 tgccaaaacg ttgaatatta atatcatcta aggcggcttc aatctcatcc aaaacataaa
      781 agggggcagg ttttgtcttc agtaccgcaa aaagtaaagc aatggctgtt agggattttt
      841 ctcctccact aagcagattc atatgttgga gctttttacc agggggtgaa gccaggataa
      901 gaatatcact tgctagtaca tcctctggat cttctagaag aatatctcct tgccctcccc
      961 taaaaagatg actgaaaata tctttgaaat tttcgcgtat ggcagaaaaa gttagtgtaa
     1021 actcttcgac catcctaact tcaagggaat ctatagtcgt ttcaaggtct tttttactgg
     1081 ctgcaatatc tgctaattga acactaagaa agccgtggcg ctcatctact tcttcaaatt
     1141 cttccggtgc cttaagatta accggctcta gttctagcat ttcttgtcgg atttttttac
     1201 ggtaactgcg gggctcctca cagctgaggt ccaaatcttt tatctggagc atactcaact
     1261 catattttct ccaaatttcc tcagaaagtg catttttacg ttcttccact cgactaagac
     1321 gaatttcttt tttactctgc tcatcatgaa gaagatagag ttcttcttct agtagacttc
     1381 ttgtcttttc aagttcagga agtttatttc ttacaagatc gatttccttt tttatgctaa
     1441 aggcttgttc atcaagcctt ttgatctcac tttgttctct tgcgtagatt tctcttaagc
     1501 tatctagttc agcaagtagc tgacggtttt ctttgatgct attatctttt ttctcttttt
     1561 tttcttcttg tcttttttta ttttcttgcc tgccaagttc aagctggtaa atttcttctt
     1621 cccatctttt aacttcttct agggagcgag cttcttcaag gcgaagggtt agactctttt
     1681 ggcgaagctc atccagactt tctaattcag aagtaccttc catttccttg acattttctt
     1741 cttctttttt taggtttttt atttgagcct ttaggtcttt atgcttcgcc accagttctt
     1801 ctagtcgagc cttctgcctt gtaatggctt cttgactttg ttctttttta aacttgctca
     1861 ggcgctcttc gctataaaga aggttttctt tatcctttag actcttttgc tcttttgcca
     1921 aaatttcttc agctagaagc agatctttta gctccttttg cttttctctg ccaaaatggg
     1981 tggtttcata aatttctttt tggacttctt ctagggcagc ttgaagttct tctttttctg
     2041 ccaacccttc ttctagcttg atacttattt caaggggact ggctgcattt tcactgccat
     2101 aataaaatcc tttttctagg cgttcgcctt taagggttac tctgattaag tctttgggtc
     2161 cctccaaggc ctcttctaga cttgggtaga ctaaaatccc tcccagaaga ttttcgcata
     2221 aatccctggg cccttctaca aagttttcta ggcttcccag gcttttttca tcccttgcct
     2281 taagggggga aagctcctgg tagattagat tgacagagct tcttccaagt tttttcatcc
     2341 aagtagaaga gggaacgcct cttaggtcca aactattaag accctgggct agggctgctt
     2401 ctaaggcttt ttcatagcct tttttcgggc ggattttttt ataaaggatt tctcctccta
     2461 gaagttttct ggcttcttct agattcttat ccccttcttc ttgccacttt ctatagagac
     2521 taatattctt tgcaagggct gaaatctgtg atgcggtctc ggcctttttt gtttgtaaaa
     2581 aactcatctt ttcttcaaag agttttttat cctcttctaa ttcagctttt tgtcttttgt
     2641 ggcgtagcac ttttctttct agaggaagtt tttcttcttc tagggacttt tttgcttgct
     2701 ctagtgccaa aaagctagta agaagttcct ca
//
LOCUS       DASRNA010000056         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_317326,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000056 DASRNA010000000
VERSION     DASRNA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317326"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFD08_01655"
     CDS             complement(<1..638)
                     /locus_tag="VFD08_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZK09752.1"
                     /translation="MNFSDSNKKTVVIFGDSNTYGYNPETEGRYPESYRYPYVLQELL
                     GKEWEVISQGLNGRTSVFEDPLDEGLSGITYISPCLKTDKPIDKLIIMLGTNDSQERF
                     NVGAVHIAEGIVNLAEKAAQTPCWRKDPDILILCPPPIKDNYRELKFGLEMGEACDVK
                     TAQLAYFLETMAIEGRFSFLDAGLVPGVEMHPLDGMHLTAESHVALAQFLLK"
     gene            complement(716..2149)
                     /locus_tag="VFD08_01660"
     CDS             complement(716..2149)
                     /locus_tag="VFD08_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZK09753.1"
                     /translation="MRPIVPYFLSRILQLEGILLLLPLLVSLIYREPFAYWGSFLGTA
                     ILVFAIGKLLSLFQPKDERIYAKEGLILASSSWLLMSIFGALPLWLSGDYPHFLDALF
                     ETISGFTTTGATVATAVEDLGHSILFWRSFTHLIGGMGVLVFALAVFPSGRGSIHMAK
                     AEMPGPSFSKVVAKLSETARILYMIYLAMTVILTILLLIAGMPLFDSLCHAFATAGTG
                     GFGIKNTSIAFYQSNSIESILAVFMILFGINFNLYFLLMMGQVKKFWKSEELRFYLGL
                     IFGATVLIFIQTHRYSGKALLDSFFTVASIITTTGFSTANFDIWPLLSRGILLLLMFI
                     GAMAGSTGGGLKVSRTVILGKSGFRELIQARNPKRVIPLRFENQAVSRDQLRSISSYM
                     SMYTLIFIGAFFILLLDLPNFISAFSAVAATINNIGPGFDIVGPTGNFASLSYLSKTT
                     LSFCMLAGRLELWPVLVLFSPSTWKKI"
     gene            complement(2159..>3160)
                     /locus_tag="VFD08_01665"
     CDS             complement(2159..>3160)
                     /locus_tag="VFD08_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZK09754.1"
                     /translation="LILNPDLQAASEIARLLQFPTALSIEPFAHNRVNMVEIEIPDDH
                     DFIGKNLITFRESCPDVLVCVVVREGVPFIPYGSTILQANDHLFITGTIAKLRAVYRM
                     FADSNDPLRSILIVGGGRITFYVLKRLAGQGFDIKVIEQDAELARELSEDFPDVHVVH
                     GDGTDQELLEEEGIRYFDFTLALTGVDEENILISLFGISQEVPRNITKVNRLRLLRVL
                     QNMDLQSIITPHEIVATEIIRTVRALNQDNESNMEAMYRIAQGQVEAMEFHVVQASNV
                     CGRNLADLPIKRGILVAYIIRGPELIVPGGKDFIEPGDHVIVVTNGHAIVNIDDILD"
BASE COUNT         1169 a          669 c          542 g          780 t
ORIGIN      
        1 tatttcaaca aaaattgagc taaagccaca tggctttcag ccgttaaatg catcccgtct
       61 agtggatgca tttcaactcc aggaacaaga ccagcatcca gaaaagaaaa acgaccctct
      121 atagccatag tttctaaaaa ataagctaat tgagcagttt taacatcaca agcctctccc
      181 atttctagac cgaactttaa ctctctataa ttgtctttga tcggtggtgg gcaaagaatc
      241 aatatatctg gatcttttct ccaacaagga gtttgagcgg ctttctcagc caaattaaca
      301 ataccttcag caatatgcac tgcccccaca ttaaagcgtt cttgactgtc attagtgcca
      361 agcatgatga ttagtttatc tataggttta tctgttttca gacaaggaga gatataagta
      421 atcccagaca aaccttcatc taacggatcc tcaaaaacag aagtgcgtcc atttaagcct
      481 tgacttatca cttcccactc tttgcctaaa agttcttgta aaacataagg ataacgataa
      541 ctctcaggat agcggccttc agtctctgga ttatagccat aagtattgga atcaccaaag
      601 atgacaactg tctttttatt tgaatcagaa aaattcatgt ttacctcaaa tcaattaaac
      661 taataaatag ctattgatct cagcttttat aagtgtaatt ttgtactttt atcttttaaa
      721 tcttcttcca agtcgaagga gaaaacagta ccaaaactgg ccacaactct aaacgaccag
      781 ccaacataca aaaagaaaga gttgttttag acagatagga taagctagca aagttacctg
      841 ttggaccaac aatatcaaaa ccaggaccaa tattattgat agttgcagca acggctgaaa
      901 aagctgaaat aaagttaggt aagtcaagca agagaataaa gaaagcccca ataaagatga
      961 gcgtatacat actcatataa ctgctaatag agcgaagttg atctctgctt actgcttgat
     1021 tttcaaatct caaaggaata acacgtttgg gatttctggc ttggattagt tcgcgaaaac
     1081 cacttttccc taaaataacc gttcgagaaa cttttaaacc tccgccagta gaaccagcca
     1141 tagcccctat aaacataaga aggagcaaga tacctctcga taaaagtggc caaatatcga
     1201 aattagccgt agaaaaacct gtagttgtta taattgaagc aaccgtaaag aaagaatcta
     1261 agagagcttt acctgaataa cggtgcgtct gaataaagat taaaaccgtt gctccaaaga
     1321 taaggcccaa ataaaaacga agttcttcac ttttccagaa ctttttaact tgacccatca
     1381 taagaagaaa ataaagatta aagtttatcc caaaaagaat cataaagaca gccagaatcg
     1441 actcgataga attagactgg taaaaagcta ttgaagtatt tttaatcccg aaaccacctg
     1501 tacctgccgt tgcaaaagca tgacaaaggg aatcaaaaag aggcataccg gcaatcagca
     1561 agaggattgt cagaatcacc gtcatagcta aataaatcat ataaagaatc cgagctgttt
     1621 cagataactt agcaactacc ttagaaaaag aaggaccagg catttctgct ttagccatat
     1681 gaatagagcc cctaccagaa gggaaaactg ctaaggcaaa gaccaaaacc cccataccac
     1741 caattaagtg agtgaaagaa cgccaaaaga gaatagagtg gcctaaatct tcaacggctg
     1801 tagctactgt agccccagtt gttgtaaaac cagaaatggt ttcaaagaga gcatcaagaa
     1861 aatgtgggta atcaccagac aaccataaag gcaaggcccc aaagatagac ataagaagcc
     1921 aggaagaaga agctaagatc aaaccttctt tagcataaat ccgctcatct ttgggttgaa
     1981 aaagagacaa taatttccct atagcgaaaa ctaaaatagc agtgcctaaa aaacttcccc
     2041 aatatgcaaa aggttctcta taaattaaag aaacaagcaa gggcagtaaa agaagtattc
     2101 cttcaagctg caaaattcga ctaagaaagt agggtacgat aggtcgcata gtaatctcct
     2161 agtctaagat atcatcaata ttgacgatag catgtccatt ggtaaccaca atgacatgat
     2221 cgccaggctc aataaaatct ttacccccag gaacaatcaa ttcaggacca cgtataatat
     2281 aagcaaccaa aataccacgc ttaattggta agtcagctaa attcctgcca cagacattag
     2341 aagcttggac cacatggaac tccatggcct ccacctgacc ttgagcaatc cgatacatag
     2401 cttccatatt agactcattg tcttgattta aggcccttac tgtacgaata atctctgtcg
     2461 ccacaatttc atgaggagta ataatacttt gtaaatccat attctgcaaa acacgcaaaa
     2521 ggcgcaaacg atttaccttg gtaatattac ggggaacttc ttgagaaatg ccaaaaagag
     2581 aaattaaaat attttcttca tcaacaccag ttaaagccag cgtgaaatca aaataacgaa
     2641 tgccttcttc ttcgagcaac tcttgatcgg tgccatcacc atggaccaca tgaacatcag
     2701 gaaaatcttc acttaattca cgagccaact ccgcatcctg ttcaatcacc ttaatatcaa
     2761 aaccttgtcc agccagacgc tttaacacat agaaagtaat cctaccgcca ccaacaatta
     2821 aaatagaacg caaaggatca ttactgtcag caaacatgcg ataaacagca cgcaacttag
     2881 caatggtacc agtaataaaa agatgatcat tagcttgtag aatagtagaa ccataaggaa
     2941 taaaaggcac tccctcacgc acaaccacac aaactaaaac atcgggacaa gattcacgaa
     3001 aagtaatcag atttttacca ataaagtcat gatcatcagg tatttcaatt tctaccatat
     3061 tgacccgatt atgggcaaaa ggctcgatag ataaggcagt agggaattgg agcaagcgtg
     3121 caatttcaga ggcagcttgt aaatcaggat ttaagatcaa
//
LOCUS       DASRNA010000057         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_93532,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000057 DASRNA010000000
VERSION     DASRNA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93532"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFD08_01670"
     CDS             <1..418
                     /locus_tag="VFD08_01670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZK09755.1"
                     /translation="HKFHLENEKYILFSLFKKAEEKEGDMVGYAMVEVDLLPKTYLLR
                     RIAFREKDKGYGRSALCALIKHCFDELELNRFYLDVYPHNKRAIYLYEDIGLVWEGTL
                     RENYYYDGVFLDQRLYSMLKREYKMTPLYHKSYQDL"
     gene            427..1629
                     /locus_tag="VFD08_01675"
     CDS             427..1629
                     /locus_tag="VFD08_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HZK09756.1"
                     /translation="MDLFALAIIFVLLLILMSRGLPLSLAMGASTILGFFLFRIPPAQ
                     ALGVVYQGVFSRTTLNLVLAFYTITYLQRMLEEREHLHLAESSLTRLFGSRRINAMIA
                     PFVIGLLPSVGAVLIARPIVDNAAQEDLTVEERCFVTSFYRHISEIFLPTYGSIILAM
                     EFSGVDMASFVFAMLPLVFLLFFLGFVFYVRKIPPTKAPTGVDKKREGKNIFISLWAI
                     ALSIVLILSFNIPVHLAILPVIILSVFINRFKWKEIRPLFRSAFQGRLILTTLAIMVF
                     KELLTFSGVIYSLPELFSNMPLSPVIIFGLIFFVGTLMAGAQAMIATMLPLAVATLGS
                     SLGLIVFLMSLVFIANQVTPIHVCLGVITEAYKLPFTSLVKKTLPVLLSFVIISSLYS
                     YLLFFIFN"
     gene            1678..2211
                     /locus_tag="VFD08_01680"
     CDS             1678..2211
                     /locus_tag="VFD08_01680"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZK09757.1"
                     /translation="MKIYDFQVLDMDEKEVSLDKYKGKVLLIVNTATRCGFTPQYEGL
                     EKLYQKYQEQGFVVLGFPCNQFLNQAPEDNEAIKGFCSLNYGITFPQFAKIKVNGDKA
                     HPLYKFLKEEKPRDLENPNTSGFLKKIKKLGVEPLGSAIRWNFTKFLIDREGNVVERY
                     APLVEPEELEEAIEKLL"
     gene            complement(2271..3443)
                     /locus_tag="VFD08_01685"
     CDS             complement(2271..3443)
                     /locus_tag="VFD08_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HZK09758.1"
                     /translation="MNFEGTMVDRKTCYSVKYKKMEEEGVKEGIPLWVADMDLPVAPA
                     ITEALIQRAKHPYFGYTYIPRKYYEAFAHWQNKRLGLELDIDLISPAHNVVVGLQLVL
                     EMFRQPGDNVLVQPPVYFPFYSMIEKVGCGRIDNPLIETDQGYFMDFEDMEKKIVDNN
                     VKFFILCSPHNPVGRVWNKQELERLADICYRHRVIVLSDEIHCDLIHQGQHIPFSTIS
                     DKAKEISIGFYSSGKTFNLAALHSAYVVFHSKHWQEKYEAISLAQNRGDANCFGIEAN
                     IAAFTKSEAWYQEMMSYLQGNIDYVEDFITSKLSPKIKVRKPQATFLMWLDCRGLGLN
                     SEELNHFFQEKAKLLLNEGHIFGEEGKGFMRLNIAAPRYLLEKAMEQLKEALDAVE"
BASE COUNT          972 a          690 c          685 g         1142 t
ORIGIN      
        1 acataaattt catcttgaga atgaaaagta tattttattt tccctcttta aaaaagcaga
       61 agagaaagaa ggagatatgg tgggttatgc catggtggaa gttgatttat taccaaagac
      121 atatcttctt aggcgcatag cctttagaga aaaagataag ggctatggaa gaagcgccct
      181 ttgcgccctt attaagcact gttttgatga gctagaatta aaccgcttct atcttgatgt
      241 ctacccccac aataaaagag ctatttatct atatgaggat ataggccttg tttgggaagg
      301 aaccttaaga gaaaactact actatgatgg tgtctttctt gatcaaagac tgtattcgat
      361 gctaaaaaga gagtataaga tgactcctct ttatcataaa tcctaccagg atctctagga
      421 gggaaaatgg atctttttgc tcttgctatt atatttgttc ttttacttat cttgatgagt
      481 cgaggtttac ctcttagcct tgccatggga gccagcacca ttttgggctt ttttcttttt
      541 cgaatcccgc cagctcaggc tttaggagtc gtttaccaag gagttttttc aaggacaaca
      601 ctaaatctag tccttgcctt ttataccatt acctacttgc aaagaatgtt agaggaaaga
      661 gaacatcttc atctagctga aagctcttta actaggctct ttggctctcg aagaatcaat
      721 gccatgatag ccccctttgt aataggactt cttccttcgg ttggtgcggt cctaatagca
      781 aggcccattg ttgataatgc ggcccaggaa gatcttacag tggaggagcg ctgctttgtt
      841 accagcttct accgccatat atcggagatt tttcttccta cctatggttc cattattctt
      901 gcgatggagt tttctggtgt agatatggca agttttgttt ttgctatgct tcccctggtt
      961 tttctgctct ttttcttagg ctttgttttt tatgttcgaa aaattccacc aaccaaggct
     1021 ccgactgggg tagataaaaa aagagaaggt aaaaacatat ttattagcct atgggctata
     1081 gccctaagta ttgtgcttat tttgagcttt aacattcccg tccatctagc gatccttcct
     1141 gtaattattc tttctgtctt tatcaatcgc tttaagtgga aggagataag acccttattt
     1201 cgctcagctt tccagggtcg acttattctt accaccttgg caattatggt ctttaaggag
     1261 ctcctaacct ttagtggtgt tatttatagt cttcctgaac tttttagcaa tatgccactt
     1321 tctcctgtta ttatatttgg acttatattt tttgtcggaa ccctaatggc aggagcccag
     1381 gctatgattg ccaccatgct cccccttgct gtggcaactt taggaagcag cctggggctg
     1441 attgtatttc ttatgagcct ggtctttatt gccaatcaag ttactcccat tcatgtatgt
     1501 ttaggggtta ttactgaggc ctataagctt ccctttacct cattggttaa aaaaaccctt
     1561 cctgtacttc tttcttttgt aattataagt tctctttata gttatctttt attttttatt
     1621 tttaactaaa gaaagaaaag ttgggtagaa atatcttaga cccataaagg aggatctatg
     1681 aaaatttatg actttcaagt gctagatatg gatgaaaaag aagtttcttt ggataagtat
     1741 aaaggaaagg tacttcttat tgtaaataca gcaacccgct gcggctttac tcctcaatat
     1801 gaaggtcttg agaaactcta tcaaaagtac caagaacagg gatttgttgt tttaggcttt
     1861 ccctgtaatc agtttttaaa ccaagctccc gaggacaatg aggcaattaa aggcttttgc
     1921 agtctaaatt atgggattac tttcccgcaa tttgcaaaaa taaaggtaaa tggggacaag
     1981 gcccatcctc tttataagtt tctgaaagaa gaaaagccaa gagatcttga aaatcccaat
     2041 acgtcaggtt ttcttaaaaa gataaagaaa cttggagtag aacccctagg ttcagccatt
     2101 cgttggaatt tcacgaaatt tcttatcgat cgtgagggca atgtagtgga gcgttatgct
     2161 cccctagttg aaccagaaga attagaagaa gccattgaaa aacttctatg ataataaaaa
     2221 aagcgctcct ttgtaaaaag gagtgctttt tatttgggcc ctataggagt ttattccact
     2281 gcatcaaggg cctcttttaa ttgctccatg gccttttcaa gaagatagcg gggcgctgct
     2341 atatttaggc gcataaagcc ctttccttct tctccaaaaa tatggccctc attaagaagc
     2401 agtttggctt tttcttggaa aaaatgattg agttcttctg agtttagccc tagtccccgg
     2461 cagtctagcc acattaagaa agtggcctgg ggctttctta cctttatttt tggtgaaagc
     2521 ttgctagtaa taaagtcttc tacatagtcg atattgccct gaaggtagct catcatttcc
     2581 tgataccaag cttcactctt agtaaaggca gcaatattgg cttcaatacc aaaacaattg
     2641 gcatctcctc ggttttgtgc tagagaaatg gcctcatatt tttcttgcca atgcttggag
     2701 tgaaaaacta cataggcaga atgtagagcg gccagattaa aggtttttcc cgaagagtaa
     2761 aagcctatag agatttcttt agccttgtca cttatagtag aaaaggggat atgctggcct
     2821 tggtggatta aatcgcagtg gatctcatcg cttagaacta taaccctatg gcgataacat
     2881 atatctgcta ggcgctcaag ttcctgctta ttccaaaccc ttcctacagg attgtgggga
     2941 ctacatagaa taaaaaattt tacattgtta tcaacaattt tcttttccat atcctcaaag
     3001 tccataaaat atccctggtc tgtttcaatt agggggttgt ctatgcgacc acagcctacc
     3061 ttttcaatca ttgaataaaa gggaaaataa actgggggtt gaactagtac attgtctcct
     3121 ggctggcgaa acatttcaag aacaagttgc aggcctacta ccacattatg tgctggactt
     3181 ataaggtcga tatcaagctc aagacccaag cgtttatttt gccagtgagc aaaggcttca
     3241 taatactttc tggggatata tgtgtagcca aaataaggat gcttagccct ttggattaga
     3301 gcctctgtga tggctggggc cacaggaagg tccatatcgg caacccagag ggggatgccc
     3361 tccttcaccc cttcttcttc catttttttg tatttaacgc tgtaacatgt tttcctatct
     3421 accatggtgc cttcaaagtt cataaactcc tcctttaatt aaaaatacct atatactatg
     3481 aattataca
//
LOCUS       DASRNA010000058         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_236998,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000058 DASRNA010000000
VERSION     DASRNA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236998"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFD08_01690"
     CDS             complement(<1..487)
                     /locus_tag="VFD08_01690"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09759.1"
                     /translation="MKRFFALVKKDFLLDYPYLVNPKTTFKDKKLRKKSLLQLFVFGI
                     LLVYLFIFMKPVFKLYDSYKAAGIPLAFLALGFMSYLFMLLVFSFPYILSKIYFSKDV
                     ETMLSLPLASEEILASKFLSLTFSSLIYAVFLVIPIMFKFGFAEKMGPIYYIYGFLGL
                     VL"
     gene            complement(484..1200)
                     /locus_tag="VFD08_01695"
     CDS             complement(484..1200)
                     /locus_tag="VFD08_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK09760.1"
                     /translation="MVHISNVSKSYAGKKAVDNISFDVDCGEIFGFLGPNGAGKTTTI
                     KMIVGLLAQDSGTIKIQGIDVEKNSLESKKLLSYVPDNPELYANLTGRAYLNFLADVY
                     GVSEEDRQRRIEKYGQLFEMQDSVDQFISSYSHGMKQKMAVMGALIHDPDLLIMDEPM
                     VGLDPKSAFRLKELMRERCDKKKSVFFSTHVMEVAEKICDRIAIISKGEIIAVGSMEE
                     IRKQARDKGSLEKIFLELTE"
     gene            1273..2493
                     /locus_tag="VFD08_01700"
     CDS             1273..2493
                     /locus_tag="VFD08_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZK09761.1"
                     /translation="MKKYLIPLVIIVLIAINFFFFNYYFPGSSLDGIDVSWQKRIEES
                     KIDLKETFTLDAPSLEQVFYLLEDGFTLQLLRPEKEIFSLKILHLISKETILYDKGRL
                     KEEVKELLNTLPDNQAPQDAQLLIKEGELVLLPHQLGYNFPDEDRLFEMIEDAISQEK
                     YQLDLEPFIDRPAVLSQDLQAEKNKWREYKLENEDYSWILEGKELYLLYNEDLSLNQE
                     KAQELLEDYFSEADSGNTIWMVDTEESLPLFIEALKNHDPSWEVVYRRVRRNPDSNYS
                     MYNGIAVSIDRQWLWMYRDSQVVFQAPIISGNPNKGYPTHRGQWSILSMDTNTRLANT
                     NREGYSYDVPVNYWMQINYEGMIEGIHDATWHWAFGTDTYLWDGSHGCINLSVADMAT
                     LYSLSWVGLPVWVY"
BASE COUNT          817 a          555 c          489 g          814 t
ORIGIN      
        1 gtaaaaccag ccccaaaaat ccatagatat aatagattgg gcccattttc tcagcaaaac
       61 caaatttaaa cataattggt atcactagaa aaaccgcata gattaaagag ctaaaagtaa
      121 gagacaagaa tttactagct aggatttctt ctgaggccag aggcagactc agcatggtct
      181 caacatcttt actaaagtaa atcttactaa gtatataggg gaaggaaaaa acaagcagca
      241 taaataaata agacataaag ccaagggcta aaaatgccag gggaatcccc gctgccttat
      301 aactatcgta gagtttaaat acaggtttca taaagataaa aagataaaca agaagaatcc
      361 caaaaacaaa aagctgaagc aaactctttt ttcttagttt tttatcttta aaggttgttt
      421 tcggatttac aagataggga taatctaaca aaaaatcctt tttaactagg gcaaaaaacc
      481 gtttcattct gttagctcca aaaaaatctt ctctagactg cctttatccc ttgcctgttt
      541 tcgtatttct tccatgcttc caactgctat aatctcaccc ttactaataa tggcaatgcg
      601 atcacatatt ttttctgcca cttccataac atgggtagag aaaaaaacac tctttttctt
      661 atcgcagcgc tctcgcataa gttcttttag acgaaaagca ctcttaggat caagtcctac
      721 catgggctca tccattataa gaagatctgg gtcgtgaata agggccccca tcacagccat
      781 tttttgtttc attccatggg agtagctgga gataaactga tctactgagt cttgcatttc
      841 aaaaagctgg ccatattttt cgatgcgccg ctgcctgtct tcttcactta caccgtagac
      901 gtcggctaaa aaattaagat aggccctgcc tgtaagattg gcgtagagct caggattatc
      961 gggtacataa ctaaggagct ttttactctc taggctgttt ttttctacat cgataccctg
     1021 gatttttata gtcccgctat cctgggcaag cagacctact atcattttta tggtagtggt
     1081 ttttcctgct ccatttgggc ccaaaaaacc aaatatttct ccacaatcta catcaaagct
     1141 gatattatct accgcttttt taccagcata ggatttgctt acattagaaa tatggaccat
     1201 tttttcaccc tttctattgt gcttttgttt aacttattgt accatacatt aagaaagacc
     1261 taaggagtcc ctatgaaaaa atacctgatc ccccttgtca ttattgtcct tattgctatt
     1321 aattttttct tttttaatta ttatttcccc ggctcaagcc tagatggaat tgatgtttca
     1381 tggcaaaaac gcatagaaga aagtaaaatt gatttgaaag aaacatttac cctagatgct
     1441 cccagcctag agcaagtttt ttatcttcta gaggatggct ttacccttca actacttaga
     1501 ccagaaaaag agattttttc tcttaaaatt cttcatctta ttagtaagga aactattctt
     1561 tatgataaag ggcggctaaa agaggaggtc aaggaactat taaataccct tcctgataat
     1621 caagcccctc aagatgccca actcttgata aaagaggggg aacttgtttt acttccccat
     1681 cagcttggat ataatttccc tgatgaagat aggctctttg aaatgataga agatgctatt
     1741 tctcaagaaa aataccaact tgatctggag ccttttattg atagaccagc agtccttagc
     1801 caagatctac aagccgaaaa aaacaaatgg cgagagtata agctggaaaa tgaggattac
     1861 tcttggatct tagagggaaa agaactctac ctgctatata atgaagacct aagcctcaat
     1921 caagaaaagg cccaggagct tcttgaagat tatttttctg aggcagattc tggaaatacc
     1981 atttggatgg tagatacaga agaaagcctc cccctattta tagaagccct aaagaaccat
     2041 gatcctagct gggaagtggt ctaccggcgc gtaagaagaa atcccgattc caactactct
     2101 atgtacaatg gaattgctgt ctctattgat cggcagtggc tgtggatgta tagagactct
     2161 caagttgttt ttcaagcacc tattatttca ggcaatccca ataagggcta tccaacccac
     2221 cgaggccagt ggagcattct tagtatggac accaataccc gccttgccaa tacaaatcgc
     2281 gagggatact cctatgatgt tcctgtcaat tattggatgc agattaatta tgagggcatg
     2341 atagagggta ttcacgatgc cacttggcac tgggcctttg gcactgatac ttatctttgg
     2401 gacggcagcc acggctgtat taatctttct gtggctgata tggcaaccct atatagcctt
     2461 agctgggtgg gcctgcctgt ttgggtttat taaaaaagat tcctgcttca gcgtgtaaaa
     2521 agagagtgaa ttcactctct tttttccttg ctccctacag gagcattttt tctttatcaa
     2581 gtagggctgc ttggcagtct atataataat cggtagactc ccccttcttt ttcaatggta
     2641 aatatcgttc ctatattcat ttctattgcc tgaac
//
LOCUS       DASRNA010000059         5952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_164734,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000059 DASRNA010000000
VERSION     DASRNA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164734"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1716)
                     /locus_tag="VFD08_01705"
     CDS             complement(292..1716)
                     /locus_tag="VFD08_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09762.1"
                     /translation="MKKLSVFFLLILVLLQAGCKEDKLISGSYLDTYEVVEDEVKLKE
                     QEQVNKLIPRYIEKNNRPYVIWNEVIDADQYRVSIKRESKAQPSYKTIRDRQIEVFPG
                     ETVFYQISPLTKDGKEMTDYLSDPLEVNYTIKGGYGGVEDWVRPDGFHTHRAKKEFPE
                     GMYWSTTLADEITSWIKETYSTQYLLGGHIAEIDRESGEWIAPISPSRRVDPQYEGSG
                     LDCVGFVNGFYYLYTQAFAGEDYKEMDLVMDETYGVYGSPFAPLAALGYSSHIVIKPS
                     LGGSYADKWHIRPGDINESDDHAWLISHQGDGFDSKIWEYEYMNGFSDHRTAESIRHD
                     SADNPHEYWAAAISNPLESYGKIKILALDKEGNPVEGGTYRLMDSYNRTMALEDQGSV
                     VELYRPNGDDFFNLIFARGESKDFYDEESTFTMLEAQTSLYRVIGGRYILEEIMPPEG
                     YMGLEPISIEIAPGEEKVIYLEYK"
     gene            2202..2543
                     /locus_tag="VFD08_01710"
     CDS             2202..2543
                     /locus_tag="VFD08_01710"
                     /inference="COORDINATES: protein motif:HMM:NF022953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HZK09763.1"
                     /translation="MKKWILLLFLLPYLLSGCSKKINMDNFINEPNFSGIVQEVSSHS
                     ILVKVNKEESELLSSDLISLSLDAKLEKSARNFNVGDEVRVYYNGNIAESYPAQVSEV
                     YAIILVNPARE"
     gene            2627..3304
                     /locus_tag="VFD08_01715"
     CDS             2627..3304
                     /locus_tag="VFD08_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HZK09764.1"
                     /translation="MKKIGMVVAVEIDAVLNKFGVPKKEIPFIAHSVYEYKLGEHTLF
                     VINSGVGQIAATMATQFLITEFEVDLIVNFGVVGGLTPEMEVTKTCIVESVVHYDFDT
                     SSLDKCEVGRYLEYPSIYIPTSKQIMDKALDIEPSLKKVVCASGDKFIDGKKEKERLH
                     QLYKADICEMEAAGIVLTCNKYKTPCLLIKTVSDGISGGAEEFVNTLHSTAETCLEIT
                     IAVLSEI"
     gene            complement(3332..4153)
                     /locus_tag="VFD08_01720"
     CDS             complement(3332..4153)
                     /locus_tag="VFD08_01720"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZK09765.1"
                     /translation="MYPEFAWEFLGNEISIKAYALFTVVGALGGVLVALPILRRQGVP
                     FRQSLGLFILMAPAFLIGARLLNYLVNPKAYGSNLKIYSFRLAGFSFYGGLLGALLAF
                     LFWQRRTKKPLWSLLDALVLPGVVAFSLARLGCFFNGCCGGKPTQSLLGMDFPIRGGN
                     EKLLSLFLGSLGQSTTHIKVYPTQLFELGLALVFLLPIYYLYLKRKLPKGGAFLIYAA
                     AFSAMRLMVLPLRNLPYPDLVKVFFYPLLYLSIIVLSILQCKKLQGAEKKASIKR"
     gene            complement(4154..4387)
                     /locus_tag="VFD08_01725"
     CDS             complement(4154..4387)
                     /locus_tag="VFD08_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09766.1"
                     /translation="MIKKIRLGITQENLRGKNRYFSIRFIAPNRMERGRGYYMLGKNK
                     GNLLSGYLRSRAFGEKTRSFIINPIDKKPLVNP"
     gene            complement(4550..5476)
                     /locus_tag="VFD08_01730"
     CDS             complement(4550..5476)
                     /locus_tag="VFD08_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019191.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HZK09767.1"
                     /translation="MKVKFISQKRILATLMVIIMVLGLVACSGEGTSTASEKEESLEE
                     FDKENAKKLAEENARYSNYSKTGLIELLEYEGLSSEAAKYGVESIEAGLDWKERAKNT
                     AETYLTYQAASEKGLREVLEYEGFEKDQVEFAIKSIEVDWKEQARKKALNYLEFQEFT
                     KDDLIVQLIYEGFSEEEAKWGVSKSEDLLAGAEEVPKEEELDQEAVFDIIEESFTDMG
                     DVRYIEESKTFTLLPTEEDTVDATLLLVSGDEDVLDSWLFMTESLRSMSEFVMDSLGS
                     GYSVHFLHPSNEDKVLLDLYDGEIKYNIADDF"
BASE COUNT         1861 a         1193 c          991 g         1907 t
ORIGIN      
        1 acactaaata ggggccttct ttactcagca gactcaaaga aatgaggagg agctttataa
       61 caaagatgag cttaactaga atctacaatc aagctagaca ctattagaaa agtctagctt
      121 aagatcgaaa aatcacctta aatttcaagg acgtagacct ttattataat agttcccatt
      181 tcttacaata ataaaaataa ggtgtatttt tttagctcta ccttactttt aaaagtaaat
      241 actccaactt taaaataaaa aaagagctcc caggctcttt ttctagaact cttatttata
      301 ttctaaataa atcacttttt cttccccagg agcgatttca atcgaaatag gctccagtcc
      361 catgtaaccc tctggtggca taatttcctc cagtatatat cggcctccaa ttactctgta
      421 aaggcttgtc tgggcttcta acatggtgaa agtggactcc tcgtcataaa agtcctttga
      481 ttcaccccta gcaaaaatca gattaaaaaa atcatcccca ttaggtctat aaagttcaac
      541 aacactcccc tgatcttcaa gggccatggt ccgattgtaa gaatccataa gacgataggt
      601 ccctccttca acgggattac cttccttgtc taaagcaagg attttgattt ttccataact
      661 ttctaagggg ttagaaattg ccgctgccca gtattcatga ggattgtcag cgctatcatg
      721 ccttatgctc tcagctgttc gatgatcaga aaatccattc atatactcat attcccaaat
      781 tttactatca aaaccatctc cttggtggga aataagccag gcatgatcat cggattcatt
      841 aatatctcca ggtcggatat gccacttatc agcgtagctt cctccaagac taggtttgat
      901 gacaatatga gaagagtagc ccagtgctgc taggggtgca aagggagaac cgtaaacccc
      961 gtaggtctca tccattacaa ggtccatttc cttgtaatct tccccagcaa aagcttgagt
     1021 atagaggtaa taaaagccat ttacaaatcc tacacagtca agtccgctgc cctcatactg
     1081 tgggtctact cggcgactag ggctaatggg agcaatccac tccccgcttt cacggtcaat
     1141 ctcggcaatg tgccccccta gtaaatattg tgtactatat gtttctttta tccaagatgt
     1201 tatttcatct gctaaagtag ttgaccaata cattccctca gggaattctt ttttcgcccg
     1261 atgggtatga aaaccatcgg gccttaccca atcttcaacg ccaccatagc cacccttgat
     1321 agtataattt acttccaagg gatctgaaag gtagtccgtc atttcttttc catccttagt
     1381 aagggggcta atttgataga aaactgtttc gcctggaaaa acttcaatct gcctatctct
     1441 aattgtctta taggagggct gggcttttga ttctcgttta atcgaaaccc tatattgatc
     1501 cgcatcaatt acttcattcc aaattacata gggacggtta tttttctcaa tatagcgggg
     1561 aataagttta ttgacctgct cttgttcctt aagctttact tcatcttcca ctacttcata
     1621 ggtgtctaaa taagaacctg aaataagctt atcttcttta catccagctt gtaataaaac
     1681 taaaatcaaa agaaaaaaaa ctgataactt cttcatattt atccttgcct tcctttaaaa
     1741 gcgtatttgc acattgatta aattatcttc gccttaactt taatgaacct tagaggaatt
     1801 gtcaattcgc attttgttct cgtaaagaaa actccaccaa taaggcttct ccaattaagt
     1861 aacctttgct tcttttttat tctctttgat gtcctaaaat aagaccctat aggttttttc
     1921 cccaaataaa ttactcagta aacagtctag taaaacaagc taaggtggta atgattataa
     1981 aaactcatat gtggtagtat ggtgatgaaa tcaaaaaaat attaagttta gcctctttaa
     2041 gtccaaaact caagttgagg aaagctcttt gtaaaagaca tgaaaaagct gatgaatact
     2101 aaaacttaga aaaacagtag taatatcaag ttttttttca tccactagtt taaatgaact
     2161 tgttttataa aaaaagataa ataagaaggt aggaaaatac gatgaaaaaa tggattcttc
     2221 ttttattttt attgccatac ctattatctg gatgttctaa gaaaataaat atggacaatt
     2281 ttatcaatga acctaacttt agtgggattg tccaggaagt aagtagtcac tctattttag
     2341 taaaagtaaa taaagaagaa agtgagcttc taagtagcga tttaatatcc ctttctttag
     2401 atgccaaact cgaaaagagt gctagaaact ttaatgtagg agatgaagta agagtctact
     2461 ataatggaaa cattgcagaa agctatcctg cacaagtcag tgaagtctat gctataattc
     2521 ttgtaaatcc cgcccgggag tagaacaact ataaatgaat tattttatca aaatccctag
     2581 agtcaccata ttgggaaact ttcaaaaaaa caaaggagct tctatcgtga agaaaattgg
     2641 aatggttgtg gctgttgaaa ttgatgctgt attaaataag tttggggtcc caaaaaaaga
     2701 gatccccttt atagctcatt cagtctatga atacaaatta ggagagcata ccctatttgt
     2761 tattaatagt ggagtcggtc aaattgctgc tactatggca acgcaatttt taataacaga
     2821 atttgaggtg gatttaatcg tgaattttgg tgttgtaggg ggcctaactc ctgaaatgga
     2881 agtaacaaaa acatgcatcg ttgagagtgt agttcattat gactttgata catctagtct
     2941 tgataaatgt gaagtcggta gatacctcga gtatcctagt atttatatcc ctaccagcaa
     3001 gcaaataatg gataaggccc ttgacattga gccatcttta aaaaaagtag tatgtgcttc
     3061 tggagataag tttatcgatg gaaaaaaaga aaaagaaagg ctacaccagc tctacaaagc
     3121 agatatttgt gagatggaag ccgcggggat tgtattaacc tgtaataaat acaaaacacc
     3181 ttgtttactt ataaaaactg tgtccgatgg cattagtggt ggggcagaag aatttgttaa
     3241 cacacttcac tcaacagcag aaacttgttt agaaataaca atagcagttt taagtgagat
     3301 ttaggctcca aaatgaaaga agcctaatcc cctatctctt aatagaggcc tttttctcgg
     3361 caccctgcag tttcttacat tgtaggatgc ttaaaacaat aatacttaga taaagtaagg
     3421 gataaaaaaa cacctttact agatccggat agggtagatt ccttagagga aggaccataa
     3481 gacgcatggc agaaaaagct gctgcataaa taagaaaggc ccctcctttt ggaagttttc
     3541 gcttaagata aagatagtag atgggcaaaa gaaagactaa agcaagtccc agttcaaaaa
     3601 gttgggtagg gtaaacttta atgtgtgttg tgctttgccc taggctgcct aaaaatagag
     3661 acaggagttt ttcatttcct cctctgatgg gaaagtccat ccccaaaagg gactgcgttg
     3721 gcttaccccc gcaacatcca ttaaaaaaac atcccaatcg agccaaagaa aaggctacta
     3781 ccccaggtaa aacaagagca tctaataggg accacaaggg cttttttgtt cgcctctgcc
     3841 aaaataaaaa ggcaagcaag gctcccaaaa gtcccccata gaaagaaaac cctgccaatc
     3901 gaaaagaata aatctttaga ttactgccat aggctttagg atttaccaga taatttaaga
     3961 gccgggcccc tattagaaag gccggtgcca ttaaaataaa tagcccaagg gattgtctaa
     4021 aagggacgcc ttgacgccta agtattggca aagctaccag cactccaccc agtgcaccta
     4081 ctactgtaaa gagtgcatag gctttaattg aaatctcatt tcctagaaac tcccatgcga
     4141 attctggata catttatgga tttaccaagg gctttttatc tattggattt attataaagc
     4201 ttctagtctt ttctccaaag gccctacttc ttaaataacc acttagcaaa ttccctttat
     4261 ttttaccaag catatagtac cctctgcccc tttccatcct attaggtgcg ataaacctaa
     4321 tagaaaaata tctattcttt cctcttaaat tttcttgtgt tatcccaagc cttatctttt
     4381 taatcaatga ctagtagtat atcccagctt attcttcttt ttacattttt ctcctataaa
     4441 ggaaattagc ccttagatta caagggcttt caatttattt gatggatttc ccacttctta
     4501 gtttagatat tcttcacttt ttgactctag actatcgttt tactagtaac taaaaatcat
     4561 ctgcgatgtt gtattttatt tcaccatcat aaagatctaa cagtaccttg tcttcattag
     4621 atggatgtaa aaaatgtacg gagtatccac tacctaatga gtccattaca aattcagaca
     4681 tagagcgcag cgactcagtc ataaaaagcc aagaatctaa cacatcttca tcccctgaaa
     4741 ctaacagaag tgttgcatca acagtgtctt cttctgtagg aagcagggta aaagttttgc
     4801 tttcttcgat atatcttaca tcccccatgt cggtgaaact ctcttctatt atgtcaaaga
     4861 ctgcttcctg gtctaattcc tcttctttag ggacctcttc tgctcctgca agaaggtctt
     4921 ctgatttact tacaccccac tttgcttctt cttctgaaaa tccctcgtaa atcaattgaa
     4981 ctattaggtc atcttttgta aattcttgga attctaaata gttcaatgct ttttttctag
     5041 cttgctcttt ccagtcaacc tctatactct ttatggcaaa ttctacctgg tctttttcaa
     5101 agccttcata ttccaggact tctctaagac ctttttctga agcagcctgg taggttaaat
     5161 atgtctctgc tgtatttttt gctcgttctt tccagtcaag accagcttct atactctcta
     5221 ctccatattt tgctgcttct gaactaagac cctcatattc aagtaactct attaggcctg
     5281 tctttgaata atttgagtat cttgcatttt cttctgctag tttttttgca ttttctttgt
     5341 caaattcttc taaagattct tccttctctg aagcagtgct tgtaccttcg ccactacagg
     5401 caacaagacc aagaaccata attattacca tcaaagtggc cagaatcctt ttttgggaaa
     5461 taaattttac tttcattgta tcctcccctt taaatctaat tgctttttca aatcctttgg
     5521 aagtgttttt cccagggcct tttcataaag atatgtaatc tcatagtaag caagtgtttt
     5581 attggtctta ttcggctcgg ttatagggtt ttcacatctc atcctttttt tactacattt
     5641 cctcattata tatctgtttt atttatcata ttgtaccata cattattact ttttttaaac
     5701 tcttttaagc agtctgaaaa ataatgtgtt aaaaagagtc agctgcaagg tgaggaagta
     5761 aagcagtaaa gaattttcaa tggtttaaat aagagagtct taccaggctg aactaccaat
     5821 ttactaggtc tccatcaaaa ctaaaaccac ctactgattt aggtggtttt atctttcaag
     5881 ctttacttct attatctagg cttttttacc aagaaaagag ggaatataag gtttttaatc
     5941 cttatcgcta ca
//
LOCUS       DASRNA010000060         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_464450,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000060 DASRNA010000000
VERSION     DASRNA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464450"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VFD08_01735"
     CDS             <1..1003
                     /locus_tag="VFD08_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09768.1"
                     /translation="SYSKKLEQIRLEKKALEEKIYRKAEDQRNELFLQRADLVVEEEK
                     EERSIRSELSKIIAGHSETFFHNMQEIGKLDHWIAKAKLARSYPSCMPKLLEEKTLLL
                     TQASNPMIADLLRREMADFQPINLELKAGTTVITGANMGGKSVSLKTLLLNVILVHLG
                     MLPFAKEASLGLFDFIFYLGDDYEDVKSGLSSFGAEVLKLRDILKRTKKEKGLLVLDE
                     PARGTNPKEGRAIVKGLAQHFLGGENILLLATHFDQIVEPGMVHYQIRGFEGVDFNKL
                     RDNLQIQEGRSLEILRSQMDYRLELVSGGQEVPKDALRICSLLGLDRELIETINKLL"
     gene            1015..2571
                     /locus_tag="VFD08_01740"
     CDS             1015..2571
                     /locus_tag="VFD08_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HZK09769.1"
                     /translation="MSKLNLDKAKVEKARELARKIAKETQQFVDIHTTVTVERTICRL
                     LGIDGVNDIEIPLPNIVVDQLEENKALGIGAAFYLSNAMIQTGKSPQEIAEAISRGEL
                     DITKLALADPFEIKVNIQKTAEDMVDQIRKNRQERESYLSRFGDAQGAYLYVIVATGN
                     IYEDITQAKAAARQGADIIAVIRTTGQSLLDYVPYGPTTEGFGGTMATQENFRLMRAA
                     LDEVGEELGRYIRLCNYCSGLCMPEIAAMGAIERLDVMLNDALYGILFRDINMKRTMV
                     DQYFSRVINGFAGVIINTGEDNYLTTADAYEEAHTVLASQLINEQFALMAGLPEEQMG
                     LGHAFEMDPAMENAFVYELAQAQMAREIFPKAPLKYMPPTKFMTGNIFKGHIQDALFN
                     MVTVLTGQKLQLLGMMTEAIHTPFMSDRALAIENAEYIYHTMKGLEDEIQFKPGGMMQ
                     KRVAFVIDEAIELLEEMEGLGLFKSLELGKFGDVKRPQDGGKGLAGVTVKEKEYFNPF
                     IPLFLEKEVD"
     gene            2571..3350
                     /locus_tag="VFD08_01745"
     CDS             2571..3350
                     /locus_tag="VFD08_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HZK09770.1"
                     /translation="MKAELTKKTLDEKVDRKNLRPYGDTINDGKVQLSFTLPIKDDEK
                     GVEAAKELAKKMGLLDPLVSWHQALDEEFTFYIVYGSVTHGVNYEDIHVDAVEIETMT
                     MEETDAFIKEKIGRPLVIIGASTGTDAHTVGIDAIMNMKGYAGHFGLERYEMIDAYNL
                     GSQVENEEFIRHAVEKKADVLLVSQTVTQKEVHIHNLTHLVELLEAEGLRDKVILIAG
                     GPRLSNELAKELGYDAGFGPGKFADDVATYAVEEFIARDMK"
     gene            3488..>4313
                     /locus_tag="VFD08_01750"
     CDS             3488..>4313
                     /locus_tag="VFD08_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HZK09771.1"
                     /translation="MNMKTKKLYDDAYIKTHKAKVLAIKPSGALVLDETIFFPEGGGQ
                     LPDQGFIEGQPVLDVQEEGDYILHYIKSTEDFLIGQEVELELDWESRFNSMQQHTGEH
                     ILTGIAQSLYGTKNVGFHIGKDMMRVDYDKELSWEQLEKLERKANEAISQNVPIVSFY
                     PMPSVLESLPYRSKREIEGDVRVVEVKGFDICACCGTHVRLSGEVGLLKILSRENYKG
                     GVRLGVLCGFKALEYFDKLAKQAMEMSHLLCAPALELYPALEQLYEDRENIRRLSVE"
BASE COUNT         1376 a          815 c         1016 g         1106 t
ORIGIN      
        1 tagctatagc aaaaaacttg agcagattcg cctagaaaaa aaggccttag aagaaaaaat
       61 ttatcgtaaa gcggaagatc agcgaaacga actcttttta caaagagctg atcttgtagt
      121 ggaagaagaa aaagaagaac gcagcattcg cagcgaactt tctaaaatca tcgctggtca
      181 tagtgaaaca tttttccata atatgcagga aattgggaag cttgatcact ggattgccaa
      241 agccaagctt gccagaagct atccttcttg tatgccaaaa cttctagagg aaaaaacgct
      301 tctacttact caggcaagca accccatgat agcagatctt cttagaagag aaatggcaga
      361 cttccaacca ataaacctag agcttaaggc aggaacaacg gtcattaccg gagccaatat
      421 gggaggaaaa agtgttagct taaaaactct tcttcttaat gtgattttgg tgcatcttgg
      481 tatgctgccc tttgcaaaag aggcctccct tggcttgttt gattttatct tctatcttgg
      541 agatgattat gaggacgtta agtccgggct atcaagtttt ggtgcggaag tactcaaact
      601 aagagatatt ttaaaaagaa ctaaaaagga aaagggcctg cttgtactcg atgaaccagc
      661 caggggaacc aatccaaaag aaggaagagc aatcgtaaag ggacttgcac agcactttct
      721 tgggggagaa aatattctac ttcttgccac tcactttgat caaattgttg aacccggaat
      781 ggttcattat caaataagag gttttgaagg agtggatttt aataagctaa gggacaattt
      841 acagattcaa gaaggaaggt ccctagaaat ccttcgatcc cagatggact atcgcttgga
      901 gcttgttagc ggaggccagg aagtacccaa agatgccctg cgtatttgtt ccttactagg
      961 tcttgatagg gagcttattg agaccataaa taaattgtta tagggaggaa acatttgagc
     1021 aaacttaatt tagataaggc caaggtagaa aaggctagag aattggcaag aaaaattgcc
     1081 aaagaaaccc agcaatttgt agatattcat actaccgtaa cagtagaacg aacaatttgc
     1141 cgccttttag gcattgatgg ggtcaatgat attgaaatac cccttccaaa tatagtggta
     1201 gatcaactgg aagaaaacaa ggctctaggt attggagctg ctttttatct ttccaatgcc
     1261 atgattcaaa caggaaaatc accccaagaa attgccgagg ctatttcacg cggggagctt
     1321 gatattacaa agctagcttt agcagatccc tttgaaatca aagtcaatat tcaaaaaaca
     1381 gcagaagata tggtagacca gatcagaaaa aatagacaag agcgagaaag ctacttgtcg
     1441 cgctttggtg atgcccaggg agcctacctc tacgtaattg tagccacagg taatatttat
     1501 gaggatatta ctcaggccaa ggcagctgct aggcaaggag ccgatattat tgcggttatt
     1561 cgaaccacag gccagagcct actcgactac gttccctatg gaccaacaac agagggcttt
     1621 gggggcacta tggctaccca agaaaacttt cgtctaatgc gggcagcctt agatgaagtg
     1681 ggcgaagaac taggcagata tatccgcctg tgcaactatt gttcaggcct atgtatgcca
     1741 gaaattgctg ccatgggagc aattgagcgc ctggatgtta tgctaaatga tgctctttac
     1801 ggtatcttgt ttagagatat aaatatgaaa cggacaatgg ttgaccagta tttctccagg
     1861 gtaattaacg gctttgctgg agtcattatc aatacaggag aagataacta tcttacaacg
     1921 gccgatgcct atgaagaagc ccatactgta ctagccagcc agctaattaa tgagcaattt
     1981 gctctaatgg caggacttcc tgaagaacaa atgggcctag gtcacgcctt tgagatggat
     2041 ccagccatgg aaaatgcctt tgtttatgaa ctggcccagg cccagatggc aagagaaatc
     2101 ttccctaagg ccccacttaa atatatgcct cctaccaagt ttatgacagg aaatatcttt
     2161 aagggtcata tccaggacgc cctatttaat atggttactg ttttaaccgg ccaaaaactt
     2221 caacttttag gcatgatgac tgaagcaatt catactccct ttatgtcaga tagggctctg
     2281 gccattgaaa atgcagagta tatctatcat acgatgaagg gcctagaaga tgaaatccag
     2341 tttaagcccg gtggtatgat gcaaaagcgt gttgcttttg ttattgatga ggccattgag
     2401 ctactagaag aaatggaagg gctaggactc tttaaatccc tagaactagg aaagtttggc
     2461 gatgtcaaac gtcctcaaga tggaggaaag ggccttgccg gcgttacggt caaggaaaaa
     2521 gaatatttca acccctttat cccactattt cttgaaaagg aggtagacta atgaaggcag
     2581 aactgactaa gaaaactctt gatgaaaagg tagatcgtaa gaatcttcgt ccctatggag
     2641 atacgataaa tgatggaaaa gttcagctta gttttactct tcctattaag gacgatgaaa
     2701 agggtgttga agcagccaag gaactagcaa aaaaaatggg tcttctagat cccctagtat
     2761 catggcacca ggccctggat gaagagttta ccttttacat tgtatatggc tctgtgaccc
     2821 atggggtaaa ctatgaagat atccatgtgg atgcagtaga gattgaaacc atgaccatgg
     2881 aagaaaccga tgcctttatt aaggaaaaaa taggccgccc cctggttatt ataggggcta
     2941 gtactggtac tgacgcccac acagttggta ttgatgctat tatgaatatg aagggctatg
     3001 ctggtcattt tggccttgag cgctatgaaa tgattgatgc ctataatctt ggcagccagg
     3061 tagaaaacga agaatttatc cgacatgctg tagagaaaaa agcagatgtt cttctggtaa
     3121 gccaaacggt aacccaaaaa gaagttcata ttcacaattt gacccatcta gtagagcttc
     3181 ttgaggccga gggcttacga gataaggtga tccttattgc tggaggtcct cgtctaagca
     3241 atgagctggc caaggaactt ggctatgatg cgggctttgg tccaggtaaa tttgctgatg
     3301 atgtggcaac ctatgccgtg gaagaattta ttgcccggga tatgaagtag cagacaaaac
     3361 gaatagagat aaaaagagga accttcctag taaaagaagg ttcctttcct ttttagggta
     3421 gctggcctcc tttatcgggt atatagtaga ttgggattat caatgaataa aaagaaggaa
     3481 tgcaaaaatg aatatgaaaa caaaaaaact ttacgatgat gcctatataa aaacccacaa
     3541 ggccaaggta cttgctatta aaccatcagg agctctagta ctagatgaaa caattttttt
     3601 cccagaagga ggagggcaac ttcctgatca aggatttatt gagggccagc ctgttttgga
     3661 tgtgcaagaa gaaggagact atattcttca ctatatcaag tccacagagg actttcttat
     3721 aggtcaagaa gtagagcttg aacttgactg ggaaagccgc tttaacagca tgcagcagca
     3781 tacaggagag catattttga ccggtatcgc tcagagcctc tatgggacaa aaaatgtggg
     3841 ctttcatatt ggaaaagaca tgatgcgggt tgactatgat aaggaactta gctgggagca
     3901 gctagaaaaa ctagaaagaa aagccaatga agcaatcagc caaaatgtgc ccattgttag
     3961 cttttatccc atgcccagtg tcctagaatc cctaccctat cgtagtaaga gggaaattga
     4021 gggggacgtt cgggtggttg aggtaaaggg ctttgatatt tgtgcttgtt gtggtaccca
     4081 tgtaaggttg agtggggaag tgggcctact aaaaatcctt agtagggaaa actataaggg
     4141 gggagtgcgc ttgggcgtgc tttgtggctt taaagccctt gaatactttg ataaactcgc
     4201 caagcaggcc atggagatga gccaccttct ttgtgcacca gccctagaac tctatccggc
     4261 cctagaacaa ctttatgaag accgggaaaa tatacgaagg ctctcagtag aaa
//
LOCUS       DASRNA010000061         7136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_615512,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000061 DASRNA010000000
VERSION     DASRNA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615512"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VFD08_01755"
     CDS             complement(<1..1827)
                     /locus_tag="VFD08_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HZK09772.1"
                     /translation="MNLLQQKVKEVGQSLFPIIILILLTIFGLVDVSYQVLLRFLLGA
                     VFLFLGLSLFLWGVDQSMNPIGFHMASEIATSKKLSKALVLAFVLGFLINIAEPDLII
                     LGSQVQQASGGQIIGQSMVALVSIGVAFMVTFGSLRVLLNKRYNVMMAIVYGLILVLS
                     FFVADEFLAISFDASGATTGALTTPFILAISLGLSKMKGGDKSEDNAFGLVGAMSAGP
                     MPAVMLMAIFTKQSRILGEASAFVETTGVLRPVFQSIPATFMESLQALGPIALLFLFY
                     NYRSFKLNKRELIGILRGLIYSLIGLTFFLVGANQGFMEMGRVLGMGLADSQPYLLPL
                     IGFLAGLIIVTAEPAVHVLGEQIRQVSAGAIPIRTIRLTLSLGVGTAIALSMLRIMIP
                     AIKLWYFLLPGFSLAIFLSFFAEPIFVGIAYDAGGVASGPMSATFLLAFAQGAADIIP
                     TANVLVDGFGIIAMIAMTPVLSLMILGTIIAYRKKKALLPQHLEDMAEESLPQIQRDS
                     THKDLIFVLVQHGKSYETILMARTLGARGATMIHGRDARAQALSGFSLNMDPEKEVLL
                     LIVNSSITENLLANLLEEQDKNPNSGIDSLMVLPASFHRGFNS"
     gene            complement(1824..2489)
                     /locus_tag="VFD08_01760"
     CDS             complement(1824..2489)
                     /locus_tag="VFD08_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZK09773.1"
                     /translation="MLSQILFITVVDRGRAEALLKQMQAFSVGGGVVVLGEGTISNPW
                     LEILGLNESQKDIIFLPIPAGFEDSLHQLMRKEFKLHKRHRGISFSLPLSHFQRMHFR
                     ESNMRIDPKNYQYHCIICILERGKAKDCVYHAQKAGASGGTILHGRGAGVPQDSFFEL
                     LLEPQKDIVFFIVATDIVNQVQEEIATKMELEKQGQGIIFALPVNKATGLYQAEEKAG
                     ESK"
     gene            complement(2535..3404)
                     /locus_tag="VFD08_01765"
     CDS             complement(2535..3404)
                     /locus_tag="VFD08_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK09774.1"
                     /translation="MKYYGKVFRPPSEASSLIIQCTVGCSHNQCTFCGMYKEDRFRLR
                     PLEDILRDLEQLAPYRHVYRRVFLGDGDALILPMESLRAILKKIKQVFSSVERIGIYA
                     SPKSIETKTVGELKELKDLGLGIAYLGLESGSETILKKVKKGSTAEEILALSKRIKEA
                     GILLSVTLISGLGGKELTKEHAIKSAHIVSQMAPDYLGLLTLMQEPATELYRDIREGR
                     FQVLSPREVLEETRLLVSLIEGENILFRSNHASNYLALRGNLAEDKKTLLDSIDLALG
                     GKESLRAEAHRAL"
     gene            3474..4367
                     /locus_tag="VFD08_01770"
     CDS             3474..4367
                     /locus_tag="VFD08_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZK09775.1"
                     /translation="MKDIILARDKKELYYRYWPAQKEEKVLQLVHGANEHSGHYDEFA
                     RWMNKRGVSVYALDNRGHGLNPLVEGYIHINPEDWEKLPEDVILLGELIKEKTNKIPY
                     LLGHSMGSFIARIVALKGEEYKGFFFLGTGWEPQLKLQSGLALAEGLILLHGDHYQGP
                     LMERLIYEVYRREIIKKGDAKSFFGWFTQDPDKLTEAEEKAALIEGFSLGAFKSLLHL
                     VEAAQETENISKITAPVHFLSGEKDPVGKSTRAVDQAYRAYKEAPFGAYRHIYPQMYH
                     EILNDEAREEVYRDLLYLMQI"
     gene            4450..5703
                     /locus_tag="VFD08_01775"
     CDS             4450..5703
                     /locus_tag="VFD08_01775"
                     /inference="COORDINATES: protein motif:HMM:NF039199.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HZK09776.1"
                     /translation="MKERGSITVFFSLLLPFFLIILLLLSELSYYHFLVQREISKNYL
                     EIDHMLSGYNRELFNEMGLFAFKGRKGLAPLSDREILEESILLLMKEAHLRDGIYLAE
                     NLSSELLKTKVGIDFELFDLGGLNRELSSIVSRAKRGELSESIRIEFFAKALAMQPYI
                     HIRGISLYQLEKYISEGNIKALEEINPIFVLDDGIRDNYYKWQKSVKKFDLLNLLGSY
                     ALADYGVDYLGYSMTKKEREDLRSEYILTGLAPGSSQENMVKAELFSLRLLLNLIECF
                     SNPTVRDRILSLSGGEPTLFTVFALAQAGLESKLDVKQILKREKVPLYKGREGFRTLG
                     KVGPYQKGWTYPEYLKILLGLVSKEVYFSRLSLAIEENYDILLEFSFTGMQRKKLLSF
                     KGNYLPFEIEKEIEGRLYYVPLPEG"
     gene            5681..6109
                     /locus_tag="VFD08_01780"
     CDS             5681..6109
                     /locus_tag="VFD08_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09777.1"
                     /translation="MYPYQKGSLSLEAAFVISVFLVLLMLFYGMIYGYRAQKLAFEAA
                     TIFVLEKNTEVLLEKDFEEKLTSSLISVEKGYIFKTMKRFEEDFFYKENGFSHPLEEV
                     VFITDYGKMYHLPGCPTVKKSLRPVMKKDVPHLSPCSVCH"
     gene            complement(6132..7007)
                     /locus_tag="VFD08_01785"
     CDS             complement(6132..7007)
                     /locus_tag="VFD08_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZK09778.1"
                     /translation="MRIHIIHNLKAGRSNTNMGEELALALLQEGHRVSYHPTGGMMDA
                     ANEAQKQCELQEVDLLLAVGGDGTLNEVINGIMRSEKKLPLAPFAGGTSNDFCSYLKL
                     PESPRAYARFLIKEAKANTIDIGKVEDRYFINVVACGMLSEVAHNTDYQLKSVLGSFA
                     YYMEGLKEIVTRGLYNNIEIKSEGYHYKGEYVAFLVSNSHTIGGFKNLSPDASIHDGY
                     LNALLIKKAPFVNLLDIAIKLDRGTHINNRYVDYFTTKDLCVETDSEMDVDGEHLQYQ
                     KANIEAIPCAIVCYY"
BASE COUNT         2126 a         1404 c         1574 g         2032 t
ORIGIN      
        1 gctattaaag cccctatgga aggaggctgg caggaccata agagagtcaa tgccgctatt
       61 tgggttttta tcttgttctt ctaacaggtt ggccaaaaga ttttctgtga tcgaagagtt
      121 tacaataaga agaagcactt ctttttcggg gtccatattt aggctaaagc ccgaaagagc
      181 ttgtgcccta gcatctcggc catggatcat ggttgcccct cttgccccca aagttcttgc
      241 cataagaatg gtttcgtagg attttccatg ttgaacaagg acaaagataa ggtctttatg
      301 agtgctgtct ctttgaattt gtggcaagct ttcctctgcc atatcttcaa ggtgttgagg
      361 taggagggct ttcttttttc ggtaggcaat aatggttccc agaatcataa ggcttagaac
      421 cggagtcatg gcaatcatgg caataatacc aaaaccatct acaaggacat tggcggtggg
      481 gataatatca gctgccccct gggcaaaagc cagaagaaag gtggcgctca tgggccctga
      541 tgcaacccct ccagcatcat aggcaatgcc aacaaaaatc ggttcggcaa aaaaagataa
      601 gaaaatagcc aaggaaaaac caggaagtaa aaaataccaa agtttaatag cgggtatcat
      661 aatccgcagc atggaaaggg caatggctgt accaacccca agagataggg taagtcggat
      721 tgttctaatg ggaatagcac ctgcactgac ctgtctaatc tgttcgccta gaacatggac
      781 agcgggctcg gctgtgacaa taataaggcc agcgagaaat ccaataagag gcagcaggta
      841 gggctggcta tcagcaaggc ccattcctaa gacccggccc atttccataa agccttgatt
      901 ggcaccaaca agaaaaaagg taagaccgat aagcgaatag atcaggcccc gcaaaatacc
      961 aataagttct cttttattta gtttgaaact tcgataattg tagaataaaa atagcagggc
     1021 aatggggcct aaggcttgta aagactccat aaaagtagca ggaatgcttt gaaaaacagg
     1081 tcgtaagaca ccagtagttt caacaaaggc actagcttct cctaggatgc ggctttgttt
     1141 tgtaaaaata gccatcagca taacagctgg cataggacca gcactcatgg cacctacaag
     1201 gccaaaggca ttgtcttctg atttatctcc ccccttcatt tttgaaagac cgagggagat
     1261 ggctaggata aagggagtag taagagctcc tgtggtggcc ccagaggcat caaaggaaat
     1321 ggccagaaac tcatcggcta caaaaaaact taggacaagt ataaggccat agacaattgc
     1381 catcataaca ttatatcttt tattgagcag gactcgaaga gaaccaaagg taaccataaa
     1441 agcaacccca atagaaacaa gggctaccat ggactggcct ataatttgtc cccctgaagc
     1501 ctgttgcact tgagaaccta ggataattag atcgggttca gcaatattga ttagaaagcc
     1561 cagaacaaag gcaaggacca gggccttgct taattttttt gaggtggcaa tttccgatgc
     1621 catatgaaaa ccaatagggt tcatggattg gtctactccc cataaaaaaa gagaaaggcc
     1681 aagaaagagg aaaacagccc ccagaagaaa acgcagtaga acctggtagc tgacatcaac
     1741 aaggccaaat atagtaagta gaattaaaat aataatagga aaaagggatt gtcctacttc
     1801 ttttaccttt tgttgtagta gattcatttg ctctcaccgg ctttttcttc cgcctggtaa
     1861 agaccagtgg ctttatttac aggtagggcg aagataatcc cttgcccctg tttttccagc
     1921 tccatcttgg tggcgatttc ttcttgcact tgatttacaa tatctgtagc tacaataaaa
     1981 aagacaatat ctttttgtgg ctcaagtaaa agctcaaaaa aactgtcttg gggcacaccg
     2041 gcccctcgcc catggaggat ggttcctcct gatgcgccag ccttttgggc atgataaaca
     2101 cagtccttgg ccttgccgcg ctcaaggatg cagataatac agtgatattg gtaattcttt
     2161 gggtcaatgc gcatattgct ctccctaaaa tgcattcttt ggaaatgact aaggggaagg
     2221 gaaaaggata tgcctcgatg gcgtttatgg agtttaaatt ccttgcgcat tagctggtga
     2281 agggaatctt caaagccagc gggaatgggc aagaaaataa tatctttttg ggactcattt
     2341 agtcctaaaa tttcaagcca agggttggag atggtccctt ctcctaggac tacaactcct
     2401 ccccctacac taaaggcttg catttgttta agtagagctt cagcgcggcc cctatcgacc
     2461 accgtaataa aaagtatttg tgataacata agcgaacctt tctccagatg gttccataga
     2521 agaaaaaact cgaattataa ggccctgtga gcctcggccc ggaggctttc tttccctcct
     2581 aaggcaaggt caatagaatc cagcagtgtt ttcttatcct ctgctagatt gccccgaagg
     2641 gccagatagt ttgaggcatg gttggagcga aagagaatat tttctccctc aattaagcta
     2701 acaagaagcc gggtttcttc caggacctcc ctgggggaaa gaacttgaaa tcttccttct
     2761 ctaatatccc tataaagttc tgttgctggc tcttgcatca gggtaagaag gcccagataa
     2821 tcaggagcca tctggcttac aatatgggca ctcttgatgg catgttcttt tgtcagctcc
     2881 ttgcctccaa gaccggaaat aagggtaaca gaaagaagga ttcctgcttc ctttattctt
     2941 tttgaaagag caaggatttc ttcagcagtg gaaccttttt tcactttttt taggatggtt
     3001 tcacttccgc tttctagtcc aagataggca atgcctaggc ctagatcttt tagctctttt
     3061 aactctccaa cggtttttgt ctctatactc tttggcgagg catagatgcc aatgcgctca
     3121 acagaagaaa aaacctgttt gatttttttt aggatggctc gtagactttc cataggaagg
     3181 attagggcat ccccatcccc taagaatact ctgcgataaa catggcgata gggcgccaat
     3241 tgctcaaggt cccttaaaat atcctctagg ggacgtagtc taaaacgatc ttctttatac
     3301 attccgcaaa aggtgcactg gttatgcgag caaccaacgg tgcactgaat aatcagactt
     3361 gaagcttctg atggcggacg aaaaacttta ccatagtatt tcatggtaac ctccatttct
     3421 ttttgggtat caatacatta attatatcag actttcatag caaggagata aatatgaaag
     3481 atattatact agcaagagat aaaaaagaat tatattaccg ctattggccc gcacaaaaag
     3541 aagaaaaagt cttgcaactt gtccatgggg ccaatgaaca ttcggggcac tatgatgaat
     3601 ttgcccgctg gatgaataag agaggggtta gtgtctatgc tcttgataat agaggccatg
     3661 gcttaaatcc tctagtagaa ggttatatac atattaatcc tgaggactgg gagaaacttc
     3721 ctgaggatgt tattttatta ggagaactaa ttaaagaaaa aacaaataag ataccctatc
     3781 ttctgggcca tagtatgggt tcttttattg cacgaattgt ggccttaaag ggagaagagt
     3841 ataagggttt tttctttctt ggaacaggtt gggagcccca gctaaaatta cagtccggtc
     3901 ttgcccttgc tgagggcctt attcttcttc atggagacca ctatcaaggt cccttaatgg
     3961 aaagattgat ctatgaggtg tatcgacgtg aaataattaa aaaaggagat gcaaagtctt
     4021 tttttggttg gtttactcag gatccagata agctaacaga agccgaagag aaggctgccc
     4081 taatagaagg cttttctctc ggagccttta aaagcctgct tcaccttgta gaggctgccc
     4141 aggagacaga aaatatttct aaaattacag ctccagtgca ttttttaagc ggagaaaagg
     4201 accccgtagg taaatctacc agggcagtgg accaagccta tagggcctat aaagaggcgc
     4261 cctttggagc ctatcgccat atttatccgc aaatgtatca tgaaatctta aatgacgaag
     4321 caagagaaga agtctatagg gatttgcttt atcttatgca aatctaaaaa gtatgcctat
     4381 atgtaaaaaa gaagagattt gattctcttc tttttttatt ggcgtaaact aatcctaggg
     4441 ggtgcacata tgaaagagcg aggatcaata acggtttttt ttagccttct tcttccattt
     4501 tttcttatta ttttgctgct tctttctgag ttaagttatt atcattttct tgtgcaaagg
     4561 gaaatcagca agaactatct tgaaattgac catatgctat caggatataa tcgggagctc
     4621 tttaatgaga tgggtctttt tgcctttaaa ggaagaaagg gactagctcc cttatccgat
     4681 agggagattt tggaggagag tatccttctt cttatgaaag aagcccatct tcgcgatggt
     4741 atttatttgg cagaaaacct tagctcggaa ctcctaaaga ccaaggtggg tattgatttt
     4801 gaattgtttg atcttggggg acttaatagg gagctttctt ctattgtatc gagggcaaaa
     4861 agaggagagc tttctgaatc catacgaatt gagttttttg ccaaagccct ggccatgcag
     4921 ccctatattc atattcgagg aatcagccta tatcaattgg aaaaatatat ttctgaggga
     4981 aatattaagg ccctggaaga aataaaccct atctttgttt tagatgacgg gattcgagat
     5041 aattactata agtggcaaaa atccgtaaag aagtttgacc ttcttaatct tctaggtagt
     5101 tatgctctgg ctgattatgg ggtggattat ctgggctact ctatgacaaa aaaagaaaga
     5161 gaagacctgc gcagtgagta tattctgaca ggccttgctc ctggaagtag ccaagaaaat
     5221 atggtaaagg cagaactttt tagtctacgt cttcttctta acttaataga atgttttagc
     5281 aatccaacag ttagagacag gatactttca cttagtggag gagaaccaac cttatttact
     5341 gtttttgccc tggcccaggc cggtcttgag agcaagcttg atgtcaaaca aatactcaaa
     5401 agggaaaaag ttcccctcta taagggaagg gagggtttta ggacgctggg aaaagtgggc
     5461 ccctatcaaa agggttggac ctatcctgaa tatcttaaga ttttgctggg acttgtttcc
     5521 aaggaagttt atttttcgcg gcttagccta gccatagaag aaaattatga tattttactg
     5581 gaattttctt ttaccggtat gcaaagaaaa aaactgctga gctttaaagg caactatctt
     5641 ccctttgaaa tagaaaaaga aattgaaggg aggctttact atgtacccct accagaaggg
     5701 tagtctaagt ttagaggcgg cttttgttat ttctgttttc cttgtattac ttatgctttt
     5761 ttatgggatg atttatggtt atagggccca aaaactggcc tttgaggcag caaccatttt
     5821 tgttttagaa aagaacacag aagttctgct tgaaaaagat tttgaggaaa aactgactag
     5881 ctcccttatt tctgtagaaa agggctatat atttaagacc atgaagcgct ttgaggaaga
     5941 ctttttttat aaagaaaatg gcttttctca ccccttagaa gaggttgtct ttattactga
     6001 ttatgggaag atgtatcacc ttcctggctg ccctacggta aaaaagagtc ttcgacctgt
     6061 aatgaaaaaa gatgttccac atcttagccc ttgcagtgtt tgtcattaaa aaaagccact
     6121 ctgggtggcc tttagtagta gcagacaatc gcacagggga tagcctcaat atttgctttt
     6181 tgatattgaa ggtgctcgcc atcgacatcc atttcactat ctgtttcaac acataggtcc
     6241 tttgtggtga agtagtcaac atagcgatta tttatatgtg tccctcggtc aagctttatg
     6301 gcaatgtcta aaaggttgac aaagggcgct ttttttatta gaagggcatt gagataacca
     6361 tcatgtatac tggcatcggg ggaaagattt ttaaaaccac caatggtgtg ggaattgcta
     6421 actaaaaaag caacatactc tcccttataa tgatagccct cacttttaat ttcaatatta
     6481 ttatagagcc ctcgagtgac aatttctttt agtccttcca tataataggc aaaggagcca
     6541 agaacgctct taagctgata gtctgtatta tgggctacct ctgatagcat gccacaggcc
     6601 acgacattga taaagtaccg atcttccact ttaccgatat ctattgtgtt ggccttggct
     6661 tcctttataa gaaagcgggc atatgccctt ggagattcgg gtagttttaa ataggaacaa
     6721 aaatcattgg atgttccacc ggcaaaaggc gccaggggga gttttttctc tgaacgcata
     6781 attccattga ttacttcatt tagtgttcca tctcctccaa cagccaggag taaatccacc
     6841 tcttgtagct cacattgttt ttgcgcttca ttggcggcgt ccatcatccc acctgtagga
     6901 tggtaggaaa ccctatggcc ctcttgaaga agggccaagg ccaactcttc gcccatattg
     6961 gtatttgatc ttccagcttt aagattatga atgatatgta ttcgcataaa tcctccttgt
     7021 gtcattatac tctaaattga ggggggatga taggcagaag tctatctttt caagggggta
     7081 tagatggggt ataatcaaat acatagaaag aggaggtatg atgaaagagt taatag
//
LOCUS       DASRNA010000062         5512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1063628,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000062 DASRNA010000000
VERSION     DASRNA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063628"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..602)
                     /locus_tag="VFD08_01790"
     CDS             complement(87..602)
                     /locus_tag="VFD08_01790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK09779.1"
                     /translation="MRSALDFVHQLIPENIDLAIDATVGRGRDSLILASRSKRLIGFE
                     IQKEALEEAKLLLKDYNVRLYKDCHSQMDLYVKEKVDLIIFNLGYLPRGDKRICTRAE
                     TTQLAIKKGLELLHTGGRILIVAYKHPKGQDEIKMLENLDLPQNQADIFRLTHYNGKN
                     HPPEAWVIIKK"
     gene            complement(586..1281)
                     /locus_tag="VFD08_01795"
     CDS             complement(586..1281)
                     /locus_tag="VFD08_01795"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZK09780.1"
                     /translation="MRLQQFMARAGVASRRKSEDIIRQGRVHVNGVLAKIGQKVSPED
                     EVLLNGKALMGAESFVYFAFNKPKKVLSTVSDSRGRKTVIDFYKGKKRIFPVGRLDYD
                     SHGLLLCTNDGDFAQLFIHPRYGFDKDYILTLNTELTTKEIRSLEMGVYLDGYKTRPI
                     TIKKIGEKQYLFTLVEGRNRQLRRMVEIKGKTVLDLRRIRVGPFSLGDLPEAEFLPLK
                     IKEVERLKHALRT"
     gene            complement(1278..1784)
                     /gene="scpB"
                     /locus_tag="VFD08_01800"
     CDS             complement(1278..1784)
                     /gene="scpB"
                     /locus_tag="VFD08_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZK09781.1"
                     /translation="MEDLRKLELLLFVAREPLSTRRAASLLKLTPSTTEKLIEELAQE
                     YENQKKAITIQSFGNFHQLASNGRYDDLIEAFLGSSPNRGLGPSTLEVLSIIAYRQPV
                     TRVQVDELRGVSSDYSIRVLLERDLISVSGTLDTLGNPKLYITTPRFLRNFNLKNLDE
                     LPTIGDQA"
     gene            complement(1771..2499)
                     /locus_tag="VFD08_01805"
     CDS             complement(1771..2499)
                     /locus_tag="VFD08_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZK09782.1"
                     /translation="MELNISLEEFTGPLDLILELIKKDELDIYNIPINQITSSYLEEM
                     KSRTIPSEEIAEFLRMAAYLLEIKSQMLLPDHQLESAFSDDTDPRYNLIHRLLEYQKV
                     KEATLFLMTREAPLKIYSDDLPFILPKPKPASLVFDLDSSVLKKAMEDVLNRHDYNNY
                     SLLYDKLKKDSYSVFLEQKEILTYLSYKMKLNFTSLLMDAPKAKQVASFLALLKLAQD
                     QRILVHQEENFSDIEIERRSHGRS"
     gene            complement(2490..3812)
                     /locus_tag="VFD08_01810"
     CDS             complement(2490..3812)
                     /locus_tag="VFD08_01810"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZK09783.1"
                     /translation="MRMYDVIYKKRKGEKLTEEEIAFFISSYVSGDTPDYQASAFLMA
                     CFLQDLDEEETYFLTRQMLNSGHKMDLSALEGIKVDKHSTGGVGDKTSLTLAPLIASL
                     GLIMAKMSGRGLGHTGGTIDKLESIPGFVTELREEDFIQQSNDIGLALISQTKNLVPA
                     DKLLYALRDVTATVDHPALIASSIMSKKLAAGTDYILLDVKVGSGAFLKNIDEAIHLA
                     KIMVSIGERAGKKTRAIISSMDQPLGYKIGNSIEVEEAIELLQDKGPQDLKELTLVLS
                     SQLLIMAGKCKNMDKAYEVSRKHLESGYGYDKFLELVKAQGGKSLNFVQPLFERKVYA
                     PSSGYIESFDTQAIGESASILGAGRKTKDEAIDLTAGIYLPFKIGDYIKKGDLLAKLY
                     SSRKNVFTDSEAKLLSSIVLNQNKVKSHKLIRALVYEESGQSKVEMWS"
     gene            complement(3799..4959)
                     /locus_tag="VFD08_01815"
     CDS             complement(3799..4959)
                     /locus_tag="VFD08_01815"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HZK09784.1"
                     /translation="MKRVIIIILDSLGIGNAPDAEAFGDLGANTLKSVMSENPDIRNL
                     RKLGLGNINEVDYIEDISDPLALYGSLEEISAGKDTTIGHWEIAGLEIHTPLPTYPNG
                     FPKEIMDEFEEKIGRGTLCNLPYSGTEVIKEYGPEHIKTGKPIVYTSNDSVFQIAAHE
                     DVIPLKELYAMCEIARKILRGDHALARVIARPFIGENGNYTRTSNRRDFSLSPFEPTL
                     LDHLKQQGFDVISVGKIYDIFNGSGLTQEQHTDSNMDGVNWILKKMDTNFSGLLFANL
                     VDFDAQFGHRRDPKGYAKALEEFDQRLPEILSKLRKDDLLIMTADHGNDPCFKGTDHT
                     RERVPVLFYKKGINPRNIGSRRGFHTIAATIYDYLGAQGSKRGESVLKHENV"
     gene            complement(4961..>5512)
                     /locus_tag="VFD08_01820"
     CDS             complement(4961..>5512)
                     /locus_tag="VFD08_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZK09785.1"
                     /translation="AEKSFAKMAKGLKEVLQFLELDEAEFSLAIDEKNPEEIYLDGHY
                     RLSFLASMNKGPLRPMANILSGGEMSRLMLALKSILSTDDETMIFDEIDAGISGRVAH
                     QVGKQIHHLSKSRQILAISHLPQVVAFSDEHFFLEKKDGRTSVKKLNQKEHEEVLATM
                     MSAELNKASLQTAKNMIFRAKGE"
BASE COUNT         1573 a         1109 c          988 g         1842 t
ORIGIN      
        1 atatacagtg tatcatgtct agtaataaat gcaataaaaa atgcaacatt tcataatact
       61 cgcaattatg aaagctgcat tgcatatcat tttttaataa taacccaggc ctcgggagga
      121 tgatttttgc cattgtaatg agtgaggcgg aaaatatctg cctgattttg gggtaaatcc
      181 aaattttcca acattttaat ctcgtcctga cctttaggat gtttataggc tacgatcaat
      241 atccgtccac cagtatgtaa gagttctaag ccctttttaa tagcaagctg ggtggtttct
      301 gctctagtac atatcctttt atcaccacgg ggcaaatagc caagattaaa gatgataaga
      361 tctacttttt cctttacata gagatccatt tggctatgac agtccttgta gaggcgtaca
      421 ttataatctt taagaaggag tttggcctct tcaagtgcct ctttttgaat ttcaaatcca
      481 attagcctct tagagcgact agctaagatt agactatccc taccccggcc aactgttgca
      541 tcaatagcta gatctatatt ttcaggaata agctgatgaa caaaatcaag tgcggagcgc
      601 atgctttaac ctttctactt ctttaatttt aaggggaaga aattctgctt ctggaagatc
      661 tcccaggcta aagggtccca ctctaattct acgtagatct agaacagttt taccttttat
      721 ttctaccatg cgtcttaatt ggcggtttct tccttcaacc agagtaaaga gatattgttt
      781 ttctcctatt ttttttattg tgatgggtct tgttttatag ccatcaagat aaacgcccat
      841 ttcaagactt cttatttcct ttgttgtaag ctcagtattt agggtcagaa tataatcttt
      901 atcaaagcca tagcgtggat gaataaagag ttgtgcaaaa tcaccatcat tggtgcaaag
      961 aagtagccca tggctatcat agtccagtct tcccacagga aaaattcttt tttttccctt
     1021 ataaaaatca ataacagttt ttcttcctct agaatcagaa acggtggaaa gaactttttt
     1081 gggtttatta aaagcaaagt atacaaaact ttcagccccc attagggcct tgccatttag
     1141 tagaacttcg tcttctggac tgaccttttg tcctattttt gccaaaaccc cattgacatg
     1201 gactctgccc tgcctgatta tatcttcgct ttttcggcga gaggcgacac cagccctggc
     1261 cataaattgt tgcaatctca tgcttggtct cctatcgttg gcaattcatc gagattttta
     1321 agattaaaat tccttaaaaa tcttggggtt gtaatataaa gtttgggatt tcctagggta
     1381 tcaagggtcc cactaacact gattagatct cgctctagaa ggactcgaat ggaataatct
     1441 gatgagaccc cccgtaattc atccacttgc actcttgtta ctggttgtcg ataggcaata
     1501 attgataaaa cctccagagt tgatggtccc agtcccctat taggagagct tcctagaaaa
     1561 gcttcgatca aatcatcata tcgtccattg ctagctagtt ggtgaaaatt accaaaagac
     1621 tggatggtaa ttgccttttt ctgattttca tattcttggg caagttcctc aataagtttt
     1681 tcagtagttg aaggggttag ctttaaaaga gaagctgctc ttcttgttga aagaggttct
     1741 cttgctacaa ataacaaaag ctccaatttt ctaagatctt ccatgacttc tcctttcgat
     1801 ttctatatca ctaaagttct cttcttgatg cacaagtatt ctctggtctt gggctaattt
     1861 taaaagagca aggaaactag ctacttgttt agcctttgga gcatccataa gcaagcttgt
     1921 aaaattcaat ttcattttat aactcaaata ggtgagtatt tctttttgct ctaaaaacac
     1981 tgaataactg tcctttttta acttatcata aagcaggctg taattattat agtcatggcg
     2041 gtttaaaaca tcctccatgg cctttttaag gaccgaacta tccaaatcaa aaacaaggct
     2101 tgctggttta ggttttggta gaataaaggg caaatcatca ctgtaaattt ttaggggggc
     2161 ttctcttgtc ataagaaaaa gagttgcttc ttttaccttt tggtattcta gtagtcgatg
     2221 tataagatta taacgtggat ctgtatcatc actaaaggca gattctaact ggtggtctgg
     2281 aaggagcatc tggcttttaa tttccaaaag ataagccgcc attcgtaaaa attcagctat
     2341 ttcctccgat ggaatcgttc tacttttcat ttcctcaaga taagaggagg tgatttgatt
     2401 aataggaata ttatagatat ctaattcatc ctttttaatc agttccaata taagatcaag
     2461 gggtccagta aattcttcta gcgatatatt tagctccaca tttccacctt agattgtcca
     2521 ctttcttcat atacaagagc ccggattaat ttgtggctct taaccttgtt ttggtttaaa
     2581 acaattgaag aaagaagttt tgcctcagag tctgtaaaaa cattctttct actagaatat
     2641 agttttgcca gaaggtctcc ttttttgata taatcaccga ttttaaaggg caaataaatt
     2701 ccagcggtaa gatcaatggc ttcatccttt gtttttcttc ctgcgccaag aatagaagcg
     2761 ctctcaccta ttgcttgagt atcaaagctt tcaatatagc cagaagatgg tgcgtaaact
     2821 tttctttcaa ataaaggctg gacaaagtta agggacttgc ctccttgggc ttttacgagc
     2881 tcaaggaact tatcatatcc ataaccactt tctagatgtt ttcttgacac ctcataggct
     2941 ttatccatat tcttacactt tccagccata attaaaagtt gagaagaaag aactagtgtt
     3001 aattccttta ggtcttgagg tcccttgtct tgtaatagct caatggcttc ttctacttca
     3061 atactatttc caatcttata gcccaggggt tgatccatgg agcttataat ggctctggtt
     3121 tttttgcctg ccctctcacc aatagagacc ataattttag caaggtggat tgcttcatct
     3181 atatttttta aaaaggctcc gcttcctacc tttacatcta aaaggatata gtcagttcca
     3241 gcagcgagtt ttttgctcat aatactggag gcaattaggg ctggatgatc tacagtagca
     3301 gttacatctc gaagggcata aagaagcttg tctgcaggga caaggttttt tgtctgagaa
     3361 ataagtgcta agcctatgtc attgctttgt tgtataaaat cttcttccct aagttcagta
     3421 acaaatccag gtatagattc cagcttatca atggtgcccc ctgtatgacc tagtccacgg
     3481 ccactcattt tggccataat aagacctaga ctagctataa ggggtgctag ggtcaagctt
     3541 gttttatctc ctaccccacc ggtagaatgc ttatcgactt ttattccctc aagggcagaa
     3601 agatccatct tatgacctga gtttaacatc tgccttgtca ggaagtaggt ttcctcctca
     3661 tctaaatcct gtaaaaaaca ggccataaga aaggctgaag cctgataatc aggagtatct
     3721 ccagaaacat aagatgaaat aaaaaaggca atttcttctt ctgtgagctt ttctcctttt
     3781 ctttttttat aaatcacatc atacattctc atgctttaac acactttctc cgcgttttga
     3841 gccttgagca cctagatagt catagatggt agctgctatg gtatgaaaac cccttctact
     3901 tccaatattt ctcgggttaa ttcctttttt ataaaagaga acgggtactc tttctcttgt
     3961 gtgatctgtt cccttaaagc atggatcatt gccatggtca gcagtcatta ttagaaggtc
     4021 gtctttacgc agcttagaga gaatttcagg tagacgctga tcaaattcct ccagggcctt
     4081 ggcatagcct ttaggatctc gcctatggcc aaattgcgca tcgaaatcta caagattagc
     4141 aaagagaagc ccggaaaaat tagtatccat tttcttaagg atccaattta ccccatccat
     4201 attgctatca gtatgttgtt cttgtgttaa tccagaccca ttaaaaatat cgtatatctt
     4261 cccaacactg attacatcaa agccttgctg ctttaaatga tcaagaaggg ttggttcaaa
     4321 aggagataag gagaaatccc tacgattaga ggttcttgtg taattcccat tttcgcctat
     4381 aaagggacga gcaatcacgc gtgctagagc atgatctcct ctgaggattt ttctggctat
     4441 ctcacacata gcgtataatt cctttaaggg tataacatct tcatgagcag caatttgaaa
     4501 gacactatca ttagaagtat aaacaatggg ttttcctgtt tttatatgtt cagggccata
     4561 ttctttgatt acctcagtgc cactgtaggg caagttgcat aaggtccccc tgcctatctt
     4621 ctcttcaaat tcatccataa tttctttagg aaaaccattg ggataggttg gcaaaggagt
     4681 atgaatttct agacctgcaa tttcccagtg gccaatggta gtatctttac ctgcagaaat
     4741 ttcttctaaa gaaccgtata aagctagggg atcagatata tcttctatat aatcaacttc
     4801 atttatattg ccaaggccaa gctttctaag atttcttata tccggatttt cagacataac
     4861 agattttaag gtattggctc ctagatcacc aaaagcctcg gcatcagggg catttccaat
     4921 acccaatgaa tctaagataa ttataattac tcgtttcatt ttactctcct ttagcgcgaa
     4981 aaatcatatt tttggctgtt tgaagacttg ccttattaag ttcagcgctc atcattgttg
     5041 caaggacttc ctcatgttct ttttgattta gtttttttac tgatgttctt ccatcctttt
     5101 tctctaaaaa gaaatgttca tcactaaaag ccacaacttg aggcagatgg ctgatggcaa
     5161 ggatttgtcg tgattttgat aggtggtgaa tttgttttcc cacctgatgg gctaccctac
     5221 cagatatgcc tgcatctatt tcatcaaaaa tcatagtttc atcatccgtt gaaagaatgc
     5281 tctttagagc caacattaaa cgactcattt ctccgccaga aagaatattt gccattgggc
     5341 gaagggggcc tttattcatg gaagcaagaa aacttagcct ataatggcca tcaagataaa
     5401 tttcttctgg atttttttca tcaatagcca gggagaattc tgcttcatct aattcaagaa
     5461 attgtaaaac ttcttttaaa cctttagcca tttttgcaaa ggatttctca gc
//
LOCUS       DASRNA010000063         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_319785,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000063 DASRNA010000000
VERSION     DASRNA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319785"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFD08_01825"
     CDS             complement(<1..531)
                     /locus_tag="VFD08_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZK09786.1"
                     /translation="MKLLLLKYGEIGLKGKNRYFFESQLIENIKARLKDYQVEIISET
                     GRIYVNYEDDLVPLIVKDTFGLVQVCLCEKVPLDLEALEEKIGQMLATYELAGMTFKI
                     EARRTNKKYPMNSPELNSHLGSFVLKKFPQLRVDVHNPDFLIEVEVRNSFYVYFECHR
                     ALGGMPYGSSAKAVLLM"
     gene            complement(528..1628)
                     /locus_tag="VFD08_01830"
     CDS             complement(528..1628)
                     /locus_tag="VFD08_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZK09787.1"
                     /translation="MYFDHAATSPPSEKLNRSLYELSSLSGYNPSSSHKLGLEQRKRL
                     ENVRTQIARLIGARPEEIYFTSGATESNNLALQGFCYKKKGHIISSAVEHDSVEKNLD
                     FLESQGFNVTRLSPKNLTSKQKILEHLRDDTILVSLMSVQNETGEHYGLDGLGEALRK
                     KNIVYHRDCVQTFLKLPLDVNKEQVDMACFSSHKVGGLKGLGILYCRKGLSIEPLFFG
                     GGQESNLRPGTENTLAILALGHILKEEKMQENYNYIKKLNSYLGRGFVDLGGRLISPP
                     DASPYILAVSFPVPSEVLSTALSLKGVYVSAGSACHARSGKEARIAPYLASQELARGM
                     LRFSFSVENTHKEAETMLEILSQSLKELKKYL"
     gene            1690..2685
                     /locus_tag="VFD08_01835"
     CDS             1690..2685
                     /locus_tag="VFD08_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HZK09788.1"
                     /translation="MSKILIIFTGGTISMSIDKNLGAAVPNLSSDDILGMIKNLDEFS
                     EIEVLNYGEFPGPQISPEKMMELSMLIQENIKREDIQGVILTHGTDTLEETAFFLDLN
                     LKTTKPVVIVGAMRNASELGYDGPSNLAAAIVTVKSPESADQGVLVVLNNEVNAAREV
                     TKTNTLSLDTFKSTEFGPLGIVDNNEVLFFRKRVHYSQILVDHFEKEVYLLKSYAGMD
                     RSLIDFLVKKGARGIVIEAMGRGNLPPLVVKAIKDAIKQDVIVVIVSRCPSGRVLGTY
                     GYEGGGKFIKDLGAFFGTNLNGQKTRIKLMLALAYTQEYSQLKKIMESDSYICIS"
     gene            2673..3503
                     /locus_tag="VFD08_01840"
     CDS             2673..3503
                     /locus_tag="VFD08_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HZK09789.1"
                     /translation="MYLLEEHIDLNMDLPQGFVFDIETTGLSPSFNRVVMITYAYQDK
                     KQWVLRQEMAEEDQDESLLLLNFCQLSKNFLYAVHYNGQSFDLPFINKRLEYLALAQP
                     FDKRRGFDLYRYLRKNDHKGSLKLFDQEKKAGFFRKDTLTGREWVHLFNQFKKDKEKA
                     QREDLLLHNKEDVLGTLAILKKEKAFQDQLQARLYKSVLILDAYPGKDEIRLLLYDGD
                     VSTLDIPALSYDNLLFSIDYCNDWSAQEKRKALLAYDEKCFFENIKRELQRIKRNCPK
                     "
BASE COUNT         1106 a          701 c          684 g         1091 t
ORIGIN      
        1 cataagaagc actgcttttg ctgaacttcc atagggcatc ccaccaagag cacggtggca
       61 ctcaaaatat acataaaaac tatttcttac ttcaacttct atcaaaaaat caggattatg
      121 tacatctact cgaagttggg gaaatttctt taatacaaaa gatccaagat gactatttag
      181 ctcaggcgaa ttcataggat atttcttatt ggtccgccgc gcctctattt taaatgtcat
      241 gcctgctaat tcgtaagttg ccagcatctg ccctattttt tcttctaggg cctccaaatc
      301 aagagggact ttttcacaaa gacaaacttg cacaaggcca aaggtgtctt tgactatcaa
      361 gggaaccaga tcatcttcat aatttacata gattcttcct gtttcagata ttatttctac
      421 ttggtaatcc tttaggcggg cctttatatt ttcaatcaac tgactttcaa agaaataacg
      481 attttttcct ttaagaccaa tctctccata ttttaataat aaaagtttca taggtatttt
      541 ttcaactcct ttaagctttg agaaaggatt tctagcatag tctcagcttc tttatgggtg
      601 ttctcaacag aaaaagaaaa gcgcagcatt cccctagcaa gctcctgaga cgcaagataa
      661 ggcgctatgc gtgcttcctt gccgcttcta gcgtggcagg cgcttcctgc tgatacatag
      721 acacccttta gggaaagggc tgtggaaagg acttctgagg gcacaggaaa gcttacagca
      781 agaatataag ggctagcatc tggaggcgaa ataagccttc ctcctaggtc tacaaagcct
      841 ctacctagat agctatttag ctttttgatg tagttgtagt tttcttgcat tttttcttct
      901 tttaggatat gacccagggc caaaatagct agggtatttt ccgttcctgg tcgaagattt
      961 gattcttgtc ctcctccaaa aaacaagggc tcaatagaaa gaccctttct acaataaaga
     1021 atgccaagac cctttagccc tcccacttta tgggagctaa aacacgccat atcaacctgc
     1081 tccttgttta catcaagggg taattttaaa aaagtctgaa cacagtctct atgataaaca
     1141 atattttttt tccttagagc ctctcccaat ccatccaagc catagtgctc ccctgtttca
     1201 ttttggacac tcataagact tacaagaatc gtatcatctc ttagatgttc taagatcttt
     1261 tgcttacttg taagattttt tggtgaaagc ctggttacat taaatccttg gctttctaaa
     1321 aaatcgaggt ttttttctac tgagtcatgt tccacggcag aacttattat atgacctttc
     1381 ttcttataac aaaaaccctg aagggcaagg ttattggatt cagtggcacc agaagtaaaa
     1441 taaatttctt ctggcctagc accaataagt cttgcaatct gagtccgaac attttcaagc
     1501 ctttttcgct gctccaaacc cagtttatgg gaggaagaag gattatatcc acttaagctg
     1561 gatagttcat aaaggcttct atttagcttt tctgatgggg gacttgttgc agcatgatca
     1621 aaatacattt ttcctccttt tttactctat catgttataa tagcggggta gaaaagtaaa
     1681 ggaggggcaa tgagcaaaat cttaattatt tttaccggtg gtacaataag tatgagcatt
     1741 gataaaaatc tcggtgccgc tgttccaaac cttagtagtg atgatatatt gggaatgatc
     1801 aaaaacctag atgaatttag tgaaatcgaa gtcctaaact atggtgaatt tcccggtccg
     1861 caaatttctc ctgaaaaaat gatggaactc tccatgctta ttcaggaaaa tattaaaaga
     1921 gaggatattc aaggtgttat tttgacccat ggaacagaca ccctggagga aacagccttt
     1981 tttcttgacc ttaaccttaa gaccacaaaa cctgttgtaa ttgtaggggc catgcgaaat
     2041 gcttctgaac taggctatga tggtcctagt aatttagcgg cagctattgt gacggttaaa
     2101 agccctgaaa gcgctgatca aggagtcctt gttgtcttaa ataatgaggt caatgcagct
     2161 agggaggtta ctaaaaccaa tacccttagt cttgacacct ttaaatcaac tgaatttggc
     2221 cctcttggta ttgttgacaa caatgaggtg ctctttttta gaaaaagagt tcattatagt
     2281 caaattcttg tagatcattt tgaaaaagaa gtttatctac taaagagtta tgccggtatg
     2341 gataggtccc tgatagattt tcttgtgaaa aagggagcga ggggcattgt tattgaagct
     2401 atgggccggg gcaatctccc acccttggtg gttaaagcaa ttaaagatgc cataaaacaa
     2461 gacgttatcg tagtcattgt ttcccgctgc ccctcaggaa gagtccttgg tacctatggc
     2521 tatgaaggtg ggggaaaatt tattaaagat ctgggagcct tctttggcac aaatcttaat
     2581 ggccaaaaaa ccagaattaa acttatgctt gcccttgcct atacacagga atatagccaa
     2641 ctaaaaaaga ttatggaaag tgatagttat atatgtatct cctagaagag catatcgatc
     2701 taaatatgga tcttccccag ggttttgttt tcgatattga aactactgga ctaagtccaa
     2761 gctttaatcg agttgttatg attacctatg cctatcaaga caagaaacaa tgggtattaa
     2821 gacaagaaat ggccgaagag gaccaggatg agagcctctt gcttctaaac ttttgccagt
     2881 taagcaagaa ctttctctac gctgttcact acaatgggca aagttttgat ctacccttta
     2941 ttaataagcg attggaatat ctagcccttg ctcaaccctt tgataaaaga aggggctttg
     3001 acttatatcg ttatttgagg aaaaatgatc ataagggaag tttaaaactc ttcgatcaag
     3061 aaaaaaaagc gggttttttt agaaaagata ccctaacagg aagagaatgg gtccatcttt
     3121 ttaatcagtt taaaaaagat aaagaaaaag ctcaaagaga ggatctactt cttcacaata
     3181 aggaggatgt tttaggcact cttgctattt tgaaaaaaga aaaagccttc caagaccagc
     3241 ttcaagcccg tctttataag agtgtcctca ttttagatgc ctatccagga aaagacgaaa
     3301 tacgacttct tctttatgat ggagacgtct ccacccttga cattcctgct ctttcctatg
     3361 ataatctact ttttagcata gactattgca atgattggtc tgcacaagaa aaaagaaagg
     3421 ccctactggc atatgatgaa aaatgttttt ttgaaaacat aaagagggaa cttcagcgaa
     3481 taaagagaaa ttgtcccaaa taaaagagtg ccgcgaatcc ccttacccaa aagattcccg
     3541 gcaccccaca aatataggag ttagtttatt taatgtttct tt
//
LOCUS       DASRNA010000064         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_389691,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000064 DASRNA010000000
VERSION     DASRNA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389691"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2003
                     /locus_tag="VFD08_01845"
     CDS             <1..2003
                     /locus_tag="VFD08_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZK09790.1"
                     /translation="IILSLLGALAQFFVPINYNLADYIPDNAPSTQAMHLMEEEFDQP
                     IPNLRVYIPDLGLTQALECKEKLSEIVHVRQVLWLDDFYDIKQPIELADKELLKAYYK
                     DRGALFMVATDLDDTTRTLTEMQELIGIDGSIEGQVVDLAKAQSSVDSEMLIILLIMV
                     PLGLIILNLATSSWIEPLLLFATIGAGVLMNMGTNIVLDSVSFITAAVSAVLQLAVSM
                     DYAVFLLHRFGSLRKEGYEMEEAMELAIVRSSRAILASSSTTILGFLALVFMQFKIGP
                     DLGLVLAKGILLSLISVIFLLPVLVLYFHKAIEKTAHRSFLPSFAIAGRFVKRIRYLV
                     LILTLLAPLFFIAQQNNDFIYGTAEYPAGSREQRDQEIISDIFGDEVSLAIMVPRGEL
                     GKEKVFEEELAQIPGVLGIMSYTRNVGSLMPPEMINEDLVGALLSDNYSRINLTLKGP
                     KEGDGPFGIVEDVRRVTSKFYADQAHVLGESVINYDMKTTVEKDNVIVNGLAILSVGL
                     VLMISFKSLMIPLLLLLTIETSIWMNLSVPYFTGTKLTYIGYLVISTLQLGATVDYGI
                     LFTQHYLDNRVFRNKKDSVYHSFMETIGGLLPPALILISAGFILYYVSSLAVVSELGL
                     VLGRGALTSFLMVIFYLPGLLNMFDPLVEKLTYKAKFYKEKE"
     gene            2114..>3056
                     /locus_tag="VFD08_01850"
     CDS             2114..>3056
                     /locus_tag="VFD08_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09791.1"
                     /translation="MKNKYIKRVLSLFLALLILSPLSLVSYGLGKAEKLEKEEIIYAK
                     LDFNGENDLLIAVSGFPEGFKGKDYGDFSEVANLSTPEELVYKDGSINIDAKENFYYQ
                     GKLKTKDLPWHISMKWTLDGLPIAEEDLLGKSGHLKLVYNIRPNPACCSKYFDYYIVQ
                     SGFNFNVDQVRNLEAPDGTQATAGSVKMVNFMSFPGETSTYVLEADVDNYEPGMVQIG
                     ALPLNLALDLSEISEYTGDLKTLELAIAQLNGGTWEFLDGLYQIKEGATAYKNAGHEL
                     LSGLDQLTGGLKGLTDGSSQISEGLGDYSEGVAEFEEG"
BASE COUNT          845 a          605 c          709 g          897 t
ORIGIN      
        1 ttattatact gagtctatta ggtgcactgg ctcagttttt tgtacccatt aattacaatt
       61 tggcggatta tattcccgat aatgccccat ccacccaggc catgcattta atggaagaag
      121 agtttgacca gcccattcca aaccttcggg tttacatacc agatttaggc ctgacccagg
      181 ctctagagtg caaagaaaaa ctaagtgaga ttgtccatgt aaggcaggtt ctttggctgg
      241 atgattttta cgatataaag cagcccattg aactagcaga caaagagctc ctaaaggcct
      301 attataagga cagaggtgct ctatttatgg tggcaacaga tcttgatgat accaccagga
      361 ccctgactga gatgcaggag ctaattggga tcgatggttc cattgaaggc caggtagtag
      421 acctagccaa ggcccagtcc tcagtggata gtgagatgct cattatcctt cttattatgg
      481 ttcccttggg tttaattatc ctaaacctag caaccagtag ctggattgag cccctgcttc
      541 tttttgcaac cattggcgct ggagttctta tgaatatggg aacaaatatt gttcttgatt
      601 cggtgtcctt tattaccgct gctgtttcag cggtgcttca gctggctgtt agtatggact
      661 atgccgtttt tcttttgcac cgatttggca gcctgcgcaa ggagggctat gagatggaag
      721 aggccatgga actggccatt gtaagatctt ccagggccat cctggcctct tcttccacta
      781 caattttggg ctttttggcc ctggttttta tgcagtttaa gattggccca gacctagggc
      841 ttgtcctggc caagggaatc ctccttagtc ttattagtgt tatctttctc ttgcctgttt
      901 tggttcttta ttttcacaag gccattgaga aaacagccca ccgctccttt cttccttcct
      961 ttgctatagc ggggcgcttt gtaaagcgga ttcgctacct ggttcttatt ctcactcttc
     1021 ttgccccgct cttttttatc gcccagcaga ataatgattt tatttatggc accgccgagt
     1081 atccagcagg ctcaagggag caaagagatc aggaaattat aagtgacatc tttggcgatg
     1141 aagtttcctt ggccattatg gttcccagag gagagctagg aaaagaaaag gtttttgaag
     1201 aagaactggc tcagattcct ggtgtacttg gtattatgag ttatacaaga aatgtgggca
     1261 gtttaatgcc cccagaaatg attaatgaag atctggtggg ggccctgcta agtgataatt
     1321 atagccggat aaatcttacc ctgaagggcc caaaagaagg agatggaccc tttgggattg
     1381 tagaagatgt aagaagagta acaagcaagt tttatgctga ccaggcccat gttctagggg
     1441 agtctgttat caactatgat atgaaaacca cagtagaaaa ggacaatgtt attgtcaatg
     1501 gcctggccat tttatcggtg gggttagttc ttatgatcag ctttaagagc cttatgatcc
     1561 ccctactttt acttttaacc atagaaacaa gtatttggat gaatctttct gtgccctatt
     1621 ttacgggaac aaaactaaca tatattgggt atttagtcat tagtaccctg cagctggggg
     1681 caacggtgga ttatgggatt ctttttaccc agcactatct tgataatagg gtctttcgaa
     1741 ataaaaaaga ttcggtctac cactccttta tggagaccat tggggggcta ctacccccgg
     1801 ccctgatctt gatttctgcc ggttttatcc tctattatgt atccagtctg gctgtagtaa
     1861 gtgaactggg cctggtcctt ggtaggggcg ccctgaccag ttttttaatg gttatcttct
     1921 atctgccagg gctactaaat atgtttgatc ccctagtgga aaaattaacc tataaagcaa
     1981 agttttacaa agaaaaagaa taaaaggtat ttatttacta ggaccttttt gggtaggata
     2041 agcaaattgc agggtataaa gaagaagagc gcctgctcta gttagattta ataagaaaaa
     2101 ttcaggagga cctatgaaaa ataaatatat aaagagagtc ttatctcttt ttctagccct
     2161 gcttatacta agtcccctta gccttgttag ttatgggctt ggtaaggcag aaaaactaga
     2221 aaaagaagag attatctatg caaaactaga ttttaatgga gaaaacgacc ttcttattgc
     2281 tgttagtggt tttcccgaag gctttaaagg aaaagactat ggcgacttta gtgaggtcgc
     2341 caacttaagt acgccagaag aactggttta caaagatggt agcattaata tagatgccaa
     2401 ggaaaacttc tattatcaag gaaaactaaa aacaaaagac cttccttggc atatttccat
     2461 gaagtggacc ctagatgggt tgcccattgc agaagaagat cttttaggaa aaagcggcca
     2521 tctaaagctg gtctataata taagacccaa tcccgcttgt tgttctaagt attttgacta
     2581 ttatattgtc cagtcgggtt ttaactttaa tgtggaccag gtaagaaacc tagaagcccc
     2641 tgatgggacc caggcaactg caggctcggt taagatggtt aattttatga gttttccagg
     2701 agaaacaagc acctatgttt tagaggctga tgtggacaac tatgaaccgg gcatggtgca
     2761 aattggggcc ctgcccctaa accttgccct agatctatca gaaatctccg agtatacggg
     2821 ggaccttaaa acccttgaac tggctattgc ccagttaaat ggggggacct gggaatttct
     2881 tgatggcctt tatcagataa aagaaggagc cacggcctat aaaaatgccg ggcatgaact
     2941 tctttctggt ctagaccagc taacgggggg cctaaaaggc cttacggatg gttcttccca
     3001 aataagtgaa ggcctaggag actatagtga gggagtggca gaatttgaag agggca
//
LOCUS       DASRNA010000065         6478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_836167,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000065 DASRNA010000000
VERSION     DASRNA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836167"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..342
                     /locus_tag="VFD08_01855"
     CDS             76..342
                     /locus_tag="VFD08_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZK09792.1"
                     /translation="MAAIRLIKWGNSQGIRIPKNVLKKLNLNTDNLEDQKIKFHLEVK
                     DGQLLLSPMEEKSKLEELFLHFDGNPEDYKTSISWGEPVGKEVW"
     gene            complement(420..791)
                     /locus_tag="VFD08_01860"
     CDS             complement(420..791)
                     /locus_tag="VFD08_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZK09793.1"
                     /translation="MMQKNKRVSELDFGDIIKLDFSPTEGHEQHGYRPAVVLTDPKSQ
                     NSMLNGMTSVAPITKIKKGFPTHVLLDSQTEIRGSILVEHHRMLDLRSRNFVYVEKLP
                     LNKLQEVKRVFEALYQDLLSL"
     gene            complement(983..1360)
                     /locus_tag="VFD08_01865"
     CDS             complement(983..1360)
                     /locus_tag="VFD08_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004438469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK09794.1"
                     /translation="MLTKFGLLLRHLRLSKNQVLKDMADKIGVSSSYLSSVENGKRKV
                     PRDWADKIKSQYDLDKTQYLELLNAVDLTNKEIIISLDCKSQEQKEVILSFARKVDDM
                     DENFIKAFKELLKKQKGKTNNIR"
     gene            1724..2506
                     /locus_tag="VFD08_01870"
     CDS             1724..2506
                     /locus_tag="VFD08_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK09795.1"
                     /translation="MFEYCSLVSGSSGNCHFIKTSSVKILIDVGLSARRVTKGLEQIG
                     ESIEEIDAIFLSHVHGDHTRGLNVLFKRHNFDLYAHESVIEAMELGPSSRIYPLDGRA
                     DLKDLKIETFKLPHDAPITYGFRLSYDKRVLSIATDLGYAPEEVVQMLLPSDFLIIEA
                     NHDEDLVRVGPYPYSLKRRILGEEGHLSNEDAGRLISRIYKEEERLRFVMLAHLSEKN
                     NYPELARMSVEKILKKEGIKVGEDLLLDVAHRDHCTAVYRIL"
     gene            2513..3217
                     /locus_tag="VFD08_01875"
     CDS             2513..3217
                     /locus_tag="VFD08_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK09796.1"
                     /translation="MKKRFYGWVVVLAGFLLMATGWGIVYNTSSLFIVPISEELGLLR
                     SQITFTMTLRSLVQMMVALFAGRIYRRFSLLKSMKFMSLVLVISYILFGFATNIWQIY
                     LLTIIGSLANSFIGIIPISMLIGNWFQEKRGFALGLAFMGTGIGGMLYNSLTGRWILN
                     YGWQRSYQLQALVMFMIIVPTVFFLVKEKPQDMGLEAYGRKGPQEPSRGISLEEAKGL
                     PYFWIIALVSCVANWP"
     gene            3214..3732
                     /locus_tag="VFD08_01880"
     CDS             3214..3732
                     /locus_tag="VFD08_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK09797.1"
                     /translation="MITLAPHLVDIGYGFAFSANILALTMASLAVGKLVLGELLDRLG
                     LKGAISISIMGSVFAYIGLFNAQHKLLLILVILGTGIGCAYGTMVDPYLTRSIFGEKD
                     YSSIYGILAAAASLGGMLSPIVSGLSYDINGNYQNLLFQMGLLALALLLAAQWIFSHK
                     NMQAVKEKWDLD"
     gene            4074..5483
                     /locus_tag="VFD08_01885"
     CDS             4074..5483
                     /locus_tag="VFD08_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HZK09798.1"
                     /translation="MTKKKKREFPHTYVIIFAIIILAGVMTYVLPAGEYDRVEITMPD
                     GSSRTVVDSTTFTHVEPSPVSPWEMMQAVPKGLIEASTIVFFIFVVGGAFGIINSTGA
                     ILDGIGNLALRFGGKEKLMIPVIMTIFSLGGATFGMSEETIVFVPIGIALARSLGYDA
                     MVGTAMVFSGAAAGFVGGWMNPFTVGVAQGIAELPTFSAIGFRLVLWVTILASAIFMV
                     MRYAAKVKEDPEKSYCRELELSESQDSAALKEVNVLSGKQKLVLVGFVIGMGILIYGV
                     LVHGWYMVEIATLFFTMGIVLGAIGGVSPNKMAVEFLEGAKGLATGALVVGFSRGILV
                     VLTQGAILDTIIHGLASVISGLPKTIAAVGMFGVQSIINFFIPSGSGQAGTTMPIMVP
                     LADVIGLTRQTAVVAFQCGDGISNSIIPTGASLLAALGVANIKYESWVRFIWPLILVQ
                     SAIAAVFMVAATLMKLGPF"
     gene            5492..>6478
                     /locus_tag="VFD08_01890"
     CDS             5492..>6478
                     /locus_tag="VFD08_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZK09799.1"
                     /translation="MQKFKERALELLPELKELNQYIYDNPELGYEEFLSSQAHIKMLE
                     KHGFEIERSPLGLETAYIARYSGDKPGIKMAFLSEYDALPGIGHGCGHNLLGTTSIGA
                     ALVLKDYVKEKGGTVVLYGTPAEETSGAKVAFADEGYFEGIDVAICPHPADAYYSSGE
                     SLALHPIAFTFKGKTAHAASEPEAGVNALDALLLTFNNINALREHILPSARIHGIVKE
                     GGLAANVVPEKSVGEFYVRTMTLDYMEELLEKVINCAKAGALATGCTMEQEQYEAAYH
                     NMVTNKVLSDHYNGLLEEMGIEVSPPRDSFGSLDMGNVSQVCPSINPYFGISD"
BASE COUNT         1755 a         1249 c         1465 g         2009 t
ORIGIN      
        1 gtatatacag ggatatacta taaatacaaa agaaagataa aaagaaaggt gaagatagat
       61 taaaaggagg ggacaatggc tgctataagg ttaattaaat ggggaaacag ccaggggata
      121 cggataccaa aaaacgtttt aaaaaaacta aatttaaata cggacaacct agaggatcaa
      181 aaaataaaat tccacctaga agttaaggat ggtcaacttc tactaagtcc tatggaagaa
      241 aaaagcaagc ttgaagagtt atttttgcat tttgatggaa atcccgaaga ttacaagaca
      301 agtatttctt ggggagagcc tgtgggtaaa gaagtttggt gattagcttt taaataaagc
      361 cgctgtttat aaaggtaaac agcggctttg aaattactag aatgatttgg cattaactac
      421 tataggctta gtaaatcttg atataaggcc tcaaaaactc ttttcacttc ttgaagttta
      481 tttaagggaa gtttctcaac ataaacaaag tttctgctac gaagatcaag cattctgtga
      541 tgttccacta aaatagatcc ccgaatttca gtctgtgaat ccagtaaaac gtgggttggg
      601 aacccttttt ttatttttgt tataggagca acagaggtca tgccatttaa catgctgttt
      661 tgacttttgg gatctgttaa aaccactgct ggtctatagc cgtgctgctc gtggccttct
      721 gtaggggaaa agtctaattt aataatatca ccaaaatcca actcgcttac ccttttattt
      781 ttttgcatca tctaggcttg tcctttctaa aaaaccttta ctctcatatt ttgcagtatt
      841 gttttgcgtg ctagccttgc ttaccagctt gtctagaaat aagtaggggc tccttgatag
      901 ttatggtctt attgctacta ttatttttgc taaagctatt tggaaaaact gacttgagcg
      961 actcttccta ttttccccta atctacctta tattatttgt cttgcccttt tgttttttaa
     1021 gaagttcctt aaaggctttg ataaagtttt cgtccatgtc atcaactttt ctggcaaaag
     1081 ataatatgac ttctttttgt tcttgggact tacaatctaa agatatgata atttctttgt
     1141 ttgtcagatc gactgcattt aggagctcta gatattgagt tttgtctaaa tcatattggc
     1201 ttttaatttt atcagcccag tctcttggga cttttctttt gccattttct accgatgata
     1261 ggtaggatga agagacacct attttatcgg ccatatcttt taacacttga tttttgctta
     1321 atcttagatg tcttagcaag agtccgaatt ttgttagcat agcagtcctc ctagtcttgc
     1381 gaatgaataa gcctttctat acaaaacagt atcatagttt cacagatttg taaaactatt
     1441 tctgaaaaag caaatatggt tttgccctag cctgtagtct atggcaagag ttgatactat
     1501 aataaaggaa gatctatatg gaatcgattt atgcaagctt atcttgcgta ggatttacag
     1561 gataagcttt cctcttaaaa ggacagctta tgacaggttt tctaaatatt atttttattg
     1621 gatttataaa gggctggtaa attagaccgt ccacttcttg ctttggacct aagctacttt
     1681 tttttgtccc tatactctgt tacaatacaa gaggagaaaa tatatgtttg aatattgttc
     1741 gcttgttagt ggcagttcag gaaactgcca ctttattaaa acttcatcag taaaaatcct
     1801 cattgatgtg ggccttagtg cccgcagggt gactaagggc ctggagcaaa tcggagaatc
     1861 catagaagag atcgatgcta tttttcttag ccacgttcat ggagaccata ccagggggct
     1921 caatgtgctt tttaagcgcc ataattttga tctttatgcc catgagagtg tgattgaggc
     1981 catggaactt ggtcctagct ctcgcattta tcctcttgat gggcgggcgg atctcaagga
     2041 tcttaagata gaaaccttta agctgcccca tgacgccccc atcacctatg gttttcgcct
     2101 aagctatgat aaaagggtcc tcagcattgc cacagacctg ggctacgccc cagaggaagt
     2161 ggtgcagatg ctcttaccca gtgatttttt aattattgag gccaaccatg atgaggacct
     2221 tgtaagggta ggcccctacc cctatagtct taaaagaaga atactaggag aagagggcca
     2281 tctatccaat gaggatgcag gcaggcttat ctctagaatt tataaggaag aagagcgcct
     2341 gcgctttgtt atgctggccc atcttagtga aaaaaacaat tacccagaac tggcccgaat
     2401 gagtgtggag aaaattttaa agaaagaggg aataaaggtg ggagaagatc tactcttaga
     2461 tgttgcccac cgagaccact gtactgcggt ctatcgaatt ctataaagta aaatgaaaaa
     2521 aagattctat ggttgggttg tggtccttgc gggctttctt ttgatggcta caggctgggg
     2581 cattgtatat aatacttcta gcttatttat tgttcctatt agtgaggagc taggacttct
     2641 gcgctctcag atcaccttta ctatgaccct aaggtcctta gtacaaatga tggttgcgct
     2701 ttttgccggt agaatttacc ggcgtttttc ccttcttaag tccatgaagt ttatgagtct
     2761 ggtcctggtt ataagctata tcttatttgg ctttgccaca aatatatggc agatctatct
     2821 tttgaccata attggctctc tggccaatag ttttatcggt attatcccca tatccatgct
     2881 aattggaaat tggttccagg aaaaaagagg ttttgccctg ggcctggcct ttatgggcac
     2941 tggtataggg ggcatgcttt ataatagcct gacaggaagg tggatcctaa attatggctg
     3001 gcaaagaagc taccagcttc aggccctggt tatgtttatg attattgtgc ctacggtatt
     3061 ttttttagtc aaggaaaaac ctcaagatat gggacttgag gcctatggaa gaaaagggcc
     3121 ccaggagccc tctaggggaa tcagcctgga agaggctaaa gggctgccat acttttggat
     3181 tattgccctt gtttcctgtg tggccaactg gccatgatta ccctggcccc tcatcttgtt
     3241 gatattggct atggttttgc tttttctgcc aatattctgg ccctaaccat ggcctcccta
     3301 gcggtaggaa aacttgtttt gggggaactt ttagatcggc tgggcctaaa aggggctatt
     3361 agtatttcta ttatgggttc tgtttttgct tatattggtc tttttaatgc ccagcataag
     3421 cttcttttga tcctagttat tctaggtact ggtattggtt gtgcctatgg gaccatggtg
     3481 gatccttatt taaccaggag catatttgga gaaaaagatt atagcagcat ctatggcatt
     3541 ttagctgcag cagccagctt aggtggtatg ctttctccta tagtaagtgg gttaagctac
     3601 gatataaatg gcaattatca aaatttactc tttcaaatgg gcctgctagc cctagccctt
     3661 cttttagcgg cccagtggat tttttcccat aaaaatatgc aggcagtaaa ggaaaaatgg
     3721 gacctggact aggggtttaa gagaatgggg tgaatttttc ctagactggc gttttttatg
     3781 atttggaaaa aaattgattt ttatgctcta aaccagcacc cagggggact ttattagcta
     3841 agttcccttt tttactgctt atatttaagg taaaaagtta tttttcccct ttgggtaaaa
     3901 aagatgctag aaaagggcaa agggagctct tttttacgag attatcgtga tttgtctggt
     3961 atttgttttg ggaaataaat aacaaaaatc tataataaga aattgtcatc tgcttgatat
     4021 tgaaagcgaa tatcatatat gttaagatta atttatcata aaaggaggtt gatatgacta
     4081 aaaagaaaaa gagggaattt ccgcatactt acgtgattat ctttgcaatt attatcttag
     4141 ccggtgttat gacctacgtt ttgcccgcag gcgagtacga tagggtagaa attactatgc
     4201 ctgatggctc aagtcgaact gttgttgatt ctactacctt tacccatgtg gaaccaagtc
     4261 cggtttctcc ttgggaaatg atgcaggcgg tacccaaagg attgatagaa gcctcgacca
     4321 ttgtgttttt tatctttgtt gttggagggg cctttgggat tattaacagc accggcgcca
     4381 ttttagatgg cattggcaac ctggcccttc gctttggagg aaaagaaaaa cttatgatcc
     4441 cagtgattat gaccattttt tccctgggag gagctacctt tggaatgagt gaagagacca
     4501 ttgtctttgt tcccattggc atcgccctgg ccagaagcct aggctatgat gcaatggtgg
     4561 gaacagccat ggtctttagt ggggccgctg ctggttttgt tggtggctgg atgaacccct
     4621 ttaccgttgg agtggcacaa ggaattgctg aactacctac cttctcagcc attggttttc
     4681 gtctggttct atgggtgact attcttgctt ctgctatttt tatggttatg cgctacgcag
     4741 caaaagtcaa agaagaccca gaaaaaagct attgtagaga acttgaatta agtgaaagcc
     4801 aagatagtgc tgcccttaaa gaagtaaatg tccttagtgg aaaacaaaaa ctagtcctag
     4861 ttggctttgt tattgggatg ggaattctaa tttatggagt tcttgttcat ggctggtata
     4921 tggtagaaat tgccacacta ttttttacca tgggtattgt cctaggagcc attgggggcg
     4981 tatctcccaa caaaatggcc gttgagtttt tagaaggagc caagggacta gcaacaggag
     5041 ccctggttgt tggtttttcc agagggattc ttgttgtact aacccaagga gccattctag
     5101 ataccattat tcacggactg gcctcggtga tttcaggcct acctaagacc attgctgcag
     5161 taggaatgtt tggggttcaa tctattatta acttctttat tccttctggt tcaggacaag
     5221 caggaaccac catgcccatt atggttcccc tggcagatgt tattggttta actcgccaga
     5281 cagccgttgt ggcctttcaa tgtggtgatg ggatctccaa tagtattatt cctaccggag
     5341 cctccttact tgctgcccta ggtgtggcta atattaagta cgagtcctgg gtccgcttta
     5401 tttggcccct gatcttagtg cagtctgcca ttgctgcagt ctttatggtt gctgccaccc
     5461 tgatgaaact tggacccttc taggaggagt gatgcaaaag tttaaagaaa gagccttaga
     5521 gctccttcct gaattaaaag aattaaatca atatatatat gataaccctg agctgggcta
     5581 tgaggagttt ctttccagcc aggcccatat caagatgctg gaaaagcatg gctttgagat
     5641 tgagcgctct cctttagggc tcgagactgc ctatattgcc cgctactctg gagataaacc
     5701 gggcataaag atggcctttt tatctgagta cgacgccctg ccaggaattg gccatggctg
     5761 tggccacaat ctactgggaa ccacatccat tggagcggcc ctggtcctaa aggactatgt
     5821 aaaagaaaaa ggcgggacag tagttcttta tggaactcct gctgaggaaa ccagcggggc
     5881 caaggtggcc tttgctgatg agggatattt tgaggggata gatgtagcca tctgtcccca
     5941 cccagccgac gcctattatt catcaggaga aagcctggcc ctacatccca ttgcctttac
     6001 ctttaagggc aagacggccc acgctgcctc agaacctgag gccggggtca atgccctgga
     6061 cgccctgcta ctgaccttta acaatatcaa tgccctaaga gaacatatct tgcccagtgc
     6121 aagaatccac ggcattgtta aagagggagg gctagcagcc aatgttgttc cagaaaaaag
     6181 tgtaggagag ttttatgtac ggaccatgac cctggactat atggaggagc tgctagaaaa
     6241 ggtgatcaac tgtgccaagg cgggagccct agccacagga tgcaccatgg agcaggagca
     6301 gtatgaggct gcctaccaca atatggtgac caataaggtt ttatccgacc actataacgg
     6361 cctacttgaa gaaatgggaa tagaggtcag tcctcctagg gatagctttg gctcccttga
     6421 tatgggcaat gtatcccagg tctgtccttc cattaatcct tattttggca tttcagac
//
LOCUS       DASRNA010000066         5505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_166943,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000066 DASRNA010000000
VERSION     DASRNA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166943"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFD08_01895"
     CDS             complement(<1..719)
                     /locus_tag="VFD08_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09800.1"
                     /translation="MLDSIKERREIGLEYVFEKLQIMTPYGEKRQAARKVYRPEEKKE
                     LEKELEAVELFTKELSLKAYPLHELQYEFSRIKEISGSIKRADRGAILDEVELFEIKT
                     FSRVVDELRQKTQKLKTPQGFELKSLDKVAKALDPENSGLLTFYIYDSYSKKLEQIRL
                     EKKSLEERIYRKAEDQRNELFLKRADLVVEEEKEERRIRSELSKIIAGHSEIFVHNMQ
                     KIGELDHWIAKAKLARSYPSC"
     gene            complement(709..1734)
                     /locus_tag="VFD08_01900"
     CDS             complement(709..1734)
                     /locus_tag="VFD08_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09801.1"
                     /translation="MLIDKIRQLNSLAIIGMDKNAGKTTVLNHLIQEAKAVGFPSTLA
                     ITSIGRDGEEKDVVLSSLKPRIYVYPGSLVATSRDLLKHCDLTKEILETTGINTPTGE
                     VILFRAKSAGFVEIAGPSSIQGAQRVKESFLKIDPNCFYLVDGALSRKSTAGHFLTDG
                     AILATGASSSSSMTKVVDRTITMVEIFKSPKLEEKYMQEVKTKLEKARLVIFGKETTL
                     LPVELAFTAAREAAEKIDKSTYLIAVKGVITDSFLNSLMIGADLRGKTLLVEDATRLL
                     ISSELWRKVKSLGIKVRVLQKIDLVAITINPFSPRGLHFDSYAFEEALSRETNLFIYD
                     VGRSYAR"
     gene            complement(1724..2983)
                     /gene="ablA"
                     /locus_tag="VFD08_01905"
     CDS             complement(1724..2983)
                     /gene="ablA"
                     /locus_tag="VFD08_01905"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HZK09802.1"
                     /translation="MNLEAEQRRAKLFPNVSDEDWKSWKWQVKNRIETIEDLKKFIPL
                     TAEEEEGVKTTLETLRMAITPYYLSLIDPEDPHDPVRKQAVPTVAEVHRSPWDLLDPL
                     SEDEDAPVAGLTHRYPDRVLFLLTDQCSMYCRHCTRRRFAGQTDSPVGKSQIDAGIEY
                     IANTPQIRDVLLSGGDALLMSDDRLEDIIKRLRAIPHVEIIRIGSRAPVVMPQRITED
                     LVNMLKKYHPIWLNTHFNHPNEITTESKEACERMANAGIPLGNQSVLLRGVNDCVHVM
                     KELVQKLVYIRVRPYYIYQCDLSFGLEHFRTSVSKGIEIIEGLRGHTSGYAVPTFVVD
                     APGGGGKIPVMPNYVISQNQNRVVLRNYEGVITTYTEPDCYTPDCSCDICTGKKEGKL
                     DGVAGLLAGERLALEPDHLARKERHVD"
     gene            complement(3001..4038)
                     /locus_tag="VFD08_01910"
     CDS             complement(3001..4038)
                     /locus_tag="VFD08_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008796589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HZK09803.1"
                     /translation="MNLACRYGTHRVIEPKGVLPQAAQKIDNNMEIFDNEILLDVTAL
                     NIDSASFTQIANACDHDVEKIKKMITDIVDDKGKMQNPVTGSGGMLIGRVKKIGPALK
                     DKVTIKEGDKIATLVSLSLTPLKIDKIKKIYPEIDRVEIEGSAILFESGIYAKLPDDM
                     SENLALAALDVAGAPAQTAKLVRPGDKVLIVGGGGKSGMMCVYEAKKRVGPTGRVVAL
                     EGNEVVKERLASLGFADEIIVANAQDPMEVLEKALAANNGQEYDVSINVVNVPNAEMS
                     CILPVRNGGTVYFFSMATSFTKAALGAEGVGKDVTMIVGNGYTKDHAEITLEELRESD
                     KLRAIFEKLYL"
     gene            complement(4040..4861)
                     /locus_tag="VFD08_01915"
     CDS             complement(4040..4861)
                     /locus_tag="VFD08_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HZK09804.1"
                     /translation="MEKLIITAAICGAEVTKEHNPAIPYTVEEIAREAKSAYDAGASI
                     IHLHVREDDGTPTQDKARFQACIEAIREVAPDVIIQPSTGGAVGMTDLERLQPTEIEG
                     TEMATLDCGTLNFGGDEIFINTENTIKNFGKIMIERGIKPEIEVFDKGMIDYAIRFEK
                     QGFIKKPMHFDFVLGIQMAATARDLAFMVDSIPAGSTWTVSGVGRHEFPMAAIGIAMG
                     GHVRVGFEDNVYLDRGVLAKSNGELVERVVRIAKELGREIASPAEARKILSLEER"
     gene            complement(4864..5244)
                     /locus_tag="VFD08_01920"
     CDS             complement(4864..5244)
                     /locus_tag="VFD08_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HZK09805.1"
                     /translation="MESTIRLRMSNADAHYGGNLVDGAKILQLFGDVATELLIRRDGD
                     EGLFVAYDMVEFTAPVYAGEFIEAKGRIVKEGSSSRKMEFEAYKVIVPRADLSDSACD
                     VLEEPELVCRAKGTCVVPKSKQRR"
     regulatory      complement(5301..5472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         1494 a         1285 c          983 g         1743 t
ORIGIN      
        1 atacaagaag gatagcttct ggcaagcttg gctttggcaa tccagtgatc aagctcacct
       61 atcttctgca tattatgaac aaatatttca ctatggccag ctataatctt agaaagttcg
      121 ctgcggatgc ggcgttcttc tttttcttct tccactacaa gatcagctct ttttaaaaag
      181 agttcatttc tttgatcctc ggctttacga taaattcttt cttctaggga ctttttttcc
      241 aggcgaatct gctcaagctt tttgctatag ctatcataaa tataaaaagt taaaagtccg
      301 ctattttctg gatcaagagc ctttgctacc ttatcaaggc tttttaattc aaagccctga
      361 ggggttttta gcttctgcgt tttttgccta agttcatcga ccactcgaga aaaagtcttt
      421 atctcaaaaa gttccacctc atctaatatg gctccccgat cagctctttt aatagagcct
      481 gaaatttctt tgatccttga aaactcatac tggagttcat gaaggggata ggccttaagg
      541 gagagttcct tagtaaagag ttctactgcc tctaactctt tttcaagctc ttttttttct
      601 tctgggcgat agacttttct tgctgcctga cgtttttccc catagggcgt cataatctga
      661 agtttttcaa atacatactc cagaccaatc tctcttcttt ctttaatact atctagcata
      721 agacctccct acatcataga taaaaagatt tgtctcacgg ctaagggcct cttcaaaggc
      781 atagctatca aagtgaaggc cacgagggga aaagggatta atggtaatgg ccacaagatc
      841 aattttctga agaaccctga ccttgattcc taaactcttt acctttctcc atagttcact
      901 actaataagt agcctggtgg catcttccac cagcaaggtt tttcctctaa gatcagctcc
      961 aatcatcaga gaattcaaaa aggagtctgt aatcacaccc ttaactgcaa ttagataagt
     1021 gcttttgtca atcttctccg ctgcctctct agcagcagta aaagcaagct ctacaggaag
     1081 aagggtggtt tcttttccaa aaattaccag ccttgctttc tctagcttgg tttttacctc
     1141 ttgcatatac ttttcttcca acttgggact tttaaaaatt tctaccatgg tgatcgtcct
     1201 atctaccacc ttagtcatag aagaagagga actagccccg gtggctaaaa tcgctccatc
     1261 ggttagaaag tgacctgctg tactctttct tgaaagagca ccatctacca aataaaaaca
     1321 attgggatca atttttaaaa aggactcctt taccctctgg gctccttgaa tacttgaggg
     1381 ccctgcaatt tcaacaaagc cagcactttt agctcgaaaa agaatcactt cccctgttgg
     1441 ggtgttgatg ccggtagttt ctaaaatttc tttggtaaga tcgcagtgtt ttaaaagatc
     1501 cctgcttgtt gctaccaggc ttcctggata aacatagatt cttggcttaa ggctggagag
     1561 gacgacatcc ttttcttccc catctctgcc aatactagta atagccaagg tggagggaaa
     1621 acccaccgcc ttggcttctt gtattaaatg attaagaacc gttgtctttc ccgcgttttt
     1681 atccataccg ataatggcaa gagagttaag ctgtcttatc ttatcaatca acatgtcttt
     1741 cttttcgtgc tagatgatct ggctctaggg ccaatctttc ccctgcaaga agaccagcaa
     1801 caccgtctaa tttgccttct tttttacctg tacagatatc gcaactacaa tcaggagtat
     1861 agcagtctgg ctcggtatag gtagtgataa cgccttcata gtttcttaaa acaaccctat
     1921 tttgattttg gctaataaca tagttgggca taacaggtat cttaccacca ccgcctggag
     1981 catctaccac aaaggttggc actgcatatc cagaggtatg gccccttagc ccttcaataa
     2041 tctcgatgcc cttagataca gaagttctaa agtgctcaag accaaagctt aagtcgcact
     2101 ggtaaatata ataggggcgg actcttatat aaactagttt ttgtactagt tctttcatca
     2161 catgaacaca gtcattgact ccgcgaagta gaacagactg gtttcctagg ggaattcctg
     2221 cattagccat acgctcgcaa gcttccttag attctgttgt tatttcattg ggatggttaa
     2281 agtgagtatt taaccaaatg ggatgatatt ttttcagcat atttactaaa tcttctgtaa
     2341 tacgctgggg cataactact ggcgcacggc tgccaatgcg gattatttcc acatggggta
     2401 tggcgcgcag tctttttata atatcttcca aacgatcatc actcattagc agtgcatccc
     2461 caccagaaag aaggacatca cgaatttgcg gggtatttgc aatatattcg ataccagcat
     2521 caatctggct ctttcctaca ggagaatctg tttgtccggc aaatcttcta cgtgtacagt
     2581 gacggcaata catgctgcac tggtctgtta gaagaaatag tacacgatct ggatatctgt
     2641 gagttagacc agctacagga gcatcttcat cttcacttag ggggtctaag aggtcccaag
     2701 gactacgatg tacttcagca actgttggaa cagcttgctt gcgcacagga tcatgaggat
     2761 cttctgggtc aattaatgat agatagtaag gagttatcgc catgcgaagg gtttcaaggg
     2821 ttgtcttaac tccttcttct tcctcggctg ttaaggggat aaacttcttc aaatcttcga
     2881 tggtctcaat acggtttttt acttgccatt tccagctttt ccagtcctca tcagatacat
     2941 tgggaaagag ctttgctctt ctttgttcag cttctaaatt catatttctc tcctttttcc
     3001 ttatagatag agtttctcga agattgcacg aagtttgtct gactctcgaa gttcttccaa
     3061 agtgatttct gcatgatcct tggtataccc atttcctaca atcatggtta catcttttcc
     3121 taccccttca gctccaaggg ctgcttttgt aaagcttgtc gccatactaa agaagtatac
     3181 cgttccccca ttgcgcacag gaaggatgca actcatttca gcattgggaa catttactac
     3241 attgattgaa acatcgtatt cctggccatt attagcagcg agtgcttttt caagaacctc
     3301 cataggatct tgtgcattgg ctacaataat ctcatcggca aagcctaggc tggcaagacg
     3361 ctcttttact acttcattgc cctcaagggc aaccactcta cctgtggggc caacgcgttt
     3421 tttcgcttca tagacgcaca tcataccgct ttttcccccg ccaccaacaa taagaacctt
     3481 gtcgccaggg cgaacaagct ttgctgtttg tgccggtgca ccagccacat caagggcagc
     3541 tagggcaagg ttttctgaca tatcatcggg aagtttagca taaataccag attcaaaaag
     3601 aatggctgaa ccttctattt caacccgatc aatctcagga tagatttttt taattttatc
     3661 tatttttaga ggtgtaaggg aaagactaac caaggtagca attttgtcgc cttctttaat
     3721 agtgacctta tccttaaggg caggaccaat ttttttcact ctacctatta gcatgccacc
     3781 actacctgta acagggtttt gcatctttcc tttatcatca acaatatctg ttatcatttt
     3841 tttgattttt tctacatcgt gatcacatgc attggcaatt tgcgtaaagc ttgccgagtc
     3901 aatatttaga gcagtgacat caagaagaat ttcattgtca aaaatctcca tattgttgtc
     3961 aattttctgc gcagcttgag gaagaactcc cttaggttca attactcgat gtgtcccata
     4021 cctacaggcc aagttcatat tatctttcct ccaaacttag aatttttcta gcttcagcag
     4081 ggctagctat ttctctgcct aattccttag caattctcac aacacgctct acaagctcac
     4141 cattgctttt agcaagaaca ccacggtcaa gataaacatt gtcttcaaat ccaactctta
     4201 catgcccccc catggcaatg cctatagcag ccataggaaa ctcatgacga ccaacaccag
     4261 aaacagtcca ggtgcttccc gcaggaatac tgtctaccat aaaggctaag tctcttgctg
     4321 tagcagccat ttggatacca agcacaaaat caaagtgcat aggtttttta ataaaacctt
     4381 gtttttcaaa gcgtatggca taatcaatca tacccttatc aaaaacttca atttcaggct
     4441 taatgccccg ttcaatcata atttttccaa agttttttat cgtattttct gtatttataa
     4501 agatttcatc gccaccaaag ttaagggttc cacaatcaag agtagccatt tcggttcctt
     4561 ctatttctgt gggttggagt ctttctagat ctgtcatacc aaccgcaccg cctgttgaag
     4621 gctgaataat cacatcagga gcaacctcgc gtatggcttc aatacaggcc tggaagcgag
     4681 ccttatcttg agtaggagtt ccatcgtctt cacgaacatg aaggtggata atactagctc
     4741 ctgcatcata ggcactcttt gcttctctag cgatttcttc tactgtgtaa ggaattgcag
     4801 ggttatgttc ttttgttact tcggcaccgc aaatagcagc ggtaataatt aatttttcca
     4861 ttcttacctc cgttgtttgc tcttaggaac aacacaggtt cccttagcac gacatacaag
     4921 ttcaggttct tctagaacat cacaggcaga atcacttaag tctgctcttg gaacaatcac
     4981 cttataggct tcaaactcca tttttctgct gctagaacct tctttaacaa ttcgtccctt
     5041 tgcttcaatg aattctcctg cataaacagg tgctgtaaat tctaccatgt cgtaggctac
     5101 aaatagtccc tcatcaccat cacgtcgaat cagtaattct gttgccacat ctccaaaaag
     5161 ttgcaatatt tttgctccat caactaagtt accgccatag tgggcatcag cattgctcat
     5221 ccttaatctg attgtagatt ccatttcttc ctccttaaat taggcttaaa aaaagcccta
     5281 catgttttat ttttcaacaa aacataaata gcgctccatg atagtcatga cagtaaaaag
     5341 gcattaaccc tttcaccaag aaaaaaaccg gacaaaacat ttttctttcg gcaattaacc
     5401 cttcgtaacc taccacgact gtccaatcgt attgggaagc tactaccctt taaatgcacc
     5461 tctatttact tcttcttgat tagttttatt atactataga taagg
//
LOCUS       DASRNA010000067         6307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_686544,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000067 DASRNA010000000
VERSION     DASRNA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686544"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1936
                     /gene="pknB"
                     /locus_tag="VFD08_01925"
     CDS             38..1936
                     /gene="pknB"
                     /locus_tag="VFD08_01925"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZK09806.1"
                     /translation="MIGNILKGRYEIIEELGIGGMGIVYLAYCSYLKRRVAIKVLLQD
                     NQGKEETLLQEAKAAARLSHGNIVQIYDIFEEGNKTYIVMEYVRGKSLRQLISNQEGT
                     PFTEQRALQLGMKLASALHQAHLNGVIHRDIKPDNILIDQDGEPKITDFGIARVSNDT
                     TIVHTDEILGSLRYSSPEQLRGNIIDERADIFSLGIVLFEMLTGIMPFPDDSPVTAAF
                     KKLKQRIPKVSTINPEVSLQTEAIVSKATSLDPKERFRSMSEFYYALSAALDTPKTYS
                     KNTPLTPRKKKKPSRVVGETNKKDPQPSKNSGVFMVLIGLLLALVTVVLIAVPLLGGE
                     SLEKEVEVPSFENILIKDAQAILDKNKLIGIVNEAVFDSSPKGTILSQDPEWGSMVEI
                     GSTIYFKVSKGQEIVLVPSIIGLNVEEARDLLYESRLELGEIKGEFDNTIEKGIIMDQ
                     EIDPDTEVAPLTVINLTISDGPEEINSIVPQMIGFQLSIAVERAQNSDIKVLVQETHN
                     EAPVGEVIAQSLKEGTEVKKDSSVTITVSKGPMQEVEEEPKQIDYAFIVDPTTFSAGI
                     DGAAIEEYYFRAVLTIDGKDSELFNEFHEVSEGIVSSVYPAPQGASYALYVDALGQTY
                     QVGAGVIQ"
     gene            1933..2778
                     /gene="rsgA"
                     /locus_tag="VFD08_01930"
     CDS             1933..2778
                     /gene="rsgA"
                     /locus_tag="VFD08_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZK09807.1"
                     /translation="MTGRIFKAIAGDYWVETEKGSIVCRPRGVFRHEGIKPAVGDMVE
                     IKDQTITKVLARKNLLLRPHVANVDKALLVFSLSEPKPDLLTLDTLIVNVLYEGIEPV
                     LVFNKSDLVQSEDIKKMAGLYSSFSKYFVSMYNKDSLEDFYQELSSGVYVLAGPSGAG
                     KSSMINALSGKDLFVVGELSAKIKRGKHTTRHNELIYLEKGIFLADTPGFQNLTLTSM
                     DVTELPEYYPEFQKVNSCRYDNCLHDKEPACGVKKAVLEKVIVESRYKNYLLLLDELR
                     SRKDY"
     gene            2778..3416
                     /gene="rpe"
                     /locus_tag="VFD08_01935"
     CDS             2778..3416
                     /gene="rpe"
                     /locus_tag="VFD08_01935"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZK09808.1"
                     /translation="MIIAPSILSADFSRLGEEILALEKAGADWIHFDVMDGQFVPNIS
                     FGSKILEDIKALTNLPFDVHLMVKEPYNYLEDFVKKGADSITFHIEAVSDPYRYIKKC
                     RELGIKVGLSISPDTAVAEINPFLDKIDLVLVMSVYPGFGGQKFISSSLEKIQELEKL
                     RKTKGYSFLIEVDGGVGPSNAKEISSAGADILVAGTSILASKDYKISIEQLR"
     gene            3413..3991
                     /locus_tag="VFD08_01940"
     CDS             3413..3991
                     /locus_tag="VFD08_01940"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HZK09809.1"
                     /translation="MTVIIGSAPLEEALLLSYLKEASRIIAVDGGLNHLESVGQKADI
                     VLGDFDSYQGELPHGAKVFSKEKNFTDMQAALALIKEEETVILGATGGRLDHFLSVLT
                     LMRGRENIQLIDKQNRIFYRKGSFNLKKEEGYFSLFPLQDTLITIEGAKYNLDKAHVS
                     SDDSLLLSNEWLGDVTIKTDKGWVLVVLSLDK"
     gene            complement(4014..4208)
                     /gene="rpmB"
                     /locus_tag="VFD08_01945"
     CDS             complement(4014..4208)
                     /gene="rpmB"
                     /locus_tag="VFD08_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZK09810.1"
                     /translation="MARKCFVCGKSNISGHKVSHALNHTKRVWKPNLRRVRILDDNNT
                     VRREMVCSRCLRSNKVRRAV"
     gene            4343..4699
                     /locus_tag="VFD08_01950"
     CDS             4343..4699
                     /locus_tag="VFD08_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZK09811.1"
                     /translation="MKQKQITKYGSIKIDKKVIEDLARKAASECYGVLAIVDVGSMDS
                     VIQKLKKETISGGVRASEGEEGLILDVYVSLQYGVRTAAVAENIMETVKYQIESTLEL
                     PVEEVNVFIQSINVGE"
     gene            4699..>6307
                     /locus_tag="VFD08_01955"
     CDS             4699..>6307
                     /locus_tag="VFD08_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZK09812.1"
                     /translation="MEIMQLDDKTLRSMLRAGYYKLNADKEMVNSLNVFPVPDGDTGT
                     NMSLTLKTAIEMVDKEDSKQTGEILRSFSRGSLMGARGNSGVILSQIFGGLARGTENT
                     KTLGVKDFIKALDESVEVAYKAVMKPVEGTILTVIRQSTQWIKEVEGVELLDFETFFA
                     LLLEKAQLSLKGTPDLLPILKQSGVVDAGGQGLCSIFEGFLASIRGEEVLFAEENDAI
                     EHTAYHPEATDIHFAYCTEFILRAREKSNEKLKEKILEMGDSVVYVQDEDIVKIHLHT
                     NNPGVALETALEYGSLLNIKIDNMKIQHETIMGIDHKEEENIIIEEEIKNVAVIAVAA
                     GKGFKDIFTDLGVSAVIEGGQTMNPSTEDIMAEVDKIKANHILILPNNSNIILAAQQA
                     QNMSQRSIHVVETKTIPQGIAAMMGFDPFGDIEENIESMEDFFDVSTIQITYSVRNTK
                     IQGFVIKEKDILCIKDGEIVAVEKTPEKALRKVLELYSSTYDLITIYGGEESSSGRTK
                     KIIDEIQKKNEEIEIELYQGDQPVYYYI"
BASE COUNT         2077 a         1045 c         1357 g         1828 t
ORIGIN      
        1 gagataatat tgctgttgtg gtagtaaagg cataattatg attggaaata tattaaaagg
       61 acgttatgaa attatagaag agctaggtat aggcggaatg ggtattgttt accttgctta
      121 ttgtagttac ttaaaaagac gagtagctat aaaggtgctt cttcaagata accagggcaa
      181 ggaagaaact cttctacaag aggcaaaagc agcagcccgc ttatcccatg gaaatattgt
      241 acaaatttat gatatttttg aagaaggcaa taagacctat attgttatgg aatatgtgcg
      301 aggaaaaagt cttcgccaat taattagcaa tcaggaaggg acccctttta cagaacaaag
      361 ggcgcttcaa ttagggatga agcttgcttc tgccctccat caagctcatt taaatggtgt
      421 tattcatcgg gatataaagc ccgataatat actgattgat caagatggag aacctaaaat
      481 cacagacttt ggcattgcca gagtttctaa tgatacaacc attgtccaca cagatgaaat
      541 tctgggctca ctacgctact cctcgcccga gcaactaaga ggaaatatta ttgatgagag
      601 agcagatatc ttttccctag gcattgtgct ttttgaaatg cttacaggaa ttatgccctt
      661 tcccgatgat tcaccagtta cggcggcctt taaaaaatta aaacaaagga ttcctaaggt
      721 ttctacgata aatccagaag taagcttaca aacagaagcc attgtttcta aagctacatc
      781 cctagacccc aaagaacgct tcaggagtat gagtgagttt tattatgctc tttctgccgc
      841 actggataca cctaaaacat attctaaaaa cacccctcta actcctagga agaagaaaaa
      901 acctagtagg gttgtagggg aaacaaataa aaaagatccc cagccttcta aaaactcagg
      961 agtctttatg gttctaatag ggctactttt agcccttgtt acggtggttc ttattgctgt
     1021 accccttctg ggaggggaaa gtttagaaaa agaagttgag gttcctagct ttgagaatat
     1081 tttaataaaa gatgcccaag ctattttaga caaaaacaaa ctaattggta tagtcaatga
     1141 ggcagttttt gattcaagcc ctaagggtac aattcttagt caagaccccg agtggggaag
     1201 tatggtagaa attggatcta cgatttactt taaggtaagt aagggccagg aaattgtgct
     1261 tgttccttca atcattggtc taaatgtaga agaagctaga gatcttcttt atgaaagccg
     1321 tctagaattg ggcgagataa aaggggaatt tgataatacc attgaaaaag ggattattat
     1381 ggaccaagag attgaccctg atacagaagt ggccccctta acagtgatta acctcactat
     1441 tagtgatggg ccagaagaga taaattctat cgtgcctcaa atgataggtt ttcagttatc
     1501 tattgcggta gaaagagctc aaaactctga tataaaagtc cttgtgcaag aaacccataa
     1561 tgaagctcct gttggtgagg tgattgctca atcccttaaa gaggggacag aagtaaaaaa
     1621 agatagtagc gttaccatta ctgtttcaaa aggacccatg caagaggtag aagaagagcc
     1681 caagcaaata gattacgcct tcattgtgga tccgaccaca ttttcagcag gaattgatgg
     1741 tgctgctatt gaagaatatt attttagagc agtactcact atagatggaa aagacagcga
     1801 gctctttaac gaatttcatg aggtttcaga gggtattgta agtagtgtat accctgcgcc
     1861 tcaaggggcc tcttatgccc tatatgttga tgccctgggc cagacctatc aagttggtgc
     1921 tggtgtgatt caatgaccgg gcgaattttc aaagccattg caggagatta ctgggtagag
     1981 acagaaaaag gatctatcgt atgtcgtccc agaggtgttt ttagacatga aggcataaag
     2041 cctgccgttg gtgatatggt tgaaattaaa gaccaaacca taacaaaagt tttagcaaga
     2101 aagaacctac tacttcgtcc ccatgtagcc aatgttgaca aggcccttct tgtatttagt
     2161 cttagtgagc ccaaacctga tttgctaacc ttagacacct tgattgtcaa tgttctgtat
     2221 gaaggcattg aacctgtctt agtctttaac aaatcagatt tagtccaatc tgaagatata
     2281 aagaagatgg caggtttgta ctcttctttt tctaagtatt ttgtgtccat gtataataaa
     2341 gattcattag aagactttta ccaagagctt tcttctggag tttatgtatt agctggtcct
     2401 tctggagcag gaaaaagctc aatgattaat gccttgagtg gtaaagatct ttttgtcgtt
     2461 ggtgaactaa gtgccaaaat aaaaagagga aagcatacta cccgtcataa tgaacttatt
     2521 tatttggaaa aaggaatttt tctagcggat acaccaggat ttcagaatct aacccttaca
     2581 tctatggatg tgactgaact gcctgagtat tatcccgaat ttcaaaaagt aaattcctgt
     2641 cgctatgata attgtcttca tgataaagag ccagcttgcg gtgtaaaaaa agctgtcttg
     2701 gagaaggtta ttgtggaaag tcgttataaa aattatttgc ttttattaga cgagctccga
     2761 agtagaaagg attactaatg attattgctc cctcgattct atcagctgac ttctcccggt
     2821 tgggagagga aatcctagcc cttgaaaagg ctggtgccga ttggattcat tttgatgtta
     2881 tggatggcca atttgttcca aatatatctt ttggttcaaa aatactagaa gacataaaag
     2941 cactaacaaa tttacccttt gatgtgcatc ttatggtaaa agaaccttat aattacctgg
     3001 aagattttgt aaaaaaagga gctgattcta ttactttcca tatagaggct gtttcagatc
     3061 cctatcgcta tataaaaaaa tgtagagaat tagggataaa ggttggactt agtataagtc
     3121 cagatacagc agttgctgaa attaaccctt ttttagataa gattgacctt gtcctagtga
     3181 tgagcgttta tcctggattt ggaggacaga aatttatttc ttctagtttg gaaaaaatac
     3241 aagaattaga aaaattacga aaaacaaagg gatattcttt tctcattgaa gtcgatggtg
     3301 gagtggggcc aagcaatgca aaagaaattt cttctgcagg tgctgatatt ttagtggctg
     3361 ggacctcaat ccttgctagc aaagattata aaataagtat cgagcaacta cgatgacagt
     3421 tattattggc agtgcacccc tagaggaagc gctgcttctt tcatatttaa aagaagccag
     3481 ccgtataata gctgttgatg ggggacttaa tcatcttgaa tctgtgggac aaaaagcgga
     3541 tattgttttg ggagactttg actcttatca aggagaactt cctcatgggg ctaaggtttt
     3601 ttcaaaggag aaaaacttta cagatatgca agcagcccta gcactaataa aagaggaaga
     3661 aacggttatc ttaggagcta cgggaggaag gctggatcat tttctatcag tccttaccct
     3721 tatgcggggt agagagaaca tccagcttat agataaacaa aatcgtatat tttaccgaaa
     3781 gggaagtttt aacctaaaaa aagaagaggg atatttctcc ctctttccct tgcaagatac
     3841 gcttattacc attgaaggtg ctaaatataa tcttgataag gcccatgtgt cctcagatga
     3901 tagccttctt ttaagcaatg agtggcttgg tgatgttact ataaaaactg ataagggatg
     3961 ggtcctggtt gtactttctt tggacaaata aaaaaatccc ctgggggatt tccttacacc
     4021 gcgcggcgga ccttatttga tcgcaagcaa cgagaacaga ccatttctcg gcgtacagta
     4081 ttattatcat ctaaaatgcg tactcttcgt agatttggtt tccaaactct ttttgtatga
     4141 ttaagggcat gagagacctt atgtccactt atgttgcttt taccacacac aaaacatttt
     4201 cgtgccatgt tttccacctc ctttttaggg attcaatgta gtagcagagt cattataaca
     4261 aaacgagaat aaacaagcaa ggattttcta cttgtaagaa gcagaacaat tctttacaat
     4321 aggcaatagc agggaggtca tcatgaaaca aaaacaaata acaaaatatg gtagtattaa
     4381 aattgataaa aaagtaatag aagatttggc aagaaaggct gctagtgagt gttatggtgt
     4441 tttagcgatc gtagatgttg gatccatgga cagcgtgata caaaaactga aaaaagagac
     4501 catttctggt ggtgtaagag ccagcgaagg cgaggaaggt cttatccttg atgtttatgt
     4561 aagtttgcag tatggtgtac gtacggcagc tgttgctgaa aatattatgg aaactgtaaa
     4621 atatcaaata gaaagtactc ttgagcttcc agtagaagaa gtgaatgttt ttattcagag
     4681 tataaatgta ggtgagtgat ggaaataatg caattagatg acaaaacttt acgctctatg
     4741 cttagggcgg gatactataa actaaatgcc gataaagaga tggtaaatag tctcaacgtc
     4801 tttcctgttc ctgatggaga cacgggtact aatatgtcct tgaccttaaa gacggccatt
     4861 gaaatggtag acaaagaaga tagtaagcaa acgggagaga ttctgcgtag cttttctagg
     4921 ggttctctaa tgggagctag aggcaatagt ggagtaattc tttctcaaat atttggtggt
     4981 cttgctcgtg ggactgaaaa taccaaaacc ctaggggtaa aggattttat taaagcatta
     5041 gatgagtctg tggaagtagc gtataaggct gtaatgaaac cagtagaagg taccattttg
     5101 actgttattc gccagagcac ccagtggatt aaggaagtcg aaggtgttga attattagat
     5161 ttcgaaacat tttttgctct tcttctggaa aaggcccaac tttctttaaa gggaactcca
     5221 gatcttcttc ccatacttaa acaaagtggt gtggtagatg ccggcggtca gggcctatgt
     5281 tctattttcg agggtttttt agccagtatt cgaggagaag aagttttgtt tgcagaagag
     5341 aatgatgcta tagaacatac tgcatatcat ccagaagcaa cagatatcca ttttgcctat
     5401 tgtacagaat ttatcctaag agcccgtgag aaaagcaatg aaaaattaaa agaaaaaatt
     5461 ctcgaaatgg gtgatagcgt tgtttatgtg caggatgagg atatagtaaa aattcatctc
     5521 cacacgaata atccaggcgt cgctttagaa actgctcttg aatatggcag tcttttaaat
     5581 attaaaattg ataatatgaa gattcaacat gaaacgataa tgggtattga tcataaagaa
     5641 gaagaaaata ttattataga agaagaaata aaaaatgttg cagtaattgc agttgcagct
     5701 ggtaaaggtt ttaaggatat ctttacagat cttggcgtta gcgctgtgat tgagggtgga
     5761 caaactatga atccatcaac agaagatata atggcagaag ttgataaaat taaagccaat
     5821 catatactga tcctacctaa taattccaat ataatacttg cagctcaaca agcgcagaat
     5881 atgagtcaaa gaagcatcca tgttgtagag accaaaacaa tacctcaggg cattgcagct
     5941 atgatgggct ttgatccctt tggtgatatt gaagaaaaca ttgaatccat ggaagatttt
     6001 tttgatgttt ctacgataca aataacctat tcggtgcgca acaccaaaat ccaaggcttt
     6061 gttatcaaag aaaaagatat cctttgtata aaagatggtg agattgttgc cgttgaaaaa
     6121 actccagaaa aggctcttcg taaagtacta gagctctact cctcaaccta tgatcttatt
     6181 accatatatg ggggagaaga aagttctagt ggcagaacga aaaaaattat agatgaaatt
     6241 caaaagaaaa atgaagagat agaaatcgag ttatatcaag gtgaccagcc cgtgtattat
     6301 tatatct
//
LOCUS       DASRNA010000068         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_240170,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000068 DASRNA010000000
VERSION     DASRNA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240170"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2480)
                     /locus_tag="VFD08_01960"
     CDS             complement(<1..2480)
                     /locus_tag="VFD08_01960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HZK09813.1"
                     /translation="MLKEQEALIKKVNINPESCKVSFSRKSKRSSIICGRNILTDPIP
                     ISEVNLDLDVATIEGEIISLESRELKNGETLLVILAVTDYTSTITCKMFLRYRRNGQD
                     NNHESPSISPAEKARVEKIVNMLSEGSFVKIRGRCVYDTYADEVSLMIQDIIAVKKEE
                     RLDTAPLKRVELHMHTHMSAMDALSSPEVLIERAASWGHTAVAITDHGVLQAFPAAFS
                     AAKKHSIKLLPGLEAYMIKENIVVENCKNISLNKPIVVIDFETTGLNTKTDKIIEIGA
                     VKLDGDKIVETFSELINPHISLPSKITKITGITDAMLNGKPSIEQVMPDFLKFVGNCP
                     IAAHNASFDMGMLESELERQSITLDVAQIDTLALSRKLYPMLKSHRLKSLCRHLNVSL
                     QNAHRAFDDARATAQCLSKMIEEVERLGACTLPEIDQLIQGGVIGQRFHIILLAITQK
                     GLTNLNRLVSDAHINHFQKFPHLPRTLIEKYREGLLIGSACEAGELYQAVLNKQPDSE
                     LEKIAGFYDYLEIQPTSNNAFLIKNGMVTSEKELEEHNKKIVSLGSLLDIPVVATGDV
                     HFLDPKDAIGRSIIQSGLGYSDSDEQPPLYFKTTQEMLKDFAYLGEEKAKEVVITNTQ
                     KIAARAEDLSLFPIHPEGKSTFQPLWTDAADKIKTMSVKRAKDIYGQNLPDIVDTRLN
                     KELKSIIGNGFATLYMIAHKLALKSLQDGYVVGSRGSVGSSFVATMCDITEVNPLGPH
                     YVCTNCKYSDFDVDKKAFKVGVDLPDKLCPNCSTMLHKEGFDIPFEVFLGFKGDKVPD
                     IDLNFPSSYQPIAHTFVENLFPKGHVYRA"
BASE COUNT          755 a          490 c          461 g          873 t
ORIGIN      
        1 gctctatata catgcccttt aggaaataag ttttcaacaa aagtatgggc tataggctga
       61 tatgaacttg ggaaattcaa gtcaatatct ggaaccttat ctcctttaaa acctaaaaat
      121 acttcaaagg gaatatcaaa accttctttg tgtagcatag tactacagtt aggacaaagc
      181 ttatctggaa gatccacacc aactttaaaa gctttcttgt ccacatcaaa atcactatac
      241 ttacaattgg tgcaaacata atgggggccc aagggattaa cttcagtaat atcacacata
      301 gtagctacaa atgatgagcc aactgagccg cgagaaccca caacgtatcc gtcttgaagg
      361 gatttaagcg ctagtttatg cgctatcata tagagggtag caaatccgtt accgatgatg
      421 gatttaagtt ctttattcaa tctagtatca actatatcgg gaagattttg tccataaata
      481 tcttttgccc ttttaactga catggttttt attttatcag cggcatctgt ccaaaggggc
      541 tgaaaggtac tttttccttc aggatgtatt ggaaaaagtg aaagatcttc ggctctagca
      601 gctattttct gtgtattagt tattactact tctttagctt tttcttcgcc taaataagca
      661 aaatccttta acatctcctg agtagtctta aaataaagag gcggttgttc atcgctatca
      721 ctgtagccta atccagattg tataatggat cttccgattg catctttagg gtctaaaaaa
      781 tggacatctc cggttgcgac cacaggaata tcaagaagac ttcccaggga aacaattttt
      841 ttattatgtt cttcaagctc tttctctgaa gttaccatac cattttttat taaaaaagcg
      901 ttgtttgatg taggttgaat ttcaagataa tcatagaaac cagctatttt ctctaattcc
      961 gaatcaggct gtttgtttaa aacagcttga tataattcgc ctgcctcaca agcagaacct
     1021 atcaacagtc cctcgcgata tttttctatt agtgttcttg gcaaatgggg gaatttttga
     1081 aaatgattta tgtgagcatc tgaaaccaaa cgatttagat tagtcagtcc tttttgcgtg
     1141 atagctaata ggattatatg gaaacgttga cctataactc cgccttgtat tagctggtca
     1201 atttctggca aagtacaggc acctagtctc tccacctctt caatcatctt ggaaaggcat
     1261 tgagctgttg ctctagcatc gtcaaaggct ctgtgagcat tttgcaaaga tacgtttaga
     1321 tgcctacaca aagacttgag tctatggctt ttaagcatag gatataattt tcttgaaaga
     1381 gctagggtat ctatctgggc aacatcaagt gtgatggact gtctttctaa ttcgctttct
     1441 agcattccca tatcaaaaga tgcattgtgg gcagcgatgg ggcaatttcc aacaaattta
     1501 agaaaatctg gcataacttg ttcaatagag ggttttccat tcagcatagc atcagttatt
     1561 ccagtaattt ttgtaatttt gcttggtaga gaaatatgag gattaattaa ttcagaaaaa
     1621 gtttctacaa tcttgtcacc atcaagtttc acagcaccga tttcaattat tttatcagtt
     1681 ttagtgttta agcctgtagt ctcaaagtct ataactacta tgggcttgtt tagtgaaata
     1741 tttttacaat tttctacaac tatattttct ttaatcatat aggcttccaa accaggcaat
     1801 agctttattg aatgcttttt agctgcagaa aaagcagcag gaaaggcttg taggaccccg
     1861 tgatcagtta ttgccacagc agtatgcccc cagctggctg ctctttcaat aagcacttca
     1921 ggggatgata gggcatccat tgcgctcata tgagtgtgca tatgtagttc tactcgttta
     1981 agtggtgcag tatctaggcg ctcttctttt ttaacggcta taatatcttg tatcattaaa
     2041 ctaacttcat cagcgtaagt gtcataaaca catctaccac gaattttaac aaaggagcct
     2101 tcagaaagca tattaacaat tttttctacc cttgcttttt cagcaggact tatagaagga
     2161 ctttcatgat tattgtcttg cccatttctt ctataccgta aaaacatctt acatgttatt
     2221 gtggaagtat agtcggtgac agccaaaatt actagtaggg tttctccgtt tttcagctct
     2281 ctactttcta agcttataat ttcaccctct attgtagcaa catctaaatc aagatttact
     2341 tctgatatgg gaatagggtc tgttagaatg ttgcgtccac atataatgga cgagcgctta
     2401 gattttcttg aaaaagagac cttgcagctt tcaggattaa tatttacttt ttttatcaaa
     2461 gcttcttgct ctttaagcaa agaatcttcg cgttcttttt ttaaggcagc tatcctagat
     2521 tcctggttac cttttatcct aagttcaatt gggacttcta tagaaaatat gttccatat
//
LOCUS       DASRNA010000069         4760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_323771,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000069 DASRNA010000000
VERSION     DASRNA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323771"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VFD08_01965"
     CDS             complement(<1..1160)
                     /locus_tag="VFD08_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZK09814.1"
                     /translation="MLEHYKEFLTKKRGRYLLGIFLLLLVDTIQLIVPQILRSYTNKL
                     QANTLASVEIWKIALAIGALAILMAILRYLWRVMIVFTALEFEIWTRTKLYNHWTTLP
                     MEYFNQVKTGELMSYATNDINTIRRTFSSGIIMGVDALFMTVTTIIVMGTTINWRLTF
                     FALLPMPFIVYGVLRMGKIVHKKFRTVQEIFSQLSDKVQESFSGISVIKAFSQEELDL
                     EDFDEINQRNFMENMSLARFQARLFPTVRFLGRGSMLIGIILGAKYVLAGTINLGDFV
                     AFIKYLEILVWPVMAIGMVTNLIQRGLASMQRVNDVLDIESSLEETDNPQINEGYDLE
                     VKNLNFSYPGVDNEVLSDISFSLPQGGSLGILGPTGSGKSTVASLLLRLYNIP"
     gene            1308..2777
                     /gene="guaB"
                     /locus_tag="VFD08_01970"
     CDS             1308..2777
                     /gene="guaB"
                     /locus_tag="VFD08_01970"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZK09815.1"
                     /translation="MLVREGLTYDDVLLVPGESHVTPDKVSLATRLTKKITLQMPLIS
                     AGMDTVTESQMAVAMARSGGIGIIHKNMSVEKQALEVDRVKRSEHGVITDPFYLSPHH
                     LVKDALELMSRYRISGVPIVDKEKTLKGILTNRDVRFIKDMTQPIAEVMTKENLVTAR
                     EGTSLEEAEAILRKHKIEKLLLVDDNYKLKGLITIKDIEKVVIFPNRATDSQGRLLAG
                     AAIGIGSDMKERVESLLKSQVDVLVLDSAHGHSKNVVEALKWIKENYDIEVIAGNIAT
                     KKAAKALIDAGADALKVGIGPGSICTTRVVAGIGVPQLTAIMDVADYALEAGVPVIAD
                     GGVKYSGDIVKALAAGASTVMLGSLLAGTDESPGERVIFKGRSFKSYRGMGSLGAMKS
                     GSSDRYFQNESKKFVPEGVEGRVPYRGFVKDTLYQMNGGLRSGMGYVGAKDLVELREK
                     AEFIKISNAGLIESHPHDIQITAEPPNYSDSRDNYDNNM"
     gene            2761..4251
                     /gene="guaA"
                     /locus_tag="VFD08_01975"
     CDS             2761..4251
                     /gene="guaA"
                     /locus_tag="VFD08_01975"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZK09816.1"
                     /translation="MIIICDFGGGLAQSLARQVRELGVYSEIMPYFKEDEMIKKEAKA
                     LILIGDDKKALELSHEKSMKKIYEMGLPILALGYGTPRMVMDLGGKIKEVAEVEKMQR
                     ILFSDNSPFLLSSDLSLCIDGGYLFKELPPGFENIAFSEDVPQVAIGSSKKNQYGFLI
                     KHATEDILKTFLFDIAKIEKSWNLKDFAYRQIAKIRQQVGDKKVLCALSGGVDSSVAA
                     LIVHKAIGDQLTCIFVDHGLLRKGERQEVMENYGKSFSMDIRCVEAEKRFLGKLEGIS
                     DPEKKRKIIGEEFIRVFEEEANKIGDIDFLVQGTIYPDVVESGEGASANIKSHHNVGG
                     LPERMDLKLIEPLRELFKDEVREVGEILGLEHHFVWRQPFPGPGLAVRCLGAITKKRL
                     DILSEADAIFREEIARAGLEEKIWQYFAVLPGISSVGVKDQARTYFETIALRAVHSVD
                     GTSSDWARIPYETLEIVSRRIVSEVPGVNRVVYDITTKPPSTIEWE"
     gene            complement(4431..>4760)
                     /locus_tag="VFD08_01980"
     CDS             complement(4431..>4760)
                     /locus_tag="VFD08_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HZK09817.1"
                     /translation="LIHNKLTKAQIGITYADEADLLNVALFGSTAKEWKINNPDKDGN
                     MRDYATIEELIVMANLESANAQFIAENVSQAERLKKLNQMARFQLKSLLNNSSLKKLE
                     GNKSIEK"
BASE COUNT         1505 a          854 c         1084 g         1317 t
ORIGIN      
        1 gggatattgt atagacgaag aagaagacta gcaactgttg atttaccaga gcctgttggt
       61 cctaaaatgc caagggatcc cccttgggga agggaaaaag aaatgtcgct aaggacctca
      121 ttatccacac cggggtagct aaaattaagg tttttaactt ccaagtcata gccctcattt
      181 atttggggat tatctgtttc ttctaaggaa gactctatat ctaaaacatc gttgactctt
      241 tgcatagagg caaggcctct ttgaataaga tttgttacca taccaatggc cataacaggc
      301 cagacaagaa tttctagata tttaataaag gccacaaaat cacctaggtt aatggttcca
      361 gctagaacat attttgctcc aagaataata ccaataagca tagaaccccg gccaagaaag
      421 cgtacggtgg gaaatagccg ggcttgaaat cttgccaggg acatattttc cataaaattc
      481 cgctggttaa tttcatcgaa atcttctaga tcgagttctt cctggctaaa ggccttaata
      541 acagaaatac cagaaaaaga ctcctgcacc ttatctgaaa gctgggagaa gatttcctgt
      601 acggtacgga actttttatg aacgatttta cccattcgaa gaactccata tacaataaag
      661 ggcatgggca agagggcaaa aaaggtgagt ctccagttga tggtagttcc cattacaata
      721 atggtagtga cggtcataaa caaggcatcc acgcccataa tgattccgct actaaaggtt
      781 cgccttatgg tgttaatatc attggtagcg tagctcatta gctcgccagt ttttacttgg
      841 ttaaagtatt ccataggcag ggtggtccag tggttgtata gcttggttct ggtccatatt
      901 tcaaattcta gggcggtaaa gacaatcatc acccgccata aataccgcaa aatagccata
      961 agaatggcta gggccccaat agcaagagcg atcttccaaa tttctacaga ggccaaggtg
     1021 ttggcctgga gtttattggt atagcttctt aggatttggg ggacaataag ctggatggta
     1081 tctaccagta aaagcaaaaa gatgcccagt aaataccgac ctcttttctt tgttaaaaat
     1141 tctttatagt gttctagcat aattgcctcc taaaatatca ttttatcata atagccagaa
     1201 ttaacctata cttagaagca aaagaccagt atactataga tagaaagtac gaacttataa
     1261 aagtgttcgt caatagtagt gtttgccaat tttttaagga ggagataatg cttgtacgag
     1321 aaggtcttac ctatgatgat gtactcttgg ttccaggaga aagccatgtg acgccggata
     1381 aggtttccct ggcaacccga ctgacaaaaa aaataactct tcaaatgccc ttgattagtg
     1441 caggaatgga tacagtaaca gaatctcaga tggctgttgc catggcgcga agtggtggca
     1501 ttggaattat tcataaaaat atgtctgtgg aaaaacaagc cttagaagta gaccgggtta
     1561 aaagaagtga gcacggtgta attaccgatc ctttctatct gtccccccat catcttgtca
     1621 aggatgccct ggagcttatg agccgctatc gcatctctgg agttcctatt gtagacaagg
     1681 aaaaaacact gaagggaatt ctgacaaata gggatgttcg ctttattaag gatatgaccc
     1741 agcctatagc tgaggttatg accaaggaaa atcttgttac ggctagggaa ggcacaagcc
     1801 tggaagaggc agaagcaatc cttagaaaac acaaaataga aaagctgctt ttagtggatg
     1861 ataactataa gctaaaggga ctgattacca ttaaagatat tgagaaggtg gttatctttc
     1921 ccaatcgtgc cacggacagc cagggtagac tcttagcagg ggctgctatt ggaattggaa
     1981 gtgatatgaa agaaagagtg gaaagcctgc taaagtctca agttgatgtt cttgttttgg
     2041 actcggccca cggtcattca aaaaatgtag tagaggccct aaaatggatc aaagaaaatt
     2101 atgatataga ggtaatagcg ggcaatattg caacaaaaaa agcagccaaa gcccttattg
     2161 atgcgggagc ggacgctctt aaggttggaa ttggtccagg aagtatttgt acaacaagag
     2221 tggttgctgg aattggggtt ccacagctta ccgccattat ggatgtagca gactacgcgc
     2281 tagaagctgg agtaccggtg attgctgatg ggggcgttaa gtattcagga gatattgtaa
     2341 aagccctagc ggcaggagcc agtacggtta tgttaggcag cctccttgcg gggaccgatg
     2401 aaagcccagg agaacgggta attttcaagg gaagaagctt taaatcctat cgaggaatgg
     2461 gaagtcttgg tgctatgaaa tcgggaagta gcgatcgcta tttccaaaat gaaagcaaga
     2521 aatttgttcc cgagggagtc gaaggaagag ttccctaccg gggatttgtt aaagatactc
     2581 tttatcaaat gaacggggga cttcgctcgg gcatgggata tgttggagcc aaggacctgg
     2641 ttgaacttag agaaaaagca gagtttatca aaatctctaa tgcaggactc attgagagtc
     2701 atccccatga cattcagatt actgccgaac caccgaacta ctctgattcg agggacaact
     2761 atgataataa tatgtgattt tgggggagga cttgctcagt ctctggccag acaagttcgt
     2821 gagcttggcg tatattcaga gattatgccc tattttaaag aagatgagat gataaaaaaa
     2881 gaggccaagg cccttatcct gataggagat gataaaaagg ccttggagct tagccatgaa
     2941 aagtccatga aaaaaatata tgagatgggt cttcccattc ttgctcttgg ctatggaact
     3001 cctcgtatgg ttatggatct tggaggaaag atcaaagaag tagcagaagt agaaaagatg
     3061 cagaggattc tcttttctga taattctccc ttcttactaa gtagtgattt aagcctttgt
     3121 attgatggag gctatctctt taaagaacta cctccgggtt ttgaaaatat tgctttttct
     3181 gaagatgtgc cccaggtagc cattggaagt tctaagaaaa accagtatgg ttttttaata
     3241 aaacatgcga cagaggatat attaaaaacc ttcctctttg atattgctaa gatagaaaag
     3301 tcctggaatc ttaaagactt tgcctataga caaattgcaa aaattcgtca gcaagtagga
     3361 gataaaaaag tcctctgtgc ccttagtggg ggggttgatt catctgtagc agcccttatt
     3421 gtgcataagg ccattggaga tcagctgacc tgtatatttg tagaccatgg ccttcttcgc
     3481 aagggcgaaa gacaagaagt aatggaaaac tatggaaaga gtttctccat ggatattcgt
     3541 tgtgttgagg cagaaaagcg ctttctagga aaactagaag gcataagtga tccagagaag
     3601 aaaagaaaga ttatcggaga agaatttatt cgtgtatttg aagaagaagc aaataaaata
     3661 ggcgatatag attttcttgt acagggaacg atttatccag atgtagtaga aagcggtgag
     3721 ggagcatcgg caaatataaa gagccaccat aatgtgggcg gccttcccga aagaatggac
     3781 cttaaactga ttgaacccct acgagagctt tttaaagatg aagtaagaga ggtaggagaa
     3841 atccttggcc ttgaacacca ttttgtatgg cgtcaacctt ttcccggccc cggtcttgca
     3901 gtacgctgtc taggagctat aaccaagaag aggctagata tcctaagtga agccgatgcc
     3961 atctttagag aagaaatagc tagggcaggc ttggaggaaa agatttggca atactttgct
     4021 gttcttcctg gaatttcttc tgttggagtc aaagatcaag ccagaaccta ctttgaaaca
     4081 atagccctta gggctgttca ctctgttgat ggaacaagtt cagattgggc caggataccc
     4141 tatgagacct tagaaattgt ctcccggcgt attgttagtg aggtgcctgg agtaaaccga
     4201 gtagtctatg atattactac gaaacctcct agtactattg agtgggagta gttaaaaaca
     4261 aatataaaag ctgattaaac caatacttgt agaggttaaa aaccctcgac tggaacgaaa
     4321 ttgggtaaaa aagttttaaa gaataactta ataaataatg ccaactggct tacaagtaga
     4381 aacaccatag agatcttata aaaagaatct atggtgtttt gtgtgtatat tcatttttca
     4441 atagatttat tcccttctaa tttctttaaa gaggagttat ttaatagaga tttcaattgg
     4501 aatctagcca tttgatttaa tttttttagc ctttcagctt gtgatacatt ctctgcaatg
     4561 aattgagcat tagcagactc aagatttgcc attactatta attcctctat agtagcataa
     4621 tccctcatgt ttccatcttt gtctgggttg tttatcttcc attccttagc agtagatcca
     4681 aataaagcaa cattcaataa atctgcttca tctgcataag ttatacctat ttgtgcttta
     4741 gttaatttat tatggattaa
//
LOCUS       DASRNA010000070         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_324056,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000070 DASRNA010000000
VERSION     DASRNA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324056"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFD08_01985"
     CDS             <1..604
                     /locus_tag="VFD08_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FctA domain-containing protein"
                     /protein_id="HZK09818.1"
                     /translation="IYDNSEYKVIVDVSKKADGDYEAKTSYEKENKLIDGVPIFKNTS
                     SAKAALIKLQAEKTLDDKAPKDGRFSFILTDEKGQVLQRKSNKEGKILFDEFPIKEEG
                     SKIFYLKEEVGTDKDIIYDKSEYKVIVVTKKDKDGNYEASITYKKAGKTFEGSPIFKN
                     KIKVKTLPSTGQAKSSIPLLGGGLLLVGIALTFSQKKKEN"
     gene            768..1595
                     /locus_tag="VFD08_01990"
     CDS             768..1595
                     /locus_tag="VFD08_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK09819.1"
                     /translation="MQEKIIVHYPQDEMTTFLLPITQGCSYNKCKFCTMYKDQRYKEV
                     SLSEIELILKSGHLYTEKVFLVGADPLSLGFEKTLHILGLIKKHLHYCACVASYASIR
                     NIARYSEKELTILHNAGLRLLYIGFETGRDDILKKMNKPHSLTEALREAKKLNRAHLP
                     FNTIVLSGIAGSAQGIKNAEATAQMINKFTTNKVITMKLMVFHGSLLAEEVKKGDFIP
                     AAKEELLLELEHLLTHLQVKKPMIFDTTHPSNIIKIKGTLPQDKRRLINKIRQAKDK"
     gene            1692..>2269
                     /locus_tag="VFD08_01995"
     CDS             1692..>2269
                     /locus_tag="VFD08_01995"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013310222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HZK09820.1"
                     /translation="MEEGPGLENLSPQAIKKIRDFHKSFKEYKVTPLHRLRELSDHYG
                     VKEILVKDESHRFGLKAFKVLGGSYAIGKDLAGRLDMDIADLSFEYLKREDIRKKLGE
                     ITFVTATDGNHGRGVAWAAKKLGQKSVVYMPKGSSQIRLDAIRREGAQAEIIEGNYDD
                     AVRLSNQMAKKYGWVVLQDTAWPGYEKIPTWI"
BASE COUNT          835 a          418 c          470 g          546 t
ORIGIN      
        1 catctatgat aattcagagt ataaggttat agtagatgtt agtaaaaaag cagatggaga
       61 ttatgaagcc aagacgtctt atgaaaaaga aaacaaactt atagatggag tcccgatttt
      121 caaaaacaca agcagcgcta aggcggcact aatcaaattg caagcagaaa aaacactaga
      181 tgataaggcc ccaaaagatg gacgctttag ctttatccta accgatgaaa aagggcaagt
      241 ccttcaaaga aaatcaaata aagagggaaa aatcctattt gatgaatttc caataaaaga
      301 agaaggaagc aagatcttct atcttaagga agaagtaggg actgataaag acatcatcta
      361 tgataagtca gagtataaag taatcgtggt cacaaaaaaa gacaaagatg gaaactatga
      421 agccagcatt acctataaga aagctggtaa gacctttgaa ggaagcccga tatttaaaaa
      481 caagatcaag gtaaaaacac ttcctagtac aggtcaagca aaatcatcaa ttccgctttt
      541 gggaggtggg ctacttctag taggaatagc attgaccttt tcacaaaaga aaaaagaaaa
      601 ttgataaggc aatcagagag tcctcttggg ctctcttttt ttttaggaga tagtcttatt
      661 tttagcaagg ctagccctat aggaaaactt taagcaaggc ttattttgca gagaaatctt
      721 aatggagtat aataaagata atcacttgct ccctaggagg tttatttatg caagaaaaga
      781 ttatcgttca ttatccccaa gacgaaatga cgacatttct tctaccaatc acccagggtt
      841 gttcttataa taagtgcaaa ttttgtacca tgtataagga ccaaaggtat aaagaggttt
      901 ctctctcaga gatagaactg attttaaaaa gtggtcatct ctatacagaa aaagtttttt
      961 tagtcggagc tgacccctta tcccttggct ttgagaagac cctacatata cttggactaa
     1021 ttaaaaaaca tcttcattac tgtgcttgtg tggcctccta tgcatccata agaaatattg
     1081 caagatatag tgagaaggaa cttaccatcc tccataacgc aggacttaga ctactctata
     1141 taggctttga aacaggcaga gatgatattt taaagaaaat gaataaacca cacagcctaa
     1201 cagaagcact aagagaggcc aaaaagctca atagagcaca tttgcccttt aacactattg
     1261 ttctaagtgg gatagcaggc agcgctcaag gaataaaaaa tgctgaggct acagcccaga
     1321 tgataaacaa gttcactaca aataaggtta taaccatgaa gcttatggta tttcatggat
     1381 cccttcttgc ggaagaggta aaaaaaggag actttattcc agctgccaag gaggagctcc
     1441 tcctagagct agagcatctt cttacacatc ttcaggtaaa aaaacctatg atatttgata
     1501 ccacccatcc aagcaacata ataaagataa aggggacctt gccccaggac aaaaggcgcc
     1561 taatcaacaa gattaggcaa gcaaaagata aataatcaag ccacctacta taaaaagagc
     1621 aaagataaaa ggaggaatgt tctttggaag aaaataaaaa ctacattaaa taccatctag
     1681 tggataaaaa aatagaagaa ggcccgggcc tagagaacct atccccccag gccataaaaa
     1741 agattcggga ctttcataag agctttaagg agtataaagt taccccccta cataggctaa
     1801 gggagctttc agaccactat ggagtaaagg agatcctggt taaggatgag tcccaccgtt
     1861 ttggactaaa ggcctttaaa gtcttaggcg gctcctacgc aatcggaaaa gaccttgccg
     1921 ggagactgga tatggatata gcagatctct cctttgagta tttaaagagg gaagatataa
     1981 gaaaaaagct aggagaaatc acctttgtta ctgccaccga tggaaatcat ggaaggggag
     2041 tcgcctgggc tgctaagaaa ctaggccaaa aatccgtcgt ctatatgccc aagggatctt
     2101 cccagattag gctggatgcc ataagaagag aaggagccca ggcagaaatc attgagggaa
     2161 actatgatga tgcggttaga ctctcaaatc aaatggctaa aaagtacggc tgggtggttt
     2221 tacaagatac agcctggcca ggctatgaaa agatccccac ctggattat
//
LOCUS       DASRNA010000071        11175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1068361,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000071 DASRNA010000000
VERSION     DASRNA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068361"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFD08_02000"
     CDS             complement(<1..580)
                     /locus_tag="VFD08_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HZK09821.1"
                     /translation="MRGIGTVINIIGVLFGGSLGLIMRRGIPENLRSQLIRVQGLSVV
                     VLGLSGVLSRIGSYENDVILMLLAMVFGTLLGGLIHIEGAIEAFGERLKGRYGSKEEG
                     FVQGFIIASMTMCIGAMGIIGAFEDALFNDISILLAKTVLDGFTAFILANYYGRGVLF
                     SILPLALWQGSFTLLAGFLAPILTDSVLHGTSI"
     gene            complement(636..1589)
                     /locus_tag="VFD08_02005"
     CDS             complement(636..1589)
                     /locus_tag="VFD08_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK09822.1"
                     /translation="MKLIREFEVGERIEGFYLIKEFNLRTSSNNNKYLDLTLEDVSGQ
                     INSKIWNIGEGEEDLYQSGDIIKVRGDITLWMDKMQLKIIKHRPEEEGDEVDHKLLIS
                     SAPYEGLAMWEELFTRAQKIEKESLRKMAIAALEVHKEKMLYYPAAKSNHHSIRGGLL
                     YHLLRMYKAGEMLSELYELDADLVFAGVIYHDLEKITEMSANEMGIVSTYTKEGTLLG
                     HIISGIGRIEKIAEEVGCDEETKLLLQHMVLTHHYEPEYGSPKKPMFPEAELLHYLDI
                     IDARIYDMQKAVSNIEEGSFSSPVFSLGRRTVYKPLESEEE"
     gene            complement(1612..2346)
                     /locus_tag="VFD08_02010"
     CDS             complement(1612..2346)
                     /locus_tag="VFD08_02010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZK09823.1"
                     /translation="MNEVLAKVGNKSLTREDLRDFQASMPPEYAQQLASLGEEVIVEE
                     LINQELLYQDALANKIQDSEAYKAEMDKLSKEVLKGMAINEVLMKAEPSQDESESFYN
                     ENKESFVRPEQVAASHILVENLEEATAIKKQLDEGGDFSQLALEKSTCPSKENGGALG
                     LFEKGKMVPEFEEVAFKMQVGETSQPVKTQFGYHIIQVDNKTSEGRKTFEEALPEIVE
                     QLRSQKQQEAYAGYMEELRKKFPVTK"
     gene            2696..3688
                     /gene="trpS"
                     /locus_tag="VFD08_02015"
     CDS             2696..3688
                     /gene="trpS"
                     /locus_tag="VFD08_02015"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZK09824.1"
                     /translation="MKKRIFSGIQPSGDLTLGNYLGALRNWPRLQEDYHAIYSIVDLH
                     AITVKQDPKLLKERTLLTLALYLAAGIDPEKSILFIQSHVPQHAELNWLLACHTYMGE
                     LSRMTQFKDKSSRLSQKDSIPSGLFNYPTLMAADILLYQAQLVPVGEDQSQHLELTRD
                     LAIRFNNAYGKTFAIPKGYFNPFGAKIYDLQNPTATMSKSSVNQNGVLFLMEEEKSLR
                     KKIARSVTDNLAVIRYSDEQAGVKNLLNIYSLLSGKTPDKTLLHFEGKGYKALKDDVS
                     ELVLDELLPLQKRTLEYLGDTVELEKIYSKGAKKAKELAKPTLEDVKEKLGFVI"
     gene            3705..5165
                     /locus_tag="VFD08_02020"
     CDS             3705..5165
                     /locus_tag="VFD08_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007242474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HZK09825.1"
                     /translation="MNIAIKDGQFISLDESMANRHGLVAGATGTGKTITIKLMAEEFA
                     KAGVPVFITDIKGDLSGFLEAGTLSPKLAERLEKIGAPSPEFESFTVNFWDVLKENGI
                     PLRATISEMGPLLLSRLLNLNDTQTGVLYALFKIADDQGLLLLDFKDLSEMLKFLYEN
                     SKELSKDYGNMSKASIGAIQRSLLILEQQGATEFFQEPALDVRDFLQKDRQARGVINI
                     LDARKIYHNPDIYSAFLLLLLSNLYEELPEVGDQPLPKLVFFFEEAHLIFQNASKVLL
                     EKIEQIVKLIRSKGVGVFFITQNPRDIPDNILAQMGNRVQHALRAYTPAEQRLVKAVA
                     DSFRNDAEMNVAEELLALKTGEALISTLDENGAPTPVVKAFILAPASNMDPASSQSVE
                     NAIKNSYFYNKYEKTFDSHSAYEVLKERAEQMAEEVKSSDKKEKEEGLLGSLLKPRSS
                     RDTAMERMIKNTMGSFGSQLGREIFRGVLGGIKKDK"
     gene            5259..5696
                     /locus_tag="VFD08_02025"
     CDS             5259..5696
                     /locus_tag="VFD08_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZK09826.1"
                     /translation="MTKNKAFPWILVLLWMGLIFYFSHQPASQSAQLSRGLTVTIINI
                     IEKLAPNIDFDFGAFHHFIRKNAHFFAYLILGLLVANAMRVRKTKRALAAIILCTLYA
                     LSDEFHQTFIPARAGQWTDVLIDSMGSAFGVFSYQIALFMKKR"
     gene            complement(5768..6265)
                     /locus_tag="VFD08_02030"
     CDS             complement(5768..6265)
                     /locus_tag="VFD08_02030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK09827.1"
                     /translation="MKNISMRKYEKKDCDRVLEINKELVHFLSPLTKEKLNSLIDQSE
                     MFNVVEIDGLVEAFVLTFREGKKYDSINYLWFSEHYERFLYIDRVVVSSKMHGKKLGK
                     LLYESVFIHARQTGVDCVAAEIDIEPPNPGSLLFHKKFGFKEVGQQKVAGGKKLVSLQ
                     VASSN"
     gene            complement(6378..7283)
                     /locus_tag="VFD08_02035"
     CDS             complement(6378..7283)
                     /locus_tag="VFD08_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK09828.1"
                     /translation="MKYKGEIALLGVTLFWGFGYSITEYALNMGVSSLQIQALRFLIG
                     FLCLLPLSLKRRGDLTRQTVKKGIILGLLMFSFFYLIHEGQRLTNTSKTAFLVGSYVL
                     FVPAFDWIYNKKIPEGRTFVAAVLSLFGIYLISSGNFGNLTRGDYLLLGGALLVALHM
                     VLSAEYVKDERPIHLNFVQIGVTALFSLIGALLFKSFAPIEAKPLLAIIYIGVVSTFI
                     AYMLQTTGQKYTTATRASILLSLEAPVGTFFGIIIHKDPINFSMSMGYLCIFIAILVS
                     EGIFMKLFRNWHFRRKLVGQGGKRL"
     gene            complement(7273..7863)
                     /locus_tag="VFD08_02040"
     CDS             complement(7273..7863)
                     /locus_tag="VFD08_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZK09829.1"
                     /translation="MEHYFSEKTKIPSDPKRITLYFNKKSYPFITDRGVFSKDRLDFG
                     TDLLIKTSLDRISGEVLDLGCGYGPVGTLVYLHLGIVCHMSDVNQRALELAKKNLELN
                     KAKGKVFYSDGFSKIDKKFDVILFNPPIRRGKETIYFLFKEARQHLYPGGRLMIVIQK
                     KQGMKSAKKRLEELFANVVVLDKKAGYYILEARREV"
     gene            complement(7847..8428)
                     /locus_tag="VFD08_02045"
     CDS             complement(7847..8428)
                     /locus_tag="VFD08_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09830.1"
                     /translation="MELKNFPLVQGSYGGNQAWFKKSLWAAGGCGVIASANIYYYFLE
                     KKNVDKKEYLELATALYYWLKPLHFFNPHSKEDTYGMISPGLWAQRTVRFCRIRGIFL
                     ETKVFRFIRKRALLEAKKALLKGELPAIMVLGPPASTPYVNHFMVITGFSGDKLIFST
                     WGRKVEIDFSELAAPGIFFHLIIFKGGEDGALF"
     gene            complement(8553..9533)
                     /gene="tsaD"
                     /locus_tag="VFD08_02050"
     CDS             complement(8553..9533)
                     /gene="tsaD"
                     /locus_tag="VFD08_02050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZK09831.1"
                     /translation="MLILGIETSCDETAAALVEDGRKVLSSVIHSQIEIHQPFGGVVP
                     EIASRSHVERIDGVVSRALEESGKRLDEIDGVAVTQGPGLIGPLLVGLNYAKGLSYAL
                     NKPLYAIHHLDGHVAANYLAFHDLEPPFLSLILSGGHSHFYLTQDYGVYKYLGGTRDD
                     ALGEAFDKVARILGLSYPGGPKVEEIARQGQPSFSLPKTMLEKGSLDFSFSGIKSAVK
                     NLADKLELTEKQKADLAASFQETVFSIVYDKLIEAIEQTGQRKIIIAGGVASNQRLRE
                     VLADLPAQVFIPPLSICTDNGAMIAGAAYYKALKEDFAPLSLNAKANLSL"
     gene            complement(9514..10164)
                     /gene="tsaB"
                     /locus_tag="VFD08_02055"
     CDS             complement(9514..10164)
                     /gene="tsaB"
                     /locus_tag="VFD08_02055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZK09832.1"
                     /translation="MQEEKILAFDTVSSVCSLCIYDGDFHNFETTSNFGHASELMPLM
                     DRALTELGYGLEDMDTLVLGLGPGSFTGIRIGLSTALGLARGAGLACYGVNSLRSRSY
                     GHKADIVVPLMDARRGNVYGAAYGAYKKEPFNGEFAVFLQSLKERDEELVFVGEKLDA
                     FKEQAPSFSFKDPGPLAQGVIQAYLDGQAQEELVPIYLREVEAKRRLEEKNANSGN"
     gene            complement(10136..10552)
                     /gene="tsaE"
                     /locus_tag="VFD08_02060"
     CDS             complement(10136..10552)
                     /gene="tsaE"
                     /locus_tag="VFD08_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZK09833.1"
                     /translation="MLIHSVEELTSYANILAKKEIPILLLIGDLGAGKTTFTKAYGRA
                     LGIENIKSPTFSIVEEHPYKGGTLFHMDLYRIGDIEELLALGFEEYFDRPGRIVIEWP
                     QVGLELIPKEAYCLEIIEEKDQVRRVKFARREDFGI"
     gene            complement(10562..>11175)
                     /locus_tag="VFD08_02065"
     CDS             complement(10562..>11175)
                     /locus_tag="VFD08_02065"
                     /inference="COORDINATES: protein motif:HMM:NF017655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HZK09834.1"
                     /translation="PIFAWALAQGFRYSSNRRAYFKRLVVFAFISQLPYSFLNPGAVF
                     TLYKLNQLFQFALSFILLNLYEKRKKSKWFYLLAMGLLLLPDLIQYFFPPFSLGYGSY
                     GLMLSLLFYLIRDPFEQILGYVLISIYGSISSGLIYGTSFSVIIQGFAGLDGFIFQWR
                     SILALPLIQISERLPKKRVPKYLFYFFYPVHIAILVIIYKVFY"
BASE COUNT         3319 a         2516 c         2024 g         3316 t
ORIGIN      
        1 caatggatgt tccatggagg actgaatctg ttaaaatagg tgccaaaaaa cctgctagta
       61 gggtaaagct cccctgccaa agagccaggg gcaaaataga aaataagacc cctcttccat
      121 agtaattagc caaaataaaa gcagtaaaac catccagcac cgtcttggct aataaaatag
      181 atatatcatt aaaaagagca tcttcaaagg caccaataat acccatggct cctatacaca
      241 tagtcataga agcaataata aagccttgaa caaaaccttc ctctttagag ccatagcggc
      301 ctttaagcct ttctccaaag gcttcaatgg ccccttcaat atgaattagc cctcctagca
      361 gagttccaaa aaccatggct agcagcatta aaatcacatc attttcatag gagccaatcc
      421 ggcttaagac ccccgatagc ccaagaacaa caacacttag gccctgtacc ctaataagct
      481 ggctgcgcag attctcggga attccccgcc gcataataag tcccaggctt cctccaaaga
      541 gcactcctat tatattaatt actgttccaa ttcccctcat tgcctacctc taatataaat
      601 acaaatagaa aataagaagg gcaagccctt cttatttatt cttcttcaga ttccaatggt
      661 ttataaactg ttcgtctacc taaactaaag acggggctac taaaactgcc ttcttcaata
      721 tttgatacag ccttttgcat atcataaatg cgagcatcta taatatctag gtagtggagc
      781 agttccgctt caggaaacat tggttttttg ggtgaaccat attctggttc atagtggtgg
      841 gtaaggacca tatgttgcag aagaagtttt gtttcttcat cgcaaccaac ctcttcagca
      901 atcttttcaa ttcgtccaat tcctgaaata atgtgaccta ggagggtccc ttcctttgta
      961 taagtagaga cgattcccat ttcatttgcg ctcatctcag tgattttttc caggtcatga
     1021 taaataaccc ctgcgaaaac aagatctgca tctaattcgt atagttcaga gagcatttct
     1081 cctgccttat acatccttaa aagatgataa aggagtcctc cacgtataga atggtgattg
     1141 cttttggcag caggatagta aagcatcttt tctttatgaa cttctaaggc tgctatagcc
     1201 atctttctta aagattcttt ttctatcttt tgcgccctag taaaaagttc ttcccacatg
     1261 gccaggcctt catagggagc tgaactgatc aaaagtttat ggtctacctc atcaccctcc
     1321 tcctctggcc tgtgctttat aatctttagc tgcattttat ccatccagag ggttatatct
     1381 cctctaacct taatgatatc accactttga taaaggtctt cttccccctc cccaatattc
     1441 caaatcttgg aattaatctg acccgagaca tcctccaagg tcagatccaa gtatttatta
     1501 ttattggagc ttgtccgtag attaaattct tttatcagat agaagccttc aattctttct
     1561 cctacctcaa attctcgaat cagtttcatt cttcctcctc cggttcaaaa attattttgt
     1621 tacggggaat tttttgcgaa gttcttccat atagccagca taagcttctt gctgcttttg
     1681 gcttcttagt tgctctacaa tttctggaag ggcttcttca aaggtttttc ttccttcact
     1741 ggtcttatta tcaacttgga tgatatgata gccaaactgc gtttttactg gctgggatgt
     1801 ttctcccact tgcatcttaa aggctacttc ttcgaattca ggaaccattt tccccttttc
     1861 aaaaaggcct agtgctcctc cattttcttt tgaaggacat gtagactttt ctagggccag
     1921 ctggctaaag tctccccctt catcaagttg ctttttaatg gcagttgctt cttcaagatt
     1981 ttctaccaaa atatggcttg cagctacttg ctcaggtcga acaaaagatt ctttgttctc
     2041 attataaaag ctctcgcttt catcttgtga gggctcggct ttcattaaaa cttcattaat
     2101 agccattcct tttaatactt ccttacttag cttatccatt tctgctttat aagcttcaga
     2161 gtcttgaatt ttatttgcaa gagcatcttg ataaagtaat tcttgattaa tcagttcttc
     2221 tactattact tcttcaccta ggcttgccaa ttgttgggca tactcaggag gcatagaagc
     2281 ctgaaaatcc cttaggtcct ctcttgttag agatttattc cctacttttg ctagtacttc
     2341 gttcatttta cacttccttt cttctatcaa cttgttatac ccatataata tcaccataca
     2401 ccttttttag ctagattaag cttctttaac agtaaagccc ttgtttttta gtcttttata
     2461 caaaaacttg actgtttctg ctgttttttg tataatttct ttaagtatcc ttgaaaagac
     2521 agagtaccag gagtttttct cgccagcgag ccggtgatag tgaaagatcg gcaaatatat
     2581 tctgggaatg gaccttggag atactccgct tggaaaagcg ttaccctaaa gaaccaagta
     2641 ggtggtaccg tgcttatgcg ccctattttt gggcgctttt tttataggag gaagaatgaa
     2701 aaaacgaatc ttcagtggca ttcagccctc aggagatctg actctgggca attatcttgg
     2761 agccctgcgc aattggcctc gccttcaaga ggactaccat gcaatctatt ctattgtaga
     2821 cctgcatgct attacagtaa aacaagaccc aaaactactt aaagaaagaa ccctactcac
     2881 cctagctctt tacttggcag ccggcatcga cccggaaaaa tcaattcttt ttatacagtc
     2941 tcatgttccc caacatgccg agttaaattg gttgctggcc tgccacacct atatgggaga
     3001 gctctctaga atgactcagt ttaaagataa gtctagtcgt ttaagccaaa aggacagtat
     3061 cccctcagga ctatttaatt atcccaccct aatggcagca gatatccttc tctatcaagc
     3121 tcagcttgtt cccgttggag aagaccagtc gcaacatctt gaactaacgc gagacctagc
     3181 cattcgattt aataatgcct atgggaaaac ctttgctata ccaaaaggct acttcaatcc
     3241 ctttggcgct aaaatctatg atcttcaaaa ccccaccgct actatgagta aatcatcagt
     3301 caatcaaaat ggcgttcttt ttctaatgga agaagaaaaa agcctgcgaa aaaagattgc
     3361 ccgctctgta acagacaatt tagcagtcat ccgctatagt gatgagcaag ctggggtaaa
     3421 gaacctgcta aatatttatt cccttcttag tggaaaaact cctgataaga cgcttttaca
     3481 ctttgaaggt aagggctata aggcactaaa ggatgatgtt agcgaacttg tccttgatga
     3541 acttcttccc ctacaaaaac gaacattaga atatctgggt gatactgtag agctggagaa
     3601 aatctatagc aagggggcaa aaaaggccaa agaactggct aaacccacgc tagaagacgt
     3661 taaagaaaaa ctaggctttg taatctaaaa tctaaggggg acctatgaat attgcaataa
     3721 aagatggtca attcatatct ctagatgaat ccatggccaa ccgccatggc ctggtggctg
     3781 gggccacagg tacaggaaaa acaatcacca taaaacttat ggccgaagaa tttgccaagg
     3841 caggcgttcc tgtttttatc accgatatta agggggatct ttcgggtttt ttagaagcag
     3901 gcacactaag tccaaaactg gcagagcgcc ttgagaaaat tggggctcct agtcccgaat
     3961 ttgaaagctt tactgttaac ttttgggatg tccttaagga aaatggcata ccccttcgag
     4021 ccaccatcag cgaaatggga cccctgcttc taagtagact cctaaatctt aatgatacac
     4081 aaacaggagt cctctatgca ctttttaaaa ttgcagatga ccaggggctt ctactattag
     4141 attttaagga tctatcggag atgctcaaat tcctatatga aaactctaag gagctttcca
     4201 aagactatgg gaatatgagc aaggcatcta tcggagccat ccagcgcagt cttcttattt
     4261 tagagcaaca aggagccaca gagttcttcc aagaacccgc cttagatgta agagactttc
     4321 tacaaaaaga taggcaggca aggggagtta tcaatatctt agatgcacgt aaaatttacc
     4381 ataatcctga tatctactcc gccttcttgc tgctactttt atccaatctt tatgaagaac
     4441 ttcccgaagt tggcgatcag cccctaccta aacttgtctt cttttttgag gaggctcatc
     4501 ttattttcca aaatgcttcc aaggtactct tggaaaaaat cgagcaaatc gttaagctta
     4561 ttcgcagtaa gggagttggc gtatttttta tcacgcaaaa tcccagggat atcccagaca
     4621 atattcttgc tcaaatgggc aaccgagtcc agcacgccct tagggcctat acccctgcag
     4681 aacaaaggtt ggttaaggcg gtagcagatt cttttagaaa tgatgcagaa atgaatgtcg
     4741 cagaagagct gcttgctctt aaaactggcg aggccttgat ctcaaccctg gatgaaaatg
     4801 gggctcccac ccccgttgtc aaagctttta ttttagctcc tgcctcaaat atggaccccg
     4861 cctctagtca aagcgtggaa aacgccatta aaaactccta tttttataat aaatacgaaa
     4921 aaaccttcga ttcccattca gcctatgaag tgctaaaaga aagggccgag caaatggctg
     4981 aggaagttaa gtcttctgat aaaaaagaaa aagaagaggg actgttagga agccttctta
     5041 agccacgttc tagtagagac acagccatgg agcggatgat aaaaaacacc atgggttcct
     5101 ttggttctca acttggaagg gagattttcc gaggtgtcct aggaggcata aaaaaagaca
     5161 aataataggg ccttattagc ccctcctatt tattgctagc aaggggctaa agtaagaggc
     5221 aaaaggacct aatgaaaact agaagaaagc agtaaaatat gaccaaaaac aaagccttcc
     5281 cctggatttt agtcctttta tggatgggcc tgatctttta tttttctcat caaccagcca
     5341 gccaatccgc tcagctaagc agggggctaa ctgtaacgat tataaatatc atagaaaagc
     5401 ttgcccccaa tatagatttt gactttggcg cctttcatca ttttattcga aaaaacgcgc
     5461 atttttttgc ctacttgatt cttggtctgc tagtagcaaa tgctatgagg gttcgcaaaa
     5521 ccaagagggc tcttgccgca attatcctat gcaccttata tgctcttagt gatgagttcc
     5581 atcaaacatt tatccctgct agggcaggac agtggaccga tgtacttatt gatagtatgg
     5641 gctcagcttt tggtgttttc tcctatcaga ttgctctttt tatgaaaaaa agataatcct
     5701 agatagatta tccttttcat aaaacatagt ggtctttgta aaagaagcca tcctgctctt
     5761 tttttaccta gttggagctg gccacctgta gggatactag cttttttccc cccgctactt
     5821 tttgctgacc tacttcctta aatccgaatt ttttatgaaa aagtaaagat cccggattag
     5881 gaggttcaat gtcaatttct gctgctacac agtccacacc cgtttgtctt gcatgaataa
     5941 agacagactc ataaagtagt tttcctaatt ttttgccgtg cattttggat gataccacta
     6001 ccctatcgat atacaagaac cgctcataat gctcagaaaa ccacaggtaa ttaatgctat
     6061 cgtacttttt tccttctcta aaggttaaaa caaaggcctc cacaagacca tcaatttcta
     6121 ctacattaaa catttccgat tgatctatca ggctatttag tttttctttt gtgagtgggg
     6181 atagaaaatg cactaattct ttgtttatct caagtactct atcgcagtct tttttttcgt
     6241 attttctcat acttatattt ttcatttgct cctccttagt ttcttcttgt cctttacctt
     6301 tacttttttt gtctggattt ttaaagcaat cagggataat ggtaacaata taaggttttt
     6361 tcttaaatac cccaaagcta taatcttttc cctccttgcc ccactaattt tcttcgaaaa
     6421 tgccaattcc taaataattt cataaaaatt ccttcactaa ctagtatggc aataaaaata
     6481 caaagataac ccatactcat agaaaagttt atcggatcct tatggatgat aatgccaaaa
     6541 aaggtcccta caggtgcctc caggcttaat aaaatggagg ccctagtagc cgtagtatac
     6601 ttttgccctg ttgtctgcag catataggct ataaaggtac ttaccacccc aatatatata
     6661 atagccagta ggggttttgc ttctatagga gcaaagcttt taaaaagaag tgctccaata
     6721 agagaaaata aggccgtcac tccaatttgc acaaaattaa gatgaatagg acgttcgtct
     6781 tttacatatt ctgctgagag gaccatatgc aaggccacaa gtaaagcccc gcctaggagc
     6841 aaataatcac cccgggtcaa atttccaaag tttcccgaac taataagata tatcccaaaa
     6901 agactgagga ctgctgctac aaaggtcctg ccctctggga tttttttatt ataaatccag
     6961 tcaaaggctg gtacaaagag cacatagcta ccaactagaa aggctgtctt agaagtgttt
     7021 gttagtcttt gaccttcatg aataagataa aaaaagctaa acattaatag cccaagaatg
     7081 attccttttt ttactgtttg cctggtcaaa tcccctcttc tttttaagga gagaggaaga
     7141 aggcataaaa acccaatcag aaaacgcagg gcctgtatct gaagagaact cacccccata
     7201 ttcaaagcgt attctgtaat agaataacca aaaccccaaa aaagggttac tcctaaaaga
     7261 gctatttctc ctttatactt cacgccttgc ctccaaaata tagtagcctg ccttcttatc
     7321 aaggaccact acattagcaa aaagctcttc caaccttttt tttgcactct tcattccctg
     7381 ctttttttga ataacaatca ttaggcgtcc cccaggataa agatgctgcc ttgcttcttt
     7441 aaagaggaaa tagattgttt cttttcctcg tcgtatagga ggattaaaaa gaatcacatc
     7501 aaacttttta tctatcttgg aaaaaccatc ggagtaaaaa acttttcctt tggccttatt
     7561 taattcaaga ttttttttgg ccagctctag ggccctttga tttacatcac tcatatggca
     7621 cacaattcct aggtgaagat agaccagggt cccaacagga ccatatccac atccaaggtc
     7681 tagtacctcc cccgaaatac ggtctaaact agtctttata agaagatctg tcccaaaatc
     7741 aagacgatct ttactaaaaa cccccctatc tgtaataaaa ggatagctct tcttattaaa
     7801 ataaagagtt atccttttag gatcacttgg gatttttgtt ttttcactaa aataatgctc
     7861 catcctctcc tcctttaaaa atgataagat gaaaaaatat tcctggagct gccagctcac
     7921 taaagtcgat ctcaaccttt cttccccagg tggaaaagat aagtttatcc ccagaaaacc
     7981 ctgttattac cataaaatga ttgacatagg gggtgcttgc cggtggaccc aagaccataa
     8041 tagcaggaag ttctccttta agtaaggctt ttttggcttc tagcagggcc ctttttctga
     8101 taaaacgaaa gaccttggtt tctaggaaaa tcccccttat ccgacaaaaa cgcacagtcc
     8161 tttgcgccca taatcctggg ctaatcatgc cataggtatc ttccttgcta tgtggattaa
     8221 aaaaatgaag gggtttaagc cagtagtaga gggctgtggc taattctaaa tactcttttt
     8281 tatctacatt tttcttttcc aagaaataat aatatatatt tgcagaagca attactccac
     8341 agccacctgc tgcccaaaga ctttttttaa accaggcttg atttccacca tagcttcctt
     8401 gaacaagggg aaagtttttt aactccattt tgcctccttt gtggatatct ttgttttatt
     8461 gtggaaaacc ctcctgttaa aagccttgat tttagcactt atccaaaact tatccacatt
     8521 atccacaaag tatccacagt tttgcctgtg gattaaaggg aaagatttgc ctttgcattt
     8581 agtgaaagag gagcgaaatc ttcttttaag gccttataat aagctgctcc tgcaatcatt
     8641 gctccattat cagtacaaat cgaaagggga ggaataaaga cttgtgccgg aagatccgct
     8701 aagacctccc taagtctttg attggaggcc actcctccag caataatgat ttttctttgg
     8761 cctgtttgtt ctatagcctc tattagtttg tcataaacta tggaaaatac tgtttcttga
     8821 aaagaggcgg ccagatctgc tttctgcttt tctgtaagtt ctagtttatc cgccaggttt
     8881 tttaccgctg atttaatgcc cgagaaggag aagtccaagg aacccttttc cagcatcgtt
     8941 tttggcagcg aaaagctagg ctggccctgg cgcgcaattt cctctacctt gggcccgccg
     9001 ggataggaaa ggcccagtat tcttgctact ttatcaaagg cttcccccag ggcgtcatcc
     9061 ctggttcctc ctaggtattt atagacccca tagtcctggg taagataaaa atgactatga
     9121 cctccactta agataagcga aagaaaggga ggctccaaat catgaaaggc caggtaattt
     9181 gctgctacat ggccatcaag atgatgaatg gcatagaggg gtttatttag agcgtagctt
     9241 agacccttcg cataatttaa gcctacaaga agaggcccta ttaggccagg tccctgagtt
     9301 actgccacac catcaatttc atctagtctt tttcccgatt cttccagggc ccgtgatacc
     9361 actccatcta tacgctctac atgggaacga gaagctatct ctgggacaac tccaccaaag
     9421 ggttgatgga tttcaatttg ggagtggatt acgctagata gaacttttct gccatcttct
     9481 acaagagcgg cggctgtttc atcacagctt gtttcaattc ccagaattag catttttttc
     9541 ctccaatcgc ctcttggcct ctacctctct taaatagatt ggcacaagct cctcttgggc
     9601 ctgtccatct aaataggctt gaatgactcc ctgggctaag gggcctggat ctttaaaaga
     9661 aaaagaagga gcttgctcct taaaggcgtc tagtttttcc ccaacaaaaa caagctcctc
     9721 atccctttct tttaggctct gtaaaaaaac agcgaattct ccgttaaagg gttctttttt
     9781 ataggcacca taggcagccc cataaacatt tcccctacgg gcatccatta ggggcactac
     9841 aatatcagcc ttatgcccat aacttctaga acgaaggcta tttactccat aacaggcaag
     9901 ccctgcccct ctagcaagcc ctaaagcagt agaaaggcca atgcggatgc ctgtgaagct
     9961 tcctggaccc aggcccagga ctagggtatc catgtcctct aggccatagc ctagctcagt
    10021 tagggctcgg tccattaggg gcataagctc actggcatgg ccaaagtttg atgtggtctc
    10081 aaaattatga aagtccccat catatatgca gagggagcag acactgctca ccgtatcaaa
    10141 tgccaaaatc ttctcttctt gcaaatttca ccctccttac ttggtctttt tcctctataa
    10201 tttccaggca ataggcctct ttaggaatta attcaagacc cacctggggc cactcaataa
    10261 caatcctgcc cggtcgatca aagtactctt caaagcctaa agccaaaagc tcctctatat
    10321 ccccaatgcg ataaagatcc atatgaaaaa gggtcccccc cttatagggg tgctcctcaa
    10381 caatggaaaa ggtggggctt tttatgtttt caatccctag agccctacca tatgccttgg
    10441 taaaggtagt ttttcccgct cctaaatctc caattaaaag aagtataggg atttcttttt
    10501 ttgctaaaat atttgcataa ctggtaagtt cttctactga atgtattagc acaatctctc
    10561 cttaataaaa aaccttataa ataataacta gtattgctat atggacagga taaaaaaagt
    10621 aaaaaagata ctttggaact ctttttttgg gaagtctttc actgatctga attaggggta
    10681 aggcaagaat actccgccac tgaaatataa acccatcaag accagcaaac ccctgaataa
    10741 taacagaaaa agaagttcca tatattagtc cactggaaat actcccatag atacttatta
    10801 atacatatcc tagaatctgc tcaaagggat cccggatgag ataaaacaga aggctcagca
    10861 taagaccata acttccatag cccagagaaa agggaggaaa aaagtactgt ataaggtctg
    10921 gtagtagtag taatcccata gcaagcaaat aaaaccattt actctttttt cttttctcat
    10981 aaagattcag aaggataaaa ctcaaagcaa actgaaatag ctgatttaat ttataaagag
    11041 taaaaactgc cccaggattc aaaaagctgt agggcaactg gctaataaaa gcaaaaacca
    11101 ccagcctttt aaaataggcc ctccgattgg aactatagcg aaacccctgg gccaaagccc
    11161 aggcaaagat aggaa
//
LOCUS       DASRNA010000072         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_919304,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000072 DASRNA010000000
VERSION     DASRNA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919304"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..851
                     /locus_tag="VFD08_02070"
     CDS             207..851
                     /locus_tag="VFD08_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09835.1"
                     /translation="MKKSWFLILLILLLVPGAISVCKNKEEAVEGFNLSKKSEKKAYS
                     QDFHIQPKTEKPKKTRQETALKKPKKTARKIEEIFQEESQEPLPEKEESKLEKAQGKE
                     AGEKEEERDKPVDIPKEENALINRNQSEVIEEKTENKREEGFVFEPYYSAPIGSQGLF
                     SDSDSATDEGWRISEMDNPSEYLTFVPKGWEVYPVWYYTSPDEFVVFYTLNFYD"
     gene            903..>4205
                     /locus_tag="VFD08_02075"
     CDS             903..>4205
                     /locus_tag="VFD08_02075"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HZK09836.1"
                     /translation="MKRQNIALLLVLLLLFTNMIPLSEAYKEDLGEKTTEELDKKQSA
                     LKEDKKAPLEITRILLDEDAVVEKELVEIDKDWIDLAEYIDKDQAFKSLENELKKEVE
                     YVGRRSQNGELLEEVEEIELETLEKKEGQFILILEYKVKEETLLEEEKELRHLLVKEE
                     EKDYQVILPKEAGEQNASSPALLASTNSKKVTVTPIDPRGSWAFFHVWDTKKPPITGI
                     TSTFAYFKLADGSIGFCMDPGLGAPQGDLPVDVFANSEFRHFAEKVATLAISSGGIPG
                     YSFEENYVFAQMYIWKNIPLAGESKNRYVLDFRYQHSPFEQHKETMAKYLAWKNEIDR
                     KMNHLGKISFDGNSLELELGKEYRLEDKNKNLHAFNIKTNNADLYVRKEANTLILKAQ
                     KPLNTRLEFWQARNSYPLPEVTYLLKGHDASYQDLGVFRDPFVATLRVSSKQTVARAK
                     IIKRDKDKNSIRGAVFQVSSSRDFKQILEEITSNNDGIALSKEYSINQYPKLYIREKS
                     VPEPYIKSEEIKEIILDSAKTVETTYINDTKKVRLYKKDFFTGKAISGAVIELTDPSG
                     NKKEYRTDQEGKVEIIGIKSGAYSFKEKASPKGYTLNKKSFTFSVTSKGQISGTTELI
                     NKPTRLRLLKKDKNTGQPLAGAGFEVYAMEEKEGRRQIQATYDKDQGRYLLAEKGESI
                     FFSNDRGEVLIDHLPQGDYLVVEVQASPGYKLGSSGRPVEKTLRIDKNGQLSPKEVEF
                     FNPPTEVVLQKKDRLTGKPVAAAKIEITRKGQLIKSLVSDEKGQVKVLGLTSGTYDYR
                     EIEAPSGYVLEDKTYSFTIDGAGNTRGTTEFTNEPTRLRLLKKDKNTGQPLAGAGFEV
                     YAMEEKEGRRQIQATYDKDQGRYLLAEKGESIFFSNDRGEVLIDHLPQGDYLVEETKA
                     LAGYRLPQDISKRSKIIIIDNDSKQGAPASVFLNEPTQVILTKKDMLTGIALSGAEIE
                     VSINDKKSKFTSDEKGQIELIGLKPGSYSFKETKQPLGYILSKEEFIFFIDQKGHARG
                     VSEFINEPTRLIVKKTDEKNKAMKGVVFELRNSEGKIIKANYDKKSGRYLVAEKGENK
                     NFISNKQGTISID"
BASE COUNT         1571 a          701 c          936 g          997 t
ORIGIN      
        1 agaagtagga gctaaaaaag ctctaagaga tgcgctagta gatctttatg tgaagttata
       61 ttagctatta aaaaaagagg gaaatcaccc ctcttttttt tacaccaaat tggaatactc
      121 atgaaaaata aagataaaac catgaaataa acctatactg gatttgaaat aaaacaagaa
      181 aaatatagga aagacaggtg taaaagatga aaaagtcctg gtttcttatt ctactgattc
      241 ttcttttagt tccaggggct ataagtgttt gtaaaaataa agaagaggcc gtagagggat
      301 ttaaccttag taaaaaaagt gaaaaaaaag cctatagtca agacttccat atccagccaa
      361 agactgaaaa accaaagaag accagacaag aaacagctct aaaaaaacct aaaaaaacag
      421 caagaaagat agaggaaatt ttccaggaag agtcccaaga acctttgcct gaaaaagaag
      481 aaagcaagct ggaaaaagcg caggggaaag aagccggtga aaaagaagaa gaaagggaca
      541 agccggtaga tattccaaaa gaagaaaacg cgcttataaa taggaatcaa agcgaggtaa
      601 tagaagaaaa aacagaaaac aaaagagagg aggggtttgt ctttgaacca tactacagtg
      661 ctcctattgg tagccagggt cttttttccg atagcgactc tgctacagat gagggctggc
      721 gtattagtga gatggataat ccttcagaat acttaacctt tgtccctaag ggatgggagg
      781 tgtatccggt ttggtattat acttcgcctg atgagttcgt ggttttttac acattaaact
      841 tttatgactg aagatttata agtaaaggca ggtctgcatt attttaaagg gaggaaatat
      901 atatgaagcg ccaaaacata gccttattac tagttctttt attacttttt accaatatga
      961 ttcctttatc tgaagcttac aaggaggatc taggagaaaa aacaacagaa gagcttgata
     1021 agaagcaaag tgctcttaaa gaggataaga aagcccccct tgaaatcacc cgaattcttc
     1081 ttgatgaaga cgcagtggtt gaaaaagaac tagtagaaat agacaaagac tggattgatc
     1141 tggccgagta tattgataaa gaccaggcct ttaagagcct agaaaatgag cttaaaaaag
     1201 aggtcgaata tgtaggaagg cgcagtcaaa atggagaact actagaagaa gtagaagaga
     1261 ttgaattaga gactttagag aaaaaagagg gtcaatttat tcttatccta gaatataaag
     1321 taaaggaaga aactttatta gaagaggaaa aagagctaag acacctactt gtaaaagaag
     1381 aagaaaaaga ctatcaagtt atacttccta aagaagcagg ggagcaaaac gcatcttctc
     1441 ccgctctttt agctagcaca aatagcaaaa aggttacggt aactcccatt gaccctagag
     1501 gtagttgggc cttttttcat gtttgggata caaaaaaacc tcctataacg ggtataactt
     1561 ctacttttgc gtattttaaa ctagcagatg gtagtattgg cttttgcatg gatccaggtc
     1621 ttggagcacc gcaaggagat ttacctgtag atgtttttgc taatagcgaa tttaggcatt
     1681 ttgcagaaaa agtagctaca ctggcaatta gttcaggtgg gattcctggc tattcctttg
     1741 aagaaaatta tgtatttgct cagatgtata tttggaaaaa cataccctta gccggggaat
     1801 cgaaaaaccg gtatgtcttg gattttagat atcaacatag tccctttgaa caacataaag
     1861 agactatggc aaaatattta gcgtggaaaa atgagattga tcgtaaaatg aaccatcttg
     1921 gtaaaatatc ttttgatgga aatagccttg agcttgagct aggaaaagaa tatagactag
     1981 aagacaaaaa caagaaccta catgccttca atataaaaac aaacaatgca gacctatatg
     2041 ttcgaaaaga ggcaaatacc cttatattaa aggcacaaaa gcctttgaat accagactag
     2101 agttttggca agccagaaac tcctatccct tacctgaggt gacttatctc ctaaagggac
     2161 atgatgccag ttaccaggat ttgggagttt ttcgtgatcc ttttgtggcc actcttaggg
     2221 tgagctccaa gcaaacagtg gcaagagcaa aaataataaa aagggataag gataaaaaca
     2281 gcattagggg agctgttttt caagtctcta gtagcagaga ctttaagcag atcctagaag
     2341 aaatcactag taataatgat ggcatagctc ttagcaagga atattcaata aatcaatacc
     2401 caaagctcta tattagggaa aaaagcgttc ctgaacccta tattaaaagt gaagaaataa
     2461 aagaaattat attggattca gcaaagacag tcgaaacaac ctatataaat gacaccaaga
     2521 aggtaagatt atataagaag gatttcttta caggaaaagc tatatcaggt gcagtaattg
     2581 agctcaccga cccctcagga aataagaaag aatatagaac tgaccaggag ggaaaagtcg
     2641 aaattattgg tataaagagt ggggcctaca gcttcaaaga aaaagcaagt ccaaagggtt
     2701 acacccttaa caagaagagt tttacttttt cggtaactag caagggacaa atcagcggca
     2761 ccactgaact tatcaataaa cccacccgtc ttcgcctgct aaaaaaggat aaaaacacag
     2821 gccagcccct ggcaggggcg ggctttgaag tctacgctat ggaagaaaaa gagggaagaa
     2881 ggcagattca ggccacctat gataaggacc agggtcgcta cctgctagca gaaaagggag
     2941 agagtatctt ctttagtaat gatagggggg aagttctaat tgatcatctg ccccaaggag
     3001 actatcttgt ggtagaggtc caggccagcc ccggctataa gcttggcagc agtggaaggc
     3061 cagtagaaaa aacccttagg attgataaaa atggccagct cagccccaag gaagtagagt
     3121 ttttcaaccc tcccacagaa gtagtactcc aaaaaaaaga taggctgaca ggtaagccag
     3181 tagctgcagc aaaaattgag atcactagaa aaggtcaact aataaaaagc ctagttagcg
     3241 atgaaaaggg ccaggtaaag gtgctgggtc ttaccagcgg cacctatgac tatagggaaa
     3301 ttgaggcccc cagtggctac gtactagagg ataagactta tagtttcacc atcgatgggg
     3361 caggaaatac aagggggacc acggagttta ccaatgagcc cacccgactt cgtctgctaa
     3421 aaaaggataa aaatacaggc cagcccctgg caggggcggg ctttgaagtc tacgctatgg
     3481 aagaaaaaga gggaagaagg cagattcagg ccacctatga caaggaccag ggtcgctacc
     3541 tgctagcaga aaagggagag agtatcttct ttagtaatga taggggggaa gttctaattg
     3601 atcatttgcc tcaaggagac tatcttgtgg aggaaacaaa ggctctagct ggatatagat
     3661 tgccccaaga tatttctaaa agaagcaaaa taattattat agacaatgac tctaaacaag
     3721 gggctcctgc cagtgttttt ttaaatgaac ccacgcaagt tattttgaca aagaaggata
     3781 tgctaacagg gatagctcta tctggtgctg agattgaagt atcaataaat gataaaaaaa
     3841 gcaaatttac aagtgatgaa aaaggacaga ttgagctaat tgggttaaaa cctggcagct
     3901 actcatttaa agaaacaaaa caacccctag gctatatcct ttcaaaagaa gagtttattt
     3961 tctttattga tcaaaagggc catgcaaggg gagtgagtga gtttatcaat gaaccaactc
     4021 gcttaatagt gaaaaaaacc gatgaaaaaa acaaggccat gaagggggta gtttttgagt
     4081 taagaaactc agaaggaaaa ataattaaag caaattacga taagaaaagc gggcgctatt
     4141 tggttgcaga aaaaggagaa aataagaatt ttattagcaa taaacaaggg actatatcta
     4201 tagac
//
LOCUS       DASRNA010000073         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_620368,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000073 DASRNA010000000
VERSION     DASRNA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620368"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VFD08_02080"
     CDS             complement(<1..845)
                     /locus_tag="VFD08_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZK09837.1"
                     /translation="MTTDQALILILLLVILANFIWGKFRFDFVALAGLIIATFLGLVP
                     ANQAFIGFSHPAVITVAAILVLSEGLIRSGFVDAMVKTLEKKAKTPLKQFLFLVYGVA
                     FLSAFMNNVGAMALFMPVAIKIARKNGDSPSRYLMPMAFASLLGGMITLVGSPPNIII
                     STFREEISGSGFSMFSFAPIGLALTFFVIPFIIILASRLIPIRRENVSQEDLFEIKDY
                     TTMVELTEGCSLIGRNLLEMGEEYFSEVNVIGYVRRGRKRARVSPYLNFREKDRIILE
                     ASAYS"
     gene            complement(1039..1890)
                     /locus_tag="VFD08_02085"
     CDS             complement(1039..1890)
                     /locus_tag="VFD08_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK09838.1"
                     /translation="MATRAEYYKKTSSGVQCLLCPHHCRLTDGQLGLCKARICRGKDL
                     YSLSDGKITAMAYDPLEKKPLKYFMKGPIFSIGSMGCNFHCDFCQNFRLLDPQVSSIK
                     ISDKKLLQAAADRGSVGIAYTYNEPTINFEMVKRLSKKIHKQKQVNVMISNGYIEKEP
                     LKELLPLIDAWNIDYKMPADLYGEVCGGKEQVVLETIREVSLHSHVEVSTLLIPGRNT
                     DEKVFRGMMEKLSSINPEIPLHLSRYSPCYKCTIPPTPASTMLACRKIAREYMQRVEL
                     GNMPLYY"
     gene            complement(1880..>2893)
                     /gene="amrA"
                     /locus_tag="VFD08_02090"
     CDS             complement(1880..>2893)
                     /gene="amrA"
                     /locus_tag="VFD08_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZK09839.1"
                     /translation="PLLFLDKRGARKLGSMELDHGATVPLYFLEKHWKVKPKLIHINY
                     GLLSSKELAYFGGLLRRSIRLSKKKVVVLASGDLSHRLKKEGSYDYNPQGPLFDAEVG
                     RIIKEGKLAQFLEMDKDMVEQAGECGLRSLQILAGLLEGQPISSEVLSYEGPWGVGYM
                     SAYFKMEEEKDKLLLLAKKALESHIIDKKPLEPGEDYRELTKEKRACFVSLHKFGKLR
                     GCIGTIEPTKDNLALEIISNAQSAASEDSRFSPVKRDEIEDLVIGVDELGPIEACSFE
                     DLDPKNYGVIVKKGYRQGLLLPDLAGVDTPQEQVAIAREKAMIGPEEKVTYFRFKVTR
                     HGN"
BASE COUNT          820 a          656 c          548 g          869 t
ORIGIN      
        1 atgctatagg ctgaggcttc aagaataata cgatcctttt cccgaaagtt taggtaggga
       61 gaaaccctag cacgttttct tccccttctt acataaccaa ttacgttaac ttcactaaaa
      121 tactcttcac ccatttctag aagatttctc ccaataagag agcatccttc tgtaagctcc
      181 accatagttg tgtagtcttt gatttcaaaa agatcttcct ggcttacatt ttcgcgccga
      241 atggggatta gtctggaggc caagataata ataaagggaa tcacaaaaaa agtaagggcc
      301 aggcctatgg gcgcaaaact aaacatacta aagccactgc cgctgatctc ttctctaaag
      361 gtggagataa taatattggg aggactgccc acaagggtaa tcattccccc tagtaaagag
      421 gcaaaggcca tgggcatgag atagcgggag ggagaatcac cattcttacg ggctatcttt
      481 attgccacgg gcataaaaag tgccatggca cctacattgt tcataaaggc tgataaaaaa
      541 gcaaccccat agaccaaaaa taaaaactgt ttaagagggg tttttgcttt tttttctagg
      601 gtctttacca tagcatcaac aaaaccagaa cgtatcaggc cctcacttaa tactaggatg
      661 gcagctacgg taataaccgc aggatgggaa aaaccaataa aggcttgatt ggctggaaca
      721 agtccaagaa atgtcgctat aattaaacct gccagggcaa caaagtcaaa gcggaactta
      781 ccccatataa aattagctag aattactagc aaaagaatta gaattagagc ctggtctgta
      841 gtcaagtttt tctcctttgt aaagaaattt tatgtctaca ttgtaccaat taaatggtct
      901 tctgtcgatt gcttctagct aaaggattgg cttcttattt cattattttt cctcttagtc
      961 taaaggatgg aaaaaaataa gcgccatttt tctttggcgc ttatacacct tgctttgctc
     1021 ccatttaagc actgctttct agtaatataa aggcatattg ccaagttcta ccctctgcat
     1081 atactccctt gcaatcttcc tgcaagcaag catggtagaa gctggcgttg gtgggatggt
     1141 gcatttatag cagggactgt aacgacttaa gtgaagaggg atttctggat taatacttga
     1201 gagtttttcc atcatccctc gaaaaacttt ctcatcggta tttcttccag gaattagtag
     1261 ggtgctaacc tctacatggc tatgtaaaga gacctcccta atggtttcta gaacaacctg
     1321 ctcttttccc ccacagactt ccccgtataa atctgctggc atcttgtaat caatattcca
     1381 tgcatctatt aaaggaagaa gttcttttag gggctccttt tctatatagc cattgcttat
     1441 cataacattg acctgctttt gcttatggat tttttttgaa agacgcttaa ccatttcaaa
     1501 gttaatagta ggctcattat aggtataggc aatacccact gagcccctat cagccgctgc
     1561 ttgaaggagt tttttgtcgc tgatttttat tgaagatact tgaggatcta gtaggcgaaa
     1621 attttggcaa aagtcacagt gaaaattgca gcccatactt cctatagaaa aaattggccc
     1681 cttcataaaa tactttaagg gttttttctc taggggatca taagccatag cagtgatttt
     1741 tccatcacta aggctataga gatcttttcc cctacaaatt ctggccttac acaggcccaa
     1801 ctgaccatct gttaatcggc agtggtgggg acataaaaga cactggaccc cactacttgt
     1861 ttttttataa tattctgctc tagttgccat ggcgtgtcac tttaaagcgg aaatatgtta
     1921 ctttttcctc aggaccaatc atggcctttt ctcttgcaat agctacctgc tcctggggcg
     1981 tatcaacccc tgctagatca ggaagaagca gtccctgcct atagcctttt ttaactatta
     2041 ccccatagtt tttcggatct aggtcctcaa aagagcaggc ctcaatggga cccaactcat
     2101 caaccccaat gacaagatcc tcaatttcat cccttttcac gggcgagaag cggctatctt
     2161 cgctggcagc actctgggca tttgaaatta tctcaagagc cagattgtcc ttggtgggtt
     2221 caattgttcc aatgcatcct cgcaatttac caaatttatg caaacttaca aagcaagccc
     2281 ttttctcttt tgtgagttcc ctatagtctt ctcctggctc aaggggtttt ttgtcgataa
     2341 tatgactttc tagggctttt ttcgccaaaa gaaggagctt gtccttttct tcttccatct
     2401 taaagtaggc actcatatag cccactcccc agggtccttc atagcttaag acctcactag
     2461 aaataggctg accctcaaga aggccggcta aaatttgaag gcttcttagg ccacattccc
     2521 ctgcttgctc gaccatatcc ttgtccatct ctaggaactg ggctagcttg ccctccttta
     2581 tgattcggcc cacttcagca tcaaataaag gcccctgggg attataatcg taagaacctt
     2641 ccttttttaa acgatgggac aaatcacccg aagccaaaac taccactttt ttcttactaa
     2701 ggcgaatact tcttcttaag agcccaccaa aataagctag ctctttagaa gacagtaggc
     2761 cataattaat atgaataagt tttggtttaa ccttccagtg tttttccaga aaataaagcg
     2821 ggactgtggc accatgatct agctccatgc ttcctagttt tcttgctccc cttttatcaa
     2881 gaaaaagtag ggg
//
LOCUS       DASRNA010000074         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_542119,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000074 DASRNA010000000
VERSION     DASRNA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542119"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..631)
                     /locus_tag="VFD08_02095"
     CDS             complement(89..631)
                     /locus_tag="VFD08_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HZK09840.1"
                     /translation="MVNLEQVEKLRQYGDITYDQAKRALEDAGGDILEAIIVLEKEGL
                     INKPQNGGYHSSKNEGASKDESSQSEKTSSETEDEGRSFSELVEKFFAGFGKLVKRGN
                     QNHFQIAKDGEVVSHISLTILVLLLVFAFWLIVPLAIVGLFFGYSYSFKGPDLGKEGV
                     NKVMDSAAEAVDKIKKESGK"
     gene            complement(874..1482)
                     /locus_tag="VFD08_02100"
     CDS             complement(874..1482)
                     /locus_tag="VFD08_02100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZK09841.1"
                     /translation="MKLYISRHAQTEWNKVMRLQGWKDSPLSEKGLREAHLLKDYLAD
                     IKFDRIYTSDKKRAIDTAKIIKGPRHIEIVELKELRELGMGDWEGKLISEIMEEEGDL
                     FDKYLNFPLSYPAPLGEDIPSLFKRVEFALEKIEGHGGEKILLVSHGNTIRALLVILK
                     KLGMDYYKKAPIYPGTALSIFKKEANAWISMVEGDQSHCLDE"
     gene            complement(1531..2580)
                     /locus_tag="VFD08_02105"
     CDS             complement(1531..2580)
                     /locus_tag="VFD08_02105"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZK09842.1"
                     /translation="MEHGGDLLTYKDKYGGKLIDFSSNINPLGVPRGLEKAIMDAFKE
                     VEVYPDIQYRKLKEALANYLDCEKANILVGNGAVEIIDNFIMLAERVVLFIPSFSEYE
                     LRAGVHKKEVLVLEYGDDFTIEVDILKKNLKENDLLILGNPNNPSGLRIAEETLLEIY
                     ACVQSVKGHLLLDEAFYEFCPKDYDSIELFKDTAYKNVSVIRAATKFFALAGIRLGYA
                     CGSQGTVEKISSIELPWRINSMANTAGLTIFKDTDYIKKSKDYIRRQRDYLFSELEKI
                     KAVKPYTSHTNFILIKLLDLEEDYLFEELLKKGILVRKCSSFKGLGRDHIRVAVKDKK
                     SNDLLIKALKDITKV"
     gene            complement(2591..>2817)
                     /locus_tag="VFD08_02110"
     CDS             complement(2591..>2817)
                     /locus_tag="VFD08_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HZK09843.1"
                     /translation="ESPNSGYPESAVSGLLGIQLGGSHLYKGVLVEKPTIGDKQVDTK
                     TIHVKHSIQIMYRSQISFIIIYIVIWLLIK"
BASE COUNT          767 a          615 c          417 g         1018 t
ORIGIN      
        1 tatttaagtt ctagctctat aaagcgaact attttttctt catcttcaat aatcagaatc
       61 ttctccatta agaatttcct ttactttgct atttgccact ttcttttttt atcttatcta
      121 cggcttcagc tgctgaatcc attaccttat tcacgccctc tttgcccagg tctggaccct
      181 taaatgaata gctatatcca aagaacaagc caacaattgc taagggtaca ataagccaaa
      241 aggcaaatac tagtagcaag accaagatgg taagagagat gtggctgaca acttccccgt
      301 cttttgctat ttgaaaatga ttttgatttc ctctttttac taattttcca aagccagcaa
      361 agaatttttc tacaagttca gaaaaactcc tgccctcatc ctctgtttca ctgctagttt
      421 tttctgattg agaactctca tccttgctag ccccttcatt ttttgaacta tgatagcctc
      481 cattttgagg tttattaata agtccttctt tttctagaac tataattgcc tctagtatat
      541 ccccacccgc atcttcaagg gctctttttg cctgatcata agtaatatca ccatactgac
      601 gtagtttttc tacttgttcc aaattaacca tattaacttc ctccccttat ttagtatggc
      661 ctaagcataa caattgtatc tttgaggatt gttcatctaa gattaaaatc tcattagaac
      721 tttttgtaat aatattatat catgctttta aaaaagtagg tcccagaaat ccctagctaa
      781 gctggctaaa agtctggcga ggagttcttt tagaggaaat atcatttttc ttctgcctat
      841 agggctaaaa acaaagatac taaagttgct actctattcg tccaaacaat gggactggtc
      901 tccctcaacc atgctaatcc aagcatttgc ctctttttta aaaatgctta aggctgtccc
      961 tggataaata ggggcttttt tatagtagtc catgccaagc tttttaagta tgactagcag
     1021 ggccctgatt gtatttccat gggataccag taaaatcttt tctccaccat ggccctcaat
     1081 cttctctagg gcaaattcca ctcttttaaa aagactggga atatcctcac ctaaaggggc
     1141 gggataagaa agtgggaagt ttagatattt atcaaataag tctccttctt cttccatgat
     1201 ctctgatata agtttaccct cccagtctcc cataccaagt tctcttaatt cttttaactc
     1261 tacgatctct atatgcctag ggccttttat tattttggcc gtgtctatcg ctcttttttt
     1321 atcgcttgtg taaattctat caaatttaat atcagccaga taatctttaa gtagatgggc
     1381 ttctcttagc cctttttcac taaggggaga atctttccag ccctgaagcc tcataacttt
     1441 attccactct gtctgtgcat gtctgcttat atatagtttc aagtctaccc ctcgtttttc
     1501 ttataaagtg tcttaggcct ggttgcatta ctagactttt gttatatctt ttagggcctt
     1561 gattaaaaga tcattgcttt ttttatcctt aacagcaacc cttatatggt ccctgcccaa
     1621 tcctttaaaa gaagaacatt ttctcaccag tattcctttt tttaataact cttcaaatag
     1681 gtagtcctcc tctaagtcta gtaatttgat caggataaag tttgtatggc ttgtgtaggg
     1741 ctttactgct tttatcttct ctaattcaga aaataagtag tctctctgcc ttcttatata
     1801 atccttactc tttttgatat aatcagtatc tttaaatatt gttagacctg cggtatttgc
     1861 catggaattt attctccaag gcagttcaat gctgcttatt ttttcaacag taccttggct
     1921 tccacaggca tagcccagtc taattccagc cagggcaaag aattttgtag cagctcttat
     1981 tacgcttaca tttttatagg cagtatcttt aaataattca atagaatcat aatcctttgg
     2041 gcaaaattca taaaaagcct catcgagtaa taagtgtccc tttacacttt gaacacaggc
     2101 atatatttct aataatgtct cttctgctat tctcagacca ctaggattat tggggtttcc
     2161 cagtatcaag aggtcatttt cttttaggtt tttctttaga atatctactt ctatagtaaa
     2221 atcatccccg tattcaagca ctaagacttc ttttttatgg acccctgccc ttagctcata
     2281 ttcggaaaaa gatggaataa aaagtaccac tctctcagct agcataataa agttatcaat
     2341 aatttctact gcaccatttc caacaaggat attggctttt tcacagtcta ggtaatttgc
     2401 tagcgcttct tttagttttc tgtactggat atcaggatag acttccacct ctttaaaggc
     2461 gtccattatt gccttttcta atcccctggg gactcccagt ggatttatat tgctgctaaa
     2521 gtcgattagc tttcctccat acttatcttt gtatgttaaa agatctccac catgctccat
     2581 taaaacctcc ctatttaatt aacagccaaa ttactatata gattataata aagctaatct
     2641 ggcttctata cattatctgg atcgaatgct ttacatgaat tgttttagtg tccacctgct
     2701 tgtcacctat agtgggtttt tcaactaaaa ctcccttata aaggtgactc ccccctagct
     2761 ggattcccaa taaacccgaa acagcacttt caggatagcc gctatttggg ctttcat
//
LOCUS       DASRNA010000075         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_25834,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000075 DASRNA010000000
VERSION     DASRNA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25834"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFD08_02115"
     CDS             <1..473
                     /locus_tag="VFD08_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK09844.1"
                     /translation="FSGTLRQNMLWGDSKASDEKIKWALEKAQAWEFVKAYDKGLDHL
                     VEQGGTNFSGGQRQRLSIARSLIKNPKIMILDDSTSAVDVTTDAKIRQAFSQELAEMT
                     TLIIAQRISSVEDADRILVMEEGRIHAIGKHDELLETSTVYREIYDSQQKGLPS"
     gene            470..2287
                     /locus_tag="VFD08_02120"
     CDS             470..2287
                     /locus_tag="VFD08_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK09845.1"
                     /translation="MKKQKSLKPKNLGATLMRMFSYFRYNLGLFVFGMISVLLATLAT
                     VGFNGMLSPIIDSLVPGPKKSFYTYLVYMVVLILINVVFQYFGTLSMARLAQRTVHRI
                     RQDMFHHLQKLPISYYDSQIQGELMSTFTNDVDMLNQSLEQSLSQIIIAIVSFVGSII
                     MMLILSPILTLVVAFMLGVMAFAVSFIGKRSATFFRRRQSALANMNGYVEEMISGHKV
                     VKVFNYEDRNQEYFDEKNEELRLSSTAASTYGVMMMPVMGNLSHVMYALVAMFGGILA
                     IAGSLTIGNIAAFLQYTRTITRPITQVSNQLNTLLAALAGAERIFAVLDEPEEEDQGE
                     VRLIEDCDTFNGYCWLVPEGDELKRIPVHGDIRFYDVNFGYKKDQQILHDISLYAKPG
                     QKIALVGSTGAGKTTITNLINRFYEINGGKITFDGIDLRSIHAHDLRRTLGMVLQDVH
                     LFESSVRENIRYGRLDASDEEVVKAAKMANAHHFIQLLPKGYDTVLSIDGANLSQGQR
                     QLLSIARAAISDPIVLILDEATSSIDTRTEKMIESAMESLMENRTTFIIAHRLSTVRR
                     ADAIMVLEEGQIIERGDHLDLMNQKGRYYELNMGLAKLD"
BASE COUNT          685 a          506 c          501 g          668 t
ORIGIN      
        1 ttttttcggg taccctccgc caaaatatgc tttggggtga tagcaaggcc tccgatgaaa
       61 aaataaaatg ggccctggaa aaggcccagg cctgggagtt tgttaaagcc tatgataagg
      121 gcctagacca cctggttgaa caagggggca caaacttctc tggaggccag cgccaacgcc
      181 taagcattgc taggagcctc attaaaaatc cgaaaattat gattttagat gactccacct
      241 cagcagtaga tgtcacaaca gatgcaaaaa tcagacaggc cttctcccag gagctggcag
      301 aaatgacaac ccttattatc gctcaaagaa tttcctctgt tgaagatgcc gaccgtattc
      361 ttgttatgga agaaggtcgt attcacgcca ttggaaaaca cgatgaactt cttgaaactt
      421 ctacagttta tcgtgagatc tatgattccc agcagaaagg tttaccatca tgaaaaaaca
      481 aaaatcgcta aaacctaaaa acttgggagc taccttaatg agaatgttct cctattttcg
      541 atataatctt ggcctttttg tttttggaat gatcagtgtg cttcttgcca ccctggccac
      601 tgttggcttt aatggtatgc ttagccccat aatagatagc ctagttccag gtccaaaaaa
      661 gagtttctat acctatcttg tatatatggt agtgcttatt ttaataaatg tggtttttca
      721 gtattttggg accctttcca tggcgcgact tgcacaaaga actgtccacc gaatccgcca
      781 ggatatgttc catcacctac aaaaacttcc tatctcctac tatgatagtc aaattcaagg
      841 agagttgatg agtaccttta ccaatgatgt ggatatgctc aaccagtctt tggaacagag
      901 tctttcacaa attattattg ccattgtatc ctttgtggga agtattatta tgatgcttat
      961 tttaagtccc atccttacac tggttgtggc ctttatgctt ggagttatgg cctttgctgt
     1021 ttcttttatc ggtaaaagaa gcgctacctt ttttagaaga agacagagcg ctcttgcaaa
     1081 tatgaatggc tatgtcgaag aaatgatctc aggacataag gtggtaaagg tttttaatta
     1141 tgaagataga aatcaggagt acttcgatga gaaaaatgaa gaactccgcc ttagctcaac
     1201 tgcagcatct acctatggag taatgatgat gcccgttatg ggcaatctct cccatgttat
     1261 gtatgctcta gtagctatgt ttggggggat tcttgctatt gctggtagct tgaccattgg
     1321 taatattgca gcctttttgc aatacaccag aaccattacc cggcccatca cccaggtttc
     1381 caatcagtta aatacccttt tggctgccct ggctggagcc gaacgtatct ttgctgtcct
     1441 tgatgaaccc gaagaagaag atcagggaga agtgcgcctg atagaggact gtgacacttt
     1501 taatggctac tgctggcttg tccccgaggg agatgagctg aaaagaatcc ctgtccatgg
     1561 cgatatacgc ttttatgatg ttaactttgg ctataaaaaa gaccagcaaa tcctccacga
     1621 tatttctctc tacgcaaaac caggacaaaa aattgccctg gtgggcagta ctggggcagg
     1681 aaaaacaacc atcaccaatc ttattaatcg tttttatgag atcaatgggg gcaaaattac
     1741 ctttgatgga attgacctta gatccattca tgctcatgat cttcgcagaa ctctgggaat
     1801 ggtacttcaa gatgtgcatc tttttgaaag ctcagttagg gaaaatatcc gctatggaag
     1861 gcttgatgcc agcgatgaag aggttgtaaa ggcggcaaaa atggccaatg ctcaccactt
     1921 tattcaactg ctgcctaagg gctatgatac tgttttaagc atcgatggag ccaacctatc
     1981 ccaaggtcag aggcagcttc tttctattgc aagagcggcc atctcagacc ccattgttct
     2041 aatccttgat gaggcaacct cctccattga tactagaacc gaaaagatga ttgaaagtgc
     2101 tatggaatct cttatggaaa accgtaccac ctttattatt gcccatagac tttccacagt
     2161 aagacgggct gatgcaatta tggttctaga agaggggcag attatagagc gaggagacca
     2221 tctagaccta atgaatcaaa aagggcggta ctatgaactc aatatgggcc tagccaagct
     2281 ggattaaaag aaagggccca ggccctttct tttaatctcc ccttaataaa caaatcccac
     2341 cccaacatat tacatatact
//
LOCUS       DASRNA010000076         7469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_921247,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000076 DASRNA010000000
VERSION     DASRNA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921247"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFD08_02125"
     CDS             <1..891
                     /locus_tag="VFD08_02125"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZK09846.1"
                     /translation="ENTPAIIHGGPFANIAHGCNSVLATELGLLTSDITVTEAGFGAD
                     LGAEKFFNIKCRSAKLNPDAVVLVATIRALKMHGGKAKNELANEDLGALKKGVVNLEK
                     HMENLALFGVPFMVAINTFPTDTEAELSWLFDYVKDLGVPIARSEVFAKGGQGGMEMA
                     KTLLELMEKEESHFTPLYDNEMPIKEKIETIAKKIYGASDVHYDKSALKQLKDIKDIG
                     LDHLPVCMAKTQYSFSDDPTLLGRPEDFDLTIRELRISAGAGFIVALTGAVMTMPGLP
                     KRPAALNMDVLDDGEVVGLF"
     gene            891..1814
                     /locus_tag="VFD08_02130"
     CDS             891..1814
                     /locus_tag="VFD08_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZK09847.1"
                     /translation="MKVLYCTPIEPEGEKRIQDLGYKVVAKKEKDLRQEDLENVDVFV
                     GMNPFSKIDFTKGSLKFIQLQTQGFDHLPKNDLEGVLVANNHGGYAIPIGEWIVTKIL
                     ESFKHTRNIYNLEKKRQWKKDFGIEELTGKKVLFVGTGTIASEAAKRLLPFGVEIFGT
                     NRSGKSAPFIEKILKLDELDEILPQVDVLVPVLPATKDTEDLIDASMLKALKAGSVIV
                     NISRGQIIDEKALLEVIDKFLAVHLDVTKQEPLPVDHPLWEKENVYISAHTSWVSQYV
                     DKRRDDLIYENLRRFIQAEELLNLIDLRRGY"
     gene            1814..2839
                     /gene="rlmN"
                     /locus_tag="VFD08_02135"
     CDS             1814..2839
                     /gene="rlmN"
                     /locus_tag="VFD08_02135"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZK09848.1"
                     /translation="MNLLGMSYEEVMKIDFNQPNYRNKQIFEGLHKQGIDYEKMSSLP
                     LKLRKQLAEQYPLPVVTLKEVFTSELDDTRKLIYSLEDGHIVEVVLMHYHYGWTICTP
                     SQVGCAMGCGFCASTLDGKIRNLDRAELLSIFYKSSAYIGEIGRVVLMGSGEPLDNYD
                     EVLGYLRLLTDERGRNLSARHITLSTCGLIDQIHSLANENLPITLAISLHAPNDRIRR
                     KIMPIANKFPVNDLIKAGQYYQDQTGRRVSMEYTLIEGVNDSLSHAVELANKLKGRGF
                     HVNLISVNPLEERDYSSPLKNVVEQFKIELEARNINVTIRRELGNDISGSCGQLRNRH
                     VKLEKRR"
     gene            2841..3251
                     /locus_tag="VFD08_02140"
     CDS             2841..3251
                     /locus_tag="VFD08_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09849.1"
                     /translation="MVKIIGGGKGSGKTKRMIRMANEVAVDPHGSLFFIDNDNRNIFE
                     LNRNIRFINKDEFKINTPERLYGFLCGILAGDYDTEKIYIDGIDYIGNVENTVLNEFL
                     EKLNKLALDNEAEFVLCFTTEEGQLSSFPQDRIL"
     gene            complement(3305..3994)
                     /locus_tag="VFD08_02145"
     CDS             complement(3305..3994)
                     /locus_tag="VFD08_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HZK09850.1"
                     /translation="MNYQKKMNYSYSLGAYPTIELCTKKPEFVRVIYLHPKISSELKR
                     KILSIAREHSLEYLESPTFFEKLEKENIYLAAKFTKFTSTLAQGNHLILHKPEMIGNL
                     GTILRSSIAFGLRDIAIIDPSCDEYHPHCIRASMGAIFSLRIEHFSCTEDYMKKYPQN
                     IYAFMTGEHTLLKKQTFKSPWSLLFGSESSGLPLVYQERFHPVKIKMSPDVDSLNLPL
                     AVGLALYHLYE"
     gene            4053..4961
                     /locus_tag="VFD08_02150"
     CDS             4053..4961
                     /locus_tag="VFD08_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HZK09851.1"
                     /translation="MPYYKLSESLINKYGVKTYKLPLHVEGTCPNRDGSISTGGCNFC
                     AEGGMDFELLESKIPIVQQLEKNKAYIGKRYGAKAFIAYFQNYSATYRPTKELISMME
                     QCLIDDVVEISLSTRPDCLPSTMLDEIKNFSYKHGVDVTIELGVQCLNDKVLVDMARG
                     HDAKTSLSAMEQVKKRDLGLGVHCILNYPGMEESDVIDLAKACSDFGADRVKLHSLYI
                     EKGTRLGDDFLEGKITLCDSDEYLERVLSFLAYLKESIAVERFFARAPEESSLFCNWS
                     RSWRYLMKELEDMMESRNFKQGQRRR"
     gene            4964..5761
                     /locus_tag="VFD08_02155"
     CDS             4964..5761
                     /locus_tag="VFD08_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HZK09852.1"
                     /translation="MYNVREWNRFLIPYEQAVEELKIKFKSLRNGYREVGEYSPIEFV
                     TGRVKEVSSILEKSKKLDIAYDDIQAKMEDIAGIRIMCQLIDDIYDVVDFINDRDGKD
                     FTVLYAKDYIKNPKESGYRSFHLVIKYPVYTAFGFTEILAEIQIRTLAMNFWAIIEHS
                     LNYKYRKQIPGKIAERLTRAAESVWALDAEMLAIKEEITSAQVLFEVNSDAVQSITTQ
                     IQLMNAGGEKDLAAMYQKKLDNTLDLSSSDALEALLMEMNSHLEEIS"
     gene            5758..6456
                     /locus_tag="VFD08_02160"
     CDS             5758..6456
                     /locus_tag="VFD08_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZK09853.1"
                     /translation="MKIFALGDLHLGFAVNKPMDLFGPIWKDHHKKIKDDWYARVKNE
                     DLVIIAGDTSWAMYYEDAKVDLEWIDALPGTKVIIKGNHDYWWQSLTKMQSYESIFFL
                     HNNYYKAGDIAICGTRGWVLPGNDEFGMDDEKIYHREKLRLERSLDLAKKDPEIETII
                     VAMHYPPFYKYQKETYFSELLKDPMIKHVVYGHLHDKDSWSRAVQGLYDGSEYYLVSA
                     DYKNFKLEQLNIND"
     gene            6449..7105
                     /locus_tag="VFD08_02165"
     CDS             6449..7105
                     /locus_tag="VFD08_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZK09854.1"
                     /translation="MIDLKGIKGIIFDFDGTLLDSLGMWKNLDIEYLNQRGISKPADL
                     GHAIEGLSFNETASYFKNRFSIEESVDEIVSHWHTYIEEVYPSLPFKASAKNFIERRK
                     KEGYKLAIATSNSKPLVKMALEDKMMERFFPIIVTSDDVGEGKPSPNVFLETAKRLQI
                     HPSDCLVIEDTYMGVLGAKRAGMKTIAIFDSHNKERWDKTLKLADGTVTHFKQLEAMF
                     "
BASE COUNT         2420 a         1192 c         1605 g         2252 t
ORIGIN      
        1 gaaaatactc ctgccataat tcatggaggt ccttttgcaa acatcgccca tgggtgcaat
       61 tctgttttag caacagaact tggacttttg acatcggata ttacggtaac agaagctggc
      121 tttggtgctg atttaggggc ggaaaagttt tttaatatca agtgtcgctc tgcaaaacta
      181 aatcctgatg ctgtcgttct tgtagcaacc attcgtgctc ttaaaatgca tggaggcaaa
      241 gccaaaaacg aattagctaa tgaagattta ggagccttaa agaaaggtgt tgtgaatcta
      301 gaaaaacaca tggaaaactt agctctattt ggggtacctt ttatggtggc catcaatacc
      361 ttcccaacgg atacggaagc ggagctttct tggttatttg attatgtaaa agatcttggt
      421 gttcccatag caaggagtga agtttttgct aaaggaggcc aaggtggcat ggaaatggca
      481 aaaacgcttc tagaacttat ggaaaaagaa gaaagccatt tcacacctct ttatgacaat
      541 gaaatgccta ttaaggaaaa aattgaaacc attgctaaaa aaatctatgg agcttctgat
      601 gtgcattatg acaagagtgc ccttaaacag ctaaaagata ttaaagatat aggtcttgat
      661 catctacctg tttgtatggc taagacgcaa tattctttta gtgatgatcc cactcttctt
      721 ggaagaccag aagattttga cctaaccatt cgcgaactcc gcattagtgc tggtgctggc
      781 tttattgttg cacttacggg tgcagtaatg accatgccag gtttgcccaa acggcctgcg
      841 gcattaaata tggatgttct agatgatggg gaagtggtag ggctatttta atgaaggtac
      901 tttattgcac tcctatagag ccagaaggag aaaaacggat tcaagatctt ggttataagg
      961 ttgttgctaa aaaagaaaaa gatttaaggc aagaagacct tgaaaatgtt gatgtttttg
     1021 taggtatgaa ccccttttct aaaatagact ttacaaaggg aagccttaaa tttattcagc
     1081 ttcaaaccca aggttttgac catttgccta aaaacgacct tgagggagtc cttgtagcta
     1141 ataatcatgg aggctatgcc atccctattg gtgagtggat tgtaacaaag atattagaaa
     1201 gttttaagca tacacgcaat atctacaatt tagaaaaaaa acgtcagtgg aaaaaggatt
     1261 ttggtataga ggagcttact ggtaaaaagg ttctttttgt tggaacagga accatagcca
     1321 gtgaagcagc aaaaagactc ttaccctttg gtgtggaaat ttttggaaca aatcgttcag
     1381 gaaaaagtgc gccatttatt gagaagatcc taaagctaga tgagcttgat gagattcttc
     1441 ctcaagtgga tgttcttgtt cctgttttgc cagcaacaaa agacactgag gatctaattg
     1501 atgcttctat gctaaaggcc ttaaaagcgg gttcagtgat tgtcaatatt tcccgggggc
     1561 aaattataga tgaaaaagcc cttctagaag ttattgataa gtttctagct gttcatttgg
     1621 atgtaactaa gcaagaaccc cttcctgtgg atcatccttt atgggaaaaa gaaaatgtct
     1681 atatttctgc ccatacatcc tgggttagcc agtatgttga taaaagacga gatgatctaa
     1741 tttatgaaaa tctaagacgc tttatacaag cagaagagct tcttaatctt atagacctaa
     1801 ggcgaggtta ttaatgaatt tactaggaat gtcctatgaa gaagttatga agattgattt
     1861 taaccagcct aactatagaa ataagcagat ttttgaaggc cttcataagc aggggataga
     1921 ctatgaaaaa atgagctccc tccctcttaa gctaagaaaa caactggcag agcagtaccc
     1981 gctgccagtt gtaacactta aagaagtttt taccagtgag cttgatgata ctagaaagct
     2041 aatttattct ttagaagatg gccatatcgt tgaggttgtt cttatgcact accactacgg
     2101 ttggacgatt tgtactccaa gtcaagtcgg ttgtgccatg ggctgtggtt tttgtgcctc
     2161 aacactagat ggcaaaatcc gcaatcttga ccgcgctgaa cttttgagta ttttctataa
     2221 aagcagtgcc tatattggag agattggtcg tgtagtttta atgggcagtg gtgaaccttt
     2281 agataattat gatgaagtcc taggctattt gaggctgctt actgatgaga gagggcgcaa
     2341 tctttctgca agacatatta ctctttctac ctgcggtctg attgatcaaa ttcactcctt
     2401 agcaaatgaa aacctaccta taactctagc gattagcctc catgctccca atgatcgaat
     2461 acgtagaaaa attatgccta ttgctaataa atttcccgta aacgatctaa taaaagcagg
     2521 ccagtattat caggaccaaa cgggtcgaag agtaagtatg gaatatactt tgatagaagg
     2581 ggtaaatgac agcttaagtc atgcggtaga actagcaaat aagttaaagg gccgaggctt
     2641 tcatgtgaat ttgatttctg tgaatccact agaagaaaga gattattcct ctcctcttaa
     2701 aaatgttgta gaacaattta aaatagagct tgaagcacga aatattaatg ttacaattag
     2761 aagagaatta ggaaacgaca ttagtggctc ttgtggtcag cttcgcaatc gtcatgtgaa
     2821 gcttgaaaaa aggaggtaaa atggtaaaga taattggtgg tggaaaaggt tcaggaaaga
     2881 caaagcgaat gatacgaatg gctaatgagg ttgcagtaga tccccatgga agtctgtttt
     2941 ttatcgataa tgataatcgt aatatctttg aactgaatcg taatattcgt tttatcaata
     3001 aagatgagtt taaaataaat actccagaac gtctttatgg attcctttgt ggaattttag
     3061 ctggagatta tgatacagaa aagatttaca ttgatgggat agattatatt gggaatgtag
     3121 agaacacagt acttaatgaa tttttagaaa aacttaataa attagcgctt gataatgaag
     3181 ctgagtttgt tctttgtttt actactgaag aaggacaatt gagttctttt ccccaagata
     3241 gaatacttta aagtttagag gcctccaata ttggaggcct tataacataa gtatttaaag
     3301 gcttttattc ataaagatga taaagagcaa ggcccacagc caagggtaga tttaatgagt
     3361 ccacatcagg actcatttta atttttacag ggtgaaaacg ttcttggtag acaagaggta
     3421 ggccagatga ttctgagcca aatagaaggc tccaggggct cttaaaggtt tgctttttta
     3481 gcagggtgtg ttctcctgtc ataaaagcat agatattctg cggatatttt ttcatatagt
     3541 cttctgtgca agaaaaatgt tctatacgaa gagaaaaaat tgcccccatg gaagctctaa
     3601 tacaatgggg atgatactca tcacagcttg ggtcaataat ggctatatct ctaagtccaa
     3661 aagcaatgga actccttaaa attgttccaa gatttccaat catttctggt ttgtgaagaa
     3721 taaggtgatt tccctgggct aaagtagaag taaatttcgt gaatttagcg gctaagtata
     3781 tattttcttt ttcaagtttt tcgaaaaagg ttggtgattc aagatattct agggagtgct
     3841 ccctcgctat agaaaggatt tttcttttca gctcactgct gatttttgga tgcagataaa
     3901 ttacccgaac aaattcaggc ttttttgtgc ataattctat tgttggataa gctcccaaac
     3961 tataggagta gttcatcttt ttttggtagt tcacttttac ctccgtgcaa aatgctaata
     4021 gtattgtata atgaactttg aggaaggaca agatgcctta ttataaatta tcagaaagtt
     4081 taataaataa atatggtgta aaaacttata agctccccct tcatgtcgag gggacctgtc
     4141 ccaacaggga tggaagtatc tccacaggag ggtgtaattt ttgtgctgaa ggtgggatgg
     4201 actttgaatt attagagtca aaaatcccca ttgtccagca gctagaaaaa aacaaggcct
     4261 atataggaaa acgttacggg gccaaagctt ttattgccta ttttcaaaac tatagcgcca
     4321 cctaccggcc caccaaggag cttatctcca tgatggagca gtgtttaata gatgatgtgg
     4381 tggagataag cctttcaact aggcccgatt gcctgccctc tactatgctt gatgaaatta
     4441 aaaactttag ttataaacat ggggtagatg tgacaattga gcttggtgtg cagtgcctta
     4501 atgataaggt ccttgtagac atggctcgtg gccatgatgc aaaaacatcc ttaagtgcaa
     4561 tggagcaagt aaaaaaaagg gatttaggtc tgggagttca ttgtatcttg aattatccgg
     4621 gtatggaaga aagtgatgta atagaccttg ccaaggcctg ttctgatttt ggtgctgacc
     4681 gagtaaagct tcattctctt tatatagaga aaggaaccag attgggcgat gacttcttag
     4741 agggtaaaat cactctttgt gattcagatg aatatctaga aagggttctt agctttttag
     4801 cctatttaaa agaatccatc gctgtggaaa ggttttttgc ccgcgctccc gaagaaagca
     4861 gtttgttttg taattggtct agaagttggc gttatttgat gaaagaatta gaagatatga
     4921 tggaaagtag aaattttaag caaggtcagc gcaggaggta aatatgtata atgtcagaga
     4981 atggaaccgc ttcctaattc cctatgagca ggctgtggaa gaattaaaaa taaaatttaa
     5041 atcacttcgt aacggatata gagaagtggg ggagtattcc cctattgaat ttgttactgg
     5101 acgtgttaaa gaggtatcta gtatcttaga aaaaagcaag aaactagata ttgcttatga
     5161 tgatatacag gccaaaatgg aagatattgc tggtatacgt ataatgtgcc agctgattga
     5221 tgacatttat gatgtggtag attttattaa cgatcgagat ggtaaggatt ttacggttct
     5281 ttatgcaaaa gattatatta aaaaccccaa agaaagcggc tatcgctctt ttcatttggt
     5341 gattaaatat cctgtatata ctgcttttgg ttttactgaa attttagcag aaatccagat
     5401 acggaccttg gcaatgaatt tctgggctat tatcgaacat tctttgaatt ataaataccg
     5461 aaaacaaatt cctggaaaaa tagcagagcg tttaaccaga gctgctgagt cagtctgggc
     5521 actggatgct gaaatgcttg caattaaaga agagattact tcagcccaag ttctatttga
     5581 agtaaatagt gatgcagttc aatctataac aactcaaatt caacttatga atgctggcgg
     5641 tgagaaggat ttagccgcca tgtatcagaa gaaacttgat aacaccttag atttgtcttc
     5701 tagcgatgcg ctagaagcgc ttcttatgga aatgaatagt catctggagg agatttcatg
     5761 aaaatatttg ctctaggtga tcttcatcta ggttttgctg tgaataaacc tatggatcta
     5821 tttggtccga tttggaaaga tcatcataaa aaaattaaag acgactggta tgccagggta
     5881 aaaaatgaag atttagttat tattgctggc gatacctcct gggccatgta ttatgaagat
     5941 gcaaaggtag atcttgagtg gatagacgcc ttgcccggta caaaggttat tatcaaagga
     6001 aaccacgact actggtggca atctctaaca aaaatgcaga gttatgagag tattttcttt
     6061 cttcataata attattataa ggcaggagat atagctatct gtggaactcg aggctgggta
     6121 cttcctggaa atgatgagtt tggcatggac gatgagaaga tatatcatag agaaaaactt
     6181 cgattagaaa gatcccttga tttagccaaa aaagatccag agattgaaac catcatcgta
     6241 gcaatgcact atcctccctt ttacaagtat caaaaagaga cgtatttttc cgaactttta
     6301 aaagatccca tgataaaaca tgtagtctat ggccatcttc atgataagga ttcttggtct
     6361 cgggctgtac agggccttta tgatggaagt gagtattatt tggtgtcagc ggattataaa
     6421 aactttaaat tggagcaatt aaatattaat gattgattta aaaggtatta aaggaataat
     6481 ttttgatttt gatggtacgc tacttgattc cttgggaatg tggaaaaacc ttgatattga
     6541 atatttaaat caaaggggca tatcaaaacc ggcagatcta ggccatgcca ttgaaggttt
     6601 aagctttaat gaaacagcaa gttattttaa aaatcgtttt tctattgaag aaagtgtaga
     6661 tgaaattgtt agccattggc acacctatat agaagaggtt tatccatccc ttccatttaa
     6721 agccagtgcc aaaaacttta tagaaagacg aaaaaaagaa ggctacaagc ttgctatagc
     6781 aacatcaaac tctaagccct tggttaagat ggccttagag gataaaatga tggaaaggtt
     6841 ttttcccatt atcgttacct cagatgatgt gggggaagga aagccatctc ctaatgtgtt
     6901 tttagaaaca gcaaaacgct tgcaaattca tccttcagac tgtcttgtga ttgaagacac
     6961 ctatatgggc gttttaggag ccaaaagagc gggaatgaaa accattgcca tttttgattc
     7021 tcataataaa gaaagatggg ataaaacttt gaagctagct gatgggacag tgactcattt
     7081 taagcaacta gaagcaatgt tttaagagtt atttagtctg ttttgtggag actttattaa
     7141 cttgtgatat tgtatctaat aatacccaaa cttgtcttgt gtccagatat ccctaaagat
     7201 tcttatccct agggtcttta tagaaaatga aaacaagaca tttaagtgct gattagatgg
     7261 aggtctacct tgccaataaa ggataataat atgagcctat atagtatgac aggatatggt
     7321 gtatccagac gtaaaattga caaaaccgaa gtgaaagtag agatgaggtc tgttaactct
     7381 cgatttttag aaataaatat gcccatgcca agaaagcttc ggccctatga aattgatctt
     7441 cagaatcttc ttaaaaagaa gcttaatag
//
LOCUS       DASRNA010000077         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_921864,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000077 DASRNA010000000
VERSION     DASRNA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921864"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..785
                     /locus_tag="VFD08_02170"
     CDS             54..785
                     /locus_tag="VFD08_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZK09855.1"
                     /translation="MEDYIIEVEDMTVAYDVKPVLWDVDLQIPRAVLMAIVGPNGAGK
                     STLIKAMLDLIKPVSGKVSFNGASYKSQMKHIGYVPQSESVDWDFPTNVLDVVTMGLY
                     GKLGWFKRPGKEEKSQSLEALDKVGMKEFANRQISELSGGQQQRVFLARALVQDADIY
                     FMDEPLKGVDAKTEKAIIDLLKELKNRGKTLIVVHHDLQTVEEYFDHVVLLNTQIIAS
                     GPVKETFTDENLEKTYRSTGKILKR"
     gene            795..1670
                     /locus_tag="VFD08_02175"
                     /pseudo
     CDS             795..1670
                     /locus_tag="VFD08_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            1928..3076
                     /locus_tag="VFD08_02180"
     CDS             1928..3076
                     /locus_tag="VFD08_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZK09856.1"
                     /translation="MSPEIEIQLIAVIVAIACSLPGVFLVLRKMAMMTDAITHTILLG
                     IVIAFFATQDLSSPLLILGAALMGVFTVFLTEILHSTKLVSEDSAIGVVFPLLFSIAI
                     IIISKYAGSIHLDTDAVLLGELAFAPFNRMVVFGRDIGAKAIYSMGIILVINILYIVI
                     FFKELKIVTFDPALSAVLGFMPALMHYSLMSIVSITAVGAFESVGSILVIAFMIGPPI
                     TAYLLTDDLKRMILLIAFIGAANGILGYQIAKYFDVSIAGSMALMTGISFFLVFVFAP
                     KRGLITTVSRRKKQRIEFAGNSLLFHIMNHEGEEDEALENGLATINEHLNWEEDFLKH
                     ILSDLKEREKIQIEEGIIKLTDLGRIYTAESYENIVCGPEGCPVKMIG"
BASE COUNT         1065 a          556 c          699 g          962 t
ORIGIN      
        1 tatagatacc atagtagatg ccttaaaata aattttataa aaaggggaga tagatggagg
       61 attatataat agaagtagaa gatatgacag ttgcctatga tgtaaagcct gtactttggg
      121 acgttgacct acaaattcca agggctgttt tgatggccat agtagggcca aatggagcag
      181 gcaaatcgac acttattaag gccatgctag atcttattaa acctgtatcc gggaaagttt
      241 catttaatgg agccagctat aaatcgcaaa tgaagcatat tggctacgtt cctcaaagcg
      301 aaagtgtaga ctgggacttt ccaacaaatg tactagatgt tgttacaatg ggactatatg
      361 gaaagctagg atggtttaaa agaccaggaa aagaagaaaa gagccaatcc ctagaggcac
      421 tggacaaggt gggaatgaaa gaatttgcca atagacaaat ttcagaatta tcaggaggcc
      481 agcagcaaag agtttttcta gcaagggcct tggtgcaaga tgcagatatc tattttatgg
      541 atgagcccct taaaggtgtt gatgccaaga cagaaaaagc cattattgat ttattaaaag
      601 aactaaaaaa cagaggaaag actcttattg tagtccatca tgaccttcaa acagtggaag
      661 agtattttga tcacgtggtt cttttgaata cacaaataat agcaagtggc ccagttaaag
      721 agaccttcac cgacgaaaac ttggaaaaaa catatagaag cacaggaaaa atattgaaaa
      781 gataggtggt tgctttggat atattgcaaa ttgtttttac agattatacc tttagggtag
      841 tttccctagg agccatgcta cttggaatca tatctggagt actaggaagc tttgcagtca
      901 taagaaagca aagtttacta ggagatgcag tttctcatgc tgccctgcca ggtatagcct
      961 tggcatttct atttacctta accaagagga cagaagtgct tttgttggga gccctaattt
     1021 caggcttatt ggcgacttgg ttgatagtta tgatagatag atattcaaga ataaaatttg
     1081 atagcgccct agcccttatt ttatcggtgt tttttggtat cggtatggta cttttaacct
     1141 atatacagag gattccaagc tcaaatcagg ctgggctaga aaagtttata tttggccagg
     1201 cttccactct tttaaaaaga gatgtttata taatgttgat actaggggtc atactgcttt
     1261 ctatagttgt tttgttttgg aaagaattta aacttgtctc ctttgatgag gagtttgcta
     1321 ggagtcttgg ctactcaacc aggctgatta cttccctctt atttctacta attgttacag
     1381 ccattataat tggacttcaa acagtggggg taatactaat gagcgccatg ctggtagccc
     1441 ccgctgtagc ggccagacaa tggacagaca agctatctct tatggtcttt ttatcagccc
     1501 tatttggtgc catatcggga gtggtcggta ccatcttaag ctcaacaata gcaaaactcc
     1561 caacagggcc cactatagta gttgttataa gcactatcgt tcttataagc cttacctttg
     1621 cacccaatag gggactggta tggaattact ttaggcaaag aaaaagcaaa agagaaataa
     1681 atgaagacca attgctggct aatttatatg atttagccat gaaccatgat gatatatccc
     1741 atggccatga tgtatatatc attataccta aaacagctgg tgatagggtt tctaaaagaa
     1801 atgcgatcaa taaactagag tctttagagg ctagaggatt ggcaaaatca gatcaagtgg
     1861 gcaaatggtc aatcacagaa aaaggcttag attatataaa aaagtatagt gaaaggagtg
     1921 atggctaatg tcacctgaaa ttgaaattca attgattgca gtaattgttg caatagcatg
     1981 ttcacttcct ggagtgtttt tagtattaag aaaaatggca atgatgaccg atgcaattac
     2041 tcatacaatc cttctaggta tagtaatagc attttttgca acccaggatt tatcctctcc
     2101 acttttaatc cttggagcag cactaatggg agtatttact gtctttttga ctgagatctt
     2161 acacagcacc aagctagtat ctgaagattc agcaataggg gtcgtatttc ccctgctctt
     2221 tagtatagct attattatca tatctaagta tgctggatcc atccacctag atactgatgc
     2281 cgtcctacta ggagaactgg cctttgctcc atttaataga atggtggtct ttggtagaga
     2341 tataggggcc aaggccatct attctatggg gattatactg gtaatcaaca tcctatatat
     2401 agtgattttc tttaaagaac taaaaatagt gacctttgat ccagccctta gtgcggtgct
     2461 gggctttatg cctgctctta tgcactactc acttatgagc atagtgtcta taacagctgt
     2521 aggagccttt gaatcagtgg ggtccatcct ggttatagcc tttatgatag gcccccccat
     2581 tacagcctat ttactaacgg atgacttaaa gaggatgatc ctgcttatcg cctttattgg
     2641 tgcagcaaat gggatacttg gataccaaat agcaaaatac tttgatgtat caatagcagg
     2701 gtcaatggcg cttatgacgg gcattagttt ctttttggta tttgtatttg ccccaaaaag
     2761 agggcttatc accaccgtca gtagaagaaa aaaacaaaga atagaatttg cgggaaactc
     2821 tcttttattt catataatga accatgaagg cgaggaagac gaagctcttg aaaatggact
     2881 agcaacaatc aatgaacacc taaactggga ggaagatttc cttaagcaca ttttaagtga
     2941 tttaaaagaa agagaaaaaa tacaaatcga agaaggaatc atcaagctta cagatttggg
     3001 cagaatctat acagcagaaa gttatgaaaa cattgtttgt ggacccgagg gatgtcctgt
     3061 gaaaatgatt ggataataag gtccaacgat aaggactaag ctagagcaaa accgatgatt
     3121 tcttatcagc ctgggggcca aggatgacgg atatttgctt attttgtagg atttataaaa
     3181 aagcttagaa aaagaatatc taaaaatttt aaaaaaacag ctcttgactt atgagcgaaa
     3241 aaaatatata ttagctagta aggtctttag gccttattat at
//
LOCUS       DASRNA010000078         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_543421,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000078 DASRNA010000000
VERSION     DASRNA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543421"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFD08_02185"
     CDS             complement(<1..625)
                     /locus_tag="VFD08_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK09857.1"
                     /translation="MTDYKYVYGPVPSRRMGLSVGISPIPKGYCNYSCIYCQLGRTSH
                     MTNKRENFFKQEDIVEEFKDYISKGTHFDVVTIVGEGEPSLYLDLGILIEKLKELTDK
                     PLAVITNGALLSDAGVRKNLKAADIILPSLDASNEEIFKKINRPHKNINFDSLLQGLE
                     TFSQDYKGELWLELMILRDINDSKEFFLDYKKLVEKINYHKLYINSPV"
     gene            complement(758..928)
                     /locus_tag="VFD08_02190"
     CDS             complement(758..928)
                     /locus_tag="VFD08_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09858.1"
                     /translation="MPKKDVGGRGRMGGPASAGPQGYCKCPSCGEKIKHKPGLPCTSR
                     SCPKCGTKMLRD"
     gene            complement(969..1625)
                     /locus_tag="VFD08_02195"
     CDS             complement(969..1625)
                     /locus_tag="VFD08_02195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK09859.1"
                     /translation="MTKGKKTSTCLLCDGQTKLFYQSKSKHYYKCENCLALMLDPGDY
                     LNSQEEKERYETHNNDIDDIRYQSFVSPIVESVRASYGTNHLGLDYGAGTGPVITSLL
                     EKEGYQLKLYDPYFHYYPENLLKKYDYIVCCEVIEHFHKPYKEFASLSNMLKPGGTIF
                     SMTEIYHEGIDFETWYYKNDDTHVFFYHKKSLEWIKKEFGFAHLEIEGRLIKLKKEHL
                     "
     gene            1857..2735
                     /locus_tag="VFD08_02200"
     CDS             1857..2735
                     /locus_tag="VFD08_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZK09860.1"
                     /translation="MSELRPIELKYSIEKEHWVGSAFRVRNYFPEGKNLLKRFSPFVL
                     LDYNAPKVFPPSKTPKGIGPHPHKGIETVTFAFKGSIEHHDNAGNHGIIHPGDVQWMS
                     AGSGIMHKEYHEKNFQKKGGSLQMVQLWIKLPKKDTLAAPRYQEIKKEKMGRITLADK
                     KGRLLVVAGNYKQVKGPAKTFSPINIFIIDLEVGASFLLNQPADYNTGLLILSGNLTI
                     NSESCQENDFVLFENKKGDIVMDHITSASKLLLLSGQALNEPLLAGGPFVMNTKEELD
                     QAFKDYREKKFGREDF"
     gene            2908..>3308
                     /locus_tag="VFD08_02205"
     CDS             2908..>3308
                     /locus_tag="VFD08_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK09861.1"
                     /translation="MSKFTTSDKVEIVYTVKGEGKPLVLIHGWSQSKEAFAPQLEELS
                     KKYKVVAYDLRGHGESERTQRGLTLDTLARDLKELIEHLNLDKVLLAGWSMGASTIFN
                     YVKDYGVKYLAGIVLFDMTPKLLNDQEWNLG"
BASE COUNT          983 a          678 c          610 g         1037 t
ORIGIN      
        1 taacagggct attgatatac aatttatgat agtttatttt ttccactagt tttttatagt
       61 ccagaaaaaa ctctttgcta tcatttatat cccttaaaat cataagttca agccataatt
      121 ctcccttata gtcctgggaa aaagtctcca gtccctggag caggctatca aagtttatgt
      181 ttttatgggg ccgatttatt tttttaaaga tttcttcatt actggcatcc agggagggaa
      241 gaatgatatc agctgccttg aggttttttc tcactccagc atcagataat aaggccccat
      301 ttgtaataac cgccagaggc ttatctgtaa gctcctttag tttttcaatc aagatcccta
      361 gatctaggta taggcttggc tctccttcgc ctactatagt aaccacatca aaatgagtgc
      421 ccttgcttat atagtcttta aattcctcaa caatatcttc ctgcttaaag aagttttctc
      481 ttttgtttgt catatgacta gttcttccca gctggcagta tatacaggaa taattacagt
      541 atcctttggg tatggggcta atccccacag aaagtcccat cctcctagag ggaacaggtc
      601 catatacata tttgtagtct gtcatctgct tcactcctaa acttgacttt attaattctt
      661 caaggataaa agtgtacctc tatagttgta atattggtac actttcattt tttagtaaag
      721 tcactggtct gctttaagtg gtttttaccc tgccctctta gtctcttagc atttttgtgc
      781 cacattttgg acagcttcga cttgtacaag gtagtcctgg cttatgcttt attttctcac
      841 cacagctagg acacttgcag tagccctggg gccccgcact tgctggacct cccattctgc
      901 ctcttcctcc tacatctttt tttggcatgg tttcacctcc tttaaatata aacttactta
      961 gtcctttatt ataagtgctc tttttttaat ttgattaacc taccctcaat ctcaaggtgg
     1021 gcaaagccaa attccttttt aatccactct aaggattttt tatgataaaa aaacacatgg
     1081 gtgtcatcat tcttataata ccaagtctca aaatcaattc cttcatgata gatttctgtc
     1141 atagaaaata tagtcccccc cggctttagc atatttgata aggaggcaaa ttctttatag
     1201 ggcttatgga aatgttctat cacttcgcag caaacaatat aatcatattt cttcaataga
     1261 ttttctggat aataatgaaa gtatgggtca tatagtttta gctggtagcc ttctttttct
     1321 aaaagactgg ttataacagg ccctgtgcct gctccatagt caagtccaag atgatttgta
     1381 ccatagctag ctcttacact ttctacaata ggagaaacaa aactctgata gcggatatca
     1441 tcaatgtcat tattatgggt ttcatatcgc tctttttctt cttggctgtt taagtagtcc
     1501 ccggggtcta acattagggc caggcagttt tcacatttat aatagtgctt actcttactc
     1561 tgataaaaga gcttggtctg gccatcacaa agcaaacacg tagaagtttt tttcccctta
     1621 gtcacaatct ttctcctctt tattttctaa gatcctttag gacctctttt tatagtttct
     1681 ttagcttttt tatttaatcc cttcctatct aatacaagta tcccacttta ggggggctcc
     1741 gtcaaaattt atttcaaaac acacaggagg gagcctctat agggcagcta tctttgttag
     1801 taaagttctt tcaagggtat cttataagta agccatataa gaagtgaggt gacaagatgt
     1861 cagaacttag acctatagag cttaagtatt ctattgaaaa agaacactgg gttggcagtg
     1921 cctttagggt aagaaattat ttccctgaag ggaaaaatct tcttaagaga ttttctccct
     1981 ttgttctctt agactataat gcccccaagg tatttcctcc ctctaagacg ccaaagggaa
     2041 tcggtcccca cccccataag gggattgaaa ccgttacctt tgcctttaaa ggctccattg
     2101 aacatcatga taacgctggt aaccatggca ttattcatcc cggggatgtc cagtggatga
     2161 gtgcgggttc aggtattatg cacaaagaat atcatgagaa aaactttcaa aaaaaaggcg
     2221 gaagcctcca gatggttcaa ctttggatta agctgcccaa aaaagataca ctagcagcac
     2281 caagatatca ggaaatcaaa aaagaaaaga tggggagaat taccttggcc gataaaaaag
     2341 gaaggctgct ggtggttgcg ggaaactata agcaagtaaa aggaccggct aaaactttct
     2401 cccccataaa tatctttatt atcgacttag aagtcggtgc cagcttcctt cttaatcagc
     2461 cagctgatta caatacaggg ctccttatcc tctcgggaaa tctaactatt aatagtgaaa
     2521 gctgccaaga aaatgacttt gtcttatttg aaaataaaaa gggagatata gtcatggatc
     2581 atatcactag tgcttctaag ctgctgcttc ttagtggtca agcccttaat gaacccctgc
     2641 tagcaggagg cccctttgta atgaatacca aagaggagct tgaccaggcc tttaaagatt
     2701 accgggaaaa aaagtttggc agagaggatt tttaacaagt tgctagcaag cccctagaca
     2761 aggattataa caataaatgc atcaacttct aatctacgct tcatttatgg cctgaatagt
     2821 gacctaggag cccaagctcc tttgataatt tgcaagagta aagatataat gaaactatat
     2881 tcaaacaaag ctaaaaggag gcttaatttg tctaaattta caacaagtga taaggtagaa
     2941 attgtttaca cagtaaaagg tgagggaaaa ccccttgttc tcattcacgg atggtcacag
     3001 tccaaagaag cttttgcccc ccagcttgag gaactaagta aaaaatacaa ggtagttgcc
     3061 tatgacttaa ggggtcacgg agagtctgag agaacacaaa ggggactaac cctagacaca
     3121 ctggctagag atctaaaaga actaatcgaa catctaaacc tagacaaggt ccttcttgcc
     3181 ggttggtcca tgggagcctc aaccatattt aattatgtaa aggactatgg ggttaagtat
     3241 ctagctggaa ttgtgctctt tgatatgaca ccaaaactac taaatgacca ggagtggaat
     3301 ctaggatt
//
LOCUS       DASRNA010000079         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_470425,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000079 DASRNA010000000
VERSION     DASRNA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470425"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFD08_02210"
     CDS             <1..716
                     /locus_tag="VFD08_02210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HZK09862.1"
                     /translation="PMACINFGEDMRHFFLDRLDEKGYILDPVDIHHMSKVLRLVIGE
                     ELQLAYGGNLYHGKIEQIDKKKIVVNIEGEISRPLYPKIHLYQALAKGKKMEHILQHG
                     TELGISKFGLLPTRYSDVKSVSQKKERYLRILKDAAKQSQAPCIPEIDFYESLEELPF
                     DEDEIFFCYELEKENKINLQEKGSVGIIIGPEGGFSQKEASFIKSKAQAVSLGERILR
                     TETAGLVATALILRELGVL"
     gene            716..1909
                     /gene="mtaB"
                     /locus_tag="VFD08_02215"
     CDS             716..1909
                     /gene="mtaB"
                     /locus_tag="VFD08_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZK09863.1"
                     /translation="MRIKFINLGCKVNLNETENLKRACHQEGYEIVSEGDPADLIVIN
                     SCSVTHISDRKTRQKISQAKREEALVAVMGCYVDLHEEIMVDLLIPNNSKDQALELIK
                     ELVGEGPRQNEEFVYDKTRAFLKVQDGCNQFCSYCIIPYARGRARSYSFERIKKELIS
                     YLDEGFKEIVLTGINLSFYGKDTLSSLGELLTEIKPILGDNRLRLGSLEPHVISEDFL
                     RTCMDLPGFCPHFHLSLQSGSDSVLERMNRHYSTKDFKERVDLIRSFLPLAAITTDII
                     VGFPEETEKEFNETMDFVRDIGFSHIHVFAYSPRQGTAAAKQIDLHPEVKKRRSKLLR
                     KLAKENKEKYLDLFINKEVEVLFEENSSGLSREYIHCYSQTKKENNSLEELKVLKAEN
                     DRLIV"
     gene            1919..2257
                     /locus_tag="VFD08_02220"
     CDS             1919..2257
                     /locus_tag="VFD08_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZK09864.1"
                     /translation="MDCLFCKIVEGTIPSDKVYEDEHVIGFKDINPTSPLHLLFIPKV
                     HFEDLSHSYGKEEELLYVFKAIRKYVIEEGLDEKGYRLVINAGEYGQQTVAHLHVHLI
                     TGRQLNWPAG"
BASE COUNT         1020 a          506 c          657 g          886 t
ORIGIN      
        1 ggccaatggc atgtattaat tttggagaag atatgagaca tttctttctt gatagactag
       61 atgaaaaagg atatatttta gatccagtag acattcacca tatgagtaag gtgctaagac
      121 tagtaatagg cgaggagcta cagctagcct atggggggaa tctctaccat gggaaaattg
      181 agcaaatcga caaaaagaaa atagtagtaa atattgaagg ggaaatatcc aggcccttat
      241 atccaaaaat tcatctttac caagcccttg ctaaaggaaa aaagatggaa catatactac
      301 aacatggcac agaactaggc attagcaagt ttggacttct tcccacacgc tacagcgatg
      361 tgaaatctgt gagccaaaaa aaagagcgct acctaagaat actcaaagat gcggccaagc
      421 agtcacaagc cccctgtatt cctgaaattg atttttatga aagcctagaa gaacttccct
      481 ttgatgaaga tgagatcttt ttttgctatg aattagaaaa agaaaataag atcaacctcc
      541 aagaaaaggg gagcgtggga attattatag gtccagaagg gggctttagc caaaaagagg
      601 cgagttttat taagagcaaa gcccaagcag ttagcctagg tgagagaatt cttcgtaccg
      661 aaaccgcagg acttgtagct actgcgctta tattaagaga gttgggagta ctataatgcg
      721 cattaaattc attaatttag gatgcaaagt aaatctaaat gaaacagaaa atttaaaaag
      781 agcctgccac caagaaggat acgagattgt aagtgaaggt gacccggctg atcttattgt
      841 tattaattct tgtagtgtca cccatatcag cgatagaaag accaggcaaa aaatatcaca
      901 agcaaaaaga gaagaagccc tagttgctgt aatgggatgt tatgttgatc ttcatgaaga
      961 aattatggtg gatttactta ttcctaataa tagcaaagac caggcccttg agctaattaa
     1021 agagctagtg ggggagggcc caagacagaa tgaagaattt gtatatgata aaacacgggc
     1081 ttttttaaag gttcaagatg gctgtaatca attttgtagt tattgcatta tcccctatgc
     1141 gcgaggtagg gctagaagct actcctttga gcgaataaaa aaagaactaa tatcctatct
     1201 tgatgaaggc tttaaagaaa ttgttttaac agggatcaat ctttcttttt atggtaaaga
     1261 taccctatct agtctagggg agctcttaac tgaaatcaaa ccaattttag gagacaatcg
     1321 gctccgtctt ggctctttgg agccccatgt cattagcgag gattttttaa gaacctgtat
     1381 ggacttaccg ggtttttgcc ctcactttca tctttccttg caatcgggaa gcgacagtgt
     1441 cttagagcga atgaatcgac attactcaac caaggatttt aaagagcgcg tggacctgat
     1501 tcgaagcttt ttacccctag ctgccataac aacagatata atcgttggtt ttccagaaga
     1561 aacagaaaaa gaatttaatg aaacgatgga ttttgttaga gatataggat ttagtcatat
     1621 tcatgtattt gcctattccc ctcgccaggg aacagcggct gcaaaacaaa ttgaccttca
     1681 tcctgaggtt aaaaaaaggc ggagcaagct acttaggaag ttagccaaag aaaataaaga
     1741 aaaatacttg gatcttttta ttaataaaga agtggaagtg ctctttgaag aaaacagtag
     1801 tggcctttcc agagagtaca ttcattgtta tagtcaaact aagaaggaaa ataatagttt
     1861 ggaagaactt aaagtcttaa aagcagaaaa tgacagactt attgtctagg aggtaaaaat
     1921 ggattgtttg ttttgcaaaa tcgtagaagg tactataccc tcggataaag tttatgagga
     1981 cgagcatgtt ataggtttta aggatattaa tccaacaagt cctctacatc ttctttttat
     2041 tcccaaggta cattttgaag atttaagtca ttcttacggg aaagaagaag aactacttta
     2101 tgtatttaaa gccattagaa agtatgtaat agaagaaggc cttgatgaaa aaggataccg
     2161 acttgtgatt aatgcgggag aatatggcca gcaaacagtg gctcatttgc atgttcattt
     2221 aattactgga cgccaactta attggcctgc aggctagttg agtaaaaaac ccctgtttag
     2281 gttagttgtc aatctaaacg caacactggg ttgaaaaaac ctgtgcttta tgagtacctt
     2341 gtcatttcaa ggtaaacggg acgtgtgttt tcattaataa ggtcttcttt tgacctgttt
     2401 tgtgttaatt taagcttgct ttcttcttta cctttccatt tttttgtaca acaaaatgga
     2461 gtccccttag cgaatggtca tttactaagg taaagtggac ccctaaatat aattcttaag
     2521 aacattatat tttaagataa gcaaacttac aactggaatg atggcctagt ttaacattca
     2581 tagcaatagg tcacatgctt tctcgtgtaa gagtccaaaa agctggtaga taaacggtct
     2641 ctgtctgtaa taaaaacagg caaagggctt cataaagcgc tctagtcgca gatgttcctc
     2701 gtagtcatca aagatcttat ttaggatttt gatgtagtta cagtctcttt tgtgaaaacg
     2761 gatagttgga gccattcgag ttcatggaaa aggatgtagg gtcatgcttc ttttctcgta
     2821 cacaaaattt ctcaaggtaa aggagagggg tttgtttcct aaggtaagtt gggcacttga
     2881 aaaagttggt gttgcgttta ctttggaaag ttaggtagat tttaagccaa agggaaaact
     2941 agccacccgt agggcttgcc cttgataaac taatgaataa atgctacaat gcccatcacg
     3001 aaggcgaaat attatagtgc tttgagtttg tcgccttcgg gtgaaaattt taaagcattt
     3061 atgaatggt
//
LOCUS       DASRNA010000080        10865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_103761,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000080 DASRNA010000000
VERSION     DASRNA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103761"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..805)
                     /locus_tag="VFD08_02225"
     CDS             complement(68..805)
                     /locus_tag="VFD08_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HZK09865.1"
                     /translation="MPGNWVHYYFAKDMAKDHKFTFKNDKAYYLGALGPDFLYYLPQQ
                     SRGQEVFTMFHEEKTREILCYVFDWIEKKELKAYLYGFLTHYALDSGSSFFIYSLQNE
                     GFDKYSVKMALDEAILRRRNLRPGGRSPFYPLINLGKELPKEIARFYQGAAKEVYDLE
                     LSSEEINLAYRRFVKLLKISSSSFSFLKVRRNWYDRAPEQLYSKETSEKLFKEFLGSY
                     ENSRRFFERLLTDQFPCAERNFHGDLL"
     gene            complement(932..2572)
                     /locus_tag="VFD08_02230"
     CDS             complement(932..2572)
                     /locus_tag="VFD08_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZK09866.1"
                     /translation="MREGSNFIRDIVIEDIKSGKHDYAITRFPPEPNGLLHIGHCKAL
                     TINFDIAREFNGFTYLRFDDTNPAKEDERFVKLIKEDIRWMGFEWKELRYASDYFEEM
                     YKRAHILINKGLAYIDDISPEEMRRMRGSLTRGGVESKNRNRPIEESRDLFTRMKKGE
                     FPEGSLTLRAKISMDHPNIIMRDPVIYRIVDEPHYRTGDDWHIYPMYDFAHPMEDYVE
                     KITHSLCSLEFENNRPLYDWYLEHTDVPYQPRQYEFAKLIIEGAIIGKRHIMQLVEKG
                     LIDGWDDPRLLTISGMRRRGYRPQGIKNFIRATGVSKENSVVEMAMLEHFIREDLKII
                     TPRVMAVLDPLKVVISNYTKESEQLTMNNNVENPQLGSREISLSREIYIEREDFSENP
                     PKGFKRLSPGKEVRLMGAYFIRCDEVIKDKEGKIIELVCSYDEKTKSGSGFKERKPQG
                     TIHWVDKKTSVPARVYRFGPLIEGVFDSSLELADQVAEGSVKEYQAQVEGVAGSSSNH
                     QAYQFIRHGYFAKDETHPEKLTFNETVSLKSSYRPRKK"
     gene            complement(2581..4275)
                     /locus_tag="VFD08_02235"
     CDS             complement(2581..4275)
                     /locus_tag="VFD08_02235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HZK09867.1"
                     /translation="MKFSQLVYQRPDLDQIENKAEELLSSFSEAESFKEHNEIFLQMI
                     EIEKDIESQGVLVHIRHSVDTLDSFYEKENDFWDENTARFTAFKHGLISAVLESPYLE
                     DYRELYGDHFIDRALLQKKTFKEEIMEDLVEESQWSSKYQKLMASVQIPFEGKERTLP
                     EMSVFLEDPDRDLRKGAGEAIFGFFQANLEHFDQIYDRLVKLRHTIALKLGYKNFVQL
                     GYDRMGRTDYGEQEVAAYRKGVRNFLVPLVEEIYQEQKKRLGLEKLYYYDLGYEYPGG
                     MPRPQGDSDFIISQAEKMYHELSAETSSFFKTLLDKELMDLLSKKGKAPGGYCTFIEN
                     YGLPFIFANFNGTIGDVNVLTHEFGHAFQVYSAKDLPLPSQRFPGMEAAEIHSMGMEF
                     ITWPWMENFFGEDSDKFFYSHAAGALSFIPYGVLVDHFQHEVYKNPQMTPQQRRAKWR
                     ELEKIYMPWKDYGGQPYLEEGGFWQKQAHIYEMPFYYIDYTLAQIVALQILRRSREDQ
                     KKLWEDYKAISRAGGSLPFLKILELGNFESPFDPKVVEETVAYLKDFLAGFDTSSF"
     gene            complement(4265..5533)
                     /gene="serS"
                     /locus_tag="VFD08_02240"
     CDS             complement(4265..5533)
                     /gene="serS"
                     /locus_tag="VFD08_02240"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZK09868.1"
                     /translation="MYDIKRIRRDFDEILRGVEKRGQGDFGLLEIRELEKKRREILVQ
                     VEQLRNEQKTKSRLIPAYKKEGKDPSELLEELRGLSERISGLDKELRQVEESFEEKLY
                     YVPNTPHKDIPLGKGEEDNKEIRRWGQPRVFEFEASPHWDLGVERNFLDFERGSKVVG
                     SRFTFIKGACARLERALMNYFLDFHTLDGKYTEIGSPGVANRLSMTGTGQLPKFIEDM
                     YHCAEDDYFLIPTAEVTLTNFHREEILPLEDLPVYITSFTQCYRREAGSAGRDTKGII
                     RQHQFGKVELVKLVEPESSFDELEDMVSDVEAILQSLELPYRVVELCSGDVGFSSAKT
                     YDLEVWFPSYDTYREISSCSNFLDFQARRCMLRYRNKEGKVDFLHTLNGSGIPTGRCL
                     AAILENNQTAEGKIIIPKVLRPYMGGLDEI"
     gene            complement(5791..6072)
                     /locus_tag="VFD08_02245"
     CDS             complement(5791..6072)
                     /locus_tag="VFD08_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09869.1"
                     /translation="MDAGEIPQGTSLDWVKVTRLLGEKFSSEEADLLFRLYGLEGYKP
                     HSAKELRKKTKLKKKAHHKKMLELQRKLFHLLKDKDPQGLFLAGEENDI"
     gene            complement(6036..6299)
                     /locus_tag="VFD08_02250"
     CDS             complement(6036..6299)
                     /locus_tag="VFD08_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09870.1"
                     /translation="MTQDIEIAQVSGVVLGCTHFLFLQKEIQNFFGPQVKIYDSHGET
                     LDLIQKNLGQGQGSGNYRIISSAGKKTEDKSRWMLEKYLKELP"
     gene            complement(6310..6792)
                     /locus_tag="VFD08_02255"
     CDS             complement(6310..6792)
                     /locus_tag="VFD08_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HZK09871.1"
                     /translation="MKKIGVYDSGMGGLSFLAKLIQEMPTYDYIYYGDALNVPYGEKK
                     KEEICSFARYVAKELIDLECEALVIACNTATSAAEEILKAEFDIPIFGIEPALSSAME
                     WGDRGKLLVMATNFTLSSPRYLLALERYQDQREILSLPGPALVELVEEGIVEGRKNSQ
                     "
     gene            6898..7089
                     /locus_tag="VFD08_02260"
     CDS             6898..7089
                     /locus_tag="VFD08_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZK09872.1"
                     /translation="MKPYNNDQVLERTENKYILSKVIAKRARQLKEEEDIAIGYMAIN
                     RAVQTLMEDDFSFKVHPKK"
     gene            7186..9801
                     /gene="ppdK"
                     /locus_tag="VFD08_02265"
     CDS             7186..9801
                     /gene="ppdK"
                     /locus_tag="VFD08_02265"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZK09873.1"
                     /translation="MTKFVYTFNEGNKDMKDLLGGKGANLAEMTNLGLPVPPGFIITT
                     QACNSFYDKGQIIWPQLKEEIFSHVKELEKQTGKSYGGENPLLVSVRSGGKFSMPGMM
                     DTVLNLGMNDATIPILIKESGNPRFVLDAYRRFIQMFGDVVKEVPKKKFDEVFDKVKK
                     DKGYSLDTQLEEEDLSLIVDAFKEIYKENTGSDFPQDPIVQLLESVEAVFSSWNNNRA
                     RVYREEHNISHKLGTGVTVQSMVFGNMGDDCGTGVAFTRDPATGEAKLFGEFLINAQG
                     EDVVAGIRTPMQIEEMNLINDHLYDDFVKICHTLENHYKDMQDIEFTIEKGTMYILQT
                     RTGKRTAHSALRIAVDLVEEGLIDKNQALLRVEPEQLDQVLHPTFDKKALREAVVIAK
                     GLNASPGAASGQIYFHAADAVKAAEEGIKPILVREETSPEDLEGMIKASGILTAKGGR
                     TSHAAVVARGMGKCCVAGCEALDVDEDKRQFSHKGKIYKEGDFISLDGSTGYVYEGVV
                     GTSAPILDENFKTLMDWADHVAHLTVRANADTPLDAKQALEFGARGIGLTRTEHMFFQ
                     EHRISAVREMILAQNAQDRVKALRKIMPYQKDDFVEIFKVMKGLPVTIRLLDPPLHEF
                     LPHRDIEIRALAKELDLSFEELKARVTALHEINPMLGHRGCRLAISYPEIAAMQTRAI
                     ILAAIEVKKEGIHVLPEIMVPLVGDKKELEYIEALVRPLAKELMQEHACEIPYSFGTM
                     IEVPRGALTAGEIAKSAEFFSFGTNDLTQMAYGFSRDDAAGFIDEYLEKGIFDMDPFV
                     SIDPKGVGLLMDIAAKQGKEARSDIHLGICGEHGGDPSSIKFCHKIGLDYVSCSPYRV
                     PIARLAAAHAVLEEE"
     gene            9798..9992
                     /locus_tag="VFD08_02270"
     CDS             9798..9992
                     /locus_tag="VFD08_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09874.1"
                     /translation="MKEIKRALAIQGALMLLISIFAYTQKPKSLSQKQAFVFGTLGLM
                     SLEASHFLKSDITIRRKKGT"
     gene            10018..>10865
                     /locus_tag="VFD08_02275"
     CDS             10018..>10865
                     /locus_tag="VFD08_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK09875.1"
                     /translation="MYKELYPGIFLVEIPLRNNPLKALNCYVIKGEESLIIDTGFDTE
                     ENRSLLFKALEELEIDPKKTRLFLTHLHSDHTGLAAYLGQEGIKVYMGEVDSDFLKES
                     LYEDSFHWVDIQELALAQGLKEDKLDIQKHPGFRFRPKELPPMIKTKEGDCFKVGDYK
                     LEVLDLPGHTPGLQALYDKEKGFLFSGDHLLDSITPNITFWGEEYGDSLGIYLKNLDK
                     LRQMHIRQVFSSHRELPQDHLKRITELIGHHADRLSEIKIILEKLGPSTVRDVTKRMS
                     WDIRAK"
BASE COUNT         3072 a         2545 c         2192 g         3056 t
ORIGIN      
        1 ccttagatta aatactagac ttatagacaa aaaaaagaag acttcttttc tagtcttcaa
       61 ttaaggatta taagagatct ccatgaaagt ttctttctgc gcaggggaac tggtctgtaa
      121 gcagtctttc aaaaaacctc ctggaatttt catagcttcc taagaactct ttaaagagtt
      181 tttctgaagt ttccttggaa taaagctgct caggagccct atcataccaa ttccttctta
      241 cctttaaaaa ggaaaaagaa gaggagctta ttttaagtag ctttacaaat ctgcgatagg
      301 ctagattaat ctcttcagaa ctaagttcaa gatcatagac ctctttggct gccccttgat
      361 aaaaacgagc aatctccttg ggtaactctt ttcctaggtt aatcagggga taaaaggggc
      421 ttctaccacc aggacgcaaa ttccttcttc taagtatggc ttcatccaaa gccatcttga
      481 cgctgtattt atcaaaacct tcgttttgca aagaataaat aaaaaaactg cttccactat
      541 ctagggcata atgggttaaa aaaccataaa gataggcttt tagctctttt ttttctatcc
      601 agtcaaagac atagcaaaga atttcccgtg tcttttcctc gtgaaacatg gtaaaaactt
      661 cctgtccccg gctttgctgg ggtagataat ataaaaaatc tgggcctagg gcgcccaaat
      721 aataggcttt gtcgttctta aatgtaaatt tatgatcctt tgccatatcc ttggcaaaat
      781 aataatgtac ccaatttcct ggcataatgt cctccagtcc ttctataccc caaacttttc
      841 actttaaaaa agacttgcca ggatttaaaa aagcatagag ctctaggctt tatgcttttt
      901 atcctttttt gtaaaaatct agcccaattt tttatttttt cctaggccga taactactct
      961 ttaaagaaac cgtttcatta aaggtcagct tttctggatg agtctcatcc ttggcaaaat
     1021 aaccatggcg aataaattgg taggcctggt ggttgctaga acttcctgca accccttcca
     1081 cctgggcttg atactctttt accgagccct cagccacctg atcggcaagc tctaggctgc
     1141 tatcaaaaac tccttcaatt agggggccaa aacgatagac ccgggcgggc acagatgtct
     1201 ttttatctac ccagtggatg gttccctggg gttttctttc cttaaagcct gatccgcttt
     1261 tggtcttttc atcgtagcta caaactagct caattatttt gccctcttta tcctttatga
     1321 cctcatcaca gcgaataaag taggcaccca ttaggcgaac ttcctttcct ggagaaaggc
     1381 gcttaaagcc cttgggggga ttttcagaaa aatcttctct ttcaatatag atttcacgac
     1441 tcaaagaaat ttccctggag ccaagctggg gattttccac attgttattc atagtcagct
     1501 gttctgattc ttttgtataa ttgctaatca ccactttaag agggtccaaa accgccatca
     1561 ccctaggagt gattatttta aggtcttctc ttataaagtg ctctagcatg gccatctcca
     1621 ccacggagtt ttccttggat accccagtgg cccgaataaa gttcttaata ccctggggcc
     1681 tatagcctct acggcgcatg ccacttatgg taagaagacg aggatcatcc cagccatcaa
     1741 ttaggccctt ttctaccagt tgcataatat gcctttttcc tataatggct ccttcaataa
     1801 tgagtttagc aaactcatac tggcggggct gatagggaac atcggtatgc tcaagatacc
     1861 agtcatagag gggacgattg ttttcaaact caagagagca aagagaatgg gtgatttttt
     1921 ctacataatc ctccatggga tgggcaaagt catacatggg ataaatatgc cagtcatccc
     1981 ctgtacgata atggggctca tcaacaatgc gatagataac aggatcgcgc ataataatat
     2041 ttggatgatc catagaaatc ttggccctta gggtcaggct tccttcagga aactccccct
     2101 ttttcatccg ggtaaaaaga tctctactct cctcaatggg tcgattgcgg tttttactct
     2161 ccaccccacc ccgggtcaag ctgccgcgca tcctacgcat ctcttctggg ctaatatcat
     2221 caatataggc cagcccctta ttaataagaa tatgggctct tttatacatt tcctcaaaat
     2281 aatccgaagc atagcggagc tctttccact caaaacccat ccagcggata tcttctttga
     2341 taagctttac aaaacgctca tcttccttgg ctggattggt atcatcaaag cgcagatagg
     2401 taaagccatt aaattcccgg gcaatatcaa agttgatggt cagggcctta caatggccaa
     2461 tatgaagcag gccattgggc tctggaggaa atcgggtaat ggcatagtca tgcttgccac
     2521 ttttaatatc ctctattaca atatcgcgaa taaagttact tccttctctc atatttcctc
     2581 ctaaaaactg cttgtgtcaa agcccgccag aaaatccttt agataggcta ctgtttcttc
     2641 tactaccttg ggatcaaagg ggctttcaaa attacccagc tccagtatct ttagaaaggg
     2701 taggctaccg ccggcccttg agatggcctt ataatcttcc caaagcttct tctggtcctc
     2761 tctactgcgc ctgagtatct gtagtgccac aatttgggcc agggtatagt ctatatagta
     2821 aaagggcatt tcataaatat gggcttgctt ttgccaaaaa cccccctctt ctagataggg
     2881 ttggccgcca tagtctttcc aaggcatata gattttttct agttcccgcc atttggccct
     2941 tctttgctgg ggcgtcattt gggggttctt atagacctca tgctggaaat gatccaccag
     3001 gaccccatag ggaataaagc ttagggcgcc agctgcatgg gagtagaaaa acttatcact
     3061 atcttcccca aagaaatttt ccatccaggg ccaggtaata aactccatgc ccatagaatg
     3121 aatttctgcg gcctccatac ccggaaaacg ctgggaggga aggggcaggt ccttggctga
     3181 atagacctga aaggcgtggc caaactcatg ggttaggaca ttgacatccc caatggtccc
     3241 attaaaatta gcaaagataa agggcaggcc atagttttca ataaaggtac aatagcctcc
     3301 tggggccttg ccttttttac ttaataaatc catcaactcc ttatctaaaa gggtcttaaa
     3361 aaaagaactg gtctcagcag aaagctcatg atacattttc tccgcctggg aaataataaa
     3421 atcagaatca ccctggggcc tgggcatgcc tcctggatac tcatagccta gatcataata
     3481 atagagtttt tctaggccca gacgcttttt ctgctcctga tagatttctt ctaccagggg
     3541 aactaggaag tttcttactc ccttcctata agcggccacc tcctgctcac cataatcggt
     3601 cctacccatg cggtcataac ctagttgtac aaagtttttg tagcctagtt taagggctat
     3661 agtgtggcga agcttgacca gcctgtcata gatctgatca aagtgctcta gattggcctg
     3721 gaagaagcca aagatggcct cccccgcccc tttacgaagg tcacgatctg gatcttctaa
     3781 aaagacgctc atctcaggca gggtccgctc cttgccctca aaggggattt gtacagaggc
     3841 cattagtttt tggtatttac tagaccactg gctctcctct accagatctt ccataatctc
     3901 ttctttaaag gtcttttttt gcagtagtgc cctatcaata aagtgatcac catagagctc
     3961 tcggtagtcc tctagatagg gactttctag taccgcgcta ataagcccat gcttaaaggc
     4021 agtaaaacgc gccgtatttt catcccaaaa atcattttct ttttcataaa aggagtctag
     4081 ggtatccaca ctgtggcgaa tatgcactaa aaccccttgg ctttctatgt ctttttctat
     4141 ttctatcatt tgcagaaaaa tttcattatg ctccttaaag ctctccgcct cactaaagct
     4201 tgataggagt tcttctgcct tgttttctat ctgatcaagg tctggtcttt gatagactaa
     4261 ttgactaaat ttcatctaat ccccccatat aaggtcttag tactttagga ataataatct
     4321 ttccctctgc tgtttgattg ttttctaata tggctgctag gcatctaccc gtaggaatgc
     4381 cagacccatt tagggtatga agaaagtcca ccttgccctc tttatttcgg tagcgcagca
     4441 tacaacgcct ggcctggaaa tccaggaagt tggaacagga agaaatctcc ctataggtgt
     4501 cataactggg aaaccagacc tctaaatcat aggtcttggc cgaggaaaag cctacatctc
     4561 cagagcatag ctccaccacc cggtagggta gctccaggct ttgaaggatg gcttctacat
     4621 cagagaccat atcttctaat tcatcaaagc tgctttctgg ctctactagt ttaaccagct
     4681 ccacttttcc aaactggtgc tggcggatga ttcccttggt atctctacca gcagaccctg
     4741 cctctctacg atagcactgg gtaaaacttg ttatataaac gggtaggtcc tctaagggaa
     4801 ggatttcttc ccggtgaaaa tttgttaagg taacttcagc agtaggaatc agaaaataat
     4861 catcctctgc gcagtggtac atatcctcaa taaatttagg cagttgtcct gttcctgtca
     4921 tagacaggcg atttgccaca ccgggagaac caatctcggt atacttgcca tctagagtat
     4981 gaaagtcaag aaagtagttc attagggctc tttctaggcg ggcacaggct cccttgataa
     5041 aggtaaagcg gctgcctacc accttggatc ctctttcaaa gtccaaaaaa tttctttcaa
     5101 caccaaggtc ccagtggggg cttgcttcaa attcaaagac ccggggctgg ccccagcggc
     5161 ggatttcttt attgtcctct tctcccttgc ccaggggaat atccttatgg ggcgtattgg
     5221 gcacatagta gagcttttct tcaaagcttt cttctacttg ccttagctct ttatctaggc
     5281 cggaaattct ttctgatagg ccccttagtt cctccaagag ctctgagggg tccttgcctt
     5341 cttttttata ggcaggaatc agtcgagatt tggttttttg ctcatttctt agctgctcta
     5401 cctgaactaa aatctcccgt cttttctttt ctagctccct aatctcaaga aggccaaaat
     5461 caccctgtcc tcgtttttct acgccgcgaa gaatctcatc aaagtccctg cgtattcttt
     5521 taatatcgta cattattcct cctaaaaaaa aactcctccc ttaaaaaagg gacgagaatt
     5581 gttcccgcgt tgccacccta gttggccctt aggcccactt aaagaaaaca tgctccaagt
     5641 ttgattcact ctactccatc tgctgccttg caccaccggc agttctctgt agacttttgc
     5701 aaagctactt tttctcttca acgcatctat taaattagtg taagtgtagc ataaatccgc
     5761 taaaaggtcc agattttcaa gggaaaaaac ctagatgtca ttttcctccc cagcaagaaa
     5821 aagcccctgg ggatccttat ccttaagcaa atgaaaaagc ttcctttgca actcaagcat
     5881 cttcttatga tgggcttttt tctttaactt ggtttttttt cttaattcct tggctgaatg
     5941 gggcttatat ccctctaaac cataaaggcg aaaaagcagg tccgcctcct cagaggaaaa
     6001 tttttccccc agcagcctgg tcaccttgac ccagtctaag gaagttcctt gaggtatttc
     6061 tccagcatcc atcgactctt gtcctccgtc ttctttcctg cactgctgat aatccggtag
     6121 tttccactac cctggccctg gcctaggttt ttttggatta aatccaaagt ttccccatgg
     6181 ctgtcatata ttttgacctg ggggccaaaa aagttttgta tttctttttg taaaaataaa
     6241 aaatgcgtac aaccaagaac cactccactt acctgagcta tttctatgtc ctgggtcatc
     6301 ttttctaatt cactgcgaat tttttctccc ctctactatg ccttcctcta ctagttcaac
     6361 cagggctggc cctggcaggg aaagaatctc tctttggtct tggtagcgct ctagggctaa
     6421 aagataacgg ggactagaca gggtgaagtt tgtagccata accagcagct tgcccctatc
     6481 cccccactcc atagccgaac ttagggctgg ttcaatgcca aagatgggaa tatcaaattc
     6541 cgcctttaga atctcttctg ctgcactggt tgctgtatta caggcaatga ctagggcctc
     6601 acattcaaga tcaataagct cttttgccac ataacgggca aaggagcaga tctcttcttt
     6661 tttcttttcc ccatagggaa cattgagtgc atcgccataa taaatataat cataagtggg
     6721 catttcttgg atcaatttcg ccaagaaact aagcccaccc atacctgaat cgtaaacgcc
     6781 aatttttttc ataataatct cctcaactaa atacatgcct attatagtat attttcctat
     6841 aaatacctag aatctttctc gtttatctga taaaatgagt ccataagagg aggtagaatg
     6901 aaaccctaca ataatgatca agtccttgag cggacagaaa ataaatacat tctctctaaa
     6961 gtcattgcaa aaagagcccg ccagctaaaa gaggaagaag acattgctat tggctatatg
     7021 gccattaaca gagcagtcca gactttgatg gaggatgatt tctcttttaa agtccacccc
     7081 aagaaataga ctgtatcagc agtctatttt tctttaaatt tccttacaag acataacaat
     7141 agtgtataat tagaaggagt atgaatacag aaaggacgtg gcaatatgac aaaatttgtc
     7201 tataccttta acgaaggcaa caaagatatg aaagatctac tagggggaaa gggagcaaat
     7261 ctggcagaaa tgaccaatct tggcctacca gtgcctccag gatttatcat aacaacacaa
     7321 gcctgtaata gcttttatga taaaggccaa atcatttggc cccaattaaa ggaggagatc
     7381 ttctcccatg taaaagagct agaaaaacaa actggcaaat cctatggagg agaaaacccc
     7441 cttcttgtct ctgtaagaag cggaggaaag ttctctatgc ctgggatgat ggatactgtc
     7501 cttaatctag gcatgaatga tgcaaccatt cctattctta ttaaagaatc aggcaatccc
     7561 cgttttgttc ttgatgctta cagacgtttt attcaaatgt ttggcgatgt agtcaaagag
     7621 gttccaaaga aaaagtttga tgaggtcttt gataaggtca aaaaagacaa gggctacagc
     7681 cttgacaccc agctagaaga agaagatctg tcccttattg ttgatgcctt taaagaaatt
     7741 tataaagaaa ataccggtag tgacttcccg caagacccca ttgttcaact cctagaatct
     7801 gtggaagctg tttttagtag ttggaacaat aaccgcgctc gagtctaccg agaagaacat
     7861 aatatttccc ataaactagg cacaggagta acggtccaat ccatggtctt tggaaatatg
     7921 ggagatgact gcggaacagg ggttgccttt actagagatc ctgccacagg agaagccaaa
     7981 ctctttggcg aattccttat caatgcccag ggcgaagatg ttgttgctgg catccgcacc
     8041 cctatgcaga ttgaggaaat gaacctaatt aatgatcatc tctatgatga ctttgtcaaa
     8101 atctgccaca ccttagaaaa tcactataag gatatgcagg atattgaatt taccattgaa
     8161 aaaggcacca tgtacatatt acaaacccgc acgggtaaac gtacggccca ttctgcccta
     8221 cgtattgcag tagatctggt agaagaaggc ctaattgata aaaaccaggc cctacttagg
     8281 gtagagcccg aacaattaga ccaggtcctt cacccaacct ttgataaaaa agccctaaga
     8341 gaagccgttg ttattgccaa gggcctcaat gcctctcctg gtgctgcttc aggacaaatt
     8401 tacttccatg cagccgatgc agtaaaggca gcggaggagg gaatcaagcc tattcttgtt
     8461 agagaagaaa cctccccgga agatcttgag ggaatgatca aggccagtgg gattcttacc
     8521 gctaaaggcg gccgcacctc ccacgcggct gtagttgcaa gaggcatggg caagtgctgt
     8581 gttgccggtt gtgaagccct tgatgtggat gaagacaaaa ggcagttcag ccataaggga
     8641 aagatctata aagaagggga ttttatctcc ctagatggtt ccacaggcta cgtctatgaa
     8701 ggagtcgttg gaacaagtgc ccccatctta gatgagaact ttaaaaccct tatggactgg
     8761 gcagatcatg tagcccatct tacggtccgc gccaatgccg ataccccctt agatgctaag
     8821 caggccctag aatttggagc caggggaatc ggccttaccc gcactgagca tatgtttttc
     8881 caagaacacc ggatctctgc tgttagggag atgatccttg cgcaaaatgc ccaggatagg
     8941 gtaaaagccc ttagaaaaat aatgccctat caaaaggatg actttgttga aatctttaaa
     9001 gttatgaagg gccttcctgt aaccattcgc ctacttgatc cacccctaca tgagtttctg
     9061 ccccatagag atatagaaat aagggccctg gcaaaggaac tagacctatc ctttgaagaa
     9121 ctcaaagccc gagtaactgc ccttcacgag attaatccca tgctgggcca tagaggatgt
     9181 cgactagcca ttagctatcc tgagattgca gccatgcaaa caagggccat tatcctagct
     9241 gctattgagg tgaaaaaaga gggcatccac gttttaccag aaattatggt ccccctagtt
     9301 ggagacaaga aagaactcga atatattgaa gcccttgtac gaccccttgc caaggaactt
     9361 atgcaggaac acgcttgtga gattccctat agttttggaa ccatgatcga ggttccaaga
     9421 ggagccctta ctgcaggaga gattgcaaaa tcagctgagt tttttagctt tggaaccaat
     9481 gatttaaccc aaatggccta tggctttagt cgtgatgatg cagcaggctt tattgatgag
     9541 tacctggaaa aaggcatctt cgatatggac ccctttgtta gcattgaccc taagggagtg
     9601 ggacttctta tggatattgc ggcaaaacag ggaaaagagg cacgtagtga tatccatctt
     9661 ggcatctgcg gagaacatgg aggcgacccc tctagcatta agttctgcca caagataggc
     9721 ctggattatg tttcttgttc accctaccgc gttcccatag cccgcctggc tgctgcccac
     9781 gctgtattgg aggaagaatg aaggaaataa aaagagccct agctatccaa ggagccctta
     9841 tgcttcttat ctcaattttc gcctacactc aaaaacccaa aagtcttagc caaaaacagg
     9901 cctttgtttt tggcacacta ggactaatga gtttggaagc atctcacttt ctcaaatcag
     9961 atatcaccat aagacgtaaa aagggtacct aggtaccctt tcttttagga gaaaaatatg
    10021 tataaagaac tttacccagg aattttccta gttgagatcc cccttcgtaa taatccctta
    10081 aaggccttaa actgctatgt tattaaaggc gaagaaagcc taattatcga cacaggattt
    10141 gatactgaag aaaaccgcag cctgctcttt aaggccctag aagagctaga aattgacccc
    10201 aaaaagacca ggctatttct tacccatctt cactcagacc atacaggact ggctgcctat
    10261 ctaggccagg agggcatcaa ggtctatatg ggagaagttg atagtgactt tcttaaagaa
    10321 agtctttatg aagacagctt ccactgggtc gatatccagg agctggccct ggcccagggt
    10381 ctaaaagaag acaagctgga tatccaaaaa catcccggct ttcgctttcg acccaaggag
    10441 ctccccccta tgatcaagac caaagaaggc gactgcttta aagtgggcga ctacaagtta
    10501 gaggtccttg atcttcctgg ccatacccct gggcttcaag ccctttatga taaggaaaaa
    10561 ggctttttat ttagtggaga ccatctcctt gatagcatta cccccaatat taccttctgg
    10621 ggagaagaat atggggactc tctgggaatc tatctaaaaa acctggataa gctaagacaa
    10681 atgcatatcc ggcaggtttt ttcctcccac cgggaactac cccaggacca tctaaagcgt
    10741 atcactgaac taataggcca tcatgcggac cgacttagtg agataaaaat catcctagaa
    10801 aaacttggcc cctccaccgt aagagatgta acaaaaagaa tgagctggga tataagggct
    10861 aagga
//
LOCUS       DASRNA010000081         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_923517,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000081 DASRNA010000000
VERSION     DASRNA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923517"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VFD08_02280"
     CDS             complement(<1..892)
                     /locus_tag="VFD08_02280"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZK09876.1"
                     /translation="MTTIKLFDKTLKNPVIAASGTFGFGREYQNYMDLSELGGISSKG
                     LTWKKRKGNKGQRIYETASGMMNSIGLQNPSVLGFIKEELPFMLSLDTYILANLGGST
                     IEDYVRGAAELDATAIDAIELNISCPNVKEGGMAFGIRCHDAKAVVEAVRPFTTKPLI
                     VKLSPNAEDIQAMAYTCAQAGADGLSLVNTFNALAIDIENRKPVFDNITAGLSGPCIK
                     PIALRMVRDVALSVDLPIIGLGGISSWQDAIEFIMAGARAIQVGTANFYDPLATKKII
                     EGIKDFMQKEGIKDLEEIRGI"
     gene            complement(879..1598)
                     /locus_tag="VFD08_02285"
     CDS             complement(879..1598)
                     /locus_tag="VFD08_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HZK09877.1"
                     /translation="MEAYRKVTIFENTQLTKDMYEMKVELTDLPQAGQFYMLRAWTDV
                     PYLPRPFSVCDADGKHLSFLYQKVGKGTEIMTKLRPGDQIDLLGPLGKGYPLEKGSIA
                     LIGGGVGIAPLVYLAKHLRGPIDVYLGFKEEAFFTDRFIPYARKVFLSTENGSSGHQG
                     FITELLDDNYDAAYACGNTQMMKSVKDKIDGLLYVSLESHMACGIGACLGCVQESSQG
                     QLCVCKDGPVFNAKEVLFDDN"
     gene            complement(1585..2430)
                     /gene="pyrF"
                     /locus_tag="VFD08_02290"
     CDS             complement(1585..2430)
                     /gene="pyrF"
                     /locus_tag="VFD08_02290"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZK09878.1"
                     /translation="MMDRLFKEVENKSCVCVGLDSHLAYVPPSMKKGTISEVLFNYNK
                     TIIEATRDLVPVYKVQIAYYEALGLQGLIAYRDTLKYLRSLGLLSIGDIKRGDIAATA
                     KEYARAHFEGDFEADFITLSPYMGLDSITPYLPYLERGKGVFVLLRTSNPGSKDLQSL
                     DYKGESLFYHLGDLLKELSKNYIGKEGYSSLSLVVGGSHRQEAEKIRQRYKDQFFLIP
                     GYGAQGGTAKDVRLYLNKGNGGIVNSSRGIITAYKKFEDGEERFSHYTREAVKSMVRD
                     INGSL"
     gene            complement(2427..3617)
                     /locus_tag="VFD08_02295"
     CDS             complement(2427..3617)
                     /locus_tag="VFD08_02295"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZK09879.1"
                     /translation="MLIIKGAQLIDALGEKKGYLLIKDNKIKILDTLKGYEDVPSLDL
                     DGLTLMPAFCDPHAHFRDPGMTHKEDLESGSRAALAGGYTTVNLMANTLPIVDNKKIY
                     EDILERANSLKLIDIHQLMAVTRGFNGQDLIDFEDLPSSLSCLSDDGKGILSNQTMYQ
                     ACLKAKEKDLTIMVHAEDPDLSSEDYRAAEDLITIRDVYLSGHLGAKIHFSHVSTRAS
                     MEAIMEGKKRGFPISCEVTPHHLTLWDHPYKVNPPLRKKEDADFLLHAIKTGFVDAIS
                     TDHAPHTKEEKKEGAPGMIGLESAFSLCYTRLVEENNLPLSSLSRIMSLGGLEVLGVK
                     NRGLLQDGYDAHLVVVDLQKEYTLKEEDLYSKSKNTPFIGKKLRGQVLMTLVGERILY
                     RRKP"
     gene            complement(3618..>3831)
                     /locus_tag="VFD08_02300"
     CDS             complement(3618..>3831)
                     /locus_tag="VFD08_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HZK09880.1"
                     /translation="DITINIIENEVIIEKIKVTLPEHVEGFLKCSNPRCISTSERNII
                     NRFTLVDRATHLYKCDYCDHLYNVEE"
BASE COUNT         1015 a          849 c          808 g         1159 t
ORIGIN      
        1 taatccctct aatttcttcg aggtctttta ttccttcctt ttgcataaag tcttttattc
       61 cttcaatgat ttttttcgta gccaaggggt cgtaaaagtt tgctgtgcca acctgaatgg
      121 ccctagcccc cgccataata aattcaatgg catcttgcca ggaagatatg ccacctaggc
      181 caataatggg caaatctacg cttagggcta catcccggac catacgtagg gcaatgggtt
      241 taatacaagg ccccgaaagg cctgcagtaa tattgtcaaa gacgggtttg cgattttcta
      301 tatcaatagc tagggcgtta aaggtattga ccaggctcaa tccatcagct cctgcctggg
      361 cgcaggtata ggccatggcc tgaatatctt ctgcattagg agaaagctta acaataagag
      421 gctttgtagt aaagggccgg acggcttcta ctacagcctt tgcatcatgg caacgaatgc
      481 caaaggccat gcccccttct tttacattgg gacatgaaat attaagttca atagcgtcaa
      541 tggccgtggc atcaagttca gccgctcccc ttacataatc ctctatggtt gagccaccaa
      601 gattagccag aatataagta tctaggctga gcataaaggg gagttcttcc ttgataaaac
      661 ctagaaccga agggttttga aggccgatgc tattcatcat tcccgaagcg gtttcatata
      721 ttctttgtcc cttatttccc tttctttttt tccaagtaag gcccttgctt gagataccac
      781 caagctcaga aagatccata tagttttggt attctctacc aaagccaaag gttcctgatg
      841 cagcaataac agggtttttc agtgtcttat caaaaagttt aattgtcgtc aaagagcacc
      901 tcctttgcat tgaatacagg accgtctttg cagacacata gctggccttg ggagctttct
      961 tgaacgcatc ctagacaggc gccaatgcca caggccatat gagattccag ggacacataa
     1021 aggagtccat caattttgtc tttaacagat ttcatcatct gtgtatttcc acaggcatag
     1081 gccgcatcgt agttatcatc aagaagctct gtgataaatc cctggtgacc cgaagaacca
     1141 ttttctgttg ataggaaaac cttcctggcg tagggaataa atcgatcggt aaaaaaggct
     1201 tcttccttaa aacccagata aacatctatg gggcccctaa ggtgtttagc aagatagacc
     1261 aggggggcaa ttcccactcc accgccaata agggcaatgc ttcctttttc taagggatag
     1321 cccttgccta gggggcctag aagatcgatt tgatctcctg ggcgcagttt tgtcataatt
     1381 tctgttccct ttcccacctt ttggtagagg aaactaaggt gtttaccatc cgcatcacaa
     1441 acagaaaagg ggcggggaag ataggggaca tcagtccaag cccgtagcat ataaaattgt
     1501 cctgcttggg gcaaatctgt taattctacc ttcatctcat acatatcttt agttagctgg
     1561 gtattttcaa agattgtcac ttttctatag gcttccatta atatccctca ccatgctctt
     1621 aacagcttct ctagtgtagt ggctaaatct ttcttcccca tcctcaaatt ttttataggc
     1681 agtaataatt cctctggagg aatttacgat gcccccattg cccttattaa gatagaggcg
     1741 aacatctttt gctgtgccac cctgggctcc atagccggga ataagaaaaa actggtcctt
     1801 gtatcgttga cggatttttt ctgcttcctg tctatggcta cccccaacaa caagactaag
     1861 actactgtag ccttcttttc ctatatagtt tttgctcagc tcttttagaa gatcgcccag
     1921 atgatagaag aggctttctc ctttatagtc aagactttgt aaatctttgc ttccaggatt
     1981 actggtacgc aagaggacaa aaactccctt gcccctttca agatagggta gatagggggt
     2041 aatgctatct aggcccatat aggggcttag ggtaataaag tctgcttcaa aatctccctc
     2101 aaaatgcgcc cgggcatact cttttgccgt ggcagctata tcgccccttt ttatatcgcc
     2161 aatactaagt aggcctagac ttctaaggta tttgagggtg tccctatagg cgattaggcc
     2221 ttgaaggccc agggcctcat aataggcgat ttgaacttta tagactggaa caagatccct
     2281 tgtagcttcg ataattgttt tgttatagtt aaataagact tcggatattg tgcctttttt
     2341 catggaaggg ggcacataag ctaaatggct atccaggcca acacatacac aactcttatt
     2401 ttctacttct ttaaaaagtc ggtccatcat ggctttctcc tgtaaagaat tctttctccc
     2461 accaaggtca ttaggacttg gcctcgaagt tttttcccaa taaaaggggt gtttttagat
     2521 ttggagtaaa ggtcttcttc ttttagtgta tattcttttt gcaaatcaac cacgacaaga
     2581 tgggcgtcat agccatcttg tagcaggcct ctgtttttaa cccctagaac ttctaggcca
     2641 ccaagactca taatccggct tagtgaactt aggggtagat tgttttcttc tacaagccta
     2701 gtgtagcaga ggctaaaagc gctctcaagg ccaatcattc ccggggctcc ctcttttttt
     2761 tcttccttgg tgtggggagc gtggtcggtg ctgatagcat caacaaagcc tgttttaatg
     2821 gcatgaagaa gaaagtcggc atcttctttt ttgcgaaggg gtggattaac cttgtaggga
     2881 tgatcccaga gggtcaaatg atggggggtc acctcgcagc tgataggaaa acctcttttt
     2941 tttccttcca taatagcctc catggaggcc ctagtggaaa catgagaaaa atggatttta
     3001 gcacccagat ggccgctaag ataaacatct cttatggtaa tcagatcttc agctgccctg
     3061 taatcttcag aagaaagatc aggatcttca gcgtggacca taatggttag atccttttct
     3121 tttgccttaa gacaggcctg gtacattgtt tggtttgaga ggatgccttt gccatcatcg
     3181 cttaggcagc taagagagga gggcaagtcc tcaaaatcaa tcaggtcctg cccattaaag
     3241 cccctagtga cggccataag ctgatggata tcaattagct taagggaatt tgccctttct
     3301 aagatatcct catatatttt tttattatca acaattggta gggtgttggc cataagatta
     3361 acggtagtat agccaccagc tagagcggcg cggctaccac tttctaaatc ttctttatgg
     3421 gtcattcctg gatctctaaa atgggcatga ggatcacaaa aggcgggcat aagggttaga
     3481 ccatcaagat ctagggaggg cacatcctca taacccttga gggtatccag gatctttatt
     3541 ttattgtctt taataagaag atatcccttt ttttctccaa gggcatcgat tagttgggcc
     3601 cctttgataa ttagcattta ttcctccaca ttgtataaat ggtcacaata atcacactta
     3661 tatagatggg tagcgcggtc tactagagta aacctgttta ttatattgcg ctctgaagtg
     3721 gaaatgcagc ggggattact acatttgaga aagccttcta catgttcagg tagtgttacc
     3781 ttaatttttt caataatcac ttcattttct atgatattta tggttatatc c
//
LOCUS       DASRNA010000082         3873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_923549,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000082 DASRNA010000000
VERSION     DASRNA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923549"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(427..1647)
                     /gene="pepT"
                     /locus_tag="VFD08_02305"
     CDS             complement(427..1647)
                     /gene="pepT"
                     /locus_tag="VFD08_02305"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005545156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HZK09881.1"
                     /translation="MKIHERLINYVKVDTTSDETSTTCPTTPGQMELANMLKDELEEL
                     GLRTTLSDDGYLFGHLQANSQKDVYHLGFLAHIDTAPDAPGANVSPRIQLYEGGEIEL
                     ESGLILSPKDFPNLNNYIGQELMLTDGRTLLGADDKAGIAAIMEVLKRLKEDPDILHG
                     PISVAFTPDEEIGRGPHRFDVEGFGAEYAYTIDGGPVGELQFENFNAAGAEITFIGRS
                     VHPGTAKDQMISALHMAMDFEKALPELEKPMYTQGYEGFTHLNNLSGNVGKAVAQYII
                     RDHDKAKFLEKKARVEEIVASMQKKYGEDMILLTMEDSYYNMREKIEPVMFMIDYAKE
                     AMNNLGITPIIKPVRGGTDGAQLSYKGLPCPNLFTGGENFHGPYEFISLDALEKSADL
                     ILEVIQLYEKKGKH"
     gene            complement(1726..3039)
                     /locus_tag="VFD08_02310"
     CDS             complement(1726..3039)
                     /locus_tag="VFD08_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK09882.1"
                     /translation="MTFYCNEPKAIKIYDLLHIFFDEIEWGSEKSDLVYKDGLFLFEE
                     KKIKLKTTEKYILEIYDALEAHYQPISSWGILSGVRPLKIVHQEKKKGKSDKEIFNKL
                     KNSYRLSDNKARLLIEIASRQNKIYQGDRDKLALYISIPFCPSICSYCCFHTRSYEKR
                     WAQKYLSNLHQDISYAKKRIKEQGKIVDCIYIGGGTPWVLEEDQLEELFGALSGFTKV
                     KEITFEGGRTDVLTPKKAGLVSSFATRVCLNPQSLTRGITPLQGRPEADDMKKWVEYF
                     KKEGLFVSSDLIAGLPGESLDEFLFSLEELIGLNPENITLHNLSLKKGAQLKKVAASA
                     SVGLMLEEGYTRLEKASYRPYYIYRQKNMVDRGENVGFEKENTASIYNIRMMEDAHEI
                     LSIGSNAVSKKIRNKQALRRATPKDINLYMKEKEIRKTIIDDLFL"
     gene            complement(3036..3656)
                     /locus_tag="VFD08_02315"
     CDS             complement(3036..3656)
                     /locus_tag="VFD08_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK09883.1"
                     /translation="MIKRLMIPAMGQNCYVYYDEKTKNGFLVDPGMVDKRISDFLEKE
                     DIKLEAILLTHGHGDHIMGVEHYKEQHNCPIYAHKDEREVLENHHHNHSHLIGGTVVE
                     LMDVIYFEDGDILELAGTKIKCIHTPGHTLGGVSYLTDEGLFSGDTLFKLGIGRYDLY
                     GGDLKVLEHSIVEILYELPEETKVYPGHGVPTTIGYEKKNNPHFRK"
     gene            complement(3656..>3873)
                     /locus_tag="VFD08_02320"
     CDS             complement(3656..>3873)
                     /locus_tag="VFD08_02320"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZK09884.1"
                     /translation="FTLYGDSRKGNRPSFVRSAKHEKAEKMYLQMKDELEKLGHKVQT
                     GRFAASMDVELVNDGPVTILLDSRKEF"
BASE COUNT         1089 a          855 c          639 g         1290 t
ORIGIN      
        1 catagagctc aattagcttt gctatctctt ctttactcct gcgctttttg ataatcctat
       61 cttccattat taccacctcc tcacagagat aataataagc taaaagggga ttagttaaaa
      121 ggcgcccttg gcttgacgct taccaccctt acacataatt ctcgtacttg tctatagagt
      181 cgatttctct aaaccaacat catttgacga agaccctgta aaaacagata tctaatcctt
      241 tgcagacaag taataccgat tagtgacgac acctttaaat ataaaaaggc gagctaattc
      301 acactggatt agcccaccct tacatataaa tctcaaactc gtctatagag tcgatttctc
      361 taaaccaaca tcatttgacg aagaccccct ttttctagag gatcccacag ttttgagcaa
      421 ttctagctag tgctttccct ttttttcata gagttggatc acttccaaaa taagatcagc
      481 gcttttttcc aaggcatcta gagaaataaa ctcatagggc ccatggaagt tttctccccc
      541 tgtaaataag ttaggacaag gtagaccctt ataggaaagt tgagctccat cggttccacc
      601 cctaacaggc ttaataatgg gcgtgatacc aagattattc atggcttctt tcgcataatc
      661 tatcataaac attaccggtt caattttttc tctcatatta taatagctat cttccattgt
      721 aagaagaatc atatcttctc catacttttt ttgcatggaa gcaactattt cttctactct
      781 tgcttttttc tcaaggaact tagccttatc atggtctctt ataatatact gggctacagc
      841 tttaccgaca ttgccactaa ggttgtttag atgggtaaac ccttcatagc cctgggtata
      901 cataggtttt tctagttctg gtaaggcttt ttcaaaatcc attgccatat gaagagcgct
      961 aatcatttga tcctttgccg tacccggatg aacgcttcga ccaataaagg taatctcagc
     1021 ccctgctgca ttaaagtttt caaactgcaa ctcacctact gggcctccat caatggtata
     1081 ggcatattct gctccaaagc cttctacatc aaatctatgg gggccccttc caatttcctc
     1141 atctggggta aaggcaacag agataggacc atgaagaata tcaggatctt cttttaatct
     1201 tttaagaact tccattattg cagcaattcc tgccttatca tcagctccta aaagagtacg
     1261 tccatcggtt agcattagtt cttgcccaat ataattattt aaattaggaa aatctttcgg
     1321 tgataagata aggccactct caagctcaat ctctccgcct tcatagagtt gaatccgagg
     1381 acttacattg gccccagggg catcaggtgc cgtatcgata tgggctagaa aacccagatg
     1441 atatacatcc ttttggctat tggcctgaag atgaccaaat aaatatccat catcagaaag
     1501 agtggttcta agacccagct cctctagttc atccttaagc atattagcaa gttccatctg
     1561 tccaggagtt gtagggcaag tagtagaagt ttcatcactg gtcgtatcaa cttttacata
     1621 atttatcaaa cgctcatgta ttttcattat tgtcctctct ttttgatgag accttggctc
     1681 atctaagctc tagtgcaatt gtaataacta taccctcatt taattttata aaaacaaatc
     1741 atctataata gtttttctta tttctttttc cttcatatat aagttgatat ctttaggggt
     1801 cgccctgcga agggcctgct tatttcttat ttttttgctt actgcattag atccaataga
     1861 gagaatctca tgagcatctt ccatcatgcg gatattgtaa atactagcag tattttcttt
     1921 ttcaaagcct acattctcac cccggtccac catgtttttt tggcgataga tataataagg
     1981 cctatagctt gctttttcta ggcgtgtata gccttcttct aacataagac ccaccgaagc
     2041 agaagctgca actttcttta actgcgctcc tttttttaag gaaagattat gcagggtgat
     2101 gttttcagga tttagtccta ttagctcctc taaggaaaac aaaaactcat ccaaactttc
     2161 tccaggaagg cccgcaataa ggtctgagga tacaaaaagt ccctcttttt taaagtattc
     2221 cacccatttt ttcatatcat ctgcttctgg tctaccctgt agaggagtaa tacctctagt
     2281 aaggctttgg gggtttaagc agaccctagt tgcaaaagaa gagacaagac ctgccttctt
     2341 aggggttagg acatcggtcc tgcccccttc aaaggtaatt tccttcacct tagtgaagcc
     2401 acttaaggcg ccaaatagtt cttctagctg gtcttcttca agaacccagg gagttccccc
     2461 accaatataa atacaatcaa caatttttcc ctgctctttg atgcgttttt ttgcatagga
     2521 aatatcttga tgaagattgc tcagatactt ttgcgcccat cttttttcat agcttcttgt
     2581 gtgaaaacaa caataggaac aaatgctagg acaaaaaggg atagatatat atagtgccag
     2641 tttgtcccta tcaccttgat aaatcttgtt ctgccggctt gctatttcta ttaaaagtct
     2701 ggccttatta tcagaaagtc tataggagtt tttaagcttg ttaaaaattt ccttatcact
     2761 ttttcccttc tttttttcct ggtgaacaat tttaagggga cgtaccccgc ttaaaattcc
     2821 ccaagaagaa ataggttgat agtgggcctc aagagcgtcg taaatctcta agatatattt
     2881 ttctgtagtc tttagcttaa tttttttttc ctcaaaaaga aaaaggccat ctttataaac
     2941 aagatcgctt ttttcgctgc cccactctat ttcatcaaaa aatatatgca aaagatcata
     3001 gatttttatt gcctttggtt cattgcaata aaaggtcatt ttctaaaatg ggggttattc
     3061 tttttttcat agccaatggt tgttggaact ccatggccgg gataaacctt ggtttcttct
     3121 ggaagttcat acaagatttc aacaattgag tgctctaaga cttttaagtc acccccatag
     3181 aggtcatatc gtccaatacc cagtttaaaa agggtgtctc cactaaagag gccttcatct
     3241 gtaagatagc ttactccccc tagagtatgg cctggcgtgt gaatacattt gatctttgtt
     3301 ccagcaagct caagaatatc accatcttca aaatagataa catccattaa ctccaccaca
     3361 gtccccccga taagatggga gtgattatga tgatggtttt caagaacttc cctttcatct
     3421 ttatgggcat aaatgggaca attgtgttgc tctttatagt gctctacacc cattatatgg
     3481 tccccatggc catgggttaa aagtatggcc tcaagcttaa tatcttcttt ttctaaaaaa
     3541 tcacttattc ttttgtccac catgcctgga tcaacaagaa agccattttt tgttttttca
     3601 tcataatata catagcagtt ttgccccata gcaggtatca taagtctttt tatcatcaaa
     3661 attcctttct actatctaaa agtattgtca caggtccatc atttaccagt tcaacatcca
     3721 ttgaagctgc aaaacgccct gtttgaacct tatgtcctaa tttttctaat tcatctttca
     3781 tttgaagata cattttttcg gctttttcgt gtttagcact tcgcacaaag gaaggtcgat
     3841 ttccctttct actatcgcca tagagggtaa att
//
LOCUS       DASRNA010000083         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_843787,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000083 DASRNA010000000
VERSION     DASRNA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843787"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFD08_02325"
     CDS             complement(<1..683)
                     /locus_tag="VFD08_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HZK09885.1"
                     /translation="MLNKKKFKKLALEYGADAVGFTTAEELSVRPLYRPALAFEEKSL
                     EKKIKPRAHFPQARGILVILKGYKLYENRLDKGKIKVASSSPRDYHLEVGEILEALIL
                     ELEKDGIKGLAICDKEGLMDKEIAKRAGLGFIGKNTLLIHPDLGSAHNIGYILMDAEL
                     VPDKEMAKDCGKCKACILACPQGAIGDYQLEAKKCLSYITQWKEAEYTPLKGCFYGCD
                     ICLRACPYN"
     gene            complement(726..2348)
                     /locus_tag="VFD08_02330"
     CDS             complement(726..2348)
                     /locus_tag="VFD08_02330"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZK09886.1"
                     /translation="MSEKIKERLLNSVIFVLFSLLFVTLSRAWLIRDLFAANLGDLSF
                     NLYIGVKLATMVCVSGLFIINLYSFSLFKGLRQLLFSLIFLVLLIIVALSLVVSVNDS
                     LPVTLLNRPQLQGVLVFLILVIMGLLFLKLVLIRKTIQLDLPIRRIPFFLIGFVVLII
                     VAYIWFIKEPRLIKEMEVALIFILLFSNIMGYLVNYLVDGSRITGNVLFLLIVASVSF
                     FFKQGDTLSTGYEGVLEGAFNLVALVFLLNAFYQELFTSYLLEVEELNYQRDLYTENL
                     KEVVEERTLELQKANDQLESEIDAARRLQQSLLPMKDIVYSKASFVSENFPCERLSGD
                     FYDIYSIDEDKVGMYILDVSGHGINAALMNIYIYNYIRTNSPLIKRLRGDQPHENLRY
                     LYDEFNKMNFPDHMHVVIFIASYDMKTGVLSYASGGLNVSPILVRESGEIKLLDDSSG
                     FPICKMADYFTPEYASAQIQLFGGDRVFFYTDGLLDERQGLNLSMDDLKILLLESLDQ
                     PLVAVDRALEKRIDPVKNNLVDDISYFILEAK"
     gene            complement(2360..>3154)
                     /locus_tag="VFD08_02335"
     CDS             complement(2360..>3154)
                     /locus_tag="VFD08_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HZK09887.1"
                     /translation="VVLIAGPSSSGKTTFTHRLKIQLMAEGVFSQTVSLDNYFVNREL
                     TPLDENGNYDFECIEALELDLLNRNIEDLLEGKEIGLPIFDFKLGKRQDEAYKIQLKK
                     NEIIIFEGIHGLNEKLTSGVLQKDKFKIYISALTSLNLDQHNRIPTTDNRILRRMVRD
                     HFTRGHGAEDTLAMWDSVRRGEERHIFPFQEEADSVFNSALVYELAVIKKYALPLLEE
                     IGLDSPVYSQAKRLKKFLYYLKSIEDDRIIPSTSLLREFIGGSSIL"
BASE COUNT         1010 a          671 c          547 g          926 t
ORIGIN      
        1 ctattatagg ggcaggctct caaacaaata tcacagccat aaaaacatcc cttaaggggt
       61 gtatattcgg cctctttcca ctgggtaata taagaaagac attttttagc ttctagctgg
      121 tagtccccaa tggccccttg cgggcaggcc agaatgcacg ccttacactt gccacagtct
      181 tttgccattt ccttatctgg cacaagctct gcgtccatta gaatataccc aatattatgg
      241 gctgagccca aatcgggatg aataagcagg gtgtttttgc ctataaatcc aagtcccgct
      301 cgcttggcaa tttctttatc cataaggcct tccttatcac aaatagctag gccctttatt
      361 ccatcttttt ctagctctaa aataagagct tcaagtattt ctcccacctc aagatgataa
      421 tctcttggag aggaacttgc aacttttatt tttcctttat caagtcggtt ttcatagagc
      481 ttgtagcctt tgagtataac taagattccc ctagcctggg ggaaatgagc ccttggcttg
      541 attttctttt ctaaagattt ttcttcaaag gccaaggctg gtctatacag aggccttaca
      601 ctaagctctt ctgctgtcgt aaaaccaaca gcatcagcgc catattctag ggcgagtttt
      661 ttaaactttt tcttgttcaa catatactat tactatacca cagccccctt gctaaaaaaa
      721 gatatctact tggcttccaa gataaaataa ctgatatcat cgactaaatt atttttcaca
      781 ggatctattc gtttttcaag agctcggtcc actgccacca ggggttgatc taggctttct
      841 aacagaagga tttttaaatc atccatagac aaatttaagc cttgtcgttc atctaagagg
      901 ccatcagtgt aaaagaaaac cctatcccca ccaaagagct ggatctgagc agaagcatac
      961 tctggggtaa agtaatctgc catcttgcaa atgggaaagc ccgatgaatc atcaagcaac
     1021 ttaatctcgc cactttcccg aacaagaata ggtgaaacat ttaggcctcc actggcataa
     1081 cttaaaaccc ctgttttcat atcataagaa gcaataaaga ttaccacatg catatggtca
     1141 ggaaagttca ttttgttgaa ttcatcatat aaatatcgta agttttcatg gggctggtcc
     1201 cctcgaagtc gttttatcag gggagaatta gtccgaatat aattataaat ataaatattc
     1261 ataagggcgg catttatacc atgtcctgag acatctagga tatacatacc caccttgtcc
     1321 tcatcaatac tataaatgtc atagaagtcg ccactaagac gctcacaggg aaagttttct
     1381 gaaacaaaag atgcctttga atagacgata tccttcatag gtagaagaga ttgctgcaat
     1441 cttctggctg catcaatttc actctccagc tgatcatttg ctttttggag ttctaaggtc
     1501 ctttcttcta ccacctcttt aaggttttct gtatataaat ctctttgata attcagctcc
     1561 tctacctcca aaagatagga ggtaaaaagc tcttgataaa aagcattcag caaaaagaca
     1621 agggccacta gattaaaggc tccctctagc accccttcat aacccgtaga aagagtgtct
     1681 ccttgcttaa aaaagaaact aacagaggca acgataagta aaaagagcac attgcccgtg
     1741 attctagatc catcgacaag ataattcacc aaatagccca ttatattgct aaataacaaa
     1801 ataaagataa gggcaacttc catttcctta atcaaacgag gctctttaat aaaccaaata
     1861 taggcaacaa ttataagcac aacaaaacca ataagaaaaa agggaattct tcttattggt
     1921 agatcaagtt gaatcgtttt acgtataaga acaagcttta aaaataagag acccattata
     1981 accaggataa gaaaaacaag tactccttgt aattgcggcc gattaagtag ggttacaggt
     2041 agtgaatcat ttaccgaaac cactagagaa agggcaacaa tgataagaag aaccaaaaaa
     2101 atcaaactaa agagcagttg tcttaagccc ttaaaaaggg aaaagctata gaggttgata
     2161 ataaaaagac cgctcacaca aaccattgtg gccaatttta caccaatata tagattaaaa
     2221 gacaagtctc ctaagttcgc tgcaaaaagg tctctgatta gccaagcgcg gctcagtgta
     2281 acaaaaagca gtgaaaaaag tacgaagatc acactattta ataatcgttc ctttattttc
     2341 tcactcatga actctcctct tagaggatac tacttcctcc gataaactct cttaatagcg
     2401 atgtactagg gataattcta tcatcttcaa tactctttag atagtacaga aatttcttta
     2461 atcgttttgc ctgtgaatag acaggactat ctagtcctat ttcttcaaga agaggaaggg
     2521 catacttctt aattaccgca agctcatata ctaaggcaga gttaaaaaca gaatctgcct
     2581 cttcttggaa gggaaaaata tgtctttctt ctccccggcg cactgagtcc cacatagcta
     2641 aggtgtcttc cgccccatgg cccctagtaa aatgatccct taccatacgg cgcaatattc
     2701 tattatctgt ggttggaatg cgattgtgtt ggtctaaatt taggctggta agtgcagaaa
     2761 tataaatttt gaatttgtct ttttgtaaaa cgcctgaagt aagtttttca tttaaaccat
     2821 ggatcccttc aaaaataata atttcgttct tttttaattg tattttatag gcctcgtctt
     2881 ggcgctttcc cagcttaaaa tcgaatatgg gaaggccaat ttctttgcct tcaagcaagt
     2941 cttcaatatt tctattaaga agatccagtt ccagggcttc tatacactca aagtcatagt
     3001 taccattttc atccaagggg gtcaattctc tattaacaaa ataattatct aaagaaacag
     3061 tctgtgagaa gactccctca gccatcaact ggatttttaa acgatgggta aaggtggttt
     3121 tcccagagga gcttggtcct gcaataagca caac
//
LOCUS       DASRNA010000084         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_925010,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000084 DASRNA010000000
VERSION     DASRNA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925010"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFD08_02340"
     CDS             complement(<1..629)
                     /locus_tag="VFD08_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK09888.1"
                     /translation="MAVIETKNLTKYYGKALGIKDVSLSVEEGEIFGFIGPNGAGKST
                     TIRLLMSLIYPSSGEAKLFGKDAIKYGPELRRNIGYIPSEIFYYDKMKVIDLLHYSAS
                     FYEKDCSKKIKELSDTMELDLNRKIEDLSYGNKKKVGIVQGLLHEPNLIILDEPTGGL
                     DPLMQQKFFHLIREENKKGATVFFSSHILGEVQELCNRVAIIRKGSIIEV"
     gene            complement(783..989)
                     /locus_tag="VFD08_02345"
     CDS             complement(783..989)
                     /locus_tag="VFD08_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09889.1"
                     /translation="MNGYALLGILAFLYAGMVFFITYKKPPKIWNISKIQLFEKVLGE
                     KGTVIFFYAFGLLALALGIWLMTK"
     gene            complement(1021..2022)
                     /locus_tag="VFD08_02350"
     CDS             complement(1021..2022)
                     /locus_tag="VFD08_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK09890.1"
                     /translation="MFSRIWNIFKRDAKVNSKDFISLYILVVPILFAILINIFSPGIN
                     DTTINLALIEGENPAQVEYFQQFAKVELFKDLDQITKRVEKRDSIIGIIPENNNYYLL
                     TQGNESQGIVDYAKLLHSFYELDINIEDSNSELIEFGRTVPPLKKTLVNTSILLISIL
                     GGMLIALNIVEEKVDKTLSAINLTPTPRRVFLLGKSVIGVLFALVGSLALVFITGFGG
                     VNILQILLVLLVTSLLSILVGFIQGLTNKDIINAAASIKLLFLPLIAGVLAIELLGDK
                     WQKLFYWNPFYWAYKGNDIILAESGTWGEVLLYTGIVLVISGVVYLFLAPKIRKGLE"
     gene            complement(2035..3144)
                     /locus_tag="VFD08_02355"
     CDS             complement(2035..3144)
                     /locus_tag="VFD08_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK09891.1"
                     /translation="MKLFSSFIREAKIASRGFYFYVEIFMALLILALLLFVVPDNFNT
                     KTSEYLYLDMPQEASEAYLAFILDNTVDGKAEQVKVKIDKKEVDASLYTTEDSKIYIL
                     NSQEEVAYIADKEREIAATIKMDENHSLTYEYYLQGYESDRYKDLLKILHVESTDVIE
                     EKMDKQDVRALSTNHTFLSDRENMLPSVLVFNGSLMGFFLIAAYIFLDRQEGVIKAFA
                     LTPSPIWQYLLSKIGVLLLVCVSSSFIVIVPIMKTRPDYLLLLVLLVTSSFFTSSLGL
                     LIASFYDDIMQAFGILYVFMILMIIPNVAYFMPSWNPSWVKIIPTYPMLEGFRSIILG
                     NGDTIYILTISAGFLLAGMVLFALSNYRYKKTLVG"
     gene            complement(3145..>3967)
                     /locus_tag="VFD08_02360"
     CDS             complement(3145..>3967)
                     /locus_tag="VFD08_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK09892.1"
                     /translation="NKDDIYQVKDVSFEIEKGEVFGFLGPSGAGKSTVQNILTGLLQL
                     QEGEATVGGYDIRKMSKDMFNKIGMSFEQSNVYSKLTAKENLDFYRKLFDVPTRDPME
                     LLKLVGLDGKENTRAGEFSKGMKHRLTFARSMINNPEIWFLDEPTTGLDPSIAADIKD
                     IIKEKNSQGVTIFLTTHNMFIADELCDRVAFIVDGEIKLIDSPESLKLKYGDQLVDIK
                     YVENKELVEKTLSLVVQEDKKALQDIIETKEVQTIHTKEATLEEIFIKVTGRELV"
BASE COUNT         1176 a          742 c          696 g         1353 t
ORIGIN      
        1 acttcaataa tagaaccttt tcttataatg gccaccctat tacaaagttc ctgaacctcc
       61 ccaaggatat gagaagaaaa gaagactgta gctccttttt tattttcttc tcttattaag
      121 tgaaaaaact tttgttgcat aagaggatcc agtccacctg ttggctcatc caggataata
      181 agattaggct catgaagaag tccctgcacg atgcctactt ttttcttatt cccataggat
      241 aggtcttcga tttttcgatt tagatccagc tccatagtat cgctgagttc tttgattttt
      301 ttgctacaat ccttctcata aaagcttgca gagtagtgca agagatctat gactttcatt
      361 ttgtcataat aaaaaatttc ggaagggata tatccgatat tccttctaag ttcagggccg
      421 tattttatgg cgtcttttcc aaacagtttt gcttcccctg aggagggata gattagactc
      481 ataagcagtc ttatggttgt tgattttccg gctccattgg ggccgataaa accaaaaatc
      541 tctccttctt ctactgatag acttacatct tttattccca aggccttacc atagtatttt
      601 gttaaatttt ttgtctctat tacagccata ttttttctcc ttattggatg agctaggtag
      661 gaagtgttaa aaagctctct agctatagta taaagtagga ctgatctttt agcctttctt
      721 atttaaagga aaacagccaa gataccgtct ttatcaaggc tgttttagag taagaaaact
      781 ctttacttag tcattagcca tatacctagc gctagggcta atagtccaaa ggcgtagaaa
      841 aatataacag ttcctttttc cccaagaact ttttcaaaaa gctgtatttt agaaatattc
      901 catatttttg gtggcttttt gtaggtgata aaaaagacca ttccagcata aagaaaagct
      961 aagatcccaa gaagtgcata tccattcata tttattcctc ctatgtaagg tcttataagt
     1021 ctattccaat ccttttctta tttttggggc taagaaaagg taaaccacac cacttataac
     1081 cagaacgatg ccggtgtaga gtaatacttc tccccaagtt ccactctcag ctaagatgat
     1141 atcattgcct ttataagccc agtaaaaagg attccagtaa aagagttttt gccatttatc
     1201 gcctaagagt tcaatggcta agacccctgc aattaggggt aaaaagagaa gtttgatact
     1261 ggcggctgca tttataatat ccttatttgt tagaccttgt ataaagccaa ctagtatact
     1321 cagcaggcta gttaccaaaa gaacaagtag tatttgcagt atattgactc caccaaagcc
     1381 tgtgataaag accagggcta gggatccaac tagggcaaaa aggactccga ttacagactt
     1441 gcctagtaga aaaactcttc tgggtgtggg ggtcaagttt atagcactaa gggtcttatc
     1501 taccttttct tctactatat ttagggctat tagcattcct cctagtatag atataagtag
     1561 gattgaagta ttaaccaggg tttttttaag aggaggaacc gtccgtccaa attcaataag
     1621 ctcactattt gagtcttcta tattgatatc taattcataa aagctatgta agagctttgc
     1681 ataatcgacg attccttggc tttcatttcc ctgggttagt aagtaatagt tgttgttttc
     1741 tggaatgata ccgataatgg agtctctttt ttccactctt tttgttattt gatctagatc
     1801 cttaaagagt tccaccttag caaattgctg gaaatactct acttgtgctg gattttcacc
     1861 ttctattaga gcaaggttaa ttgttgtgtc gtttattcct ggtgaaaata tattgattaa
     1921 aatcgcaaag agtattggaa ctacaagtat atataaggag atgaaatctt ttgaattgac
     1981 ttttgcatct cttttgaaaa tattccatat tctactaaac ataaccaacc ttccttatcc
     2041 tactagtgtt ttcttgtatc tgtagtttga aagtgcaaat aaaaccatcc ctgcaagtag
     2101 aaagccggca gatatggtta aaatatatat ggtatcgcca tttccgagaa taatcgatct
     2161 aaagccttct aacataggat aggtagggat gatttttacc cagctaggat tccaacttgg
     2221 cataaagtaa gcgacattgg gaataatcat tagtatcata aaaacataga ggataccaaa
     2281 ggcctgcata atatcatcat aaaaacttgc tattaaaagt cctagtgatg aggtgaaaaa
     2341 ggagctagta acaagtaaaa ctaggagtag gagataatct ggtctagttt tcataatagg
     2401 aacaatcacg ataaaactag aggatacaca aacaagcaat agaactccta ttttactgag
     2461 taaatactgc cagatgggag agggagtaag agcaaaggcc tttatgactc cttcttgtcg
     2521 atctaaaaat atataggcag caataagaaa aaatcccata agagaaccat taaaaacgag
     2581 gaccgaaggt agcatgtttt ctctatctga aagaaaagtg tgatttgttg atagggccct
     2641 aacatcctgc ttgtccattt tttcttctat tacatcggtt gattccacat gaagtatctt
     2701 taatagatct ttatagcgat cggattcata cccttgcagg tagtattcat aagtaagact
     2761 atgattttca tccattttga ttgtggctgc gatttctctt tctttatcag ctatataggc
     2821 gacttcttct tggctattta gaatgtaaat tttactatct tctgttgtgt atagggaggc
     2881 gtctacttct tttttgtcta ttttcacctt tacctgttcg gctttcccat cgacggtatt
     2941 atctagtata aaagctagat aggcttcact agcctcctgg ggcatatcca agtataggta
     3001 ctcagatgtt tttgtattaa aattatcggg aacaacaaag agcagtaatg ctagtatcag
     3061 tagggccatg aaaatttcaa cataaaagta aaagccccta gatgctattt ttgcttccct
     3121 aataaagcta gaaaagagtt tcatttaaac aagctccctt cctgtcactt taataaatat
     3181 ttcttcaaga gtagcttctt ttgtatgtat tgtttgaacc tcttttgttt caattatatc
     3241 ttgaagtgct tttttatctt cttgaaccac taaggaaagg gttttttcta ctagctcttt
     3301 attttctaca tactttatat ctactagctg gtctccgtat ttaagtttta agctttcagg
     3361 agagtctatg agttttattt ctccatcaac gataaaggcc accctatcac aaagctcgtc
     3421 tgcaataaac atattgtggg tcgttaaaaa tatggttaca ccttggctgt ttttttcttt
     3481 tataatatct ttaatatcag cagcaatgga gggatcaagc cctgtggtgg gctcatctaa
     3541 aaaccaaatt tccggattgt taatcataga ccttgcaaag gtaagtctgt gtttcattcc
     3601 ctttgagaac tctccagctc ttgtgttttc ttttccatca agtcccacaa gctttagcag
     3661 ctccatagga tctcttgtgg gtacatcaaa gagttttcgg taaaaatcaa ggttttcttt
     3721 tgctgttagc ttgctgtata cattggattg ttcaaagctc attccgattt tattaaacat
     3781 atcctttgac atctttctta tatcataacc acctacggtt gcttctcctt cttggagttg
     3841 taaaagtcca gtcaggatat tttgtaccgt tgatttccct gcgcctgagg ggcctaaaaa
     3901 gccaaaaact tctccttttt caatctcaaa actcacatct tttacttggt agatatcatc
     3961 tttattg
//
LOCUS       DASRNA010000085         5119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_330216,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000085 DASRNA010000000
VERSION     DASRNA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330216"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..375
                     /gene="rplU"
                     /locus_tag="VFD08_02365"
     CDS             67..375
                     /gene="rplU"
                     /locus_tag="VFD08_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZK09893.1"
                     /translation="MYAIIETGGQQFKVQEGDEIKIEKLPVEEGQDVVFERVLAIIGE
                     DTNFGKPILEGAKVKATVLEQGRHDKVIIFKYNAKKNFHKKKGHRQPFTLVKINAIEA
                     "
     gene            375..710
                     /locus_tag="VFD08_02370"
     CDS             375..710
                     /locus_tag="VFD08_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HZK09894.1"
                     /translation="MIEVVFEQEDGLLVAFSSKGHAQAGDPGEDIVCAAVSALLFSVV
                     NTLEHYHAMLDVKMGAKGFLRCELKEGYRDKEIQAILQVAKIGISGIVQEYSEFISMD
                     IIDRRIMSC"
     gene            704..982
                     /gene="rpmA"
                     /locus_tag="VFD08_02375"
     CDS             704..982
                     /gene="rpmA"
                     /locus_tag="VFD08_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZK09895.1"
                     /translation="MLRMDLQFFASKKGVGSSRNGRDSNAQRLGVKRGDGCQVNAGSI
                     LVRQRGTKIHPGTNVGRGGDDTLFALTQGVVRFERLGKNRKRVSVYAE"
     gene            1022..2296
                     /gene="obgE"
                     /locus_tag="VFD08_02380"
     CDS             1022..2296
                     /gene="obgE"
                     /locus_tag="VFD08_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZK09896.1"
                     /translation="MYIDKAEIKIIAGKGGNGSASFRREKFEPKGGPDGGDGGRGGNI
                     IARVDLGMHTLMDFRYKRKFVAEDGKNGQKKKCHGRSGKDIYLSFPQGTVIFEKTTGK
                     LLADLSEAEAEVYLARGGRGGKGNVHFKSSTNQAPDYAQRGLEGEEFDLVLELKSIAD
                     VGLVGFPNVGKSSLLSTITKARPKIDSYPFTTLTPNLGVVEVITGKSFIMADIPGIIE
                     GASDGIGLGHDFLRHIERTRLLVHLVDIGISHEEGRSPLEDYQIIRRELESFSEELSK
                     REEVVLLNKLDVMEDESATVELESYLTQRGIKSFRTSTITGQNLAEAMKYITTLLEKI
                     EPIELYSEIEPYEKEEVDREIYFSIVDGEYCVEGQPIEGLMHATDFLSYQSEKRFQVN
                     LKNMGIYDKLKQMGIKEGDTVRIMGYAFEYKE"
     gene            2299..2604
                     /locus_tag="VFD08_02385"
     CDS             2299..2604
                     /locus_tag="VFD08_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HZK09897.1"
                     /translation="MIGKERAYLKKLAHSEKPILQLGKDGLSENFLKELEQTLEMKEL
                     VKLDLLPGSGDKKLMVEEILEKTKAEFISQVGRKLVLYRESSTLPREERLNPARKVR"
     gene            2601..3200
                     /gene="nadD"
                     /locus_tag="VFD08_02390"
     CDS             2601..3200
                     /gene="nadD"
                     /locus_tag="VFD08_02390"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZK09898.1"
                     /translation="MKVGILGGSFDPIHNGHLLMAQYAIEELQLDQVIFLPTFKPPHK
                     MGDKLSKFSDRVNMLELAFDKREDFVLSTLESERPGLGYTYDSLKIFAKKYPGAELFF
                     LIGSDSLMYIEKWYRSEDLLREFFFGVAPRPGRSKKELEATINRLAVDYGAKIYLLDS
                     PYIEISSTGLRQRVKEARPLKYHLPESVEEYIYEQMLYK"
     gene            3178..3735
                     /locus_tag="VFD08_02395"
     CDS             3178..3735
                     /locus_tag="VFD08_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK09899.1"
                     /translation="MSRCSISRANHLAWKYLSHNRYQHSLRVADMAKKLAKRWGAREE
                     FLVRAALLHDLGYSFGGDGLSHAGISGSYSAMVGCDKETVRAIKLHTIGGPAMNLEEK
                     ILFLADGIEMGRSFPRVKEIRKLAFINLDKALLAYLKGSKRYLEKQGKSVHRKTLLME
                     NEIRRRKNGRTDQKNRRLFDRKTSQ"
     gene            3680..4000
                     /gene="rsfS"
                     /locus_tag="VFD08_02400"
     CDS             3680..4000
                     /gene="rsfS"
                     /locus_tag="VFD08_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZK09900.1"
                     /translation="MEELIKKIEDYLIEKQASEVASYDARSGSLADYYIICSALNKRH
                     LNSLAKDVEKFVEKQGVPIFIRQGKADSGWVILDFMDIVIHCFEPETRSFYNLERLWE
                     AMKK"
     gene            3997..4263
                     /locus_tag="VFD08_02405"
     CDS             3997..4263
                     /locus_tag="VFD08_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09901.1"
                     /translation="MKGLRLIGVVLLLFILQLPLVRNAKSLPEIHLFKGDFAQVYSEL
                     KEEFGPWDAEIVLEDGASLKKGKVSFDILRDNYKGSDYLVFINK"
     gene            4278..>5119
                     /locus_tag="VFD08_02410"
     CDS             4278..>5119
                     /locus_tag="VFD08_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZK09902.1"
                     /translation="MEQRKDYLLIKNPTAGTQKSRQWIETLIEELEKQGLTYDLVESS
                     APEHAIRLAYDGAKFYDIIVAMGGDGTVNEVAMGLLLRGEGILGILPVGNGNDFYRIF
                     DDKVDLNLAISKLIEAKTRKINVGRDIEGRYVLNIASVGLDSQTVKMQKKIKKYVPKT
                     MGYVLALIHSMITYRKQDVTIRLDGEEFHSNNVLLCFGTGRSYGGGIKIMPWANIDDG
                     YFNVVNISDISNLGLLLVAPTIIFGTHTKLTKYVKVYKAKEVEIIGKNLTLNLDGDLF
                     DVDKV"
BASE COUNT         1715 a          813 c         1205 g         1386 t
ORIGIN      
        1 agacacgcac gtactaggta ataagcgaca cacctaagcg gcgtaaagat tatgaaggag
       61 gaaaagatgt acgcaattat tgagacaggt ggacaacaat tcaaagtcca agaaggcgat
      121 gaaatcaaaa ttgagaaatt gcctgtagaa gaaggacaag atgtggtttt tgaacgcgtt
      181 ttggccatta tcggcgaaga cactaacttt ggcaaaccca ttcttgaggg agctaaggtt
      241 aaggctactg ttttagagca aggtagacac gacaaggtca ttatttttaa gtacaatgcg
      301 aagaagaact ttcacaagaa aaaaggccat agacagccct ttactcttgt aaaaataaac
      361 gctattgaag cctaatgata gaagtcgttt ttgaacaaga agatggatta ttggtagctt
      421 ttagctccaa gggccatgcc caagctggag atcccggtga agatattgtt tgtgctgctg
      481 ttagtgctct gctctttagt gtagttaata ccttggagca ttatcacgct atgcttgatg
      541 tgaaaatggg agcaaagggt tttttgcgat gtgagctaaa ggaaggatat agggacaaag
      601 aaatccaagc catccttcag gttgcaaaaa tcggcatcag cggaattgtt caagaataca
      661 gtgaatttat ttctatggat attatagata ggaggatcat gtcatgttaa gaatggatct
      721 gcaatttttt gcaagtaaaa aaggagttgg ttcttcaagg aacggtcgcg actctaatgc
      781 acaacgctta ggcgtaaaac gaggagacgg atgccaagtt aacgctggtt ccatattagt
      841 tcgtcagcgc ggcaccaaga ttcacccagg aaccaatgtt ggtcgtggtg gtgatgatac
      901 actttttgct cttacccaag gcgttgtacg ttttgagcgt ttaggtaaga acagaaaacg
      961 agtcagtgtt tatgctgaat aaactcatcc tcagggtgag tttctttttt taggaagtgt
     1021 tatgtatata gataaggcag aaataaaaat tatagcggga aaaggcggta atggtagcgc
     1081 ctcttttcgc agagaaaaat ttgaaccaaa gggtggcccc gacggcggag acggcggtag
     1141 gggaggaaat attattgcta gagtggatct gggaatgcac actctaatgg actttcgcta
     1201 caaaagaaag tttgttgcag aagatggtaa aaacggtcaa aagaaaaaat gccatggtag
     1261 atctggtaag gatatttatt taagtttccc ccagggaact gttatatttg aaaaaaccac
     1321 agggaagctg cttgctgatt tgagtgaagc agaagccgaa gtttatcttg cccgtggtgg
     1381 ccggggagga aagggcaatg tgcactttaa atccagcacc aatcaagcgc ccgactatgc
     1441 tcaaagaggg ctagagggag aagaatttga tctggtgcta gaacttaaaa gtattgccga
     1501 tgtagggctt gttggttttc ctaatgtagg aaaaagctcc ttattatcca ccatcaccaa
     1561 ggccaggcca aagatagata gttatccctt tacgaccttg acacccaatc tgggggtagt
     1621 ggaagtaatc acaggaaaaa gctttatcat ggctgatatt cccggcatta ttgagggagc
     1681 cagtgatgga ataggcttag gccatgattt ccttcgccat atcgaaagaa caagacttct
     1741 tgtgcaccta gttgatatag gaattagtca tgaagaaggt agaagccccc tagaagacta
     1801 tcaaatcatt cgtcgcgaac tagaaagctt tagcgaagaa ctctccaaaa gagaagaagt
     1861 ggtcttatta aataagctgg atgtgatgga agatgaaagt gcaactgttg aactagagag
     1921 ctacttgacc cagaggggga tcaagagctt ccgcacaagt actataacag gacaaaatct
     1981 agcagaagca atgaaatata ttacaaccct tcttgaaaaa atcgaaccaa ttgagcttta
     2041 tagtgaaatt gaaccttatg aaaaagaaga ggtagataga gaaatatact ttagtattgt
     2101 tgatggtgaa tactgtgttg aaggacagcc aatagagggg cttatgcatg ctacagattt
     2161 tttaagctac caatctgaaa aaagatttca ggtcaatcta aaaaacatgg gtatttatga
     2221 taaactaaag caaatgggta ttaaagaagg cgatacagtt cgtattatgg gctatgcctt
     2281 cgaatataag gagtaagaat gattggaaaa gaaagggcct atttaaaaaa actggcccat
     2341 agcgaaaaac cgattttgca actaggtaaa gatgggctct cagaaaactt tttaaaagaa
     2401 ctggagcaga ccttagaaat gaaggagcta gttaagctgg acctccttcc tggttctggt
     2461 gataaaaagc taatggtaga agaaatttta gaaaaaacaa aggcggaatt tattagtcaa
     2521 gttggaagaa aacttgtcct ataccgcgaa agcagcactc ttcctaggga agaacgatta
     2581 aatccagcta ggaaagtaag atgaaagttg gaattttagg tggcagtttt gatcccattc
     2641 ataatggcca ccttcttatg gcccaatatg caatagaaga gctgcaattg gaccaggtaa
     2701 tttttttacc aacttttaaa ccaccccata aaatggggga taaactttcg aaattttcag
     2761 acagagtcaa tatgcttgaa cttgcctttg ataaaagaga ggattttgtc ctatctactc
     2821 ttgaaagcga gcgccctggc ctgggttata cctatgactc tttaaaaata tttgccaaaa
     2881 agtatccagg agctgaactt tttttcctta ttggttctga tagtttaatg tatatagaaa
     2941 aatggtaccg gtctgaggat cttttaaggg agttcttttt tggagtggcc cctagacctg
     3001 gtcggtccaa aaaagagttg gaggctacaa taaataggct tgcagtggat tatggtgcca
     3061 agatatatct tttggattca ccctatatag aaatttcttc cactgggctt cgtcaacgag
     3121 taaaagaagc aaggccccta aaataccacc tacctgaaag tgtggaggag tatatttatg
     3181 agcagatgct ctataagtag ggccaatcac ctagcttgga aatatttaag ccataatcgt
     3241 tatcaacaca gtctaagagt agcagatatg gccaaaaaac tggcaaaacg atggggtgct
     3301 agggaagaat ttcttgttcg agcggccctt ttacacgatc ttggatatag ctttggtggt
     3361 gatggactaa gtcacgctgg aataagtgga agctatagcg ctatggttgg ttgtgataaa
     3421 gaaacggtta gagccataaa acttcacacc attggtggac cagctatgaa cctggaagaa
     3481 aagattcttt ttcttgccga tggaattgag atgggaagat cttttcccag ggtaaaagaa
     3541 ataagaaaac ttgcctttat caatctggac aaggcgttac ttgcttattt aaagggatca
     3601 aaaagatatc tagaaaaaca aggaaaaagt gtccatagga aaacccttct tatggaaaat
     3661 gaaattagaa ggaggaaaaa tggaagaact gatcaaaaaa atagaagatt atttgatcga
     3721 aaaacaagcc agtgaggttg caagttacga tgccaggagt ggatctttag ccgactatta
     3781 tataatttgt agtgcattaa acaaaagaca tcttaattct ttggcaaagg atgtagagaa
     3841 gtttgtagaa aaacagggtg ttccaatttt tatccgtcag ggaaaagccg atagtggttg
     3901 ggttatttta gactttatgg atattgtaat ccactgtttt gagccagaaa caagaagttt
     3961 ttataatctt gagaggctct gggaggcgat gaaaaagtga aagggttgcg cctaataggt
     4021 gtagttcttc tgctttttat tttgcaacta ccacttgtaa gaaatgcaaa aagcctacca
     4081 gagattcatt tgtttaaggg ggattttgct caggtctata gtgagcttaa agaagaattt
     4141 ggcccctggg atgctgagat tgtcttagaa gatggagcaa gtcttaaaaa gggaaaggtc
     4201 tcctttgata tccttagaga taattacaaa ggatctgatt acctggtgtt tataaataaa
     4261 taatcaaggg gattactatg gagcaaagaa aagactatct gcttattaaa aatccaactg
     4321 ctggcacgca aaaaagccgt cagtggatag agaccttaat tgaagaatta gaaaaacagg
     4381 gcctaactta tgacttggtg gagtcatcag cccctgagca cgctataaga ctggcctatg
     4441 atggggctaa gttttacgat ataattgtag ctatgggcgg ggatggtact gttaatgaag
     4501 tagccatggg cttgcttctt aggggtgaag gaatacttgg aattttacct gtaggcaatg
     4561 gaaatgattt ttatagaatt tttgatgata aggttgatct taatctagcc ataagcaagt
     4621 taattgaggc caaaacaaga aaaatcaatg taggtagaga tatagaagga agatacgtac
     4681 tcaatattgc cagtgtaggc ctagatagcc aaacagttaa aatgcaaaaa aagataaaaa
     4741 aatatgtacc aaagaccatg ggatatgttt tagccctgat tcactccatg atcacctacc
     4801 gaaaacaaga tgtaaccatt cgcttagatg gagaggaatt tcatagcaat aatgtgcttt
     4861 tgtgttttgg aacaggtaga agttatgggg gtggcattaa aatcatgccc tgggcaaaca
     4921 ttgatgatgg atattttaat gtagtgaata taagcgacat ttcaaatctt ggactcttat
     4981 tggtggctcc tactattatt tttggtactc atacaaaact tacaaaatac gtaaaagtat
     5041 ataaagcaaa agaagttgaa attataggga aaaatttaac cttaaattta gacggagacc
     5101 tttttgatgt agacaaggt
//
LOCUS       DASRNA010000086         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_844891,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000086 DASRNA010000000
VERSION     DASRNA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844891"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1015)
                     /locus_tag="VFD08_02415"
     CDS             complement(80..1015)
                     /locus_tag="VFD08_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZK09903.1"
                     /translation="MKKILVTGATGQIGTDLVERLIEVYGDDNVMSCSRRKRPDKWVG
                     SHYESLDVTDAKGFYDLTKNFGADTVLHLAAVLSAVGEGNPQLLWDINVNGLYNALEV
                     SRELDLALFVPSSIGAFGPSTPKTSTPQTTIQRPTTAYGVSKVAGELLCDYYHKRYGL
                     DTRGVRFPGLISYKALPGGGTTDYAVHIYYDALEKGHYTSFIGKGTYMDMMYMPDAVE
                     CILDLMEANPDKLIDRNAFNVSGMSFEPEEIKKSIQKHLPDFVLDYDVDPARQAIADS
                     WPDSLDVSAAKAQWGFNPKYDLDAMTKDMLEKLAK"
     gene            complement(1027..2409)
                     /gene="asnS"
                     /locus_tag="VFD08_02420"
     CDS             complement(1027..2409)
                     /gene="asnS"
                     /locus_tag="VFD08_02420"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZK09904.1"
                     /translation="MTCKELYKDYALFEGKEINLQGWVKRKRTSANVGFFELSDGSMF
                     ETIQIVFDKEVENFNELDHLGVQSCIEVRGELVLTPNTKQPFEIHAQKIDVLFEADKS
                     YPLQTKRHSMEFLRTILHLRPRTNTFMAVFRVRSLISHAIHLFFQEKDFVYIHTPIIT
                     GTDAEGAGELFRVTTLEDEEIFENKDQKVDYSQDFFGKETFLTVSGQLSVEPFALSFG
                     NVYTFGPTFRSENSNTARHASEFWMVEPEMSFCDLECNMQIAEEMMKYIVSYVLENAP
                     SEMDFFNRFVDKGLIKRLEALVKEPFAQTTYTEAIDILLKSGREFSYPVEWGCDLKTE
                     HERFLAEDYFGKPVFVTDYPSEIKAFYMKRNPDGKTVRAMDLLVPGVGEIIGGSQRED
                     DYDTLKAAIEERGMDMENYQWYLDLRRYGSCVHSGYGLGLERAVQYITGMGNIRDVIA
                     YPRTPGNAEF"
BASE COUNT          793 a          610 c          457 g          837 t
ORIGIN      
        1 ctattagtgg tcctttcatt ttatctcctt agaaggattt caatcccttt taatagaaaa
       61 actagcaggc aaagactttt tacttggcaa gcttttctaa catatctttt gtcatggcat
      121 ctaaatcata ttttggatta aatccccact gcgctttagc agcagaaaca tctaaactat
      181 ctggccatga gtctgcaatt gcttggcgag caggatcaac atcatagtca aggacaaagt
      241 caggcagatg tttttggatg ctttttttga tttcttctgg ttcaaagctc atccctgaga
      301 cattaaaagc atttctatca atcagtttat caggatttgc ttccataaga tcaagaatgc
      361 attcaacagc atcaggcata tacatcatat ccatataggt tccttttcca ataaatgaag
      421 tatagtgtcc tttttcaaga gcgtcatagt aaatatgtac cgcataatca gttgttccgc
      481 cccctggtag ggccttatag gaaataagtc ctggaaaacg cactccacga gtatcaagac
      541 catagcgctt atgataataa tcacagagga gttcgccagc taccttacta acaccatagg
      601 ctgttgtagg acgctgaatc gttgtttggg gtgtgcttgt tttgggtgta cttggtccaa
      661 aggcgccaat agaacttgga acaaaaagag ccaaatcaag ttctctagaa acttctaagg
      721 cattataaag accatttaca ttaatgtccc aaagaagttg aggattaccc tcgcccacag
      781 ccgaaagaac tgctgctaaa tgaagcaccg tatctgcacc aaagtttttg gttaaatcat
      841 aaaaaccctt tgcatcggtt acatcaaggg actcataatg acttcccacc cacttatctg
      901 ggcgttttct tcttgaacaa gacattacat tatcatcacc atacacttcg atgagtcttt
      961 caaccaaatc tgtaccaatt tgccctgttg ctcctgtaac taaaatcttc ttcatttttt
     1021 ctccttttaa aattctgcat tccccggcgt tcgcggatag gctataacat cacgaatatt
     1081 gcccattcca gttatatact gaactgcacg ttctagtccc aaaccataac ctgaatgaac
     1141 acaagatccg tagcggcgca agtctagata ccattgataa ttttccatat ccatgccacg
     1201 ctcttctatg gcagctttga gcgtatcata gtcatcttct ctttgagaac caccgataat
     1261 ttctcccaca ccaggcacaa gtaggtccat ggcccgaact gtttttccat cgggattgcg
     1321 cttcatataa aaagccttaa tctcagaggg ataatcagta acaaatactg gtttgccaaa
     1381 atagtcttct gctaaaaatc gttcgtgttc tgttttcaga tcacatcccc actctacggg
     1441 ataggaaaac tctcgtccgc tttttagcaa aatatcaatt gcttcagtat aggtggtctg
     1501 ggcaaagggc tctttaacaa gagcttccaa ccttttaata aggcctttat caacaaaacg
     1561 attaaagaag tccatttcac tgggagcatt ctctaaaaca tagctaacga tatatttcat
     1621 catttcttct gcaatttgca tattgcactc aagatcacaa aaactcattt caggctcaac
     1681 catccagaat tctgaggcgt gacgcgctgt attggagttt tctgagcgaa aggttggacc
     1741 aaaggtatat acattgccaa agcttagtgc aaagggctca acagataact ggcccgaaac
     1801 cgtaagaaaa gtttcttttc caaaaaaatc ctgagaataa tcaacctttt gatctttatt
     1861 ttcaaaaatt tcctcgtcct ctagggttgt tactctaaat agctcccctg ccccctcggc
     1921 atctgttcca gtaataatgg gggtatgtat atagacaaaa tccttttctt gaaaaaacaa
     1981 atggattgca tggctaatca agctccgcac tcgaaaaaca gccataaaag tatttgttct
     2041 tggtcttaga tgaaggattg ttcgtaaaaa ctccatactg tgcctttttg tttgcagagg
     2101 atagctttta tctgcttcaa ataaaacatc aatcttttga gcatgtattt caaagggctg
     2161 tttggtatta ggggttaaaa ctagctcgcc tcttacttca atgcaagatt ggaccccaag
     2221 atgatcgagc tcattaaagt tctccacttc cttatcaaaa acaatctgaa ttgtctcaaa
     2281 catggaaccg tcacttaatt caaaaaagcc tacatttgca ctggttcttt ttcttttaac
     2341 ccagccctgt agatttattt ctttgccttc aaataaggca taatctttat aaagttcttt
     2401 acatgtcatc ctttcctcct cataaaaaaa gactttcatc caagggacga aaagtcgtgg
     2461 tgccacccta atttttgctt tttctgcgat aagggacaga ccccctagga ctcctgggtg
     2521 tttttcccct tttttttcac atcaggcttc cactgtccct gactcgctag agcttccaaa
     2581 aaggatactc ttccattcat catccgagta aagtatagct attttatcat taattgtcaa
     2641 caaaatgcaa atgcaaataa gaaagaaatt ctttcgttat ttgatccgtc tttgccc
//
LOCUS       DASRNA010000087         3133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_471440,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000087 DASRNA010000000
VERSION     DASRNA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471440"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFD08_02425"
     CDS             <1..827
                     /locus_tag="VFD08_02425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZK09905.1"
                     /translation="DKTEVKVEMKSVNSRFLEINMRMPRKLLAYEIDLQNLLKKKLNR
                     GKVEVRISMEEPFSQGKDFEIDYGMIDALKELIASGKLPLEEKFTLRDLLALEGAVVL
                     KDKAEADYEEPLKEGFLQALDNLLLSREREGEALKNHMTRLLYELESHLKLIREKMPL
                     WKKTYSENFRNRLMELLEDKSLIQEERLEFELALFSEKKDIEEEIQRALTHVEAFKRD
                     LDQAGPHGRKLDFLVQELLREINTMGSKSAAYDLTKEVIEAKTVLEQIREQLQNVE"
     gene            830..1450
                     /gene="gmk"
                     /locus_tag="VFD08_02430"
     CDS             830..1450
                     /gene="gmk"
                     /locus_tag="VFD08_02430"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZK09906.1"
                     /translation="MYKKRGRVFVISGPSGVGKGTLVKETLKRKNLHLSISYTSRKPR
                     FNEEEDQSYYFIDKDKFKKMINEGVFAEWAEVYGNYYGTPYAELEKHLVRGEDVILEI
                     EMIGAAQIKSMGEDAVLVFVLPPSLAMLKERLKKRGTEKEEAIAKRLSCTMDELKHIK
                     DYDYYLVNNDIDKATEQLLAIIEAENHRVSRAFLDYLETSEREFSL"
     gene            1450..>3133
                     /locus_tag="VFD08_02435"
     CDS             1450..>3133
                     /locus_tag="VFD08_02435"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09907.1"
                     /translation="MYYKIILLDAVSDYDRPYVYQSQKNLEKGQLVLVPFGRGNQPRE
                     GLVFGEAAEFSKAKNILEDTGIFLSPISLAAIDFLSSYYAMPPAAYSRLFLPKALRVK
                     PKLHLVMREDLNEKEIKEYLTIKDDKEKLGQSGLCHEEYLYDQRVKLPEEEMLLSQYS
                     FIDLKAYLETLPKNYKRQKEIIENLLNHFPSMVPREGASLSSIKSLEKKGILTRVFIP
                     RRITSNYKKVKENYKDLPLTKPSENKLVKEINASAKGLYLLLRQGSSARNVFLHLAAR
                     SLEKGKRFLLLFPDASLIPAAVELFKEHFGDLVGVYHGKMTIKEQKSELLSFKEGRFP
                     IMITSRIGLFLPLEDVDTMILDECQDDGYVSQNPYFHGNELASFYAQQHGIKTVFASS
                     APPLDLYLREDIQKLILEEDSFSHPQGYLVDMRRELSEGNRSPLSKGMIKEIDQLLEN
                     NGLAFLLMNKRNYASYVFCRKCGHALYCPHCRASLHYDQKNKKLFCPACGYKSPSVRD
                     CPECGSKAFRFYGGGIQMVSEELKRLYPKARILSVDAKTMEQKDGYIKLHRIL"
BASE COUNT         1045 a          529 c          654 g          905 t
ORIGIN      
        1 ttgacaaaac cgaagtcaaa gtagagatga agtctgttaa ctctagattt ttagaaataa
       61 atatgcgtat gccaagaaag cttctcgcct atgaaattga tcttcagaat cttcttaaaa
      121 agaagcttaa taggggaaag gtggaggtaa gaattagcat ggaggaacct ttttcccaag
      181 gaaaagattt tgagatcgat tatggcatga ttgatgcatt aaaagaactc attgcctctg
      241 gcaaattacc cctagaggaa aaatttaccc taagagatct tctagcgcta gagggggctg
      301 tggttcttaa ggataaggct gaggctgatt atgaagagcc actcaaggaa gggtttttac
      361 aagccctaga caatttgtta ctttcgcgag aaagagaagg ggaggcctta aaaaaccata
      421 tgactcgttt gttatatgaa ttggaatctc acctaaagct gattcgtgaa aaaatgccct
      481 tgtggaaaaa aacttattca gaaaattttc gaaatcgtct tatggaactt ctagaagaca
      541 aaagcttaat tcaagaggaa cgcttggaat ttgagcttgc actcttttct gaaaaaaaag
      601 atatcgaaga agaaatccaa agggcgctta cccatgtgga agcctttaag agggatctgg
      661 accaagcagg cccccatggc agaaaactag actttcttgt tcaagaactc ttacgggaga
      721 tcaatacgat gggttctaaa tcggctgcct atgatcttac aaaagaagtg atagaggcaa
      781 aaaccgtgct agaacagatt cgcgaacaat tacaaaatgt ggagtaaata tgtataaaaa
      841 aagaggaaga gtctttgtta ttagtggtcc ttccggtgta ggaaagggaa ccttggttaa
      901 agaaacacta aagcgcaaaa atttacatct tagtatttcc tatacatcaa gaaaaccccg
      961 ttttaatgaa gaagaggacc agagctatta ttttatagat aaagataaat ttaaaaaaat
     1021 gattaatgag ggtgtttttg ctgagtgggc agaggtctat ggcaactatt atggcactcc
     1081 ctatgcagaa ctagaaaaac atttagttcg tggagaagat gttattcttg aaattgagat
     1141 gattggggct gcacaaatca aatcaatggg agaagatgct gtgctggtat ttgttcttcc
     1201 tccaagtctt gctatgctta aagaacgatt aaaaaagcga ggaacagaaa aagaagaagc
     1261 catagccaaa agattgagtt gtactatgga tgagcttaag catatcaaag actatgatta
     1321 ctatcttgtt aataatgata tagataaggc aactgagcag ttactagcta ttattgaagc
     1381 ggaaaaccat cgagtttccc gtgccttttt agactactta gagacatccg aaagggaatt
     1441 ttcactttag tgtattataa aattattctt cttgatgcgg tcagcgacta tgatcgcccc
     1501 tatgtttacc aaagtcaaaa aaacctagaa aaaggccaac ttgtattggt tccctttggc
     1561 cggggcaacc agcccagaga agggcttgtt tttggtgaag cagctgagtt ttctaaggca
     1621 aaaaatattc tggaagacac aggtatattt ttaagcccta ttagtcttgc tgccattgat
     1681 tttcttagtt cttactatgc tatgcccccc gctgcctata gccggctttt tttacctaaa
     1741 gcgcttagag ttaagcctaa acttcatcta gtaatgagag aagatcttaa tgaaaaagaa
     1801 ataaaagagt atttaactat taaagatgac aaagaaaaac tcggtcaatc gggtctatgt
     1861 cacgaagaat acttgtatga tcaaagagtc aagttacctg aggaggagat gcttctttct
     1921 caatattcct ttatcgattt aaaagcatat ttagagactc ttcccaaaaa ctataaaaga
     1981 caaaaagaga ttatagaaaa tctactaaat cattttccaa gtatggtccc tagagaagga
     2041 gctagtctat ctagtatcaa atcacttgag aaaaaaggaa ttcttactcg agtctttatt
     2101 ccccggcgca ttacaagtaa ttataaaaaa gtcaaagaaa attataaaga cttaccttta
     2161 acaaagccta gtgagaataa gctggtaaaa gaaatcaatg cctctgccaa agggctctat
     2221 cttttactta gacaaggttc ttcagctagg aatgtttttt tacatttagc ggccaggagt
     2281 cttgaaaagg gaaaacgttt tcttctctta tttcccgatg catcacttat acctgctgct
     2341 gtagaactgt ttaaagagca ttttggtgat cttgtaggtg tctatcatgg aaaaatgacc
     2401 attaaagaac aaaaaagcga actcctttca tttaaagaag gtcgttttcc tattatgatc
     2461 accagtagaa ttggcctctt tttaccctta gaggatgtgg ataccatgat cctcgatgag
     2521 tgccaggatg atggttatgt ttcacaaaac ccgtattttc atggcaatga gttagcttca
     2581 ttttatgccc agcagcatgg aattaaaacg gtttttgcct catctgcgcc tcctttggat
     2641 ctttatctta gggaggatat tcaaaagctg atcttagagg aagattcctt ttctcatccc
     2701 cagggatatt tggtagatat gagaagagaa ttatctgagg gcaatcgcag tcctctaagt
     2761 aagggtatga ttaaggaaat agaccaactc ttagaaaata atggtttagc ctttctttta
     2821 atgaataaaa gaaattatgc aagttatgtc ttttgccgaa aatgcgggca cgccctgtat
     2881 tgtccccact gtcgggccag tcttcattat gatcaaaaaa acaagaaatt attttgtccg
     2941 gcctgtgggt ataaaagccc ttctgtaaga gactgccctg aatgtggaag taaagccttc
     3001 cgtttttatg gcgggggcat acaaatggtc agtgaggaat taaagcgttt gtatccaaaa
     3061 gctcgtatac ttagtgtaga tgctaagact atggagcaaa aagatgggta tataaagtta
     3121 cataggattt taa
//
LOCUS       DASRNA010000088         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_247305,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000088 DASRNA010000000
VERSION     DASRNA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247305"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..461)
                     /gene="ssrA"
                     /locus_tag="VFD08_02440"
     tmRNA           complement(110..461)
                     /gene="ssrA"
                     /locus_tag="VFD08_02440"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(462..902)
                     /gene="smpB"
                     /locus_tag="VFD08_02445"
     CDS             complement(462..902)
                     /gene="smpB"
                     /locus_tag="VFD08_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZK09908.1"
                     /translation="MKILSNNRRARFEYEILEEFEAGLVLVGTEVKSIKEGKVNIREA
                     YIEIRDGEAFITGMHVAPFREGNLRNVDPYRERKLLLNRREINKLYKEVTQAGLTIVP
                     LRLYRNNKGLVKIEIALARGKKLYDKREAQKEKDLKRQMERYQI"
     gene            1001..1074
                     /locus_tag="VFD08_02450"
     tRNA            1001..1074
                     /locus_tag="VFD08_02450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1033..1035,aa:Gly,seq:ccc)
     gene            1122..1604
                     /locus_tag="VFD08_02455"
     CDS             1122..1604
                     /locus_tag="VFD08_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK09909.1"
                     /translation="MKLIPAKVEDIPWIMMLEKAPENRNFVWQGSYEEHLFEINDPDS
                     YLLIHLDKSDKNIGYSLNKFHKKSKNFEIRRIVVNNKGLGLGKKSMKALLDFGFNELG
                     AQRIWLDVYPENEIGISLYKSLGFTQEAHLRRSDYQRGKYYDQLVFGLLKEEYQELLL
                     "
     gene            1688..1775
                     /locus_tag="VFD08_02460"
     tRNA            1688..1775
                     /locus_tag="VFD08_02460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1722..1724,aa:Ser,seq:cga)
BASE COUNT          738 a          527 c          433 g          814 t
ORIGIN      
        1 atatggaaaa atcacgcttt ttttcgtgat ttttcaaaaa aacgaaagct tgaaagcatt
       61 gaaattgcaa tgaaaaagag cccttgaatt ttcaaaggct cttttttgat ggtggagatg
      121 aggggaatcg aacccctgtc caaaaacaaa ttacttcagg cttctacgag tgtagttgtt
      181 ttttataacc tatccttaca aaacaacaaa ctagcaatag gtttgcctct aaattaccct
      241 agagcagcga ggcaccactc tattcctgcc actgattgat gtcctctatc cggcgtcact
      301 ggcgaaggcc ctggatagaa gagctgcgtt aattacgcag cgtaagcaaa attatcgttt
      361 gcgtttaaat agattctccc agttctttaa cgtgtacccc aagagaaaaa cacgactcgc
      421 ttccctgagc tttctatcct tgtcgaaacc tgtacatccc cttatatttg atagcgctcc
      481 atttgtcttt taagatcttt ttctttttga gcttctcttt tatcgtagag ttttttaccc
      541 ctggcgagcg caatctctat tttaacaagg cctttattat tcctatataa tcttaaggga
      601 acaattgtaa gacctgcctg ggtaacctct ttatagagtt tattgatctc tctgcgattt
      661 aatagcagtt ttctttcacg gtatgggtca acatttctta agttaccctc tctaaaggga
      721 gctacatgca tcccagtaat aaaggcttct ccatctcgta tttcgatata ggcttccctt
      781 atattaacct ttccttcttt aatagactta acttcagtcc ctacaagaac taggcctgct
      841 tcaaactctt ctagtatttc atattcaaaa cgtgcccttc ggttattact tagtattttc
      901 attgcccctc cgcacctcta ttatagccta ttttgaggga gaaatcaatc ttttccttga
      961 cagctcaccc actaggggtc tacaatagtt tcatagaatc gcgggtgtag ctcagtggta
     1021 gagccctggc ttcccaagcc agtcgcgagg gttcgattcc cttcactcgc tccaatatgt
     1081 gtaagccttc gcttacattt tttttgaacc aggaggttaa aatgaagcta attcctgcaa
     1141 aagttgaaga tatcccttgg attatgatgc tagaaaaagc tcctgagaac cgtaattttg
     1201 tttggcaggg tagctatgaa gaacatcttt ttgaaataaa tgatccagat agctatctat
     1261 taatccacct agataaatca gacaaaaata ttggttattc cttaaataaa ttccataaaa
     1321 agagcaagaa tttcgagatt cgaagaattg tagtaaacaa taaaggatta ggattgggta
     1381 aaaaatcaat gaaggcactg cttgattttg gctttaatga actaggggcc caacgcatat
     1441 ggcttgatgt ctatcctgaa aatgaaattg gcatttcttt atataaatcc ctaggcttta
     1501 cccaggaagc ccatttaagg cgcagtgact accagcgcgg taaatattat gaccaacttg
     1561 tctttggtct tctcaaagaa gaataccaag agctcttatt ataagctagc accccccaag
     1621 ctggcaatta tcagcaagat ttataaacta gcaatacaat gaaatagatt taactttgcc
     1681 ttgttttgga gggttgtccg agaggttgaa ggtgcgacac tcgaaatgtc gtgtagagaa
     1741 atctacctag ggttcgaatc cctaatcctc cgccaagaaa agcagttttt tactatttgt
     1801 agaaaactgc ttttatatta tacccttatt ccattgcttt atctgagcac tcttaagtta
     1861 tcggtctttt tatcctctga cttctaaatt atcccttatc gtatacactg tcagtataag
     1921 acactatata aaagctattc tactagagca aaaattataa atcctatctt gtcttgcttt
     1981 ttcagccatc ttctctcctt ttttttctag aaaggatcat tacacccata aaaaggagaa
     2041 aaaacccatt ccctagtttg acaccgattt actaatcttc aaatctattt gcttgatcgc
     2101 tgcaattctt agcaactttt ttcttgtcaa ttttaaatct ttttaatgta cttctatgtg
     2161 tttctgtggt gtaattataa aaagtggagg tgcatcatgt atctaaaaaa agacaagcgt
     2221 ccaaacaaaa aagtctgcct ttccatcgtc aaatccttta gagatccaat taccaaaaag
     2281 tccaggtaaa gggatataat tagtgtgggt ttttttgatg aacttacaga tcagtatacc
     2341 gatccgatca cccacttcga tcagctacca aggaaaatga cttaggagga gaatgcttcc
     2401 cagcagcccc tcacttttag ctttgataaa gaggagctcc taaaccggga ggatagacta
     2461 cgtaaaaagc tatgctttct ctctctttct tttctctatc ataagctaga gt
//
LOCUS       DASRNA010000089         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_247836,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000089 DASRNA010000000
VERSION     DASRNA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247836"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFD08_02465"
     CDS             complement(<1..392)
                     /locus_tag="VFD08_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09910.1"
                     /translation="MVEIGNTGIYRPNVEIPSGQVILFKGKEYQLIELVNKNGNININ
                     TDKDKDIKDNLKEGSLYIPAGRDARGREVTITAKGDIISESDLHSQGKLKVISEKGQI
                     IINDAEVNSSHGELEMKGRAGISAQGAN"
     gene            complement(618..1229)
                     /locus_tag="VFD08_02470"
     CDS             complement(618..1229)
                     /locus_tag="VFD08_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09911.1"
                     /translation="MKHKKLIILLLIVSVFFGLSIFSPTLARYVSEYQGSDRAMVARW
                     DFKVDTDAAGLDDFDLFDGAELGPQASGQNSFIISPGESDVAIDYLVYMNTEVLRLSD
                     DKYLPIVFRIASSSGQSMIKDWFAPIDKLDYFQVVEGIFEAGSNASDEITIEWKWDTS
                     DYRGEATEKEYNNQVLEAVKDLGPEFKGNITFKIEGKQIAPSR"
     gene            complement(1310..1876)
                     /locus_tag="VFD08_02475"
     CDS             complement(1310..1876)
                     /locus_tag="VFD08_02475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZK09912.1"
                     /translation="MESYNENQAEKRDKYLKKNWKKITVNSLKYMFFAFMGLVLFINL
                     YIGVCSAVTKEAVPQCFGITPLVVLSGSMEPAIMPGDVVIVQKQDIDKYKEGDVATYL
                     AGNVAYTHRIIAKEEGVFVFKGDSNTLVDSEVEATQLIGRVILRIPKIGVAILFFKTP
                     PGMLLLVLIFILALYGDEIFRKIKKRND"
     gene            complement(1994..2659)
                     /locus_tag="VFD08_02480"
     CDS             complement(1994..2659)
                     /locus_tag="VFD08_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09913.1"
                     /translation="MIKIVTKEKGELIMENKNNNKRTYRLGILLLLFTLLSTVMLPGT
                     FAKYTSEYAGSDTALVAKWDIDITDGDKSIIVGETLNLDLFSHNFNKNILSSAGSEKI
                     VAPGVDGSFKLEVNNASDVAAKISYTLEKTSGSADVPMEFSIDDGKNWLSLEELNASL
                     NTPVNIAGVGSGTVEKTVGWKWAFEGNDANDTALGVASAETSRTNYALNVKVTATQVM
                     PTE"
     gene            complement(2734..>3295)
                     /locus_tag="VFD08_02485"
     CDS             complement(2734..>3295)
                     /locus_tag="VFD08_02485"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZK09914.1"
                     /translation="VIIVNGTMIVRSFLYPDELPDFIGYKPLIVLSGSMEPTMEAGDL
                     ALVRKTPAEKIAKDDVIAFRLDKSTETAIIHRVTEVVSENGEMTFFTKGDANTGTDEE
                     PVKAENLEGRYLGKIAKLGHFALFLQTPMGMLLFVIIPLALLLGYDVIFRSKDKSARE
                     VELEAQLAELKQEQKKAKDHKEDKTK"
BASE COUNT          938 a          717 c          497 g         1143 t
ORIGIN      
        1 atattagccc cttgggcgct aatgcccgcc ctccccttca tttctaactc accatgagag
       61 gaatttactt cggcatcgtt gataattatc tgacccttct cacttataac tttgagcttt
      121 ccttgactat gaagatcaga ttctgatatt atatccccct ttgctgtaat ggtaacctct
      181 ctacctctag catccctgcc tgctggaatg tacaatgagc cttcttttag attgtcctta
      241 atatctttat ctttatcagt atttatattt atatttccgt ttttgtttac aagttctatt
      301 agttgatact ccttgccctt aaacaagata acttgcccag aaggaatctc aacatttggt
      361 cgatagatcc ctgtgttgcc tatttccacc atttcatcct ctgactcatc tggaatttta
      421 acactgatat tgttactagt ggacaatgaa atagtagaag cataacgaga ataagtggta
      481 cctgtagaaa gtaccactaa taatataagt aaaaataaga tatataagaa aatttgtttg
      541 aatttcaagt tgtttattat ggtttttatt ttatgtcact ccctataagt caaatatcta
      601 aaaatgattt tcgtcagtta tctgctgggt gcaatctgtt tgccctctat tttaaaagta
      661 atatttcctt taaactctgg tcctaagtcc tttacggctt ctaggacctg gttattatat
      721 tctttttcag tggcttcacc cctgtaatcc gaagtgtccc acttccattc tattgtgatt
      781 tcatcacttg cattcgagcc agcttcaaag attccttcta ccacttggaa gtagtctagc
      841 ttatcaattg gagcaaacca gtctttaatc attgactgcc ccgatgagct agcaatacga
      901 aatacaatag gaaggtattt atcatcacta agcctcagca cttctgtatt catataaaca
      961 aggtagtcta tagcaacatc tgactctcca ggagaaatta taaagctatt ttgcccactt
     1021 gcttgtggtc ccagttctgc tccatcaaat aagtcaaaat catccaagcc agcagcatct
     1081 gtatctacct taaagtccca tctggcaacc atagctctat cagagccctg atactcgctt
     1141 acatatcgag ccaaagttgg cgaaaagata cttagaccaa aaaacactga tacaatcagc
     1201 aaaagtataa ttagtttttt gtgtttcatc tctacccatc ttctctcttt ttatttttta
     1261 tgcatcaaat ttcttttaga agctccatct tattatcaag acttgttttt taatcattgc
     1321 gcttttttat ttttcggaag atctcatccc catataaagc tagaataaaa ataagcacca
     1381 gtaaaagcat accaggtggg gttttaaaga acaaaatagc tacaccgatc tttggtattc
     1441 tcaaaataac tctacctatt agctgtgtag cttctacttc actatcaaca agagtattac
     1501 tatcgccttt aaatacaaat acaccctctt cttttgcaat gattcggtgg gtataggcca
     1561 catttcctgc tagataggta gccacatctc cctctttgta tttatctata tcttgctttt
     1621 ggacaatcac tacatcacct ggcataatag ctggttccat agagcctgat aatacaacaa
     1681 ggggggtgat cccaaaacac tgaggtaccg cttccttggt taccgctgaa caaactccta
     1741 tatataaatt aataaatagg accaggccca taaaagcaaa aaacatgtat tttaaagaat
     1801 ttactgttat tttcttccag ttttttttca aatatttatc tcttttttct gcctgatttt
     1861 cattgtagct ttccaaaaaa gcctacttca ggctgccttg cctagagcac cactcttgat
     1921 ggcctaggca agagagcctg agctctctta cttaaaactt taatttcgtt tatagatgtt
     1981 aatcagtctt tgattactca gttggcataa cctgagtagc tgttaccttt acattaagag
     2041 catagtttgt gcgtgaggtt tcagcagaag caacaccaag tgcagtgtca ttagcatcat
     2101 taccctcgaa tgcccatttc caaccaactg ttttttctac agtgccagat ccaaccccgg
     2161 ctatatttac cggagtattt agactagcat ttaactcttc caaactcaac caattctttc
     2221 cgtcatcaat tgaaaattcc attggaacat cagcacttcc gcttgttttt tcaagtgtat
     2281 aggaaatttt agcagcaaca tctgaagcat tgtttacttc tagcttaaaa ctaccatcaa
     2341 caccaggtgc aacaattttt tcgctgcctg cactgctaag aatgttctta ttaaaattat
     2401 ggctaaataa atctagattt aatgtttctc caacaataat agatttatct ccatctgtga
     2461 tatcaatatc ccactttgct accagggctg tatcgctacc ggcatactcg cttgtatatt
     2521 tagcaaatgt tcctggaagc ataactgttg ataatagggt aaatagcaaa agcaagatcc
     2581 ccagtctata agttcttttg ttgttgtttt tgttttccat aattaattct cctttttctt
     2641 ttgttactat ttttatcatt ctgatcccgt tcataatttg tatttataag aactatcaga
     2701 ccgaatgatt attaaaacgt ttacaagttg ctgtcattta gttttgtctt ctttatgatc
     2761 ttttgctttt ttctgctctt gctttagttc tgctagttga gcttctaatt ccacttccct
     2821 agcacttttg tctttactgc ggaatattac atcatagcct aggagtaggg caagaggaat
     2881 gataacaaag agtaacatac ccatgggggt ctgtaagaaa agggcaaaat gacctaattt
     2941 agcaattttt cctaaatacc taccctctaa gttttctgct tttacgggtt cttcatctgt
     3001 accggtattt gcatcaccct tggtaaaaaa tgtcatttct ccattttcac ttaccacctc
     3061 tgttaccctg tgaataatgg ctgtctccgt actcttatcc aaacgaaaag caataacatc
     3121 atcctttgca atcttttcag caggagtttt tcttacaagg gccagatctc ccgcttccat
     3181 agttggctcc atagaacccg ataaaacaat tagcggctta taacctataa agtctggtag
     3241 ctcatctgga taaagaaaag atcttacgat catcgttcca ttaacaataa ttact
//
LOCUS       DASRNA010000090         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_397886,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000090 DASRNA010000000
VERSION     DASRNA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397886"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFD08_02490"
     CDS             <1..440
                     /locus_tag="VFD08_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HZK09915.1"
                     /translation="IFDDYEKLLGLDTFKKLFPVLLTDRGSEFTDPTAVEFSPFTGER
                     RSYVFYCDPQRADQKGAIENCHSLARRMIPKSEDLRPWTQEIINEVGDHLNSYPRKKL
                     MDKSPYDSFSFFLGDNISNLLHWKKIPSKDIQLNPHYFRNKLK"
     gene            complement(753..2465)
                     /locus_tag="VFD08_02495"
     CDS             complement(753..2465)
                     /locus_tag="VFD08_02495"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZK09916.1"
                     /translation="MKDNKTKDKKKTSNNPMDKNGLSESTPSRNQNTGENPYITTAAG
                     APVTDNHDSMTAGKRGPMTLEDVWFLEKMAHFDREVIPERRMHAKGSGAFGTFTVTHD
                     ITKYTRASIFSEVGKTTQMLARFSTVAGERGAADAERDIRGFAMKFYTEEGNWDLVGN
                     NTPVFFFRDPMHFIDLNHAVKRDPRTNMRSPNTNWDFWTSLPESLLQVTIIMGDRGIP
                     SSYRHMHGFGSHTYSFINDENKRTWVKFHFRSQQGIKNLTDQEAATVVGMDRESHQRD
                     LYEAIEKGVFPKWKMYIQVMTEEEANAMENNPFDLTKMWLKKDYPLIPVGEFELNKNP
                     DNYFAQIEQAAFNPANVVPGISFSPDRMLQGRLFSYGDAQRYRLGVNHHQIPVNAPKA
                     VENPHSFHRDGQMRVDANLGSELHYEPNSYGNWKDSPKDNEPSQAGGDVFRYDFREDD
                     SDYYTQPGLLFRAMTQDQKQVLFENTARNMGDSTLQIKHRHIYNCYQADSEYGKGVAR
                     AMNISLEEVDLDLPRKDSRENLQKANNMHPELNVPTEPVDSAREIDTNTKDYIKPEND
                     PWLL"
     gene            complement(2690..>3205)
                     /locus_tag="VFD08_02500"
     CDS             complement(2690..>3205)
                     /locus_tag="VFD08_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZK09917.1"
                     /translation="RMRYTYYASQAKKEGYVQMSNIFLETAENEKEHAKRFFKFLSAT
                     LEGESLEIKAEFPVALGDTKSNLLAAAEGEREEWTELYPKFADIADEEGFPQIAYVWR
                     EIAEAEERHELRYRKLLENIENNKVFEKDQEVEWKCNNCGYVHKGKAAPEVCPACDHA
                     QGHFEVFYETY"
BASE COUNT          859 a          691 c          576 g         1079 t
ORIGIN      
        1 aaatctttga tgactacgaa aagcttcttg gtctggatac ctttaaaaaa ctctttcctg
       61 tcttactaac agacagagga agtgaattta ctgatcccac tgctgtggag ttctctccat
      121 ttacgggaga gcgtagaagc tatgttttct actgtgaccc acagagagcc gatcaaaagg
      181 gagccataga aaactgccac tctctagcta ggagaatgat tcctaagtcg gaggacctta
      241 gaccctggac ccaggaaata ataaacgagg taggtgatca cctcaattcc tatccaagaa
      301 aaaagctgat ggataagtca ccatatgact ccttcagctt ttttctcggg gataacattt
      361 ccaacttatt acattggaaa aagattccct ctaaagacat tcaactaaac ccccattact
      421 ttagaaataa attaaagtaa gcaaaactat ggccaaaaaa gcacacaatc taaccttttt
      481 taggggtccc atttacctta gtaaatccca ccttactaag gggactcctc tttgtctcgc
      541 ccaaaattgg cagataaaga gaagctttgt cttataatac cataaaaaca aggaaatagt
      601 acttttacaa agacatcttc ccatctcttt taccttgata atacagatgg ctcacaaaac
      661 aaagattatt tcaactaggt gttgcattta ctttgacaac tgacctgatt attaagctta
      721 taaaaatagg cacagaaatc tgtgcctatg tcttatagga gccatggatc attttcaggc
      781 ttaatataat cttttgtatt tgtatctatt tctctagcag aatcaactgg ctcggtgggt
      841 acatttagtt ctgggtgcat attgttggcc ttttgtaggt tttcacggga atctttccta
      901 ggtaaatcca aatctacctc ttctagtgaa atattcattg ccctggccac acctttacca
      961 tattcagaat ctgcctggta gcaattataa atgtgtctat gctttatctg tagggttgag
     1021 tcccccatat tgcgagcggt gttctcaaaa agaacttgct tttgatcttg ggtcattgcc
     1081 ctaaaaagaa gacctggctg ggtataataa tcactatcat cttctctaaa gtcgtatctg
     1141 aaaacatctc cccctgcttg gcttggttca ttgtccttag ggctgtcttt ccaatttcca
     1201 tagctattgg gttcatagtg taattcactg cctaagtttg catcgacgcg catttgtcca
     1261 tcgcggtgga aactatgagg gttttctacg gccttggggg cattgactgg aatctgatgg
     1321 tgattgactc ctaggcggta gcgctgtgca tctccatagg aaaacagtcg gccttgtagc
     1381 atacggtctg gagaaaaact tataccagga actacattgg caggattaaa agcagcctgc
     1441 tcaatttgag caaaatagtt gtctgggttt ttatttaatt caaattctcc tactggaatc
     1501 agaggataat ccttttttaa ccacattttt gttaaatcaa agggattgtt ctccatagca
     1561 tttgcttctt cttctgtcat aacttggata tacatcttcc attttgggaa aacacccttt
     1621 tcaatagctt catataaatc tctttgatgg gactccctgt ccattccaac aaccgttgcc
     1681 gcttcttggt ccgttaggtt ttttataccc tgctgggacc taaagtggaa tttaacccag
     1741 gttcttttat tttcatcatt aataaaacta taggtgtgag agccaaatcc atgcatatgc
     1801 ctataggaag agggaatgcc tctatcaccc ataataatag tcacttgcaa aagtgattct
     1861 ggtagggagg tccagaagtc ccaatttgta tttgggctcc tcatattggt tcttgggtct
     1921 cttttaactg catgatttaa gtctataaag tgcatgggat ctctaaagaa aaataccggt
     1981 gtattgtttc caaccaggtc ccagttgcct tcttctgtat agaacttcat agcaaagcca
     2041 cgaatatctc tctcagcatc ggcagcgcct ctttcaccag caacggtaga aaaacgtgct
     2101 aacatctgcg ttgttttgcc cacttcagaa aagatacttg ccctggtgta ttttgtaata
     2161 tcatgggtta cagtaaaggt gccaaatgct cctgagccct tggcatgcat tctacgctct
     2221 ggtataactt ccctatcaaa atgagccatc ttttctaaaa accatacatc ctctaaggtc
     2281 attggccctc tcttgcccgc cgtcatggaa tcatgattgt ctgttaccgg agctcctgca
     2341 gctgttgtga tataagggtt ttctcctgta ttttgatttc tactaggagt ggactcactt
     2401 aatccattct tatccatagg gttgttagaa gtttttttct tgtcttttgt tttattatcc
     2461 ttcattttac tgtcctttct aggtgcctac aatgaaaatt gttgattcac tttatatttt
     2521 gcgtcttata atatataccc gagctataaa tctataaact tttagctttt tcaaagtatg
     2581 gacagccata ctttttgcta tagatttttt ccacctttac aagaaaaaac agagtgagaa
     2641 aaaccactct gttttatcct attatttaat aggctgtcca ttttataaat tagtaggtct
     2701 cgtagaatac ttcaaaatgt ccctgggcat gatcacaggc tgggcaaact tctggtgctg
     2761 ccttgccctt atgtacataa ccacagttat tgcatttcca ctcaacttct tggtcttttt
     2821 caaatacctt attgttttct atgttttcta atagctttct atatctgagt tcatggcgct
     2881 cttctgcctc tgcaatctct ctccatacat aggctatctg aggaaaacct tcttcatcag
     2941 ctatatctgc aaattttgga tatagttctg tccactcttc tctttccccc tcagctgctg
     3001 ctaataggtt ggattttgta tctcctaggg ctacaggaaa ctctgccttt atctctaggc
     3061 tctcaccttc tagggtggca cttaagaatt tgaaaaatct tttggcatgc tctttttcgt
     3121 tttcagctgt ttccaaaaag atattggaca tctgtacata accttctttt tttgcctgag
     3181 atgcatagta ggtatatctc attct
//
LOCUS       DASRNA010000091         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1072454,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000091 DASRNA010000000
VERSION     DASRNA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072454"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFD08_02505"
     CDS             complement(<1..1043)
                     /locus_tag="VFD08_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HZK09918.1"
                     /translation="MNKNNFRELRKKLDWPLMILPLLAVSVLTYLFAVDPVNSTRILD
                     TIRGFLGNELGFFYILIGLGILLTTLYIAFSDYGKIRLGKKDEKPQYSNYKWATMIFT
                     GVFAADIIFYSFVEWALYAGEPRIEQLGGVQEWASTYALFHWGPIPWAFYVMLAVAFG
                     FMLHIRGTKKQKFSEACRPLLGDGVDRLPGKVIDFIAIAALIAGTATTFSLATPLLAT
                     ALSKVFSLPMSTLLTVVVLAIIATIYTISVLIGMDGIIKSASICTSIFMGMLLFFLIF
                     GGEARYIVETGISSIGNLAQNFIGLSTWMDPLRTSGDGIDGFVQNWTIFYWAYWMAWC
                     VATPFFIGMISRG"
     gene            complement(1044..2825)
                     /locus_tag="VFD08_02510"
     CDS             complement(1044..2825)
                     /locus_tag="VFD08_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZK09919.1"
                     /translation="MNKRDWVILATIADLGYKNHRFIADRTDYSIGLVNTSLRKLIDQ
                     DYLDKDYTITNKTKKYMKKSKPQRAVILAAGTGIRMMPISKTPKCLLEINNEALIERI
                     IKQLNEVGVFEIYVVVGYQIEQVEFLSNKFDVELIYDELFARRDNLHSLSLASDHLSN
                     AYVVPGNVWFARNPFSINEYFSWYAISPYLDDESYLRLNRKMELVYIEDETPGNSMIG
                     LAYLLDKDAQLVKKELHMLSNKRKYNRETWERSLFVDNKLLPYARVMLGQSAYKINTY
                     EDLRELDSYSKDLLSNHINLISQVFKVPSKEITHISPLEKGMTNKLMRFSIGDEPYLL
                     RIPGVGSNELTIRQQEADVYQALRGKKLTDEIIYISGQSGYKISKYWENSRTCDAQSQ
                     EDVSMCMKHLKKLHDMKLEVIHSFNPLKRLDIYENLLTSPPAFADYDVTREKVCELIG
                     YLDKLPKEECLCHIDSVTDNFLIFDDQPYLIDWEYAGMCDPPIDIAMFSIYANYSKEE
                     IDQLMHTYYGEEPTELNRFKVYAYAAIGGLLWSVWCEYKNQFGATFGDYAVKQYRYAR
                     DFHKYAMKVLNTQEVIGILRREGRLDA"
     gene            complement(3050..3814)
                     /locus_tag="VFD08_02515"
     CDS             complement(3050..3814)
                     /locus_tag="VFD08_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020755091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZK09920.1"
                     /translation="MKLKDKVAIVTGASGGMGRDIAYLFAKEGATVYAAARRVERLEE
                     LAESSKELAGKILPYAVDLMNQEDAEKMIDFVKEDAGKLDILVNNAGLMDDFSPVGDV
                     TDKMLERVFNLNVFSLFYTMRKAVQIFLEQGEGNIVNVASIGGLFGARAGAVYTASKH
                     AVVGLTKNTGFMYAKNNIRCNAICPGGVDTEIGSSEFMKDINEFGMGAIMPMTVGNPR
                     SGTGEEIATIALFLASDDSSFINGQAIAADSGWTAY"
BASE COUNT         1215 a          811 c          767 g         1181 t
ORIGIN      
        1 cttcccctac taatcatacc aatgaaaaat ggggttgcca cacaccaggc catccaatag
       61 gcccagtaaa aaatggtcca attttggaca aaaccatcaa taccatctcc cgatgtccgt
      121 agggggtcca tccaagtgga taggccaata aagttttggg ccaaatttcc tatggaagaa
      181 atccctgttt caacaatata gcgggcttct ccaccaaaaa taagaaaaaa cagcagcatc
      241 cccataaaga tactggtaca tatggaggcg gatttgataa tcccatccat tccaatcagg
      301 acagaaatag tgtaaatagt ggcaataata gcaagaacca ccactgtaag tagggtggac
      361 ataggtaggg aaaaaacttt gctaagtgct gtagcaagca ggggggtagc cagggaaaag
      421 gtagtagcgg taccagctat tagggctgct atggcaataa agtctataac ttttcccggc
      481 agcctatcaa ccccatcacc caggaggggc ctgcaagctt cagaaaactt ttgttttttt
      541 gtccctctaa tatggagcat aaaaccaaag gccactgcca gcatgacata aaaggcccag
      601 ggtatgggtc cccagtggaa aagagcatag gttgatgccc attcttggac cccgcccagt
      661 tgttcaatgc ggggttcacc agcataaaga gcccattcta caaaggagta aaatataata
      721 tctgctgcaa agacaccagt aaaaatcatt gtggcccatt tataattgga atattgcggt
      781 ttttcatctt ttttacccag gcgtatcttg ccatagtcag aaaaagcaat atacaaggtg
      841 gtaagtagaa tgccaagacc aattaggata tagaaaaaac ctagttcatt accaagaaaa
      901 ccacgaatgg tgtctaggat tcttgttgaa tttaccggat caacggcaaa tagataggtt
      961 aagacagata ctgccaaaag aggcaggatc attaggggcc aatcaagttt ctttcttaat
     1021 tctctgaaat tatttttatt catttatgca tccaatctgc cttctcttct taaaatacct
     1081 attacttctt gtgtattaag gaccttcata gcgtatttgt gaaaatcacg agcgtagcgg
     1141 tattgtttga cagcataatc accgaaagtg gcgccaaact gatttttata ttcacaccag
     1201 acagaccata aaagaccacc aatggcagca taggcataaa ctttaaatcg attcaattct
     1261 gtgggttctt ctccatagta ggtatgcatt aactgatcaa tttcttcttt tgaataatta
     1321 gcatagattg aaaacatagc aatatcgatg gggggatcac acatgccagc gtattcccag
     1381 tcgataagat agggctgatc atcaaaaatt aagaagttat cagtaacaga gtcaatatga
     1441 cagaggcatt cttccttggg cagcttgtct agatagccta ttaattcaca gaccttttct
     1501 cttgtcacat cataatcagc aaaggctggg gggcttgtta gtaggttttc atatatatct
     1561 agtcttttta aggggttgaa actatgaatg acttctagtt tcatatcgtg tagttttttt
     1621 aaatgtttca tacacataga aacatcctct tggctttgag catcacaggt tctggagttt
     1681 tcccaatatt ttgaaatctt ataaccgctt tgaccagaaa tgtatataat ttcatccgtg
     1741 agctttttcc ccctaagggc ttggtataca tcggcttctt gttggcgaat ggtaagttca
     1801 ttgctgccaa caccaggaat ccgtagaaga taaggctcat caccaataga aaagcgcata
     1861 agtttatttg tcatgccctt ttctagaggg gagatatgag taatttcttt ggatggtacc
     1921 ttaaagactt gactgattag gtttatatga tttgagagaa gatccttaga ataactgtct
     1981 aattcccgca agtcttcata ggtgttaatt ttataagcgg attggcccag cataaccctg
     2041 gcatagggga ggagtttatt gtccacaaaa agagagcgct cccaagtttc tctattgtat
     2101 ttacgtttat tgctgagcat atgtaactct ttttttacca gctgggcatc cttgtctaaa
     2161 agataagcaa gcccaatcat ggagtttcct ggagtttcat cttctatata gacaagttcc
     2221 atcttcctat ttaagcgcaa atagctttca tcatctaaat agggtgagat ggcgtaccaa
     2281 gaaaagtatt cattaatgga aaaaggattt ctagcaaacc acacattgcc aggaacaaca
     2341 taggcattag aaaggtggtc cgaggccagg ctaagggagt gaagattgtc ccttctggca
     2401 aagagttcgt cataaataag ttcaacatca aatttatttg aaagaaattc cacttgctct
     2461 atctggtagc ccactacaac atatatttca aaaactccta cctcattgag ttgttttata
     2521 attctctcaa taagcgcctc attgtttatt tccagtaaac atttgggggt cttgcttatt
     2581 ggcatcatac gaatgcccgt gccagcggca aggataacgg cgcgctgggg cttggacttt
     2641 ttcatgtatt tttttgtttt attggtaatt gtataatctt tatcaaggta atcttgatca
     2701 attagctttc taagtgaagt gtttacaagt cctatagaat aatctgttct atctgcgata
     2761 aagcggtggt ttttatatcc aagatcagca atagttgcta ggatcaccca atctctcttg
     2821 ttcatcataa gctcctttgt gtatatcatg aaaacatcac gcgcaataaa tataacatat
     2881 atacaaaatc gtgtcaaatt aaagcgcggt gggttcaaat aagccgtaaa tttgccttta
     2941 gcgaaaaata caagcgggtt attttctttg ctttattatc taaagaaaaa aagcgctgga
     3001 atctcattac tagagatctc agcgccttaa tgtgaaagct ttttaacttt tagtaggctg
     3061 tccagccact atcagcagcg atggcttgcc cattgataaa gctagaatca tctgatgcta
     3121 ggaaaagggc aatggtggca atttcttcgc cagttccact tcgaggattt ccaacagtca
     3181 tgggcataat ggctcccatg ccgaattcgt tgatatcctt cataaactca ctactgccaa
     3241 tttctgtatc taccccacca ggacaaatag cattgcaacg gatattgttt tttgcataca
     3301 taaaaccagt gttctttgta aggcctacta cagcatgctt agaggcagta tatactgctc
     3361 cagctcttgc accaaatagt ccacctatgg aagcgacatt gacaatatta ccctcgcctt
     3421 gttcaaggaa aatttgcaca gcctttctca ttgtataaaa tagggagaaa acattgagat
     3481 taaagactct ttcaagcatt ttatcggtaa catcacctac gggactaaag tcatccataa
     3541 gcccagcatt gttaacaaga atatctagtt tcccggcatc ttcttttaca aaatcaatca
     3601 tcttctcagc gtcttcttga ttcattagat caacagcata gggaaggatt ttgcctgcta
     3661 gctcttttga gctttctgct agttcctcca gacgttctac tcggcgagct gcggcataaa
     3721 cagtggcccc ttcttttgca aaaagatagg cgatatctct gcccatacca ccacttgcgc
     3781 ctgtaacaat agcaacctta tcttttagtt tcattttacc tcccttttga cttacagtga
     3841 aatattggtc tttttagtat acatcatcgc tagggggaaa gaaagaaaaa tatctcaata
     3901 aattaaaaaa gaacgccgtt cgttaaacga taaagacatg tatattgtaa taaagacaga
     3961 gggtatttcc actt
//
LOCUS       DASRNA010000092         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_926526,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000092 DASRNA010000000
VERSION     DASRNA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926526"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFD08_02520"
     CDS             <1..690
                     /locus_tag="VFD08_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZK09921.1"
                     /translation="VITISTVGYAEVALMDNEAKIFSIFLIFVSLGTVGYLFSSIVSS
                     LLEGDLRLAWRKKRMEKDIVRLKDHYIICGAGETGINAIKQFQKSDIDFIVIEKNEEK
                     IEALVEDDIFVIQGDSTDEAILEKARITFAKGLISTLPNDADNVYTVLTARSKNSELY
                     IVSRAIEKNADQKLKRAGANNTISPNEIGGNRMAALILRPTVISFLDIMTHVGEVVLD
                     LENVTVEKNHQ"
     gene            complement(1000..1656)
                     /locus_tag="VFD08_02525"
     CDS             complement(1000..1656)
                     /locus_tag="VFD08_02525"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZK09922.1"
                     /translation="MKIIDLTHSIAPDMPVYPGTEPPRFELGNTYEKDGFKETLISFF
                     THTGTHIDPPAHLYKEGKTLDDYPVSQFAGQALVINVRHLKEGQAISMEDLKRYGKKI
                     DEAEFILFNLGWDKRWGSKEYFADYPCIDEEVLAWLIAQAKAGKKKGLGFDVIGLDPI
                     ADSELTRHKKLFKECELINIENLCNLDQCGDQLFMFTWLPIKLKKSDGAPCRAIAYIK
                     "
BASE COUNT          668 a          338 c          385 g          630 t
ORIGIN      
        1 gttataacca tatccactgt aggatatgct gaagtagcac tgatggataa tgaggcaaaa
       61 atattttcta tatttcttat atttgtaagc cttggtactg taggttattt attttcaagt
      121 attgtaagtt cacttttaga aggagactta agacttgcct ggaggaaaaa acgtatggaa
      181 aaagatattg tgaggttaaa agaccattac attatatgtg gcgctggaga aacgggtata
      241 aatgcaatca agcaatttca gaaatcagat atagatttta ttgtaattga aaaaaacgaa
      301 gaaaagatag aggccttagt agaggatgat atatttgtga ttcaaggaga ctcaactgat
      361 gaggctatac ttgaaaaggc tagaattact tttgcaaaag ggcttatatc tacccttcca
      421 aatgatgcag acaatgtata tactgtttta accgccaggt ctaaaaatag tgagttatat
      481 atagtctcta gagccataga aaaaaatgca gatcaaaaac taaagcgggc aggagccaat
      541 aataccatat cccccaatga aattggagga aatagaatgg ctgctcttat acttagaccc
      601 actgttatat cctttttaga tataatgacc catgtagggg aagttgttct tgatttagaa
      661 aatgttacgg tcgaaaaaaa tcatcaataa aagaaaaaac attaagagaa gcaaaaatac
      721 ctgaaaaaac aggccttatc atccttgcta ttagaaaaaa agagggcgac aattggattt
      781 ttaatccaaa ttcagatgaa ctcctagaat taggggatat tatggtggtt ttaggaacag
      841 aagaacaagt agagatgctg aaaaaactat aatgctcgtt attactaggg ggcatataat
      901 tcaagcaatt tataaactat ccccccttaa aacattttag ggagtcctta tattgtaagg
      961 gcttttttta ttcttattga gtgagtttaa tacaagttat tattttatat aggcgattgc
     1021 cctgcaagga gcaccatcgc tttttttcag cttaattggc agccaagtga acataaatag
     1081 ctggtctccg cattggtcga gattacataa attctcaatg tttattagtt cacattcttt
     1141 aaaaagcttt ttatgtcttg taagttcaga gtctgcaata ggatcaagtc ctattacatc
     1201 aaagcctagc cctttttttt tgccagcctt ggcttgggcg attagccagg caagaacttc
     1261 ctcatcgata caggggtaat cagcgaagta ctccttgctt ccccatcttt tatcccatcc
     1321 taaattgaac aaaataaact cagcctcatc aatctttttg ccatatctct tcaagtcctc
     1381 catgcttata gcctggccct ccttaagatg cctgacattg attacaagag cttggcctgc
     1441 aaactgggat acaggataat catcaagggt tttaccctcc ttataaagat gagctggagg
     1501 atctatatgg gtgcctgtat gagtgaaaaa agagataagg gtttctttaa agccatcttt
     1561 ttcataggta ttaccaagct caaacctggg tggctctgta cctgggtata caggcatatc
     1621 tggagcaatg ctgtgagtta aatcaattat tttcatttct ttttcctttc tgaaaaaaag
     1681 ctatataaca gtctaatctt ttttcgaatc caatccctat agaggctata tttatagtaa
     1741 agcccatggg aaattatttt gcccatcaag tggaggactt gcaagtcaat ggaaagtatt
     1801 tactaaaggc acctataagt atacagcctc tcctgcatct ggtctattat tcttgggtag
     1861 ttgggggcgt aatcaaagtc atagggagct aattcttgct aaagtcaaag gcagaaagat
     1921 tattagattg actttacctg tataaataaa aagtctgata ttttaaccga aaccttattt
     1981 atcactagat atgatgagac tttagttatg aatatgtagt a
//
LOCUS       DASRNA010000093         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_29102,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000093 DASRNA010000000
VERSION     DASRNA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29102"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFD08_02530"
     CDS             complement(<1..513)
                     /locus_tag="VFD08_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1727 domain-containing protein"
                     /protein_id="HZK09923.1"
                     /translation="MRFLFTLWLARATVFAMKLMGKRGSYLPGKLAIQLYPDFLSGFN
                     PPKEIIGITGTNGKTTLTNLLGDFFKSQNLEYFSNSYGSNTVEGIAACYIHNSNFFGK
                     LYYERAVLEIDERSALKIFKHVHPDWLVVTNLFRDSFPRNAHSDYIFDILEEAIPTKT
                     RLILNSDDLIS"
     gene            complement(517..1536)
                     /locus_tag="VFD08_02535"
     CDS             complement(517..1536)
                     /locus_tag="VFD08_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HZK09924.1"
                     /translation="MKISRLFQIQWKNVFNLSRELAKRSGRNPMAVFLDFLWSYKTRG
                     ASWWNYMNFGFHFQKDPEVRDSFATEYKDNIYMNRYCNSPEMLRTLMDKGEFNKHYKE
                     FLGRDYLDLRLASEEEFHLFLKRHGEVVIKPAFDMGGSGIEKLSAQEAKGIYTKLKEE
                     KKFLVEEVLKQHPEMSSINASSINTLRTCTCIDKEGKITILYMVLRIGRKDSFIDNMS
                     AGGLFTKITMDGQITHPCYSAQGFGSIYTHHPDTKLPFIGIQVPMIKEVKNFAIKLAE
                     KNPDARYVGWDIAITPQGPVVIEGNDRPSCDLPQTYIHLDSGRGLVEEYERALSIKLP
                     RSRKG"
     gene            complement(1547..>2096)
                     /locus_tag="VFD08_02540"
     CDS             complement(1547..>2096)
                     /locus_tag="VFD08_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HZK09925.1"
                     /translation="VYAWLYENEITLLEETVIQHPVLEKVSPGCVATVRITTVVTPEE
                     DVLFFSPLLRMGEGEVGHIISGQFYAPINEEGVIFAPAFKQDGDYDQRGGDFIYHHPS
                     TGEELVGFSVPHFKKAEEMCRQMALEIKSYPFIGWDIGITEEGPVLLEGNKFPANDII
                     QVYKYHPQQRGLYYEIKNALGL"
BASE COUNT          633 a          451 c          358 g          654 t
ORIGIN      
        1 acttattaga tcatcactat taagaataag cctggtcttt gtaggaattg cttcctcaag
       61 tatatcaaag atataatcag aatgggcgtt gcgggggaaa gagtctctaa agagattagt
      121 cactactaac caatcaggat gaacatgttt aaaaatttta agggcactac gttcatcaat
      181 ctcaagaact gctcgctcat agtacagttt gccaaaaaag ttactattgt gaatgtagca
      241 agctgcaata ccctccactg tattagagcc ataggaatta gaaaagtact ctaaattctg
      301 ggacttgaaa aaatccccta gtaaattagt gagagtggtt tttccgttgg taccagtaat
      361 tccaataatt tctttgggtg ggttaaaacc actcaaaaaa tctggataaa gttgtattgc
      421 tagtttaccg ggaagataac ttcctctttt tcccatcaat ttcatagcaa aaacagtagc
      481 cctagcaagc cataaggtaa ataaaaaccg catattttat ccttttctac ttcttggtag
      541 ttttatagag agggcccgct catattcctc tacgagtcca cgaccggaat ctagatgaat
      601 ataggtttgg ggaagatcac aacttggtcg atcatttccc tctatcacaa ctggcccctg
      661 aggggtaatt gcaatgtccc aacccacata acgtgcatcc ggattttttt ctgccaattt
      721 tatggcaaaa tttttgactt cttttatcat tggaacttgg attcctataa aagggagctt
      781 tgtatctgga tggtgggtat aaatactgcc aaatccctgg gcactataac agggatgcgt
      841 aatctgtcca tccatggtta tttttgtaaa caagccccca gcactcatat tatcaataaa
      901 ggaatctttt cttcctatgc gtaaaaccat ataaaggatg gtaattttac cctctttatc
      961 tatacaggta caggtcctca gagtatttat agagctagcg ttaatagaac tcatttcagg
     1021 atgctgttta agaacttctt ccacaagaaa ttttttttct tcttttagct ttgtgtaaat
     1081 acctttagcc tcttgtgcag aaagcttttc aatacctgag cctcccatat caaaagctgg
     1141 ctttataaca acctctccat gacgctttaa aaacaagtga aattcttctt cactggccaa
     1201 acgaagatct agataatctc tcccaagaaa ttccttgtaa tgcttattaa attccccctt
     1261 atccataaga gtacggagca tttcagggct attacaatat ctattcatat agatattatc
     1321 tttgtattct gtagcaaaac tatctctcac ctcgggatct ttttgaaaat gaaaaccaaa
     1381 gttcatatag ttccaccaac ttgctccccg tgttttatag ctccataaaa aatccaagaa
     1441 aactgccata gggttacggc cactcctctt agctaactct ctagataaat taaatacatt
     1501 cttccactgg atttgaaaaa gacgagatat tttcatattt tagcctttat aatccaaggg
     1561 catttttaat ttcataatat agcccgcgct gctgtggatg atatttgtag acttgaataa
     1621 tatcattggc gggaaattta tttccctcta aaagaacagg accttcctcg gtaatgccaa
     1681 tatcccaacc aataaaagga tagctcttga tttctagtgc catctgtctg cacatttctt
     1741 cagccttttt aaaatgggga acgctaaatc ctactagttc ctcccccgtc gaaggatgat
     1801 gataaataaa atcccccccc ctctggtcat aatctccgtc ttgtttgaag gcaggggcaa
     1861 aaattacgcc ctcctcattt ataggtgcat aaaactggcc agagattata tggcccactt
     1921 caccctcccc cattcttaga aggggggaaa agaaaagcac atcttcctct ggagtaacaa
     1981 ctgtcgtaat tctgactgtt gcaacacatc cagggcttac tttttccaaa acagggtgct
     2041 ggattaccgt ttcttccaga agggtaatct cgttttcata aagccaagca tatact
//
LOCUS       DASRNA010000094         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_691728,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000094 DASRNA010000000
VERSION     DASRNA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691728"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFD08_02545"
     CDS             <1..902
                     /locus_tag="VFD08_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK09926.1"
                     /translation="IMYVGTLLTTVRRIVEFTEQFQRGMTGIERFNEIMDTDIDIFDE
                     EGAREILHPRGDLEFKNVNFGYEEENGRVLKNINIKINSGENVALVGPSGGGKTTLCH
                     LIPRFYEVEEGEILLDGTNIKDFTIKSLRQAVGLVQQDVYLFGGSVYDNILYGNPQAS
                     YEEVEEAARLAGAYDFISELPQSFDTYVGERGVKLSGGQKQRISIARVFLKNPPILIL
                     DEATSALDNTSERLVQKSLERLSQGRTTLTIAHRLSTIQNADRILVLDKGEIIEEGTH
                     RELLSKKAAYYKLHQLSFPEEAI"
     gene            complement(915..1835)
                     /locus_tag="VFD08_02550"
     CDS             complement(915..1835)
                     /locus_tag="VFD08_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZK09927.1"
                     /translation="MYYSISEKNTLSSPPTKGEAFLAYLSLDQLIKDRSELGVSREVL
                     EISREENPHNILLAQEDYYYIILLLPNLKESNLSGHPLGIFLDKGKLLLINLEEEELS
                     KEFFQGALSKQAKLSSPSHFLFSFLREILHSHYEKYLAYQAIIQNFEEKIWITESKNK
                     KLEHDLSSLSIKLLVLQNFYDQLSELLDQLEEYESDILPEKELPLLLSLHSRVDHYAN
                     NIHFLKDYLVSLRSTYQAQLTLSMNAVMKIFTVISAVFLPLTLFVGWYGMNFKYMPEL
                     GWRYGYLGFGAFILLTSGLILFYLRKRKLL"
     gene            1956..2804
                     /locus_tag="VFD08_02555"
     CDS             1956..2804
                     /locus_tag="VFD08_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK09928.1"
                     /translation="MSEISKGKVYIVLSAIIFGLMPLGASLAYRGGSNALQLVFFRLS
                     VVLPLYWFLARKKGQSLKLTRKQAVQIQFLSLGFTFTPISLYLSYYYIDSGVATTLHF
                     MYPLIVVMACWIFYAEKPGLKMWLCLLLTLIGLFMNMPRESAGILGIVLALISALTYA
                     FYVVYLAKSGLRELGSMALLFHICLFGSLQTGLVTTLFKSWSPMNAFGVIASLVFALV
                     VSFGAMSFQKGTFIIGPQKASILSTLEPLTSILVGLLLLGDKISLISGSGLVLILLSS
                     FVLSIE"
BASE COUNT          815 a          587 c          756 g          910 t
ORIGIN      
        1 atattatgta tgtaggaacc ctgctaacaa cggttcggcg gattgtggaa tttaccgagc
       61 agttccaaag agggatgaca ggaattgagc gctttaatga aatcatggat accgatattg
      121 atatctttga tgaagaaggt gccagggaga ttctacatcc ccggggggac ttagaattta
      181 aaaatgtaaa ctttggctat gaagaagaaa acggcagggt tcttaaaaac ataaatatca
      241 agataaattc cggagaaaat gtggccctgg ttggccccag tggcgggggt aagaccaccc
      301 tttgccatct gattcctcgt ttttatgagg tggaggaggg tgaaattctt cttgatggca
      361 ccaatataaa ggacttcacc ataaagagcc tgcgccaggc cgtgggccta gtccagcagg
      421 acgtttatct ttttggtggt tctgtctatg ataatattct ttatggaaat ccccaggcta
      481 gctatgagga ggtggaggag gcagcccgtc ttgcaggcgc ctatgacttt attagcgagc
      541 ttccccagtc ctttgatacc tatgtagggg aacggggggt taagctctca gggggccaaa
      601 agcagcgtat tagtattgcc agggtttttc tgaaaaaccc acccattcta attttggatg
      661 aggccacttc ggccctggat aataccagtg aaagactggt gcaaaaaagc ctggagcgac
      721 tgagccaggg gcggacaacg ctgaccattg cccatagact tagtaccatc caaaatgccg
      781 accgcatcct ggtgctggat aagggtgaaa tcatagaaga aggaacccat agggagctcc
      841 taagtaaaaa agcagcttat tataagctgc accaactaag ttttcctgaa gaagccattt
      901 aagatggctt ctttttacag gagttttctt tttcttagat aaaagagaat gagaccgcta
      961 gtaagaagga taaaggctcc aaagcctagg taaccatagc gccaacccag ttccggcata
     1021 tatttaaagt tcattccata ccagccaaca aaaagagtta agggtagaaa tactgcgcta
     1081 attacggtaa agatcttcat aacagcattc atgcttaggg tgagctgggc ctggtaggta
     1141 gagcgaaggc tgaccagata gtcttttaga aagtggatgt tgttggcgta gtggtcaacc
     1201 cggctatgga ggctaagaag aagggggagc tccttttcgg gcaggatatc actttcatac
     1261 tcttctagct ggtctaggag ttctgagagc tggtcataga agttttgtag gaccaggagt
     1321 tttatactaa ggctgcttag gtcgtgttct agctttttgt ttttgctttc tgttatccag
     1381 attttttctt cgaaattttg aatgatggcc tgataggcca ggtatttttc atagtggcta
     1441 tggaggattt ctcgcaaaaa cgaaaagaga aaatggctgg ggctagaaag ctttgcctgc
     1501 ttactaaggg ccccttggaa aaactctttg gaaagctcct cttcttccag gtttataagc
     1561 agaagcttac ccttatcaag aaagattcct agtgggtgac ctgatagatt ggactctttt
     1621 agattgggaa ggaggaggat aatatagtaa tagtcctcct gggctagcag aatgttgtgg
     1681 gggttttcct ccctagaaat ctctaggacc tccctactta ccccaagctc tgagcggtct
     1741 tttatcagct ggtctaggct taaataagca aggaaagctt cccccttagt aggaggagaa
     1801 gaaagggtat ttttttctga aatagagtaa tacatagggg ctccttctat gctatagtag
     1861 agcaaagtgg catggttttt cttttatata atgattatac cccttgacta ggggggatag
     1921 ttaaaaaata ccataaatga taaagaggtc ggaaaatgag tgagatttca aagggaaaag
     1981 tatatattgt tttatcggct attatttttg gcctaatgcc cctaggggct agtttggctt
     2041 ataggggtgg ttccaatgcc ctgcaactgg tttttttcag gctcagtgta gtccttcccc
     2101 tttactggtt tttggcaaga aaaaagggcc aaagcctaaa acttacaaga aaacaggctg
     2161 tgcagatcca gtttcttagt ctgggcttta cctttacccc aatcagctta tatctgtcct
     2221 attattatat tgattctggg gtggctacca ccctacattt tatgtacccc ctgattgtgg
     2281 ttatggcctg ctggattttt tatgcggaga agcccggtct taagatgtgg ctttgtcttc
     2341 ttctaactct aattgggctt tttatgaata tgcccaggga aagtgcagga attctgggca
     2401 ttgtcctggc ccttatttct gctcttacct atgcttttta tgtggtttat ctagccaaaa
     2461 gcggcctaag ggagctgggt tctatggccc ttctttttca tatttgtcta tttggctccc
     2521 tgcaaacggg ccttgttacc actcttttta agtcttggag cccaatgaac gcttttggag
     2581 tcatagccag tctggtcttt gccctggtgg tgagttttgg ggccatgagt tttcaaaagg
     2641 ggacctttat tatagggccc caaaaggctt ctattttaag taccctagaa cccctgacca
     2701 gtattcttgt gggccttctt cttctaggag ataagattag ccttatttca ggcagtggcc
     2761 tggtcctgat tcttttgtcc tcctttgtcc ttagtataga gtagagaaat ttatctaaga
     2821 aagatcagtg agataagata agtagggagg gtagataaga agaaaggccc gctttttttc
     2881 tagggccctc ttgtgatata ataggcctga aaatcttttt ttaaaaaagg agtgtagaat
     2941 gaaaaggaaa atgtgtctta ttactttagt ggtatttgct ctgctagtga gcagctgctc
     3001 tggacctgag caaatagaaa ctagtgacac agtgcttcgc atggcaaagt ctgtggaccc
     3061 tgatggac
//
LOCUS       DASRNA010000095         9834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_176620,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000095 DASRNA010000000
VERSION     DASRNA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176620"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..607
                     /gene="rpsF"
                     /locus_tag="VFD08_02560"
     CDS             323..607
                     /gene="rpsF"
                     /locus_tag="VFD08_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZK09929.1"
                     /translation="MNKYESIVVFRPDLEEDARVKLLERFKEIIGRYGKVESVDDWGM
                     KRLAYEIEKLTDGYYYLINFEANSDLPEELERNYKIQDQVIRYNIIRKDA"
     gene            617..1030
                     /locus_tag="VFD08_02565"
     CDS             617..1030
                     /locus_tag="VFD08_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZK09930.1"
                     /translation="MNQVILIGRLTADPELRFAASTGRAVTNFRLAVDRPFSREKKTD
                     FFRVVVWGKQAEAVAKYLRKGSQCAVNGSIQIDQYTDREGNTRYSTDIVANQVEFLGS
                     PGGARKESSNDSDSYESPNDADTDFPLVDDEDIPF"
     gene            1039..1257
                     /gene="rpsR"
                     /locus_tag="VFD08_02570"
     CDS             1039..1257
                     /gene="rpsR"
                     /locus_tag="VFD08_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZK09931.1"
                     /translation="MAFRPRRKKVCYFCTNKIDDIDYKDVRTLKRYVSDHGKILPRRV
                     TGACAKHQRKVALQIKRARAVALLPYVD"
     gene            complement(1309..2277)
                     /locus_tag="VFD08_02575"
     CDS             complement(1309..2277)
                     /locus_tag="VFD08_02575"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HZK09932.1"
                     /translation="MIPLFDAHFDLLSEISFKRKQGKSHVFRDDFYPRFKKGGVKIVI
                     ASIYLQGHELNQPFYYAMEQLGFFYEELRESPELLMLIRTKEDLALCQASDKIGLLLS
                     FEGAEPLLKPRDLYVFYELGVRLLGLSWSRRNIYADGCDFVQGPSKKGGLSPAGHELM
                     ELAHKLGIIPDVSHLSDEGLEDIIRLDIPLIASHSNANSITPTPRSLKDKYLDILRKK
                     DFGLGVNGAHFIISRDEKTSSIEDFIAHMDYLIKKLGQDKVGLGLDLCSCLSVFKGDE
                     IIKPEVIAHHGQAQQLLGALEDKFGPEITQKIAFDNWYNFFSRHLP"
     gene            2495..3946
                     /locus_tag="VFD08_02580"
     CDS             2495..3946
                     /locus_tag="VFD08_02580"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZK09933.1"
                     /translation="MRKKDNYSMLMDLYEMTMSMGYFESGFKDTIVYFDMFFRRTPDK
                     GGFAIAAGLAQLIEFLKNLHFTEEDISYLRGLGIDNEDFLDYVRKFKFTGDIWAVEEG
                     TPVFPGEPLVTVRAPVIEAQLIETALLVIINHQTLIATKANRIVRAAQGKSIIEFGAR
                     RAQGRDGAYMGARAAYIGGCIGTSNLMAGRDFDIPVYGTMAHSWIQLFDDEEEAFRAY
                     AKTYPQASSFLVDTYNTLKTGVPNAIKVFDEVVVPSGHRPSSIRLDSGDLAYLSKEAR
                     KMLDQAGYPDVKIMASNSIGEHTLQDLRDQDAEIDLYGVGERLITASSDAVFGGVYKL
                     VALEDKGVIEPKIKISENVEKITTPGFKELYRIYDGVSEKALADLITLRGEEIDPSQP
                     YTLFDPVHTWKKTRVENFILKKLLVPVFKGGKCVYPEKSIQEIRAYREKEEDKLWNGV
                     KRIKNPHKYFVDLSQELWDTKQDLLVRYAKRGR"
     gene            3946..4842
                     /locus_tag="VFD08_02585"
     CDS             3946..4842
                     /locus_tag="VFD08_02585"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HZK09934.1"
                     /translation="MKKSKSNLLLGLLGLSVIVGIFSGFIPTIFVYSLSLLFAYVSTR
                     YGDVGYVLPGLAMMLLGGLFLYGPSISLVEIGLLMLYGVFMGMSFSKRLRPEMIYFPT
                     LAILFAIMLLTFFLQKSLLGVDGLEAVIDTYMNQVQASVNDPQLLINLRATIKEFSLS
                     IVFLVAVMLNLFLLWIFSKILDIRKLKNTGRFHFEFFRLRELKFFQIIQLIGLVILGS
                     LLTGMPTQTALGNLVFILIAIFYLQGLSLSIFLLKSRKRGRILTALVVLLSLTVPFIQ
                     LFLLILGLIEQSKDFRKLEKVL"
     gene            4839..6815
                     /locus_tag="VFD08_02590"
     CDS             4839..6815
                     /locus_tag="VFD08_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZK09935.1"
                     /translation="MKDKKKITELYRRNTWIYLFFLALLIAFIFIGNRDLGYVALGLY
                     LIIFSYTLYFQLRSRNQWETMLEDLRLRVEEGNISSYLNMPLPILLVDETGMIIWHNS
                     KFKNIFDSETGFIGQKLETIFPLLNWNQVMEGPYFFNQALGDKQFRVFRSSTRNKGVN
                     IYTLYWYDETDFAKLKQRYKDERPLAVYIQLDNFDEVVKETDESRLPFLLSGINSVIR
                     KWALDHQGIIHKINEDEYLVLVENSFLASMESRKFTIIDDVRKIEEGNRYPMTLSVGV
                     SADGASFSERDEGSQQALELALGRGGDQVVVRKDDNYEFFGGRSKNVERKSRVKSRVV
                     AQGLAGLIKESERVYIMGHNYGDMDSFASTIGMYRACLNLDVPAYIVLNKSTDAIEEL
                     YALFRDNKLYRFISGAQAAGARNHDKDLLIVVDTHRPNFTEAPELLPLFERIVVIDHH
                     RRGAEMIENTSLLYQEPYASSAAEMISEILQYITSDVVLEPLEASALLSGIVLDTKNF
                     VFNTGVRTFDAASFLRRHGADTRLVRGLFEEDLLESVIKSNIVSSALLIEEGIALSQS
                     SENSANIKTIISQGADELIDIKGIHTSFVLGCDLEGKVYISARSLGDTNVQVILEKLG
                     GGGHLETAGAQLEDTTIEEAKTLLLETIKESLEE"
     gene            6816..7262
                     /gene="rplI"
                     /locus_tag="VFD08_02595"
     CDS             6816..7262
                     /gene="rplI"
                     /locus_tag="VFD08_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZK09936.1"
                     /translation="MKIILLEDVKDMGKKGDMVEAKAGYARNYLIPRGLAAEATQSNV
                     TELKHQRHAKSKKAAKALARAQELKGKMEEEVLKMKVKAGEGGRIFGSVTTMDISAGL
                     KELGYTVDKKRVVLDQPLKTLGEHKVKLKLHHEVEAEIRLLLVGEE"
     gene            7262..8569
                     /gene="dnaB"
                     /locus_tag="VFD08_02600"
     CDS             7262..8569
                     /gene="dnaB"
                     /locus_tag="VFD08_02600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZK09937.1"
                     /translation="MNLPHSIDVEKALLGSMLQNKKAMSIGIEQLEAYDFYDEANGLI
                     FSAIVSLADKSDSLDITMLRDYLENKGTLLRVGGVEYLIHLTEAGLRPYNALSYIQSI
                     KEKSVRRKLIEAASEITKISKDTTQDLDVLGEAERRIFNLADESTQQGLVHVASELTD
                     MMDHLEEASANPEGMRGLSTGFIELDLKTNGLHKGDLVLIAARPSMGKSALAMNIAQH
                     AALHENKNVCVFSLEMGKSSLLYRVLASEYLISLTNILTAKLSGEDWKKLSRGVGALS
                     NAPLYIDDTSGISVMQIRSKLRRHRLEHGEIDLVVVDYLQLMDGGGGENRQVEIAGIS
                     RGLKALAKEFNCPVLALSQLSRGPEQRSDKRPLLSDLRESGAIEQDADIVMFIYREDY
                     YNKDTELQNISEIIISKQRNGETGTVQLAWLGQHTKFADLHRG"
     gene            8671..9264
                     /locus_tag="VFD08_02605"
     CDS             8671..9264
                     /locus_tag="VFD08_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HZK09938.1"
                     /translation="MIEKADWSNKGVKITLDGEEYLIHTKAYMEANLSEGQEVDLDAL
                     LLRSAYFYGLEKAESYLLRGLRTRKQLRDYLIAREFIDPVENILNFLEEYGLINDYDY
                     AKTYYEQERHRRGSFAIKQRLRQRGINSSTLARIDMIEDPKDALELLLKKYSFWEDLD
                     YKEEIKRKNFLLGRGFSHETINKAMILAKNKKTTNKD"
     gene            9319..>9834
                     /locus_tag="VFD08_02610"
     CDS             9319..>9834
                     /locus_tag="VFD08_02610"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HZK09939.1"
                     /translation="MEIKEIKREKSEATVEYKVDFNEFKPYLDKVYKKERGRFMVPGF
                     RKGKAPRVLIERHYGEDIFYDEALNAMIPELYDKSIHQLDYEPVAQPQFSVEELDKER
                     GLVLKAVVTLLPEVKLGQYKNLEIEAVDTKVSDEEVMDKLKEEAEKNARMVPVDVAQE
                     EDIVTIDFDGSI"
BASE COUNT         3022 a         1841 c         2295 g         2676 t
ORIGIN      
        1 gataaagagg agtcccctta ctagggtggg atttactaag gtaaatggga ccctaaaaaa
       61 gcttagatta tggacttttt ggccatagtt ttgcttactt taatttattc ctaaagtaag
      121 ggggctttgt tttttaagct aagtgggaca cttgaaaaag ttggtgttgc gtttactttg
      181 gaaagttagg ccgaaaataa agcttgtaat attacgaaaa aaggccttgt agcttgctag
      241 tttgcatgtt acaataactt ttgcagcagc tctctgccct ttttataaga aagagggcca
      301 gaagaccaaa gggaggttaa aaatgaataa atacgaaagc attgttgtat tccgtcctga
      361 tcttgaagaa gacgcacgtg tgaagcttct tgaacgcttc aaagagatta tcgggcgcta
      421 tggcaaggta gaatcggttg atgactgggg tatgaaaaga cttgcttatg agatagaaaa
      481 gctgacagat ggctactact atctaattaa ctttgaagca aacagtgatt tacctgagga
      541 acttgagcga aactacaaga ttcaagacca agtaatccga tacaatatta tccgtaaaga
      601 cgcataagga ggaactatga accaagtcat attaattgga agactcacag cagaccctga
      661 attacgtttt gctgctagca caggaagagc tgttaccaat tttcgcctag ctgttgatcg
      721 ccctttttct agagaaaaaa agaccgactt ctttcgagtc gtagtctggg gtaagcaagc
      781 agaggccgtg gctaagtact tgcgaaaagg ttctcagtgt gctgttaatg gatctatcca
      841 aattgatcaa tataccgatc gagaaggtaa cacacgttat tctacagata tcgttgctaa
      901 tcaggtagag tttttaggta gtcctggtgg agctcgtaag gaaagttcaa atgatagcga
      961 ttcctacgag agtcctaatg atgcggacac agactttccc ttagtggacg atgaagacat
     1021 accattttag gaggaatcat ggcttttaga ccaagaagaa aaaaggtatg ctacttctgc
     1081 acaaataaaa tcgatgatat cgattataaa gatgttagaa cactaaagcg ttacgtttcc
     1141 gaccatggga aaatacttcc ccgtcgtgta acgggtgctt gtgccaaaca tcagcgcaaa
     1201 gtggctttac aaataaaacg tgcacgtgca gttgcacttt tgccttatgt agattaaacc
     1261 tcggaaattt ccgaggtttt tttctactgc acttgtgtct tttattaatt agggtagatg
     1321 gcgggaaaaa aagttatacc aattatcaaa ggcaattttt tgggttattt ccgggccgaa
     1381 tttatcttcc agggctccta ggagctgctg ggcctgtcca tggtgggcaa tcacctcagg
     1441 cttgataatt tcatctccct taaaaacgct aaggcaagaa cataaatcaa gaccaagtcc
     1501 gaccttgtcc tggcccagct tttttattaa atagtccata tgggcaataa agtcttctat
     1561 agaagaagtt ttttcgtccc ggctaataat aaaatgggct ccatttactc ccaggccaaa
     1621 atcctttttc cttaagatat caaggtactt gtccttgagg cttctaggag taggagtaat
     1681 gctatttgca ttgctgtggg aggcaataag ggggatatcc agccttatga tatcttctag
     1741 accctcatcg cttaaatggg acacatcagg gatgataccc agcttgtggg ccagttccat
     1801 cagctcatgg cctgctggac taaggccccc ttttttacta gggccctgga caaagtcaca
     1861 tccatcggca tagatatttc gccggctcca gctaaggcca agcaggcgga ctcccagctc
     1921 ataaaagaca tagaggtctc tgggctttaa gaggggttct gctccttcaa aggaaagaag
     1981 caggccaatt ttatccgagg cctggcagag ggcaaggtcc tcttttgtgc gaataagcat
     2041 aagtagttct gggctttctc ttagctcctc ataaaaaaag cccagctgct ccatggcata
     2101 gtaaaagggc tgatttagtt catggccctg tagataaata gaagcaataa caattttaac
     2161 tcctcccttt ttaaagcgag gataaaagtc gtctcgaaaa acatggctct tgccctgctt
     2221 tcttttaaag gaaatttcac ttaagagatc aaaatgggca tcaaataatg ggatcatagc
     2281 cacctccaaa aagagcatag caaataaaag cagctttttc aaatccttta taaaagaaca
     2341 aataaggact aactttttag aaaaaaaggc cagtatttct ttaaataaag agaaagaagt
     2401 cgcttttggc caaggaagtg gcacttatag gttagaagaa ggctttgtgt ggtataatta
     2461 aacgatgaac aaaaaagaaa gaaaagggtt tataatgaga aaaaaagaca attatagcat
     2521 gcttatggat ctgtatgaga tgaccatgag catgggttat tttgagagtg gctttaaaga
     2581 taccattgtt tattttgata tgtttttccg cagaactccc gataaggggg gctttgccat
     2641 tgccgctggc ctggcccagc tgattgagtt tctaaaaaat cttcatttta ccgaagagga
     2701 tattagctat ctgcgaggcc tcggaattga taatgaagac tttttagact atgtgcgtaa
     2761 gtttaaattt actggcgata tttgggcagt agaagaaggt actcctgttt ttcccggaga
     2821 acctctagtt acagttaggg ctcctgtgat tgaagcccag ctgatagaaa cagccctgct
     2881 tgtgattatc aaccatcaaa ccctgattgc tacgaaggcc aaccgcattg taagggcggc
     2941 ccagggcaag tccattattg aatttggagc ccggcgggcc cagggcagag atggcgccta
     3001 tatgggagca agagccgcct acataggagg ttgcattgga acgagcaacc taatggcagg
     3061 aagagacttt gacatacctg tttatggaac catggcccat agctggattc aactttttga
     3121 tgatgaagaa gaggcctttc gtgcctatgc taagacctac ccccaggcct ctagttttct
     3181 agtagatacc tacaatacct taaaaacagg agtccccaat gccatcaagg tctttgatga
     3241 ggtggtggtt cctagtggcc ataggccctc ttcgatccga cttgatagcg gggacctagc
     3301 ctacctctca aaagaggcaa gaaaaatgct ggaccaggca ggctatcccg atgtaaagat
     3361 tatggccagc aactctattg gggagcatac cctgcaagac cttcgtgacc aggatgcaga
     3421 gattgacctc tatggggtgg gagaaagact gattaccgct tcaagcgatg ctgtgtttgg
     3481 tggagtatat aagctggtgg cccttgaaga caagggagta attgagccta agataaagat
     3541 atctgaaaat gtagaaaaaa ttaccacccc tggttttaaa gagctttata ggatctatga
     3601 tggggttagt gaaaaggccc tagcagatct gattactctt aggggagaag aaattgatcc
     3661 aagccagccc tatacactct ttgacccagt ccatacctgg aaaaagacca gggtggaaaa
     3721 ctttatattg aaaaaattac ttgtcccggt atttaagggg ggcaaatgtg tttatccaga
     3781 gaagagcata caggaaattc gcgcttaccg agaaaaagaa gaagacaaac tatggaatgg
     3841 cgtaaagaga ataaaaaatc ctcataagta ctttgtggat ctttctcaag aactctggga
     3901 taccaagcag gatcttttgg taaggtatgc taagagggga cgttgatgaa aaaaagtaaa
     3961 agtaatctac tattaggact tcttggacta tctgttatag tgggaatctt tagtggattt
     4021 ataccgacaa tttttgttta tagtcttagt ttgctttttg cctatgttag cacgcgctat
     4081 ggggatgttg gttatgtttt gccaggactt gctatgatgc ttttgggagg tctgttttta
     4141 tatggaccct ccataagcct ggtggaaatt ggtcttttga tgctttatgg agtctttatg
     4201 gggatgagtt tctctaaaag gctccgtcca gaaatgatct actttcccac cctggccatt
     4261 ctttttgcca ttatgctttt aacttttttt cttcaaaaaa gcttactggg agtggatggt
     4321 cttgaagccg ttattgacac ctatatgaat caggttcaag ccagtgtaaa tgaccctcag
     4381 ctgctgataa atttaagggc taccataaag gaatttagtc tttctattgt ttttcttgtg
     4441 gctgttatgc ttaatctatt tctcttatgg atctttagca agatcttgga tataagaaaa
     4501 cttaaaaata cgggtcgctt tcattttgag ttttttcgcc taagagaact aaagtttttt
     4561 cagattatac aacttattgg acttgttatt ttaggaagcc tacttactgg gatgcctacg
     4621 caaacagcct tgggcaatct ggtttttatc ttaattgcta tattttatct gcagggccta
     4681 agcctatcga tctttttact taaaagccga aaaaggggca gaatccttac agcccttgtg
     4741 gtcctcttat cactaacggt tccctttata cagcttttct tgcttatact gggtctaata
     4801 gaacaaagta aagattttag aaagttggag aaagttctat gaaagataaa aagaaaatca
     4861 cagaattata ccgcagaaat acatggatct atcttttttt cctagccctt ttaattgcat
     4921 ttatttttat tggcaatagg gatctaggtt atgtagctct gggactttat ctgattattt
     4981 ttagctatac tctttatttt cagctgcgaa gccgaaatca atgggagacc atgctagaag
     5041 acttgcgcct gcgggtggaa gagggtaata tttcctccta cctaaatatg cccctgccca
     5101 ttcttcttgt tgatgaaact ggaatgatta tttggcataa tagtaaattt aaaaatattt
     5161 ttgatagtga aacaggtttt attggacaaa agctggaaac aatttttcct cttttaaact
     5221 ggaaccaggt tatggaaggc ccctactttt tcaatcaagc cttgggggac aagcagttta
     5281 gggtctttcg ctcctcaacc aggaataaag gggtaaatat ctataccctc tactggtatg
     5341 atgagacaga ttttgctaaa ctcaagcagc gctataagga cgaaagaccc ctggccgttt
     5401 atatccagct ggataatttt gatgaggtag taaaagaaac cgacgagagt cgtcttccct
     5461 ttttgctctc aggaataaat agtgttattc gaaaatgggc cctagatcat cagggaatta
     5521 ttcataaaat taatgaggat gagtaccttg tcctggtaga aaatagtttt ctggcctcta
     5581 tggaatcaag aaagtttacc attattgatg atgtgcgaaa aatagaggaa ggaaaccgct
     5641 atcctatgac cctttctgtg ggagtgagtg ccgatggagc aagtttttcc gagcgagatg
     5701 aaggaagcca gcaggcacta gaactagccc tgggtagagg gggcgaccag gtcgtggtaa
     5761 gaaaagacga caactacgaa ttctttggag gacgtagtaa aaatgtagaa agaaaaagcc
     5821 gggtaaaaag ccgggttgta gcccaggggc tggctggcct aattaaggag agcgagcggg
     5881 tttatattat gggacacaat tatggggata tggactcctt tgcttcgacc ataggtatgt
     5941 accgggcctg tcttaattta gatgtaccgg cctatattgt tcttaataaa tctaccgatg
     6001 ccattgaaga actctatgct ctttttagag ataataagct ctatcgcttt attagcgggg
     6061 cccaggctgc aggggctcgt aaccatgata aggacctcct tattgtggtg gatacccata
     6121 ggccaaattt cactgaagca ccagaactac tgcctctttt tgagaggatt gtagtgattg
     6181 atcatcaccg caggggggca gagatgatag agaatacttc tttactctac caggaaccct
     6241 atgccagtag tgcagcggag atgattagtg agatcctcca gtatattaca agcgatgtgg
     6301 ttcttgagcc cctagaggcc agtgccctgc ttagtggaat tgttcttgat acaaagaact
     6361 ttgtctttaa tacgggagtt agaacctttg atgccgcctc ttttttacga agacatgggg
     6421 cagataccag gctggttaga ggactctttg aagaagacct tcttgaatca gtcattaaaa
     6481 gcaatattgt aagttcagcc cttttaatag aggagggcat cgccctttct cagagcagtg
     6541 agaactctgc taatattaaa accattatca gccagggagc ggatgaactt atcgatataa
     6601 agggaattca cacttctttt gtactgggtt gtgatttaga aggaaaggtt tatatttcag
     6661 ccagaagcct tggagatacc aatgttcagg taatactgga aaaactaggc ggaggcggcc
     6721 atctagaaac cgcaggagcc cagctagaag acacaacaat agaagaagcc aagactcttc
     6781 tgcttgaaac aataaaggaa agtttggagg aataaatgaa aattatttta ttagaagatg
     6841 taaaggatat gggaaaaaaa ggcgatatgg ttgaggccaa ggcgggatat gcaagaaact
     6901 atcttatccc tagaggacta gcagccgaag caacccaatc aaatgtcaca gaactcaaac
     6961 accaaagaca tgcaaaaagt aaaaaggcag ccaaggccct agctagggcc caggaactaa
     7021 agggcaagat ggaagaagaa gttctaaaga tgaaggtaaa agcgggcgaa gggggcagga
     7081 tcttcggttc tgtaaccacc atggacattt ctgcgggcct aaaagaacta ggctatacag
     7141 tagataaaaa gcgagttgtc ctagaccagc cccttaagac cctaggagag cataaggtaa
     7201 aactaaaact ccatcatgag gtggaggcag aaattcgact tcttctagtt ggagaagaat
     7261 gatgaatttg ccccatagca tagatgtcga aaaagccctt cttggcagta tgctacaaaa
     7321 taaaaaggcc atgagtattg gcattgagca gctggaggcc tatgattttt atgatgaggc
     7381 caatggccta atcttttctg ccattgtcag cctggccgat aaaagcgata gcctagatat
     7441 taccatgcta agggattatc tagaaaataa aggaaccctg cttcgggtag ggggggtaga
     7501 gtatcttatc cacctaaccg aggcaggcct gcgcccctat aatgccctta gctatatcca
     7561 gtcaatcaaa gaaaaaagtg ttcgccgaaa gcttattgag gccgcctcgg aaataacaaa
     7621 gatttctaag gatacaacac aagatctgga cgttctaggt gaggccgagc ggcgcatctt
     7681 taacctggcc gatgaatcca cccagcaggg gctggttcat gtggcaagtg aactaaccga
     7741 tatgatggac catctagaag aggcaagtgc caatcccgaa ggcatgcggg ggctttccac
     7801 gggctttatt gaactggatc taaagaccaa tggccttcat aagggagacc ttgttctcat
     7861 tgcagccagg ccctctatgg gaaaatctgc tttagctatg aatatagccc agcatgctgc
     7921 cctgcatgaa aataaaaacg tttgtgtttt tagccttgaa atgggaaaat cttccctcct
     7981 ataccgggtc ttagcatcgg aatatttgat ttcactgaca aatatcctaa cagccaaact
     8041 atcaggggag gattggaaaa aactttctag aggggtggga gctctttcca atgcacctct
     8101 atatatagac gacacttcgg gtattagtgt tatgcagatc cgaagtaagc ttcgtcgcca
     8161 ccgcctagaa catggagaaa ttgaccttgt tgtagttgac tacctgcagc taatggatgg
     8221 cgggggagga gaaaataggc aggtagaaat agctggcata tctagaggcc taaaggccct
     8281 agccaaggaa tttaattgtc ctgtcctggc cctctcacag ctgtcgcggg gaccagaaca
     8341 aagaagcgat aaaaggcccc ttctttctga tctaagagag tcgggcgcca ttgagcagga
     8401 tgcagatatt gttatgttta tctaccggga agattattat aataaggata cagagcttca
     8461 aaatataagt gaaataatta tttccaagca gcgcaatgga gagaccggaa cggtccagtt
     8521 agcctggctt ggtcagcaca caaaatttgc cgatcttcat cggggatagg gagtaaggat
     8581 gaataataaa agtaaaaaac taattgctaa aattcttgta ggtcttcttg tttttgccct
     8641 agtggcgccc tttttggcag gacttttcta gtgattgaaa aagcagactg gtcaaataaa
     8701 ggcgtgaaaa tcactttgga tggcgaagag tatctaattc acaccaaggc ctatatggaa
     8761 gcaaatcttt ctgagggcca ggaagtggat cttgatgctc ttcttctgcg ttctgcttat
     8821 ttctatggac ttgaaaaagc cgagagctat ttgctccggg gcctgcgcac gcgaaaacag
     8881 ctaagggact atcttatagc aagagaattt attgacccag tggaaaacat tctaaacttt
     8941 ttagaggaat acggccttat aaatgattat gactatgcca agacctatta tgaacaagag
     9001 cgccaccgca ggggaagttt tgccattaaa cagaggctta ggcaaagggg aataaattct
     9061 tctaccctag caaggattga tatgatagaa gaccctaagg acgccctgga gctactcctg
     9121 aaaaaatata gtttttggga agatttggat tataaagaag aaataaaacg caaaaacttc
     9181 ctcctaggga ggggttttag ccacgaaacg ataaataaag ccatgatact tgcaaaaaat
     9241 aagaaaacaa caaataaaga ttgattaatg gggtagttta taatagaatt atccttggaa
     9301 aaaaatcagg aggaaaaaat ggaaattaaa gagattaaaa gagaaaaaag cgaagctacg
     9361 gttgaataca aggtagattt taatgaattc aagccatatt tagacaaggt ctataaaaaa
     9421 gaacgaggca gatttatggt tcctggattt agaaagggaa aggcccctcg tgtcttgata
     9481 gagcgccact atggcgaaga tattttttat gatgaggctc ttaatgccat gatccccgaa
     9541 ttatatgata agagcattca ccagcttgat tatgaaccag ttgcccagcc ccagttttct
     9601 gtagaagaac ttgataagga aagaggactt gtgcttaagg cagtggtaac tcttctgcca
     9661 gaggtcaagt taggccagta taaaaaccta gagatagaag cagtagatac aaaggtaagt
     9721 gatgaagaag taatggacaa actaaaagaa gaagcagaaa aaaatgcccg tatggtgcca
     9781 gttgatgtgg cccaagaaga agatatagtg actatagact ttgatggtag catt
//
LOCUS       DASRNA010000096         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_624922,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000096 DASRNA010000000
VERSION     DASRNA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624922"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..914)
                     /locus_tag="VFD08_02615"
     CDS             complement(99..914)
                     /locus_tag="VFD08_02615"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZK09940.1"
                     /translation="MLNLIKAIIFGIVEGITEWLPISSTGHMILLDEIIKLQVSEAFM
                     EMYLVVIQLGAIMAVVVLYWQDIFPFKFKPFSVERQTMIMWFKILFASIPAGVIGILF
                     KSTFYKYFYNSVSVALALIIVGVLFIYLENKNKDREAKIVSIRKITYKDAFIVGIFQL
                     FAAIFPGTSRSGATILGGIYLGFSRQIAAEFTFFMAIPAMFGASVLELRNFGMFSFFE
                     FFILIVSMVVSYLTSVYVIRFLMNYIKKHDFKAFGWYRIGLGLLVLLVSFITK"
     gene            complement(908..1264)
                     /locus_tag="VFD08_02620"
     CDS             complement(908..1264)
                     /locus_tag="VFD08_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK09941.1"
                     /translation="MIKRKEEMKTREVDCLMDGQGRVSFLDIFDKEEMLGLARLFAIV
                     TLEQGCSIGSHIHKGEAEIFLLLEGAAQTLDNGEWVELRKGDAAFTREGYEHSLRNPY
                     KETVKLLAVIIEGDSC"
     gene            1334..1831
                     /locus_tag="VFD08_02625"
     CDS             1334..1831
                     /locus_tag="VFD08_02625"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HZK09942.1"
                     /translation="MKVGLRVIKTVLAVALCFLIDAFRGGMPFFAVISAILCLQKGKV
                     ETYSKAITRVLGTLIGGGYGLFSLLLFQYTGVAYKSPVYVLVISLMAILIINTNLWIK
                     NPISVGFSCVVYYSVVIGHFQDLDPFLFVRNRMLDTFIGIGVALFLNYIFPFKGETKD
                     PMLSQ"
     gene            complement(1794..2405)
                     /locus_tag="VFD08_02630"
     CDS             complement(1794..2405)
                     /locus_tag="VFD08_02630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZK09943.1"
                     /translation="MIKTVIFDLDGTLLDTLEDLYQAVSHAFTLYGYKSPNKEEVKKA
                     LGKGPLVLMQRLGAKDPELFLEAFALYYHEHLDVYTRPYPGIMELLDELDKKEIEMAV
                     LSNKPHEHLVRICDIYFKDRFFAVMGSGAGFPRKPDPTSLLHLMNKIGRQKNELLYVG
                     DSEVDILTAKKAKCRGLQVSWGFGSLVKDADFIEKAWDLLSHL"
     gene            complement(2402..>3477)
                     /locus_tag="VFD08_02635"
     CDS             complement(2402..>3477)
                     /locus_tag="VFD08_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09944.1"
                     /translation="PNLGTFHVEVTINSWEIPGRKILLNSQCFITEFLVDDKENFLLP
                     KKEASMDSYHLPTGHRYKISYQLYVNPRRPWLFIRDDGTYLPLFGEDAPPLRHTEIIL
                     PEDLVVLSSHELEKVQRRQGFLRFLFKGEDGPTFSIAPYHADRIFSGEIYLLDDKPDV
                     ELLSLVLRRAGEFMRKHCGESSFTGDLRYVSLPEGEKPFQLKGSFFFPWPKKTSLRGF
                     EEVLKAYLKLGWTVPAYEAEEFFGDGFVLFYTSKIIKNIYSPQEIESFNEDHKEGRNR
                     VKTKSLNQLKGRDLSLGGWMLLEDLEAFLGPDIFYPVIKVFLEKHQQKPADLEYLLEC
                     FTSLSWKEKTKEFLEPRLFGGGI"
BASE COUNT         1052 a          770 c          588 g         1067 t
ORIGIN      
        1 catatatccc ctatcttatt atactctatt attctaaaaa ctacagacaa aaatcaaata
       61 cttggcatcc tcgccctaag agccttctct tactcaactt attttgtaat aaatgaaacc
      121 agtagaacaa gaagcccaag accaatccta taccagccaa aagctttaaa gtcatgtttt
      181 ttgatgtaat tcataagaaa acgaattaca tagacgctgg ttaaataact gaccaccata
      241 cttacgatca aaatgaaaaa ctcaaaaaaa ctgaacattc caaaatttct aagttccaga
      301 acagatgcac caaacatagc tggtatggcc ataaaaaaag taaattctgc cgcaatttgt
      361 ctactaaatc ctaagtaaat ccccccaaga atcgttgccc cacttctaga ggttccaggg
      421 aagatggcag caaaaagttg aaaaatcccc acgataaagg cgtctttata ggtgattttt
      481 cttatactaa caatcttagc ctccctgtct ttgtttttgt tttccaagta aataaaaaga
      541 acacctacaa tgatcagggc tagggccaca gagaccgaat tataaaagta cttataaaaa
      601 gttgacttaa aaaggatccc aattactcca gcaggaatcg aggcaaaaag gatcttaaac
      661 cacataatca tggtttgtct ttccacacta aaaggtttaa acttaaaggg aaagatatcc
      721 tgccagtaaa gaaccacaac agccataatt gctcctagct gtattaccac cagatacatc
      781 tccataaaag cttcgcttac ctgtagctta ataatctcat caagaagtat catatgccca
      841 gtactgctaa tagggagcca ttctgtgatg ccctcaacaa taccaaatat aatggcttta
      901 atgagattta gcatgaatct ccctcaataa ttacagcaag aagctttact gtttctttat
      961 aaggatttct aagcgagtgt tcgtatcctt ctctagtaaa agctgcatcg ccttttctta
     1021 actcaaccca ctcaccatta tctagtgttt gagcagctcc ttctagaagt agaaagatct
     1081 cagcttctcc cttatggata tgggagccaa tagagcagcc ttgctccaag gttacaatag
     1141 caaaaagcct ggctaagcca agcatctcct ctttgtcaaa aatgtctaga aaagaaaccc
     1201 tgccctggcc atccataaga cagtctactt ctcttgtttt catttcttct ttgcgtttta
     1261 tcattgattc ctcctggttt agaattttat tttttctatt atttatgtta agctaaggca
     1321 attgaggtgt aatatgaaag tcggacttcg cgtaataaaa accgtcctcg ccgttgcttt
     1381 gtgttttctt attgatgcct ttcgaggggg tatgcctttt tttgctgtaa tatcggctat
     1441 tttatgtttg caaaaaggca aggtagaaac ctattccaag gcaataacta gagtccttgg
     1501 tactcttatt gggggtggct atgggctatt tagcctccta ctttttcaat atactggtgt
     1561 tgcctataaa agtccagtat atgtccttgt tatttctttg atggccattt tgattatcaa
     1621 taccaacctt tggattaaga accccatttc cgttggcttt tcttgtgttg tttactactc
     1681 tgtggttatc ggacattttc aggatttaga tccctttctt tttgttcgta accgcatgct
     1741 ggacaccttt attggcattg gagtggctct ttttcttaat tacatctttc cctttaaagg
     1801 tgagacaaaa gatcccatgc tttctcaata aagtcagcat ccttaacaag acttccaaaa
     1861 ccccagctta cttgcaggcc tctacatttg gccttttttg ctgttaagat atccacttca
     1921 ctatccccca cataaagaag ctcgtttttt tgcctaccaa tcttattcat taaatgcaaa
     1981 agagatgtgg gatcaggttt tcttggaaaa ccagcaccac ttcccattac cgcaaaaaaa
     2041 cgatctttaa agtaaatatc acatatcctt acaaggtgtt catggggctt attgctaagc
     2101 acagccattt ctatctcttt tttatcaagt tcatcaagaa gttccataat acctggatag
     2161 ggccttgtat agacatccag atgttcatgg tagtaaagag caaaggcctc taaaaagagt
     2221 tccgggtcct ttgccccaag tctttgcatt aaaaccaagg ggccttttcc caaggccttt
     2281 ttaacctctt ctttattagg acttttatag ccatataagg taaaagcatg gcttaccgct
     2341 tgataaaggt cctctagtgt atctaaaagt gttccatcta aatcaaaaat tactgttttt
     2401 atcaaatccc tcctccaaat agtcgaggct ctagaaactc ttttgttttt tccttccaag
     2461 aaagactagt aaaacattcc aacaagtatt ctagatccgc aggtttttgc tggtgttttt
     2521 ctaaaaaaac tttgatcaca ggataaaaga tatctggacc aagaaaagct tccagatctt
     2581 ccaggagcat ccagcctcct aggctaagat ctcttccctt aagttgatta agactcttgg
     2641 tctttacccg gtttcttcct tctttatggt cttcattaaa actctcaatc tcctgaggac
     2701 tatagatatt tttaattatt tttgatgtat aaaaaagaac aaagccatcg ccaaaaaact
     2761 cctccgcctc ataggctggg actgtccagc ccagttttaa gtaggccttt aacacctctt
     2821 caaagccacg aaggctagtc ttttttggcc agggaaaaaa gaaacttccc ttgagctgaa
     2881 agggcttttc tccctcaggc aggctcacat agcgaaggtc tcctgtaaag gaactttctc
     2941 cacagtgttt tctcataaac tctcctgctc ggcgtaaaac taggcttaat agctccacat
     3001 caggtttgtc atcaagaaga tatatttctc ccgaaaaaat ccgatcagca tgatagggag
     3061 ctatactaaa ggtcggacca tcctctccct taaaaagaaa gcgaagaaaa ccttgtcttc
     3121 gttgaacttt ttctagctca tgactactaa gcactactag gtcctcgggg aggataatct
     3181 ccgtatggcg aaggggaggg gcatcttccc caaaaagcgg aagataggtc ccatcatctc
     3241 ggataaataa ccaaggccgt cttggattaa catataattg ataagaaatc ttatagcgat
     3301 gacctgtagg aagatggtag ctatccatag aagcttcttt tttaggcaaa agaaagtttt
     3361 ccttatcatc gacaagaaac tctgtaataa aacactggga gttgagcaaa attttgcgcc
     3421 ccggaatctc ccaactattt atcgtcacct ctacatggaa agtgcccaaa ttagggc
//
LOCUS       DASRNA010000097         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_625465,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000097 DASRNA010000000
VERSION     DASRNA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625465"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFD08_02640"
     CDS             <1..385
                     /locus_tag="VFD08_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZK09945.1"
                     /translation="KKRIQRIEDADLFTGLDLILDGLDSISSRYLLMEIAIKKGLPLI
                     HGAIGAFEAQLAFLEPDINRMKVIYPWEEEVSKANLSFVPSFLASLQVKMLLEYFLSR
                     PELEKNTLIRIDLRDLQIYKIPLDT"
     gene            complement(382..603)
                     /locus_tag="VFD08_02645"
     CDS             complement(382..603)
                     /locus_tag="VFD08_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZK09946.1"
                     /translation="MIEIRLFAHFRNGREKISFQPVEDFPTPAHILKHLEIEQKKVAI
                     LLVNGFHSKFDHPLKEGDIVAIFPPVAGG"
     gene            complement(603..2408)
                     /locus_tag="VFD08_02650"
     CDS             complement(603..2408)
                     /locus_tag="VFD08_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZK09947.1"
                     /translation="MMKTTYNLKLLRIDLSKGTIKEETIDKDQAHKFIGGRGLATKWL
                     YDDGIQNVDALSEDNKLIFITGPLTGSNAPTAGRYMVVTKAPLNGLIGSANSGGVWGA
                     KLKYAGYDALIVEGKSEKPVYINIDDENFEILDASEIWGKTSIDVDAYLKQKHGNKTS
                     ALYIGPAGENQSLLSCIINDKDRAAGRGGMGAVMGSKNLKAISVKASRNIMEAYDVEA
                     MKEAFKLSLDKIRENPVTSGGLPTYGTAVLVNIINNIGAFPTKNWQLSYFEDADKTSG
                     EAMVEENVLVKNSFCHRCPIGCGRVVRMEGQEIGGPEYEPLWAYGGDCFVSDLEAISE
                     ANHLCNEYGLDAIGAPCTIAAAMELYQRGYIKEEELGGVSLDWGEGNSIIEWTRKMGK
                     GDGALAKLMADGSFKLCEHYGAPEFSMSSKKQELPAYDARAIQGIGLNYATSNRGGCH
                     VRGYTISPEILGLPVAADRTIVEGKAGLVKVFQDLTAVIDALGLCLFTSFALGAPEYT
                     ALYNAATGSSLSVDEVMEAGERIYNIERQFNLEAGMKAEEDTLPNRLLKEPIANGPSK
                     GMVSRLDEMLPEYYELRGWVDAFPTEETLKRLGLK"
     regulatory      complement(2466..2577)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
BASE COUNT          758 a          601 c          544 g          801 t
ORIGIN      
        1 taaaaaaaga atccaaagga tcgaggatgc agatcttttt actggcctag atctaatcct
       61 ggatggcttg gattctattt cttccagata tttattgatg gagattgcca taaaaaaggg
      121 tcttcccctt attcatggtg ctatcggtgc ctttgaagcc cagttagcgt ttttagagcc
      181 tgatatcaat cgtatgaaag ttatttatcc ctgggaagaa gaggtctcaa aggccaacct
      241 ctcctttgtc ccaagttttc ttgcttcctt gcaagtgaaa atgttacttg aatattttct
      301 ttcaaggcca gagctagaga aaaacacctt gatacgaatt gatcttagag atttacaaat
      361 ctacaagatt cctctagata cttagccacc agcaacaggc gggaaaatag cgacgatatc
      421 cccctctttt aaggggtgat cgaattttga gtgaaagcca ttcacaagta gaatggctac
      481 ttttttttgc tcaatttcca gatgcttgag gatatgtgct ggtgtaggaa aatcttccac
      541 cggttgaaag cttatctttt ctcgtccatt acgaaaatga gcaaatagac ggatctcaat
      601 cattatttaa gtcctaagcg cttaagggtt tcttctgttg ggaaggcatc aacccatccg
      661 cgcagctcat aatactctgg gagcatttca tctagtcttg aaaccattcc tttagaagga
      721 ccatttgcaa tgggttcttt taaaaggcga ttaggcagtg tatcttcttc tgctttcatg
      781 cctgcttcta aattaaactg gcgctcaata ttgtagatgc gctctcctgc ctccatgact
      841 tcatccacac ttagagaaga acctgtggcg gcattgtaaa gggcagtata ttctggtgca
      901 cctagggcaa aggaagtaaa taagcaaaga cctagggcat caattactgc agtaagatct
      961 tggaaaacct taaccagtcc agccttacct tctacgatgg tgcggtcagc agctacgggt
     1021 aaacctagga tttctggaga aatcgtatat ccacgaacat gacagccccc tcgatttgag
     1081 gtggcatagt ttagaccaat tccctgaata gcgcgagcat cataggcggg caattcttgt
     1141 tttttagagc tcatggaaaa ttctggagcc ccataatgtt cacagagctt aaaggagcca
     1201 tcagccatta atttggccag ggcaccatcg cctttaccca ttttcctcgt ccattcaata
     1261 atggaatttc cttctcccca atcaagactt actccaccta gttcttcctc ttttatataa
     1321 cctctttggt aaagttccat ggctgctgca atggtgcaag gagccccaat agcatcaagg
     1381 ccgtattcat tacaaagatg gttggcttca ctaatggctt caaggtcgct aacaaaacag
     1441 tcgccaccat aggcccataa aggctcatat tcaggtccac cgatttcttg gccctccata
     1501 cgaacgactc gtccacaacc aatagggcag cggtggcaga aagagttttt aacaagaaca
     1561 ttttcctcta ccatggcttc gccactggtc ttatcggcat cttcaaagta ggatagttgc
     1621 cagttttttg taggaaaagc accaatgtta ttgataatgt tcacaagaac cgctgttccg
     1681 taagtaggca ggccgccgct agtaacaggg ttttctctaa ttttatctag ggacagctta
     1741 aaagcttctt tcatagcttc tacatcatag gcttccatga tatttcgtga agctttaaca
     1801 gaaatcgctt ttaggttttt acttcccata acagctccca tgcctccgcg accggcagcc
     1861 ctatctttat cattaatgat gcaggaaagt agagattgat tttctcctgc tggcccaata
     1921 taaagagcag aagtcttatt tccgtgcttt tgttttaagt aagcatccac atcgatagag
     1981 gtttttcccc aaatttctga ggcgtccaga atctcaaaat tctcatcgtc aatattgata
     2041 tagacaggtt tttctgattt gccttcaaca ataagggcgt cataaccagc atacttgagt
     2101 tttgcccccc aaacaccgcc ggagttggct gaaccgataa ggccatttag aggtgccttg
     2161 gtaacaacca tatagcgacc agctgttgga gcgttggaac ctgtaagggg accggtgata
     2221 aagattagct tattgtcttc ggaaagggcg tcaacatttt gaatgccgtc atcatagagc
     2281 cattttgtag ctagccccct accgcctata aacttatgtg cttggtcttt atcgatggtt
     2341 tcttccttaa tcgttccctt gcttagatca atgcgtagaa gttttaaatt ataggttgtt
     2401 ttcatcatta ttacctccaa taaaaaatca gggtatgcga ccctgataat aaaataaggg
     2461 tcgcactaaa aaacagtgag atccctttcc aagcatggga ggccttgctg tttccaacaa
     2521 aacccatcgg cgcgctacca tttcactagg tagagacgct cggaatcatt tattacttac
     2581 attatagcaa aaaaacacct atatgtaaat aaaaacaaac aatccttttc aaaaaaaacg
     2641 agttgtattt tatttatagg ttttgcgcct tagctaaggc aggaaaatgg tttgagatta
     2701 gggg
//
LOCUS       DASRNA010000098        11983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1073928,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000098 DASRNA010000000
VERSION     DASRNA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073928"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..1178)
                     /locus_tag="VFD08_02655"
     CDS             complement(420..1178)
                     /locus_tag="VFD08_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HZK09948.1"
                     /translation="MSYKDVETKELFRLFKASPNNEIRDELYTRHIYIAEILTKKYVN
                     KGIDHEDLFQVASLGLLYAVQRFDVDKGFEFSSFATPTIVGELKKHFRDKGWTIRVPR
                     RIQELSKKITLARDQLYKSQGGPPTVNDIAEYLSVSDEAVIEAMEASKVYTPRSLDIE
                     LETGGEDRDVRLMDIVGEEDKNFEAINNEEFIHFIMDKLNPMERKIIEDRFFEEKTQI
                     VVAKELSVSQMTISRMEKKILAKFQEEYRKVIEI"
     gene            complement(1191..1583)
                     /locus_tag="VFD08_02660"
     CDS             complement(1191..1583)
                     /locus_tag="VFD08_02660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK09949.1"
                     /translation="MEIFKLNIPNKKEYVISARLAASSVAGIHGFDIEKVEDIRLAVG
                     EACNNVVLHGRGSKSIDIIIEIGKDFMKISVSDQGGGFVLEKINPPTPEDYMGSGLGL
                     YIIDSLMDEMIVESEEEKGTTIHMIKKR"
     gene            complement(1628..1948)
                     /locus_tag="VFD08_02665"
     CDS             complement(1628..1948)
                     /locus_tag="VFD08_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZK09950.1"
                     /translation="MAVKIRYLFSERGDCWEVKIMGEVDIHTSPEVKEKLEAILEEKE
                     KSMIINAEELDYIDSTGLGMLIGIVKRLKKNDLELVLAKPQSSVLKLFRITGLDKVIR
                     VREE"
     gene            complement(1932..4367)
                     /gene="gyrA"
                     /locus_tag="VFD08_02670"
     CDS             complement(1932..4367)
                     /gene="gyrA"
                     /locus_tag="VFD08_02670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZK09951.1"
                     /translation="MKEQIIERDISREMQKSYIDYSMSVIVSRALPDVRDGLKPVHRR
                     ILYSMYEQKYTHDKSFKKSARIVGDVIGKYHPHGDSAIYNAMVRLAQEFSTRYLLVEG
                     QGNFGSIDGDGAAAMRYTEARLSPIADELLRDLDKETVDFYPNYDESLMQPWVLPSRF
                     PNLLVNGSTGIAVGMTTSIPPHNLREIIDGTIYAIDYPECDTEDLMEIIKGPDFPTGA
                     TIMGKESIRQAYRTGRSRVRIRSKASVEEFGKGRSRIVITEIPYMVNKTKLLESIADL
                     VRTKRIDGITEMRDESSRKGIRIVLEVRRDANANVILNQLYKNSTLESVFSMIFIALQ
                     DKMPKVFTLKGLIEQYILHQKDVETRRIKYDLKKARDRAHILEGLLKALENIDEIIRI
                     IRSSYDDAREKLIESFDFTEIQANSILSMQLRRLQGLEYKKIEDELTGLQKDIAYYLS
                     ILGDESLLMDIIKESLEDIKERYGDERRTSIEHSYEELEDEDLIEERKMVITLTQRGY
                     VKRVAQDVYQSQNRGGRGIIGLTTRDEDVIYDIFTTSTHSDLLYFTNLGKLFRLKAYR
                     IPESSRQSRGTPIINLLQLDEGEKVTSIIPVNEMDDRRIVLATKEGVINKMLLENIES
                     NRQGGIKVIRLDEGDEVIHVRILDEDSDVFVATKLGKSIRFPSSEMRILNRNTRGVKS
                     IQLEKEDEVVSTEIVNEKEGDILTITSKGYGKRTELTNYRSQGRGGKGMINYKLSDKT
                     GHVVGVSMVTEEDELFLINSQGSMIRIRAMDVSKMGRNTSGVSLMRFEDEVEVVGFAK
                     ARETEEEEDGSED"
     gene            complement(4376..6268)
                     /gene="gyrB"
                     /locus_tag="VFD08_02675"
     CDS             complement(4376..6268)
                     /gene="gyrB"
                     /locus_tag="VFD08_02675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZK09952.1"
                     /translation="MSERYSADKIQVLEGLEPVRTRPGMYIGTTGQRGLHHLIYEIID
                     NSIDEAINGFADSISIVIHKDSSVEVKDNGRGMPTDLHPKLNISAVEVIHTVLHAGGK
                     FNNEEYKVSGGLHGVGAAVVNALSKNMIVEVYREGKIHRTEFARGIITRPLEIIGETS
                     EQGTRTIFWPDEEIFEEIEFDNKILENRFREMAFLNKGIKIDFIDHIHEKEVHFFYEG
                     GIAEFVKYINRNKTALHRKILYGEQVRDGVVVEIALQYSDTYTENIHTFANNINTQEG
                     GTHLSGLRAALTRVINEYGRKNGFIKENEDNLIGDDVREGLTAILSVKVSQPQFEGQT
                     KTKLGNSELRGITDSVCSEILSNFLMEEPQEAKFIMEKCIRSSKARIAARKVRDLTRR
                     KGLLDGFSLPGKLSDCQEKDPVLSEIYIVEGDSAGGTAKLGRERSYQAILPLRGKILN
                     VEKANFSRALNSREIRDMVTAFGVGIGDEFEIDKLRYHKIIIMTDADVDGAHIRTLLL
                     TFFYRYLTPLIENGHIYLAQPPLYSVKRGNKIEYAYTDNELQILIEKMGRNSHIQRYK
                     GLGEMNAEQLWTTTMDPENRILIRVNIDDAIEADQVFTTLMGDQVEPRRQFIQEHGVN
                     VTNLDV"
     gene            complement(6261..7328)
                     /gene="recF"
                     /locus_tag="VFD08_02680"
     CDS             complement(6261..7328)
                     /gene="recF"
                     /locus_tag="VFD08_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HZK09953.1"
                     /translation="MKINYARLINYRNFVDEEFYFSSDFEFILGDNAQGKTNLLEAIY
                     LACMGKVYRARSPREAFHERKDPFRVELNFSQGSEDFEFVYTHKDKKPVISVNNIEIE
                     RRSELFGRFPMVLFSPEHLKVLREGPSYRRKFIDREISLVSPAYFYSLIQYQRLLRSR
                     NILLKENFDPLQMDVYEEKLALYGKKIYKARGLFVEILNAQSKIVHEFISGGKEELCL
                     SYKSSFQGVEDPRKRYREDRKKDLSLGYTSFGPQSDDMYIEIDKRDARRFASQGQVRI
                     GSISIFFGLLPFITKQLEKKPLILLDDVFSELDRFRRHQLLELLQDYQVIISGTDLDG
                     LDLSKIKDFNKTTIVEGRKYE"
     gene            complement(7727..8782)
                     /locus_tag="VFD08_02685"
     CDS             complement(7727..8782)
                     /locus_tag="VFD08_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK09954.1"
                     /translation="MRINKIIRKKRLELGLTQKKVAEYLGVSTPAVNKWEKGVSYPDI
                     SLLPSLARLLKTDLNTLLSFKEELSEEEIGRFINELSSTIQKKGFEEGYSLAEEKIKE
                     YPTSPKLIYTIALTLEGSLLVFENKDSEKYGRQIESLYKRLAHSDDSKIRDQAISKMI
                     SKYIKRAEYEKAQKLIDSLPEQGIDKGQIQTSLYIKQKKNIEAMKLLEEGLLEKINEI
                     QGLLLKLAQVNQKEKKIEEADYIAKVFEKTARLYDLWDCSAYIANFELAVFTKDQEKS
                     LALLKNIFEAMDRKWDINHSPLYRNIKTKKEAGRMGEFFLLSLIKEIEKNKKYEFLWK
                     NKDYLDLIKRYSPRLSN"
     gene            complement(9000..9731)
                     /gene="nagB"
                     /locus_tag="VFD08_02690"
     CDS             complement(9000..9731)
                     /gene="nagB"
                     /locus_tag="VFD08_02690"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HZK09955.1"
                     /translation="MEIKIYKNYEEMSRAATRILAVQMLKKPDSVLGLATGSTPKLTY
                     KRLIRLYDEGLISFKKIKTFNLDEYIKIDPKNPGSYRAFMENKLFNKVDIDEKHWFIP
                     DGNAEDIEKECLLYDKKIKDMGGLDIQLLGIGTNGHIGFNEPGTSFSTRTHVVELKEN
                     TIKDNSRFFNKIEEVPSQAITLGIRSIMEAKKILLIANGANKARAIKGTLEGPITTDL
                     PASVLQLHPNIVVLLDEDAARELGR"
     gene            complement(9742..9975)
                     /locus_tag="VFD08_02695"
     CDS             complement(9742..9975)
                     /locus_tag="VFD08_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZK09956.1"
                     /translation="MIVKIKTDHIRLDQLLKFAAIVDSGGLAKHLIRDGFVYLNGTTC
                     TQRGKKIFSGDLVEVIIAKEGIHERVEVVLEEK"
     gene            complement(9972..11069)
                     /gene="dnaN"
                     /locus_tag="VFD08_02700"
     CDS             complement(9972..11069)
                     /gene="dnaN"
                     /locus_tag="VFD08_02700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZK09957.1"
                     /translation="MKFTLNQRELVSALSIVSKATNLRTTMEILRGIHIEAKDNMIIL
                     KSHDLSLSIIMQVPAFVEKEGSVLIDSSLFSDIIRKLPGEPITMEIKDESLFLVCQSS
                     RYRLSMMDQENYPQIPQINEGSSIKIEQGAFSSLVKMTKFAVGQDETRPILTGSLVEV
                     KDGKMKMVSVDGFTIAIKTVPILEGNPCQVVIPGRNLSEIEKIIGTGEEEMEILVSSN
                     HVVFNFDGITIISKVLEGNYINYERVIDTGFDTKVEIPIDEFYRALDRVALLANRSKS
                     YIVNFDFSDRQVDISSSSELGLAKESISTVNHGEPLVINFNPNYLLRALRVMEEDSMV
                     FAMGGSHKPCKITQKDKDDYEYYVVPIRTPQ"
     gene            complement(11265..>11983)
                     /locus_tag="VFD08_02705"
     CDS             complement(11265..>11983)
                     /locus_tag="VFD08_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZK09958.1"
                     /translation="VDVLLIDDIQFLADKEQTQIEFFHTFNDLHQANKQIVISSDRPP
                     REIKALEDRLRSRFEWGLITDINDPDYETRVAILKKKAERDNKLITEDVYEFIAKNIK
                     SNIRELEGALTRVIAYSSLVDRTIDIALCEEALAEILDKTLVRKIDSNYIREIVCQHF
                     EVTEQELDSPKRTRSIAYPRQVAMYLIREMTDLSLPKIGEIFGNRDHSTVVHSCDKIT
                     KEKETKESTKELLETLKEML"
BASE COUNT         3272 a         2442 c         1916 g         4353 t
ORIGIN      
        1 atcctaaaaa atcacctata ggggttcgta ctgctgcaac taccacaact tttttcaaag
       61 ctctacctcc tttataattt ttcaacttgt ctatgcttgt tttctaccat tcttgtaagc
      121 gggtaggtct ttggtaaaaa acaaacctga ctcctttgcc aggatcatta ctagactttt
      181 tataaaaagc tgccttatgg gcagcaaatt tttttgtttt tttgctaaag ctggcccttg
      241 agctcttcta gctagttttt ctgagtaaaa cattgtctga taaaagaagg cttcttatag
      301 ctaattagat cttctttttt tcttagaatt gtgtactaat tataacttat aatgattatt
      361 atgtatagga aaaatgaata aaactcctaa tttgcaaaaa aataaggagc ctaagctccc
      421 tatatctcta ttacttttct atattcttct tgaaatttcg ccaaaatctt tttttccatt
      481 ctggatatgg tcatttgaga aacagataat tccttggcaa caacaatctg ggttttttcc
      541 tcaaaaaagc ggtcttctat aatttttcgc tccatgggat ttaatttatc cataataaaa
      601 tggataaact cctcattgtt tattgcttca aaatttttat cttcttctcc cacaatatcc
      661 ataaggcgaa cgtccctatc ttctccacct gtttcaagtt caatatctag acttcttggt
      721 gtatagacct ttgatgcttc catggcttct attacagcct catcgcttac actaagatat
      781 tctgcaatat cattaactgt aggtggtccc ccttggcttt tatagagttg atctctagcc
      841 agggtgattt ttttagatag ttcctgaatt cgtcttggta cacgtatggt ccaaccctta
      901 tccctaaaat gtttttttag ttctcccaca attgtagggg tagcaaagct agaaaactca
      961 aagcccttgt ctacatcaaa acgttgaaca gcataaagca gacccaggga agcaacctga
     1021 aagaggtctt cgtggtcaat tcccttatta acgtattttt tcgtcaggat ttccgcgata
     1081 tatatatgcc ttgtataaag ctcatcgcgt atttcattat taggactcgc tttgaatagg
     1141 cgaaataatt ctttcgtttc tacgtcttta tatgacattt ttcacctaca ctatcttttt
     1201 tttatcatgt gaatggttgt ccctttttcc tcttctgatt caacaatcat ctcatccatt
     1261 aaagaatcaa tgatataaag gccaagaccg ctccccatat aatcctctgg ggtagggggg
     1321 tttattttct caagaacaaa gcctcctcct tgatcggaga cgctaatttt cataaaatct
     1381 ttgcctattt caataataat atcgatgctc ttagaacctc ttccatgaag aaccacatta
     1441 ttacaagctt ccccaacagc caagcgtata tcttctactt tttctatatc aaagccatga
     1501 atacccgcca cagatgaggc agctaggcgt gcggaaatta catattcttt tttattgggt
     1561 atattcaatt taaatatttc catttctcct ccttcaacta gcatcatcat acccatcaat
     1621 aaggaaatta ttcttcacgg acgcgtataa ccttatcaag acctgtgatg cgaaagagtt
     1681 taagaacact agattgaggt tttgccagta ccaattctag gtcgtttttc tttagacgtt
     1741 ttacaatgcc aattagcatt ccaaggcctg tactgtctat ataatcaagc tcctcggcat
     1801 taatgatcat gcttttttct ttttcttcta ggattgcttc tagtttttct ttcacctcag
     1861 gggaggtgtg aatatcaact tctcccatga tttttacttc ccagcaatct ccccgttcgg
     1921 aaaacaaata tctaatcttc actgccatct tcttcctcct ccgtctctct agccttagca
     1981 aatccgacca cttctacttc gtcttcaaac ctcattaggc taactccaga agtgtttctt
     2041 cccatttttg atacatccat agcccggatg cgtatcatgc tgccttggga attaataaga
     2101 aagagttcat cttcttctgt aaccatactt actcccacca catgacccgt tttatccgat
     2161 agtttatagt tgatcattcc tttaccgcct cttccttggc tgcggtaatt ggtaagttct
     2221 gttctttttc cataaccctt tgatgtgatg gttaaaatgt ctccttcttt ttcatttaca
     2281 atttctgtgg agactacttc gtcttctttt tcaagttgaa tactctttac tccccttgta
     2341 tttctattta aaattctcat ttctgatgat gggaatcgaa tggattttcc aagctttgtc
     2401 gctacaaaaa catcagaatc ttcatcaaga atgcgtacat gaattacttc atcgccctca
     2461 tctaagcgaa tcacttttat tccgccctga cgattgcttt caatgttttc taggagcatt
     2521 ttattgataa cgccttcttt tgttgctagg acaatgcgtc tatcatccat ttcattaaca
     2581 gggataatac tggttacctt ctcgccttca tcaagttgca ataaattgat aatgggtgtt
     2641 cccctacttt gtctacttga ttcaggtatg cgataggctt ttaggcgaaa tagttttccc
     2701 agattggtaa aatataataa atcactatgg gtagaagtgg taaatatatc atagatcaca
     2761 tcttcatccc gagtggtaag tcctattatt cctcttcctc ccctattttg ggattggtag
     2821 acatcttggg ctactctttt cacataacct ctttgggtaa gagtaattac catttttctt
     2881 tcttcgatta gatcttcatc ttctaattct tcataactat gctcgatact tgttcttctt
     2941 tcatctccat agcgttcttt gatatcttct agactttctt taataatatc cataagaaga
     3001 ctttcatcac ctagaatact aagataataa gcaatgtctt tttgtaatcc tgtaagctca
     3061 tcttcaatct tcttatactc aagaccctga agacgtctta gctgcatact aagaatacta
     3121 tttgcttgga tttctgtaaa atcaaagctt tctattagct tttctctcgc atcatcataa
     3181 gatgagcgaa taatgcgaat aatctcatct atattttcaa gggcctttaa tagtccctct
     3241 agtatatgag ccctgtccct tgcttttttt agatcatatt taattcggcg agtttctaca
     3301 tctttttgat gtagaatata ttgttctatt aaacccttta gggtaaagac cttgggcatt
     3361 ttgtcttgga gggcaataaa aatcatacta aagacagatt ctagagtaga gtttttatag
     3421 agttgattta aaataacatt ggcattggca tccctgcgta cttcaagaac aatgcgaatc
     3481 cccttacgac tggactcatc tctcatttct gtaatgccat ctattctttt tgtccttacc
     3541 aggtctgcta tactttctag aagttttgtt ttgtttacca tatagggaat ctcagtaata
     3601 acgatacgag agcgtccctt gccaaactct tctacagaag ccttagagcg aatcctcacc
     3661 ctgcttcgac ccgttcgata agcttgacgg atggattctt ttcccataat tgttgctcct
     3721 gtgggaaaat ccggaccttt aataatctcc ataaggtctt ctgtatcaca ctcaggataa
     3781 tcaatggcat agatagtacc atcgataatt tctcttaaat tatgaggggg gatgctggtg
     3841 gtcattccta cagcgattcc tgttgagcca tttactagca aattgggaaa acggctggga
     3901 agaacccagg gctgcattaa agactcatca tagtttggat aaaaatctac tgtttcttta
     3961 tccaaatcac gtagcaattc atcggctata ggagaaagcc ttgcttcagt ataacgcatt
     4021 gctgctgccc catctccatc aatggatcca aagtttcctt ggccctcaac aagtaaatat
     4081 cttgtagaaa actcctgggc caaacgcacc atggcattat aaatagcaga gtctccatgg
     4141 gggtgatatt taccaatcac atcccctacg atccttgctg attttttaaa gcttttatcg
     4201 tgggtatatt tttgttcata cattgagtaa agaatacgtc tatgcacggg ctttaagcca
     4261 tccctaacat cgggcaaggc tcgggagaca atcacactca tagagtaatc aatataggat
     4321 ttttgcatct ccctgcttat atctctttca ataatttgtt ctttcacatt atctcctata
     4381 catccaggtt cgttacgtta acaccatgtt cttggataaa ttgacggcgc ggctcaacct
     4441 ggtctcccat aagggtagta aagacttgat cggcttcaat cgcatcatca atatttactc
     4501 gaataagaat tcgattctct ggatccattg tcgtggtcca cagctgttca gcattcattt
     4561 caccaagacc tttatagcgc tgaatatggc tgtttctgcc cattttttca attaagatct
     4621 gaagttcatt atccgtatag gcatattcta tcttgtttcc cctttttact gaataaagag
     4681 ggggctgtgc caaataaata tgtccgtttt ctataagggg tgttagatat cggtaaaaga
     4741 atgttaaaag tagggtccgg atatgggctc cgtcgacatc ggcgtcggtc ataataataa
     4801 ttttatgata acgcagctta tctatttcaa actcatcccc aattccaacc ccaaaggctg
     4861 taaccatatc tctaatttct ctcgagttaa gagcgcggga aaagtttgct ttttctacat
     4921 ttagtatttt tccccgaaga ggaaggatag cctgatagct tctttcccga ccgagttttg
     4981 ctgttcctcc agctgagtca ccctcaacaa tataaatttc gctaagtact gggtcttttt
     5041 cttgacaatc agagagtttt ccgggaagag aaaaaccatc tagtagtcct tttcttcgtg
     5101 ttaaatctct aacctttctt gcagcaatcc tggcctttga ggagcggata catttttcca
     5161 taataaactt ggcttcctgg ggctcttcca taagaaagtt tgacaggatt tcactacaaa
     5221 ctgaatccgt tattcctcgg agttcactat tgccaagttt tgtcttggtc tgcccttcaa
     5281 actgaggctg actcactttt acagacaaaa tagccgtaag tccttctcta acatcatctc
     5341 caataaggtt atcttcgttt tctttgataa aaccgttttt tcttccatat tcgtttatca
     5401 ctcgggtaag ggcggctctt aaacctgata agtgggttcc cccctcttgg gtattaatat
     5461 tattggcaaa ggtatggatg ttttctgtat aggtatcact atattgtagg gcgatttcta
     5521 ctacaactcc atctcttact tgctcaccat ataaaatctt cctatgcaag gctgttttat
     5581 ttcgattaat atatttaaca aactcagcaa tgcccccttc ataaaagaaa tgaacttctt
     5641 tttcatgaat atgatcgata aaatcaattt tgattccttt atttagaaag gccatctctc
     5701 taaatctgtt ttccaaaatt ttattatcga attctatttc ttcaaaaatt tcttcatcgg
     5761 gccagaaaat ggttcgggtt ccctgctctg atgtttctcc tattatttca aggggtcttg
     5821 taataattcc ccgggcaaat tctgtgcggt gtatcttacc ctcgcggtaa acttctacaa
     5881 tcatgttttt acttagggca tttaccacgg cagctcctac accatggagt cctcccgaaa
     5941 ctttatattc ctcattatta aattttccac cagcatgaag tacggtgtga attacttcaa
     6001 cagcagaaat attgagttta gggtgaagat ctgtgggcat tccacgacca ttatccttaa
     6061 cctctacgct actatcttta tgaatcacaa tagaaattga atcagcaaag ccattgatgg
     6121 cttcatcaat gctattgtcg ataatttcat atattaaatg gtgaagtcca cgctgtcctg
     6181 ttgttccaat atacatacca ggtctggttc taacaggttc tagtccttca agaacttgta
     6241 ttttgtctgc tgaatatctt tcactcatat tttctcccct ctacaattgt cgttttatta
     6301 aaatctttta tcttagataa atccaagcca tcaaggtccg ttccactaat aattacttga
     6361 taatcttgga gaagttccaa tagttgatga cgcctaaatc ggtctaattc actaaagaca
     6421 tcgtccagta gtataagagg ttttttttca agctgttttg ttataaaggg taaaagccca
     6481 aaaaaaatac tgatagaacc gatacgcact tggccttgac tggcaaatct acgagcatcc
     6541 ctcttatcta tttcaatata catatcatca ctctggggac caaagctggt ataacccaga
     6601 gaaaggtctt tttttctatc ttcgcgatag cgctttctgg ggtcctctac accttgaaaa
     6661 gaactcttat aacttaagca gagttcttct tttcctccag aaataaattc gtgtactatt
     6721 ttgctttgtg catttaggat ttcaacaaaa agtcctcggg ccttatatat ctttttccca
     6781 tatagggcta atttttcttc atatacatcc atttgtaggg gatcaaagtt ttcttttaaa
     6841 aggatattgc gtgagcgtag tagcctttga tactgaatca ggctataaaa atatgctggg
     6901 cttaccaggg atatttctct atcaataaac tttcttctgt aacttggccc ttctctaaga
     6961 actttaagat gttctggaga aaaaagaacc atgggaaatc gcccaaaaag ttcacttctt
     7021 ctttctatct caatattatt tactgaaatt accggttttt tatccttgtg ggtataaacg
     7081 aattcaaagt cttctgaacc ttgagaaaaa ttaagttcta ccctaaaggg gtcttttctt
     7141 tcatgaaaag cctctctagg actccgtgca cgatagacct tccccataca ggcaagatag
     7201 atagcttcta gaagatttgt ttttccctgg gcattgtctc ctaagataaa ttcaaagtcc
     7261 gaagaaaaat agaactcttc atcaacaaag ttacggtaat taattaatcg tgcatagttt
     7321 attttcataa attctcctgt atacatttta gcacatttta gcaatttatt caaaccaccg
     7381 tagggggcta cctaatataa aaaaaaggcc ctatttcgcc taatttgctt cctctttttg
     7441 ctttatgcct tcttgagcat aagtgaaaaa agctaaaaat attaagtttt acataaataa
     7501 taaacaaaaa gcaaaaacaa gacctttttt ttctataggc agaagcctgt ctgcttattg
     7561 tcttatttgc ttgctttctt gcaaaagatc caatctttta ttttatattt aaggatatgg
     7621 atttatctta gtttatcata ttgaggtttt tttccaaaac gataaaaaac acccttgctc
     7681 tttagtaagg ctttgcttgt gtctttatat ggtgcacttt tcttttttaa tttgatagtc
     7741 tagggctgta tctttttatc aggtctagat agtccttatt cttccataaa aattcatatt
     7801 ttttgttttt ttctatttcc tttattaagg aaagcaaaaa aaactccccc atcctgcctg
     7861 cttctttctt tgtttttata tttctatata ggggggaatg attaatatcc cattttctat
     7921 ccattgcctc aaatatgttt tttagcaagg ctagggattt ttcttggtcc ttagtaaaaa
     7981 ccgctaattc gaaattagca atataggcac tgcagtccca aagatcatag agccttgctg
     8041 ttttttcaaa aaccttggct atatagtctg cttcttctat tttcttttct ttttgattta
     8101 cttgggctaa ttttaataaa agaccctgga tttcgttaat tttttctagc aagccttctt
     8161 ctaggagttt catggcttca atattttttt tctgttttat atatagactc gtctgaatct
     8221 gtcctttatc aattccttgc tcaggcaaag agtcaattag tttttgtgct ttttcgtact
     8281 ctgctctttt tatatatttt gaaatcattt tagaaatagc ttgatcacga atcttactat
     8341 catcactgtg agccaatcgc ttatataaac tttcaatctg tctaccatac ttttcactat
     8401 ctttgttttc aaagactaga agagatcctt ctaaagttaa ggcaatcgtg tagattagtt
     8461 tagggctagt aggatattct tttatttttt cttctgctag agagtatcct tcttcaaaac
     8521 cttttttttg gatggtagag cttagctcat tgataaatct tcctatttcc tcctctgata
     8581 actcttcttt aaatgataag agggtattta ggtcggtttt aagtaatcta gccaggctgg
     8641 gtaagaggct aatgtctgga tatgaaactc ccttttccca cttgtttact gctggagtag
     8701 agacacccaa atattctgct actttttttt gggttaatcc tagctctagg cgttttttcc
     8761 ttattatttt atttattctc attttttacg cccctttctt tttattaagg atctacacct
     8821 ttattatatt gattttactc tgtctttaca attgagcttt tcttagtttt ttctgggata
     8881 agttaaccag tggttaattt gacccaaatg ctttttcgtc cttttataag cttcataatt
     8941 ctaataaata ataaaagccc agaaattaag cttctgagct tttattatct attggttttt
     9001 tatcgtccca gctcccttgc cgcatcttca tctaaaagaa cgactatatt gggatgaagc
     9061 tgtaaaacac tggcaggaag atctgtggtt atgggtccct ccaaggttcc ttttattgcc
     9121 ctggccttat ttgcgccatt ggcaattagg aggatttttt ttgcttccat aatacttcga
     9181 ataccaaggg ttatggcctg gcttggtacc tcttctattt tattgaaaaa acgagagttg
     9241 tctttaatgg tgttttcctt tagctccact acatgggttc ttgtggaaaa agaggttccc
     9301 ggttcattaa atccaatatg tccattggtt ccaattccca gaagttgaat atcaagtccc
     9361 cccatgtctt ttatcttttt atcatataaa aggcattctt tttctatatc ctctgcattt
     9421 ccatcgggga taaaccagtg tttttcatct atatctactt tattaaaaag tttattttcc
     9481 ataaaagcac gataacttcc tggatttttt ggatcaattt ttatatactc atcaagatta
     9541 aaggttttta tctttttaaa actaatcagt ccctcatcat acaaacgaat tagtctttta
     9601 taagtaagct taggagtgct tcctgtagca agaccaagta cggaatctgg tttttttagc
     9661 atctgtactg caagaattct tgtagctgct ctactcattt cttcataatt tttgtatatt
     9721 tttatttcca ttggttttcc tttatttttc ttctaaaaca acttcaaccc tttcgtgtat
     9781 gccctcttta gcaataataa cttccactaa atcaccagaa aaaatctttt ttcctctttg
     9841 ggtgcaagtt gtaccattaa gataaacaaa accatcgcgg attaaatgtt ttgccaagcc
     9901 cccactatct acaatagcag caaacttaag tagctggtct aaacgtatat ggtcggtctt
     9961 tatttttact atcattgggg ggtccttatg gggactacat aatattcata gtcgtcttta
    10021 tctttttgtg ttattttaca gggtttatga gaacctccca tagcaaatac catactgtct
    10081 tcttccatta ctcgtaaggc tcttagaaga taattgggat taaagtttat tactaggggt
    10141 tctccatgat ttacggtaga aatactttct tttgcaagac ccagttcgga agaacttgat
    10201 atatctactt ggcgatcgct aaaatcaaaa ttaacaatat aacttttaga gcgattggca
    10261 agaagggcaa cccggtccag tgctcgataa aattcgtcaa taggaatttc tacctttgta
    10321 tcaaaacctg tatcgatgac tctttcataa tttatatagt tgccttctaa aactttagag
    10381 ataattgtga ttccatcaaa attaaaaaca acatggttgg aagaaacaag tatttccatt
    10441 tcttcttctc ctgtgcctat aattttttcg atttcactta gatttctacc aggtataacc
    10501 acctggcagg gatttccttc taaaatggga acggttttta tggcaatggt aaatccatcc
    10561 acagaaacca ttttcatttt tccgtctttt acttcaacta gacttcctgt taatatgggg
    10621 cgggtttcat cttgtcctac ggcaaatttt gtcattttaa caagagaaga aaaagctcct
    10681 tgttctatct ttatagaact tccttcattg atttgaggta tttgaggata gttttcttga
    10741 tccatcatac ttagtctata tctacttgat tgacaaacaa gaaataaact ttcatctttt
    10801 atttccatgg tgattggctc accagggagt tttcttataa tatcactaaa aagcgaagaa
    10861 tcaattagta cacttccttc tttttctaca aaggcaggta cctgcataat aatagataag
    10921 ctcagatcgt gacttttcaa tataatcata ttatctttag cttctatatg tatccctctt
    10981 aatatttcca tagtcgtacg cagatttgtt gctttactaa ctatgcttaa ggcagagact
    11041 agttctcttt gatttagggt gaacttcatt actcctccat atctataata tatagtcgta
    11101 gtagtagggg ctgttgatat gttaacaagt aggctaagcc tttgcaagca cgggataagt
    11161 ggagtggaaa agcctgtgta gaaaaaaagc ataagtttga ctttatccac agcttatttt
    11221 atccacagaa tttctcactt tatccccagg cttcttggga taaattataa catttctttt
    11281 aaggtctcaa gaagctcttt tgttgattct tttgtttctt tttcttttgt tattttatca
    11341 caggaatgca caactgtgga gtggtctcta ttgccaaaaa tttctcctat ctttggaaga
    11401 gataaatcag tcatttctct tataagatac atggcaactt gtcttggata agcaatggat
    11461 ctggttcttt taggagaatc aagttcttgt tctgtgactt caaaatgctg acaaacgatt
    11521 tctctaatat agttactatc aatttttcta accaaagtct tatcaaggat ttctgccaag
    11581 gcttcttcac aaagggctat atcaatggtt cgatctacca gtgaggagta ggcaataacc
    11641 cgggttaagg ccccttcaag ctcacgaata ttgcttttta tatttttggc tataaattca
    11701 tatacatctt cagtgattag tttattgtct ctttcggctt tcttcttaag gatggccact
    11761 ctggtttcat aatcaggatc attaatatct gtaatcagtc cccactcaaa cctagaacgc
    11821 aatctatctt ctaaggcttt gatttctcga gggggccgat ctgaacttat aacaatctgt
    11881 ttgttggcct ggtgtaagtc attaaaggta tggaaaaatt caatctgggt ttgttcttta
    11941 tcagctaaga attgtatatc atctataagg agtacatcta cat
//
LOCUS       DASRNA010000099         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_251209,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000099 DASRNA010000000
VERSION     DASRNA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251209"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..630
                     /locus_tag="VFD08_02710"
     CDS             118..630
                     /locus_tag="VFD08_02710"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZK09959.1"
                     /translation="MNLIDKIRVIEDYPAEGISFKDITTLLSEPDAFYEAVQAMIKSS
                     EDIEFDVILAAEARGFIVGAPMAYALKKGFVPVRKPGKLPGETIRREYALEYGKDFLE
                     IHKDAIKPGDRVLIVDDLLATGGTSSAIKEMVLSMGGQVAGYVYLIELEFLEGRKLLG
                     DYPVKSIIRY"
     gene            640..2766
                     /locus_tag="VFD08_02715"
     CDS             640..2766
                     /locus_tag="VFD08_02715"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZK09960.1"
                     /translation="MDRELYLKRLGEINPHYDDKRIGEAFDFADKAHEGQFRRSGEGF
                     IIHPVEVSLILASLDMDEDTIIAGLLHDVIEDTTYGYERIISLFGKEVGEIVDGVTKI
                     EKISYASKEVQQAENFRKMLLAMSQDLRVIVVKLADRLHNMRTLKYKSEESKKRTANE
                     TLEIYVPIAHRLGIFKLKWELEDLSFYYLNPLEYRDLSNRLDQKRAEREKYIQKIIGE
                     IKELLESENLECEIYGRPKNLYSIYKKMTVKDRAFEEIHDLTAIRVIVKEVSQCYAVL
                     GFIHSRWKPIPGRFKDYIAMPKPNMYQSLHTTLLSENADTFEVQIRTEEMHRVAEYGI
                     AAHWKYKEGQKSISTSSDMHWIEQLMEWQREMDNPTEFINSVKMDLYNTHVYVFTPMG
                     NVIELPDGSTPVDFAYKIHTDIGNHCVGAKVNSRIVPLNYRLKIGDIVEILTSKSSQG
                     PSRDWLSFVQSSKARNKIKNFFKKESQQENIEKGKEALEKNLRKINLTLKEAFVPKVV
                     DSLLDKLSLKSLDHLYAAVGYGGIGFRQILPVIKETLLPEEKELHLGKTRSRNSNTGI
                     IVEGIDDLQVRFAKCCNPLPGDNIIGYITRGSGVTVHKSSCTNFSKGKDEMNRFIEVE
                     WDDSVDSPHSASLKIIGRDRPGLLSDITSLVQNMSYTVTGVSARVNNEQLTRIVISFD
                     VKNVSELEKVSHKLKTVSSVMGVYRL"
     gene            2763..>2976
                     /locus_tag="VFD08_02720"
     CDS             2763..>2976
                     /locus_tag="VFD08_02720"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZK09961.1"
                     /translation="MRAVIQRVKKAKVCVLDECVGEISQGFLVLLGVEKEDTPEDLDY
                     MVKKIHGLRIFSDEQDKMNLNLAAVGG"
BASE COUNT         1029 a          474 c          649 g          824 t
ORIGIN      
        1 cacctatact aatattaatg gtatatgtta ttatgaagta ataagcacga tgaagaaaaa
       61 caatctagaa tcatcaccga cttattgccg tattatagaa attagggagg atatgtaatg
      121 aatctaattg ataagataag agtaatcgaa gattacccag ctgagggtat cagttttaaa
      181 gatataacta ccttgcttag cgaacctgat gccttttatg aggctgtgca agctatgatt
      241 aaaagcagtg aggatattga atttgatgtg attttagccg ctgaagccag aggttttatt
      301 gtgggtgctc ctatggctta tgcccttaaa aagggctttg tacccgtaag aaaaccagga
      361 aagctgcccg gtgaaacaat aagaagagaa tatgcactag aatacggaaa ggactttcta
      421 gaaattcata aagatgccat taagcccggt gacagagttc ttattgttga tgatttactt
      481 gccacagggg gaacttcttc ggcaattaaa gaaatggttc tttctatggg gggacaagta
      541 gcaggatatg tctatcttat agaactagaa tttttagaag gcaggaaact gcttggagat
      601 tacccagtaa aaagtataat tcgttattaa aggaaaatta tggatagaga actctattta
      661 aaacgattag gggaaattaa tccccactat gatgacaaaa gaataggcga ggcttttgat
      721 tttgctgata aggcccacga aggccaattt aggagatcag gcgagggttt tatcatccac
      781 cccgtcgagg ttagccttat tttagcttct ctagatatgg atgaagacac cataatagca
      841 gggcttcttc atgatgtcat tgaagatacg acctatggat acgaaagaat tatatcttta
      901 tttggaaaag aagtaggtga aatcgttgat ggagttacta aaattgaaaa gataagctat
      961 gccagtaaag aggtccagca agccgaaaac tttaggaaaa tgctgctagc tatgagtcaa
     1021 gatttacgag tcatagtagt gaagcttgct gatcgtttac acaatatgcg gaccttaaaa
     1081 tataagagtg aagagtcaaa aaagcgaaca gctaacgaaa ctcttgagat ctatgtaccc
     1141 attgcccacc gtctaggtat ttttaagtta aagtgggaac tggaggattt atcgttttat
     1201 tatttaaatc cattggagta tagagactta tctaatcggc tagatcaaaa acgagctgag
     1261 agagaaaaat atattcagaa aattataggt gaaataaaag agcttttaga aagcgaaaac
     1321 ctagaatgtg aaatatatgg acgccctaaa aacctctaca gcatctataa aaaaatgaca
     1381 gtaaaagatc gtgcctttga agaaattcac gaccttacag caattcgagt cattgtaaaa
     1441 gaggtgtctc agtgctatgc agtccttgga tttatccata gtagatggaa accaatccca
     1501 ggaagattta aagactatat tgctatgcca aaaccaaata tgtaccagtc ccttcatact
     1561 acactacttt ctgaaaatgc cgatactttt gaggtacaga ttcgtacaga agaaatgcat
     1621 cgagtagctg agtatggtat tgctgcccac tggaaataca aagaaggaca aaagtctatt
     1681 tcaactagta gtgatatgca ctggattgaa cagcttatgg agtggcaaag agaaatggat
     1741 aaccccacgg aatttatcaa ttccgttaag atggatttat acaatactca tgtgtatgtt
     1801 tttaccccaa tgggcaatgt aatagaactt cccgatggca gtacaccggt agactttgcc
     1861 tataagattc atacagatat tggaaatcat tgtgtaggag caaaggttaa tagtcgcatt
     1921 gtgccactta actatcgatt aaaaatcgga gatattgttg aaattttaac atcgaaaagc
     1981 tcccaaggac ccagtagaga ttggctcagc tttgtccaat cttctaaagc aagaaataag
     2041 atcaaaaact ttttcaaaaa agaaagtcaa caagagaata ttgaaaaggg caaggaggcc
     2101 ttggaaaaaa accttcgtaa aataaatctt accttaaagg aagcttttgt tcccaaggtt
     2161 gtagatagcc ttcttgataa acttagccta aaaagcctag accacctcta tgccgctgtt
     2221 ggttatgggg gaatcggttt tcgacaaata ttacccgtta ttaaggaaac cctccttccg
     2281 gaagaaaaag agctacattt aggaaaaacc cgatcaagaa actctaatac tgggattatt
     2341 gttgagggaa ttgatgatct acaagtgcga tttgccaaat gttgtaatcc tttacccgga
     2401 gacaacatta ttggctatat tactagggga agcggagtga cggttcataa gagttcttgt
     2461 acaaatttca gtaaaggaaa agatgaaatg aaccgcttca ttgaagtgga gtgggatgat
     2521 tctgttgatt ctccccattc ggccagccta aagattattg gaagagatcg ccctgggctt
     2581 ttgagtgata tcacttctct ggtgcaaaat atgagctata ccgttactgg ggtgagtgcc
     2641 agggtcaata atgaacaact tactagaatt gttataagct ttgatgttaa aaatgtgagt
     2701 gaacttgaaa aagtttcaca taaacttaaa actgtgtcat cggttatggg ggtttatcgt
     2761 ttatgagagc ggtaatacaa agagttaaaa aagcaaaagt atgtgttttg gatgaatgcg
     2821 tgggagagat atcccaggga tttctagtcc ttctgggggt ggaaaaggaa gatacaccag
     2881 aagatctaga ctatatggta aaaaaaatac atggacttag aatttttagc gatgaacaag
     2941 ataagatgaa tcttaaccta gctgcagtag gaggag
//
LOCUS       DASRNA010000100         6968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_335990,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000100 DASRNA010000000
VERSION     DASRNA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335990"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1188)
                     /locus_tag="VFD08_02725"
     CDS             complement(373..1188)
                     /locus_tag="VFD08_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZK09962.1"
                     /translation="MKENKEKQLILAEFSPSGSTNEIGTYLIKKLGEKTGMPWQHRSF
                     TKPSERKERLFFDKDSWVVFAMPVYAGRLPSLLKPYLSHMQGRGAKVFLVSVYGNRHY
                     DDALREMEEFSIAAGMTVVGGAAIVARHVFSEIIARGRPDKKDFEIIDRYIPSIIEAF
                     TAGNSFVIPGEGELKYYQPTGIQGEKTNIVKVKPSTKAGCIACQKCVESCPLGSIDSL
                     DPRVVSGKCMKCCACLLACPLGLKHFDDRDFQSHLLSLEENFIKRREPEFFLP"
     gene            complement(1508..2215)
                     /locus_tag="VFD08_02730"
     CDS             complement(1508..2215)
                     /locus_tag="VFD08_02730"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HZK09963.1"
                     /translation="MAKKYKREILIILGLILICCGAFIVESILAGRSSEEVLDKIKQS
                     EVVAEKIDRIKATEEVEEAALVIPIDFDAAWQINEDAIAWIKIPNTTVDYPILQSPDE
                     DNYYSDHDIEKIQHWIPGSIYINLNNSPDLSDRNTIIYGHNVHVLSGDGTGTMFGDLV
                     KYYEEEFFQKNKEIIIYTPQNKLSYEIFAAVTYNNKSIPDLYDLDTREGHQDFLQSIY
                     NTNMKTDLYSDKEVGGR"
     gene            2514..2984
                     /locus_tag="VFD08_02735"
     CDS             2514..2984
                     /locus_tag="VFD08_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK09964.1"
                     /translation="MHSRDVFIELVNEADKKAQIVKEVLFDLPKWFALPESTKDYIEQ
                     SKKLPLLVARNNKLILGFITLKETSEAVCEIHCMGVKEEHHRKGIGKKIYQAFEEVAK
                     KKYEFVQVKTLDEGLCKEYDQTVAFYKKLGFKKMEVFPRLWDEQNPCLIMIKKI"
     gene            complement(3173..4240)
                     /locus_tag="VFD08_02740"
     CDS             complement(3173..4240)
                     /locus_tag="VFD08_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HZK09965.1"
                     /translation="MKRIDERLEGLKTYWKSSKAESKKAERNRLAVLIDMLYYRFKYG
                     FSALDYQVFGFAHTRDKEARLSYFGALDWANANYFLNLPKDVEFDFFNKIDVYKLLKD
                     YYAREVIILDEVGIPKLQEFMEKNPIFFAKQASNYGGFGIEKLYSKDFHSAIDLYDYC
                     KKHKLELVEELIKQHPDVNKLYPLSVNSLRITTIVDDNKKVHFIPSILRMGGENMEID
                     NVSSGGVYVQVDRSGKIVTHGFKEDGEFSLDGGHLVECHPSTGTRFLNYQLPNTDEAY
                     ALAEELALKVPGYRYIGWDIALTETGVDLIELNTYAAYDMSQCYYQNASKRGSYDLMA
                     KLVNVDFRSLEDRYKKVRTNK"
     gene            complement(4254..5273)
                     /locus_tag="VFD08_02745"
     CDS             complement(4254..5273)
                     /locus_tag="VFD08_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HZK09966.1"
                     /translation="MLSKIKKGLQMDWPQIYYVVNHMHKRSGRSHRDLFLDMMKSYKE
                     EGYTWLNYLTFAFDEITDPLIRETYLSEYRDNTKIIEACNTKENMDIFEDKGRFNRRF
                     KAFLGRETLDLREESYTDFREALLKHEALFFKTPCDCGGDNILRLETESIFDPKKLYN
                     SLIKNNQVVVEEEIKQDPKMSELSLKSINTLRVGTATNPEGKVSVAYMTLRFNTTDTH
                     FDNSSLGGAFTLLSDDGVIKYPGYINYPKEKSYTIHPATGTHLIGFELPQIQEVKNLV
                     CESAKILPQSRYIGWDVAITPSGPVLVEGNSFPSVELYQARIHMEDGRGKAYLMEELL
                     GLSLR"
     gene            complement(5407..6771)
                     /locus_tag="VFD08_02750"
     CDS             complement(5407..6771)
                     /locus_tag="VFD08_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HZK09967.1"
                     /translation="MKKLRFYLAITLARLIALILKLTGHNATHTPGALALKLCPDFLS
                     HLAKPKTVIAITGTNGKTTSSNLIADLYKKLGFSFAHNSYGSNIKEGVITTLLDASTF
                     FGGPKLELCILEVDERASRIIFPEMAPDYLLVTNLFRESYLRNAHGEFVFRVLKDHIP
                     SHTHLILNSDDLISSRLGKDNPKTFYSISPLAEEKSQKGNLIEDMPLCPLCNQPMVYD
                     FRRHHHIGRAHCSSCAFTNYKADFVLEDVGESFTINHKGRSEEYPILGENILDHYAEL
                     CALSLMRTLKFSHKELSQAIEGLEIVKSRKDISQVKEKTLYRMLAKGMNPIAISRALE
                     IVLSQEGKKALVLLNDTSRKKDSHKENTAWLYDTDFEQLKSQDVDQILVGGYRAEDYY
                     LRLLFAGIAEEKIILCEEKADVADKINLERVDFIGVLYELYSEPISRQIINKITKRLN
                     EHPL"
BASE COUNT         2054 a         1393 c         1212 g         2309 t
ORIGIN      
        1 gaggatttta agagtttatt gtaatattct tttttgcttg attggctagc ttaaaatctg
       61 caattccaaa ctaagaagaa ctatcctctt aaattatcta ccacttcttt tttcatcaaa
      121 cgcttattaa aaaaataaat agaaataaat atgcttgata gagaaaatgt aagaatacta
      181 actaggatcc ctataaagtt cgtctcccta agaagacccc ctagactatg taaatactta
      241 gctctaagta tttattataa aaatctaaga caagatcgca actgaccttt tttctttagg
      301 gataaatcat acctacaaat ataatcaggc ctctagccaa gctctctata aacctttaaa
      361 agagatcacc tactatggta gaaaaaactc tggctctctg cgcttgataa agttttcttc
      421 taaagaaaga aggtggctct gaaagtctct atcatcaaaa tgcttaaggc ctagtggaca
      481 ggctagtaga caggcacagc atttcatgca ttttccagat acaactcttg gatctagact
      541 atctattgaa cccagaggac aactttccac acatttttgg caagcaatac aaccagcttt
      601 tgttgaaggt ttaactttca ctatatttgt cttttcaccc tggatcccgg tgggctgata
      661 gtacttcagt tccccttcac caggaattac aaaggaattt ccagcagtaa aggcctcaat
      721 aattgaaggg atataacgat caatgatttc aaagtctttt ttatcaggcc tcccccttgc
      781 aataatttca gagaatacat ggcgagctac tattgctgcg cctcctacca ctgtcattcc
      841 agctgctata ctgaactctt ccatctcccg cagggcatca tcatagtggc ggtttccata
      901 gacacttact aaaaacacct tggcacccct gccctgcata tgcgataaat atggttttaa
      961 taaacttgga agacgacccg catagacagg catggcaaaa acaacccagg agtccttatc
     1021 aaaaaaaagc ctttcttttc gctctgaagg cttggtaaag gatcgatgtt gccaaggcat
     1081 tcctgttttt tcgccgagtt tttttataag ataggtccct atttcatttg tactcccaga
     1141 ggggctaaac tctgctagaa tcaattgttt ttctttgttt tctttcatct tgattagagt
     1201 cctttctctt ttttcagtta aaagtactat taggtttttt tggacctagg tcaataagct
     1261 atagagataa agatttgtta cccataccgc atttatttga accagtcgcc aggctcccaa
     1321 aggctaatga gtacaaaaat atatatttta gggggtttct ttactttttt aaccactcac
     1381 cctaaagtaa aaaagaaccc atatataagt tatcctttct tagccttact ctacaagcac
     1441 tgcaagaaga agatacctat aatcataatt tagatcccta gtacaggttg ataaagtaat
     1501 aatcctatca tctgcccccc acctctttat cagaataaag atctgttttc atatttgtat
     1561 tataaattga ctggagaaag tcttgatgac cttccctcgt atcaagatca taaagatcgg
     1621 ggattgactt attattatag gtaactgctg caaatatttc gtagctaagc ttgttctgtg
     1681 gggtgtagat aataatttct ttattcttct ggaagaactc ctcctcataa tatttaacta
     1741 aatcaccaaa catagtccct gtcccatccc cacttaatac atgaacattg tggccataaa
     1801 taatcgtatt tctatcactt agatctggac tgttatttaa attaatgtag atcgatcctg
     1861 gaatccagtg ctgaattttt tcaatatcat ggtccgaata gtagttgtct tcatctggac
     1921 tttgaagtat aggataatcc actgtggtat tgggaatctt aatccaggca atcgcgtcct
     1981 cgttgatttg ccaggctgca tcaaaatcaa tgggtataac aagagcggcc tcttccactt
     2041 cttctgtggc ctttattctg tctatttttt ctgctactac ttctgattgc tttatcttat
     2101 ccaggacttc ttcagaactg cggcctgcta atatggactc tactataaat gccccgcagc
     2161 atattaggat aagacctaaa ataattagaa tttctctttt gtattttttt gccatatgcc
     2221 ttccccttta tcacctacat caattatagc aaagcttagc tgctatttat tataaatctg
     2281 gtatttaata aaaacgatgg aatcatttgt tgtaagagca ttaaatatta tgacccgccc
     2341 atataaaaac attagggggg cttgctagca gggagttgaa aatcaaccat ttcacttctt
     2401 atctaaatcc aaaagatcct ttttatttat cattgccttt gcttatgcta gtataaaagt
     2461 gttgggggtg atagtggaaa taaccgccaa attaattatc aggaggtaat tagatgcata
     2521 gcagagacgt ttttattgaa ctggtaaatg aagctgataa gaaagcacaa atagtaaaag
     2581 aggtcctctt tgatttaccc aaatggtttg ctttgccaga aagcacaaaa gactatatcg
     2641 agcagtctaa aaagctacct ctactagtag ctagaaacaa taagcttatc cttggattta
     2701 tcacgttaaa agaaactagt gaggcagtct gcgagataca ctgtatggga gttaaagaag
     2761 agcatcatag aaaaggaatc ggtaaaaaga tttaccaggc ctttgaagaa gtagcaaaga
     2821 aaaagtatga atttgttcaa gtaaaaactc ttgatgaggg tctttgtaaa gaatacgacc
     2881 aaacagttgc cttttataaa aaacttggct ttaaaaagat ggaagtcttt ccaagattgt
     2941 gggacgagca gaacccctgc ctcattatga ttaaaaagat ttagccctca gcttcccaat
     3001 accgtctgcc tatctctctt aaaaacttct catatttgcc cacttaatat acttgatcca
     3061 tcccatctcc actcttatcc tttgagtttt agccccccca aaagttagga ccatagcaaa
     3121 agacctgtct ttttaaggaa caggtctttt gcttgtttga aaactcttat atttatttgt
     3181 tggttctaac ttttttatag cgatcttcta aagatctaaa atctacattg actagttttg
     3241 ccattaaatc atagctacct cgcttagaag cattttgata atagcactgg ctcatatcgt
     3301 aggctgcata ggtatttagt tcaattagat ctacacctgt ttctgttaag gcaatatccc
     3361 agccgatata gcggtatccg ggaactttaa gggctagttc ctctgctaag gcataggcct
     3421 catcggtatt aggcagctgg tagtttaaaa agcgggttcc tgtagatggg tggcactcaa
     3481 caagatgtcc accatcaagg ctaaactccc catcttcttt gaaaccgtgg gtgacaattt
     3541 ttcctgatcg gtcaacttga acatagactc ccccagagga aacattatca atttccatat
     3601 tttctccccc catgcgtaaa atgctgggaa taaaatgcac ctttttgtta tcatcaacaa
     3661 ttgtggtgat acgtagggag tttacagata ggggatatag cttattgaca tcagggtgct
     3721 gtttgataag ctcttccact agttcgagtt tgtgcttttt acagtagtca taaagatcta
     3781 tcgcggagtg aaaatccttt gaataaagtt tttcaattcc aaatccgcca taattacttg
     3841 cttgtttggc aaagaaaatc gggttttttt ccataaactc ttggagtttt ggaattccta
     3901 cctcatcaag gataataacc tccctagcat agtagtcttt taggagttta taaacatcaa
     3961 tcttgttgaa aaaatcaaac tccacatctt ttggtagatt aagaaagtaa tttgcattgg
     4021 cccagtcaag ggctccaaaa taacttaggc gggcctcttt atctctagta tgggcaaagc
     4081 caaacacctg atagtctaag gcactaaagc cgtatttaaa tcgataataa agcatatcaa
     4141 taagaacagc aagtctatta cgttcggctt tttttgattc tgctttacta gatttccagt
     4201 aggttttaag tccttctaaa cgttcatcaa ttcttttcat tttcattcct tttttatcga
     4261 agagaaagtc ctagtaattc ttccataaga taagcttttc cgcgtccatc ttccatatgt
     4321 atccttgcct gataaagttc tacagaagga aaactatttc cctctacaag tacaggtcca
     4381 gatggggtaa tggcaacatc ccaaccgata tagcggctct ggggcaaaat tttggcactc
     4441 tcacacacaa gattcttgac ctcttggatt tgtgggagct caaagccaat aaggtgagtt
     4501 cctgtggccg gatggattgt ataggatttt tctttgggat agtttatata gccaggatac
     4561 ttaattactc catcatcact taaaagagta aaggctcccc ccaaacttga attgtcaaaa
     4621 tgagtatctg tggtattaaa ccgcaaggtc atataagcaa cagatacttt gccctctggg
     4681 tttgtggcag ttccaaccct aagggtattt attgatttaa gggacaattc actcatcttg
     4741 gggtcttgtt ttatttcttc ttccactaca acttgattgt tttttattag gctattgtaa
     4801 agtttttttg gatcaaatat actttctgtt tcaagccgca aaatattgtc gcctccgcag
     4861 tcacaaggag ttttaaaaaa gagggcttca tgtttaagta gggcttctcg aaagtctgta
     4921 tagctctcct ccctaagatc tagggtctcc cttccaagaa aggctttaaa gcgtcgatta
     4981 aatcttccct tatcttcaaa aatatccata ttttcttttg tgttacaagc ctctataatt
     5041 tttgtgttat ctcgatactc cgacagatag gtctctcgaa taagtgggtc tgttatttca
     5101 tcaaaagcga aggttaggta gtttaaccaa gtataaccct cttccttata agacttcatc
     5161 atatctagaa aaagatccct atgactgcgc ccagatcttt tatgcatatg atttaccaca
     5221 taatagattt gaggccaatc catttgtagt ccctttttta ttttagaaag cattgcccct
     5281 ccccctgaat tacctcttca ttttactttg tgtttttatc atagcttttg tgcttttgaa
     5341 caattgcatg aatgctttct tccctagcaa gagtctcctc taaggcaatt ttctttaaag
     5401 gttcttttat aggggatgtt catttaagcg ctttgtaatt ttatttataa tttgccgaga
     5461 tatgggttct gaataaagct catataagac cccaataaaa tctacccttt caagattgat
     5521 tttatcagca acatctgctt tttcttcgca gagaataatc ttttcctcag ctattccagc
     5581 aaaaagaaga cgcaaataat aatcttctgc cctatatccg ccaacaagga tttggtccac
     5641 atcttggctc tttaactgtt caaaatctgt atcatagagc caggcagtgt tttctttatg
     5701 actgtccttt tttctgctag tgtcattaag aagaactaag gcctttttgc cttcttggga
     5761 cagtacaatt tccagggctc tactaatggc aatagggttc atccccttgg ctaacatgcg
     5821 gtatagggtc ttttccttta cttgagagat atcttttcta cttttaacta tttcaagacc
     5881 ttctatggcc tgagatagtt ctttatggct aaacttaaga gtgcgcatta ggcttagagc
     5941 acaaagctct gcataatgat ctaatatatt ttctcctaag ataggatatt cttcgcttct
     6001 tcctttatgg tttatagtaa aactttcacc tacatcctca agtacaaaat cggctttgta
     6061 attagtaaaa gcacaggaag agcagtgcgc cctgccgata tgatggtgcc ttctaaaatc
     6121 atagaccatg ggctgattgc aaagaggaca aaggggcata tcctctatta agttcccctt
     6181 ttgagacttt tcttcagcta agggagaaat agagtaaaaa gttttgggat tatcttttcc
     6241 tagccttgaa ctaataagat catcgctatt aaggattaaa tgagtatggc taggaatatg
     6301 atcctttagt actctgaaaa caaattctcc gtgggcattt cttagatagg actccctaaa
     6361 tagattggta acaagaagat aatcgggggc catttcagga aaaataatcc gactggctct
     6421 ttcatctact tcaagtatac aaagttcgag tttaggacca ccaaaaaatg ttgaggcatc
     6481 taaaagagtt gttataacac cctcttttat attgctgcca tagctattat gagcaaagct
     6541 aaagcccagt ttcttatata aatcagcaat taaattggag cttgtggttt ttccattagt
     6601 acctgtaatg gcaatcaccg ttttaggttt ggccaaatgg cttaaaaaat caggacatag
     6661 cttaagggct agcgctcctg gtgtatgtgt agcattatgc cctgttagct ttagaatcag
     6721 ggcaattaaa cgtgctaggg ttatggctaa ataaaagcgc aacttcttca ttgcttcctc
     6781 tttccatagg tataatatca caccattata ccatatggtt gtgctagatt tagcttcctt
     6841 agtttgacac cgatttacta atcttcaaat ctatttgctt cgtcgctgca agtcttagca
     6901 gcttttttct tgtcaatttt aaatcttttt gatgtacttc tatgtggttc tatggtataa
     6961 ttataaaa
//
LOCUS       DASRNA010000101         4203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_551770,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000101 DASRNA010000000
VERSION     DASRNA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551770"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..2384)
                     /locus_tag="VFD08_02755"
     CDS             complement(264..2384)
                     /locus_tag="VFD08_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09968.1"
                     /translation="MGQRKWTSIILILLMLILLTACGGGGGAAKFEDGKLALEEAKLD
                     FGNNEVTAEAVQMELIKTKEEEAPEGLQSDLYEVTIDLDLEESVTFSLPYKEDEEVED
                     ENAQLVLGIGREYEYDDGRKDTVYSYLPGEHKDGQFTATFIPAEALEELKFNGAVGGG
                     KASGRKLSLGIFRSSMWFKEGGHFELSFPHTSANFRTFLECGEQLLHDLEDIYQEYLG
                     KGYNYNERSSWPMEVSVQKISALGYYSYGWKSANGKILLNLDLFKDGYQRDRVISLLA
                     HEFFHFVQGNYATTFNSCTWLDEATATYFEGEKLGNTPAIFQEHREKIFRGIIPENDS
                     AEEGYARQPVIKYLAKKNGEDFIRKAYESGKAGSSWEAAFKTSAGDLAMWAPEFYDAL
                     VRGEVGTYVPYTIHRELTESGSDIGQTLALTIPNKEELTVMVENDEIPSLGESTLEVD
                     GFGAQLLALTIEQDQIENLADGIDPIVTVDPYARVKVYDILSSNVQAFPQEADQVVIN
                     NFKEGLEQDHLYLVLVTGIHSSGKTPYTVRVDLQPYPTLDEVVGIYKDGALFISEVEI
                     SPELEAEAQEESTEDSGSGEVYGMDVGCDLDMINLLKGLEGQTMPRILDIKKTAEAAG
                     SLLLIEDLADPDDRPMAFSFSNGVLNFDTADGEVADITGEIVASYGKNKEIVLSGTLK
                     MTFSGDENTWILMNVTGSKPLATP"
     gene            complement(3052..3861)
                     /locus_tag="VFD08_02760"
     CDS             complement(3052..3861)
                     /locus_tag="VFD08_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK09969.1"
                     /translation="MKKELMVDTFDGVKLFMAKSLPEDRVAVAVVVHGLAEHSGRYKY
                     LVEKLNDANIGVYRFDHRGHGQSEGEESHYEDFNELTDDVNLVVELAKGENPDLPIFV
                     IGHSMGGFGVASFGSKYPNSVDGIVISGGLTRDHHGTISNVPKDLADDFRLANELTDG
                     VCSVEEVRIDYVNDPLNKKTFTVGLARSLARGVEWLKKSAEVFTDPVLILHGQLDSLV
                     SYKDSLDFFSEISSKDKQVKIYGNLFHEIFNEYAKDEVIGDVIGWIEKRIK"
BASE COUNT         1220 a          970 c          691 g         1322 t
ORIGIN      
        1 atcaaaaact attattggct atctttatgg gcctatttgt tttaatcctc tcttcaggaa
       61 taatctattt gctaacaaga tttttctaac aaaaataata agagaagtta agtaaaacta
      121 taagggacat actaatccaa tctatcatag tcaggacaaa acaatacgca ctcctcacag
      181 tcaaccaaac actgtgaaga gtgcgcaagc tttaataaat gaaatactct tctctaaaaa
      241 gatttagaca aattatcctg tgtttaagga gtagccaggg gcttgctccc agtcacattc
      301 attaaaatcc aagtattttc gtcaccacta aaggtcatct ttaaagttcc actaagcacg
      361 atctccttgt tttttccgta ggaggcaaca atttctcctg ttatatcagc gacctcccca
      421 tctgcggtat caaagtttag tacaccatta gaaaagctaa aggccatggg cctatcatct
      481 gggtcggcta aatcttcgat aagcaaaagg ctacctgctg cttctgcagt ttttttgata
      541 tcaaggatcc ttggcatcgt ctgaccttct aaacctttta gaagatttat catatctaaa
      601 tcacaaccca catccatccc atatacttct cctgagcctg agtcttcagt gctttcctcc
      661 tgagcctctg cttcgagctc aggagatatc tcaacttctg aaataaagag cgccccatcc
      721 ttatatattc ctacaacttc atccagggtt ggatagggtt gtagatctac cctaactgta
      781 tagggagttt ttccactgct atggattcct gttacaagaa ccagataaag gtggtcttgc
      841 tctaacccct ctttgaagtt attaataacc acctgatcag cttcttgagg gaaggcttgg
      901 acattactac ttaggatatc atagactttt actctcgcat atggatctac agtaactata
      961 ggatctatcc catcggcaag gttttctatt tgatcttgct ctatggttaa ggcaagaagc
     1021 tgagcaccaa aaccatccac ttctaaggta ctttctccca gggagggtat ttcgtcattt
     1081 tctaccatca ctgttagttc ttctttattt ggaatggtaa gagccagagt ttgaccaata
     1141 tcagatcctg attcggttaa ttccctgtga atcgtatagg gaacgtaggt tccaacctct
     1201 ccccttacca gggcgtcata aaattcaggt gcccacatag ccagatctcc agcggaagtc
     1261 ttaaaagcag cttcccagct actaccggcc ttgcctgatt cataggcttt gcgaataaag
     1321 tcttcgccat ttttcttggc tagatatttg ataacaggct ggcgggcata gccctcctca
     1381 gccgaatcat tttctggaat aataccccta aatatttttt cccgatgttc ctgaaaaatt
     1441 gccggagtgt tgcctagctt ttcaccttca aagtaggttg ctgttgcctc atctaaccag
     1501 gtacaactat taaaggtagt ggcataattt ccctggacaa aatggaaaaa ctcatgagcc
     1561 agtaggctaa ttaccctatc cctttgatag ccatctttaa aaaggtctaa attaagtagg
     1621 atcttcccat tggctgattt ccaaccatag gaataatagc ccaaggcact tatcttttga
     1681 acggagacct ccatgggcca actactacgt tcattatagt tatatccctt acccaggtac
     1741 tcctggtaaa tatcctcaag atcatgtagt agctgctctc cacactctaa aaaggtccta
     1801 aagttggcag aggtatgggg aaaactaagc tcaaagtgcc ccccttcttt aaaccacatt
     1861 gaagacctaa aaattcctag agaaagcttt cttccactgg cctttccccc gcctactgcc
     1921 ccattaaatt taagttcttc caaggcctcc gctgggataa atgtggcagt aaattgtcca
     1981 tccttatgct cacccggtag gtagctatag acagtgtctt ttcttccatc atcgtattca
     2041 tattctcttc ctatacctag aacaagttga gcgttttcat cctctacttc ctcatcctct
     2101 ttatagggaa gggaaaaagt aacgctttct tctaggtcaa ggtcgatggt cacttcatat
     2161 aggtctgatt gtagcccttc tggcgcttcc tcttcttttg tttttataag ctccatttgg
     2221 acagcttctg ctgtaacttc attatttcca aaatcaagtt tggcttcctc tagtgccagc
     2281 tttccatctt caaattttgc ggccccacct ccgccaccac aggcagttag taggatcagc
     2341 atcagaagaa tcagtatgat agaagtccat tttctttgac ccatttatct cccctttcgt
     2401 cccatcaatt aaagaaaaca caaataaaaa atcagtttta tttattaata tattatcatg
     2461 ttttaacaat taaaacaaca gcgtgtaaca tttgatgttg gggatttaca tatagctaac
     2521 ttttcaaagt aaacgcaaca cttgccttag gaaacactcc cccctccttt accttgagaa
     2581 actgtgtaaa ctaaaaacaa gcttctttat agccaagtat tcttagcctc ctagggccaa
     2641 taagccagct ttttttcctt taaacctttt ttaaaaggat tccttggctg tctttttgtt
     2701 tttttccttt ataatttggg agttttccta aaaaagaagg tggaaaaaga aaaaggaccc
     2761 taccaaaaaa tgataaagtc cttagtcaag atagttagct tgtaccatgc cgggccacca
     2821 tactggcaag aacacaggga ctggtccctc tagcttcttt ttttcttctt gtgagcttgt
     2881 tagagggtct atattagcct ggcaaacact aagaaaaaag cgataagcta taggtcttta
     2941 tttagaagaa agtagtcata aagagagggg cttaactagc ccccccctta cattctatta
     3001 ctatagattt tttcctttat ttatccagcc agcaataata gaaaagccct attatttaat
     3061 tctcttttca atccacccta taacatcacc gattacttca tcttttgcgt attcattaaa
     3121 tatttcatga aaaaggttgc catatatttt cacttgttta tccttagaag aaatttcact
     3181 aaagaagtct agagagtctt tataggatac tagggagtca agttgaccat gtaagatcag
     3241 cacaggatca gtaaatactt ctgcactttt cttcaaccac tccacccctc ttgctaggga
     3301 acgagccagg ccaacagtaa aggttttttt gtttagggga tcatttacat aatctatacg
     3361 gacttcttcc acagaacaaa ccccatctgt tagttcattt gctaggcgaa aatcatctgc
     3421 tagatcttta ggaacattag aaatagtgcc atggtggtct ctagttaatc cacctgatat
     3481 aacgattcca tctactgaat tgggatattt gcttccaaaa gaagccaccc caaaaccgcc
     3541 catactatga ccaatgacaa atatgggaag gtcgggattc tcacccttgg ctaactccac
     3601 cacaagattt acatcatcag ttaattcatt gaagtcttca tagtgacttt cttctccttc
     3661 tgattgacca tggcccctgt gatcaaaacg atatacccct atattggcat cgtttaattt
     3721 ttctaccaga tacttatacc tgccagagtg ctctgccaga ccatgaacca ccacagcaac
     3781 cgctacccta tcctctggta gggattttgc cataaatagc tttacgccat caaaagtatc
     3841 taccatcagt tcttttttca taattggcct ccttgctttc ttctctaatg aaatgttttt
     3901 cctatctaga agtcttttct actacttata gataccccct aaatgaggat tttaagattt
     3961 tattgtaata ttcttttact tgattaggca gtctaagatc tttaggccca ttataagagg
     4021 aactatcctc ttagattatc taccacttct ttttcatcaa acacttatta taaaaataaa
     4081 cagaaataaa tatgcttgat agagaaaatg caagaatact aactaggatc cctataaagt
     4141 tcatcccctt aagaaggctc cctagaccaa gagccatatc atctaaactg accaggtaaa
     4201 agg
//
LOCUS       DASRNA010000102         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_779404,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000102 DASRNA010000000
VERSION     DASRNA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779404"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..960)
                     /gene="cas1e"
                     /locus_tag="VFD08_02765"
     CDS             complement(25..960)
                     /gene="cas1e"
                     /locus_tag="VFD08_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HZK09970.1"
                     /translation="MTKIPGTKKPRLQELPRVSDRITFLYLEHAKINRQDGAITVTDS
                     RGIIRVPAVLVGVLLLGPGTDLTHRAMELIGDTGTSVVWVGEWGVRQYAHGRALSHNT
                     KLLINQAKLVSNVRSRVTIARKMYQIRFENEDVATLTMQQLRGREGARIRSIYRKESK
                     KSGVPWAGREYDPDDFAGGTPINQALSAAHAALYGLVYSIIVALGLSPGLGFIHTGHD
                     LSFVYDIADLYKADVTIPIAFKIAAKSDVTDIGRETRLAVRDAFVDGKIMKRIINDLH
                     ILFEEEGKNVVADLINLWDDKKGLVDHGVSYDEFS"
     gene            complement(965..1603)
                     /gene="cas6e"
                     /locus_tag="VFD08_02770"
     CDS             complement(965..1603)
                     /gene="cas6e"
                     /locus_tag="VFD08_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002268883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HZK09971.1"
                     /translation="MFYMSRVEIDTGNRRKISQLTHLGAYHNWVEKCFPEEIDRGKRS
                     RKLWRVDKLKGKSYLLVVSQAKPEIKGLEEYGVVGTGQCRDYQLFLESLEDGQRMSFR
                     LMANPVRAVKQDGQRGKIYPHITDKYQLEYLEKRAERLGFFLMENDYQLVQRDFPILR
                     KKGGKSIKLARAVYEGGLIIEDVEIFRKTLTQGVGREKAYGFGMMTVIPRGR"
     gene            complement(1603..2325)
                     /gene="cas5e"
                     /locus_tag="VFD08_02775"
     CDS             complement(1603..2325)
                     /gene="cas5e"
                     /locus_tag="VFD08_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HZK09972.1"
                     /translation="MMKTIILKFSGPLQSWGIGSNFETRDTERYPTKSAVIGLVAGAL
                     GYRRDEKEKISRLNELSFGVRVDQKGSLLKDYHIARSERQTYVTNRYYLQDAVFLAMI
                     SHEDSSWVDTVAKALLNPVFPPFMGRRSAPVDGQFFQGLVEGEVLSIIKSYQWEASDW
                     YQKKLTGKNQSGRIVLDAYVDANLLDDSSQPMFKRDRFESFDPMNRNHGFRPVKSLQV
                     EIQTKEVKKETEHDAFEGIGGL"
     gene            complement(2335..3393)
                     /gene="cas7e"
                     /locus_tag="VFD08_02780"
     CDS             complement(2335..3393)
                     /gene="cas7e"
                     /locus_tag="VFD08_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HZK09973.1"
                     /translation="MNNNIYMDLHVIQTVPPSNINRDDTGSPKTAKYGGVTRARVSSQ
                     SWKKAMRDYFKKYSDKDTLGIRTLKIVHYLAEAIMEEEQSIEEEDALKLAEEIFKKVK
                     ISTKNQEARALFFLGGEQRKQLASYGVEHADNLQVGEIKNILNANQSIDVALFGRMVA
                     DAPDLNVDASSQVAHSISTHEIETEFDFFTAIDDLTPDDQSGAGMLGTVEYNSSTLYR
                     YANVAIHELNQQLDSNKEVVIEALKLFIKAFALSIPSGKINTFANQTLPNALVVTLRQ
                     DRPVNLVNAFESPVKAKNGYVEGSISKFKEEYEKTNRFIEAPLATFSTGDIEGLGQGI
                     GSLNELLAAFEESANKLL"
     gene            complement(3386..>3927)
                     /gene="casB"
                     /locus_tag="VFD08_02785"
     CDS             complement(3386..>3927)
                     /gene="casB"
                     /locus_tag="VFD08_02785"
                     /inference="COORDINATES: protein motif:HMM:NF021034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HZK09974.1"
                     /translation="IIYKLGNRLELSETKATLSHLRNSIGRDLSETVAVWPLMFEEVS
                     QEYLSRSGKPTYQENAILSSLQLYALHQQGKNKAVHDSSGDSVGKALSNVRDRENVAL
                     DRRFNAMVVSGRFKELTTHLRHLISILKQKTETKIDYAKLAEDLYWYQVSEESANRMR
                     MRWGQDYYFTQGKKEEKDE"
BASE COUNT         1073 a          928 c          578 g         1348 t
ORIGIN      
        1 accatagact taagtcagcc tcctctatga aaattcatca tatgaaactc catggtccac
       61 aagtcctttt ttatcgtccc aaagatttat taaatcagca actacatttt ttccctcttc
      121 ttcaaaaagg atatgtaaat cgttgattat tcttttcata attttcccat ctacaaaagc
      181 atctcgaacc gccaatcgtg tttctctacc aatatctgta acatctgatt ttgctgcaat
      241 tttaaaagca atgggtattg tcacatcagc cttatataaa tccgctatat cataaacaaa
      301 agataggtca tgccctgtat gaatgaagcc taatcctggt gacaatccaa gtgccacaat
      361 aatactatag actaggccat ataatgctgc atgggctgct gatagggctt gattaattgg
      421 agtccctccc gcaaaatcat cagggtcata ttctctacct gcccaaggaa ctccactctt
      481 ttttgattct tttctataaa tacttctaat tctggcacct tctctgcccc taagttgttg
      541 catagttaag gttgcaacat cttcgttttc aaatcttatt tgatacattt ttcttgctat
      601 ggttactcta gacctaacat ttgatactaa ctttgcctga tttatcagta gcttagtatt
      661 atgtgacaag gctctaccat gggcatattg cctaaccccc cattcaccaa cccatacaac
      721 actggttcct gtatctccta tcagctccat tgctctgtga gtaagatctg ttccaggccc
      781 aagcagtaat actcctacaa gtacagctgg aacacgtata atacctctac tatctgtaac
      841 tgtaatagcc ccatcttgtc tgttgatttt tgcatgctcc agatataaaa aggtgatcct
      901 atcacttacc cgtggaagtt cttgtaatct tggttttttt gttccaggta ttttagtcat
      961 atttctacct tcctctcggt attacagtca tcataccaaa accatatgct ttttcccttc
     1021 caacgccttg agttaaggtc tttctgaaaa tctcaacatc ctcaataatt aaaccacctt
     1081 cataaactgc ccttgctaac ttaatgcttt tcccaccctt tttcctaagt attggaaagt
     1141 ctctttgcac aagctggtaa tcattttcca tcaagaaaaa tccaagcctc tcagcccgct
     1201 tctccaagta ttctagctga tatttatctg taatatgggg ataaattttt cccctttgac
     1261 catcctgctt aacagctctt acaggattag ccatcaacct aaaggacatt ctttggccat
     1321 cttctagact ttctaggaag agttgataat ctctgcattg gcctgtacca acaaccccgt
     1381 actcttccaa tccttttatt tctggttttg cttgactaac aaccaaaaga tagcttttcc
     1441 ctttcagttt atcaactctc cataacttac gacttctttt ccctctatct atttcttcag
     1501 gaaagcattt ttcaacccaa ttgtgataag ctcccaggtg ggttaattgt gaaattttcc
     1561 tcctgttccc agtatcaatt tcaactcgtg acatataaaa catcataggc ctcctatccc
     1621 ttcaaaggca tcgtgttctg tttctttttt tacttctttg gtttggattt ccacctgcaa
     1681 agactttact ggcctaaatc catggtttct attcatagga tcaaaggatt caaacctatc
     1741 ccgcttaaac ataggctgtg aagaatcatc tagtaaattt gcatctacat aagcgtctaa
     1801 gacaatcctg ccagattgat ttttaccagt caattttttc tgataccagt cactggcctc
     1861 ccactgatag gattttataa ttgataaaac ttctccttct acaagccctt gaaaaaattg
     1921 accatctact ggagcactcc tccgccccat aaaaggagga aagactgggt taagaagtgc
     1981 ttttgccaca gtatcaaccc agcttgaatc ttcatgagat atcattgcaa gaaaaactgc
     2041 atcctgtaaa taatatcgat tggtaacata agtctgcctt tcagacctgg caatatgata
     2101 gtcctttagc aaggaacctt tttgatctac ccggactcca aatgatagtt catttagcct
     2161 actaatcttt tccttttcat cccgacggta tcccaacgca ccagctacta agccgattac
     2221 tgcactttta gtaggatacc tttccgtatc ccttgtttca aaattagatc caatccccca
     2281 tgattgcagt ggacctgaaa atttcaaaat aattgttttc atcataatcc tcacctataa
     2341 aagcttattt gctgactctt cgaatgctgc caacaattca tttaagctgc ctatgccttg
     2401 tcctagtccc tctatatcac ctgttgaaaa tgttgcaaga ggagcctcaa taaatctgtt
     2461 agtcttttca tattcctctt taaactttga gattgatcct tctacatatc catttttagc
     2521 tttaactgga ctctcaaatg catttactaa atttacagga cggtcttggc gaagagttac
     2581 caccaaggca ttgggaagcg tctgatttgc aaaagtgtta atctttccac ttggtataga
     2641 taaggcaaat gctttaatga atagctttaa tgcttcaatt accacctctt tattactgtc
     2701 tagctgttga ttcagttcat gtattgctac atttgcatat ctatacaagg ttgatgagtt
     2761 atattccact gtccccaaca tacctgctcc tgactgatca tcaggagtta agtcatcaat
     2821 agcagtaaaa aaatcgaatt ctgtttctat ttcatgagta gatatagagt gggcaacctg
     2881 tgagcttgca tccacattta agtctggggc atctgcaacc atacgcccaa acaatgccac
     2941 atcaatgctt tggtttgcat ttaaaatatt ttttatctct cctacttgca agttatcagc
     3001 atgctccact ccgtaagaag caagttgctt tcgctgctct ccacctagaa agaaaagtgc
     3061 tctagcctct tgattctttg tagatatttt cacctttttg aaaatttctt ccgctagttt
     3121 aagtgcatcc tcttcctcta tggactgttc ttcttccata attgcctctg caagataatg
     3181 aacaatttta agggtcctaa ttcctaaggt atctttatca gaatattttt taaaataatc
     3241 tctcatggct tttttccaac tttgagatga tacccgagcc cttgtaacac ctccatattt
     3301 agctgtctta ggactaccag tatcatccct gttaatgttg cttggtggaa ctgtctgaat
     3361 cacgtgtaag tccatataaa tattattatt catctttttc ctcctttttt ccctgtgtaa
     3421 aataataatc ttgcccccaa cgcatcctca tacgattagc agattcctct gatacttgat
     3481 accagtataa atcctctgct agtttggcat aatcaatttt tgtttctgtt ttttgcttaa
     3541 gtatgctgat tagatggcgt agatgagtag tcaattcttt aaatctgcca gaaacgacca
     3601 tcgcattaaa ccttctatcc aaggccacat tttccctatc tcttacattg cttaatgctt
     3661 ttcctacact atctcccgat gaatcatgca ccgctttatt cttaccctgc tggtgcagcg
     3721 cataaagctg caaggatgat aaaattgcat tctcttgata ggtaggtttt ccacttctac
     3781 tcaaatattc ttgtgacact tcctcaaaca tcaaaggcca aactgcaact gtctctgata
     3841 aatctcgccc tatagaattt ctcaagtgag ataaggttgc cttggtttca gacaattcaa
     3901 gtctatttcc tagcttatat ataatcc
//
LOCUS       DASRNA010000103         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_402298,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000103 DASRNA010000000
VERSION     DASRNA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402298"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /gene="hisS"
                     /locus_tag="VFD08_02790"
     CDS             <1..996
                     /gene="hisS"
                     /locus_tag="VFD08_02790"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZK09975.1"
                     /translation="APTMRSFIENKIYADAQPTKLFYMGSNFRNERPQAGRFREFTQF
                     GIEYIGSEDAYCDAEVIGFAMDMMQRLEITDNELRINSVGCPSCRHIYHEHLRSFLAD
                     KVEHLCHDCQERYDINPLRILDCKVEADQKRIKGYPVLLDHLCEECAGHFKKLEEYLT
                     AMELDYTVDTSIVRGLDYYTKTAFEITSSSLGSQSTICGGGRYDGLIEQLGGPPTPGV
                     GFGMGMDRLLMAVKEEEEEETRKGILLLALGEKQKPPLINLGVKLRKAGHYTELEVMN
                     RSLRASMKHANKRLYKIVLVYGESEKDKGVILYRDMDKGEQIEVEFDKIVEVLSE"
     gene            989..2755
                     /gene="aspS"
                     /locus_tag="VFD08_02795"
     CDS             989..2755
                     /gene="aspS"
                     /locus_tag="VFD08_02795"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZK09976.1"
                     /translation="MSSLLRRSCYSTQIGSEMIGQELIFNGWVAGKRNLGSLVFVDLR
                     DREGLLQLVFDEEVDQELYLRASKLGREYVIGVKGILRKRDSVNPELKTGDVELKVTE
                     LEVFSRSEVPPIHVREDDGAKEELRLKYRYLDLRKKKNMDRLRLRARVNQVLREYLIK
                     EDFTEFETPILNKTTPEGARDYLVPSRVNPGKFYALPQSPQIFKQLLMISGADKYYQI
                     TRCFRDEDLRADRQPEFTQLDIEMSFVDQERVMEVNEGLIHHLFKEIAGVDIPLPLRR
                     MSYDEAMQRYGSDKPDTRYGMELLALEDVFRNSDFRVFSSAIKEGKSVRGIFVPGEGL
                     FSSKEIKKVEKSAKTYGAAGLATVILEEEIKGSIARFLGDKEIASLRALAGGEKGTFF
                     IVSDEKKVVLTVLGNLRRSIAREKELFNPKEFDLIWIVDFPAFHFDEEEQRYVAEHHP
                     FTMPKEEDLVYLDSDPLRVKANCYDLVINGFETASGSIRIHDSHLQSKIFDVLGFTRQ
                     AARHRFGFFIDALKYGTPPHGGIAYGLDRLTMIFTETENIRDVIAFPKSLQATCLMSE
                     APSYVEKDQLEELHIELISHED"
     gene            2730..3176
                     /locus_tag="VFD08_02800"
     CDS             2730..3176
                     /locus_tag="VFD08_02800"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HZK09977.1"
                     /translation="MNLFPMKIKAKVLDYRNGKAHLETIPEGECVGCKGCSQSNVKEL
                     TVESSPIEVGSNVWISMETSNVTRAALLAYGLPLALFMAGLFLGIALFKGSRYDSISE
                     LIGFAFGLVVFFSTYFIIRRGEIRRQADQQYQAKIISYTEEEIWNQ"
     gene            3164..4387
                     /gene="glyA"
                     /locus_tag="VFD08_02805"
     CDS             3164..4387
                     /gene="glyA"
                     /locus_tag="VFD08_02805"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZK09978.1"
                     /translation="MESIKKFDPKLYTMMQMEKNRQEEGLELIASENFVSQSILEALG
                     SVLTNKYAEGYPGKRYYGGCEFVDLAENLARDRMCELFGADHANVQPHAGSQANMAAY
                     FALIKPGDKVLGMALDNGGHLTHGSPVNFSGQLYDISSYGVDDNGYIDYGEVEKVALE
                     VKPKLIIAGGSAYAREIDFKRFKEIADMVGAYLLVDMAHIAGLIAAGEHMNPVPYADV
                     VTTTTHKTLRGPRGGAILCKAEHARAIDRSVFPGMQGGPLEHAIAAKAVCFKEAMEPS
                     FKDYIIQVKKNAHVLAQTLMELGYDVVSGGTDTHLLLVDLRSKNLTGKEAEALLGGVD
                     ITVNKNTVPKETESPFVTSGIRIGSAALTTRGMKEEEMKKTAKLIDEAMMKSRDPEAI
                     LADVKALAAEFPLYK"
BASE COUNT         1385 a          765 c         1019 g         1254 t
ORIGIN      
        1 gctccaacca tgcgcagttt tattgaaaat aaaatttatg cggatgctca gcccacaaaa
       61 cttttttaca tgggttcaaa ctttagaaat gaaagacccc aggcaggcag atttcgcgag
      121 tttacacagt ttggcataga gtatatagga agtgaagatg cctattgcga tgctgaggtt
      181 attggctttg caatggatat gatgcaaaga cttgaaatta ctgataatga gctaagaatt
      241 aactctgtgg gctgtcccag ttgtcgacat atttatcatg aacacctaag aagtttttta
      301 gctgataagg tggagcactt gtgtcatgat tgtcaagagc gctatgatat aaaccccctg
      361 cgtattcttg attgcaaggt ggaagctgac caaaaaagaa ttaagggtta tcctgtcctt
      421 ttagatcatc tttgtgagga gtgcgcaggg cactttaaaa aactcgaaga atatcttacg
      481 gctatggagc ttgattatac agtggataca agtattgtcc gggggcttga ctactatacc
      541 aaaacagcct ttgagattac ctcttcctct ttaggtagcc aatctaccat atgtggtgga
      601 ggacgttatg atggtcttat tgagcaactt ggtggacctc caacacctgg tgttggtttt
      661 ggcatgggga tggatagact tcttatggca gtaaaagaag aggaagaaga agaaacaaga
      721 aagggaatcc tgctactagc cctaggtgaa aagcaaaaac caccccttat taatctagga
      781 gtaaaacttc gtaaggccgg tcattatact gagcttgaag ttatgaatag aagtcttcga
      841 gcatcaatga aacatgcaaa taagcgcctc tataaaatag tcctagtcta tggtgaaagt
      901 gaaaaagaca aaggggttat cctctaccga gacatggata aaggggaaca aatagaagtg
      961 gaatttgata aaatcgtgga ggttttaagt gagtagttta ttaagacgaa gctgttatag
     1021 tacgcaaata ggttctgaga tgattggcca agagctaatc tttaatggct gggtagccgg
     1081 gaaaagaaat ttagggtccc tagtttttgt tgatttaaga gatagagagg gacttcttca
     1141 gcttgttttt gatgaagaag tagatcaaga gctctatctt agggcatcaa aactgggccg
     1201 tgagtatgtt attggagtaa agggcatact aaggaaaaga gattcagtaa atcctgaact
     1261 taaaacagga gatgttgaat taaaagtaac tgagctagaa gtttttagca gatcagaagt
     1321 tcctccgatt catgttagag aggacgatgg agcaaaagaa gagctaagac ttaagtatcg
     1381 ttatctagat cttcgcaaga aaaagaacat ggaccgtcta cgtttaaggg caagggtcaa
     1441 tcaagttctt agggaatacc ttatcaaaga ggacttcacg gaatttgaaa ctcccatctt
     1501 aaataaaaca acaccagaag gagctagaga ctatctagta cccagtaggg tcaatccagg
     1561 caagttttac gccctacctc aatcgcctca aattttcaaa caacttctca tgatttctgg
     1621 agcagataag tattatcaaa ttactcgctg ttttcgtgat gaggatttac gcgcagaccg
     1681 tcagccagaa tttactcaac ttgatataga gatgagtttt gttgatcaag aaagagtaat
     1741 ggaagtcaat gaagggttaa ttcatcattt gtttaaggaa attgcaggag tagatattcc
     1801 tcttccacta aggcgaatga gttatgatga ggccatgcaa agatatggca gtgataaacc
     1861 tgatactcgc tatggtatgg aactattggc cctagaagac gtctttagaa attctgattt
     1921 ccgcgtcttt tctagtgcta ttaaagaagg aaaaagcgtg cgaggaatct ttgtaccagg
     1981 agaaggccta tttagctcaa aggaaattaa aaaggtggaa aaatccgcca agacctacgg
     2041 agcagcggga cttgctacag ttatcctaga agaagaaatt aaaggcagca ttgcaagatt
     2101 tttaggtgat aaggaaattg catccttaag agccctggct gggggagaaa aaggaacctt
     2161 ctttattgtc tctgatgaga aaaaagttgt tcttactgtt cttggcaatc taagaagaag
     2221 tattgcccga gaaaaagagc tttttaaccc caaggaattt gacttgattt ggattgttga
     2281 ttttcctgct tttcattttg atgaagaaga gcagcgctat gtggcggaac atcacccctt
     2341 taccatgcct aaagaagaag atctggtcta tctagatagt gatcctctac gagttaaggc
     2401 aaactgctat gatcttgtga taaatgggtt tgaaacggcc tcaggaagta ttcgtattca
     2461 tgatagtcat ttacagtcaa aaatctttga tgtattagga tttacaaggc aagcggcccg
     2521 tcatcgtttt ggctttttta ttgacgccct taagtatggc actcctcccc atggtggtat
     2581 tgcctatggc ctcgaccgct taaccatgat ttttactgaa acagaaaata ttagagatgt
     2641 tattgccttt cccaagagcc ttcaggcgac ttgtcttatg agtgaagctc ctagctatgt
     2701 tgaaaaagac cagctagaag aacttcatat tgaacttatt tcccatgaag attaaagcaa
     2761 aagtccttga ttacagaaat ggcaaggccc atctagaaac aattcctgag ggagaatgtg
     2821 tgggatgcaa gggctgctcc caatcaaatg tcaaagaact aacagtagaa tcatcaccca
     2881 tagaagtagg cagtaatgta tggataagta tggaaacaag caatgtcaca cgggcggcac
     2941 ttctggccta tggtttacct ctagcacttt ttatggctgg actattttta gggattgcac
     3001 tttttaaagg aagccgttat gatagtataa gtgaactgat tggttttgca ttcggcttgg
     3061 ttgttttctt ttctacttat tttataataa gaagagggga gataagaaga caggcagatc
     3121 aacagtatca agcaaaaata attagctata ctgaggagga aatatggaat caataaagaa
     3181 gtttgatcca aagctttata caatgatgca aatggaaaaa aaccgtcaag aagaaggtct
     3241 tgagttaatt gcttcagaaa actttgtgtc ccagtcaatt ttagaggcac taggtagtgt
     3301 attgacaaat aaatatgctg aaggatatcc tggcaaacgc tattatggtg gatgtgagtt
     3361 tgttgacctg gcagaaaact tagcaagaga tcgtatgtgt gagttatttg gtgccgatca
     3421 tgcgaatgtc cagcctcatg ctggtagtca agccaatatg gctgcctact ttgccctgat
     3481 taaacctggc gataaagtac ttggtatggc ccttgataat ggtggtcatc ttacccatgg
     3541 ttccccagtg aacttttctg ggcaactata cgatatctcc tcttatggtg ttgatgataa
     3601 tggttatatc gattatggtg aagtagaaaa agtagctctg gaagtcaaac ctaaattgat
     3661 aattgctggt ggcagcgcct atgcaagaga aatagacttt aagcgcttta aagagattgc
     3721 tgatatggtt ggtgcctatc tacttgtgga tatggctcat atcgccggtc ttattgctgc
     3781 aggggagcat atgaatcctg ttccctatgc tgatgttgta accactacga ctcacaaaac
     3841 ccttcgtgga cctagagggg gagcaatttt atgtaaagct gagcacgcta gggcaatcga
     3901 tagatcagtt ttccctggaa tgcaaggggg acctttagag cacgctattg ctgctaaggc
     3961 agtttgcttt aaagaagcca tggaaccatc ttttaaagac tatattattc aggtaaagaa
     4021 aaatgcccat gttttagccc agacccttat ggaacttggc tatgatgtgg taagtggtgg
     4081 aacagacacc catcttcttc ttgtagacct tcgctcaaaa aacctaacgg gtaaagaagc
     4141 ggaagctctt ttaggtggag ttgatattac agtcaataaa aacactgttc ccaaagaaac
     4201 agaatctcct tttgttacca gtggtattcg tattggatct gctgccttaa caacccgggg
     4261 gatgaaggaa gaagaaatga aaaaaacagc taagcttatt gatgaagcta tgatgaaaag
     4321 tcgtgatcca gaggccattt tagccgatgt taaagcttta gccgcagaat ttcctcttta
     4381 taaataacct ataacactat taagttaaga tgcacttttg ggt
//
LOCUS       DASRNA010000104         6382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1075012,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000104 DASRNA010000000
VERSION     DASRNA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075012"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFD08_02810"
     CDS             <1..1015
                     /locus_tag="VFD08_02810"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09979.1"
                     /translation="NGGLWVEPPVFRSDLILEADLIEEVARIYGYENIPSSLPSIPEP
                     AALTPRQKFEWDLRDLLWGLGFEEMLNYSFVSPKSAASIYRDTEHPLRLINPLGEEFS
                     QMRHSLLISALDVLQRNENIKNQAVNFFEIGNVFEVKEDIEQRQMFSLARYGLGDFFN
                     LKGVLEELFASLGIEALYKRSKEAVYHSGRSADVYVGEKLIGSFGELSPFVALERGIK
                     ESVYLGEFSLDCLFELKDHKIIHKAPSRYPAVVRDLALLVDKDLTQEEIYDKILSQGG
                     KLLKKVELFDIYYGQELGEDKKSMAYHCKFQSSERTLKDKEVEDAMARILTGLEEIGA
                     QRR"
     gene            1024..1401
                     /locus_tag="VFD08_02815"
     CDS             1024..1401
                     /locus_tag="VFD08_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09980.1"
                     /translation="MSKRVTMDIDGANVVMVTSAPKERVDQVIKQVNEMITLVKSKDR
                     SLSSNMAYRYAMVYLSDQIVELKEVLDSDGDGAFSDNKNIDLKREIAKLKEEVLQWEG
                     RTAQLQELLLEKNQQIQELKSLR"
     gene            1418..3751
                     /locus_tag="VFD08_02820"
     CDS             1418..3751
                     /locus_tag="VFD08_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HZK09981.1"
                     /translation="MNKNAVRALEFDKIKQALMEHMPSPGSCAQIEKQGFAKSPQELQ
                     DRQEILREALGYLLSYSPLPFGPLKDMRDELGYAKRGGVLEPLSLLSIGDSLRSISRM
                     KLFLEKHSERREDFRILYNYGALLENFSHLEKLIEITVDYDGSIKSTASRTLAGIRRN
                     IQSKEEEIRRKIDSFLNDRNMEGYLQESLVTMRNDRYVLPIKSEHKRRVEGLVHDRSG
                     SGSTLYIEPIALVNLNNDLRELRSDEKEEIHKILKEISLRLGQEESIIPAYMATEELY
                     RVFGMANYALEHDSHLAKTGEKILLKKARHPLLDQKTVVPMDLDFGEEARILIITGPN
                     TGGKTVALKTLGLFSLMHQFGMAVPCDDGSVLPFFDDIFVDVGDEQSIEQSLSTFSSH
                     MVNLISILQEAGPNSLVLLDELGAGTDPQEGAALARSLLVEFEEKGSMVFATSHYSEI
                     KNFAFKRPGYMNSSVEFDAKTLSPTFRLLMGVPGSSNALYISQRLGLPERIIERARSS
                     MDDQGLAMEEILAEIEEKRLHQEELELKARRSLEEAKKLEEEFEEKLRHQQEQRDREL
                     LKAKKEAAKLLKQTEEEARDIMKNLRSMKGKVDFSSMEKQSQRLGQLKENLQDQTEAR
                     PIKAPAKLKVGEKIFIPHLNTIGEVLDKPNKKGSFNAQVGIMRLKLNISQVEKSKVSQ
                     KQIVKKQVQQFVASGVKTSLDLRGKNSEDARVELGIFIDQALLSNPPELEIIHGKGTG
                     VLRDFVKDYLKKSPFVKSFRQGGHHEGGSGVTLVKLH"
     gene            complement(3748..4497)
                     /locus_tag="VFD08_02825"
     CDS             complement(3748..4497)
                     /locus_tag="VFD08_02825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZK09982.1"
                     /translation="MNKKFTATKLYISINILIFLMIYTIDPTVSVVTLIDFGAQNNFS
                     LADGKLWHLIMPMFLHASWQHLGLNCLSIYIFGRFVEGYYGPKRFVLLSLAMGIIANL
                     GSFLSSPKVSVGASGVVYGYFAFHLYLYFLNKTRYKEYFGRDIFILLAINIVYSIFGT
                     NIDLAGHVFGLFGGFALYLLTDKQKVKKRTKQLISVLLLFLLLFSGGKMYSYRGSEDY
                     YLSKIYYYNQKNELILRDELIDEYLQKFPVP"
     gene            4737..>6382
                     /gene="argS"
                     /locus_tag="VFD08_02830"
     CDS             4737..>6382
                     /gene="argS"
                     /locus_tag="VFD08_02830"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZK09983.1"
                     /translation="MKKLKNELALILEPFLKEYLSLEEIEETLELPKFAEQGDLAFPC
                     FKLAKSMRKSPHIIAQELSQEISSDKFEVKNQGPYINFYINRQELAETILTEILSKKE
                     DYGSSEEGQNRPVIIEYSSANISKELHFGHIRGIMIGSSLYKITKTLGYKAIAINHLG
                     DYGINFGKIITAYKHWGDKEHIEKIGVRGLLELYLYFNKASAEDPSYMEEARRWFYKL
                     EEDKDPEALELWKWFKDVSLKEYNRVYKLIGCTFDSYAGEAFYSDMMPDIRQELLEKD
                     LIIEDDGAEIIDLSDENLPNVIVTTSQGSSLYITRDIAAAIYRKQEYDFYRNCYVVGS
                     EQRLHFTQLKAILKKMGYDWWDQCEHIDHGLVMMETGKISSRDGDNIFLEEVIQTAIQ
                     KTRDSIEERNPELEDKDEIARQVGLGALAYRELSNSRVKDYTFNWDETISFEGETGPY
                     LQYTNVRCQSLLTKGAYDESLGFDGSHLTEDSSKELLWELGLLPSILTSAFEKMEPAL
                     LSRYLMGVAKLFNKFYAQVSVIVDNKEEKNSKLALVKAVST"
BASE COUNT         2138 a         1117 c         1434 g         1693 t
ORIGIN      
        1 aaatggtggt ttatgggtag agccgcccgt ctttaggagt gatttgattc ttgaagcgga
       61 tttgattgag gaagtggcta gaatctatgg ctatgaaaat attccatcta gcctaccatc
      121 gattcctgag cctgcagctc ttacgcctag gcaaaaattt gaatgggatc ttagggatct
      181 tctctggggt cttggctttg aagaaatgct aaactactcc tttgtatctc ctaaaagtgc
      241 agcaagtatt tatagagata ccgagcatcc tctaaggctg attaatcctt taggcgaaga
      301 gttctcacag atgcgccata gccttctaat aagtgctttg gatgtcttgc aaagaaatga
      361 aaatataaaa aatcaagcag taaacttttt tgaaataggc aatgtctttg aagtaaaaga
      421 agatatagag caaagacaga tgtttagcct ggctagatat ggtctgggtg atttctttaa
      481 ccttaagggg gttctagaag aactatttgc ctccctggga atagaagctt tgtacaaaag
      541 aagcaaggaa gcggtttatc atagtggccg atctgctgat gtctatgtgg gagaaaaact
      601 tataggaagc tttggtgagc tttcaccctt tgttgctctt gagcggggga ttaaagaatc
      661 ggtttacttg ggtgagtttt ctctggattg tttatttgaa ctaaaagacc ataaaatcat
      721 ccataaagcc cccagtcgtt atccagctgt tgttcgtgac ctggctcttc ttgttgacaa
      781 agacctgacg caagaagaga tttatgataa aatactctca caggggggaa aactccttaa
      841 aaaagttgaa ctctttgaca tttactatgg acaagagctg ggagaggata aaaagagtat
      901 ggcctatcac tgtaagttcc aaagcagtga gcgtaccctt aaagataaag aggtggaaga
      961 tgccatggcc cgtattctta cgggcttaga agaaatcggt gcacagcgcc gctaggagga
     1021 aaaatgagta aacgtgtaac aatggacatt gatggtgcca atgttgtaat ggtgaccagt
     1081 gctccaaaag agcgtgtaga tcaggtgatt aaacaagtaa atgagatgat cacccttgtt
     1141 aaaagcaaag atcgttccct ctcttcaaat atggcctatc gctatgctat ggtctatcta
     1201 tctgatcaga ttgttgaact aaaagaagtt ctagatagtg atggagacgg tgcctttagc
     1261 gataataaga atattgatct taaaagagag attgccaagc taaaagaaga agtgctccag
     1321 tgggaaggaa gaacagccca actacaggaa cttctattag aaaagaacca acaaatacag
     1381 gagctaaaat ccctacgcta gttcctaggg aggtataatg aataaaaatg cagtacgggc
     1441 tctggagttt gataaaataa agcaagctct tatggagcat atgccatctc caggaagctg
     1501 tgcacaaata gaaaaacagg gctttgcaaa aagcccccaa gaactgcaag atcggcagga
     1561 aattttaagg gaggctttgg gctatttatt gtcctatagc ccccttcctt ttggtcccct
     1621 aaaagatatg cgtgatgaac tgggctatgc taaaagaggg ggggtacttg aacccctaag
     1681 tcttctttct atcggagata gcctgcgcag tataagcagg atgaaattat ttttagaaaa
     1741 gcatagtgag cgcagagaag actttcgcat tctttacaat tatggggccc ttcttgagaa
     1801 tttttctcac ctagaaaaac tgatagaaat tacagtggat tacgatggaa gcattaaatc
     1861 cactgcatca aggactctgg ctggtattcg ccggaatata caatccaaag aagaagaaat
     1921 tagacgtaag attgacagct ttctaaatga tcgaaatatg gaaggctatt tgcaggagtc
     1981 cctggttacc atgagaaatg acagatatgt attacccatt aaatctgagc acaagcgccg
     2041 ggtggagggt ctggttcatg accgttctgg gagcggatcg actctttata tcgagcctat
     2101 agccctagtg aatttaaaca atgatcttag ggaacttcga agtgatgaga aggaagaaat
     2161 ccacaaaatc ttaaaagaaa ttagtctgcg tctgggacaa gaagaaagca tcattccagc
     2221 ctatatggct acagaagaac tgtatcgggt ttttggcatg gcgaattacg cccttgaaca
     2281 tgatagtcat ctggcaaaaa ccggagaaaa gattcttctt aaaaaagcaa gacatcccct
     2341 tcttgatcaa aaaacagtgg tgcctatgga ccttgatttt ggtgaggagg caaggattct
     2401 tattattacc ggaccaaata cgggaggaaa aacggtagcc ctaaaaaccc tggggctatt
     2461 ttctcttatg catcaatttg gcatggcggt cccctgtgat gatggatcgg tccttccttt
     2521 ttttgatgat atctttgtag atgttggaga tgaacaaagt attgagcagt cactgagtac
     2581 tttttctagc catatggtca atttgatttc aattcttcaa gaggcaggac ctaacagcct
     2641 ggttcttctt gatgaacttg gtgcaggaac tgatccccaa gaaggagcag ccttagctag
     2701 aagtctccta gtagaattcg aggaaaaggg ttctatggta tttgctacca gccactattc
     2761 tgagatcaaa aactttgcct ttaaacggcc tggctatatg aattcttcag tggagtttga
     2821 tgccaagacc ctaagtccaa cctttcgcct gctaatgggt gtaccgggta gctccaatgc
     2881 cctatatatt tctcagcgac tgggattacc agaaagaatt atagaaagag cccgctctag
     2941 tatggatgat cagggcctgg ccatggaaga aatcctagca gaaatagagg aaaaacgtct
     3001 tcatcaagaa gagcttgaac taaaagcaag aaggagttta gaagaagcca aaaaattgga
     3061 agaagaattc gaagagaagc ttcgtcatca gcaagagcaa agggacaggg aactactaaa
     3121 ggctaaaaag gaagcagcca agcttcttaa gcaaactgaa gaagaagcgc gtgatataat
     3181 gaaaaacctt cgtagtatga agggaaaggt agatttttct tctatggaaa agcaatccca
     3241 acgcctgggg caattaaagg aaaatctaca agaccaaaca gaagctagac caataaaagc
     3301 ccctgcaaaa ctaaaggtgg gagaaaagat ctttatcccc catttaaata ctataggcga
     3361 agttcttgat aaacccaata aaaaaggcag ctttaatgcc caggtgggaa ttatgcgcct
     3421 taagcttaat attagccagg ttgaaaaatc gaaggttagc caaaagcaaa tagttaaaaa
     3481 gcaagttcag cagtttgttg ccagtggggt aaagacttct ttggaccttc gaggtaaaaa
     3541 cagtgaagat gcccgggtgg agctagggat ctttattgac caagccctac ttagtaatcc
     3601 tcctgaactt gagattattc atggaaaagg caccggtgta ttaagggatt ttgtaaaaga
     3661 ttacttaaaa aaatccccct ttgtaaaaag ctttcgccaa ggtggacacc atgaaggggg
     3721 aagtggtgta accctggtaa aattacacta gggcacagga aacttttgga gatactcatc
     3781 aatgagctca tcgcgtaaga tgagttcatt tttttgatta taatagtaaa tctttgatag
     3841 ataatagtcc tcgctaccgc gataggagta cattttccca ccagaaaaaa gaaggagaaa
     3901 aagaagcaga actgatataa gctgttttgt tctttttttc accttttgtt tatctgttaa
     3961 tagataaagg gcaaagccac caaaaagacc aaaaacatgt ccagccaggt caatattagt
     4021 gccaaagata gagtaaacaa tattaattgc tagaagaata aagatatctc tgccaaagta
     4081 ttccttgtag cgtgttttat taagaaaata aagataaaga tgaaaagcaa agtaaccata
     4141 gactactcca gaggccccca cggatacttt gggtgagcta agaaaagaac ccaggttagc
     4201 aatgattccc atggcaagac ttaaaaggac aaatcgtttt ggcccatagt agccctcaac
     4261 aaagcgccca aagatataaa tggagaggca gttaaggcca agatgttgcc aggaggcgtg
     4321 aagaaacata ggcataataa gatgccagag tttgccatcg gccaaagaga agttattttg
     4381 agctccaaaa tctattagag ttacaacaga cacagtggga tctattgtat atatcataag
     4441 aaatattaga atattaatcg aaatatataa tttagtagcg gtaaatttct tattcatggt
     4501 cttgacctct tttgctgtgt gtggtaatct ttgtgcaaat agcatagcat aaaaggtcaa
     4561 aagaagaaat cccaaataaa ccaaagcgaa tctgggaagg tggaagccag agggtggatg
     4621 ttggggtagc gcgcttttat tgtgaccgat taatatttga gtgagtctga ctaactagag
     4681 tggtaccgcg gtgaaaacat cgtctctaga tggggacttt ttttattgga ggaaaaatga
     4741 aaaaacttaa aaatgaactt gctttaatcc tagaaccttt tttaaaagag tatctttctt
     4801 tagaggaaat agaagaaact ttggaactgc ccaaattcgc cgaacaaggg gacttagcct
     4861 ttccctgttt taagcttgcc aagagtatga gaaaaagtcc tcatataatt gcccaagaac
     4921 ttagccaaga aataagcagc gataagtttg aagtaaaaaa ccaaggcccc tatataaact
     4981 tttatatcaa tagacaagaa ctagcagaaa ccatacttac agaaatccta agtaaaaaag
     5041 aagactatgg ctccagtgag gaaggacaaa atcgtccagt tattattgag tactcctcgg
     5101 ctaatatttc taaagaactg cactttggac atatacgagg aattatgatt gggtccagcc
     5161 tctataagat tacaaaaacc ctaggttata aggcaatagc catcaatcac ctaggtgatt
     5221 atggcattaa ctttggcaaa attattactg cctataaaca ctggggagat aaagaacata
     5281 tagaaaaaat aggtgttagg ggtttactgg agctctattt gtactttaac aaggcctcag
     5341 cagaagaccc aagttatatg gaagaagcaa gaagatggtt ttataaacta gaagaagaca
     5401 aagaccctga agccttggag ctttggaaat ggtttaagga tgtatctctt aaagaataca
     5461 atagggtcta taagctaata ggttgcactt ttgactctta tgcaggagag gctttttatt
     5521 cggatatgat gcctgatatt agacaggaac ttcttgaaaa agacctaatt attgaagatg
     5581 atggcgctga gattatagat cttagtgatg aaaacctgcc caatgttatt gtaacaacct
     5641 cacaagggtc aagtctttat ataacccgag atatagctgc tgctatttat cgtaaacaag
     5701 aatatgactt ttatagaaac tgctatgtag tgggcagtga gcagcgccta cactttactc
     5761 aattaaaggc cattcttaag aagatgggtt atgattggtg ggatcagtgt gagcatattg
     5821 accatggact tgtaatgatg gaaacaggca agatttctag cagggatggc gataatattt
     5881 ttttagagga agtgattcaa accgccattc aaaaaacaag agatagtata gaagaaagaa
     5941 atccagaact agaagataaa gacgaaatag ccagacaggt tgggctaggt gctctagcct
     6001 atagagaact tagtaatagc cgagtaaaag actatacttt taactgggat gaaaccataa
     6061 gctttgaagg agaaacaggg ccctatcttc agtacaccaa tgttcgttgt cagtctcttc
     6121 ttacaaaggg tgcttatgat gaaagtctag gttttgatgg aagccatcta acagaagatt
     6181 cttccaagga acttttatgg gaactaggac tgcttccttc catccttact agtgcctttg
     6241 aaaagatgga acccgccctt ttaagccgat atctgatggg tgtggccaag ctctttaata
     6301 aattttatgc tcaagttagt gtaattgtag ataataagga agaaaaaaac tccaaactag
     6361 ccctagttaa agcggttagt ac
//
LOCUS       DASRNA010000105         8743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_849616,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000105 DASRNA010000000
VERSION     DASRNA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849616"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..939)
                     /locus_tag="VFD08_02835"
     CDS             complement(55..939)
                     /locus_tag="VFD08_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK09984.1"
                     /translation="MKKSSNGYILAIASALSFSLVSIVGKIIMTRGINPSILIFWQYT
                     AVLIGLALYFYFAKVSIRIETPYILLGGLFSVAANFSFYTSMNLTGAGLATLFIYFSP
                     VFIALFFSLSGIRQITSSGWLAILLALLGSALTLGIFSGIKIQVLGVIAGLFAGLFYA
                     SYNIALDLKLTKINFLIINFYVALQGLILISLFNFTQAIKPWAILPKEILAIVFLGLL
                     GGVLPNFLNFASIRLIGSDKTSVIMSTELPATVLLAFFFLEEKMSLMQILGIVLVLAS
                     VLLLKSQEDPCEESLPER"
     gene            complement(1084..1440)
                     /locus_tag="VFD08_02840"
     CDS             complement(1084..1440)
                     /locus_tag="VFD08_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004681663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HZK09985.1"
                     /translation="MKLPNCPKCKSEYTYEDGNLFVCPMCGKEWSQEKVEVEVEAEAS
                     GEVIRDAHGNPLEEGDDVIVIKDLKVRGSSVPVKKGIKVKDIKLIHNPSDGHDIDCRI
                     DGFGAMKLKSEFVKKA"
     gene            complement(1587..2693)
                     /gene="msrB"
                     /locus_tag="VFD08_02845"
     CDS             complement(1587..2693)
                     /gene="msrB"
                     /locus_tag="VFD08_02845"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZK09986.1"
                     /translation="MKLKYIVIIIVLVLLGALLIRNINRSGEEVMQKEDLNLVGENVE
                     DNLAVVYLAGGCFWGVDAFFDRIEGVESVRSGYANGDTINPTYNDVLYKKTNHAETVE
                     LKYDPAKTDLTNILLYYFKIIDPVSLNRQGNDIGSQYRTGIYYTKEEDLETIKKIVEK
                     EQEKYDKEIVVEVEPIKSFYLAEDYHQDYLAKNPNGYCHIDLSLAEEAIDREEPFEGE
                     ASDPFKKPTDEELREILSQEQYEITQKAGTEAPYSHEYNDLEEKGIYVDIVSGEPLFS
                     SQDKYDAGCGWPSFTKPIDEELIKELKDKSLGMVRTEVKSKEADSHLGHVFTDGPREE
                     GGLRYCINGSALRFVPFEDMQKEGYGYLMDLFED"
     gene            complement(2967..3794)
                     /locus_tag="VFD08_02850"
     CDS             complement(2967..3794)
                     /locus_tag="VFD08_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZK09987.1"
                     /translation="MMGAIMGDIVGSRFEFNNHKSKDFSLFTPDCQVTDDSIMSLSLA
                     KALMETEKRLEARPGGFDQDYYSLLEKMALRYMQEIGRKYPHCGYGGRFARWIFSDKP
                     RPYNSYGNGAAMRISPIGFFAKSEEEVFALSEAVTKVSHNHKEGLKGAEATALAIFMA
                     RQGSSKGQIRDRINRDYYPLDFTIDQIRDTYSFNETCQATVPQAIQAFIESTSFEDAI
                     RTAISLGGDSDTLAAITGSIAQAYYGLPLALEQEALSYLDKQLLEIYLDWTEFLSRV"
     gene            complement(3846..4067)
                     /locus_tag="VFD08_02855"
     CDS             complement(3846..4067)
                     /locus_tag="VFD08_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HZK09988.1"
                     /translation="MAKIKYEIKEKLGTLSQNSRGWSKQLNLISWNDREAKYDLRDWA
                     PENEKMSKGITLSPDELRSLKDLLNRLDI"
     gene            4210..5583
                     /locus_tag="VFD08_02860"
     CDS             4210..5583
                     /locus_tag="VFD08_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZK09989.1"
                     /translation="MIEKKGQRMNLVKIQQNIKESKDQSIETLKEFVRHPSVSRDKEA
                     VEKCAYYLKSLLDKYNIDSKIYPTKGSPLVYGQVNSPNKNAKTVLFYGHYDVQPPEPL
                     EDWHTPPFEPSIRKGRLYGRGAADNKGQLLAHILAVKSILEIEKTLPINVKFIFEGEE
                     EIGSGSLGPFVKENKDLLDCDLVITSDGPMHDSGAPILAFGVRGVMNFQLELKTAQTD
                     NHSGNKGGVIPNPAWELIELLTTMKDKNDLVTIEGFYDDVLKPSKKELTLIQALPFDP
                     EDLAKVYGVEKIELDKESFYKRLMLLPTLSINGLISGYTGSGTKNIIPNKALAKMEVR
                     MVKNQNPDDLFEKITNHIKKVNPNVTIISQEEDMYPSRTSLELDISQKIIRAVKKAHK
                     TDPLVFPSMGGSLPDYIWTKILGKPSIMVPYANADEANHAPNENMDLTCFINGIHTSV
                     HIINELK"
     gene            5639..6745
                     /locus_tag="VFD08_02865"
     CDS             5639..6745
                     /locus_tag="VFD08_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZK09990.1"
                     /translation="MDTDKLRALFPLTENYTFLNNAAESPLNMAVKNKLDYYLDLALR
                     APHTKPSVRYQVKKKLAKLLGGNPSDYALMTSTGMGVSIIAAGYKWTKGDNVVLPSNE
                     HWNNSFPWQALEEKGVEVRFVEPDQQNRILPEKIAALTDKKTKIVALAAVSFNTGFRS
                     DLKEISQIAHKQGALFLVDGIQAAGVVPLDVEKDHIDILCCAGFKWLLGLPGTGFVYL
                     NKRAQEKINPLLPGMFAADLYLKELTYHPDARRFETGSIAYSLFYAWTAGLELLMDIG
                     VENIHKKILLLTDLIISGLEEKDIEIITPVEHISERSAIILFTLGSLEKNKALYEKLL
                     AENIILTLRDNLLRISPSFYNTEEEIKSFLSFLA"
     gene            complement(6840..8156)
                     /locus_tag="VFD08_02870"
     CDS             complement(6840..8156)
                     /locus_tag="VFD08_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZK09991.1"
                     /translation="MAVGSLFIVLFILLMIGVPVGFAIGGATMFSMALFSDINMAINA
                     QYAYSGIFSFTVMAIPFFMLAGLIMSTGGIARKIVNFAATLISFVNGAIASVTIISCM
                     FFGALSGSGMATTSAIGGMMIPEMKKKGYDPAYAATVVCFGGIVGPIIPPSLSFVLYG
                     ASTGVSIPQLFLAGIIPGILLGVLFVITNTIICTINGTDLKSKRQTEGDEKVPIGKAM
                     KYRLSEVWASFKDGFWALLSPVIILGGIYSGVFTPTEAACVSVVYSIIISLFVYKDLD
                     LAGLWDVFLDTAVLNGITSFLLGYSTVFSTFMTYEKVPQMITNFLTNVSTNPVVVLLF
                     INLILLVVGLFLDTVPAIIVIAPILMPTVHALGINPIHFGVIMAVNLAIGLCTPPYGC
                     NLFVGAAVAKIKLESMFKHIVPYFIVSLVGLALITYIPWLSLAFIK"
     gene            complement(8156..8659)
                     /locus_tag="VFD08_02875"
     CDS             complement(8156..8659)
                     /locus_tag="VFD08_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZK09992.1"
                     /translation="MKNKIVKTLNKIEDTALVAMFVAMVAVIFFQVVMRYVFNNSSAW
                     SEELGKFLFVWLSWIGISIGHRKREHITITIVVDRLSKKAQLVLEAISELVLITICGI
                     TLFYGVKMIGIQITTPYAGIKISTAWGYLSLVLGCGIFILRAAVYFIEAIKAIMGKGP
                     ITESGDL"
BASE COUNT         2806 a         1874 c         1539 g         2524 t
ORIGIN      
        1 gtctctatat attttacctt ggtaaaaatc atacttcttt ctttcatttt tattttacct
       61 ctcaggcaaa gactcctcac aggggtcttc ctggctttta agaagtagaa cagaagctag
      121 aaccaaaaca attcctaaaa tttgcataag gctcatcttt tcttctaaaa agaaaaaggc
      181 taaaagcact gtggctggaa gctctgtact cattataaca gaggtcttat ccgaaccaat
      241 aaggcgaata gaggcaaagt ttaaaaaatt aggcaaaact ccccctaaaa ggcctaaaaa
      301 tacaatggcc aagatctcct tgggcaaaat agcccagggt ttaatagctt gagtaaaatt
      361 aaaaaggcta ataagaatca ggccctgaag ggccacataa aagttgataa taagaaagtt
      421 tatcttggtt aattttaaat ccaaggcaat attgtaagaa gcataaaaaa ggcccgcaaa
      481 aagacccgca ataacaccca gcacttggat cttgatgcct gagaaaatcc ccaaggtaag
      541 ggcactgcca aggagggcta aaagtatagc cagccaacct gagctagtga tttgacgtat
      601 cccactaaga gaaaaaaaca gggcaataaa aaccggagaa aaatagataa acaaggttgc
      661 aaggccagct cctgttagat tcatagaggt gtaaaagcta aaattagctg caacgctaaa
      721 tagaccccct aaaagaatat agggagtttc aatacgaata gatactttag caaaataaaa
      781 atataaggcc aggccaatca aaaccgcagt atattgccaa aatatcaaaa tactgggatt
      841 aatcccccta gtcataataa tttttcctac aatggacaca agagaaaaac tcagggccga
      901 tgcaatagct aaaatataac cattacttga ttttttcatt tcttcctcac ttcagccttt
      961 tttattaaaa aggagctcac tagcaagata taccttttcc taaactagct tacaagccct
     1021 aggatgaatc aatattaaaa aacaggctac ccctgccaag ggacccagcc tgcatggata
     1081 gaactatgcc ttttttacaa attcggattt tagtttcatg gcaccaaagc catctattct
     1141 gcaatcaata tcatgcccat cgctaggatt atgtattagc tttatatctt ttaccttgat
     1201 tccttttttt actggcactg agctgcctct tactttaagg tccttgatta caattacatc
     1261 atcgccttct tctaagggat ttccatgggc atctcgaata acttcaccac tagcttctgc
     1321 ttctacttcc acttcgactt tttcctggct ccactctttg ccacacatag gacagacaaa
     1381 aagatttcca tcttcgtaag tgtattctga tttgcatttt ggacaattgg gtaatttcat
     1441 caatcttttc acctccatta ttttcattcc cccttatagt atcatattta gccctagagc
     1501 tctaatttgt acactttttg atacaattaa aaagagcttc tctgctaaga aaaaaggacg
     1561 ggcaaaccgt ccttttcatc tcttctttaa tcttcaaata ggtccattaa atagccatag
     1621 ccttcttttt gcatatcttc aaaggggaca aagcgaaggg cacttccgtt aatacagtat
     1681 cttagaccac cctcttctct aggaccatca gtaaacacgt gccctaaatg agaatctgcc
     1741 tccttgcttt tgacctcggt tctaaccatg cccagggact tgtcctttaa ttcctttatt
     1801 aattcttcat cgattggttt ggtaaagctt ggccaaccac atcctgcatc atatttgtct
     1861 tgggaggaaa aaagtggctc tcccgagaca atatcaacat agatcccttt ttcttctaag
     1921 tcattatact catgtgagta gggggcttcc gtccctgcct tttgggttat ttcatattgc
     1981 tcctggctta agatttctct taactcctca tccgtgggct ttttaaaggg gtcactagcc
     2041 tccccctcaa aaggctcctc ccggtcaatg gcctcctcag ctaaacttaa atcgatatgg
     2101 caataaccat taggattttt agctagatag tcctggtggt agtcttcggc caagtaaaaa
     2161 cttttaattg gctctacttc gaccacaatt tctttatcat atttttcttg ttctttttct
     2221 actattttct ttatggtttc tagatcttct tcctttgtat agtagatgcc ggttctatac
     2281 tggctaccta tatcatttcc ttgcctattt aaggagaccg gatctattat tttaaagtag
     2341 tataagagga tattggttag gtctgtttta gctggatcat actttagctc tactgtttct
     2401 gcgtgatttg tttttttgta taggacatca ttataggtgg gattgatggt atctccattg
     2461 gcatagcctg aacgaacact ttctacccct tctattctat caaaaaaagc atctacgccc
     2521 caaaaacagc ccccggccaa atagactact gctagattgt cctctacatt ttcccctaca
     2581 agatttaaat cttctttttg cattacttcc tccccacttc tatttatatt tcttatcaat
     2641 aaggctccta gtaagactag cactattata ataacaatat atttaagctt cacattacaa
     2701 ctcctttgcc tctataaaga aagtccaatt accggtgttt ttctttgtaa aaggatatcc
     2761 tggtcaagat aatacaccct gccctataga acaattttac ccctagagac tggctgcaaa
     2821 caatttcacc taactgctta acgttttttc tctgggactg atctaccata aatagacaaa
     2881 agatgaaatt aggataaaaa aagacctatg cttttattat taacataagt ctaaaattca
     2941 ctgtgaaaag acctctttgg ctgcccctag accctgctaa gaaactctgt ccagtcaagg
     3001 taaatttcta gcagctgctt atctaagtaa gaaagcgcct cttgctcaag tgcaagggga
     3061 aggccgtaat aggcctgggc aatggatcct gtgattgcag ccagagtatc gctatctccc
     3121 cccagggaga tggcggttct tattgcatcc tcaaaggaag tagattcgat aaaggcttgt
     3181 atggcctggg gaacggtagc ttggcaggtt tcattaaagc tatatgtgtc tcttatttgg
     3241 tcgatggtaa aatcaagagg ataataatcc ctatttatcc tatctctaat ctgcccttta
     3301 gaagatccct gccgggccat aaaaatagcc agcgctgtcg cctcagcccc ttttagcccc
     3361 tccttgtgat tatggctaac ctttgtaacc gcttcagaca gggcaaaaac ttcttcttca
     3421 cttttggcaa aaaaaccaat aggacttatt ctcatggccg caccatttcc atagctatta
     3481 tagggccttg gcttatcact aaatatccat cgcgcaaatc tacctccata gccacaatgg
     3541 gggtatttgc gtccaatctc ctgcatatat cgaagggcca ttttttctag caaggagtag
     3601 tagtcctgat caaagccgcc cggcctagct tccagtcttt tttcggtctc cataagggcc
     3661 tttgcaagac tcaggctcat aatactgtca tcggtcacct ggcagtctgg ggtaaataag
     3721 ctaaagtcct tactcttgtg attgttaaat tcaaaccggg aacctacaat atctcccata
     3781 attgcaccca tcataaagcg cctccttaaa tggattccac taaatcttct tttatttata
     3841 agaaatcata tatccagcct attaagtaga tctttaaggc ttctgagctc atcaggactt
     3901 aaggtaattc ccttactcat cttttcattt tctggagccc agtccctaag gtcgtattta
     3961 gcctctctat cattccagct aattaaattt agttgctttg accatcccct actattctgc
     4021 gaaagagtcc ctagcttttc ttttatttca tactttatct ttgccatgta aatttttcct
     4081 ttcataaata tagataagtt ccatagcttt attatatact ataaaatacc agtaaaactt
     4141 tatgctccaa aatatccaag ataaaactgc ctggtctttt agtggtagaa taaaagtagc
     4201 aggaaaagga tgatagaaaa gaaaggacaa aggatgaacc tggtaaaaat tcagcaaaac
     4261 attaaagaaa gcaaagacca aagtatagaa actctaaagg aatttgtaag acatcccagt
     4321 gtaagtaggg acaaggaggc agtagaaaaa tgtgcctact acttaaagtc gctattggat
     4381 aagtacaata tcgacagtaa aatttaccct accaagggaa gtcccctggt ctatggacag
     4441 gtgaattcac ccaataaaaa tgctaagact gtcttatttt atggccacta cgatgtccag
     4501 cctcctgagc ccttagaaga ctggcatact cccccctttg aacccagcat aagaaagggt
     4561 aggctctacg gtagaggggc cgctgataat aagggccagc ttttagccca tattctggct
     4621 gttaaaagca ttctggaaat agaaaaaacc ctgcccatta atgtaaagtt tatctttgaa
     4681 ggagaagaag aaatcggcag tgggtcttta ggaccatttg ttaaggaaaa caaagacctc
     4741 ctggattgcg atcttgttat aacatctgat ggccctatgc atgatagtgg ggcccccatc
     4801 ctggcctttg gagtaagggg ggttatgaat tttcagctag aactaaaaac tgcccagact
     4861 gataatcatt cgggcaataa gggtggggta atccccaatc ccgcctggga actaattgaa
     4921 ctccttacta caatgaagga taaaaatgat ctagtaacca tcgagggctt ttatgatgat
     4981 gtgctaaaac caagcaaaaa agagctcact ttaatccaag ccctgccctt tgatccggaa
     5041 gatcttgcta aggtatatgg ggtagaaaag atcgagctgg ataaagaaag cttttataag
     5101 agacttatgc tcctacccac cctgagcatc aatggcctaa tcagcggtta cactggcagt
     5161 ggtacaaaaa atattattcc aaataaagcc ctggcaaaaa tggaagtaag aatggttaaa
     5221 aatcaaaatc cagatgattt gtttgaaaaa attacaaacc atataaaaaa agttaatcca
     5281 aatgtaacaa taatcagcca ggaagaggat atgtatccat caagaacctc cttggaacta
     5341 gatatttctc aaaaaataat aagggctgtt aaaaaagctc ataagacaga ccctcttgtt
     5401 tttccctcca tgggaggaag cctacccgac tatatatgga ccaagatttt aggaaaaccc
     5461 tccatcatgg tgccctatgc caatgcagat gaggcaaatc atgcccccaa tgaaaatatg
     5521 gacctaactt gttttatcaa tggaattcac acaagtgtgc atataataaa tgaactaaaa
     5581 taagtcaaac cctagagcca aatagaccta gactttctaa aaaaaaatgg aggtatttat
     5641 ggatactgat aaactaagag ccctttttcc cctaacagaa aactatactt ttctcaacaa
     5701 tgccgctgaa tcaccactaa atatggcggt aaaaaacaag ttagattact atcttgatct
     5761 agcactaagg gctccccata caaaaccctc cgtgcgctac caggtcaaaa aaaagctggc
     5821 taaattacta gggggaaatc ccagcgacta cgccctgatg accagtactg gaatgggagt
     5881 tagtattata gcagcaggat ataagtggac caagggcgac aatgtggtgc ttccctctaa
     5941 tgaacattgg aataatagct ttccctggca ggccctggaa gaaaaaggag tggaagtgcg
     6001 ctttgtagag ccagatcagc aaaatcgaat cctgccagaa aagattgccg ctcttacgga
     6061 taaaaagaca aaaattgttg cactagcagc agtaagcttt aatacaggct ttcgctcgga
     6121 tctaaaagaa atctcccaaa tagcccataa gcagggcgcg ctctttcttg ttgatggcat
     6181 acaggcagct ggagtcgtcc cccttgatgt tgaaaaagac catattgata tcctatgctg
     6241 cgccggcttt aaatggctgc taggtctgcc tggtacgggc tttgtctatc taaataagag
     6301 ggcgcaagaa aaaatcaatc ccctcctgcc tgggatgttt gcagcagatc tttacctgaa
     6361 ggaacttacc tatcatcccg atgccaggcg ctttgaaacc ggttctattg cctactcact
     6421 tttctatgca tggacagctg gcctcgagct cctaatggat ataggagtag aaaatatcca
     6481 taaaaagata ctgctgctta cagatcttat catttcaggc ctggaagaaa aagatataga
     6541 gataatcaca ccggtagaac atatctcaga gcgctcggct attatcctat ttactctggg
     6601 ttctttagaa aaaaacaagg ccctatacga aaagctacta gcagaaaata taatcctaac
     6661 cctaagagat aaccttctta gaatcagccc ttctttttat aatacagaag aagaaatcaa
     6721 aagttttctt agctttttag cttgagcatt tttattctta tggctcctaa aatgccccct
     6781 aatccactta tggataaggg ggcatttatt aactactgct ttatattttg tgcttttttc
     6841 tattttataa aggctagaga taaccagggt atataggtta ttagagcaag cccaaccaaa
     6901 gatactataa agtaaggaac aatatgttta aacatacttt ctagttttat ctttgccacc
     6961 gcagctccaa caaatagatt acatccatag ggcggtgtac acaaaccaat ggccaagttt
     7021 actgccataa taacaccaaa gtgaatgggg ttaattccca gcgcatgcac cgttggcata
     7081 agtattggtg caataacaat aatggcagga acggtatcta aaaatagtcc cacaactaaa
     7141 agtataaggt ttataaataa caagacgacc actgggttgg ttgatacatt agttaggaaa
     7201 ttggtaatca tttgtggtac tttttcatag gtcataaagg tagagaaaac cgttgaataa
     7261 cccaataaaa atgatgtaat cccattaagt actgctgtat ctaaaaacac gtcccataaa
     7321 cccgcaagat ctaagtcttt ataaacaaac aaacttatga taatagaata tacaaccgaa
     7381 acacaagctg cttctgttgg ggtgaaaaca cccgagtaaa ttccacctaa gataataact
     7441 ggagataaaa gggcccaaaa tccatcttta aagctggccc aaacttcgga aagtctgtat
     7501 ttcatggcct ttccaattgg aaccttttca tcgccctcag tttgtctttt tgactttaag
     7561 tctgttccgt tgattgtaca aataatggtg tttgtaatga caaataatac tccaagaaga
     7621 atcccaggga taatacctgc taaaaacaat tgaggaatag atacccccgt tgaagcaccg
     7681 tataatacaa aggataaact aggaggaata atgggtccaa caataccacc aaaacaaaca
     7741 acagtagcag cataagccgg atcatatccc ttctttttca tctcaggaat catcattcca
     7801 ccaattgctg aagtagttgc cattccagaa ccagataatg ccccaaaaaa catacaagat
     7861 ataatagtta cactagctat ggcgccatta acaaaactaa ttagagttgc agcaaaatta
     7921 acgattttcc ttgcaattcc acctgttgac ataataagtc cagctagcat gaaaaacgga
     7981 atagccataa cagtaaaaga aaagattcca ctatatgcat attgagcgtt gattgccata
     8041 tttatatctg agaatagagc cattgaaaac atggttgctc caccaatagc aaatccaaca
     8101 gggaccccta tcattaacag tataaataat acgataaata aactacctac tgccattata
     8161 aatccccact ttctgttata ggtcctttac ccattatagc ctttatagct tcgataaagt
     8221 agaccgctgc tctgagtata aatatgccac aacccaagac cagtgataaa taaccccaag
     8281 ctgtactgat tttgattcct gcataggggg tggtgatttg aatcccaatc attttaacgc
     8341 cataaaataa ggtgattcca catatggtaa ttagaaccag ctcagaaata gcttctaaaa
     8401 cgagttgggc cttttttgag agtctatcta ctactatggt aatggttata tgttctcttt
     8461 ttctatggcc tatgcttatc ccgatccagg ataaccatac aaataagaac ttaccaagtt
     8521 cttctgacca agcagatgag ttattaaaga catatctcat aacaacttga aaaaagatta
     8581 ctgcaaccat tgcaacaaac atagctacaa gggctgtatc ttcgatttta ttcaatgttt
     8641 ttacaatttt atttttcata tcacatcatc atatctgcaa gagatattaa ggtctctctc
     8701 ctttcaaata taataagttt attagtaata tgtaattcaa gga
//
LOCUS       DASRNA010000106         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_32405,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000106 DASRNA010000000
VERSION     DASRNA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32405"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2118
                     /locus_tag="VFD08_02880"
     CDS             <1..>2118
                     /locus_tag="VFD08_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZK09993.1"
                     /translation="GYVWHTTGSGKTITSFKTALFLSTRGGFDKVIFLVDRRELDKKT
                     SENFKAYSAYESVSVDDTSHTYQLKKQLLSGKRGIVVTTTFKLNNLVKKLMEGADFGL
                     SEKKFIFIIDEAHRTTMGEMMVNIKRFFRKNGLFYGYTGTPLFDENKVIGMVNEKSEI
                     IDTTEKLFGPGLHKYTIDQAIADGNVLGFNVDYINTGEFISYGDLREQIKAQIEAEKP
                     DMPGLEIERLVYGWSDLEVEKEAVKRNLLFYQDETHIPKVVERILDNWEEQSKDRVFN
                     AILTAAYKKRAIAFFNEFKVQLRRRGDNINLALTFSFGSESETDKVEPEIIEMMFKDY
                     ASFTGIEFVSGDKSKGEEAYFEDIVARGTRGGSGRNERNIDLIIVADQLLTGYDSKYL
                     NTLYVDRPLALQGLIQAYSRTNRIYGKEKEFGSIVNFQYPKITEEQVNTALTLYGSGG
                     ASSRAIVENYDTAVEKLSIKVEEMIKSLEDPSQWESLNLDEKAKELFVSSFVDASKQL
                     GTVMQYYEYKWDNERFKIDEHTWLKYVGAYKNLTYKERNPIDEPIIPLSGKTKITTRQ
                     VIDDKHILGLIGSRIRNEKGIQTIDDETLRLIYEEIQELSDMGQAKQAKLLKEFVEKE
                     LITGHLKADLDFDQAFENWKKRRLQMEAEKFAKEWGLYSDVLLKSLDQYSIVKEDAIP
                     YIDEIQLSIDYEKAENKESGSQLP"
BASE COUNT          775 a          313 c          483 g          547 t
ORIGIN      
        1 ggctatgtat ggcatactac cggttcaggg aagacaatca ctagttttaa aacagctttg
       61 ttcttatcca cccggggcgg ctttgacaaa gtcatttttc tggtggatag aagggaatta
      121 gataaaaaga ctagtgaaaa ctttaaagca tactccgctt atgagtcggt ttccgttgat
      181 gatactagtc atacatacca actaaagaaa cagctgttgt ccggaaaaag aggcattgtt
      241 gtaaccacca catttaagct gaataattta gttaaaaagc tgatggaggg agcagatttt
      301 ggcttatcag agaagaaatt tatctttata atagatgaag cccatagaac aactatgggg
      361 gagatgatgg tcaatataaa aaggtttttc aggaaaaacg gactttttta tggatacaca
      421 ggcacgccat tatttgatga aaataaggtt attggtatgg ttaatgaaaa atcggaaatc
      481 atagatacca cagaaaagct ttttgggccg gggcttcaca aatatacaat agatcaggct
      541 atcgcagatg gcaatgtgtt agggtttaat gttgactata tcaataccgg tgaatttata
      601 agctatgggg atcttagaga gcaaataaag gcacagatag aggccgaaaa accggatatg
      661 ccagggctgg aaatagaaag acttgtctat ggttggagcg acttagaagt tgaaaaagaa
      721 gcggtaaagc gaaatctgct tttttatcag gatgaaactc atatacctaa agttgtagaa
      781 agaatattgg ataattggga agagcagtct aaagataggg tatttaacgc tattttaaca
      841 gcagcctaca agaaaagggc aattgccttc tttaatgaat ttaaagtaca acttagaaga
      901 agaggcgata atattaacct tgccctaact tttagctttg gcagtgaaag tgaaactgac
      961 aaggtagagc ccgagataat cgaaatgatg ttcaaagact atgcaagttt tacaggtatt
     1021 gaatttgtta gtggcgataa atctaaaggt gaagaggcat actttgagga catcgtagca
     1081 agaggcaccc gtggaggcag tggcagaaac gaaagaaata tagaccttat catagttgcg
     1141 gaccagctgt tgacaggcta tgactccaaa tatttaaaca ccctttatgt agataggcca
     1201 ttggcattgc aggggctgat tcaagcatac tccaggacta acaggatata tggtaaagaa
     1261 aaggaatttg gatctatagt aaattttcaa tacccgaaaa taacagagga acaggtaaac
     1321 accgctttga cgctatacgg aagcggtggt gctagctcaa gagctattgt agaaaactat
     1381 gatacggcag tagaaaaact atctataaaa gttgaagaaa tgattaagtc attagaagac
     1441 ccaagtcagt gggagagcct aaatttagac gagaaggcca aggaattatt tgtgtcttcc
     1501 tttgtggatg cgagtaaaca attaggtact gtcatgcaat actacgagta taaatgggac
     1561 aatgaaagat ttaaaataga cgaacacacc tggttaaaat atgtgggcgc ttataaaaac
     1621 ttaacatata aggaacggaa cccgatagat gagccgataa tccctttaag cggtaagacg
     1681 aaaattacaa ccaggcaggt aatagacgat aagcacatat taggtttaat cggatcaaga
     1741 attagaaatg agaagggtat ccaaacaatc gatgatgaaa ctttaaggct aatctatgag
     1801 gaaatccagg aattaagtga tatggggcag gcaaagcagg ctaaattatt gaaagaattt
     1861 gttgaaaaag aattaatcac cggacatcta aaagcagact tagactttga ccaggctttt
     1921 gaaaattgga aaaagcgaag gttacaaatg gaagctgaaa aattcgctaa ggaatggggt
     1981 ttatatagtg atgttttact taaatctttg gaccaatatt ctatagttaa agaagatgct
     2041 atcccatata tagacgaaat acaattaagc attgactatg aaaaggcaga gaataaagaa
     2101 tcaggttctc agttacca
//
LOCUS       DASRNA010000107         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_32975,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000107 DASRNA010000000
VERSION     DASRNA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32975"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFD08_02885"
     CDS             <1..228
                     /locus_tag="VFD08_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09994.1"
                     /translation="NVNASKESKALSLGEIKALDPDFILILGETSQARREELSELGAV
                     KENNLYFLGEYPRLAGSNFVSVLENLIKDMK"
     gene            239..1642
                     /locus_tag="VFD08_02890"
     CDS             239..1642
                     /locus_tag="VFD08_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZK09995.1"
                     /translation="MEKWLELDLQKYWQLQEEGKLTSRELCAWYLERIARYDGHYRTI
                     LEINPDALFIAEAMDRERKLGKKRSILHGVPILLKDSISTYDGLHTSAGSLALKDHFA
                     KDDAFIVKRLREAGAVILGKSNMTELSHYTSNKMKNGYSARGGQVLHPYNEKSSPSGS
                     STGSSVAVSLNFSLLALGTETKGSVIWPSHNSSIVGIKPSRGLVSRSGIIPICRAHDT
                     AGPMARTVADCAHLLGALSGEDPKDPSTWALDDKRGDYAGFLEKEALKGMRLGIRKTN
                     FGPEEEKLFEEAVKTMEDLGAIFKGPYDLPEVGRDDLVILAHEFKRSFDHYLHEYSGS
                     KKLLEEIVLYNKENTDLIYGQDLLEGALEIGDRLLDSDYLDKRINLAQDKRQEIEDLM
                     DKEELDLIVFPGRSDLPSVSGLPCIVVPCGYTRDNRPLGISFIARNFDEGLMIAGAYS
                     FEQASVKRKPPLVKEEK"
     gene            1639..1977
                     /locus_tag="VFD08_02895"
     CDS             1639..1977
                     /locus_tag="VFD08_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK09996.1"
                     /translation="MKKILFYAMDGEKMCFLHVLMNALDLHKEGMEVAVIFEGASVKL
                     PPLLEEEKNKLYLQAKEEGLLAGICLACSSVLGVLEENKKTGLPLLNDMMGHAGIKPY
                     LLEGYEVISF"
     gene            2660..2974
                     /locus_tag="VFD08_02900"
     CDS             2660..2974
                     /locus_tag="VFD08_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HZK09997.1"
                     /translation="MIKTNQETVDEIKKIIVENEQEGTSVRIYLAGMGCSGPSFGLSL
                     DDKKEEDESWVVEGQEFVMQKDFFSEFGSFTIEYQDGGYIVEPEQLPEGMGGCASCTG
                     CN"
BASE COUNT         1013 a          495 c          693 g          908 t
ORIGIN      
        1 aatgtgaatg catcaaaaga aagtaaggcc cttagcctag gggaaataaa agccctagat
       61 ccagatttta ttttaatcct aggagaaact tcccaggccc gcagggagga actttcagaa
      121 ttgggagcag tcaaagaaaa caatttgtat tttttggggg aataccctag actagctggc
      181 tcgaactttg tgagtgtatt ggaaaatttg ataaaagata tgaaatgagg tcaaataaat
      241 ggaaaaatgg ttagaacttg atttgcaaaa atattggcaa cttcaggaag aaggaaaact
      301 aacaagccga gagctttgcg cttggtatct tgagcgcatt gcccgctatg atggccatta
      361 caggactatt ttggagataa accccgatgc tctttttatt gcagaagcca tggatagaga
      421 acgtaagttg ggaaaaaaaa gaagtattct tcatggtgtt cctattttgt taaaggatag
      481 tataagtacc tatgatgggc ttcacacttc agcaggttcc ctggctctta aggatcattt
      541 tgcaaaagac gatgccttta ttgttaagcg tttaagagag gccggggctg ttatattagg
      601 taaatctaat atgacagaac tttctcacta cacatcaaac aaaatgaaaa acggatattc
      661 cgctagagga ggtcaggttc ttcaccctta taatgaaaaa agctctccct cgggatcatc
      721 aacaggttca tcggtagctg tttctttgaa tttctctctt ttagctctag gcacagagac
      781 caagggatct gttatatggc cctcacataa tagttctatt gttgggatta agccctcaag
      841 agggcttgtt agtcgaagtg gtattatacc tatttgtagg gctcatgata cggctggtcc
      901 tatggctaga acagtagcgg attgcgcaca tctacttggg gccctatccg gggaagatcc
      961 taaagatcca tcaacctggg ctcttgatga taagcgcggg gattatgctg gcttcttaga
     1021 aaaagaagca cttaagggca tgcgtctagg aattagaaaa acaaactttg ggccagaaga
     1081 agaaaagcta tttgaagaag cagtgaaaac aatggaagac ttaggagcca tttttaaggg
     1141 tccctatgat cttcccgaag tgggaaggga tgatctagtg attctagctc atgaattcaa
     1201 gaggagtttt gatcattatc ttcatgaata cagtggatca aaaaaacttc tggaagaaat
     1261 tgttctatat aataaagaaa acaccgactt aatctatggg caggatcttc tagaaggagc
     1321 ccttgagata ggtgatcgct tactggattc tgattatttg gataaaagaa taaatcttgc
     1381 ccaagataaa agacaagaga tagaagatct tatggataaa gaagaattag atttaattgt
     1441 atttcctgga agaagtgatc ttccctcggt tagtggacta ccatgtattg ttgtaccttg
     1501 tggttataca agagataata gacccctagg catatcattt atagctagaa acttcgatga
     1561 aggtcttatg attgctgggg cttacagctt tgaacaagcc agtgtaaaaa ggaaaccacc
     1621 gctggtaaag gaggaaaaat gaaaaaaata ctattctatg ctatggacgg ggaaaaaatg
     1681 tgctttttac atgtattaat gaacgctctc gatcttcata aagaaggtat ggaagtggcg
     1741 gtaatctttg agggcgcttc ggttaaactc cccccccttc tagaagaaga aaaaaacaag
     1801 ctttacttac aggccaaaga agaaggtctt cttgcaggga tttgccttgc ctgttctagt
     1861 gtcctggggg tgctagaaga aaataaaaaa accggtcttc cgcttttaaa cgatatgatg
     1921 ggtcatgccg gtattaaacc ttatctacta gaaggatatg aagttataag cttttagggg
     1981 gtcattgacc ccttttttta ttagctgcta gtctcataat aaaaattatt ataaaaaaag
     2041 gtgctagcat ggggaataaa aatagcaaaa atattaagac aaggacacta aagggttgag
     2101 gggatttttt acccttgggg ttctgccttt tttgggaagt ttgcgttttt tggacagtct
     2161 gctctttttt ggccgcagca taatgttcgt aatccttcaa gggactattt ttctggatat
     2221 ttaaatcata acgatattct tggccgcagc gagggcaaaa gagctggctg tctttcattt
     2281 ttttcttgca ataagaacaa gttttcattt gaattccttt gtactatatt tttcggaaga
     2341 tttcttcttt aagtatataa cattggggtt tttcccgcaa gagtgggtat ctatactacg
     2401 attaagaata attagccctt tgactcagca gagtaaggat cactcatttg ctttataaag
     2461 gaaaaagata ctctgagatt aaaagcaata atcagcgaaa tagccatctt tttctatgat
     2521 aaaaagttac taattaagac ttgtctttgc caagtaaaaa actatatcta taaagaaaat
     2581 gataaacaca atccctacta atctatggta tattaagtaa gaaaacaaag ggaagcaatc
     2641 ccaatataag gagagtatta tgattaaaac aaaccaagaa actgtggatg aaataaagaa
     2701 gatcattgta gaaaatgaac aagaaggaac gagtgttcga atttatttgg ctggcatggg
     2761 atgttcaggt cctagctttg gtttatcttt agatgataaa aaagaagaag atgaaagctg
     2821 ggttgttgaa ggccaagaat ttgttatgca aaaagatttc tttagtgaat ttggcagttt
     2881 cacaatagag tatcaagacg gtggatacat tgtggagcca gagcaattac ctgagggtat
     2941 gggtggatgt gctagctgta caggatgtaa ttaaaagaaa agaagggtag cccttctttt
     3001 ttttgtgtat atgtcaaaaa gcattaattg tgaaataaaa aaagaggtta aacctctttt
     3061 ttttggtgtt gatgagtgga ctcgaaccac cgaccccctg catgtcaag
//
LOCUS       DASRNA010000108         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_699306,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000108 DASRNA010000000
VERSION     DASRNA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699306"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VFD08_02905"
     CDS             <1..145
                     /locus_tag="VFD08_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZK09998.1"
                     /translation="FKELNVIGMDIVEVAPPLDPSLNALFAARKIVTESWGHWYRKNK
                     GFK"
     gene            complement(227..2164)
                     /locus_tag="VFD08_02910"
     CDS             complement(227..2164)
                     /locus_tag="VFD08_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK09999.1"
                     /translation="MNQFDEQKLEKFYDGKLVKKLAKYTKGLWLLLLMSALLLLAGTL
                     FDLLQPRIISTIIDDYLVPRQSYLQEEPQGFALEEGNFSISKEPTSVVLKDQNLEKDG
                     LSRSLSQEENRAYKDYRYKKIINLTLFLLALSILTYLSAMLEQIALNYVGQRVIQRIR
                     SDLFAKLERLSLSFHEKNPVGRLVTRMTNDLGNINELYTNVIVTVLSDVAIILGSIFM
                     MFSMNVKLSFISLSIMPLLVVVTLIFQRKVRLAYRDVRVKLAKINTTLNENLSGMKTI
                     QIFNQEKKFIENFAEDNEKYHVASKGELQIYAIFRPFINLLYYTSLILVIIFGGKFAI
                     EGSIEVGVVIAFTIYVQKLFRPIQDLSEKFTIFQSAMASIERVFMLLEEEETIVNDPK
                     LNPQHLKGDVEFKNVCFSYEKDETVLKNVSFQVKAGETIAFVGATGSGKTTIMSLLTR
                     LYDIDQGEILIDGAPIEDYDKYFLRSRIVPVLQDVFLFSGDIRNNIALLNKDISDERI
                     WEAAEFVNAAHFIRKFPKGLDHHVTEGGSTLSQGERQLLSFARALVHRPDILILDEAT
                     ASIDTQTELLIQDAIEKVVEGRTTFVVAHRLSTIRGADKIIVMHKGAIAEMGTHDELL
                     EEKGLYADLHRLQYAVQEVQE"
     gene            complement(2151..>2757)
                     /locus_tag="VFD08_02915"
     CDS             complement(2151..>2757)
                     /locus_tag="VFD08_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10000.1"
                     /translation="LYNIPRGKIFLGKRDINDLSIEDTRNLIAMVPQDNFLFSTEIEN
                     NIALSDTRPRKDKILQAAKDAHVHEEIESFPQSYQTFLGEKGVNLSGGQKQRTSIARA
                     LYKNAPILILDDSLSAVDTKTEEAILRKLRVDLASHSSIIISHRISTLQHLDEIIFID
                     EGRIVERGNHEELLALRGRYYQVYEKQLLEDKIQGAGYEPI"
BASE COUNT          785 a          537 c          566 g          869 t
ORIGIN      
        1 atttaaggaa ctcaatgtaa ttgggatgga tattgtagag gtggctccac ctctagatcc
       61 ctccttaaac gccttatttg cagctagaaa gatagttact gaaagctggg gacactggta
      121 tcgcaaaaac aagggcttta agtaaagaag aaggcaaaag agaaatctta ttactatggt
      181 ataaaagtaa aagggcaaac cactaaggat ttgccctttt ttgcttttac tcctgtactt
      241 cctggaccgc atactgaagc ctatgaagat cggcatagag acctttttct tcaaggagct
      301 catcatgggt gcccatttca gcaatagcgc ctttatgcat aacaatgatt ttatcagccc
      361 ctcttatagt agagagtcgg tgggccacaa caaaggtagt gcgaccctca actacttttt
      421 caatggcgtc ttgaataaga agttctgttt gagtatcaat gctggctgtt gcctcatcaa
      481 ggatcagaat atctggtcta tgcaccaggg cgcgggcaaa gcttaggagt tgcctttcac
      541 cttgagacag ggtagagcca ccctctgtaa catggtggtc tagtcccttg gggaactttc
      601 gtataaagtg ggcagcattt acaaattctg ccgcctccca tattcgctca tcactgatat
      661 ccttattaag tagggcgata ttgtttctaa tatccccaga aaagagaaag acgtcctgta
      721 gaactggaac aattcgactt cttaggaaat atttatcata atcctcaatc ggggccccat
      781 caataagaat ctctccttgg tctatatcat agagcctggt tagaaggctc ataatggttg
      841 tttttcctga accagtggct ccaacaaagg ctatggtttc tccggccttt acctggaagc
      901 ttacattttt aagaacggtt tcatcttttt cataactaaa gcaaacattt ttaaattcca
      961 catctccttt tagatgttga ggatttagct tgggatcatt gacaatggtt tcttcttctt
     1021 ccagtagcat aaaaactcgt tcaatactag ccatggcaga ttgaaatata gtgaactttt
     1081 ctgaaagatc ctggatgggt cggaagagtt tttgtacata gatggtaaag gcgatcacca
     1141 cacccacttc tatacttccc tctattgcaa atttcccccc aaatataata acaaggatta
     1201 ggctagtata gtaaagaaga ttgataaagg gtctaaaaat agcatagatt tgcagttcac
     1261 ccttgctggc tacatggtac ttttcattat cttctgcaaa attctcaatg aatttctttt
     1321 cctggttaaa aatttgtatg gttttcattc cactaagatt ttcatttagg gtggtgttga
     1381 ttttagcaag ctttacccta acatcccggt aggctagtct aaccttcctt tggaaaatta
     1441 gggttactac aacaagaagt ggcataatgg aaagagaaat aaagctaagc tttacattca
     1501 tgctaaacat cataaagata ctacctagta taatggccac atcactaagg acagttacaa
     1561 tgacattggt atagagctca ttgatattgc caaggtcatt tgtcatccgg gtaaccagtc
     1621 gtcccacagg atttttttca tgaaagctaa ggcttaatct ctcgagcttg gcaaaaaggt
     1681 ctgagcggat tctttgaatg acccgctggc cgacatagtt tagggcaatc tgttctaaca
     1741 tggcactaag ataggtaaga atagataggg ccagtaaaaa taaggtcaga ttaattattt
     1801 ttttgtatcg ataatccttg taggctctat tttcttcttg gcttaaagag cggctaaggc
     1861 cgtctttttc aaggttttga tcttttagaa caacagaagt gggctcctta ctaatggaaa
     1921 agtttccttc ttctaaagca aagccctggg gctcttcttg aagataactt tgcctgggta
     1981 caagatagtc atctattata gtagatataa tcctgggttg taaaagatca aagagggtcc
     2041 cggcaagaag caaaagggca ctcataagta aaagcaacca aaggcccttg gtgtatttag
     2101 cgagtttttt tactagcttg ccatcataaa acttctcaag tttttgttca tcaaattggt
     2161 tcatagccgg ctccttggat tttatcctcg agaagttgtt tttcatagac ctgatagtag
     2221 cgtcccctaa gggctaggag ttcttcgtga ttgcctcgct caacaatgcg tccttcatca
     2281 ataaaaataa tttcatctaa atgctgcagg gttgagatgc ggtgggaaat aataatggaa
     2341 ctatgactgg caaggtctac tcttagtttt cttaggatgg cttcttcggt ttttgtatct
     2401 actgctgaga gactatcatc taggataaga atgggcgcat ttttataaag ggctctagca
     2461 atggaggttc gctgtttttg tcctccgctt agattgactc ccttttcccc aagaaaagtt
     2521 tggtaactct gggggaaact ttcaatttct tcgtgtacat gggcatcctt ggccgcctgg
     2581 agaatcttat cttttctggg tcttgtatca ctaagggcaa tgttattttc tatttctgta
     2641 gaaaagagaa aattgtcttg gggtaccatg gcaattaaat ttctagtatc ttcaatggat
     2701 aaatcattga tatccctttt accaagaaag atctttcctc ttgggatatt gtataga
//
LOCUS       DASRNA010000109         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_185014,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000109 DASRNA010000000
VERSION     DASRNA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185014"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..999)
                     /locus_tag="VFD08_02920"
     CDS             complement(49..999)
                     /locus_tag="VFD08_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZK10001.1"
                     /translation="MKKYLCILLIISFLLAGCTKQEEYETSFFAFDTIVEFKLYEGGN
                     QEILDELKKEALRIEKLLNIHDETSVISSLKGNSLEENEISELIKLSNEMKELTQGAF
                     DPYLGRVSALWAFGTEEENIPLKEDLDKYIALKIGESLEDLDLGSIAKGYAANQLQEL
                     AKKAGVESALFNLGGNIITLGNYQEGTYNIGIQDPLDQTGEIFGFVKMGENSAVTSGA
                     YQRGFEEEGVWYHHILDPKTGYPVDNDLLSVSLVLEDSARADAYSTALFVMGFDKAKE
                     YAHLHSLPVILVRKDGAICLLNAEKFTFVLLKEEYFLLQE"
     gene            complement(1417..>2891)
                     /locus_tag="VFD08_02925"
     CDS             complement(1417..>2891)
                     /locus_tag="VFD08_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HZK10002.1"
                     /translation="YEDGIDFVPSKPAVLLGHHFSSIAGAGPINGPIQAAIFGWVPVL
                     LWIIIGSIFFGGVHDYGSLVASIRHKGKSIGEVIGINMGRQGKMLFSAFAWLTLLLVV
                     AAFTSIVASTFATVPEAASTSLMFIVLAVVFGYLVYRKGLPLGIGTVIGVALLFGCVF
                     LGRVFPLSLSKEIWIIILMVYIFVASTAPVWILLQPRDYLNSYLLYAMIIGAVLGIVI
                     YRPSLELQAFTGFNVDGQWLFPMLFVIVACGAISGFHSLVGSGTTSKQLDKEQDAKLI
                     GYGGMLIEGVLAVIALITAAYITQGELGELLQGGPVNVFSNGVGVFMTKFGIPFDLGK
                     SFVALAVSAFALTSLDTATRLNRFVFTEFFEKEGAEKQSFLTNMYVSTAVTVLLGGWL
                     AYGGFAKIWPIFGSANQLLAALALLAIAVWLKNIGKNYKMIIPPMMFMLVVTLFALVL
                     LIKTNIEAQNWILVFFPALLFILAIILAVQGYKIIFSKDE"
BASE COUNT          961 a          597 c          493 g          840 t
ORIGIN      
        1 atacaaatcc gttaaaataa aaaactatta aaaaatcagc agttttttct attcttgcaa
       61 taaaaaatat tcctccttaa gtagaacaaa agtaaatttc tctgcattta gaaggcaaat
      121 agccccgtct tttctcacta aaataactgg aagagaatgc aaatgggcat attcttttgc
      181 cttatcaaag cccattacaa aaagagctgt actataggca tctgccctag cagaatcttc
      241 taatactaaa cttacgctta aaagatcatt gtctactgga tagccagtct ttggatctaa
      301 aatatgatga taccaaaccc cttcttcttc aaagcctctt tgataggcac cgctggtgac
      361 agcggagttt tctcccatct ttacaaagcc aaatatttca cccgtttgat ccaatggatc
      421 ttgaattcct atattgtagg ttccctcttg atagttacca agagtaataa tattgccccc
      481 aagattaaat aaagcgctct ccacccccgc tttcttagcg agttcttgta attgatttgc
      541 agcgtaaccc ttggcaatac tgccaagatc taaatcttct agggactctc ctatctttag
      601 tgctatatac ttgtctaaat cttccttaag aggaatgttt tcctcttctg taccaaaggc
      661 ccaaagagca gaaactcttc ccaaataggg atcaaaggct ccctgggtta gctctttcat
      721 ttcattagac agtttgataa gctcggaaat ctcattttct tctaaggaat tgccctttag
      781 ggatgagatt acactggtct catcatggat atttaacagt ttttctattc taagggcttc
      841 tttttttaac tcatcgagta tttcttggtt gcctccctcg tatagtttaa actctactat
      901 ggtatcaaag gcaaaaaaac tagtctcata ctcttcttgc tttgtacagc ctgctaataa
      961 aaagctaata attaatagta tacataaata ttttttcatt ataactcctg agttcacttt
     1021 accatcctaa tagacccaat gcaatcaaga aagtgcaata tttttatttt acttaggcca
     1081 actttttgcc tatcataata ggcaaaaaaa taggagatcc ttggatctcc tataattttg
     1141 gattcttaag tgctaaatag tgaaagccca aaaaacccct tcgaataaga tgaatttctt
     1201 tgtttctctc ccatataagg ggatcaatat aaaagataat cccttctttt tcgagacttt
     1261 caaaggcttt gtcattatct ggtttttcca gcgaaatcac cgattcgcga agaggaactc
     1321 aggaagtaga tgcttcaaac acagaaattg tgacgtgttt tacagagctt ctctttttta
     1381 aaaaactgcg tgccggttca ttgataatta gtttcactac tcatcctttg agaatataat
     1441 cttgtaacct tgaactgcta agataatcgc taatataaag agcagggctg ggaaaaatac
     1501 aagaatccaa ttttgtgctt caatatttgt tttaattagt aaaactaaag caaatagggt
     1561 cacaacaagc ataaacatca ttgggggaat aatcatcttg tagttttttc caatattttt
     1621 tagccataca gcaattgcta atagtgcaag agctgctaaa agctggtttg ctgagccaaa
     1681 gattggccaa attttagcaa atccaccata ggctaaccat ccaccaagaa gaactgtaac
     1741 agcagtagaa acatacatat ttgttaagaa tgactgcttt tcagctcctt ctttttcaaa
     1801 aaactctgta aagacaaaac gatttaaacg tgttgccgta tcaagactgg taagagcaaa
     1861 ggctgaaaca gcaagagcaa caaaggactt accaagatca aagggaatcc caaattttgt
     1921 cataaaaacg cccacgccat tactaaatac attgacaggt ccgccttgaa gtagttcgcc
     1981 aagctcgcct tgagtaatat aggctgctgt aataagggca ataacagcta gaacaccctc
     2041 aataagcata ccgccatagc cgattagttt ggcgtcttgt tccttatcta attgctttga
     2101 agtcgtacca gatccaacaa gagagtggaa acctgatatg gctccacagg caacaataac
     2161 aaaaagcata ggaaagagcc actgaccatc gacgttaaag ccagtaaaag cttgtaattc
     2221 aaggctaggt ctgtagatta cgattcctag aacagctcct ataatcattg cataaagtaa
     2281 ataggagttt agataatctc taggttgcaa taagatccat actggtgctg ttgaggcaac
     2341 aaatatgtag accataagga taataatcca tatttccttt gaaagggaaa gtggaaatac
     2401 tcttcctagg aaaacacagc caaataaaag ggctacgcct atgactgtac caatgccaag
     2461 aggaagacct tttcgataca caagatatcc aaaaacaaca gctagaacaa taaacattag
     2521 ggatgtagaa gctgcttctg ggacagttgc aaaagtacta gcaacaatac ttgtaaaagc
     2581 tgctacaact agtagcagtg tcagccatgc aaaggcggaa aaaagcattt ttccttgtct
     2641 tcccatgttt atgccaataa cttcaccaat actttttcct ttgtgacgaa tagatgctac
     2701 tagagaacca tagtcatgaa caccaccaaa gaaaatactt ccaattataa tccatagaag
     2761 tacaggtacc catccaaaaa tcgcagcctg tataggacca ttaatggggc ctgcaccagc
     2821 aattgatgaa aagtggtggc ccaaaagcac tgcgggcttt gaaggcacaa agtcgatgcc
     2881 gtcctcatat t
//
LOCUS       DASRNA010000110         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_185588,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000110 DASRNA010000000
VERSION     DASRNA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185588"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VFD08_02930"
     CDS             <1..731
                     /locus_tag="VFD08_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZK10003.1"
                     /translation="RQKKVKIALVGKYVELKDAYISTCEALEHAGIELGLRPELVWIN
                     AEELSVSNSRELLSSVHGLILPSGFGSRGLAGMLEACRYARLHKLPFFGIGFGMQAAG
                     LEFARNVLGLSDANSTEFDPTTSNPLIDIDKEDTSMRLGSQGIDLSPGSLFEKLYGES
                     FIKERHRHRYDFSSSYQKVFEEKGLRFSGLSRDGLWVEAMEMSEHPFYLACQYHPEFK
                     SRPTRVHPLFRGFMQATRQYKENR"
     gene            817..1107
                     /locus_tag="VFD08_02935"
     CDS             817..1107
                     /locus_tag="VFD08_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZK10004.1"
                     /translation="MELTDQEARELAEFYKIFGDPTRIRLIYLLSSGELNVGELAKKV
                     NLSLSAISHQLRILKVHRIVKNRKEGTAVYYSLDDEHVYTLISQGYEHIGHR"
     gene            1058..>3101
                     /locus_tag="VFD08_02940"
     CDS             1058..>3101
                     /locus_tag="VFD08_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZK10005.1"
                     /translation="MFILSSAKGMSISAIDKMKSWHFENLTCSSCAGKIEEKVRKLDV
                     GQVNMNTLTMEIRIEKPLKNMQEILQKIVDETEPGVKVRGEASKKSRSPLLIRVGIGA
                     FLFAISQLVASPQPIQLLAYAILSYDILYKSVHNLFRGQLLDEYFLMSVATLGAIALK
                     DYPEALAVMLFYQIGEYLQERALTRSQKSIEALLELRPDQVRVRREGRWIDLAPEELL
                     IGDLIRVLPGERLAVDSVVYEGESQVDTSAITGESRPSRVGYQDPVYSGSINLDSSMD
                     LVVKKEYNDSTLARILALVQDAGFKKAPVERFITRFSKIYTPVVVGVAILLAIIPTLM
                     GLEGDWIRRALIFLVISCPCGFVISVPLSFYAGIGKASSQGILIKGGTDLETLSKLQG
                     IVFDKTGTLTKGEFTLQEVKTNYISRRELIKIAASLESRSTHPIARSIVNAHDGNIYQ
                     FDKYVEIRGKGIEGFRRQERYLIGSGNFLEEEGIKIDQDQPGLIHISRGDEYLGWLEL
                     GDKLRDDTSELMQGLKKLGLRKIMLSGDKDEITQALAKEIGIDDAYGELLPEDKVRIY
                     EELRKTLSPLAFVGDGINDAPVLARSDLGIAMGGLGSDAALEAADMVLMEDKPIKILR
                     GIEISKKTMRHLWQNIIFIFSVKLIVLALGALGLASMWMAVFADVGVTLIAILNSLR"
BASE COUNT          892 a          648 c          770 g          791 t
ORIGIN      
        1 ggcggcaaaa aaaggttaag attgccctgg tgggaaaata cgtagaatta aaagacgcct
       61 atatatcgac ctgcgaggcc ctagagcatg caggaattga gctgggccta aggccagaac
      121 ttgtctggat caatgcagag gagctaagtg tttctaatag tagggagcta ctttcttcag
      181 tccatggtct tattttaccc agtggttttg gaagcagggg gcttgctggc atgctagaag
      241 cctgccgcta tgctaggctg cataagctgc ccttttttgg tattggcttt ggcatgcagg
      301 cagcaggcct tgaatttgca agaaatgtcc taggacttag tgatgccaat tccacagaat
      361 ttgatcctac aacgagcaat cctttaattg atatagataa agaagatacc tctatgcgcc
      421 tggggtccca ggggattgac ctaagcccag gcagtttatt tgaaaagcta tatggtgaga
      481 gctttatcaa ggagcgccac cgccaccgct atgatttttc ttcttcctat caaaaagtct
      541 ttgaagaaaa aggcctgcgc ttttctggtc tctctaggga cggtctttgg gtagaagcca
      601 tggaaatgtc agagcatcct ttttatctgg cctgtcaata ccatccggaa tttaaatcca
      661 gacccaccag ggtccatccc ctctttaggg gctttatgca ggccactagg cagtataagg
      721 aaaatagata agtaaaaagc ttcttcttga ctggaaggag cttttttaat atactatata
      781 tgagcaagtg ctcaagtata gaagaaggag taatcaatgg aattaacaga ccaagaagca
      841 agagagcttg ctgaatttta taaaatcttt ggtgatccca caagaatacg ccttatttat
      901 cttctgagtt caggagaact caatgtggga gaactggcaa aaaaagtaaa tttgagtctt
      961 tcagcgatca gccaccagct gcggatccta aaggttcatc gcattgtcaa aaacagaaag
     1021 gaaggaacgg ccgtctacta tagtcttgat gatgagcatg tttatactct catcagccaa
     1081 gggtatgagc atatcggcca tagataaaat gaaaagttgg cattttgaaa atcttacctg
     1141 ctctagttgt gcaggaaaaa tagaagaaaa agtaagaaaa cttgatgtgg gccaggtaaa
     1201 tatgaatacc cttaccatgg agatccgcat tgaaaaaccc ctaaaaaaca tgcaggagat
     1261 cctgcaaaag attgtcgatg aaacagaacc aggagtgaag gtcagagggg aggccagtaa
     1321 aaaaagcagg agcccccttc taattcgggt tggcataggg gcttttcttt ttgcaatctc
     1381 ccagctggtg gccagtcccc agcccataca acttttggcc tacgctattt taagttatga
     1441 tattttatat aaatcagtcc ataacctctt tcggggccaa ctcttagatg agtattttct
     1501 tatgagtgtg gccaccctag gagccatcgc cctaaaggat tatcctgagg ccctggctgt
     1561 tatgctcttt taccaaatcg gagaatatct gcaggaaaga gcccttacaa gaagccaaaa
     1621 atccattgaa gccctactgg aacttaggcc agaccaggtc cgggtgcgaa gagagggcag
     1681 gtggattgat ctggcaccgg aagaactcct tataggagat ttaatccggg tcctacccgg
     1741 agaaagactg gcggtagact cggtggtcta tgaaggagaa agtcaggtag atacctcggc
     1801 cataactggt gagagccggc ccagcagggt aggctatcaa gacccggtct atagcggcag
     1861 catcaatcta gactcctcta tggatctggt ggtaaaaaaa gagtataatg attcaaccct
     1921 ggcaagaatt ctggccctgg tccaggatgc aggctttaaa aaagcccctg tggagcgctt
     1981 tatcaccagg ttttcaaaga tctatacccc cgtggtagta ggggttgcta tcctgctagc
     2041 tattattccc acccttatgg gactagaggg agactggatt cgcagggccc tgatcttcct
     2101 agtcatcagc tgcccctgtg gctttgttat ttctgttccc cttagcttct atgctggcat
     2161 aggcaaggcc tcttcccagg gcattcttat taagggaggc acagatcttg agaccctctc
     2221 caagctccag gggattgtct ttgataagac aggaacccta accaagggag agtttaccct
     2281 gcaagaagta aagaccaact atatctccag aagagagctg attaagattg ctgcttcttt
     2341 agaaagtagg tctacccatc ccattgccag aagcatagta aatgcccatg atggcaatat
     2401 ctatcaattt gataagtatg tggagattag gggcaaggga attgaaggct ttcgcagaca
     2461 ggagcgctac cttataggta gtggcaactt cctagaagaa gaaggcataa aaatagacca
     2521 ggaccagccg ggcctgattc atatttcaag aggagatgag tatctgggtt ggctggaact
     2581 aggagacaaa ctccgcgatg ataccagtga actaatgcag gggctaaaaa aacttggtct
     2641 aagaaagatt atgctcagtg gggataaaga cgaaatcacc caggccctgg caaaggaaat
     2701 aggcattgat gatgcctatg gtgagctcct gccagaagat aaggtccgaa tctatgaaga
     2761 gctaaggaaa actctttctc ccctggcctt tgtaggagat ggcatcaatg acgccccggt
     2821 tcttgcaaga agtgatctgg gcattgccat gggaggactt ggttcagatg ctgcccttga
     2881 ggcggccgat atggtcctga tggaagataa gcccattaag atccttcggg gcattgagat
     2941 ttctaaaaaa accatgagac acctctggca gaatattatt tttatcttta gtgtaaaatt
     3001 aattgtcctg gcgctgggag ccctaggtct agccagtatg tggatggcag tctttgctga
     3061 tgtgggggta actctgatag ccattttgaa ttccttaaga a
//
LOCUS       DASRNA010000111         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_998118,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000111 DASRNA010000000
VERSION     DASRNA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998118"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFD08_02945"
     CDS             complement(<1..614)
                     /locus_tag="VFD08_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZK10006.1"
                     /translation="MGKKIKELFFAERGQAAVLVALSLTALLGFAAISVDYGFLANER
                     RSLQNAADAAALAAAWELPNKNNVEAKGKEYAIKNASELAGGEIRFEYPNGSKEVKVD
                     LAYPYNTFFARVLGQDSAVISASATAEKKSKWKGEALPLFNIAYDYEMNPTFDAYGKV
                     GPGTYGGILNFGGEKTVDENKNISSAYFEIDYENGIVIDKGIAYG"
     gene            complement(639..1061)
                     /locus_tag="VFD08_02950"
     CDS             complement(639..1061)
                     /locus_tag="VFD08_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZK10007.1"
                     /translation="MKKLLVLKRNEKGQGLVEFALVLPLLLLLLLGIIQFGIIFSSQI
                     AITNAAREGARVAAVGANNGEIRDRINSSIGGHIFLPQLEDANIVYPASNLVEGDEVT
                     VTISGVQLKLIVPVPDVFVPGSVIILSGQASMRLEQGP"
     gene            complement(1073..1624)
                     /locus_tag="VFD08_02955"
     CDS             complement(1073..1624)
                     /locus_tag="VFD08_02955"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZK10008.1"
                     /translation="MVESINNFILFGLIVLAVYFDLSTKKIPNAITFPVILWGLVVYT
                     VFGGFVGFKFTLIGFLVGLAIFLLPFILGGMGAGDVKLMAAIGALMGWKFALKTAVAS
                     ALVGGVIVIIYSLYKKTLWTTIKSAVGLILRPLVFILAKSFNSERLEKAHKYFSEQSV
                     QYEKHYIPYGVAIGLGAIIVFIV"
     gene            complement(1888..2061)
                     /locus_tag="VFD08_02960"
     CDS             complement(1888..2061)
                     /locus_tag="VFD08_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZK10009.1"
                     /translation="MKDLMMRLITEEEGQGMVEYGLIIALVAVAVITALTAMGGGVSD
                     IFDKIKGSLGVEG"
BASE COUNT          676 a          502 c          293 g          689 t
ORIGIN      
        1 ccataagcaa tccctttatc aatcactatc ccattttcat agtctatttc aaagtaggca
       61 ctgctgatat tcttattctc atcaacagtt ttctcccctc caaaattcaa aattccacca
      121 taagtgcctg gtcccacttt gccataggca tcaaaagttg gattcatttc ataatcatag
      181 gctatattaa ataaaggtag ggcttcgccc ttccattttg attttttttc tgcagtagca
      241 ctggctgaaa tcactgcact gtcttgaccc agtactctag caaaaaaggt attataaggg
      301 taggctaggt ctacctttac ttcttttgat ccattaggat attcaaacct tatctcacca
      361 ccagctaatt cagaggcatt ctttattgcg tattcttttc cttttgcttc tacattattt
      421 ttattgggaa gctcccatgc tgccgcgagt gctgctgcat ctgctgcatt ttgtagactg
      481 cgtctttcat tggctaaaaa accataatca actgatatag cggcaaatcc aagcagtgct
      541 gttagactaa gagcgaccaa aactgctgct tggcctcttt cagcaaaaaa cagttctttt
      601 atttttttac ccatcttgat tcctcctttt ttctctcact aaggcccttg ttctaatctc
      661 attgatgctt gcccgcttag aataatcaca cttccaggca caaaaacatc tggaactgga
      721 acaatcagct taagttgaac accgctgata gttacagtga cttcatcacc ttctacaaga
      781 ttagaagcag gataaacaat attagcatct tctaactggg gcaaaaaaat atgtccacca
      841 attgaactgt ttattctatc tctaatttct ccattatttg caccaacggc ggcgactctt
      901 gccccttctc tagctgcatt tgtaatggca atttgtgagc taaaaataat cccaaactga
      961 ataattccta gcagcaaaag aaggagtagc ggtaaaacta aggcaaactc cactaaacct
     1021 tgtccttttt catttctttt tagcacaagc agttttttca caatctctca cctcacacaa
     1081 taaagactat tatcgctcct aggcctatgg ctactccata gggtatgtaa tgcttttcat
     1141 attgtactga ttgttctgaa aaatatttat gtgctttctc aagtctttca ctgttaaaac
     1201 ttttagctag tataaataca aggggtcgca aaattaaacc caccgcactt ttgattgtag
     1261 tccatagtgt ctttttatat agcgaataaa taattacaat tacaccacca actaaagcag
     1321 aagcaacagc tgtcttaaga gcaaacttcc aacccatcaa ggccccaatt gctgccatta
     1381 acttaacatc gccagcgccc attccaccta gaataaaagg taataaaaat attgctaaac
     1441 caactaaaaa accaataaga gtaaacttaa agccaacaaa tccaccaaat acagtgtata
     1501 caactaatcc ccaaagtatt acaggaaaag taattgcatt ggggatcttc tttgtgctta
     1561 aatcaaaata tacagctaag acaattaagc caaataaaat aaaattatta atactctcga
     1621 ccatgtaatc tccttaagct aatgcactta aaacgttctc ataaaaacct ttaacacctg
     1681 tcccaaacaa ttcaatagag gcaaatacca aagtagcaat caaagatagg atgagagcat
     1741 actcgaccaa gccttgtcct tcttcttgaa ataacaactt atacaaacag tccatgttta
     1801 gcccttttgt ttaatttctg gaaatctgct ttactagaag cagatttcca gaaaaatagt
     1861 tagataaacg atttacccgc ctgttcctta gccttccaca cccaaactgc ctttgatttt
     1921 atcaaaaata tcactcacac caccacccat agctgttaag gcagtaatta ctgctactgc
     1981 taccaaggca ataattagcc catactctac cattccttga ccttcttctt ctgtgataag
     2041 tctcatcatt aaatctttca ttttttcccc cttcgtttat tgttttatca ttatcttctg
     2101 gtcattcttt ttgaaataaa tatcatgata actattattt tataactatc ccttattatt
//
LOCUS       DASRNA010000112         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_35349,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000112 DASRNA010000000
VERSION     DASRNA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35349"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..990
                     /locus_tag="VFD08_02965"
     CDS             136..990
                     /locus_tag="VFD08_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK10010.1"
                     /translation="MNKEKIAILVDSGSDVPKNLIKEYGMYVAPLTIIYKDGEYKDGI
                     DILTEDVYSRLSEEIPTTSLPTGETIQAIFNQIKENGYKKVLAITISSGLSGTYNMIR
                     MIGEQEDDLDVYVLDTKNISIASGFNAIQAAEYIKEGMDWDTLTKRVSENILNSKVFF
                     YVSTLKYLQKGGRIGLVASLLGSGISLKPIISCNDDGVYFTKAKVIGESRALNKIIEL
                     AKDHIGNSEEYNIGVVHAAAPAKAETISKRLLQKLPNPKLSISGEISAALGVHTGPDS
                     IGVVVQKI"
     gene            1479..2474
                     /locus_tag="VFD08_02970"
     CDS             1479..2474
                     /locus_tag="VFD08_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZK10011.1"
                     /translation="MKELYLVTGAAGHLGSTIVRQLLKLEKSIRILVLPEEKNIPQGE
                     FEVFYGDVCDKEDLKAFFNNPQKKELIVIHCAGIVSVATKYVKKVYDVNVTGTKNVVD
                     ISLEKRVKKLVYVSSVHAIPEKAKGETITEVSQFNPEDVLGFYSKTKAEASSYVLAAI
                     KKGLNANIVHPSGIAGPFDHGKGLLTALAIDYYQGQLTSGVNGGYDFVDVRDVAGGII
                     ACCEKGRSGQSYILSNEYFTIREILFMLHEITGKKEITRFLPLWFLKLIAAPAEIYYK
                     ILKRPPLFTANSISIINSNAKFSHQKASAELGYTTRDMKETLIDTINWLKENKRL"
BASE COUNT         1000 a          436 c          574 g          819 t
ORIGIN      
        1 attaatagca ggaggctctt gtcacttgga aaagctgcat caaagattgt acctataatc
       61 tagcataata aatgaattat catcagtcgt atagggttct taacagggta tattgtatat
      121 attggaggtg ttaaaatgaa caaagaaaag attgctatac tagtagactc cggctcagat
      181 gtgcctaaaa atctaataaa agaatatgga atgtatgtag cacccctaac tatcatttat
      241 aaagatggtg agtataaaga tggcatagat attttaacag aagatgtata ttcacgtctt
      301 agtgaagaga ttccaacaac atctttacca acaggtgaaa caattcaagc aatatttaat
      361 cagataaaag aaaatgggta taaaaaggtt ttagcaatta ctatatcaag tggtttgagt
      421 ggcacataca atatgataag aatgatagga gaacaagaag atgatttaga tgtttatgtg
      481 ctggatacaa aaaacatttc aatcgcgagt ggttttaatg caattcaagc agctgaatat
      541 attaaagaag gaatggattg ggacaccctt actaaaagag tgtcagaaaa tattttaaac
      601 tctaaggtgt tcttttatgt ctcaacccta aagtaccttc aaaaaggggg cagaattgga
      661 ttggtggcct ccctactagg tagtggtata agcttgaaac caattatttc ctgcaatgat
      721 gatggagtat attttacaaa agcaaaagta attggagaaa gtagagcctt aaataaaatt
      781 atagagttag caaaagatca tataggaaac tcggaggaat acaatatagg agttgtccat
      841 gctgcggcac cagctaaagc agagactatt agcaaaagac ttctgcagaa gctacccaat
      901 ccgaaattat caataagtgg agaaataagc gcagctttag gagtgcatac tggaccggat
      961 tcaattggtg tggtggttca aaagatatag acagtaagat taagtcctac aaatatgctg
     1021 gggatttctt gagactcttt aagactagcc ctatcttaag cctagttttt aagacaataa
     1081 gaagagcaag gggacaataa aaacaagcgg ttttaacgag cccatcaata attaggctat
     1141 aaaagctatc cgcatcacat atttacccaa aaaagcaagt ccttgaattc aaggactttt
     1201 ttaccgtaga aatatcaaga tcttaggtgt ttttctaagt ctcttgaatt gatatttgta
     1261 tagagagcag tacaaatata aaagatctgt tttgaaaata aaaaatattt ttgtagttgc
     1321 aaaaacttta tggggtataa gtaagaagaa gaatacagta agaaaacacc cataagcctt
     1381 ataaatactg ctatcagaca agctgaaaga agaagctgac aagtaatttg attattagag
     1441 aagccacttt tgtcttttat tggtaagaag gtgataatgt gaaggagtta tatctagtaa
     1501 caggagcagc cggacatctt ggtagtacca tagtgcgcca attacttaag ctagaaaaga
     1561 gtataaggat tcttgtgctt ccagaggaaa aaaatattcc ccagggtgaa tttgaagtct
     1621 tttatggaga tgtatgcgat aaagaagacc ttaaagcctt ttttaataat ccacaaaaga
     1681 aagaactaat agttattcac tgcgcaggaa ttgtttcggt agccacaaaa tatgtaaaaa
     1741 aagtttatga tgtaaatgtc actggtacaa aaaatgttgt ggatataagc ttagagaaga
     1801 gagttaaaaa gcttgtctat gttagttctg ttcatgcaat tcctgaaaag gcaaagggtg
     1861 agacaattac ggaagttagt cagtttaatc cggaagatgt tttaggcttc tattctaaaa
     1921 ctaaggctga agctagcagc tatgttctgg ctgccataaa aaaaggacta aatgcaaata
     1981 ttgttcatcc atcaggcata gctggtccct ttgatcatgg gaaaggactt ctaacagccc
     2041 ttgccattga ttactatcaa ggtcaactta cctcaggtgt aaatggtggt tatgactttg
     2101 tagatgtacg tgatgtggca ggtggaatca ttgcatgttg tgagaaggga agaagtggcc
     2161 agtcctatat tctttcgaat gagtatttta ccattagaga aatccttttt atgcttcacg
     2221 aaattacagg taaaaaagag atcacaagat ttctacccct gtggttttta aaacttatag
     2281 cagcccctgc agaaatttat tataaaatac tcaaacgccc ccccttattc actgctaatt
     2341 ctattagcat cataaactct aatgctaaat tttctcatca aaaggcaagt gcggagcttg
     2401 gctataccac tagagatatg aaagagacct tgattgatac aatcaattgg cttaaagaga
     2461 acaagcgact ttgattcata cccctgactt atccttctta agggtgcggt tataaagagc
     2521 ttagatgata tgtccagaaa ccccttgatt gatgatgcat aaattaaaat gacccctact
     2581 aattggagaa aaactaagta gagggtcatt ttttgtggaa attaaggtcc tgccaaagga
     2641 agctatttgg agatagggac tgtataggca agtcccataa gaaggagtaa aggcgggcta
     2701 atcccctttt gataagggcc ctctttaatt aatacgttct ttgctatctc catcaaaaaa
     2761 gtgtaaggca tctttatcag ggctaaattt atatttttta ttttctgtgt aatccaagtg
     2821 gcctggaac
//
LOCUS       DASRNA010000113         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1078153,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000113 DASRNA010000000
VERSION     DASRNA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078153"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..2191
                     /locus_tag="VFD08_02975"
     CDS             356..2191
                     /locus_tag="VFD08_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HZK10012.1"
                     /translation="MNKIASAIKRIFKGAGKSFHRFPAAIVSALVISMVAIMEIRMDW
                     QVRQDYSLLFTSIQLSFLLGAIFSMAAVALDEIRFEKEKSKSLLANLSGILLALVAFL
                     LLYFLGGKTTIDGTYLSSIAMARISVAIFISGVAFVYLVSKAQMIDSFSDSFFINHRA
                     FIISAIYGLVIMAGLSGVLGAFEALVYPAMNYRIYSYLGVIVGFVTYTIFLGYFPSFR
                     GLEDKEEIEKIKEQPSFIYVLFEYILIPILMALTVVLLIWTLRVILSGLDVPFSQLSS
                     IASSYVIIGIWLHIMVASHKSKLSKFYKQIYPFAGILILAFEAWALIVQVNRLGFTRT
                     EYSFFMIWIFASISILLLLVLKKGAYRKMAITAVIISILWVLPVIGMEDITFSSQLNR
                     LEEHLISEGLLVGENLIAKDTEIKQEKRGQITQAVDFISYSEKTNLPIWFTEGLNEEK
                     IFKETFGFEKTYGVYEDFSEYYYANFVLDTNSIDISNYKLSINVLAYERKSDDIYFEG
                     EDGTYEMTWLHGDSGVPKLTVSYEGDIIIEEDMQEYIGSLLTKYPPQEMGGRVKSLPF
                     EEMSLVVEKEDISLLVVFSNIEAYYMEEGEPDYYLNPHGIYVKFN"
BASE COUNT          717 a          362 c          475 g          710 t
ORIGIN      
        1 gcagttacaa tgattctaaa ctttgtgggc gaattcctat ggtggaagtt tatcgtattt
       61 agaggaagtg aaaataccaa taaactagca aaaaaaagcc agacaagaaa aagtgacttg
      121 taaaagcgag tagagtaaag acttactaaa acccagaaat gggttttttc ttttttagaa
      181 aaattcctta gaggaccaaa cttgacttag gatatcttat ttattgccgg aaaagaggga
      241 ggctcctttg aaaatatgct aacttagccc atgggtgttt tttcactggg taagtaaagg
      301 agtaaacttg tgggtataat ataaagtaag ttagtaggaa ggggaaaaat taattatgaa
      361 taagatagca tcagctatta aaaggatctt taaaggggca ggaaaatctt ttcaccgttt
      421 tcccgccgcc atagtaagtg cccttgtaat aagtatggtt gcgataatgg aaataagaat
      481 ggactggcaa gtaaggcaag actacagcct cttatttact agtattcaat tgtcctttct
      541 tctaggagcc atattttcta tggcagctgt agccctggat gaaataagat ttgaaaaaga
      601 aaaaagtaaa agtctactag caaatctatc tggtatttta ctcgccctgg tggccttttt
      661 acttctatac ttcctggggg gaaaaaccac cattgatggg acctatttat ctagtatagc
      721 catggctagg atcagtgtag ctatatttat cagtggggtg gcctttgttt atcttgtttc
      781 aaaagcccag atgatagata gttttagtga ttcttttttt atcaatcaca gagcctttat
      841 tatttcagcg atttatgggc tggtgattat ggctggactt tctggagtcc taggagcctt
      901 tgaagccctg gtttatcctg ctatgaatta tagaatttat agttatctgg gtgttatagt
      961 tggatttgta acctatacca tatttttggg ctatttcccg agctttagag gcttagagga
     1021 taaggaggaa attgagaaga taaaagaaca accaagcttt atctatgttc tttttgaata
     1081 tatcctaatc cccattctta tggccctaac cgtagttctt cttatttgga ctctaagggt
     1141 gatcttaagt gggctagatg taccctttag tcaattatcc tccatagcat ctagctatgt
     1201 aattattggg atttggcttc atattatggt agcaagccat aagagcaagc tctcaaagtt
     1261 ttataagcaa atctatccct ttgcaggaat cttaatccta gcctttgaag cctgggcact
     1321 tattgttcag gtaaatagac tgggatttac aagaacagaa tactcctttt ttatgatttg
     1381 gatttttgcc agtatttcaa tccttctttt gctagtctta aagaagggag cctataggaa
     1441 gatggcaatt actgccgtaa ttatttctat cttgtgggtt cttccggtca ttggcatgga
     1501 ggatataact ttttcttccc agctaaatag actagaagag cacctaataa gtgagggtct
     1561 tctagtgggt gaaaacctta tcgctaagga tacagaaatc aagcaagaaa aaaggggtca
     1621 gattacacaa gcagtagatt ttatttccta ttcagaaaaa acaaatcttc cgatttggtt
     1681 tacagagggt ttaaatgagg aaaagatttt taaagaaacc tttggttttg aaaaaaccta
     1741 tggggtctat gaagactttt cagaatatta ttatgcaaac ttcgtccttg atacaaactc
     1801 aatagacatt agcaactaca agcttagtat aaatgtcttg gcttacgaga ggaaaagtga
     1861 tgatatctat tttgaggggg aagatgggac ctatgagatg acttggctac atggagactc
     1921 aggagttcct aaactcacag taagctatga aggagatatc attatcgaag aagatatgca
     1981 ggaatatata ggaagcctac ttactaaata tcctccccaa gaaatggggg gcagggtaaa
     2041 aagccttccc tttgaagaga tgagtctagt agtagaaaaa gaggatatat cccttctagt
     2101 ggtctttagc aatatagaag cttattatat ggaggaaggt gaacccgatt attatttaaa
     2161 cccccatgga atatatgtaa aatttaacta gaaaaagtca aaagagatgg agaaagaacc
     2221 ctccatctct tttttttagc cctcctagtt taagcgacct atgg
//
LOCUS       DASRNA010000114         9494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_785326,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000114 DASRNA010000000
VERSION     DASRNA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785326"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFD08_02980"
     CDS             complement(<1..456)
                     /locus_tag="VFD08_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10013.1"
                     /translation="MKAYTIVPVRTKEHLHDFTMLPFSLYDESSPWVPPLIGDYKKYI
                     CGVDNSLNEVGPNEKVIVRKNNTILGRLLVGINKDLNNYHNLAEAYISQFECTRDPLA
                     ARLLLDFASEWAKKQGMSRLKGPLSLPGGDDNRGFIIDNFDSATYIMNTY"
     gene            complement(487..1803)
                     /locus_tag="VFD08_02985"
     CDS             complement(487..1803)
                     /locus_tag="VFD08_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK10014.1"
                     /translation="MNFPSESIEVLEKLNFAGHKAYFVGGSLRDSLLGKAISDIDLAT
                     SAHPDEVMELFSDHTIYPIGLKHGTLTLIYKKFPLEITTFRTEGEYEKHRRPKSVRFS
                     TSIEEDVQRRDFTINALAYHPKEGLLDFVGGQEDLDKQILRTVGPPKDRFNEDALRMM
                     RGLRFLASLGFSLDKKSKDALFELKHLLKEVSPQRLKKELNLLLMGDYAEDVLIKYYE
                     ILGVFLPEILPMVDFEQQTPYHCYDVWTHTAKVVTYSKKDQAHRLAALFHDIEKPSTF
                     YLDERDIGHFPGHGIESAKTTKTILTRLGYSNKMQKNVLPMILHHNSSIYPEIESIAR
                     RIFYWGPKTFFDVLDLKRADNLAKEPAFIRSEKAYQEVEDMAKDYLANKPLLSYKDLD
                     ISSEQLMEMGYTGKKLGFVLEKLIMAVFAGLANEKEALVDYLLQNK"
     gene            1949..2419
                     /locus_tag="VFD08_02990"
     CDS             1949..2419
                     /locus_tag="VFD08_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HZK10015.1"
                     /translation="MKSYHYVKDKSPSKARQNWDQYFMNLARLAAARSTCDRAHVGAI
                     VVNKEHRIVATGYNGSVGRKTPHCSEVGHAIRDDHCIATQHAELNCISYCAKEGISMD
                     GCRIYVTHFPCLNCTKALIQSGITSIYYEEAYRVDDFAIHLLDTNEIIYKQIKG"
     gene            2548..2868
                     /locus_tag="VFD08_02995"
     CDS             2548..2868
                     /locus_tag="VFD08_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HZK10016.1"
                     /translation="MILTTTNSVEGKIITLYHGIVFGEVIVGINFIKDIGAGLRDIFG
                     GRSGGYERELIQARENALEELAERARALGADAVVGIDMDYEVLGQANGMLMVTASGTA
                     VGLE"
     gene            3055..3969
                     /locus_tag="VFD08_03000"
     CDS             3055..3969
                     /locus_tag="VFD08_03000"
                     /inference="COORDINATES: protein motif:HMM:NF017967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HZK10017.1"
                     /translation="MKRTILIVFALLLLPLQVFAVANWQEQTFVYGGGLSQEQIKQTS
                     QLLGIKEGEAVLESPIYGEDLESYIGGEPTLTKDMISSALVIKKEKGSGVNVEIKTPE
                     NISLVSKEQYEGAAVTAGIGDVTIFIASPFGVTGESALAGVYKAFELNGEDLSRGAMV
                     LAQEEIEVVTGISEDIKDEEDFDKAKLAQAMVEIKEEIIAAIEKEGSITREEIRRIIE
                     DILDRLDLSNLVSSVHIDSLVNFFVHFSETEGLDFSQMKEQLKGLGEELAPRVKELLK
                     EAEDSGLLDRIAIFFKELLEKIMSFLDE"
     gene            4007..4129
                     /locus_tag="VFD08_03005"
     CDS             4007..4129
                     /locus_tag="VFD08_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10018.1"
                     /translation="MGKTIKIRGSARSSGLAQTRKSKRYLLQELVRLAREKKRL"
     gene            complement(4126..4956)
                     /locus_tag="VFD08_03010"
     CDS             complement(4126..4956)
                     /locus_tag="VFD08_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZK10019.1"
                     /translation="MKFNLAIIQIDTQDNKEKNLEKLEELINEAAKKKAHIISTAEMV
                     SYLGKKTKDNIERIPGGESFQLLSKKAKEYNLWIHGGSIYEENPKDPLKPFNTTMVIN
                     PRGELVARYRKLHLFDVHIDKGPSVKESDSISPGEKIVTLNTDFGKWGLAICYDIRFG
                     ELFRLMALDGAEIFFTPANFTLETGKDHWESLLRTRAIENSCYLVAAAQIGKKPKFTA
                     YGRSMVIDPWGNIIAQASDSEGVLLCEIDLDLPKKIASQLYTLPNRRSDIYTLEKSGP
                     "
     gene            complement(5003..5506)
                     /locus_tag="VFD08_03015"
     CDS             complement(5003..5506)
                     /locus_tag="VFD08_03015"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HZK10020.1"
                     /translation="MVYLIIILVKTFEVSLSTLRIKLVTKGQRLSGSIVGFFEIIIWV
                     LVISTVLTDIQSDPWKILAYAIGYSLGNFMGITVEEWIGLGSTKIEVILPQNQTKEVV
                     QQIRKAGFGVTVVEGEGKDEKRNILIIILSRKSVNQVRQILEQTPAFVTITDIKPVYG
                     GYRALRK"
     gene            complement(5522..6892)
                     /locus_tag="VFD08_03020"
     CDS             complement(5522..6892)
                     /locus_tag="VFD08_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HZK10021.1"
                     /translation="MKRERFSSRLGFILICAACAIGLGNVWRFPYIVGRYGGGAFVLI
                     YLVFLVLLGLPIMTMELAVGRGSQKSAASSFDALEPAGSKWHNFRYLAIAGNYLLMMF
                     YTTICGWMFAYFYKMVTWEFKGLNPDGVANVFGEMVANPTYMIFWLVVTIVGGLFIVS
                     LGLNKGLERYTKSMMIMLLLIMIGLVLRSVTLPGAKEGLLFYLKPDLSSIREAGLWEV
                     IYAAMGQAFFTLSIGMGSIAIFGSYIGKERSLTGEAISIITLDTFVALMAGMIIFPAT
                     SAFGIEVGQGPGLIFVTLPNVFVNMSGGRFFGSFFFLFMSFAAFSTVLAVLENIVAFA
                     MDLWNWSRKKAFLFNAPLLILLSLPTILGFNVWESFAPLGPGTIILDLEDFIVSNNIL
                     PIGGLIYVLFCTRSRGWGWENFLSEANTGSGVKFPRAAFFYVKWILPLIILVVFIAGY
                     LEKFFL"
     gene            complement(7013..7672)
                     /locus_tag="VFD08_03025"
     CDS             complement(7013..7672)
                     /locus_tag="VFD08_03025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HZK10022.1"
                     /translation="MKFNLKGASESLEGIKKDLDKLGALDLRRLKKENTVIIHVDIIE
                     GFLNFGALHSKDSAGILPFVTGLNESLRDYKKIFVIDRHNKDAAEFNAYPAHCVEDSG
                     EDKIVKELVPYCENELIITKNSTNLFHAAPFQKFLKDNPQINDFVFVGLVTDICILQA
                     TLSLRSYFNEHNIDKNLHVLVPGVDTFDLKETHHHRGLMNLFSLYNMQMNGIHLYKEV
                     K"
     gene            7843..8277
                     /locus_tag="VFD08_03030"
     CDS             7843..8277
                     /locus_tag="VFD08_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZK10023.1"
                     /translation="MGRIYSISISKKKQTLKKPLEQVVVDSLGIVGDGHAGPGNRQIT
                     LMNYQDFLELQRDKKLTLRPGDMAENVIIEGLDFSLCSPGKRIGLGPEVIVEIRQIGK
                     EDHPSVVSKKFGASILPDKGLFCKVIQTGIMKVGDQVEIIKG"
     gene            8258..9475
                     /locus_tag="VFD08_03035"
     CDS             8258..9475
                     /locus_tag="VFD08_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HZK10024.1"
                     /translation="MRLLKVKSLDEGRKLLVELMESLNFKKEKEVSLEEALGYILAED
                     LLAQEDIPAFNRSTVDGFALKSSTTMGAGDGLPTMLKIVEDVAMGQVPKKSIKPGQAS
                     KVMTGGMLPRGADAMIMVEETETMGGDLLAVYKPARAYDHVVEIGDDCKKGDIVFSKG
                     KKITPKVIASCASLGYANLKVFVPLTVRIVSTGDELVAPGEKVAPGMTRDVNSYALEG
                     LCKNLGFEVLGKTHARDEKEEIEKTLRQEADIFLISGSSSQGDKDYLAEIVSKFTPGL
                     IFHGLSLKPGKPTILASNGKSVFLGLPGHPVSSYIVFKALLEESLRDFYGLEEALYTQ
                     AYLAHNMATTPGRTTYQPVRLEREEEGLWAHPIFGASGNLSVLAGADGYFILEDKEEG
                     AVRKDLVKVYFLD"
BASE COUNT         2994 a         1786 c         2026 g         2688 t
ORIGIN      
        1 gtaggtgttc attatataag tagctgaatc gaaattatca ataataaagc cgcgattgtc
       61 atctcctcct ggtagagaca aaggaccttt taggcggctc ataccttgtt tttttgccca
      121 ctcagaggcg aaatcaagaa gtagcctggc tgccagggga tctctagtgc actcaaattg
      181 agaaatatag gcttcggcta aattatgata attatttaag tccttattaa tacctacaag
      241 tagccttccc aagatggtgt tgttttttct aacaataact ttttcgtttg gccccacttc
      301 gtttagggag ttatctacac cacaaatata ttttttataa tctccaataa gggggggtac
      361 ccagggagaa gactcatcat ataaagaaaa aggaagcatg gtaaaatcat gaagatgctc
      421 ttttgtccta acaggaacga tagtataagc tttcataaga cctctattct tagattaagt
      481 ctaatattac ttgttttgga gaagatagtc aactagggct tctttttcat tggccagtcc
      541 tgcaaagaca gccataataa gtttttctaa cacaaaacct agtttttttc ctgtgtaacc
      601 catctccatc agttgttcag agctaatatc aagatccttg taacttagta gtggtttatt
      661 agcaaggtag tctttggcca tatcctcaac ttcttgatag gctttttcgc ttcgaataaa
      721 agccggttcc ttggctaaat tatctgctct ttttaaatct aacacatcaa aaaaagtttt
      781 aggtccccag taaaagattc ttctggcgat ggattcgatt tctgggtaaa tagaggagtt
      841 gtggtgcaag atcattggga gaacattttt ttgcattttg ttggagtaac ccaggcgggt
      901 aagaatggtc tttgtggttt tggcactttc aatcccatga cctggaaaat gtcctatatc
      961 cctttcatcc aagtaaaagg tagagggctt ttcaatatca tgaaagagtg ctgccagccg
     1021 atgtgcctgg tcttttttgc tataggtcac caccttggct gtatgagtcc atacatcata
     1081 gcagtgatag ggtgtttgtt gctcaaagtc caccatgggt aaaatttctg gcaaaaacac
     1141 tcccagaatc tcatagtatt taataagaac atcttctgca tagtctccca taagaagaag
     1201 gttaagttct tttttaagtc gctgggggct tacttccttt aaaagatgct ttagttcaaa
     1261 taaagcgtcc tttgattttt tatccaggga aaaacctagg ctggccaaaa acctcagtcc
     1321 ccgcatcatc cgcagggcat cttcattaaa gcggtctttt ggagggccta cagttcgaag
     1381 aatctgctta tctagatcct cctgtcctcc cacaaagtcg agaaggcctt ctttgggatg
     1441 ataggctagg gcgttgatgg taaaatctcg tcgctggaca tcctcctcga tactagtaga
     1501 aaaacgcacg cttttgggac gcctatgctt ttcatattca ccctcagttc gaaaggtggt
     1561 tatctcaagg gggaattttt tatagataag ggtaagcgta ccatgcttaa gacctatggg
     1621 atagatggtg tggtcgctaa agagctccat cacctcatcg ggatgggcag aagtggccaa
     1681 atctatatca ctgatggctt taccaagaag actgtctctt agtgagcccc caacaaaata
     1741 cgccttatgg ccagcaaaat ttagtttttc taagacttca atactttcag aaggaaaatt
     1801 catactcacc tccctactag tataacaaag cctaagaaga taatgaagaa ggaaaaaaac
     1861 tagcgaaata agaaaatcat gatagaatac attaagcagc tatataatgc ttttttatta
     1921 ccttatcaag ccagctttag gaggaattgt gaaaagctat cattatgtga aagataaaag
     1981 cccatcgaag gctagacaaa attgggacca gtattttatg aatttagctc gcctggcagc
     2041 agcacgtagc acctgtgata gggctcatgt aggtgctata gttgtcaata aagaacatag
     2101 aattgttgca acagggtata atggaagcgt aggcagaaag accccccatt gctcagaggt
     2161 tggtcatgcc attcgagatg atcattgcat tgccacgcaa catgcggaac tcaactgcat
     2221 tagctactgt gcaaaagaag ggatctctat ggatggttgt aggatttatg ttacgcactt
     2281 tccctgccta aattgcacca aggccctgat tcaatcagga attaccagta tttattatga
     2341 agaagcctat cgggtggatg attttgccat ccatttacta gataccaatg aaattatcta
     2401 caaacaaatc aaggggtaaa accttttact atttgcaagg gggaaaacat ccttttttgg
     2461 atgaaagagt aagtaagacc aattaagggt ataaaagata agcagcttaa aacctgtagg
     2521 ataagtctaa gagcaaggag gaaatctatg atcttaacaa caaccaatag tgttgaggga
     2581 aaaataatta ccctttatca tggcatagtt tttggagagg ttattgttgg aatcaacttt
     2641 atcaaagaca taggcgcagg tttaagagat atattcgggg gccggagtgg gggctatgaa
     2701 agagaactga ttcaagcccg agaaaatgcc ttggaagagc tagcagaaag agcaagggca
     2761 ctaggtgctg atgctgttgt tggaatagat atggattatg aggttctagg ccaggctaat
     2821 ggtatgctaa tggtaacagc atctggaaca gctgtgggct tggaataaaa aaatgtataa
     2881 ctcaccttat cagaaaaagc aaaggaacta gggcaaaaag tcttagccat agcatggtgc
     2941 tagggatttg atttctttgt ttgctagaca agagcttctg attcttggtg ttttaaatta
     3001 aaagaaataa aaagagttaa gtagtattta actatttacc tataggaggt aatgatgaaa
     3061 agaacaatct tgatagtatt tgcacttttg cttttgcccc ttcaagtatt tgctgtagca
     3121 aattggcaag agcaaacctt tgtttatggt ggtggcctta gccaggagca aataaagcaa
     3181 acatctcaac ttcttggaat taaagaagga gaggctgtat tggaaagtcc aatttatgga
     3241 gaggacttag aaagttatat aggcggagag cctaccctaa caaaagatat gatttcttca
     3301 gcccttgtta taaaaaaaga aaagggtagt ggggttaatg tagaaatcaa aacaccagaa
     3361 aacataagtc tagtaagcaa ggagcagtac gaaggagcgg ctgttacagc cggcataggt
     3421 gatgtaacaa tctttattgc ctcccccttt ggcgtaaccg gggaaagtgc actagcaggt
     3481 gtctataagg cctttgagct taatggagaa gaccttagtc gcggagccat ggtgcttgcc
     3541 caggaagaaa tcgaagttgt tacgggaata agtgaagaca ttaaagacga agaagacttt
     3601 gataaggcaa aacttgctca agccatggta gaaataaaag aagagatcat tgctgctatt
     3661 gaaaaagaag gcagtattac cagagaagaa attcgaagga ttattgaaga tattttagat
     3721 cgacttgatt tatccaatct tgtttcttct gttcacattg atagcctggt taactttttt
     3781 gtccacttct cagaaacaga agggcttgat ttttctcaaa tgaaggaaca attaaaggga
     3841 ctaggagaag aactagcacc cagggtcaag gaactattaa aagaagcaga agattcaggt
     3901 ctcttggacc gaatcgcaat attttttaaa gagcttctag aaaagataat gtcattttta
     3961 gacgaataaa aaagtttacc ttaaatagac aaagggaagt ctcctaatgg gaaagaccat
     4021 caaaataaga ggaagtgcta gaagcagtgg tctagcccaa actagaaaat caaaaagata
     4081 tcttttgcaa gaacttgttc gtttagcaag agaaaaaaag cggctttaag ggccgctttt
     4141 ttcgagggta tatatatcac tacgccgatt aggcaaggta tagagttgac ttgctatttt
     4201 tttaggaaga tcaagatcaa tttcacaaag aagaacgccc tcactatctg aggcctgggc
     4261 aatgatattt ccccaaggat caataaccat acttctgcca taggcagtga atttgggttt
     4321 ttttcctatt tgggctgcag cgacaagata gcaactattt tcaatggctc ttgtacgtag
     4381 cagggactcc cagtggtctt ttcctgtctc tagggtaaaa ttagccgggg tgaaaaatat
     4441 ctctgctcca tccagggcca taaggcgaaa gagctcccca aaacgtatat cataacaaat
     4501 agcaagcccc cattttccaa aatcggtatt gagggtaact attttttccc ctggacttat
     4561 tgaatcagat tcctttaccg aaggcccctt gtctatatga acatcaaaca aatgcagttt
     4621 tctatagcgg gctacaagtt ctcccctggg gttgataacc atagtagtat taaagggttt
     4681 taaagggtcc ttggggtttt cttcataaat gctcccacca tggatccaca gattgtactc
     4741 cttggccttt ttactcaaaa gctggaagct ttctcccccg gggatccttt cgatattatc
     4801 tttagtcttt ttaccaagat aagaaaccat ttctgccgta gaaataatgt gggctttttt
     4861 cttagccgct tcatttatta attcttctag cttttctagg tttttttctt tattatcttg
     4921 ggtgtcaatc tgaatgatag caagattaaa tttcaaaagc ttttcctttc ctgctaaaaa
     4981 gagaaggacc tgccttttct ttttattttc ttagtgctct atagcctcca tatactggtt
     5041 taatatctgt aatagtgaca aaggcagggg tttgttcaag aatttgccta acttggttta
     5101 ctgattttcg agataggata ataattaaaa tgtttcgttt ctcatcctta ccctctcctt
     5161 caacaacagt cacaccaaag cctgctttac gaatttgttg tacaacttcc ttggtctggt
     5221 tttggggtag gataacttcg atctttgtgc ttcctagccc tatccactct tctactgtaa
     5281 tacccataaa attccctaaa gaataaccaa tggcataggc caagatcttc caagggtcac
     5341 tttgtatatc agtgagcacg gtggatataa caagaaccca gatgatgatt tcgaagaagc
     5401 ctacaatcga accagaaagt cgttgaccct tggtaacgag tttaattcga agggttgata
     5461 gtgagacttc aaaggttttg acaagtatga taatcagata aaccatacac aatactccat
     5521 tctataaaaa gaatttttcc aggtaaccgg caataaagac cacgagaata ataaggggaa
     5581 gaatccactt aacatagaag aaggcagctc ttggaaactt aacaccgctt ccagtatttg
     5641 cttcactaag gaagttttcc cagccccatc ctcgacttct tgtgcaaaag agcacataga
     5701 taaggccacc gatgggtaga atgttattac ttacaataaa atcctcaagg tctagtatta
     5761 tggtacccgg tccaagggga gcaaaactct cccatacatt aaatccaagt atagtaggaa
     5821 gagacaagag gataagtagg ggggcattaa aaagaaaagc cttttttcta ctccagttcc
     5881 aaagatccat tgcaaaagcc acgatatttt caagtacagc taagaccgta ctaaaggcag
     5941 caaaactcat aaaaagaaag aagaaacttc caaaaaagcg gcccccgctc atattaacaa
     6001 atacattggg aagagttaca aaaattagac caggtccctg gcctacttct atcccaaagg
     6061 ctgaggtagc gggaaaaata atcatccctg ccataagggc aacaaaggta tccagtgtaa
     6121 taattgaaat tgcttctcct gtgaggcttc tttctttacc gatataacta ccaaatatgg
     6181 caatgctgcc cattcctatg cttaaggtaa agaaggcttg gcccatagcg gcataaataa
     6241 cttcccaaag gccggcttct ctaatgctag atagatcagg tttaagataa aagagcaggc
     6301 cttctttggc accaggaagg gtcactgagc gcaggacaag accaatcata ataagaagaa
     6361 gcataatcat catagatttg gtgtagcgct caaggccttt atttagacca agagaaacaa
     6421 taaataaacc accgacaata gtgacaacca accagaaaat catatatgta ggatttgcta
     6481 ccatctctcc aaaaacattg gcgacgccat ctggattgag gcccttaaat tcccaggtaa
     6541 ccattttgta aaaataagca aacatccacc cgcagatggt ggtataaaac atcattagta
     6601 gatagttacc agcaatggct agataacgga agttatgcca cttgcttcct gctggctcta
     6661 gagcatcaaa ggatgaagcg gcactttttt gcgatcctct tcctacggcc agctccatag
     6721 tcataatagg aagaccaaga agcactaaaa agacaagata tattaggaca aaggcgcccc
     6781 caccatagcg cccaacaata tagggaaagc gccacacatt gcctagtcca atcgcgcaag
     6841 ctgcacaaat aagtataaat cctagtctgg atgaaaaacg ttctcttttc ataataacct
     6901 ccttgaataa taatcctcat tatcccttaa gttcctgtat aaatcaaggg gaatattcgt
     6961 tattttatgc gaatctttct gggtttgtat tattttaagc ggaggtggat tgttatttga
     7021 cttctttata gagatggatg ccattcattt gcatattgta gaggctaaaa agattcataa
     7081 ggcccctatg gtgatgggtt tcttttaaat caaaggtatc gactcctggg acaaggacat
     7141 ggagattctt atctatattg tgttcgttaa agtaactacg caggcttaat gtcgcttgaa
     7201 gaatacagat atctgttaca agccctacaa agacaaaatc atttatctga ggattgtcct
     7261 taagaaactt ttgaaagggg gctgcgtgga aaagattggt actgtttttt gtgataatga
     7321 gttcattttc acaataaggt actaactctt ttactatctt gtcttcgccg ctatcttcta
     7381 cacaatgggc aggataagca ttaaattcag cggcgtcttt attgtgccta tcaataacaa
     7441 atattttttt atagtccctt aagctttcat tgagaccagt aacaaagggt aaaatccctg
     7501 cggagtcctt tgagtgaaga gcaccaaagt ttaaaaatcc ttctataatg tccacatgga
     7561 taataacagt gttttccttt tttagcctcc ttaaatctaa agctcccagc ttatcaaggt
     7621 ctttttttat gccttctaaa gactcgcttg ctcctttaag attaaatttc atatcttacc
     7681 tccttaatta atcttattgt agcagttaag aggtcaaatg ggtagtttct atttatgtca
     7741 aactggcttg ttaccatgct tttgtggacg ctgatagatt atcatggtac tataaaaaca
     7801 catagaaata gggaaaaatg ttctctatta gggggagggc ccatgggcag aatttattcg
     7861 atttccataa gtaaaaaaaa gcagaccctt aaaaaacctc ttgagcaggt agtggtagat
     7921 agcttgggga ttgtgggcga tggacatgcg ggtcctggca accgacaaat aacactaatg
     7981 aactaccagg attttttaga gctgcaaagg gataaaaaac taactttaag accaggagat
     8041 atggcggaaa atgtaatcat agaggggcta gatttttccc tatgtagccc cggaaagagg
     8101 attggcctgg ggccagaagt tattgttgaa ataaggcaga taggcaagga agaccatccc
     8161 agtgtagtga gtaaaaaatt tggtgccagc atccttccag ataagggact attttgcaag
     8221 gtgattcaaa caggcataat gaaagtggga gatcaagttg agattattaa aggttaaatc
     8281 ccttgatgag ggaagaaagc tcttagtgga gctaatggaa agtttaaatt tcaaaaaaga
     8341 aaaagaggta tctttagagg aagccttggg atatatccta gccgaggacc ttcttgcaca
     8401 agaagatatc ccggccttca atagaagtac tgttgatggc tttgctctaa aaagcagcac
     8461 cactatgggc gctggtgatg gacttcccac catgttaaaa attgtggaag atgtggctat
     8521 gggtcaagta cctaaaaaaa gcattaagcc aggacaagcc tccaaggtga tgacaggtgg
     8581 catgctacct aggggagccg acgccatgat tatggtagag gaaaccgaga ctatgggagg
     8641 ggaccttctg gcggtatata aacctgccag agcttatgat catgtggtag aaataggcga
     8701 tgactgcaag aagggggata ttgttttttc taaaggaaaa aaaatcactc ccaaggtaat
     8761 tgcttcttgt gccagccttg gttatgccaa ccttaaggtt tttgttcctc ttaccgttcg
     8821 tatagtttca acaggagatg aactagttgc tccgggagaa aaagtagctc ctgggatgac
     8881 aagggacgtc aatagttacg ctctagaggg cctgtgtaaa aacctagggt ttgaggtttt
     8941 aggaaaaacc cacgcgaggg atgagaaaga agaaatagaa aaaaccttaa ggcaagaagc
     9001 agatattttc cttattagcg gcagtagttc ccagggggat aaggactatc ttgcggaaat
     9061 tgtaagtaaa tttactccgg gacttatttt ccacggccta agtcttaagc caggcaaacc
     9121 cactatttta gctagcaatg gaaagagtgt ctttttaggc ctgccgggac atccagtgag
     9181 ttcttatatt gtttttaaag cactattaga agaaagcctg agagattttt atggtctaga
     9241 ggaggctctt tatacccagg cctacctggc ccacaacatg gctaccaccc caggaaggac
     9301 aacctatcaa ccagtaagac ttgagcgaga agaggaaggc ttatgggccc atcctatctt
     9361 cggagcatca ggaaatctat cagttttagc tggagccgat ggctatttta ttttagaaga
     9421 caaagaggaa ggagcggtaa gaaaagacct agttaaggtc tatttcttag attaaaatga
     9481 gaaatgttta ttta
//
LOCUS       DASRNA010000115         5705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_785809,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000115 DASRNA010000000
VERSION     DASRNA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785809"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFD08_03040"
     CDS             complement(<1..891)
                     /locus_tag="VFD08_03040"
                     /inference="COORDINATES: protein motif:HMM:NF023853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HZK10025.1"
                     /translation="MLKQFGNLQFNRQEMKKRLPSPIYKKWKTALYRETVMDRETADA
                     MAHAMKEWALEKDATHYTHWFIPLTGLSAEKHNTFITKEEELPLLHFSGSSLIKGETD
                     GSSFPTGGLRATFEARGFTYWDISSFAFVRGHVLYIPSIFVSYRGDQLDLKSPLLRAV
                     DTFNQRATELLHLLGGKEIKGVKPMIGLEQEYFLVRKEHYLQRPDLFYTGRTLIGAPP
                     PKGQEFEDHYLGSIPTKVSEFMQDVNLDLWRLGIFAQCEHNESAPAQFEIVSHQEIAS
                     LSIDQNMILMDVLKQKAAEHG"
     gene            complement(984..1808)
                     /locus_tag="VFD08_03045"
     CDS             complement(984..1808)
                     /locus_tag="VFD08_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZK10026.1"
                     /translation="MILARFFDGKSCRLGEIIKEEILPIGAMDLLDYLEAEPPIPQGE
                     KIPLNQVELLLPLEEVGRVFCLGKNYLDHIRELSELDGVDDTTPGAPIFFMKASKEIS
                     QDGASLTSWQDHTKSLDYEVELVLVIGKKCKNLSKEQVEEVIFGYLIGNDFTARDLQT
                     SHQQWLKGKSLDGFTALSSKVISRDELAFPPKLELRLFVNDELRQRGSTDDLLFDIPT
                     LVSELSQGLTLYPGDLIFTGTPSGVGAGMKPPTYLKSGDRVRCEIEHLGSLTTFIK"
     gene            complement(1805..2665)
                     /locus_tag="VFD08_03050"
     CDS             complement(1805..2665)
                     /locus_tag="VFD08_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4438 domain-containing protein"
                     /protein_id="HZK10027.1"
                     /translation="MKTNQNQLVEFCLQAPIHAPTMIRTYQGTPEGKNVLLPSVGGIV
                     YNFQIGDPCMDLMGDHVEPGVSLKIAEKTENDALMLFACVGNPVEVISGDAKGAKGFV
                     TGMHGGIDHTICYFKKEDLNKMALEDKIRIRALGQGLELTDYPQAHCMNLAPSLLEKL
                     GLEEKDGKLYVPVKAVIPAHLMGSGIGASSAYTGDYDIMTGDKKALEDLGLNDLCFGD
                     LVLLEDCDTRYGRQYLKGSATLGVIVHSNCVQTGHGPGVTSLITSKEELFVPMIDKKA
                     NLANYLGITK"
     gene            complement(2677..3210)
                     /locus_tag="VFD08_03055"
     CDS             complement(2677..3210)
                     /locus_tag="VFD08_03055"
                     /inference="COORDINATES: protein motif:HMM:NF021439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HZK10028.1"
                     /translation="MIREDLIIKKALDQAGLSMLARAKTAPKTKGVDHLEYILLEKED
                     FPALVEEMEKLGREHDVPFFIRDAKNLEKAQMLLLIGAFDEARNIPCCGLCGYEDCTE
                     KKKEGGRCVFPLLDLGVALGSAVSLGQTLGVDSRIMMSAGKAALNLDYFENPATIAIA
                     IPLSFTGKSIFFDRATS"
     gene            complement(3220..3723)
                     /locus_tag="VFD08_03060"
     CDS             complement(3220..3723)
                     /locus_tag="VFD08_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZK10029.1"
                     /translation="MYLHIYTGEGKGKTTAALGLGLRALGAGKKVHMIQFIKGIPYSE
                     CKVLDGLEGFSYQILGRDCFIDKKPDKKDRLLAQEALAKARAYLLEQKYDLLILDEIF
                     MALYFGLIEEAEVIPVLEERKTTEVICTGRYAPLKIMQMADLVTEMREIKHYYQKGLE
                     ARVGIEF"
     gene            complement(3724..4695)
                     /locus_tag="VFD08_03065"
     CDS             complement(3724..4695)
                     /locus_tag="VFD08_03065"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK10030.1"
                     /translation="MNLRELRRRLRADYEDKQNKESLRQDRAARLLYLNHPELKQMEN
                     NYNKMNLELLRGLMDQPGGQEEILPLLEELEKNFKQEKDNFLKEKGYKNDPREPRYSC
                     ALCRDTGTTDKGMCLCMKQSLAREIFRSEYERGPSQPSLSQMDFSIYSHEKKKEKHSQ
                     RDNILSIVESLQKFVKNFKREEGLNILFAGDVSQGKTFLAAALAVELIEEGHLVIYQT
                     APALIDKLRDLSWRYNEDNADLKSMIYDCDMLILDDLGKETRTDFSKKELFHLINLRY
                     SAGKSTLISTNLKLKDIRKDYGDAFFTRLMQNCYSPEFYGPDLRIGG"
     gene            complement(4692..5570)
                     /locus_tag="VFD08_03070"
     CDS             complement(4692..5570)
                     /locus_tag="VFD08_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HZK10031.1"
                     /translation="MNIELKPTSFDFGVTSIEHLFIDEFMPYCGLIELKVYLLGLRFA
                     QEKKEANLASLAKLLEIEYRQVAEAYRYWQKQGLVTLVHEKGDSFDVVYHSLRDIYLQ
                     SNFENKTTATSLDYSLWHQELFNEISSQLALPLSEMECQSLGEFLQGKEIDRTLVLKA
                     YEEAKKKKYRNREAMKLLSYWAEHKIKTPEEVDKLKERANFRSYQYKEILKALGQPYR
                     SPNLGEKQVIDSWLDEYKFSLETIVSEIARITRSKQNPNMNYLHAIFKRLYTEKDGTP
                     THEHEEKDLRKLGRKL"
BASE COUNT         1326 a         1277 c         1335 g         1767 t
ORIGIN      
        1 tccatgttct gcggccttct gtttaaggac gtccataagg atcatgtttt gatcgatgga
       61 taggctggcg atttcctggt ggcttacgat ttcaaactgg gccggtgctg attcattgtg
      121 ttcacactgg gcaaagatgc ccaggcgcca caaatcaaga ttcacatcct gcataaactc
      181 gctaaccttt gtggggatac tgcctaggta gtgatcctca aactcttggc ccttgggcgg
      241 gggagctccg ataagggttc ttcccgtgta gaaaagatca ggtctttgca gatagtgctc
      301 ttttctcacc aaaaaatact cctgctcaag gccaatcatg ggcttgaccc ccttgatttc
      361 ctttcccccc agcaggtgaa ggagttctgt agccctttga ttaaaggtat ctactgcccg
      421 aagaagaggg ctttttagat ccagctgatc tccccggtag gagacaaaaa tgctggggat
      481 ataaaggacg tggcccctta caaaggcgaa ggagctgata tcccagtagg tgaagccccg
      541 ggcttcaaag gtagccctaa ggcctcctgt gggaaagcta ctgccatcgg tttctccctt
      601 aataagagag ctaccggaaa agtgaagtag gggtagctct tcctcctttg tgataaaggt
      661 gttgtgtttt tctgcgctga gtcccgtcag gggaataaac caatgggtat agtgggtggc
      721 atccttttcc agagcccact ccttcatggc atgggccatg gcatcggcgg tttccctgtc
      781 cataacggtt tccctgtaga gggctgtctt ccatttttta taaatagggc ttggcagcct
      841 ttttttcatt tcctgacgat tgaactgtag atttccaaat tgttttaaca tggcaacctc
      901 cctttttaag agtctaccag taaataggga taataaaaag cccctaataa aaaatagggg
      961 ccctttggct caaataccca ggcttatttg ataaaggttg ttagacttcc caggtgctcg
     1021 atctcacagc ggacccggtc tccacttttt aagtaagtgg gaggcttcat gccggcccca
     1081 acccctgaag gggttccggt aaagataaga tctcccgggt aaagggtcag gccctgggaa
     1141 agttcgctaa caagggtggg tatatcaaag agcagatcat ccgtacttcc cctttggcga
     1201 agctcatcat tgacaaaaag cctaagctct agttttgggg gaaaggccag ctcatcacgg
     1261 ctgattacct tagaagaaag ggcggtaaag ccatcaaggc tttttccctt tagccactgc
     1321 tgatgacttg tttgtaggtc tctggctgta aagtcattgc caattaggta gccaaagatg
     1381 acctcttcta cctgctcttt tgataggttt ttacattttt tcccaatgac caggacaagt
     1441 tccacttcat aatcaagact tttagtgtgg tcctgccagc tagtaagaga agctccatct
     1501 tggctaattt ctttactggc cttcataaaa aagatgggag ccccaggagt ggtatcatct
     1561 actccatcaa gctcggaaag ctctcttata tggtctaggt agtttttacc caggcaaaaa
     1621 acccggccta cttcttctag gggtaaaaga agctccacct gatttagggg tattttttct
     1681 ccctggggga tagggggttc tgcttcaaga taatcaagca ggtccatggc ccctatagga
     1741 aggatctctt cttttattat ttctcccagg cggcaacttt tcccatcgaa aaatctggcc
     1801 aagatcattt tgtgataccc agataattgg caagatttgc ctttttatct atcataggaa
     1861 caaagagttc ctctttagag gtaattaggc tggtgactcc aggtccatgg cctgtttgta
     1921 cgcagttgga gtggacgata actcctaggg tggcactgcc ctttaggtac tggcggccat
     1981 acctagtatc acaatcttca agaagaacca ggtctccaaa acaaaggtca tttaggccca
     2041 ggtcttctag ggcttttttg tctcctgtca taatatcgta gtctcctgta taggcgctgg
     2101 aggctccaat gccacttccc ataagatggg ctggaataac agccttgaca gggacataga
     2161 gtttgccgtc tttttcctct aggcccagtt tttcaagtag gcttggagca aggttcatac
     2221 aatgagcctg gggataatcg gtaagttcta ggccctggcc cagggcccga atgcggattt
     2281 tgtcttctag ggccatcttg tttaggtctt cttttttaaa gtaacagatg gtgtggtcaa
     2341 tgccaccatg catgccggtg acaaagccct tggccccctt ggcgtcgccg gaaatgactt
     2401 ctacgggatt gccaacacag gcaaagagca taagggcgtc gttttctgtt ttttctgcta
     2461 tttttaggct gactcctggt tctacatggt ctcccataag atccatacag gggtctccga
     2521 tttgaaagtt atatactatg cctcctacag agggtagcag gacatttttt ccctcgggtg
     2581 tgccttgata ggtcctgatc atagtgggag catggatggg ggcttgcaga caaaattcta
     2641 ccagttgatt ttgattggtt ttcattataa tcctttctag ctagtggccc ggtcaaagaa
     2701 aatactttta ccggtaaaac ttaggggaat ggcgatggct atagtggcag gattttcaaa
     2761 gtaatccaga tttaaggcag cctttcctgc actcatcata atgcggctat ccacgcccag
     2821 ggtttggcct agggaaacag cgcttcctag ggctacacca agatctagca ggggaaagac
     2881 gcagcgtccg ccttcttttt tcttttctgt acaatcctcg tagccacata gaccgcagca
     2941 gggaatgttt ctagcttcat caaaggcgcc aataagcagc aacatttggg ccttttctag
     3001 gttttttgca tcgcgaataa aaaagggtac atcatgctcg cggcctagtt tttccatttc
     3061 ttccaccagg gccgggaagt cttctttttc aagaagtatg tattctaaat ggtctacccc
     3121 cttggtcttt ggggctgttt ttgccctagc cagcatggat aggccagctt ggtccagagc
     3181 ttttttaata ataaggtctt ctcgtatcat tttttcctcc taaaattcaa taccaactcg
     3241 ggcctctagg cccttttgat agtagtgttt tatttctcgc atttcggtta caagatcggc
     3301 catctgcatt atttttaggg gggcgtagcg ccccgtgcaa atgacttcag tggtttttct
     3361 ttcttctagg acagggatta cttctgcctc ttctattagg ccaaagtaga gggccataaa
     3421 gatttcatct agaattaaga ggtcatactt ctgctccaag aggtaggctc ttgcttttgc
     3481 cagggcctcc tgggctagta gcctgtcttt tttgtcgggt tttttatcaa taaaacaatc
     3541 cctgcctaga atctggtagc taaagccctc aaggccatct aggaccttgc actcgctata
     3601 ggggattccc ttaataaact ggatcatatg aacttttttt cctgctccta gggcccttag
     3661 gcccaggccc agggcagcgg ttgtttttcc cttgccttct cctgtataga tatgaaggta
     3721 catttatcct cctatacgca ggtctggtcc gtagaattct ggactatagc agttttgcat
     3781 taggcgggtg aaaaaggcgt ccccatagtc ttttcttata tccttgagct taagattggt
     3841 gctaataagg gtgctttttc ccgcactata gcgtaggttg attaaatgaa agagttcttt
     3901 ttttgaaaag tccgttcttg tttccttgcc taggtcatct aggatcagca tatcgcagtc
     3961 atagatcatg gacttaagat cggcattgtc ttcattgtag cgccagctta ggtctcttag
     4021 tttgtctatt agggcagggg cggtctggta gattaccaga tggccctctt ctattagctc
     4081 cactgccagg gcggcagcta gaaaggtttt tccctggcta acatcccccg caaagaggat
     4141 gttaaggccc tcttctcttt taaagttctt tacaaatttt tgtagggact caacaataga
     4201 gaggatgttg tcacgctggg agtgtttttc ctttttcttt tcatggctat agatggaaaa
     4261 gtccatttga cttaaagagg gctggctggg gcctctttcg tattctgagc gaaagatttc
     4321 cctggccaga ctttgcttca tacaaaggca catgccctta tcagtggtgc ctgtatccct
     4381 acaaagcgcg cagctgtatc tgggctctct ggggtcattt ttatagccct tttcttttaa
     4441 aaagttatct ttttcctgct taaagttttt ttctagttct tctagcaggg ggaggatttc
     4501 ctcctgccct cctggctggt ccataaggcc ccttagaagt tctaggttca ttttattata
     4561 attgttttcc atttgtttta gctcaggatg gttcagataa agtaggcggg ctgcgcggtc
     4621 ttggcgcagg ctctctttat tttgtttatc ttcatagtcg gcccttagcc ggcggcgaag
     4681 ttcgcgaagg ttcatagttt tctccctagc ttacgcaggt ctttttcctc atgttcatgg
     4741 gtaggggttc catctttttc tgtataaagg cgcttaaaga tggcgtgaag ataattcata
     4801 ttgggatttt gtttggagcg ggtaattcta gcaatctctg atacaatggt ttctaggctg
     4861 aacttatatt catcaagcca gctatcgatc acctgctttt ctcctagatt aggagaacga
     4921 tagggttggc ccagggcctt gaggatttcc ttatactggt agcttctaaa attggccctt
     4981 tcttttagct tatctacttc ttctggggtt tttatcttat gttctgccca gtagctaaga
     5041 agtttcatgg cttcccggtt gcggtatttt ttctttttgg cttcctcgta ggccttaaga
     5101 accagggtcc ggtctatttc cttgccctgg agaaactctc ccaggctttg gcactccatc
     5161 tcacttaggg gcagggcaag ttggctgcta atctcattaa agagttcctg gtgccaaagg
     5221 gagtaatcta ggctggtggc cgtggttttg ttttcaaagt tactttgaag ataaatatcc
     5281 cgcagggagt gatagacaac atcaaaagag tctccctttt catggaccag ggtaaccagg
     5341 ccttgttttt gccagtagcg ataggcttct gctacctggc ggtattctat ttctaagagc
     5401 ttggctagac tggctagatt ggcttctttt ttttcttggg caaaacgaag gccaagtaga
     5461 tagaccttaa gttcaatcag cccacaatag ggcataaatt cgtcaataaa taggtgttct
     5521 atgctagtta cgccaaagtc gaaactagtt ggttttagct caatattcat gctactacct
     5581 cctttgtcat tatactgata aaagcagggg gtggcaagta aagcccaggg gactttttat
     5641 gctggttttt aacttataga taaaaaggat tgacgaattg tatatacagg gatatactat
     5701 aaata
//
LOCUS       DASRNA010000116         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_999458,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000116 DASRNA010000000
VERSION     DASRNA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999458"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..1408
                     /gene="glpX"
                     /locus_tag="VFD08_03075"
     CDS             425..1408
                     /gene="glpX"
                     /locus_tag="VFD08_03075"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004817507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZK10032.1"
                     /translation="MDRDLGINLARVTEAAALASAKYLGKGKAEKADEVAVEQMRIMF
                     DILNINGEVVIGEGEIDEAPMLYIGEKVGDGSYSQVDIAVDPVDGTTSLANGMDNAIS
                     VIAVAPRGCLLNAPDVYMNKIACGPRARGAINLSASATENIKKVAQRLNKDVSDITVS
                     VLKRERHDELIKEIRATGARIRMVTDGDIFTAIATGFSAGKIDLMMGIGGAPEGVLAA
                     AALKCLDGDFQGQFTPLNPAQKKRCDLLNVDLDKIYTLDDLVKGNQVLFAATGISSGD
                     FLQGVEFLEGNTATTESLVLRKPSGTTRFIKSIHKLDQKLSDVDPKYLKRG"
BASE COUNT          725 a          361 c          485 g          637 t
ORIGIN      
        1 ctatgataag tctttctagc caaggtggtt ttatagacac ctctctttgc tgagtttgcc
       61 ttgcgacagg gctagaaaaa caccttgttt tattatgaag aaattaagtg aacttacaat
      121 aaaggcctag aaatatgata agatagaggc agaaagactt atactacata tttgaaaaaa
      181 ctaagtatag aaggcaatat tccgctggag tttttacgag gtcttaggca aaatcattaa
      241 actcccaggg gggccaatct atagcaaagg taaccacctt atatgactat tacttgtatg
      301 agaagaactc atcccggact agggcaaaag aggcaaagaa gaaggcgcgt attggtaaag
      361 aaaaacagga ctttagtaaa actacataga aaagcatctt tatattagat gggaggccta
      421 aatgatggat agggacctgg gtattaactt ggcaagagtt actgaagctg cagcactcgc
      481 aagtgcaaaa tatcttggaa aaggcaaggc ggaaaaagca gatgaagtcg ctgttgaaca
      541 aatgagaatc atgtttgaca tattaaatat aaacggcgag gtagtaatag gggagggaga
      601 aatcgacgaa gctcccatgt tatacattgg agaaaaagta ggcgatggat cttatagtca
      661 agttgatata gcggtagatc ctgttgatgg aaccacatct ctagcaaatg gtatggataa
      721 tgctatttct gttattgcag ttgcaccaag gggatgttta ttaaacgctc ccgatgtcta
      781 tatgaataaa atcgcatgcg gtcctagggc gaggggagct ataaacctat ctgctagtgc
      841 aactgaaaac ataaaaaagg tagcccagag attaaataag gatgtttctg atataacggt
      901 atctgtttta aaaagagaaa gacatgatga gctgattaaa gagatcagag ccacaggggc
      961 taggattaga atggttaccg acggggatat ttttactgcc attgccactg gctttagtgc
     1021 aggtaaaata gatttaatga tgggaattgg aggagctcct gagggggttc ttgcagcagc
     1081 agccctaaag tgtttagatg gagattttca gggacaattc actcctttaa accctgctca
     1141 aaaaaagaga tgtgatttac taaatgtaga cttagacaaa atctatacct tggatgattt
     1201 agttaaggga aatcaagttt tgtttgcagc cacaggaatt tcgtctggag actttttaca
     1261 gggagttgag ttcctggagg gcaatacagc cacaaccgaa agtttagtcc taagaaaacc
     1321 aagtggcact acaagattta taaagagtat tcataaactc gatcaaaagc taagtgatgt
     1381 agatcccaag tacttaaaaa gaggctaggc ctcttttttt gttttttttc tttagtcaga
     1441 gaaatctttt ttcttgtgat tgagtttatc ctccttgtca actaggcctg gatatggccc
     1501 tttatctttg gtttcagacc aaggatattt tttaatctca atgtttatta gaggagatgt
     1561 tcttctttcc attttggatg aaagggctca agttcacgca ggcccccttc tcatatgata
     1621 atctttagaa aacacaggag tcacatcaaa actgcccatg caaaaagagc tactcgatgg
     1681 ctgggtatct acaagattgg tgggagaagg cgccctttgg ctttttaagt agcaaaaatt
     1741 gtaagcttaa actcgtctac agtcatcaaa gcgactctta aatccactag ttctcttgtg
     1801 gctcttgtac ttatctacct actttatgct gtttttatga ttttattaat acttttctta
     1861 gataaactgt aaattgtctt cctagcgact cttaaatcaa attccaaatg gcttgttttt
     1921 ttaacaaaaa gaagatcgaa tcagattata tatcctaagt gtgtttgctt atgaaagaat
     1981 gtgtggttaa aaaggccgta tgactgttta acttaataaa aaaagagtcc caagctacag
     2041 attttagcct agttaagcct tattgagaaa aaaccccacc ataagggggg gggtgtttga
     2101 tttttaaccc acatacagag aaggcggaag ttaagtaatt gcaagggttg cataaaaatc
     2161 actgtgatat aatggacgcg agaacatagg taaatagcag taatacat
//
LOCUS       DASRNA010000117        15093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_344320,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000117 DASRNA010000000
VERSION     DASRNA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15093
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344320"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..344
                     /gene="secG"
                     /locus_tag="VFD08_03080"
     CDS             96..344
                     /gene="secG"
                     /locus_tag="VFD08_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZK10033.1"
                     /translation="MSTPTLIFTIIFLIASLVLIVSVLMQSGKSSGLSGSIGGGAEQL
                     FGKQKGRSMDAMFEKMTSAAAVVFMISAIVLLILQKYN"
     gene            359..2317
                     /locus_tag="VFD08_03085"
     CDS             359..2317
                     /locus_tag="VFD08_03085"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZK10034.1"
                     /translation="MMDFLPIITGVIALAFTYYLIVTKINPVSPGNDKMIEIAGHIQD
                     GAMAFIKREYTYIAVFSIILMLLLAIGLNIATAVAFVIGTVLSALAGFIGMRISTRAN
                     VRTASAAMSDGMNRALDVAFGGGAVMGMSVVGLGFLGLGLVLLIYPGRYDIVTGYSLG
                     ASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGM
                     GADLFESYVGSIIASLTLGVSLGIAGAVEFPIALAAVGIFASIIATFFVKGNEKSDPQ
                     KALTMSTYVSSAIVAVAALFLSNYYFGSFDASFAIMAGLVSGLLIGQSTEFYTSAEHK
                     HVKQIAEQSQTGAATTIISGLSVGMLSTVLPILFISGAILVAHHFQGLYGIALAALGM
                     LSTTGITVAVDAYGPIADNAGGIAEMSNLPESVRVITDKLDSVGNTTAAIGKGFAIGS
                     AALTALALFASYAQAVGLEGIDILNSKVTVGLFIGGMLPFVFSALTMNSVGKAANHMI
                     EEVRRQFKEIPGIMEYEATPDYARCVDISTASALREMIIPGVMAVVAPLVVGKVLGPA
                     ALGGLLAGALVTGVLMAIFMANAGGAWDNAKKHIEEGNFGGKGSEAHKASVVGDTVGD
                     PFKDTSGPSLNILIKLMTIVSLVFASIF"
     gene            2419..3189
                     /locus_tag="VFD08_03090"
     CDS             2419..3189
                     /locus_tag="VFD08_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HZK10035.1"
                     /translation="MKLIVFAGSAEARRWIHSGRRQNYEQWIFLKNEYGRDLLPEENE
                     KLKIFAGEKSKEDIEALLPRDSLAIIDGHHPFSYEFSKELKELASNRGIEYLRLVEEE
                     KAPRGVVSVDSYEEASRYLERSKGNILLTMGSQSPKSFMSPRLHLRIYARIEPSLEAL
                     QEALELGLGYKQILALQGPFSQEMNLAMIDQYKIKIMVTGESGLQEGVEEKAIAARKA
                     GITLLVIRDPMEKRYSLYEIEKEIEKLAKIYKRQASKS"
     gene            complement(3186..3401)
                     /locus_tag="VFD08_03095"
     CDS             complement(3186..3401)
                     /locus_tag="VFD08_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10036.1"
                     /translation="MQRKFLRSLFIHIIILILLFLLLFFLESMELIKTKSALTYFFIA
                     FFFVLIDLFLSSRLQKYLQDTKDKSKD"
     gene            3487..3861
                     /locus_tag="VFD08_03100"
     CDS             3487..3861
                     /locus_tag="VFD08_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10037.1"
                     /translation="MFPSYEEMGEVLDSLAEELPQVFFEGLNLGVVLFEDLKVHHQAV
                     NEDLIILGEYHRTKLGRQIRIYYRSFRRVYGHLEYEELVEELRETLRHEFTHHIQDLA
                     GDKSLEIEDGLKLAKWEERRKK"
     gene            3858..4922
                     /locus_tag="VFD08_03105"
     CDS             3858..4922
                     /locus_tag="VFD08_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HZK10038.1"
                     /translation="MNLERMIDLSNCFGPAGFEEEVARLIKKSYKGSKKLTSDTLNNV
                     YIGLEEKKGLRVQLDAHLDEVGFMVSRVFENGMLGLHPLGGWVEYNLPSQSFLIRSKS
                     GEFIPGIVASKPPHFLSEKERNTHLALEDMVVDVGATSAREIAGLGIGIGSPMVPQVS
                     CQKFIERDLLLGKAFDCRLGVAALLDVVEVLEKEEVDLEIIGALSSQEEGGLRGAQVT
                     ARQVEADLAIVFEGCPADDSFLSPPQSQTGIHKGPMLRHVDRGMLTHPGFQNFALEIA
                     KSKMIPHQEAVRSGGSTNGSPIHLSHKGIPTIVLGIPVRYIHSHFGYASLRDYQATVE
                     LALEIIRGINKEVYDSFKPF"
     gene            complement(5007..5564)
                     /locus_tag="VFD08_03110"
     CDS             complement(5007..5564)
                     /locus_tag="VFD08_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZK10039.1"
                     /translation="MIYLQLFLAFVQIGMFSVGGGYAALPLIKEQVVTNYQWLSATEF
                     YDIVTIAEMTPGPIAINAATFAGIKIAGIPGALLASFSTILPSMIIVTFLAFILSRKR
                     GSSVRILEKVLYGLRPAVVALIASAALSLMQVALLQESQIDFYKTGLFLLGLYLLRKK
                     KITPIKLLFLSGSIALIYWQALLLI"
     gene            complement(5561..6097)
                     /locus_tag="VFD08_03115"
     CDS             complement(5561..6097)
                     /locus_tag="VFD08_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZK10040.1"
                     /translation="MNSMNRREIFLTALRFSCTTFGGGFVMISMMEKEFVEDLGILPR
                     EEMMDIAAIAQSAPGAVTVNAWILLGYRMAGLTGSLVAILGAILPPILIVGAISFFYE
                     RFIASSFISVGMKGMQVAVAAIIVDLVITLFKSLTKDGKIWDFILVILALVLTGYFHF
                     PIYILLISYAIIGIWRKA"
     gene            6469..6834
                     /locus_tag="VFD08_03120"
     CDS             6469..6834
                     /locus_tag="VFD08_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HZK10041.1"
                     /translation="MKIITNNPRVLDKHCEHYDVHFVCIGYLDLLELVRDRIHKGARL
                     LTHPLSGSVKPNETPYKSIVIEEGEDGVDFKSLMIIEDAIVMSRNLLKTPKLFLEDDK
                     RMEDCRVIDLTLLDSALEK"
     gene            6851..7795
                     /gene="trxB"
                     /locus_tag="VFD08_03125"
     CDS             6851..7795
                     /gene="trxB"
                     /locus_tag="VFD08_03125"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003490434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZK10042.1"
                     /translation="MSKVYDVIIVGAGPAGLSSAIYTGRSKLDTLLLEKEKAGGQIVT
                     TDEIENYPGSPQDESGPKLIGRMVEQVNNFGVDLRYEEVVEFDFSDKIKLIKTNKEEY
                     KTKSVILANGAVPRKLGFPGEAEFTGRGVSYCATCDAAFFEGLEIYVIGGGDTAVEEA
                     VYLTRFARKVYIVHRRDELRAVQSIQDKAMANDKIEIIWDSVVKEVSGEGGVVDRILL
                     HNRVTGEDTEHLADEDDGILGVFVLVGYLPETGIYKDTITLNNSGYVEADEGLHTNIE
                     GVFAAGDIRNKPLRQVVTATSDGAVAAIQAEKYIEDNF"
     gene            7804..8121
                     /locus_tag="VFD08_03130"
     CDS             7804..8121
                     /locus_tag="VFD08_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010778953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZK10043.1"
                     /translation="MLELDKNNFGPEVLESTGAIMVDFWGQSCEPCKALMPHILALEE
                     KYGDKITFASFDIAKGRRVAMSQGVAGLPAIYIYKDGVMVEDLRGDAATATAVEELIQ
                     RHI"
     gene            8143..9429
                     /locus_tag="VFD08_03135"
     CDS             8143..9429
                     /locus_tag="VFD08_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HZK10044.1"
                     /translation="MQLELGNIFIHDVQFASKTHVKDGVLYVNKEELEALIREDEHIT
                     DVQIDLARPGESVRITPVKDVIEPRVKVEGPGGIFPGVINKVNQVGTGRTHVLKGAAV
                     VTAGRIVGFQEGIIDMTGPGAEYTPFSRLNNVVLTMDVKEEILQHAHEVILRYAGLKA
                     ALYLGEAARELEPDELRSFETLPLLESVKKYPDLPKVAYVQMLQSQGLLHDTYVYGVD
                     AKKTISTFIYPTEIMDGAIISGNCVSACDKNTTYHHLNNPVVDDLFEKHGKELNFVGV
                     IITNENVYLADKIRSSDWTSKLAGYMGVDGVVISQEGFGNPDTDLIMNCVKIEDKGIK
                     TVIITDEYAGRDGASQSLADADAKADAVVTGGNANEVIELPPMDKVIGDVSVANVIAG
                     GFDGSLREDGSILVELQAITGATNELGFNKMSAKGY"
     gene            9464..9934
                     /gene="grdA"
                     /locus_tag="VFD08_03140"
     CDS             9464..9934
                     /gene="grdA"
                     /locus_tag="VFD08_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_093314543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:9593..9595,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HZK10045.1"
                     /translation="MSLYDGKKVILIGDRDGIPAPAMEECLKSTDAEIVFAATECFVU
                     TAAGAMDLENQRRVLDLTNEHGAENVIVVLGGAEGEAAGLAAETVTAGDPTFAGPLAG
                     VQLGLRVYHCCEPEFKNEVDSEVYDEQIGMMEMVLDVDDIISEMSAIREEFTKY"
     gene            9951..11258
                     /gene="grdB"
                     /locus_tag="VFD08_03145"
     CDS             9951..11258
                     /gene="grdB"
                     /locus_tag="VFD08_03145"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:10998..11000,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HZK10046.1"
                     /translation="MSKIKVVHYINQFFAGIGGEEKAHVEPELREGIVGPGMAINAAL
                     GEEAEIVATVICGDSYFNENLESAKETVLAMIKSQDADLFIAGPAFNAGRYGVACGAI
                     TAAVKEELGIPSLTGMYEENPGVDMYKKEVYIVATRDSAAGMRDAVKKMVPLALKLAK
                     NEEIDEPDVEHYFPRGIRKNFFTQQRGSSRAVDMLVKKLKGEEFTTEFPMPSFDRVEP
                     AAALADVRKAKIAIVTSGGIVPKGNPDRIESSSASKYGEYDIEGVMDLTEETYETAHG
                     GFDPVYANLDADRVIPVDVLRQYEKEGKIGELHRYFYSTVGNGTAVASSKAFSKEIGA
                     KLIEDGVDAVILTSTUGTCTRCGATMVKGIEEFGIPVVHMCTVVPISLTVGANRIVPT
                     VAIPHPLGNPNLDAQEELALRKRLVDKALAALETEVEEQTVFE"
     gene            11316..12848
                     /gene="grdC"
                     /locus_tag="VFD08_03150"
     CDS             11316..12848
                     /gene="grdC"
                     /locus_tag="VFD08_03150"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HZK10047.1"
                     /translation="MMFPVLKAASYVLMHAPDMVLKNGTTQTTERILNPESEYLTKVN
                     ENLRPFEEIVSYPANQCYIGNITPEDLATYEMPWYDKAVEGAKAQGKKGEIFAEDVFI
                     ALIKASDAFDLVLLEKDFTEAVRPKLDAYTGLNAKAKTLPKGAELERIQTEIEKRDAE
                     PLLHKGEVVGCVKKAHDIDINLNAHVILENLVVKASGTMAMERLFVLNNIDPATVDYV
                     IECSEEACGDMNQRGGGNFAKALAEMTDCLNATGSDTRGFCAAPTHALIEAAAFVKSG
                     VFKNVVVVAGGSTAKLGMNGKDHVKKGMPILEDVVAGFALLVSENDGQHPILRTDLVG
                     GHTVGTGSSPQNVMSALITKALDKGDLKITDIDKYSVEMQNPDITKPAGAGDVPQANY
                     KMIAALGVMRKDLERSQLNSFVQTHGMVGWAPTQGHIPSGVPYCGFAIDDIMSEKIKR
                     AMIVGKGSLFLGRMTNLFDGVSIVVEKNPGIQEEEGSASKEEIRTMVAQAMRDFAASL
                     NQ"
     gene            12857..14023
                     /gene="grdD"
                     /locus_tag="VFD08_03155"
     CDS             12857..14023
                     /gene="grdD"
                     /locus_tag="VFD08_03155"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HZK10048.1"
                     /translation="MENLKVKAIIADVFEEIAQGLETGLFHDKVALGLTLYGSELGAS
                     NLLKGAETFAKDNADVEVVLIGPKVETDLTLIEVESEELQYDKMEELLDSGRIQGCVT
                     MHYSFPIGVSTVGRTLTPGFGKEMYVATTTGTSSAHRNEAMVKNAISGIIAAKAAGIQ
                     EPSLGILNVDGARTVERALKELSAGGYPINFADSDRSDGGCVMRGNDLLRGTPDIMVT
                     DTLTGNILMKTFSSYHTGGNFEAVGDGYGPGIGQDFDRLVLILSRVSGSPVVANALAY
                     AKDLVLGDVHKLSKKEYKKAKDAGLEEILAGLTKTKEKAQEEEVTAPAEEITTGEISG
                     IDILELEDAVRVLWKEGIYASSGMGCTGPVVMVNESKIGVASQILAAAGYVSED"
     gene            complement(14236..14679)
                     /locus_tag="VFD08_03160"
     CDS             complement(14236..14679)
                     /locus_tag="VFD08_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10049.1"
                     /translation="MALYGYKGETLLRGKIEAERVTEIPKDIPWQAYQTALHLEGVTI
                     EAKYQKAVKTSCKKLMSLTSEDPYNLYEEDEDGIYCFMCMPILASRDDFKKFIHHSSV
                     LKRHIEEDEFFNSAPYFYNQSNDEPKILYLDVKKDGSCHYYISRA"
BASE COUNT         4584 a         2654 c         3503 g         4352 t
ORIGIN      
        1 taacttctag atgagaatgg tattttatga ctttttttat gttgccaagg gatgcctact
       61 atgataacat taatctgtta tgggaggagg tatttatgag tacaccaaca ctgattttta
      121 caatcatatt tttaatagct agtcttgttc ttattgtcag cgttttaatg cagtcaggca
      181 agtcgtcggg attaagcgga tccattggtg gcggcgccga gcagcttttt ggcaaacaaa
      241 aggggcgctc tatggatgct atgtttgaga aaatgacttc agctgcagcc gtagttttta
      301 tgataagtgc tatagttctt ttgatattac aaaagtataa ttaattgaga ggtaaaaaat
      361 gatggatttt ctgccaatta ttactggggt gattgccttg gcatttacct actatttgat
      421 cgtgacgaaa attaatcctg taagtccagg aaatgacaag atgattgaga tagcaggaca
      481 tattcaagat ggagccatgg cttttattaa gcgtgaatat acctatattg ctgtgttttc
      541 tataatctta atgcttcttt tagctatagg tttgaatata gctaccgcag tagcctttgt
      601 tattggtact gtgctttctg ctcttgctgg ctttattgga atgcgaattt ctacccgtgc
      661 taatgtgcgt actgcttctg cggcaatgag tgatgggatg aaccgcgctc tagatgttgc
      721 ctttggtggt ggtgctgtta tgggtatgag tgttgtaggc ctaggttttc taggacttgg
      781 tcttgtttta cttatttatc ctggtcgata tgatatcgtt acaggatata gcttaggtgc
      841 ttcttctatt gctttatttg ctcgagttgg tggtggtatt tatacaaaag ccgctgatgt
      901 tggagcagac cttgtaggta aagtcgaagc tggtatacca gaagatgacc caagaaatcc
      961 cgctgttatt gctgataatg ttggagacaa tgttggtgat gttgccggta tgggtgctga
     1021 cctttttgaa tcttatgttg gtagtattat tgctagttta accctagggg ttagtttagg
     1081 cattgctgga gctgttgaat ttcctattgc tcttgctgct gttggtattt ttgcatctat
     1141 tattgcaaca ttttttgtta aaggaaatga aaagagcgat ccacaaaaag cactaaccat
     1201 gagtacatat gttagtagtg ctatcgtagc tgtagcggct ctatttttaa gtaattatta
     1261 ttttggaagt tttgatgcat cctttgctat tatggctggt cttgtttcag gtcttctaat
     1321 cggacaatca acagagtttt atacatcagc tgaacataaa cacgtaaagc aaattgctga
     1381 gcaatcccaa acgggagcag ctacaacaat tatcagtggt ctttctgtgg gtatgctctc
     1441 aactgtcctt ccaattcttt ttattagtgg agctatttta gttgcccacc atttccaggg
     1501 cctatatggc attgcccttg ctgctcttgg aatgcttagt accacaggca ttacagttgc
     1561 tgtggatgca tacggtccta ttgctgataa tgccggaggt attgccgaga tgagcaatct
     1621 tcccgagtca gtacgtgtaa ttacagataa acttgactcg gttggaaata ctacagcagc
     1681 aattggtaaa ggttttgcta taggttctgc tgctttaacg gctctagcac tttttgcttc
     1741 ctatgcccag gcagttggcc tagagggtat tgatatttta aattctaaag taacggttgg
     1801 tctttttatt ggtggtatgc tgccctttgt tttctctgct ctaacaatga attcagttgg
     1861 aaaagctgcc aatcatatga ttgaagaagt gcgacgtcag tttaaagaaa ttcctggtat
     1921 tatggagtat gaagcaacac ctgattatgc tcgttgtgtt gatatttcaa cggcctccgc
     1981 tcttagagaa atgattattc caggagttat ggctgtagtt gctcctctag ttgttggtaa
     2041 agttcttgga ccagcggctt taggtggact tcttgcagga gctcttgtaa cgggtgttct
     2101 tatggctatc tttatggcca atgccggcgg tgcttgggat aatgcaaaaa aacatattga
     2161 agaaggtaac tttggtggaa agggctcaga agcccataaa gccagtgttg ttggagatac
     2221 tgtaggtgat cctttcaagg atacatctgg tccatcactt aatattctta ttaagctgat
     2281 gactattgtg tctttagtat ttgcttcaat attctagaaa aagccccttt tggggctttt
     2341 tttattgacg ataaagagat catagagtag actttttagt gttaagattg agctaaagat
     2401 gactgagatg aggtaggaat gaaattaatt gtttttgctg gtagtgcgga agctagaaga
     2461 tggatacata gtggccggcg acaaaattat gagcaatgga tatttttaaa aaatgagtat
     2521 ggtagggatc ttcttccaga agaaaacgaa aaactaaaaa ttttcgcggg agagaaaagc
     2581 aaagaagaca ttgaagctct tcttcctagg gacagtttgg ctattattga tggacaccat
     2641 cctttttcct atgaatttag taaggagtta aaagagcttg ctagtaatag gggaattgaa
     2701 tacctacgac tggttgaaga agaaaaagct cccagaggag ttgtatctgt agatagctat
     2761 gaagaagcaa gccgctatct tgaaagatca aagggtaata ttcttttaac catgggaagt
     2821 caaagcccaa agagttttat gagcccaagg cttcaccttc gcatatatgc ccggattgaa
     2881 ccaagcctag aagcactaca agaagccctt gaacttggtc ttggatacaa gcaaattctg
     2941 gcactgcaag gaccctttag ccaggaaatg aatttggcta tgattgatca gtataaaatt
     3001 aaaatcatgg ttacgggaga aagcggccta caagaagggg tagaagaaaa ggctattgca
     3061 gcaagaaaag caggtatcac cctgcttgtt atacgcgatc ctatggaaaa aagatactca
     3121 ctatatgaga tagaaaaaga aatagagaaa ctggcaaaga tctataaaag acaggccagt
     3181 aaaagttagt ccttgctttt atcttttgta tcttgaagat atttttgcaa cctagaagat
     3241 aggaaaagat caatcaacac gaagaaaaaa gcaataaaaa aatacgtaag ggctgatttt
     3301 gtttttatta attccatgct ctcaagaaaa aagagcagta aaaacaaaag gataagtatg
     3361 attatatgaa taaacaagga gcgcaaaaat tttcgctgca ttagaataac ctccttaggt
     3421 agatctctac ttagtttatc atagattatg aaaaactaaa ttgtcttggg caagaaagga
     3481 gatgaaatgt ttcctagcta cgaagaaatg ggagaagttt tggattcctt ggcggaagaa
     3541 cttcctcaag ttttttttga gggattaaat ttgggagtag ttctctttga ggatcttaag
     3601 gttcatcatc aagcagtcaa tgaggacctt attattttgg gagaatacca ccgcactaaa
     3661 ttaggcaggc agatacggat ttactatcgc tcttttcgcc gtgtttatgg acaccttgaa
     3721 tatgaagaac ttgttgagga gttaagagaa actcttcgtc atgaatttac tcatcatatt
     3781 caagatttgg ctggggataa aagccttgaa atagaagatg gccttaagct tgctaagtgg
     3841 gaagaaagaa ggaaaaaatg aatctagaaa gaatgattga tcttagtaat tgttttggtc
     3901 cagcgggctt tgaagaagaa gtcgctcgac taattaaaaa aagctataag ggaagtaaaa
     3961 agcttactag cgacacacta aacaatgtat atataggcct agaggagaaa aaagggctca
     4021 gggtacaatt agatgctcac ctagatgaag tgggctttat ggtctctagg gtctttgaaa
     4081 acggtatgct aggccttcat ccccttggag gatgggtaga atataattta ccctcccagt
     4141 cttttttaat tcgctctaaa agtggcgaat ttattcctgg tattgtggca tcaaaacccc
     4201 ctcattttct aagtgaaaaa gaaagaaata cccatctggc cttagaagat atggtggtag
     4261 atgttggagc tacaagcgcc agggagattg ccggccttgg cataggcatt ggatctccca
     4321 tggtgcccca agtgagctgc caaaagttta ttgaaagaga cctattactt ggaaaggctt
     4381 ttgattgtcg cttgggggtt gcggctcttt tagatgttgt ggaagttttg gaaaaagaag
     4441 aagttgatct tgaaataatt ggggctcttt ccagccaaga agaaggaggg ctcagagggg
     4501 cccaggttac agctaggcag gttgaggctg atcttgctat tgtctttgaa ggttgcccag
     4561 cagatgatag ttttctttcg ccaccccaat ctcaaacagg aattcacaaa ggccctatgc
     4621 tgcgccatgt tgaccgcggt atgcttaccc atccgggctt tcaaaacttt gcccttgaaa
     4681 tcgcaaaaag caaaatgatt ccccaccaag aagctgtacg aagcggggga agtacaaatg
     4741 gttctcccat tcatttgagt cataagggaa tacccactat tgtccttggg attcccgttc
     4801 gttatattca ctcccacttt ggatatgcca gtcttcgcga ttaccaggca acagtagaat
     4861 tagcccttga gattattagg gggataaata aagaggtata tgattctttt aaaccctttt
     4921 aaagaaaaaa gccagtgatg tgtttacact ggctttctta cttacttagg gcaataaact
     4981 tacagggatt ttttgaactt tgccagctag ataagcagta aagcttgcca gtatattagg
     5041 gcaatagatc ctgataaaaa caggagttta ataggggtga ttttcttttt tcgaagaaga
     5101 taaaggccca gtagaaagag tcctgtttta taaaagtcta tttgagattc ttgcagcaag
     5161 gctacctgca taagactaag ggcagcagaa gcaatcaggg caacgactgc cgggcgtaaa
     5221 ccataaagta ctttttctaa gattctaaca ctgcttcccc gcttgcgcga aaggataaag
     5281 gcaagaaagg tgacaataat catagagggc aggatagtag aaaagcttgc tagtaatgct
     5341 ccagggattc cagcgatttt aattcctgca aaggttgcag catttatggc gataggtcct
     5401 ggtgtcattt ctgctatggt aacaatatca taaaactctg tggcgctaag ccactgataa
     5461 tttgttacca cttgctcttt aattaggggt agtgccgcat accctccccc tacgctaaac
     5521 attcctattt gtacaaaggc taaaaaaagt tgtagataaa tcatgccttc ctccagatgc
     5581 cgataatggc ataagaaata aggaggatat atattggaaa atgaaaatag cctgttagga
     5641 ctaaggctaa aataactaag ataaaatccc atatcttgcc gtcttttgta agggatttga
     5701 aaagagtaat aactaaatct acgataatag cagctacagc aacttgcatg cccttcattc
     5761 ctacagaaat aaagctactt gcaataaagc gctcgtagaa aaaggagata gcccccacaa
     5821 tcaaaatagg tggcaatata gcccctagga tagcgactag acttcctgtt agaccagcca
     5881 tacggtagcc taataaaatc caggcattaa cagtaaccgc tcccggagcg ctttgagcaa
     5941 tcgccgcaat atccatcatt tcttcccgag ggagtatgcc taggtcttcc acgaattctt
     6001 tttccatcat actaatcatg acaaaaccgc caccaaatgt ggtgcaacta aatctaagtg
     6061 ccgtgagaaa tatttctcgt cggttcattg agttcatagg acctccttaa attagtatac
     6121 aatataaagc tttgctcttc tataagataa atccaattat ttttgatttc caattgatgg
     6181 cagctgcaaa agagtataaa ataggatgtc ttttttattc ttagaaggag caattttgct
     6241 atggaaagct ttttttcata gcaggttcgc aaaaaaagcc ttatagaaga caaaagagaa
     6301 aagaatgtaa aatcagcgga aaaactcaac ttattatccg ctaagttttc gctgaaatgt
     6361 tatatatttg ttgggaatat atggtgtttt gccctaggat ttttagcctt ttatgatata
     6421 ctgtcaatgt caggtaaaaa tgaagaattt tgtccaatgg gagcgtttat gaagattatt
     6481 accaacaacc ctagggtatt agataagcac tgtgagcatt atgacgttca ttttgtttgc
     6541 ataggatact tggatcttct agaactagtt cgagatagga ttcacaaagg agcccgtctc
     6601 ctaactcatc cattgagtgg aagtgtaaag ccaaatgaaa caccctataa atccattgtg
     6661 attgaagagg gggaagatgg agtggatttc aaatctctaa tgattataga ggatgcgatt
     6721 gttatgtcga gaaacttgct gaaaacgcca aagctttttc ttgaagatga caaacgtatg
     6781 gaagattgta gggtaattga tcttacctta ctggatagtg cattagaaaa atagaaagga
     6841 caaataaccc atgagtaaag tttatgatgt aattattgtt ggtgctggac ctgctggttt
     6901 atcttcggcc atctatacag gccgctcaaa gctagatacc ttattattag aaaaagaaaa
     6961 agctggtggc caaattgtca ctaccgatga aatcgaaaac tatcctggtt caccccagga
     7021 cgagagtgga ccaaaactaa ttggtcgaat ggtcgaacaa gttaataact ttggtgttga
     7081 tctgcgttat gaagaagtag ttgaatttga ctttagtgat aaaataaagc tgataaaaac
     7141 aaataaagaa gaatataaga ccaagtccgt aatccttgct aatggagctg ttccacgtaa
     7201 acttggattt cctggagaag cagaatttac gggaagaggt gttagctatt gtgccacttg
     7261 tgatgcagcc ttttttgaag gtcttgaaat ctatgtaatt ggtggaggag atacggctgt
     7321 tgaggaagca gtttatttga ctcgatttgc tcgcaaagtc tatatcgttc atagacgtga
     7381 tgagctccgt gcagttcaga gtatacaaga taaggccatg gccaatgata agattgaaat
     7441 catttgggat agtgtggtaa aagaagttag cggtgagggt ggagttgttg atcgaatcct
     7501 acttcacaat cgcgttacag gagaagatac agagcattta gcggatgaag atgatggaat
     7561 attgggagtt tttgttctgg tgggatatct acctgagaca ggaatttata aggataccat
     7621 cacactaaat aatagcgggt atgtcgaagc cgatgaaggt ctgcacacaa atatagaagg
     7681 tgttttcgca gccggagata tccgtaacaa accacttcgt caagttgtta ctgccactag
     7741 tgatggagct gttgctgcca tccaagccga aaaatacatc gaagataatt tttaggagga
     7801 aaaatgttag aactggataa gaataatttt ggtcccgaag tactagaatc cacaggtgca
     7861 attatggttg acttttgggg acaaagctgt gaaccatgca aggcgctaat gcctcatatt
     7921 ctagctttag aagaaaaata cggcgataaa atcacatttg cttcatttga tattgcaaag
     7981 ggtcgtcgtg ttgccatgtc tcaaggcgtt gctggacttc ctgcaatata catttacaaa
     8041 gatggagtaa tggttgaaga tcttcgagga gatgctgcta ctgcaactgc tgtggaagaa
     8101 ctaatccaaa gacacattta accaagtgaa aggaggtggg ctatgcaact tgaattaggc
     8161 aacattttta ttcatgatgt tcagtttgca tcaaagaccc acgtaaagga tggtgtgctc
     8221 tatgttaata aagaagagct agaagcactt atccgtgaag acgagcatat taccgatgta
     8281 cagattgatc ttgctagacc aggcgagagt gtacgaatca ctcctgttaa agatgtaatc
     8341 gaacctcgtg taaaagttga aggtcctggt gggattttcc caggagtaat caacaaagtc
     8401 aatcaagtag gtactggaag aacacatgtc cttaaaggag cagctgttgt tactgctggg
     8461 agaatcgttg gtttccaaga gggaattatt gatatgactg gcccaggagc tgagtacaca
     8521 cctttttcaa gactaaataa tgttgttctt acaatggatg taaaagaaga aatattacag
     8581 catgcccacg aagtaattct tcgctatgca ggcttaaaag cagctcttta tctaggtgag
     8641 gccgctcgtg aactagagcc tgatgaactt agaagcttcg agacgctgcc actattagaa
     8701 tcagttaaaa aataccctga tctacccaag gtagcttatg tgcaaatgtt acaatctcaa
     8761 gggcttctgc atgacaccta tgtttatggg gttgatgcga agaaaacaat ctcgaccttt
     8821 atttatccga cagaaattat ggatggcgct ataattagcg gtaactgtgt ttctgcttgc
     8881 gataaaaaca cgacttatca tcacctcaat aatcctgtgg tagatgattt gttcgaaaag
     8941 cacggtaagg aactgaactt tgttggtgta attattacca atgaaaacgt ttatcttgcc
     9001 gacaaaatcc gctcctctga ctggactagt aaactagccg gttatatggg tgtggatggc
     9061 gtcgttatct ctcaagaagg ttttggaaat cctgacacgg atcttattat gaactgcgtg
     9121 aaaatcgaag ataagggcat taagactgtt attattaccg atgaatatgc gggtcgtgac
     9181 ggagccagtc aatctctggc agatgctgat gcaaaggctg atgccgttgt tacaggagga
     9241 aatgctaatg aagtgatcga acttcctcct atggacaagg taataggtga tgtttctgtt
     9301 gcaaatgtta tagccggtgg atttgatggt agcctccgtg aagatggaag cattctggta
     9361 gagctacaag caattaccgg agctaccaat gaacttggct ttaacaaaat gagtgccaaa
     9421 gggtactaaa atcaaaaagg aaaaaaagaa aggaaatatt tagatgagcc tatatgatgg
     9481 aaaaaaagtt attctcatcg gcgatagaga tggtattcca gctcccgcca tggaagaatg
     9541 tctaaagtcc actgatgcag aaatcgtctt tgcagctact gaatgttttg tgtgaacggc
     9601 tgcaggagct atggacctgg agaatcaaag aagagtattg gatctaacca atgagcatgg
     9661 tgccgagaac gtaattgttg ttcttggtgg tgctgaaggt gaagccgccg gactggcagc
     9721 tgaaaccgtt acagcaggcg atcctacttt tgcaggtccc ttagcaggag tccagttggg
     9781 actcagagtt taccactgct gcgaaccaga atttaagaat gaagtagatt ccgaagttta
     9841 tgacgagcaa atcggcatga tggaaatggt cctcgatgtt gatgatatta ttagcgaaat
     9901 gagtgctatt cgagaagaat ttacaaaata ctaagcgtta ggagagagat atgagcaaga
     9961 tcaaggttgt acattatatc aaccagttct ttgcaggtat cggaggggaa gaaaaagccc
    10021 atgttgaacc cgagttaaga gaaggtattg ttggtcctgg aatggccatt aatgcagctc
    10081 ttggggaaga ggctgaaata gtcgctacag taatttgtgg cgactcctac ttcaatgaaa
    10141 acctggaaag tgctaaagaa acagttttag cgatgattaa gtctcaggat gcggatctgt
    10201 ttattgctgg tcctgccttt aacgctggac gttatggcgt tgcctgtggt gcaattactg
    10261 ctgcagttaa agaagagtta gggatccctt ctcttacagg aatgtatgaa gaaaaccctg
    10321 gcgttgacat gtataaaaaa gaagtttata tcgtagctac tagagatagt gctgctggta
    10381 tgcgagatgc tgttaaaaaa atggttcctt tggctcttaa attagcgaaa aacgaagaaa
    10441 ttgacgagcc agatgtggag cattacttcc ctagaggaat tcgcaagaat ttcttcactc
    10501 aacagcgtgg ctcaagccga gctgtggata tgctagtgaa aaaactcaaa ggtgaagagt
    10561 ttactacaga atttcctatg cccagttttg accgcgtaga acctgctgcg gctctagctg
    10621 atgtaagaaa agcaaagatt gctattgtta cttctggtgg tattgtaccc aaaggaaatc
    10681 ctgaccgcat tgagtcatca tctgcttcaa aatacggcga atatgacatc gaaggggtaa
    10741 tggatcttac agaagaaacc tatgagactg cccacggtgg ctttgaccct gtttatgcta
    10801 atcttgatgc agacagagtc attcccgttg atgttcttcg tcagtatgaa aaagaaggca
    10861 aaattggaga acttcaccgc tatttttaca gcacggtagg aaatggtaca gcagttgcaa
    10921 gctccaaggc tttttccaaa gaaattgggg caaagctgat tgaagatgga gttgacgctg
    10981 ttatacttac ttctacttga ggcacctgta ctcgttgcgg tgcaacgatg gttaagggaa
    11041 tcgaagaatt tggtatccct gtagtacaca tgtgtacagt tgttcctatt tcactgactg
    11101 taggagccaa tagaattgtt cctactgtag ctattcccca tcccctgggc aatccaaatc
    11161 ttgacgctca ggaagagctg gctcttcgta agagacttgt tgataaggct ctagccgctc
    11221 tagaaacaga agtggaagag caaactgtct ttgaataatc ataagagggg tgatagagat
    11281 atcacccctt tttatcacaa attattgagg aggaattgat gttcccagta ttaaaagcag
    11341 ctagttatgt tcttatgcat gcacctgata tggtgcttaa aaatggtacg actcaaacaa
    11401 ccgaaagaat tctaaatcct gaaagtgagt atcttacaaa ggttaatgag aacttacgcc
    11461 cttttgaaga gattgtaagt tatcctgcta accagtgcta tattggaaat attacacccg
    11521 aagacttagc tacttatgag atgccttggt atgataaggc agttgaagga gcgaaggccc
    11581 agggtaaaaa gggtgagatc tttgcagagg atgtttttat cgccctaata aaagcttctg
    11641 atgcctttga tttggttctt ctggaaaaag actttacaga ggctgttcgg cctaaactgg
    11701 acgcttatac aggactaaat gcaaaagcaa aaactcttcc taaaggggca gaactagaaa
    11761 gaattcaaac tgaaattgaa aaacgagatg ctgaacccct tcttcataaa ggagaggtgg
    11821 taggttgtgt taaaaaggcc catgatattg atattaacct aaatgctcat gttattcttg
    11881 agaatctggt agtaaaagca tctgggacca tggcaatgga aaggcttttt gtgcttaaca
    11941 atatcgatcc agccacagtt gattatgtga ttgagtgctc tgaagaagcc tgtggagata
    12001 tgaaccagcg cggtggcggg aactttgcca aggccctggc tgagatgacc gactgtctta
    12061 atgccacagg tagtgacact cgaggctttt gtgcagcacc aacccatgcc ttaattgaag
    12121 cagcagcctt tgttaaatct ggtgtcttta aaaatgtcgt tgtagttgca ggtggctcca
    12181 cggccaaact tggtatgaat gggaaagatc atgttaaaaa aggaatgcct atattagaag
    12241 atgttgttgc tggatttgca cttcttgtat ctgagaacga tggccagcat ccaattctaa
    12301 gaacagatct agtagggggg catactgttg gtacaggatc ttctcctcaa aacgttatga
    12361 gtgctctaat taccaaggcc cttgataagg gtgatttgaa aatcactgat attgataaat
    12421 actctgtaga aatgcagaac cctgatatta caaaaccagc tggagcaggg gatgttcccc
    12481 aggccaacta taagatgatt gcggctttag gagttatgcg taaggatcta gagcgcagcc
    12541 agcttaatag ttttgttcaa acacatggta tggtcggctg ggccccaacc cagggacata
    12601 ttccttcagg tgttccctat tgtggatttg caatagatga tattatgagc gaaaaaatta
    12661 aacgggctat gattgtagga aaaggatctc tcttccttgg ccggatgaca aatctttttg
    12721 acggtgtatc tattgtggta gaaaagaatc ctggtatcca agaggaagaa ggaagtgctt
    12781 ccaaagagga aatcagaaca atggttgccc aagccatgag agactttgca gcttccctga
    12841 atcaatagga ggaaacatgg aaaatctaaa ggtaaaagca atcatcgcag atgtatttga
    12901 ggagattgct cagggcttgg aaacaggcct tttccacgat aaagttgctc ttggtcttac
    12961 tctttatgga agcgagcttg gtgcctctaa tcttcttaaa ggcgcagaaa catttgctaa
    13021 agacaatgcc gacgtggaag tggttctaat tggacctaaa gttgaaacag atctgactct
    13081 tattgaagtc gaaagtgaag aacttcagta tgataaaatg gaagaacttc ttgatagtgg
    13141 tagaatccaa ggctgtgtga cgatgcacta tagcttccct atcggtgtat ctaccgtagg
    13201 taggactctt accccaggtt ttggtaaaga gatgtatgta gccactacta caggcactag
    13261 ctcggcccac cgcaatgagg ccatggtaaa aaatgccatt tcaggtatta ttgcagccaa
    13321 ggccgcaggc attcaagaac ctagcctagg tattcttaat gttgatggag ctcgaacagt
    13381 agagcgtgcc ctgaaagaac tttctgctgg aggctaccct attaactttg ctgatagtga
    13441 tcgatcagat ggtggatgtg taatgcgagg aaatgatctt cttcgtggaa cacctgatat
    13501 catggtcact gacaccctta caggaaacat cctgatgaag actttctctt cttatcatac
    13561 gggagggaat ttcgaggctg tgggagatgg ctatggtcct ggtattggcc aagactttga
    13621 tcgccttgtt cttattcttt caagagtaag tggaagccct gttgtggcaa atgccctagc
    13681 ttatgctaag gatcttgtcc taggggatgt ccataagcta agcaagaaag aatacaagaa
    13741 ggccaaggat gcgggtctgg aagaaatatt agctgggctt acaaagacca aagaaaaagc
    13801 tcaggaagaa gaagttaccg ctcctgctga agaaataacc acaggagaaa taagcggaat
    13861 tgatattctt gaactagaag atgctgtaag agttctgtgg aaagaaggca tatacgctag
    13921 ctctggtatg ggctgtacag gcccagttgt tatggtcaat gaatcaaaaa tcggagttgc
    13981 cagtcaaatt ttagcagctg ccggctatgt atctgaagac taataagtaa gtaaaaaaga
    14041 gcccttgcct tatagataga atctataaag gtgagtggct cttttttatt gcccttatag
    14101 ggaaaaaact ggactgctaa ttacaggaat tttcctgcta gggtctatag tagaagaaga
    14161 gactgcaaag gtgaaagtgc atatacaagt ctgtaaagcc ttgaaactaa agggatataa
    14221 gaagtcataa taatgttagg ccctgcttat atagtagtgg cagcttccat ctttttttac
    14281 atcaagatag agtattttag gctcatcatt ggactgattg taaaaatagg gagcagagtt
    14341 aaagaactca tcttcttcta tatgtctttt taagacactg gagtggtgga tgaacttttt
    14401 aaaatcatcc ctagaggcaa ggataggcat gcacataaaa caataaattc catcttcatc
    14461 ctcttcatag agattatagg gatcttcaga ggtaagggac ataagtttct tacaacttgt
    14521 tttaaccgcc ttttgatatt tggcttcaat tgtaacccct tcaagatgaa gggcagtttg
    14581 ataagcctgc caaggaatgt cctttggaat ctcagtgact ctttctgctt caatctttcc
    14641 ccgcaggagg gtttcgccct tatatccata tagggccata tcccccacat cctcgagatt
    14701 atcatcagaa aagtgataaa aaacgtaacg cccagagtca aaaaaatcat cagggcacct
    14761 tgctagaaag tgggagaggg taaatatatc aagatctata tgagagctat aatgccccca
    14821 gatcaggcca taatctgcaa taatttagat ctgactagca agggtcaaat ctcgcaaggc
    14881 atcttgcaac ttacttgtta tatttgaatc ggtaagggtc acttcctaat catcatattt
    14941 gatatagagc ttattaacag ttgcctgata caagatgttt ttcatagcaa gtagggaaaa
    15001 atggctctgg gctaagagtt ttatttgatc atcttcaggg tttccttccg gctttttatt
    15061 tatggttaaa tgttcaaaga cttgataata cac
//
LOCUS       DASRNA010000118         4697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_934838,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000118 DASRNA010000000
VERSION     DASRNA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934838"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="murB"
                     /locus_tag="VFD08_03165"
     CDS             <1..849
                     /gene="murB"
                     /locus_tag="VFD08_03165"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZK10050.1"
                     /translation="LLNKHCSFKIGGPGRLLIFPKNMEEIQYLVRILEGKDFLVLGNG
                     SNVLFRDQGYSGVIIKLAGNFSSFHIDENRVSASSGVSLSSLSRKVALKGLSGLEFAT
                     GIPGTLGGGVIMNAGAYEGELKDVVTRVTLMDKKGNIFEKTGEEMEFNYRNSLAQREG
                     YIVLKVELQLEEGNYKSIWEKIDDLSIRRWRRQPLEFPSGGSTFKRPKGYYAGKLIED
                     AGLKGLRFRGAQVSDKHSGFIVNSDGASCDDIRTLIRLVQKRVKDDSGVELEAELRLI
                     NDPNKG"
     gene            830..1564
                     /locus_tag="VFD08_03170"
     CDS             830..1564
                     /locus_tag="VFD08_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZK10051.1"
                     /translation="MIRIKADYHTHTHYSDGKGTVLDNVRAAHKKGLGTLAISDHSWG
                     HGIFGLKREQKNQYFKDIEEAGKIFPDMKILKGIECNILGPDGAIDLTPEEMEEFDLV
                     LCGYHFGSRIRSYKDLCLHGANALHKFTGLGEKRTRKVNTRALLRAMDYPIDVLTHPG
                     DKGPVDIVAVAEKAALKGIKLEINQRHKHLSLEQLNQIKDLDLEYILSSDAHRPEDIG
                     EVEKAYGNIIASGLDPKKIVNLKEVL"
     gene            1561..2403
                     /gene="rapZ"
                     /locus_tag="VFD08_03175"
     CDS             1561..2403
                     /gene="rapZ"
                     /locus_tag="VFD08_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZK10052.1"
                     /translation="MTIKIITGMSGGGKSTVLDSFEDMNYYCIDNLPPNLLGEFIRLY
                     ERMEENRKNMAVCMDLRLGVFFDEVPHTLDRLREQGYDVEIIFVEASDRALINRFREV
                     RRSHPMEKAGKTAEAICLERRRLAAIKEKSDIVIDTTKLTTYQLKKKLALLYGGKQDF
                     QVIIESFGMKNGMLENADFIFDLRFLINPYYVEELKELTGHDQVVRDYIMKEAVSKET
                     LKRLLDFLDICVEQFKNTGRNSIHIGLGCTGGRHRSVTFSLLMQEHFQKLDYSVLRQD
                     REQL"
     gene            2400..3380
                     /locus_tag="VFD08_03180"
     CDS             2400..3380
                     /locus_tag="VFD08_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HZK10053.1"
                     /translation="MKIVIIGGGTGLSVLLQGLKKITKNLSAVVNMVDDGGSSGILRS
                     DLGMLPPGDLRSCILSMSNTTELLEEVLNYRFDKGSLSGQSLGNLIMAALSDLTGGID
                     NALGACADLFNITGKVYAVTLDNIDLVAELKDGTKVLGESKIPLAVIENGSPIHKVNL
                     YPERPRAYRKVIEVIEEADLILVGPGSLYTSILPALLVSGVKEAILRSSGKCVYVANV
                     MTQPGETDHMSVSDHVRAIYDHIEDAIFDGILVSSTPLSPQDKKAYEDQGASPLFCHR
                     EDIEYLDKMGVKIIEDQFTQIVQGYIRHDADKVSRALIDYVMGITFYVEA"
     gene            3430..4632
                     /locus_tag="VFD08_03185"
     CDS             3430..4632
                     /locus_tag="VFD08_03185"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HZK10054.1"
                     /translation="MKDIIERLKKEESFLWLQDPGKKRSPFNLEDLEEARRFMESFAP
                     FLREVFPETKPSQGRISSGLKELPKMQKSLAIEKGRLFAKLDNELPVAGSIKARGGFY
                     EVLYHARKLAEEAGFYQEDPLNILNHRDFYDDYEIEVASTGNLGLSIGIISATLGFRV
                     SVHMSSDAKQWKKDLLRKKGARVLEYPGDYTAAVKEARKASLSKGSYFVDDEKSPYLF
                     MGYALAAYELKEQLEEKKIRNQPLYIYLPCGVGGAPGGIAYGVSLLLPKAKIYFAEPT
                     KAPAVLLGMLTGKHEKISVADIGLSGKTDADGLAVGRASSLVGPLMVGRIDGIYTLDD
                     STMLAYLKTLYESENLKVEPSACAGFKGPSFIDKEGIHLVWLSGGSLVPEENFAEYLE
                     RAREDRDK"
BASE COUNT         1462 a          809 c         1154 g         1272 t
ORIGIN      
        1 ttattaaata aacactgtag ctttaagatc ggtggacctg gaagacttct aatttttcct
       61 aaaaatatgg aggaaattca atatttggtt aggatccttg aaggaaaaga ttttttggtt
      121 ttgggaaatg gaagtaatgt cctttttaga gaccaggggt atagtggggt aattattaag
      181 cttgcgggga atttttcctc ttttcatatt gatgagaata gagttagcgc atcttctggt
      241 gtatccctat cctcgctctc aagaaaagta gcccttaagg gcctaagtgg actggaattt
      301 gcaacgggga tccctggcac tttaggtggt ggcgtcatta tgaatgctgg cgcctatgag
      361 ggggaactta aggacgttgt aaccagagtt actcttatgg ataaaaaggg aaatatattt
      421 gaaaaaacgg gcgaggagat ggagtttaat tatcgaaact ccctagccca gcgtgagggt
      481 tatattgtac taaaagtaga gctccagtta gaagagggca actataaaag catttgggaa
      541 aaaattgatg atttgtctat ccgccgatgg agaaggcagc ccttggaatt tcccagtggg
      601 ggcagtactt ttaagcgtcc taagggatac tatgcaggaa agctaattga agatgctggc
      661 cttaagggtc tgcggtttcg tggtgcccag gtgagcgata agcacagcgg ctttattgtc
      721 aatagtgatg gagccagctg cgatgatata agaaccctga tacggctggt acaaaaaaga
      781 gtaaaagacg atagcggggt tgaactagaa gcagaattga ggctaataaa tgatccgaat
      841 aaaggctgat tatcatactc atactcacta ttccgatgga aagggcactg tacttgataa
      901 tgttagggca gcacataaaa aaggccttgg gactctagcc ataagtgatc attcctgggg
      961 gcatggtatt tttggactta aaagagagca aaaaaaccag tattttaagg atattgaaga
     1021 ggcagggaaa atatttcctg atatgaaaat ccttaaggga attgaatgca atatccttgg
     1081 acccgatgga gcgattgatc tgactccaga agaaatggaa gaatttgacc tggtcctatg
     1141 tggatatcat tttggcagcc gaattagaag ctataaggat ctttgcctgc acggggccaa
     1201 tgccctgcat aaatttactg gccttggtga aaaaaggacc agaaaagtca atacccgggc
     1261 acttttaagg gccatggact atccaataga tgtactgacc catcctggtg ataagggtcc
     1321 tgttgatatt gtagcagtgg cagaaaaagc ggccctaaaa ggaatcaagc ttgaaatcaa
     1381 ccagcgtcac aaacacttaa gcctagaaca actaaatcaa attaaggatc tggatctaga
     1441 gtatatttta agttccgatg cccatagacc cgaagatatt ggggaagtag aaaaagccta
     1501 tggaaatatt attgcctcag gccttgaccc caaaaaaatt gttaatttaa aggaggtctt
     1561 gtgacgatca aaataataac aggtatgtcc ggcggaggaa agagtacggt actcgatagt
     1621 tttgaagata tgaactacta ttgtattgat aatctacctc cgaatttatt aggagaattt
     1681 atccgtctat atgagcgcat ggaagaaaac agaaaaaaca tggctgtttg tatggatttg
     1741 cgtctggggg ttttttttga tgaggttccc cataccttag atcgcctaag agagcagggc
     1801 tatgatgtgg agattatatt tgtagaggca tcagaccgcg ctcttatcaa tcgttttaga
     1861 gaagtgcgcc gaagtcatcc catggaaaaa gctggaaaaa cggcggaagc catttgccta
     1921 gagcgcagac gactagcagc aattaaagaa aaatcagata tagtaatcga tacgaccaaa
     1981 ctaacgacct atcaacttaa aaagaagtta gcccttcttt atggcggcaa gcaagatttt
     2041 caggttatta ttgagtcctt tggaatgaaa aacggcatgt tggaaaatgc agattttatt
     2101 tttgatttgc gttttcttat aaatccctac tatgtcgagg aattaaaaga acttacgggt
     2161 cacgatcaag tcgttcggga ttatattatg aaagaagccg tttcaaagga aacgctaaaa
     2221 cgccttctag actttttaga tatttgtgta gagcagttta aaaatacagg aagaaatagc
     2281 atccatattg gacttggttg tacgggggga cggcaccggt cggtaacttt tagcctgctt
     2341 atgcaagaac atttccagaa gctggactat tctgttttac gccaagatcg tgaacagtta
     2401 tgaaaattgt aatcattggc ggtggtaccg gtctttcggt tcttctgcag ggtttaaaaa
     2461 aaatcacaaa gaacttaagc gctgtggtca atatggtgga tgatggggga agtagtggaa
     2521 ttcttcgctc agacctgggc atgcttccac caggagactt aagaagctgc atcctttcta
     2581 tgagcaatac cactgagcta ttagaagagg tccttaatta ccgttttgac aagggctccc
     2641 tttcagggca gtccctggga aatctcatta tggcagcact gagtgatctt acagggggca
     2701 ttgataatgc cctaggagcc tgtgccgatc tttttaatat aacaggaaaa gtatacgcag
     2761 taaccttaga caatattgac ttagtagctg agcttaagga tgggaccaag gttttgggtg
     2821 agtcaaaaat ccccttggca gtgattgaaa atggaagtcc tatccataaa gtcaatttat
     2881 atcccgagcg gcccagagcc tatagaaaag taattgaagt aattgaagag gcagacctga
     2941 ttctagtggg ccctgggagc ttgtatactt ctattcttcc ggcccttctt gtatctggag
     3001 tcaaggaggc tattttgcgt agcagtggca aatgtgttta tgtggccaat gtaatgaccc
     3061 agccaggaga aacagaccat atgagcgtat cagaccatgt gcgcgcaatt tatgatcata
     3121 tagaagatgc tatttttgat ggaatacttg ttagttctac tccccttagt ccccaggata
     3181 aaaaggccta tgaggatcag ggggcaagtc ctcttttttg tcatagggaa gatattgaat
     3241 acttggacaa aatgggagta aagataatag aagatcagtt tacgcagatt gttcagggct
     3301 atatacgcca tgatgctgat aaggtaagtc gcgctttgat tgactatgtt atggggataa
     3361 ccttttatgt agaggcataa aaccccttga aaaaagacac aaataaagac tttagaagag
     3421 gccaagtaaa tgaaagatat tattgagaga ttaaaaaaag aagaatcctt tctttggcta
     3481 caggatccag gtaaaaaacg ctctcccttt aatctggaag atctagagga ggcaaggcgc
     3541 tttatggaaa gttttgctcc ttttttaagg gaggtatttc ctgaaaccaa accaagccag
     3601 ggaagaatta gctcgggatt aaaagaactg ccaaagatgc aaaaatcttt ggcaatagaa
     3661 aaaggaaggc tatttgccaa gttagataat gagctgccgg tggcaggaag tataaaggcc
     3721 cgtgggggtt tttatgaagt tctttatcat gcaagaaaac tagcagaaga agcaggcttt
     3781 tatcaggagg atcctctgaa cattcttaac caccgggatt tttatgatga ttatgagatt
     3841 gaagtggcca gtacgggaaa tctaggactt agtattggca ttatcagtgc cacccttggt
     3901 tttagagtaa gtgtccatat gtctagcgat gctaagcagt ggaaaaaaga cctgctaaga
     3961 aaaaaaggag ccagggtcct tgagtaccct ggtgattata cggctgctgt taaggaagca
     4021 agaaaagcct ctttaagcaa ggggtcctac tttgtggacg atgaaaaaag cccctatctc
     4081 tttatgggct atgcactggc agcctatgaa ttaaaagagc aactagagga aaagaaaata
     4141 agaaatcagc ctctttatat ttatcttcct tgtggggtgg gaggcgctcc tggtggaatt
     4201 gcttatgggg taagcctgct tcttcctaaa gcaaaaattt attttgctga accgactaaa
     4261 gcgccagcag tgcttttagg tatgcttaca ggaaaacatg aaaaaatcag cgtggcagat
     4321 attggactta gtggcaagac cgatgccgat ggcctggctg taggtagggc ttcttctctt
     4381 gttggccccc taatggtagg aagaattgat ggaatatata ccctagatga ctccactatg
     4441 ctagcctatc ttaagactct ttatgaaagt gagaatctta aggtggaacc ttcagcctgt
     4501 gcaggtttta aggggcctag ctttattgat aaagaaggaa ttcatctagt ttggctaagc
     4561 ggtggcagcc ttgtgccgga agaaaatttt gcggaatatc tagaaagagc cagggaagat
     4621 agggataaat aaatctagct tataagcaag agggtggaag gaagttcccc ctttttttaa
     4681 aatgataaca aagagcc
//
LOCUS       DASRNA010000119        11049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_934884,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000119 DASRNA010000000
VERSION     DASRNA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934884"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VFD08_03190"
     CDS             <1..572
                     /locus_tag="VFD08_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZK10055.1"
                     /translation="VERVYKKFGRCTIAVSEGIEDEKGNPVISQLVEDEVDSHGNVQL
                     SGNGLLGDMLAEAIKKRLNISRVRADTFGYLQRSFLGVRSDVDAKEAREVGEVGAKLA
                     FKGLSGSVVMRRIPPYRVYYELVPIEKVARYSKSMPREFMNKEGNFVSEEFIQYARPL
                     VGNDIPEIAVLRSPPIPKTFLVKHGEDVE"
     gene            complement(636..1697)
                     /locus_tag="VFD08_03195"
     CDS             complement(636..1697)
                     /locus_tag="VFD08_03195"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZK10056.1"
                     /translation="MKIFDLLQEHDYEQLVFCQDKQTGLKAIICIHDTTLGPGLGGTR
                     IWDYETEDEAIVDVIRLARGMTLKNSAANLNLGGGKAVILGDPEKIKSEALFRTFGRY
                     IEGLSGRYITAEDMNSTTQDMAWVNDETDYVVGLEGKSGDPSPVTGFGVFRGIQAACN
                     EVYGSDDLKGKVVAIQGAGAVGYTLCEHLHEAGAKIIVTDIRKESIDRIVNDFGASPV
                     EPDEIYGVDCDIFAPCAMGAIINDFTVDKLKCKIVAGSANNQLAEDKHGKMLEDKGIL
                     YVPDFVINGGGVINVFEELKGYNEKRALARASAIYDTCKTIFAIAKDEGITTHEAAER
                     TAINRIESMAKVNSLYLDR"
     gene            1945..3897
                     /locus_tag="VFD08_03200"
     CDS             1945..3897
                     /locus_tag="VFD08_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZK10057.1"
                     /translation="MYFGYFEVVRSERGILVYLFLSTLFMEIILRWQSGQAFFGQGLF
                     FILWFVLAFVLLSMAVLHLVPRHIGRILIRIWFVLVTLIFVVQFVYFSIFKTYLTFYS
                     MMHGAQAFSFYKVIIIAMLRDLRWILLFLPLLLFVFIKKEFTWVPRGGFVDYGLLFVL
                     NILLLATLIGMTEHSDLSRKNILASSIEPVVGIHKVGVIGNGYGEVHRRIFTQKKPYY
                     APKKAIVPRPGENVAYNLGGFLELEDDPLLSEMTSYFLSRQATPKNTFTGRLAGYNLI
                     YIMAEGFSDWVVDEDLTPTLYRLMQEGVQFQNFYNPLWGVSTTDGEYSALTGLFPVPG
                     IWSMVESKDKALPQTLGNQLSEMGYHSDAFHNHDHTYYQRNKTHSNLGYNFYALGAGL
                     ELEKIWPASDGEMIEKSLARYIDKDPFHVYYLTVSGHMPYDFSNNKMSKRHQDKVEHL
                     PLSEEAKAYIACHIELDQGVETLVQALEERGILDKTLIVLHGDHYPYGLKKTTIDELA
                     GSELEGPELYRSKIIFYSPSLKGVRVNRLSTTLDLLPTLSNLFGIQYDSRLMMGRDLF
                     SYSSPLVEFADGSFITPQGYYNSMRNKSDGFSQEELAALKMKVEKKFYYSKKMLETDY
                     FRQIEPYRLKSLPPTGVLDLTEEEKE"
     gene            4048..4587
                     /locus_tag="VFD08_03205"
     CDS             4048..4587
                     /locus_tag="VFD08_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZK10058.1"
                     /translation="MQIGERLKDLRHKNGLTQEELADRSDLTKGFISQIENANTSPSV
                     DTMESLVRALGTNMSDFFKEDQPIHVVYYNEDAFSAFYEKLGSTISWIVPDAQKNHME
                     PVLLELAAGGKSKTYTPFEGEAFGYVLEGEIDLHYGDEDYQVKSGDCFYFEANRPHSI
                     SNPSKEKAKVLWVLTPPNF"
     gene            4598..5656
                     /locus_tag="VFD08_03210"
     CDS             4598..5656
                     /locus_tag="VFD08_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10059.1"
                     /translation="MATQIELRNIEKSFGDHRVLDQINLEIEEDEFLTILGPSGCGKT
                     TILRLIGGFEIPDGGEVIFDGKKITKVPAYLRPVNTLFQKYSLFPHLNVYDNVAFGLR
                     IKKRPEAEIASRVGEMLGLVNLKGFEKRGVESLSGGQQQRIAMARALINNPEVLLLDE
                     PLGALDLKLRKEMQVELKRIQRELGITFIFVTHDQEEALTLSDRIVVLNEGVIQQIGT
                     PVEVYNEPINSFVADFIGESNILEGIMHKDYDVSFSGRRFVCVDRDFYPNEAVDVVIR
                     PEDVELVPRDKALLLGEVLSCVFMGVHYEMQIATPEEKYLVHSTLAREIGETVGLYVE
                     ADNIHIMRKGEVEGDEEL"
     gene            5643..6482
                     /locus_tag="VFD08_03215"
     CDS             5643..6482
                     /locus_tag="VFD08_03215"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10060.1"
                     /translation="MKSFKKAAAPYFIWALLFIIVSLLMVLYYAFTGTSGSFSLENFQ
                     RFFAGTSLGTLGRSLKIALITTVICFILGYPLAYSISKLKLAHRSLAVMLIMLPMWMN
                     FLLRTYAWVTILSKNGVINSLLRYFGAGQLDLLYREGAVILGMVYNFLPFMILPIYTV
                     LEKMDEGLLEAAADLGCNKLMTFIKVTFPLSLAGVVSGISMVFIPAISTFEITTLLGG
                     NKTNLIGNVIEQQFTVTGDWNYGSAMSVVLMMVLLLSLFFTGGEEKMLGQSIRSGKEV
                     GHG"
     gene            6475..7242
                     /locus_tag="VFD08_03220"
     CDS             6475..7242
                     /locus_tag="VFD08_03220"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10061.1"
                     /translation="MAKFIRRFYMFLMYGFLYAPILVLIVFSFNEGRTRGSWDGFSLI
                     WYKKLFMDREILMAVYHTFLVASIATLVSTVIGVLGAIGISPYRGKLKSAILGMNQIP
                     VLNPDIIIAVGLMVFYRSLGLSSGLLTLTLSHIAFTVPYVVLSVLPKLKQMPPSLAEA
                     AMDLGASPAQTLLKVILPYIKPGIISGALLAFTLSVDDFVISFFTTGNGVETVSTLVF
                     SMARRGINPEINALSTIMFAIVLFLLILMNLRTEKTS"
     gene            7252..8289
                     /locus_tag="VFD08_03225"
     CDS             7252..8289
                     /locus_tag="VFD08_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK10062.1"
                     /translation="MKGKKIMVFFLLVLILTSCTIREDAQEVVNLYNWGDYIDKDLLR
                     KFEQETGIGIIYSNYNTNEDMYVKLKSGGTNYDVVIPSDYMIERLIKEDMLRKINWDN
                     IPNRKYLDPEYLFHSYDPQQEYSMPFFWGTLGIIYNRTLVDDVVDSWGILWDEKYHRE
                     IIMLDSSRDSLGIALKYLGYSMNSLDIDQLEEARDLLAQQYPLVYAYLVDQTRDIMIN
                     GEAALAVIYSGDAMDAISNNEDLDYIIPKEGSNLWFDAMVIPATAKNPENAEAFINFM
                     LEPENAAQNSEYIYYSIPSSEARALLDEELRDSEVAYPPKEILSGLEVFHDPGAYIKI
                     YDDLWQDVKNQ"
     gene            8345..8965
                     /locus_tag="VFD08_03230"
     CDS             8345..8965
                     /locus_tag="VFD08_03230"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HZK10063.1"
                     /translation="MRNKIPAFFFSLALILGVVLVLASLVPVYDLLQGLSGVEETSGY
                     NKNFLHKANTAIRDYLYGYRDNLLIESDGKSLFGGQEIFHMAEVRLLFTRLARAGLLF
                     FALALLSSYLLKTRLLYHQLFTSLGLLVFMALAGAFFEKAFILMHRVFFNNDLWLFPP
                     DSQLIVLLPESFFYYFTLLIIFVFIGMSVIFYLIERIYHDFTSGPR"
     gene            8940..>11049
                     /locus_tag="VFD08_03235"
     CDS             8940..>11049
                     /locus_tag="VFD08_03235"
                     /inference="COORDINATES: protein motif:HMM:NF045064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10064.1"
                     /translation="MILHLGRDSKEKRREIYKKILSAPSASIITPEQATLDLEKDIIE
                     TLGLPGLLFHEVTSFSRLLHRMIERYEGEEVLSDVGKYLLLRQVVEESKDKLPLYGKS
                     FDKDGFYEELSSAIELFQRTGISGEEILKLEESKRLKELGLIYRAYENTLQEQGFYQD
                     AALELFESSPALGLYSSDQVWILGYKALDYMQWKMIDAINPHTQIHLVLSYEEDDIFM
                     PTRRTKEALERNYLCSHHYYKSPKSERVAFSKELVYDEGKKRDFVPEVFSAKDPYSEA
                     EFVGLHILKRMKEDPTLSYGDFKILVSDMDSYDFVFRQIFTALGLPLFSDRRRSILES
                     PMIQTFLSLLRIYQRGFKREDLLGFLKGYVPERDWEDLDLFENYCFSRGIGGKRFKES
                     FPDKAMEELRIKFLADLIYFEDSFKKKASPQDFGELLIELLHRLDFAKRLEEEAEFYA
                     KKAEGEQSQLLSQLWQSLLELISQISAVGPKKELSFVAYCKLIHVGLSNMSLGVIPPA
                     VDRIRLATLYRSTHDPVDYLYFCGMNDGLLPREYSEALLLKEEDKRLLQEGGFPLYDN
                     FEHKEELDRLDLFTALSLVKKEYFFSYALADIQGKSKLPSLFIERILERTGAQFYSSA
                     HSDKYLQDYYYLHPSMSFRYGLKALRENREETIYGLSQEKIRQMRQRLEEAPLVPLLK
                     RRDKRLHLSISRLERFRRCPF"
BASE COUNT         3138 a         2137 c         2433 g         3341 t
ORIGIN      
        1 atgtagaaag agtttataaa aaatttgggc gctgcacaat tgccgtgagc gaaggcatag
       61 aagacgaaaa aggaaatcct gtcatctctc aacttgtgga ggatgaagtg gattcccacg
      121 gaaatgtcca gctctcggga aatggccttt taggggatat gttggccgag gccatcaaaa
      181 aaaggctcaa catttctagg gttagggcag atacctttgg ctatcttcaa cgttcttttt
      241 taggtgtacg cagtgatgtt gatgcaaaag aagcaagaga ggtgggtgaa gtaggagcaa
      301 aacttgcctt taaaggtctt tctggttctg ttgtgatgcg ccggattccc ccctatagag
      361 tctactatga gctggtacct attgaaaaag tagcccgtta tagcaaatct atgcctagag
      421 aatttatgaa taaagaagga aattttgtta gtgaagaatt tatccaatac gcaagaccac
      481 ttgttggaaa tgatatacct gagatagcag tccttagaag cccccccatt ccaaaaacct
      541 ttttagtcaa acatggcgaa gatgtagaat aatagactag ttatagaaaa aacaaggctc
      601 ccggcaggga gccttgtctg tttatcggtc tctatttatc ggtctaagta aagtgaatta
      661 accttggcca ttgattctat acggtttata gcagtacgct ctgcagcttc atgggtggta
      721 ataccctcat ccttagcaat agcaaagatt gttttgcatg tatcgtagat tgcactggct
      781 ctagcaaggg cacgtttttc attgtatcct ttgagttctt caaagacatt gattacgccg
      841 cccccattaa tgacaaagtc aggaacataa agaattcctt tatcctctaa cattttgcca
      901 tgcttgtctt ctgccaattg gttattggca ctaccagcaa caattttgca tttgagctta
      961 tcaacagtaa agtcgttaat gattgccccc atggcacagg gtgcgaagat gtcgcagtct
     1021 acgccgtaga tttcatctgg ttctactgga ctagctccaa agtcatttac aattcgatct
     1081 atgctttctt tgcgtatatc agtaacgata atttttgctc cagcctcgtg gagatgctca
     1141 catagggtgt agcctacagc accggcgcct tgaatggcaa caaccttgcc ttttagatca
     1201 tcacttccat atacttcatt acaggctgcc tgaatgcctc taaagacacc aaagcctgtt
     1261 acaggggaag ggtcgccact ttttccttct aggccaacaa cataatctgt ttcatcattt
     1321 acccaggcca tatcctgagt ggtactattc atatcttctg ctgttatgta acgtccacta
     1381 agcccttcaa tgtatctacc aaaggttcta aataaggctt cactcttgat tttttctggg
     1441 tctcctaaaa taacggcttt tcctccacca agatttaaat tggcagcaga gttctttagt
     1501 gtcatgccac gtgcaagacg aattacatcg acaatggcct catcttctgt ttcataatcc
     1561 caaattctag tcccgcctaa accgggtcct agggtagtat catgaataca gattatggcc
     1621 ttaaggcctg tttgcttgtc ctggcaaaaa acgagttgtt cgtagtcatg ttcctgtagt
     1681 agatcaaaaa ttttcatgaa aactcctttc ctttagaaga taccaaaacg aatgtatcca
     1741 tatatattat agcagtccta gaggcagata caagatggca gggagactaa aaacggcttt
     1801 ttgtttagaa atcaggctta tatagggcta aaaagagcaa aacttgccat ttatactact
     1861 ttgttgtaaa atagcatgac tattgaacaa agaagacttt ataaaacttc atgggatatt
     1921 atcccttgtt ctaaaggagg aactatgtac tttggttact ttgaagtggt gcggagtgaa
     1981 agagggatcc tagtctatct tttcttaagc acgctcttca tggaaatcat tcttagatgg
     2041 caatcgggac aggcattttt tggacaagga cttttcttta ttctttggtt tgtgcttgcc
     2101 tttgtacttc ttagtatggc agttttgcat cttgtgccgc gacatatagg ccgtatccta
     2161 attcggattt ggtttgtttt ggttaccctt atatttgtgg tacaatttgt ctatttttca
     2221 attttcaaaa cctatttaac cttttattct atgatgcatg gagcccaagc tttttctttc
     2281 tataaagtga ttatcattgc tatgcttcgt gatctgagat ggattctctt gtttttaccc
     2341 ctattattgt ttgtttttat caaaaaagaa tttacctggg tgccccgtgg tggttttgtg
     2401 gactacggtc ttctttttgt gctcaatatt ttgcttcttg caaccttaat agggatgaca
     2461 gagcattctg atttatcaag aaaaaacata ctcgcttcaa gcatagagcc agtggtgggg
     2521 attcataagg tgggagtgat aggaaatggc tacggggaag tgcaccggag gatttttacg
     2581 caaaaaaaac cctactatgc tcccaaaaaa gcaatcgttc cacggcccgg agagaatgta
     2641 gcctacaatt taggaggatt tcttgaatta gaagatgatc ctctcctatc agaaatgaca
     2701 tcctattttc taagcaggca ggctacacct aaaaacacct ttacaggtcg cctggcaggc
     2761 tataacttga tttatattat ggccgaaggt tttagtgatt gggtggtaga tgaggactta
     2821 acacccaccc tatatcgttt aatgcaagaa ggggtacaat tccaaaactt ttacaatccc
     2881 ctctgggggg ttagtacaac cgatggagaa tattctgccc taacaggact ttttcctgtt
     2941 ccaggcattt ggagtatggt ggaatcaaaa gacaaggccc tgccccagac cctgggcaac
     3001 cagctgagtg aaatgggata ccatagcgac gcctttcata atcatgacca tacctattat
     3061 caaagaaata agacccatag caatttgggc tataattttt atgctttagg ggcaggcttg
     3121 gagctggaaa aaatttggcc tgcaagcgac ggggaaatga tagaaaaaag ccttgctcgt
     3181 tatattgata aagacccctt ccacgtctat tatttgacag taagtggcca tatgccctat
     3241 gacttttcca ataataaaat gagtaaaaga caccaagaca aggtggaaca ccttcccctt
     3301 tctgaagagg ccaaggccta tattgcctgc catattgaac tagatcaagg ggtggagacc
     3361 ctagtgcaag ctttagaaga aaggggtata ctcgataaga ccttaattgt tctccatgga
     3421 gaccactatc cctatggctt gaaaaaaacc accattgatg agctggctgg aagtgaactt
     3481 gaagggccag aattgtatcg gagtaagatt attttttata gtccctctct taaaggggtt
     3541 agggtaaacc gtctttctac gaccctagat ctacttccaa ctctttcaaa tctatttggg
     3601 attcagtatg attcaagact tatgatggga agggatcttt ttagctatag ttctcccctg
     3661 gtggaatttg ctgatggaag ctttataacg ccccagggct actataatag catgagaaat
     3721 aagagcgatg gatttagcca ggaggaactg gcagccttaa aaatgaaggt ggaaaaaaag
     3781 ttctactact caaagaagat gttagaaaca gattatttta gacagattga gccctataga
     3841 ctgaaaagcc ttccccccac tggggtgctg gatttgacag aggaagaaaa agagtagggt
     3901 tttataagaa aaaaattaaa tagaagaagg aaaaaaggca tggcgccaag ctatgccttt
     3961 ttttaaaaga aaagacttgc ctttggataa atagtggtat acaatactta aagtttagta
     4021 taccacacct ttagaaaaga ggaagagatg caaataggag aaagacttaa agatttacgc
     4081 cataaaaacg gactgaccca ggaggagcta gccgatagat cagacctgac taagggcttt
     4141 atatcacaaa ttgaaaacgc aaatacaagc ccttcagtag ataccatgga gtcccttgtt
     4201 agggccttag gaacaaatat gtccgacttt tttaaagaag atcaacctat acatgttgtt
     4261 tattataatg aagatgcctt tagcgccttt tatgaaaaac taggctctac catttcttgg
     4321 attgttcctg atgcgcaaaa gaaccatatg gagccggttt tacttgagct tgcagcaggg
     4381 ggtaaaagca agacctatac tccctttgaa ggagaggcct ttggttatgt tcttgaaggg
     4441 gagattgact tgcactatgg cgatgaggat tatcaagtca agtcagggga ctgcttttat
     4501 tttgaggcaa accgtcccca tagcattagt aatccctcaa aagaaaaggc aaaagtactt
     4561 tgggttttaa ccccaccaaa cttttagagg agaaactatg gcaacacaaa tagaactaag
     4621 aaatatcgaa aagtcctttg gtgatcatag ggtccttgat caaatcaatc tagagataga
     4681 agaagatgag tttttgacca ttctgggacc ctctggctgt ggcaagacaa ccattttgag
     4741 gttaattgga ggctttgaaa tcccagatgg gggtgaagtt atctttgatg ggaaaaagat
     4801 tacaaaagtc ccagcctacc tgcgacccgt aaatactctt tttcaaaagt actctctttt
     4861 tcctcacctt aatgtctatg ataatgtagc ctttggacta cgcataaaaa aaagacccga
     4921 agcagagata gcctctcggg taggagaaat gcttggccta gtaaatctta agggatttga
     4981 aaaaaggggg gtggaatccc tctctggggg tcaacagcaa cgcatagcca tggcccgggc
     5041 cctaattaac aatcccgaag tattactttt agatgaaccc ctaggagccc tggatcttaa
     5101 acttcgaaaa gaaatgcagg tggagcttaa gcgtatccaa agagagcttg gtattacctt
     5161 tatatttgtt acccatgacc aggaagaagc ccttactctg agtgatagaa ttgttgttct
     5221 aaatgagggc gtcatccaac aaattggtac tccagtagag gtctacaatg aacccatcaa
     5281 ctcctttgta gcggacttta ttggtgagtc caatatccta gaagggatta tgcacaaaga
     5341 ctatgatgta agcttttctg gacgccgctt tgtttgtgtg gacagggact tttatcccaa
     5401 tgaagcagta gatgtagtca ttcgcccaga agatgtggaa ctcgttccaa gggataaggc
     5461 cctgcttttg ggagaagttt tgtcttgtgt ttttatgggg gtgcactatg agatgcaaat
     5521 tgccacccca gaagaaaagt atctagttca ctccaccctt gctcgagaaa ttggagagac
     5581 ggtgggccta tatgtggaag cagataatat ccatattatg agaaagggtg aggtggaggg
     5641 tgatgaagag ctttaaaaaa gccgcagccc cgtattttat atgggccctg ctctttatta
     5701 tagtgtccct tttaatggtc ttatattatg cctttactgg tactagtgga agtttttccc
     5761 tggagaactt ccagcggttt tttgccggta ctagcctagg cactctgggg aggtctctaa
     5821 agatagccct tattacaaca gttatttgct ttatcctggg ctatcccctg gcctatagta
     5881 ttagcaagtt aaaacttgcc cataggagcc tggctgtaat gctaattatg ctgcctatgt
     5941 ggatgaactt tctgctgcgc acctatgcct gggtaaccat cctttcaaaa aatggggtga
     6001 taaatagtct gctgcgctat tttggggcag gccagctgga ccttctttat agagagggtg
     6061 cggtaattct gggtatggtg tataactttt tgccctttat gattctaccc atctataccg
     6121 ttcttgagaa aatggatgag ggtcttctag aggcggcggc tgatcttggc tgcaataagc
     6181 tgatgacctt tattaaagtt acctttcccc tttctcttgc gggtgtggtc tctggtatat
     6241 ctatggtctt tatcccggct atctcaacct ttgagattac cacccttctt gggggcaata
     6301 agacaaatct tattggtaat gtgattgagc agcagtttac tgtaacaggg gactggaact
     6361 atggctctgc tatgagtgta gtcctaatga tggtgctgct tttatccctt ttctttacag
     6421 gaggagaaga gaaaatgcta ggccagtcaa ttcgttctgg caaggaggtg ggccatggct
     6481 aaatttatac gacgttttta tatgttttta atgtatggtt ttttatatgc cccaattctt
     6541 gtacttattg tcttttcctt taatgaagga agaacaaggg gaagctggga tggtttttct
     6601 cttatctggt ataaaaaatt atttatggat agagaaattc ttatggctgt ctaccatact
     6661 tttttggttg ctagtattgc tacccttgtt tctacggtaa taggagtctt gggagccatt
     6721 gggattagcc cctatcgtgg caaacttaaa agtgccattt tgggaatgaa tcagataccg
     6781 gtcctaaatc ccgatattat cattgcagtg gggctgatgg ttttttatcg gagcctgggt
     6841 ttaagttcag gtcttttaac cctgactctt tctcatattg cctttaccgt cccctatgtg
     6901 gttttgtcgg tcctgccaaa actcaagcag atgccccctt ccttggcaga agcagccatg
     6961 gacttgggcg ctagtccagc ccagactctg ctaaaggtaa ttcttcctta tattaagccg
     7021 ggtattatct caggagccct gctggctttt actctttcag tggatgattt tgtgattagc
     7081 ttttttacca caggaaatgg ggttgaaacc gtatcaacct tggtcttttc tatggcaaga
     7141 agagggataa atcctgaaat caatgcactc tctacgatta tgtttgccat tgttttattt
     7201 ctcttgattc ttatgaatct tcgcacagaa aaaacaagct agggggaaaa gatgaaagga
     7261 aaaaagatta tggtgttttt tcttctagtg cttattttaa ctagctgtac tataagagaa
     7321 gacgcccaag aggtggtaaa cctctataac tggggagact atattgataa ggatctacta
     7381 agaaagtttg agcaagaaac aggaataggg attatttatt ccaactacaa taccaatgaa
     7441 gatatgtatg taaagttaaa aagtggtgga acaaattatg atgtggtaat cccttcagat
     7501 tatatgattg aacgattgat aaaagaagat atgcttagga agataaattg ggacaatata
     7561 ccaaatcgaa aataccttga tcctgagtat ctttttcata gctatgaccc ccagcaggag
     7621 tattccatgc cttttttctg gggaaccctg ggaattatct acaatagaac ccttgtggat
     7681 gatgtggtgg actcttgggg gatactgtgg gatgagaaat atcatagaga aatcataatg
     7741 ctggattctt cccgagactc tcttggtatt gcccttaaat atctgggtta ttctatgaac
     7801 tctctagata tagaccagct ggaggaggcc agagaccttc ttgcccagca gtatcccttg
     7861 gtctacgctt atttagtgga ccagacaagg gatataatga tcaatggcga agcagccttg
     7921 gcagttattt attcgggaga tgccatggac gctatatcca ataatgagga cctagactat
     7981 attattccaa aagaagggtc taatctttgg tttgatgcca tggttattcc tgccacagca
     8041 aaaaacccag aaaatgcaga agcctttatt aattttatgc tagagcctga aaatgcggct
     8101 cagaattcag aatatatata ttactctatc cccagctctg aggccagggc ccttcttgat
     8161 gaagagctaa gagattcaga agtagcctat cctcccaaag agattttatc cggcctagaa
     8221 gtctttcatg atcctggtgc ctatatcaaa atatatgatg atttatggca ggatgtaaag
     8281 aatcaataac cccctttttt gggggttttt ccttgtccta tgctatacta tttgtgaggt
     8341 gaatatgcga aataaaattc ctgctttctt ttttagtctg gccctaatcc ttggggtggt
     8401 gctggtttta gcctccctag taccggtcta tgatcttctt caagggctat cgggagtaga
     8461 agagacaagt ggctataata aaaatttttt acataaggca aatacagcta ttagagatta
     8521 cctatatggc tatagggaca atctgcttat tgaaagtgat ggcaagagcc tttttggtgg
     8581 ccaggaaatc tttcatatgg cagaagtaag gcttcttttt acccgccttg ccagggccgg
     8641 cctgctattt tttgctctgg ctcttttatc ttcttattta ttaaaaacaa ggctgcttta
     8701 ccatcagctt tttacctctt tgggactgct ggtttttatg gctttggccg gagccttttt
     8761 tgaaaaggcc tttattttga tgcaccgggt attttttaat aatgatttgt ggctttttcc
     8821 cccagatagc cagctgattg tccttcttcc tgagagcttc ttttattatt ttactttact
     8881 tattattttt gtttttattg gaatgagtgt tattttttat ttaatagaaa ggatctacca
     8941 tgattttaca tctgggccga gatagtaagg aaaaaagaag agaaatttat aagaaaattt
     9001 tgtccgctcc aagtgcttct attataaccc cagaacaggc cactctggat ttagaaaaag
     9061 atattataga gaccctgggg ctgccgggac ttctttttca tgaagtaact agtttttccc
     9121 gcctcttgca ccggatgata gagcgctatg agggcgagga agttcttagt gatgtgggta
     9181 agtatcttct gcttcgccag gttgtagagg agagtaagga taaacttcct ctttatggga
     9241 aaagctttga taaagatggc ttctatgagg agcttagttc tgctattgaa ctttttcaaa
     9301 gaacagggat ctcaggagaa gagatcctaa aactcgaaga aagtaagaga ctaaaggagc
     9361 tgggacttat ctatagggcc tatgaaaaca ccttgcagga gcaaggtttt taccaagacg
     9421 ctgccctaga actttttgag tcaagccctg ctcttggctt atattcttcc gatcaagttt
     9481 ggattcttgg ctataaggcc ttggactata tgcagtggaa aatgatcgat gccatcaatc
     9541 cccatacgca aatccatctt gtcctaagct atgaagagga cgatattttt atgcccacaa
     9601 gaaggaccaa agaggcccta gaaagaaatt atctttgcag ccaccattat tataaaagtc
     9661 ctaaaagtga aagggtggct ttttctaagg agcttgtcta tgatgaggga aaaaaaagag
     9721 attttgtgcc ggaagttttt tctgccaagg atccctatag cgaggcagaa tttgtaggac
     9781 ttcatattct taaaagaatg aaagaagatc ccacccttag ctatggtgac ttcaaaatac
     9841 ttgtctccga tatggatagt tatgactttg tctttcgaca aatctttacg gcccttggcc
     9901 ttccactttt tagtgatagg cggcgctcta ttcttgaaag tcctatgatt caaacctttc
     9961 tttccttgct gcgaatttac cagaggggct ttaaacgcga ggatctactg ggcttcttaa
    10021 agggatatgt ccctgagagg gactgggagg acttggatct ttttgaaaac tattgtttta
    10081 gccggggcat tgggggaaaa cgatttaaag aaagttttcc tgataaggcc atggaagaac
    10141 tccgaatcaa atttctagca gaccttatct actttgaaga ttcatttaag aaaaaagcaa
    10201 gtcctcagga ctttggcgaa cttttaatag agcttttaca tagacttgat tttgccaaga
    10261 ggctggagga agaagcagag ttttatgcaa aaaaggcaga gggcgagcag tcacagcttc
    10321 tttctcagct ttggcaaagc ctgctggaat taatttctca gataagtgca gtaggaccaa
    10381 aaaaagaact tagctttgtg gcctattgca agctaatcca cgtaggccta tccaatatga
    10441 gcctgggagt aatccctccg gcggtggata gaatacgctt agccaccctt taccgtagca
    10501 cccatgatcc tgtggactat ctgtattttt gtggaatgaa tgatggactc ctgcctcggg
    10561 aatacagtga ggctcttctt ttaaaggaag aagacaagcg tcttctgcaa gaagggggtt
    10621 ttcccctgta tgataatttt gagcataagg aagaattaga ccgcttggat ctatttaccg
    10681 ccctaagcct tgtgaaaaaa gaatatttct ttagttacgc cctagcagac atccagggaa
    10741 aaagtaaact accctctctt tttatagaaa gaattttaga gcggacaggg gcgcagtttt
    10801 actccagtgc ccatagtgat aaatacctgc aggactatta ttatctgcat ccttccatga
    10861 gttttcgcta tggtttaaag gctctgcggg aaaatagaga agaaaccatc tatggcttgt
    10921 cccaagaaaa gattaggcaa atgcgtcaaa gattagaaga agcccccctg gtccccctct
    10981 taaaaagaag ggataaaagg cttcatcttt ccatttctcg ccttgaacgc tttcgccgtt
    11041 gcccttttc
//
LOCUS       DASRNA010000120         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_853316,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000120 DASRNA010000000
VERSION     DASRNA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853316"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFD08_03240"
     CDS             complement(<1..206)
                     /locus_tag="VFD08_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZK10065.1"
                     /translation="MLRLNGLVNRREGSLLFLSKEVDKVKKIGIVLEGGGVRGAYQAG
                     ALKALYENKIKPDVITGTSIGALNG"
     gene            complement(178..1440)
                     /gene="murA"
                     /locus_tag="VFD08_03245"
     CDS             complement(178..1440)
                     /gene="murA"
                     /locus_tag="VFD08_03245"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZK10066.1"
                     /translation="MEKFVVETSLNLEGSIRVSGAKNSVLIIMAATLLAEGECVIEDV
                     PDLLDVQVMIDLLRSLGSEVHYDAQKEQLRTKVIDSSITDASYELTNRMRASVVVMGP
                     LLARRKRANVALPGGCPIGARPIDLHLKGFRRLGVDVEMGHGYVEGVVKENMIGNTIY
                     LDFPSVGATENILMAATLAKGTTTIENAAQEPEITDLASFLTKMGAKISGAGTSTIRI
                     NGVERLEGVRHQVIPDRIEGGTYMIAAAITKSRIFIDNIVPTHIRPLISKLEETGVII
                     TPQANGLLVDARAGYCGVDITTLPYPGFPTDLQAQFMVMLTISEGIHTVRETIFENRF
                     MYVNELMRMGANIRIDGSSAMVHGVSHLEGAQVRSTDLRAGAALILAGMVAIGKTEIS
                     EIYHIDRGFVSIEKKLQGIGAKIKRVSQ"
     gene            complement(1486..1770)
                     /locus_tag="VFD08_03250"
     CDS             complement(1486..1770)
                     /locus_tag="VFD08_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HZK10067.1"
                     /translation="MNKVYLDGRKMDNPAQAHSYLQESMDFAGYYGENLDSLWDELTS
                     IGSPITVKLLYKGEMLENLGDYGKKLLDTFREASEENPHLVLKERNWRFF"
     gene            complement(1763..2215)
                     /locus_tag="VFD08_03255"
     CDS             complement(1763..2215)
                     /locus_tag="VFD08_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009294914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HZK10068.1"
                     /translation="MKNKRITAFILILILISFFYRGNTPGQEVLLGSLDSLEEESYTS
                     KEEVSGYLLTYNELPPNFIRKKEAQELGWVAAQGNLWELAPGKSIGGDRFYNREGLLP
                     EKKGRLWYECDIDYSGGRRTAKRLVFSSDGLIYYTEDHYRSFTEIKYE"
BASE COUNT          602 a          562 c          425 g          728 t
ORIGIN      
        1 ccattaaggg cgcctatact ggtgccggta ataacatccg gtttgatttt attttcataa
       61 agagctttta aggctcctgc ttgataggct cctctaaccc cgccgccttc tagaacaatt
      121 cctatttttt tcactttatc tacctctttt gataaaaaaa gaagagatcc ctctcttcta
      181 ttgactaacc cgtttaatct tagcaccgat tccttggagt ttcttttcaa tactaacaaa
      241 gcctcggtca atatgataaa tttcagaaat ttccgttttt cctattgcaa ccattcccgc
      301 cagtatgagg gccgctcccg cccttaggtc agtgcttctt acctgggctc cttcaagatg
      361 agatactccg tgcaccatgg cagaggaacc atcgattcga atatttgctc ccatgcgcat
      421 tagttcattt acatacataa aacgattttc aaagatggtc tctcttactg tatgaattcc
      481 ctcacttata gtaagcatta ccataaactg tgcctgtagg tccgtgggaa aacctggata
      541 gggcagggtg gtaatatcca caccacaata gccggccctg gcatcaacaa gcaggccatt
      601 ggcctggggc gtgataatca ctcctgtttc ttctaacttg ctaatcaggg gtctaatatg
      661 ggttgggaca atattgtcga taaagattct actctttgta atggctgcgg ctatcatata
      721 ggttccccct tcgattctat caggaatcac ctggtggcgg accccttcta ggcgctctac
      781 tccattaatg cgaatggtag aagttcctgc tccagaaatt ttcgctccca tttttgtaag
      841 aaaactagcc aggtccgtaa tctctggttc ctgggccgca ttttctatgg tggttgttcc
      901 cttggctagg gtggctgcca taagaatgtt ttctgtagcc cctacgctag gaaagtccaa
      961 ataaatggtg tttcctatca tattttcttt gacaacgcct tctacatagc catggcccat
     1021 ctctacatcc actcccaagc ggcgaaagcc ctttaggtgg aggtcaatgg gtcttgctcc
     1081 aataggacag cctccgggca gggcaacatt ggcccttttt cttctggcaa gaaggggccc
     1141 catcacaaca acactggctc tcattcgatt tgtaagctca taactggcat cggtaatgct
     1201 tgaatcaatt acctttgttc ttagttgttc cttttgcgca tcataatgaa cttcagagcc
     1261 caggctcctt aaaagatcaa tcataacctg gacgtccaaa agatcaggaa catcttctat
     1321 cacacattct ccctcggcaa gaagggtagc ggccataata attaatacag agtttttcgc
     1381 tccactgacc cgaatactac cttctaaatt tagagaagtt tctactacaa atttttccat
     1441 attgtatcct cctagaatta gccttagtat accatattta tttggctaaa aaaatcgcca
     1501 attcctttct tttaatacta gatgtgggtt ttcttctgaa gcttccctaa aagtgtcaag
     1561 gagcttttta ccatagtcgc ccaaattttc cagcatttct cccttataga gcagctttac
     1621 tgtaattgga cttcctatac tggttaattc atcccaaagt gagtcaagat tttctccata
     1681 ataaccagca aagtccatgg attcttgcaa ataactatgg gcctgggctg ggttgtccat
     1741 ttttcgtcca tctagataaa ctttattcat atttaatctc cgtaaaacta cgatagtgat
     1801 cctctgtgta atagatcagt ccatcactgg aaaagacaag tcgcttggct gtgcgccttc
     1861 cgccggagta gtcaatatcg cattcatacc acagacgtcc ttttttttct ggcaggagtc
     1921 cttcgcgatt gtaaaaacgg tcgcctccta tacttttgcc tggggcaagc tcccaaaggt
     1981 tgccctgggc agccacccaa cctagttctt gggcttcttt ttttcttata aaatttggag
     2041 gaagctcatt ataggtaagt aaatagcctg agacctcctc tttgctggtg tagctttctt
     2101 cttctagcga gtctaggctc ccaagaagca cttcttgccc aggagtattt cctcgataga
     2161 aaaaactgat caaaataaga atcaaaatga aagcagtgat tcttttgttt ttcatatcca
     2221 cctcacatca atccattata cctctttgcc tcttaaaatc aactctgcta agaaaggtaa
     2281 cttcttctaa aaactatcaa tatatatgtt atataat
//
LOCUS       DASRNA010000121        14952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_37786,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000121 DASRNA010000000
VERSION     DASRNA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14952
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37786"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(550..1917)
                     /locus_tag="VFD08_03260"
                     /pseudo
     CDS             complement(550..1917)
                     /locus_tag="VFD08_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(2023..3336)
                     /locus_tag="VFD08_03265"
     CDS             complement(2023..3336)
                     /locus_tag="VFD08_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK10069.1"
                     /translation="MNENIKIYDTDLLVFGAGPSGIAAAIAAAKEGQETYLIEINNKI
                     GGVMASSPGMMLGAGYPMKKSIGGFFEDFVQRMYNHRPPLAQRRLCSLENFGEEVVYD
                     PDYATTILYEMLEEAGVKLLLNHIASKVLLENHIIKGVEVVNSQRTILLRAKTYIDCT
                     GDGDIANMAGVPSQLGDEKGRMMGASLTFFMEKVDWQNAFKDSSDPYFTSYAKEGIRE
                     GKIHKSLAQIYMVKGFREGSVYFNTVTVADLDGLEIDSIVEASNIARKRVYELGEFFQ
                     SKVAGFEKSYISNIGPLVGIRETRKLEGMHTLTYQEISQGKKFKDGIVACDNPLDEVF
                     RHEKNPFNSHLAALEGDYYTIPFRSLIPKEVKNLLFAGRNISADAKAFSSVRGMPQCM
                     IMGQSVGIGASIANQEEIPVQKIDSKKVVKRLVELGVRGIGGEKL"
     gene            complement(3883..5823)
                     /gene="lpdA"
                     /locus_tag="VFD08_03270"
     CDS             complement(3883..5823)
                     /gene="lpdA"
                     /locus_tag="VFD08_03270"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZK10070.1"
                     /translation="MAKEIRVDLLPGTDKKAKIGKIKVDKAEVVSQGEVILILEGGKT
                     TMEVKSVYEGKILTINVEEGQEVEKGSLLVTIEEFSEERTVNEIQVKKMPGSEKSAVV
                     GKINVTKGQKIKKGDLIATIEGAKIAIDLESEVDGKIIEILIEEGQEVQLNTIVAKIE
                     SKKAGLVKKEKEEDQDKDDIADDYQAELVVLGGGPGGYVAAIRASQLGKSVIIVEEDA
                     LGGTCLNRGCIPTKSMVQSTKVNNLIKNAESFGIENASGNLSMQKIIDRKNSVVQTLV
                     SGLNNSMQERGIRVLKGRAVANDKKSLLVTIGGKETIVSFDDLILAPGSIVSIPRFEG
                     VDDVDILTSNELLDLTEIPESLIIMGGRVIAMEFAFIYRKLGSKVTVIQRSNTIFPSL
                     DEDIIEVVRNSAIENGIRLVEGTNVKSIKPNIDGSKTVIFEQNGEEKFVTAQELAIAT
                     GRKPNLEGLGLEKLGVEISDKYKGIKVDDKMRTTAENIYAIGDATNIYNLAHVASKQA
                     IVAANNISGADIRMDYKAIPEAVFTEPEIGLVGMSEKMCKKEKVDYLVGKFPYMANGK
                     ALVENQTEGFIKVIANKKTRKVIGAGLIGLAATDLLSIFANLIANGNTIDEAKDVVYA
                     HPTVSETAFEAILDLDGQAIHK"
     gene            6274..6618
                     /locus_tag="VFD08_03275"
     CDS             6274..6618
                     /locus_tag="VFD08_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZK10071.1"
                     /translation="MAVNHIEDLKRCMENTGKLGEACPDVMGAFMQLHHAAVEEGTLS
                     TKTKELICVAISVSIRCNDCINAHVKAAIDTGASREEISEAIGVAVLMSGGPGTAYGA
                     IAIEAMDQFLAE"
     gene            complement(6820..7638)
                     /locus_tag="VFD08_03280"
     CDS             complement(6820..7638)
                     /locus_tag="VFD08_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HZK10072.1"
                     /translation="MRYTDHHVHTKYSPDSDASIGAYLIRAKELKLEKVIFTDHIDMG
                     AIESQFQKHIDYHEYFKSMKKLEKEYEIPIKIGVEIGYERNYKEKIDDFLNKYPFDFV
                     IAALHYGDGKDFYLGDFFKGKSQHQAYLRYFELVLEMVENFNNFDVLGHLDHIVKYGP
                     FKDKTYDYTKYKKIIDDILWALIKKGKGIELNTSGLRSKLKTTFPKEGVLKRYQELGG
                     QVITIGSDAHFNRDYYAGILQGMELLKSLGFSQISSFSHRRVKQLDISKGLDLK"
     gene            complement(7652..8251)
                     /gene="hisIE"
                     /locus_tag="VFD08_03285"
     CDS             complement(7652..8251)
                     /gene="hisIE"
                     /locus_tag="VFD08_03285"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HZK10073.1"
                     /translation="MMDIKYDKDGLVPAVAQDIATGEILMLAYMNKQSLHLTIEKGIA
                     HYYSRSRKEIWKKGETSGNIQRVRGIYYDCDGDAVLLKVNQTGVACHTGEYSCFFNEI
                     KARKEDKDTLLDLYKTILERKNKPKEASYTSYLFKEGLDKILKKVGEEAAEVIIGAKN
                     NQKELIYESSDLIYHLMVLFVNEGISPGDIYQELARRAK"
     gene            complement(8248..9003)
                     /gene="hisF"
                     /locus_tag="VFD08_03290"
     CDS             complement(8248..9003)
                     /gene="hisF"
                     /locus_tag="VFD08_03290"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZK10074.1"
                     /translation="MLVRIIPCLDVKEGRVVKGKKFKDIQDVADPELLAEFYSKAGAD
                     ELVFYDITATKERRKASLEFIEKIAGKIAIPLCVGGGVSTMENFTQILEKGADKVSIN
                     SAAVKRPRLIAEAAKKHGRNKVVLGMDVKKTGEEKWNVFIKGGRVDTGLDAIKWAIEA
                     VSLGAGEIILNSIDADGTKQGYDLELLKAVKNSVEVPVIASGGAGKMEDFSKAVLDAK
                     ADGVLAASVFHYKEIEIKEVKKYMKSKGIEVSL"
     gene            complement(8997..9704)
                     /gene="hisA"
                     /locus_tag="VFD08_03295"
     CDS             complement(8997..9704)
                     /gene="hisA"
                     /locus_tag="VFD08_03295"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZK10075.1"
                     /translation="MIIYPAIDIKDKSCVRLTQGDYKKMTVYEKDPVKVAKRWEKMGA
                     QILHLVDLDGAKDGIRSNQRVVKRILENINIPIQLGGGIRDLQGVEELLDWGVSKVII
                     GTVALNDPAWTKKTIEKHGERIIVSIDALNGLIATNGWQQLTDVKAIDFIKKLESYGL
                     RRIVYTDIEKDGMLIGPNFQIYEELAKSVSIEVIASGGVTSLEDIKRLKAMNLYGAII
                     GKALYDGKLNLEEALRC"
     gene            complement(9707..10285)
                     /gene="hisB"
                     /locus_tag="VFD08_03300"
     CDS             complement(9707..10285)
                     /gene="hisB"
                     /locus_tag="VFD08_03300"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZK10076.1"
                     /translation="MRKSKICRNTLETRIEVDLAIDGSGDIAINSGIGFFDHMLKAMA
                     FYAGFDLHINCQGDLHIDSHHTLEDIGLTLGAAFKEALGDKKGIQRFASPITPMDESL
                     VLVAIDISNRPYLVENLKFNAQRMGDLETENFKEFFQAFTVSAAISLHIDVIRGENDH
                     HKIEAVFKGLGRAIKEAAHLVGSDVVSTKGVL"
     gene            complement(10272..11279)
                     /gene="hisC"
                     /locus_tag="VFD08_03305"
     CDS             complement(10272..11279)
                     /gene="hisC"
                     /locus_tag="VFD08_03305"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZK10077.1"
                     /translation="MEKYLRKEIKNLKPYEVIDRAYSLKLDANEGIDWMDGLNRYPND
                     RSDKLRKKLAEKLGKNTNEILLGNGSSELIELVMKAYLEAGEKVVSLSPSFSMYKIFT
                     IIHKGEYLDFPLDDMEKLDTEAFIDFVKKQKPKLLILSNPNNPTGTLIPKKDIRKIIQ
                     SVDAMVILDEAYIEFSDYPKGDDSREFENLLVVRTFSKARGLAGIRLGYMIAARQTLE
                     YIERVRSPYNVSTLTQNMGLKALEKDFISKQHIELIKKERARMAKFLENKKLKPLASQ
                     ANFLFFQAPKNIFDCLWNKNILIRKFGAELEGYYRLTIGSPQENDIVIKTIEEEINAQ
                     I"
     gene            complement(11233..12564)
                     /gene="hisD"
                     /locus_tag="VFD08_03310"
     CDS             complement(11233..12564)
                     /gene="hisD"
                     /locus_tag="VFD08_03310"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZK10078.1"
                     /translation="MIPILAASKEGKKMEEILNRSQMDSKEVILKVDEILEEIKVSGD
                     KALVKFTQTYDKNKAEKLRVTEEEIQRAKKRIDKNLKDAIEGAIENISSFHKKQIKEG
                     FMIKEDKDIVLGQILLPIEKVGIYIPGGTASYPSTVMMNVIPAKLAGVKKIIITSPAD
                     KEGKIKDSVLVTADLLGVREIYKVGGAQAIGALCYGTETIPKVDKIVGPGNIYVAMAK
                     YRVSALVGIDMVAGPSEVVIIADKEANPRFIAADLIAQAEHDKRAASIVITDCKKLAK
                     ELQKELDIQLKTLERSEIAKASLNQYGAIILCENQEECFEIANKLAPEHLEILTENPF
                     EDYKKIKNAGAIFLGEYTPEPVGDYYAGPNHTLPTSKAARYSSALGVDDFYKKTSLLY
                     YSKAALKKAGKDIILLAENEGLTGHANSIKLRLEEKDGKIPKKRNKKPKAL"
     gene            complement(12565..13185)
                     /gene="hisG"
                     /locus_tag="VFD08_03315"
     CDS             complement(12565..13185)
                     /gene="hisG"
                     /locus_tag="VFD08_03315"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZK10079.1"
                     /translation="MNRLTIAIAKGRLGQKGMELFKDTEYEIVMEEGSRKLVYKNAKE
                     NIDFIFVKPCDVVTYVEKGVADLGIVGKDTILEDKKDIYEVLDLNFGNCRFVIAGFEE
                     QSLIKKNEVLTIATNYPSYVESIFKGKGQQIEIITLGGAVELAPLMGLSEVIVDIVET
                     GNTLKDNGLVILEDLEPISARLIVNKVSYRFKFDEVKKIEKVLGGM"
     gene            complement(13185..14135)
                     /locus_tag="VFD08_03320"
     CDS             complement(13185..14135)
                     /locus_tag="VFD08_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZK10080.1"
                     /translation="MQFRNMKEELAITKFKKKIEREIEEYFEDKGFSIIEPKTFQDYS
                     DCIQSGLIKNPSETVKVLGGDSKIYILRPDITTNLLGQIFAKWEREAPLKIYYNSKIY
                     RNGPRGEILQNYQMGIESLGDDEFKADKEIIEMALSVMSSLGEAYLIELGSSLFLDSF
                     FREIKLERGDEKEIRKLISKKNYHGLKVKLKLLKLENTILDDIFRMQGNIEEVIDIAQ
                     THRINEGMRSALESLTLLGKAFNSKDTKNKIKVDLSMVSELDYYDGIIFRGYCISLPT
                     KILSGGRYDKSTAKYGAKTAAIGFMIDMDLVTQVRIKELK"
     gene            14576..>14952
                     /locus_tag="VFD08_03325"
     CDS             14576..>14952
                     /locus_tag="VFD08_03325"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HZK10081.1"
                     /translation="MQNEIVTRGDLLDKNGNLVQKGWARSALLKYNRRHVAAKNFQIK
                     EWDYYAVLNDEFGISLTIADNGYFGVGIVSVYDFTIPKEWTKQVLLPFPLGRLNMPES
                     SCTGDVIFEKKDFQLKFEKDEEK"
BASE COUNT         3989 a         3195 c         2290 g         5478 t
ORIGIN      
        1 gtatttaata tctgtcttta tcttatagct cgcttatgaa ctcagtgtga acgaagtagt
       61 ctttagaaaa agaatagcta cagggcccta agtaggtgtg gggattatat tttatgtgaa
      121 ataagcttaa aagaagtatt aacccctact cctctactag aagttcataa agtctttgat
      181 attcttcatt atgtcacctt gaaataagag tgtcactgat gcagggactc ctgatatagc
      241 tgatgcgcct aaccaatcag tttataggac ggactgatag taagcacaat atagtaagag
      301 tcctgggtct ttccagatat ttttatagga ctccttaatt tatacccctt tttatcttct
      361 gtcaatttta tccccctaag cctttggctt taattgaacc tagcaggtcc ctaataaaac
      421 aaatataggt tctttttatt agaagaaaaa aagcccttat atcaggcttt taataaaatc
      481 tgtcccctct ttacttttat actagtgtgt gttttttctg tttgtagata agtccatata
      541 taatgattac tagtatatgc gcaggccttg cttttgaatg gccttttgga ggtttttaat
      601 gttaatcttt gatgtattca ccttatcctc taaggataca gctgctgcaa taccagccgc
      661 ttggcctgtt agtgcacagc atgacatatt tctaaatgca cagtgggcaa ccctatcacc
      721 agaaatggat cttcctgcca ctaatagatt gtctatcttt ttaggtaaaa tggctctata
      781 gggtatttgg aaatatcgac ctgttgttgg aaggtacaga tggtttaatc catcaacaaa
      841 ttcaggaaaa atccctatgc tatcttcaaa gcgggcttga tttaggacat cttcttttct
      901 tagatagtag tgtccttgta tttttctgct ttcacgagcc ccaattgtca tggcgaaatt
      961 tcttattcca gcctcttgaa agcccggcac cttatgtctt aataccttta ataccttaag
     1021 cacatgctct cgcccttgta tttctgctct agttaaatcc cttacattta gtggatcata
     1081 gccacttata aaaaccatat tgagctgggt gacctcaccc attttttcat tgattgtgct
     1141 ccaggtgcca ccaagggaaa cattttcatc cacctcaatt agtccttctt cgatagcctg
     1201 ggtgaagggg gtttttatat agggactaaa catatggctt tcttttcctg ttgatattag
     1261 attccagacc tccccagccc agtccttgta ggtcggtttt agttgcttat aaatatactc
     1321 tttaaatttc ttagtatcaa caccgtgaca attaaaaaca ggtgaaagat gcattaattc
     1381 ctcgctagcg gacttagtaa aaggagcgcc cgccaaggct gctatatctc catctcctgt
     1441 tcaatcgatt actcttttgg ctaaaatagc ttggcgacca gccttgcttt ctgtaattac
     1501 tcctttgatt tgattatcct ctaccatggc ttccaccgca tagcagtgta aaatcggttc
     1561 tactccggct tctaataaaa ttctatcagc aagatattta aacatctcag catctaaagc
     1621 ctgactatca gactgaattt ccttgctact ggcccccatt tcttctgcta gatcttcaaa
     1681 ttctctggca attccgcccg cttcaatggt agccttatgt cgataccagg caaaactctc
     1741 tacaccaaca agagtaatat tgcctccaaa acaagaaaat ctctccaaaa ttgttgtttt
     1801 agctcctgct cttgaagaag ctagtgctgc ggctatgccc gcgggaccgc cccctactac
     1861 taatacttct gtttcccata aaacagaaac ttcttttggc agttctttaa ttgacataat
     1921 agcaaaacct cccttatttt ataaaaaaac attcaaccgg tagaaatagt tttattaagc
     1981 caggcttatc ttaaagtgta agatgactgt ctatagttaa ctttatagtt tctctcctcc
     2041 aatccccctt acaccgagtt ccactaatct ctttactacc ttcttagagt ctatcttttg
     2101 cactggtatt tcttcctggt ttgcaatgga agctcctata ccaacacttt gccccatgat
     2161 catacattgt ggcatccctc ttactgagga aaatgcttta gcatctgcag atatatttct
     2221 acctgcaaaa agtaggtttt ttacctcttt tggtattaga gatctaaagg gaatcgtgta
     2281 ataatctcct tctagtgctg caaggtggct attaaaggga ttcttctcat ggcgaaagac
     2341 ttcatccaag ggattgtcac aggccacaat cccatctttg aacttttttc cttggctaat
     2401 ttcttgataa gttaaggtat gcattccttc tagttttctt gtttctctta ttccaactaa
     2461 gggccctata ttggaaatat aacttttttc aaagcctgct actttcgact gaaaaaattc
     2521 tcctaattca tatacacgtt ttctagcaat attactagcc tctactatac tatctatttc
     2581 aaggccatca aggtctgcta ctgttactgt attaaaatac acagacccct ctctaaatcc
     2641 ctttaccata taaatttgag caagactttt atgtatttta ccttctctaa tcccctcttt
     2701 ggcatagctt gtaaagtatg ggtcactact gtctttaaag gcattttgcc aatccacctt
     2761 ctccataaaa aaagtcaagc ttgcgcccat catccgacct ttttcatcgc ctaattgact
     2821 tgggactcca gccatatttg ctatatctcc atcccctgtg caatcaatat aggtctttgc
     2881 cctaagtagg atggtcctct gggagtttac tacttctacc cctttaatta tatggttttc
     2941 aagcaaaact ttagaagcta tatgattaag aagcagtttt actcctgctt cttctaacat
     3001 ttcatagagt atagtggtag catagtctgg atcataaacg acttcttcac caaagttttc
     3061 caaactgcat aaacgtcttt gtgcaagagg aggcctatga ttatacatcc tttgaacaaa
     3121 gtcctcaaaa aagcccccta tgcttttttt cataggatat ccagccccca gcatcatgcc
     3181 gggtgaactc gccatcaccc ctcctatttt gttattgatc tcaattaaat atgtttcttg
     3241 cccttcttta gcagcagcta tggcagcagc gataccagat ggtcctgctc caaatacaag
     3301 aaggtcagta tcatatattt ttatattttc attcattgcc tagcccccta tctcatctct
     3361 gattcgccct tgcaaaactc gcaactcacc cctatgctta tgtaaaactg tttagatttt
     3421 ctcttttata tttttttggc tagtaatagt aagtcaataa gtaaatagtt caccgggaaa
     3481 gcttcctgta gaatggttgc aagtagacag taatctaagg tggtgagccc agtaaactac
     3541 ccttatctta cactatttga gtgagtgttc ctagataaga tttgctttat aggcttgcta
     3601 gcaggtaagc tcagataaaa agatttgctt cctatagaaa agaaagtcct tggtttcaag
     3661 gactttcttg cagggctact attagctact tatactatct agctaaaagt ctatttaagc
     3721 actggttttc ttgcttatga aatacagccg cttttttgct ttttcttagc agtctggtgg
     3781 cagctcacgg attttttata aaatattctt tttgccccaa aatataggct ggattaattg
     3841 cctcagatat aatgattctt ttatccataa gctggcccgt ttttatttgt ggattgcctg
     3901 cccatctaag tctaggatgg cttcaaaagc cgtttctgat actgtggggt gggcatatac
     3961 tacatccttt gcctcatcta tggtgttgcc attggcaatt aaattggcaa atatacttaa
     4021 aagatctgtg gcagccaggc ctattaatcc tgctccaatt acttttcttg ttttcttatt
     4081 ggctatgacc ttgataaaac cctcggtctg gttttccacc aaagctttgc catttgccat
     4141 ataggggaat ttccctacta agtagtctac tttttctttt ttgcacattt tctcactcat
     4201 gcctaccagc cctatctctg gctcagtaaa tacggcctcg ggtatggcct tgtagtccat
     4261 tcttatatca gccccggaaa tgttattggc agctactatg gcttgttttg acgctacatg
     4321 ggctagattg tatatatttg tcgcatctcc aatggcatag atgttctctg ctgttgttct
     4381 cattttatca tcgactttta ttcccttata cttatctgag atctcaactc cgagtttttc
     4441 aagccccagt ccctctagat ttggttttct tcctgttgca atggcaagtt cttgtgcggt
     4501 tacaaacttt tcctctccat tttgctcgaa aataactgtt ttgctgccat caatatttgg
     4561 cttgatggat tttacatttg taccctctac cagcctaatc ccgttttcta tggcactatt
     4621 tctaacaact tcaataatgt cttcatctaa acttggaaag atcgtatttg atctttgaat
     4681 taccgtgacc ttagagccaa gctttctata gataaaggca aattccatag ctataactct
     4741 tccacccata attatcagtg attctggtat ttctgtaaga tctaagagct catttgatgt
     4801 tagtatatct acatcgtcta ctccctcaaa tctaggaatg gatacaatgc ttcctggtgc
     4861 caaaatcaga tcatcaaagc tgactatggt ctcttttcct ccaatagtaa ccagcaggga
     4921 tttcttatca tttgctactg ctctaccctt taagactctt ataccgcgct cttgcatact
     4981 attatttaat cctgatacta gtgtttgtac aacagagttt tttctatcaa tgattttttg
     5041 cattgataga tttccacttg cattttctat tccaaagctt tcagcatttt ttatcaaatt
     5101 atttactttt gtgctttgga ccatggattt agttggaata catcctctat ttaaacaagt
     5161 tcctcctaag gcgtcttctt ctactataat tactgatttt cctaattgac ttgccctaat
     5221 agctgctaca tatcctccag gtccaccacc aagaacgaca agttcagcct ggtaatcatc
     5281 tgcaatgtca tctttgtctt ggtcttcttc tttttctttt ttaactagtc ctgccttttt
     5341 gctctctatt tttgctacta tggtatttag ttggacttct tgtccttctt ctattagtat
     5401 ctctataatc ttgccatcta cttctgattc tagatcaatg gctattttag ctccctctat
     5461 tgttgctatt agatcacctt tttttatttt ttggccttta gtaacattaa ttttcccaac
     5521 cactgcactt ttttcacttc ctggcatttt tttaacttgt atttcattaa ctgttctttc
     5581 ttcggaaaac tcttctattg ttactagtaa agaacccttc tctacttctt gaccttcttc
     5641 tacattgatt gttaagatct taccctcata tactgactta acttccatgg tggtttttcc
     5701 accttctaag attaaaatta cctctccttg agaaactacc tctgctttat ctactttgat
     5761 ttttcctatt tttgcctttt tatcagttcc tggtaataga tcaactctta tttctttagc
     5821 cattttttct cctcctagat atttctatag gtaaatgcct tgtgtaattt tatcccctgg
     5881 tcaatataag cttggtcctt atctaagtca ccttgtccac tgtttttatt ttataatcaa
     5941 aagaaaaagt atatggatat tgctttttat aatcttgctg cctcttgtgt ttttttattg
     6001 gtattagttt tttacttttt ttctcttaca aggcactatt atcaatgtgt ttactcttat
     6061 attgtatcat atcttgctgt atttcttaga aggactccat ccaaagctct cccttttatg
     6121 tctactagct caaatctagt ctctgcttaa gtattccttt tcttttaagt ttgatttctt
     6181 caataataac tagccagata ttcaaatttt tgatattaac ttagcagtgt gttataattt
     6241 aaagtaacaa caacgattaa aaggaggatg tttatggcag tcaatcatat cgaagaccta
     6301 aagcgatgta tggaaaacac tggtaaacta ggagaagctt gccctgatgt aatgggtgct
     6361 tttatgcaac tgcatcatgc agcagtggaa gaaggaaccc tgtctaccaa gaccaaagaa
     6421 ctgatttgtg tagcaatttc tgtttctatt agatgcaatg actgtattaa tgctcatgtt
     6481 aaagcagcta ttgacactgg cgcaagccgc gaagaaatta gtgaagccat tggtgtagct
     6541 gttttaatgt caggtggacc tggaacagcc tacggggcaa tagcaataga agcaatggac
     6601 cagttcttag cagaataaga acttactagt aaagctctaa ccactaggac attagcaggc
     6661 ccccctatag caacagctaa aagaatcaaa ctgttgttat agggggcttg ccttaatagg
     6721 aaacaacttc ttaaaaaaag taggggctag attttagcat aaagctaact ctagcccctt
     6781 ttatcttata ctttaatggc ctatatctac taatagttct tattttaaat ctagtccctt
     6841 actaatatca agttgcttta ctcttctatg ggaaaaggaa gaaatttggc tgaagcctag
     6901 ggattttaaa agctccatgc cctgtaaaat acctgcataa taatctcggt taaaatgagc
     6961 atcagagcca attgttatga cctggccacc taattcttgg tatcttttta acaccccttc
     7021 tttgggaaat gttgttttta atttgctcct aagtccagat gtatttagtt ctattccttt
     7081 gccttttttt ataagggccc ataaaatatc atctatgatt ttcttgtatt tggtataatc
     7141 ataggtcttg tctttaaaag gaccatattt aacgatatga tctaaatgcc ctaggacatc
     7201 aaaattatta aagttttcta ccatttctaa taccaattca aaatatctta aataagcttg
     7261 atgctgtgat tttcccttga aaaagtctcc caaataaaag tcttttccat caccatagtg
     7321 gagagctgca attacaaagt caaagggata tttattcaaa aaatcatcta tcttttcctt
     7381 gtaatttctt tcatagccta tttctactcc tatttttatt gggatttcat attctttttc
     7441 aagttttttc atgcttttaa aatactcatg atagtctata tgcttttgaa actggctttc
     7501 tattgctccc atatcaatat ggtctgtgaa tataaccttt tcaagtttta attctttagc
     7561 cctaatcaaa tatgccccta tactggcatc actatcagga gagtattttg tgtgtacatg
     7621 gtggtctgta tatctcaaag taaaaacctc cttacttggc tcttcttgcc aattcctgat
     7681 aaatatctcc tggactaatc ccttcattta caaatagaac catcagatga taaatcaaat
     7741 cacttgattc atagattagt tctttttgat tgttttttgc acctataatc acctctgcag
     7801 cttcctctcc tactttttta agaatcttat ccagtccttc cttaaaaaga taggaagtgt
     7861 aggaagcttc tttgggcttg ttttttctct ctaggattgt tttataaaga tctaacagtg
     7921 tgtctttatc ttcttttctt gcctttattt cattgaaaaa acatgagtat tctcctgtat
     7981 ggcaggccac tcctgtttga tttaccttta aaagaacagc atctccatcg caatcgtagt
     8041 agattcccct taccctttga atatttccag aggtctctcc ctttttccat atttcttttc
     8101 tcgagcggga atagtaatga gctatgccct tttctattgt aagatgaagt gattgcttgt
     8161 tcatataggc tagcatcaag atttctcctg tggctatatc ttgggctacg gcaggaacta
     8221 gtccatcttt atcgtatttt atatccatta taagctcacc tctattccct ttgatttcat
     8281 gtattttttt acttctttga tttctatttc cttgtaatga aaaacgctag ctgccagcac
     8341 cccatcagcc tttgcatcta ggactgcttt agaaaagtct tccatttttc ctgcaccacc
     8401 cgatgcaatt accggcacct ctacgctgtt ttttaccgct tttaagagtt ctagatcata
     8461 cccttgtttt gttccatctg catctatact atttaatata atctcaccgg ctccaaggct
     8521 tactgcttct atggcccact ttattgcatc aagtccagtg tctactcttc ccccttttat
     8581 aaagacattc cacttttctt ctcctgtttt tttcacatcc atacctaaaa ccactttatt
     8641 tcttccgtgt tttttggcag cttctgcaat aagcctgggc cttttaacag cagctgagtt
     8701 aattgaaacc ttatctgccc ctttttctag tatctgtgta aagttttcca ttgtactaac
     8761 gccaccgcct acacaaagag gaatagctat ttttcccgct attttttcta taaattcaag
     8821 agaagctttt cgcctttcct ttgtagcggt aatatcatag aagactagct catctgcccc
     8881 tgcttttgaa taaaactcag ctaggagctc aggatcagct acatcttgga tgtccttaaa
     8941 cttttttccc tttaccactc taccttcttt tacatctaag caaggaatga ttcttactag
     9001 catctgagtg cctcctctaa atttaatttc ccatcataaa gggctttacc tataatcgcc
     9061 ccataaagat tcatagcctt aagtctttta atatcttcta gggaagttac tccacctgaa
     9121 gcaatcactt ctatagatac acttttagct aactcttcat agatttgaaa attaggtcca
     9181 attaacattc catccttttc tatatccgta taaacgatac ggcgtaagcc ataggactct
     9241 agttttttta taaaatctat tgccttaaca tcagtaagct gctgccagcc atttgtagca
     9301 atcagtccat tgagagcatc tatagaaact ataattcttt ccccatgttt ttcaattgtt
     9361 tttttagtcc aagctggatc attaagggct actgttccta taattacttt agaaaccccc
     9421 caatccaaaa gctcttcaac cccttggagg tctcttatac caccgcctag ttggatggga
     9481 atgtttatgt tttctagaat cctctttact accctttgat tgctccttat accatctttt
     9541 gctccatcta gatctacaag atgaagtatc tgcgctccca ttttttccca tcttttagct
     9601 acctttactg gatctttctc atatacagtc atttttttat aatcaccttg ggtcagcctt
     9661 acacaagatt tatcttttat atctattgct ggataaatta tcatgtttac aaaaccccct
     9721 tagtactaac cacatcacta ccaacaagat gagctgcttc ttttatggcc ctgcctagac
     9781 ctttaaagac tgcttcaatc ttatgatgat cattttctcc tcggattaca tctatatgta
     9841 gacttatagc tgctgaaaca gtaaaagcct gaaaaaactc tttgaagttt tcagtttcta
     9901 aatctcccat tctttgggca ttgaatttta agttttcaac caagtagggg cgattggaaa
     9961 tgtctattgc aactaggaca agactctcat ccatgggagt aatcggcgag gcaaagcgct
    10021 ggataccttt tttatctcct agggcctctt taaaagcagc ccccagagtc aagccaatat
    10081 cttctagggt gtgatggcta tcaatatgga ggtctccttg acaatttata tgcaaatcaa
    10141 atcctgcata aaatgccatg gcctttaaca tatgatcaaa gaatccaata cctgagttta
    10201 tagcaatatc acccgatcca tctattgcta ggtctacttc tatccttgtc tctaaagtat
    10261 ttctgcagat tttagatttg cgcatttatt tcctcctcta ttgtctttat aacaatatcg
    10321 ttttcctggg gacttcctat agtaagtcta tagtaaccct ctagttctgc cccaaacttc
    10381 ctaataagta tgtttttatt ccagaggcag tcaaatatat ttttgggggc ttgaaaaaat
    10441 aagaaatttg cttgagaagc cagaggcttt agcttcttgt tttctagaaa ctttgccatt
    10501 cttgcccgct cttttttaat tagttctata tgctgcttgc taatgaaatc tttttccaaa
    10561 gcttttaggc ccatattctg cgttagggta cttacattgt atggtgatct aacgcgctct
    10621 atatactcta aggtttgtct agctgcaatc atatatccaa gccttattcc tgctagtcct
    10681 ctagctttgg aaaaagttct tactactaga agattttcaa actcgcggct atcatctccc
    10741 ttggggtagt cagaaaactc aatataagct tcatcaagaa taaccatggc atctactgat
    10801 tggattatct ttcttatgtc ttttttagga atcaatgtgc cggtaggatt attaggatta
    10861 ctcagaataa gtagttttgg cttttgcttt ttcacaaaat ctataaaggc ttctgtatct
    10921 agtttttcca tatcatccaa gggaaagtct aggtattccc ctttatggat aatagtaaat
    10981 atcttataca tgctaaaact tggcgataaa cttaccacct tttctcctgc ttctagatag
    11041 gctttcataa caagttctat tagttcacta gaaccattgc ctaggaggat ttcatttgtg
    11101 tttttaccta acttttcagc cagctttttt cttagcttat cacttcgatc atttggatag
    11161 cgatttagtc catccatcca atctatacct tcatttgcat ctagcttaag gctatatgct
    11221 cgatcgataa cctcataggg ctttaggttt tttatttctt ttcttaggta tttttccatc
    11281 tttttcctcc aatctcagct taatagagtt agcatgccct gtgagtcctt cattttctgc
    11341 taatagaata atgtcttttc ctgctttttt tagagcagct tttgaataat atagcaagga
    11401 ggttttttta taaaaatcat ccactccaag ggcagaagaa taacgagctg cttttgatgt
    11461 aggcagggta tggtttgggc ctgcataata atctcctact ggctctggtg tatattcccc
    11521 aagaaagatc gcccctgcat tttttatttt cttataatcc tcaaagggat tttcggttag
    11581 gatttctaag tgttctggcg ccaatttgtt ggctatttca aaacattctt cttgattttc
    11641 acaaagaata atagccccat attgattaag agatgctttt gctatttcac ttcgctcaag
    11701 agttttaagc tgaatatcca gctctttttg aagctctttt gctagttttt tgcaatccgt
    11761 aataactata ctggctgctc ttttatcatg ttctgcttgt gcgattaagt cagctgcaat
    11821 aaatctagga tttgcctcct tatcagctat aatgaccact tcacttggtc ctgctaccat
    11881 atctatgcct accaaggcag aaaccctata ttttgccatg gcaacatata tatttcccgg
    11941 acctactatt ttatctacct taggaatagt ttcagttcca tagcaaagag ctcctatagc
    12001 ttgggcgcca ccaaccttgt aaatctctct aacccctaat aaatcagctg tgactaaaac
    12061 tgaatccttt atttttccct ctttatctgc tgggcttgtt ataatgattt tttttactcc
    12121 tgctagtttt gctgggatta cattcatcat cactgtagag ggataggagg ctgtccctcc
    12181 tggaatatat attcctactt tttcaatggg tagtaagatt tgtccaagga caatatcctt
    12241 atcctctttt atcataaagc cttctttgat ttgtttttta tgaaagctgc ttatgttttc
    12301 aatggcaccc tcgatagcat cttttaagtt tttatctatc ctctttttgg ctctttgaat
    12361 ttcttcttct gtgactctga gtttttctgc tttgttttta tcataggtct gggtaaattt
    12421 taccaatgct ttgtcgccac tgacttttat ttcttctaat atttcatcga cttttaaaat
    12481 cacttctttt gaatccattt gacttctatt taaaatttct tccatctttt ttccttcttt
    12541 gcttgcagct agtataggaa tcatttacat acctcctaat actttttcta tttttttcac
    12601 ttcatcaaat ttgaacctat agctgacctt attaactatc agtctggcac ttatgggttc
    12661 aagatcttct aaaatcacca ggccattatc ttttaatgta tttcctgttt ctactatgtc
    12721 tacaatcacc tcacttaaac ccattagggg ggcaagttct actgcccctc ctagtgtaat
    12781 aatctcgatt tgttggcctt ttcctttaaa tatcgattct acataggagg gatagtttgt
    12841 agctatggtt aaaacctcat tctttttaat taggctttgt tcttcaaaac ctgctataac
    12901 aaatctacag ttgccaaagt taagatctag aacttcatat atatctttct tgtcttctaa
    12961 gattgtatct ttacctacta ttcctaaatc ggctactcct ttttcaacat aagtcactac
    13021 atcacagggt ttaacaaata taaaatcaat gttttccttt gcatttttgt acactaattt
    13081 tctagaacct tcttccataa caatctcata ttccgtatct ttaaaaagtt ccatgccctt
    13141 ttgccccagc cgtcccttgg ctatagcaat ggtaagtcta tttatcattt caactccttt
    13201 attctaacct gggtaaccaa gtccatatct atcataaaac cgattgccgc agtctttgcc
    13261 ccatactttg ccgttgattt atcatagcgg cctccactta atatttttgt gggaaggcta
    13321 atgcaatagc ccctaaaaat aattccgtca taataatcca gttctgaaac catggataaa
    13381 tcaaccttta ttttattctt tgtgtcttta ctattaaaag cttttccaag aagggttaaa
    13441 gattctaacg cactcctcat cccttcattt atcctatggg tctgggctat atctataact
    13501 tcctctatat ttccttgcat cctaaatata tcgtctaaga tggtgttttc tagttttaaa
    13561 agctttagtt ttacttttag cccatgatag tttttcttgg aaatgagctt tcttatctct
    13621 ttttcatccc ctctttccag ttttatttct cggaaaaaag aatctaagaa tagactagat
    13681 cccaactcta ttaaatatgc ctcacctaaa gagctcataa cagataaggc catttcaatt
    13741 atttccttgt ccgctttaaa ttcatcatct cctaatgatt caattcccat ttgatagttt
    13801 tgtaaaattt ctcctctagg accatttcta tagatcttgg agttataata aattttaaga
    13861 ggggcttctc tctcccactt agcaaagatt tgacctagta aattggtggt tatatctggt
    13921 cttaaaatat agattttaga atccccaccc aatactttta ccgtttccga ggggtttttt
    13981 attaaacctg attgaataca atcgctataa tcttgaaatg ttttaggctc aataatagaa
    14041 aaacccttgt cctcaaagta ctcttctatt tccctttcaa tctttttttt aaactttgta
    14101 attgctagtt cttctttcat atttctaaac tgcatctttg ccgctccttt tatcaaaaaa
    14161 caaaaaacca tataggctac ctatatggtt aaaaagtact ttccatcata gatatatgcc
    14221 caagtttttt caaaacttaa gagagcatgt atctatgaac tatttatcta tcaactagtt
    14281 caactttaca tacccttcca cctatgatga tggttataaa atttattgtt tattttttga
    14341 tttattttca ttataatgcc ccgctctttt tatgagtctg tttaattgta gcattttaag
    14401 tctaaaacag caagaattgc ttttagcatt aaccctatat ttatttactg gtcaccggcc
    14461 ttgggaaaag cacagctagc aagggaaata tatttgataa tgcagggcca ttactataaa
    14521 ataatagtag aaggtattta ctggactcat aagctatcaa ggagggtaag tgaatatgca
    14581 aaatgaaata gtcactagag gcgatttatt agataaaaac ggcaacctag ttcaaaaagg
    14641 ttgggccaga tcagctctac tcaaatacaa tagaagacat gtagctgcca aaaattttca
    14701 aattaaagaa tgggattatt atgctgtcct taacgatgag tttggaatat ctttaactat
    14761 tgcagataat ggatattttg gtgtgggtat tgtgagtgtg tatgacttta ccattcccaa
    14821 ggaatggaca aagcaagtcc tccttccctt tcccctggga agactcaata tgcctgagag
    14881 ctcctgcaca ggcgatgtga tcttcgaaaa aaaggatttc cagttgaaat tcgaaaaaga
    14941 tgaggaaaaa ag
//
LOCUS       DASRNA010000122         8214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_189097,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000122 DASRNA010000000
VERSION     DASRNA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189097"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFD08_03330"
     CDS             <1..493
                     /locus_tag="VFD08_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HZK10082.1"
                     /translation="TKIYINPTGRFVIGGPQADTGVTGRKIIVDTYGGYSRHGGGAFS
                     GKDPTKVDRSASYAARHAAKNLVAAGLAKKVEIGLSYAIGVAKPVSIYVDSFGTGLVS
                     DKRLGEILDKHFDFRPASIIENLDLRRPIYRQVASYGHFGRDELDLSWERLDKVEALK
                     KEI"
     gene            549..2687
                     /locus_tag="VFD08_03335"
     CDS             549..2687
                     /locus_tag="VFD08_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HZK10083.1"
                     /translation="MEFSGNVAEIIFESPRDYYKVLLVEGEEEVLVVQGNLPGIGLGD
                     YAVFKGDMEVHPRFGEQLKVHSFEFSQPTQVDSIEAFLSSGHIRGIGPVIASRIVEAF
                     GAKTLDVMNYEIDLLRRVPGIGQKTFEKIKADYLAMGTKKDMILYIQNLGFSPFLTAR
                     ILSALGESARSIIEQNPYRLMEIEGINFESCEKIARKSGFSLDDKRRLLAFMQDHLQK
                     ALYQGHIYLPKEILLEEMGRLEIFTEEDLEGIFVEGHLIKEKDRIYLPHAYEAEGRVA
                     ADLLRISKSPLEEKILDEDLLEEDLGLALSEEQVQALRTTLKSPLSIITGGPGTGKTT
                     LIHALVKIFPEGLSLCAPTGRAAKRMEETTGRQAVTIHRLLEYRYDEEKALLGFDRNR
                     DNPLEAKVLIVDEVSMVDIFLMKQFLEAFPDQGRLVFLGDEDQLPSVGPGRVLADMIS
                     SGTLAVSRLEQIFRQEEDSLIPANSAKVLRGEEDLLKSSKGDFFMIPTQGPQGLVDLV
                     KNRLAPYYGFDPLWEIQVLTPTKGGIMGTRSLNRALQEALNPYKGGPKIHHGERVFQI
                     GDKIMQTRNNYQTEWEDQKTLERGQGIFNGDIGEVIDIVKGEMVILFDRIRLARYSGD
                     QLMDLEHAYAMTIHKSQGSEFDCVVLAAQNVPPVLCNRNLLYTGMTRARKLLVIYYEN
                     RSLRQMIKKVDSFERYSALGELLADYNQED"
     gene            2732..3337
                     /locus_tag="VFD08_03340"
     CDS             2732..3337
                     /locus_tag="VFD08_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10084.1"
                     /translation="MALFGFYRCELCNRELFEEKYFCSKCMKELEVFLQMDRREEEGY
                     YVYSLFFYQGAIIDLIRKWKFHGSPYLVEPLSQLLSSFILENDLHVEAFSYIPMHRKK
                     RLIRGFDPMEDLSRDLARRMKKEHHQVLSRKRNTKALYKMKAEERKKELKDCFSPLSG
                     AQNLRLAIVDDIFTSGATTKEAARSLFQAGYEEFYFLIIAK"
     gene            3428..3958
                     /gene="raiA"
                     /locus_tag="VFD08_03345"
     CDS             3428..3958
                     /gene="raiA"
                     /locus_tag="VFD08_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZK10085.1"
                     /translation="MKFEISGKNYHVDDDLRNRVEDKLSKYNPYFNENVTAIVNFSQV
                     KNLNNVEITIPLKNGAIIRVEEQSFEMLTSIDRAVDKLDRQIRKHKTALRRRYQNHDS
                     IRFEEIPEVEEETEEIQIVRTKNFPIKPMNPEEAVMQMELIGHDFFVFLNSETDEVNV
                     VYKRKSSGYGLIEPIL"
     gene            4125..5507
                     /locus_tag="VFD08_03350"
     CDS             4125..5507
                     /locus_tag="VFD08_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZK10086.1"
                     /translation="MVYESAKKSIDNKTEKLSELAKKIWDYPELAFNEHRAASWTADF
                     LQEEGFEVETSYAGLPTAIKATWGSGKPVIGFLGEYDALPGMSQKVSSQKEALKEGGP
                     GQGCGHNLLGVAHVGAVLGMKAAMEEKNLPGTIIYYGCPAEEVLTGKGFMARGGAFKE
                     MDLVMAWHPATRNGVSLGTMTGLNSARFHFKGRTAHAGGDPHNGRSALDAVELMNVGA
                     QYLREHVTDDVRIHYIITEGGVAPNIVPDRASSWYYVRALSREAVVETYNRLLKIAEG
                     AAQMTETQVEVEYLGGCYNTQQNKVLAELVHKTMEETELPQWTEEEIDFARKLNKVSA
                     NYDELVAKGKASKEEQIHSQVDPITNRNGYGSTDVGDVQHLVPGVTFTSATYNIGAAG
                     HSWQVTACSGSSIGIKGMILAAKVMAVSGLKLIEDPKLVEAAKKEFDETMKGRSYECP
                     ILAETPLPQP"
     gene            5585..6271
                     /locus_tag="VFD08_03355"
     CDS             5585..6271
                     /locus_tag="VFD08_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZK10087.1"
                     /translation="MKLLIKGKTKDLYQVEDGLYKLHFKDDVTGAEGAFDPGANQVGL
                     VMEGAGKAGLRMSKYFYEKFNDMGITTHYVSSDLEENTMTVYPAESFGKGVEVILRYR
                     AVGSFVRRYGLYAKEGMALDAYVEFTLKDDKRGDPLITKEALEALGIMRAEEYQELTR
                     MTKKLGAIIREDLAKKGLELYDMKFEYGRIGKEKKLALIDEISGGNMRVYKDGLYVEP
                     LTLEKVFLGD"
     gene            6273..7754
                     /locus_tag="VFD08_03360"
     CDS             6273..7754
                     /locus_tag="VFD08_03360"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZK10088.1"
                     /translation="MKTDILLSRIKVKTCYGTFPEKVDHISLDSRQVKKGGVFVARVG
                     FNVDSHRFIPDVISQGCRFIVADRYLELPEDVGLVLVKDTPRVASLLAQYIYDFPSSK
                     MHVVGVTGTSGKTSTATMIHSLLGLVEEKSAIIGTNGFSVGEERTLNINTTPETTLLT
                     QQFAQAVEKKAQHMVMEVSSHALSLGRTAGIDYDISIYTNLSHEHLDFYRDLYHYGYT
                     KGMLLSQMGSDLRREKYVILNADDPFLEEMRWESPYEILTFSLKDKEADFYAYNLKEG
                     PEGTAFSLSCPEGEFALVSPYLGTFNVENLLCALMTLWLKGFAMEEILPLVEKMPPIE
                     GRLQFVGKEELPITLLVDYALTPDGLSRLLDAVRPLVNQRLILLTAMWGMGRDITKAP
                     KMGEISSVADYVVFTNNHPGDDDRQRLVDELEKGITHKNYKKFLERKDAIEHAIEVSQ
                     PGDVVLLSGRGSEPFMYVEKMKRIPFRDDLIALEFARKKYNDQ"
     gene            7816..>8214
                     /locus_tag="VFD08_03365"
     CDS             7816..>8214
                     /locus_tag="VFD08_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK10089.1"
                     /translation="MSYFALYRKYRPEDFYDFAGQDEITKSLRYQVKTSTFGHCYLFS
                     GIRGTGKTSMAKVFAKAVNCLNPKEGNPCGACDSCISAKKGLSLDVVEMDAASNNGVD
                     DIRELRENARFLPAGSKYKVYIIDEVQMLST"
BASE COUNT         2516 a         1593 c         1993 g         2112 t
ORIGIN      
        1 tacaaagatc tatattaatc caacaggccg ctttgttata ggtggccccc aggctgatac
       61 gggggttaca ggaagaaaga ttattgttga tacctatggt ggttatagcc gccatggcgg
      121 gggagctttt agtggcaaag atccaacaaa ggtggataga tcagccagtt atgctgcccg
      181 tcatgcagcc aagaaccttg tggctgctgg cctggccaaa aaagttgaaa taggactaag
      241 ctacgccata ggagtggcca aacctgttag catctatgta gacagctttg gaactggtct
      301 tgtcagtgat aagaggctag gagaaatact tgataaacac tttgatttta ggccagcttc
      361 tattatagaa aacttagacc ttcgtcgacc catctataga caagtggcaa gttatggcca
      421 ctttggcagg gatgaactgg accttagctg ggagcgcctg gacaaggtag aagcactaaa
      481 aaaagaaatt tgaaggtaaa acccctttag caggggtttt tttctgttat actaaggcag
      541 aggtaaatgt ggaattttct ggaaatgttg ctgaaataat atttgaaagc cctagagact
      601 attataaggt cctgcttgta gaaggagagg aggaagtcct tgtagtgcaa ggaaatcttc
      661 ctggaatagg actaggggac tatgctgttt ttaaagggga tatggaagtc catccccgtt
      721 ttggcgagca attaaaggtc cattcctttg aatttagtca gcccacccag gttgatagta
      781 ttgaagcctt tttaagttca ggacatataa gaggaattgg ccctgttatt gccagtcgta
      841 ttgtggaggc ctttggggcc aagaccctgg atgtgatgaa ctatgagatt gatcttttac
      901 gccgggttcc tggtattggc caaaagacct ttgaaaaaat caaagcagat tatctggcca
      961 tggggacaaa aaaagacatg attttgtata tccaaaatct aggctttagt ccctttttaa
     1021 cggccagaat cctatcggcc cttggagaaa gtgccagaag tattattgaa caaaacccct
     1081 ataggctgat ggaaatagaa ggaataaact ttgagagctg cgaaaaaatt gccagaaaat
     1141 ctggtttttc cctggatgat aaaagaagac tcctggcctt tatgcaggac caccttcaaa
     1201 aagccctcta tcagggtcat atctatcttc ccaaggaaat cctcctagag gaaatggggc
     1261 gcctggaaat ctttactgaa gaagaccttg aagggatctt tgttgagggc catcttataa
     1321 aagaaaaaga ccggatctat ctgccccatg cctatgaggc agaaggaagg gtggctgcgg
     1381 atctactgcg cattagtaag agtcctctag aagaaaaaat tcttgatgag gacctgctag
     1441 aagaagacct tggacttgca ctctcagagg aacaagtcca ggcccttaga accaccctaa
     1501 agtctcccct aagtattatt accggaggcc caggaacagg caagaccacc ttaattcacg
     1561 ccctagtcaa gatctttcct gagggccttt ctctttgcgc ccccactggt agggcggcca
     1621 aaagaatgga agagaccaca ggcaggcagg cagtaaccat acatagatta ttagagtatc
     1681 gctatgatga agaaaaggcc cttttaggct ttgatcgcaa tcgagacaat cccctagagg
     1741 caaaggttct tattgttgat gaagtcagta tggtggatat ttttcttatg aaacagtttt
     1801 tggaggcctt tcctgatcag gggcgtctgg tttttttagg cgatgaggac cagctaccct
     1861 cagtgggtcc aggtagagtc ctagcagata tgatttcttc tggaaccttg gcagtgtcaa
     1921 gactagagca aatctttagg caggaagaag actccctaat ccctgcaaat tccgccaagg
     1981 tccttagggg agaagaagac ctattaaaaa gcagcaaggg tgattttttt atgatcccca
     2041 cccaggggcc gcagggcctg gtagatttag ttaaaaaccg cttagccccc tattatggct
     2101 ttgaccccct atgggaaata caggtattaa ccccaaccaa gggcggaata atggggaccc
     2161 gctcccttaa ccgcgcccta caagaggccc ttaatcccta taagggaggt cctaaaatcc
     2221 accatggaga gcgagtcttt caaataggcg acaagattat gcaaacgcga aacaactacc
     2281 aaacagagtg ggaggaccaa aagacccttg aaaggggcca aggcatcttt aatggagata
     2341 taggggaagt gattgatatt gtaaaaggcg agatggtcat cttatttgat cgaatcaggc
     2401 tggcccgcta cagtggagac cagctgatgg accttgaaca cgcctatgcc atgaccatcc
     2461 ataagagcca gggcagtgag ttcgactgtg tggtcctggc cgcccaaaat gtacccccag
     2521 tcctatgcaa tcgcaatctt ttgtatacag gaatgactcg ggctcgaaaa ctcctagtca
     2581 tttattatga aaatagaagc cttagacaga tgataaaaaa agttgatagc tttgagcgct
     2641 atagcgccct aggagagctc ctagcagatt acaatcagga agactagtga aaagctagtc
     2701 ttttttagtc tagacaggct aaactcagac tatggcatta tttggttttt atcggtgtga
     2761 gctctgtaat agggagcttt ttgaagaaaa atatttttgc tccaaatgta tgaaggagct
     2821 agaagtcttt ctgcagatgg atcgtagaga agaggagggc tactatgtct actccctctt
     2881 tttttaccag ggggccatta tcgatttaat tcgaaaatgg aaatttcatg ggtcccccta
     2941 tcttgtagaa cccctaagcc agcttctaag tagctttatc ctagaaaacg acctccatgt
     3001 ggaagccttt agctatattc ccatgcatag aaaaaaacgc ctgattaggg gttttgaccc
     3061 tatggaggat ctttccagag accttgctag gcgaatgaaa aaagagcatc atcaagttct
     3121 ttcaagaaag cgaaatacca aggcccttta taagatgaag gcagaagaaa gaaaaaagga
     3181 gctaaaggat tgttttagtc cactttctgg agcgcagaat ctacgccttg ccattgtgga
     3241 tgatattttt accagcggcg ccacaacaaa agaagcggct cgtagtcttt ttcaagcagg
     3301 ttatgaggag ttttattttt tgattatagc aaaataagag atttgttttg cactttgggg
     3361 tataataaat ataaggtaat atttactacc taggcatgaa actatttgaa taggaagagg
     3421 tttaatcatg aaatttgaaa ttagtggtaa aaattatcac gttgatgatg atcttcgtaa
     3481 tcgggtggaa gacaaactca gcaagtacaa tccatatttt aatgagaatg ttactgctat
     3541 agtgaatttt tcacaagtaa aaaatctcaa caatgtagaa atcactattc cgctaaaaaa
     3601 cggagccatt atccgagtgg aagagcaaag ttttgagatg ctaacaagta tagacagagc
     3661 ggtagataag ctagaccgtc aaattagaaa acataaaaca gccctaagaa gacgctatca
     3721 aaaccatgac tccatccgtt ttgaagaaat acctgaggtg gaagaggaaa cagaagaaat
     3781 tcaaattgtt cgtacaaaga actttcctat caagcctatg aatccagaag aagcagttat
     3841 gcaaatggag ttaataggcc atgatttctt tgtttttttg aactctgaaa ccgatgaagt
     3901 caatgttgtt tataaaagaa aaagcagtgg ttacggttta attgagccaa ttctttagta
     3961 aaaaagatgg gactgtttct ataagtaaaa gataaataat cttagccaaa tccccagagt
     4021 tcattattct ggggattata ttttttatat ctagatgtta gggctaaatt ggggtaagat
     4081 aaaggaaaag aaataatagc caataataaa ggaggccact tataatggtg tatgaaagcg
     4141 caaaaaaatc aattgataat aaaactgaaa aactctcaga attagcaaag aaaatatggg
     4201 actatcctga gcttgccttt aatgagcata gggccgcttc ttggacggca gactttttac
     4261 aagaagaagg ctttgaagtt gagacgtcct atgctggtct acccacagca attaaagcca
     4321 cctggggaag cggcaagccg gtcattggat ttttaggtga gtatgacgcc cttcctggaa
     4381 tgagtcaaaa agtttctagc caaaaagaag cccttaaaga gggcggacct ggccagggct
     4441 gtggacataa tcttttaggc gtggcccatg taggggcagt tcttggtatg aaagcagcca
     4501 tggaagaaaa aaatctgcca ggaaccatta tttactacgg ctgtcctgcc gaggaagttc
     4561 ttactggcaa gggctttatg gccaggggcg gggcctttaa ggagatggac cttgtaatgg
     4621 cttggcatcc tgcaacccga aatggggtta gcctggggac catgacgggt cttaatagcg
     4681 cacgctttca ttttaaggga cgcaccgccc atgcgggagg agatcctcac aatggtcgaa
     4741 gtgccctgga tgctgtggaa ctaatgaatg taggagccca gtatttaaga gagcatgtaa
     4801 ccgatgatgt gcgaattcat tatattatca ctgaaggcgg cgttgcccct aatatcgttc
     4861 cagaccgcgc ctcttcctgg tactatgtgc gggccctatc ccgtgaggcc gtagtagaaa
     4921 cctataatag gctgttaaaa atagcagaag gggcagccca aatgacagaa acccaggtag
     4981 aagtagagta tctagggggc tgttataata cacaacaaaa taaggtcttg gccgagctag
     5041 tccataaaac aatggaggaa accgagcttc cccagtggac agaagaagaa atagattttg
     5101 ccagaaaact caataaggtc agtgccaact atgatgaact tgtggccaag ggcaaggctt
     5161 ctaaagagga acaaattcac agccaggttg atcccattac aaatagaaat ggctatggat
     5221 ctaccgatgt gggcgatgtc cagcatctgg tgcctggtgt tacttttact agcgcaactt
     5281 acaatattgg ggctgctggt catagctggc aggttacggc ttgctctggt agttcaattg
     5341 gcataaaggg aatgattttg gctgctaagg ttatggcagt ttctggccta aagctaatag
     5401 aagaccctaa gctggtagaa gcagcaaaaa aagaatttga tgaaaccatg aagggaagaa
     5461 gctatgaatg tcctattctt gcagaaactc cccttcctca gccataaaaa aaagagtttc
     5521 gactcttttt tttcttgctt gcaaggcttt gaattggtac tattaagggg aaatgaggga
     5581 aaaaatgaaa ttactaataa aaggaaaaac aaaagacctc tatcaagtag aagatggtct
     5641 ctataaactt cactttaaag atgatgtaac aggagctgag ggcgcctttg atccaggggc
     5701 caaccaggtg ggccttgtga tggagggggc gggaaaagcc ggccttcgta tgagtaagta
     5761 tttttatgaa aaatttaatg atatgggaat tactacccac tacgtgtctt cagatctaga
     5821 ggaaaacacc atgacggttt acccagcaga gagttttggt aagggagtag aggtaatcct
     5881 tcgctacagg gccgtgggta gctttgtgcg ccgatatgga ctctatgcta aggaaggcat
     5941 ggcccttgat gcctacgtcg aatttaccct aaaggatgat aagagggggg atcctcttat
     6001 taccaaggaa gccctggagg ccctggggat tatgagagca gaagagtacc aggagctgac
     6061 caggatgaca aaaaaactag gagccattat tcgagaggac ctggctaaaa agggcctgga
     6121 gctctatgat atgaaatttg aatatggccg aattggaaaa gaaaaaaaac ttgctctgat
     6181 tgatgaaata tccggtggaa atatgcgagt gtataaagat ggcctatatg tagagcccct
     6241 tacactggag aaagtatttt taggtgacta ggatgaagac cgacatactc ttatctcgga
     6301 ttaaagtaaa gacctgctat ggcacttttc ctgagaaagt agatcatatc agtctagatt
     6361 caagacaagt aaaaaagggg ggagtatttg tggccagggt tggttttaat gttgactctc
     6421 accgctttat tcctgatgtc atctcccagg gttgccgctt tattgtggcg gaccgctatc
     6481 tagagctgcc agaagatgtg ggccttgtgc tagtaaaaga tactcccagg gtggccagct
     6541 tactggccca gtatatttat gattttccca gcagcaagat gcatgtggtg ggagtaactg
     6601 gcaccagtgg caaaacatcc acggcaacaa tgatccactc cctgttgggc ctagtggaag
     6661 aaaaatcagc tattattgga accaatggtt tttctgtagg agaagaaaga accctaaata
     6721 tcaataccac accagaaaca accctcctaa cccagcagtt tgcccaggct gtggagaaaa
     6781 aagcccagca tatggtaatg gaggtcagca gtcacgccct aagcctggga agaacagcag
     6841 gcattgatta tgacatttct atttatacca atctaagcca tgagcatctg gatttttatc
     6901 gtgaccttta ccactatggc tataccaagg gcatgcttct ttctcagatg ggaagtgatc
     6961 tgcgccggga gaaatatgtc atccttaatg cagatgatcc cttcttagag gaaatgcgct
     7021 gggagagtcc atatgaaatc cttacatttt ccttaaagga taaggaggca gacttttatg
     7081 cctataatct taaggagggc ccagagggca cagcctttag tctaagttgc cctgagggtg
     7141 agtttgcact tgtgtcaccc tacctaggaa ccttcaatgt agaaaacctc ctctgcgccc
     7201 taatgaccct ttggcttaaa gggtttgcta tggaagaaat acttcctctg gtggaaaaga
     7261 tgcctcccat tgaaggccgc ttacagtttg tgggaaaaga agaacttccc attaccctac
     7321 tggtggacta tgccctaacg cccgatggac taagtcgcct gcttgatgcg gtcaggcccc
     7381 tagttaacca gcgcttgatt cttttaacgg ccatgtgggg aatgggtcgg gatataacca
     7441 aggcccctaa aatgggggaa atatcttctg tggccgacta tgttgttttt accaacaacc
     7501 atccaggaga tgatgatcgc cagcgcctgg tggatgaact agaaaagggc attactcata
     7561 aaaactataa gaaatttctg gaaagaaaag acgccattga gcacgccata gaagtcagcc
     7621 agccaggaga tgtggtcctt ctttcagggc gtggcagtga accctttatg tacgtagaaa
     7681 aaatgaaaag gattcccttt agagatgatc ttattgcact ggaatttgct aggaagaaat
     7741 acaatgacca gtgaatttat ggtatgatag aaaagagtct gttacagact cttttctata
     7801 gagccaggag ggaagatgag ctattttgcc ttatacagaa aatataggcc cgaagatttt
     7861 tatgattttg cagggcagga tgaaataaca aaaagcttgc gctatcaagt taaaactagc
     7921 accttcggtc actgctatct ttttagtggg attcgaggaa cagggaaaac ttccatggcc
     7981 aaggtttttg ccaaggccgt aaattgtctt aatccaaaag aaggcaatcc ttgtggagcc
     8041 tgcgatagct gcataagcgc taaaaaaggc ctttctctag atgtcgtaga aatggatgct
     8101 gctagtaata atggagtaga tgatatccgt gagttaagag aaaatgcgcg ttttttgcct
     8161 gcgggaagta aatataaagt atatataata gatgaagttc agatgctctc tacc
//
LOCUS       DASRNA010000123         9686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_189186,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000123 DASRNA010000000
VERSION     DASRNA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189186"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..695)
                     /locus_tag="VFD08_03370"
     CDS             complement(63..695)
                     /locus_tag="VFD08_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10090.1"
                     /translation="MKKMYSDIFKLFFGLFLYSLGMVVGYQAHIGYAPWEAFHVGLSN
                     VTGLSIGQAGILVGAIIMVFVVIKKEALGYGGILNMILIGLQFDLILSSGLVPISASF
                     VQSLIYVFIAMIIISFATYFYISAAFGAGPRDALMVFLSRRFKISNGFARRILEIVVT
                     FFGYLMGGQVWIGTLIFAFVTGVIMDFIFTKLKFDPKTIEHRLIRNSPAA"
     gene            811..2172
                     /locus_tag="VFD08_03375"
     CDS             811..2172
                     /locus_tag="VFD08_03375"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZK10091.1"
                     /translation="MQKEILKILLEHMHEGVHVVDQEGNSLIYNEALSKIEGLLPEEV
                     LGKKIKDLFPYLNDESSTLIQAMHTGKKQYQNLQDYTNFRGERISTMNRSYPIIKEGK
                     IIGAIELSKDVSHLAELSKEVGKFKGKNKKNFKPNYYTFDHIVGESPAIKEALRRAKR
                     CAKSDSTVLIYGATGTGKELFAQSIHNASRRKAHPFVAVNCGALPGTLLESILFGTVR
                     GAYTGSVDSRGLFEQADGGTLFLDEINSMSLELQSKLLRVLQESSIRKVGGEKEHYID
                     VRVIAATNKDPLIQVEEGSLRKDLYYRLNVLPLYLPELRERVGDIEILSKYFLEKNLE
                     ENQQIKGLSEELIKNLQRHPLPGNVRELEHLIKAAINLKDDDGEHLQISDMPNSYFFE
                     REMGELYNLEDRPLQEYLEELERNLVALAFAKENSNVTRAAKRLGISRQSLQYKLKKY
                     SIE"
     gene            2308..3348
                     /gene="ord"
                     /locus_tag="VFD08_03380"
     CDS             2308..3348
                     /gene="ord"
                     /locus_tag="VFD08_03380"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HZK10092.1"
                     /translation="MKENVRVIIWGPGAMGGGMAKMLLKKKGVEIVGAIGNRSKGKDF
                     YQHIGAEQGNNPDLIIGSAEDVIKPGAADIVMLATDSFTKASFPKMKTILENKMNCIT
                     TAEEMAYPKAQTPELAEELDRLAKENGVTILGTGINPGLIMDLLVVMWTGCMEEVEEI
                     TSRRVNSLSPFGELVMHEQGVGITVEEFNERKAAGKMTGHVGFAESVQMIADGIGWKL
                     DKFEQDMEPIVTDVDRKSSHGFAKAGNLAGVNMTAEGYVDGVARIHMQHPQQIEPEQV
                     GINTGDYVTIKGSPNVNMANSPEIEGGIGTYAMCVNMIPHVINSRPGLKTMLDLPCPR
                     AIMGDMRDLIEE"
     gene            3358..3654
                     /gene="ortA"
                     /locus_tag="VFD08_03385"
     CDS             3358..3654
                     /gene="ortA"
                     /locus_tag="VFD08_03385"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HZK10093.1"
                     /translation="MIKKNTWVRIHDIVLKPEERADNIPEDTKAVPLEFWTKGYLLED
                     ANMGDAVKVKTKAGRIVEGKLVEVDPQYQLDYGKLVPELLRVGEDARRILREGK"
     gene            3651..5051
                     /gene="ortB"
                     /locus_tag="VFD08_03390"
     CDS             3651..5051
                     /gene="ortB"
                     /locus_tag="VFD08_03390"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HZK10094.1"
                     /translation="MKNYNDLMARKNEIMKTAVGIDYDTFELEGCAFDFERMMRETGY
                     TIEELREIQEYFFVGHTPIIEMYNLTELARKYAKPGYGARIMIKDEATNPSGSFKARR
                     ASIAVHQAKKLGYKGIMAATSGNYGAGVASLAAKVGLDCIVVQETYDSRHVGQPEIVE
                     KARACEAYGAEVMQLTVGPELFYVFLDILEDTGYFNASLYTPFGIAGVETLGYEISMD
                     MREKYGKDPEVVVVTNAGGGNLTGTARGLIKAGATDTKIVAASVDLSGLHMASDTDFN
                     RKSFTTGHTGFGIPFCTNPDRSDVPRSAARPMRYIDEYYTIGQGSVFYMTELLANLEG
                     LERGPAGNTSLAVAFKLAQEMKEDEIILVQETEYTGAGKHINPQLSFARDHGIEIYFG
                     DTEDEVPGKNIILPEDPGKIDVVELDLMGIKRSYMRNILRTKEGEFSDVEIKYLCEEI
                     KLNEEELHKLISELRE"
     gene            5062..5430
                     /locus_tag="VFD08_03395"
     CDS             5062..5430
                     /locus_tag="VFD08_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009006403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HZK10095.1"
                     /translation="MKGFRREDDYLSRRESIANLSDEELKDRFWDNIEKIVDPLLDQA
                     YKYTSPSIERSILLRMGFSSLEAKPLVEMAIDHGMIKHGVGHVVYLVSKKLDLPIRDA
                     GMKLIEGEYWDLVKEELGGK"
     gene            5430..7643
                     /gene="oraE"
                     /locus_tag="VFD08_03400"
     CDS             5430..7643
                     /gene="oraE"
                     /locus_tag="VFD08_03400"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HZK10096.1"
                     /translation="MELKPNEKLDIHEILKDLENYEPKRRGWAWREKVEDLEMDGHIY
                     KECSTPLKQSVGIPAAVQYFDNLDPQPQESITSEIASGRFEDDIRRMRMAAWHGADHL
                     MVIRTAGQSHYDGLIEGTPQGIGGVAITRKQVRAQRKALDIIEEEVGRPINYHSYVSG
                     VAGPEIAVLFAEEGVNGAHQDPQYNVLYRNINMVRSYVDAAESKKIMAWADINQIDGA
                     HNANATARDAWKVMPELLVQHGINSLFSEKVGMKPSNISLSTVPPTAAPIPTMRYDLP
                     YAVALRQFFDKYKMRAQMNTKYITSSSREASVTHVMNMLISRLTSADIQSTITPDEGR
                     NVPWHMFNIEACDTAKQSLVGMDGLLEMVELREDGYLPAKVREIQERAVLYLEEMLEM
                     GGYYESVERGMFVDSGEYPERNGDGIAREIQGGIGAGSVYERADNYMAPVTSHYGYNN
                     TKQYGVESENAAELIGGSTFEDRSKIQYIDELDPVDNVNTRLAEKEAYRNTNMIMPEV
                     EWLADGIVQLELLLPVPMQVAKYAALEYAKHMRMEDAEVVHLECMQPAEGTRVQLKGK
                     VNFAVDIDSLEIPPEPKIYSDDELWKELEEHPMKVVAGTVGEDEHSVGLREIIDIKHG
                     GIEKWGFNVDYLGTSVPIQKLVDAAIEVNADAIMASTIISHDDVHYKNMQKLNDYAIE
                     KGVRDKLVLLAGGTQVTPEIAVEHGMDAGFGRGTKGVHVATAIVETKRKMRAEDE"
     gene            7636..9042
                     /locus_tag="VFD08_03405"
     CDS             7636..9042
                     /locus_tag="VFD08_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HZK10097.1"
                     /translation="MSKVLVYEIGSTTTLVNAFTDLDTENPRFVGGAQAPTTVTQGDV
                     TLGLRQALDELKSNFPEESFEEAKILASSSAAGGLKMTVHGLVEDMTVKAAKEAALGA
                     GANISFLTSGKLRRTDISKIKKISPNIILIAGGVDYGERDTAIYNAELIAALPIEVPV
                     IYAGNSENDEEIRLIFEEEGKAHLLHIVENVYPRIDQLNILPTRKVIQAVFEEHIIHA
                     KGMEKIREVVDSHVVPTPGSVMMATELISEICPDVMTIDVGGATTDVHSVTQGSEEIG
                     DILISPEPLAKRTVEGDLGLFINHENIRSIFANKELQKSTGLSEEELNYELLNYYSIP
                     ETENAKKLVKALTKKAVITSIHRHAGHLIDLYFSGGKKSAAMGKDLTKIRAIIGTGGA
                     LTQIEGGEKILLSIPDSNKGDRLLPQEGVKVYLDHWYIMASLGVMSMEHPKSALKLLL
                     ASLNLEENDICIENLEEE"
     gene            complement(9171..>9686)
                     /locus_tag="VFD08_03410"
     CDS             complement(9171..>9686)
                     /locus_tag="VFD08_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HZK10098.1"
                     /translation="NAFSKKLPEKIFFISAQELENLYPQLRPEQREEEITRLNKAVFI
                     HEIGDLLESGEKHDIRSPDYDDWSLNGDILLWSPVLDCSVELSSMGIRVDENRLLEQL
                     KKSDSMDRISQKYHQDLLNGLLPYCIGGGIGQSRICMYFLEKHHIGQVQSSVWPDKIH
                     RECEEEGICLL"
BASE COUNT         3195 a         1678 c         2136 g         2677 t
ORIGIN      
        1 gcagaaatat tcatacaaaa agatgcactt ttgggtgcat ctttttttat gaatcttttt
       61 gattaagccg ctggactatt tctaattagt cgatgctcaa tggtttttgg atcaaactta
      121 agtttggtaa aaataaaatc cataataact cctgttacaa aagcaaagat tagagttcca
      181 atccaaacct ggccgcccat taagtaacca aaaaaggtga ctactatttc aagaatccga
      241 cgagcaaagc cattggatat cttaaagcgc ctactaagaa ataccataag tgcatcccta
      301 ggtcctgctc caaaggcagc actaatgtaa aaataggttg caaaggaaat gataatcata
      361 gcaataaaga catagattaa actttgaaca aaagaagcag atatcggaac gagcccacta
      421 gaaagaataa gatcaaattg taggcctata agtatcatat ttaagattcc gccatagcct
      481 agggcttctt ttttaattac cacaaaaacc attattattg caccaacaag aataccagct
      541 tgaccgattg aaagaccagt cacattagaa aggcctacat ggaaggcttc ccaaggtgca
      601 tatccaatat gagcttgata gccaacaacc atgcccaagg aatagagaaa cagcccaaaa
      661 aacaatttaa agatatcaga atacatcttt ttcatagtaa ccctccttat taaagctact
      721 aaaccattat aggggatagt tagatcaaat aatagatgca taatattttg catctttaag
      781 aaattgatgt atacttatag aggaggagtt atgcaaaagg agattttaaa gatacttctt
      841 gaacatatgc atgaaggagt tcatgttgtt gatcaagaag gaaatagtct tatatataat
      901 gaagccttat ccaaaataga agggttactg cccgaggaag ttttaggaaa gaaaataaag
      961 gacctttttc cctatctcaa cgatgagtcg tccactttaa ttcaggccat gcatacgggc
     1021 aaaaaacagt atcaaaacct gcaagattat acaaactttc gtggggagcg aatctccact
     1081 atgaatagaa gttaccctat tataaaagag ggaaagatta taggagccat agagctgagc
     1141 aaagatgtgt ctcatcttgc tgagcttagt aaagaagtag gaaagtttaa aggaaaaaac
     1201 aaaaaaaact tcaagcccaa ttactatacc tttgatcata tagtagggga gagtccggct
     1261 attaaagagg cccttagaag ggcaaaacgc tgtgctaaaa gtgatagtac agtgctcatt
     1321 tatggagcta caggaacagg aaaagaactt tttgctcaat ccattcataa tgcctctaga
     1381 agaaaggccc acccctttgt tgctgttaac tgtggagcac tcccaggaac tttgcttgag
     1441 agcatccttt ttggcactgt tcgtggtgcc tatacgggct cggtcgatag caggggtctc
     1501 tttgaacagg ctgatggggg aacgctattt cttgatgaga tcaattccat gagtttggaa
     1561 ttacaaagta agcttttacg agttcttcaa gagagctcca ttcgaaaggt cggaggcgag
     1621 aaggagcatt atattgatgt tcgtgttatt gctgctacaa ataaagatcc ccttattcaa
     1681 gtagaagaag gaagccttag aaaagatctt tattatagac taaatgtctt gcctttatat
     1741 ttacctgaat taagagaaag ggtgggggat attgaaatac ttagcaagta ttttctagaa
     1801 aaaaaccttg aagaaaacca gcagatcaaa ggtctttcag aggaattaat aaaaaattta
     1861 caaaggcatc cattaccagg aaatgtgagg gaactagaac atttaatcaa agcggctatt
     1921 aatcttaagg atgatgatgg tgaacacttg cagattagtg atatgccaaa tagctatttt
     1981 tttgaaagag aaatgggaga actttataac ttagaggatc gaccccttca agagtattta
     2041 gaagagttgg agagaaattt agtcgcttta gccttcgcaa aagaaaatag caatgtaacc
     2101 agggcagcaa aacgtcttgg gatcagtcga cagagtttgc aatataaact taaaaaatac
     2161 agcattgagt agcaaaaagt tttgctatag gcgcaaaaac atttgcggtc ttttattata
     2221 attggttaag aaggagcatt tagcaaggtt aattagctgg cacaaacctt gctattacaa
     2281 tagtataaaa atacctggag gtaaaagatg aaagaaaacg ttcgtgtaat tatttgggga
     2341 cccggagcaa tgggcggtgg catggcaaaa atgctactta agaaaaaagg cgttgaaatt
     2401 gtaggtgcaa ttggaaaccg ttccaagggt aaagattttt accaacatat tggagcagaa
     2461 caaggtaata atccagatct tattattggt agtgccgaag atgttattaa accaggtgct
     2521 gctgatattg ttatgctagc gacggattct tttactaagg caagttttcc taaaatgaaa
     2581 acaatccttg aaaacaaaat gaattgcatt actactgctg aagaaatggc ttatcctaaa
     2641 gctcaaactc cagaacttgc ggaagaactt gatcgtttag ccaaggaaaa tggcgttaca
     2701 atcctaggta ctggtatcaa tccaggactt attatggact tactagtagt aatgtggact
     2761 ggatgtatgg aagaagtaga agaaattact tctcgcagag taaattctct gtctcccttt
     2821 ggcgagctag ttatgcatga gcagggagta ggaattactg ttgaagaatt caatgaaaga
     2881 aaagctgctg gcaaaatgac aggtcatgtt ggttttgctg agtcagttca aatgatagcc
     2941 gatgggattg gttggaaact tgataaattt gaacaagata tggaacccat tgttactgat
     3001 gttgatagaa aatcttccca tggttttgcc aaggcaggca accttgctgg agtaaatatg
     3061 acagcagaag gatatgtaga cggagttgct cgtatccata tgcaacatcc tcaacaaatt
     3121 gaaccagaac aagttggcat taatacaggt gactatgtta ctatcaaggg ctctcccaat
     3181 gtaaatatgg ctaatagtcc tgagattgaa ggcggaattg gcacttatgc aatgtgcgtt
     3241 aatatgattc cccacgttat aaattcaaga cctggcctaa aaactatgct tgatcttcca
     3301 tgtccccgcg ctattatggg tgatatgaga gatcttatcg aagaatagga gaaaagaatg
     3361 attaagaaaa acacatgggt tcgtatacat gatattgtat taaaacccga agaacgtgca
     3421 gataatattc ctgaagacac aaaagctgtg cccctagaat tttggacaaa gggctatcta
     3481 ctagaagacg ctaatatggg agatgcagtc aaggtaaaaa ccaaggctgg aagaattgtc
     3541 gaaggaaaac tggttgaagt ggacccacaa tatcaactag actatggcaa gcttgttcct
     3601 gaactcttaa gagtaggcga agatgcccgt agaatattaa gggaggggaa atgaaaaatt
     3661 ataatgattt aatggcaaga aaaaacgaaa ttatgaaaac cgctgtaggt attgactatg
     3721 atacttttga acttgaaggt tgtgcctttg attttgagcg tatgatgcgt gaaacaggat
     3781 atacaatcga agagcttaga gaaattcaag agtacttctt tgtaggccat acacctataa
     3841 ttgaaatgta taatctcaca gaacttgcaa gaaaatatgc caaacccgga tatggggcga
     3901 ggattatgat aaaagatgaa gccaccaatc cctcaggaag ctttaaagca cgccgcgcca
     3961 gcattgctgt gcaccaagcc aaaaaacttg gttataaggg gattatggct gctaccagtg
     4021 gtaattatgg agcaggggtt gcatccttag cagcaaaggt tggtcttgat tgtatcgttg
     4081 ttcaggaaac ctatgatagc aggcatgtag gtcaacctga aattgttgaa aaagctcgtg
     4141 cttgtgaagc ctatggtgcg gaagttatgc agcttactgt aggtcctgag ctcttttatg
     4201 tattcttaga tatactcgaa gatacaggct acttcaatgc ttctctttat acgccttttg
     4261 gaattgctgg tgttgaaact cttggttatg aaattagcat ggatatgaga gaaaaatacg
     4321 gcaaagatcc tgaggttgta gtggtaacca atgcaggtgg aggaaatctt acaggaacag
     4381 ctagaggcct tattaaagca ggagccacag atacaaagat tgttgctgct tctgttgatc
     4441 tttctgggct tcatatggct agtgataccg actttaatag aaagagcttt accacaggtc
     4501 atactggctt tggtattcct ttctgtacaa atccagaccg atctgatgtt cctcgttcag
     4561 ctgctagacc aatgcgatat atcgatgaat actataccat tggccaaggt agtgtctttt
     4621 atatgacaga acttcttgca aatcttgaag gtttagagcg tggtcctgca ggaaatactt
     4681 ccttagcagt cgcctttaaa ttagctcaag agatgaaaga agatgaaatc atactagtac
     4741 aagaaacaga atatactggt gctggaaaac atattaatcc tcaactaagc tttgctaggg
     4801 atcacggtat tgagatttac tttggtgaca cagaagatga agtaccaggc aaaaacatta
     4861 ttctaccaga agatccaggt aaaattgatg ttgttgaact tgatcttatg ggaatcaaaa
     4921 gatcctatat gagaaatatc cttcgtacaa aagagggcga gttttcagat gtagagatta
     4981 aatatctatg tgaggagatc aagttaaacg aagaagaact tcataaactt atttcagaac
     5041 ttagagaata gggggggtaa aatgaaaggt tttcgaagag aagacgatta tttatcgcgc
     5101 cgagaatcca tagcaaattt aagcgatgag gagctcaaag acagattttg ggacaacatc
     5161 gaaaagatcg tagatcctct tttagaccaa gcttataagt acacctcgcc ttctattgag
     5221 cgaagcatcc ttttgcgtat gggcttttct agtttagagg ctaagccttt agtagaaatg
     5281 gccatagacc atggcatgat caagcatggt gttgggcatg tagtgtatct tgttagtaaa
     5341 aaattagatc ttccaattcg tgatgctggt atgaagctta ttgaaggaga atactgggat
     5401 cttgtcaaag aagagctggg aggtaaataa tggaattaaa accaaatgag aagctagata
     5461 ttcatgagat tttaaaggat ttggaaaact acgaacccaa gcgaagagga tgggcctggc
     5521 gggaaaaagt agaagatctt gaaatggatg gacatattta taaagaatgt tccaccccat
     5581 taaagcaatc tgttggtatt cctgcggctg ttcagtactt tgataacctg gatccacaac
     5641 cccaggaatc aattacatcg gaaattgcct cagggcgatt tgaagatgat atccgtcgta
     5701 tgagaatggc agcctggcat ggtgcagacc accttatggt tattcgtact gcaggccaaa
     5761 gccactatga tggattaatt gaaggaactc cccagggtat tggtggagtt gctattacaa
     5821 gaaaacaagt tagggctcaa agaaaagctc ttgatattat tgaagaagaa gtaggaagac
     5881 ctataaatta ccattcctat gtttctggcg tggctggccc tgaaattgcc gttctttttg
     5941 cagaagaagg cgtcaacggc gcccatcagg atccccagta taatgttctt tatagaaaca
     6001 tcaacatggt tagatcctat gtcgatgctg ctgagagtaa gaaaattatg gcctgggctg
     6061 atatcaatca gattgacggt gctcataatg ccaatgcaac agctagggat gcttggaaag
     6121 ttatgcctga gcttctagtt cagcacggaa tcaattccct ctttagtgaa aaagttggaa
     6181 tgaagccaag caatatttct ctatctacag ttccaccaac agcagctccc attcctacta
     6241 tgcgttatga ccttccatac gcagtggccc tacgtcagtt ttttgataaa tataaaatgc
     6301 gtgctcagat gaatacaaag tatattactt cttcctctag ggaagcttct gtgactcatg
     6361 tgatgaatat gctgatttca agacttacca gtgctgatat tcagtccacc atcaccccag
     6421 atgagggaag aaatgttcct tggcatatgt ttaacataga agcctgtgat acagccaaac
     6481 aatctcttgt tggcatggat gggcttttag agatggttga acttcgcgag gatggatatc
     6541 tacctgccaa agtccgagaa attcaagaaa gagccgtttt atatctagaa gaaatgctag
     6601 aaatgggtgg ctactatgaa tctgttgagc gcggtatgtt tgttgactct ggtgaatatc
     6661 ctgagcgtaa tggcgatggt atagccagag aaattcaagg tggaataggt gctggtagcg
     6721 tctatgaaag agctgacaac tatatggctc ctgttacatc ccattatgga tacaataata
     6781 ccaaacaata tggtgttgaa tctgaaaatg ctgctgaatt aataggtgga tcaacctttg
     6841 aagaccgcag taagattcag tatattgatg aactagatcc tgttgataat gtaaacactc
     6901 gtttagctga aaaagaagcc tatagaaata ccaatatgat tatgcctgag gtagaatggc
     6961 tggctgatgg tattgtgcag ctagaacttc ttcttcctgt tcctatgcaa gtagcaaaat
     7021 acgcagctct tgaatacgcc aaacatatgc gtatggaaga tgctgaagta gtacatcttg
     7081 agtgtatgca gcctgctgaa ggaaccagag tacaactaaa gggtaaggtc aactttgctg
     7141 tagatataga cagccttgaa attcctcctg agccaaagat ttattctgat gatgagcttt
     7201 ggaaggaact agaggaacat cctatgaagg ttgttgcagg cacggttgga gaagatgagc
     7261 actcggtagg gcttcgtgaa atcattgata tcaaacacgg tggtatcgaa aaatggggct
     7321 ttaatgtaga ttatctagga acatcagttc caattcaaaa acttgtggat gcagccattg
     7381 aagtaaatgc tgatgccatt atggcttcaa ctattatttc tcatgatgat gtccactata
     7441 aaaatatgca aaaactaaat gactatgcca ttgaaaaggg agtaagagac aaattagttc
     7501 ttttggctgg gggaacccag gtaactcctg aaattgctgt tgaacatggc atggatgcag
     7561 gctttggccg tggaaccaag ggtgttcacg tagcaacagc cattgttgag acaaaaagaa
     7621 aaatgagagc tgaagatgag taaagttctg gtctacgaaa tcggttcaac aacaaccctt
     7681 gttaatgctt ttaccgattt agatacagaa aacccccgtt ttgttggcgg ggctcaggcg
     7741 cctacaaccg tgacccaggg ggatgttacc ctgggtcttc ggcaagccct agatgaacta
     7801 aaaagtaatt ttccagaaga atcctttgaa gaggcaaaaa ttctcgccag tagctctgct
     7861 gctggaggat tgaaaatgac agtgcacgga ctggttgagg atatgacggt aaaagctgct
     7921 aaagaagcag ctcttggagc cggtgcaaat ataagttttc ttacctcagg caaactgcga
     7981 agaacagata tttctaagat aaaaaaaatt agtccaaata ttattcttat tgcaggggga
     8041 gttgattatg gtgagcgaga tacagccata tacaatgccg agctgattgc agctcttccc
     8101 atagaagtac cagtaatata tgctggaaac tctgaaaatg atgaggagat aaggttgatt
     8161 tttgaagaag agggcaaggc ccatctactt catattgttg aaaatgtcta tcctaggatt
     8221 gaccaactaa atatcctacc aacaagaaaa gttattcagg cagtttttga agagcatatt
     8281 attcatgcta agggaatgga aaaaattaga gaagttgttg actctcatgt ggtaccaaca
     8341 ccaggttcgg ttatgatggc cacagaactc atttcagaaa tctgtcctga tgttatgaca
     8401 attgacgttg gaggggccac cacagatgtt cactctgtaa cccagggaag tgaagaaatt
     8461 ggggatattc taatatctcc tgaacccctg gccaaaagaa ccgttgaggg tgatctggga
     8521 ctttttatca atcacgaaaa tattagaagc atttttgcca ataaagagtt acaaaagtca
     8581 acaggtctta gtgaagaaga gttaaactac gagcttctta attattactc tatccctgaa
     8641 acggaaaatg caaaaaagct tgtaaaagca ttaacaaaaa aagcagttat aacttctatt
     8701 catcgtcatg ctggtcatct tattgatcta tactttagtg gcggaaaaaa gagtgctgcc
     8761 atgggcaagg atctaactaa gattagagcc attattggaa caggaggagc tcttactcaa
     8821 atagaggggg gcgaaaaaat tcttctttct atccctgatt ccaataaagg agatcgccta
     8881 cttcctcaag aaggagttaa ggtgtattta gatcattggt atattatggc cagtcttggt
     8941 gttatgagta tggagcatcc taaatcagcg ttaaaactac tcttggccag tttaaaccta
     9001 gaagaaaatg atatttgtat cgagaatcta gaagaagagt aaaaaagtaa ctttagaaaa
     9061 aaaatcctcc cagataaact agggaggatt ttttgactat aaatcgtata atttccttgg
     9121 ataaggattg ttattattta ctaaaaaacc acgaagataa agtactgttt ttaaagcaag
     9181 catatacctt cttcttcaca ttccctatgg atcttgtcgg gccatacaga agattggact
     9241 tgaccaatat ggtgtttttc taaaaagtac atacagattc gcgattggcc tattcctcca
     9301 ccgatgcagt agggtagcag tccattaaga agatcttgat ggtatttctg gctaatacgg
     9361 tccatagaat cacttttttt cagctgttca aggagcctat tttcatcaac tcgaataccc
     9421 atactagata actccacgga acaatctaag acggggctcc ataggaggat atctccattt
     9481 aagctccaat catcataatc agggcttctg atatcatgct tttctcctga ttccagaaga
     9541 tctccaattt cgtgtataaa gactgcctta tttagcctag tgatttcttc ttctctttgc
     9601 tcaggcctta gttggggata caggttttca agttcttggg ctgagataaa gaatattttt
     9661 tcgggaagtt ttttagagaa ggcatt
//
LOCUS       DASRNA010000124         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_559662,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000124 DASRNA010000000
VERSION     DASRNA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559662"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..2413
                     /locus_tag="VFD08_03415"
     CDS             479..2413
                     /locus_tag="VFD08_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HZK10099.1"
                     /translation="MKTSYRPLIILLICTLLMTTACMESAEAPEAGELEESAEEAEAD
                     QTEEPAEEAEASEEEEIKASEEEEREPLAGGLDPAVVPVNPILLEKYEEFFPPRVITV
                     TDGVYLACGYNRDNPALIEGEEGLIVIDPGESIFAAEAIKAAFNAELDNIFDKKPVEA
                     IIYTHSHDCHIHGAAVFADSQTEIIAHEDLMDSLFDEWYGQVYPSRILGGAMMAGIMY
                     QDVPAIGNEGWFAGYVLAGENIPGPSGFMKPTILVKDKLETTIAGVELNLIAAPGETR
                     DIIVIWLPEKETLIEIGLIYEAFPALTTMRGSRQRDAMDYVESLKMCRGLNPEYLVAL
                     HGPNPITEGADNIQDYLTNFSDAIQFMNDQTIHHLNKGYTAGEMMDLIELPPHLASNS
                     YLKETYGSLDWNIVHLSRYYRGYYTGQTRDLFPQSTEDEANMSVMLAGGVDELILKAE
                     EALEDGNLEWALRLSDDVLVLNPDNSQAFDTKKAAMLALAEDTMNSQARNMLLSEYIL
                     MTGQGRLPFELGDSATMYSNMSEVAVELMPIETLHRILAVNLVASDSMETDIVAGLEL
                     TGINEGEPADYTLNIRKGILEFDPPISEEAQFAISTDLMTWKHLVLHKMTAEDAIAQG
                     VVDITSGSEEDFYNFMKFFN"
     gene            complement(2566..3594)
                     /locus_tag="VFD08_03420"
     CDS             complement(2566..3594)
                     /locus_tag="VFD08_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HZK10100.1"
                     /translation="MNKIIIDKAGLLTTIQDKGRWGFQQYGISVAGAMDHFAMTLANL
                     LLGNDSNKALLECTYSGPEISFYCDEIISITGADMSPKINGKAVAMWTSLLVKAGDKL
                     QLSGASSGLRSYIAFSRELLVPALMGSKSTFMRARLGGLGGNKLSKGDEIRLGEKEAS
                     SQGTYLPPKYIPSYNKDACLRVVMGPQDDYFTKEAISTFLTSSYQISPEADRMGYRLE
                     GPRINHKDQVDIISDGIVFGSIQVPGSGAPIIMMSDRQTTGGYPKIATLITPDLSVLA
                     QMGPGMRVNFKKVSVDEAHNLYKEHINKLAEIKEALDKRHFHLGKTKNYILSLKGKSY
                     KVQVDEIL"
     gene            complement(3595..4332)
                     /gene="pxpB"
                     /locus_tag="VFD08_03425"
     CDS             complement(3595..4332)
                     /gene="pxpB"
                     /locus_tag="VFD08_03425"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HZK10101.1"
                     /translation="MNKNIRFLPAGDSSIVMEFGKIISPQINLQVQNMARAITKAQKT
                     YILETIPTYRSLLINYDPLEVSFKLLVAELSELMLTISKEEAKKIRIVEIPVFYGGDY
                     GPDLDFVAKHNKISPEEVVDIHSSRDYLVYMLGFTPGFPYLGGMDERISAPRLTTPRI
                     EIPPGSVGIAGSQTGIYPLASPGGWQLIGCTPVQLYDPLAEPPALLKAGDYVRFLPIK
                     KEEFSQIKEQVEKTHYQVNIIIEEGVS"
     gene            complement(4388..>5155)
                     /locus_tag="VFD08_03430"
     CDS             complement(4388..>5155)
                     /locus_tag="VFD08_03430"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HZK10102.1"
                     /translation="KYVVDLNSDLGESFGAYKLGADEEVLKYVSSANIACGWHAGDPM
                     IMDKTVKLAIENNVDIGAHPGYPDLMAFGRRNMDITPQEARNYLLYQLGALGAFAAAQ
                     GRKLQHIKLHGAFYNTACHKKELADAIVDAIYDADKKLIILALSGSYLLQRAKEKGLR
                     TAHEVFADRAYNADGSLVNRKLPGAMIHDKNLALKRIKQMIFEGKVSSIDGKLIPIKA
                     DSICVHGDNPEAVAFVKFIKESLIADGIEVAPLGSFK"
BASE COUNT         1530 a         1000 c         1109 g         1516 t
ORIGIN      
        1 gcccttttta gattaaaaac cggccgggta gaaagtcaaa acaggtattt gttatatata
       61 attaaccagc cataaaaaaa atggctaatc tttaaaggat attaccaatc aagatatatt
      121 tttattgtta aagttaataa aaagtgtata ataaaagata taggttaaat aaaacttatt
      181 agtaaaaaag aagagacaaa tctaacgaag aagcaaaaag tatttcgaac tatttgtaaa
      241 gataaaaagg gggtgttgct tggtaagtaa aaagaaaatc gtagaaaaaa aatagttaga
      301 gcgtgcaaaa ctaaagtgcc atttataagg tttggtagcc attactaaag gaagtttatt
      361 tagggcaact ttaccaacaa acagtataga agatacaagt gggactttac ataacaattg
      421 cgaaaccgtt ctttaaagga ctaatgatta gttaaactag tataatagga gaataaacat
      481 gaaaacttca tatcgaccgc ttatcatctt acttatttgt acactgctaa tgactaccgc
      541 ttgcatggag tcggctgagg ccccagaggc tggtgaatta gaagagtcag cagaagaggc
      601 agaggctgac cagacagaag agccagcaga agaagcagag gctagcgagg aagaagaaat
      661 aaaagctagc gaggaagagg aaagggagcc tctagcaggt ggcctagatc ctgcagtagt
      721 acctgttaac cccatattat tagaaaaata tgaggagttt ttcccacctc gagttatcac
      781 agtaaccgat ggtgtgtatt tggcctgcgg gtataaccgg gacaatccag cccttattga
      841 aggagaagag ggattgattg ttattgaccc aggagaaagc atctttgctg cagaagcaat
      901 aaaagctgct tttaatgccg aactagataa tatttttgat aaaaaacccg ttgaagccat
      961 tatctacact cacagccatg actgtcatat tcacggtgct gcggtatttg cagatagtca
     1021 aacagaaatc attgctcatg aagacctaat ggattcccta tttgatgagt ggtatggtca
     1081 agtttatccc tctaggattt tagggggcgc tatgatggct gggattatgt atcaagatgt
     1141 tccggcaatt ggcaatgagg gctggtttgc tggctacgtt ctagctggag aaaacattcc
     1201 gggaccctca ggatttatga aacctactat actggttaaa gataagctag agacaacaat
     1261 cgctggagtc gaacttaatc tgattgctgc accaggagaa acaagagata ttattgttat
     1321 ttggttgcca gaaaaagaaa cattaataga aatcggcttg atttatgaag catttccggc
     1381 tcttaccaca atgaggggta gtcgccagag ggatgctatg gactatgtag aaagtctaaa
     1441 aatgtgtaga ggtctaaatc ctgaatattt ggttgcactt catgggccta atcccatcac
     1501 agaaggtgct gataatatcc aagactatct gaccaacttt agtgatgcca tacagtttat
     1561 gaatgatcaa accatacacc acttaaataa aggttataca gctggagaga tgatggactt
     1621 aattgagcta ccacctcatc ttgcctctaa ctcttatctt aaagaaacat atggttctct
     1681 ggattggaat attgtccacc tttctcgcta ctataggggc tattatacgg gccaaacccg
     1741 ggatctgttc ccacagtcaa cagaggatga agcaaatatg tcagttatgc tagctggtgg
     1801 agttgatgaa ctaatcctta aggccgaaga ggctctagaa gatggaaatc tagagtgggc
     1861 actgagactt tcagatgatg tgctggtgct aaatccagac aatagccagg catttgatac
     1921 gaaaaaagct gctatgcttg ccctagcaga agatacaatg aattcacagg cccgtaatat
     1981 gttactctct gagtatatct tgatgacagg acagggtagg ttacctttcg agttgggtga
     2041 ttcagcaact atgtactcta atatgagtga agtagctgta gagctgatgc cgattgaaac
     2101 cttgcaccga attttggcag tcaatcttgt tgcttctgat tcaatggaaa cagacattgt
     2161 tgctggctta gaactgaccg gaatcaatga aggcgagccg gcagactaca ctctaaatat
     2221 tcgcaaggga atcttggagt ttgatccacc aataagcgag gaggcgcagt ttgctataag
     2281 caccgattta atgacatgga aacatttagt tcttcataaa atgaccgctg aagatgccat
     2341 agcgcaaggt gtggtggata ttactagtgg gtctgaagaa gacttttata atttcatgaa
     2401 gttttttaac taaataagaa gataaacttc tagattaata acggacggac tatttgatat
     2461 cctgccctag taataaagac tcttattaga aagacccatt tgttgagaaa gtcttctcag
     2521 catatgggtc tttttttgtg ttcttgctaa atggtagagg taactttata aaatttcatc
     2581 aacctggacc ttgtaggatt ttccttttaa tgaaaggata taatttttag tttttcccaa
     2641 atgaaaatga cgcttatcca aggcttcttt tatttctgct aacttattta tatgctcctt
     2701 ataaagattg tgagcctcat cgacggaaac ctttttaaag ttgaccctca taccgggccc
     2761 catttgtgcc aaaacactca gatctggtgt aataagggta gcaatttttg ggtagccccc
     2821 tgtagtttgc ctatcactca tcatgataat tggagcacca ctaccgggga cttgaataga
     2881 gccaaaaacg attccatcag atattatatc tacttggtct ttatggttga tcctggggcc
     2941 ttctagtcga tagcccatgc ggtctgcctc gggtgagatc tggtagctgg aagtaagaaa
     3001 tgtactgatg gcctctttgg taaagtaatc atcttggggg cccatcacaa cccgcaggca
     3061 ggcgtcttta ttataagagg ggatatactt tggaggcaaa taagtccctt ggctggaggc
     3121 ttctttttcc ccaagccgta tttcatcacc tttggataat ttgtttcccc ctaaaccacc
     3181 aagtctagct cgcataaagg tagatttact tcccataaga gctggcacaa gaagttcgcg
     3241 gctaaaggca atatagcttc taagcccact actagcgcca cttagttgga gtttatctcc
     3301 ggcttttact agaagagagg tccacattgc aacggctttg ccatttattt ttgggctcat
     3361 atctgcacca gtaatactga ttatctcatc acaataaaaa ctgatttcag gaccagaata
     3421 ggtgcattct agaagagcct tattgctatc atttcctagg agcaaattag caagagtcat
     3481 agcaaaatga tccatggctc ccgctacgct tataccgtac tgctgaaagc cccatctacc
     3541 cttatcttgg attgttgtta ggagtccggc tttatcaata ataattttat tcatctagct
     3601 aaccccttcc tctataatga tatttacttg gtagtgggtt ttttccacct gctcttttat
     3661 ttgggaaaac tcttcctttt tgatgggaag aaagcgaaca tagtctcctg ccttaagcag
     3721 ggctgggggc tcagcaagag ggtcataaag ctgcactggt gtgcatccaa taagctgcca
     3781 accaccaggg ctagccaggg gatagatgcc agtttgtgaa ccggcaattc ccactgatcc
     3841 tgggggtatt tctattctag gtgtggtgag cctgggtgca gaaattcttt catccatgcc
     3901 cccaagatag ggaaaacccg gggtaaaacc cagcatatat actagataat cccgtgaact
     3961 gtgaatatct accacctctt ctgggctgat cttattgtgc tttgccacaa agtctaaatc
     4021 tggtccataa tctccgccgt aaaaaacagg aatttcaact atccttattt tttttgcctc
     4081 ctctttactg attgtaagca ttagttcaga tagttctgct actaatagct taaaagacac
     4141 ctctaggggg tcataattaa taagaagaga gcgataggtg gggatggtct ctaggatgta
     4201 ggttttttgt gccttcgtga tggccctagc catattttgg acctgaaggt ttatttgggg
     4261 gctaatgatc tttccaaatt ccattacgat ggaagaatca cctgcgggta aaaatctaat
     4321 gtttttattc atagtttttt ctttctttct ttataaagag cttatatcta taagaagtga
     4381 aatttatcta cttaaagctt ccaagagggg ccacttctat gccatcagcg ataagacttt
     4441 ctttgatgaa ctttacaaag gctaccgctt caggattgtc tccgtgcacg caaatggagt
     4501 cagctttaat tgggataagc tttccgtcta tggaggagac ttttccctca aaaatcattt
     4561 gttttattct cttaagggca aggtttttat catgaatcat agctccggga agttttctat
     4621 ttaccaaaga accatctgca ttataagctc gatcggcaaa aacttcatgg gctgttctaa
     4681 gtcctttttc cttggcccgc tggagtagat agctaccact taaagctagg ataataagct
     4741 ttttatcagc atcataaata gcatcgacaa tggcatcagc cagttctttc ttgtggcagg
     4801 cagtgttata aaaagcccca tggagcttta tatgctggag ctttctaccc tgggcggcag
     4861 caaaggcacc tagggcccct agctgataaa gcaggtagtt tctggcttcc tggggagtaa
     4921 tatccatgtt tcttctgcca aaagccatta aatcagggta tcctgggtgg gcaccgatat
     4981 cgacattatt ttctattgct agttttacag ttttgtccat aatcataggg tctccagcat
     5041 gccagccgca agctatattt gcagagctta cgtatttaag gacctcttca tcggctccta
     5101 gtttatatgc gccaaagctt tcgccaagat ctgagtttaa atctactacg tattt
//
LOCUS       DASRNA010000125         9635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_631249,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000125 DASRNA010000000
VERSION     DASRNA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631249"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFD08_03435"
     CDS             complement(<1..535)
                     /locus_tag="VFD08_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZK10103.1"
                     /translation="MKKHSTKELTLLALLIAIVFVSTYLVQIPMPFSRGGLIHVGDVA
                     FMCIALLFGARMGAIAGGVGMALFDILSPYAIWAPFTLVIRLLMGWLIGTISHGKKSY
                     GRNTPRNILALLASLPLLIGGYYVAEAILYGNWIAPLQSVSGNLGQYIVGFIGSLALN
                     RILGSLATVKKYQKESEK"
     gene            complement(532..1416)
                     /locus_tag="VFD08_03440"
     CDS             complement(532..1416)
                     /locus_tag="VFD08_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005969665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK10104.1"
                     /translation="MKKKSAWGPIFLLTTALIWGLAFVFQRSSMDHIGPFTFGAFRYA
                     LSALCLLPFALGKRKKDLPFTKDPTVRWGALCGLFLFAGTSFQQTGLVYTSAGKAGFI
                     TALYIVVVPLLGGLFLKQHVSLQSWICVLIATVGFYLLSVQSSFSIELGDFIIFIGVF
                     FWAGHILVIDKGVDSMEPLLLSMVQMLVASGLSFALALIFENINLSGLRGALVPILYT
                     GIFSGSIAFTLQMYGQRYSNPTLAALILSLESVFAVGAGYLFLNEILSSREALGALII
                     FVAVVLAQIPLRKNEEFL"
     gene            1900..3156
                     /locus_tag="VFD08_03445"
     CDS             1900..3156
                     /locus_tag="VFD08_03445"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HZK10105.1"
                     /translation="MNYAKDLITFIDAAPTMFQATAEAKKRLDEAGFTELSLTKLWTL
                     KEKEGYYLSFNDSCLIAFRVGTRDLLGFHLIGAHTDSPLLKLKPAPIIHCQGYTKLNI
                     EVYGGPLLSTWFDRPLSLAGRVILDKKTPQSLPVDLKKELLIIPSLAIHLSRDKKEEI
                     NPQKELLPLFSGQELTEESLLELLAENLPCKKEEILDYELYCYPVEKGCFLGKEGEFI
                     SIGRLDNLGMCHAGLQALIQAKAGEKTQVLALFDNEEVGSQTAQGAGSGLLRNVLQKI
                     SLSRKLSQEEHLCRIYDSFMLSCDQAHGVHPNYEEKSDPTNRPKLGGGPVIKLSAKKA
                     YTSDALGVALCKKLCAENKIPYQSFVNRSDMRGGSTIGPISEGQTSIKGMDLGLAVLG
                     MHSTRELQAAKDQEYFIRLLRAFLEQ"
     gene            complement(3165..3449)
                     /locus_tag="VFD08_03450"
     CDS             complement(3165..3449)
                     /locus_tag="VFD08_03450"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HZK10106.1"
                     /translation="MALVHMIKADLYLPQVHSLKQKRAIRYSLTQRLKTMNVSVIESQ
                     DQDILQRLFLLLSYSALNESYAKDKREKILELFYQETEHIRLEEETMDLD"
     gene            complement(3452..3925)
                     /locus_tag="VFD08_03455"
     CDS             complement(3452..3925)
                     /locus_tag="VFD08_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZK10107.1"
                     /translation="MSLERAKAHLKKWGFDKKVHEFDASSATVPLAARALGTKEERIA
                     KTLSFDLGERIILIVTAGDAKIDNRKFRDFFSVKAKMLEVSRVEEEVGYDVGGVCPFG
                     VNEGVDIFLDISMKRFPTIFPACGTASSAVELSPEELEKSTYESQWIDVCKGWEE"
     gene            complement(3974..4333)
                     /locus_tag="VFD08_03460"
     CDS             complement(3974..4333)
                     /locus_tag="VFD08_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10108.1"
                     /translation="MQKKGIRGLFFLGAFFLSYLLVRYGLFFLHGMKDWPLVLGFFGF
                     LCLLLALYKDKKKMQLLCLVGYSLSFFLAKLFSSTSYDQGGGRLDNTWIIWTVLYFCF
                     CLMGYFCKDKKETRDEK"
     gene            4510..4836
                     /locus_tag="VFD08_03465"
     CDS             4510..4836
                     /locus_tag="VFD08_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZK10109.1"
                     /translation="MSELLEKKCIPCSIGDLPLGEEKIAANLKKISKDWTLSQGRLTK
                     SFAFKDFKEALDFTNKVGALAEKEGHHPVIELGWGKVTIKQWTSKIKGLHENDFILAA
                     KIDKLA"
     gene            complement(4865..5542)
                     /locus_tag="VFD08_03470"
     CDS             complement(4865..5542)
                     /locus_tag="VFD08_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK10110.1"
                     /translation="MLRQIICIDEDKCVGCGLCTKACQEGAIALVDGKARLVRDDYCD
                     GLGNCLPVCPTGAISFEEREGLAYDEKAVLARKKMEEGPAAEKKPVPLTRMQTTGLQV
                     KEASSLKQWPIKIQLAPVQAKFYEGAKLLIAADCSAFVYPDFHKDFSQGSVLLVGCPK
                     LEEGDFGKKLTAIIKENAIKEVHLLRMEVNCCGGLERALYRAMEDSKKIIPWHITTLT
                     SAGVIKK"
     gene            complement(5593..6144)
                     /locus_tag="VFD08_03475"
     CDS             complement(5593..6144)
                     /locus_tag="VFD08_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZK10111.1"
                     /translation="MNSWHDINPKFLEDGSFLAIIEITKGSKVKYELDKETGLLKLDR
                     ILYTSTHYPANYGFIPQTYAKDLDPLDVLFISSEAILPLTLVHCKPLGVIRMKDGGYD
                     DEKIIAISLDDPSFQNYRDISELPKHIFNEMMHFFEVYKALEHKETAVESLGGKEEAL
                     AIIEEAIRSYKEHFPKNKDKNKI"
     gene            complement(6158..7555)
                     /locus_tag="VFD08_03480"
     CDS             complement(6158..7555)
                     /locus_tag="VFD08_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZK10112.1"
                     /translation="MQNNFKRIRLLLIIVMIVFIAVSLYLVYFQLFQAKDLNNHSANS
                     RNYLDETSITRGKIYDERGELLAYSLEEETKRRISEYPYSFAHIIGYSSTDHGKSGLE
                     SSYNAQLIGLNEDDPLGLVKDFLGKKGRGNDLYLTINSELQSYIYSMLEGHRGAIVVM
                     DPASGALHSLVSSPSFNTNEIDSTWQNLLEREDGVFLGRGTQGLYTPGSIIKVFTATA
                     LLESDISLDYYDEGVEIIGSSEIYNFRGAVEGQISLEEALIYSTNTYFANKALRLGPE
                     KLKEVADRFYFNKAISFELANEVSYIDYEEGMEDIDLAATSYGQGKALVTPLHMAMMI
                     SAVSQEGKMMEPYLVKEVRSPSGTSLTQTRQNQISQVTEPALARQLEEMLIKVVEVNG
                     YNYLEGARIGGKTGTAENPSGNDHAWYVSFAKGTNKDLALAIVLEEEGATGGVAAAPI
                     AQRIYERARQLGLID"
     gene            complement(7545..8789)
                     /locus_tag="VFD08_03485"
     CDS             complement(7545..8789)
                     /locus_tag="VFD08_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZK10113.1"
                     /translation="MNTSSLKPSQAKWLIFFFQILSLPLIFFINTEAFGEGMISFLAG
                     LILLTLVSNLLIERGNLGDKYLLLIANMLVSIALIMIYRINPYYGERQLVFYALGLIS
                     FFAVYFFLRLTGGFFQRLSYFYYLVTAGLLLVTLLFGISSGGATNWFEIGGIQFQPSE
                     LAKVSFVFFLAAYYTGEHLFKEHKYRSYILMGLVYILIGLFFLQGELGTAAVFMGVFF
                     SVQLVFEKDKKPLILNMLLSILGLYIGYKIFGHVRIRFEIWRDPWVDIQGRGYQIVQS
                     LFALAAGGLFGKGIGLGRPNTIPLSYSDFIYPAIIEEMGIFMGIAILFLFLLLFYRGL
                     KIAMKQGQAFYRVVSLGVSMLFASQAFIVVGGVLKLIPMTGITLPFMAYGGTSMLVSF
                     ILLGVLQYTSEYLTIKEEDHAK"
     gene            complement(8779..9282)
                     /locus_tag="VFD08_03490"
     CDS             complement(8779..9282)
                     /locus_tag="VFD08_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZK10114.1"
                     /translation="MDRVFSILEKILYTEFIKNINLFTLVSSILKYVFVVIVLRFIYL
                     IVRMIYLDISSSQPGKSTGTYLRLLNQRESLSFHVENQYFLGEENTIGRERTCQIPID
                     YIYLSKVHARIFKDGDDFFLEDLDSVNGTELNGQEIRGIVPLEDKDLINFVKLQFIFM
                     KGEEDEY"
     gene            complement(9336..>9635)
                     /locus_tag="VFD08_03495"
     CDS             complement(9336..>9635)
                     /locus_tag="VFD08_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK10115.1"
                     /translation="EIGFVLNKDYWNQGLMSEAVEAVIDKFFTCYGIDFFVCGHFEGN
                     EASRRVIEKSGFNYIGNVNYKGQTGIDYNTAMYYQWNPKKEDPLKDHIKEFFKRN"
BASE COUNT         3002 a         2242 c         1793 g         2598 t
ORIGIN      
        1 atttctctga ttccttttga tattttttta ccgttgccag cgaacccaaa attcgattta
       61 gggccaggga accaataaag cccacaatgt actgaccaag attgccgcta acactttgta
      121 gcggcgcaat ccagtttcca tacaaaatcg cttctgccac atagtagccc cctataagta
      181 ggggtaaaga agcaaggagc gccaaaatgt ttctgggggt atttcttcca tagcttttct
      241 tcccatgact tatggtccca attagccagc ccataagaag acgaataacc aaggtaaagg
      301 gcgcccaaat ggcgtaggga gaaagaatat caaaaagagc catccctact cctcctgcaa
      361 tggcacccat tctggcccca aataaaaggg caatacacat aaaggccaca tcgccaacat
      421 ggataagtcc accacggcta aagggcatgg gaatctgtac caaataggta gaaacaaaaa
      481 caatggctat aagtagggcc agaagggtca gttcttttgt tgaatgtttt ttcataaaaa
      541 ctcctcattt ttgcgcagag ggatctgagc aagtacaact gccacaaaga ttattagggc
      601 tcctagggcc tctctagacg ataaaatttc gtttaggaaa agatagcccg ctcccacagc
      661 aaataccgat tcaaggctca aaattaaggc ggccaaggta ggatttgaat aacgctggcc
      721 gtacatttga agggtaaagg caatactgcc cgaaaaaata ccagtataaa gtataggtac
      781 cagggctccc cttaatccag atagattaat attttcaaaa atcaaagcca aggcaaagga
      841 caaaccagag gctaccaaca tctggaccat agaaagcaag aggggctcca tgctatcaac
      901 ccccttatca attaccaaga tatgaccggc ccagaaaaag accccaataa aaataataaa
      961 gtcccctagt tcaatactaa aactactttg cacactaaga agataaaaac caaccgtggc
     1021 aataagtaca cagatccaag actgaagaga tacatgctgt tttaaaaaca gaccccctag
     1081 aaggggtacc acaacaatgt aaagggcagt aataaaaccg gcctttccag ctgaggtata
     1141 aacaagaccc gtttgctgaa agctggtccc tgcaaaaaga aaaagaccac acagggctcc
     1201 ccaacgaacc gtcggatcct tggtaaaggg cagatctttt ttccttttac caagggcaaa
     1261 gggaagtaaa catagggccg aaagagcata gcgaaaagcc ccaaaggtaa aaggcccaat
     1321 atgatccata ctcgagcgct ggaaaacaaa agcaagcccc caaataagag ctgtagtaag
     1381 taaaaatata ggaccccagg ctgatttttt cttcaatttc tcattcctcc tttagaaaag
     1441 ttccttctaa atatgacata aaaaaagcaa aattacaaga gaggctcttt ggtcttttgg
     1501 gtagattcct gctgccaaaa gagcccttaa ctagacttgc cagaaaaaaa caaagcacat
     1561 aattatctac ttataagctt ataaaaccat ctgatccttt gccctttgtt tttaccagca
     1621 ttcctacatc tacatactat catgccttct ttttttatga aagcatatat ataaaagaag
     1681 ctctaacgtt tatccctcta aagatcccta gcatcccctt agcagatggg cttaaagccc
     1741 taaaagaggc cgctgcttac ccagtcggtc caaagctagc accctagaca tactcgcgcc
     1801 aaagagccag caaaaggcct agctggccag ctgtgaaaaa agaaaaaaac caagctatat
     1861 agtatactgt aaaaaaagct aacaatataa ggagatatca tgaattatgc aaaagaccta
     1921 attacattta ttgatgctgc cccgactatg tttcaggcca cagcagaagc aaaaaaacgt
     1981 ctcgatgagg ccggctttac tgaacttagt ctgactaaac tttggaccct aaaggaaaaa
     2041 gaaggctact atctatcctt taatgattcc tgcctgattg cctttagagt gggaacaagg
     2101 gacctcttgg gctttcacct tattggggcc catacagact cccccctctt aaagctaaag
     2161 cctgctccta ttatccactg ccagggctac accaagctca atattgaggt ctatggtgga
     2221 ccacttttat ccacctggtt tgatcggccc ctttctctgg caggaagggt tatccttgat
     2281 aaaaaaacgc cccaatccct tccggtggat cttaaaaagg aactccttat tatacctagc
     2341 ctggccattc atttatctag ggataaaaaa gaagaaataa accctcaaaa agaacttctt
     2401 cctctttttt ctgggcagga actcacagaa gaaagtctac ttgaacttct agcagaaaat
     2461 ctcccctgca aaaaagaaga aattctagac tatgaactct actgctatcc cgtggaaaag
     2521 ggttgttttc taggaaaaga aggagaattt atctccattg gaagactgga taatctaggc
     2581 atgtgtcatg ctgggctcca ggccctaatc caagccaagg caggagaaaa aacccaagtc
     2641 ctggctcttt ttgataatga agaagtaggc agtcaaacag cccaaggagc aggaagcggc
     2701 cttcttcgca atgtcctgca aaaaattagc ctaagcagaa aactttccca agaagaacat
     2761 ctctgccgaa tctatgactc ctttatgctc agctgtgatc aggcccatgg ggtccatcca
     2821 aactatgaag aaaagtccga tcctaccaat aggccaaagc ttggaggggg acccgttatc
     2881 aagctatcgg caaaaaaagc ctataccagt gacgccctag gagttgccct atgtaaaaaa
     2941 ctttgtgcag aaaacaagat cccctatcaa agctttgtca atcgctccga tatgcgaggc
     3001 ggtagtacca ttggccccat cagcgaggga caaacctcaa taaagggaat ggatctaggc
     3061 cttgctgttt tagggatgca ctccacgcgc gaactccaag ccgccaagga tcaggagtac
     3121 tttatccgcc ttcttcgcgc ctttttagag cagtaaaaat ccttctagtc caaatccatg
     3181 gtttcttcct ccagccttat atgctctgtc tcctgataaa aaagttctaa aatcttttct
     3241 cttttatcct tggcatagct ttcatttagg gcactataac taagaagaag aaaaagccgc
     3301 tgcaaaatat cctggtcctg actttcaatc acagaaacat tcatggtctt taggcgctgg
     3361 gttagggagt agcggatggc ccgcttctgc tttaaactat gaacttgagg aagatatagg
     3421 tcggccttta tcatatgtac tagggccacc tttattcctc ccagcccttg caaacatcta
     3481 tccactgact ctcataggtg gatttttcta gttcttctgg actaagttcc acagcgctac
     3541 tagccgttcc acaggcgggg aagatggtgg gaaaacgctt catacttata tcaagaaaaa
     3601 tatccacccc ctcatttacc ccaaagggac aaacccctcc aacatcgtag ccaacctctt
     3661 cttccacccg ggaaacttca agcatttttg ccttaacaga aaaaaaatct ctaaatttac
     3721 gattgtcaat ttttgcatct cctgctgtta caattaggat aatgcgctct cccaaatcaa
     3781 aacttagggt tttggcaatc cgctcttcct tggttccaag ggccctagct gctagtggca
     3841 cagtagcact agaagcatca aattcatgta cctttttatc aaagccccat ttctttaaat
     3901 gagccttagc tctttctagt gacatctttc ctcctttaca aatccttctc ctataagacc
     3961 tagtaaataa tagctatttt tcatccctgg tttctttttt gtccttgcaa aaatagccca
     4021 taaggcaaaa acaaaaatac aagacagtcc atattatcca agtattatct agcctccctc
     4081 ctccttggtc atagcttgta gaagaaaaca gtttggctag aaaaaaagac aggctatagc
     4141 caactagaca aagaagctgc attttctttt tatctttata tagggccagt aaaaggcaaa
     4201 gaaaaccaaa aaatcccaga actagaggcc agtctttcat gccatgaaga aaaaaaagac
     4261 catagcggac caaaagataa gacagaaaaa aggcgcctag aaaaaataat cctcttattc
     4321 cttttttttg cataaatagt cccccttttt ctagcccacc tatatccatt atactcaact
     4381 taggcctaag ggacaatatt tcatggtaga agcttctttc tacaaataaa gaaaatcccc
     4441 cccgcctgca tctctcatct atttatgggt aaaaggtgag taagacaaac taggcataag
     4501 gaggtagtta tgagtgaact cttagaaaaa aaatgcatcc cttgcagtat aggtgatctt
     4561 cccctagggg aagaaaaaat agcagctaat ctaaaaaaga ttagcaagga ctggaccctt
     4621 agccagggcc gacttacaaa aagctttgcc tttaaggact ttaaagaggc cctggacttt
     4681 acaaataaag taggcgccct ggcagaaaaa gaaggacacc atccagttat tgaactcggc
     4741 tggggcaagg ttactataaa acaatggacc agcaaaatca aaggccttca tgaaaatgac
     4801 tttattctag cggccaaaat agacaagcta gcataaaaca aaaagaccag ccctcgggct
     4861 ggtcttactt ttttatgact cctgcactag taagagtcgt tatatgccag ggaatgattt
     4921 tcttactatc ttccatggcc ctatacaagg cacgctccag ccccccacaa cagttgacct
     4981 ccatacgcag aagatgaact tcctttatcg cattttcttt tataatggca gtaagttttt
     5041 tgccaaaatc tccttcttct agtttaggac atccaaccag aagaaccgag ccctgggaaa
     5101 agtctttgtg aaaatcagga taaacaaagg cgctacagtc tgctgcaata agaagtttgg
     5161 ccccctcata aaactttgcc tgcacagggg ccagttgaat ctttatgggc cactgcttaa
     5221 gggaggaagc ttcttttact tgcaggccag ttgtctgcat ccttgtaaga ggtactggtt
     5281 tcttttctgc agctggacct tcttccattt tttttctggc taaaactgcc ttttcatcgt
     5341 aggcaagacc ttctctttct tcaaaactaa ttgcccctgt ggggcaaacc ggcagacagt
     5401 tgcctaaacc atcgcaataa tcatcgcgaa ctagcctggc tttgccatca actaaagcaa
     5461 tggctccttc ttggcaggct tttgtacaaa gcccacaacc aacacattta tcttcatcta
     5521 tacaaattat ttgcctaagc atattttctc cttggtatta tttgccttag tttatcatca
     5581 atcaagccca ttttatatct tatttttgtc tttattcttg ggaaaatgct ccttatagct
     5641 cctaatagct tcctctataa tcgctagagc ttcctccttg cctcctaggc tttccacggc
     5701 ggtttcctta tgctctaggg ccttataaac ctcaaaaaaa tgcatcatct cattaaaaat
     5761 atgttttggt aactctgaga tatctctata attttgaaaa ctcggatcat ccaaggatat
     5821 ggcaataatc ttctcatcat catagccccc atccttcatc ctaatgaccc ccaggggttt
     5881 acagtggacc agggttaggg gaaggatggc ttcagaagaa ataaagagaa catccaaggg
     5941 atctaggtcc ttagcatagg tctggggaat aaagccatag ttggctggat agtgggttga
     6001 agtatagagt atgcgatcga gctttaagag gcctgtttct ttatctagtt catattttac
     6061 cttgctgccc ttggttattt cgataatggc caaaaaagag ccatcctcta aaaattttgg
     6121 attaatatca tgccaactat tcattttttc cccttttcta atctattagt cccagttgtc
     6181 ttgccctttc atagatgcgc tgggcaattg gtgcagcggc tactccaccg gttgcccctt
     6241 cttcttctag cacaatggct agggcaagat ccttatttgt tcccttggca aaactcacat
     6301 accaggcgtg atcattaccc gaagggtttt ctgccgtgcc tgttttgcca ccaattcgag
     6361 ctccttcaag ataattatag ccattgacct ctactacctt gatcagcatc tcttctagct
     6421 gccttgcaag ggcaggttct gtcacctggg aaatctgatt ttgtcttgtc tgggtcaggg
     6481 atgtccctga gggactcctc acttccttta caagataagg ctccatcatt ttcccttctt
     6541 gggaaacggc gctgatcatc atagccatat gaaggggggt gaccagggcc ttgccctggc
     6601 catagctggt ggcagcaagg tctatatcct ccataccttc ttcatagtca atataggaca
     6661 cctcattggc tagctcaaaa gaaattgcct tattaaagta aaagcgatca gccacttctt
     6721 ttagtttttc cggtccaagg cgcagggcct tattggcaaa ataggtattg gtagagtata
     6781 tcaaggcctc ttccaaggaa atttgcccct caaccgcccc acggaagtta taaatttcac
     6841 tactgcctat tatttccact ccctcatcat agtagtccag tgaaatatcg ctctcaagca
     6901 gggctgtggc agtaaagacc ttgataatag accccggggt gtaaagaccc tgggtccctc
     6961 ggccaagaaa aactccatct tcacgctcaa gtagattttg ccaagtagaa tctatctcat
     7021 ttgtattaaa gctaggactt gaaacaagac tatgaagggc tccgcttgct ggatccataa
     7081 caacaatagc tcccctatgg ccctcaagca tagagtaaat atagctttgc aattcactat
     7141 ttatcgtaag ataaagatca tttccccttc ctttttttcc aagaaagtcc tttacaaggc
     7201 ccaggggatc gtcttcattt aggcctataa gctgggcatt gtaggagctt tccaggccac
     7261 ttttaccatg gtcggtggaa gaataaccaa tgatatgggc aaaactatag ggatattcac
     7321 taattcttct ttttgtctct tcttctaaac tataagcaag aagctctcct ctttcatcat
     7381 aaattttgcc cctagtaatg cttgtttcat caagatagtt tctgctattt gcgctatggt
     7441 tattaagatc cttggcctga aaaagctgaa agtagacaag atacaaactc acagcaataa
     7501 aaacaatcat cacaataata agaagaaggc ggatacgctt aaaattattt tgcatggtct
     7561 tcctccttaa tagtaagata ttcagatgtg tactggagta cccctagtag gataaaactt
     7621 accagcatag aggttccccc ataggccata aagggcaggg tgatcccagt catggggatt
     7681 agcttaagca ctccccccac cacaataaaa gcctgggagg caaagagcat agaaacaccc
     7741 aggctgacca ccctgtaaaa ggcctggcct tgtttcatgg caatttttag tccccggtaa
     7801 aaaagaagta aaaataagaa taaaatagca attcccataa aaattcccat ttcttcaata
     7861 atagcaggat aaataaagtc ggaataacta agggggatgg tattgggcct acccaggcct
     7921 atgcctttac caaagagccc tcctgctgcc aaagcaaaaa gactctgaac aatttgatag
     7981 ccccttcctt gaatatcaac ccagggatcg cgccaaatct caaaacgaat ccgaacatga
     8041 ccaaagattt tgtagcctat ataaaggccc aaaatcgata ggagcatgtt gaggataagg
     8101 ggttttttgt ctttttcaaa aaccagttgc acactaaaaa acactcccat aaagaccgcc
     8161 gctgtgccaa gctccccctg gagaaaaaac aagccaatca agatatagac aagtcccata
     8221 agaatatagg agcgatattt atgttcttta aagaggtgct cacccgtata ataggcggcc
     8281 aaaaaaaaga caaaactcac cttggcaagt tctgagggct gaaactgaat tcctcctatt
     8341 tcaaaccagt tggttgctcc tccagagctt atgccaaaaa gtagggtaac taaaagcagc
     8401 cctgctgtaa ctaggtagta gaaatagcta agcctctgaa aaaatcctcc cgttaggcgg
     8461 aggaaaaaat acaccgcaaa aaaacttatc aggcccaggg cgtaaaaaac cagctgcctc
     8521 tccccataat agggattgat gcgatagatc ataattaggg caatacttac cagcatattg
     8581 gcaattaaaa gaaggtattt atcccccaga tttcctcgct ctataagaag attactcaca
     8641 agagtaagaa gaataagtcc tgcaagaaaa gaaatcatcc cctcaccaaa ggcttctgta
     8701 ttgataaaga agataagagg aagagaaaga atttggaaaa aaaagatcag ccattttgct
     8761 tgagaaggct ttaaactact agtattcatc ctcttctccc ttcataaaga taaattgcaa
     8821 tttaacaaaa ttaatcaaat ctttatcttc cagtgggaca atgccacgaa tttcctggcc
     8881 gtttagctca gttccattta ccgaatctaa atcttctaaa aagaagtcat caccgtcttt
     8941 aaagattcgt gcatggacct tggaaagata aatatagtca atggggattt gacaggttcg
     9001 ctctcttcct atggtatttt cttcccccag aaagtactgg ttttctacat gaaaactaag
     9061 gctctctctt tgattgagca gcctaagata ggtccctgtt gattttcctg gctgagaact
     9121 gctaatatca agatatatca ttcgtacaat aagataaata aaacgcagta caatgaccac
     9181 aaaaacatac ttcaaaatac tagataccaa ggtaaataga tttatgtttt ttataaattc
     9241 cgtataaaga attttttcaa gaatgctaaa aactcggtcc atggcttcct ctttctacat
     9301 tatgtatttt ctgcctgccc tacttatacc cagaatcaat ttcttttaaa aaattctttt
     9361 atatggtcct taagggggtc ttcttttttt ggattccatt gatagtacat tgctgtatta
     9421 tagtctattc ccgtttgtcc tttatagttc acatttccta tatagttgaa gccgcttttt
     9481 tctatgacgc gcctagaagc ttcattcccc tcaaaatgtc cacagacaaa aaagtcaata
     9541 ccgtagcaag taaaaaattt atctatcaca gcctctactg cttcgctcat taggccctgg
     9601 ttccaatagt ctttatttag tacaaaacca atctc
//
LOCUS       DASRNA010000126         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_346456,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000126 DASRNA010000000
VERSION     DASRNA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346456"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VFD08_03500"
     CDS             <1..972
                     /locus_tag="VFD08_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HZK10116.1"
                     /translation="VMYGKQKDYEVQLNKMGLLSEEGFTCTCYMDEVGNIPKKGDILS
                     WAESSAVVYANSVLGARCNRNSGIIEMFGSILGKVPYFGLLTDEGRKAQWIIEVKTDN
                     LPEAQVLGSAIGLKVMENVAYIKSIDHYFDGKLDQRAKDYLKDMGAATASNGAVGLYH
                     VEGLTPEAKELAEELLVENPQVYVIDEKELKRVKDSYPIMWKDKEAKAKLCFIGCPHL
                     SLDQLISWTHRIESSLKANRQKKVLIPTVLTASTPVLKEFNSLPIADKLKGLGVETSY
                     ICPLMYMNNPLCGKMPVITNSNKLRTYTTSKYYTDSEILDAITKEGF"
     gene            973..1419
                     /locus_tag="VFD08_03505"
     CDS             973..1419
                     /locus_tag="VFD08_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HZK10117.1"
                     /translation="MKEFKGRVVVPGTTKAPALVSREGFNTLASFQKALMFGDKKGTC
                     SDQNNRDLYEKKMADKALCLPQTIGSTTGGMVLYCASALSISPSCLLFSKPIDSLAAA
                     GAILSDVWTDKNLSTIDNLGEEFLDYVQDDMIVEVLEGGIVRVKKA"
     gene            complement(1958..2158)
                     /locus_tag="VFD08_03510"
     CDS             complement(1958..2158)
                     /locus_tag="VFD08_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10118.1"
                     /translation="MASEEEKEVVKTLGNALGKSIVASIVTGIVFGAFAGIKLGFLSG
                     LLLLVSFILYGIYQVKFKKRKK"
BASE COUNT          766 a          451 c          486 g          760 t
ORIGIN      
        1 gttatgtatg gaaaacaaaa agactatgaa gttcaattaa ataaaatggg ccttctttca
       61 gaagagggtt tcacctgtac ttgttacatg gatgaggtgg gcaatattcc taaaaaggga
      121 gatatcctaa gctgggcaga gagttctgcc gtagtttatg ccaactctgt tttgggagct
      181 agatgcaata gaaattccgg tatcatagag atgtttggaa gtatcctggg caaggtccct
      241 tactttggtc ttttaaccga tgaaggtaga aaggcccagt ggattataga agtaaaaact
      301 gataatctcc cagaagccca ggttttaggc agtgctatag gcctgaaagt catggaaaat
      361 gtggcctata ttaaaagtat agaccactac tttgatggaa aattagacca aagagcaaag
      421 gactatctaa aggatatggg agctgctacg gccagtaacg gtgcagtagg cctttatcat
      481 gtagagggtc ttacaccaga agcaaaggaa ttggcagagg agcttttggt agaaaatcca
      541 caagtctatg ttattgatga aaaagagctt aaaagagtca aggatagtta tcccattatg
      601 tggaaggata aagaggcaaa ggccaagcta tgctttattg gctgccccca cctatccctg
      661 gatcagttga taagttggac ccataggata gaaagtagcc ttaaggccaa cagacaaaaa
      721 aaggtattaa ttcccaccgt attaaccgca tcaacgccgg tgctaaaaga gtttaatagc
      781 ttgcccatag cggataaatt aaaaggccta ggtgtagaaa cctcttatat ttgtcccctg
      841 atgtatatga acaatcccct ttgtggaaag atgcctgtaa ttacaaattc taataagctt
      901 aggacctata ccacttctaa atactatact gatagcgaga tattagatgc aataacaaag
      961 gagggatttt agatgaaaga atttaaagga agagtagtcg tgcccggtac tacaaaagct
     1021 cccgctctag tatctaggga aggttttaat accctggcta gttttcaaaa agccctaatg
     1081 tttggggata aaaaaggcac ctgttcagat caaaacaata gggatttgta tgaaaagaaa
     1141 atggccgaca aggccctgtg cctgccccag accattggtt ctactaccgg tggcatggtt
     1201 ctctactgtg ccagcgccct gagcatttct ccctcctgcc ttttgttttc taaacccatt
     1261 gattccttag cagcagcggg ggctatactc tccgatgtgt ggacggataa aaacttatcc
     1321 accattgata atcttggaga ggagttttta gactatgtcc aggacgatat gatagtcgag
     1381 gttctggagg gaggaatcgt tagggtaaaa aaagcctaga gcgcaaaagg aaaaaaggta
     1441 tagtttagaa gagttttttt ctaaactata cctttttaaa ctagagactt tatttaaact
     1501 tcacatggct tccctaaaga tttacctagt agtttgagcc gtccccgttc ttgtcatagt
     1561 aggcactgat gttgttaaag acaaagagta tgggtctatc tgccttatcc accactagac
     1621 tcagctacca aagacagaaa tggctttact gctttagtgt tggtatattt ttaccaaaat
     1681 agggaatgga ttgtgagtta atctagttaa ctgccataac tatttgttac atgtaacaaa
     1741 gtaatatgct tttacgtttg cacaaacctt taaaatccct gaaacaaaag ctacaagacc
     1801 atcttaagca aggttgcaaa gcaaagtgaa gcagactatg aatcaagagg ccggggtttc
     1861 aaatacctca tccgcgtcaa gtgaaaagcc cataaccatt atggttacag gctttctttt
     1921 acttttaatc tatattgttg cttgtgtttt cttaatttta cttttttctt tttttgaatt
     1981 ttacttgata tatgccatat aatataaaag aaactaataa tagtagacct gaaagaaatc
     2041 ctaactttat tccagcaaaa gctccaaata cgattcctgt tacgattgag gctactattg
     2101 atttacctaa tgcattgcct aatgttttta ctacttcttt ttcttcttct gatgccatat
     2161 tatctcctct ttttttgact ttgtatttcc tgcttttgtt aattttatcc cttggtagat
     2221 atctttgata aaaagccctt ttttttcaag gaattactat ttagcaaaag ttatttgtta
     2281 gacaaataaa tatccttcta gcctcggtct taatttcaat caattagctt agctcctgta
     2341 ttatatcact agtagctata tttttaacta aaatctaaaa cacctattgt taaaaactct
     2401 ttaactccat ggaagcaagg ggcaatcttt ctctgctttg cttgtttttt gccagtatat
     2461 cat
//
LOCUS       DASRNA010000127         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_631372,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000127 DASRNA010000000
VERSION     DASRNA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631372"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..313
                     /locus_tag="VFD08_03515"
     CDS             50..313
                     /locus_tag="VFD08_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZK10119.1"
                     /translation="MSIKPIGERVVLKEIEAEEVTRSGLILAGEKEEPQKAQIVATTE
                     KGEELGLKVGCQVYYKRYAGTKVKYENEEFIIIDNDDVLGVIV"
     gene            322..1959
                     /gene="groL"
                     /locus_tag="VFD08_03520"
     CDS             322..1959
                     /gene="groL"
                     /locus_tag="VFD08_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZK10120.1"
                     /translation="MAKDIKHALDARKSLEIGLNLLADTVKVTLGPKGRNVILERGFG
                     APLITNDGVTIAKEIELEDAFEDLGAQLVKEVATKTNDVAGDGTTTATVLSQAMVREG
                     LKNVAAGANPILIKKGIEGATQAAVDYLESISVAVDSSDDVAHVAANSAGDDKIGEII
                     SEAMQQVGRDGVITVEESKSTSTELEVVEGMQFERGYVSPYMVDDIEKKTASLEEPLI
                     LITDHKVSTNAELVPLLEQIVESGQKLLLITEDLEGEALTTLVVNKLRGTFDVVAVKA
                     PGFGDRRKDMLEDIAILTGGTYISKDLGHDLKEVSLDQLGRAKNVHITKDVTIISDGY
                     GDKEKIAARVAQIRNDIEDSDSEFDTEKLQERLAKLGGGVAVIRVGATTEMEMKERKL
                     RIEDALNATRAAVAEGILPGGGVAYVRAQKAVEDYANTLEGDYKTGALIILRALEEPL
                     RQIAANAGSESSIILEAVKVREGNIGFNALSGVYEDLFQAGIVDPTMVTRSALENAAS
                     ISSLFLTTEAAVVDLPSKEDDMPMGGMGGMGGMGGMM"
     gene            2136..>2927
                     /gene="speB"
                     /locus_tag="VFD08_03525"
     CDS             2136..>2927
                     /gene="speB"
                     /locus_tag="VFD08_03525"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZK10121.1"
                     /translation="METKELIKDPALWAGLNKPGIDIKEADVVIFGLPYDGAASFRTG
                     AKEAPRALREITYSISPTTEDLEIFSDLKVLDLGDCNCQDQETFFKEAEEMAYKCVSH
                     NKFFIMIGGDHSTTIPVHKGVDKALKEDFGIIHIDAHLDLCDSLDGNKYSHGSTQRRA
                     LELSNVKGIESIYFLGIRSIEREEIDFYQKNPLNILTAKQIRALGYIEVANRVIDKMK
                     THKNIYITVDIDGLDPAYAAGTGTPQFGGMDARELLNILEKLFKEL"
BASE COUNT          973 a          506 c          689 g          759 t
ORIGIN      
        1 tgggtatact aggatatggt tatcaataat ttaaacagga ggtatttata tgtcaattaa
       61 accaattgga gaacgtgtag tcttaaaaga aattgaagca gaagaagtca cgcgcagcgg
      121 actaattctc gcaggagaaa aagaagagcc tcaaaaggcg cagattgtag caacaacaga
      181 aaaaggcgaa gaattaggcc ttaaggttgg ttgccaggtc tattataaaa gatatgctgg
      241 aacaaaagta aaatacgaaa atgaagagtt tattatcatc gataatgatg atgtactcgg
      301 agtgattgtt taggaggaaa catggcaaaa gatattaaac atgcattaga tgcaagaaaa
      361 agtttagaaa taggtcttaa tttattagca gatacagtta aggttacctt gggtcccaag
      421 ggaagaaatg taattttaga aagaggcttt ggtgcacctc taattaccaa tgatggggtt
      481 actattgcaa aggaaattga attagaagat gcctttgaag atctaggagc ccaactggta
      541 aaagaagtag ctacaaaaac aaatgatgta gcaggagatg gaacaacaac agcaactgtt
      601 ctttcccagg caatggtaag ggaaggactt aaaaatgtgg ctgctggtgc caatcccatt
      661 ttaattaaaa aaggaattga aggagcaacc caggcagctg ttgactatct agaaagcatt
      721 tctgttgccg tagatagcag tgatgatgtt gctcatgttg cagcaaactc tgctggagat
      781 gacaaaatcg gcgagatcat cagtgaagca atgcagcagg ttggccgtga tggagttatt
      841 accgtagaag agagcaagtc tacttcaacg gaactagaag ttgtagaagg aatgcaattt
      901 gagcgtggct atgtatctcc ctatatggta gatgatatag aaaagaaaac agctagctta
      961 gaagagcccc tgattcttat taccgatcat aaggtttcta ccaatgctga acttgtgccc
     1021 ctactagagc aaattgtaga gagtgggcaa aagcttcttc tgattacaga agatcttgaa
     1081 ggggaagccc taaccactct ggtagtaaat aaactaagag gcacctttga tgtagtcgct
     1141 gttaaagccc caggctttgg cgatcgtcgc aaggatatgc tagaggatat tgctatctta
     1201 acgggtggaa catatatctc caaggatcta ggacatgatt taaaagaagt aagcctagac
     1261 caacttggac gagctaaaaa tgttcatata accaaggacg taactataat tagtgatggt
     1321 tatggtgaca aagaaaaaat tgctgccaga gtagcacaaa ttagaaatga tattgaagac
     1381 tctgatagcg aatttgatac agaaaaacta caagaacgcc tagctaaatt aggtggtggt
     1441 gtggcagtaa ttcgcgttgg tgccacaacc gaaatggaaa tgaaagaaag aaaacttcgt
     1501 atagaagatg cccttaacgc tactagagca gctgttgctg aagggattct acctggaggt
     1561 ggcgtagcct atgtacgtgc tcaaaaggcc gtagaagact atgctaacac tcttgagggt
     1621 gattataaga cgggtgcgct cattatactc cgtgccctag aagaacccct acgtcaaatt
     1681 gctgcaaatg ctggttctga gagttccatt attctagaag cagtaaaggt aagagaaggc
     1741 aatattggct ttaacgcctt aagcggtgtc tacgaagatt tattccaagc aggcattgtt
     1801 gatccaacca tggttactcg ttcagccctt gaaaatgcgg cgagtattag ttccctcttc
     1861 ctaacaacag aagctgctgt tgttgatctt ccttcaaaag aagatgatat gccaatgggt
     1921 ggcatgggtg gaatgggcgg catgggcggc atgatgtaaa taaatcagag ttctggtttc
     1981 ctaagggaag ctaggactct tttttttatc tacttccctc tatagctttc tagaaaaaac
     2041 atttggcgat atttggcgct tgatttgaat ttatgggtta gagtgcttat aatagccata
     2101 gaaagacaaa aaaataaaaa caaaggagta ggactgtgga gacaaaagaa ttaattaagg
     2161 acccggctct ttgggctgga ctaaacaagc ctgggatcga tataaaagag gcagatgtag
     2221 taatttttgg ccttccctat gatggggcag cttcctttag gaccggagcc aaagaagctc
     2281 caagggccct tcgtgaaatc acctatagca tttctcctac cacagaagat ctagagatat
     2341 tttctgactt aaaggtccta gatcttggcg attgcaactg ccaggaccaa gaaacctttt
     2401 tcaaagaagc agaagaaatg gcttataaat gtgtaagtca taataaattc tttattatga
     2461 ttgggggaga tcattccact accattcccg tccacaaggg agtggataaa gcacttaagg
     2521 aagactttgg tattattcat atcgatgccc atctggacct gtgtgatagt ctagatggaa
     2581 acaaatactc tcatggctcc acccagcggc gggcacttga gctttcaaat gttaagggaa
     2641 ttgagtccat ctattttctt ggcattcgct ctattgaaag agaagagata gacttttatc
     2701 aaaaaaaccc cctcaacata cttactgcaa aacaaattag agccttggga tatatagagg
     2761 tagcaaatag ggtcatcgat aagatgaaga cccataaaaa catttatatt acagtggata
     2821 tagatggact agatcctgcc tatgctgctg gcactggaac cccacagttt ggggggatgg
     2881 atgcaaggga actgctaaat attttagaaa aactatttaa ggaactc
//
LOCUS       DASRNA010000128         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_705450,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000128 DASRNA010000000
VERSION     DASRNA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705450"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..644)
                     /locus_tag="VFD08_03530"
     CDS             complement(162..644)
                     /locus_tag="VFD08_03530"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZK10122.1"
                     /translation="MKKDLQLLAQAMLNEIEGAEFYKLAAEKAPNQESKDSLLELAEE
                     EMRHYSYLKSLADQISENKVPTLEEVNEEDSPGIFDWGKTTGVDLQLGVSIYSVAMQM
                     ELKSMNYYLDAQKKAENESTKKLMGLLADWEKEHFNQFEKIYESYRDMWWSDMGYSPM
                     "
     gene            1180..2109
                     /locus_tag="VFD08_03535"
     CDS             1180..2109
                     /locus_tag="VFD08_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HZK10123.1"
                     /translation="MKKLIPILMILLLVACVPAAEEEVEGLHIVTASFPLYDFARELV
                     GEDARVEILLPPGSEVHSYEITPGDIKKVTGSDLFLYIGGSSDTWINNMVDASELEEK
                     QSLRLMDVVDLYKEEESQSMVEEEDDHADHEEEYDEHIWLSFENSKRMVEAIAKAMGE
                     LDLEEKDNYIKRAREYNEKIEALEDEYLSMVSSAKRKEIIVGDRFPFLYLVKSLGISY
                     DAAYPGCSAHVEENAQAIKGLIDRVEEEDIPVVFYIEMSRGTIADRIAEATGAKKRML
                     HSVQNITKEEMQAGEGYLSLMEKNLAALEEALN"
     gene            2109..2801
                     /locus_tag="VFD08_03540"
     CDS             2109..2801
                     /locus_tag="VFD08_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10124.1"
                     /translation="MLICAHHLAFYYGEEKILEEIDIDLEQGDYLVIAGPNGSGKSTL
                     IKGLVGLKKPQSGVVHYNFKDKKQGLGYMPQQHDFQRDFPASVEEIARTGIQRKKGFS
                     PFYSRAEKKEVLRTLTLLGIGNLARKSFSSLSGGQQQRVLLARALLASSELLILDEPT
                     AGLDPLVTREFYHLLATLNKKGTALIMVSHDLKLALEDANKVLYLEGGGGKLYQKNDF
                     LASDTGQKVVQK"
     gene            2798..3613
                     /locus_tag="VFD08_03545"
     CDS             2798..3613
                     /locus_tag="VFD08_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZK10125.1"
                     /translation="MTLIIEMFSYSFMVRGFIAGFIISLCAALLGAPLVLKRYSMIGD
                     GLSHVSFGAMAVATAFNWAPLSLAIPIVILASIVLLNLSEKGGLRGDAFLGLISTGSM
                     AIGVSAMSMTKGMNIDVTNYMFGSILSMTREDVYFSLILGAFILVSFILFYHEIFAVT
                     FDESFARVSGRPYKTYKMLLAVLTAITIVLGMRMMGAMLISSLILFPAFSTMQVFGRF
                     YQLIIFSALIGVTSFLIGMLLSYFYSMPTGASIVLVNMAFFILFYLIGKKKKS"
     gene            3670..>4250
                     /gene="ung"
                     /locus_tag="VFD08_03550"
     CDS             3670..>4250
                     /gene="ung"
                     /locus_tag="VFD08_03550"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZK10126.1"
                     /translation="MTKIGNDWDELLKKEFSKEYFQEIKKTLEQEYKSQEIYPPRELL
                     FEALKRTPFEKTRVVILGQDPYHGPGQSHGLAFSVQRGIPIPPSLRNIYKELYAEMGF
                     EPPSHGDLSSWADQGVLLLNTTLSVRRAQAGSHSQLGWKKFTGKILEILGESDLPRVF
                     ILWGNHARSRKNKIKKDCHLILEGPHPSPLSAYR"
BASE COUNT         1244 a          742 c          932 g         1332 t
ORIGIN      
        1 ataaaaaagc ttataaggtg gactggacag agtggttttt cttactctgt ttttttattt
       61 gctagttttt aaaggcctct actttttttc ttacaaaaaa aactgacctc cctaaagctg
      121 gtcttttctc ccgctctaga gggtcagttc actgctaatt cttacatggg ggagtatccc
      181 atatcgctcc accacatgtc gcgatagctt tcataaatct tttcaaactg attaaagtgt
      241 tctttttccc agtctgccaa aagtcccatt aactttttag tactttcatt ctcggctttc
      301 ttttgagcat caagatagta attcatgctt ttaagctcca tttgcattgc cacactataa
      361 atggagacac caagttgtag gtcaactccg gtggtcttgc cccagtcaaa aataccggga
      421 ctatcttcct cattaacttc ttctagagtt ggaactttgt tttcggaaat ttgatcagcg
      481 agtgatttca agtaactata gtgacgcatt tcttcttctg ctagttcaag taggctgtct
      541 tttgattcct ggttgggtgc tttttcagct gcaagtttgt agaactcggc tccctcaatc
      601 tcattaagca tagcttgggc cagaagttgt agatcttttt tcatgattac tcctttcctt
      661 tgtatatatt tttcataccc caaataaggg gtaaaatagc ctaataacac aagaagacta
      721 aggcacgggg gaaaaccagc aggtagtgtc cctataaaag agatctctta ccttttgctt
      781 ttcaagtttt tttaactata ggctctttat catcttagtt ctattctact agctatacta
      841 gaataaggcc ataaaaagaa agaaaaatag ggctattttc ttctaatcta ctgagtcaat
      901 tgacttaaca gaggctaatg tattaaactg ataatagtta tcagttttag cctttttagg
      961 gaaagaaaat ttggcgctct caacctaaga ccttgataga ttatcttgag gggactatac
     1021 tctaggaaaa ctttataggg ggagaaaaaa gcaaacctgg gctagtgcaa tctatgacca
     1081 gcttagagga gcatattaaa aaagcacata ctttcttact tgatgattgt gatagggtac
     1141 tttatggcaa atgcatggat tgtaaagaaa ggtagaagaa tgaaaaaact aattcctatt
     1201 ctaatgatat tattacttgt tgcttgtgtg cctgctgccg aggaagaagt agaaggactt
     1261 catattgtaa cagctagttt tcctctttat gattttgctc gagaacttgt gggagaggat
     1321 gcaagagttg aaattctact gcctccaggt tcggaagttc acagctatga gattacccca
     1381 ggagatataa aaaaagtgac tggaagcgac ttatttttat atatcggggg aagcagtgat
     1441 acatggatta acaatatggt ggatgcttca gagctagagg agaaacaaag tttacggctg
     1501 atggatgtgg tggatcttta taaagaagaa gaaagtcaga gtatggtaga agaagaggat
     1561 gatcacgcag atcatgaaga agagtatgat gagcatatat ggctctcctt tgaaaatagc
     1621 aagcgcatgg tagaagccat tgccaaagct atgggagagc ttgatctaga agaaaaggac
     1681 aattatataa aaagagcaag agaatataat gaaaagatag aggcgctgga agatgaatat
     1741 cttagcatgg tatctagcgc caaaagaaaa gaaatcattg taggagatcg atttcctttt
     1801 ttatatcttg ttaagagtct aggaatttcc tatgacgcgg cctatcctgg ttgttctgct
     1861 catgtagaag aaaatgccca agcaatcaag gggttaattg atagagtgga agaagaagat
     1921 attcctgttg ttttttatat agaaatgagc cgtggtacca ttgccgatcg tatagctgag
     1981 gctacggggg caaaaaaaag aatgcttcac tctgttcaaa atattacaaa ggaagaaatg
     2041 caggccggtg aaggttatct ttctttgatg gagaaaaatc ttgcggcctt agaggaggcg
     2101 ctgaactaat gcttatttgt gctcatcatt tggcctttta ttatggagaa gaaaaaatat
     2161 tagaagaaat agatatagat ctagagcaag gggattatct tgtgattgca ggccccaatg
     2221 gctctggtaa aagcactctt atcaagggcc tagtgggctt aaaaaaaccc caatcaggag
     2281 tggttcatta taatttcaag gataagaaac agggtcttgg ttatatgccc cagcaacatg
     2341 atttccaaag agattttcct gccagtgtag aggaaatagc ccgcactggt attcaaagaa
     2401 aaaaaggctt ttcacctttt tatagtaggg ctgagaaaaa ggaagttctt agaacactga
     2461 ctttacttgg aataggaaac cttgcaagaa aaagtttttc ctccttgtct ggaggtcagc
     2521 agcagagggt tcttttagcc agggcccttt tggcttcttc agagcttctt attcttgatg
     2581 aacccactgc gggacttgat ccccttgtta caagagagtt ttaccacctt ttagcgaccc
     2641 taaataagaa agggaccgct cttattatgg taagtcatga tctaaagctg gcactagaag
     2701 atgcaaataa agtactttat ctagagggag gcggaggaaa gttgtaccaa aaaaatgatt
     2761 ttcttgcctc tgatacagga caaaaggtgg tgcaaaaatg accctgataa ttgagatgtt
     2821 tagctatagt tttatggtaa ggggttttat cgcaggcttt attataagtc tttgtgctgc
     2881 acttttagga gccccccttg tgctaaagcg gtactctatg attggtgatg ggctttctca
     2941 tgtcagcttt ggagccatgg cagtagccac ggcctttaat tgggccccct tgtccctggc
     3001 cattcccatt gttattttgg ctagtattgt ccttttaaac ttaagtgaaa aagggggcct
     3061 taggggggac gcttttctag gtcttatttc aacaggaagc atggccattg gggttagtgc
     3121 tatgagtatg actaaaggaa tgaatattga tgtgaccaat tatatgtttg gtagtattct
     3181 ttctatgact cgagaagatg tttattttag cttgattttg ggagccttta tactggtatc
     3241 ttttattttg ttttatcatg aaatttttgc tgtaaccttt gatgagtcct ttgctagagt
     3301 gagtggcagg ccctataaga cctacaaaat gctccttgcg gtgctaacag cgataacaat
     3361 tgtccttggt atgcgtatga tgggggccat gttgatttct tctttgatcc tttttccggc
     3421 ctttagcacc atgcaggttt ttggacgatt ctaccaattg attatttttt cagccctgat
     3481 aggtgtgaca tcatttctta ttggtatgct gcttagttat ttttattcta tgcccacagg
     3541 agccagtatt gtcctggtaa atatggcttt ttttattctc ttttatctta ttggcaagaa
     3601 aaagaagtcc taatgataaa aaaagacaga atgagttttt tcttgctata ataggactaa
     3661 ggagttttta tgactaagat aggaaatgat tgggatgaac ttttaaaaaa agagttttcc
     3721 aaggagtatt ttcaagaaat aaaaaagacc ctggagcaag aatacaaatc ccaggaaatt
     3781 tatcccccaa gggagcttct ttttgaagcc ttaaaacgaa ccccctttga aaaaacaagg
     3841 gtagtgattt tagggcagga tccctatcat gggccaggcc agtctcatgg acttgctttt
     3901 tctgttcaaa ggggcattcc cattccccct tctttaagaa atatctataa agaactttat
     3961 gcagagatgg gttttgaacc ccccagtcat ggggatctaa gttcctgggc agaccagggg
     4021 gttttgcttt taaatacgac cctgagtgtt cgcagggccc aggctggaag tcatagtcag
     4081 cttggttgga agaaatttac tggaaaaatc cttgaaatcc ttggggaaag tgatctccct
     4141 cgagttttta ttctttgggg aaatcatgct cgtagtagaa agaacaagat aaaaaaggat
     4201 tgtcatctta ttcttgaagg accccatcca agccctttat cggcctatcg
//
LOCUS       DASRNA010000129         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_788843,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000129 DASRNA010000000
VERSION     DASRNA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788843"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VFD08_03555"
     CDS             complement(<1..981)
                     /locus_tag="VFD08_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK10127.1"
                     /translation="MIALQVSGAKKRFDEQILFDNLDFQVKQGEKIGLIGRNGSGKTS
                     LLRVIQGSLSLDSGQIFIGKDLRIGYLSQMPIAEEMTLLAYCMEAFEDIFLLEEEIRF
                     LQLELEKDPHNSELLHKYGEKLDLFEEKNGYAANSKVKGILRGLGFEEEDFKRKIHTL
                     SGGEERRLYLARLLATDVDVLLLDEPTNHLDLQALSWLEIYLASLPSAVVLVTHDREF
                     LDRTIDRIYEIEGGKGRWYPGNYSAYTEKKREIYLQEKRNYDNFIKKQKAEEEKLRTF
                     KERSSLNQKFSARARDREKKIERMEVVQAPLWISRDMKLRFKAARATGEDI"
     gene            1245..1862
                     /locus_tag="VFD08_03560"
     CDS             1245..1862
                     /locus_tag="VFD08_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZK10128.1"
                     /translation="MDKNISMAVIRRLPKYHRCLNDMLERGIDRISSKELSDVIGFSA
                     SQIRQDFNNFGAFGQQGYGYNVEDLLFEISEIIGLSKRYRMIIIGAGNLGGALANYGF
                     DKLGFHVDALFDNNREKIGTKIGGVEVLDVNILETYMSKNPVDIAVIATPSPIAQNIA
                     DRLSVSMVKSIWNFAPTDISVPDEVKLESVHLVDSLMTLAYLLND"
     gene            1862..>2287
                     /locus_tag="VFD08_03565"
     CDS             1862..>2287
                     /locus_tag="VFD08_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZK10129.1"
                     /translation="MAHYKTEITKWLYILLDKFFLDSAFIDKHADFSAQQYYALIRFE
                     DGMSITEFNLFYNEERKKSRAFLEDLLKLGYLEKRDDPEDHRKKLLFLTIYGKKKQKD
                     LSTQLEKRLEAILNELSVNDAKGVLKFISKLNQITVDKFK"
BASE COUNT          702 a          400 c          456 g          729 t
ORIGIN      
        1 aatatcttct cccgtagctc gggctgcttt aaagcgcagc ttcatatccc ttgaaatcca
       61 aagaggggcc tggactactt ccattcgttc aattttcttt tctcggtcac gagccctggc
      121 agaaaacttt tgatttaaag aagaacgttc tttgaaggtt cgcagctttt cttcttctgc
      181 tttttgtttt tttataaagt tatcgtagtt tcgtttttcc tgcagatata tttcccgctt
      241 tttttctgta taagcactat aatttcctgg ataccatctt ccttttcctc cttcaatttc
      301 ataaattcga tctatagtcc ggtcaagaaa ttccctatcg tgggttacaa ggaccacagc
      361 agaaggcagg ctggcaaggt atatttcaag ccaagaaagc gcctgcagat ctagatggtt
      421 ggtaggttca tctaacaaaa gaacatctac atcagtggct aaaaggcggg caagatagag
      481 tcttcgttct tctccccctg atagggtgtg aatttttctt ttaaagtctt cttcttcaaa
      541 gcctagccct cttaggatgc cctttacttt agaatttgct gcatagccat ttttttcctc
      601 aaaaagatcg agcttttctc catacttatg aagaagctcg gaattatggg ggtctttttc
      661 caattcaagt tgtaaaaagc ggatttcctc ttctaaaaga aaaatatctt caaaggcttc
      721 catgcagtag gctaaaaggg tcatttcttc tgcgatgggc atctgagaaa gatatcctat
      781 gcggaggtct ttgccaataa atatttgacc gctatcaaga gacaggctac cctggataac
      841 tcgaagcagg ctggtttttc ctgaaccatt gcgtccaatt aggccgattt tttctccctg
      901 cttgacttga aaatctaaat tatcaaaaag aatttgttca tcgaagcgtt ttttagctcc
      961 acttacttgt aatgcaatca tactactagt gtagcacgca aggcagacag aataaagttt
     1021 caatttcatg aatttaatta gcaaagttta gcagaaatag cgaatcatga tttattttgc
     1081 taatgattaa taaatattta aaaccagtcc atggagcaaa accgttgata taaagggaaa
     1141 ctttggaaga tatccaaaaa agtacagaaa aatgattgtc attatattga cattatgggt
     1201 ctactttgtt aagattagat taaactaatg agggaggtag gaaaatggat aaaaatattt
     1261 ccatggctgt gattcgacgc ctgcccaaat accatcgttg tcttaatgac atgttggaac
     1321 gagggattga tcgtatatcc agcaaagagt taagtgatgt aattggattt agcgcttcgc
     1381 agattcgcca agattttaat aattttggag cctttggaca acagggctat ggctataatg
     1441 tggaggatct tctttttgag atttctgaaa ttattggact gagtaaaagg tatcgcatga
     1501 ttattattgg ggcaggcaat ctaggaggag ccctggctaa ttatggtttt gataagctgg
     1561 gctttcatgt agatgcgctt tttgataata atcgggaaaa aatcggcaca aaaatagggg
     1621 gagtggaagt tttagatgtc aatattttag agacctatat gtctaaaaac ccagtggata
     1681 tcgcagttat tgcaacaccg tctcctattg ctcaaaatat tgctgataga ctcagtgttt
     1741 ctatggttaa gagcatatgg aattttgcac cgacagatat atctgtccca gatgaagtca
     1801 aactagaaag tgttcatcta gtggatagtt tgatgaccct ggcttacttg cttaatgact
     1861 aatggcacat tataaaacag aaataacaaa gtggctctat atacttttgg ataaattttt
     1921 tttagatagt gcctttattg ataagcatgc tgattttagt gcgcagcaat attatgccct
     1981 aatacgcttt gaagatggaa tgagcattac ggaatttaat ttattctata atgaagaaag
     2041 aaaaaaaagc cgcgcttttc ttgaggattt gctcaaatta gggtatttag aaaaaagaga
     2101 cgaccctgaa gaccatcgaa agaagctgct ttttttgacc atatatggaa aaaagaagca
     2161 aaaagacctg tccacccagc tagaaaaacg cttagaggct atactcaatg aactaagtgt
     2221 taatgatgcc aagggtgtct taaagtttat ttcaaagtta aatcagatta cagtagataa
     2281 atttaaa
//
LOCUS       DASRNA010000130         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_39770,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000130 DASRNA010000000
VERSION     DASRNA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39770"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..631
                     /locus_tag="VFD08_03570"
     CDS             416..631
                     /locus_tag="VFD08_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZK10130.1"
                     /translation="MNKGTVKWFNATKGFGFISREDGEEDVFVHFSAIVGDGYRELRE
                     GQKVTFDLEDDTRNGKGERAVNVRAEA"
     gene            1024..1239
                     /locus_tag="VFD08_03575"
     CDS             1024..1239
                     /locus_tag="VFD08_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZK10131.1"
                     /translation="MNKGTVKWFNAAKGFGFISREDGEGDVFVHFSAIEGDGYKELRE
                     GQKVTFDLEDDPRNDKGERAVNVRPED"
     gene            1363..2001
                     /gene="ispD"
                     /locus_tag="VFD08_03580"
     CDS             1363..2001
                     /gene="ispD"
                     /locus_tag="VFD08_03580"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZK10132.1"
                     /translation="MAVYAIIVAAGKSRRMGEGTNKLLLPLRGKTVIENTLDLFCQES
                     RFAGIIVVTGDPFIKELAKDRGCLLASGGDRRQDSVVSGLKLLKDEDYVFIQDGARPF
                     LNQEGLDACFEMVQEKKSFVMATQITDTLKCVEGDTVIKTLKRENHVLVQTPQGSFVG
                     LLKKAYDFVERQKASVSDDASALEMIGEKIFVVFSGEDNRKITTPEDLRYLI"
     gene            1998..2498
                     /gene="ispF"
                     /locus_tag="VFD08_03585"
     CDS             1998..2498
                     /gene="ispF"
                     /locus_tag="VFD08_03585"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZK10133.1"
                     /translation="MMRIGQGIDFHRFAKNRKLIIGGLEIDYPLGLLGHSDADVLIHS
                     LCDALLGALGLGDIGEHFKDSDPQWKDLDSRVILEKTLGLLEKRRYHILNMDLTIIGE
                     EPKFSPYKEDIKENLSCLCKLPKELINIKATTTERMGALGNKEGLGAVTIVLIEKKRE
                     DNDPKL"
     gene            2482..2733
                     /locus_tag="VFD08_03590"
     CDS             2482..2733
                     /locus_tag="VFD08_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HZK10134.1"
                     /translation="MTQNYKFNPHPKCEYFPCHKGVPEKEFNCLFCYCPLYLLGKDCG
                     GNFSYHDGIKDCSACTKAHDKDSYDFIMSKMHLIFRRDD"
     gene            2733..>3469
                     /gene="gltX"
                     /locus_tag="VFD08_03595"
     CDS             2733..>3469
                     /gene="gltX"
                     /locus_tag="VFD08_03595"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZK10135.1"
                     /translation="MVRVRFAPSPTGYVHVGSLRTALYCYLFAHHHKGKYVLRIEDTD
                     QSRLVDDAVENLLQEMEWTGIIHDEGPCHVEGKIKEIGDYGPYTQSHRLEIYKKYIQQ
                     LVDEKKAYACFCSRERLDELRAAGHHGYDGYCRDLPLEEVEKRVEAGQEYVIRLKMPR
                     NEDLVFDDLVRGRISINSDDSDDQVIQKSDGFPTYHLAVVVDDHLMGITHVIRGEEWL
                     SSVPKHLVLYHYFGWERPYFAHLPNIL"
BASE COUNT         1133 a          528 c          772 g         1036 t
ORIGIN      
        1 aacgggtatt aaatactatc tggtgaataa tccaggctag tttgtaattt tttgctttct
       61 tatataaacc ctttgagtta ctatttgaag caaaaaataa aaaaggtcca cttgcctgtg
      121 taaaggctat tgtaaaaaat ataatttagt tttcaggtct ttgctatttt tgaaaagagg
      181 cggaagcctt taatggcaag acttataagg ctactaggtc ataaaaaagc cgaaatagga
      241 tgataaaaat aggttaaatg taaaaacaat gaaaaataaa gctttacaaa caataaagcg
      301 tgctgtacaa ttaaattata tttcgatgta ccaatacggc ctatttttac atcaatatgt
      361 aaaaatgggc ttttttagtg tcatggaagt aaatatattt taggaggttt ctttcatgaa
      421 taaaggaaca gtaaaatggt ttaacgctac aaaaggattt ggttttattt caagagaaga
      481 tggtgaggaa gatgtatttg tacacttctc cgctattgta ggcgacggat atagagaact
      541 tcgcgaaggt caaaaagtaa catttgatct cgaagatgac actagaaacg gtaaaggcga
      601 acgcgccgta aacgtacgcg cagaagctta atcataaaga acaaaagggg tttgctcatt
      661 aaagagcagg ccttttttta agcttatctt ttttaagggg ggtctaccta tctagatagt
      721 gcatcagtgc tattgtaaag aagaaaaggc ctaaattcaa aactttttta atgataaaaa
      781 aagctatctt tgctatagat atttcctcca agctaggttg aacacttgga tagcagctgt
      841 ccagtttact ttgggaaaca aactttattt agaaataagc cagatagggc tttacaaatt
      901 taaaatcata gggtacaata gaatcatatt tcgatgtacc aaatattgcc tatttttaca
      961 tcagatgtag aagtaggctt tttaagtgtc atggaagtaa atatatttta ggaggtttct
     1021 ttcatgaata aaggaacagt aaaatggttt aacgcagcta aaggatttgg ttttatctca
     1081 agagaagatg gtgaaggaga tgtattcgta catttttccg ctattgaagg cgacggatat
     1141 aaagaacttc gtgaaggtca aaaagtaaca tttgatctcg aagatgatcc tagaaacgat
     1201 aaaggcgagc gcgcagtaaa cgtacgccct gaagactaat cataaagtaa aaaaaaggtt
     1261 tgctcattta tgagcaggct ttttttttac cttttttacc ttatcgactt tagtagacta
     1321 gctgtggtat gattaaacgg agaatagaac gaggtgatta ctatggctgt ttatgctatt
     1381 attgttgctg caggaaaaag caggcgcatg ggagaaggta ctaataagct tcttcttcct
     1441 ttgcgtggga aaacagtaat agaaaacacc ttggatctct tttgccagga aagcagattt
     1501 gctggcatca ttgtggttac aggggacccc ttcattaaag agcttgcaaa agacagagga
     1561 tgtcttctag cttctggtgg tgacaggcga caggattcag tggttagtgg tttgaagctt
     1621 cttaaggatg aagactatgt ttttattcaa gatggagcaa ggccgttttt aaaccaagaa
     1681 gggttagatg cttgttttga aatggtccaa gaaaagaaaa gttttgtaat ggcaacccaa
     1741 atcacagaca cattaaaatg tgttgagggg gataccgtta ttaagacgct aaaaagagaa
     1801 aaccatgtat tggtccaaac acctcaaggt tcctttgttg gtttgctcaa aaaagcttat
     1861 gattttgtag aaagacaaaa agctagtgtt agtgacgatg cttctgcttt agagatgatt
     1921 ggtgaaaaaa tatttgtggt tttttctggt gaagacaatc ggaaaatcac cacaccagag
     1981 gatttaaggt atcttatatg atgcgaattg gtcagggtat tgatttccat cgttttgcaa
     2041 aaaatagaaa gcttattata ggaggcctag agattgatta tcccttggga cttttaggcc
     2101 acagcgatgc tgatgtacta atacacagcc tttgtgacgc tcttttagga gccctaggcc
     2161 taggagatat tggggagcat ttcaaggatt cagaccccca gtggaaagat cttgacagta
     2221 gggttattct tgaaaaaacc ctgggtcttc ttgaaaaaag aagatatcat attctaaata
     2281 tggatcttac tattattgga gaagaaccaa aattctcccc ctataaagaa gatattaagg
     2341 agaacttgag ctgtctttgt aaattaccca aggagctaat taatattaag gcaacgacca
     2401 cagaaagaat gggtgctttg ggtaacaagg aaggcttagg ggcagtgact attgttttaa
     2461 tagaaaaaaa gagagaagat aatgacccaa aactataaat ttaaccccca tccaaagtgt
     2521 gaatattttc cttgccataa gggtgttcca gaaaaagagt ttaattgcct tttttgctat
     2581 tgtcctcttt atcttctggg aaaagattgt ggtggaaact tcagctatca tgatgggata
     2641 aaagattgtt cggcttgtac aaaagctcat gataaggata gctatgattt tattatgagt
     2701 aagatgcatc tgatatttag gagggatgac tagtggtacg tgtacgcttt gcaccaagcc
     2761 caacgggcta tgttcatgtg gggagtctta gaactgccct ctattgttat ttgtttgccc
     2821 atcatcataa gggcaaatat gttcttcgta ttgaagatac agatcaatca agacttgttg
     2881 atgatgctgt agaaaatctt cttcaagaaa tggaatggac cggcattatt catgatgagg
     2941 ggccctgcca tgttgagggt aaaataaaag aaattggaga ctatggccct tatactcaaa
     3001 gccatcgttt agaaatttat aagaaataca tccagcaact tgtggatgaa aagaaggctt
     3061 atgcttgttt ttgttccagg gagcggcttg atgagctaag agcagcaggt catcatggat
     3121 atgatggcta ttgtagggat ctgcctttag aagaagtaga aaaaagagtt gaagcaggcc
     3181 aggagtatgt aattaggcta aagatgcccc gaaatgaaga cttggttttt gatgaccttg
     3241 tgcgggggag aatctccata aattccgatg atagcgacga tcaggtgatt caaaaatccg
     3301 acggttttcc tacctaccat cttgcggtgg tagtagatga ccatcttatg ggcataaccc
     3361 acgtaatacg aggagaagag tggcttagtt cagtgccaaa acatctagtt ctctatcatt
     3421 actttggttg ggaaagaccg tattttgctc acttacccaa tattttaag
//
LOCUS       DASRNA010000131         5710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_788983,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000131 DASRNA010000000
VERSION     DASRNA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788983"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VFD08_03600"
     CDS             <1..1349
                     /locus_tag="VFD08_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10136.1"
                     /translation="FEEGILALIGGLGEFLDGVGDLGEGSEEFVEGLLLYLEAIDSFI
                     DYIEENSEEIEEIVKGAQDLIRALEEADISLEDLVELRGALDDLSGLLDPELFDIVEG
                     EARLDSVIAGLDGSISDLSLRIDSLRAIQESLLTSQYPFSEGEDTGLAREISDYYRMA
                     MEVEGKNLYKEISELEKIQTDLSMQKAALLLFKADYLRVKENITNLQNAIGGLNLLLD
                     NIDLGDAVDLGKLSEGLKKIIEESEGLLDTSNGLTDGLGQLEDFIAGGDQLGEGARAL
                     REGIKKLIEGAQGFEGFTGDLEAGVKELSEGAKTLFTRYQEFDAGIRSLASGLRDFND
                     GLIQYVQGFWTLHQGLQDLYIGGLELGEGTSQMAKETKDIDKKMEEEIKAKIAEMSAE
                     GIEFESFLSPKNKDISSVQFVLLYEGKTLPIDDNSNNDAEEKNFWERFLDLFKKTE"
     gene            1451..1654
                     /gene="rpmE"
                     /locus_tag="VFD08_03605"
     CDS             1451..1654
                     /gene="rpmE"
                     /locus_tag="VFD08_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZK10137.1"
                     /translation="MKKDLHPEYKDVQVRCACGNTFTTKSTRDEIRIEICSECHPFFT
                     GTQKVHDRGGRAEKFKKKYGIED"
     gene            1721..2299
                     /locus_tag="VFD08_03610"
     CDS             1721..2299
                     /locus_tag="VFD08_03610"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZK10138.1"
                     /translation="MAKLYFRYGAMNSGKSTALIQVAHNYKERGMEVLVLKPLLDTKE
                     GKLISSRIGIGKKADYLVDDSMDLFLLIKGLRAPSCVLVDEVQFFSPEQIDQLFRVAV
                     EVDIPVICYGLRTDFSMRGFPASGRLLLLAHSIEELKTICRCGKKAIFNARKVAGEFV
                     FEGDQVVIDNQADVAYEALCGQCYLKYRRKYE"
     gene            2292..3179
                     /locus_tag="VFD08_03615"
     CDS             2292..3179
                     /locus_tag="VFD08_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZK10139.1"
                     /translation="MSRCSSVGGQALIEGVMMKGDKATTMVLRKEDGLLEEIILEDPA
                     PAQGIWRYPVIRGIYSLFAAMKIGIKALDRSAQVFGEEESSFDLWLKDKFGDKAEGIV
                     LGLTILASLLLAFSLFAILPTFFISFFKGRVTNPVLLSLMEGMVKMILLIIYIATIGR
                     QKDIHRVFQYHGAEHKAVFTYESGQPLTVENARKFSRFHPRCGTSYIVFVFLISVLFF
                     SFISWTSVAMRILLKLLFLPLLAGISFEALKFTARESSLVVFLRKPGLWLQRLTTAEP
                     EDDQLEVALKALELSVHES"
     gene            3169..3954
                     /gene="prmC"
                     /locus_tag="VFD08_03620"
     CDS             3169..3954
                     /gene="prmC"
                     /locus_tag="VFD08_03620"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZK10140.1"
                     /translation="MKARELYHSLDFLGKTQQNWAFSHLVGKDYGEVFLDNPEVKGIK
                     EKLLQIKRGYKKGIPLAYLLEESYFYGRRFYVNPDVLIPRPETEGLVERALDLPWKKA
                     LDLCTGSGVIATTLYLEKGGHIQGVDLSHKALALAKKNAKELGAQVDFSQGDLFSGLS
                     GKFDLIISNPPYIKTASMMDLEVSLYEPHLALDGGRDGLDLIRRILEEAPSYLNPGGH
                     LLLEIGDEQADKLLCLGQSFCGRIEVDLAKKPRYYIGRKKEIC"
     gene            3951..5012
                     /gene="prfA"
                     /locus_tag="VFD08_03625"
     CDS             3951..5012
                     /gene="prfA"
                     /locus_tag="VFD08_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZK10141.1"
                     /translation="MREKLDKLLVKYEELSKLIGDPHVMEDMQTWQKYVKEHSDLMPI
                     VDKYTQVKRLSEELEGANELFESSDDEMKELARLEIDELEENIQALEEEIKLLLIPKD
                     PNDKKDVIVEIRAGAGGDEAGLFAGELMRMYMRYAEDHRWKAEIIDISETNVGGIKEA
                     SMQIKGKGAYSKLKFESGTHRVQRVPETESGGRIHTSTATVAVLPEADEVDVEIHNND
                     LRIDVYRSSGHGGQSVNTSDSAVRITHLPSGMVVTCQDGKSQQMNKEQALRVLRARLY
                     DKMRSEQSAAIAQERKMQIGTGDRSAKIRTYNYPQSRITDHRINFTVYQLESFLMGDI
                     DEMVEALATNYQAQLLQES"
     gene            5012..>5710
                     /locus_tag="VFD08_03630"
     CDS             5012..>5710
                     /locus_tag="VFD08_03630"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZK10142.1"
                     /translation="MTKIFEQGQIKEAAAILKRGGTVIFPTETVYGLGARYDDEKALE
                     KIFIAKGRPRDNPLILHIYTREQVARLVEEVPEKASLLMKRFWPGPLTLICKKRPEIS
                     STISGGLDTVAIRMPANEVALDLLKEVGLPLAAPSANLSGRPSPTSQEHLDDMLGRVD
                     AIVYGGDTAVGIESTVVDMTKEPPLLLRPGKVSLEELEEVVGEIGLGINKNENYASPG
                     LKYRHYAPETPLVLL"
BASE COUNT         1721 a         1099 c         1427 g         1463 t
ORIGIN      
        1 aatttgaaga gggcattctt gcccttatag gaggcctggg tgaattttta gatggagtgg
       61 gagacctggg tgaaggaagc gaagaatttg tagaaggcct tcttctctat cttgaggcca
      121 ttgacagctt tattgattac attgaggaaa attctgaaga aatagaggaa atcgtcaagg
      181 gagcccagga tttaatccgc gccctggaag aagcagatat ttctctagaa gacttagtcg
      241 agctaagagg ggctctggat gatttatccg gcctgctaga tcctgaactt tttgatatag
      301 ttgaaggaga agcaagacta gatagtgtga ttgctggcct agatggcagt attagtgact
      361 taagtcttag gattgatagt ctaagagcca ttcaagagag tcttcttact agccagtatc
      421 ctttttctga gggagaagat acaggcctgg cccgggagat aagtgattac tataggatgg
      481 ccatggaagt ggaaggaaaa aacctttata aagaaataag tgaactggag aaaatccaaa
      541 ccgatctaag catgcaaaaa gcagccctac tcctttttaa agcggattat ctgcgggtca
      601 aggaaaatat cactaaccta caaaatgcca taggagggct taatctactg ctagataata
      661 tagacctggg tgatgcggtg gatcttggaa agcttagtga aggccttaag aaaataatag
      721 aagaaagtga aggcctctta gatacttcaa atggcttaac tgatggccta ggacagctgg
      781 aggactttat agctggcgga gaccagctag gtgaaggcgc tagggcacta agagaaggaa
      841 ttaaaaaact tattgaagga gcccagggct ttgagggctt tacaggagac ctagaagcag
      901 gggtaaaaga acttagcgag ggcgctaaga cactttttac aaggtatcag gagttcgatg
      961 caggtataag aagtctggcc tccggtttaa gagactttaa tgatggcctg atccagtatg
     1021 tccaaggatt ttggaccctc caccagggcc tgcaggatct atatattggg ggactggagc
     1081 taggagaggg aacctctcaa atggccaagg agaccaagga tatagacaaa aagatggaag
     1141 aagaaataaa ggccaagatt gcagaaatgt cggcagaggg tatagaattt gaatccttct
     1201 taagcccaaa aaacaaggat atttcctcag tacaatttgt gctcttgtat gagggaaaaa
     1261 ccttaccaat agatgataat agtaataatg atgccgagga aaaaaacttt tgggaacggt
     1321 ttttggattt gttcaaaaaa acagagtaaa aacgctaaac taattacaaa atttctttag
     1381 gaatggtttg cactttttaa attcaagtgg tatactttaa aaggtatata ttaaatagga
     1441 ggtgaatgcg atgaaaaaag atcttcatcc tgaatataaa gacgttcaag ttagatgcgc
     1501 ttgtggaaat acttttacaa caaagtctac acgagatgaa attcggattg aaatctgctc
     1561 agaatgccat cctttcttca caggaactca gaaagttcat gatcgtggcg gaagagccga
     1621 aaagttcaag aagaaatatg ggatagaaga ttagagaagt ctctaatctt tttttattta
     1681 tttatataag taaagggtat aagcttataa ggaggcctaa atggcaaaac tatattttcg
     1741 ctatggagcc atgaattctg gcaaatccac agcactaata caagtggcac acaattataa
     1801 agagcggggc atggaggttc ttgtcttaaa accccttctt gataccaaag aaggcaagct
     1861 tatttctagc cgaattggca taggaaaaaa agcggattat ttagttgacg actctatgga
     1921 ccttttttta ctgattaaag gccttagggc cccctcctgt gttcttgttg atgaggttca
     1981 gttttttagt ccggagcaga ttgaccagct ttttagggtg gcagtagaag ttgatattcc
     2041 cgtaatttgt tatggcctgc gtacagactt ttccatgaga ggctttcccg ccagtgggcg
     2101 acttcttctt cttgcccact ctatagaaga acttaaaacc atttgtcgct gtggcaaaaa
     2161 ggccatcttt aatgcaagaa aggttgctgg tgaatttgtc tttgaaggag accaagtggt
     2221 gattgataac caggcggatg tcgcctatga ggccctatgt ggccagtgtt atttgaaata
     2281 tcggaggaaa tatgagtaga tgctctagtg tagggggtca agcccttatt gaaggtgtaa
     2341 tgatgaaggg ggacaaggct accaccatgg tccttagaaa agaagatggc ctattagagg
     2401 agattatcct ggaagatcca gccccagccc aggggatttg gcgctatcct gtcattaggg
     2461 ggatctattc cctatttgct gccatgaaaa taggcatcaa ggccctggac cgctcggccc
     2521 aggtctttgg agaagaggaa agctcctttg atctttggtt aaaggataag tttggagata
     2581 aggcggaagg tattgtactt ggcctgacca ttttggccag cctgcttttg gcctttagtc
     2641 tttttgccat cctgcccacc tttttcatta gtttttttaa aggcagggtg acaaaccccg
     2701 ttcttttaag tttgatggaa ggaatggtca agatgattct tcttattatc tatatagcca
     2761 ccattggaag acaaaaggat atccataggg tctttcaata ccatggtgca gaacataagg
     2821 cggttttcac ctatgaatcg ggtcagcccc ttacggtgga aaatgcaaga aagttttccc
     2881 gctttcaccc ccgctgcgga accagctata ttgtttttgt ctttttaatt agtgtgttgt
     2941 ttttttcctt tattagctgg acctcggtgg ctatgaggat cctattaaag ctacttttct
     3001 tacccctttt agcgggaatt agttttgagg ccctaaagtt taccgcaaga gaaagtagtc
     3061 tggtggtttt tttaagaaaa cctggtcttt ggctccagcg cctaacaacg gctgagccag
     3121 aggatgacca gctagaagta gcacttaagg ctctggagct atcagtccat gaaagctaga
     3181 gagctctatc atagtctgga ctttctagga aaaacccagc agaactgggc tttttcccat
     3241 ttggtgggaa aagactatgg agaagttttt ttggacaatc cagaagttaa ggggatcaaa
     3301 gaaaaacttc tacaaataaa aagaggctat aaaaagggaa ttcccctggc ctatctatta
     3361 gaagaaagct acttttacgg aaggcgcttt tatgtaaacc ccgatgtgct aattcccaga
     3421 ccagaaaccg agggcctggt tgaaagggcc ctggaccttc cttggaaaaa ggccctggat
     3481 ttatgtacgg gcagtggcgt tattgccacc accctttatc ttgaaaaagg aggccatatc
     3541 cagggggtcg atttaagcca taaggccctg gccctggcca aaaaaaatgc taaggagctg
     3601 ggggcccagg tggactttag ccagggagat cttttttctg gcctatcagg aaaatttgac
     3661 ctaattattt ctaatccccc ctatattaag acggctagta tgatggacct agaggtcagc
     3721 ctctatgagc cccatctggc cctagatggg ggaagagatg gactagacct aataagaagg
     3781 attctggaag aagccccctc ctacctaaat cccggaggtc acctgctgct tgaaataggc
     3841 gatgagcagg cagataagct actttgtctt ggacagagtt tttgtggtag aattgaagta
     3901 gatctagcaa aaaagccaag atactatatt ggaagaaaga aggaaatatg ctgagagaaa
     3961 aattagataa actccttgtt aaatatgagg aactctcaaa acttattggg gacccccatg
     4021 ttatggaaga tatgcaaaca tggcaaaaat atgtaaaaga acatagtgat cttatgccaa
     4081 ttgttgacaa atacacccag gtaaaaaggt taagtgaaga gcttgaagga gcaaatgagc
     4141 tttttgaaag tagcgatgat gagatgaagg agctggccag actcgaaatc gatgagctgg
     4201 aggaaaacat ccaggcccta gaagaagaaa tcaagcttct tcttatcccc aaggacccca
     4261 atgataaaaa agatgtaatc gtagaaatca gggcgggagc cggtggggat gaggcgggtc
     4321 tatttgcagg agagcttatg cgtatgtata tgcgctacgc agaagaccac cgctggaagg
     4381 cagaaattat tgatatttca gaaaccaatg tggggggcat aaaagaagcc tccatgcaga
     4441 tcaagggcaa gggcgcctat tcaaaactta aatttgaatc cggtacccat agggtccagc
     4501 gggttccaga aacagagtcc ggcggacgaa tccacacctc cacggccacg gtggcagttt
     4561 tacctgaagc cgatgaagtg gatgtggaga ttcataataa tgatcttcgc attgatgtat
     4621 accgctcatc tggtcacggg ggacagtcgg ttaacacctc tgactcggcg gtaagaatta
     4681 cccatctgcc ctcgggaatg gtggttacct gccaagatgg aaaaagccag caaatgaata
     4741 aggagcaggc cctaagagtc cttcgggctc ggctctacga taaaatgcgc tcagaacaaa
     4801 gcgctgccat tgcccaggag cgaaaaatgc aaattggcac aggagaccgc tcggcaaaga
     4861 tccggaccta caattatccc caaagtcgaa ttaccgatca tcggatcaac tttacggttt
     4921 atcagttaga gagttttctc atgggagata ttgatgaaat ggtagaggct cttgccacaa
     4981 attatcaagc ccaacttctt caggagagct agtgacaaag atttttgaac agggacaaat
     5041 aaaagaagca gctgctatct taaaaagagg ggggacggtt atctttccca cagaaaccgt
     5101 ttatggtcta ggagcccgct atgacgatga aaaggcctta gaaaagatct ttatcgccaa
     5161 ggggcgccca agggacaacc cccttatttt gcatatctac actagggagc aagtagctag
     5221 gctggtggag gaggtcccag aaaaggccag cttacttatg aagcgttttt ggccaggccc
     5281 ccttaccctg atatgtaaaa aaagacccga gatttcttct accattagtg ggggtcttga
     5341 tacagtggca atacgcatgc ctgctaatga ggtggcctta gacctattaa aggaagtagg
     5401 cctgccccta gcagccccca gtgccaatct atcaggcaga ccctccccaa ccagccagga
     5461 gcatttggat gatatgttag gtagggtgga tgccattgtc tatgggggag acaccgctgt
     5521 aggaattgaa tctacggtgg tggatatgac caaggagcct ccccttcttc ttcgcccagg
     5581 caaggttagc ctagaagagc tagaagaagt ggtgggagag attggtcttg gtataaataa
     5641 aaatgaaaat tacgcctctc ctggactgaa atatcgccac tatgcaccag agacccctct
     5701 tgttcttctt
//
LOCUS       DASRNA010000132         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_706859,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000132 DASRNA010000000
VERSION     DASRNA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706859"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /gene="tyrS"
                     /locus_tag="VFD08_03635"
     CDS             complement(<1..1106)
                     /gene="tyrS"
                     /locus_tag="VFD08_03635"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZK10143.1"
                     /translation="MKIYDELVRRGYISQSTHEEELRNLLEKEKVSFYIGIDATADSL
                     HAGHFLTLVVMKRMQEAGHRPILLLGGGTTMIGDPSGRDDMRQILTREDIDHNAAKIK
                     EQVSRFIDFEEGKALMLNNADWLVDLNYIEFLRDYGPYFSINRMIKMDSYQSRLEQGL
                     TYFEFSYMPLQAYDFLHLYRSEGCRLQMGGSDQWSNIIGGIDLIRGVEKVDCYGLTFN
                     LITTSAGVKMGKTQKGALWLDKEKTSPYEFFQYWRNVEDASVETLLMRYTFLEMDKIS
                     ELLAPDKNINKAKEILAYELTQLVHGKEEADRALEASKALFSGAGNLDDMPSSTLKQE
                     WLNQELLDILLELDFIKSKAEGRRLMKQKGLRLN"
     gene            1272..1730
                     /locus_tag="VFD08_03640"
     CDS             1272..1730
                     /locus_tag="VFD08_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZK10144.1"
                     /translation="MNWLLLNPVYFWLVMGGIFLIVEGVTYALVTIWFVGGALVALLL
                     AYLGVAFGFQFAAFIIVSFALLLITRPLVKRKSENITHTNVDAVLGQTGIVTKELSEH
                     FSGQGKVGGQIWTIASLDGQCIDVGKEFVVLSVEGVTLVVEPLEEEVCSK"
     gene            1718..2608
                     /locus_tag="VFD08_03645"
     CDS             1718..2608
                     /locus_tag="VFD08_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZK10145.1"
                     /translation="MFQIIPIAIISIIVLYIIVSNIRIIPQSQAYVIERLGAFQQTWG
                     TGLHIKIPFFERIAARVSLKERVVDFEPQPVITKDNVTMMIDTVVYYQITDPKLYAYG
                     VENPLSAIENLTATTLRNIIGDLELDESLTSRDIINTQMRSILDEATDPWGIKVNRVE
                     LKNILPPKDIQDAMEKQMRAERERRESILRAEGQKRSAILIAEGNKESQILEAEAYRN
                     ARIKRAEGDAQGIVLVQKAYAEGLEILMSKVPSDVFIPLKGFEALEKIADTPSSKLII
                     PSDLQGLAGMAASTAEFLKK"
     gene            complement(2682..3476)
                     /locus_tag="VFD08_03650"
     CDS             complement(2682..3476)
                     /locus_tag="VFD08_03650"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HZK10146.1"
                     /translation="MKVFISADMEGITGVTAWDETDLNHKDHIKAARQMTREVAAAAQ
                     AAIDLGYEVIVKDAHDSARNILINELPRGVRVIRGWTACPKAMMAGLDESFDAVVFIG
                     YHTSGGKDGNPLAHSFSYSKIFTCSINGKIASEFSFNRQLANYYGVPCVFISGDQEVC
                     NEARAAILGIETLAVKEAEGKATLDMHPEDALEKIYEGVTEGLKKKDQLIQKEPEYYH
                     VSFQFRLHDLAHRASFYPGVQRVSPNEIVFEGKDIMEVLTTKMFIV"
BASE COUNT         1118 a          789 c          674 g         1188 t
ORIGIN      
        1 tcattaagcc ttagaccctt ttgtttcatt aaacgtcttc cttctgcttt gcttttaata
       61 aagtcaagct ctagaagaat atctaataat tcctgattta gccactcctg ctttaaggta
      121 gaagaaggca tatcatctaa attaccagct cctgaaaaca aggccttgct tgcctctagg
      181 gctcggtccg cttcttcttt tccatgtact agttgtgtga gttcataggc cagtatctct
      241 ttagccttgt taatattctt atctggagcc aaaagctcac taattttatc catttctaag
      301 aaggtgtagc gcattagcaa ggtttccacc gaagcgtctt ctacatttct ccagtactgg
      361 aaaaactcat aggggctagt tttttcttta tccaaccaaa gagcgccttt ttgtgttttt
      421 cccattttta ccccagcaga ggtagtgatt agattaaagg taagcccata acaatcgacc
      481 ttttctactc ccctaattaa atcaattcca ccgatgatat tgctccactg atcactgccg
      541 cccatttgta agcggcaacc ctcacttcgg tacagatgaa ggaaatcata ggcttgaaga
      601 ggcatataac taaactcaaa ataagtgagt ccctgctcta gtcttgattg gtaagaatcc
      661 atcttaatca tacgattgat actaaaataa gggccataat ctcttaaaaa ttcaatataa
      721 ttaaggtcta ccagccagtc tgcattattg agcataaggg cttttccctc ttcaaaatca
      781 ataaaccgac ttacctgttc tttaatcttt gctgcattgt ggtcgatatc ttctctggtt
      841 aaaatttgcc gcatatcgtc cctacctgat gggtcaccta tcatggtggt cccgcctcct
      901 aagagtaaaa tgggccgatg gccagcttct tgcattcttt tcataacaac taaggttaaa
      961 aagtgacccg catgcaggct atctgctgtg gcatcaatgc caatataaaa gctgactttt
     1021 tctttttcta aaagatttct caattcttct tcgtgagtag actgagaaat ataaccccgt
     1081 cttactaatt catcatatat tttcatctct cctccttata tatggatagc ttaccaacca
     1141 acccttgctt cgtcaatcta gaaacatgta aggaagcttt ttaattatcc ctttcattca
     1201 agattgagtt tgaagatctc tagccagggt agaaataagt agtaaggtat attaaagaaa
     1261 aggagcatta catgaattgg ttactgctaa accctgtata tttttggttg gttatgggtg
     1321 gtatattttt gatcgttgaa ggggtgacct atgccctggt tacaatttgg tttgtcggag
     1381 gcgctttggt cgcccttctc cttgcctatt taggagttgc ttttggattt caatttgcgg
     1441 catttataat tgtttcattc gcgctgctcc ttataactag accccttgtc aaaagaaaat
     1501 ctgaaaatat cacacacaca aatgtcgatg cggtcctagg acaaactgga attgttacca
     1561 aagaactttc tgaacatttc tccggtcaag gaaaagttgg cggacaaatc tggaccattg
     1621 ctagcctaga tggtcaatgc attgatgtgg gaaaagaatt tgttgtgctc tctgttgagg
     1681 gcgttacact tgttgttgaa cccttagagg aggaagtatg ttccaaataa tccctatcgc
     1741 tataattagt atcatcgttc tgtatatcat cgttagtaat atcagaatta tcccccaatc
     1801 tcaggcctat gttattgaaa ggctgggagc ctttcaacaa acttggggaa caggtctgca
     1861 tataaaaatt cctttttttg aaagaattgc tgctcgagtt tctttaaagg aaagagtagt
     1921 ggactttgag cctcagcctg ttattactaa ggacaatgtc acaatgatga tcgacacagt
     1981 agtctattat cagattacag acccaaaact ctatgcctat ggggtagaaa atccccttag
     2041 tgctattgag aatctaacgg ctactactct aagaaatatt attggtgatt tagagctcga
     2101 tgagtctcta acaagccggg atatcattaa tacccaaatg agaagtatcc ttgatgaagc
     2161 aacagatcca tggggaataa aagttaatcg tgtagaactt aaaaatattc ttcctccaaa
     2221 agatatacaa gatgctatgg aaaagcaaat gcgtgccgag cgtgaaagaa gagaatctat
     2281 tcttcgtgct gaaggacaaa aacgttcagc catcctaatt gctgaaggaa acaaagaatc
     2341 tcagattctt gaagccgaag cctataggaa tgctcgaatc aaaagagcag agggagatgc
     2401 ccaaggtatt gtgcttgtac aaaaagccta tgctgaagga ttagaaattc taatgagtaa
     2461 ggtgccttca gatgtattta tcccactaaa gggatttgaa gctcttgaga agattgcaga
     2521 tacaccatcc tccaagctta ttattccttc agatcttcaa ggtctggcgg gaatggctgc
     2581 tagtacggct gaattcctta aaaaataaag ctaagacaag aaaaatagaa tacaatcttt
     2641 ataaaaaacc cctgcttata actacctagc aggggttctt tttatacaat aaacatcttt
     2701 gttgttaata cttccattat atccttgcct tcaaatacaa tttcattggg gctaaccctt
     2761 tgaactcctg gataaaagga ggctcggtgg gccaagtcat gcagcctaaa ctgaaaacta
     2821 acatgataat attctggctc tttttgaata agttgatctt ttttcttaag tccctctgta
     2881 acaccctcat aaattttctc tagggcatct tcaggatgca tatctagggt ggcctttcct
     2941 tctgcctctt taacggcaag agtctcaatg ccaagaattg cagctcgagc ctcattgcaa
     3001 acttcttggt ctccacttat aaaaacacaa ggaacgccat aataattagc aagttgtcgg
     3061 ttaaaactaa actcagaagc aattttccca tttatactac aagtaaaaat ctttgagtag
     3121 ctaaaactgt gggctagagg attgccatct tttcctccag atgtatggta gccaataaaa
     3181 acaacagcat caaaactttc atcaagccca gccatcattg cctttggaca agctgtccaa
     3241 ccacgaataa ctcttactcc tctgggaagc tcattaataa gtatattccg ggcagaatcg
     3301 tgggcgtctt taacaattac ttcatagccc aaatctatgg cagcctgcgc agcagcagct
     3361 acttctctag tcatctgtct ggccgctttt atatgatcct tatggttaag atctgtttca
     3421 tcccaagctg ttacaccggt aattccttcc atatctgcag atataaatac tttcactttt
     3481 aactcctttt ggttagttca accatgattt aaataaaggc ctgtctttgt atgacccaaa
     3541 gtctcccttg tttttcccct taaatactcc ttagatttgc aaagttccct ctgaatacat
     3601 tttctgattt ttaaatagag tgctttggaa aaaagataga accatcttac acttattttt
     3661 aagcttattt gatttttaaa atcaaataag ctatgttact gctctttcat ctactctacc
     3721 atagtaagat aaaatcaaat aatcctgtcc aaattggctc taagataac
//
LOCUS       DASRNA010000133         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_632509,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000133 DASRNA010000000
VERSION     DASRNA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632509"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1160
                     /locus_tag="VFD08_03655"
     CDS             141..1160
                     /locus_tag="VFD08_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HZK10147.1"
                     /translation="MDKVLVLGAGVMGTALAVYLAENNRAVNLWGTKWDKEILTQMEQ
                     TRKNKALEKDIPKNVNLFYEEELEEAFEDISLIVIAVSTKGIESVSKALGPYLKENHM
                     ILSVTKGIDEEHLYTMSDVIKKALPERLRDKVDIIKLGGPIIAKEIANGKYTEGIFAS
                     KNIEAARYARYIFHSSKFKGNTTTDIEGVELCAAFKNPYAIAVGIVQGMEGGMNNSKA
                     ALMARGTIEMANILEAYGSSRETALGLAGIGDYYVTSQGGRNGIFGSLLGKGKTVEEA
                     LEIMDYQTVEGLATTLSGYKLLKKLEKEKNFDIKDKAFLFNELYDVLYKGKDVKKAMS
                     NYWGS"
     gene            1205..1399
                     /locus_tag="VFD08_03660"
     CDS             1205..1399
                     /locus_tag="VFD08_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017472048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HZK10148.1"
                     /translation="MDGKSRDNIKVGKRVKIVQKQDQRSGNLTEGLVSKILTKSKNHP
                     HGIKVMLENGLVGRVKEILD"
     gene            1758..>2455
                     /locus_tag="VFD08_03665"
     CDS             1758..>2455
                     /locus_tag="VFD08_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase"
                     /protein_id="HZK10149.1"
                     /translation="MKTFTKFQLRDLTLKNRIVMPPMCMYSSDESGEVKHFHKIHYGS
                     RAIGGTGLIILEATAVIANGRISNRDLGIWDDKQVEGLKSLVDLIHENGAKAAIQLAH
                     AGRKSDSGNDYIVAPSPISHSDKYDHPRELGQADIADLVERFKKGAKRSLEAGFDALE
                     IHAAHGYLIHQFLSPITNKRVDEYGGSLENRTRFLKEILMAIEEVWPKEKPIIVRFSA
                     TDYTEGGIDLDEIVA"
BASE COUNT          928 a          359 c          569 g          599 t
ORIGIN      
        1 gcacatatat gattggtata ataaactccg ctagtagatg actagtaggg gagtataaga
       61 aattatggaa gtagtatctt gcaagactta taagataata aggaaaaaga cagtctttta
      121 cagaattggg aggattgata atggataaag tattggtttt aggtgctggg gtaatgggaa
      181 ctgcactagc agtatattta gctgagaata atcgggctgt aaatttgtgg ggaacaaaat
      241 gggacaaaga aatactcaca caaatggagc agacaagaaa gaataaagcc cttgaaaaag
      301 acataccaaa aaacgtaaac cttttttatg aagaggagct agaagaagcc tttgaagata
      361 taagtttgat tgttatagca gtatctacta aaggaataga aagtgttagt aaggccctgg
      421 gcccctatct aaaggaaaac catatgatcc ttagtgtaac caagggcata gatgaagaac
      481 acttgtatac catgtcggat gtgattaaaa aggcactacc agaaagacta agagacaagg
      541 tagacattat caaactgggt gggccaatta ttgccaaaga aatagccaat ggaaaatata
      601 cagagggtat atttgcttca aaaaacatag aggcagctag gtatgccaga tatatatttc
      661 actcttcaaa attcaaagga aatacaacaa cagatataga aggagttgag ctttgtgcag
      721 cctttaaaaa tccctatgcc atagcggtgg gaatagtcca ggggatggag ggaggcatga
      781 ataattcaaa ggcagccctt atggcaagag gaacaataga aatggccaac atcttggaag
      841 cctatggaag tagtagagaa acggccctag gcctagcagg gattggggac tattatgtaa
      901 cctcccaggg gggaagaaat ggaatttttg gcagcctctt aggcaaggga aaaactgttg
      961 aggaggccct agaaattatg gattatcaaa cagtagaagg tctggcaaca accctaagtg
     1021 gatataaatt actgaaaaaa ctagaaaaag agaaaaactt tgatataaag gacaaggcat
     1081 ttttatttaa cgaattatat gatgtcttat ataagggaaa agatgtaaaa aaagccatgt
     1141 caaactactg gggtagctaa tcctagagaa aacaaagtag acaaaaataa aagggagtag
     1201 aatgatggac ggaaaatcgc gagataatat aaaggtaggc aagagggtta agattgtaca
     1261 aaagcaagat cagcgctcgg gcaatctaac agaaggtctt gtttctaaaa tacttacaaa
     1321 atcaaagaac catccccatg gtataaaagt tatgttagaa aatggtcttg tgggaagagt
     1381 aaaagaaatc ctagactagt acttatccaa gacaatgctg aaatatagat tagataaacc
     1441 ttattactca attagttggg tatagataaa actaaggaaa acagattcaa caaaagcagc
     1501 agttttaaac taagaaaatt ccaatagaat aactcgattg gatgagaaat tatgattctt
     1561 atatagacca agtaagtaag gcgggcaaat caaaagagaa atggacatgg gtgaagctgc
     1621 ccttattgca gcctctttag aagctcgttg aatcggctgc gaaagatatg ttagatggcg
     1681 cagggataag aaaagttgat aaatgtacaa atttacaggt agaacaataa ataagataag
     1741 tgaataagga gatttatatg aaaactttta caaaatttca attgagggat ttaaccctta
     1801 aaaacagaat cgttatgcct ccaatgtgca tgtattcttc cgatgaaagt ggtgaggtca
     1861 agcatttcca taagatccat tatggatcaa gagccatagg agggactggt cttatcattt
     1921 tggaagcaac ggctgttatt gcaaatggca gaatctcaaa tagagattta gggatttggg
     1981 atgataagca ggtggaaggc ctaaagtcac tggtagatct tatacatgaa aatggggcta
     2041 aagcagccat acaattagcc catgctggta gaaaaagtga tagtggaaat gattatatcg
     2101 ttgccccctc acccataagc catagtgata aatacgacca tccgagggag cttggacaag
     2161 cagatattgc agatttagtg gaacgcttta aaaaaggcgc taaaagatcc cttgaagcag
     2221 gctttgatgc gctagagatt catgcagccc atggatattt aatccatcaa ttcctgtctc
     2281 ctattaccaa taaaagagtc gatgagtatg ggggcagtct ggaaaataga acgagatttt
     2341 taaaagaaat attaatggcc atagaggaag tttggccaaa ggaaaaacca ataattgtga
     2401 ggttttctgc aacagactat acagaaggcg gcatagatct tgacgagatt gttgc
//
LOCUS       DASRNA010000134         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_937422,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000134 DASRNA010000000
VERSION     DASRNA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937422"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VFD08_03670"
     CDS             <1..994
                     /locus_tag="VFD08_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZK10150.1"
                     /translation="ILIILLMLVLLVSCKPSQPLVRQDFALATVVKISLRDHASEELM
                     DKIFARLWEIEEGMSIDGKDSDIYKLNQAKGQAIQVKDDTYYVMKKAKQYYTLSQGYF
                     DPSLGNVVDLWKIGTEEARVPSDKEIKEALAYADGSQIELLENYHVRIPQGTILDLGA
                     IAKGYAADEAARIAQEAGVSSAIFDLGGNVLALGQKEGDNFRIGIQEPFSEIVRGSPF
                     AILSLSNQTVVTSGDYERYFEEDGKRYHHILDFRTGYPVENELASVSIITDTSIRADS
                     LSTAVYAMGLEKGKNFVEKQKNLEAIFVTKNKDVIVTEGLLDVFTLEDSSYHLE"
     gene            1008..2846
                     /locus_tag="VFD08_03675"
     CDS             1008..2846
                     /locus_tag="VFD08_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006207772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK10151.1"
                     /translation="MKKIIILGAGYGGILTAKRLEKTFRKREDVEITIIDKNTYHAML
                     TELHEVAAERVPREAVRIDLDKIFAKRNIEVVHDEVKDIDFDQKRLVGVVGEYNYDYL
                     VLATGSKPTFFNTPGADEHALTLWTYADSLRINNHIHKMLQLATSELDPDKRKAMLSF
                     VVIGSGFTGVEMVGELAEWKSRLCLTYNIEPDDLNIHVIDMQSRVLPIFDEVLSDKAE
                     AYLRKMDVQIHLSTAITAVNEDSIEVKGRDPIPTRTAIWAAGIEGSELMEGLSLEKVA
                     RNRVKTDKYLRSVDHRDVFVVGDNIFYIPEGQEHPVPQMVENAEISHEVVSHNITSTL
                     DNKEMIEFKPEFHGAMVCIGGRYGVAELNTKNRKFHLSGFPAMFVKHFINILYFMKIA
                     GINKVWTYLLHEFFHVDDRRSFVGGFFSKRSPNFLLLPLRVFLGIKWLLAGLDKFGGV
                     WEDPSKIFLLPSKVVDATAAATEVVEGVEAVSPLPVPEFIWKSGDWFMNLLFYNADGS
                     FNGLAPVFQTFMVVGEILVGLALIAGLFTLLAGLGSLAMSLMIYVSANAAPEILWYIV
                     ATMAVMVGSGSTFGLDYWVLPWLSDRWKKLAFVRRWYLYTDRARGY"
     gene            2848..>3652
                     /locus_tag="VFD08_03680"
     CDS             2848..>3652
                     /locus_tag="VFD08_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZK10152.1"
                     /translation="MKVFERHEGMLQEVDKKLYEFLKTGNSYINGSVDYLSTMKGKML
                     RPLLYLLGTKIAGEKANLEVATALEMLHVATLIHDDVIDESKLRRSQESIQSKYSKDY
                     ALYMGDYLFSSLFLLLSQSPMEREDMLEIAKMMQKICVAEMHQYHQRYNLEMGFLGYL
                     RIVSGKTAALFSLSLAAGSKDEEDRKTLLRLGYRLGVAFQIQDDLLDFQGDEKSVGKD
                     LRQDLLRGNYSLPIILAYESMPEETKNLMEKDPIPEEELFDLLDQSGSLI"
BASE COUNT         1101 a          677 c          833 g         1041 t
ORIGIN      
        1 tattctaata attttactca tgctagttct tctagtgtcc tgcaagccat ctcaacccct
       61 tgtaagacaa gattttgctc ttgctaccgt ggtgaaaatc agcctgcgtg atcatgctag
      121 cgaagaactt atggacaaaa tttttgcccg actatgggag attgaggagg gcatgagtat
      181 tgatggtaaa gactcagata tttataaact caaccaggca aagggacagg ctattcaagt
      241 aaaagatgac acatactatg taatgaaaaa agccaaacag tactacaccc taagccaggg
      301 atattttgat cctagcctgg gaaatgtggt tgacttatgg aaaatcggca cagaagaggc
      361 aagggtccct tctgataagg aaattaaaga agcccttgcc tatgcagatg gaagtcagat
      421 cgaactacta gaaaactacc atgtgcgtat accccagggg accatattag atctaggagc
      481 cattgccaag ggctacgcgg ctgatgaagc agcaagaata gctcaggaag caggagttag
      541 cagtgcgatt tttgatctag ggggcaatgt ccttgcccta gggcaaaagg agggagataa
      601 ttttcgcatt ggtattcaag agcccttttc tgagatcgta agggggagtc cttttgccat
      661 actttcttta tccaaccaga cggttgtaac cagcggagat tatgagcggt attttgaaga
      721 agacggaaag cgttatcatc atattttgga ctttcgcaca ggctatcctg tggaaaatga
      781 actcgcctca gtatctatta ttactgacac ttctatacgc gctgattccc ttagcactgc
      841 agtctatgct atggggttgg aaaaaggaaa aaactttgta gaaaaacaga agaatctaga
      901 agcgatcttt gtaacaaaaa ataaagatgt aattgtaacc gagggattgc ttgatgtttt
      961 tacactggaa gactcaagtt atcacctaga ataatcgagg agaatgaatg aaaaagatta
     1021 ttatcctggg ggctggttat ggtggaatat taactgccaa aagactggaa aagaccttcc
     1081 gtaaaagaga agatgttgaa ataactatta ttgataaaaa cacctatcac gctatgttga
     1141 ctgaactcca cgaggttgct gccgagcgtg ttccccgtga agcagttcga atcgacctag
     1201 ataagatttt tgcaaaaaga aatattgaag ttgttcacga tgaagtcaaa gatattgact
     1261 ttgatcaaaa aagactagtg ggtgtagtag gggaatataa ctatgactat ctggttcttg
     1321 ctacaggatc aaagcctacc ttttttaaca cccctggtgc agatgaacat gcactaaccc
     1381 tttggaccta tgcagattca cttcgaatca ataatcatat ccacaagatg cttcaacttg
     1441 ccacatcaga attagaccct gacaaaagaa aagccatgct tagttttgtc gttattggta
     1501 gtggatttac tggtgttgag atggtaggag agcttgccga gtggaagagc cgtctttgcc
     1561 taacctataa tattgaaccc gatgatctta atattcatgt tatcgatatg cagtctagag
     1621 tcttacctat tttcgatgag gtgctttcag ataaggcaga agcctatcta aggaagatgg
     1681 atgtgcaaat tcatctaagc actgctatta ccgcagtgaa tgaggactct atagaagtta
     1741 agggaagaga ccctatcccg acaagaactg ctatttgggc agctggcatt gaaggcagtg
     1801 aactcatgga agggcttagt ctagaaaaag tcgcaagaaa ccgcgttaaa acggacaaat
     1861 accttcgttc agtagaccat agagacgttt ttgttgtagg agataatatt ttctatattc
     1921 ctgaaggcca ggagcatcct gttccacaaa tggtggaaaa tgcagaaatc tcccacgagg
     1981 tggtcagtca taatattacc agtactttag ataacaaaga aatgatagag tttaagccag
     2041 aattccacgg cgccatggtc tgtatcggtg gtcgctatgg ggtagcagaa ctaaacacga
     2101 aaaacagaaa gtttcacctt tcaggttttc ctgccatgtt tgttaagcat tttatcaata
     2161 tcctatactt tatgaaaatt gctggaatca ataaagtttg gacctatcta ttacatgagt
     2221 ttttccatgt agacgataga agaagttttg tgggtgggtt tttctcgaaa cgttccccta
     2281 actttttgct tcttcccctt cgcgtttttc tagggatcaa atggctatta gcaggcctag
     2341 ataagtttgg aggtgtttgg gaggacccaa gcaagatttt tcttctgcca agtaaagttg
     2401 tcgatgctac agcagcggca accgaagtgg tagaaggagt agaagcggtg agtcctcttc
     2461 ccgttccaga atttatttgg aagagcggag attggtttat gaatttgctg ttttataatg
     2521 ccgatggcag ttttaatgga ctagcccctg tctttcaaac ttttatggtg gtgggagaaa
     2581 tccttgttgg gctggccctt atagctggac tctttacctt gctggctgga cttggctccc
     2641 ttgctatgag tcttatgatc tatgtcagcg caaatgcagc ccctgaaatc ctgtggtata
     2701 ttgtagctac catggcagtt atggtaggtt ctggaagcac ctttggtctt gactactggg
     2761 ttcttccctg gctatctgat cgctggaaaa agctagcctt tgtgcgtagg tggtatctct
     2821 atacagatag agcccgaggc tattaagatg aaagttttcg agcgccatga gggaatgctc
     2881 caagaggttg ataaaaagct ctatgagttt ttaaaaacag gaaatagtta tatcaatgga
     2941 tccgtagatt atctctctac tatgaaaggc aaaatgcttc gtccccttct ttatttgttg
     3001 gggacgaaga ttgctggaga aaaggccaac ttagaagtag caacagcctt agaaatgctc
     3061 catgtagcca cactaatcca cgatgatgtt attgatgagt cgaaacttcg gcgcagtcaa
     3121 gaaagtatcc agagtaaata ctccaaggat tatgcccttt atatggggga ttatcttttt
     3181 agctctcttt ttcttcttct ttcccagtcc cccatggaaa gagaagatat gctagaaata
     3241 gcaaaaatga tgcaaaaaat atgcgtagca gagatgcatc aataccatca gcgttataat
     3301 ttagaaatgg gttttttagg ctatcttcgg attgtatctg gaaaaacagc agctcttttt
     3361 tcattaagcc tagctgcagg tagtaaggat gaagaagaca gaaaaaccct tcttcgccta
     3421 ggctatcgct taggtgttgc ctttcaaatt caagatgatc tcctagattt tcaaggagat
     3481 gaaaagtcag tgggaaaaga tttgcgtcag gatttactta ggggaaatta cagtctgcct
     3541 attattctag cctatgaatc catgccagaa gaaaccaaaa acttgatgga aaaagaccca
     3601 attcctgaag aagaattgtt tgatttgcta gatcaaagtg gatctttaat aa
//
LOCUS       DASRNA010000135         5886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_481599,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000135 DASRNA010000000
VERSION     DASRNA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481599"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1244)
                     /gene="xseA"
                     /locus_tag="VFD08_03685"
     CDS             complement(84..1244)
                     /gene="xseA"
                     /locus_tag="VFD08_03685"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZK10153.1"
                     /translation="MNRLSVSQLNMLISRELALSPRLNKVEVEAEIGRLTRHGSGHYY
                     FTIKDHYASIDCVMYRSSVEKNSYNFEEGDRVLLKGNVSFYEKSGRLQFYVQSVKALG
                     EGELLKAFYALKEKFLAQGLLDPLNKKPLPPYPENIAILTSPTGAAYKDFMKIFALRN
                     PFCKVTLYPVAVQGDKAVESIIEALKIAQADLIVLTRGGGSYEDLSVFNDERLCLQIA
                     EHSTPCLCAIGHEIDFTLAELVSDQRAATPSQAAQLATEDMIGLLDSLKSKLDLHAKN
                     RFVELEKQSFYIESKIDKMRRYFEQRLINEHRTLFDYQKNLQDSFQGIFEKEKEKTKL
                     SFLKIESYNPDKILEKGYIAIKQDHWIVRKDNLLPGPAELYFYDGIKKVEIK"
     gene            complement(1237..1632)
                     /gene="nusB"
                     /locus_tag="VFD08_03690"
     CDS             complement(1237..1632)
                     /gene="nusB"
                     /locus_tag="VFD08_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZK10154.1"
                     /translation="MTRRTMRRKLMQWIYSCEIQNNLENLFKEFDFEEIKEERELHYA
                     HDLLENLAENVVFIDKLLDQLSHTWPVNRMNKVDLALLRLATTEILYLADIPHEVSIN
                     EAMEISKIYSSPDSYSFINGVLGSVVDYE"
     gene            complement(1632..1973)
                     /locus_tag="VFD08_03695"
     CDS             complement(1632..1973)
                     /locus_tag="VFD08_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZK10155.1"
                     /translation="MEKVLTTKLGNLSISQEAITELITIAAKEIEGVVLVQQDTPSQI
                     INFLKRKPLEAIQFEEGSEGLILNLNVTLENGIKITDTALKIQENLKSALETMLEIPV
                     SRVDIHIVGLK"
     gene            complement(2053..2391)
                     /locus_tag="VFD08_03700"
     CDS             complement(2053..2391)
                     /locus_tag="VFD08_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10156.1"
                     /translation="MSYIREKLNFLQGYSEGLELDESKKETKLILGILDLLDEMSYML
                     DELDEEVAVLDEVVEAIDEDLTDLEVDFYDMDDELYFEEYDDEDDDEDLFSPDYDAES
                     FELYDEEDEE"
     gene            complement(2405..2962)
                     /gene="efp"
                     /locus_tag="VFD08_03705"
     CDS             complement(2405..2962)
                     /gene="efp"
                     /locus_tag="VFD08_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZK10157.1"
                     /translation="MISANDFRKGITFEIDGQPYVVIDFQHVKPGKGAAFVRTTYRNI
                     ISGATREETFNPSDRFNRARIERKNMQYLYKDGTNYVFMDNESYDQLELTQEQVADVI
                     KYVRENDSVDVRFFLGKVIDVEAPNFVELEVVESEPGIRGDSASNITKKATLETGADV
                     NVPLFVNVGDTIKIDTRTGEYLSRK"
     gene            complement(2959..4026)
                     /locus_tag="VFD08_03710"
     CDS             complement(2959..4026)
                     /locus_tag="VFD08_03710"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HZK10158.1"
                     /translation="MLNQIRKSCKELSLDACLFYKPENRYYVSGFTGSTGYALILEDK
                     TYFITDFRYISQVEEECPDFEIIEISASKSLGDVLNELKLNSLGFEDDFMSVESLESL
                     KGNFHGELVPMRRFIEKIRQIKSNDEIALIKKAQSIADDTFTYLLDFIKAGMTERDVY
                     LELVNQLKKRGAEGESFKAIVASGVRGALPHGSATDKVIKEGELLTLDFGCIYKGYCS
                     DMTRTIAIGKANKKQKEIYEIVLHAQKEALKAIKPGAICKEIDKIARDIITEAGYGEN
                     FGHGLGHAVGLEVHENPRLNPTDDSLLEAGMVVTDEPGIYIPGFGGVRIEDLVLVTDT
                     GAQTLSTSNKELIELEVQEAL"
     gene            complement(4131..5351)
                     /locus_tag="VFD08_03715"
     CDS             complement(4131..5351)
                     /locus_tag="VFD08_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HZK10159.1"
                     /translation="MKKIELLAPAGDLEKLRYALHYGADAVYMAGEVFGLRQQTKNFT
                     IEEMEIALDEAHSMGKKLYLVLNIIPHNEDIKELKTYLEQIKNLCFDAYIVSDPGAIV
                     LVREILGDVEIHLSTQANTTNALSARFWHSQGVKRIILARELSLIEIKEIVDATQDLD
                     LEYEVFIHGAMCISYSGRCLISNFLSGRDANKGDCAQSCRWSYYLMEEKRPGEYLPVV
                     EDEKGTYLYNSKDLCAIDLLKEIIDTGVVSLKIEGRNKSIYYVSSIVRAYKEGINRIQ
                     KNRALNSEELFKEISRVSHRDFTQGFFLRKPTEEDQLYNAQNYIRRADFLGVVLDYDE
                     NTKLVKIEQRNKFELGDYVDFIGPDYKSYRLELNELFDHDMKPIKAAPHPQQIIYIYS
                     KQKLYPMDLMRKAF"
     gene            complement(5348..>5886)
                     /locus_tag="VFD08_03720"
     CDS             complement(5348..>5886)
                     /locus_tag="VFD08_03720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK10160.1"
                     /translation="LEADRVGVSVISNEVGEFLAFYLKNKIVKELVEIGSGYGYSTKL
                     LHLLLPKANIVSLERHIPRYEVAKDFLSHPNIEFVLGDGQDYLAKRKKKIDLLFLDGS
                     KPHYVYFLQEALAHMNKGSLLIADNIFARGLSYDSKCDRRHRTLQRRMHAFLELVFEE
                     FHATILPIDDGLLIGEKK"
BASE COUNT         1710 a         1209 c          994 g         1973 t
ORIGIN      
        1 atcaagagct ttatctaagg ccatagtatc agaaagttta ctttctattt cttctaaacg
       61 caggatcttt ttttgatagt ctttcatttt atctctactt tctttattcc atcgtaaaaa
      121 tacagctcag ccggtcctgg aagcaagttg tcttttctta ctatccaatg atcttgcttt
      181 attgcaatat agcctttttc taatatttta tctgggttat aactttctat tttgagaaaa
      241 gatagttttg ttttttcttt ttccttttca aaaatacctt gaaaactatc ttgcagattt
      301 ttttggtagt caaagagtgt acgatgctca ttgataagcc tttgctcaaa atagcgacgc
      361 attttatcaa ttttactttc tatataaaaa ctttgttttt cgagttcaac aaagcggttt
      421 tttgcgtgaa gatcaagttt gcttttaagt gaatccaata gaccaatcat atcttctgtc
      481 gcaagttggg ctgcctggct tggggttgct gccctttgat cacttacaag ctctgctaag
      541 gtaaagtcta tttcatgccc aatggcacaa agacagggag tactatgctc tgctatttga
      601 agacagagtc tctcgtcatt aaagacagat agatcctcat agctgccacc cccacgggtg
      661 agaactatca gatctgcttg tgctatttta agggcttcta ttatcgattc tactgcctta
      721 tccccttgaa cggcaaccgg ataaagagtt actttacaaa aaggatttct tagggcaaat
      781 atcttcatga aatccttata ggctgctcct gtgggagaag ttaaaattgc aatattttca
      841 ggataagggg gcagtggttt tttatttaag gggtctaaaa gtccctgtgc aaggaacttt
      901 tcctttaagg cataaaaagc ctttaggagt tctccttctc ctagagcttt aacgctttga
      961 acataaaact gcagtcttcc agatttttca taaaaagaaa catttccctt taaaaggacc
     1021 cggtctcctt cttcaaaatt ataactattt ttttcaacac tagagcgata cataacgcaa
     1081 tcaatagaag cataatggtc tttaatagta aagtagtaat ggccactacc atgacgggta
     1141 agccgtccaa tttcagcttc aacttctact ttgttcaagc gtggagacaa agctagctct
     1201 cgtgagatca acatatttag ttgagaaaca cttaatctat tcataatcaa ctacactgcc
     1261 taatacgcca tttataaaag aataggaatc tggactggaa taaattttag aaatttccat
     1321 agcttcattg attgatactt catggggaat atctgcaaga tagagtattt ccgtagttgc
     1381 tagccgaagc agtgctaaat caactttatt cattcgattg acaggccatg tatgactcag
     1441 ttggtctaat agcttatcga taaatacgac attctctgct aggttttcta acaggtcgtg
     1501 ggcatagtgc agttctcttt cttctttaat ttcttcaaaa tcaaattctt taaatagatt
     1561 ctctagattg ttttgaattt cacaggaata aatccactgc atcagttttc gccgcattgt
     1621 tcgtcgggtc attatttaag tcctacaata tgaatatcaa cccttgaaac agggatctcg
     1681 agcattgttt caagcgcaga ctttaaattt tcctgtatct taagtgctgt atcagttatt
     1741 ttaatgccat tttcaagagt aacatttaaa tttagtataa gcccttcact tccctcttca
     1801 aactgaattg cttctaaggg ctttcttttt aaaaagttta taatttgtga aggtgtatct
     1861 tgttggacta aaactacgcc ctcaatctct ttggcggcaa ttgtaataag ttcagtgatg
     1921 gcctcctggc ttatactcag attgcctaat tttgttgtta atactttttc cataatatac
     1981 ctccgctact atgaattata ccaaaaaaaa cgaacctctt ccacttgaag cagaaaaggt
     2041 ccgaatttgc tattattctt cgtcttcttc gtcgtaaagt tcgaaacttt cggcgtcgta
     2101 atctggagaa aacaaatctt cgtcatcgtc ttcgtcatcg tattcttcga aatacagctc
     2161 atcatccata tcataaaaat ctacttcaag atcagttaaa tcttcatcaa tggcttctac
     2221 gacttcatcc aagacagcaa cttcctcgtc tagctcatcc agcatataac tcatttcatc
     2281 gagaagatcc agtatcccga ggattagctt cgtttccttt ttgctttcat caagctccaa
     2341 accctcagaa tagccctgca gaaaatttaa tttttcacgg atatagctca ttttttcctc
     2401 ctgattactt cctggacaag tactctcctg tcctagtgtc gattttgatt gtgtcgccga
     2461 cattgacaaa tagtggaaca ttgacatcag cgccggtttc aagggtcgcc ttctttgtta
     2521 tattggaagc gctatctcct cgaattccag gctcagattc aacgacttca agctctacaa
     2581 aattcggagc ttctacatcg ataaccttgc ctaagaaaaa gcgcacatcc acagaatcat
     2641 tttcacgaac atacttaatt acatcagcaa cctgttcctg ggtaagctct agttgatcat
     2701 agctttcatt gtccataaag acataatttg ttccgtcttt ataaagatac tgcatatttt
     2761 ttcgttcaat gcgagctcta ttaaagcggt ctgatggatt aaatgtttct tccctagtgg
     2821 ctcctgaaat aatgtttcga taagtggtac gtacaaaggc tgcaccttta ccaggcttaa
     2881 catgttggaa atcaattaca acatagggtt gaccatcaat ttcaaaggtt attccttttc
     2941 taaaatcatt ggctgatatc ataaggcctc ctgaacttct aactctataa gttctttgtt
     3001 tgatgttgaa agtgtttgag cacctgtgtc ggtaactaaa acaagatctt ctatacgtac
     3061 gcctccaaag cctgggatat aaataccagg ttcatcggtg acaaccatac cagcttctaa
     3121 aagcgaatca tctgtgggat taagtcgagg attttcatgg acttcaagac ctactgcgtg
     3181 acctaggcca tggccaaaat tctcaccata gccagcttct gtaatgatat ctctagcaat
     3241 tttatcaatt tctttacaga tagcgcctgg cttgatagct tttaaggcct ctttttgagc
     3301 atgaagcaca atttcataaa tttctttttg ttttttattt gctttgccaa tagctatggt
     3361 tcgtgtcata tccgaacagt agcccttata aatgcatcca aaatccaggg taagaagttc
     3421 gccttcttta attaccttat ctgtagctga gccgtgaggc aaggctcccc tcacccctga
     3481 ggcgacgatg gctttgaagg actcgccttc agcaccacgt ttttttaatt gatttactag
     3541 ttcaagataa acatctcttt ctgtcatacc agcttttata aagtctagca gataagtaaa
     3601 ggtatcatct gcaatggatt gagctttttt aattaaagca atctcatcat tgcttttgat
     3661 ttgccggatc ttttcgatga atcgtctcat gggaacaagt tctccatgaa agtttccttt
     3721 gaggctttct aggctttcga cgctcataaa atcatcttca aaaccaaggc tatttaattt
     3781 cagttcattc agtacatctc ccagtgattt actagcgctt atctcaataa tttcaaaatc
     3841 tggacattct tcttccacct gagaaatata gcgaaaatca gtgataaaat aggtcttatc
     3901 ttctaggata agggcatagc cagtactgcc agtaaaacca ctgacataat aacgattttc
     3961 tggtttataa aataagcaag catctagcga taattcctta caacttttac ggatttgatt
     4021 taacaccttc aaaagatcct ccttatcata ttctaggttc attctatcat agctttggga
     4081 caaaactcaa caaaacttta aaagtttttc cccatcaact gtaaagtatt ttagaaagct
     4141 ttacgcatta gatccatggg ataaagtttt tgcttactgt aaatataaat aatttgttga
     4201 ggatgaggtg cagctttaat gggcttcata tcatgatcaa agagctcatt aagttctagc
     4261 ctataacttt tataatcagg accaataaag tccacataat cgcctagttc aaatttatta
     4321 cgttgttcga ttttcacaag ttttgtgttt tcatcgtagt ctaaaactac cccaagaaaa
     4381 tcagctcttc taatatagtt ttgggcattg tatagttggt cttcctctgt gggttttctt
     4441 aaaaagaaac cttgggtaaa gtctctatgg gagaccctgg aaatttcttt aaagagttct
     4501 tcgctattaa gggctctatt tttttgaatt cgattgattc cttctttata ggcacgaaca
     4561 atagaagaaa cataatatat ggacttgttc cgcccttcta ttttaaggct aacgactcct
     4621 gtatcaataa tttcttttag aagatcgata gcacaaaggt ccttactgtt ataaaggtag
     4681 gtccccttct catcttcaac aactggcaga tattcgccag ggcgtttttc ttccattagg
     4741 tagtaactcc agcgacatga ttgtgcacag tctcctttat ttgcatcgcg acctgaaaga
     4801 aagttgctaa ttaaacagcg accactatag gagatacaca tggcaccatg aataaaaact
     4861 tcatattcaa gatctaaatc ctgggttgca tctacaattt cttttatttc aattaaactg
     4921 agttccctag caaggataat gcgttttacc ccttgactat gccaaaagcg agcggataaa
     4981 gcattggtgg tattagcctg ggtacttaaa tgaatctcca catctccaag aatttctcta
     5041 acaagaacaa tagcgcctgg atcagatacg atatatgcat caaagcaaag atttttaatt
     5101 tgttccaaat aagttttaag ttctttaatg tcttcattat gggggattat attaaggaca
     5161 aggtaaagtt ttttccccat ggaatgagct tcatccaggg caatttccat ttcctctatg
     5221 gtaaaattct tagtttgttg tcgaagtcca aagacctcac ctgccatata gacggcatca
     5281 gctccataat gtagagcata acgaagtttt tccaaatccc ctgccggtgc taaaagttct
     5341 atttttttca ttttttttct ccaattaaaa gaccatcatc tattggaagg attgttgcat
     5401 gaaactcctc aaagacaagt tctaaaaagg catgcatgcg ccgttggagg gttctatgcc
     5461 tacgatcgca cttgctatca tagcttagtc ctctagcaaa gatattatca gctattagca
     5521 ggctgccctt gttcatatga gcaagagctt cctgtaagaa atacacatag tgaggtttgg
     5581 atccatcaag aaaaagaaga tcaatcttct tttttctttt agcaagataa tcctggccat
     5641 caccaaggac aaattctata ttaggatggg ataaaaaatc ttttgccact tcatagcgcg
     5701 ggatatgccg ctcaaggctt acaatatttg ccttaggcaa aagaaggtgg agaagtttag
     5761 tagaatatcc atatccactt ccaatctcca ctagttcttt aacgattttg ttttttagat
     5821 aaaaagcgag gaattcacca acttcattgc taataacact cactcctacc ctgtctgcct
     5881 ctaaac
//
LOCUS       DASRNA010000136         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_708529,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000136 DASRNA010000000
VERSION     DASRNA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708529"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..945
                     /gene="meaB"
                     /locus_tag="VFD08_03725"
     CDS             16..945
                     /gene="meaB"
                     /locus_tag="VFD08_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZK10161.1"
                     /translation="MNYKEVGWENKRQIARMISMVENAAPGHEEIFEKIYDKTGHAYV
                     IGITGPPGAGKSSLTDLLARNYISQEKKVGIIAVDPTSPFSGGSILGDRIRMNELTTD
                     PRVFIRSMGTRGQLGGLSAGTASALQVLDAVGADIIIIETVGVGQSEVEIIKYADSIL
                     MVMVPGLGDDIQTMKAGIMEIGDVFVVNKSDLPGANKTKLELEAMLSFMQNKKWRPPV
                     LLASASRNEGIKELVEALDNHRTYQEKEGLLFERRKRNINELLFEVLQDKFFRQIKEL
                     EDYPQLLKDLTRKEIDPYTAAQLLLKQLKEKRG"
     gene            946..1353
                     /gene="mce"
                     /locus_tag="VFD08_03730"
     CDS             946..1353
                     /gene="mce"
                     /locus_tag="VFD08_03730"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZK10162.1"
                     /translation="MEILKLDHIGIAVKSLDDALPFWRDVLGLKEAGIEVVEEQKVKV
                     AFLPVGDTEVELLESTDEDGPIARFIEKNGPGVQHLAFRVKDINAGIDYMLEQGMRMI
                     DSSARYGAGGARIAFVHPKSSQGVLVELSERED"
     gene            1372..2910
                     /locus_tag="VFD08_03735"
     CDS             1372..2910
                     /locus_tag="VFD08_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZK10163.1"
                     /translation="MDKNKLLIDKKETIIAGGGSKRIEKQHASGKLTARERLALLFDK
                     DTFIELDAFVSHRCTNFGMEKISAPGEGVITGYGYVDDRLVYAYAQDFTVIGGSLGEM
                     HAEKIIKAHENAMKVGAPFVGLNDSGGARIQEGVDALSGYAKIFYANTMASGVIPQIT
                     AIMGPCAGGAVYSPALTDFVFMVDQSAKMFITGPQVIETVTGEKVSAEELGGSMTHNS
                     VSGVAHFHNKSDEDTLAQIRRLISFLPSNNAQRAPSRDPGEVQGYLEDLDHIIPESSS
                     KPYDMHKIIESLVDHGDFMEYQAYFAKNIITGFARIGGRSVGIVANQPQVLAGTLDVN
                     ASDKAARFIRTCDAFNIPLLTLVDVPGFLPGTDQEYAGIIRHGAKMLYSYSEATVAKV
                     TLIVRKAYGGSYIAMCNKELGADVVYAWPTAEIAVMGPKGAANIIFRKEISAAEDPVA
                     ERQARIDQYDKEFATPYKAAERGYVDDVILPHSSRARIVDAFVMLEGKRASLPRKKHG
                     NMPL"
     gene            2919..3305
                     /locus_tag="VFD08_03740"
     CDS             2919..3305
                     /locus_tag="VFD08_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HZK10164.1"
                     /translation="MKEFASIQDMSQMPIMERLSNGLIVTVLGMGMTILVLVLLYFAI
                     KLMSNILSKEESPKATQSEPIPLAPLKEEKDKEDEELIAVFMAAIMAHTGQDHKKIKF
                     QGYREVGTDLPLWSKAGVMETMNREI"
     gene            3315..3716
                     /locus_tag="VFD08_03745"
     CDS             3315..3716
                     /locus_tag="VFD08_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009656385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZK10165.1"
                     /translation="MKTYKITVNGTVYEVDVEEIREDASSSAPSYLAPSSAPRPATSP
                     APAPTPTPVAKPVAEATTGEGSIDAPMPGTILDILVGVDQEVEAGQVVLILEAMKMEN
                     EIQSPLSGKVIQVNTTKGAAVNFGDSLLVIG"
     gene            3727..>4635
                     /locus_tag="VFD08_03750"
     CDS             3727..>4635
                     /locus_tag="VFD08_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HZK10166.1"
                     /translation="MLDVFINFIKSSAFVALDIKTIIMLVISFFLMYLAIVKKFEPLL
                     LIPISFGMLLANVPFAGMMDPPTENQFGGVLYYLYQGVELGVFPPLVFMGIGAMTDFG
                     PLISNPKSLFLGAAAQFGIFVTFIGAIASGAFTAAEGASIAIIGGANGPAALYLTSLL
                     APHLLGSIAIAAYSYMALVPVIQPPIMMALTSKEERMIKMKQMRVVSQKEKIIFPIMV
                     TLLVSLIVPASTPLVGMLMLGNLFKEALIVDKLRATASDAIMYTVTIFLGLTIGATAN
                     AEQFLSSKTILIMILGVVAFSFGTAMG"
BASE COUNT         1400 a          832 c         1099 g         1304 t
ORIGIN      
        1 atataaagaa ataagatgaa ttataaagaa gtaggatggg aaaacaaaag acaaattgcc
       61 aggatgatta gcatggttga aaatgcagct cctggtcatg aagagatttt tgaaaaaatc
      121 tatgataaaa cgggtcacgc ctatgttatt ggtattacag gccctcctgg ggcaggaaaa
      181 agttcactaa cagatcttct tgcaagaaac tatatttctc aagaaaaaaa agtgggcatt
      241 attgctgttg atcctaccag tcctttttca ggaggaagta ttcttgggga ccggataagg
      301 atgaatgagc taacgaccga cccaagagta tttattcgaa gtatgggaac ccggggacaa
      361 ctaggaggcc tttcagcagg gaccgcttct gcccttcaag tactagatgc agttggggcg
      421 gatataataa ttattgagac cgtaggtgtg ggtcaatcgg aagtggaaat aattaaatat
      481 gccgatagca tacttatggt tatggtgccg ggtctgggag atgatattca gaccatgaag
      541 gctggcatta tggaaatagg cgacgtattt gttgtcaata agagtgatct tcctggagct
      601 aataagacca agcttgagct agaagccatg cttagtttta tgcaaaacaa aaaatggaga
      661 ccacctgttc ttcttgccag tgcaagccgt aatgagggca ttaaagagct tgtagaggcc
      721 ctggataatc ataggaccta tcaagaaaaa gagggcctgc tctttgaacg aagaaagaga
      781 aatatcaatg aacttctatt tgaagtttta caagataaat tttttcgcca gataaaagaa
      841 ctggaggatt atcctcagct tttaaaagat ctaacaagaa aagaaatcga tccctacaca
      901 gcagcacagc tacttttaaa acaattaaag gagaaaagag gataaatgga aatacttaaa
      961 ttggaccata ttggaattgc agtaaaaagt ctagatgacg ccctaccctt ttggagagat
     1021 gttttgggtt taaaagaagc agggattgaa gtagtggaag aacaaaaggt aaaggttgct
     1081 tttttgcctg tgggagatac tgaagttgaa ctacttgaat ctacagatga agatgggcct
     1141 attgctaggt ttattgaaaa aaacggtcct ggcgttcaac accttgcctt tcgcgtaaag
     1201 gatatcaatg cagggattga ttatatgtta gaacagggca tgcgtatgat tgattcttct
     1261 gctcgctatg gagcgggagg agcccgcatt gcctttgtac acccgaaaag tagtcaagga
     1321 gttcttgttg agctctcaga aagagaagac tagagcattt tggaggagaa aatggataaa
     1381 aacaaactcc taattgataa aaaggagacg attattgctg gtggaggttc taagaggata
     1441 gaaaaacagc atgcctcagg aaaacttacc gcaagggaac gtttggccct cttgtttgat
     1501 aaagacacct ttattgagct ggatgccttt gtgagtcata gatgcacaaa ctttggtatg
     1561 gaaaaaatta gtgcaccagg agaaggggtc attacaggct atggctatgt ggatgatcgc
     1621 ttggtctatg cctatgccca agattttact gtaattgggg gaagccttgg tgaaatgcat
     1681 gcggaaaaaa tcattaaagc ccatgaaaat gccatgaagg taggggctcc ttttgtcggg
     1741 ctaaatgact ctggtggagc tagaattcaa gagggcgtag atgctctttc tggctacgca
     1801 aagatcttct atgctaatac tatggccagt ggggtaatcc ctcaaattac ggctattatg
     1861 ggcccctgtg ctggtggagc agtttattct cccgccttaa cagattttgt atttatggta
     1921 gatcaatcgg caaaaatgtt tattactggc ccccaggtta tagagactgt aacgggtgag
     1981 aaagtatcag ccgaagaatt aggcggatct atgacccata actctgtttc tggagtggca
     2041 cattttcata acaagagcga tgaggatacg cttgctcaga ttcgtaggct gattagtttt
     2101 cttcctagca ataatgctca aagggcgcca agcagggatc caggtgaggt ccaaggctat
     2161 ttagaagact tagaccatat tattcctgag agttcaagca agccctatga tatgcataaa
     2221 ataattgaaa gcttggtgga tcatggtgat tttatggaat accaagccta ttttgctaaa
     2281 aacattatta caggatttgc ccgcattggg ggaagaagcg tgggtattgt agccaaccag
     2341 ccccaggtgc tagctggaac cctagatgta aatgcttctg ataaggcagc ccgctttatc
     2401 cgtacctgtg atgcctttaa tattccactt ttaaccttgg ttgatgtacc aggtttttta
     2461 ccaggaactg accaggaata tgcaggaatt atccgccatg gagctaaaat gctgtattct
     2521 tatagcgagg ctacggttgc caaggtaacc ctgattgtta gaaaggccta tggaggttct
     2581 tatattgcca tgtgtaataa agaactagga gcagatgttg tttatgcttg gcctacagca
     2641 gaaatagctg ttatgggccc caagggagcg gcaaacatta ttttcagaaa agaaataagt
     2701 gcagctgaag atccagttgc tgaaagacaa gcccgtattg atcaatatga taaagaattt
     2761 gctaccccct ataaggcggc agaaagagga tatgttgatg atgtcatcct gcctcatagc
     2821 agccgggcaa gaattgtaga tgcctttgtt atgctagagg gtaagcgtgc tagtttaccc
     2881 cgtaaaaaac acggcaatat gcctttgtag gagataaaat gaaagaattt gcaagtatac
     2941 aagatatgtc ccagatgccg attatggaaa gacttagcaa tggtttgatt gtaacagttc
     3001 ttggtatggg aatgactatt ttggtcttgg ttctacttta ttttgccata aaactaatga
     3061 gcaatatcct tagtaaagaa gagtcgccaa aggccactca gagcgagccg attccccttg
     3121 caccccttaa agaagaaaaa gacaaagagg atgaggaact aatcgctgta tttatggcag
     3181 ctattatggc tcatacaggt caagatcata agaaaataaa atttcaaggc tatcgtgaag
     3241 taggaacaga tcttccttta tggagcaagg ccggtgttat ggaaacaatg aatagagaaa
     3301 tttaggagaa aagaatgaaa acatataaaa tcactgtaaa tggaacggtt tatgaagttg
     3361 atgtggaaga aattagagaa gatgcttcaa gctcagcccc ttcctatttg gcgcccagtt
     3421 ctgctcctag accagcaacc agtccagcac ctgcacctac acctacacca gtagctaagc
     3481 cggtagcaga agcgaccacg ggtgaaggaa gtattgacgc tcctatgcca ggaacaatct
     3541 tagatattct tgttggagtg gaccaggagg ttgaagctgg tcaggttgtt cttattttag
     3601 aggccatgaa aatggaaaat gaaattcagt cacccctttc aggaaaagtt attcaagtaa
     3661 atacgacaaa aggtgctgca gttaactttg gcgattcgct actggtaatt ggctaggagg
     3721 cctgttttgc tagatgtatt tataaacttt ataaagtcct cggcttttgt ggctttagat
     3781 atcaagacga ttattatgtt agtaatcagc ttttttctga tgtatctagc cattgttaaa
     3841 aagttcgaac ccctgcttct tattcctatt tcctttggaa tgctcttagc aaatgtcccc
     3901 tttgcaggaa tgatggatcc cccaacagaa aaccagtttg gtggcgtgct ctactatctt
     3961 tatcaagggg tagagttagg ggtctttccc ccccttgttt ttatgggcat aggtgcaatg
     4021 acagattttg ggcctttaat tagcaatccc aaaagtcttt ttttgggtgc tgctgctcaa
     4081 tttggaattt ttgttacttt tattggcgcc atagcatcag gagcctttac agcagctgag
     4141 ggagcttcaa tagctattat tgggggagca aatggtccag ctgcactcta tctaaccagt
     4201 ctattggctc ctcatcttct tgggtctatt gctatagcag cctattctta tatggcctta
     4261 gttcctgtta ttcagccacc cataatgatg gccttgacaa gcaaagaaga aagaatgata
     4321 aagatgaagc agatgcgagt ggtatcccaa aaagaaaaga ttatatttcc cattatggta
     4381 actctgcttg tttctttgat cgtgcctgcc tccacccccc tggttgggat gttgatgctg
     4441 ggaaatctgt ttaaagaagc ccttattgtt gataagcttc gggctacagc aagtgatgca
     4501 attatgtata ctgtaacaat ttttcttggt ttaaccattg gggctacggc taatgcagaa
     4561 caatttttat cttctaaaac aatcttgatt atgattttag gtgttgttgc attttccttt
     4621 ggaacagcga tgggc
//
LOCUS       DASRNA010000137        15439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1085723,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000137 DASRNA010000000
VERSION     DASRNA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15439
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085723"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..178
                     /locus_tag="VFD08_03755"
                     /pseudo
     CDS             53..178
                     /locus_tag="VFD08_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
     gene            398..1585
                     /locus_tag="VFD08_03760"
     CDS             398..1585
                     /locus_tag="VFD08_03760"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZK10167.1"
                     /translation="MGNTHELKYLQDKVDQLKEDGVYRKLPEMLGANGPVINLDGKKV
                     INLSSNNYLGFANHPRLKKAATEAIEKYGVGPGAVRTINGNMDIHEELERRLAHFKHE
                     EAVTVYQSGFNCNSGTIQAITDRGDLIISDELNHASIIDGVRLSRADKAVFKHSDMED
                     LERILKENQGKHKVTLIISDGVFSMDGDLAKLPEIVALAEKYGALTYIDDAHGSGVLG
                     ENGRGTVDHFGLHGRIDFIIGTLSKGMGALGGYVASKQVTKEWLSHRGRPILFSTSLP
                     PAIVAALIEAVNMLEESSEFTDRLWDNARYFKEELSKLGFDTGHSQTPITPVIIGDEA
                     KAVKFSKELLNRGVFASSIVFPTVPKGTARLRCMITAEHSKEDLKRAVELFDEVGKEM
                     GLI"
     gene            1554..2216
                     /gene="nth"
                     /locus_tag="VFD08_03765"
     CDS             1554..2216
                     /gene="nth"
                     /locus_tag="VFD08_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZK10168.1"
                     /translation="MKSVKRWDLFKLTKKVEALVEKLASLYPEARAELNYKNNFELLV
                     AVILSAQCTDVRVNIITKELFKEAPDPEAMVALGIEGITELIRSCGMYNQKAKNLYLT
                     SGLLLEKHGGQVPSSREALMELPGVGRKTANVVLSNAFGQPAIAVDTHVLRVSNRLGL
                     AKGKDPYKVEMQLQKILPKKIWTVMHHRLILHGRYCCKARNPQCQACGLLSLCPRIGV
                     DQ"
     gene            2213..2521
                     /locus_tag="VFD08_03770"
     CDS             2213..2521
                     /locus_tag="VFD08_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HZK10169.1"
                     /translation="MIISKNPYDPKIYRHMGFFFDSVVFTSSLQSDFAVLTKSFRQGN
                     IEEYEKMMSAEYEKFYQKIQEKLEAQGCDGLFNTTLHTEVLYAGTILLYASGDKVEKL
                     "
     gene            2518..2976
                     /locus_tag="VFD08_03775"
     CDS             2518..2976
                     /locus_tag="VFD08_03775"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HZK10170.1"
                     /translation="MIHIVLVEPEIPQNTGNIARTCALSGAKLHLVRPLGFSLEEKKL
                     KRAGLDYWDKLRLEVHTSLDAFLDKYGESKLYLITSKAEKTIYDSPLKGEVFFLFGKE
                     TAGLPEGLHKRYREHRLRIPMLEKEGLRSLNLSNSVAIVIYEAWRQGSYV"
     gene            3064..4269
                     /locus_tag="VFD08_03780"
     CDS             3064..4269
                     /locus_tag="VFD08_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HZK10171.1"
                     /translation="MKKFLALALVLLLSFSLISCGGQEAEEAPAPEATEEETVEEEAV
                     EEEVEEEAVEEEEEAEEAAEEVVADEETLIVDTEEVDRDSLKEITIGLVTDTGGIDDK
                     SFNQGSWEGVKYYAEKFELPEGNIGYLQSTSDADYIPNLAQYSEDKKDLIVAAGFLFE
                     ESLIKISEDFPEQKILFIDAVVDAPNVLNALFAEHEGSYLVGVAAALKAQDAGQDTVG
                     FVGGMDFDTIQKFEAGFEQGVLSVDPEMNIVVEYVGDFSAPEDGQTLAAKMYDSGVYV
                     IYHAAGGSGNGVIKEAKDRVQNGEDVWVIGVDKDQFSEGVYADGKSVILTSMLKGVGQ
                     ASYDASKQVAQDKFEGGTRIYDLKANGVGIPLVNPNLKDEWVEQINEFADKIINGEIE
                     VDPVPTRVQ"
     gene            4436..5959
                     /locus_tag="VFD08_03785"
     CDS             4436..5959
                     /locus_tag="VFD08_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010282299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10172.1"
                     /translation="MEQYVVEMRNITKRFPGILANDNVTLQLKKGEIHALLGENGAGK
                     STLMSVLFGFYKAEEGEIYVRGEKVNIKSPNVAHELGIGMVHQHFQLIDNFTVVENII
                     LGDEPGGILVDYAKARKQVEALSAQYGLGVDPDAKIEDISVGMQQRVEILKVLYRKAD
                     IIILDEPTAVLTPQEIDELVEIMKNFAAEGKSVILITHKLKEILIAADRVSVLRQGKY
                     VGTVNVKDTSEAAMAEMMVGRKVSFRVDKTPAKPGEVVLEIKDLWVKDHRNLDALRGL
                     NLQIRRGEILGIAGIDGNGQQELVEAITGLRKIDKGEILLQGNSIIDDSIRDHILKGI
                     AYIPEDRQKRALIMDFRLDENIVLKTYVNEPLSHGMLLDYREMQKKGKRIVSEFDVRS
                     SEGINTIVRNMSGGNQQKAIVGREMDLNPLVMVASQPSRGLDVGAIEYIHKRLVELRD
                     QGKAVLLISFELDEIMSVSDRINVIYQGRITGDVLSEETTIQELGLMMSGSKEVSLA"
     gene            5952..7046
                     /locus_tag="VFD08_03790"
     CDS             5952..7046
                     /locus_tag="VFD08_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10173.1"
                     /translation="MLKKRSHLTLSLIAVVFSFIVASLVMIISGVSPVTFFLSLFRTM
                     TGMNLKAIGEEGFFNARYLGEFLQMTMPIILTGLSVGFAFRTGLFNIGAEGQLIMGSV
                     GAVIVGVLFELPFFIHIPLAMLAATLFGALWGWVPGYLKAKFGVHEVVITIMMNYIAL
                     YLGNLMIKSLPGSTNQKSANLAPSALLHSDFLRGITGNSRFHWGFIAVIIAVIIYHFI
                     IEKTTFGYELRAVGFNRDAAEYGGIKVSSRIAYSMAIAGAFAGLAGAMLTLGTFDYGR
                     VLGSFENYGFDGIAVALLGGNTALGIFLGGLLFGGLKSSQTLMQASGIPLEIAIIVSA
                     LIILFVAMKNGIDMVLERMDARRRKKDESH"
     gene            7033..7983
                     /locus_tag="VFD08_03795"
     CDS             7033..7983
                     /locus_tag="VFD08_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10174.1"
                     /translation="MNLISSIVTLSLIYATPIIITALGGLYSERSGIVNIALEGIMVI
                     GAFVAATFTPLNEAALGAWTPWIAILLGAIMGCLYSLIHAFVSIDLQADQVISGTALN
                     MLSLGITVYFCQIIFNQQRTETFNIGFVKTNVPFFSEIPIIGDLFFKRMYYTFYLAIL
                     LVLVTAFILRRTRFGLRLKASGEHPSAVDSVGVSVRKMRYTGVLISGFLAGLSGGIMV
                     LTQATQFTAGSIHGVGFISLAALIFGRWNAYGVLGAGIFFGFSQIFAIFSKDIPLLAN
                     VPSEVFRALPYILTIIAILLFSRRSAGPKASGEIYDVSKR"
     gene            7992..9281
                     /gene="rpoN"
                     /locus_tag="VFD08_03800"
     CDS             7992..9281
                     /gene="rpoN"
                     /locus_tag="VFD08_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZK10175.1"
                     /translation="MHLDMSMVLEQKQQLILTPALKLSLDILQDNSIELKERVEKEVL
                     ENPLLEIRENSPPPRKSPSSYAPWDDLQERQSLSQYLLEQVRILELSSKERSLIERLI
                     EYISRKGYLLDEYQKDAFFLAFDKEEMEKALWILQGLKPAGIGARNLTECLVLQLRDL
                     GFDTSIALDIVSEDLEDVAAHRLEKLRKKYNIDLEKIEEAIRTIQSLDPKPGLAFHKS
                     DRIDSHIADVIIKKEEDQLDFELNEWTYPDVMVNPYYSSLSLDELDDKSKIYISNKQK
                     RAELFLEALSQRRKTISKVMKSIIDFQKKFFFIGEKGMIPLNLLDVAQEIDCHESTIS
                     RAINGKFLLFENQLYPLSYFFPSAVEGLEGGSVSSLRVKSILEEMVENENKEKPYSDS
                     ALAKKLEELGIYVARRTVAKYREELGILSSVLRKNFR"
     gene            9451..10458
                     /gene="gap"
                     /locus_tag="VFD08_03805"
     CDS             9451..10458
                     /gene="gap"
                     /locus_tag="VFD08_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZK10176.1"
                     /translation="MKNVAINGFGRIGQAAVRIWAQQKEKKFTLALINGGDVQRIYSS
                     LKYDSVYGTFPGTIEKGDKCVYVNGEKIEVRNQRDEDNLPWKEHKIDIVVDSTGRYRT
                     KEKAQIHLDQGAKKILVTAPVTGDDVTIVMGVNEDDYEHENHHIVSNASCTTNCLAPV
                     AKVLDREFGIEKGMMTTIHAYTGDQVLIDSKHKDPRRARAAALNIIPTTTGAAKAVAL
                     VLPQLEGKFSGMAVRVPTPTVSMVDLVITTKKPVTVEEVNQSLKTASEGEMKGLLGYS
                     EEPLVSSDYKADKRSSIVDALSTIVLGENMVKVIAWYDNEWGYAERVVDLCEYMVEKA
                     R"
     gene            10498..11679
                     /locus_tag="VFD08_03810"
     CDS             10498..11679
                     /locus_tag="VFD08_03810"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZK10177.1"
                     /translation="MKKTIRDIDVRGKKVFLRVDFNVPLKEGEIRDERRIVSSLPTIK
                     YLKEQGAKLILASHLGKPKGADPALSLGPIAKRLSDLLDSPVLFHTSSEVVDDKVRQM
                     VDQMKAGEILLLENTRYDVGETKNDPILSQKFASLADIFVDDAFGTAHRSHSSNYGVA
                     KLLPSRVSGFLIEKELKYFYEALENPKRPFLAILGGAKVSDKIKVIENLVERVDEIIV
                     GGAMAYTFMRAMGHEIGDSLVEEDQVDFAKKMLELAKEKEIPFHLPLDFATAPAYEDV
                     VPYYTKGRDIAPGTMGLDIGPQTRKAFVEVIDRSQTIVWNGPLGVFEMENFSAGTRAI
                     AEALSKNKGITIIGGGDSAAAVTEMGLDKNISHISTGGGASLELLEGKELPGIAVLEE
                     K"
     gene            11676..12416
                     /gene="tpiA"
                     /locus_tag="VFD08_03815"
     CDS             11676..12416
                     /gene="tpiA"
                     /locus_tag="VFD08_03815"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009529735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZK10178.1"
                     /translation="MRKEILAGNWKMNHGPKEALAFLKDFFVKPGENQRVILCPPYIS
                     LESMKRELPEKIELAAQNIHEEDKGAFTGEVSAPMIKELGVGITLLGHSERRNYYKES
                     DELVRQKLKKALEHNLEVILCVGEQLEHRESGRAEQVVKKQVLSALKGLKKEDMESVH
                     IAYEPVWAIGTGKTASSQDAQEMNAYIRSLIVELFNDKIAQETSILYGGSVKPENIKE
                     LMAMEDIDGALVGGASLDPISFAKLLDY"
     gene            12418..13914
                     /gene="gpmI"
                     /locus_tag="VFD08_03820"
     CDS             12418..13914
                     /gene="gpmI"
                     /locus_tag="VFD08_03820"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZK10179.1"
                     /translation="MKAALIILDGLGMGPPGEENAVYAANTPVLDGLFKKYPHTTLQA
                     SGVDVGLPQGQMGNSEVGHLNIGAGRIIYQPLLRIKRDMENRSFFKREVVREIFDSSL
                     EGKLHFLGLLSPGGVHSHSDHLKELLLEAKRKKIKDVYVHVFLDGRDTPPDSGLGEVE
                     DLVSFMEKEKIGKIASISGRYYAMDRDQRWERIQRAYDVLVYGKGELLEPIEGLKQSY
                     KQGLGDEFVIPFLSDPQGLINSGDRVVFFNFRPDRARELTQALTQKDFDEFNHEDLSL
                     RYYTMSQYSADFSNVKVIYPQELVENTLGEILSKEGKKQYRTAETEKYPHITFFFNGS
                     RESPFPGEDRLLVPSPKVATYDLMPEMSAYKVTRALVDKLKENTYDFYLVNFANPDMV
                     GHTGNFDATKKAVEAVDQCLGELVPLLEEKGIAFIITADHGNAEEMILPASGEVSTQH
                     SLNPVPFILGGLGELTLKEEGRLSDLAPTLLKILDLKQPKAMTGKAMF"
     gene            13923..15203
                     /gene="eno"
                     /locus_tag="VFD08_03825"
     CDS             13923..15203
                     /gene="eno"
                     /locus_tag="VFD08_03825"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZK10180.1"
                     /translation="MIIQGIYGREILDSRGNPTIEVEVYLDDGAMGRAQVPSGASVGQ
                     FEAVELRDGGKRYGGKGVQKAVENVNTTIAEALMGEYALDQAFIDKTLIELDGTSNKG
                     NLGANAMLGVSLAVAKAAANSLGIGLYQYLGGVGAKELPVPMMNILNGGSHADNNVDF
                     QEYMIMPVGAESFKEALMMGTAIYHELKNVLIKRGLSTGVGDEGGFAPNLSNNEEALE
                     VIEESVKALGLIPGEDVLYALDVAASEFYKDGNYHLASENRSLTSDELMDYYAELLEK
                     YPIVSIEDPFDEEDWEAFKKMTKRFAKKVQIVGDDLFVTNTQRLARGIEEKSANSILI
                     KLNQIGTITETLEAVEMAKRTRFTTVISHRSGETADSTIADFAVAVNSGQIKTGAPAR
                     SDRVEKYNQLLRIEEELGEASCYRGKDVFYNLDK"
BASE COUNT         4798 a         2680 c         3659 g         4302 t
ORIGIN      
        1 ccataatatt gggtatattc taatggacgc agagcttgag ccagacaagg agatggcaaa
       61 agactgtggc aagtgcaagg cgtgcattct ggcctgcccg caaggggcca ttggggacta
      121 ccaactagaa gctaaaaaat gcctttctta tattacccag tggaaagagg ccgaatatac
      181 accccttaaa ggatgttttt atggctgtga tatttgtttg agagcctgcc cctataatag
      241 ggtagaagaa agaaacaagg attttgtttt tgatgaaaac atatttttaa atttaagcaa
      301 tagagccttt aagcgccaat atggccatag ggactttgct tggattggaa aaaatagaat
      361 ttgccgaaat atcaattgga ttaaaaagga gaaaataatg ggaaatactc atgaactaaa
      421 gtatctgcaa gataaagtag accaactaaa agaagacggt gtttatagaa aactccctga
      481 aatgctgggc gctaatggac cagttattaa ccttgatggg aaaaaggtga ttaatctttc
      541 ttctaataac tatttgggct ttgccaatca tccgcgacta aaaaaagccg ctacagaagc
      601 tattgaaaaa tatggggttg ggccaggagc ggttagaacc attaatggaa atatggatat
      661 tcatgaagag ctagaaagac gcttggctca ttttaagcat gaggaagcgg taaccgtcta
      721 tcaatccggt tttaactgca atagcggaac catccaagcc attacagacc gaggcgattt
      781 aattatttcc gatgaactca atcacgcttc catcattgat ggggtccgcc tttcccgagc
      841 agacaaggct gtctttaagc attctgatat ggaagacttg gagagaatac taaaagaaaa
      901 ccaaggcaaa cacaaggtta ctcttattat tagcgatggc gtttttagta tggatggcga
      961 tttggccaag ctaccagaaa ttgttgcctt agcagaaaaa tatggtgcac tcacctatat
     1021 tgatgatgcc catggttctg gagtgttagg cgaaaatggc cggggaaccg tagatcattt
     1081 cggccttcat ggtcgcatag attttattat tggaacccta tccaagggca tgggtgcttt
     1141 aggtggatat gtagctagta agcaggtaac aaaagaatgg ctatcccatc gaggacgtcc
     1201 tattcttttt agtacaagtt tgcctcctgc tatagtggcg gccctaattg aagcagtcaa
     1261 tatgctagaa gagtcttcag agtttaccga tagactgtgg gacaatgctc gttactttaa
     1321 ggaagaatta agtaagcttg gctttgatac aggccacagc cagacaccca ttactcctgt
     1381 tataatcgga gatgaagcca aggcagtgaa gttctccaaa gaacttctta accgaggtgt
     1441 ttttgctagt tccattgtat ttcccactgt acccaagggt actgccagac tgcgctgcat
     1501 gattacagcg gagcatagca aggaagatct taaacgtgca gtagaactat ttgatgaagt
     1561 cggtaaagag atgggactta tttaaattga caaaaaaagt tgaggcacta gttgagaaac
     1621 tggctagctt atatcctgag gcaagggcag aattgaacta taaaaacaat tttgagcttc
     1681 ttgtggcagt gattctttca gctcagtgca ctgatgtacg cgtaaatata ataaccaagg
     1741 aactttttaa agaggctccc gacccagaag ctatggttgc actaggcatt gaagggatta
     1801 ctgagcttat tcgttcctgc ggtatgtata atcaaaaggc aaaaaaccta tatttgactt
     1861 ctggactcct attagaaaaa cacgggggtc aagttccttc tagtcgtgag gctttgatgg
     1921 agcttccggg ggtgggacgc aaaacagcaa atgtggtttt aagcaacgcc tttggccagc
     1981 cagccattgc ggtggacacc catgttctta gggtatccaa tcgcttgggc ttggccaagg
     2041 gaaaggatcc ctataaggta gaaatgcagt tgcaaaaaat actgccaaaa aaaatatgga
     2101 ctgtaatgca tcaccgcctt attttacacg gacgttactg ttgtaaggcc cgcaatcctc
     2161 agtgtcaggc ctgtggcctc ttatctcttt gcccgcgcat aggagtcgat caatgattat
     2221 atctaaaaat ccatacgatc caaagattta taggcatatg gggtttttct ttgatagcgt
     2281 agtttttacc agctctttgc agagtgattt tgctgttctt actaaaagct ttcgccaggg
     2341 gaatatagaa gagtatgaaa agatgatgag tgcggagtat gagaagttct atcaaaaaat
     2401 acaagagaaa ttggaagctc agggttgtga tggtcttttt aatacaactc ttcacaccga
     2461 ggtcctatat gcaggtacaa tcttactcta tgcttcgggg gacaaagtag aaaaactatg
     2521 attcatattg ttcttgttga accagaaatc ccacaaaata caggcaatat tgctaggact
     2581 tgtgccttaa gtggggccaa actccatcta gtaagacctc taggtttttc tttagaagaa
     2641 aaaaagctca aaagagcagg tcttgattat tgggataaac tgaggctgga agttcatact
     2701 agcctagatg cttttttgga taaatatggt gagagcaagc tatatcttat tactagcaag
     2761 gcagaaaaaa caatttatga ctctcctctt aagggagaag tgttttttct ttttggaaaa
     2821 gagactgctg ggttgccaga aggtcttcac aaaagatata gggagcatcg acttcgcatt
     2881 cccatgcttg aaaaagaggg actgcgttca cttaacttat ctaactccgt agccattgtt
     2941 atttatgagg cttggcgaca aggaagctat gtctaaatgt ttaacttact ggtaaatggg
     3001 taaatattcc cattgaggac acaaaatata tcccggtgag gatattattt taggaggtat
     3061 ttaatgaaga aatttttagc actagctctg gtactgttac ttagcttctc gcttatttct
     3121 tgtggtggac aagaagccga agaagcgcca gcaccagagg cgactgagga agaaactgtc
     3181 gaagaagaag ctgtcgaaga agaagtggaa gaagaagctg tcgaagaaga agaagaagcg
     3241 gaagaagcag cagaagaagt ggttgctgat gaagaaacgc tgattgttga cacagaagaa
     3301 gttgacaggg attcactaaa ggaaatcacc atcggtcttg ttaccgatac tggtggaatt
     3361 gatgacaagt catttaacca aggttcatgg gaaggcgtca agtactatgc cgagaaattt
     3421 gaacttcctg aaggaaatat aggctattta caaagtactt ccgatgccga ttatattcca
     3481 aacctagccc agtacagtga agacaaaaag gatctgattg ttgcagctgg attcctcttt
     3541 gaagaatctt tgattaagat ttctgaagac ttccctgagc aaaaaatact atttattgat
     3601 gctgtggtag atgctcctaa tgttcttaat gcgctttttg ctgaacatga aggttcttat
     3661 cttgtaggtg ttgctgctgc tcttaaggcg caagacgctg ggcaagatac agtaggcttt
     3721 gttggtggca tggactttga tacaattcaa aagtttgaag ctggctttga gcaaggtgtt
     3781 ctatcagttg atccagaaat gaatattgtt gttgaatatg ttggtgactt cagtgctcct
     3841 gaagatggac aaacactggc agcaaaaatg tatgattctg gtgtttatgt aatttatcat
     3901 gcagctggtg gatcaggaaa tggtgttatt aaagaagcca aagaccgtgt tcaaaatggc
     3961 gaggatgtct gggtaattgg tgttgataaa gatcaattct ctgaaggtgt ctatgctgat
     4021 ggaaaatctg ttatactcac atctatgctt aaaggtgtag gacaagcatc ctatgatgct
     4081 agtaagcaag ttgcccaaga taaatttgag ggtggaactc gcatctatga ccttaaggcc
     4141 aatggggtag gaattccttt ggtaaatcca aatcttaaag atgagtgggt agagcaaata
     4201 aacgaattcg ctgataaaat cataaatggc gaaatcgaag tagaccctgt tccaactcgc
     4261 gtacagtaat atatttctag taaagaaagg cccttggcta taaaaagcct ggggcttttt
     4321 ttctgtcttt ttttagattt gttcccctat aaaaacagtg aatttacggc ctccaattaa
     4381 caaaaaaaag cttaatcatg gtatactggc cttaataaga tagaaagggg agaaaatgga
     4441 gcaatatgta gttgaaatgc gaaatattac taagcgtttt cccgggatcc ttgccaatga
     4501 caatgtgact ttacagctaa aaaaaggcga gattcatgcc ctcctaggtg aaaatggtgc
     4561 tggtaaatcg actttaatgt cggttctttt tggtttttac aaagcagaag aaggtgaaat
     4621 ttatgtaagg ggagaaaagg taaatataaa atctcccaat gtagctcatg aactgggtat
     4681 tggtatggtt catcagcatt tccagttgat tgataatttt actgtagttg aaaacattat
     4741 tctgggcgat gagcccggag ggatcttagt tgactatgcc aaggcaagaa aacaagttga
     4801 agccctttcg gctcaatatg gtttgggagt agaccccgat gccaagattg aagatattag
     4861 tgttggaatg cagcagagag tagagatcct taaggtactt tatagaaaag ccgatattat
     4921 aatattagat gagcccactg ctgttttaac accacaagaa attgatgaac ttgtggagat
     4981 tatgaaaaac tttgcggcag agggaaagag tgttatttta attactcata aactaaagga
     5041 aatccttatt gccgcagata gggtaagtgt tcttcggcaa ggaaagtatg ttggaacagt
     5101 aaatgtgaaa gataccagcg aagcagccat ggctgagatg atggtaggac gcaaggtaag
     5161 ctttcgtgtt gataagaccc ctgccaaacc aggggaggtt gtgctagaaa ttaaggacct
     5221 ttgggtaaaa gaccatcgaa atttagatgc ccttcgcggt ttaaacttac aaattcgccg
     5281 gggtgaaatt ttgggtattg ctggaattga cggcaatggc cagcaagaac tagttgaagc
     5341 aattacgggt ctgcgcaaaa tagataaagg agaaatatta ctccagggca actctatcat
     5401 tgatgatagt attcgagatc acattctaaa ggggattgcc tatatcccag aagaccggca
     5461 gaaaagagcc cttataatgg actttagatt ggatgagaat attgttctta agacctatgt
     5521 aaacgagccc cttagccatg gaatgctact tgattatagg gagatgcaaa aaaaaggaaa
     5581 acgcattgtc agtgagtttg atgtgcgaag ctctgaagga atcaatacca tcgtgcgcaa
     5641 tatgagtggg ggcaaccagc aaaaggccat cgttggaaga gaaatggatt tgaatccact
     5701 ggttatggtc gcctctcagc cttctagagg tcttgatgtt ggagcaattg aatatataca
     5761 taagcgtctg gtagaactaa gagatcaagg caaggctgtt ctgcttatca gctttgaact
     5821 cgatgaaatt atgagcgtaa gtgatcgaat caatgtaatt tatcaggggc gaatcactgg
     5881 tgatgtcctt tctgaggaaa caacaattca agagttggga cttatgatga gcggatcaaa
     5941 ggaggtttcc cttgcttaaa aaaaggagtc atcttacttt aagtttaatt gctgtagttt
     6001 tttcttttat tgtcgccagt ttggttatga taatttcagg agttagtcct gtcacctttt
     6061 tcctatccct ctttcgtaca atgacaggaa tgaatttaaa agcgataggt gaagaaggtt
     6121 tttttaacgc gcgctatctg ggtgagtttc tccaaatgac catgcccatt attttaacgg
     6181 gactttctgt tggttttgcc tttagaaccg gtctttttaa tattggtgcg gaaggtcaat
     6241 taattatggg aagtgtggga gcggtgattg tgggtgtctt gtttgaattg ccctttttta
     6301 ttcatatccc cttggccatg cttgcagcca ccttgtttgg agccctttgg ggatgggtgc
     6361 ctggttatct taaggcgaag tttggggttc atgaagttgt tataacaatt atgatgaact
     6421 atatagccct ttacctgggg aatttaatga ttaagtccct tccaggaagc accaaccaaa
     6481 aaagtgcaaa cttagctccc tctgctcttt tacatagtga cttccttcgg ggcataacgg
     6541 ggaactcccg ttttcattgg ggttttatcg ctgtgattat agcagttatt atctatcact
     6601 ttattattga aaaaacgacc tttgggtatg agcttagggc ggtgggtttt aatcgagatg
     6661 cagccgaata tggtggcatt aaggtttcaa gtcgcattgc ctattctatg gccatagcag
     6721 gagcctttgc aggacttgct ggtgctatgc tgacccttgg aacctttgac tatggtcgtg
     6781 tacttggttc ttttgagaat tatggttttg atgggattgc tgtagccctt ttgggaggaa
     6841 atacagcttt gggaattttc ttgggtggtt tgctctttgg tggattaaaa agctcccaga
     6901 ccttgatgca ggcttctgga atacctttgg agattgctat tatagtttca gcccttatta
     6961 tcctttttgt ggctatgaaa aatggcattg acatggtgct tgaacggatg gatgcaagaa
     7021 ggaggaagaa agatgaatct cattagttca attgtgaccc tatcgcttat ttatgctacc
     7081 cctatcatca ttaccgccct gggtggtctt tatagcgagc gaagtggtat tgttaatatt
     7141 gcccttgaag ggataatggt aataggtgcc tttgtggccg ctacctttac gccacttaat
     7201 gaggccgctc taggggcctg gactccttgg attgctattt tgctaggggc tataatggga
     7261 tgtctttata gtctgattca tgcttttgtt agtattgacc tgcaagcaga tcaggtgatt
     7321 tctggtaccg cccttaatat gctttcccta ggaattactg tttatttttg tcagattatt
     7381 tttaatcagc agagaacaga aacctttaat ataggctttg taaaaacaaa tgtacccttt
     7441 ttctcagaga ttcccattat tggagatcta ttctttaaga ggatgtatta cactttttat
     7501 ttggccatcc tgctggtttt agttaccgcc tttatcctta ggaggactcg ttttggtttg
     7561 cgccttaaag caagtgggga acatccttcg gcagtggata gtgttggtgt aagtgttcga
     7621 aaaatgcgtt atacgggtgt cttgatttct gggtttttag caggactttc tggcgggatt
     7681 atggttctta cccaggcgac gcaatttacc gcaggaagta ttcacggggt aggttttatc
     7741 tccctagctg cccttatctt cgggcgctgg aatgcctatg gcgttttggg agccggtata
     7801 tttttcggtt tttctcaaat ttttgctatc tttagtaaag atatccctct tttggctaat
     7861 gttccctcag aggtttttcg tgccctacct tatattttaa caattattgc cattctacta
     7921 tttagccgta gaagtgcagg acccaaagca tcaggggaaa tatatgatgt atcaaaacgc
     7981 taggagactt tatgcatctt gatatgagta tggtgttaga acaaaagcaa caattaattc
     8041 taacaccagc cctaaaactt agtttggata tccttcagga taattcaata gaactaaagg
     8101 aaagggttga aaaagaagtg ttagaaaacc ctctgcttga aatacgcgaa aacagtcctc
     8161 ctcccagaaa aagtcctagt agctatgctc cctgggatga cttgcaggag cgccaaagtc
     8221 ttagccagta ccttcttgag caagttagga tattagaact tagctcaaaa gaacgcagtc
     8281 tgatagaaag actgattgaa tatatcagcc gcaaaggcta tctcttagat gagtatcaaa
     8341 aggatgcttt ctttttggcc tttgataaag aagaaatgga aaaggcccta tggattttac
     8401 aagggctaaa accagcagga attggggctc gtaatttaac agagtgtctg gtcttgcagt
     8461 taagagattt gggttttgat acctctattg ccctagatat tgtaagtgag gatcttgaag
     8521 atgtggcagc ccatcgccta gaaaaactaa ggaaaaaata caatatcgac cttgaaaaaa
     8581 tagaagaggc aatccgaacc attcagtctt tggatccaaa gccagggctt gcctttcata
     8641 aaagcgatcg aattgactct catattgccg atgtaattat taaaaaggaa gaagatcaac
     8701 tggactttga actaaatgaa tggacctatc cagatgttat ggtaaatccc tactactcca
     8761 gccttagtct tgatgagcta gatgataaaa gcaaaatcta tatttctaat aagcagaagc
     8821 gcgcagagtt gtttttagaa gcccttagtc aaagacgaaa aaccattagc aaggtaatga
     8881 aaagcattat agattttcaa aaaaaattct tttttatcgg cgaaaagggc atgattcctc
     8941 ttaatcttct tgatgttgcc caagagatag attgtcatga atccacaatt tcccgggcca
     9001 tcaatgggaa gtttcttctt tttgagaatc agctatatcc tttgagctat tttttcccta
     9061 gtgctgtaga gggacttgag ggtggcagtg taagttccct tagagtaaag agtatcttgg
     9121 aagaaatggt agaaaatgag aacaaagaaa aaccctatag tgatagtgcc ttggcaaaaa
     9181 agctagagga actgggaatc tatgtggccc gaagaacagt ggccaagtac agagaagagc
     9241 taggaatttt gtcttctgtt ctaagaaaaa actttagata gattttaaaa ggagtcttta
     9301 gtgggtatat aagccagtag gtgttactat caacttactt taaacaaaac caaataagca
     9361 attatccttg ttggcaagag ggatggaagt gctttttata aaaacagatt tactaactta
     9421 taaaaaaaag aagaaaagaa aggaccaaaa atgaaaaacg tagcaataaa tggatttgga
     9481 cgtataggac aagccgccgt acgtatttgg gctcagcaaa aagaaaagaa gtttactcta
     9541 gctctgataa acggaggaga tgttcagcgt atctattcgt ctttaaagta tgattctgta
     9601 tatggcactt ttcctggaac aatagaaaag ggagacaagt gtgtttatgt aaatggcgaa
     9661 aagatagaag tacgcaacca aagagacgaa gataatcttc cttggaaaga acataagata
     9721 gatatagttg ttgattctac aggtcgctat agaacaaaag aaaaagcaca aatccacttg
     9781 gaccaaggag caaaaaaaat cctggttact gcaccggtta caggagatga tgtaaccatt
     9841 gttatgggtg taaatgaaga tgactacgag catgaaaatc accatattgt atcaaatgca
     9901 tcttgtacaa ccaactgtct agcacctgtg gccaaggtct tggatagaga gtttggaatt
     9961 gaaaagggca tgatgaccac aatacatgct tatacgggtg atcaagtatt aatcgatagc
    10021 aagcataagg atcctcgcag ggcccgagct gctgctttaa atataatccc caccaccaca
    10081 ggagccgcca aggctgttgc cctggttctt cctcaactag agggaaagtt ttcaggaatg
    10141 gctgttagag ttcctactcc tactgtttct atggtggatc tagttattac aaccaaaaaa
    10201 ccagtaacag tagaagaagt gaatcagtca cttaaaacag caagtgaagg tgaaatgaag
    10261 ggtcttcttg gttatagtga agagccccta gtatcatcgg actataaagc ggacaagcgc
    10321 tcttctatag tagatgcttt atccactatt gttttgggtg agaatatggt aaaagttata
    10381 gcatggtatg acaatgaatg gggctatgct gagcgtgtag ttgacctttg tgaatatatg
    10441 gtagaaaaag caagataaga agatcccgac ttaggtcggg atttttaagg aggacctatg
    10501 aaaaagacaa ttcgagatat agatgttagg ggtaagaaag tatttcttcg agtggacttt
    10561 aatgtaccac taaaagaagg ggagattcgt gatgagagaa ggatcgtttc gtcgcttcct
    10621 acaatcaaat atttaaagga gcagggggca aaactaattt tagcctctca tttaggaaaa
    10681 ccaaaagggg ccgaccctgc acttagccta ggacccattg ccaagcgcct aagtgacttg
    10741 ttggacagtc cagtactttt tcacactagt agtgaagtag tggatgacaa agtaaggcag
    10801 atggtagatc agatgaaagc aggggagatc cttcttcttg aaaacacccg ttatgacgtg
    10861 ggagaaacca aaaacgaccc tattctttcc cagaagtttg cctctcttgc agatatcttt
    10921 gttgatgacg cctttggaac agctcatagg tcccatagtt ctaattatgg ggtagccaag
    10981 cttcttccta gcagggtgtc tggattttta atagaaaaag agctcaagta tttttatgag
    11041 gctcttgaaa atcccaaaag gccatttttg gctattttag ggggagccaa ggtatcagac
    11101 aaaataaaag taatagaaaa cctagttgaa agagtcgatg agattattgt tgggggagcc
    11161 atggcctata cttttatgag ggctatgggg catgaaattg gtgactctct tgttgaggaa
    11221 gaccaggttg attttgcaaa aaaaatgtta gaacttgcca aggaaaaaga aattcctttc
    11281 catcttcccc tggactttgc tacggcgcca gcctatgaag atgttgttcc ttattataca
    11341 aagggcagag atattgcgcc tggcacaatg ggtcttgata ttggccccca gactagaaaa
    11401 gcatttgttg aagttataga cagatctcag accattgttt ggaatggtcc actgggcgtt
    11461 tttgagatgg aaaacttttc tgcaggaaca agggccattg ctgaggctct atcaaaaaac
    11521 aagggcatta caattatagg tggtggcgat agtgctgctg ctgttactga aatgggcctt
    11581 gataaaaaca taagccatat ttctactggg ggtggggcga gcctggaact tttagaagga
    11641 aaagaactcc ctggtattgc agtattggag gaaaaatgag aaaagaaatt cttgcaggaa
    11701 actggaaaat gaatcatggt cccaaagaag ccttggcttt tctgaaagac ttttttgtta
    11761 agccagggga aaatcagcgg gtaatcttgt gtccacccta tatttctttg gagagtatga
    11821 aaagagagct tccagaaaaa atagagttag ctgctcaaaa tatccacgaa gaagataagg
    11881 gagcctttac tggtgaagtt tcagctccaa tgataaaaga actgggcgtt ggaattaccc
    11941 tcttaggcca ttctgagcgc agaaactact ataaggaaag cgatgaactt gttcgccaaa
    12001 aactcaagaa agccctagaa cacaatctag aggttattct ttgtgttgga gagcagctcg
    12061 agcatagaga atcaggccgg gcagagcaag tggttaaaaa acaagtttta tcggccctta
    12121 aaggtctaaa aaaagaagat atggaatcgg tccatattgc ctatgaaccg gtttgggcca
    12181 ttgggacagg aaaaacagca tctagtcaag atgcccaaga aatgaacgct tacatcagaa
    12241 gtttaatcgt agaacttttt aatgataaaa ttgcccaaga aacctccatt ctctatggag
    12301 gcagtgtaaa gcccgaaaat atcaaagaac taatggctat ggaagatatt gatggggccc
    12361 tggtgggagg ggctagttta gatcccatta gctttgccaa gctcttggac tactaatatg
    12421 aaagcggcac tgattatctt agatggactg ggaatgggtc cgcccgggga agaaaacgct
    12481 gtctatgcgg caaatacacc agttctagat ggactcttta aaaaatatcc tcatactact
    12541 cttcaggcat ccggagtaga tgtgggactg ccccaggggc agatgggcaa ctccgaagtg
    12601 ggccatttaa atattggggc aggaagaatt atctatcaac ctcttcttag aatcaaaaga
    12661 gatatggaaa acagaagttt ttttaagagg gaagtggtaa gggaaatctt tgactcctcc
    12721 cttgagggaa aattacattt tttagggctt ttgtctcctg gtggcgtgca ctctcatagt
    12781 gaccatttga aggagcttct tttagaagcc aaaagaaaaa agatcaagga tgtttatgtt
    12841 catgtttttc ttgatggtag ggataccccc cctgatagtg ggctaggtga ggtagaggac
    12901 ctagtaagct ttatggaaaa agaaaaaata ggaaaaatcg cctctattag tggccgttac
    12961 tacgccatgg atcgcgacca aagatgggaa agaatacaaa gggcatacga tgtcctggtt
    13021 tatggaaaag gagagctttt agagccaatt gagggcctaa aacaaagcta taaacaagga
    13081 ctaggggatg aattcgttat tccttttcta agcgaccccc agggcctaat taattcgggg
    13141 gaccgagtgg tgttttttaa ctttcgacct gatagggcaa gagaactcac tcaggcctta
    13201 actcagaagg actttgatga atttaatcac gaagatttgt cactaaggta ctatactatg
    13261 agccaatact cagcagactt tagtaatgtc aaggtaatct accctcaaga attagtagaa
    13321 aatactttgg gagaaatact ctcaaaggag ggtaaaaagc aataccgcac ggcagaaacg
    13381 gaaaaatacc cccatattac ttttttcttt aatggcagta gagaaagtcc ctttccagga
    13441 gaagaccgtc ttcttgtgcc ttctcctaag gtggcgacct atgatcttat gcccgagatg
    13501 agtgcctaca aggtgaccag ggccctggta gacaaattaa aagaaaatac ttatgatttt
    13561 tatttggtaa actttgccaa tccagatatg gtgggtcata caggaaattt tgatgcgaca
    13621 aaaaaagcag ttgaagcagt ggaccaatgt ttgggtgaac ttgtgcccct gctagaagaa
    13681 aagggaatcg cttttattat taccgcagat catggcaatg ctgaagaaat gattttgcca
    13741 gccagtgggg aagtatcaac ccagcacagt ttaaaccctg ttccctttat attagggggc
    13801 ctgggagaac ttacattaaa agaagaagga agactttctg atctggcccc gactctttta
    13861 aaaatattag atttaaaaca accaaaggcc atgactggca aggcaatgtt ttaggaggaa
    13921 aaatgataat tcaaggaatt tatggaagag aaattttaga ctctagggga aatcctacta
    13981 ttgaggtgga agtttatttg gacgatgggg ccatgggtag agctcaagtg cccagcggag
    14041 caagtgttgg acagtttgaa gcagtagaac ttagagatgg tggcaaacgc tatggtggaa
    14101 aaggcgtgca aaaagcagtg gagaacgtca atacaactat tgcagaggcc ctaatgggcg
    14161 aatatgcgct ggatcaggcc tttattgata aaacattaat tgagcttgat ggcacaagca
    14221 ataaaggaaa cctaggagcc aatgccatgc taggagtttc tcttgctgtt gccaaggccg
    14281 cagcaaatag cctaggaatt ggtctctacc aatatttggg tggtgtagga gccaaagagc
    14341 tccctgtgcc aatgatgaat atcctaaatg gaggaagtca cgctgataat aatgtggact
    14401 tccaggagta tatgattatg cctgtagggg cggagagttt taaagaagcc ttgatgatgg
    14461 gaacggctat ctatcatgaa ctgaaaaatg ttctaataaa aagaggtcta tccactgggg
    14521 ttggcgatga gggcggattt gctcccaatc tctccaacaa tgaagaggct ttagaagtga
    14581 ttgaagaatc agtaaaagcc ctgggactaa ttcctggaga ggatgttctt tatgctcttg
    14641 atgtagcagc cagtgagttt tataaggatg gcaactacca tctagcaagt gaaaacagga
    14701 gtttgactag tgatgagctg atggattatt atgctgaact tctagaaaaa tatcccattg
    14761 tttctatcga ggatcccttt gatgaggaag actgggaagc ttttaaaaag atgacgaaaa
    14821 gatttgcaaa gaaggtgcag attgtcggag acgatctatt tgtaaccaac acccagaggc
    14881 tggcccgagg gattgaagaa aaatcagcta attctatcct cattaagtta aatcaaatag
    14941 gaaccattac ggagacccta gaagccgtag aaatggcaaa aagaacgaga tttacaacag
    15001 ttataagcca tcgtagtgga gaaactgctg atagtacaat tgctgacttt gctgtggctg
    15061 ttaatagtgg gcagattaaa acaggggccc ccgcccgctc tgatagagtg gaaaagtaca
    15121 atcagcttct acgcatagaa gaggaactag gcgaagccag ttgctataga ggaaaagacg
    15181 tattctataa tctagacaaa taagagattt tatgatagct gccgagaagg acaaaaaaac
    15241 ttgtctttct cgtttttttt actttaaaag aaaatgaaag aaggaagaag tcttatcaag
    15301 atcaacttcc aggaaccatt ttaggcgtaa gcatcaagaa aaagaagaga agaaaatcag
    15361 aacatcttga aaatttaggc tgatttattt gtgaggtaag atggaaaaaa aataaagtac
    15421 taaaaaaggc gaaaactta
//
LOCUS       DASRNA010000138         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_116297,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000138 DASRNA010000000
VERSION     DASRNA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116297"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFD08_03830"
     CDS             <1..755
                     /locus_tag="VFD08_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZK10181.1"
                     /translation="VAYTGGLNIADEYINKKKRFGYWKDTAVKIQGDAVDSFTVMFLE
                     VWNVLEGGHLEERYLRESKSSLAKDGFVIPYYDTPYNGEEIGKKVYLDIIHQAQESIY
                     IMTPYFIIDYEILYALCYSAKSGVDVKIILPHIPDKRWIFYTSRTFYGELLRAGVEVY
                     EYLPGFLHAKSFASDGNKAVVGTVNMDYRSFYLHLECGAYFYNTPVSKELEEDFIETL
                     GVSQKITMDIVEDFPFYQRMIGRLMRLIAPLL"
     gene            complement(820..>3229)
                     /locus_tag="VFD08_03835"
     CDS             complement(820..>3229)
                     /locus_tag="VFD08_03835"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZK10182.1"
                     /translation="IAALVSFGIAIYQGQSKSFAEPLLILLIVIMNAIMGTLQEAKAE
                     KALEALKSLTSLQARVFRDGDEQVMDSSQLVPGDIIHLEAGDFVPADARLIESSSLKS
                     DESALTGESVPVEKDASLEIEDKVPLGDRLNMVYSGCPISYGTARALVTHTAMNTQIG
                     KIAQLLDSEADTQTPLQQKLGKLGKNLGFIALAACALVFIIGIIDNLPPMVIFMTSVS
                     LAVSVIPEGLPAVVTIVLSLGIQRMVKKHAIIRKLPAVETLGSASVICSDKTGTLTMN
                     QMTLLEGYAVGEKEESIEGEISSELTQALSYATLCSNGSVREEEGEILYLGDPTETAL
                     IYAAHIKGQRKEALEKEFPRIYELPFDSERKRMSTIHKTDKGYLVITKGAFDMLEGLS
                     IKTDLSQARAISDKMSSQALRVLALGIKYLDELPENITSEEIEKDLELTALFGMIDPP
                     REEAKVAVQQCRLAGIKPIMITGDHILTAKAIAKQIGIFQEGDEAITGSDLDNLSDQQ
                     LEEEIRKISVYARVSPENKIRIVKAWQSLGEVVAMTGDGVNDAPALKAADIGCAMGIT
                     GTDVAKNAADMTLTDDNFATIVDAVEEGRGIYSNIRKVVGFLLGCNVGELFSVLIAML
                     VWKDTPLLSMHLLWINLVTDGPPALALGVEEVEKDVMKQKPVKRDENIFARGLGLRIA
                     LQGGMFTILTLSAFYLGKNIGGDITTARTMAFLVLATSQLVHSFNMRSQHSLFEIGFF
                     TNKSLNLAVLLSAGLMLLVLFPLHELFQITLLTPRLYLYGLGLSLVPLVFEEIRKQIF
                     KKVI"
BASE COUNT          859 a          685 c          745 g          940 t
ORIGIN      
        1 aagtagccta tacgggggga ctaaatattg cagatgagta tattaataag aagaagcgtt
       61 ttggctactg gaaggatacg gccgtaaaaa tacaaggaga cgcagtagat agttttacag
      121 ttatgtttct tgaagtttgg aatgtactag aagggggcca tctagaagag cgatatctta
      181 gagaaagcaa aagctctcta gctaaggatg gttttgtcat tccctattat gataccccct
      241 ataatgggga ggaaattgga aaaaaagtct atctggatat tatccaccag gcccaggagt
      301 ctatttacat tatgacccct tactttatta tcgattatga aatcctctat gccctttgct
      361 actcagctaa aagtggggta gatgttaaga ttatcctgcc tcatattcct gataagcgct
      421 ggatttttta cacctcccgg accttttatg gggaacttct tagggctggg gtggaggttt
      481 atgagtatct gccgggcttt cttcatgcca agagttttgc ctccgatggc aataaggctg
      541 tagtgggaac ggtaaatatg gactatagaa gtttttatct gcatctggag tgtggggctt
      601 atttttataa tacgccagta agtaaggagc tagaagaaga ttttattgaa accctgggtg
      661 tatcacaaaa gattaccatg gatattgtgg aggactttcc tttttatcaa aggatgattg
      721 gaaggcttat gcgccttatt gcccccctgc tctaaaactt tactgaggac ttactaagat
      781 aaaaaaaacg gctaggcctt tgcctaaccg tttttttgat taaatgactt ttttaaagat
      841 ttgttttcta atctcctcaa agacaagggg aacaaggcta aggcccaggc cgtagagata
      901 aagtcttggt gtaagaaggg taatctgaaa gagctcatgt agaggaaaga gtacaagcag
      961 cataaggcca gcgcttaata aaacagcaag gtttaggctc ttattggtga aaaaaccgat
     1021 ttcaaaaaga gaatgctgac ttctcatatt gaagctatgg acaagctgac ttgtagctag
     1081 cacaagaaag gccatggttc ttgctgtggt tatatctccc cctatgtttt tgccaagata
     1141 aaaggcgctt agggttagga tggtaaacat gcccccttgt agggcaatac gcaggccaag
     1201 gccccgggca aagatatttt catctcgctt aacgggtttt tgcttcatta catctttttc
     1261 tacctcttca actcctaggg ccagagcagg aggaccatcg gtaacaagat tgatccacag
     1321 tagatgcata cttagcaggg gagtgtcttt ccagaccagc atggcaataa gaacactaaa
     1381 gagttctcct acattgcagc caagaagaaa tcctactact ttacgaatat tggagtagat
     1441 tccccttcct tcttctacgg cgtctacgat ggtggcaaag ttatcatcag ttagggtcat
     1501 atcggcagca ttttttgcta catctgtgcc ggtaatgccc atggcgcatc caatatcagc
     1561 ggcttttagg gcgggggcgt cattgactcc atcgcctgtc atagcaacaa cttctcccag
     1621 actttgccaa gccttgacaa ttcgaatttt attttctggg gagactctgg catatacaga
     1681 gatctttcgg atttcttctt ctaactgctg atcactaaga ttatcaaggt ctgaacctgt
     1741 aatggcctca tcgccctcct ggaagatacc gatttgtttg gcaatggctt tggccgtaag
     1801 gatatggtct cctgtaatca taatgggctt aatgccagcc agcctacact gctgcacagc
     1861 taccttggct tcttctcttg gtgggtcaat cataccaaag agggccgtca gctctaggtc
     1921 tttttcaatt tcttctgaag taatattttc tggtaactca tctaagtatt tgattcctag
     1981 cgccaaaacc cttagggcct gggagctcat cttatcacta atggccctag cctggcttag
     2041 gtctgttttt atactaagac cttcaagcat atcaaaggca cccttggtaa tcacaagata
     2101 gcccttatct gttttatgga tggtggacat acgttttcgt tctgagtcaa aggggagctc
     2161 atagattcta ggaaactctt tttccagggc ttcttttctt tgtcccttga tatgggcagc
     2221 atagataagt gctgtttctg tgggatctcc caggtagagg atctccccct cttcttctct
     2281 tacacttcca ttactacaaa gggtggcata gcttagggcc tgggttaact cagaagagat
     2341 ttcaccctct atgctttctt ctttttcgcc tactgcatag ccttctagca gggtcatttg
     2401 attcatggta agggtaccgg tcttatcact acaaataacg gaggcacttc ccagagtttc
     2461 aacagcggga agttttctta taatggcatg tttttttacc atgcgctgga tgcctagaga
     2521 aagaacgatg gtaacaaccg ctggtaggcc ctcaggaatt actgatactg ccagggaaac
     2581 cgaggtcata aaaataacca tggggggcag gttgtcgata ataccaataa taaaaaccag
     2641 ggcacaagca gctagggcaa taaagccaag gtttttacct agtttaccca gtttttgctg
     2701 gaggggggtt tgtgtatctg cctcgctatc aagaagctgg gcaattttgc cgatttgggt
     2761 gttcattgcg gtatgggtaa ccagggctct tgctgttcca tagcttatag ggcagcccga
     2821 gtagaccata ttgaggcggt cgcctagagg aaccttgtcc tctatttcca ggctggcatc
     2881 tttttctacg ggaacagatt cccctgtaag ggcagattca tcacttttta aactactgct
     2941 ttcaataagg cgagcatcgg ccggaacaaa gtccccggct tctagatgaa tgatatctcc
     3001 tggaacaagc tgggagctat ccattacctg ctcatcgcca tctcgaaaga cccgagcctg
     3061 tagtgaggta agactcttta gagcctcaag ggccttttct gccttggcct cctggagggt
     3121 tcccattatg gcattcataa taacaataag aagaataagt aggggttcgg caaaagactt
     3181 actttgcccc tggtaaatgg caatgccaaa ggatactagg gcagcaata
//
LOCUS       DASRNA010000139         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1085845,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000139 DASRNA010000000
VERSION     DASRNA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085845"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..708
                     /locus_tag="VFD08_03840"
     CDS             34..708
                     /locus_tag="VFD08_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10183.1"
                     /translation="MTIIYDGKNLAGLCGKLSLDELAILSSRAQPQAKEAIYYMEKDQ
                     DPQMIKDFPPGWAVEMEAFDESLPLDYKDRIFTRDFKKPPLLGKICLWGSFDGVESLC
                     KYLASSSRISFGISGDSHRVQRLREISKNRADHFDGQPLHMVLGMGEISDTHLYLWDG
                     SLDELYRLREYYEELPRYHYMVQRGYLGKSFQEQAVIHGRYLGRISKKGWKWEWKKII
                     EIISSE"
     gene            765..1877
                     /locus_tag="VFD08_03845"
     CDS             765..1877
                     /locus_tag="VFD08_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HZK10184.1"
                     /translation="MKYLATTSFGMEGVLKNECVLQGFAIEKVEMGMVVFSGSLKDML
                     VANLTLRTAERIFWVLDESKPKSFDELYDFVTAYPYEDIMSEKAEIIVNAKSYRSTLY
                     SLRDIQTITKKAIVDRYRRKKGLRYMDETGQQYSYLVRIEKDHAYLLLDTSGQPLHKR
                     GYRQQHNQAPLRENFAAGLLLLSRYFGKGDFYDPLCGSGTIAIEAALIATDKAPGLER
                     NFQCERWNLLDRKESHMLRESLKERIKPAEFKILASDNDPRAISMARRNAQMAKVKIE
                     LKQEDLKDLEGLSTGGTLVTNMPYGERMGTSEEIELLEELMIDKALSLETWRLGILSG
                     NPNFAKQTGRRASKIRKFYNGQIDTWFYQYNPTKRW"
     gene            1877..>2830
                     /locus_tag="VFD08_03850"
     CDS             1877..>2830
                     /locus_tag="VFD08_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10185.1"
                     /translation="MKNRRWRLLIPAVLLLLLLFFGIRGLFPRDPVDLALDQEEEAGE
                     QKSLTLTPISELKMTKDIRDSFDDVFAEDDLVMYYEKGLLKAFDIDNQELWSRSFDSN
                     LILGKNVARTLVIEKSDGNVYHISRGGEILGSALGLGEIEQAHLTQDNRTLVFFRDNR
                     KILILDEFLQGSAEIGVESGAIINYSVSTKEEQVSLLTLEELEGELLSRLYIYSLEGR
                     LLQSRKEERLALNVFNAGRNTLIVYQDGLQFFDELLEEKGEFIPTNKVSFSKREGFHV
                     FLVTGSPNPLKEEGELEMLSFSLTNRKLEFSNKITDNYDNIY"
BASE COUNT          860 a          520 c          672 g          778 t
ORIGIN      
        1 ctataagtct agtatttaat ctaaggagga cttatgacaa ttatttatga tgggaaaaac
       61 ctggcgggtc tttgtggcaa actttcctta gatgagctgg ccattcttag ctccagggcc
      121 caaccccaag caaaagaggc catttactat atggaaaaag accaagaccc acagatgatt
      181 aaggactttc ccccgggctg ggctgtggag atggaggcct ttgatgagag tttgccccta
      241 gattataagg accggatttt tacaagggac tttaaaaagc ccccgcttct tggtaaaatc
      301 tgcctttggg gctcctttga tggagtggaa tccctctgta agtatcttgc tagtagcagc
      361 aggatttcct ttggcataag tggggactct caccgggtgc aaagactaag ggagataagc
      421 aaaaataggg ccgatcattt tgatggccag cctcttcata tggtcttagg aatgggggaa
      481 ataagtgata cccatctcta tttatgggat ggatccctgg atgaactcta ccgccttaga
      541 gaatattacg aggagctacc ccgctatcac tatatggtcc aaagagggta tttgggaaaa
      601 tcctttcaag aacaagcagt catccatggg cgctatttgg gacgtattag taaaaagggt
      661 tggaagtggg agtggaaaaa aataatagaa ataatttcat cagaatagat tttcccttct
      721 attcttagag ggaagctatg gtatactaaa gcagaggtaa aaaaatgaaa tatttagcaa
      781 ccacctcctt tggaatggaa ggtgttttaa aaaacgaatg tgtattacaa ggctttgcca
      841 ttgaaaaagt agagatggga atggtagttt ttagtggttc tttaaaggat atgctagttg
      901 ccaatctaac tcttcgcacg gccgaacgaa tcttttgggt tcttgatgaa tcaaagccta
      961 aaagttttga tgaactttat gactttgtaa cagcctatcc ctatgaggat attatgtctg
     1021 agaaggcaga aatcattgtt aatgccaaga gctatcgctc tacgctgtat agtctaaggg
     1081 atattcaaac aataacaaaa aaagcaattg tagaccgcta tcgcaggaaa aaaggcctgc
     1141 gctatatgga tgaaacaggc cagcagtatt cctatctggt gcggattgaa aaagatcatg
     1201 cctatttgct cctggatact tcaggccagc ccctgcataa aagaggctat cgccagcagc
     1261 acaatcaggc ccccttgcga gaaaactttg cagcagggct gctacttctg agccgttatt
     1321 ttggcaaggg agatttttat gatcccctat gtggctcagg gaccattgcc attgaagctg
     1381 cccttattgc cacggataag gctcctggtc tagaaagaaa tttccagtgt gaaagatgga
     1441 atcttttaga ccgaaaagaa agccatatgc tgcgcgagag tttaaaagag cgcatcaaac
     1501 cagcggaatt taagatccta gcctctgata atgatcctag agccattagc atggcccggc
     1561 gtaatgccca gatggctaag gttaaaatag aacttaagca agaagatcta aaagatctag
     1621 agggactttc aaccggaggc accctggtga caaatatgcc ctatggtgag cgtatgggta
     1681 cttcagaaga aattgaacta cttgaggagc ttatgattga taaggccttg agcctggaga
     1741 cttggagact gggtatttta tcaggaaacc caaattttgc caagcagaca gggcgcaggg
     1801 cgagtaagat tcgcaagttt tataatggtc aaatagatac atggttttat cagtacaacc
     1861 caactaagag atggtaatga aaaatcgacg atggcgtctg ctaattcctg ctgtccttct
     1921 tcttttgctc ttgtttttcg ggattagggg gctttttcct agggatcctg ttgatctcgc
     1981 cctagatcaa gaagaggagg ctggtgagca gaaatcgctt acacttacac caatttcaga
     2041 actcaagatg acaaaggata ttcgagacag ctttgatgat gtttttgctg aggatgatct
     2101 tgttatgtat tatgagaagg gactattaaa agcctttgat atagacaatc aagagctttg
     2161 gtctcggtct tttgattcca atcttatctt ggggaaaaat gtggctagga ccctggttat
     2221 agaaaaaagc gatggaaatg tatatcatat ttctagaggg ggagaaatcc taggaagtgc
     2281 cctgggtcta ggagagattg agcaggccca tcttactcaa gacaatcgca ccctggtgtt
     2341 ttttagggac aatcgaaaga ttctaattct tgatgagttc cttcaaggat cagctgagat
     2401 aggggttgaa agtggggcca ttataaacta tagtgtctca acaaaggaag agcaagtaag
     2461 tcttcttacc ctagaagaac ttgaaggaga gcttttaagt cggctttata tatactcttt
     2521 agaaggaaga ctccttcagt ctcgcaaaga agaaaggctg gccttaaatg tatttaatgc
     2581 aggtaggaac accctgattg tttatcaaga tggcctacag ttttttgatg aactcctaga
     2641 ggaaaaaggg gaatttattc ccacaaataa ggtgagcttc tcaaaaagag aaggttttca
     2701 tgtatttctt gttacgggtt cacccaatcc actaaaggaa gagggggagc tggagatgct
     2761 ttctttttcc ctaaccaata gaaagcttga attctctaac aaaattactg ataattatga
     2821 taatatatac
//
LOCUS       DASRNA010000140         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_269048,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000140 DASRNA010000000
VERSION     DASRNA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269048"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..2363)
                     /locus_tag="VFD08_03855"
                     /pseudo
     CDS             complement(62..2363)
                     /locus_tag="VFD08_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
BASE COUNT          697 a          536 c          400 g          887 t
ORIGIN      
        1 gtctaaaatt ccttgccaca tatagcccca gctattaatt tcttcttttg ctacatacta
       61 tttattctag aaaatctgta aaatcatcat tcaacctata acctagaaat agtgtggttt
      121 tctctccacc ttttcttggt ctctttctaa ctacctctcc aatggaagct agagaggatt
      181 taaaattcct agcattttct gtatagtagc cattgtccct gcaccagttt tggtaggcaa
      241 agtaaaggtt agatgttctt tcttcagcac cctcatcctt agttagtctt tcttctacaa
      301 aaagacctat tttgtcattt tccttatggt agtcataggt tgcctttttg accgaatcag
      361 gcacagtaaa gccttctttt tgtaattttc tatatccttc aagaatccag tttaggatgc
      421 cacttaggtt ttctccctta gaaaaaagat ttttaaggtt cttatcttgc tcccattctt
      481 caaagtgtct ttcaaaggga ataatcttta ccctgccgct ggttaagaga gttaggtctg
      541 tcaccatagg cagatggttg gtattaatga aaatcttaaa ttgtggccta aagtcaaagc
      601 tgttctcatg aaggaatcta gcattaatag tgtcattacc tgtaaggctt tttagtagag
      661 cagcacttag gattaacttc ttgtcaggtt cacttatatt tacaaatctt gcacctgcca
      721 gcctagctac atcttctgat ggtcctgaac ctgtggaatt catcttcatt cctatcgttt
      781 ctggcctgct ggtccttcca tagtcaccac agatatttaa gaaggtttcc atagaagtgc
      841 ccttgccatt tctggtagtt gctccaaaaa gaataaacat agactcgtag cgagtatcac
      901 cagtcaaggc atagcctagg cttttttgaa agaagtcagc cttttcctta tcaccacaca
      961 taacctcatc tataaatgct atccaacgat cacatttagc acttgggtca tagttcacac
     1021 cagccatctt agtaatgtgg tccagcttgg tgagggtaaa agtctccatt ttttaggttt
     1081 aaagttccat ttaagcagtt tagcaagtat atatccttat caaattcaga catggacagg
     1141 ggatagacat cttgagcatc tcttaaaata atttctctac ttcttctttc ttgccattta
     1201 cttgctgatt taatatagga ttttcttagg ttttcatcct ttatagaaag ggcataatac
     1261 ataatctgat tagctaggct tttacataaa ttcattacct ttaaatttcc ggtatcagaa
     1321 gcccagactc ttccgtcata aacaaaccac tttttccttt ctggtacata ccgagtaata
     1381 gacttatagt agtcagcaaa aaggttactg ttacctatat ctgttccgta gtatctagga
     1441 tttttaaaag gctcaagttt gtttagtttt tctaaattat ctttagtaaa atcttcatca
     1501 gctgtcagcc ttccaccact agggtcatag gtttcctttg tgttagctat ggccttctct
     1561 agggttattt gcccataggt tgagccagac tggaccctgt gccacttatc cctcattagc
     1621 ccacttgatt gaaagagcct gtccatttgt ttaatatctc taccacacca aaaagctaat
     1681 atagatgcta gagcaaggtc agcttcagat tgggaaggat aaacagaaat atctccctcc
     1741 catagagctt taaacttctc tcctgttgct gatagactag ctttttctat aactgaagca
     1801 tcagatagga aggatttgct ttcactttca tttacagctt tgtttacctc tggcctttgc
     1861 atatacctat ccagtaaggt tttaagctga tcattagcta gtaagatctg gcctttgtta
     1921 ataacttggc ctgtaatagt cacatatttg ttagtagcac catctacata gacctctaag
     1981 cctagctttt gattattgat ataataaagt tttttgtcat agaaaaattt atctacaaga
     2041 aatataattc ttattccttt ccctgaaggg cttttttctg tataagagtc cttaaaaatc
     2101 tccattacgt cttttgctaa atcagagtag ccaccttttt catcaataca attatctata
     2161 tcaatggcag atattccatt aaagatgcct atgcctaaac catcaaagct atctaaaact
     2221 gaaagagcac tcttaaagtc ggtaaaggta gaaatcttat tagcccttgc tcgttttcca
     2281 tcgacttgat aggggacttt tcccttgcca gcttcattct tccacaggca aaacctagcc
     2341 ttgtctttta aatagtctgg tatcttttca aatttcttta tagtctttca cctcctctaa
     2401 tttattagga cccaaaacac tagccttcct aaggtcttgc cttgtttgac atagccttgt
     2461 ataaccttta aagacacgct cggataatcg tcatggcata ttcatataaa agtattcttc
//
LOCUS       DASRNA010000141         9839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_350897,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000141 DASRNA010000000
VERSION     DASRNA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350897"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFD08_03860"
     CDS             <1..1010
                     /locus_tag="VFD08_03860"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZK10186.1"
                     /translation="RSWIDWQDKYRNRQAQALEKIREQEKINFQFWIFTQFIFHKNMK
                     DFSDYAKSKGIDIIGDIPFYVAEDSVEVWSRPELFLLGEEGFVAGTPPDYFSDDGQLW
                     GNPIYNWELMEKENFSWWVDRLRAQCSFYRYLRLDHFNAFARYWSIPKGKSAKKGYMK
                     EGYGLEILEQVPDLIENQSLIAEDLGTETKAAEVVRKAFCIPGMRILQVGFSPGEDNP
                     HLPYSYEKDLFVYTGTHDNDPIMSWYAKAPLKVREEVKAYLNTQEPRALDFVRLLMAS
                     AADNVVIPVQDLLGLGSQARMNLPGTLDNNWIWRLESLDPLAPFEDILRHYSKTYRRN
                     F"
     gene            1031..3373
                     /locus_tag="VFD08_03865"
     CDS             1031..3373
                     /locus_tag="VFD08_03865"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZK10187.1"
                     /translation="MIFDLKNTLRSDYAVSLERAKDYEIYNALVKVVMRLIAPSWESD
                     VEGRFGRNAYYLSSEYLMGRLLSNNLTNLGIDKIINESLEEIGLDYNELEKIESDTGL
                     GSGGLGRLAACFLDSAATHNYPLHGYGIRYEYGIFKQEFKDDQQIETADTWLRYGDPW
                     SIRRESERKVIQFSNQKVYAVPYDSPIIGYDAETINTLRLWRSEPIDEFNFNLFNNQD
                     YDLALQEKNRAEDISRVLYPNDTRREGKILRLKQQYFFTSASIQDLVEKHLRKHSSLE
                     NFHMYNALQLNDTHPAVAVAELLRIFSDEHGLDMAAALEIAKKTFNYTNHTIMSEALE
                     KWDENLYREILPRIHEIIGWIEGKRREKLAAKKIPQNLIEKTSIFSQGMIHMANLAVF
                     GSTQVNGVAQLHSSIIKKSVLNDWYRIYPEKFTNVTNGITQRRWLLLSNPELAQLLTK
                     TLGSSEWIKELSLLTSLGGMEYDPSFLQEFKTVKQIKKNQLAATIYEKEGIYIDPNSL
                     FDIQIKRLHEYKRQLLNAFHILDLYRGLKEGRITDFTPRTFIFGAKSAPGYRRAKGII
                     SYILQLAKMINNDPAMDGLLKVVFVTNYNVSYAEKLFPAADISEQISTAGKEASGTGN
                     MKFMLNGAITLGTMDGANVEIVEEAGIENNIIFGLRSEEIQEMKEYNPRYYYENTPGL
                     KTLVDSLIDGTFEDKDGVFREIYNSLLHENGWDHPDEYFVLADYESYREAQKRAGELY
                     KDQDLFARMCIRNMSSAGKFSSDRSVEDYVNNIWHIEKLD"
     gene            3382..4308
                     /locus_tag="VFD08_03870"
     CDS             3382..4308
                     /locus_tag="VFD08_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZK10188.1"
                     /translation="MYTEYKLDKAYEEAKHIPIDDSSRIVIFSDAHRGDGSFTDEFAP
                     NENIFLHALQYYYDEGFSLIENGDSEDLWLNNDFKYIYYAHPDVYELIRKFHKQNRYT
                     MIFGNHNIIMGKESYCQNHFWNVYDFNLGEEVPFLDGLVPVEAVRLGYRDFKEDVFIL
                     HGHQGDLINDEFWIGSRFLLRKLWRHLRSIGFRSPVSPARSEQKRHRVEKVYSKWIDE
                     NNQMTIIGHTHRPKFSHPGEIQYFNTGSATRPRRVQTIEILGGSIFLVEWRIRPTPLG
                     VLQVKRRVVDGPKALSKYLKREPFKEEKNRIK"
     gene            complement(4326..5255)
                     /locus_tag="VFD08_03875"
     CDS             complement(4326..5255)
                     /locus_tag="VFD08_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10189.1"
                     /translation="MKEINKLDLKEAVLLLDAYLNNKPFPKEVEGFDKDKLWEKISSL
                     VQEEGEGTLFPRQLEKALEIYKNSPDEFRDILLEAKKIPPLRYSSRKGDWEILPESLK
                     DSILYAYLFEGLSLRELDEAYLLLDSEKTKGFESLRILHYMGVTGDFRGIFAELELNE
                     GIRCLKKEEKVYGYLIRALQRYRYSLYQEEAHSENDNLQSLVREGSGFLYYTTRYERS
                     PYLRKQAIDFHGTSCMACGFDFEDFYGELGKDFIEVHHRVGFPDPRQDLFVDPELDLL
                     CLCSNCHRMIHRKRGAILEIEELRKIIAEEKSS"
     gene            complement(5378..6709)
                     /locus_tag="VFD08_03880"
     CDS             complement(5378..6709)
                     /locus_tag="VFD08_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZK10190.1"
                     /translation="MDKELLKKMACEAIDQEAKKIHDFTLDVYHNPELGFKEFETTKK
                     TADFLREIGLEVEENIAITGCRANTGAKEGQPNLCIMGELDSVVCADHKDAGKDGASH
                     SCGHHVQLGAMLGSAIGLVASGVMEHLDGSVEFMAVPAEEFIEIDYRSKLIAEGKIKY
                     AGGKQELIARGYFDKIDISMMMHTSNHTPPLKAVLGTSSNGFIGKKANFIGREAHSGG
                     APHLGINALNAASLALNNINAQRETFKDEDHIRVHPIITKGGDIVNVVPSDVRLETYV
                     RGSTIEGMQAANKKVNRSLKAGAMAVGGKVEIQEIPGYLPLKPDQKVDSLFKENLLAF
                     IKEEEIKMSGTMTGSTDFGDITHIMPGIHPMIGGVSGAAHTREFSVDDVNLAYIIPAK
                     AFAMTAIDLLYDGAKGAKEISKNFKPSMTRQEYLAFLEESSSKKTFSFEEE"
     gene            complement(7159..7971)
                     /locus_tag="VFD08_03885"
     CDS             complement(7159..7971)
                     /locus_tag="VFD08_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HZK10191.1"
                     /translation="MSAISMQKPILPKWKPYNYEIIPVHVMYAEPLSKGIFTYIINYD
                     QTMALHGGCFLIKGVEGFNILVDTGPTVEDFHNHGFPGTSIKAMPQALIETTGLRPED
                     IDVVILTQLHHDHCPCAHMYTKAKFIVQKKEWESCHNPPACYRALYNPEYVKDINPSF
                     VEGDVIELFPGISLLYTPGHTLGSQSISIDTKEGRVIICGLCCNEDNFNPPEELRKVY
                     PEVLVPGLHVDCEVAYNSMLRVKREADYIITLHDKKSFERGVCPSPLWPRYK"
     gene            8295..8819
                     /locus_tag="VFD08_03890"
     CDS             8295..8819
                     /locus_tag="VFD08_03890"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HZK10192.1"
                     /translation="MLKKFTELPDAEKKDLIDKAASLGYEYERKYGNCPQCTIAAIQD
                     TFGIIDDDVFKQSYGFGAGIGLTSKGTCGALSAAILIIGSLHGREKADFHSGRNAKCY
                     ELSKQLVEKVEEEYGGILCHEIQRKIMGDSFDLNKQEEFLAFEAAGGHDDKCPSVIAF
                     VAGYLAELIVEGEL"
     gene            complement(8895..9542)
                     /gene="fsa"
                     /locus_tag="VFD08_03895"
     CDS             complement(8895..9542)
                     /gene="fsa"
                     /locus_tag="VFD08_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HZK10193.1"
                     /translation="MKLFIDTANVEEITEVNDWGVICGVTSNPSLIAKEGRDLTEVIK
                     EIAEIVDGPISVEVISLEWQGMVDEAREFAKIHKNIIVKIPMTRDGLKAVKILAKEEI
                     KTNVTLVFSANQGLLAAKAGASYVSPFVGRLDDIGNEGMDVVKDIAEIFSIYKMDCQI
                     ISASIRHPMHVLQSAKAGAHIATIPYKVFSEMLGHPLTDIGIERFLEDWKDFETK"
BASE COUNT         2906 a         2064 c         1864 g         3005 t
ORIGIN      
        1 gtcgttcctg gatagactgg caggataaat atcgaaatag acaggcccaa gccctggaaa
       61 aaatcagaga gcaagaaaaa atcaactttc aattttggat atttacgcaa tttatctttc
      121 ataagaatat gaaagatttt agtgactatg ccaaaagcaa aggaattgat attataggag
      181 atatcccttt ttatgtagca gaagattctg ttgaggtctg gtctcgtcct gaactctttc
      241 ttttaggaga agagggcttt gtagccggaa cacctcctga ttatttttcc gatgatggcc
      301 agctctgggg aaaccccatc tacaattggg agctaatgga aaaagaaaac ttttcttggt
      361 gggtcgatcg ccttcgcgcc cagtgctcct tttatcgata ccttcgccta gatcacttca
      421 atgcctttgc ccgctactgg agcattccca aaggaaaatc agcaaaaaaa ggctatatga
      481 aggagggcta tggcttagaa atattagaac aagtacctga tctaatagaa aatcaaagct
      541 taattgccga agatttgggt acagaaacaa aggcagcaga agtggtaaga aaagcctttt
      601 gcattccagg aatgcggatt cttcaggttg gcttttcccc aggtgaggac aatccccacc
      661 ttccctatag ctacgagaaa gatctctttg tctatacggg aacccatgac aatgacccta
      721 ttatgtcttg gtatgctaag gcccccttga aggtgagaga agaggtaaaa gcctatttaa
      781 atacccagga gcctagggca cttgactttg tgcggcttct aatggccagt gctgctgata
      841 atgttgttat acctgttcaa gatttgctgg ggcttggaag ccaggcccga atgaatcttc
      901 ccggaacatt agataataat tggatttggc gccttgagag cttagatcca ctagctccct
      961 ttgaagatat tcttcgccac tactcaaaga cttataggag gaatttttga atttactcag
     1021 taaagataca atgatatttg atttaaagaa cacactaaga agcgactatg cagtctctct
     1081 agagcgcgcc aaggattacg aaatatacaa tgccctggtt aaggttgtta tgcgccttat
     1141 tgctccctca tgggaaagcg acgtagaagg acgctttgga agaaatgcct attatttgtc
     1201 cagcgaatat cttatgggca ggcttcttag caacaatcta acaaatcttg gcattgataa
     1261 aataatcaat gaaagcctag aagagattgg ccttgactat aatgagttag aaaaaattga
     1321 aagcgatact ggcctaggaa gtggcgggct tggccgtctg gctgcttgtt ttttggactc
     1381 agctgccaca cacaactacc ccctacacgg ctacggtatc cgctacgaat atggcatatt
     1441 taagcaagaa tttaaggatg atcagcaaat tgaaacagcc gacacctggc tgcgctatgg
     1501 agatccatgg tctattcgac gcgaaagcga aagaaaagtg attcagtttt ccaatcaaaa
     1561 agtatatgct gttccctacg actcgcccat tattggttat gatgcagaga ccatcaatac
     1621 gctccgccta tggcgaagcg aacccattga tgagtttaac tttaacctct ttaataacca
     1681 ggactatgat ctggccctgc aagaaaaaaa tagagccgaa gatatatctc gggttttata
     1741 tcccaatgac acgcgaagag aaggaaaaat cctacgacta aaacagcagt acttcttcac
     1801 atcggcttct atccaagacc tagttgaaaa acatctccgc aagcatagta gcctagaaaa
     1861 cttccatatg tataatgccc ttcagctaaa tgacacccat cctgctgtgg cagtggccga
     1921 acttcttcgc atcttttctg atgagcatgg cctagatatg gcagcagctc tagaaattgc
     1981 aaagaaaacc tttaattaca ccaatcacac cattatgagt gaggcgctgg aaaaatggga
     2041 tgaaaatctt taccgggaaa tcctacctag aatccatgag attattggtt ggattgaagg
     2101 aaaaagaaga gaaaaacttg ccgcaaaaaa aatcccccaa aatcttattg aaaagacatc
     2161 catcttttcc cagggtatga tccatatggc aaatcttgcc gtttttggct ccacccaggt
     2221 aaatggggtg gcacagctcc actcatccat tattaaaaaa agtgtcctaa atgactggta
     2281 ccgtatttat ccagaaaagt tcaccaatgt taccaatggc atcacccaaa gacgctggct
     2341 tcttctttct aatccagaac ttgcccagct tcttacaaaa acccttggaa gttctgagtg
     2401 gattaaagag ctcagccttt taacatctct tggtggtatg gagtatgacc cttctttcct
     2461 ccaggaattt aagaccgtca aacaaataaa gaaaaaccag ctcgcagcca ctatttatga
     2521 aaaagaagga atctatattg atccaaacag cctctttgat atccagataa aacgcctgca
     2581 tgaatataaa agacaacttc ttaatgcctt tcatatccta gacctctacc ggggccttaa
     2641 agaagggcgc attacagact ttacccccag aacttttatt tttggagcaa aatctgcccc
     2701 aggataccgc agagccaagg ggattatctc ctatatccta cagctagcta aaatgatcaa
     2761 caatgatcct gcaatggatg gcctgctaaa ggtagtcttt gtaacaaact acaatgtatc
     2821 ctatgctgag aaactctttc ctgcagcaga tattagtgaa caaatatcaa cagcaggaaa
     2881 agaagcctcg ggaacaggaa atatgaaatt tatgcttaat ggagccatta ccctaggcac
     2941 catggatgga gccaatgtgg aaatcgtaga agaggccggg attgaaaata atattatttt
     3001 tggacttcgc agcgaagaaa tccaagaaat gaaagaatat aatccaagat actactatga
     3061 aaacactcct ggattaaaaa cccttgtcga ctctcttatt gatggcacct ttgaagataa
     3121 ggatggagtc tttagagaga tatacaactc ccttcttcat gaaaatggat gggatcatcc
     3181 cgatgagtat tttgtactgg ctgattatga aagttaccga gaagcccaaa agcgtgcggg
     3241 agaactttac aaagaccaag acctctttgc taggatgtgc attcgaaata tgagctctgc
     3301 cgggaaattt tcttctgatc gatccgtaga agattatgta aataatatct ggcatattga
     3361 gaagctagac tagaggaaaa aatgtataca gagtataagc ttgataaagc ctatgaggaa
     3421 gcaaaacaca ttcccattga tgacagttcc aggattgtca tttttagcga tgcccatagg
     3481 ggggacggaa gctttacaga tgaattcgcc cccaatgaga atatttttct tcatgctctg
     3541 caatattact acgatgaggg ctttagcctt attgaaaatg gtgatagcga agacctctgg
     3601 ctcaacaatg actttaaata catctactat gcccatccag atgtctatga gctcattcgc
     3661 aaattccaca aacaaaaccg ctataccatg atttttggaa accacaatat tattatgggt
     3721 aaggaatctt actgccaaaa tcacttttgg aatgtctatg acttcaacct tggagaagaa
     3781 gttccctttc ttgatggttt ggtgcccgta gaggccgtca gattaggcta tagggacttt
     3841 aaagaagatg tatttattct ccatggacat cagggagatt taatcaatga tgagttttgg
     3901 attggctcac gcttcttgct aagaaaactt tggcgacatt taagaagtat tggctttcgg
     3961 tctcctgttt ctccggctag aagcgaacaa aagcgccata gggtggaaaa ggtttattcc
     4021 aagtggattg atgaaaataa tcaaatgacc attattggcc atacccatag accaaaattt
     4081 tcccatcctg gtgaaattca atatttcaac actggcagtg ctacaaggcc aaggcgggtg
     4141 caaacaatag agattctagg tgggtctatt ttcttagtag aatggcggat tcgccctacc
     4201 cctctggggg ttctacaggt aaaaagaaga gtggttgatg gccccaaggc cttaagcaaa
     4261 tatttaaaaa gagagccctt taaagaagaa aaaaaccgga taaaatagtc cggttttttc
     4321 tttggctagc tacttttttc ctctgcaatt atctttctta gttcttctat ttctaaaatc
     4381 gccccccgct ttctatggat catacggtga caattcgaac ataggcataa taaatccagc
     4441 tctggatcaa caaaaagatc ctgccttgga tcaggaaagc caaccctatg gtgaacttcg
     4501 ataaaatctt tacccagctc cccataaaaa tcttcaaaat caaagccaca agccatacag
     4561 gaggtcccat gaaagtcaat ggcctgcttt cttaaataag gagacctttc atagcgggtt
     4621 gtatagtata aaaatccgct tccttctctg actagggact gtaaattatc attttcagaa
     4681 tgggcttctt cttggtaaag ggagtagcga tagcgctgca gggcgcggat tagatagcca
     4741 tacactttct cttctttttt aaggcacctt attccttcgt ttaattctag ctcggcaaaa
     4801 ataccccgaa aatctcccgt tacccccata tagtggagga ttctaagaga ctcaaaacct
     4861 tttgttttct cagaatcaag caaaagatat gcttcatcaa gttctcttaa actaaggccc
     4921 tcaaaaagat aggcatacaa aatagagtcc tttaggcttt ctggtagaat ttcccagtct
     4981 ccttttctac ttgaataacg caagggtggg atttttttgg cctctagtag aatatctcta
     5041 aattcatctg gactattctt ataaatttcc agggcttttt ctaactgcct gggaaaaaga
     5101 gtcccctccc cctcttcttg aactagggaa gagattttct cccataactt atccttatca
     5161 aaaccctcta cttctttggg aaagggttta ttgtttaagt atgcatcaag aagcaaaaca
     5221 gcttccttta ggtctaattt gtttatttct ttcatctcct ccccctttga tttctatttt
     5281 atcatattta tgtttttttt gataactatt agatgtcaaa aaaggagcgg gtaagcgctc
     5341 ctttttcagg ggtcatataa atctcttctt ctagttttta ctcttcttca aaactaaagg
     5401 tctttttact agaagattct tcaagaaaag ccaaatattc ctgcctggtc atagagggct
     5461 taaagttctt actaatctcc tttgccccct ttgccccatc atatagtaaa tcaatggctg
     5521 tcatagcaaa ggcctttgct ggaattatat aggcaagatt tacatcatcc accgagaatt
     5581 ctcttgtatg tgctgctcca gaaacgccac caatcatggg gtgaatcccc ggcataatat
     5641 gagtgatatc tccaaaatca gtagagcctg tcattgtacc gctcattttt atttcttctt
     5701 ctttgataaa agctagcagg ttttctttaa aaagactatc caccttctga tcaggtttta
     5761 agggtagata gcctgggatt tcttgaatct ccaccttgcc ccctacagcc atagcccctg
     5821 cttttaagct tcgattgacc tttttattgg ctgcttgcat gccttcaatg gtgctgcctc
     5881 taacataggt ctccagcctt acatctgatg gaacaacatt tacaatgtct ccccccttgg
     5941 taataatggg atgtaccctt atatggtctt catctttaaa ggtttctctt tgggcattga
     6001 tgttattaag ggctaaacta gcagcattta gggcattgat tcccaaatga ggggctcccc
     6061 ctgagtgggc ttcccttcca ataaagtttg cttttttgcc tataaagcca ttgctgcttg
     6121 ttcctagaac agctttaagt ggaggagtat ggtttgatgt atgcatcatc attgaaatat
     6181 caatcttatc aaaataccct cttgctatca gttcttgttt tcctccagcg tatttgatct
     6241 ttccctctgc aatgagcttt gagcgataat ctatttcaat aaactcctcg gctggcactg
     6301 ccataaattc aaccgaccca tctaaatgct ccataactcc tgaagcaacc aggccaatag
     6361 cagaacccag catggctcca agctggacat gatggccaca ggaatgggag gccccatctt
     6421 ttcctgcatc tttatgatct gcacaaacaa cagaatctag ttctcccata atacaaagat
     6481 tgggctggcc ttcttttgcc cctgtattgg cacgacaacc cgtaatggca atgttttctt
     6541 ccacttccag accaatttct cgtaaaaaat cagctgtttt ttttgttgtt tcaaactctt
     6601 taaaacctag ttcggggtta tgataaacat caagggtaaa gtcatgtatc ttctttgctt
     6661 cttggtctat ggcctcacag gccatctttt taagcagctc tttatccatt ttcatctcct
     6721 cttttgcgta agttctctta tatttattcc agtagtagat tcatacccag ggagaaatta
     6781 taactctaca ttgtaaaaaa gaccatgtaa attgtttaag ctgatcttcc tcccgtgctt
     6841 ttttatttaa gaaaagtctg acagcttaag cctcattttt cttaggaact gattaagtgt
     6901 tttttcttgt tttccctcca agcaagaaag ggttgaataa taaaaacaag aaccagggct
     6961 tttttaggct gcccatagta cttgtttttt acctgttatt attttcaaat ggcattctta
     7021 ttctaaatcc agtggctcaa taactagccc agcctccata ccaaaccttt attaacatca
     7081 caagattgtc ctctaaggta atctcctcta ataataacta ttcttccagg cttagctgtc
     7141 tatctatcta ctttttcatt atttgtacct tggccaaagg ggactgggac aaactcctcg
     7201 ctcaaagctt tttttatcat gaagagtaat aatataatcc gcttcccttt ttactcttag
     7261 catactattg taggcgacct cacaatcaac atgtaggcca gggactaaca cctcaggata
     7321 gacttttctt agttcttctg gaggattaaa attatcttca ttacagcaaa gtccgcaaat
     7381 aatcaccctg ccctcttttg tgtcaataga aatggattgt gaaccaaggg tgtggcctgg
     7441 tgtataaagt agtgagatgc caggaaaaag ctcaatcaca tcaccctcaa caaagcttgg
     7501 attgatatcc tttacatact ctggattata tagagctcta tagcaggcgg ggggattgtg
     7561 gcaagactcc cattcttttt tttgaacaat gaattttgcc ttggtgtaca tatgggcaca
     7621 aggacaatgg tcatgatgga gctgggttaa tattacaaca tcaatatctt ctggccttaa
     7681 tcccgttgtc tctattagtg cctggggcat tgctttgatg ctagtgccag gaaatccatg
     7741 gttgtgaaaa tcttctaccg ttggaccagt atccactagg atattaaatc cctccactcc
     7801 tttgattaag aaacagccac cgtgcagggc cattgtttga tcataattga tgatataggt
     7861 aaatattccc tttgaaagag gttccgcata cataacatgt acgggaataa tttcatagtt
     7921 atagggcttc catttgggca ggattggctt ttgcatacta attgcactca ttatttcact
     7981 cccttttttc tgtcctagag tttttgtgga ttttgctagt gccgcccttt aaagtcactt
     8041 gtaaacacat ttgtaaggat agtaaaattt gttcacatat attatactgt tttaataaag
     8101 agatagtcaa atctaatttt ttctagaaaa aaacaagaag acaagctcct tcttacaaag
     8161 tccaaataca tagtgttata gtataagggg tagtggctat ataaagccac agatcttgga
     8221 aagtcaccgg ctgtagcttt gacccctgcc ctatttgata agctaaatat attttacaag
     8281 gagagtatca tattatgcta aaaaaattta ccgaattacc tgatgcagaa aaaaaagatc
     8341 taatagacaa ggcagcttca ctaggatatg aatacgaaag aaagtatggt aactgtcctc
     8401 agtgcactat tgcagcaatc caagatacat ttggaattat tgatgatgat gtttttaaac
     8461 agtcctatgg ttttggcgct ggcataggcc ttacttctaa ggggacctgt ggcgctcttt
     8521 ctgccgcaat ccttattatt ggttctttac acggtcgaga aaaagctgat tttcatagtg
     8581 gcagaaatgc aaagtgttat gagctaagta aacaattagt agaaaaagta gaagaagaat
     8641 acggcggaat cctttgccat gagattcaaa gaaaaataat gggagactct tttgacttaa
     8701 ataagcaaga ggaatttctt gcctttgaag cagctggagg ccatgatgat aaatgtcctt
     8761 ctgttatcgc ttttgttgct ggctatcttg ctgagctgat tgttgaggga gaactctaag
     8821 tctcaaaaag tctatgaatg taaaaaaaga ccacttacta ttataatggt aagaggtctt
     8881 tttgctttta tttattattt agtctcaaag tctttccagt cttctaagaa tctttctatt
     8941 ccaatatccg taagaggatg acctagcatt tcactaaata ccttataggg tatagtcgca
     9001 atatgggccc ctgctttagc tgactgtaat acatgcatgg gatgccttat gctggcggaa
     9061 ataatttggc agtccatctt gtaaatagaa aatatttctg ctatatcctt aaccacatcc
     9121 atcccttcat ttccaatatc atcgagtcta cctacaaagg gactgacata acttgcaccc
     9181 gcttttgctg ctaagagtcc ttggtttgca gaaaagacta aggtcacatt ggttttaatt
     9241 tcttcttttg ctaatatttt tactgccttt agaccatctc gggtcatggg gattttaaca
     9301 attatatttt tatgtatttt agcaaattcc cgggcctcat ctaccatgcc ctgccactca
     9361 agagaaatta cttctacact aattggccca tctactatct ctgctatttc cttgattact
     9421 tcagttagat ctcttccttc tttggcaatc aaacttggat tactggttac tccacagatg
     9481 actccccaat cattcacttc tgtaatttcc tcaacattag cagtgtcaat aaacaatttc
     9541 acataatcct ccttgttttt ccttttattt gcatattttt cttactcatt tttttacaaa
     9601 agatttgtgc ttaaagcttc taatatcctt tattaggtgc tttagatcag taggttagta
     9661 tttaggcggc aagcttcctt tctcttgcat aaagcgctat aagaggcctt aaaagacaag
     9721 cttgcccctt aagactggcc ctatctgcta aaactaggga tagaccccta tctttcctct
     9781 atttagcttt agctaaggta atctattgtc ctaattttag gtaattacaa gcatattat
//
LOCUS       DASRNA010000142         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_351474,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000142 DASRNA010000000
VERSION     DASRNA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351474"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1601
                     /gene="murC"
                     /locus_tag="VFD08_03900"
     CDS             204..1601
                     /gene="murC"
                     /locus_tag="VFD08_03900"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZK10194.1"
                     /translation="MKDLFSPNTNKIHLIGIGGSSMVAVAEILLDKGYILSGSDMAKN
                     SNVKRLESKGIPITIGHFAQSVAKADLVIYTSAVHDDNVEIIEAKKRGIPLIKRSHFF
                     QKLTEKYSHSVAISGAHGKSTVTAMVSVMMAESPMEPTLVVGAGVHELDGNVRLGKGD
                     LIVNEACEFEASFLDFERTISVILNIDDDHLDYYGSIDHIQDAFVEFANGTKENGFVL
                     MNADDPLSMEILPRIRKKVVTFGLDNEADYRAKNHHYDHQGYLSFDIYHKKDLLGSVE
                     LSVPGIHNIQNALAATAIVHHYHGDVKKELQTLQKFKGAIRRFDPHGDLNGALLYDDF
                     AHHPKEISKTLEAARSVFPDKRIVVGFQPHTYSRTKLLFNDFLNSFKDADVVWIMDIF
                     AAREANDPSIHSKMLVDKMTNPPASYVPTFEDLRDKMRLELKEGDVFFSVGCGDVYRI
                     FDEFLFVDEKEAQSC"
     gene            1595..1747
                     /locus_tag="VFD08_03905"
     CDS             1595..1747
                     /locus_tag="VFD08_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10195.1"
                     /translation="MLKKFFASYLVGLILFGALFFGLSSKLSPKNGFRPMPIEAEGLS
                     IEEIMP"
BASE COUNT          805 a          492 c          476 g          771 t
ORIGIN      
        1 aaataattca aatctggcta ttggctaaag cttatttatc actagccact tactatcctc
       61 ttcatattat cattaaaaag gaaaaaaatc accctagctt ataaaaacga ctctcctact
      121 tattaaataa ttatggctaa aactttattg gtagagattt ttatccatgc tataatggat
      181 aggacaaatt gaaaggaaca tttatgaaag atttattctc acctaataca aacaaaattc
      241 accttattgg tattggtggc agtagcatgg ttgctgttgc cgaaatactt ctagataaag
      301 gctatatttt gtccggatca gatatggcca aaaatagcaa tgtaaaaaga ttagaatcaa
      361 aagggattcc tattaccata ggacattttg ctcaaagtgt agcaaaggct gatcttgtta
      421 tatatacttc ggctgtccat gatgataatg ttgaaatcat tgaagcgaaa aaacgtggca
      481 ttccccttat aaagcgttcg catttcttcc aaaaactaac agagaaatac tctcattctg
      541 ttgccataag tggagcccat ggcaaaagta ccgttactgc tatggtatcg gttatgatgg
      601 ctgagtcgcc catggaaccc acactagtag ttggcgcagg agttcatgaa cttgacggaa
      661 atgtccgcct gggcaagggt gatcttattg ttaatgaagc ctgtgaattt gaagcaagct
      721 ttcttgattt tgaaagaaca atctccgtta tactaaatat agatgacgac catttggatt
      781 attatggctc cattgatcat atccaagatg cttttgttga atttgccaat ggtaccaaag
      841 aaaatggttt tgtgcttatg aatgcggatg atcccctaag tatggaaatc ctgccccgta
      901 taaggaaaaa ggtagtcacc tttgggcttg ataatgaagc tgattatagg gctaaaaacc
      961 accactatga tcatcagggc tatctaagct ttgatattta ccataaaaaa gacttgcttg
     1021 gttctgttga actctcagtc cctggcattc acaatattca aaatgccctg gcggccacag
     1081 ccatagttca ccattatcat ggtgatgtaa aaaaagagct ccagacgctc caaaagttca
     1141 aaggagccat caggcgtttt gatccccatg gggatcttaa tggtgcccta ctctatgatg
     1201 attttgctca tcatccaaag gagatctcca aaactcttga agctgcgcgc agtgttttcc
     1261 ctgataaaag aattgttgtt ggttttcaac cccataccta ttctagaaca aagcttcttt
     1321 ttaatgactt tttaaactct tttaaggatg ccgatgtggt ttggattatg gatatttttg
     1381 ctgctagaga ggccaatgat ccttctattc acagcaaaat gttggtggat aaaatgacaa
     1441 atcctcccgc aagctatgtc ccgacatttg aggatttaag agacaaaatg cgcttagagc
     1501 taaaagaagg agatgtcttc tttagtgtgg gctgcggaga tgtctaccga atttttgacg
     1561 aatttctttt cgtcgatgaa aaggaggctc aatcatgcta aaaaagtttt ttgctagtta
     1621 tcttgtaggg ctcatacttt ttggggcact atttttcgga ctttcctcaa aactatcccc
     1681 aaaaaatggt ttccgcccca tgcccatcga ggcagaaggt ctgagtatag aagaaattat
     1741 gccataaaga cggcttctgg taataatatg acccccaaaa gttggaccta gaatccaaca
     1801 aagggggtca tattaataat agggttattt ctttgttttg tttatctaag taccctataa
     1861 agctagaaaa ggcccatgag tgttgcgact gtagcataat cttgccctct atttatttct
     1921 atcgactatt tttctaccat catctgatac ataaccaata tttttaaaga aagcattctc
     1981 aagtttacga gtcgcttctt gaagaaaaag ctttttacat ctatcataat tgtttcagtc
     2041 tcagtatccg aagcaagtat tctttttttg atttgtattt ctatttcagc ttcttcctca
     2101 actgttttct tcgaaccaca acccctaatc atcaagataa aattatgatt tacttaaaag
     2161 gaaaaatcta agaaactaat tctggccatc aatatcgccc ttcctatctc tggtctgctt
     2221 cagctcacct ggggaaatgt gctcaaaaat aagaaacagt ttaaagattt tttatagggg
     2281 gaggagaaac aaagaaaaag tgaaaaatca agttcaataa gaaaagctcc ttaaagaatt
     2341 tatgacacca tgagcgcgaa gggtttctct tgcctactag agaaaagccc ttccttatct
     2401 agccaaggca gtagctatca aagataatat aaggaaccca caccctatga agaaacaagg
     2461 gttaccacgc caggagagac ggatttagcc ccgctcaaac catggatacc ttaaagcaca
     2521 taagtttgca gttaccaaaa gata
//
LOCUS       DASRNA010000143         4915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_351493,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000143 DASRNA010000000
VERSION     DASRNA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351493"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..322)
                     /locus_tag="VFD08_03910"
     CDS             complement(29..322)
                     /locus_tag="VFD08_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10196.1"
                     /translation="METQIVFSLKELLLASVLIALVVLIVYIILVLREVLLSVKLLRT
                     VVDEKREHIDEILEITPSIMGNVDRISDIAAKGTESAYKGAVGLVEKIKQGRG"
     gene            complement(335..1693)
                     /gene="lpdA"
                     /locus_tag="VFD08_03915"
     CDS             complement(335..1693)
                     /gene="lpdA"
                     /locus_tag="VFD08_03915"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZK10197.1"
                     /translation="MKDLIIIGGGPSGYAAALRAKDRGLDVLLIEEDRLGGTCLNRGC
                     IPTKSLVKNAEVFRTAKNLKAYGITIDGYTFDYAKVKQRKDKITDQLISGIELMFKKK
                     EIPVIKGRGAFVDSTTIEVNNQLYRAKNFLVATGSVAFNPPIPGSDLPGVLGSDQLLE
                     LEELPEHLFVSGASIVGLEFASIFLEFGSKVTVSGRTFLKREDLEIQKRAQNYLRRQG
                     MKFYLGGRMQSIEKTPQGLKVTVETKKGPKEVFCDKVLLSAGRKPNFSCNLEAAGINY
                     KPQGILVDDELRTSQPHIYAAGDVIGGDMLAHLASYEALNIVERLSGNPQKLDYRAVP
                     SCTFVYPEIASVGKNEEDLKKEKVDYKTSKFLYRASGKALAMDEPEGFVKMIADKSGR
                     ILGVHILGVGASDLISEATLAIKHNLSVKELYDTIHAHPTLPELLMEAAMAIDGLAIH
                     QL"
     gene            1757..2029
                     /locus_tag="VFD08_03920"
     CDS             1757..2029
                     /locus_tag="VFD08_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZK10198.1"
                     /translation="MNYIYILRCGDESLYTGWTNNLEKRIDTHRKGKGAKYTRSRLPV
                     SLVYFESFDRPGEARKREAAIKKMSRKEKLALIEGKEKKTPKGLND"
     gene            complement(2019..2369)
                     /locus_tag="VFD08_03925"
     CDS             complement(2019..2369)
                     /locus_tag="VFD08_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10199.1"
                     /translation="MIQKLLDQIQSNDVKNINFNLDEDKIILELEDNSSVTFKDVSSF
                     VFTDDHAIEDPHMVPIIYEAGGVDAVILRLDDLDGGLSIPNFSICHKDKSMLIEAGSL
                     EIFGSTYQLNQISH"
     gene            2652..4595
                     /gene="metG"
                     /locus_tag="VFD08_03930"
     CDS             2652..4595
                     /gene="metG"
                     /locus_tag="VFD08_03930"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZK10200.1"
                     /translation="MNEGVKMKEKFYITTAIAYTSKIPHIGNTYEWILSDAVARYKRN
                     RGYDVYFLTGTDEHGEKIEQEAKKEGKSAQEHVDLIAKEIQDIDDKLGVSYDQFIRTT
                     DKKHKEIVQKIFRKIYEKGDIYKGFYEGLYCVPCESFFMESQLADGMCPDCGREVVST
                     KEDAYFFKLSKYQKRLEDHIEANPDFIVPPSRKNEMLNNFIRPGLRDLCVSRSSFSWG
                     IPVDFDEGHVVYVWIDALSNYITALGYDPFGESDELFTKNWPADLHIIGKDIVRFHTI
                     YWPIILMSLDLPLPKQVYGHPWLLSDSGKMSKSKGNTLYARDLVKTFGLDRVRYSVLR
                     EMPYSEDGHFSPETLVRRSNSDLSNVLGNLLNRTLAMSHKYFGGQVKLGSPYEEIDLR
                     LQEEVKKTLALYEKKMDSLHISEAMASVIQLAKAGNKYIDDTTPWLLAKDETKKDRLS
                     TVLYSLIENLRIIAILLEPFIPETSEEILNQIRTQIRDYDSIYTYGSLEEDLIVGKAQ
                     PIFERLDLDEIVEKEEAAEKEKVQVKEEAAFKTKEEIDFQTWEKLDLRVAKIISVEDH
                     PQADRLYVLKVDLGQDQRQVVSGIKENYTKEELLGKTVVLLVNLKPIKLRGVLSQGML
                     LAESKGKDVRLLEASLKVGSPIS"
     gene            4605..>4915
                     /locus_tag="VFD08_03935"
     CDS             4605..>4915
                     /locus_tag="VFD08_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZK10201.1"
                     /translation="MKLFDSHAHIDDRRLDNKYLAKKLKDAGIGGVIIPGVDRKGWKR
                     ILEMAKEEDIYYPTLGLHPQEASKTTPEVLEELERLVRDNPQVVAIGEIGLDLHWRKD
                     NL"
BASE COUNT         1535 a          978 c         1060 g         1342 t
ORIGIN      
        1 atgataggtc actgacctat catttttttt agcccctgcc ttgtttgatt ttttcaacaa
       61 ggcctacagc gcctttatag gctgattctg tgcccttggc tgcgatatca gagatacgat
      121 ctacattgcc cataatggag ggcgtaattt cgagtatctc atctatgtgt tcacgttttt
      181 cgtctacaac agttcgaaga agcttaacag ataaaagtac ttcccgtaag acgaggatga
      241 tatagacaat aagtactacg agggcaatca aaactgaggc caataagagt tcttttagtg
      301 agaatacaat ttgcgtttcc ataaaatgtc ctttctagag ttggtgtata gccaggccgt
      361 caatggccat ggcagcttcc ataagtagtt caggaagggt cggatgggcg tgtattgtgt
      421 catagagctc ttttacacta agattgtgct ttatggctag ggtggcttca ctaattaggt
      481 ctgatgcgcc gaccccaagt atatgaaccc ctagaatcct gccactcttg tctgcaatca
      541 tcttaacaaa gccctcgggt tcgtccatgg caagggcctt gccactggcc cggtagagga
      601 atttggatgt cttgtaatct accttttcct ttttgaggtc ttcttcattt ttgcccactg
      661 aagcaatctc aggataaaca aaggtacagg aaggaacagc tctatagtct aacttctgcg
      721 gattaccact taggcgctct acaatattaa gggcctcgta gcttgctaga tgggccaaca
      781 tatctccccc aattacatct cctgcggcat atatatgggg ctggcttgtt ctaagctcat
      841 catcaacaag aatcccctgg ggcttgtaat tgatgccagc agcctcaaga ttacaagaga
      901 aatttggctt tcttccagca gaaagtaata ccttatcaca gaaaacttcc ttgggtcctt
      961 ttttggtctc cactgtgacc ttaagaccct gaggagtttt ttctatcgac tgcattcgtc
     1021 caccaagata gaacttcata ccctggcgtc gtaagtaatt ttgggcgcgt ttttgtatct
     1081 ctaggtcttc tctttttaaa aaggttctac ctgatacagt cactttactg ccaaattcta
     1141 ggaagataga agcaaattca agcccaacta tgctggctcc tgatacaaaa agatgttctg
     1201 gtagttcttc tagctcaagg agctggtcac tacctaaaac ccctggcaaa tcagagccag
     1261 gaatgggggg attaaaggcc acagaccctg tggcaactaa aaagtttttt gcccggtaga
     1321 gctgattatt tacttctatg gttgttgaat caacaaaggc cccccggccc ttgatcacgg
     1381 gaatttcttt tttcttaaac ataagctcaa tgccactaat gagttggtcg gtgattttat
     1441 cttttcgctg cttgaccttg gcataatcaa aagtgtagcc atcaatggtt attccatagg
     1501 ccttaagatt tttggcagta cgaaacactt ctgcattttt taccaggctt ttagtaggga
     1561 tacagcctct attaaggcag gtgcctccca gacgatcttc ttcaataaga agtacatcca
     1621 ggccacgatc cttggccctt agagccgctg catatccgct aggtccgcca cctataatga
     1681 tcaaatcttt catgcaaacc tccttcaaca ttaacaagta tagcataaaa gcactgggct
     1741 atggtatcct taagccatga attatattta tattttacgg tgtggggatg aaagcctcta
     1801 tacaggctgg accaataatc tagaaaaaag aatagacact cataggaaag gaaagggtgc
     1861 aaagtacacc cgctctcgcc ttcctgtgag tcttgtttac tttgagagct ttgataggcc
     1921 aggcgaggca agaaaacgcg aagcagccat aaaaaaaatg tccagaaaag aaaaactagc
     1981 actaattgaa ggcaaagaaa aaaagacccc gaaaggtctt aatgactgat ctggtttagt
     2041 tggtaggtgc ttccaaatat ttctaggctg cctgcttcta ttaacatgga cttatcttta
     2101 tggcagatgg aaaagttggg tatactaaga ccgccatcta ggtcgtctag acgtaggatg
     2161 acagcatcaa ctcctccagc ttcatagatg ataggaacca tatgaggatc ttcaatggcg
     2221 tgatcatcgg taaaaacaaa actggagacg tccttgaagg tgacagaaga attatcctca
     2281 agttcaagga tgattttatc ttcgtctaag ttaaagttga tgtttttcac atcatttgat
     2341 tgtatttgat ctaataattt ttgtatcatt taacactcct gtacaatcta tatcacaaaa
     2401 aaatccaagt gacaagtctc ttttgacaaa gtttcattaa aatgataaga ttatttccac
     2461 aagcgttgaa ggaaagagaa ctttcaaaaa tccttagaga actgatgttt ggtgaaaatc
     2521 agtgattaga gaaagggaag atggctcctg agcagctacg gtttgggccc gttactaccc
     2581 ctaaagctga taagtcagaa aatagggtgg taccgtggac tcttcgcccc tagcgagggg
     2641 ttttttttat tatgaatgaa ggagtgaaga tgaaagaaaa attctatatt acaacagcca
     2701 tagcctatac atccaagatc ccccatatag gaaataccta tgaatggatc ctctcagatg
     2761 ccgtagctcg gtataaaaga aatcgaggct atgatgtcta ctttttaacg ggaaccgatg
     2821 agcatggaga aaaaattgag caagaagcaa aaaaagaagg aaaatcagcc caggaacatg
     2881 tagatctaat tgccaaggaa atccaagata tcgatgacaa gcttggggtt agttatgatc
     2941 agtttattcg caccacagat aaaaaacaca aagaaatagt acaaaagatt tttagaaaaa
     3001 tctatgaaaa aggagatatt tataagggct tctatgaagg cctttattgt gttccctgtg
     3061 aatccttttt tatggaatcc cagctagctg atggcatgtg tcctgattgt ggaagagagg
     3121 tagtctcaac aaaagaggat gcttactttt ttaaactaag taaataccaa aaaagactag
     3181 aagaccatat tgaggccaat ccagacttta ttgtaccccc ctcaagaaaa aatgaaatgt
     3241 taaacaactt tatacgacca ggcctaagag acttatgcgt ttcaagaagc tccttttcct
     3301 gggggattcc cgtggatttt gatgagggac atgttgtcta tgtctggatt gatgccctaa
     3361 gcaattatat tacggccctg ggctatgatc cctttggaga aagcgatgaa ctttttacca
     3421 agaattggcc tgctgattta cacattattg gaaaagacat tgtgcgcttt catactattt
     3481 attggcccat tatcctaatg agcctagatt tacccctgcc caagcaggtc tatggccacc
     3541 cctggctcct aagtgatagt ggaaaaatga gtaaaagtaa gggcaatacc ctttatgcta
     3601 gggatttagt aaaaaccttt ggcctggata gggttcgcta tagcgtttta agagaaatgc
     3661 catattctga agatgggcat ttttccccag aaaccttagt tcgacgaagt aatagcgacc
     3721 tatccaatgt tctaggaaat ctcttaaatc gcaccctggc tatgagccat aaatactttg
     3781 ggggccaggt taaattaggt agcccctatg aggaaattga tcttaggctg caagaagagg
     3841 taaaaaagac ccttgctctt tatgaaaaga aaatggacag ccttcatatt agtgaagcca
     3901 tggccagtgt aattcaactt gccaaggcag gaaacaaata tatagatgat accaccccct
     3961 ggctgctggc aaaggatgag acaaaaaaag ataggctgag tacagtttta tactctttaa
     4021 ttgaaaactt acgaattata gccattttgc tagaaccttt tatcccagaa acctccgagg
     4081 aaattctaaa tcaaattaga acacagatca gggattatga tagtatatac acctatggat
     4141 ctttggaaga ggaccttatt gttggcaagg cccagcccat atttgagcgg cttgacctag
     4201 atgaaatagt ggaaaaagaa gaggcggcag aaaaagaaaa agttcaagta aaagaagaag
     4261 ctgcctttaa aacaaaagaa gagattgatt ttcagacctg ggaaaaactg gatctacggg
     4321 tggcaaaaat cattagcgta gaagatcatc cccaggcaga ccgcctctat gtactaaagg
     4381 tcgatctggg acaagaccag cgtcaagttg tttctggaat aaaagaaaat tatacaaaag
     4441 aagaactttt gggaaaaacc gtcgtactac tagtaaatct aaagcccata aaactcagag
     4501 gagttcttag ccagggtatg ctcctagctg aaagcaaggg aaaagatgta cgactcttag
     4561 aggcaagcct aaaggtaggc agccctatta gttaggagga taaattgaaa ttatttgatt
     4621 cacatgccca tattgatgat agaaggctgg acaataaata tctagctaaa aaactaaagg
     4681 acgctggcat tggaggcgtt attatccctg gagttgatag gaagggatgg aagcgcatac
     4741 ttgagatggc caaggaagag gatatctact atccaaccct gggactgcat cctcaggagg
     4801 cctctaaaac cacacctgag gtcttagagg agctggaaag acttgtaagg gacaatccac
     4861 aggttgtagc cataggtgaa ataggcctgg acctacactg gagaaaggac aatct
//
LOCUS       DASRNA010000144         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_711907,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000144 DASRNA010000000
VERSION     DASRNA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711907"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1686
                     /locus_tag="VFD08_03940"
     CDS             58..1686
                     /locus_tag="VFD08_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10202.1"
                     /translation="MSAQLYVLFIFGALLYYWAKNIRKNKKRLEELKENFGLLGRSRY
                     KSEDLDVHYLQKKENTGKPRIDSITWDDLEMEDFLLKTQCFYSSLGAAHIYESFHYLE
                     EEEVLSARKKLIAMEKKDRECTIKHLFYLGSESGVNLPFFIQEATEFFLPYWLLQGLF
                     YLIILALLALVFLKGLGLVFFGMIVLINVIIYARGTKKIRSQLHNVNYFQRMLKSARH
                     LEKELSQDLVDFKKELQEALAPFKGLRISPLGQYTNTSELSVIRENMEVFFLSSLRQY
                     TKIAGLLEKNRAESERVMELLGELELALYVQALKENKTMCKADFVEEKELSFDGLYHP
                     MVEDAVAYSRKKTKRLLVTGSNASGKSTLMKSMALSVLFSQVTGYAFAKRFSLHKGEL
                     LSSMALRDSIEKKESYFVTEIKSLRRILEKTKESFSYCFIDEILRGTNTIERIAASSS
                     VLESLAKTKSCIYVASHDIELTELLTDYSNVHFREKYLGDDIVFDYKLLEGPSESTNA
                     ILLLKVMGFGDEIILEAQSRVENFLDTRSWRKGI"
     gene            1683..2714
                     /locus_tag="VFD08_03945"
     CDS             1683..2714
                     /locus_tag="VFD08_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZK10203.1"
                     /translation="MNKLVKGLLTVIVGLFLFLGFFTLVEYRPKDIEEIQITSPKALD
                     KKEVTILSYNIGYAGLGKNEDFFMDGGEKVRPQSKDVVEDYLRGLAEEIKAHPADFYL
                     IQEVDLDSKRSYGINQKDFFEKQLGLEGNFAYNYKALYVPYPFPTIGRVNSGLVSLSS
                     YKPQLAYRKSLPVPFSWPVRTVNLKRCLLVEEYEIEGSDKNFILINLHLEAFDDGEGK
                     ILQSRELLRLAQEYYEQGHYVLVGGDWNQTFPGGEIYPLLSEEMWVPGTLIAEDLPPG
                     WRYASDPTSPSCRSGDKPYDQGQEHQVYLIDGYLLSPNLEELSIKTLDYGFSSSDHHP
                     VELRVKLKE"
     gene            2861..>3841
                     /locus_tag="VFD08_03950"
     CDS             2861..>3841
                     /locus_tag="VFD08_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZK10204.1"
                     /translation="MNKLLKGFFSMIVGLFSLLGIFTLLEYRPRDKEDLQITSPKSLD
                     KKEVRILSYNIGYASLGKGQDPFIKRGKKPRPQSKDVVQNYLGGLAEEIKNHPADFYL
                     FQEVDLDSKRSYGINQKEFFERELGLEGNFAYNYKLFYLPLNTGRIRSGLLSLSSYRP
                     QQAYRKSLPQPFCWPLRMIRLKRCLLVEEYEIEDSHKNFILINLHIEASDKGEGKILQ
                     TRELLRLAQEYYKQGHYVVAGGDWNQTFPGGEIYPQLSKEVWMPGTLLKEDLLPGWRY
                     VSDPTTVSARSGEKPYKQDQVYLIDGYLLSPNLEELSIQNLDYDFFFFFFY"
BASE COUNT         1215 a          741 c          838 g         1047 t
ORIGIN      
        1 ggttaccctt ttaaaggcta tggaggggcg taaaaccctt tagcactagg agtatttatg
       61 tctgcccaac tatatgtatt atttattttt ggagccttat tgtattattg ggccaaaaat
      121 attcgaaaaa ataaaaaaag actggaagaa ctaaaggaaa actttgggct tcttggaaga
      181 agtcgctata aaagcgagga cttggatgtc cactacctgc agaaaaaaga aaatacaggt
      241 aagcccagga tagactctat tacctgggac gatcttgaga tggaggattt tcttctaaaa
      301 acccagtgtt tttattcttc cctgggagcg gcccatatat acgagagttt tcattattta
      361 gaagaagagg aagtcttatc cgctagaaaa aaacttattg ccatggaaaa gaaagatcga
      421 gaatgcacca taaaacatct tttctatctt ggtagcgagt caggggtgaa tcttcctttt
      481 tttattcaag aagcaacaga gttttttcta ccctactggc ttttgcaagg acttttttat
      541 ctgattattc ttgcccttct tgctcttgta tttttaaagg ggctgggtct agtttttttt
      601 ggaatgattg tgcttattaa tgtcattata tatgctcggg gtacaaaaaa gattcgcagc
      661 cagctccata atgtcaacta ttttcaaaga atgctaaaaa gcgccaggca tttggaaaaa
      721 gaacttagcc aagatctagt ggattttaaa aaggagttac aagaagctct agctcccttt
      781 aagggccttc gtataagccc cctgggtcaa tataccaata catcagagct ttctgttatt
      841 cgagaaaata tggaagtgtt ttttcttagc tctcttcgtc aatacactaa aattgcggga
      901 cttcttgaaa aaaacagagc cgagtcagaa agggttatgg aacttctagg agaacttgaa
      961 ctggcccttt atgtccaggc cctaaaagaa aacaaaacca tgtgtaaggc cgactttgtt
     1021 gaagaaaaag agctaagctt tgatggtttg tatcatccta tggtggagga cgccgttgct
     1081 tatagtagaa aaaagaccaa aagactcctt gtaacaggaa gcaacgcctc aggcaaatcc
     1141 accctaatga agtccatggc ccttagtgtt cttttctcac aggtaacagg atatgccttt
     1201 gcaaaaagat ttagcctaca taagggagaa ctccttagca gtatggccct acgcgatagc
     1261 atagaaaaaa aagaaagtta ttttgtaaca gaaatcaaaa gtctcagaag gattttggag
     1321 aaaacaaaag aaagtttttc ctattgtttt attgatgaga ttctaagagg caccaatacc
     1381 atagaacgca ttgcagcctc ctcatcggtg ctagagtctc tagcaaagac aaaaagctgc
     1441 atctatgttg ccagccatga tattgaactt acagagctcc taacagatta cagcaatgtg
     1501 cactttagag aaaagtatct aggagatgat attgtttttg actacaaact cttagaggga
     1561 ccatcagaat ccaccaatgc tattttacta ttaaaggtta tgggttttgg ggatgaaatt
     1621 atacttgaag cacaaagtcg ggtagaaaac ttcttagata caagaagctg gaggaaaggg
     1681 atatgaataa actagtaaag ggacttctca cagtaattgt tggcctgttt ttatttctgg
     1741 gtttttttac cttggtggag tatcgcccca aggacataga ggagattcag attacaagtc
     1801 caaaagccct agataaaaaa gaagtcacaa tattaagcta caacataggt tatgcaggct
     1861 tgggtaaaaa cgaagacttt tttatggatg ggggagaaaa ggtaagaccc cagtcaaagg
     1921 atgttgtaga ggactatctt aggggcctag cagaagaaat aaaagcacat ccagcagatt
     1981 tttatctgat ccaggaagta gatcttgact caaaaagaag ctatggcatc aaccaaaaag
     2041 atttttttga aaagcagcta ggcctagagg ggaactttgc ttacaattat aaggccttat
     2101 atgttcccta tccttttcct accataggaa gggtaaactc aggcctggtc agtctttctt
     2161 cctataagcc ccagctggcc tacagaaaaa gcctgcccgt tcccttttcc tggccggtac
     2221 ggacggtaaa tttaaaaaga tgtcttcttg tcgaagaata tgagattgag ggtagcgata
     2281 aaaactttat cctaattaac ctgcatctag aagcctttga tgatggagaa ggaaaaatcc
     2341 ttcaaagtag agagcttcta cgcctagccc aagagtacta tgagcagggc cactatgtac
     2401 tagttggggg agactggaat caaacctttc ctggagggga gatttatccc ctgctgagtg
     2461 aagagatgtg ggtgccagga acccttattg cagaagatct tcctcctggt tggcgctacg
     2521 cgagtgatcc caccagcccc tcttgtcgct caggagacaa accctatgat caggggcaag
     2581 aacatcaagt gtacctaata gatggttatc ttcttagtcc caacctagag gaactttcta
     2641 ttaagaccct ggattatggc tttagcagta gtgatcatca tccagtggaa ctaagagtaa
     2701 aacttaagga ataaaaagag gcttctgcct agggcaggag cctttttaac aagtgctatt
     2761 atacttttaa gctataggct ttggggatga agttatactc aaagcacaaa agcaactaga
     2821 aaacttctta gaaagaagaa gcggcagaag ggacaagggt atgaataaac tgttaaaggg
     2881 atttttttca atgattgttg gcctattttc cttgctaggt atttttacct tgctagaata
     2941 tcgccccagg gacaaagagg accttcaaat tacaagtcca aaatccctgg ataaaaaaga
     3001 agtcagaatt ttaagctaca acataggcta tgcctcccta ggaaaaggcc aagacccttt
     3061 tataaagagg ggaaaaaagc ccaggcccca gtcaaaagat gttgtgcaga actatcttgg
     3121 ggggctagca gaagaaataa aaaatcatcc agcagatttt tatttgttcc aggaagtaga
     3181 tcttgactca aaaagaagct atggcatcaa ccaaaaagag ttctttgaga gggagctggg
     3241 actagagggc aactttgcct acaattataa gctcttttat cttcctctta acacaggaag
     3301 aataaggtcg ggccttctaa gcctttcttc ctacaggccc cagcaggcct accgaaaaag
     3361 cctgccccag cccttttgct ggcccctaag gatgatccgt ttaaaaagat gccttctggt
     3421 ggaagaatat gaaatagagg acagccataa aaactttatc ctgatcaatc tacatataga
     3481 agcctctgat aagggggagg gaaaaatcct tcaaacaagg gagcttctac gcctagccca
     3541 agaatactat aagcagggcc actatgtagt ggccggggga gactggaacc aaacctttcc
     3601 tggaggggag atttatcccc agctaagtaa ggaggtctgg atgccaggca cccttttgaa
     3661 agaagatctt ctccctggct ggcgctacgt gagtgatccc actactgtct ctgctcgctc
     3721 aggggaaaaa ccctataagc aggaccaagt atatcttatt gatggctacc ttcttagtcc
     3781 caatctagag gagctttcca ttcagaacct ggattatgac tttttttttt ttttttttta
     3841 t
//
LOCUS       DASRNA010000145         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_484877,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000145 DASRNA010000000
VERSION     DASRNA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484877"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..462)
                     /locus_tag="VFD08_03955"
     CDS             complement(43..462)
                     /locus_tag="VFD08_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10205.1"
                     /translation="MKKLIAFFLLLVLLVSCSSPSEEEDLESKLAQRYETRRKQIGLV
                     ELIEEDWDRAWLVNSFENNSTLPEELAELEAAQSPGVILKKGEELTSYKLPLDAVFVY
                     PESYSVQAIEMNIGTIFTIEKEGGVYRLLYRLPSSPT"
     gene            complement(649..2292)
                     /locus_tag="VFD08_03960"
     CDS             complement(649..2292)
                     /locus_tag="VFD08_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZK10206.1"
                     /translation="MKRAKDIEDFAIELTKVRSIVGTKEELKATKKVMDQCKKMEYFK
                     KHPEDLFYVDAIDDPLERNSVVALVRGKKGNSKKTVVLIGHTDTVGVSEYGHHIEAYA
                     TNPHKLAQKLSEVTIPEDAKIDLESGEYLFARGIFDMKTGVSTLVHILEELSEDVENL
                     QGNVIFAGVCDEEGGSAGMLSLVPRLVDMMEEEGFDYRALIDTDYSAPRFEGDKSRYI
                     YVGTVGKIMPSFFIVGSESHAGDPYQGLDPNQISSAIVEEMNLSMDYADQAEGEVTVP
                     PVTLRQRDLKREYSVQTAKTAYLYFNFSTHSASPDQIMEKVKKGTKKAFKKVIDSLNS
                     EFKVYSERSGFAYYKLPWKERVLSYSELYRLVKAQSGEEVDQRLKALEEKLLKEEGVD
                     DREYSLRMVELVHSMWKDKNPLVVVFYSPPYYPHIYVKGENKKEKELLQAVAAAQKNL
                     RSDYDIKMRKFYPYISDLSYGAAPREKKALESLKENMPGFGTKYKLPLEEMQKLDLPV
                     VNIGPFGKDAHKFTERVQRDYSYNVAPGLVKDTILNLLK"
     gene            complement(2339..2944)
                     /locus_tag="VFD08_03965"
     CDS             complement(2339..2944)
                     /locus_tag="VFD08_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10207.1"
                     /translation="MNLMNKKFLAAFAFIFLALVFLFWPETKGPKEPLDINVAEVEQG
                     KAEEETLITEKEEQIEKISGSYGGQVNVDSIELVGKNIFYKVPVALAKNELEEQLAEG
                     FYVNLIITEDGAWTLDLGDTFAQYQITTIGSTQEDRIDMEEGSFEAERGFFVPDLDDN
                     IYLTLRGHVLDSVQGTILLEGDTPNIKLSFLGSFNLIKEEE"
     gene            complement(3040..>3438)
                     /locus_tag="VFD08_03970"
     CDS             complement(3040..>3438)
                     /locus_tag="VFD08_03970"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HZK10208.1"
                     /translation="LSFGPFQFRIAEFLNLLAFYHPGFIFPISLACAISNLFSPFGIV
                     DVVLGTLHTYVSLKAMTYVKKDWIAALFPGLFAFIIGAQITFFSQIPLSFFLITGQIM
                     ISEFIIGLLSVPFYRGKIGGVRLKDYLKDV"
BASE COUNT          979 a          748 c          564 g         1147 t
ORIGIN      
        1 ctctcttttt tccttgctcc ctacaggagc attttttctt tatcaagtag ggctgcttgg
       61 cagtctatat aataatcggt agactccccc ttctttttca atggtaaata tcgttcctat
      121 attcatttct attgcctgaa ccgagtagga ttctggataa acaaataccg catcaagggg
      181 taacttgtag ctagtaagtt cttctccttt tttaagaatc actccagggc tttgtgctgc
      241 ttctagttct gccaattcct ctggcaaggt agaattattc tcaaaactat tgactagcca
      301 ggctcgatcc cagtcttctt cgattaactc aactaaaccg atttgcttac gtcttgtctc
      361 gtagcgctga gcaagtttac tttctaagtc ttcttcctca gaagggctac tacaagaaac
      421 aagcaggacc agtaaaagaa aaaatgctat tagctttttc attttacctc ctttttctct
      481 gctacctcca tatataccca tttaaggggg tcttctaccg tttttttatt aacaaggact
      541 ctttagctcc ttatccccca tggtttagag caaattacaa ggggctttgt agctagaaga
      601 tgatagcctc tttaaccaat gccacaaggt tttataaatg aaatcagttt attttagtag
      661 atttaaaatc gtatccttaa caagccctgg ggcaacatta tatgagtaat ctctttgcac
      721 cctctcagta aatttgtggg catcctttcc aaagggccca atatttacta caggtagatc
      781 gagtttttgc atctcttcta gaggaagttt gtactttgtg ccaaagccag gcatattttc
      841 ttttaggctt tctagggctt ttttttctct aggagctgct ccataactta gatccgagat
      901 ataaggataa aatttgcgca tctttatatc atagtcgctc cttaggtttt tctgggcagc
      961 agcgactgct tgaagaagct ctttttcctt tttattttct ccctttacat agatatgggg
     1021 ataataaggt ggcgaataaa agactactac aagaggattc ttatccttcc acatgctgtg
     1081 aactagctct accattcgaa gggaatattc tctatcatcg actccctctt cttttagtag
     1141 cttttcttct agtgctttaa gtctttgatc tacttcctcg ccactctggg cttttactag
     1201 cctatagagt tcactataag aaagaaccct ttctttccag ggaagcttat aataggcaaa
     1261 gccgctacgc tcagaataga ctttgaactc gctattaaga gaatctatta cctttttaaa
     1321 ggccttcttt gttccctttt ttactttttc cattatttga tctggactag cagaatgggt
     1381 agaaaaatta aaatacagat aggctgtttt ggctgtttgt acagaatatt ctctttttaa
     1441 atccctttgg cgtaaagtaa ctggtggtac agttacttcc ccctctgctt gatcggcata
     1501 atccatagaa agattcattt cctctacaat ggccgaggaa atttggtttg ggtccagccc
     1561 ttgatagggg tccccagcgt gactttcgct gcccacaata aaaaagctag gcataatttt
     1621 ccctacggta cctacataaa tataacggct cttatcccct tcaaaacgag gcgcagagta
     1681 gtctgtatca ataagggctc ggtaatcaaa cccctcttct tccatcatat ctaccaagcg
     1741 aggtacaagg ctcagcatac cagcagagcc gccttcttca tcacaaactc cagcaaaaat
     1801 aacattgcct tgaagatttt caacatcttc tgataattct tctaaaatat gtactaaagt
     1861 agagactcct gttttcatat caaaaatccc cctagcaaat agatactctc cactttctaa
     1921 gtctattttg gcatcctctg gaatggttac ttccgatagc ttttgtgcaa gcttatgggg
     1981 gtttgttgca taggcctcaa tatggtggcc atattctgat actccaaccg tatctgtatg
     2041 gccaatcaaa accacagttt ttttactatt tccttttttg ccacgaacaa gggctactac
     2101 agaatttctt tctagtggat catctatggc atccacataa aatagatcct ctggatgttt
     2161 tttaaagtac tccatctttt tgcattgatc cattactttt ttggtagcct taagttcttc
     2221 tttagtgccg acaatgctcc ggacttttgt gagttctatg gcaaaatctt caatgtcttt
     2281 tgctcttttc ataaacacct aacctttact gtataattag caagctcttg tggatttttt
     2341 actcttcttc ttttatcaag ttaaaagaac caagaaaaga gagttttata tttggagtat
     2401 ctccttctag aagaatcgtt ccttgtacag aatcaagaac atggcccctt aaggtcaagt
     2461 agatattgtc atctaagtct gggacaaaaa aacctctttc tgcttcaaaa gaaccctcct
     2521 ccatatcaat cctatcttcc tgggtagaac caatggttgt aatttggtat tgtgcaaagg
     2581 tatcgcctaa atccaaggtc caggccccat cctctgtaat aataagattt acataaaagc
     2641 cctcggctag ttgttcttct agctcatttt ttgccagagc aactggtacc ttataaaaaa
     2701 tatttttccc cacaagttca atagagtcca cattcacttg acctccataa cttccggaaa
     2761 tcttttctat ttgctcttct ttttctgtaa taagagtttc ttcctcggct tttccctgct
     2821 caacttctgc tacatttata tccaggggct ccttgggtcc ttttgtttct ggccaaaaaa
     2881 gaaagacaag agcaagaaaa ataaaagcaa aagcggctag aaatttctta ttcattaaat
     2941 tcatttttta ccccccccca aggaaaacag tctttttttt ataaaagttt tattcctact
     3001 aaacttttat accccttatt ttatctattc cgacaaaatt tatacatctt ttagatagtc
     3061 ttttaatctt acacccccaa tttttccgcg atagaaaggg acacttaaaa gacctataat
     3121 aaattctgaa atcataattt gccctgtaat aaggaaaaaa ctcaagggta tttgagaaaa
     3181 aaatgtaatc tgtgcaccaa taataaatgc aaatagccca ggaaaaagtg ccgcaatcca
     3241 gtctttttta acatatgtca tcgcctttag acttacatag gtgtgaagag tgcccaagac
     3301 aacatcaaca atgccaaagg gactaaatag attgcttata gcacaggcta ggctaatagg
     3361 aaaaataaaa cctggatgat aaaatgcaag aagattaaga aattctgcta tacgaaactg
     3421 aaagggacca aagcttaa
//
LOCUS       DASRNA010000146         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_194869,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000146 DASRNA010000000
VERSION     DASRNA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194869"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFD08_03975"
     CDS             complement(<1..862)
                     /locus_tag="VFD08_03975"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZK10209.1"
                     /translation="MKWIFSVDKKWKIGLNNDLLVRIPEDLSRFKEITMGQILLMGRK
                     TFISLKEGQPLPGRDHIVLTKDKSFSAPGVYLARSKSEALARIEKLLAEEKREVFLIG
                     GESIARQFIDQTSEAYITHIEASFKADTSLPNLLEEGFVLIDKSPLYQGDYDYYYAHY
                     KRKDTDMDRSWDLKKIFRTDEEFLEEVASLKKDAVKIGEFKGRLLESSSTLNKALKTR
                     EDLFRRFEKLWVYGHLNHDSDTRISRYQNYQAQLRGVQAELSSMAAYFEPEILTSSWD
                     TIDRFIEEDKD"
     gene            complement(874..1593)
                     /locus_tag="VFD08_03980"
     CDS             complement(874..1593)
                     /locus_tag="VFD08_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZK10210.1"
                     /translation="MGRIGNIIQRKSKQDSKKAGLFTKLGRNIMVAAREGGPDPEYNS
                     SLESAIEKAKAANMPNDNIDRAIKKGAGGLEGAEFIQIHYEGYGPSGVALLVECLSDN
                     VNRTAGDIRSYFSKCGGNLGTNGCVSYLFDHAGVLVIDKEDSMEEDEMMMLALEAGAE
                     DFISEEGHFEIYSSVEDFGRVRDQLKNQGFSFSMAELRYLPQNSVRLEKEEDQKNMQK
                     LMDFLEDNDDVQNVYSNWDEA"
BASE COUNT          519 a          533 c          413 g          736 t
ORIGIN      
        1 ggtccttatc ttcttctata aagcgatcaa tggtatccca ggaggaagtg aggatctctg
       61 gctcgaaata ggccgccatg gagctaagtt cagcctggac ccctcgcagc tgggcctggt
      121 aattctggta gcggctgatg cgggtgtcac tatcgtgatt taggtggcca tagacccaga
      181 gcttttcaaa gcggcgaaaa agatcctccc tagtttttag ggccttattt aaagtagaag
      241 aagactccag cagtcttccc ttaaattcac cgatctttac cgcgtccttt ttaagggagg
      301 ccacttcctc taaaaactcc tcatcagtcc taaatatctt ttttaagtcc cagcttctat
      361 ccatatctgt gtcctttctt ttatagtggg cataataata atcatagtcg ccttggtaaa
      421 ggggactctt atctataagc acaaatcctt cttctagtaa attaggaaga gaggtatctg
      481 ctttaaagct agcctctata tgggtaatat aggcttcact tgtctggtca ataaactggc
      541 gggcaatgct ttctccccca ataagaaaga cttctctttt ttcttctgct agaagttttt
      601 ctatccgggc cagggcctcg cttttgctac gggcaagata gactccgggg gcagaaaaac
      661 tcttatcctt tgttagcaca atatggtctc gcccaggcag gggctgcccc tccttaaggg
      721 agataaaggt ttttcttccc attagaagaa tctggcccat ggttatttcc ttaaagcggc
      781 taaggtcttc agggattctt actaatagat cattatttag tccgatcttc cacttcttat
      841 ctactgaaaa aatccatttc attttttctc cccctaagcc tcatcccagt tgctatagac
      901 attttgtaca tcatcattgt cttctaaaaa gtccattagc ttttgcatgt ttttttggtc
      961 ttcttctttt tctaatctca cactgttttg gggaaggtag cgaagttcag ccatgctaaa
     1021 gctaaaacct tgatttttta gctgatctct taccctgcca aaatcttcta cactgctgta
     1081 gatctcaaag tggccctctt cggaaataaa gtcttctgct cctgcttcta gagccagcat
     1141 catcatctcg tcttcttcca tggagtcttc cttatcaatg accagaaccc ctgcatgatc
     1201 aaataggtag ctaacgcagc cattggtccc tagatttcct ccgcatttgg agaagtaact
     1261 tcttatatcc ccggccgttc gattgacatt atcacttagg cactctacta gaagggccac
     1321 cccactgggg ccatagccct catagtggat ctgtataaat tcagccccct ctagccctcc
     1381 ggcccctttt tttatggccc tatcaatgtt atcatttggc atatttgcag ccttggcctt
     1441 ttctatggca ctttctaggc tggaattgta ttcaggatca ggcccacctt ccctagctgc
     1501 caccataatg ttgcggccca gcttggtaaa gagtcctgct ttttttgaat cttgtttgct
     1561 ttttctttga atgatatttc caatacgtcc catattaaac ctacctttct tttccattgt
     1621 agcatgctag aagggttaaa aaaagtacaa gaaaagcaat gaccctatca acttattgta
     1681 ggcttataag tataaacaag aaagatcaag ttatatctct aacagatata tccacccctc
     1741 ttaaccccac taatgtttgc gcttattatc ccacttaaaa gccactttct ggaactcttc
     1801 cgctctaggc ctcagccttt ttcttcttag aagtagtctt gctttgagca gctgtcttag
     1861 cttgagtaga ggatttcttt gcaggttttt ttggcttctt ttatctcaga ctggcctctt
     1921 tatcttgctg cgtaatttct ggtatctgtg cggtagtgcc cccattaacc tttatgcagt
     1981 tattttacca gggtttttgc ttggtctata taaagaccgt tttctgggag tactctgcta
     2041 gctcagcttc cttcatcttc agttttttct ggcaagaaga aattcatctt gccagtccat
     2101 ctacctgcgg gtataacttt tataaaaaga gagtaccctt atctgtaagc tcctggcgct
     2161 agcttcttag agctgcatct tctctagggg tgtgtttact c
//
LOCUS       DASRNA010000147         8233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1090184,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000147 DASRNA010000000
VERSION     DASRNA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090184"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..239
                     /locus_tag="VFD08_03985"
     CDS             27..239
                     /locus_tag="VFD08_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK10211.1"
                     /translation="MSKENLVLHLNRDTCKGCGICVAFCPTNVLEMAEDNKCEIINIE
                     KCIKCGLCELRCPDFAIYLEEDKDEK"
     gene            229..1365
                     /locus_tag="VFD08_03990"
     CDS             229..1365
                     /locus_tag="VFD08_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZK10212.1"
                     /translation="MKNKALLMQGNEASVRGALYAGMSFYSGYPITPSTEIAEISAEL
                     LPKVGGTFIQMEDEIAGIATAIGASIAGRKVMTATSGPGFTLKQENLGFAVMAEIPLV
                     IINVQRGGPSTGLPTQSAQGDVMQAKWGTHGDSAIIALTPNSVTETFYLTVRAFNLAE
                     KYRTPVILLTDEIIGHAREKIILPKPGDIEIYNRLTPKEDQPYRPYGVEEGDIVPRFA
                     PFGTGHRYNITGLFHDEQGFPSNSQENAKNMLQRIHNKLADEAIIDYESLYCDDAEFV
                     FLAYGSTSRSALAGIKQLRSEGYKVGLFRPITLWPSPEKAIKKLASHVGEICVAEMNL
                     GQYSLEVQRIAQGSTKVSHMGRANGEILLPEEFVARAKEYFNER"
     gene            1355..2179
                     /locus_tag="VFD08_03995"
     CDS             1355..2179
                     /locus_tag="VFD08_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZK10213.1"
                     /translation="MSDRSKLVNKYFKVENLPHIWCPGCSHGIVTRAITSAIDQLDLD
                     PEKIVVVSGIGCSSRAAGYLNFNTLHTTHGRAIAYATGIKLANPELHVIVISGDGDAS
                     AIGGNHLIHSCRRNINLTVIIFNNNIYGMTGGQYSPTTPHSALGTTVPYGNIDRPFDL
                     VRLVEGAGATYIARGTAYHTLQLTRYIKNAISHKGLSVVEAISTCPTYFGRKNDIGNA
                     VDMMRWLKDSFVEKRAYEKLEEEKKKNKFPFGEFVNIQAPEYTQVLAEMIKSVEVK"
     gene            2179..2730
                     /locus_tag="VFD08_04000"
     CDS             2179..2730
                     /locus_tag="VFD08_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZK10214.1"
                     /translation="MKQLEFIFSGTGGQGVILLGVIMAETAINEGMNALQSQSYGPEA
                     RGGASRCEVIISQEEIFFPKVENPDYILCISQAAADKYAYGLSKDTVLILDDRIELSK
                     KTGTEKIYQLPILSTAHKKIGKEVTANIVSLGVLLEITKLFSKEAIIHSVLNRVPKGT
                     EKINTQAVEMGIGLVRKWQKKNA"
     gene            2723..3568
                     /locus_tag="VFD08_04005"
     CDS             2723..3568
                     /locus_tag="VFD08_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HZK10215.1"
                     /translation="MHNTFDKITSQYEKLSKSHQKIADYILQHYDRAVYMTARKIASH
                     LGISESTVVRFATSLGYDGYPSFQEHLRNTVKAVATTREKLSKELEPKSKAAALSQSF
                     QRDLRDLKNTSEIINYPALKLAAKKVADSDRVFILGKRSSGVLVDYLSYYLNFFHDNV
                     RVLESNVLDLFEQLIHIKKGDLIIMFSFPRYAKNTIEMAKFIKEKGGHIIAITDNVDA
                     PIAEYSAHCLIASYSRESFIDSLVAPMALVNALITSIAYDNLELTENKLDSLEILWEK
                     YDVYI"
     gene            3586..4227
                     /gene="cmk"
                     /locus_tag="VFD08_04010"
     CDS             3586..4227
                     /gene="cmk"
                     /locus_tag="VFD08_04010"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZK10216.1"
                     /translation="MIIAIDGPAASGKSSVAKELAKRLGYLYIDTGAMFRAVTFYALE
                     QGLDEEDQLKTRLDKLHLKSIPGGIEIDGRKLYDELRSKDVDLNVSYYSTMASIRSFL
                     KDIQRQEARRHNVIMDGRDIGTAVFPQAQVKIFLSASAEKRAERRFKQRSNSLSYAQI
                     LEDIKRRDKIDSSRNVAPLKQAKDAIFIDSSNLSLEEVINKIEGIVKNVSSNS"
     gene            4208..4798
                     /locus_tag="VFD08_04015"
     CDS             4208..4798
                     /locus_tag="VFD08_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZK10217.1"
                     /translation="MFRRIASVIFNFILRVFYPVEVYGRENISKENFLIIANHGHMFD
                     PFLVNYAFKEHFYIIGKAELFKIPILRKILELADVFPVSRDQLDLGAIKTSIAKLAQS
                     SLLLFPEGTRNHSLDPLEGKSGAIMIASKAEVKILPMVLVGNFKIFRPLKVFILPARE
                     VGEYGFERMNSQAYKSIVDSLLNDIYTKLREETNDH"
     gene            4788..5615
                     /locus_tag="VFD08_04020"
     CDS             4788..5615
                     /locus_tag="VFD08_04020"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZK10218.1"
                     /translation="MIIKKSNPMGFCFGVSRAISMAEKLSKNSPIYCYGSLIHNEDEI
                     ERLAKRGITTINQLDKEMDLPILIRSHGVGKDVYDFLDKHQLEYHDSTCPFVKKIHEH
                     VKSFLSQEKEIAILGDKTHPEIQGIISWGEGKIHVLADMEAVRNLECPKELVLLAQTT
                     LKKEFFDEAVEYLEGKTQLQAINTICSATKRRQEAARELAKEVDLMIIIGGKKSSNTN
                     KLYEICKKVNKNTLYIENLAEIRMQDIKKYGTIGISAGASTPEWVINNIMEHLQNET"
     gene            5630..6682
                     /gene="rpsA"
                     /locus_tag="VFD08_04025"
     CDS             5630..6682
                     /gene="rpsA"
                     /locus_tag="VFD08_04025"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZK10219.1"
                     /translation="MENLSMKEMMETASFSAPKRNEVITGKVVMVKDDGVVVNIGYKA
                     DGILPVDEISNPENLPLEELFNVSDEIVVLVLKRDNGEGDVLLSSKRLRSRKDWETLE
                     EKYKNKESVNVKITEVVKGGLSAHYNDIRAFIPASHVDINFVRDLSQFVGEECEVEFL
                     DFDRRRNQVVLSRKLVVEKERKVELDEFWDSLAIGQIREGVVRRFTSYGAFVDLGATD
                     GLIHVSEIQWGKVNKPSDVLKANETLDFMVLDFSRENNKISLSRKALSPDPWEVIDEM
                     YDVGENYDGKIVSLTDFGAFVELEPGLEGLIHVSQISEDRIDQPKDVLSLGEEVIVRI
                     LDIDKEQKRIKLSMKQ"
     gene            complement(6775..7320)
                     /locus_tag="VFD08_04030"
     CDS             complement(6775..7320)
                     /locus_tag="VFD08_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZK10220.1"
                     /translation="MRLNQKKALVLVIDIQERLLPVISNKEEVEKNTQILLEGADILS
                     LPIVFSEQYPRGLGSTVGSLEKWIREDNLYEKTSFSLMDDLKKPMESLFSQGRNQFII
                     SGIESHVCVYQTARDLLELGKEVIIAYDAVGSRKDDNSQWALKSLQSLGALVLPVETI
                     LFDLQRRSDTASFKSVSKLIK"
     gene            complement(7371..>8233)
                     /locus_tag="VFD08_04035"
     CDS             complement(7371..>8233)
                     /locus_tag="VFD08_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK10221.1"
                     /translation="LSLLTSLIFGFSFIFTKTGTSTVSVLHLLGWRFIIAFIFFELFC
                     RILRIPIKLKKPGWQNLIVLGSLFPVLYFTLESLGIRETTASEAGVIMSIGPIVMMVL
                     SSIFLQEPPRKEQLVGILTSTLGVILMVLTKRNEPSFSFFGYLFLLGGVFSYGFYGIK
                     QRKIKDFTVYERTYAMMLMGAVVFFLASLLIATFQGEVKSFLLLPLVNKRFLLSLVYL
                     SLLSSNVAFLMSVTAIGLVGPTVASSFSGLTTLTSVLAGVFFLNEVFLPQQWAAATMI
                     VLGVFLANRH"
BASE COUNT         2751 a         1447 c         1645 g         2390 t
ORIGIN      
        1 gtatatataa atttaggagg aataatgtga gtaaagaaaa tcttgtttta cacttaaatc
       61 gtgatacttg caaagggtgc ggcatatgtg tagccttttg tccaacaaat gttcttgaga
      121 tggcagaaga taacaaatgt gaaatcataa atatagaaaa atgcattaaa tgtggtttat
      181 gcgaactccg atgtccggac tttgctattt atctagagga ggacaaggat gaaaaataaa
      241 gccttactta tgcagggcaa tgaggctagt gttcgggggg cgctctatgc agggatgtct
      301 ttttattctg gttatcccat aacaccctca acagaaattg ctgaaatatc tgctgagcta
      361 ttgcccaagg tcggtggtac ttttattcaa atggaagatg aaattgctgg catagctaca
      421 gccattggag catctattgc tggaagaaaa gtaatgacag caacatctgg acccggcttt
      481 acactaaaac aagaaaattt aggttttgct gttatggcag aaattccctt ggttatcatc
      541 aatgttcaaa ggggagggcc atcgacgggg ctacccacac agtctgcgca aggagatgtt
      601 atgcaggcta aatggggaac ccatggtgat agtgccatca ttgccttaac tccaaattcg
      661 gtgactgaaa ccttctatct aactgtacgc gcttttaatc ttgctgaaaa gtatcgtact
      721 cccgttattc ttttaactga tgaaatcatt ggccatgccc gggaaaagat cattctaccc
      781 aagcccggag atatcgaaat ttacaatcgt ttgaccccta aagaagacca accctatagg
      841 ccctacgggg tagaagaagg agatatcgtt ccacgatttg ctccttttgg aacaggacat
      901 cgttataata ttacaggcct ttttcatgat gaacaaggct ttcccagtaa ttctcaagaa
      961 aacgctaaaa atatgcttca aagaatccac aataaacttg ctgatgaagc gattatagat
     1021 tatgaaagcc tctattgtga tgatgcagaa tttgtctttt tagcctatgg gtctacttcc
     1081 cggtctgccc ttgctggcat caaacaatta aggagtgaag gctataaagt tggactattt
     1141 cgaccaatta ccctttggcc ttcgccagaa aaagccatta aaaaactagc ttctcatgtt
     1201 ggtgaaatat gtgttgctga gatgaattta ggacaatatt ctcttgaagt tcaacgtatt
     1261 gctcaaggaa gtactaaggt tagtcatatg ggaagagcaa atggtgagat cttacttcct
     1321 gaagaatttg tggcccgtgc aaaggagtat tttaatgagc gatagaagta aattagtaaa
     1381 taaatatttt aaggtggaga accttcctca tatttggtgc ccaggatgca gtcatggtat
     1441 tgtcacccgg gccatcacta gcgccattga tcaattggat ctagatccag aaaagattgt
     1501 tgttgtctct ggtattggtt gctcttctag agcagcaggt tatttaaact ttaatactct
     1561 ccatacaact catggacggg ctattgccta tgcaactggt attaaacttg caaaccctga
     1621 gctgcatgta attgttattt ctggagatgg agatgctagc gctattgggg gaaaccactt
     1681 gatccattca tgcagaagaa atatcaatct tacggtaatc atcttcaata ataatatcta
     1741 cggcatgact ggtggacaat attcaccgac aactcctcac tcggcccttg gtactacggt
     1801 gccctatggc aatattgata gaccctttga tctagtacga ctggtggaag gtgctggtgc
     1861 aacctatatt gccagaggaa cagcctatca taccctgcaa ctaacaagat acattaaaaa
     1921 cgccattagc cataagggac taagtgttgt ggaagccatt tcaacttgcc ccacttattt
     1981 tggtcgtaaa aatgatattg gaaatgcagt agatatgatg aggtggctaa aagatagctt
     2041 tgttgaaaaa agagcctatg aaaaacttga ggaagaaaag aaaaagaata aatttccctt
     2101 tggagaattt gttaatattc aagcccctga atatacccag gttttggctg aaatgatcaa
     2161 aagtgtggag gtcaaataat gaaacagctg gaatttattt tttcaggaac cggtggacag
     2221 ggagtaattt tgcttggcgt tattatggct gaaaccgcaa taaacgaagg catgaatgcc
     2281 cttcaaagtc aaagctatgg tcccgaggct agaggaggag caagtcgatg tgaggtcatc
     2341 atttcccagg aagaaatatt ttttcccaag gtagaaaatc cagattatat cctttgtatc
     2401 tctcaagccg cagctgacaa atatgcctat gggcttagta aagatactgt tcttatttta
     2461 gatgatcgca tcgagcttag taaaaaaact ggcaccgaaa aaatctatca actgccgatt
     2521 ttaagtacag cccataaaaa gattggaaaa gaagtcacgg ccaatatcgt aagtttgggt
     2581 gttcttcttg aaattactaa actcttttct aaagaagcca tcattcattc tgtcttaaat
     2641 cgcgtaccca aaggaacaga aaaaatcaat acacaagccg ttgaaatggg aattggactt
     2701 gtaagaaaat ggcagaaaaa aaatgcataa tacttttgat aaaatcacat cgcaatatga
     2761 aaaactaagt aaatctcatc aaaagatagc tgattatatt ttacagcact atgatcgagc
     2821 tgtttatatg acagcgcgta aaattgcatc tcatcttggt ataagcgaat ccactgtagt
     2881 tcgatttgcc acaagccttg gatatgatgg ctacccatcc tttcaagaac atcttagaaa
     2941 tacggtaaag gcagttgcca caacaagaga aaaacttagc aaggagctgg aaccaaaaag
     3001 taaggctgca gcattaagcc agtcttttca aagagattta cgcgatctta aaaacacttc
     3061 agaaatcatt aattatcctg ccttaaaatt agctgccaaa aaggttgctg actctgatcg
     3121 agtctttatc ctgggcaaac gttcttctgg agttttggtt gactatcttt cctattattt
     3181 aaactttttt catgacaatg tacgtgtgct tgagagtaat gtccttgatt tatttgaaca
     3241 acttatccat ataaaaaaag gcgatttaat cattatgttt agctttcccc gttatgcaaa
     3301 aaatacgatt gaaatggcca agtttattaa agaaaaagga ggtcacatta ttgctataac
     3361 tgataatgta gatgctccca ttgctgagta ttcagctcat tgtcttattg ctagctattc
     3421 tagggagtct tttattgatt ctttggtagc tcccatggcc ttagttaatg ccttgataac
     3481 ctcaattgcc tatgataatc tagagctaac tgagaacaaa ctagatagtc tagaaattct
     3541 ctgggagaaa tatgatgtat atatctagag aggtaattta taatcatgat aattgcaatt
     3601 gacggcccag ccgcatcagg taaatctagc gtagctaagg aactagcaaa gcgtcttggc
     3661 tatctttata ttgataccgg ggccatgttt agggctgtaa ccttctatgc cttagagcag
     3721 ggattagatg aggaagacca gctaaaaaca agattagata agcttcattt aaaaagcatt
     3781 ccaggcggca tagaaattga tggaagaaaa ctttatgatg aattaagaag taaagatgtt
     3841 gatcttaatg tatcttatta ttctactatg gcttcaatta gaagttttct aaaagatatt
     3901 caaagacaag aggctcgtcg ccataatgtc attatggatg gaagagatat tggtactgcc
     3961 gtttttcctc aagcccaggt aaaaatcttt cttagcgcta gtgctgaaaa aagagcagaa
     4021 aggcgattta aacaaagaag caattcccta agctatgcac aaattcttga agatattaaa
     4081 agaagagata aaattgactc ttcacggaat gttgctcctc ttaaacaagc taaagacgct
     4141 attttcatcg atagcagtaa cttgagcctg gaagaagtta taaacaaaat cgaagggata
     4201 gttaaaaatg tttcgtcgaa tagctagtgt gatttttaat tttatcctga gagtttttta
     4261 tccggtagaa gtgtatggta gagaaaatat aagcaaagaa aatttcctta taattgctaa
     4321 tcatggacat atgtttgatc cttttttagt aaattatgct tttaaagaac atttttacat
     4381 tattggaaaa gctgagctgt ttaaaatccc tattttaaga aaaatcttag aactagcgga
     4441 tgtctttcct gttagtcgag atcagctaga tctaggagcg attaaaacct ctattgccaa
     4501 acttgcacag agcagtcttc ttctctttcc tgaggggaca agaaaccaca gcctagaccc
     4561 cttagagggt aaaagtggcg cgattatgat tgcctccaag gctgaagtaa aaattctacc
     4621 tatggtattg gtaggcaact ttaaaatctt tcgaccttta aaggttttta ttcttccagc
     4681 aagggaagta ggggagtatg gctttgaacg tatgaacagc caagcctata aatcaattgt
     4741 tgattctctt ctcaatgata tctacacaaa gctcagggag gaaacaaatg atcattaaaa
     4801 aaagtaatcc tatggggttt tgttttggtg tgtctcgggc gatttccatg gcagaaaaac
     4861 tatccaaaaa tagtcctatc tactgctatg gatcactcat tcataatgaa gatgaaatag
     4921 agcgcttggc taaaagggga attaccacta ttaatcagct tgataaagaa atggatcttc
     4981 ccattcttat tcgctcccat ggggtgggaa aagatgttta tgatttttta gataagcatc
     5041 aacttgaata ccacgattcc acctgtccct ttgtaaaaaa aatacatgaa catgtaaaga
     5101 gttttttatc ccaggaaaaa gaaattgcta tattaggaga taaaactcat ccagaaatac
     5161 agggcatcat tagctggggg gaaggaaaaa tacatgtact agcagatatg gaggcagtaa
     5221 gaaaccttga gtgcccaaag gaacttgtcc tccttgctca aaccacgcta aaaaaagaat
     5281 tctttgatga agctgttgaa tatctggaag gaaaaactca gcttcaagca ataaatacaa
     5341 tatgttctgc caccaaaaga cgtcaagaag ctgccaggga actggcaaaa gaagtagatc
     5401 ttatgatcat tattggtggg aaaaaaagtt ccaacacaaa taaactttat gaaatatgca
     5461 aaaaagtgaa taaaaacacc ctatacattg aaaacttagc tgaaataagg atgcaagata
     5521 tcaaaaagta tggtacaatt ggtatcagtg caggtgcttc tacaccagag tgggtgatta
     5581 ataatattat ggagcatttg caaaatgaga cttaaggagt aactaataca tggaaaactt
     5641 gtcaatgaaa gaaatgatgg aaacagcttc cttcagcgcc ccgaaacgaa atgaggtcat
     5701 tactggaaaa gtcgtaatgg taaaagacga cggtgttgtt gtcaatatcg ggtataaggc
     5761 cgatggtatt cttcctgtgg atgaaatatc aaatcctgaa aatttacctt tagaagaact
     5821 tttcaatgtt agtgatgaaa ttgttgttct tgtgctgaaa agagataatg gcgagggaga
     5881 tgttttattg tctagcaaac gcctacgttc acgcaaggat tgggaaacac ttgaggaaaa
     5941 gtataagaac aaggaatctg ttaatgttaa gatcacagag gttgtaaagg gtggattgtc
     6001 ggcccattat aatgatattc gtgcctttat cccggcaagc catgttgata ttaatttcgt
     6061 tcgcgatctg agtcaatttg tgggagaaga gtgcgaagta gaatttttgg attttgacag
     6121 aagacggaat caagtagttc tttcacgcaa actagtcgta gaaaaagagc gtaaagttga
     6181 actcgatgaa ttctgggata gcctagcaat aggtcaaatc cgagaaggtg ttgttcgtcg
     6241 ctttactagt tatggagcct ttgtagatct tggcgccaca gatggtctta ttcatgtttc
     6301 agaaattcag tggggtaaag taaataaacc ctctgatgtt cttaaagcca atgagacact
     6361 agattttatg gttcttgatt ttagcagaga aaacaataaa atttctctaa gccgcaaagc
     6421 tctatcacca gatccttggg aagtaatcga tgaaatgtat gatgtcggtg aaaattatga
     6481 tggaaaaata gtttcattaa ctgattttgg tgcttttgta gaacttgaac ctggtcttga
     6541 aggactaatt catgtttcgc agatctcaga agatcgtatt gatcagccta aagatgttct
     6601 tagcttaggc gaagaagtga ttgttcgaat ccttgatatc gacaaagaac aaaagagaat
     6661 caaactcagc atgaagcaat aaaaataaaa aaagagcgga ttatcgctct ttttttattg
     6721 gtctacactt ttggtgttgg tatttatttt accaacatct ttttttgcga aagattattt
     6781 tataagcttt gatacacttt taaaacttgc tgtatcactt cgtctttgta aatcaaaaag
     6841 gatagtctct actggaagca caagggctcc caagctttga aggctcttaa gcgcccactg
     6901 actattgtca tcctttcgag atccaaccgc atcataggct atgattactt ctttacctag
     6961 ttctaataga tctctagcag tttgataaac acagacatgg ctttcaattc cactaataat
     7021 aaactgattt cgcccttggg aaaacaagct ttccatgggc ttttttaaat catccattaa
     7081 agagaagcta gttttttcat atagattatc ttctctaatc catttttcta aagaccctac
     7141 tgttgaaccg agtcctctgg gatattgttc gctaaaaaca attggcagac taagaatatc
     7201 tgctccctca agaagaatct gggtgttttt ttcaacttcc tccttatttg aaataacagg
     7261 gagtagtctt tcttgaatat caataacaag aacaagagct tttttttgat ttagtctcat
     7321 atcttcctcc ttttaatcaa ctctatatat atctacccca aatagagaaa ttaatgccgg
     7381 ttggctagaa atactccaag aacaatcata gttgcagcag cccattgttg ggggaggaag
     7441 acctcattaa gaaaaaacac ccctgcaaga accgatgtca gagtggttaa ccctgaaaaa
     7501 gatgaggcaa cagtggggcc tacaagccct attgctgtaa cactcattaa aaaagctaca
     7561 ttgcttgata aaagagatag ataaaccaaa gataataaga accttttatt gacaagagga
     7621 aggagtaaaa aacttttaac ttctccttgg aaagtagcta taagaagact ggctagaaaa
     7681 aaaactaccg ctcccattag catcatggca taggttcgct cataaactgt gaaatccttt
     7741 atttttctct gctttatccc ataaaaacca taggaaaaaa ctcccccaag aagaaaaaga
     7801 taaccaaaaa aggaaaaaga tggctcattt ctttttgtta gaaccataag aataacccct
     7861 aaagtagaag taagtatacc tacaagctgc tcctttctgg gaggttcttg taagaagata
     7921 ctggaaagaa ccatcataac aataggtcct atactcataa taactcctgc ttcagaagca
     7981 gtggtttctc ttattcccaa ggattcaaga gtaaaataaa gaacaggaaa taaagaacct
     8041 aagacaatca aattttgcca accaggtttt tttagcttaa ttggaatacg taaaatgcga
     8101 caaaaaagct caaaaaagat aaaagcaatt ataaatctcc atcccaaaag atgtagaacg
     8161 ctaactgtac ttgtgccagt tttagtaaaa atgaagctaa atccaaatat caaagatgta
     8221 agtagagata ata
//
LOCUS       DASRNA010000148         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_485664,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000148 DASRNA010000000
VERSION     DASRNA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485664"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFD08_04040"
     CDS             complement(<1..591)
                     /locus_tag="VFD08_04040"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZK10222.1"
                     /translation="MSTICAISTSLAEAAIGIVRLSGPASLSIANRIFKGGDLEKKPR
                     YLHYGHIYRADSPIDEVLAVYFRAPYTYTREDMVEINTHGGAMALQRVLALCLEEGAR
                     LANPGEFTQRAFLNGRLDLPQAEAVMQLIQAKTELGYDLALTHLEGDISKKLRQIREI
                     LLSLLASISLAIDYPEEDEEEISYEKLIDGMEKVYKE"
     gene            complement(630..1250)
                     /gene="jag"
                     /locus_tag="VFD08_04045"
     CDS             complement(630..1250)
                     /gene="jag"
                     /locus_tag="VFD08_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HZK10223.1"
                     /translation="MKSVEKTGRTVEEALELALIEMDLSHEDVEYEILENESKGFLGL
                     FGTKEAKILVREKENIHHKAKEFLEDTLKKMNIYAKVEVDFSENGLSGTISGDNMSLV
                     IGRRGKTLDALQYLTNLVINKDREKYVHLILDCEGYREKRKSSLEQLAMRMAEKAKRQ
                     RRDIILNPMNSYERRIVHSALQKDSEVSTRSEGRDPYRKVIIFLNR"
     gene            complement(1250..1951)
                     /locus_tag="VFD08_04050"
     CDS             complement(1250..1951)
                     /locus_tag="VFD08_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZK10224.1"
                     /translation="MTKLFAGILQLIYSLIGNYGISLIIFTILIKLVLFPLAIVQRRG
                     LEANKRMQPKMAELQKKYAKDKNTLNEKMMELYKEENFNPAAGCLPMLIQLPIIFILF
                     RILRDPIPYLGIEALEQSFLWLPNMVGPDLLSNIIPGLTGTLAKLPGILPIVAAFFTY
                     LTSKNAQMQQAPADPNEKPKGPDMGMMTIMFPVMILVFGASYPAGLILYWALSNIIQF
                     IQDLVITRIVASEEL"
     gene            complement(1967..2176)
                     /gene="yidD"
                     /locus_tag="VFD08_04055"
     CDS             complement(1967..2176)
                     /gene="yidD"
                     /locus_tag="VFD08_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZK10225.1"
                     /translation="MKRIFLALIAFYQRRITKHKPKTCRFYPTCSQYSYEAIEKYGAL
                     KGGYLALKRILKCNPFHPGGYDPLK"
     gene            complement(2173..2499)
                     /gene="rnpA"
                     /locus_tag="VFD08_04060"
     CDS             complement(2173..2499)
                     /gene="rnpA"
                     /locus_tag="VFD08_04060"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZK10226.1"
                     /translation="MKTASLTNNKEFKEIYRKANSKVTKYFVIYYRSGKENKSRVGFT
                     ASKKIGNAVVRNLAKRRLKECFRMHEDILEGCYDLVIVARARSSEGNFHQMCKILKKT
                     LEDIQR"
     gene            complement(2527..2661)
                     /gene="rpmH"
                     /locus_tag="VFD08_04065"
     CDS             complement(2527..2661)
                     /gene="rpmH"
                     /locus_tag="VFD08_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZK10227.1"
                     /translation="MKRTFQPKKRQRAKVHGFRKRMKTKSGRRIIKRRRSKGRRNLSA
                     "
     gene            3369..>3968
                     /locus_tag="VFD08_04070"
     CDS             3369..>3968
                     /locus_tag="VFD08_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HZK10228.1"
                     /translation="MQANVAWAKIQNELMLRYDKVSYNTWFRNLEVLNEDEHTVYLLA
                     SSPFNGNVINRRNYSSEFQEIYSDLTGHHKNFAVVTSQDVSHAPQRKLEENPGMRLNP
                     RYIFDEFVVGSSNRFAQAACVAVAEEPGKSYNPLFLYGGSGLGKTHLMQAIGHYILQK
                     DPTKRVMYISSERFTNDFINSIKMGKGEEFRNYYRNNVDV"
BASE COUNT         1122 a          787 c          694 g         1365 t
ORIGIN      
        1 ttctttgtat actttttcca taccatctat aagcttttca tagctaatct cttcttcatc
       61 ctcttctgga taatcaatgg ccaggctaat gcttgctagt aaagaaagaa gtatctcacg
      121 tatctgccgt aatttttttg aaatatcccc ctcaagatgg gtgagagcaa gatcataacc
      181 aagctctgtt tttgcttgga taagctgcat aacagcctct gcctgaggca gatcaaggcg
      241 gccatttaga aaggcccgct gggtaaattc tcccggattt gccagccggg ccccttcttc
      301 taagcaaaga gctaagacac gctgaagcgc catagctccc ccgtgggtgt ttatttctac
      361 catatcttct cttgtatagg tataaggggc cctaaaatag accgctagta cctcatctat
      421 gggactatct gcgcgataaa tatgaccata gtgaagatag cggggttttt tttctaaatc
      481 tcctcccttg aaaatcctat ttgcaataga aagagaagcc ggaccagata ggcggacaat
      541 gccaatggct gcttcagcca gggaggtaga tattgcacaa atcgtactca tggtaatctc
      601 ctttaaaaaa gcccagaatt ctctctgggc tagcggttca aaaagataat tactttgcga
      661 taaggatctc ttccttcgct tcttgtgctc acttcgctgt ccttttgaag ggcggaatgg
      721 acaattcggc gttcataact attcatagga ttaaggatga tatccctgcg ttggcgtttt
      781 gctttttctg ccattctcat agcaagctgc tctaaagaac tttttctttt ttcacggtac
      841 ccctcacaat caaggattag gtgaacatat ttttctcgat ctttattgat tacaagattg
      901 gttaaatact gtagagcatc tagggttttt cctcttctac ctataactag agacatattg
      961 tcgccagata tagtgccaga gaggccattt tcactaaaat caacttcgac ttttgcatat
     1021 atattcattt ttttaagagt atcttctaaa aactctttgg ctttatgatg aatgttttct
     1081 ttttctctta caagaatttt cgcttctttt gttccaaaaa gtccaagaaa acctttgctt
     1141 tcattttcta aaatttcata ttcgacatct tcatgggaaa gatccatttc tataagggcc
     1201 agttctaagg cctcttcaac agttcttccc gttttttcaa cacttttcac tataactcct
     1261 ccgaagcaac tatacgggtg attaccaaat cttggataaa ctggatgata ttacttagtg
     1321 cccagtagag aattagcccc gcaggatagg acgccccgaa taccaaaatc ataacaggaa
     1381 acataatcgt catcattccc atatcaggtc cttttggttt ttcattagga tctgctggag
     1441 cttgctgcat ttgagcattt tttgaagtta gataggtaaa aaacgctgct acaataggca
     1501 aaattccagg aagttttgcg agggtgccag taaggccagg aattatattg cttaaaaggt
     1561 caggtcccac catattgggt agccaaagaa aactttgttc tagggcctct atacctaaat
     1621 aggggattgg atcccttaga attctaaata atataaagat aattgggagc tgtatcagca
     1681 taggtaaaca tccagctgct ggattaaaat tctcctcttt ataaagttcc atcatttttt
     1741 catttaaagt attcttgtct ttagcatatt ttttttgcaa ttctgccatt ttaggctgca
     1801 ttctcttatt ggcttctaag cctcttcttt ggacgatagc aaggggaaag agcactaact
     1861 ttatcaatat agtaaatatg ataaggctaa tcccatagtt tcctataaga gagtatatta
     1921 attgcaaaat gcctgcaaat agtttggtca cgtaagtatc tcctccttat tttaagggat
     1981 catagccacc aggatgaaaa ggattacatt taagaattcg tttaagggcc aaatatcctc
     2041 cctttaatgc accatatttt tctatggctt cataactata ctgggaacag gttggataaa
     2101 aacgacaggt ttttggttta tgcttggtaa tcctgcgttg ataaaaggca attagggcca
     2161 aaaatattct tttcatcttt gtatatcttc aagtgttttt tttagtatct tacacatttg
     2221 gtggaaattc ccttctgatg agcgagctct tgctacaatc accagatcat aacatccttc
     2281 taagatgtct tcatgcatgc gaaagcattc tttaagcctt cgttttgcaa ggtttcggac
     2341 aactgcattg ccaatttttt tactagcggt aaaaccaact cggcttttat tttcttttcc
     2401 tgatcggtaa tagataacaa aatatttggt tactttagag tttgctttac ggtaaatctc
     2461 tttgaattcc ttattattgg tcaaagaagc tgtcttcata gttatcgtct cccggcaggc
     2521 tagccactaa gcactaaggt ttcttcttcc ttttgatctt cttcttttta taattctacg
     2581 accagactta gttttcattc gcttcctaaa cccgtgtact ttggctcgct gacgtttttt
     2641 tggttgaaag gttcttttca ttgatagcac ctccttttct taattagtca agtctcttta
     2701 ttatagccag tgacagcagt aaagtcaaac attcttactt atgaaaaagt ttcaaaacaa
     2761 cccccgtctt tttgaaaaag tttgcttaag ggccttgcgt tctcatttta atcacagatg
     2821 aaacttcatc aacttaattg aacttttaaa agcacgcagt ttcaagctta gatcatccat
     2881 aagtccatat atttgttttt ttcctgttct cattgtcttt tatagtggct tctgactttt
     2941 ttgccttttc tttactcttg tgtacttttt ttctttcttc cacaacatat tgtgtggata
     3001 actttcgttt atttatcatc acttactctt tcaccatatt tataaagcaa aaactgtgga
     3061 taagtccctt cttttgcttg taagctctgt ttttgcatgc tactattatc ctacatgttg
     3121 ataacttagt tctttgcctt tgttttatcc caaacatatc ctttctgtgg ataaagctgt
     3181 ggataagtgc ttagttatcc acgctttccc cttagttcta agtattagcc ctctttataa
     3241 gtcatcaaca tcatgttgat aataccacct tattttattt tatcacaagc tactatttaa
     3301 attggggaaa atatgttaat aaatcaatca accttttgct gtggataagg ttgtggaaag
     3361 gaataaatat gcaagcaaat gtcgcatggg caaaaataca aaatgaacta atgctccgct
     3421 acgataaggt aagttacaat acctggttta gaaatttaga agtcctaaat gaagatgaac
     3481 atactgtgta tttactggca tcaagtccct ttaatggcaa tgttataaat agaagaaatt
     3541 attcctctga attccaagaa atttattcgg acttaacggg acatcataaa aactttgccg
     3601 ttgtcacaag ccaagatgta agccatgctc ctcaaagaaa actagaagaa aatccgggca
     3661 tgcggcttaa tccaagatat attttcgatg aatttgttgt tggttctagt aatcgttttg
     3721 cccaagcagc ctgtgtggct gtagccgaag aacctggtaa atcctataat cccctctttc
     3781 tttatggagg ttcaggcctg ggcaaaaccc atcttatgca ggccataggg cattatatcc
     3841 ttcaaaagga tccaacaaaa agagttatgt atatttcaag cgaacgtttt accaatgact
     3901 ttattaattc aattaaaatg ggtaagggag aagagtttag gaattattat agaaataatg
     3961 tagatgta
//
LOCUS       DASRNA010000149         8269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1010918,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000149 DASRNA010000000
VERSION     DASRNA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010918"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /gene="rny"
                     /locus_tag="VFD08_04075"
     CDS             complement(<1..1505)
                     /gene="rny"
                     /locus_tag="VFD08_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZK10229.1"
                     /translation="MPSMETFLIYIVLAILGIVLGYLLRKSIGERKIRSAENKANDIV
                     LEAKKTAETIKKESLFEAKEEIHNLRSNLEKETRERRSEIQRIERRNQKKEESLDLKQ
                     SEQENRERDLAALDKKMNNIRKQLESQKANQEKELERIASLNREEAKDILLKEVDKTL
                     SREKVLRIRAAEEIIKEESNKTAQKILASTIHRISSDFVADNTVSSVSLPNDEMKGRI
                     IGREGRNIRALEKATGVDLIIDDTPEAVIISCFDPVRREITRMALERLITDGRIHPSR
                     IEEMVNKARDEIEEKMREEGKNAIFELELGRVHPELVKLIGRLQFRTSYGQNVLLHSI
                     EVAKVSGMLAKELGANVRIAKRAGLFHDIGKAVDTEMEGTHIELGMQLLKKYGESKEV
                     IHAMSTHHGDYEPNTIEAVLVTAADAISAARPGARREALNNYIKRLENLEVIANSYKG
                     VDHAYAVQAGREIRIMVIPDKISDDEMLLLSRDVAKRIEDEMDYPGQVKVH"
     gene            complement(1593..2606)
                     /gene="recA"
                     /locus_tag="VFD08_04080"
     CDS             complement(1593..2606)
                     /gene="recA"
                     /locus_tag="VFD08_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZK10230.1"
                     /translation="MSEKNKALDHVLGQIEKQFGKGSIMRLGDEPNRRGIEVIPTGAL
                     SLDIALGIGGIPRGRIIEIYGPESSGKTTVALHMIAEAQKLGGVAAFIDAEHALDPEY
                     AKRIGVDTDGLILSQPDTGEQALEIVDALVRSNAIDVIVVDSVAALVPKAEMTGEMGD
                     SHMGLQARLMSQALRKLTSIVSKSNTSIIFINQLRMKIGVMFGNPETTSGGNALKFYS
                     SLRLDVRRVESIKDRDNIIGNRTRIRVVKNKVAPPFKTALFDIMYGTGISSEGCLLDA
                     AVDAEIVKKAGSWYSYGDERMGQGRENAKTFLIENPETLLEIDHLVREFYEINKTEPQ
                     KTE"
     gene            complement(2641..3747)
                     /locus_tag="VFD08_04085"
     CDS             complement(2641..3747)
                     /locus_tag="VFD08_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HZK10231.1"
                     /translation="MKVGVMTIGDELLQGRILDINKANLGQFFLHRGIEVALSMNPPD
                     EEEALERALRYMIKEVDLIVITGGLGETRDDMTYQITKKVCSDFEEKIVPNHNGAAQG
                     YLLNKDNKSLILVPGPPNENIHMFSTIDDILEKEELFEKLYHLMGIGEYALEQSFQEH
                     FKSESKHLLTYVSIGFVTLRVFSKDKKQVEELGERVEKLYKEYIFYRGQMDLKDKIIS
                     FLTKNKLHLSCAESATAGGVLKALTDISGSSQAIYGGFVVYENTAKMDLLNIDKDFLH
                     KYGSVSQEMSQALAVACQKKTKTEISLAITGYAEHDDPKLSGLCYIHVLTPWGEDVFS
                     RKVPSRRRKSARQSMIIFALASLWKSLAKNLHIE"
     gene            complement(3744..4271)
                     /gene="pgsA"
                     /locus_tag="VFD08_04090"
     CDS             complement(3744..4271)
                     /gene="pgsA"
                     /locus_tag="VFD08_04090"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZK10232.1"
                     /translation="MNLPNKLTILRMLAVPIYFYLALYTDYYLVAFIVFALAALTDFV
                     DGYLARKWDLVTSFGKLMDPLADKFLTISAFIVFSSQQVISPLVLLIVTLREILISVF
                     RAVAASKNIVIAASGYGKAKTVLQMGVIIILHLQPILAFKRGILIELLIWLMVLMTVV
                     SALDYIWKNKEVFSE"
     gene            complement(4255..5553)
                     /gene="rimO"
                     /locus_tag="VFD08_04095"
     CDS             complement(4255..5553)
                     /gene="rimO"
                     /locus_tag="VFD08_04095"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZK10233.1"
                     /translation="MKFYIHELGCAKNTDDVNHMKSLLVDYGHLPLESPEEAETIIIH
                     TCGFIEEAVSQSIEAILDLADFKEFNCNQLIVSGCLAQRYQESLLKELPEVDLFLGTS
                     YSHRIQEFINKKGAYFDSLDFNLSTFKHLSANSSYAYVKIAEGCDNFCSYCIIPSLRG
                     PMRSKSIDQIVIEAENLASQGIKEIILIAQDLSRYGEDRGKRQLLELLASLNAIEGIY
                     WIRLQYVYPDLLDEAFFHKLAELSKVVPYLDMPIQHASDSVLRKMNRHTSQEDLRKII
                     TMARQIIPNIVLRTTVMVGFPGESEEDFELLLDFVKEISFDKLGAFKYSDEEDAASYL
                     LPNKISDQIKEERYQRLMNLQKDISEKKLDLCVGKTMDVLVDEIYEDSWIGRTKGDSP
                     DVDGIVYVKGKDVSLGDILKVKIVSNSEYDLIGEVNESTK"
     gene            complement(5534..6556)
                     /gene="mnmH"
                     /locus_tag="VFD08_04100"
     CDS             complement(5534..6556)
                     /gene="mnmH"
                     /locus_tag="VFD08_04100"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HZK10234.1"
                     /translation="MELTVEEALLKNYLFIDMRSPKEYGHSHIPFATSLPLLDDVSRE
                     IIGSIYKKEGREKAIDKGLEHVLPRVENLLIQINNATINREVILYCARGGMRSASVYK
                     LFENNPSVHLLIGGYKSYRRFVLDDMPRQIEKRKIILFQGYTGVGKTNLLHQLKSDYD
                     ILDLESLANHRGSVFGDIGLKDQPSQKQFESDIYHGIRESGKMILVEAESSKIGKLHL
                     PKDLHVKMLKAPIFYLEASLKSRVDLLHDEYGADLVDNIDELIRDTWKLKRQLGYKAC
                     EKLVNLLSTGEMKKAIELLLTLYYDPLYKHSIKKNTYRVENKFESDAPDILEQIGRHL
                     DEILYS"
     gene            complement(6547..>8269)
                     /locus_tag="VFD08_04105"
     CDS             complement(6547..>8269)
                     /locus_tag="VFD08_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZK10235.1"
                     /translation="NKAHIKKTQARVNQAKEENESFLLGSTRKPESLKKARANEALPE
                     FNLSDDYIQVNLEEENEPIISLPQEENQEEEKIEKVLEKPTPSETEAFNKSLEELNKT
                     KTVSYLKPPISLLHTRTKNSTKKTVKKKEMLELARKLEEIMNTFSVNAQVLEVVKGPI
                     VNRFEIQLEAGTKISKITGLADDIALHMAVSQVRVAPITGKSTIGIEIPNKEKDPVSL
                     YEILRSKEYKNVNSKLAVALGKDITGKAVVADLAQMPHLLIAGSTGSGKSVCINTIIA
                     GLLFRSSPDEVKLLMIDPKVVELSNYNGIPHLILPVVTEPKKAALALGWAVNEMTRRY
                     EAFAENKVKDIQSYNKKLKDEKMPRIVVIIDELADLMMVAQAQVEDSIARLSQMARAA
                     GIHLIVATQRPSAEVITGQIKTNIPSRISFAVSSAVDSRIILDMSGAEKLLGKGDMLY
                     FPFGSNRPKRVQGCFLSEEEIGSIVDFIKNQNIQTDYKEDIVKKTSPRIESQFDDEYL
                     EDAIRLVIEMKSASASMLQRRFRIGYNRAGRLIDNMAEMGIVGPPQASKPRDVLVSDF
                     EEVEPWN"
BASE COUNT         2382 a         1695 c         1381 g         2811 t
ORIGIN      
        1 atatgaactt taacttgacc tggatagtcc atttcatctt caatgcgttt ggccacgtct
       61 cttgatagaa gaagcatctc atcatcactg atcttatcag gaattaccat aattcggatt
      121 tctctgcctg cttgaacagc ataggcgtgg tctacgccct tataggaatt tgcgataact
      181 tctaagtttt caagtcgttt gatatagttg tttagagctt cacgtcttgc tcctggcctg
      241 gcagcgctaa tggcgtctgc tgctgtaaca agaacagctt ctattgtgtt aggctcatag
      301 tctccgtgat gtgtactcat ggcgtggatt acttcttttg attcaccata ttttttaaga
      361 agttgcatac ctagttcaat atgcgtaccc tccatttccg tatcaacggc tttgcctata
      421 tcatggaata gcccagctct tttagcgatt cggacatttg cccctagttc cttagccaac
      481 atacccgaaa ctttggctac ttcaatgcta tgaagaagaa cattttggcc ataactagtt
      541 ctaaattgta agcggccaat tagcttaaca agttcaggat gaactcttcc gagttctagt
      601 tcgaagatgg cgtttttacc ttcttctctc atcttttctt caatttcatc tcgtgccttg
      661 ttaaccattt cttcaattcg gcttggatga attcgtccat cagtaataag tctttcaagg
      721 gccatgcgcg taatttcacg acgcacggga tcaaaacacg aaataataac tgcctctggt
      781 gtatcatcaa ttattaaatc tacaccagtt gctttttcaa gggcacgtat attgcgtccc
      841 tctctaccaa taattcgacc cttcatctca tcatttggca atgagacaga actgacagta
      901 ttgtctgcaa caaagtcaga agaaatgcga tgtatggtgc ttgctagtat tttttgcgct
      961 gttttatttg attcttcttt aattatttct tcagctgcac ggatacgtaa taccttttca
     1021 cgagaaagtg ttttatcaac ttcttttaat agaatatctt tagcttcttc tctgtttaat
     1081 gaggctatac gctctagttc tttttcttga ttggcttttt gtgattcaag ctgttttctt
     1141 atattattca tttttttatc aagggcagct aaatcacgct cacgattttc ttgttctgac
     1201 tgcttaagat caagactctc ttcttttttt tgattccttc gctcgatgcg ttgaatttcg
     1261 cttcgacgtt ctcttgtttc tttttccaga ttagatcgta gattgtggat ttcttctttg
     1321 gcttcgaaaa gcgattcttt ttttatagtt tctgccgtct tttttgcttc aagtactata
     1381 tcattggctt tattctcagc agatctaatt tttctttcac caatactttt tctgagtaga
     1441 tatccaagta caataccaag tattgctaat acgatataaa taaggaatgt ttccatacta
     1501 ggaatataaa tcacctcctt tatttatcta acgtacatat tataacatat attcgttatt
     1561 catgcttgga ctctaaggca aaaagacgga aattattccg tcttttgggg ttcagtttta
     1621 ttgatctcat aaaattccct aaccaaatga tcaatttcta gtagtgtttc agggttttct
     1681 ataagaaaag ttttagcatt ctctcttcct tggcccatac gctcatcacc ataggaatac
     1741 caagaaccag ctttttttac aatttcagca tcaacggctg catctaagag gcagccctca
     1801 ctcgaaatcc ccgttccata cataatatca aataaggctg ttttaaaggg cggggcgact
     1861 ttgtttttaa ctacacgtat acgagtacgg tttcctatta tattgtctct atcttttatc
     1921 gattcgaccc ttcgaacatc aagacgcaag gaagaataaa atttaagtgc attaccacct
     1981 gaagtagttt caggattccc aaacataaca ccaatcttca tacgaagctg gttgataaag
     2041 ataatacttg tattgctttt actgacaata ctagtgagtt tacgaagggc ctggctcata
     2101 agccttgctt gaaggcccat atggctatct cccatttcac cagtcatttc tgccttggga
     2161 accaaagcag ccacagagtc cacaacaata acatcaatgg cattactacg aacaagcgca
     2221 tcaacaatct ctaaggcttg ttctcctgta tcaggttgag acaggattaa accatctgta
     2281 tcaacaccaa ttctttttgc gtattctgga tctagggcat gttcagcatc aataaaagct
     2341 gccactcctc ctagtttttg tgcttctgca atcatatgaa gagccacggt agttttccca
     2401 ctggattcag gaccgtaaat ttcaatgatt cttcccctag gtattccacc tatccccaat
     2461 gcaatatcta aggacaaggc tcctgtagga ataacttcta tccctcttct attaggttca
     2521 tcgccaaggc gcataattga acctttacca aattgttttt cgatttggcc aaggacatga
     2581 tctaatgctt tatttttttc agacatatta cctcccgaac ataagttcta tactaccatt
     2641 ctattctata tgcaagtttt ttgcaaggct tttccaaaga gaagctagag caaagataat
     2701 catggactgt ctagctgatt ttcgacgtcg gctcggtact ttgcgtgaga acacatcttc
     2761 accccagggg gttaaaacat gtatataaca caaaccactt agtttaggat catcatgttc
     2821 agcatagcca gtaatagcaa gtgatatttc tgttttcgtc tttttttgac atgcgacagc
     2881 tagggcttga gacatttctt gacttacact tccatactta tgcaaaaagt ctttgtcgat
     2941 atttaaaagg tccatcttgg ctgtgttttc atagactaca aagccaccat aaattgcttg
     3001 actggaacca ctaatgtcag ttagagcttt tagaacccct ccagcagtgg cggattcagc
     3061 gcaagaaaga tggagcttgt tctttgtaag aaaggagata attttatcct ttaagtccat
     3121 ttgaccacga taaaatatat attctttata aagcttctct acacgttctc ccagctcttc
     3181 gacctgtttt ttatctttag aaaatacccg taaagtaaca aatcctattg aaacataggt
     3241 cagtaaatgc tttgattcag acttgaagtg ttcttgaaaa ctttgttcta aagcatactc
     3301 ccctattccc attagatgat aaagtttttc gaaaagctct tccttttcca aaatatcatc
     3361 gatggtagaa aacatgtgta tattttcatt aggaggccct ggtacaagga taagagattt
     3421 attgtcttta ttcaagagat atccctgcgc tgcaccatta tggtttggga caattttttc
     3481 ctcaaaatca ctgcaaactt tttttgtgat ttgataggtc atatcatcac gagtttctcc
     3541 taggccgcct gtgattacta taagatcaac ttcttttatc atatagcgaa gagctctttc
     3601 taaagcttct tcttcatcgg ggggattcat tgaaagggca acctcaatgc ctcggtgtaa
     3661 aaaaaactgc cctaaattgg ccttatttat atctaaaata cgaccttgaa gaagttcatc
     3721 acctatggtc attactccga ctttcattct gagaacacct ctttattttt ccaaatataa
     3781 tccaaagctg aaactaccgt cataagaacc attaaccaga ttaaaagttc aataagaatt
     3841 cctcttttaa aagctaaaat aggctgcaaa tgtaaaatga taataacacc catttgtaaa
     3901 acagtttttg cttttccata tccactggca gcaataacaa tattttttga agcagccacg
     3961 gccctaaaaa cagaaataag tatttctctt aaggtaacaa taagcaaaac aagaggggaa
     4021 ataacctgtt gagaagaaaa gactataaag gcagatatag taaggaattt atcagctagg
     4081 gggtccatta actttccaaa ggaagtaacc aggtcccact tcctagccag atacccatca
     4141 acaaagtctg ttaaggctgc taatgcaaag actataaagg caactaagta ataatccgtg
     4201 taaagggcca ggtagaaata aattggaact gctagcatac gaagtatggt taatttattt
     4261 ggtagattca tttacttctc ctatcaaatc atattcagaa ttgcttacaa tcttgacttt
     4321 aagaatatct ccaagactta catcttttcc ttttacatag acaatcccat caacatctgg
     4381 tgaatcacct ttggtgcgtc caatccaaga atcttcatag atttcatcga caagtacatc
     4441 catggttttt cccacacaca aatctagctt tttttcagat atatcttttt gcaaattcat
     4501 aagcctttga tagcgctctt ctttgatttg atcagatatt ttattaggca gaaggtagct
     4561 agccgcgtct tcttcatcag aatatttaaa ggctcccaat ttatcaaagg atatttcttt
     4621 tacaaaatca agaaggagct caaaatcttc ctcactttcc ccaggaaaac caaccattac
     4681 cgttgtccga agaacaatat taggaataat ttgcctagcc atagttataa tttttctaag
     4741 atcttcttgg ctagtatggc ggttcatttt tctaagaaca gaatcactag catgttggat
     4801 gggcatatct aagtagggaa caaccttaga aagctctgct aatttatgga agaaagcttc
     4861 atctaaaaga tcaggataaa catactgaag acgaatccaa taaatgcctt caatagcatt
     4921 taaagatgct aaaagttcta gtagttggcg ctttccccta tcttcaccat agcgacttaa
     4981 gtcttgagct attaaaataa tttcttttat cccttgactt gctaaatttt cagcttctat
     5041 tacaatttga tcaatgctct tacttctcat agggccccgt aaagagggaa taatgcaata
     5101 actacaaaag ttatcacagc cctcagcaat ttttacataa gcataggaac tattagcgct
     5161 cagatgctta aaggtgctta gattaaaatc cagcgaatca aaataagctc cttttttatt
     5221 gataaactct tggattctat gactgtagga ggttcccaaa aaaagatcta cttctggaag
     5281 ttcctttaaa agggattctt gataacgctg ggctaagcag cctgaaacaa taagttgatt
     5341 acagttgaat tctttgaaat cagctaaatc taaaattgct tctatgcttt gacttacggc
     5401 ttcttcaata aatccacagg tatggataat gatggtttcc gcctcctctg gtgattccag
     5461 gggtaaatgc ccataatcta ccagcaggct tttcatatga tttacatcat cagtgttttt
     5521 tgcacaacca agttcatgaa tataaaattt catctaaatg tcttcctatt tgttctaaaa
     5581 tatcaggggc atcactttca aacttatttt ctaccctata agtgtttttc ttaatactat
     5641 gtttatacaa gggatcatag tacaaggtaa gcaaaagttc tattgctttt ttcatttcac
     5701 ctgtactaag taaattaaca agtttctcac atgccttgta ccctaattga cgctttaatt
     5761 tccaagtgtc acggattaat tcatctatat tgtctacaag gtctgcacca tattcatcat
     5821 gcaataagtc aacgcggctt ttaagacttg cttccaagta aaatatcggg gccttaagca
     5881 tcttaacgtg aagatcttta ggtaaatgta atttaccgat ttttgatgat tcagcttcaa
     5941 ctaaaatcat tttaccagat tcacgtatcc catgataaat atcactttca aattgctttt
     6001 gcgaaggctg gtctttaaga ccaatatcac caaaaacaga accccgatgg tttgctagag
     6061 attcaaggtc caatatatcg tagtctgatt ttaattgatg gagtagattg gtctttccta
     6121 ctccagtata tccttgaaaa agaataattt ttcttttttc tatttgtcgg ggcatatcat
     6181 ctaagacaaa acgtctataa cttttatacc caccaattag gagatgaaca gaaggattgt
     6241 tctcaaagag tttatagaca gaagcagaac gcataccacc tctagcacag taaaggatga
     6301 cttctctatt aatagtggca ttatttattt gaatcaatag gttttctacc cgaggaagta
     6361 catgctcaag ccctttatct attgcctttt ctcgtccttc ttttttatat atggatccta
     6421 taatttccct ggatacatca tcaagtaggg gtaaagaggt ggcaaaagga atatgggagt
     6481 gaccatattc ctttggacta cgcatatcaa taaataaata gttttttaga agagcttctt
     6541 ctactgttaa ttccatggtt ccacttcctc aaagtcacta accaatacat ctcttggttt
     6601 actagcttgg gggggaccga caatacccat ttcagccata ttatcaataa gtcttccagc
     6661 tctattataa ccgattcgaa aacgacgttg aagcatactg gctgatgcac ttttcatttc
     6721 aataacaagt cgaatagcat cttcaagata ctcatcatca aactgacttt ctatcctagg
     6781 ggaagttttt ttaacaatgt cttccttgta gtctgtttga atgttttggt ttttaataaa
     6841 atcaacaata cttcctattt cttcttcgga aagaaaacag ccctgtactc tttttggcct
     6901 gttgctacca aagggaaaat aaagcatatc acctttaccc agtagttttt ctgccccact
     6961 catatctaga ataattcttg aatcaacagc agagcttacg gcaaatgaaa tacgtgaagg
     7021 gatattggtt tttatttggc ctgtaattac ttcagccgag ggtctttgcg tagctacaat
     7081 aagatgaatg ccagcagctc tggccatttg cgatagacgg gcaatactat cttctacttg
     7141 ggcttgagca accatcataa gatctgcaag ctcatcaata ataacaacaa tacgtggcat
     7201 tttttcgtct ttaagcttct tattatagct ttgaatatct ttgaccttat tttcagcaaa
     7261 ggcttcatag cgccgagtca tttcattaac tgcccaacct agcgccaggg ctgctttttt
     7321 gggttctgtt accacaggca aaattagatg tggaatccca ttgtaattac ttagctccac
     7381 gactttagga tcaatcatta aaagctttac ttcatcagga ctggacctga aaagaaggcc
     7441 agcaataata gtatttatac atacactttt acctgaacct gtactccctg caataagaag
     7501 atggggcatt tgcgccaggt ctgctacaac agcttttcct gtaatatctt ttccaagggc
     7561 cacagccagc ttggaattaa cgtttttata ttctttgctt cttaatattt cataaagaga
     7621 aactggatct ttttctttat taggtatttc tatgccaatg gtactttttc ctgtaatagg
     7681 ggccactcgg acctgggaaa cagccatatg tagagcaata tcatctgcaa ggccagttat
     7741 ttttgaaatt tttgttcccg cctctagctg gatttcaaaa cgattgacga tgggaccctt
     7801 aactacttca agaacttgag cattaacaga aaaagtattc attatttctt ctagttttct
     7861 ggctagttcc agcatttctt tcttttttac cgttttcttt gtggagtttt tagttcttgt
     7921 atgaagcaag gaaatcggtg gttttagata agatacagtt ttagttttat ttagttcttc
     7981 caggcttttg ttaaaggctt cagtctcaga aggtgttggc ttttcaagaa ctttttcgat
     8041 tttttcttcc tcttgatttt cttcttgagg tagtgagatg attggttcgt tttcttcttc
     8101 taggttcacc tggatataat catcacttag attaaactct ggcaatgctt cattagccct
     8161 agcctttttc aaactctcgg gttttctagt agaaccaagt aaaaaagatt cgttttcttc
     8221 ttttgcttgg ttaaccctag cctgtgtctt ttttatatgg gccttattt
//
LOCUS       DASRNA010000150         5752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1091927,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000150 DASRNA010000000
VERSION     DASRNA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091927"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1103)
                     /locus_tag="VFD08_04110"
     CDS             complement(261..1103)
                     /locus_tag="VFD08_04110"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HZK10236.1"
                     /translation="MMKFGVIGTGTMGAGIVQILAQEGHEVIMMARRESSIARGFATM
                     EKGLARNVKRERMTQEEMDATIALVKGTNNLEDLKDVDFIIEAASERLDAKKELFAKL
                     DELLGEDVILATNTSSLSITEIASATNRPDKVIGMHFFNPVPMMKLVEVICGVATSAE
                     VEAKVNALAEELGKTPVRVEEAPGFVVNRILIPMINEGIGIYADGVASAEDVDNAMKL
                     GANHPMGPLALGDLIGLDVCLSIMDVLYSEFQDPKYRAHPLLRKMVRANLLGRKTKKG
                     FFDY"
     gene            complement(1118..1891)
                     /locus_tag="VFD08_04115"
     CDS             complement(1118..1891)
                     /locus_tag="VFD08_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZK10237.1"
                     /translation="MDYLRLKWHVKDRVGLLTLNNPKTLNALDSQTLRELEAWVDGPA
                     RDKDLRAIVITGEGKAFVAGANIKEMLSLDVEEAKVFALLGSEVFRKIEKLPLPVIAS
                     VNGFALGGGCELALSCDIRIGSKKAKFGQPEVGLGITPGFSGTVRLPKVVGGAKAKEL
                     IYTGKIIGAEEALSIGLLNQLVETEELMEKTMEMANQIAANSASAVSKTKEAINRGLE
                     TDSDSAISIENDLFALCFAHQDQKEGMQAFVDKKKADFK"
     gene            complement(2110..2322)
                     /locus_tag="VFD08_04120"
     CDS             complement(2110..2322)
                     /locus_tag="VFD08_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10238.1"
                     /translation="MMFSKKPENDLKSRRQKAKEQNEKLSRLRRETQLEKGDMLALIL
                     GAFLALLPYVLIILLAYYFISMLIFG"
     gene            complement(2319..3476)
                     /locus_tag="VFD08_04125"
     CDS             complement(2319..3476)
                     /locus_tag="VFD08_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZK10239.1"
                     /translation="MSKVLVETRNLKQYFPTGKKRDEKDKIKRVRQQALDLYNEKFNK
                     RIRIKELVKMYNERQDLGKELYDLLAEHERLTELDGQPLSVKANDGISIKIHEGETVG
                     LVGESGCGKSTLGRAILKLYKLTDGQVFYDGKDITNYTDKQMIPLRREMQIIFQDPYS
                     SLNPKMTVGQIIGEALHEHKMFKKGSDKLEEYVKEIMNTCGLDDYMIYRYPHEFSGGQ
                     RQRIGIARALALKPKFIVCDEAVSALDVSIQSQIINLLNDLQKEFGLSYLFISHDLSV
                     VKHISDTIGVMYLGDIVEYAKKKKLYENPLHPYTKALLSAIPETDLERVRNKQRILLQ
                     GDIPSNIIVPPGCKFHTRCPIAQDICKVEIPEYREAEEDHFVACHFSGAKL"
     gene            complement(3469..4461)
                     /locus_tag="VFD08_04130"
     CDS             complement(3469..4461)
                     /locus_tag="VFD08_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10240.1"
                     /translation="MNKNLLEIKNLHTYFFTNKGTVRAVQGVDLTIEPGKTLGIVGES
                     GSGKSVTAFSILNLLDAPGEIVEGEILFAGRNLVGARESELRDIRGNDISMIFQEPMT
                     SLNPVHRVGKQLSESLILHQKMSKKEAWTSSIELLREVQIPNPENVIYNYPFQLSGGM
                     RQRVMIAMALACQPLLLIADEPTTALDVTIQAQIFKLMNDLKTKLNTAIMFITHDLGA
                     IAELADDVAVMYTGEIVERAPVDTIFDRDSEYSHPYTEGLLKSIPMLDVEVEYLEQIE
                     GSVPHPLKLPLGCRFSTRCKYVTEKCINEKPGLVELESGHSVRCFYPKTRGRHE"
     gene            complement(4462..5478)
                     /locus_tag="VFD08_04135"
     CDS             complement(4462..5478)
                     /locus_tag="VFD08_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002693257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10241.1"
                     /translation="MANLEDNTGFSAADEVILSPTQQIIIKFKHNRLAMVGFWLFLFI
                     VSLIVVTHFYTKLTGYNFAKTDASLKYLPPSLKYPFGTDQYGRDTFIRVLEGGWISLQ
                     VGVLSTVMAIVIGVFMGSVAGYFGGKIDYVIMRIVEIIYSFPFLALAYTISAIFRDKP
                     AKFRLFVIIAILGFIRWTGVARLVRGQILSLKEQDFILATKALGIKTRNQIIRHLIPN
                     VLSTVVVSATLTFAAAILGEAFLSFLNLSVTEPIPTWGALLSRASQNSTNLRNYWWIW
                     VFPGTMLFAFIMSINLIGEGLREAVDPKAQYSTKAERKETRRRRKLQKSLAKAERNAN
                     KGAV"
     gene            complement(5471..>5752)
                     /locus_tag="VFD08_04140"
     CDS             complement(5471..>5752)
                     /locus_tag="VFD08_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK10242.1"
                     /translation="IYRHAFQNALIPVVTLIGMYIPSLFGGSIVVERIFAYPGMGFLL
                     GNAYSYKDRAVLQTALLFFGLLTLLGNIFIDVGYMIVDPRIREGKGFDG"
BASE COUNT         1698 a         1232 c          961 g         1861 t
ORIGIN      
        1 ctatagataa gggcttttgt gcaagaagct tcaagctaat aaaagcttct ttttctttct
       61 ttcattcttt tattttgagt cagtttggga tctactaagc cccttattta tacctactaa
      121 ctttggatat aaggccctta tcaccttgac aaatgcaaag aatatttcac ctgtgcttgc
      181 ccgagatagt ataagtcatt agaaattggg gaaaaagacc cttagaagat agttatcatc
      241 taagggtcct tatttagtgc ttagtagtca aaaaagcctt ttttagtttt tcttcctaaa
      301 agattcgcgc gaaccatctt tcttagaaga ggatgggctc tgtattttgg atcttgaaac
      361 tcactataga gtacatccat aatactaagg cagacatcta gaccaataag atctcccagg
      421 gctaagggac ccatggggtg attggcacca agcttcattg cattatcaac atcttctgca
      481 ctggccacgc catcggcata aattcctata ccttcgttta tcataggaat aagaatacga
      541 ttaacaacaa atcctggagc ttcttctact cggactggtg tttttccaag ttcttctgct
      601 agggcgttaa cctttgcttc tacctcagca gatgtagcta caccacagat tacttcaact
      661 agtttcatca taggtacggg gttgaaaaag tgcataccta ttactttatc aggtcgattt
      721 gtcgcagaag ctatctcagt aatagaaagg gatgatgtat tggttgcaag tataacatct
      781 tctcctagga gttcatcaag cttagcaaaa agctcttttt ttgcatccaa tctctcagaa
      841 gcagcttcaa taataaagtc tacatcctta agatcttcta gattgtttgt ccccttaaca
      901 agggcgatgg tagcatccat ttcttcttga gtcatacgtt ctctttttac attgcgcgct
      961 aggccttttt ccattgtggc aaagcctctg gcaatcgaac tttctcttct tgccatcata
     1021 ataacttcat ggccctcttg agcaagaatt tgtacaatac ctgctcccat agttcctgtt
     1081 ccaattactc caaatttcat aatatctcct ctacctccta tttaaagtca gccttttttt
     1141 tatctacaaa ggcttgcatt ccctcttttt ggtcctggtg agcaaaacaa agtgcaaata
     1201 aatcattttc tattgaaatt gcactatcac tatctgtttc caggcctcgg tttatcgctt
     1261 cttttgtttt acttaccgct gaagctgaat ttgctgctat ttggtttgcc atttccatgg
     1321 tcttttccat cagttcttct gtttcgacta gttgattaag aagacctatg ctaagagcct
     1381 cctctgcgcc aatgattttt cccgtataga taagttcttt tgctttggcc ccacctacaa
     1441 cttttggaag acgtaccgtt cctgaaaaac caggagtaat gccaaggccg acttccggct
     1501 gaccaaattt agcttttttt gatccaatac gaatatcaca agaaagtgcc agttcgcatc
     1561 caccccctag ggcaaagcca ttaacggatg ctataacagg aaggggaagc ttttcaattt
     1621 tcctaaaaac ctcagaacca agtagagcaa aaactttggc ttcctctaca tccaaagaca
     1681 acatttcttt aatattagct ccagctacaa aggcttttcc ttcgcctgtt attacaatag
     1741 ctcttaagtc cttgtcccta gctggtccat ctacccaggc ctcaagttcc cttaaagttt
     1801 ggctatccag cgcgttaaga gttttagggt tgtttaatgt aagaagacct accctatcct
     1861 taacatgcca cttaagtcgt aagtaatcca tccaaacctc ctcaaattga cagttcttta
     1921 actaagttaa ttgtaacaaa taaatatcca aattgcaatt acaattaaat agcgactccc
     1981 aaacttggaa aagtcgttat ttaataccaa ggtctaacta agtaaaacaa taagcagaat
     2041 aaaaaccacc ctctcatacc atacttcctt cctagctata tttagaaagg acttgtcctg
     2101 tctagcattc tatccaaaaa tcagcatgga tataaaatag taagctaaaa gaattatcag
     2161 tacatagggc aataaggcaa gaaaagcacc taatataaga gcaagcatat cccctttttc
     2221 taactgcgtt tctcttctta acctactaag tttttcgttt tgttcttttg ccttctgcct
     2281 tctagatttt agatcatttt ctggtttttt ggaaaacatc atagtttagc accactaaag
     2341 tggcaggcta caaaatgatc ttcttcagcc tccctatatt caggaatttc aaccttacag
     2401 atgtcttgtg ctattggaca ccttgtatgg aacttacatc ctggaggaac aataatatta
     2461 gagggaatgt cgccttgtag caagatacgt tgtttgtttc tcactctttc taaatctgtc
     2521 tcaggaatag ctgaaagaag cgcttttgta tatggatgca aggggttttc atataatttc
     2581 tttttctttg catactcaac tatatcaccc aagtacatca caccaattgt gtcacttata
     2641 tgcttaacaa cagataagtc atgtgaaata aacaaatacg ataaaccaaa ctctttttgc
     2701 aaatcattta acaagttaat aatctgagat tgtatggata catctagggc tgaaacagcc
     2761 tcatcacata caataaactt tggtttaagt gctaaggcac gcgcaatccc gattctttga
     2821 cgttgaccac ctgaaaactc atggggatat cgatagatca tatagtcatc gagtccacag
     2881 gtattcataa tttcttttac atattcctca agcttatcag aacccttctt aaacatttta
     2941 tgctcatgca aggcttctcc aataatctgg ccaacagtca tcttaggatt taaagaggaa
     3001 taggggtctt ggaaaataat ttgcatttcg cgtctaaggg ggatcatttg cttgtcagta
     3061 tagttggtta tatcttttcc atcataaaaa acttggccat cagtcagctt atagagttta
     3121 aggattgctc tacctagggt agacttacca catccagact caccaaccaa tcccacagtc
     3181 tccccttcat gaattttaat actaataccg tcattagctt ttacagatag tggttgtcca
     3241 tcaagctctg taagtctttc gtgttcagct agtaaatcat ataattcttt tccaaggtct
     3301 tggcgctcat tatacatttt tactaattcc ttgatacgaa ttcttttgtt aaacttttca
     3361 ttatataggt caagagcctg ctgtcttact ctttttatct tatctttttc atctcttttt
     3421 ttgcctgtag ggaaatactg cttaagattt cttgtttcta ctaaaacttt actcatgacg
     3481 cccccttgtt ttaggataaa aacacctaac tgaatgtcca ctctctagct caacaagtcc
     3541 aggcttctca ttaatgcatt tttcggtaac atacttacat cgggttgaga acctgcatcc
     3601 tagaggtagt ttaagtggat ggggtacact tccttcaatt tgttctaaat attctacttc
     3661 tacatccagc attggaatcg atttaagtag cccttccgta tagggatgag aatattcaga
     3721 atctctatca aagatggtgt ctacaggagc tctttctaca atttctcctg tgtacattac
     3781 tgcaacatca tcagctagtt cagcaatcgc ccccaggtcg tgggtgataa acataatagc
     3841 tgtattgagt tttgtcttta aatcattcat caatttaaat atctgggctt gaatggtaac
     3901 atccagggcc gttgttggct catctgcaat aagaagaaga ggttgacaag caagagccat
     3961 tgcaatcata acacgctgcc tcataccccc agaaagttgg aaaggataat tgtaaattac
     4021 attttctgga tttggtattt gcacttctct taaaagctct atcgaggatg tccatgcttc
     4081 ttttttagac attttttggt gaagaatcaa agattcactg agttgtttac caactctatg
     4141 aactggatta agcgaagtca taggctcctg gaagatcata gaaatatcgt tacccctgat
     4201 atctcttagt tcagactcac gagctcctac taggtttcta cctgcaaaaa ggatttcccc
     4261 ttcgacaatt tctccgggtg catctaacaa gttaagaata ctaaaggccg taaccgattt
     4321 gcctgatcca gattcaccaa cgataccaag tgttttgcct ggttcaatag ttaaatcaac
     4381 gccttgtacg gccctaactg tacccttatt ggtaaaaaag taagtgtgca aatttttaat
     4441 ttcaagtaaa tttttattca tttacacagc tcctttatta gcatttcgct ctgctttggc
     4501 cagacttttt tgtagttttc gtcttcttct agtttctttt ctttcggcct tggtagaata
     4561 ctgagcttta gggtcaacag cttctcgaag accttctcca attaagttaa tgctcataat
     4621 aaaggcaaat aacatagtcc ctgggaaaac ccaaatccac caatagttcc ttagattcgt
     4681 actgttttga gaagccctag atagtagcgc accccaagta ggaatcggct ctgttaccga
     4741 taagttcaag aaggatagga atgcttcacc taaaatcgct gccgcaaagg taagtgtcgc
     4801 agaaaccaca actgttgaaa gcacattagg aattaagtgt cttatgattt ggtttcttgt
     4861 ctttattcca agggcttttg tcgctaaaat aaagtcttgt tccttcaagg ataaaatctg
     4921 tcctcttacc agtcttgcaa caccagtcca ccttataaag cccagtattg caataattac
     4981 aaataaccta aatttagctg gcttatctct aaatatggca gatatggtat aggcaagtgc
     5041 aaggaaggga aaggagtaga taatttctac tattctcata ataacgtaat caattttccc
     5101 gccaaagtat cctgccacag agcccataaa aacacctata actatggcca taacggttga
     5161 taaaacacct acctgcaagg aaatccatcc accctcaaga accctgataa aggtgtctcg
     5221 accatactgg tccgtaccaa agggatattt aagacttggt ggtagatatt tgagtgatgc
     5281 atccgtttta gcaaaattat atcccgtaag ttttgtgtaa aagtgtgtca ctacaataag
     5341 ggatacaata aaaagaaata accaaaatcc aaccattgct agtctattgt gtttgaattt
     5401 aataataatt tgttgagtcg gtgataaaat gacttcatca gctgcgctaa aaccagtgtt
     5461 atcttctaaa ttagccatca aatcccttcc cttctctgat tcttgggtca acaatcatat
     5521 atccaacatc aataaatata tttcccaata gggttagtag tccaaaaaac agtagtgcag
     5581 tttgtaatac tgctctatct ttatatgagt aagcattgcc taataaaaat cccatgcctg
     5641 gataggcaaa gattctttca acaacaatcg agccaccgaa taaactaggt atatacatac
     5701 ctataagtgt aacaacaggt ataagagcat tttggaaagc atgcctatat at
//
LOCUS       DASRNA010000151         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_639966,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000151 DASRNA010000000
VERSION     DASRNA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639966"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VFD08_04145"
     CDS             complement(<1..113)
                     /locus_tag="VFD08_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HZK10243.1"
                     /translation="MATRGRKPKPTALKVLEGNPGKRPLNKNEPQPERKAPR"
     gene            complement(246..452)
                     /locus_tag="VFD08_04150"
     CDS             complement(246..452)
                     /locus_tag="VFD08_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HZK10244.1"
                     /translation="MDKFFTQKNCDRCGKDLSNGRIMSMFNKDCICMDCKGKEREDPE
                     YKKAADAEHEAVKRGNLNFPGIRK"
     gene            complement(463..651)
                     /locus_tag="VFD08_04155"
     CDS             complement(463..651)
                     /locus_tag="VFD08_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10245.1"
                     /translation="MTKPRCKLTGEDGNIFNLLGIARRTLKRAGLGAQAQEMTERVTQ
                     SGSYHEALAVIMDYVEVE"
     gene            complement(652..1359)
                     /locus_tag="VFD08_04160"
     CDS             complement(652..1359)
                     /locus_tag="VFD08_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10246.1"
                     /translation="MSRKELVNLLSQRLEVDSVYLGAPSFAYQVGDYTVDRHGSILDA
                     QGQAVEPEKLLESGSKEEMGLETLNVQIPLEGYDGKGLRNLLHLIYNNQPLIKKALDL
                     KSDLVSEEIIAALQQERMFTLEHFKEAMKGVSCPFIDFDYDDEIIVFRLDTYEKNPEK
                     VQAATQLLKLANINARRRKRNLAAKVRSTDNEKFSFRTWLLNLGMIGDEYKVARKVLL
                     KNLSGNSAFRNPLGEEA"
     gene            complement(1425..2660)
                     /locus_tag="VFD08_04165"
     CDS             complement(1425..2660)
                     /locus_tag="VFD08_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK10247.1"
                     /translation="MDIQKIPLEKLNPATYNPRKDLKPGDPEYEKLKKSMETFGYVEP
                     IVWNKRTGNIVGGHQRLKILVGQGHDEIDCVVVDLDQTQEKALNVALNKVSGEWDLPK
                     LADLIGELDGELFDISLTGFDAAEIEDLFSKVHDKDVQDDDFDADKALEEIDEPISQI
                     GDVWLLGKHRLVCGDSTEAKTYEVLMAGKKANLCVTDPPYNVNYTGGRENERTIKNDN
                     LDDANFYEFLLAAFKNIYSFLDDGAAAYIFHADTEGLNFRKAFKDVGFHLANVCIWAK
                     QSLVLGRSDYQWQHEPILYGWKPTGRHRWYADRKQTTIWNFDRPTKSELHPTMKPVPL
                     VAYPIQNSSMTNCIVLEPFAGSGSTIIACEQTGRICYAVELDEKYCDVIVKRYVESAG
                     DDGIFLLRDGRKILYQDAI"
BASE COUNT          685 a          712 c          496 g          883 t
ORIGIN      
        1 cggggtgcct ttctctctgg ctggggttcg tttttattca gcggcctttt cccgggattt
       61 ccttctaaaa ccttcagggc tgttggttta ggtttccttc cccgtgtcgc catagccttt
      121 cacctcctat aagaaaaaag agccgatcat tcagctcctt ttgtaaacat tcttaatcct
      181 tcccagctcc ggggtcttgc agataataac aagtcacatt ttcaaatgtc tctgagggta
      241 ttttgctact tcctaatccc cggaaagttt aggttccctc tttttactgc ctcgtgttca
      301 gcatccgccg cctttttata ttctggatcc tccctctctt ttcccttaca atccatacag
      361 atacagtcct tattaaacat agacattatt ctgccattgc ttaaatcttt accacaccta
      421 tcacaatttt tttgtgtaaa aaacttatcc atcttccacc tctcactcta cctccacgta
      481 atccataatt acagccaagg cttcatggta gctgcctgat tgggtgactc tttcagtcat
      541 ctcttgggct tgggccccaa gtccggctct tttcaaggtt cttcgagcaa tacccagcag
      601 attaaaaatg ttgccatcct caccggtaag tttgcatctt ggtttagtca tttaggcttc
      661 ctccccaagc ggatttctga aagcgctatt accggaaaga tttttaagca atacctttct
      721 agcaacttta tactcatcac caatcatccc caagttcaga agccaagtcc taaaagaaaa
      781 tttctcatta tcagtagacc ttacctttgc ggccagattc cgcttacgcc gccgggcatt
      841 aatattggcg agctttaaaa gctgggtggc tgcctgcacc ttctccggat ttttttcata
      901 ggtgtctaac ctaaaaacaa tgatttcatc atcatagtca aagtcaataa aagggcagct
      961 tacccctttc atagcctctt tgaaatgttc tagggtaaac atcctctcct gctgcaaagc
     1021 agcaataatc tcctcactaa ccaggtcgct ttttaaatcc aaagcctttt ttattaaagg
     1081 ttggttgtta tagattaggt gtaaaagatt ccttaagccc ttcccgtcat agccttctaa
     1141 gggaatttgc acattcaaag tttccagtcc catctcctcc ttcgagccac tttccaacaa
     1201 cttttctggc tctactgcct gtccctgcgc atctaaaatg ctgccatgcc ggtcaacggt
     1261 gtagtcacct acttgatagg caaagctggg tgcccctagg taaacagagt ctacttcaag
     1321 gcgttgacta agcaggttta ccagttcttt tcttgacatc gtacatccct cctgtagttt
     1381 ttggtatgta tatacatcac tcaaaaacac aggtaagtca aggattatat ggcatcttga
     1441 taaagtatct tccgtccatc cctaagcagg aaaatcccat catcgccggc agattcaaca
     1501 tatcttttaa cgattacatc acagtatttt tcatccagtt ccactgcgta gcaaatcctg
     1561 cccgtttgct cacaagcaat aatggttgag ccgctaccgg caaaaggctc cagcacaatg
     1621 caattggtca tgctactatt ttgtatggga taagctacca aaggtactgg cttcatagtt
     1681 ggatgcaatt ctgattttgt tggtctatca aagttccaaa tggttgtttg ttttctgtcc
     1741 gcataccagc ggtgtctacc ggtgggcttc cagccataaa ggattggctc atgctgccat
     1801 tgataatcag atctgcctag caccagggat tgtttagccc aaatacagac attggctaaa
     1861 tgaaagccca catctttgaa agcctttcta aaattgagcc cctcggtatc agcatgaaag
     1921 atgtaggctg cagcaccatc atctaaaaaa ctataaatgt ttttaaaggc cgcaagtaga
     1981 aactcataaa agttcgcatc atccaagtta tcgtttttaa ttgttctttc gttttcccta
     2041 ccccctgtat aatttacatt gtagggcggg tcagtaacac atagattggc ctttttccct
     2101 gccattaaaa cttcataggt tttagcctca gtgctgtctc cacatactag tcgatgtttc
     2161 cctaataacc acacgtcccc tatctgggat attggctcat ctatttcttc taaggcttta
     2221 tcggcatcaa aatcatcatc ttgcacatct ttgtcatgga ccttgctaaa taggtcttct
     2281 atctccgcag cgtcaaaacc ggttaaggaa atatcaaaaa gctccccgtc taattcaccg
     2341 attaaatcgg caagtttggg caaatcccac tcaccactaa ctttgtttaa ggcaacgttt
     2401 aaagcctttt cctgggtctg gtctagatcc accacaacac aatcaatctc gtcgtggcct
     2461 tgtcctacca atatttttaa tctctgatgt ccgccgacaa tgttgccggt ccttttattc
     2521 catacgatgg gctccacata gccaaaggtc tccatggatt ttttaagttt ttcgtattcc
     2581 ggatcccctg gctttaaatc cttccgagga ttgtatgtgg ccggatttag tttctctagc
     2641 ggtatttttt gaatatccat gattacatca ccccttttag cgtagatagg gcttttaccc
     2701 ccctccttga atttcgcgaa ttcttgcgtg ttgccccgcc gccgctcctg gggccacagg
     2761 ttgtagggat tgatac
//
LOCUS       DASRNA010000152         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_487926,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000152 DASRNA010000000
VERSION     DASRNA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487926"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VFD08_04170"
     CDS             complement(<1..856)
                     /locus_tag="VFD08_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK10248.1"
                     /translation="MLKEFSMRNYLLLDDLHLEFSSGFSVITGETGSGKSVLLEGLRI
                     LLGQSVTKEDLRNPEEKTYFELIYEDDSEILALSREVFPSGRSISRLNGEIINLQIVR
                     SIFDHKIDFYGQRDDSLLLHRHNQQRLLFTYGKDEIQPILQEIKEYKKEEGLIQNRLS
                     ELRDLSELEKESLKHQLNELEILHIDLEEDTELEENFDEILHGREILEGLQNSKELIE
                     EEVLKGFYRLEDEVFSMKYYSRHQEIHKRLQALRYEIEDLREELADALLSIDVDEAFI
                     NRKEERFSL"
     gene            complement(876..2657)
                     /gene="dxs"
                     /locus_tag="VFD08_04175"
     CDS             complement(876..2657)
                     /gene="dxs"
                     /locus_tag="VFD08_04175"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZK10249.1"
                     /translation="MILSKINSPEELKDLSLLELELLAVQIRNMIISTLEKTGGHLSS
                     NLGTVELTLALHKVFSAPEDRIIWDVGHQTYTHKIITGRRDELKTIRQFQGISGFLKC
                     NESTYDCFGAGHATTSLSAMLGFLIADKAQNKDNYHVAVIGDGALGGGMAFEAMNILG
                     DRGERSMIILNDNEMSIGENVGIISRQLNQIRNNPRYFAVKNKIAPQLNPTMQKGLAS
                     IKKNLRRLVMKSTLFEEMGLRYYGPIDGHNLEELIEAIETLKDYHGPTVLHVLTKKGK
                     GYPFAEENPNLYHGVGPFDSKLAIVEKEKTDFSAVFGQKLLELAKKDEKIFAITAAMV
                     DGTGLRSFQDQMPSRLIDVGIAEESAITMATAMALAGLKPYVAIYSTFLQRGFDQLIH
                     DMALHQAPIVLCIDRSGIVGADGETHQGIYDTSFLSIVPDIHLWAPMDKGELEAMLEE
                     SVSFSTPLAIKYPRGNCPKLKDKTGPIRENQLYSKKSELLVVSYGSMMESLLSLQEEI
                     PFDLYNHRIILPLDIEKLDDLFKNYKRVIVVEENIYTGSLAQKLGALFELEALTLEDG
                     FLAQGSPEQLRKTQGLDKESLRRHILK"
     gene            complement(2657..3505)
                     /locus_tag="VFD08_04180"
     CDS             complement(2657..3505)
                     /locus_tag="VFD08_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZK10250.1"
                     /translation="MAYTKKIEKSLSNLLFRDKNSKLQEAMAYSLLGGGKRVRGQLYL
                     YHLRAFKAPLEEDYSFSAAIEMIHAYSLIHDDLPAMDNDTLRRGKPTNHIAYGEALAI
                     LAGDGLLNLAYEICFDLALKDPDFLLLAQKLARAAGDQGMIYGQMLDLSYQGRTMEEE
                     LIKEMLLNKTGKLIALPIEAAALRAKRGQEEVNRWKKLGMKLGLAFQIKDDLLDVDST
                     AAILGKTPGKDQIDKKNSYVQFHGYDKAQADYKKLQVEILEELALLSSEEKLLGFYHT
                     LLERKY"
BASE COUNT         1016 a          725 c          667 g         1193 t
ORIGIN      
        1 gtaagctaaa tctctcttct tttcgattga taaatgcttc atcaacatca atgcttaaaa
       61 gtgcgtctgc tagttcttca cgaagatctt ctatttcata tcgaagagct tgaaggcgct
      121 tgtggatttc ttgatgacga ctataatatt tcatagaaaa aacttcatct tctagccggt
      181 agaagccctt tagaacttcc tcttcgataa gctctttgga attttgtaag ccctctagta
      241 tttctcgtcc atgcaggatc tcatcaaagt tttcttctag ctctgtgtct tcttccaaat
      301 caatatgaag aatttcaagt tcatttagtt gatgttttag gctttctttt tccagctcac
      361 ttaaatccct tagctcacta aggcgatttt ggatcaagcc ttcttccttt ttatactctt
      421 ttatttcttg aaggatgggc tggatttcgt cttttccata ggtaaaaaga agccgctgtt
      481 gattgtgacg atgaagtagt agtgaatcat ctctttggcc ataaaaatca attttatgat
      541 caaaaataga tctcacaatc tggagattaa taatttcacc atttaaacgc gaaatacttc
      601 taccagaagg gaaaacttct cgactaaggg ctagtatttc gctatcatct tcataaatga
      661 gttcgaaata ggttttttct tctggattac gaaggtcttc ttttgttacg ctttggccaa
      721 gaaggatacg taagccttct aaaagtacag atttaccaga accagtttct ccggtaatta
      781 cactaaatcc cgatgaaaac tccaaatgca aatcatcaag aagaagataa tttcgcatac
      841 tgaattcttt aagcataaaa tgacctttct tggtattatt ttaaaatgtg tcttctaagc
      901 gattctttat ctaggccttg agttttgcgt agttgttcag gactgccttg agcaaggaat
      961 ccatcctcaa gggtaagggc ttctagctca aatagagctc cgagtttttg cgctaaactt
     1021 ccagtataaa tattttcttc tactacaatc actcgcttgt aatttttaaa caagtcatct
     1081 agtttttcta tatccagagg aaggattatc ctatgattat atagatcaaa ggggatttct
     1141 tcttgcagag agagtaaact ttccatcata gaaccatagc tgacaactaa aagctcactc
     1201 tttttgctgt agagttggtt ttctcttata ggaccagtct tatctttaag ctttgggcaa
     1261 tttcctctag gatatttaat ggctaaggga gtggaaaaac ttacactttc ttccaacatg
     1321 gcctcaagct cccccttgtc catgggtgcc cagagatgaa tatcagggac aatacttaaa
     1381 aatgaagtat cataaattcc ttgatgggtt tcaccatcgg ctccgacaat tccacttcga
     1441 tcaatacaaa ggactatggg tgcttgatga agggccatat catgaatgag ctgatcaaag
     1501 cctctttgta gaaatgttga gtagatggct acatagggct tgagtcccgc taaagccata
     1561 gctgtggcca tagttatagc agattcctcg gcaatgccta catcgattag gcgactaggc
     1621 atttgatctt ggaaggacct aagtcctgta ccatcaacca tggccgcagt aatggcaaaa
     1681 attttctcat ctttttttgc taattctaag agtttttgtc caaagacagc actaaagtca
     1741 gttttttctt tttcaacaat agctagcttt gaatcaaaag gccctactcc atggtaaaga
     1801 tttggattct cctcagcaaa gggatagccc ttgccttttt tagtcagcac atgaagcaca
     1861 gtgggcccat gataatcttt gagggtttct atggcttcaa taagctcttc aagattgtgg
     1921 ccgtcaatag gaccataata gcgtaaaccc atttcttcaa aaagggtgga tttcataaca
     1981 aggcgccgta ggtttttctt tatagaagcc aggccttttt gcatggtggg attaagctgg
     2041 ggagcaattt tatttttaac agcaaagtag cgaggattat ttcgtatctg gtttagctgc
     2101 cgagaaataa tccctacatt ttcaccaatg ctcatttcat tatcatttag gataatcatg
     2161 gaccgctcac cacggtctcc taggatattc attgcttcaa atgccatgcc tccacctagg
     2221 gcaccatccc ctataacagc gacatgataa ttgtctttgt tttgtgcctt atcggcaatt
     2281 aaaaacccta acatggcaga aagggatgtg gttgcatgac cagctccaaa acaatcatag
     2341 gtgctttcat tgcattttag aaatccacta ataccctgga attgacgaat agttttaagt
     2401 tcatctcttc taccggtaat tattttgtgg gtataggttt ggtggcctac atcccagata
     2461 atacgatctt ctggggctga aaaaacttta tgaagagcta gggtcaattc aacagttcca
     2521 agattggagg aaagatgtcc accggttttt tccagcgttg aaataatcat atttctaatt
     2581 tgaacagcta gtagctcaag ctccaaaaga gaaagatctt taagttcttc aggggagtta
     2641 attttcgaaa gtatcattag tatttccttt ccaaaagagt atgataaaat ccaagaagtt
     2701 tttcctctga actaagcagt gccagctctt ctaaaatttc tacttggagt tttttataat
     2761 ctgcttgggc tttatcataa ccgtggaact ggacatagga gttcttttta tctatttgat
     2821 ccttaccagg agttttaccc aaaattgctg ccgtggaatc gacatcaagc aaatcgtctt
     2881 ttatttgaaa agcaaggcct aacttcattc ctagtttttt ccaacgattt acttcttctt
     2941 ggcctctttt agcccgaaga gctgctgctt caataggaag ggctattagt tttcctgttt
     3001 tattcagtag catttctttt ataagttctt cttccatggt gcgaccttgg taactaagat
     3061 caagcatctg accgtagatc ataccctggt ctcctgcagc cctagcaagc ttttgagcta
     3121 gtagtaaaaa atcaggatcc ttcagggcca aatcaaaaca tatctcatag gcaagattaa
     3181 ggagcccatc tcccgctaag atagccaggg cttcaccgta tgcaatatga tttgttggtt
     3241 tacctcgacg tagagtatca ttatccatag ccggtagatc atcatgaatc agagaataag
     3301 cgtgtatcat ctcaatggcg gcactaaagc tataatcttc ttctaaggga gccttaaatg
     3361 cccttagatg ataaaggtat agttgtcctc gaacacgttt tcctccacct aaaagagagt
     3421 aggccatggc ttcttgtagt ttgctatttt tatctctaaa gagaagattt gataggctct
     3481 tttcaatttt cttggtgtag gccattaaac tcttcctcaa tttttttata atatttttgc
     3541 aattctttgt aaatttctac ggcttcttta tataaatcaa gagctttatc taaggccata
     3601 g
//
LOCUS       DASRNA010000153         6400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_642028,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000153 DASRNA010000000
VERSION     DASRNA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642028"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..508)
                     /locus_tag="VFD08_04185"
     CDS             complement(134..508)
                     /locus_tag="VFD08_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZK10251.1"
                     /translation="MKILISNSSSIPIYEQIKDSIISQILSDDLKEGEKLTSIRSLAK
                     DIRISIMTVKRAYDELEKEGYLVTHQGKGSYISLKSSELAKEMKQKEIEDYIFKIVDI
                     SKRHEIDKEEILEIFDLAYRGE"
     gene            complement(963..2075)
                     /locus_tag="VFD08_04190"
     CDS             complement(963..2075)
                     /locus_tag="VFD08_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HZK10252.1"
                     /translation="MQIALTKKLADVMAVKSLAPLDEENPLFSWTANWTKVWDNRRAD
                     DTLVLVNNATRFAVGIYEVKRKDLKKNPKITAQMMRDAISNTLLATNYNPELVEEYMR
                     LAGEVEFVRNQNRQTTSWIVKAGLMCVNYIAHEYNGIEKMYSDTIGVRVNRLFVNYSG
                     KNKDLFYPCEVMAKALSELSEKPIYKYRAFELLVSLDLEVYKAVRRIIVPANLEFIKL
                     HKILQSIYNWKDYHLYDFVIMDDKDPEAATLIVPYEEDLEYNEDAVLMKGLVLSDFLP
                     ESKRILYTYDMGDCWSHKIQLVRVLEDYDKESPYLLEARGQSPPEDVGGLGGFLEFRK
                     IMMDPNHPQYEETLEWVGYWSWDLPSWQSYPGFIDV"
     gene            complement(2353..3222)
                     /locus_tag="VFD08_04195"
     CDS             complement(2353..3222)
                     /locus_tag="VFD08_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK10253.1"
                     /translation="MGKKSYLEVHMAVFLFGMTGLFAKFIELNPIIIALGRVFVAAIF
                     IFFWLIFSKESLKLNHFSDYKKVTAMGVLLAIHWTTFFAAIQLSNVAVGILTFSTFPV
                     FVSLFRPFINKEQISKKEVIFGFITIVGILFIVPLKDVFSDTMVGAMIGVLSGAVYAI
                     FTIFNEKLVKIYSGKKVAFYEQVAATIFLLPSLFIIKPLMTSKDILLIILLGTLFTGV
                     AHTMFINGLKEVSAYMASIITMLEPLYSIILAYFLLGEKLHMNTAIGGLIILSTVVFI
                     SLDNVKEKKVKRS"
     gene            complement(3489..4037)
                     /locus_tag="VFD08_04200"
     CDS             complement(3489..4037)
                     /locus_tag="VFD08_04200"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZK10254.1"
                     /translation="MYTLKEIESYAHKCRRCPLHKTRTKLVFGAGNPKADILFVGEAP
                     GFYEDKSGQVFVGKSGQLLDKMLASIDLKREDIYIANIIKCRPPDNRSPTEEESTSCI
                     EFLRWQVKIIQPKIIVCLGAVAAKNIISPDFRVSRQRGVWISRGSYSIIASYHPSALL
                     RDESKKTEAWEDLKKIRQKLDE"
     gene            complement(4157..4432)
                     /locus_tag="VFD08_04205"
     CDS             complement(4157..4432)
                     /locus_tag="VFD08_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HZK10255.1"
                     /translation="MSSLLGYYGVAINTLALTITVYDKWASRHLPTKRTKEATLLLIS
                     TLGGSPGMFLTMILIGHKIRHAKFMLGLPILFLIQFFLLKNLGLIVI"
     gene            complement(4585..5397)
                     /locus_tag="VFD08_04210"
     CDS             complement(4585..5397)
                     /locus_tag="VFD08_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10256.1"
                     /translation="MKKNLFVIVLALLMAVALMACSAEEAVETAQEPKEESAQVEEVV
                     EEAEEEIEEAEEDKEEALEDSTEGADLLSQLSGQMPKSLKMKMTTESFGMVTSSISYY
                     EGDNTRTEIMIEGLGTSVIIYNADEEIIYSYVEGEELATRIMGADIDSAEEIGLTVDI
                     TSKYTELTEEIPDNMIARKEELDGEEVVYIEVTEVDDEMGEMLIKMWYSTKYNTPLKY
                     EVLVDGQSFMLLTVTELETDMDIDSSIFMPPMGMEFIDVDLDSMLEMTELGD"
     gene            complement(5721..6107)
                     /locus_tag="VFD08_04215"
     CDS             complement(5721..6107)
                     /locus_tag="VFD08_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZK10257.1"
                     /translation="MKKTTIVFLLVLLLLLVGCSRSSIEEESGYKKISAEEAKEMIDT
                     EDLVILDVRTKEEYDQAHIEGAILIPDYQLKEQAESKLPNKEEKILLYCRSGNRSESA
                     AKILIEMGYSKVYDFGGIIDWPYELK"
     gene            complement(6143..>6400)
                     /locus_tag="VFD08_04220"
     CDS             complement(6143..>6400)
                     /locus_tag="VFD08_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZK10258.1"
                     /translation="VYEGMIVGLNSRNEDMIVNPNRAKKMTNVRASGTDDAIKLTPHR
                     VFTLEGALEFINDDELVEITPLNIRIRKKILKELDRKRSGR"
BASE COUNT         1865 a         1366 c          973 g         2196 t
ORIGIN      
        1 gttttacccg atccattttc cccaataaag cctgttatga ttccttcttt tatctctaaa
       61 tccatcttct ttatttcaaa gccactttta tattgttttg aaaggtttct tatctcaatg
      121 gcatttaaca tttctattca cctctgtaag caagatcaaa tatctctagg atctcttcct
      181 tatctatttc atgtcttttt gatatatcaa caatcttgaa gatatagtct tcaatttctt
      241 tttgtttcat ttcttttgcc agttcactgc tttttaagga gatatagctt cccttgcctt
      301 gatgagttac aagatagcct tctttttcta attcatcata ggctcttttt accgtcataa
      361 tacttatgcg tatatccttg gctaaagatc ttattgatgt tagtttttct ccttctttta
      421 agtcgtcact taaaatttga gaaattatgc tgtccttaat ttgctcataa attggaatac
      481 tactggaatt actaattagt attttcactg cttcacctca ctgttatatg taatatataa
      541 cagtgagtaa cagttgtcaa gaagaaaata atacgtctaa agtccttttt tatcctcttt
      601 attctaataa aaaatatagg ctcccagcct atattaaagg tctatcctaa tattttttaa
      661 cccataaaaa accaaagtag ctccttagca agcaaggctc tttggcccct ttttttctcc
      721 tataaagaca agacccctat tatagatcca accatagtat tgctaaacat atccctcagc
      781 ggaataataa acaagatacc aactatgcta ataaaaaaaa ggtttttagc tccatattag
      841 aagcttctaa aaactcccct aactaggaag cctaggggag gataaaagct tgaaccggat
      901 gaaaatttta cctttttcct atagactgta tgctcattag actcctagct acctgtactg
      961 ctttatacat caataaatcc aggataactt tgccaggaag gaagatccca agaccaatag
     1021 cccacccatt ccagcgtttc ttcatactgg ggatggttag gatccatcat gattttacga
     1081 aattctaaaa agcccccaag gccccctaca tcctctggtg gggactggcc ccttgcttct
     1141 agcaagtagg gtgattcctt gtcatagtcc tctaggactc ttaccagttg aatcttgtgt
     1201 gaccaacagt cccccatgtc ataggtataa aggatacgct tactttcagg taaaaaatcc
     1261 gataagacta gacccttcat taaaactgca tcttcgttat attctaaatc ttcctcataa
     1321 ggaacaatta aggtagcggc ctcaggatct ttatcgtcca taataacaaa gtcatatagg
     1381 tgataatcct tccagttata gatcgattgc aatattttat gcagttttat aaattctaaa
     1441 ttagcgggta caataattct acgtactgcc ttatagactt ctaaatctag gcttacaagt
     1501 aattcaaagg cgcggtattt ataaattggt ttttcgctta gctcagacag ggccttggcc
     1561 attacctcgc agggatagaa tagatctttg tttttacccg aataattaac aaacagccgg
     1621 tttacacgaa ctccaatggt atcactatac attttttcaa tgccattata ttcatgtgca
     1681 atataattta cacacatcag ccccgctttt actatccagg atgttgtttg cctattttga
     1741 tttcgtacaa attctacctc acctgctagg cgcatatatt cctctaccag ttcgggatta
     1801 taattcgtag caagtagtgt gttagaaata gcgtctctca tcatttgtgc tgtaatttta
     1861 gggttctttt ttaaatcctt acgcttgact tcataaatcc caacggcaaa acgagtggca
     1921 ttgttcacca gtaccagggt atcatctgct ctacgattat cccatacctt agtccagttt
     1981 gccgtccagg aaaaaagcgg gttttcttcg tcaagtgggg ctaaagactt tactgccatg
     2041 acatcggcca gcttttttgt cagtgcaatt tgcatttttc ttcctttccc tctatcactt
     2101 gatttggagt aatcagctct tacttctatt agcaaagata gtctaaaaaa ctttccttct
     2161 tctatccata gtctactata agtctattta aaataaaaga catatcctga gtttaggagt
     2221 taaaagtaaa taaaacagcc ctaacccttg ctagaacagg cttttagctg ttttttcctt
     2281 gtggactata tctatttgct tcttggtttt tatatcactt tgagaaggct cactagtctc
     2341 tcttacaagg ctctaggacc tttttacttt cttttctttt acattatcta gggatataaa
     2401 aacaacagtt gaaagaatga ttaggccacc aatggcggta ttcatatgta atttctcacc
     2461 aagtagaaaa taagcaagta ttattgagta caagggttct agcatggtga taatgctagc
     2521 catataggca gaaacttctt ttaacccgtt aataaacata gtatgggcca ccccagtaaa
     2581 taaggtcccc aacaaaataa tcagtagaat atccttactt gtcataaggg gttttatgat
     2641 aaacagactt ggaagtaaaa atatggttgc cgcaacttgt tcataaaagg ccactttttt
     2701 tccactgtaa atctttacca acttttcatt gaagatggta aaaatagcat aaaccgctcc
     2761 ggataagacc cctatcatag ctcctaccat agtatcgcta aagacatcct ttagtggaac
     2821 aataaacaaa ataccaacta tggtaataaa gccaaagatg acctcttttt ttgatatctg
     2881 ctccttattg ataaagggcc taaataagct aacaaataca ggaaaggtag aaaaggtcaa
     2941 aatccccact gctacattgg ataattgaat ggcagcaaaa aaagttgtcc aatgaatagc
     3001 caaaagaact cccatagcag taaccttttt ataatcagaa aagtgattca atttaaggga
     3061 ttccttacta aagattagcc aaaaaaatat aaatatagct gccacaaaaa cccgccccag
     3121 ggcaatgata attgggttta attctataaa tttagcaaaa agtcccgtca ttccaaaaag
     3181 aaagacagcc atgtgaactt ccaaataact tttttttccc atacaaatcc tctttcatca
     3241 aggcccaaga aaaaccaccc gtcctatcca ataggttttt gattttctag ggccctcccc
     3301 tcgaagttct ggggctccat agcctatatc atatcctatt atagtagctt attctatcta
     3361 aacttctagc tagtaaaaag cctagaatca tataggccct atcctagtct ttctaaatta
     3421 ataaaaacta ggacaagaaa agcaatccca gttttttaag ccatataatt tatactgtcg
     3481 atctttactt attcatctag cttttgacgt atttttttca aatcttccca ggcctctgtt
     3541 tttttactct catcgcgcaa gagggccgat gggtgatagc tagcaataat agagtagctt
     3601 cctctactaa tccaaacccc tctttgccga gatactctaa aatcagggct tataatgttt
     3661 ttagctgcca ccgctcctag acaaacgata atttttggtt gaataatctt aacctgccag
     3721 cgtaaaaact ctatacagct agtagactct tcttctgtgg ggcttctatt atctggtggc
     3781 ctacatttga taatatttgc aatgtaaatg tcttctcttt ttagatctat ggaggctagc
     3841 attttgtcaa gaagctgccc tgattttccc acaaagacct ggcctgattt atcctcgtaa
     3901 aaaccaggag cctccccaac aaaaagaata tcggccttag gatttcctgc tccaaaaacc
     3961 agcttggtcc ttgttttatg taaggggcag cgcctgcatt tatgggcata ggactctatt
     4021 tcttttaatg tataaattgc caccaactcc cttcggtctt taactttata agctcttttg
     4081 tctttattgt agcataataa aaagctccca cccaagtttc ctttgataag agcttttcat
     4141 taatttaagg taaagattaa atgactataa ggccaaggtt ttttaagaga aaaaactgta
     4201 taagaaaaag gatgggaagg ccaagcataa attttgcatg ccttattttg tgcccaataa
     4261 gtatcatggt taaaaacatc cctggtgaac ctcccaaggt agaaataagc aaaagagtcg
     4321 cttcttttgt tctttttgtc ggtaaatgcc tgctagccca cttatcataa acagtaatcg
     4381 taagggctaa ggtatttata gctaccccgt aatagcctag taaagagctc atagcttttc
     4441 ctttctatat ctcctctctc ccttcttcta agtgcaataa aaaaaaggag gttaagtaaa
     4501 aatacgataa aagccctaaa actgccataa acagcactag ggcttttatc atatctatta
     4561 tacaaggatt cactggacct gggattaatc tcccagctct gtcatctcca gcatagaatc
     4621 aagatctaca tcaataaact ccatacccat aggaggcata aagatactac tatctatgtc
     4681 catatctgtt tcaagctcag taactgttag taacataaag ctttgtccat ccacaagaac
     4741 ttcatatttt agcggagtat tgtatttggt tgaataccac atcttaataa gcatttcacc
     4801 catttcatcg tcaacttcag tgacttcaat atagactact tcttctccgt ctaattcttc
     4861 ctttcttgct atcatattat ctggaatttc ttcggttaac tctgtgtatt tactagttat
     4921 atctactgtt aaaccgattt cttctgctga gtcaatatcg gctcccataa ttctagtagc
     4981 cagctcttct ccttctacat aactgtagat tatttcttca tctgcattat atataataac
     5041 gctggtacct aaaccctcta tcattatttc cgttcttgta ttatcgcctt cataataaga
     5101 gatggaactg gtaaccatgc caaaactttc cgtggtcatt ttcattttta gagacttggg
     5161 catttgacca gaaagctgac ttaacaagtc agctccttct gtagagtctt ccagagcttc
     5221 ttctttatct tcttcggctt cttctatttc ttcttcggcc tcttcgacaa cttcctctac
     5281 ttgggcactt tcttcttttg gctcttgagc agtttctacc gcttcttctg ctgaacaagc
     5341 cattagagcc actgccatta gtagggctaa aacaattaca aacaaattct ttttcattgg
     5401 ggaatctcct ttacttcatt ctgaatcttt ctactatcaa gtattcattg acgtaaatca
     5461 ggatttagta tcccaataaa aattgttata actctcatca caatacaatc cgaattcctt
     5521 ggcccttgtc tttcgctttt acttgctctt gccttattca ttgataattt catcaagaat
     5581 ttaggtgttt agtctactaa taaaaaaacc cttttttagc aaagaccctg ctgaaatcac
     5641 ttatcttttt atggtgatta ttgtataccc caaagtttta tattttctca tctttttttg
     5701 cttcttctat ctaggttaat tcactttaac tcatagggcc agtctattat gcccccaaaa
     5761 tcatagacct tggagtaacc catttcaatt agtattttgg cggcagattc gcttcgattt
     5821 ccgcttctac aatacaaaag gattttttct tctttatttg gcaatttgct ttctgcctgc
     5881 tctttaagtt gataatctgg aataaggatg gctccttcaa tatgggcctg gtcatattcc
     5941 tcttttgttc taacatctaa aattacaaga tcctctgtgt caatcatttc ttttgcttct
     6001 tctgcagaaa ttttcttgta cccagattct tcttctatgc tacttctact acatccaact
     6061 agtagaagca gtaatacaag cagaaagaca atggtggttt ttttcattat agtctacctt
     6121 ttatttctat ggggttagtt ttttatcttc ctgagcgctt tcgatcaagt tctttcaata
     6181 tttttttgcg aattctgata tttaaaggtg taatttctac cagttcatca tcattaataa
     6241 actctagggc accttctagg gtaaagactc ggtggggagt tagtttaatg gcgtcatccg
     6301 ttccagatgc acgcacattg gtcattttct ttgctctatt ggggtttaca atcatatctt
     6361 catttcggct atttaggcca acaatcattc cctcatatac
//
LOCUS       DASRNA010000154         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_48014,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000154 DASRNA010000000
VERSION     DASRNA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48014"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..148)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(208..873)
                     /locus_tag="VFD08_04225"
     CDS             complement(208..873)
                     /locus_tag="VFD08_04225"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004814360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HZK10259.1"
                     /translation="MGYFSNVSALKKDILQETIATLYMTSFSALIAGFIGLILGIVLV
                     VTQRQALLENRLLYTVTDKLINLLRAIPFIILMALIAPVTRWIVGTRIGNKAAIVPLV
                     FSAAPFFAKQVEQALSSLDYGLIEAAISMGDGPMDIIFSVYLKEGFNNLVRASAITLI
                     SLLGLTTMAGAIGAGGIGKLAIAVGYNTYKDDIVLVSVVIILILVYSIQGLANLLIKK
                     NTH"
     gene            complement(857..1888)
                     /locus_tag="VFD08_04230"
     CDS             complement(857..1888)
                     /locus_tag="VFD08_04230"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015777238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HZK10260.1"
                     /translation="MVELKDITVAFSDTIACQNVSLHIQKKDIFGIVGFSGAGKSTLV
                     RCINLLQRPDYGEVRISGQDIMKLNEKELRKKRRKISMIFQHFNLFESRSILENVIYP
                     IRKDKISKKEKHKKALELLKLVGIKDKKDSYPSELSGGEKQRCAIARALCSNPDILLC
                     DEATSALDPTSTQKILTLLRELNEKLDLTIVIITHEMEVVKEICNKCAVMDKGEIVEQ
                     ASTIDLFSNPQQDITKEFVKKANRFWENLERLQAKNLAIDKKEILAELKYFGESTTEP
                     FIIELYKKWEIETNILFGDIDFISGVPVGNLIVTFSGKTQNLDKVLESLEDKSITINV
                     LGGIDGILF"
     gene            complement(1926..>2699)
                     /locus_tag="VFD08_04235"
     CDS             complement(1926..>2699)
                     /locus_tag="VFD08_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK10261.1"
                     /translation="PEASETPGAYEKPEVIRIGVVGENNEDWLDVINRLEEEEGLKVE
                     LITFTDYNQPNEALANGDLDLNAFQHKKFLNTYVEESGNELVGIADTIRAPLGIYSNK
                     IASLDELKKGDSIAIANDASNGSRALLVLRNAGLLEIDGKKAGDIVVPSDITKNELEI
                     EIIQLDAAQTSRSLDDLTAAFVNADLAISAGISPIEDAIYTEELGPDTDIYVNIIAAR
                     AKDKDNPYYKKIVDYYQSDQTAEILLETFKGAKVPAWKE"
BASE COUNT          753 a          568 c          463 g          915 t
ORIGIN      
        1 atgatcactt tactcgctga ccttcccatc aaatagacag tggttttcag ctttcatcac
       61 atttacagta gcgggggctg tggaagaatt gcacttcctt cccttttaat ataaaagaat
      121 tttaataacc tataaacaca atatgtagtt gttattaatc ctaccatata ctctatcttg
      181 tttcaagccc ttttggccta agggttctta gtgggtattc ttttttatta agagattggc
      241 aaggccttga attgagtaga ctaaaataag aataattact acgctcacca aaacaatatc
      301 atctttatag gtgttgtagc ccacggctat ggccagcttt cctattcctc cagcccctat
      361 agctcctgcc atggttgtga gtccaagaag gctaatcagg gtaatagctg atgccctcac
      421 caagttatta aaaccttcct ttaaataaac agaaaaaata atatccattg gaccatcacc
      481 catagaaata gcagcctcta tgagcccata atccaaacta gatagggctt gctctacctg
      541 ttttgcaaaa aatggagctg ctgaaaatac aaggggcaca atggctgcct tgttgcctat
      601 cctggttcct acaatccagc gggtaacagg ggcaatcaga gccattagta taataaaagg
      661 aatggctcta agtaggttga ttagtttatc cgtaaccgta taaagcaatc tattttcaag
      721 cagggcctgt ctttgggtga caaccagaac aatccctaag ataaggccga taaagccagc
      781 aatcaaagcc gaaaaggatg tcatatagag agtggctatc gtttcttgta aaatgtcttt
      841 ttttaaggca gaaacattag aaaagtatcc catctattcc tcctaacaca ttaattgtaa
      901 tacttttatc ctctaggctc tctagcacct tgtctaggtt ttgggttttg ccactaaagg
      961 ttacaatcaa gtttcctaca ggaactcctg atataaaatc gatgtcccca aaaagtatat
     1021 ttgtttcaat ctcccatttt ttgtagagtt ctataataaa aggttctgtt gtgctttcac
     1081 caaagtattt taattcagct aaaatttctt ttttatcgat agctagattc ttagcctgca
     1141 atcgttccag gttttcccaa aaacggtttg cttttttaac aaattctttt gtaatgtctt
     1201 gctgcggatt ggaaaaaaga tcgatggtgg aagcttgctc tactatctct cctttatcca
     1261 tcacagcaca tttattgcaa atttccttta ccacttccat ctcatgggtg ataataacaa
     1321 tggtcaaatc taacttctca tttagttctc ttaaaagggt taatattttt tgggttgagg
     1381 tgggatctaa agcacttgtt gcttcatcac ataagagaat atctggattg gagcagaggg
     1441 ctcttgcaat ggcacacctt tgtttttctc ccccggaaag ctccgaggga tagctgtctt
     1501 tcttgtcttt tatcccaaca agtttcaaaa gctcaagggc ctttttatgc ttttcttttt
     1561 ttgatatttt atcttttctt atggggtata taacgttttc cagaatgctt ctggattcaa
     1621 ataaattaaa gtgctggaat atcatggaga tttttcttct ttttttacgc agttcttttt
     1681 catttaactt cataatatct tggccagaaa tcctaacctc cccataatct ggcctctgca
     1741 gaagattaat acagcgaacc aaggtagatt ttccggctcc ggaaaatccc acaataccaa
     1801 aaatatcttt cttctgaata tgcaaggaaa cattttggca agcaattgta tcagaaaatg
     1861 caaccgtgat atcttttaat tcaaccattt ttcacccttt ctagcagtct agaagttcta
     1921 gttttctact ctttccaggc tggtactttt gcccctttaa aagtctcaag tagaatttct
     1981 gctgtctgat cactttgata atagtccact atttttttat aatagggatt gtctttgtct
     2041 tttgctctag cggcaattat atttacatag atatctgtat caggtccaag ctcttctgtg
     2101 taaatggcat cctcaatggg agatatacct gcactaatgg ccaggtctgc attaacaaag
     2161 gcggctgtta gatcatctag gcttctgctt gtttgagctg catccaactg gataatctct
     2221 atttctagtt cgttttttgt aatatctgag ggcactacta tatcccctgc ttttttgcca
     2281 tcaatttcaa gtaaaccagc atttcttaat actaaaagtg cacgggaccc attggaagca
     2341 tcattggcaa tagcaattga atcccctttt tttagttcat ctagtgaagc gattttgttg
     2401 gaataaatcc ccaggggagc acgaattgta tcggcaattc ccacaagctc attgccactt
     2461 tcttctacat aagtatttag gaatttcttg tgttgaaagg catttaagtc caaatcccca
     2521 ttggcaagag cttcatttgg ttgattgtaa tcggtaaatg taatcagttc taccttaagg
     2581 ccttcctctt cttccaaacg atttataaca tccaaccaat cttcattgtt ttcacccact
     2641 acaccaatcc ttattacttc aggcttctca taggcccctg gtgtctctga agcttcagg
//
LOCUS       DASRNA010000155         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_570062,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000155 DASRNA010000000
VERSION     DASRNA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570062"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFD08_04240"
     CDS             complement(<1..264)
                     /locus_tag="VFD08_04240"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZK10262.1"
                     /translation="MTLKIVIALGGNALGHTPKEQQQLVKQSVKPIVDFIQAGHDVIV
                     AHGNGPQVGMINLAFEEARKINKHIPELGFAECSAMSQGYIGYH"
     gene            complement(281..889)
                     /gene="udk"
                     /locus_tag="VFD08_04245"
     CDS             complement(281..889)
                     /gene="udk"
                     /locus_tag="VFD08_04245"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HZK10263.1"
                     /translation="MLKLLGIAGGTASGKTTIVRELMETLGDKGQLIGLDAYYLPFYE
                     MDYEQRTRQNYDHPFSIDMDRLYGDLLELKQGRGVDIPVYDYVNYTRSDRTHHIYAHG
                     VLIVEGLFALYDPRIRKLFDLSVYVQADADERLIRRIGRDMKSRGRSLESILQQYQVT
                     VKPMHEAFVAPTKRYADIILPGGAQNRRGVELLKSHLRAMIL"
     gene            complement(890..2983)
                     /locus_tag="VFD08_04250"
     CDS             complement(890..2983)
                     /locus_tag="VFD08_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HZK10264.1"
                     /translation="MQILKRLAQELDLELIQVEKTVELMDQGNTLPFIARYRKEVTGN
                     LEDVQLRELEEKLTFYRNLEERKATVIRLLKEQELLTDKLEKSIEKSSSLQQIEDIYR
                     PYRPKKRTRATVAIEKGIEPLADFLLAGQGSQEELLAFAQEFISQEKEVASAQEALVY
                     AQDIIADRLSDDLKARGIVRRESFLSGKFLSQALEDASAVYEIYWDFSEPLRTMKPHR
                     TLAIFRGEKEGSLRIGFDFPDEEIIEKLKYLFRGKSHPFEIYIDEALEDGYKRLLRPS
                     IETETRNALKEIADRESIDVFKKNLRPYLMQAPLPGQVVMGIDPGFRTGCKFAVVGPM
                     GELLDYGTIYPTKPREDIEGSKKVFSLMVEKYDVSLFAIGNGTGSRETEQVVASFIEE
                     EEKTLYYCIVNESGASIYSASALAKEEFPDLDVSIRGAVSIARRVQDPLAELVKIEPK
                     HIGIGQYQHDVNQKLLDESLEKVVEDCVNAVGVNLNQASFRLLSYVAGISNNLAKNIV
                     KYKEEQGGFKSRKELMEVRGLGPKTFEQCAGFLRVPESAEPLDHTAVHPESYSVAKPL
                     LGRQIDTRNLAALAEELEVGLPTLKDIIEELKRPGRDPREEMPKPILRSDVLSLNDLK
                     EGMVLNGTVRNVVDFGAFVDIGVKEDGLVHISKISDKFVKHPSDVLEVADIVKVKIIG
                     IDHKRNKISLSMKDI"
BASE COUNT          816 a          715 c          552 g          988 t
ORIGIN      
        1 atggtatcca atatatccct gactcatagc gctacattca gcaaagccta gctcaggaat
       61 atgtttgttt atctttctag cctcttcaaa ggcaaggttt atcatgccaa cctgagggcc
      121 attgccgtga gcaacaatca catcatgccc cgcttggata aaatccacta tgggctttac
      181 actttgtttt accagctgct gctgctcttt tggagtatgg cctaaggcat ttcctcccag
      241 tgctataaca attttcaaag tcaacctctt tctggaaatt ttaaagtatc atggcccgaa
      301 gatggctctt taaaagttcc actcctcgtc ggttttgtgc cccacctggt aaaataatat
      361 cagcatagcg cttggtgggt gcaacaaagg cctcatgcat tggcttaacg gttacctggt
      421 attgctgcaa gatagactcc aagcttcttc ctctactttt catatcccta ccaattcttc
      481 ttattagtct ttcatcggcg tcggcttgga catagacaga aagatcaaag agctttctaa
      541 ttctagggtc ataaagtgca aatagtccct ctacaataag aactccatga gcatagatat
      601 gatgggttcg atcgcttctt gtatagttca cataatcata aacgggaata tctactcccc
      661 ttccctgctt gagttcaaga agatcgccgt ataagcggtc catatcaatg gaaaagggat
      721 ggtcatagtt ttgtcttgtt ctctgctcgt agtccatctc gtaaaaggga agatagtagg
      781 catcaagtcc aattagctgg cccttatctc ccaatgtctc cataagctct cgaacgatgg
      841 ttgttttacc tgaagctgta cctccagcta tccccaaaag cttaagcatt tatatatcct
      901 tcatagaaag tgaaatctta ttgcgtttat gatcaattcc aatgattttt accttaacaa
      961 tatccgctac ctcaagcaca tcactggggt gtttaacaaa tttatctgat atctttgaaa
     1021 tatggaccag tccgtcttct ttgactccaa tatctacaaa agcaccaaaa tccaccacat
     1081 tgcgtactgt tccattcaga accataccct ccttaagatc attaaggctg agcacatcac
     1141 ttcttaaaat gggttttggc atctcttctc tggggtctct tcctggtctt tttagttctt
     1201 ctataatatc ttttaaggtt ggaagtccca cctctagttc ctctgccaaa gcggctaaat
     1261 tcctagtatc tatttggcgt cctagaagtg gtttagcaac agagtaactc tcgggatgga
     1321 cggctgtatg atcaagaggt tctgcgcttt ctggcacccg taaaaaacca gcgcactgct
     1381 caaaggtttt tggtcctagg ccccttacct ccataagttc ttttctgctt ttaaaaccac
     1441 cttgttcttc tttatattta acaatatttt tggcaagatt gttgctgata cctgcaacat
     1501 agctaaggag tctaaaggag gcctgattaa gatttactcc tacagcattc acacaatctt
     1561 ccactacctt ctctaggctc tcatctaaga gtttttgatt tacatcgtgc tgatactgac
     1621 caatccctat atgcttgggc tcaattttta ccagctctgc caaaggatct tgtacgcgac
     1681 gagcaatact aacagcgcct cttatactaa catcaagatc aggaaactct tccttagcaa
     1741 gggcagaggc tgaataaatt gaggctccag attcatttac aatacaatag taaagggtct
     1801 tttcttcttc ctcgataaag gaagccacta cctgttcagt ttctcttgag cctgttccat
     1861 tacctatggc aaaaagactg acatcatatt tttctaccat gagagaaaac acttttttac
     1921 tgccctcaat atcttctctt ggttttgttg gataaatagt cccataatca agaagttccc
     1981 ccatggggcc cactacagca aatttacatc cagttcgaaa tccagggtcg atacccataa
     2041 ccacctggcc aggaagtggg gcttgcataa gatagggtcg taggtttttt ttgaaaacat
     2101 ctatggactc cctatcagca atttctttta aagcgtttct tgtttctgtt tctatactgg
     2161 gtcgaagaag ccttttatat ccatcctcta gggcctcatc tatatagatt tcaaagggat
     2221 ggctttttcc tcggaacaag tatttgagtt tttctataat ctcttcgtcg ggaaaatcaa
     2281 agccaatgcg caaagaacct tccttttctc ctctaaaaat agccaaggtt cggtggggtt
     2341 tcatggtccg tagaggttca gaaaaatccc agtatatctc ataaacagca cttgcatctt
     2401 ctagggcttg actaagaaac tttccgctta aaaaagattc tcttctaacg attccccggg
     2461 ccttaagatc atcacttagt ctatctgcta ttatatcttg ggcataaact agggcttctt
     2521 gggcactggc tacttctttt tcttggctaa taaattcttg agcaaaggct aaaagttctt
     2581 cctgcgaccc ttggcctgct aaaagaaaat ctgccagagg ctcgattcct ttttctatcg
     2641 caactgtggc cctggttctt tttttaggtc ggtagggccg gtaaatatct tctatttgtt
     2701 gcaagctaga agatttttct atggactttt ccagcttatc cgtcaaaagc tcctgctctt
     2761 ttaaaagtcg gataactgta gccttgcgct cctctaagtt tctgtagaaa gtcaacttct
     2821 cttccagctc acgaagctga acatcttcca gatttcctgt tacttcctta cgataacgtg
     2881 caataaaagg aagtgtattc ccctggtcca taagttctac cgttttttcg acttgaataa
     2941 gttctaagtc tagttcctgc gcaagtctct ttaaaatctg cattctaacc tctccttttt
     3001 ttatattggg ggtataaaaa tagtagcaat ataagcccca ggaaaaacaa agtaattgga
     3061 tacattatgt a
//
LOCUS       DASRNA010000156        13139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_279165,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000156 DASRNA010000000
VERSION     DASRNA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13139
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279165"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VFD08_04255"
     CDS             complement(<1..1189)
                     /locus_tag="VFD08_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HZK10265.1"
                     /translation="MQKNMFFWILILTVLVFVFWSSLQPTETAVELNFTDVIMKIETN
                     QVESMSVKDNVVGGFLKDGTEFAVFVPGMFAEYYGTEVLDAARNQDIPIETAAPDVEP
                     FWVSILPTLLIMGIMLFAWFMLMNSSQGGANKAMSFGKNRAKLHKQDTGRRITFSDVA
                     GLHEEKEEMMEIVDFLKSPDRYRKLGARIPKGVLMSGLPGTGKTYLSRAIAGEANVPF
                     YSISGSDFVEMFVGVGASRVRDLFNTARKDTPCIVFIDEIDAVGRHRGAGLGGGNDER
                     EQTLNQLLVEMDGFGDQEGIIIIAATNRPDILDPALLRPGRFDRQVYVGVPDVKEREA
                     VLKVHSKDKPLNEEVDFGVIAKRTPGFTPADLENMMNEAALITARKKQPEITMEILEE
                     ATTK"
     gene            complement(1244..2491)
                     /gene="tilS"
                     /locus_tag="VFD08_04260"
     CDS             complement(1244..2491)
                     /gene="tilS"
                     /locus_tag="VFD08_04260"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZK10266.1"
                     /translation="MKLIPQLKGKILLAFSGGPDSVVLLDLFLKEGCEVELFHLDHNL
                     RENSKDDARFCANLAKEKKLLLHSYSLDVEEYCREKGLGIEEGARNLRYDFLRKIKKE
                     RNLDYIATAHHLDDQMETFFMNLFRGSGTAGLGGIHPVKGDLYRPLLSFRKEEIMEYA
                     KKNKLAYVIDQTNLEPIYSRNRLRLDLIPKIEEEYAPNLSKRLLTSMEILREENAFIN
                     ELLDEKIDWDKELYGVEEIRQLHPVLKKAFIRRKFSLNYAQTQEAMDILEERSRGEKH
                     FSEGLLRREQDFFYIGPYIKKPSVSHKLLLGTNELEGYTFEIRSAQKPLFTENTHSIP
                     EELLEEPLVLRHRRPGDMFQPSGLQGKKKLKDYFIDEKIPRSQRDKYWLLASAETVFW
                     IIGLRKAQISTTAQNYLQIFIEK"
     gene            complement(2488..3318)
                     /locus_tag="VFD08_04265"
     CDS             complement(2488..3318)
                     /locus_tag="VFD08_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10267.1"
                     /translation="MTFIFMKVVIDMGSQSLRSFTFSSKKDFLEGKIRSEICPLAKDL
                     EGGFLKESRKEEALILLEDFIKGFAAEDVHLYATSAIREAKDGQAFIDEIKERFKIQA
                     KIISGEEEANLGYEGVKLLAGTSPFSLLDLGGGSTEIVTPHIKKSYPMGVVRYFEKID
                     LGEYYRNLPQIQGKLYAIGGSLSVFVSLSLGSRTYNRERIHGKTIKRSQIKEITNNLA
                     KMSIEERKSHLGTFSKRVYTIIPAGIILDYLLEKLDQKELIYSDFTAIEGYALSRKLI
                     "
     gene            complement(3341..3619)
                     /locus_tag="VFD08_04270"
     CDS             complement(3341..3619)
                     /locus_tag="VFD08_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZK10268.1"
                     /translation="MKTTQRRKRSIIIIALLLILLGLYTFVFQQMEMKKLKEDRVSLQ
                     LEVQRLELLKAKLEEELRRSGSLAYVEDLARGQLKMVMPDEMLYVIHD"
     gene            complement(3616..3861)
                     /locus_tag="VFD08_04275"
     CDS             complement(3616..3861)
                     /locus_tag="VFD08_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZK10269.1"
                     /translation="MRIDKYLKVSRIIKRRTIAQEFCRAGKVKINGKIAKPSERLELG
                     DMLTIQFGRKIVEFEVLELRDSSTKDNAAQMYREVIS"
     gene            complement(3900..4187)
                     /locus_tag="VFD08_04280"
     CDS             complement(3900..4187)
                     /locus_tag="VFD08_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZK10270.1"
                     /translation="MNKTELVAKVAEKTGLTKKASQECIEAILDSIEEALVSGEKVQL
                     VGFGTFEVRDRKARQGRNPQQPEQVIQIPASKAPVFKAGKGLKEAVNQKTK"
     gene            complement(4313..5545)
                     /locus_tag="VFD08_04285"
     CDS             complement(4313..5545)
                     /locus_tag="VFD08_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HZK10271.1"
                     /translation="MLTIVGLGPGGLDQLSLGAYRALEEADKIILRTAWHPAAQELHL
                     PYESLDRLYDEADDFDSLYKEIASYIRQEAQTKNLVYAVPGSPFVAEKTVELLRDLKP
                     KIYPGASFLDVVCAALGIDFSKGLLIEDGLEDFTMLASVDLVLVQVYKKEVASRVKLR
                     LMEVYDDNHPVTIIKYAGTPHEQVHSLKLYELDHFDKFDHLTTLYIPKDNQEARGLKE
                     LYELVERLLAPGGCPWDRAQTHQSLKGYLLEESYEVMEAIDQEDFSALMDELGDLLFQ
                     VVFHASLAQEQGYFTLDDVIEGSYNKIYSRHTHVFSSDQAQKAEDVLDIWEKNKSKEQ
                     SLEKRIASIPKNSPLHYAQKLKKLLGRKKESLKSNEELLDLLETIIEIAVERDLSLDM
                     ALMDRMKLVVEKHSIHKG"
     gene            complement(5545..6096)
                     /locus_tag="VFD08_04290"
     CDS             complement(5545..6096)
                     /locus_tag="VFD08_04290"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZK10272.1"
                     /translation="MDKDYMLNRIATSQLVGEFVEKLNKDYEASDGFIEAQKTLNLVR
                     ASHILLETEEEAQEVKALIEEEPSRFEMLAAERSTCPSAENEGDLGYFESEAMVAEFS
                     QAAFAMEIDEISDIVESQFGYHIIKVTDKGTLFELTENDLEDPSLEETKQRLIEAAVW
                     EEFYRLYEEQVVKTPIQYYSLEN"
     gene            complement(6187..6570)
                     /locus_tag="VFD08_04295"
     CDS             complement(6187..6570)
                     /locus_tag="VFD08_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10273.1"
                     /translation="MNKKRKLLLGILILVLLTTVACSKEENPAEVPVEEENQELEEAE
                     EAEEGASLPTHEEFVSEVDGKGVTKEEYEQYYLYRKYAYIMSYGEEVFEGEEAEKLEE
                     NLRLELQGEIAKEQIFVLLAENAGF"
     gene            complement(6567..9782)
                     /gene="mfd"
                     /locus_tag="VFD08_04300"
     CDS             complement(6567..9782)
                     /gene="mfd"
                     /locus_tag="VFD08_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZK10274.1"
                     /translation="MQLSKLLPTFPKDQKFQLDLVSESLDSLFLSRALDKKALLVVAD
                     LLQAQRFYEELSDLGERVYLLPEKENLRPGVFYSSEELLLERSAVLKEFDKGILITSV
                     KGLGHGLPSRENFQKKELIFTLESQIGRDQLLDYLVQMGYERKEITERWGDFSVRGDI
                     CDVFVPGEEYPFRMEFFGDEIDGMRSFEPQSQRSKENLMEVRVFPALEEKLSKDAYLE
                     DWVEDLFIYDELTCINRYKDYYKDTLLQGENGGEIDLPRGPEVLLDLFEKSRLVVNPH
                     HQPLSENRLLVSVESRRNLDYRGRLGDFFEDLRKRKEYKQILLLSSKDSPLLSELKKE
                     NIVYEIAPDKPQEERLSIYFSSLSKGFVYQKKGLVLYTQAELLGGVKKKRQAHFQEDT
                     LSISSFMDLKIGDYVVHLHHGIGRYGGVVQKQIEGQTRDYLEISYRGSDVLYIPSDQL
                     AMIQRYLGSDARKVSLSKLGSQDWKRKKERAKKSIEDLTEQLLELYAKRESARGYAFS
                     EDTPWQQQFEMDFIYEETPGQLKAIEAVKRDMESPRPMDRLLLGDVGFGKTEVALRGI
                     FKAAMESKQSVFLVPTTVLCQQHYETMKARFSDYPIKIAALSRLTVPREKEEILRGLA
                     QGTIDVVVGTHMVFGKQVQFKDLGLFIIDEEQRFGVVDKEKIRKLRTQVDTLSMSATP
                     IPRTLHLSLGGIRDLSLLREAPKNRFPIQTYIARRDDSLIREAILRELERGGQIYYIF
                     NRISGMEEVKTRLRKFFPQMVIASAHGKMKRQALEETMLDFRRGEIDLLLSTTIVETG
                     MDISNVNTLIVEDAQNFGLSQLYQLRGRVGRSDRLAYAYLLYPAQGELSEEARNRLDT
                     LREFTEFGSGFKIALRDMEIRGSGSILGSDQSGHFADIGYELYMEMLKEKASELKGQK
                     VEVKKRAQLQYNSSAYIPSKYISAEETKMEIYRKINQIENREDLADLQEEITDRFGTM
                     PEVVDELLTISYLSSLAGRMGFSEVILQKGLKLYFDESHPPSVEWVQRLHKLSPKGVQ
                     IRVGVRPFLFIEKEELTIEKAVKALESLDKLGQDASPKEEM"
     gene            complement(9782..10345)
                     /gene="pth"
                     /locus_tag="VFD08_04305"
     CDS             complement(9782..10345)
                     /gene="pth"
                     /locus_tag="VFD08_04305"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZK10275.1"
                     /translation="MKVILGLGNPGNKYAKTRHNVGYQTIEGLAKRLGFSFNKAKFHA
                     LIAEGQVGEEKILLAKPTSFMNLSGIAAAEILNFYKLQPQDLLVIYDDVDLEIGDLRL
                     RKSGGAGTHNGMKDIISRIGSKDFPRLRLGIGQDPRIPLASFVLSRFSDKEEVLMDQT
                     MEEALDALEVFLDKGMDAAMNSYNRRS"
     gene            complement(10346..11305)
                     /locus_tag="VFD08_04310"
     CDS             complement(10346..11305)
                     /locus_tag="VFD08_04310"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZK10276.1"
                     /translation="MHKTGDVVKIFAGNAGKNLSLKICKELGLPLGEAKVGSFSDGET
                     AISIGESVRGADVFIIQSTAPPQVNTNLMELLIMIDALKRASAGRITAVIPYYGYARQ
                     DRKARARDPISAKLVADLVTTAGADRVLTMDLHASQIQGYFNIPLDHLLGQSLLADYV
                     RRKNLEDMVVVSPDFGSVTRARDFSSKIDSTIAIIEKRRPKANEAEVMSIIGEVKGKN
                     VVLVDDMIDTAGTICQAGDVLLDMGAKSVMACCTHAVFSGPAIERLVNSSIDEVVILD
                     TIELEAKKRFEKLTILSSAKIFAEAIRRIYQNDSVSELFNGNK"
     gene            complement(11305..12654)
                     /gene="glmU"
                     /locus_tag="VFD08_04315"
     CDS             complement(11305..12654)
                     /gene="glmU"
                     /locus_tag="VFD08_04315"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZK10277.1"
                     /translation="MDVVILAAGKSTRMKSKTSKVLHKILGKPILSYVVELAEEIKAN
                     KSIVVIAPGNEEIPALYGDRLLYATQWDQKGTGDALKAAVGKISSDTTLVLYGDAPFL
                     RPESLEGFIEFHKGKDLSILSVCQENPKGYGRIIRGEKGVEGIVEEKDCNPEQRKIKE
                     INSGILLMNSELMKENLQKITTSNSQGEYYLTDLVELFYAQGKIVDAYVIDDPEETAG
                     VNDRAQLARGGKVFQKRIIEEHLKEGVSIIDPDRVYIEPEVEIAPDVEIWPDTYLLGK
                     TKIEEDVRLGPGVHLEDSHIMRGAVLEHTRGIQAQVGCDSRVGPYAYLRPGTKIGAGC
                     RIGDFVEIKNSTIGDGTKVSHLSYIGDADLGENINVGCGTIFVNYDGVNKYRSTVGDG
                     AFLGCNSNLISPVEIGANAFVAAGTTVDQKVPENALSIGRVRSEIKLDWNKARNRRK"
     gene            complement(12747..13016)
                     /gene="spoVG"
                     /locus_tag="VFD08_04320"
     CDS             complement(12747..13016)
                     /gene="spoVG"
                     /locus_tag="VFD08_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HZK10278.1"
                     /translation="MLNITDIRIKKVEGGGKMKAIASITLDDAFVIHDIKIVEGYNGV
                     FVAMPSRRLPDGDFRDIAHPINADSRAFLQKKIFSAYEGLEEIKK"
BASE COUNT         3515 a         3025 c         2229 g         4370 t
ORIGIN      
        1 ccttggttgt agcttcttct aatatctcca tagtaatttc aggctgcttc tttcttgcgg
       61 taataagagc agcttcattc atcatatttt ctaagtctgc tggggtaaaa cctggagtcc
      121 ttttagcgat aactccaaaa tctacttctt cattaagagg tttgtccttg gagtgaacct
      181 taaggacagc ctctctttct ttgacatcag gaacaccaac atagacctgt ctatcaaatc
      241 ggccaggtcg taagagggct gggtccagaa tatcgggcct atttgtggcc gcaataataa
      301 tgatcccctc ttgatcacca aaaccatcca tctcaactaa aagttggttt agggtctgct
      361 ctctttcatc atttcccccg cctaggcccg caccacggtg tcgacctacg gcgtcaatct
      421 catcaataaa aactatacaa ggtgtgtctt ttcttgcggt attgaaaaga tctcttaccc
      481 gggaggcacc aaccccaaca aacatttcta caaaatccga accacttata ctgtagaagg
      541 gcacattggc ctctcctgca atggctcttg ataggtaggt ttttcccgtt ccgggaagac
      601 cactcattaa aactcccttg gggatccttg ctcctaattt tctataacgg tcgggacttt
      661 ttaaaaagtc cacaatctcc atcatttctt ctttttcttc atgtagacca gctacatcgc
      721 taaaggtaat tctcctgccg gtatcttgct tgtgcagctt ggcacggttc ttaccaaaac
      781 tcatagcctt atttgctcca ccttgactag agttcataag cataaaccaa gcaaataaca
      841 taatccccat tataagtaaa gtgggtaaga tgctaaccca aaacggctct acatcaggcg
      901 ctgctgtttc aataggaata tcctggtttc ttgccgcatc taaaacttcc gttccataat
      961 actcagcaaa cattccagga acaaagacgg caaattccgt tccgtccttt aaaaaaccac
     1021 caaccacgtt gtctttaacg ctcatagact caacttggtt cgtttcaatt ttcataatta
     1081 catcggtaaa gtttaactca actgctgttt cggttggttg tagggaactc caaaaaacaa
     1141 aaaccaaaac agtcagtata agtatccaaa aaaacatatt tttttgcatt tttttcaagt
     1201 cactgcctcc ttaatacacc aatctatgtt acaccaaatg atcttatttt tcaataaata
     1261 tttgaagata attttgagcg gtagtcgaaa tttgggcctt tctaagtcca ataatccaaa
     1321 aaaccgtctc tgctgaagct aagagccaat atttgtctct ttgtgaacgg ggtatcttct
     1381 catctataaa ataatctttt aatttctttt taccttgaag gccacttggt tgaaacatat
     1441 ctccgggtct tcggtggcga agaactaatg gttcttctaa aagttcttca ggaatactat
     1501 gagtgttttc cgtaaaaagc ggcttctgag cacttctaat ttcaaaggta taaccctcaa
     1561 gttcattagt acccaaaaga agcttgtggc taacactagg tttttttata tagggaccta
     1621 tgtaaaaaaa atcttgctcc ctgcgaagga gtccctcaga aaaatgtttt tctcccctag
     1681 atcgttcttc taggatatcc atagcctcct gggtttgtgc atagtttagt gaaaactttc
     1741 ttctaatgaa ggcttttttt aagactggat gcagctggcg tatttcttct accccataaa
     1801 gctctttatc ccaatcgatt ttttcatcaa gcaattcatt tataaaggca ttttcttcgc
     1861 gaagaatttc cattgaagta agcagtcttt tagaaaggtt tggggcatac tcttcttcaa
     1921 ttttaggaat taagtctagt cggaggcggt ttctactata aatcggctct aaattagttt
     1981 ggtctattac ataggctaac ttgttcttct tggcatattc catgatttct tctttacgaa
     2041 aagaaagaag aggccgatag agatcccctt ttactgggtg aatcccgcca aggcctgctg
     2101 tgccacttcc tctaaaaagg ttcataaaaa aagtttccat ttgatcatct agatgatggg
     2161 ctgttgcaat ataatcaaga tttctttctt tttttatttt cctaagaaaa tcatagcgaa
     2221 gatttcttgc tccttcttca attcctagtc ctttttctct acagtattct tctacatcca
     2281 aactatagct atgaagaagt agcttttttt ctttggccaa attggcacaa aatctagcgt
     2341 catcttttga attttctctt aggttgtgat ctaagtgaaa aagttcaact tcacagcctt
     2401 cctttaaaaa aaggtccaaa agaacgacag agtcaggacc tccgctaaag gctagcaata
     2461 tttttccttt tagttgcgga attagtttca tatcaacttc ctagataggg catatccttc
     2521 tatcgcagta aaatcagaat aaataagttc tttctggtct agcttctcaa gaagataatc
     2581 cagtattata cctgctggta taatagtata tactcttttg ctgaaagtgc ccaaatggct
     2641 ttttctttct tctatactca ttttagctaa attgtttgtt atttctttta tttggcttct
     2701 ttttattgtt ttgccgtgaa ttctttctcg attgtaggtc ctactcccta aagaaagact
     2761 gacaaaaaca cttagactgc cccctatggc atataatttt ccctgaattt ggggcaggtt
     2821 tctataatac tcccctaaat caatcttctc aaaataacgg acaacaccca tgggatagct
     2881 ctttttgata tggggcgtca caatttctgt agaaccgccc cctagatcta ggagggaaaa
     2941 aggggatgtt cctgctaaga gctttactcc ctcatagcca agattggctt cctcttcccc
     3001 tgagataatt tttgcctgta tcttaaaccg ttcttttatt tcatctataa aagcttggcc
     3061 atctttggcc tcgcgaattg cacttgttgc atagagatgg acatcttcag cagcaaaacc
     3121 ctttataaag tcttctaata agatcaaggc ttcttctttt ctgctttctt ttaaaaagcc
     3181 cccctcaaga tcctttgcta aggggcatat ttcactgcgt attttcccct ctaaaaaatc
     3241 ttttttcgaa ctaaaggtaa aagacctaag ggactggctt cccatatcaa ttactacttt
     3301 cataaaaata aaagtcaaga tttctcttga cttttccccc tcaatcatgg atcacataaa
     3361 gcatttcatc gggcattacc atttttagtt gccctcgtgc tagatcttca acataagcta
     3421 aagaacctga acgacgtaac tcttcttcga gtttggcctt taaaagttct aaacgttgga
     3481 cttccaattg cagagaaact cggtcttctt ttaacttctt catctccatt tgctgaaaaa
     3541 caaaagtata aagtcctaag agaatcagta atagggctat gataataatg gagcgttttc
     3601 ttctctgtgt cgttttcatg aaatcacttc cctatacatc tgagccgcat tatctttagt
     3661 ggaactatcg cgtagttcca agacttcgaa ttctacaatt ttccttccaa actggattgt
     3721 aagcatatct cctaattcta atcgttctga gggcttagca atctttccat ttatttttac
     3781 ttttccggca cgacaaaatt cttgggcaat ggtgcgacgc ttaatgatcc ttgagacctt
     3841 tagatattta tctatgcgca tctttttgcc ttgttaaata aagagtagct atgctactct
     3901 tattttgtct tttggtttac tgcttctttg aggccttttc ctgctttgaa aacaggtgcc
     3961 tttgaagcgg gaatttgaat tacttgttct ggttgttgtg ggtttcttcc ttgacgagct
     4021 ttacgatcac gcacttcaaa agtgccaaat ccaactaatt gtactttttc accacttact
     4081 aatgcttctt caatactatc taaaatagct tcgatacact cttgtgatgc tttctttgta
     4141 agacctgttt tttctgctac ttttgctact aattcagttt tattcattct ttcctccttg
     4201 tgtaacttaa atctatgata cccgcaaaac cgctaaaata caagctcttt tttcgttttt
     4261 gctaggtttt cctgtaattt tcacttcatt taaaaatatt ttttcttaga tactatccct
     4321 tgtgtatgga atgtttttct accacaagct tcattcgatc catcagggcc atatcaagag
     4381 ataggtctct ttccacagca atttcaataa ttgtttccag caaatccaaa agttcttcat
     4441 tactttttag tgattctttt ttcctaccta ggagcttttt aagtttttgt gcataatgca
     4501 agggactgtt ttttggaatc gatgcaattc ttttttccag gctctgctct ttgcttttat
     4561 ttttctccca gatatctaaa acatcctctg ccttttgggc ctgatcagaa ctaaagacat
     4621 gggtgtgacg cgaatagatt ttattatagg agccttcaat aacatcatct agggtgaaat
     4681 agccttgctc ttgggctagt gacgcgtgaa agacaacttg aaataagagg tcccccagtt
     4741 catccataag ggcactaaag tcctcttgat caatcgcttc catgacttca tagctttctt
     4801 ccagaagata gcctttgaga ctttggtggg tctgagctct atcccaaggg catccacccg
     4861 gagctagtag cctttctacc agttcatata actcttttaa tcccctggct tcttgattgt
     4921 cttttggaat atagagtgtt gttaaatgat cgaatttatc aaaatgatcc agctcatata
     4981 gcttaagact gtggacttgc tcatggggag tccctgcata ttttataatg gtgactggat
     5041 gattgtcatc atatacctcc ataaggcgga gctttaccct tgaagctact tcctttttat
     5101 atacctggac caagactaga tctacagaag ctagcatagt aaagtcttct agaccatctt
     5161 caattaatag tcccttggaa aaatctatgc ctagggcagc gcatactaca tctagaaagc
     5221 ttgcgcctgg atagattttt ggcttcaagt ctctaagtag ctctactgtt ttttctgcca
     5281 caaaggggga accaggcact gcgtaaacaa gattttttgt ctgggcctct tgtctaatat
     5341 agcttgctat ctccttgtat agactatcaa aatcatctgc ttcatcataa agacgatcca
     5401 agctctcata gggcagatgc agctcctgtg ctgcaggatg ccatgctgtt cgtagaatga
     5461 ttttatccgc ttcctctagg gccctataag cccctagact aagctgatcg agtcctccag
     5521 ggccaaggcc tacaatagta agcattaatt ttctagggag taatactgaa tgggggtttt
     5581 aaccacctgc tcctcataga gtctataaaa ctcttcccat acagcggctt caataaggcg
     5641 ttgttttgtt tcttctaagc ttggatcctc aagatcattt tctgttagtt caaatagggt
     5701 ccctttgtct gttactttaa taatatgata gccaaactga ctttcaacga tatccgatat
     5761 ttcgtcgatc tccatagcaa aggctgcctg ggaaaactcc gctaccatgg cttcagattc
     5821 aaaatatcct aaatctcctt cattttctgc actagggcaa gtagatcttt ctgctgctag
     5881 catttcaaag cgggaaggtt cttcttcaat taatgcctta acctcttggg cttcttcttc
     5941 tgtttctagt aagatatgac tggccctgac aagatttaag gttttttgtg cttcaataaa
     6001 gccatcgctt gcctcatagt ccttattgag tttttctaca aactcaccta ctaattgact
     6061 tgtggctatg cgattgagca tatagtcttt atccaagcca ttttcttcaa agtatttaag
     6121 agtttcctcg gtatttgtaa caagaaaatc ctctacatat attgttgctg cttcttctaa
     6181 atctttctag aaaccggcat tttctgccag caaaacaaag atttgctctt ttgctatttc
     6241 tccttgaagt tctaggcgta agttttcctc taatttttct gcttcttcgc cctcaaaaac
     6301 ttcttctcca taactcataa tataagcata ttttctataa agataatatt gttcatattc
     6361 ttcttttgta acgcccttgc catctacctc agaaacaaac tcttcatgag taggcaggga
     6421 tgcgccttct tctgcttctt ctgcttcttc aagctcttgg ttttcttcct ctacaggaac
     6481 ttcagctgga ttttcttcct tgctacaggc tactgtagtt agcaaaacca gaattaaaat
     6541 tcctaagagt agttttcgtt ttttattcac atttcctcct taggtgatgc atcttgtcca
     6601 agtttgtcta gactctctaa tgccttcacc gccttttcta ttgttaactc ttccttttca
     6661 ataaagagaa agggtcgaac gcccactctt atctgtaccc ctttaggtga gagtttatgc
     6721 aagcgctgca cccattctac actaggagga tggctttcgt caaagtagag ttttagtcct
     6781 ttttgtaaaa ttacctcact aaagcccatc cttccggcta gggatgatag ataactaatg
     6841 gtaagaagct catccaccac ctcgggcatg gtgccaaagc ggtctgttat ttcttcttgt
     6901 aaatcagcca gatcttcccg gttttcaatt tggttgattt ttcggtagat ctccattttt
     6961 gtttcttctg cagaaatata cttggatggg atataggccg agctattata ctgaagctgg
     7021 gctctttttt tcacttccac cttttgtcct ttaagttcac ttgccttttc ttttagcatt
     7081 tccatataga gttcgtatcc aatatctgca aaatgccctg actgatccga ccctaatata
     7141 ctgccagaac cccttatctc catatcccgc agagcgattt taaagccaga accaaattcc
     7201 gtaaactccc ttaaggtatc cagtctattt cttgcttcct cactcaattc tccctgggct
     7261 ggataaagaa gataggcata ggctaaacga tcgctccttc ccaccctacc tcgcagctga
     7321 taaagctggg ataagccaaa gttttgagca tcttctacaa tcagtgtatt tacatttgaa
     7381 atatccatcc ctgtttcaac aatggtggta gaaagaagaa gatctatttc tcctcttcta
     7441 aaatcaagca tcgtctcttc taaagcctgt cgcttcattt ttccatgggc cgaggcaata
     7501 accatttgcg gaaaaaactt tcttagtctt gtttttacct cttccatacc agaaatccga
     7561 ttaaaaatat aatagatttg tcccccgcgt tctagctctc taagaattgc ttctcgaatc
     7621 agactatcat ctcttctagc aatataggtt tgaataggaa agcggttctt tggagcctca
     7681 cgaagaaggc ttaaatctct aatccccccc agtgaaagat gaagtgtacg gggtataggg
     7741 gttgctgaca tactcagtgt atcaacctgg gtgcgcagct tgcgtatttt ttccttatca
     7801 acaacaccaa agcgctgctc ctcgtctatg ataaataagc ccaaatcttt aaattggacc
     7861 tgcttaccaa ataccatatg agtgcccacc acaacatcaa tagttccttg ggcaagaccc
     7921 cttaatattt cttccttttc tctagggact gtcagccttg aaagggcagc tatctttatg
     7981 ggataatcac taaagcgtgc tttcatggtt tcatagtgtt gctggcaaag aacggttgtt
     8041 gggacaagaa agaccgactg tttgctttcc atggcagctt taaaaatccc ccttagggct
     8101 acctctgttt ttccaaaacc tacatcacca agaagaagcc ggtccatagg ccttggggac
     8161 tccatatctc tttttaccgc ttcaatggcc tttagctgac ccggggtttc ttcatagata
     8221 aaatccattt caaactgctg ctgccaaggg gtgtcctctg aaaaagcata gcccctagca
     8281 gattcccttt tagcatagag ttctaagagc tgctctgtta gatcttcaat gctctttttg
     8341 gctctttctt tttttctttt ccagtcctga cttcctagtt tacttaagga gacttttctt
     8401 gcatcactgc ccaaataacg ctggatcata gctagctgat cagagggaat ataaagaaca
     8461 tcacttccac gataggaaat ttcaagataa tctcgggtct ggccctcaat ctgcttttga
     8521 accacaccgc catagcggcc aattccatgg tggagatgga ccacatagtc tcctatttta
     8581 aggtccataa aggagctaat agaaagtgtg tcttcttgaa aatgagcctg ccttttcttt
     8641 tttactccac ccaaaagttc agcctgggta tatagtacaa ggcctttttt ctggtagaca
     8701 aagcccttgc ttaaggaaga aaaatagatg cttagccttt cttcttgggg cttatcaggg
     8761 gcgatttcat agacaatatt ttcctttttt aattcactta gaaggggact gtctttagaa
     8821 gaaagaagta atatttgctt atactctttt ctttttctta ggtcctcaaa aaagtcgccc
     8881 agcctgcccc tataatcaag gtttcttctg gattcaacag atacaagaag tcgattttct
     8941 gataggggct ggtggtgggg attgacaaca agtctgcttt tttcaaagag gtctagaagg
     9001 acttctggtc cacggggcag atcaatttcg ccaccatttt ccccttgcag taaagtgtct
     9061 ttataatagt ccttataacg atttatacag gtaagttcat cataaataaa gagatcttcc
     9121 acccagtctt ccaaataagc gtcctttgaa agcttttctt ctaaagcagg aaaaaccctt
     9181 acttccatca agttttcctt gcttctttgg ctttggggct caaagctacg catcccatca
     9241 atctcatcac caaaaaactc cattcgaaaa ggatattctt ctcctggaac aaaaacgtca
     9301 cagatgtctc cccgtactga aaaatcgccc catctttccg taatttcttt tctttcataa
     9361 cccatttgca caagataatc caaaagctgg tctcttccta tttggctttc tagggtaaag
     9421 attagctctt ttttttgaaa gttttctctc gagggtaggc catgtccaag ccccttaaca
     9481 ctggtaataa ggatgccctt atcaaactct tttaaaacag cacttctttc taaaagcaat
     9541 tcttcagagg agtaaaaaac accaggtcgc aagttttctt tttctggaag aagataaact
     9601 ctttctccca agtcacttaa ctcttcataa aagcgttggg cctgtaaaag atctgccacc
     9661 acaagtaggg cctttttgtc taaggcccgg cttaaaaaga gtgaatccaa ggattcactc
     9721 actaggtcaa gttgaaactt ttgatctttt ggaaaagttg gcagcaattt tgacaattgc
     9781 attagcttct cctattgtag ctgttcatgg cagcgtccat tcctttgtca agaaaaactt
     9841 ccaaggcgtc aagggcttct tccattgtct ggtccataag aacttcttct ttatcggaga
     9901 atcttgatag gacaaaacta gcaagaggta ttcttggatc ttgtccaatc cccaacctta
     9961 atctgggaaa atccttgctc ccaatacggc taataatgtc tttcattcca ttgtgagtac
    10021 ccgcaccacc gcttttacga aggcgcaagt ctccaatctc caagtctaca tcatcataaa
    10081 ttacaagaag atcctgtggc tggagtttgt aaaaatttaa aatttctgca gcagcaatcc
    10141 cacttagatt cataaaactt gtaggctttg caagaaggat tttctcctcc cccacctgcc
    10201 cctctgctat aagagcgtga aatttagctt tattaaagga aaatcctagc cttttagcaa
    10261 gaccttctat tgtttgatat cctacattat ggcgtgtctt ggcatattta ttcccaggat
    10321 ttcccaaacc tagaataacc ttcatttatt tatttccgtt aaatagctct gaaacagaat
    10381 cattttgata aatacgacgg atggcctcag caaagatttt tgcactgctt aaaatagtca
    10441 gcttttcaaa gcgttttttc gcctccagtt cgatggtatc taaaataact acttcatcaa
    10501 tagaactatt cacaaggcgc tcaatagctg gcccactaaa gaccgcatgg gtacaacagg
    10561 ccataacact ctttgctccc atatctaaaa gcacatcccc tgcctgacaa atcgttcctg
    10621 cggtatcaat catatcgtcg acaagaacaa catttttccc cttgacttcc cctataatac
    10681 tcataacctc agcctcatta gccttgggtc ttcttttttc aataatagca atggtgctat
    10741 caatttttga ggaaaagtcc ctagctcggg ttacagatcc aaaatcagga gataccacga
    10801 ccatatcttc caggtttttt ctgcgcacat aatccgctaa aagagattgg cccagtagat
    10861 ggtctagagg aatattaaag tatccttgaa tttgagatgc gtggaggtcc attgttaaaa
    10921 ctctgtcggc accagctgtt gtaactaaat ctgctacaag ttttgcacta atagggtctc
    10981 ttgctctagc tttacgatct tgcctggcat aaccatagta gggaataaca gcagtgatcc
    11041 tacctgcaga agcacgctta agggcgtcaa tcataattaa aagttccatt aagttggtat
    11101 taacctgggg aggggcggta gattgaataa taaaaacatc cgctccacga acactctcac
    11161 caattgaaat cgctgtctca ccatcgctaa agctacctac ttttgcttcg ccaaggggca
    11221 aacctaactc tttacaaatt tttagggaaa gatttttacc cgcatttcct gcaaaaatct
    11281 ttacaacatc ccctgtttta tgcattattt cctcctgttt ctagctttat tccaatcaag
    11341 ttttatctct gagcgtactc gtccaatact aagagcattt tctgggactt tttggtctac
    11401 tgtggttcct gctgcaacaa aggcatttgc cccaatttct acaggagaaa ttaaattact
    11461 attgcacccc agaaaagcac catcgcccac cgtcgatctg tacttgttca ccccatcata
    11521 attgacaaag attgttccac aacctacatt tatattttcc cctaaatccg catcaccaat
    11581 atagctaaga tgacttacct tggttccatc accaatggtg gagttcttaa tttcaacaaa
    11641 gtcacctata cggcatcctg caccaatttt cgtgcctgga cgcaaatagg cataggggcc
    11701 aacccgacta tcacagccta cctgggcctg gattcccctt gtatgctcca gaactgcccc
    11761 cctcataata tgtgaatctt ccaaatgaac gcctggtcct aggcggacgt cttcttctat
    11821 cttggttttt cctaatagat aggtatctgg ccaaatctca acatctggag caatttccac
    11881 ttctggctcg atatagaccc gatcaggatc aattatgcta acaccctctt ttaggtgttc
    11941 ttctatgatc cttttctgaa agacttttcc tcccctggct aactgggctc tatcatttac
    12001 tcctgcggtt tcttcaggat cgtcaattac ataggcatca actattttcc cctgggcata
    12061 aaagagttcc actagatcag taagatagta ttccccctgg gaattgctag tggtaatctt
    12121 ttgtaagttt tccttcatca actctgaatt cataagaaga attccgctat taatttcctt
    12181 gattttcctt tgttctgggt tacaatcctt ttcttctaca atgccctcta ctcccttttc
    12241 tcctcgaata attcttccat atcccttggg gttttcttgg caaacactaa ggatacttag
    12301 gtcctttccc ttgtgaaatt caataaaacc ctcaagactt tctgggcgta aaaagggtgc
    12361 atccccataa agtaccaggg tggtatcaga actaatttta cccacagcag ccttaagggc
    12421 atcaccagtt cctttttgat cccactgggt ggcatagagt agacgatcac catacagggc
    12481 cggtatttct tcgtttcctg gagcgattac tacaatgctt ttatttgctt ttatttcttc
    12541 tgctaattcc accacataac ttagaatcgg ttttccaaga attttatgta gaaccttgct
    12601 tgtttttgat ttcattcggg tgcttttacc agcagccaaa attacaacat ccataaagta
    12661 cccccattta ttattataca tcagcttatg tgtttttctc aaagcttctt cttttataaa
    12721 gacaaaaaga gaagctacac ttctctctat ttctttattt cttccaagcc ttcataggca
    12781 gaaaagattt ttttttgtaa aaatgccctg ctatctgcgt tgataggatg ggctatatcg
    12841 cgaaagtctc catcaggcaa tctcctgcta ggcatggcca caaaaacacc attatatcct
    12901 tccacgattt taatatcatg aattacaaaa gcgtcatcta gggtaatgga ggcaatagct
    12961 ttcattttcc ccccaccttc cacttttttt atgcgtatat cagtgatatt tagcatgtct
    13021 tttcctttct actttctagc tttactttct ccataaatca gcccgatatt aacatatctc
    13081 tatttatttt tcaatcttac taaaagcctg agctttttct ttttggataa agcttcttc
//
LOCUS       DASRNA010000157         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_947616,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000157 DASRNA010000000
VERSION     DASRNA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947616"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2086
                     /gene="pgmB"
                     /locus_tag="VFD08_04325"
     CDS             <1..2086
                     /gene="pgmB"
                     /locus_tag="VFD08_04325"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HZK10279.1"
                     /translation="QDSIRKNKTDFISKEELYEKQKEYLRDYNKRCRIDFLDNKELDT
                     AMKFMRFHLLQATTRDSYGNISAKGLSGEGYEGHYFWDTEIFLFPVWLLWDKKRAKNL
                     LLYRYGLLDIARARAKEMGNSRGACFSWRTISGIESSGYFPAGSAQYHINFDIAYTFI
                     QWWLSNYDWDFLITYSMEVLIETARTALEIGSFEKGKFHIHGVTGPDEYTALVSDNYY
                     TNKMAQYNLSWAVKLWHKLEKERWDDWIKLKEKLSVSQGEIEKMQEAAQNMVFIYDKE
                     KGIRVQDASFLQKAKWPKENKKRPLLLHYHPLNIYRHQIIKQADTVLGQYLLGEENEE
                     IMRKSFYYYEEINTHDSSLSRSISGLMAARLRDEKKAYDYFMDSVYLDIEDLQENTRD
                     GLHMANIGGSLLSVLRGFGGLTIEEEGLYINPFLPKKLGRLSFRFTWREALLEVLLEG
                     EEVDIRKISGPKREIILRGEKIRLGEKAVLFDLDGVLTGTSDNHYEAWKKMSKELAYD
                     LPENFRDKLRGIGRMESIDLILEDFGLSYSLEEKIRLTDRKNDYYKESIRHFGQDNLY
                     PGVLELLRELRKRKVKIGLVSASANAKALIKSMEIEKYFDEVVDPRQIKKGKPHPDPF
                     LAAAKGLKVLPKDCLGVEDALAGIESINAAGMTSLGIGGSDLAQADFRYKTIKEASDF
                     IIRWVEEDYGRN"
     gene            2073..>3105
                     /gene="ugpC"
                     /locus_tag="VFD08_04330"
     CDS             2073..>3105
                     /gene="ugpC"
                     /locus_tag="VFD08_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZK10280.1"
                     /translation="MAEISIKNLTKVYDNGFKAVKSIDLDIKDQEFMVLVGPSGCGKS
                     TVLRMIAGLEEITSGELRIGGERMNEVDPRDRNIAMVFQNYALYPHMSVYDNMAFGLK
                     LKKLDKAEIKKRIEEAARALDIENQLSKKPKLLSGGQRQRVALGRAIVRNPRVFLMDE
                     PLSNLDAKLRVQMRAELIKLHEELKTTFVYVTHDQTEAMTMGSRICVLKDGEIQQVAT
                     PEEIYNKPDNLFVASFIGSPQMNFYKGELIKEGGDYFLAGKNKKYGDFKFPLTKAPEE
                     KLRPYIDKEVILGARPEHMTISNDQGLKAEVMVTELLGSEIHVHVYVQDEVHTAKVEA
                     PTKIRAGDVI"
BASE COUNT         1053 a          496 c          749 g          807 t
ORIGIN      
        1 ccaagattct attagaaaaa ataaaacaga ctttatttca aaagaagagt tatatgaaaa
       61 gcaaaaagaa tatctaaggg attataataa gcgctgcaga atagactttt tagacaataa
      121 agagctggat acggccatga aatttatgcg ttttcatctg cttcaagcca ccactagaga
      181 tagctatgga aacatttcag ccaagggcct ttctggtgag ggctatgagg ggcactattt
      241 ttgggataca gagatatttt tatttcctgt ttggctctta tgggataaaa aaagagccaa
      301 aaacctccta ctctatagat atggcctgct tgatattgcc agggccagag caaaggagat
      361 gggaaatagc aggggcgcct gcttttcctg gaggaccata tctgggatag aatcatcagg
      421 ctacttcccg gcgggttctg cccagtacca tattaacttt gatattgcct atacctttat
      481 tcagtggtgg ctttcaaatt acgattggga ttttttaata acttattcta tggaagtact
      541 aatagaaaca gccagaacgg cccttgaaat tggctccttt gaaaagggga aatttcatat
      601 ccatggcgtt acaggtcctg atgagtatac ggccctggta tctgataatt actataccaa
      661 taaaatggcc cagtataatt tatcctgggc agtaaaactc tggcataagc tggaaaaaga
      721 aagatgggat gactggataa aactaaaaga aaagcttagt gttagccaag gagaaatcga
      781 aaaaatgcaa gaagctgccc aaaatatggt ctttatatat gataaagaaa aaggcataag
      841 ggtccaagat gcaagttttc tgcaaaaggc caagtggcca aaggaaaata agaagagacc
      901 tcttctttta cattatcacc cgctgaatat ttatcggcat caaattatta aacaagcaga
      961 taccgtacta ggtcagtatc ttttagggga agaaaatgaa gaaataatga gaaagagctt
     1021 ttattattat gaggaaataa atacccatga ttcttcttta tctagatcca taagtggcct
     1081 tatggccgca agactaagag atgagaaaaa agcctatgac tattttatgg actcggttta
     1141 tctagatata gaagacctgc aggaaaatac cagggatgga cttcatatgg caaatatagg
     1201 agggtccctt ttatcagtac taaggggctt tgggggcctt actatagaag aggaggggct
     1261 ttatataaat ccctttttgc ctaagaagct aggaagacta agctttaggt ttacttggag
     1321 agaggccctg ctagaagttt tacttgaggg ggaagaggtg gatataagga agatctctgg
     1381 gcctaagagg gaaattatac taagggggga aaaaataagg ctgggggaaa aggccgtctt
     1441 atttgatcta gatggggtac taactgggac ttcagataat cattatgaag cttggaaaaa
     1501 aatgagcaag gaactagcct atgatctgcc agaaaacttt cgggataaac ttcggggaat
     1561 aggaagaatg gagtccattg atcttatttt agaagatttt ggccttagct attcattaga
     1621 agaaaagatt agactaactg ataggaaaaa tgactattat aaagaatcaa ttaggcattt
     1681 tggccaggat aatctttatc caggagtgct ggagctctta agagagctca gaaaaaggaa
     1741 ggttaaaatt ggcctggttt ctgccagtgc aaatgccaag gccttaataa agagtatgga
     1801 gatcgaaaag tattttgatg aagttgttga tccccgccaa ataaaaaagg gaaagcccca
     1861 tcccgatcca tttcttgcag cagcaaaggg gctaaaagtt ttgccaaaag attgtttggg
     1921 agtagaagac gccctggcgg gcatagaaag tataaatgca gcgggaatga cctcgctagg
     1981 aattggaggg agcgatctag ctcaggcaga ctttagatat aagacgataa aagaagcttc
     2041 agactttata ataaggtggg tggaggaaga ttatggcaga aattagtatt aaaaacttaa
     2101 ccaaggtata tgataatgga tttaaagctg ttaagtctat tgatctggat ataaaagacc
     2161 aggagtttat ggttttagtt ggtccttcgg gttgtggcaa atccactgta cttagaatga
     2221 ttgctggcct agaagaaata acatctgggg aacttcgtat tgggggcgag cggatgaatg
     2281 aagtagaccc cagggataga aatattgcta tggtttttca aaattatgca ctttatccac
     2341 atatgagtgt ctatgacaat atggcctttg gcttaaagct aaagaaatta gacaaggcag
     2401 agattaaaaa aagaatagag gaggcagcca gggccctgga tatagagaac cagctaagca
     2461 aaaagcccaa actgctttct ggtggacaaa ggcaaagggt ggccctgggc agggccattg
     2521 ttagaaatcc cagagtcttt ttaatggatg agcctctttc caacctggat gcaaagctaa
     2581 gggtacagat gagagctgaa cttataaaac tacatgaaga attaaagaca acctttgtct
     2641 atgttaccca tgatcagaca gaggccatga ccatgggttc tagaatttgt gttttaaaag
     2701 atggagagat acaacaggtg gcaactccag aggagatata taataaacca gataatttat
     2761 ttgtagcaag ttttattggt tctcctcaaa tgaactttta taagggcgag ctgataaagg
     2821 agggggggga ctacttcttg gcgggaaaaa ataaaaaata tggggacttt aaattccccc
     2881 ttacaaaggc tccagaagaa aaactccgtc cctatataga caaggaagtg attctagggg
     2941 ccaggccgga acatatgact ataagtaatg accagggatt aaaggcagag gttatggtta
     3001 cagaacttct tggcagtgag atacatgtac atgtctatgt acaagatgag gtccatactg
     3061 ccaaggtaga ggctccaacc aagataaggg caggggatgt tatag
//
LOCUS       DASRNA010000158         4616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_642993,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000158 DASRNA010000000
VERSION     DASRNA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642993"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFD08_04335"
     CDS             <1..208
                     /locus_tag="VFD08_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZK10281.1"
                     /translation="GVISSHHTHIWSLDSEHHVLSTHLVLDKSLSLDQVIEIKRQARE
                     KIDYLKIEHITIEVDFEDDDCGLQ"
     gene            complement(308..4585)
                     /locus_tag="VFD08_04340"
     CDS             complement(308..4585)
                     /locus_tag="VFD08_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HZK10282.1"
                     /translation="MNSVFNIGLDIGSTTVKILVLDEQDNIVHKEYKRHYSNVREALF
                     TMLSDCHTIIADKKTKINATGSGALDVSKAFDIPFIQEVAAGTTAIQEKIPHTDVAIE
                     LGGEDAKITYLRGQLEQRMNGSCAGGTGAFIDQMAILLETDAQGLNELAKNHNTIYPI
                     ASRCGVFAKTDIQPLLNEGASKENIAASIFQAVVNQTISGLAQGRPIRGNIAFLGGPL
                     TFLSELRERFIDTLALSDKEVIFPKNSEYFVALGAAINAKKAKTMNYGDLLKKVQDRN
                     NQPLQRKKELDPLFKGQKEYDEFIYRHNKARVKRKDINSYRGEAFLGIDAGSTTTKIA
                     LIDKDKNLLHSFYDSNRGNPLQSVISALKNLYEILDNKEIHIVNSTVTGYGEHLVKSA
                     LQVDMGEIETISHYKAADYFSSGLDFVLDIGGQDMKSLKIKNGVIDSVSLNEACSSGC
                     GSFIETFAKSLNTDVKTFAKNSYFSEHPVDLGTRCTVFMNSKVKQAQREGATVADIAA
                     GISISVIKNALFKVIRFNSFDDLGDKIVVQGGTFYNNSVLRAFELLTGKKVIRPDIAG
                     IMGAFGAAIIAKERYTGASSSLLKKEELEAFEISSKMKRCGLCGNNCLLTISKFNDGR
                     YFVSSNRCEKGAELYNSSKEEKTKEEIPNLYAYNYKEVFNYKALPLDKAPRGLIGIPR
                     VLNIYEDYPFWFTFFTKLGYRVVLSGHSNKKIYEMGINTIPSESVCYPAKIAHGHIQD
                     LINKKLRKIFYPCVPYTIIEDKEAGNHFNCPVVTSYPETIHSNFEFDDLGIKFYKPFV
                     NLNDKKLLTKKLFQELREENISFKETKEAVDLGYKELLAYKEKIQKKGQETLDYMKRN
                     KKKGILLAGRPYHLDPEINHGIPEMIQSYGFVVLSETSLSHLAKAPRPLRVIDQWSYH
                     TRLYSAASYVARQKDLELVQLNSFGCGLDAVTSDQVAEILEKNNKIYTLVKIDEINNL
                     GAVRIRIRSLMAAIKERDKNPITVRKRKSEKKRTLFTKKMSEEYTILCPQMSPMHFQF
                     FKAAAAKSGYKVEVLPSVDKQAIDEGLQCVNNDACFPAIITIGQLMEALKSGQYDLDK
                     TALIISQTGGGCRATNYIAFIKKALEDGGFDNTPLISLNAVGLEKHPGFKITPKLIHN
                     LLMGVVYGDVFMKVLYKIRPYERISGSANRLYDYWVDKCQESLINGSFKDFKNNLRNI
                     IRDFDHLQIYEDLKKPQVGVVGEILVKYHPTANNDIVSFLEEEGAEAVVPELLDFFYY
                     ATYNRVIRHKEIIKKDLIGSILNSTAIKILDYYRKEAVSAFKASKRFSPPHNIKDLAS
                     KANRHLSLGNQTGEGWLLTAEMVSLLEEGVNNILCLQPFACLPNHIVGKGIINELKRS
                     YPMANIAPIDYDPGASEVNQINRIKLMLTSAFNDLMPPEKESVPIKP"
BASE COUNT         1301 a          896 c          852 g         1567 t
ORIGIN      
        1 gggggtaatt agtagccatc acactcatat ctggtctcta gacagtgagc atcatgtctt
       61 atccactcat ttggtgcttg ataaaagtct tagtttagac caagtaatag aaatcaaaag
      121 gcaagccaga gaaaaaatag actatttaaa aatcgagcat attacaatag aagtggactt
      181 tgaagatgat gattgtgggc tgcaataaaa agaaatgtaa agctaggacc aaagcatatt
      241 aaaagatagt aaattacaat ccacaaagaa aaagacacag gctcttttat caagagcctg
      301 tgtcttttta tggttttata ggaacacttt ctttttcagg cggcataaga tcattaaaag
      361 cgcttgtaag catcaattta atcctgttta tctggtttac ttcactggct cctggatcat
      421 aatctatggg agcaatattt gccatgggat agcttcgctt taattcattg atgatgccct
      481 ttcctacaat atgattaggt aggcaggcaa agggttgaag gcaaagtata ttgttgactc
      541 cctcttcaag taggctcacc atttcggcag ttaagagcca tccttctcct gtttggtttc
      601 ccaaagaaag atgcctattt gccttactag ctagatcttt aatattatgg ggaggagaaa
      661 aacgtttgct agccttaaag gcacttactg cctctttcct ataatagtct agtattttaa
      721 tggcagtaga atttaaaatg ctccctatta aatccttttt aatgatttct ttgtgtctaa
      781 ttactctatt ataggtagca taataaaaga agtccaaaag ttctggtacg acagcctcag
      841 ctccttcttc ttctaaaaag ctgacaatat cattattggc tgtaggatga tattttacta
      901 ggatttctcc taccacacca acctggggct tttttaaatc ttcataaatt tgcagatgat
      961 caaaatctct aattatattt cttagattgt ttttaaaatc cttaaagctc ccatttatta
     1021 gactttcttg gcatttatcg acccaataat cgtaaagcct attggctgag ccgctaattc
     1081 tttcataggg tctgatttta tagaggacct tcataaagac gtccccatat acgactccca
     1141 taagaagatt gtggataagt ttaggggtta ttttaaaacc gggatgtttt tctaagccca
     1201 ctgcattaag agaaataagg ggagtattgt caaagcctcc gtcctccaaa gcttttttga
     1261 taaaggctat atagttggtg gccctacatc ctcctcctgt ttgagagatt ataagtgcag
     1321 ttttatctag atcatactgg ccagatttta aggcttccat caactgtcct atcgttataa
     1381 ttgcaggaaa gcaggcgtcg ttatttacac attgtagtcc ttcatctatg gcctgcttgt
     1441 ctacagaggg cagtacttct accttgtatc cactcttggc cgctgcagct ttaaagaatt
     1501 gaaaatgcat gggagacatt tgagggcata aaatggtata ttcctcactc atttttttgg
     1561 taaaaagcgt tcttttcttt tcgctttttc tttttcttac tgtaatagga ttcttgtctc
     1621 tttcttttat ggcagccata agagagcgta ttcttattct aacggcgccc aaattattga
     1681 tctcatctat tttgaccagg gtataaattt tattgttttt ttctaggatt tctgcaactt
     1741 ggtcgcttgt aacagcatct aagccacagc caaaggaatt aagctggacg agctctagat
     1801 ctttttgcct agcaacatag ctagcagcag aatacaatct agtatggtaa ctccactggt
     1861 ctattacccg aaggggcctt ggggcctttg ctaaatgaga aagagaagtc tcagaaagca
     1921 caacaaaacc ataggactga atcatttcag ggataccgtg gttaatttca ggatcaagat
     1981 gataggggcg tccagctagc aagatacctt ttttcttatt tcttttcata tagtccaagg
     2041 tttcttggcc ttttttttgt attttttcct tgtaggctaa taattctttg tagcctaaat
     2101 ctacggcttc ttttgtttcc ttaaaagaaa tgttttcttc ccttaattct tgaaagagtt
     2161 ttttagtgag aagcttttta tcatttaaat taacaaaggg cttatagaac tttattccta
     2221 aatcatcaaa ctcaaaattt gaatggatgg tttcaggata agaagttacc acagggcaat
     2281 taaagtggtt gcctgcctcc ttgtcttcta ttatggtata gggcacacaa ggatagaaga
     2341 tttttctaag ctttttattt attaggtcct gaatatggcc gtgggctatt ttagcaggat
     2401 aacaaacaga ttctgagggg atggtgttta tacccatttc atatattttt ttgttagagt
     2461 gtcctgacaa gactactcta taacccaatt ttgtaaaaaa agtaaaccag aaggggtaat
     2521 cctcatagat gttgagtact cttggtattc caataaggcc tcttggggcc ttatctagag
     2581 gcagggcctt atagttaaag acctctttat aattataggc atataggttt gggatctctt
     2641 ccttggtttt ttcttccttg gagctattgt ataattcagc ccctttttca catctattgc
     2701 tggacacaaa atatctacca tcattaaatt tacttatggt aagcaggcaa ttgttaccac
     2761 acaggccaca tcttttcatt tttgaagaaa tttcaaaggc ctccagttct tcttttttta
     2821 gtagacttga acttgctccc gtgtatcttt ctttggcgat aatagcagca ccgaaggctc
     2881 ccataatacc agctatatca ggcctgatta ctttttttcc tgttaagagc tcaaaagctc
     2941 ttaagacaga attgttgtag aaggtgcctc cttgaaccac gattttgtct cctaagtcat
     3001 caaaggaatt aaatcgtatg accttaaata aggcgttttt tataacggag atacttatac
     3061 ctgcagctat atcggccaca gtagctcctt ctctttgcgc ctgtttgacc tttgagttca
     3121 taaataccgt acatctagtc ccaagatcca caggatgctc agagaaatag ctgtttttag
     3181 cgaaagtttt tacatcggta tttaaagact tggcaaaggt ttcgataaaa gatccacagc
     3241 ctgaggaaca ggcttcattt aagcttacag aatcgataac accatttttt atttttaagc
     3301 tcttcatatc ttggccgcct atatcaagga caaaatcaag accactagaa aaatagtcag
     3361 cggctttata gtgggatatg gtttctattt ctcccatatc gacttgcaga gcggacttta
     3421 ccagatgttc tccatatccg gtaacagttg aattaacaat atggatctcc ttattatcca
     3481 aaatctcata aagatttttt agggcagaaa taacagactg gagtggattt cccctattgc
     3541 tatcataaaa agaatggaga agattcttat ccttatcaat aagtgcgatt tttgtagtgg
     3601 tggacccagc atctatgcca agaaaggctt cacccctata agagtttata tcctttcttt
     3661 tcacccttgc cttattgtgt cgatagatga attcatcgta ttctttctgc cccttaaaaa
     3721 gggggtccag ttcttttttt ctttgaaggg gctgattatt tctgtcctgc actttcttta
     3781 agaggtcccc ataattcatt gtttttgctt tttttgcatt aatggcggca cctagggcaa
     3841 caaaatattc tgagtttttt ggaaagataa cttccttatc gctcagagcc agagtgtcta
     3901 taaaacgctc tcttaattca gataaaaagg ttaaaggtcc tcctagaaag gcaatattgc
     3961 cccttatagg ccttccttgg gccagtccac tgatggtttg gtttactaca gcttgaaata
     4021 tggacgcagc aatgttttct ttgctggctc cttcatttaa taagggttga atatcagttt
     4081 tagcaaaaac tccacacctt gaagcaatgg ggtaaatggt attatggttt ttggctaatt
     4141 cattgagtcc ctgggcatct gtttctagca ggatggccat ttgatcgata aaggcccctg
     4201 ttccaccagc gcaggatcca ttcattcttt gctctaattg gccacggagg taggttattt
     4261 tggcgtcttc accaccaagc tctatggcta catctgtgtg ggggattttc tcttgaatgg
     4321 ctgtagtccc agcagccacc tcctggataa aaggtatatc aaaagcctta gatacatcta
     4381 gtgctcctga tcccgttgca tttatttttg ttttcttatc agctattata gtgtggcaat
     4441 cacttaacat agtaaaaagg gcttctctaa catttgaata atgtctttta tactctttat
     4501 gaactatatt gtcttgctca tccaagacta gaatttttac cgttgttgaa ccaatgtcta
     4561 atcctatatt aaatacacta ttcataagaa cctccaagga taataagcta aaacac
//
LOCUS       DASRNA010000159        12736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1095398,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000159 DASRNA010000000
VERSION     DASRNA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095398"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VFD08_04345"
     CDS             <1..1088
                     /locus_tag="VFD08_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZK10283.1"
                     /translation="SIGALNGALFALGKIDTLLDLYENFDVDLYFKFGEKDIERFDLD
                     DIKVTDLPQLTKMVYQLLKQGGVDITPLKSKLEEEVDENELRRSDINLGLVTLGIPKI
                     RPLEVFVEEIPQGKVHEYLIASAYLPFFKADERRYIDGWFVDNVPLDLLLKKGPFEQI
                     YVIRSHSGGLINSDWNREEVVLMTPSRPVGPSYNLNQKKMKENLLMGYYDMLRVLKGY
                     EGKRFYFKDLPDLKKVLLNEEVLRQFKKRFSKLEDYDVQRYFYEELLPGLAKSLNLGV
                     KSTYQEIFLELLEAGGYYLDIYPNRVYDIETYIEELREALSKKEVEWNLKEKVFSEVA
                     KYTWGWFGDLQSLVLAFLDLMVSCQGR"
     gene            1091..2695
                     /locus_tag="VFD08_04350"
     CDS             1091..2695
                     /locus_tag="VFD08_04350"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HZK10284.1"
                     /translation="MDRFEQELMDPQYDNAYHNAMLALSREEKYDSYYKKLEFGTAGM
                     RGKRGVGPNRMHIYQVRRVSAAFAQYLLEESESPSCAIAFDSRHGSLEFSRETAVTMA
                     SRGVKVHLFRDFSATPELSYAMRELRATGGVVITASHNPKEYNGYKVYHSSGRQLLED
                     EAEKIIEHLERLPSTLNNQSYSELIEEGLIEFISIDLLENYDKTIFSGIKRSSNQELK
                     VVYSALHGVAGRGTQSLLKRMGVECIPVQSQIEPDGDFPEVHYPNPEDVSVFEKAIEI
                     GEKEEADLLLASDPDGDRLGVMVAHRGSYQPLTGNEIGLLMAQYLLSIEAGEIITSIV
                     STYLLDELAKKYNVRLTRTLTGFKYIGERMNHIYNFVLGFEESYGYLSLDHARDKDAI
                     QAAGRLCEMARVYKEEGRTLVDVLEELYLTYGYEKNTQIVYELEGAKGAEKIQKMMED
                     FRNNPLSELAGAGLLKSIDYLKEETGLPKANVLMRFYEGGHWLAYRPSGTEPKLKIYV
                     GIRSKTREEAEEIYERAYKELEESSL"
     gene            2950..3609
                     /locus_tag="VFD08_04355"
     CDS             2950..3609
                     /locus_tag="VFD08_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZK10285.1"
                     /translation="MKKERIKKPISYKAQVYEAILEEITTHKINSDQIYSEQWLADYF
                     SVSRTPVREALLQLRAEGFVQTLPNRGFVVKPMLLEDAYTIFQTRAAIEGYCAAHLAR
                     NIDSPEALSTVQEINLLLKKVSKNFNREDEIDFHLEIIKFSKNPEFVNIFKNMRAKID
                     IYWTEVVNIENRPEEAQREHEVIMESILAGDDYGAFRASEDHLFTTYNMIKDSDLFKK
                     K"
     gene            3784..4773
                     /locus_tag="VFD08_04360"
     CDS             3784..4773
                     /locus_tag="VFD08_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HZK10286.1"
                     /translation="MSNFKVLMTDSIFPDQEKERAMLEEIGAELLLSDSQDPAQLAKL
                     AKDCDAIIVTFAEVTREVISACEKCKVIVRTGIGVNNVDVKAATEKGIIVANVLNYCI
                     PEVADHAMALMLASLRKIIVYNKVVRCGVWDMNQGSISRIGSLTLGLYGFGNISREVA
                     LRAKAFGMKVVAYDPFLEDSIFEEYGIERKVDLEEFIQSADVLSLHLGLNEHTYQIIN
                     KKTFELMKESAILVNVSRGGLVNEDDLLYAIENNLIAGCALDVMADEPGDMHSRLLTY
                     KNVIVTPHAAFYSEESNEELRTKATEQVLQTLTKGYPEFWVNKKELETKGERK"
     gene            4770..5993
                     /locus_tag="VFD08_04365"
     CDS             4770..5993
                     /locus_tag="VFD08_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HZK10287.1"
                     /translation="MKKPWHQEKWFTSSWNFADEVTRDFNFAKDIKFHDVSLRDGEQQ
                     AGLIFTKDQKIALAEKMAELGMHRIEAGMPAVSKQDRDAIIEIEKRNLGMDVFAFARC
                     MREDVLRAVDCGVKNIVIEIPASEHVVEHAYKWEYEKAIELAIDATQCAKENGLYTVF
                     FPIDGTRTELGTFTELITRVANEGHMDALAVVDTFGGLAPAACSYLIKKIKTVIDKPL
                     EVHFHDDFGLGAANTLMALSAGAEVAHTTITGIGERAGNAAYEDVALSLLTMYGIDTG
                     LKYEKMNELSKMMRDFSGHTIAPNRGIVGPTLADIESGLVADWYANAHKEHPLELSPY
                     DYSLVGHESAKVVIGKMSGISTVDIYLDQLGIECDSREAKLEIVAKIKEKAFLKHGLL
                     TVEEFREIVFKVLGK"
     gene            6042..7142
                     /locus_tag="VFD08_04370"
     CDS             6042..7142
                     /locus_tag="VFD08_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZK10288.1"
                     /translation="MFKRSVSIMVLLLLIFALVSCGAPAEEKAEETSQEAVAEEEEAV
                     EEEEEAVEVDGEVFQIKFANNGVSDMAHPQNEAMTFFKDTVEELSEGRIKVSLHIAGS
                     LGDARTIMEGVQLGTIEMGDVENGVMSGFVPEAGIFDLPYIFDDLEHAHAVQAGEVGD
                     YLREEYLDIGVRNLAFNDGGFRYFTTSKRALTDAESFDGMKIRVMESDIMIKTINAFG
                     GSAVPMAFGELYTALQQGTVEGQENPLDLIYAQSYAEVQDYLSLSEHFYYPRQYIINE
                     DFYQSLPAGLQEIIADTALEACQKQREFLVEYTKSMMGELKEQGFEVVEFDKEYFQKL
                     STDLWPEFYASVGNGDEELGKWLVDSVRAEVQ"
     gene            7214..7690
                     /locus_tag="VFD08_04375"
     CDS             7214..7690
                     /locus_tag="VFD08_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HZK10289.1"
                     /translation="MIQKILDKAIKYLLVIAMMLISALSFYQVVTRYAFNNPSTWSEE
                     LIRFIFVWASCLGAAIGIKEHIHIGIDVIVNFFSPKIRMVVDVIIHLVLGVFAVFMIK
                     YGYALTLKTRVQLSPALRIPMSYVYLAVPVMGVLIIFYASLEIARILKQKGLEEQC"
     gene            7684..8964
                     /locus_tag="VFD08_04380"
     CDS             7684..8964
                     /locus_tag="VFD08_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZK10290.1"
                     /translation="MLTVLVVVLLVCFVFNIPIGFALGVASIGSLLAGGNPDQLIMVP
                     QRIVASMNSFPLLAIPLFMVAGSVMEIGGVSKRIVTAASSLIGHIRGGLASVSIMACT
                     FFAAISGSTPATAAAIGSLTIPEMRQRGYPDDYSSAVVAASSCLGVVIPPSITMVVFG
                     VTGNISIGKMLIGGVIPGLLIASMLIATNVYFSRKADFPTEPKESRKQRLTALKEAFW
                     ALMMPVIILAGIMSGVFTPTESAAVAVVYGLIVSLFIYKEMSLKDLVPVFYKAALNSA
                     MIMLLIGTAGPFGWIMTVQDVPNMVSTAILSISDNYYVIFSLVLLIYLILGTFMETAA
                     IIMLTVPILTPIMVNIGADMVHYGVVSVIALAIGMATPPVGIALFATCGVSKVTMAQI
                     SKQIWPFLLVMILALFVLMLVPQIVTFLPNLMMG"
     gene            8990..10153
                     /locus_tag="VFD08_04385"
     CDS             8990..10153
                     /locus_tag="VFD08_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HZK10291.1"
                     /translation="MVKKKLALKKHREWKGKLEIVTRSPVENKEDLAIAYTPGVAEPC
                     LEIQADESLAYDYTGKGNLVAVISDGTAVLGLGDIGPSASMPVLEGKCALFKKFADIN
                     AIPICIDSKEVDEIVSAITLISKSFGGINLEDISAPRCFEIEKRLVETLDIPVFHDDQ
                     HGTAIVTAAALINAVKLVKKDPSTIRIVISGAGAAGISIARLLINMGFGDILLCSSKG
                     AIYEGAPDNNSEKEEIAKITNRSQRRGSLEDVIKGADVLIGVSMPKIVSEEMVASMAD
                     KPIVFGLANPIPEIMPEAAKRAGAAIVATGRSDYPNQVNNVLAFPGIFKGALLARAPG
                     ITEEMKVVAARAIAQVVSEEELSPDYILPDAFNEEVVKSVSRAIIKEAERNKS"
     gene            10450..11697
                     /gene="larA"
                     /locus_tag="VFD08_04390"
     CDS             10450..11697
                     /gene="larA"
                     /locus_tag="VFD08_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HZK10292.1"
                     /translation="MKVKLNIGKTHQYLEVSDKAAVSILQKKELTFSKKGVDAVEDSL
                     DYPLGTLKLEEIVKPGEKIVIVTSDVTRPMPSDKVLPSILERLYKAGCKKEDISIVFA
                     LGSHRRQSKEEMIQLVGKEVYESVKCLDGDMEDVVHMGRTSAGTPVDIVRVVAEADRR
                     ICLGNIEYHYFAGYSGGAKAIMPGVSTRKAIQSNHSKMVHRRAVAGNLDDNPVRIDLE
                     EAIHYCPIDFICNVILDENKEIIYSVAGDYIKAHRHGADILDTIYCAEIDQKADIVVV
                     SQGGSPKDSNLYQTQKALDNAKHAIKKDGIIILLGSCEEGMGDEVFEDWMLKAKSPQD
                     LINRIEKKFKLGGHKAAAIGLVLEEAEIYLVSEMEKDFVESIFMTPFASVQAAYDRAR
                     KKFEKEATVIVMPYGGSVLPRLK"
     gene            11756..>12736
                     /locus_tag="VFD08_04395"
     CDS             11756..>12736
                     /locus_tag="VFD08_04395"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HZK10293.1"
                     /translation="MIQNKLTIIEAKGTPYEIGLKHGSLGKKEVLKSIENYTKMFKTY
                     AKIDWEEAKTTSRQYIEKIEEFDKDILEEMRGIADGAGVELEDILALNARSEVILMSN
                     RGAQLDGCTSLVALPEYTSEGNTLLAQNWDWKEEQRQALIYLTIESPNHPKIMMITEG
                     GIVGKIGFNSSGLGVCLNALGMDNFNPEGVPLHVVLRGILNSSTLSDAIKRVNEIPNA
                     CAANYLMGHGGGEAVSLEKAPHDFDILYPSEGTLVHSNHFITPRLRSKDTSRFMFHDT
                     FVRYGRADRLIRKEVGKIDVESFKKILSDHVEYPDSICRHSDPKDDPEERM"
BASE COUNT         4146 a         2266 c         2862 g         3462 t
ORIGIN      
        1 ccagtatagg ggcccttaat ggcgctcttt ttgccctggg caaaattgac accctacttg
       61 atttatatga aaactttgat gtggatttat actttaaatt tggtgaaaaa gacatagaaa
      121 ggtttgatct tgatgatatt aaggtaactg atcttcccca gctaacaaag atggtctacc
      181 agcttttaaa acaaggggga gtggatatta cccctcttaa aagtaaacta gaagaagagg
      241 tagatgaaaa tgagctccgc cggagcgaca taaatttagg tcttgtcacc ctaggcattc
      301 caaagataag gccactggaa gtatttgtag aagaaattcc ccagggcaag gtacatgagt
      361 atcttatagc cagtgcctac ttgccttttt ttaaggccga tgagcgccgc tacatagatg
      421 gctggtttgt agataatgtt ccactggatt tgctcttaaa aaaaggcccc tttgaacaga
      481 tttatgttat tcgttctcat agtgggggac ttataaatag cgactggaat agggaggaag
      541 tagttctaat gactcccagc cggccagtag gccccagtta taatctaaac cagaaaaaaa
      601 tgaaagaaaa tctccttatg ggatattatg atatgcttcg ggtcctaaag ggctatgagg
      661 gcaaaagatt ttattttaaa gacttgccag atttaaagaa ggttttactc aatgaagagg
      721 tcttaagaca attcaaaaaa cgattttcca agttagagga ctatgatgtg cagaggtatt
      781 tttatgaaga acttttgcct ggacttgcta aatcccttaa tcttggggta aaatcgactt
      841 atcaggagat ctttttggag ttgctagagg cagggggata ttatttggat atttatccca
      901 accgtgtcta tgatatagag acctacatag aagagttaag agaagcacta agtaaaaaag
      961 aagtagaatg gaatcttaaa gagaaggttt ttagtgaggt agccaagtat acctggggct
     1021 ggtttggaga tcttcagagc ctggtccttg cctttctcga tttgatggta agttgccaag
     1081 ggaggtaaaa atggatagat ttgaacaaga actaatggat ccccagtatg acaatgccta
     1141 tcataatgcc atgctggctt taagccggga agaaaaatat gacagctact ataaaaagct
     1201 tgaattcgga acggcaggca tgagggggaa aagaggagtg ggacccaata ggatgcatat
     1261 ctatcaggtc cgcagggtca gtgcagcctt tgcccagtat cttttagaag aaagtgaaag
     1321 tcccagctgc gcgattgcct ttgattctcg tcacggttcc ctagagttct cccgggagac
     1381 agcggttacc atggccagtc gcggcgttaa ggtccattta tttcgagact tttctgccac
     1441 accagagcta agttatgcta tgcgagagct tagggccaca ggaggcgtgg tgattaccgc
     1501 cagccataat cccaaggagt ataatggcta taaggtatat cattcttcag gaaggcagct
     1561 gctagaagat gaagcggaaa agattattga gcatctagaa agactcccca gcaccctaaa
     1621 caatcaaagc tatagcgagc tgattgagga gggcctaatt gagtttatct ccatagacct
     1681 cttagaaaat tatgataaga ccattttttc ggggataaaa agatccagta atcaagagtt
     1741 aaaagtggtt tattctgccc ttcatggtgt agcaggaagg ggaacccaga gtctacttaa
     1801 gcgtatgggt gtggagtgta ttcccgttca aagccagatt gagcccgatg gagatttccc
     1861 cgaagttcat tatcccaacc ccgaggatgt cagtgtcttt gaaaaggcaa tagaaatcgg
     1921 agaaaaagaa gaagcagacc tgcttcttgc tagcgatcct gatggagacc gcctgggagt
     1981 tatggtggct cataggggaa gctatcagcc cttaacaggc aatgagattg ggcttttaat
     2041 ggcccagtat cttctaagta tagaggcagg ggagattatc acctccattg ttagtaccta
     2101 cctgcttgat gagctggcaa aaaaatataa tgtccgccta actagaaccc taactggttt
     2161 taagtatatt ggcgagcgca tgaaccatat ctacaatttt gttctaggct ttgaagagag
     2221 ctatggctat ctttccctag accatgccag agacaaggat gccatccaag cagcaggcag
     2281 actatgtgag atggccaggg tctataaaga agaaggaaga accctagttg atgttctaga
     2341 agagctctac ttaacctatg gctatgagaa aaacacccag attgtctatg agctagaagg
     2401 ggccaagggg gcagaaaaaa tccagaagat gatggaggat tttagaaata accccctatc
     2461 agaacttgca ggagctgggc ttcttaagag tattgactac ttaaaagaag aaacaggact
     2521 tccaaaggca aatgtcctta tgcgctttta tgaaggaggc cactggttgg cctatcggcc
     2581 ctcaggaaca gaacccaaac taaaaattta tgtgggcatt cgaagtaaaa caagagaaga
     2641 agccgaagaa atctatgaaa gggcatataa ggagctagaa gaaagttcct tataaagcct
     2701 atctgcagtt ttatacaagc tagctagaac ccccccggtg ctcggcaaag aagctagcac
     2761 cgggggagtt tcttttgccg tagtaatttt tccggatgac aagcaggccg ctttatggct
     2821 atttagtatg taaataagga aataaaaaaa gataatgata gcaaagtatt gacaagcgca
     2881 atgactaaat ggtagtatac tagtgaaacg tagcatacta ctcaaatcag aagccaaagg
     2941 gtaaagggaa tgaaaaaaga aagaattaaa aaacccatca gttataaagc ccaagtctat
     3001 gaggctatct tagaagagat aaccacccat aaaataaata gtgatcaaat atattcagag
     3061 cagtggcttg ctgattattt ttctgtttct agaacccctg taagagaggc tcttcttcaa
     3121 ctaagggcag aaggcttcgt tcaaacctta ccaaatcgtg gctttgttgt aaaaccaatg
     3181 ttgcttgaag atgcctatac aatatttcaa acccgtgctg ctattgaagg atactgcgcg
     3241 gcccatctag caaggaacat tgatagtcca gaagccttgt ctacagtcca agagataaat
     3301 ttacttctta aaaaagttag taaaaacttt aaccgagaag atgaaataga tttccacttg
     3361 gaaatcatca aattttccaa gaaccctgag tttgtaaata tatttaaaaa catgagggcc
     3421 aaaatcgata tatactggac agaggtagtc aatatagaaa acagaccaga agaagcccaa
     3481 agagaacacg aagtaattat ggagtcgatt ctagcgggtg atgattatgg tgcctttaga
     3541 gccagtgaag atcatctttt tactacctac aatatgatta aagacagtga tctctttaaa
     3601 aagaaataaa aggtgtgaca ttaaaaaaac acttttttat ttataggaac tagtatgtta
     3661 catactttaa taaaaaacaa cagaattttc cctgttctta cccagttttt tatttgcttg
     3721 tactagtatg taacatacgt cccatcttta actctacaca aaacacttag gaggtgtact
     3781 ggaatgtcaa acttcaaagt attgatgaca gattcaatat ttccagacca agaaaaagaa
     3841 agggctatgc tagaagaaat cggagcagaa cttcttctgt ctgattctca agacccagcc
     3901 caactagcaa agctcgcaaa agactgcgac gcgattatag taacctttgc agaggtgact
     3961 agggaagtaa tttctgcctg cgaaaagtgc aaggttattg taagaacagg tattggagtc
     4021 aacaatgttg atgtaaaggc tgctacagaa aaaggaatca ttgtagccaa tgtgcttaac
     4081 tactgtatac cagaagttgc ggaccatgct atggccttaa tgctagccag ccttagaaaa
     4141 atcatagtct ataacaaggt agttcgctgc ggtgtatggg atatgaatca aggtagcatc
     4201 tcaaggattg gaagcctaac ccttggactt tatggatttg gaaatatttc tagagaggtt
     4261 gccctaaggg caaaggcctt tggaatgaag gtagtagcct atgatccttt cttagaagat
     4321 tcgatatttg aagaatatgg tattgaaaga aaagtcgatt tagaagaatt tatccagtcc
     4381 gctgatgttc tttctttaca tctaggtctt aatgagcaca cctatcaaat cataaataaa
     4441 aagacctttg agctaatgaa agaaagcgct attttagtta atgtttctag gggcggacta
     4501 gtaaatgaag atgatttact ttatgcaatt gaaaacaatc taattgcagg atgcgcccta
     4561 gatgtaatgg cagatgagcc gggtgatatg cattcaagac tattaaccta taagaatgta
     4621 attgtcaccc cccatgcggc cttttactca gaagaatcaa atgaagaact aagaaccaaa
     4681 gcaacagaac aagttctaca aacacttacc aaaggttatc cagaattttg ggtaaacaaa
     4741 aaagaattag aaacaaaagg agaaagaaaa tgaaaaaacc ttggcatcaa gaaaaatggt
     4801 ttactagctc ttggaacttt gctgatgagg taactagaga ctttaacttt gctaaagaca
     4861 tcaagttcca cgacgtttcc cttcgtgatg gcgagcaaca agcaggtctt atttttacaa
     4921 aggatcaaaa gattgcccta gcagaaaaaa tggcagaact aggcatgcat cgtattgaag
     4981 ctggtatgcc agcagtatcc aagcaagata gagacgccat tatcgaaatt gaaaaaagaa
     5041 atctaggaat ggacgttttt gcctttgcta gatgtatgcg tgaagatgtt ctaagagccg
     5101 tagactgcgg agtaaaaaac attgttattg aaattcctgc tagtgaacat gttgttgaac
     5161 atgcctacaa atgggagtat gaaaaagcaa tagaacttgc tatagatgca acacaatgtg
     5221 ccaaagagaa tggattatac acagtgtttt tccccatcga tggaacccgt acggaactag
     5281 gaacttttac cgaactaatt actagagtag ccaatgaagg tcatatggac gccctagcag
     5341 tggtagatac ctttggtggt ttagcaccag ctgcatgttc ctatttgata aagaaaataa
     5401 aaacagttat tgataagcca ctggaagtac acttccatga tgactttggt ctgggtgcag
     5461 caaatacact tatggcccta tctgcaggag ctgaagtagc tcacacaaca ataacaggta
     5521 taggcgaaag agcgggtaat gctgcctatg aagatgttgc cttgtcttta ctcaccatgt
     5581 acgggattga cacaggtcta aagtatgaga aaatgaatga gctctcaaaa atgatgagag
     5641 atttttcagg acatacgatt gctccaaata gaggaattgt tgggcctacc ctagctgata
     5701 ttgaatcagg acttgttgca gactggtatg ccaatgcaca caaggaacat ccactagagc
     5761 tttcccctta tgattattca ctggttggac atgagagtgc caaggttgta atcggaaaaa
     5821 tgagcggaat ttctacggta gatatctact tggatcaact aggaattgaa tgtgatagta
     5881 gagaagctaa acttgaaata gttgctaaaa tcaaagaaaa agctttctta aagcatggtc
     5941 tgctaactgt ggaagagttt agagaaatag ttttcaaggt tcttggaaaa taaaacataa
     6001 ctgttaaaac caaacgaaag catagcataa tggaggctaa aatgtttaaa agatcagtga
     6061 gtataatggt tcttctttta ctaatttttg cactagttag ttgtggagct ccggcagaag
     6121 aaaaagcaga agaaacaagc caggaagccg tcgcagagga agaagaagcg gtagaagaag
     6181 aggaagaagc tgttgaagtt gatggagaag ttttccagat aaaatttgca aacaatggcg
     6241 tttctgatat ggctcatcca caaaatgaag cgatgacctt ttttaaagac acggtggaag
     6301 aactctcaga aggccgcatt aaagtcagcc tgcatatcgc cggatctctt ggtgatgcgc
     6361 gcacaatcat ggaaggcgtt caacttggaa ccattgaaat gggagacgtt gaaaatggcg
     6421 taatgtcagg ttttgtacct gaagcaggaa tctttgattt accctatatc tttgatgatt
     6481 tagagcatgc tcatgcagtt caggccggag aagtaggaga ttatttacgc gaagaatacc
     6541 tagatatcgg cgtaagaaac ctagccttta acgacggtgg ttttagatat ttcacaacaa
     6601 gtaaaagagc ccttaccgat gcagaatcct ttgatggcat gaaaataagg gttatggaaa
     6661 gcgatattat gatcaaaaca ataaacgctt ttggtggctc agctgttcct atggcctttg
     6721 gtgaattata cactgcacta cagcaaggca ccgtagaagg acaagaaaat cctttagacc
     6781 taatctatgc ccaaagttat gcagaagtac aagactatct ttcactaagt gaacacttct
     6841 attatccaag acaatacatc attaatgaag atttttatca aagcctacca gcaggtctac
     6901 aagaaatcat tgctgataca gcccttgaag cctgtcaaaa gcaaagagag tttctagtag
     6961 aatacactaa atctatgatg ggtgaattaa aagagcaagg ttttgaagtt gttgagtttg
     7021 ataaagaata ttttcaaaaa ctatcaacag acctgtggcc agaattctat gcttcagtag
     7081 gaaacggaga cgaagaacta ggaaaatggt tagtcgattc tgttagagct gaggttcaat
     7141 aagagacttt atcaacgata tagggcagcc taaatttata atctaggctg cccttttgaa
     7201 agaagggaat tatatgattc agaaaatcct tgataaggca attaaatatc tactagtaat
     7261 tgctatgatg ttgatttctg ctttatcatt ctaccaggta gtgacccgtt atgcctttaa
     7321 taatccttca acttggagtg aagaattaat cagatttatc tttgtttggg cctcctgtct
     7381 gggtgcagct atcgggataa aagagcatat acacattgga atcgatgtta tagttaattt
     7441 cttttcgcca aaaattagga tggttgttga tgtaatcatc catttggttt taggtgtttt
     7501 cgctgttttt atgattaagt atgggtatgc actcacccta aaaactagag tgcaattaag
     7561 tcctgctctt aggataccta tgagctatgt ttacctagca gttccagtaa tgggtgtttt
     7621 aataatcttt tacgcctcat tagagattgc aagaattcta aagcagaaag gattggaaga
     7681 acaatgttaa cggtattagt agttgtatta ttggtctgct ttgtatttaa cattcctatt
     7741 ggctttgctt taggtgttgc atccatagga agtttactag caggaggaaa cccagatcag
     7801 ctgattatgg tccctcaaag aatagtagca agtatgaact cttttcctct acttgccatc
     7861 cctttattta tggtggcggg ttcagttatg gaaataggtg gtgtttctaa gagaattgtt
     7921 acggcggcct cttccctaat tggtcatatc cggggaggac tggccagtgt ttctattatg
     7981 gcctgtactt tctttgctgc tatttcaggt tcaacaccag ctacagcagc agcgattgga
     8041 tccctgacca tacctgaaat gagacaaaga ggctatcctg atgactattc atcagcagta
     8101 gtagccgcct catcttgttt gggagtagtc attcctccaa gtataaccat ggttgtattt
     8161 ggtgttacag gaaatatatc cattggaaaa atgctaattg gtggagttat tcctgggctt
     8221 ttgattgcca gcatgctgat tgccacaaat gtatatttta gtagaaaggc tgattttcct
     8281 accgagccaa aggaatcaag aaagcaaaga cttacggcac ttaaggaagc cttttgggca
     8341 ctgatgatgc cagtaattat actagctgga attatgagtg gagtctttac tccaacagaa
     8401 tcagctgctg tggcagtggt ttatggtctc atagtcagct tatttatcta caaagaaatg
     8461 tctcttaaag acctagtccc agtattttat aaggcggcct taaactcagc tatgattatg
     8521 ttattaattg gaacagctgg accctttggt tggataatga cggtacaaga tgtgccaaat
     8581 atggtttcca cggccatctt gtccatttcg gataactact atgtaatctt tagcctagtc
     8641 ttactaatct acttaatttt aggaaccttt atggagacag cggctattat catgctaacg
     8701 gtgccaatac ttacccctat tatggtaaat attggagcgg atatggtcca ttatggcgta
     8761 gttagtgtaa ttgctttagc cattggaatg gccaccccac cggttgggat tgccttattt
     8821 gcaacctgcg gtgtatcaaa agtaaccatg gctcagataa gtaaacaaat ctggcccttc
     8881 ttactggtga tgatcttggc cctatttgtc ttaatgctag ttcctcaaat agttaccttc
     8941 ttaccaaatc ttatgatggg ttaataatag acaaagctta ggagaaaaga tagtgaaaaa
     9001 aaaattagcc ttaaaaaaac atagagagtg gaaaggcaag cttgaaattg taacaagaag
     9061 tcccgttgaa aacaaggaag atttagccat tgcctataca cctggagtgg ctgagccctg
     9121 ccttgaaatt caagctgatg aatccttggc ttatgactac acaggcaagg ggaatctagt
     9181 tgctgttata tccgatggga ctgctgtatt aggcctaggg gatattggac catcagccag
     9241 tatgcctgtt ttagaaggaa agtgtgcttt atttaaaaag tttgcagata tcaatgccat
     9301 tcccatctgt atcgattcca aagaggtcga tgagattgtt tcggccataa ccttgatttc
     9361 caagagtttt ggcggaataa atttagaaga tataagcgcc cccagatgtt ttgaaatcga
     9421 aaaaagattg gttgaaacac tggatatacc agtttttcac gatgatcaac atggaacggc
     9481 cattgttact gcagcagccc taatcaatgc agttaaatta gtaaaaaaag atcctagtac
     9541 aattaggatt gttatttccg gagcaggggc agcaggaata tcaatagcca ggctcttaat
     9601 aaatatgggt tttggagata tccttctttg ttcaagcaag ggggccatct acgagggggc
     9661 tccagacaac aatagtgaaa aagaagaaat tgccaagatt accaatagat ctcagcgtag
     9721 gggctcctta gaagacgtga tcaaaggcgc tgacgtttta attggtgtgt caatgcccaa
     9781 gatagtaagc gaagaaatgg tcgcttctat ggcagataag cctattgtat ttggactggc
     9841 caatcctatt ccagaaatta tgcctgaagc cgccaaaagg gctggggcag ccattgtggc
     9901 tacaggccgc tcagattatc ccaatcaggt caacaatgtc ctagcctttc cagggatttt
     9961 taaaggcgcc ttactggcca gagctccagg cataacagaa gagatgaagg ttgttgccgc
    10021 aagagcaatt gcacaggtag taagcgagga ggaactaagt cctgattata ttttacccga
    10081 tgcctttaat gaagaagtag ttaaaagcgt ctctagggca attataaaag aggctgagcg
    10141 aaacaagtcc tagcctagag acttcatcct tttgataaaa atcgtatcta aattaaagtt
    10201 gagggtaagc ttgtaaagca agttttacat tctaatttcc gccttggtat agcagcttga
    10261 tataaagctt gaggcctgct ttcaactttt tgatggatac gattttttga actttacttc
    10321 ctgataatat atactttaga ggacttctac acttggtttt caaagattag aggtccttag
    10381 attgggaaaa aacactttta aatagaggca gaaaaaagaa aaaaagttct ttaagatttg
    10441 aggttgccca tgaaggttaa attaaatata ggaaaaacac atcaatacct agaagtcagt
    10501 gataaagcgg ctgtaagcat tttacaaaaa aaagagctta ctttttccaa aaaaggagtt
    10561 gatgcagtag aagactccct agattatccc cttgggacgc ttaaactaga ggaaatagta
    10621 aagccaggtg aaaaaatagt tattgtcacc tcggatgtaa ctagacctat gccctcagat
    10681 aaggttcttc cttccatttt agagcgccta tataaggcgg gctgtaagaa ggaagatata
    10741 agcatagttt ttgccctggg ttcccataga aggcaaagca aggaagaaat gatccagctg
    10801 gttggtaaag aggtatatga atccgtaaaa tgccttgatg gggatatgga agatgtagtt
    10861 catatgggta gaaccagtgc aggaactcct gttgatattg taagagtagt agcagaagcc
    10921 gaccgaagaa tttgcctggg aaatattgag tatcattatt ttgccggcta cagcggggga
    10981 gcaaaggcaa ttatgcctgg tgtttcaact agaaaggcca ttcaatccaa ccacagcaaa
    11041 atggtccata ggagggcagt tgcaggtaat ctggatgata atccagtgcg tattgattta
    11101 gaagaagcaa ttcactactg ccccattgat tttatttgca atgtaatttt agatgaaaac
    11161 aaagaaatta tctattctgt agccggggac tatataaagg cccaccgcca tggagcggat
    11221 attctagaca ctatttactg cgcggaaatt gatcaaaagg ctgatatagt agttgtttca
    11281 caagggggaa gccccaagga ttctaatctt tatcaaacac aaaaggcact agacaatgcc
    11341 aaacatgcca tcaagaaaga cggtattatt atactgctag gttcttgtga agagggtatg
    11401 ggagatgaag tttttgaaga ctggatgctc aaggccaaaa gcccccagga tcttatcaat
    11461 cgaattgaaa agaaatttaa gctgggaggg cataaggcag cagccattgg ccttgtactt
    11521 gaagaagccg aaatttatct tgtatcagaa atggaaaaag attttgtaga aagcatattt
    11581 atgaccccct ttgccagcgt gcaggcagca tatgataggg caagaaaaaa atttgaaaag
    11641 gaagcaactg tgattgttat gccctatggt ggttcggttc tacctcgctt aaaatgataa
    11701 ataatcctac agcagtttta taaattacat ttgtataaga aataaggagg gtgaaatgat
    11761 tcaaaacaag ctaactatta ttgaagctaa gggaacacct tatgaaattg ggctaaaaca
    11821 tggtagccta ggaaaaaaag aagttcttaa aagtattgaa aattacacaa aaatgtttaa
    11881 aacctatgcc aagatagact gggaagaggc caaaacaaca tccagacaat atattgaaaa
    11941 aatcgaggaa ttcgataaag atatactaga agagatgagg ggaatagcag acggggctgg
    12001 agtggaacta gaagacattt tagcgctaaa tgctcgaagc gaagtaatac taatgagtaa
    12061 taggggggcc caactagatg ggtgcacctc acttgtggcc ctacccgagt atacttctga
    12121 gggaaataca ctcctggccc aaaactggga ctggaaagaa gaacaaagac aggcacttat
    12181 ttatctaaca atcgagtccc ctaaccatcc aaaaattatg atgattactg aaggcgggat
    12241 tgttggaaag ataggtttta attcttctgg actaggagtg tgcttaaacg cccttggtat
    12301 ggataacttt aatccagaag gtgtcccctt gcatgtagtt ttaaggggaa tcctaaatag
    12361 ctcaacccta agtgatgcca tcaaacgcgt aaatgaaatc cccaatgctt gtgcagccaa
    12421 ctatttaatg ggtcatggcg gcggggaagc agtgagctta gaaaaagccc cccacgactt
    12481 tgatatactc tatcctagcg agggcactct tgttcactcc aaccatttta ttacgccaag
    12541 gttacgctca aaagacacct cccgatttat gtttcatgac acctttgtta gatatggcag
    12601 ggcagatcgg ctaataagaa aagaagtcgg caaaatagat gtagaaagct ttaagaagat
    12661 cctaagtgat catgtggagt atccagattc catttgtaga cacagtgatc ccaaggatga
    12721 tcctgaggag agaatg
//
LOCUS       DASRNA010000160         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1095884,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000160 DASRNA010000000
VERSION     DASRNA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095884"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_binding    184..444
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            515..1714
                     /gene="ilvA"
                     /locus_tag="VFD08_04400"
     CDS             515..1714
                     /gene="ilvA"
                     /locus_tag="VFD08_04400"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005917482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HZK10294.1"
                     /translation="MLRLEDFVEARARLQGVISKTELIHSPIFSEETGNEIFIKPENL
                     QLTGSFKIRGAYNKMAKLSQEEKEKGVIASSAGNHAQGVALAGKMLGIQTLIVMPKYT
                     PLIKVEASRSYGAKLVLYGEDYDQAYKKALSLKEKGYNFVHPFDDQDVIEGQGTIALE
                     VLEELADADIIMVPLGGGGLISGIAAAAKLKNPQIKIIGVEAEGAASASYSLARKRLE
                     ELPRVHTIADGAAVKRIGEIPFAYIQKYVDEIVTVSDYELMEEFLILVEKHKIIAENA
                     AILSIAGLKKIKEKNKKIVSILSGGNIDVLTISSMINKGLVSRERILKFSLGLPDKPG
                     QLVNISQILAAQNANVIKLDHNQCKNLNRFHHVELQVTVETNGEEHISSIIEALYKAG
                     YQIKRLN"
     gene            1738..3444
                     /gene="ilvB"
                     /locus_tag="VFD08_04405"
     CDS             1738..3444
                     /gene="ilvB"
                     /locus_tag="VFD08_04405"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012859590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZK10295.1"
                     /translation="MEKKWIKGSEILLESLSRLGVDTIFGYPGGSVIPIYDALYDFKK
                     ISHYFARHEQGAAHEADGYARATGKVGVCFATSGPGATNLVTGIMTAHMDSVPLMAIT
                     GQVGSNLLGKDAFQESDIVGITIPITKMNYLVKDIKELPRIIKEAHFVAHTGRPGPVL
                     IDLPKDIQLAEIELAEFEKLFTEDIQLEGYVPNYKGHPNQIKKAIDLIKKAEKPLIIA
                     GAGIIKSKACAELKELAQTMDIPVTTTLLGLGSFPSSHRLSLGMLGMHGTVGANFATQ
                     EADLVIALGMRFDDRIAGDPENFCHLAQIIHIDIDPAEIEKNRMVEIPIVGDLKGVLK
                     AINKKLTPRKNTDWVEKILGWKKEYPMVYGKSSEKLMPQEVLEHLDTIVQGQAIVSTD
                     VGQHQMWVAQFLQFTKPNTMLSSGGAGTMGFGLPAAIGAQVGRPEELVIAVVGDGGFQ
                     MTYQELMLIKQYQLPVKVLLLNNSYLGMVRQWQDLFLDKRYSSVSLDINPDFEKIGEA
                     YGIKTVTLKTRGELEDQLEGLIKSKEPVIIQCIVEGEANVLPMIPAGKCANHMIGLRG
                     VI"
     gene            3444..>3786
                     /gene="ilvN"
                     /locus_tag="VFD08_04410"
     CDS             3444..>3786
                     /gene="ilvN"
                     /locus_tag="VFD08_04410"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012859589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZK10296.1"
                     /translation="MDQYEILIIAKNKDGIIGRILSLFNKRGYFVRKMTAGATNRAGY
                     ARLTLTVEGDELTLEQIQKQVYKIIDVVKVKIFPSKGVIRREMMLIKVKASNETRTQI
                     IQIADIYRGSVL"
BASE COUNT         1318 a          714 c          874 g          880 t
ORIGIN      
        1 atactaggga ttttttcatt tcttggccct ttttatccct taaagataat cttgatagct
       61 atgacttctt aggaaaaaag aagaaaaggc aggctaaaca aagacaagca agaagaggaa
      121 aaaagataca taagtgttag aaaaagtcag gatataacct attagaccta aaaaataaat
      181 aagataaaag taatgacgga gacaaaagta tacaaggatt aattacagag aactgacaaa
      241 agctgagagt cagcattaac aagtatataa tgatccctcc agaactgtcg agatgaaata
      301 aaacaaagta gtcaagaccg ggattcttag aagataagaa gcgccaccgt tatatggcta
      361 gggctagact ttttggccta aagagtcagt tttcgtgaga aaactgagaa gcttgggtgg
      421 taacacgata cattcgtccc tagtatagaa atatactagg gattttttca ttcctagatc
      481 caaattcaaa ggaaagtaaa tagaggaaaa aactatgctc agattagagg attttgtaga
      541 ggcaagagca agacttcaag gtgtgattag taaaacagag ctgatccata gccctatatt
      601 ttcagaagaa acaggcaatg agatttttat aaaaccagaa aacctccaat tgacgggctc
      661 ttttaagata aggggcgcat ataacaagat ggccaaacta agccaggagg agaaggaaaa
      721 aggagtgatt gcctcttcag caggcaacca cgcgcaaggg gtagcactag caggaaaaat
      781 gctgggcatc cagactctaa ttgttatgcc aaagtacact cctctgataa aggtggaagc
      841 aagcagaagc tatggggcta agctagtgct ctatggggaa gattatgatc aagcctataa
      901 aaaagcccta agcctaaaag aaaaaggcta taactttgtt cacccctttg atgatcaaga
      961 cgttatcgag ggccagggaa ccattgcact ggaagtgcta gaagaactag cagatgcaga
     1021 tattattatg gttcccctgg gtgggggtgg actcatatct ggaattgccg ctgcagccaa
     1081 attaaaaaat ccacaaataa aaattattgg agttgaagct gaaggggcag ccagcgccag
     1141 ctactcccta gcaagaaaga ggctagaaga attaccccga gtacatacca ttgcagatgg
     1201 ggcggccgta aaacgaattg gagagatccc ctttgcctat atacaaaaat acgtggatga
     1261 aattgtaacg gtttctgatt atgaactaat ggaagaattc ctaattcttg ttgaaaaaca
     1321 caaaattatt gcagagaatg cagccattct ttccattgcc gggctaaaaa agataaagga
     1381 aaaaaacaag aagatcgtct ccatcctaag tgggggaaat atcgatgttc tcaccatatc
     1441 ctccatgatt aataagggtc tggtttccag ggaaagaatt cttaagttct ccctaggact
     1501 cccggacaaa ccaggtcagt tggtcaatat ttcccagata ctggctgcac aaaatgccaa
     1561 tgttattaag ttggaccaca atcaatgtaa gaacctaaac cgttttcacc atgtagaact
     1621 acaagtcacg gtagaaacaa atggcgaaga acatataagc agcattatag aagccctata
     1681 taaagcagga tatcaaataa aaagactaaa ttaaggaaaa acgaaaggtg gtagacaatg
     1741 gagaaaaaat ggataaaggg ttcagaaata ttacttgaaa gccttagccg tttgggagtg
     1801 gatacaatat ttggttatcc agggggatcg gtgattccca tttacgacgc cctatatgat
     1861 tttaagaaaa taagccatta ttttgccaga cacgaacagg gagccgccca tgaagcagat
     1921 gggtatgcta gggctacagg caaggtggga gtctgctttg ctacatcagg gccgggggcc
     1981 accaacctag tgacaggaat tatgaccgcc catatggatt cggttcctct tatggccatt
     2041 accggccagg tgggaagtaa cctgctagga aaagatgcct ttcaggaaag tgatattgtt
     2101 gggattacca ttcccattac caagatgaac tacttggtca aagacattaa agaactgccc
     2161 aggattatca aagaagcgca ctttgttgca catacaggca ggccaggccc cgtcctaatt
     2221 gatctgccca aggatattca actagcagaa atcgagctag cagaattcga gaaattattt
     2281 acagaggata tccagctaga gggttatgta cccaactaca aggggcaccc caatcaaatt
     2341 aaaaaggcca ttgatctaat aaaaaaagcg gaaaaacccc taattatagc aggggctggg
     2401 attataaaat caaaggcctg tgcggagcta aaggaactag cacaaacaat ggatattcct
     2461 gtaaccacca ccctgctggg cctgggcagc tttccaagca gccataggct ctctctagga
     2521 atgctgggca tgcatggaac agttggtgcc aactttgcca cacaagaagc agatctagtt
     2581 atagccctag ggatgcgctt tgatgatcgg attgcaggag acccagaaaa cttttgtcat
     2641 cttgcccaga ttatccatat agatatcgat ccagctgaaa tcgaaaaaaa tcgaatggtt
     2701 gaaatcccca ttgtgggtga tttaaaggga gtattaaaag ccataaataa aaaattgact
     2761 ccaagaaaaa atacagactg ggtagaaaaa atcctaggat ggaaaaaaga gtatcccatg
     2821 gtttatggca aatcaagcga aaaactaatg ccccaggagg tcctggaaca tctggacaca
     2881 attgtccagg gccaggccat tgtatcaaca gatgttggac agcatcaaat gtgggtggcc
     2941 caatttctac agtttaccaa gcccaatacc atgctctcct caggaggagc cggaaccatg
     3001 ggatttggtc tgccagcggc cattggtgct caagtaggaa ggccagaaga actagttatt
     3061 gcagttgtag gagatggggg ctttcaaatg acctatcaag agctgatgct aatcaaacag
     3121 tatcagctgc cagttaaggt gctacttcta aacaattcct acctaggaat ggtaaggcag
     3181 tggcaggact tatttttaga taaacgttat tcatccgtta gcctagatat caatcctgat
     3241 tttgaaaaaa taggagaggc ctatggaatc aagacagtaa cccttaagac aaggggcgag
     3301 ctagaggacc agttagaggg actaatcaaa agtaaggagc cggtcattat acagtgcata
     3361 gtagaagggg aagcaaatgt ccttcctatg attccagccg gaaaatgcgc aaatcacatg
     3421 ataggtttaa ggggtgtaat ctaatggatc aatatgaaat attaattata gccaagaata
     3481 aggatggaat tatcggtaga atcctctccc tatttaataa aagaggctat tttgttagga
     3541 agatgacagc aggggctacc aatcgagcag gatatgcaag gctgaccttg acagtagaag
     3601 gagacgagct caccttggag caaatccaaa agcaagtcta caaaatcatt gatgttgtca
     3661 aggtcaagat atttccctct aagggagtca tccgccggga aatgatgcta atcaaggtca
     3721 aggccagcaa tgaaaccaga acccaaatta ttcaaatagc agatatttat agggggagtg
     3781 tactgg
//
LOCUS       DASRNA010000161         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_948618,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000161 DASRNA010000000
VERSION     DASRNA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948618"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1317
                     /locus_tag="VFD08_04415"
     CDS             70..1317
                     /locus_tag="VFD08_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK10297.1"
                     /translation="MITTERFEKVNIDKLVPYARNARTHSKEQILQLRASLREFGFIN
                     PVLSDKDFNIIAGHGRIMAAKEEGIAEVPCVFVEHLTEAQKRAYILADNRLALNAGWD
                     EEMLSVELSELQGVDFDISLLGFDDAELNKLLGDIDGVSDDDFDVEEELKKPAVTKLG
                     DLWLLGNHRLVCGDSTKEEAFNLLMDGKQANLTVTDPPYNVNYEGAAGKIKNDNMANE
                     AFYDFLLAAFTNMEAVMAKDASIYVFHADTEGLNFRKAFTEAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLFGWKKKGKHNWYSDRKQTTIWEFEKPKKNADHPTMKPVALVAYP
                     ILNSSLANCIVLDPFGGSGSTLIACEQTDRICHMIELDEKYCDVIVKRYIEQVGSNED
                     VFLLRDGIKTEYKAIESIFDKTA"
     gene            1407..2177
                     /locus_tag="VFD08_04420"
     CDS             1407..2177
                     /locus_tag="VFD08_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HZK10298.1"
                     /translation="MRINYNVTGPRRKKLVEAISKELETEAKYLAAPSFAYQVGDYDV
                     DRKGVLEGADNKDLVRKLFEVYNFKAEVEEYATSEPATESLETDGEAVGEGISPYRDY
                     EETPLYRPPSNEADSLIIELPMEGFTELALNNLRGLIKARESLIKKALGIECLPIITG
                     EETISFPWFSSSLEADEIKAYTNFVAKLYKMARKQKRVITTEKTVENEKYAFRCFLLR
                     LGFIGEKYKADRKILLKNLNGSSAFKDGLPANEEGEDC"
     gene            2171..2401
                     /locus_tag="VFD08_04425"
     CDS             2171..2401
                     /locus_tag="VFD08_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HZK10299.1"
                     /translation="MLKEIHPKTLEELKNSYPKGTRVKLIKMNDPYTKIEPGTLGTVT
                     GVDDIGTIHINWDCGSSLGIAFGEDKCQKLKE"
BASE COUNT          899 a          419 c          571 g          674 t
ORIGIN      
        1 gcttatatag gaaaccctca agacgatatt atggaggccc ttttatcagg aaggaaagga
       61 aggtaataga tgataacaac agaacgcttt gaaaaagtaa atatagacaa actagtgccc
      121 tatgctagaa acgctcgaac tcacagtaaa gagcaaatcc ttcagcttag ggcttcttta
      181 cgagaatttg gttttataaa ccctgtcttg tctgacaagg attttaatat cattgctggt
      241 catggcagaa taatggccgc taaagaagaa ggaatagccg aggtgccctg tgtatttgtc
      301 gagcatttaa ccgaagcaca aaaaagagca tatatcctag cagataatcg tctagcttta
      361 aatgccggtt gggatgagga aatgttatct gtggaattat cagaacttca aggtgtagat
      421 tttgacattt ccctattagg ttttgatgat gcagagttaa ataagcttct aggcgatatt
      481 gatggagtta gtgatgatga ttttgatgta gaagaagaac ttaaaaagcc ggctgtaaca
      541 aaactgggtg atctatggct cttgggaaac catcgcctag tttgtggtga ttccacaaag
      601 gaagaagcct ttaatctact aatggatgga aagcaagcaa accttacagt taccgatcct
      661 ccttacaacg tgaactacga aggggctgca ggtaaaatta aaaatgacaa tatggctaac
      721 gaagcttttt atgacttcct gcttgcagcg tttacgaaca tggaagcagt tatggcaaag
      781 gatgcttcta tttatgtatt tcatgcagat actgaagggc taaactttag aaaggctttc
      841 actgaggcag gtttttatct atctggtact tgcatctgga aaaagcaatc tttggtctta
      901 ggccgctccc cttatcaatg gcagcatgag ccagtgctct ttggttggaa gaagaaaggt
      961 aagcataact ggtattcaga tagaaagcaa actactatct gggaatttga aaagccaaaa
     1021 aagaacgcag accatccaac gatgaaacca gtagccctgg tggcttatcc tattttaaat
     1081 tccagcctag caaactgtat cgtgctagat ccctttggcg gatctggctc taccttaatt
     1141 gcctgtgagc aaacagatag aatctgccat atgattgagc ttgatgagaa atactgcgat
     1201 gtcatagtaa aaagatatat tgagcaggtt ggtagcaatg aagatgtttt tcttttaaga
     1261 gatgggatta aaacagaata taaagcaatt gaaagtattt ttgataaaac agcctaaaag
     1321 attttaatat aactcacata ctacttgcta tttacagcct ttagagtgat atatgtaact
     1381 accaaaaata gaaaggtggt atgggaatga gaattaacta taatgtgaca ggccccagaa
     1441 gaaaaaagct tgtagaagca ataagcaaag aattagaaac tgaagctaaa tacttagctg
     1501 caccctcctt tgcttaccaa gtgggagatt atgatgttga caggaaagga gttcttgagg
     1561 gagcagataa taaagaccta gttagaaaat tgtttgaggt gtataacttt aaggcagaag
     1621 ttgaagaata cgctacttca gaaccagcaa cagagagttt agaaactgac ggagaagcag
     1681 ttggggaagg aattagtccg tatagggact atgaagaaac tcctttatat aggcctccaa
     1741 gtaatgaagc agacagccta attattgaat tgccaatgga aggttttact gaactagccc
     1801 taaataatct aagggggcta ataaaggcaa gggaaagcct gataaaaaaa gcccttggga
     1861 tagaatgtct gccaattatt acaggtgaag aaacaattag ctttccttgg tttagtagta
     1921 gcctagaagc tgatgaaatc aaagcttata ccaactttgt agccaagctt tataagatgg
     1981 caagaaaaca aaaaagggta attactacgg agaaaactgt ggaaaatgag aagtacgcct
     2041 ttcgctgctt tcttttaagg cttggcttta taggggaaaa atataaagcc gatagaaaga
     2101 ttttactaaa gaatttaaat ggcagttccg cctttaaaga tgggctgcca gccaacgagg
     2161 agggagaaga ttgttaaaag aaatacaccc taaaacacta gaagagctta aaaactccta
     2221 tccaaaggga accagggtaa agcttataaa aatgaatgac ccttatacta agatagagcc
     2281 aggaactttg ggaactgtca ccggcgttga tgatatagga acaatccata tcaattggga
     2341 ctgtggctcc tctttaggca tagcctttgg agaggataaa tgccagaaac taaaagaata
     2401 aaatagatgt aaaatacttt gaaatacttg aaaatatgac ttgctatata tgccttttag
     2461 agtgatatat gtacataccc caaggggaat acacacttta aaaggagcga gcaagatgct
     2521 aaaagcacgg tttggaatcg aaatcgaaat gacagggatc acc
//
LOCUS       DASRNA010000162         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_424672,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000162 DASRNA010000000
VERSION     DASRNA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424672"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..704
                     /locus_tag="VFD08_04430"
     CDS             126..704
                     /locus_tag="VFD08_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HZK10300.1"
                     /translation="MAKDGTLRGGRRVRAGDKPQPLTEKITKGKTAKILEVPDFQPES
                     ILEAEDLEVDADFDGEEMPSPSDYLSSKQKDGKTLGADDLFIETWKWLRNRGCEKFVN
                     PRLIEAYAQAFTRYIQCEEAISTYGLLGKHPTTGGAMASPFVQMSQSFQKQANLLWYE
                     IFDIVKQNCTTAFVGNPQDDIMEALLSGRKGR"
     gene            710..1951
                     /locus_tag="VFD08_04435"
     CDS             710..1951
                     /locus_tag="VFD08_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK10301.1"
                     /translation="MISTERFEKVNIDKLVPYARNARTHSKEQILQLRASLREFGFVN
                     PVIVDKDLNIIAGHGRILAAKEEGVKEVPCVVVEHLTEAQKRAYILTDNRLALNAGWD
                     AEMLSVELADLQGVDFDISLLGFDDAELNKLLGDIEDVQDDDFDVDAELKNPSITKLG
                     DLWLLGNHRLVCGDSTKEETFNLLMDEKQANLTVTDPPYNVNYEGAAGKIKNDNMGDE
                     AFYDFLLAAFKNSEAVMSKDASIYIFHADTEGLNFRKAFSDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLFGWKKKGRHLWYSDRKQTTIWEFEKPKRNADHPTMKPVALVAYP
                     ILNSSLTNCIVLDPFGGSGSTLIACEQTDRICHMIELEEKFCDVIVKRYIEQVGSEDG
                     VFLVRDGNEYGFQDVQKLVEQ"
     gene            2047..>2459
                     /locus_tag="VFD08_04440"
     CDS             2047..>2459
                     /locus_tag="VFD08_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HZK10302.1"
                     /translation="MKINYNVTGPKRKALVEAITQELGIPVKYLGAPTFAYEVGNYII
                     DKSGVLEGKDNPGLVADLLEIHDFKAATEEYDTPFPEEESTSMIIELPRADFTDVALE
                     NLKGLVESKETLIKNALGIDSVLVMVGEETISFSW"
BASE COUNT          777 a          449 c          580 g          653 t
ORIGIN      
        1 gtgaccggtg ggggcggtct gatctctaaa accttttaaa gcggacagcg gcgtggggca
       61 acgcgtgaaa aaattcaggt tcaaaggggg gattaaggcc tagcagcgaa aggaggtgcc
      121 gacaggtggc aaaagacgga acgctaaggg gtggtaggcg ggttagagcc ggagacaaac
      181 cccagcccct tacagagaaa ataactaaag gtaaaacagc aaaaatacta gaagttccgg
      241 attttcagcc ggagtcgatt ttggaagctg aggacctgga agttgatgct gattttgatg
      301 gagaagaaat gccatcgcca agcgactacc ttagttcaaa gcaaaaggat ggcaaaaccc
      361 taggagcaga tgacctattt atagaaactt ggaaatggct tagaaatcgt ggttgtgaaa
      421 aatttgtaaa tccaaggctt attgaagctt atgctcaggc ctttacaaga tatattcagt
      481 gcgaagaagc tattagcact tatggtctac tcggaaaaca ccccacaact ggtggagcga
      541 tggccagccc ctttgtccag atgagccagt catttcaaaa acaagctaat ctcttatggt
      601 atgagatttt cgacatagta aaacaaaact gcaccacagc ttttgtgggc aatccacagg
      661 acgatattat ggaggccctt ttatcaggaa gaaaaggacg gtgatataaa tgatttcaac
      721 tgaaagattt gagaaagtaa acatagataa gctggtgcct tatgccagaa atgcccgcac
      781 tcatagcaag gagcagatac ttcagcttag agcatctctt agggaatttg gatttgtgaa
      841 tccggttatt gtggataagg atttaaacat tatagcaggc catggtagaa ttctggctgc
      901 taaagaagaa ggtgtcaaag aagttccctg tgtagttgta gaacatctaa cagaagcaca
      961 aaagagagca tatatcctta cggataatcg tctggcctta aacgccggct gggatgcaga
     1021 aatgctttcc gtggaactag cagatctgca aggtgtggat tttgacatct cccttcttgg
     1081 ttttgatgat gcggagctaa acaaactctt aggtgatata gaggatgtcc aagatgatga
     1141 tttcgatgta gacgcagaac ttaaaaaccc atctattacc aaactggggg atctatggct
     1201 attaggaaat catcgtctag tttgtggcga ttccacaaaa gaagaaactt tcaatctgct
     1261 tatggatgaa aaacaggcta atcttacagt tacagaccct ccatataatg ttaattatga
     1321 aggtgctgca ggtaaaatta aaaacgataa catgggagat gaagcattct atgacttcct
     1381 gcttgcggca tttaaaaata gcgaagcagt gatgtccaag gatgcttcta tttatatctt
     1441 ccatgcagat accgaaggtt taaattttag aaaggctttc tcagacgcgg ggttttatct
     1501 ttctggcact tgcatttgga aaaaacaatc cttagttctt ggccgttccc cttaccaatg
     1561 gcaacatgag cctgtgctct ttggctggaa aaagaaagga aggcatctct ggtattcaga
     1621 tcgcaaacag acaacgattt gggaatttga aaagccaaaa aggaatgcag atcacccaac
     1681 catgaagcca gtagccctag tggcctaccc cattctaaat tcaagcctaa ctaactgtat
     1741 cgtgcttgat ccctttggtg gcagcggctc tactcttatt gcttgcgagc aaactgatag
     1801 gatttgccac atgatagagc ttgaagaaaa gttttgcgat gtcattgtta aaagatatat
     1861 tgagcaggtt ggaagcgagg acggtgtatt tcttgtgcgt gatggtaacg aatatgggtt
     1921 tcaggatgtt caaaaattag tagaacaata aatagttatt tttttattaa ttactcacat
     1981 accacttgct atttacacct ttctaagtga tatatgttac tagcaaaaag aaaggtggtg
     2041 tggaaaatga agattaatta caatgttaca ggccctaagc gaaaagcact ggtggaagca
     2101 ataacccagg aactcggtat tcctgtaaag tacctgggtg ctcctacttt cgcttatgag
     2161 gtgggaaatt acatcattga taagagcggg gtacttgaag gaaaagataa tcctggatta
     2221 gttgctgacc ttttagaaat acatgatttt aaggcggcaa ctgaagaata cgatacccct
     2281 tttccagaag aagaatccac ttccatgatt attgaattgc caagagcgga ttttaccgat
     2341 gtagcccttg aaaaccttaa aggcttagta gaaagcaagg agactctcat taaaaatgct
     2401 cttggcatag attctgttct ggtaatggtt ggcgaagaaa ctatcagctt ctcttggtt
//
LOCUS       DASRNA010000163         5068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_796911,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000163 DASRNA010000000
VERSION     DASRNA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796911"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..873)
                     /locus_tag="VFD08_04445"
     CDS             complement(193..873)
                     /locus_tag="VFD08_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK10303.1"
                     /translation="MDKLLIVDDEPILLKGLKFSLEQEGYLIDTAVDGEEALEKALEN
                     KYDLLVLDVMLPKMDGIEVCRRIRETSMVPIIMLTAKGDDESKISGLEAGADDYMGKP
                     FNILELKARIRAVLRRVKPKNMASSSIISFDKFSVNFFGRKVIFDDREVTLTAKEFDL
                     LILFVLNEGTVFSREELLEKIWGYEYFGDVRTVDVHVRRLREKVEADSSHPEFIMTKW
                     GTGYYFKQ"
     gene            complement(866..2722)
                     /gene="selB"
                     /locus_tag="VFD08_04450"
     CDS             complement(866..2722)
                     /gene="selB"
                     /locus_tag="VFD08_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZK10304.1"
                     /translation="MSRFVVGTAGHIDHGKTTLIKALTGIPTDRLAEEQKRGISIVSG
                     YAYLNYKEEKISIVDVPGHERFIKNMLAGVSGIDLVLLMVAADEGVMPQTREHFDILR
                     LLGINRGLILMTKCDLADEEMQELVEMDIEELVEDTSFENFPILRVSAKSGEGLDSLK
                     EYIFSEYEKSKEEPPYYPRLFIDRVFKGKGIGDIVTGTLEGADLAIDTPLALYPGQKD
                     FNIRSIQSHEESTKLAQVHSRVALVLQGEDKDALEPGKALAPKDKYHETRSLIVKVEA
                     LKEVDERIKSGEEYKVYFGSDEFFAKLHAIKDNFYELELDRAMMAFFGQRGVLRQMSP
                     VITLGGLEVLDSNPLMGKRNKLESVKHLTNNDFSSRLNYIGWKYSQGFTLEDLEREMV
                     LQKEVLEEKIALEDIELMEQGKRFYTQDSLWRQQDELIRNLEKLCQSDPLKDYWDKEV
                     LRSRYYRQMDRSLFEEMLKKAHEEEKITLSGNKVKPFSYKLVLSEEQLGIKEKILKKF
                     DIKSYEIPTLSEVKSKLGKEELALLEYLIHNGELISINSEYFMKKDMVYCLKNDIIEL
                     GLGNRYIEIADVREALGLTRKYILPYLEYFDRTGVTQRVENARILTKEYENG"
     gene            complement(2719..4065)
                     /gene="selA"
                     /locus_tag="VFD08_04455"
     CDS             complement(2719..4065)
                     /gene="selA"
                     /locus_tag="VFD08_04455"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZK10305.1"
                     /translation="MNQLRQIPNVHELLEDPALKGVLEKYKRSYVSFLLKEQLDRLRK
                     EILSGKEVEISKKNILDELILEVKQPYALREVLNATGIVLHTNLGRAPLSKESFERLE
                     RLSRSYSNLEYDLEKGKRGSRYDHLRRHLQYLTGAEDAHIVNNNAAAVFLILSTFSKD
                     KEVLLSRGEMIEIGESFRIPEIIKNSGARLVEVGTTNRTHLWDYEKAIGENTGMIMKV
                     HPSNYYIGGFSQEVEARELAKLAKQHNIPLYEDVGSGSLLEEGLYREKNSVREALEHV
                     DLLSFSGDKLLGGPQAGIILGKKADIRSLKKNQLTRVLRVDKLILAALEATLLSYTKK
                     NFDLPTYFFLNRTYEELLLRSEDFLEDLKKAGIEGEIKEGKSLVGGGALPRQRIPSPQ
                     IVLFVDKNLEKVNLRLRRSDPAIVGVLSEGQLLIDLRCIYPQDEKILKEALKEVLL"
     gene            complement(4067..5050)
                     /gene="selD"
                     /locus_tag="VFD08_04460"
                     /pseudo
     CDS             complement(4067..5050)
                     /gene="selD"
                     /locus_tag="VFD08_04460"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
BASE COUNT         1447 a         1136 c          817 g         1668 t
ORIGIN      
        1 ggattaatag actcaaggga ttttagattg ttggcaactt cttgagatat ctcaaaaaga
       61 gggtctaccc tctttttgat ttccgttctt gataaagaaa tgtaaagaaa attatgaatg
      121 gcaaacaagc ctactattaa cagcaaaaaa taggtggaaa taatcctcca ccctaatttt
      181 gtgctaattt tatcactgtt tgaagtaata ccccgtaccc catttcgtca tgataaattc
      241 cggatgtgaa ctgtcagctt ccactttttc acgaagtcgt cttacgtgta cgtctactgt
      301 tctaacatca ccaaagtact cataacccca tattttttct aagagttctt cccgactaaa
      361 tacagttcct tcattaagaa caaaaagaat caaaagatca aattctttgg ctgttaaggt
      421 tacttctcga tcatcgaaaa ttacttttct gccaaagaaa ttcacactga atttatcaaa
      481 tgagataata gaagaagaag ccatgttttt aggtttaact cttcttaaga cggcgcgaat
      541 ccttgctttt agctctagaa tgttaaaggg cttgcccata tagtcatcgg ctcctgcttc
      601 caaaccagat attttagact catcatctcc tttggcagta agcataataa taggaaccat
      661 acttgtttca cgtatgcgcc tgcatacttc aatcccatcc atttttggaa gcataacatc
      721 caaaaccaat aggtcatatt tattttctaa agctttttct agggcttctt ccccatcaac
      781 agctgtatca attaaatagc cttcttgttc taagctgaat ttaagtcctt taagtaaaat
      841 tggctcatca tcaacaatta aaagtttatc cattttcata ctcctttgtt aaaattcgtg
      901 cattttctac acgctgggtt actcctgtac gatcaaaata ttcaagatag ggaaggatat
      961 attttctagt taaacctaaa gcttctcgaa catctgcaat ctctatgtac ctattaccca
     1021 gacctagctc gattatatca ttttttaaac aatataccat atcttttttc ataaaatact
     1081 cagaatttat agaaataagt tctccattat gaatcaaata ctcaagtaga gctagttctt
     1141 ccttcccaag cttgctttta acttcactta gggtgggtat ctcataggat tttatatcaa
     1201 acttttttaa gatcttttct tttataccca attgttcttc acttagcact aatttatagg
     1261 aaaaaggttt gactttattt cccgaaagcg tgattttttc ttcctcatgt gcctttttaa
     1321 gcatttcttc aaataggcta cgatccatct gcctatagta gcggctgcgt aatacctctt
     1381 tatcccaata atcctttaga ggatcagatt ggcagagctt ttcaaggttt ctaatcagct
     1441 catcttgttg tcgccaaaga ctatcctgtg tataaaagcg ctttccttgt tccataagct
     1501 ctatatcttc tagggctatt ttttcttcta ggacttcctt ttgcaagacc atctctcgct
     1561 ctaaatcttc tagcgtaaaa ccctgggaat acttccaacc aatatagttt aatcgagaag
     1621 aaaaatcgtt atttgttaga tgtttaacag attctagttt atttcttttt cccattaaag
     1681 gattgctatc tagaacttct agcccaccta gggtaatcac tgggctcatc tggcgtagaa
     1741 ctcctctttg tccgaaaaaa gccatcatag ccctatccag ttctaattca tagaagttat
     1801 ccttaatggc atgtagtttg gcaaaaaact catcggaacc aaaataaacc ttgtattcct
     1861 ccccactttt aatgcgctca tcaacttcct tcaaggcttc cacttttaca ataaggcttc
     1921 ttgtttcatg gtacttatct ttaggggcca aagccttacc cggctctaag gcgtctttat
     1981 cttcgccctg caagacaagg gcaacccgag aatggacctg ggctaactta gtggactcct
     2041 catggctttg gatggagcga atgttaaaat ccttctgtcc aggataaagg gccagagggg
     2101 tgtcaattgc taaatctgct ccctctaaag tacctgttac aatatcacca attcccttgc
     2161 ctttaaagac cctatcaata aaaagacgag gataataagg gggctcttct ttgctttttt
     2221 catattctga gaaaatatat tcctttagag aatctaggcc ctccccactt tttgcactta
     2281 ccctaagaat agggaagttt tcaaaggaag tgtcctctac taattcttca atatccatct
     2341 caacaagttc ttgcatttct tcatcagcaa gatcacactt ggtcataagg ataaggcccc
     2401 tattaatacc taaaagcctt aaaatatcaa aatgttccct tgtttggggc ataactcctt
     2461 catcagctgc taccattaag aggacaagat caataccact gacccctgct agcatatttt
     2521 taataaaacg ttcatgacca ggaacatcta caatcgagat tttttcctct ttataattta
     2581 aataagcata gccactaaca atacttatgc ctcttttttg ttcttcggcc aagcggtccg
     2641 tgggaattcc tgtaagggct ttaattagtg ttgtttttcc atggtcaata tgcccggctg
     2701 tgcctacgac aaatcggctc ataaaagaac ctccttcaag gcttccttaa ggattttttc
     2761 atcttgggga taaatgcaac gaagatctat aagaagttgc ccttcactta ggactcctac
     2821 aatagcagga tcgcttctac gaaggcgaag gtttactttt tcgaggtttt tatctacaaa
     2881 aagtacaatc tgaggactag gaattctttg tcttggcaga gctcctcctc ctacaagact
     2941 ttttccttcc tttatctctc cttctatccc agcttttttt aggtcttcca aaaagtcttc
     3001 gcttcgcaaa aggagctcct cataagttct attaaggaaa aaataggtag gaagatcaaa
     3061 atttttctta gtataggaca aaagagtcgc ctcaagagct gctaagataa gcttgtccac
     3121 cctaagaaca cgagtcaatt ggtttttctt aagagaccta atatcagctt tttttcctaa
     3181 aataatgccc gcctgagggc ctccaagtag tttgtctcca ctaaaactta aaaggtcaac
     3241 atgttccagg gcctctctta ccgagttttt ctccctatag agaccttctt ctagaagaga
     3301 accacttcct acatcttcat aaagaggtat attgtgttgt ttagccaatt tagccagttc
     3361 tcttgcttcc acttcttggc taaaacctcc aatatagtag ttacttggat gcaccttcat
     3421 aatcatccct gtgttttcgc cgatggcttt ttcataatcc catagatgag ttctattggt
     3481 agttccaact tcaactagcc ttgctccaga gtttttaatt atttctggaa ttcgaaagga
     3541 ttcgccgatt tctatcatct ctccccttga taaaagtact tctttatcct ttgaaaaggt
     3601 acttaggata agaaaaactg cagcggcatt gttatttact atatgggcat cttctgcccc
     3661 tgttagatat tgcaaatgtc tacgaagatg atcgtagcgg ctgcctcttt ttcctttttc
     3721 caaatcatat tctaggttgg aatatgaacg agatagacgc tccagtcgct caaagctttc
     3781 tttagaaagg ggtgccctac ccaaattagt atggaggaca atccctgttg cattaaggac
     3841 ttctcttagg gcatagggct gcttaacttc taaaataagc tcatcaagaa tattcttttt
     3901 ggaaatttct acttcttttc cactaagaat ctcttttcta agtctatcta gttgttcctt
     3961 aagaagaaaa cttacatagc ttctcttata cttttccagg acccccttta gggctggatc
     4021 ttctagaagt tcgtgaacat tgggtatctg acgtagttgg ttcattttag ccccttttca
     4081 catatatttg tttttccttt ttttcaacaa tttcaccgat aatggccact ggtgttttta
     4141 ctttttcttg aagtttatca accaccctat cggctatagc cgcatcaaca ctgagtaaaa
     4201 gccccccact agtctgggca tcaaaaagcg gtaatacttc ttgcccagta aaaccatcta
     4261 aattacaacg accctcatag tggtttttat tgccaaaggt tcctccagga acagctccag
     4321 cctcaacaat ttcttccact cctggcaaaa aaggaatggc atcataatca aggtaggctg
     4381 aagtatcact agcctccatc atctcaagta ggtggccaat tagtccaaaa ccagtaacat
     4441 ctgttgcaca atgggccttg ctaccctcca tggcttcaag ggcgtattta tttaaatact
     4501 gcatgctaaa gatagcttcc tctgccatta tttcactggc aattccagat tttatcccgg
     4561 ttgttacaat acctgtgcct aaggcctttg ttagaattag tttatcccct acctttgctc
     4621 caccattaag aagaatatcc tcattattta caaagccgat tacactaaga ccataggtgg
     4681 gctcactgct tttaatggag tgacctccta ctagtagggc tccggcttct tttactttat
     4741 cggctccccc tctaagaatt tctctaagaa tttcaagaga aagttcactg ggaaaaccaa
     4801 caatatttag ggcaagttta ggttttcctc ccatagcata gacatcactc aagctatttg
     4861 ctgcagcaat ggcaccaaaa gtatagggat catcgacaat gggcgtaaaa aaatcaagag
     4921 tcgtaataat cgaccttttt tcatctaatt tataaacagc tgcatcttca ctataatcaa
     4981 aacctgagat tagcatttca tcatgaaact caggtagact acttaatata tctgataaac
     5041 tatcaggagc aagtttagcc gctcagcc
//
LOCUS       DASRNA010000164         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1096757,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000164 DASRNA010000000
VERSION     DASRNA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096757"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(479..1033)
                     /locus_tag="VFD08_04465"
     CDS             complement(479..1033)
                     /locus_tag="VFD08_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10306.1"
                     /translation="MKINKRLILLLITILMVFTITTGCGADAGAEKADNIKENQPATQ
                     LDDSSQEFLCSGKNDTCPNKTRGPSNYFCEECDADQDNVEDVKESPAVEEGSSQEYLC
                     MGKSDTCPNKTSGPSSYFCEECDADQDNVEDVKESPAAEEGASQEYLCRGKNDTCPNK
                     TGGPSNYFCDECDADHDNIEDIKQ"
     gene            complement(1169..1939)
                     /locus_tag="VFD08_04470"
     CDS             complement(1169..1939)
                     /locus_tag="VFD08_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZK10307.1"
                     /translation="MENKLQGSMEAGKKTVGTFLSFADADFAECIGLAGLDYVIVDTE
                     HGPNDVEQTVSLLRASESRGATALVRVKDSSRSSILKMLDIGAMGLVIPNIRSIEEVK
                     DIIKYGKYHPLGQRGFTTARAAGYGFADYSADIEEYMDLSNKKTMLIPQCETLESLAA
                     IEEIASLAGVDGIFVGPFDLSQAMGKMGQFDSEEFQEALERILKACHAAEIPAFIFAT
                     SPDMAKKYFQMGFDSVALSIDIWVFTNAYKDLLKKTLG"
     gene            complement(2038..3252)
                     /gene="trpB"
                     /locus_tag="VFD08_04475"
     CDS             complement(2038..3252)
                     /gene="trpB"
                     /locus_tag="VFD08_04475"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZK10308.1"
                     /translation="MKFDFTNYLKKYPDKQGYFGEYGGIHLPENLERALREVEEAYYR
                     IAHSAEFISELRRIRKEFQGRPTPVYHCKNLSQELGTSQIYLKREDLNHTGAHKLNHC
                     MGEGLLAKYMGMKKLIAETGAGQHGVALATAAAFFGLECEIHMGEVDIKKQASNVTKM
                     KLLGAKIIPVTHGLRTLKEAVDSAFDAYMKDYKNSIYCIGSVVGPHPFPMIVRDFQSI
                     VGIEARSQFKEMTGYLPDAVVAAVGGGSNSIGMFSGFLEDPVDLYGIEPLGKGSQLGD
                     HAASLMYGEKGIMHGFESIMLKNEKGEPAPVYSLASGLDYPSSGPEHAFLRDMGRVNY
                     DVISDVEAVDAFFRLSRMEGIIPAIESAHALAYALKLAKKEDNPGFILVNLSGRGDKD
                     IDHILEAYPNGI"
BASE COUNT         1013 a          894 c          591 g         1300 t
ORIGIN      
        1 caatacatct tctaataaat gccaactaac aactttttca tttatttgtc agggagttaa
       61 attggaattg aaaaaggctg accttcttta tcagccaagt ttattttatg atttttccaa
      121 atggagtttt tcttatacca agccaaatac cttgctttaa agggaaataa attgatcttc
      181 attcctagat atttatgact aaatgaagta tccctataca tttcttttaa gttttcatat
      241 gctggaggtt tttagctcct tgtgctctta gttatgggcc tatttaccct aaataccctc
      301 tactcctcta aaaatccccc gtgctccttg cccagagact tcaagagtat caattaattt
      361 aaataatgaa aaagtagctg agcgaatttc tcgactaagc tacttttcat tattcactag
      421 attgtttttt attagatttc ttactaaaag tgtcactcca agagcaaggg ttttaatttt
      481 attgttttat gtcttcaata ttatcatgat cagcatcaca ctcatcacaa aagtagttag
      541 agggaccccc tgttttattg gggcaggtat catttttacc cctacataaa tattcttggc
      601 tagcaccttc ttctgccgct ggagattctt ttacatcttc tacattgtct tgatcagcat
      661 cacactcttc acaaaagtag ctagagggac cgcttgtttt attggggcag gtatcactct
      721 tgcccatgca taaatattct tggctagaac cttcttctac tgctggggac tcttttacat
      781 cttctacatt gtcttgatcg gcatcacact cttcacaaaa atagttagaa ggaccccttg
      841 ttttattggg gcaggtatca ttcttaccac tacataaaaa ctcctgggaa gaatcatcca
      901 gttgggtagc tggctgattt tcctttatat tatcagcctt ctctgcccct gcatctgcac
      961 cacatccagt ggtgatagta aaaaccatca aaatagtgat gagaagtagt atcagtcttt
     1021 tatttatctt catatcattc ccttcaaagt acttgtcctc ttgtatctct agtagccacc
     1081 tattaaaata agtgactcta aaacttgcat caagtcctta aaaacaaatc ctgcctttat
     1141 aaaactatgt tttctaatag aacctctatt aacccagtgt ctttttaagc aaatccttat
     1201 aagcattagt aaagacccaa atatcaatag aaagggcaac actatcaaag cccatttgga
     1261 aatatttttt tgccatatca ggactcgtgg caaagataaa tgccggaatt tcagctgcat
     1321 ggcaggcctt taagattcgc tctaaggcct cttgaaattc ctcgctatca aactgtccca
     1381 ttttacccat tgcttgagat aggtcaaagg gacctacaaa tataccatct acccctgcta
     1441 gggatgcaat ctcctcaatt gccgctaggg actctagggt ctcacattgt ggaattagca
     1501 tggtcttttt attgcttaga tccatatatt cttctatatc tgctgagtaa tcggcaaagc
     1561 catatccagc ggccctggct gtagtaaagc ctctttggcc taggggatgg tattttccat
     1621 atttaataat atcttttact tcttctattg agcggatatt tggtattacc aagcccatgg
     1681 ccccaatatc aagcatcttt aggatagaag acctactgct atcttttact ctaactaggg
     1741 ctgttgctcc ccggctttca cttgcacgca agagactcac cgtttgctcc acatcattag
     1801 gtccatgttc tgtatctaca attacataat ctaggcccgc taagcctatg cactcggcaa
     1861 aatcagcatc ggcaaaactt agaaatgttc ctactgtttt ctttcctgcc tccatcgacc
     1921 cttgtaattt attttccatg acttagtcta ctccttttat ctttcttata gtaattatac
     1981 catcacttaa gatattcaaa gaattttttc ttagtcagta gaaaaaaagc tttcttttta
     2041 gatcccattt ggataggcct ctaagatatg atctatatcc ttgtctcctc taccacttaa
     2101 gttaaccaaa ataaagccgg gattatcttc tttttttgct agtttcagcg cataggctag
     2161 ggcatgggca ctttctatag caggtatgat tccttccatt ctagatagtc taaagaaggc
     2221 atctaccgct tccacatcgg aaatcacatc ataatttact ctacccatat ctcttaaaaa
     2281 ggcgtgctca ggcccactag agggatagtc taagccgctg gctaaggagt agaccggggc
     2341 aggttctccc ttttcgtttt ttaacataat tgattcaaag ccatgcataa tgcctttttc
     2401 tccatacatt aaggaggctg catgatctcc aagttgactt ccttttccta ggggttcaat
     2461 gccatataag tccacagggt cttctaaaaa cccgctaaac attccaattg aattagatcc
     2521 tcctcctaca gccgccacga ctgcatcagg taaatagcct gtcatctcct taaattgaga
     2581 tctagcttct atgccaacaa ttgactgaaa atcccttact atcattggaa aggggtgggg
     2641 gcccactaca gaaccaatac agtaaataga atttttataa tctttcatat aggcgtcaaa
     2701 ggcagaatct acagcttctt ttaaggtcct caagccatgg gtaacaggga tgatcttggc
     2761 ccctaggagc ttcattttcg ttacattact tgcttgcttt ttgatatcca cttcacccat
     2821 atgaatttcg cattctagcc caaaaaatgc tgccgccgta gcaagagcaa ctccatgctg
     2881 gcctgctcct gtttctgcga ttagtttttt catgcccata tactttgcta aaagaccctc
     2941 ccccatacaa tggttgagct tgtgagcacc tgtgtgattt agatcctctc tttttaaata
     3001 tatttggcta gttcctagtt cttgcgatag gtttttgcag tggtacaccg gcgttggcct
     3061 accttgaaat tcttttcgaa ttcttctgag ttcacttata aactcggccg aatgggcaat
     3121 cctataatag gcttcttcta cttcccttag agccctttct aggttttctg gtagatgtat
     3181 tcccccatac tctccaaagt agccctgctt gtctggatat ttctttaaat aattggtaaa
     3241 atcaaatttc attattctct cctctttata ggtatttgac tagctaagaa actgccccta
     3301 tcttgtatct tttccctgtg tcatttgcca agacaattcc ccctattgcc ttatttttca
     3361 ttggtttttt tctttctaca aagtttcact tttcttccaa taaaaaaaca cttatcctct
     3421 aaaggacaag cgttgcctgc ggtaccacct taattaatct ttgcataaag attctcttat
     3481 caggatgcca acacatccct agcctataac gcggctccgt tttagttact cggtttccct
     3541 ttcgcctcac cctcaaaggc ccatcttcaa gatcttactc tatttggctc tcaccaaccc
     3601 aaactcgctt taagattatc ttcttgagaa tcccttctca tcggtttttc tattattcca
     3661 atatactacc acattccttt gtttttatca atccttcatc caaatatatt tatccgcttt
     3721 tacttttctc ctattgactt ctccccataa aactcctaac tccaggcctt ttatcataaa
     3781 aagtccttga gatcaatc
//
LOCUS       DASRNA010000165        15688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1097096,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000165 DASRNA010000000
VERSION     DASRNA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15688
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097096"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..736)
                     /gene="coaE"
                     /locus_tag="VFD08_04480"
     CDS             complement(161..736)
                     /gene="coaE"
                     /locus_tag="VFD08_04480"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZK10309.1"
                     /translation="MKFNYGITGDIASGKSTLSSYLKELGFMVIDADLIAREVMEPGR
                     EGYFLVKKAFPEAFKEEKLQREILASIIYKDKKRRKELDKLLHPLIIKIMFKRGQGHI
                     AFHDAPLLFESGMDKYLNKVLYLAVDKKTQLDRLIKRDGISKEEALKKMSAFDYPREE
                     KLKKSYIIENNKSKEELYEKVRTFLQEENLL"
     gene            complement(826..3195)
                     /locus_tag="VFD08_04485"
     CDS             complement(826..3195)
                     /locus_tag="VFD08_04485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZK10310.1"
                     /translation="MNKLLIVDGNSLVNRAYYALPPLRTEEGFFTGGIHGFLRMLDNM
                     LERENPSHLIITFDTKEKTFRHLKYDGYKANRKGMPDELAMQMPVLKEILDALGLARI
                     ELPGYEADDLMGTLSAKASEEGFHSVILTGDKDTLQLVNESVTVAIPKKGISTLISYD
                     EKEVVEELGLYPNQVIDEMGLRGDPSDNIPGVKGVGAKTALKLLEGGRHLEDVYKDPG
                     KVTPRIKKLLEEGRELAYLSRDLAKIDLDAPVEFEEAFFSYSGMNYQSAREILEKYEL
                     RSFLKNIEVEEEVNYEEDVEILHTRPPLEGSVVYWNYKDFHGVKEKGKYYVFTGDIDF
                     SGAKNLISHNLKDLYLKEIPLPYKKDDIMISHYLLHPDLKSNIAIDLSGLQEPSEMDD
                     FFMAKMLDTIEKLNIQQLEALKTKGMLSLYNEVEIPLVEVLASIEKEGFNVDLEELDK
                     EDELISGRLKEVEERIFSYVEEPFNVRSPKQLGVVLFEELGLPVIKRTKTGYSTNKEV
                     LDKLQGRHPIIEDILHYRALAKIKGTYIDGLRKVIDQDGKIHTSLNQTVTVTGRLSST
                     EPNLQNIPIRYEEGRRIRKIFVPRDKNYLLLSADYSQIELRVLAHFSEDPVMLEGYKK
                     DLDIHKLTASVVFDTPLEEVTSHQRREAKAVNFGIVYGISDFGLSENIDIPIAAAKDY
                     IEKYFENYPSIKAYLDSQVDFAKEHGYVKTLLGRIREIPQIHSKNFHMRSFAQRTAMN
                     TPIQGSAADIIKIAMIQVWRKLKERKLESKIILQVHDELNFRCKKTRAG"
     gene            complement(3192..4016)
                     /locus_tag="VFD08_04490"
     CDS             complement(3192..4016)
                     /locus_tag="VFD08_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK10311.1"
                     /translation="MKSCEWIEQSIQKKYRHKLWTPFIRAIKEYQLIEEGDQVAVAIS
                     GGKDSFLLAKLFQELYRHGNRNFELSFIAMDPGYAPQHRDHLEKMADYLNIPIKIYSR
                     NVFEVSLKLSEKPCFLCARMRRGALYDLAKKEGCNKLALGHHYDDVIETILLNLLQGG
                     ITMAMMPKLHAQNFPGMELIRPLYYLREKDILGWQEEAGLSFLDCACPVAEKQNQSSR
                     QKIKKLLPILKEDFPRVEESIFASMKHVHLGSVLGTVDKGKKKTFLEKYKDERMDL"
     gene            4064..4762
                     /locus_tag="VFD08_04495"
     CDS             4064..4762
                     /locus_tag="VFD08_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZK10312.1"
                     /translation="MNPFIRTQALLEGDFKKIENASVAIIGLGGVGSHAAMALSRMGI
                     RKLILMDPDRIKISNFNRHAQGYTRYLDHNKAEAMKEEILSFRPMDILVIPKLYDLSE
                     EDNLFSQDFDILIDCIDVMTFKIRLIEACQQRKIPFISSLGTGNRLQPTELEITSIYK
                     TQGCPLARVLRKEARKRQIENFPVIISKEKARNIVLKEKEGSRHLPASSYFTPAAAGQ
                     LLAYWAIKSLLDKT"
     gene            4845..5822
                     /locus_tag="VFD08_04500"
     CDS             4845..5822
                     /locus_tag="VFD08_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK10313.1"
                     /translation="MKKFLSIAMIALLVFTLAACTDEASETESAQEETAETQEEEEEV
                     VEEALVIETLKVQITPSKDAARLDAQRKPLQDMLEEKLAMPVEVSIGTDYSATIEAMR
                     SGQIHVGFLAPTQYVLAHGQDAADVILKSLRYDVDDNGDKLEDAPLVDNFRSQLVAAT
                     DSPINSVQDLKGKTIAVASFTSTSGFVWPANLLADSGLDPQADVEWVNVGGHDLAIQA
                     VYNGETDAAFTFKDARFVLKEEHPDVYDKVRFVLDTEGIPNDTISVIPQMDQELRDRI
                     ADAFLSIVDEEEGLEVFRLIYEWEGFAKAQDSDYDIVRTYLQRQEEWDF"
     gene            5867..6649
                     /gene="phnC"
                     /locus_tag="VFD08_04505"
     CDS             5867..6649
                     /gene="phnC"
                     /locus_tag="VFD08_04505"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HZK10314.1"
                     /translation="MPQLEIKNISKVYPNGVRALDGVSFQVNKGEFVSIIGLSGAGKS
                     TLLRSINKLIEMSGGEIIFEGKDIASAKGKDLRLIRRDMGMIFQSFNLVKRSKVITNV
                     LAGRIAYHKPWRTILGLFPKEDKAIAFEALNRVNILEKAYSRADQLSGGQMQRVAIAR
                     SLAQEPKLLLADEPVASLDPLTTIQVMDDLKRINRELGITTLVNLHHVDLALKYSTRV
                     IGLREGKLVFDGPANNVDDEILKQIYGRELLSNEVFGEDNEK"
     gene            6639..7439
                     /gene="phnE"
                     /locus_tag="VFD08_04510"
     CDS             6639..7439
                     /gene="phnE"
                     /locus_tag="VFD08_04510"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HZK10315.1"
                     /translation="MKNDDKLAPSLPKKPAELKRNLSILLFLSIIYASGSFTNFSLSE
                     LLGNLGEAGDLLGRMFLPPDFSYARRLFLPMVETLQISFLGTVIGSIFAIPMAVLAAR
                     NITTNKFLGGVFRMLLNLFRTIPALVFAALFTSIFGFGSFSGMLALFVFTLGLVAKLT
                     YEAIEGIDHGPLEALRASGAGHLAAFFYAVVPQVLPQYLSYVLYSFEINIRASAVLGY
                     VGAGGIAEYYDRTLSYLKYDRAGTIVLLTFVVILSIDLISARLREKLL"
     gene            7436..8233
                     /gene="phnE"
                     /locus_tag="VFD08_04515"
     CDS             7436..8233
                     /gene="phnE"
                     /locus_tag="VFD08_04515"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HZK10316.1"
                     /translation="MIDKYEMQKPKSKLFLYLIYALLFSLVFIWAFVGINYKGVMATA
                     RGTFRGMLNGILNPDLVYIKNTTIDGLGYALIETITIAISGTFISAMISLPFAFLASQ
                     NIVGKKISRLGKFFITCIRVFPELILAIIFIKVLGPGPMAGVMALGIHSIGMLGKLYS
                     EAIESMDMGSVEALEACGATGLQKLFHGVIPEILPALMGFSLYRFEINTRAASTLGIV
                     GAGGIGAPLLFAITGRNWSRTFTLVFSLIIMVILVDFFSGKIRKRIQ"
     gene            8233..8976
                     /gene="surE"
                     /locus_tag="VFD08_04520"
     CDS             8233..8976
                     /gene="surE"
                     /locus_tag="VFD08_04520"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZK10317.1"
                     /translation="MNILITNDDGIRAKGIKPLIDIAKKFGNVYVVAPKTEQSAISQG
                     ITIHEPLEIDKEEALFEGVVSSYSISGKPADCIKVALEYLRFDIDLVLSGVNNGPNLG
                     NDILYSGTVAAASEAAVFNIPAIAFSTDFGHFDMLETELPRTLEYILKEKLARSGVIV
                     NVNFPLNPHETSKGIKITHQGKRIFNAKFRHEEGKYWQEGSWGYIENGVDSDVTAVTE
                     GYTSITPLGIDRTFWKEFDLWTKKDHHKW"
     gene            complement(8991..9353)
                     /locus_tag="VFD08_04525"
     CDS             complement(8991..9353)
                     /locus_tag="VFD08_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZK10318.1"
                     /translation="MKLLINWILSAIVFMGLSEYTSLLVIDGFATALKLAVVISLIVF
                     LLRILSGFLKVMGCLTFGLSYLAGILISIFSMPIALMESMPYVRGYRIVSYKEAIIVS
                     IIMTVLSMLVFDTKKAPN"
     gene            complement(9350..10165)
                     /locus_tag="VFD08_04530"
     CDS             complement(9350..10165)
                     /locus_tag="VFD08_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZK10319.1"
                     /translation="MVIYDAFSDKVDKGNRTALVSAKGLSTKQMQEIAKKLGFAETLF
                     YEGLSLRYFSPEEEIDFCGHATLAFIWDRGQKEKRDREVLHTAVGDIPVFYSYKEDQL
                     EKVWMLQAPHRVKNISFDKNELCKALGIDLNDIDLNYPLKASYTGNWDIFVPIKNEAI
                     IDKMDPDMQALGALNRSMGVLSTHLYTMNKGNDYDIYTRDFAPACGIPEDPVTGSANG
                     ALFGLLRKEGFLTKQEEVVFAQGHALGYRGRVYAKEDGDSGRILVGGKAVVVK"
     gene            complement(10252..10437)
                     /locus_tag="VFD08_04535"
     CDS             complement(10252..10437)
                     /locus_tag="VFD08_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10320.1"
                     /translation="MVAKGRPKNEESYKKVQQEGFTFFVEKELAKELEGKYVELASYG
                     IIFPRYIVSLVKDMDEK"
     gene            complement(10671..10856)
                     /locus_tag="VFD08_04540"
     CDS             complement(10671..10856)
                     /locus_tag="VFD08_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10321.1"
                     /translation="MVETGSPVNEEGYKKIENKGFTFFVSNELARELDGHEVELSSIG
                     VLFPRYFISSVREVEEK"
     gene            complement(11073..12251)
                     /locus_tag="VFD08_04545"
     CDS             complement(11073..12251)
                     /locus_tag="VFD08_04545"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HZK10322.1"
                     /translation="MREVVIVSAARTPVGSFGGVFKNTSAVELGKVAAKEAIKRAGIT
                     PEQIEDVYIGNVLPAGQGQNVARQVSLGVGIPIEVPAMSINIVCGSGLRSVSLASQLI
                     SNGDADIILVGGTENMTMAPYYAHTARYGARMGDTKLVDGMIYDGLWDIFNNYHMGIT
                     AENIAEQWNLSREEQDQLAVTSQNRAEAAQKEGRFKDEIVPVPVPQRRGDPVIVEDDE
                     YIRKGATIEGMAKLRPAFKRDGTVTAGNASGINDGAAMLVIMSKEKADELGVKPLATI
                     KAHATKGVDPSIMGTGPIPATKAALENAGLKIEDIDLIEANEAFASQALSVAKELNFD
                     MDKVNVNGGAIALGHPIGASGARILVTLLYEMARRDAKKGLATLCIGGGMGTTLIVER
                     "
     gene            complement(12710..14779)
                     /locus_tag="VFD08_04550"
     CDS             complement(12710..14779)
                     /locus_tag="VFD08_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZK10323.1"
                     /translation="MQLQELLAPKSVAIVGASNREGSLGFDTVRMIKNSGYRGEVYPI
                     NPKYKEILGLPVYPDLNSIGKSLDTVVLCIAARRVEDQVDIAIKAGAKSLVIFANCVL
                     EEDTTPNLEERIMSKCKEAGIPLMGHNSMGYYNNDIDLRICGFEAPDAGEKGNIAFIS
                     QSGSAFSTIGHNEPQLKFNLMVAAGTGHITSLSDYMLHALEMDTTKVLGLYMESVRKP
                     DKFIEALELAAEKRIPVVAMKVGKSELGAKFALSHTGGLAGDDDAIQAVFNHYGVHRA
                     QSLDELGASLALFSFWPNDMHQGKTVAIADSGGERNLLADAAEDVGLEYAALSEETME
                     KLDEIQEYGQDAANPLDPWGTGIDFERIFAESMEIMLKDEAAKIGVISQDLRDGYYLS
                     EGCVGALAYGHEKTGKPVAFVTNFGGVRRKELTKKINNIGIPVLVGTEPSLRAIKNFE
                     NQASFLYEKSNEIKEEIPEKHLSYLENLDVLKEAYALEVLRDLGLPMTESHLVTSEQE
                     IKKLDLHYPLVLKTAVDGILHKADVKGVKVNIKTETELLDAYRDMEKRLGKECLIQPM
                     STFDVELILGMKDDPIFGPLMIIGAGGIFTELLRDRFIVLPKASRKEIEEGLNNLQIH
                     KLFEGFRGSEPINKEVLIDVILRFGEVIQILSPYIKEMDLNPLVIKGDKITGLDALIV
                     TKSGENM"
     gene            complement(14944..>15688)
                     /locus_tag="VFD08_04555"
     CDS             complement(14944..>15688)
                     /locus_tag="VFD08_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZK10324.1"
                     /translation="FLTFLYTMAISMNLINPAIEPTEKVMIWAAFNVMPKLVGVLVLT
                     GIMAAGLSSASTFLSVSGFSATNDVFKFKFRDDKHQLWSSRWIMLGISIIALILAYFN
                     PPAIRILSWFASTIIAASWGPVAFASIWSDKITEKGAFWGMLMGFLGQIGAESLTRFT
                     DMSLTGFFHPFFIGVYLNLIAIYLGSKSSTVTEVEKTYLARMRVIPEAELFAKDYKID
                     KLYAKILMSAGVLIAVLLVVLWAVPVMNA"
BASE COUNT         4580 a         3335 c         2733 g         5040 t
ORIGIN      
        1 gttgaacccg cacatccaaa aggatactac cccctcaagg tagcgcgtct gccgattccg
       61 ccactttcgc gagtgcaaac aaattatacg aaacacagca ggctttgtca agctgtaaat
      121 tcacctctta cccagatgta agggcctatt tgctggtaaa ttatagcaag ttctcctctt
      181 gtaaaaaagt tcttaccttc tcatacaatt cttctttgct cttgttattt tcgataatat
      241 aacttttttt tagtttttct tcccgaggat aatcaaaggc agacattttt tttaaagctt
      301 cctctttgct aatcccgtct cttttaatca aacgatccaa ctgggttttt ttatccacag
      361 caagataaag tactttatta agatatttat ccatacccga ttcaaaaagt agaggggcat
      421 catgaaaggc aatatgacct tggcctcttt taaacatgat tttgataatt aagggatgca
      481 gaagcttatc taattctttt cttctttttt tatctttata tataatggag gctagtattt
      541 ctctttgtag cttttcttcc ttaaaggcct cagggaaggc cttcttgaca agaaaatagc
      601 cttccctacc aggttccata acctcccgag caataagatc agcatctatc accataaagc
      661 caagctcttt taaataagaa cttagggttg atttgccact ggcaatatcg ccggtaatac
      721 cgtaattaaa tttcatacca gctctcccct acatttatac cagttttcaa cgggacactc
      781 agttcaacaa cattattcat cgtttcttca atcagcaatt taacctcatc cagctcttgt
      841 ttttttacat ctaaaattaa gctcatcatg gacttgaaga ataattttac tttccaactt
      901 cctttctttt aactttctcc aaacttgaat cattgctatc tttataatat ctgctgcact
      961 tccttgaatc ggtgtattca ttgctgtgcg ctgggcaaaa cttcgcatat ggaagttttt
     1021 ggagtgaatt tgaggaattt cccgtatacg tcctagaaga gttttaacat agccatgctc
     1081 tttggcaaag tctacttggg aatcaagata agccttgata cttgggtagt tttcaaagta
     1141 cttctcaata tagtcttttg ctgctgcaat gggaatatca atgttttctg ataagccgaa
     1201 atcgcttata ccgtaaacga taccaaaatt aactgcctta gcctccctgc gctgatggct
     1261 agtcacttct tctaaggggg tgtcaaaaac cacagaagct gttagtttat gaatatctaa
     1321 atcttttttg taaccctcca gcataactgg atcttcagaa aaatgagcta aaactcgaag
     1381 ttcaatctgg gaatagtcag cactaagaag taaataattc ttgtctcttg gaacaaatat
     1441 ttttcttatt cttcttcctt cttcgtatcg tatagggata ttttggagat ttggctctgt
     1501 tgaactaagc cttcctgtta ctgtaactgt ttgatttaaa gaggtgtgta ttttaccgtc
     1561 ttgatcaatc acttttctaa gcccatcaat gtaggttccc ttgattttgg ctagggctct
     1621 atagtgcaat atatcttcaa taatcggatg gcggccttgt agcttatcca aaacttcttt
     1681 gttggtagag tatcctgtct ttgttctttt gattacagga agaccaagtt cttcaaaaag
     1741 taccactccc agctgtttag gcgaccttac attaaagggc tcttccacat aagaaaaaat
     1801 tcgctcttct acctctttta atcttccact aataagttca tcttctttat caagttcttc
     1861 tagatcaaca ttaaaacctt ctttttcaat ggaggcaaga acctctacca gagggatttc
     1921 tacctcattg tataaagaca gcattccttt tgttttaagt gcctccagct gctgtatgtt
     1981 tagtttttca atggtatcaa gcatcttggc cataaaaaag tcatccattt cgcttggttc
     2041 ttgcaaacca gacaaatcaa ttgctatatt gcttttaaga tctggatgga gaagatagtg
     2101 actaatcata atgtcatctt ttttatatgg tagagggatt tccttaagat aaagatcctt
     2161 aagattatgg ctaatcagat tcttggctcc agaaaagtct atatcaccgg taaatacata
     2221 gtatttacct ttttctttta ctccatgaaa gtccttataa ttccagtaga ctacagaacc
     2281 ctctaggggg ggccttgtat gaaggatttc tacatcctct tcatagttca cttcttcttc
     2341 tacttcaata ttctttaaaa aacttctaag ttcatatttt tctaaaattt ccctggcgct
     2401 ttgataattc attccactat agctaaagaa agcttcctcg aattccactg gagcatccaa
     2461 gtcgattttg gcgaggtctc tacttaggta agctaattct cttccttctt caagaagttt
     2521 ttttattcgt ggagttactt ttccgggatc cttgtagaca tcttcaagat gccttcctcc
     2581 ctctagtagc tttagggccg ttttcgcccc tactcccttt accccaggaa tattatctga
     2641 ggggtctcca cgtagaccca tttcatcgat tacttgattg ggataaagcc caagctcctc
     2701 gactacttcc ttctcatcgt agcttataag cgtgctaatt ccctttttgg gaatggcaac
     2761 ggtaacgctc tcatttacta gctgaagggt atccttgtcc cctgttaaaa taacagaatg
     2821 aaagccctct tcactcgcct tagcagaaag ggtacccatt agatcatctg cttcataacc
     2881 aggaagttca atgcgtgcta gccctagagc gtcaaggatt tcttttagta caggcatttg
     2941 catagcaagc tcatcaggca ttcccttgcg attggcttta tagccatcat atttaagatg
     3001 gcgaaatgtt ttttcttttg tatcaaaagt aataatcagg tggctaggat tttctctttc
     3061 caacatattg tctagcatac gtaaaaaacc gtgaattccc ccagtaaaaa acccttcttc
     3121 tgtgcgaagg gggggcaagg cataatatgc ccggttcaca agagaattac catcaacgat
     3181 tagtagtttg ttcataggtc catcctttca tctttatact tttcaagaaa ggttttcttc
     3241 tttcccttat ctactgtacc caataccgaa cccaaatgaa catgtttcat gctggcaaat
     3301 attgactctt ccactcgggg aaaatcttct ttcaatattg gtagtaattt ttttatcttt
     3361 tgcctggaag attgattttg tttttctgcg acaggacaag cacaatctaa aaaactaagt
     3421 ccagcttcct cctgccatcc aagtatatct ttttccctta gataatatag gggccggatt
     3481 aattccatcc cagggaagtt ttgagcatgg agcttgggca tcatagccat tgtaatccca
     3541 ccttgaagta gattaagtag gattgtttca attacatcat catagtgatg acccaaggca
     3601 agtttattgc atccttcttt tttagccaag tcataaagag cgcccctgcg cattcttgca
     3661 caaagaaagc aaggtttttc actaagcttt aaagatactt cgaaaacatt tcttgaataa
     3721 atttttatgg gtatatttaa ataatcagcc attttttcca aatgatctct atgctgggga
     3781 gcatagcctg gatccattgc aataaagcta agctcaaaat tcctattacc atgacgataa
     3841 agttcctgaa aaagcttggc taagagaaaa gagtctttcc ccccgcttat ggccactgcc
     3901 acctggtctc cttcttctat cagttgatat tcttttatgg ctcggataaa tggagtccat
     3961 agcttatgtc gatatttttt ttgtatactt tgttcaatcc attcgcaact tttcatttcc
     4021 acctctaata tgtagtataa tcaattcatc gaggaggtac aaggtgaatc cttttatccg
     4081 tacacaagct ctcctagaag gagactttaa aaaaattgag aatgccagtg ttgctattat
     4141 tggtctaggc ggagtgggaa gccatgccgc tatggcccta tcacgcatgg gaataagaaa
     4201 acttattctt atggatcctg ataggattaa aataagtaat ttcaatcgtc acgcccaggg
     4261 ctacactagg tatttagatc acaataaggc agaggccatg aaagaagaaa tcctttcttt
     4321 tcgccccatg gatatccttg ttattccaaa attatatgac ttgtcagaag aagacaatct
     4381 attttcacaa gattttgata ttcttattga ctgcatcgat gtgatgacct ttaaaattcg
     4441 cctgatcgag gcctgccagc agcgtaaaat cccctttatt tcctctctag ggacaggaaa
     4501 ccgtcttcag ccaacagaac ttgaaataac atcgatttat aagacccaag gctgtcccct
     4561 agcgcgggtg ttgcgaaagg aagcccggaa aagacagata gagaattttc ctgtaattat
     4621 ttcaaaagaa aaagccagaa atatcgtact taaggaaaag gagggaagcc ggcatttacc
     4681 ggcctcaagt tatttcactc ccgcagcagc aggacaactc cttgcttact gggccataaa
     4741 aagcctattg gataaaacct aagctatatt tttgcatctt tacaagaata tcgcctctgt
     4801 tataatagtg ttataaatac tctaaaatac accaaggagg tagaatgaaa aagtttttgt
     4861 caattgccat gattgcacta ctggtattca ccctagctgc ctgtacagat gaggcgagtg
     4921 aaactgagag tgcacaagaa gagaccgcag aaacacaaga ggaagaggag gaagtagtag
     4981 aagaagccct agttattgaa actctaaaag ttcaaatcac tccctcaaaa gatgcagcac
     5041 gtctagatgc ccagcgtaaa ccactacagg atatgcttga agaaaaactt gctatgccag
     5101 tagaggtttc cattggtacc gactatagtg ctactatcga agcaatgaga agtggtcaaa
     5161 tccatgtagg ttttcttgct ccaacccaat atgttctagc ccatggtcaa gatgcagctg
     5221 atgtaatttt aaaatctctt cgctatgatg ttgacgataa tggagataag cttgaggatg
     5281 cccctctagt agataatttc cgttctcaac ttgtagctgc tacagatagc cctattaact
     5341 ctgtacaaga cctaaaagga aaaacgattg cggttgcaag ttttacttct accagtggtt
     5401 ttgtatggcc agccaacctc cttgctgata gcggacttga tccccaagcc gatgttgaat
     5461 gggtcaatgt tggtggacac gatttagcca ttcaagctgt ttacaatgga gaaacagatg
     5521 cagcctttac ctttaaggat gcccgttttg ttcttaaaga agaacaccct gatgtttatg
     5581 ataaagtacg ctttgttctt gatacagaag gtatccctaa tgatacaatt tctgttattc
     5641 cacaaatgga ccaagaactc cgagatcgta ttgccgatgc ttttttaagt attgttgacg
     5701 aggaagaagg tcttgaagtt ttccgcttga tctacgagtg ggaaggcttt gcaaaagccc
     5761 aagatagtga ttacgatatt gtgcgcacct atctacaaag acaagaagaa tgggactttt
     5821 aaattaaaaa gctgtcgaaa gacagctttt tccttaagga gatattatgc cacaacttga
     5881 aataaagaat atatcaaaag tttatcccaa cggagttcgt gcccttgatg gcgttagttt
     5941 tcaagtaaat aaaggtgagt ttgtatccat tattggtctt tctggtgcag gcaaatctac
     6001 cttgctgcga agcataaaca aactcattga aatgtcgggg ggagaaatta tttttgaagg
     6061 aaaagatatt gcttcagcca agggaaagga tttacgcctg attagaagag atatgggcat
     6121 gatatttcaa agctttaatc tggtaaaaag aagcaaggtt ataaccaatg tattagccgg
     6181 acgaattgcc tatcataagc cttggagaac aattttgggt ctctttccaa aagaagataa
     6241 ggccattgcc tttgaagccc ttaatcgcgt aaatatccta gaaaaagcct atagcagggc
     6301 cgatcagcta agtggaggtc agatgcaaag ggttgccatc gcaagaagtc ttgctcagga
     6361 accaaagttg cttcttgctg atgaaccagt ggcaagccta gatcctctta ccaccattca
     6421 ggtaatggat gatctaaaaa gaatcaacag agaactgggt attaccacgc ttgtcaatct
     6481 tcaccatgta gatcttgcct taaaatactc cactagagtt atcggcctaa gagaaggtaa
     6541 acttgttttt gatgggccag ctaacaacgt agatgatgaa atcttaaagc aaatttatgg
     6601 gcgcgagctc ttaagtaatg aagtttttgg agaagacaat gaaaaatgat gataaattag
     6661 ccccttctct tccaaaaaaa cctgctgaac ttaagcggaa tttatctatt cttctttttc
     6721 tttctattat ttatgcttcg ggaagtttta ccaactttag cctgtcggag ctcctaggaa
     6781 atttaggtga agctggcgat ttactcggtc gaatgttttt gcctcccgac ttttcctatg
     6841 caagaagact atttttgcca atggtggaaa ctcttcaaat ttcttttctt ggaacggtaa
     6901 ttggctctat ctttgctatt cctatggctg tacttgcagc gcgaaatatt actactaata
     6961 agtttcttgg aggggttttt cgcatgttat tgaacctctt tcgtaccata ccagctcttg
     7021 tttttgccgc gctttttact tccatctttg gttttggtag tttttctggc atgttggcgc
     7081 tttttgtctt tactctaggt cttgttgcaa aacttaccta tgaagccatt gagggcatcg
     7141 atcatggccc cttggaagcc ttaagagcct ctggagcagg gcatctagca gctttttttt
     7201 atgctgtagt ccctcaggtt ttaccacaat atttaagcta tgttttatac tcttttgaga
     7261 tcaatattag agcatcagcc gttctaggtt atgttggtgc tgggggtatt gcagaatatt
     7321 acgatcgcac cttaagttat ctcaaatatg atagagctgg aaccattgta ctgctaacat
     7381 ttgttgtgat tttgtctatt gatttgattt ctgcccgatt aagggagaaa ctcctatgat
     7441 agataaatat gaaatgcaaa aaccaaaatc aaaattgttt ctttacctta tttatgccct
     7501 tttattttcc cttgtcttta tttgggcttt tgtagggatc aactacaaag gagttatggc
     7561 tacagcccgg ggcacctttc ggggcatgct aaatggtata cttaatccag atctagtcta
     7621 tatcaagaat acaaccatag acggactagg ctatgccttg attgaaacca taaccatcgc
     7681 tataagtggg acatttattt cagctatgat ttctttaccc tttgcctttt tggcttcgca
     7741 aaatattgtt ggaaaaaaaa taagtagatt gggtaaattc tttattacat gcattcgagt
     7801 gtttcccgaa ctaatattag ccattatttt tatcaaggtc cttggacctg gtcccatggc
     7861 tggtgttatg gctcttggaa tccactctat tggaatgcta ggcaagctct atagcgaagc
     7921 cattgagagt atggatatgg gctctgtaga ggctttagaa gcctgtggag caacaggtct
     7981 ccaaaagctt tttcatggtg ttataccaga aattttacct gccctaatgg gctttagtct
     8041 ctatcgcttt gagatcaata caagagcagc ctcaacccta ggtattgttg gtgctggtgg
     8101 tattggagct cccctactat ttgctattac tggaagaaac tggtcgcgaa cttttaccct
     8161 tgttttctca ctaattatca tggttatact cgttgatttt tttagtggaa aaattagaaa
     8221 gaggatacaa taatgaatat acttattaca aatgatgatg ggattagggc aaaaggcatt
     8281 aagcccctta tagacattgc caagaaattt ggcaatgtct atgttgtcgc tcccaaaaca
     8341 gaacaatctg ctatttccca aggaatcacc attcatgagc ccctggaaat cgacaaggaa
     8401 gaagctttat tcgaaggtgt tgtctcatct tactccatta gtggaaaacc agctgattgt
     8461 ataaaagtgg ccctagaata cctgcgcttt gatatcgacc ttgtgctctc aggagtcaat
     8521 aatggcccaa atctaggcaa tgatatactt tactcaggca cagtagccgc ggcaagtgag
     8581 gctgcagtct ttaatattcc agccattgct ttctcaacgg attttggcca ttttgatatg
     8641 ttagaaaccg aactcccccg taccttagaa tatattctaa aagagaaatt agcccgcagc
     8701 ggagtgattg ttaatgtcaa tttccccctt aatccccatg aaacttccaa gggaataaaa
     8761 attacccatc agggcaaacg tatttttaat gccaaattta gacatgaaga aggaaaatac
     8821 tggcaggagg gcagctgggg ttatattgaa aacggtgtag acagtgatgt tacagctgtt
     8881 accgaaggtt acacctccat aacacccctg ggaattgata gaactttttg gaaagaattt
     8941 gatctttgga caaaaaaaga ccaccataaa tggtagtccc taaaagcttc ctaattggga
     9001 gctttttttg tgtcaaaaac aagcatactt aaaactgtca taataatact aacaataatc
     9061 gcttctttat aactgacaat cctataccct ctaacatagg gcatcgattc cataagggca
     9121 atcggcatag aaaaaataga aattaaaata cctgccaaat aactaagtcc aaaggttaag
     9181 caacccatca cttttagaaa gccactcaga attctgagta aaaatactat taaactaata
     9241 acaacagcca gttttaacgc ggtagcaaaa ccatcgataa caagaaggga tgtatactct
     9301 gaaagtccca taaatacaat cgccgaaaga atccagttta tcagtaattt cacttaacaa
     9361 ccaccgcctt tcctccgaca aggattcgtc cagaatcccc atcttccttt gcataaactc
     9421 gtcccctata accaagtgca tgcccctggg caaaaaccac ttcttcttgc ttagtcaaaa
     9481 aaccctcttt tctaagaaga ccaaaaagag caccattagc agagcctgta acaggatctt
     9541 ctggaatccc acatgctggg gcaaaatccc tggtatatat atcataatca ttgcccttat
     9601 tcatggtata aagatgggtg cttagtaccc ccatagaacg atttaatgcc cctagagcct
     9661 gcatatcagg atccatctta tcaataatag cctcattttt tataggtaca aatatatccc
     9721 aatttcccgt ataggaagct tttagaggat aatttaagtc aatatcattc aaatcaatgc
     9781 ccagcgcctt gcagagctcg tttttatcaa aggatatatt tttaacccta tgaggagctt
     9841 ggagcatcca gactttttct agttgatctt ctttataaga ataaaaaacg ggaatatctc
     9901 ccactgctgt atgcaaaact tccctgtctc ttttttcttt ttgaccacga tcccatataa
     9961 aggccagggt ggcatgacca caaaaatcga tttcttcttc tggagagaaa tagcgcaaac
    10021 taagtccctc atagaaaagg gtttctgcaa aacctagctt tttagcaatt tcttgcattt
    10081 gtttagtgct aagtccttta gcactaacaa gagctgttct atttccttta tccactttat
    10141 cggaaaaggc atcataaata accatatttt taattctcct tactgaataa aaaagtagct
    10201 aaattaccag ctatatatat atatatatta tgtatgggga gataaatctc cctatttttc
    10261 gtccatgtcc ttaaccagac tcacaatata gcgtggaaag attataccat aactagcaag
    10321 ttccacatat ttaccctcta gttctttggc aagttctttt tcaacaaaaa atgtaaagcc
    10381 ttcttgctgg acttttttat agctttcttc attttttggc cttccttttg caaccaccgg
    10441 ttgctctctt gggccagctc aaccgccgac atgcactgtt ttaatggtaa attggctgag
    10501 tccttttttc tctaacttag gaatagactt tttattaaat gaaatattca ttttcaccct
    10561 cccttaaatt atatatacca agcatagcct tttcaaaaca tatcattaac tcaaataaaa
    10621 aagtagccga taagcagcta cgaattatgt ataggaggat cttaatcccc ctatttttcc
    10681 tcgacttcgc gaacggatga tataaagtaa cgtggaaata ggacgccaat gctacttagt
    10741 tccacctcat gaccatctag ttctcgggcc aattcattag acacaaaaaa agtaaaacct
    10801 ttgttttcga tttttttgta gccttcttca tttactgggc ttcctgtttc aaccaccggt
    10861 tgacgactag gaccacctca gccattttgt acaacaattt tcatggtaaa ttcagtaaga
    10921 cctttttctt ctaatttggg aattaatttc tctctaaatg ctatattcat ttactcccct
    10981 ccttcttaaa tcatatatac caatcataag actttcaaaa caccttattt attaccttta
    11041 aaaaagcccc ttctttcgaa gaggctttaa gcttaacgtt caacaataag cgtagttccc
    11101 atacccccac caatacatag agtggcaaga ccttttttag catctcttcg cgccatctca
    11161 taaagtagag ttacaaggat tctagctcca cttgctccaa ttgggtgacc aagggcgata
    11221 gctccgccat tgacattcac cttatccata tcaaaattaa gttcttttgc tacactaaga
    11281 gcttgcgatg caaaagcttc atttgcttca attaaatcta tatcttctat cttaagacct
    11341 gcattttcta atgcagcttt tgtcgctgga attggacctg tacccataat cgaaggatct
    11401 acacccttag tggcatgggc cttgattgtg gctaaaggct taactccaag ttcatctgct
    11461 ttttccttgc tcataataac cagcatggcc gctccatcat taattccaga agcattacct
    11521 gctgtaactg taccatctct tttgaaagct ggtcttagtt tagccatacc ttcaatagta
    11581 gcacctttac ggatatattc atcatcttca actataacag ggtcgcctct tctttgagga
    11641 actggaacag gaactatttc atctttaaaa cgtccctctt tttgagcagc ttccgctcta
    11701 ttttgtgatg taactgctaa ttgatcttgc tcttctcttg ataaattcca ctgctcagcg
    11761 atattttctg ctgtaatacc catatggtaa ttgttgaaaa tatcccaaag tccgtcataa
    11821 atcatcccat caaccaactt tgtatcaccc atacgagcac catagcgtgc tgtgtgagca
    11881 tagtaagggg ccatggtcat attttctgtt ccaccaacta gaataatatc tgcatcgcca
    11941 ttactaataa gttggcttgc taaacttact gatctaagtc cagatccaca tacaatgtta
    12001 attgacattg ctggaacttc tattggaata cctactccta agcttacttg acgtgcaacg
    12061 ttttgtcctt gaccagcagg caaaacattt ccaatataga catcttcaat ttgttctggt
    12121 gtgattcctg ctcttttaat ggcttctttt gcagcaacct tacccagttc tactgctgaa
    12181 gtatttttaa atactcctcc aaagcttcca actggcgtac gagctgctga tacaattact
    12241 acttctctca ttctaatctc ctttcgatac gtagattctc atcttaaagt atagtcctgg
    12301 ccttgttaaa agtcaagatt agcccaaaag taggcttaat gtaatacact tgcctttatt
    12361 tagcaggtgc tattacatac atacccaatt aaataaaaaa actactctat tattcaatat
    12421 tgttgagttt tgccaatgta ttttgctttt ttagcttatt tgacaggctt ataaagctcc
    12481 tgctcttaga taaagggtct atatgacaag ttttgctctc ttattttcat tgacatatga
    12541 acacaaagga ttatattcac attaaaatag tagctggtca ctagggaaac tctaagaaat
    12601 tcaatagcga ctttattgtt atatgtaggg ggtgttcatt acactctctt tgaaaagcaa
    12661 aagcaagcta aattactagc ttgcttttac taagccttgt ttattcaaac tacatatttt
    12721 ctccagattt agtgacaatt agcgcatcta atcctgttat cttatctcct tttattacaa
    12781 ggggattaag gtccatctct tttatatagg gagacaatat ttgaattact tccccaaagc
    12841 gaagaatcac atcaattaac acctctttgt taattggctc agaaccccta aagccctcaa
    12901 acaacttatg aatttgaaga ttatttaagc cctcttcaat ttcttttctt gaagccttgg
    12961 gcaaaacaat aaaccgatca cgtagtagtt ctgtaaaaat accaccagca ccaataatca
    13021 ttaaaggacc aaatatagga tcgtctttca ttcctagaat cagttctaca tcaaaagtgc
    13081 tcataggttg gattaaacac tctttgccca gtcttttttc catatctctg taggcatcta
    13141 aaagttctgt ttcggtcttt atattaacct ttacaccctt aacatcggct ttgtggagta
    13201 ttccatctac agcagttttt agaacaagtg gatagtgcaa gtctagcttt ttaatctctt
    13261 gctcgctggt taccaaatga ctttctgtca taggtagtcc caagtctctt aagacctcta
    13321 aagcataggc ttcctttaac acatcaagat tctctaagta agacaggtgt ttttctggaa
    13381 tttcttcttt tatttcattt gatttttcat aaagaaaaga agcttgattt tcaaagtttt
    13441 taattgctcg caaggagggt tctgttccca caagaactgg gatcccgata ttattgatct
    13501 ttttagtcag ttcttttcgt cttactccac caaaatttgt tacaaaagca acgggcttgc
    13561 ccgttttttc atggccatag gctagagcac caacacatcc ctcactaaga tagtatccat
    13621 cgcgaaggtc ttgggaaatg accccaattt ttgctgcttc atcctttaac ataatttcca
    13681 tcgattcagc aaaaattcgc tcaaaatcga ttcctgtccc ccaaggatct agtggattag
    13741 ctgcatcttg accatattct tgaatttcat caagtttttc catggtctcc tcagaaagag
    13801 ctgcatattc taaaccaaca tcttctgccg catcagccag aagattgcgt tctccaccag
    13861 agtcagctat tgcaaccgtt tttccttgat gcatatcatt tggccaaaaa ctaaagagtg
    13921 ctaagctggc acctaattca tccagggact gcgccctatg gactccataa tgattaaaaa
    13981 ctgcctgaat tgcgtcatca tcaccagcaa gtcctcccgt atgagaaagc gcgaattttg
    14041 ctcctagttc tgactttcca accttcatag caacaacagg gattcttttt tcagcagcaa
    14101 gttccagtgc ctcaataaac ttatcaggct ttcgaactga ttccatataa aggcctagta
    14161 cttttgttgt atccatttct agggcgtgaa gcatataatc tgacaaactt gtgatatgac
    14221 ccgttccggc tgcaaccatt agattaaatt ttaattgagg ttcattatgt cctatggtgc
    14281 taaaggccga acctgactga gatataaagg caatatttcc tttttctcct gcatccggtg
    14341 cctcgaaacc acatattctt agatcaatat cattgttata atagcccatt gagttatggc
    14401 ccataagcgg tatacccgcc tctttgcatt tggacataat tctttcttct agattcggtg
    14461 tcgtatcctc ttctaatacg cagtttgcaa atataaccaa tgatttagcg cccgccttta
    14521 tggcaatatc aacttgatct tcgactcttc ttgcagctat acatagcacc acagtatcaa
    14581 gggattttcc aatagaattt aaatctggat atactggaag tccaagaatt tccttatatt
    14641 ttgggtttat tggatagacc tctcccctat atccgctgtt ttttatcatt cttactgtat
    14701 caaatccaag acttccttct ctatttgatg caccaacaat agcaacactc tttggagcca
    14761 gtagttcttg taattgcatc ttcccctcct ttataaattt taatctggca ttttgcccaa
    14821 gtaaagtcca ttaatacaat agctccataa ttgaccaaaa tatcaattat ggagctagcc
    14881 tttgtcccga ggcaaacttc acactgcccc atattgacct tttaattaat ctatactaat
    14941 taattatgca ttcataactg gaactgccca gagaacaact aaaagtacag caattaaaac
    15001 acccgcactc attaagattt ttgcataaag cttatcaatc ttatagtctt ttgcaaagag
    15061 ttcagcctct ggaattacac gcattcttgc taagtatgtt ttttctactt cagtaactgt
    15121 tgaggatttg ctacctagat aaatagcaat taggtttaaa tacacaccaa taaagaaggg
    15181 gtggaaaaaa cctgtaagac tcatgtcggt aaaccttgtt agagattctg ctccgatttg
    15241 gccaagaaat cccatcaaca taccccagaa ggcacccttt tccgtgattt tatcactcca
    15301 tattgatgca aaggcaacag ggccccatga agcagcaata atagtacttg caaaccagga
    15361 aagaatccga atggcaggag gattaaaata agctaaaatt aaggctataa tagaaattcc
    15421 tagcataatc catctacttg accatagttg gtgtttgtca tctctaaatt tgaatttaaa
    15481 cacgtcattt gtggcactaa atccacttac tgataggaaa gtggaagcag aggataatcc
    15541 cgctgccata atacctgtaa gtactaaaac acccactaat ttaggcatta cattgaaggc
    15601 tgcccaaatc attacctttt ccgttggctc tattgctgga ttaataagat tcattgaaat
    15661 agccattgta taaaggaatg ttaagaac
//
LOCUS       DASRNA010000166         3565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1018059,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000166 DASRNA010000000
VERSION     DASRNA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018059"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..876)
                     /locus_tag="VFD08_04560"
     CDS             complement(379..876)
                     /locus_tag="VFD08_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HZK10325.1"
                     /translation="MPNKKKKQDSLMDKDDMEIVCDLDLNKYLGKWFEIAKFSARGQK
                     GLNNVTALYTLKKSGKIAVYNSGYKKNKKIGIRGSAWLRNKNCSGALYVRFFWPSKAE
                     YNVIKLAPDYRYALVMGDSKSKLWILSRTAKMNKKDYYEILKFLDENNFNTEKLMKTI
                     QNKQV"
     gene            1181..1645
                     /locus_tag="VFD08_04565"
     CDS             1181..1645
                     /locus_tag="VFD08_04565"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZK10326.1"
                     /translation="MTVKDDKYYIRKAIELAYEAKEDGDNPFGSILVDSKGNILMEDK
                     NTQITKNDITGHPELKIAQRAAAKYDEGFLKECTMYNSAEPCTMCTGAIFFSGIGRIV
                     FGISKERMRELKNKGGPGIKYSIHELLGTSGKDIEVVGPMEDMEEEVEGPHL"
     gene            complement(1949..3160)
                     /locus_tag="VFD08_04570"
     CDS             complement(1949..3160)
                     /locus_tag="VFD08_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZK10327.1"
                     /translation="MKTIYFEKDIPRVLATKFSSRYAKKLLYTGLNAVKYEKDLAEPP
                     LPGDWVRVRNIYCGVCGTDVSFFKATTGTSSAFEPIPSSKRTYLGHENIGKVIEIGSE
                     VKDLKVGDRVTIRAYMSGCDTKGIKDRCHYCKEGNYNFCLNYGAPSPHKNLGDTGAGF
                     SDRFIYPEKGLAKVYDEISDLDATMIEPAAVSIHSVLTSPPQKDEKILVMGCGTIGLG
                     VVQAIKILEPDCEIWIMERVKMKQDFAKKLGADYVLKGDPYTASSKATGGSQVYTGMN
                     KNKYFFGGFDRIYDCIGGNWSNNTGLRLLKARGTFVKIGHHMQAITFDESPIWWQELK
                     IIGVDAHGMEKWEGRKLYTFDLAQEWIRDGIYSVDGFITHHFDLDDYKEAMRLAMNNP
                     PDVIKIVLDCQ"
BASE COUNT         1084 a          684 c          673 g         1124 t
ORIGIN      
        1 agtgaagtgg aatctcacta tcttttatta tttttatgaa gagctggtac actttctgat
       61 tgacaaaagc attcatatct tagttacgcc ttgggccagt aaagtccata tatttactgt
      121 ctaaggtgtt attacttcca taaagagaaa gtttgaatga ttttttatgc ttaggtttga
      181 tcgatgggtt acttttgtgt ttgtctctta cattaatatt ctctattaag ctcttatata
      241 tcttactacc atatcattgg gtccggaatt accctcagaa ttaacttcaa aactgggggt
      301 aattccaatt ggaactaggt gggttggttt gttttacagc tttttggagc tttcaaaggc
      361 gataccttat ttattaatct aaacctgttt gttttgaatg gttttcatta gtttttcggt
      421 attaaaatta ttctcatcta aaaactttag gatctcatag tagtctttct tattcatttt
      481 tgcagtccta cttaaaatcc aaagcttact tttactatct cccattacta gagcatatct
      541 atagtcgggt gctagtttga ttacattgta ttcagcttta gagggccaga aaaaccgaac
      601 atataaagct ccactacaat ttttattcct tagccaggca ctgcccctaa ttcctatttt
      661 cttgtttttc ttgtagcccg aattgtaaac agcaattttc ccgctttttt taagagtgta
      721 tagagcagtt acattattaa gacccttttg tcctcttgct gaaaacttag caatctcgaa
      781 ccatttaccc aaatatttgt ttagatctaa atcacaaact atttccatat catccttgtc
      841 cattagagaa tcttgcttct tctttttatt aggcatttta ttgcttcctt tccccacaat
      901 atgtctaaaa acttaaacct tatctataga ttacccctta accttagtaa tatttttcct
      961 tttatcttct tcatcaaaaa aatattaacc tagaccatta aatagaccaa aaaagaccaa
     1021 gtttagaaga aggagtattt tatttacttg tgagtaatag gggtaagaaa atagtaaggg
     1081 cctagactca tactttaaaa accccatgca ccatcactac tttgaagctc acaatacaag
     1141 ggatgactaa attaccaaag ccttaaaaaa aggagagata atgactgtaa aagatgataa
     1201 gtattatata agaaaagcta tcgagctagc ctatgaggcc aaagaagatg gagacaaccc
     1261 ctttggatca attttggttg attccaaggg caatattttg atggaagaca aaaacacaca
     1321 aattactaaa aatgatatta caggccatcc agaactaaag attgctcaac gtgctgccgc
     1381 aaaatatgat gaaggctttt taaaggagtg caccatgtat aattctgctg agccctgtac
     1441 catgtgcacc ggcgccatct tttttagtgg catcggaaga atcgtctttg gaattagcaa
     1501 agaaagaatg agagaattaa aaaacaaagg aggtcctggc atcaaatact ctatccatga
     1561 gctattaggc actagtggta aagatattga agtggtcggt cccatggaag atatggaaga
     1621 agaagtagaa gggccccatc tataagaaag ataaaaagaa gggaagctct caagacaaaa
     1681 gcacctattt atttaggtgc tttcgtgttt tgctagagac aactagcgta gggacgaata
     1741 aatatagtgg tttttgtatc acccaaactc tttgtcccgg cgctttttac ctacccttaa
     1801 gtgaagtatg aaaagtccac tcataggcgg cttgggatgc tagtggtcct gtgtttttct
     1861 tgccaaaata ggggggttac taggctcatc cccgctgact aggaccacaa aaagtataat
     1921 tttaaaaatt gtatttgggc ctggattatt actgacaatc tagtacgatt tttatcacat
     1981 ccggtgggtt attcatagca agcctcatcg cttctttata gtcatctaga tcgaagtgat
     2041 gcgttataaa gccatcaaca ctgtagatcc catctcttat ccactcctgg gcaagatcaa
     2101 aggtataaag cttgcgaccc tcccactttt ccataccgtg ggcatccacg ccgataattt
     2161 ttagttcttg ccaccagatg ggagattcat caaaggtaat ggcttgcata tggtggccaa
     2221 ttttgacaaa ggttcctctt gcttttaaga gtctgagccc tgtgttgttg gaccaattgc
     2281 ctcctataca atcataaata cggtcaaaac cgccgaaaaa gtatttgttt ttattcatac
     2341 cagtataaac ttgactacca cctgtggcct tagaagaagc tgtataaggg tctcctttaa
     2401 gaacataatc agcacctagt tttttagcaa agtcctgctt catttttaca cgttccataa
     2461 tccaaatttc gcagtctggt tcaagaattt taatggcttg aaccactccc aagccaatgg
     2521 ttccacaacc cataacaagg attttttcat ccttttgggg aggagaggtt aggacagagt
     2581 ggatgcttac tgcagcgggt tcaatcatag tagcgtctag gtctgagatc tcatcataga
     2641 ccttggcaag ccctttttca ggataaataa agcggtcact aaaaccggcg ccggtatctc
     2701 ccaagttttt atggggagaa ggtgcaccat agtttaaaca gaaattatag tttccttctt
     2761 tacaatagtg gcaacggtct ttaattccct tggtatcgca gccactcata taggcgcgaa
     2821 tagtgacccg gtcaccaact tttaggtctt ttacttcaga accaatttca atgaccttgc
     2881 caatattttc atgacctaga taggttctct tggaactggg aatgggttca aaggctgaac
     2941 ttgttccagt ggttgcttta aagaagctaa catcggtacc acaaacaccg cagtaaatat
     3001 ttctaacccg aacccagtca ccaggtagag gcggttccgc tagatccttt tcgtacttaa
     3061 cggcattaag acctgtatag agaagttttt tagcatagcg actggaaaac ttggttgcaa
     3121 gaacacgcgg gatgtctttt tcaaagtaaa tggttttcat actactgtta acctccttaa
     3181 aaaatgttga atccttttaa cttgattata cactgaattt acaaagttgt aagaaggtta
     3241 ttgcaattgt gtaaatatgg taaaatgaaa taagaagagt taggaagcat tatcttcctg
     3301 tcatcctttt atgccatctt ctatttgttg gcagctagaa gtagataagt atgattatag
     3361 gcctacttta tcaatgcatc ctccataggg gaggtgagag cactggtcta tgtgcagaat
     3421 aaaaaagatc atgctctggg agcttagcta gcaaaaatgg cataaagagt aagaaaactc
     3481 tgtggatcaa agaaaaacta ggtgggttaa ggaagaaaaa gccccagcgg tagttagaca
     3541 tttagacctt tagctgtggc tacat
//
LOCUS       DASRNA010000167         5642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_864345,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000167 DASRNA010000000
VERSION     DASRNA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864345"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VFD08_04575"
     CDS             complement(<1..357)
                     /locus_tag="VFD08_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZK10328.1"
                     /translation="MYPLAAALLPHPPILISEIGGEEALEAKLTLEGIEEVARRFSSL
                     EPDTLVLISPHGNLFQDAICILDAPRLRGDLSKFGARDLQFEYETDQEMIKNLRKDTR
                     DFPLLFLDKKGARKLGT"
     gene            535..1677
                     /gene="iadA"
                     /locus_tag="VFD08_04580"
     CDS             535..1677
                     /gene="iadA"
                     /locus_tag="VFD08_04580"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HZK10329.1"
                     /translation="MILIKNIHVYAPEDLGIKDVFVSGEKITLIENEIPAFHKDIKVI
                     DGKGKILIPGLIDNHVHITGGGGEGSFKSRVPEITLSKLLKGGITTVVGLLGTDGSTR
                     NIENLVAKAKALKEEGLSAYVHTGSYAYPPISLTGSPGKDILFVEEVLGVKIALSDHR
                     SSSITNNELARLASEVRVAGMLSAKPGIVVVHMGEGKRGLEPILEILDSTDLPIKTFR
                     PTHVNRNPQLLKQALAFAKKGGNIDLTCGEKNELSTASVLRQAEKLMVPRENITLSSD
                     GYGSFSDYDQFGNLSHIGVASVSILFKELRDLVLVEGFDLERALIFFTSNVAKALHLY
                     PDKGCIKENSHADLLILDKDLALSSLIARGRLLLHKKELLVKGTYE"
     gene            complement(1724..2629)
                     /locus_tag="VFD08_04585"
     CDS             complement(1724..2629)
                     /locus_tag="VFD08_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HZK10330.1"
                     /translation="MIEIKKEVKEALDQGRAVVALESTIISHGMPYPENLETALQVER
                     IVRQAGAVPATMAILDGVMKVGLNKEELEYLATTKDALKVSRRDLPYVLASKKNGATT
                     VAATMIMAKLAGIKVFVTGGIGGVHRGGEDSFDISADLMELAKTSVAVVCAGAKSILD
                     IGRTLEVLETHGVPVLGYKTKEFPSFYTPKSGYFVDYTMESTQEAARLLKEKWDLGLE
                     GGAVLAVPIAEEAALDDGLIEGSIQTALLHAKELGISGKEVTPYLLDELKRVTKGDSL
                     KANIELVYNNARVGSALAVDLAKLG"
     gene            complement(2634..3608)
                     /locus_tag="VFD08_04590"
     CDS             complement(2634..3608)
                     /locus_tag="VFD08_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZK10331.1"
                     /translation="MKKEEAKIGKIKTDPKITLIGACNLDLQGISSQDLKLYDSNPGK
                     LTTSAGGVARNIAENIARLGLSTRLLTTLGKDKEGDFIRSSTEKLGVDMQDSLYLENT
                     PSSVYLSILDKKGELVVAINQMGILEKLNKEYLQEKKELIKESPMIVLDANLQEEVID
                     YLCKNHQDQRIFVDPVSTVKSLKLKNSLKYLYAIKPNIYEMQSLLGRKLSGFEDYKKA
                     LYQVLELGVKEVYLTLGQEGVLVADKNQIIHASAQVENVKSVTGAGDAFLAAAVYANY
                     HGWDLEHLASFSQALALIVLGQQRDRVYFEEEKIRQRRKDVKTKSLLW"
     gene            complement(3732..4325)
                     /locus_tag="VFD08_04595"
     CDS             complement(3732..4325)
                     /locus_tag="VFD08_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HZK10332.1"
                     /translation="MCVHYFAPRAEMLQERDKGKISSKKPYPYRDIFPGIRAPIYLAA
                     KEGLELVELLWGYPVSWQKGPVFNTRLESAIKGKKMWKDSFENRRCLIPCLGFYEPHK
                     SQRVPSPHTGKPIKQQYLFDKGGDIFYLAGIYQDKYFSLLTTAPNSHVRSIHDRMPLV
                     VKDQELFLWLGGESEQFLDREEIKLNIRARYPQPLQF"
     gene            complement(4418..5047)
                     /locus_tag="VFD08_04600"
     CDS             complement(4418..5047)
                     /locus_tag="VFD08_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10333.1"
                     /translation="MKKIISTFLLLLLMLGSCASGPAEPQKPDLDQTSPEKTLESFFT
                     AANFMDMEEMIRLINPSKIQAIEEAKDFFAELEGDNYPPSMKDYFHKAAGLITYEILD
                     SQKKEDKAQIHIESHYVESKPLLTNIIKELFSRVLKSAFTGAEIDEEAMIEKVSRDMI
                     PKILEADKVETFTVNLEKYKEDWYFDGLSDEIKDVLTAGFFSLESDEVF"
BASE COUNT         1536 a         1214 c         1044 g         1848 t
ORIGIN      
        1 ggttcctagt tttcttgctc cctttttatc aagaaaaagc aggggaaagt cccttgtgtc
       61 ttttcgtaag tttttaatca tctcttggtc tgtttcatat tcaaattgga ggtctctagc
      121 accaaatttt gaaaggtccc ctctaagcct tggagcatcc agaatgcaaa tagcatcttg
      181 aaataggttt ccatggggac taattaaaac taaagtatca ggttcaagag aagaaaatcg
      241 gcgggccact tcctctatcc cctccagtgt caatttagct tctaaggctt cctctcctcc
      301 aatttcagaa atcaaaatag gtggatgagg aagaagggct gcggctaaag gatacataga
      361 atctcctttc ctaattcgtt tttactaagt ttagattaaa cttgaagtgc ttatgtctat
      421 atttactctt tacccttgtc tggcttgagc tacccaatta gaaagtttag tacattctaa
      481 agaacttgca gttatttgct ataatgaaga cgagaaaatc cccaaggagg aaaaatgatc
      541 ttaataaaaa acattcatgt ctatgctcca gaagatcttg gaattaaaga tgtctttgtg
      601 agtggagaaa aaatcaccct tattgaaaat gaaataccag cttttcataa ggatatcaag
      661 gtaattgatg gcaagggaaa gatccttatc cctggtctaa ttgacaacca tgttcatatt
      721 accggcggcg ggggggaagg aagtttcaaa agtcgggtac ctgaaattac tctttcaaaa
      781 ctccttaaag gagggattac cacagtagta ggtcttttag gtaccgatgg ttccacccgc
      841 aacattgaaa accttgtggc taaagcaaag gctctaaaag aagaaggcct tagtgcctat
      901 gtccatacag gttcctatgc ctatcctccc atcagtctta caggctcccc aggcaaggat
      961 attttatttg ttgaggaggt attgggcgta aagattgctc tttcagatca tcgctcctct
     1021 tctataacaa ataatgaact ggcccggctg gccagtgagg tcagggtagc aggaatgctc
     1081 tcagctaagc ccggcattgt tgtagttcat atgggagagg gaaaaagagg attagagcct
     1141 attctggaaa ttcttgatag tacagacctt cccataaaaa cctttagacc aacccatgtt
     1201 aatcgcaatc cccaactttt aaagcaagcc ctggcttttg caaaaaaagg aggcaatatc
     1261 gatttaacct gtggggagaa aaacgaactt tctactgcca gtgttcttcg acaggcagaa
     1321 aaacttatgg tccctagaga aaatatcacc cttagctccg atggctacgg aagtttttct
     1381 gattatgatc agtttggaaa cctaagccat attggggttg cctcagtttc gattcttttt
     1441 aaagaactca gggatttggt cctggttgaa ggctttgatc tagaaagggc cctaatcttt
     1501 tttaccagca atgtggccaa ggccctccac ctgtatccag acaagggatg tataaaggaa
     1561 aattcccatg cagaccttct tattcttgat aaggacctgg ccctttcttc ccttattgcc
     1621 agaggaagac tccttcttca taaaaaagag cttttagtta aaggcaccta tgaatagggt
     1681 agttttaaaa gaggtttgcc ttagattgca aacctcttat tttttatcca agtttagcta
     1741 aatctactgc caaggcactg cccacccggg cattattata gaccagctct atatttgctt
     1801 ttaaagagtc ccccttggtt actcgcttta gttcatccag tagataaggg gttacttctt
     1861 ttccgctgat accaagttct tttgcatgaa gaagggctgt ttgaatcgac ccctctatga
     1921 gtccatcatc aagcgctgct tcttcagcta taggaacggc caggacagcg cccccttcaa
     1981 gcccaagatc ccatttttct tttaatagcc tggcagcttc ctgggtgctt tccattgtat
     2041 aatcaacaaa gtagccgctt ttgggtgtgt aaaaggaggg gaattccttt gttttatagc
     2101 ccagtactgg caccccatgg gtctctagga cctctaaggt tctgccaata tctaggatgc
     2161 tctttgctcc tgcacatacc accgctactg aggtcttggc tagttccatt agatctgctg
     2221 aaatatcaaa actatcctct cctcccctat ggactccacc aattcccccg gtaacaaaaa
     2281 ccttaatccc agctagcttg gccataatca tggtggctgc caccgtagtc gctccgtttt
     2341 ttttacttgc tagtacatag ggcaggtccc ttcgactgac ctttagggcg tcttttgttg
     2401 tggccaaata ctctaattct tctttattta ggcccacctt cattacccca tctaaaatag
     2461 ccatggtggc aggaaccgct cctgcctggc gaacgattct ttctacttgc agggcagttt
     2521 caaggttttc tggatagggc atcccatgag agataatagt ggactctagg gctacaactg
     2581 ccctgccttg atctagggcc tctttgactt ctttttttat ttcaatcatt tttctaccat
     2641 aaaagacttt ttgtctttac atcctttctt ctttgcctta ttttttcttc ttcaaaatac
     2701 accctatctc tttgctggcc taggacaata agagcgaggg cctgactaaa gcttgctaga
     2761 tgctccaagt cccatccatg atagttggcg tagactgccg cagctaaaaa agcgtctcca
     2821 gctcctgtta cactctttac attttctact tgggcgctag catggataat ttgattttta
     2881 tcggctacaa gcaccccctc ctggcccagg gttagataga cctcttttac gcctagttca
     2941 agaacttgat aaagggcctt tttatagtct tcaaagccgc taagctttct tcctagcagg
     3001 ctctgcatct cataaatgtt aggttttatt gcatagaggt actttagtga gtttttaagc
     3061 tttaggcttt ttaccgttga aactggatcg acaaaaatcc tttggtcttg atggttttta
     3121 catagatagt ctattacttc ttcttgcaag tttgcatcaa gaacaatcat aggagattct
     3181 ttgattagct cttttttttc ttgtaaatac tccttattta gtttttctaa gatgcccatt
     3241 tgatttattg ccaccaccag ctcccctttt ttatccaaaa tagagagata gactgagctg
     3301 ggagtatttt ctaaataaag actatcctgc atatctacgc ctaacttttc tgttgaactc
     3361 cttataaagt ccccttcttt gtcttttcct aaggtcgtaa gtagtctggt agaaagtcct
     3421 agcctggcaa tgttttctgc aatatttcta gctactcccc cagcagaggt agtaagcttg
     3481 cccgggtttg aatcatagag ctttaaatct tggctactaa ttccctgaag gtcaagatta
     3541 caagccccta ttagggtgat tttaggatct gtttttattt ttcctatctt ggcttcttct
     3601 tttttcataa ttccccatat ttctttattt ctatctatgt ctttatatta taagttcatc
     3661 ttaaaaatag caatactttt acttctttta gggctctata aaaaaacagc tcctaaatag
     3721 aagctgcgct tttaaaactg taagggctgg ggatatctgg cccttatatt taatttgatt
     3781 tcctctcgat ctaaaaactg ctcgctttcg ccccctagcc agaggaatag ttcttggtcc
     3841 ttgactacca ggggcattct atcgtggata ctacgcacat ggctattagg tgctgtggtt
     3901 agtaaggaaa agtacttatc ctgatagatg ccagccagat aaaatatgtc tcccccctta
     3961 tcaaatagat actgttgttt gatgggtttt cctgtatgcg gactggggac tctttggctt
     4021 ttatgaggct cataaaagcc caggcaagga attagacacc tgcgattttc aaaggaatcc
     4081 ttccacattt tctttccctt tatggcgctt tcaagacgag tattaaaaac aggccccttt
     4141 tgccaagaaa ccggataacc ccaaagaagt tcaacaagct ctaatccctc tttagctgct
     4201 agataaatag gggctcttat cccgggaaag atatccctat agggatatgg ttttttagaa
     4261 gaaatcttgc ccttatctct ttcttggagc atttctgctc ttggtgcaaa ataatgtaca
     4321 cacatatctt cctccactag ataaataccc caaaaagagg tgaattcact tttatcttac
     4381 ttagtggaag aataaaacta gctagctggc ttatcaccta aaaaacctca tcgctttcta
     4441 aagaaaaaaa gccagcagtt aaaacatcct ttatctcatc gcttagtcca tcaaagtacc
     4501 agtcttcctt gtatttttca agattcacag taaaagtctc gaccttatca gcttctagta
     4561 ttttgggtat catatccctg ctaacctttt ctatcattgc ttcttcatct atttctgcgc
     4621 cagtaaaggc gcttttaagg acccttgaaa aaagctcctt tatgatgtta gtaagcaggg
     4681 gcttagattc aacataatgg ctttctatat gaatctgggc tttatcctct tttttttgac
     4741 tgtctaaaat ttcataggta attaaccctg ctgccttatg aaaatagtct ttcatgctcg
     4801 ggggataatt atcaccttct agctcagcaa aaaaatcctt tgcctcctct atggcttgaa
     4861 tctttgaggg gttgattagt cttatcatct cttccatatc cataaaattt gcagcagtaa
     4921 aaaaagattc cagggttttt tcagggcttg tctgatcaag atcaggcttt tgaggctctg
     4981 caggcccgct ggcacatgag ccaagcataa gtagtaggag caagaatgta gatattattt
     5041 ttttcatttt gtatttcccc tttttaaatg aactttaaac ttattacaat tataccataa
     5101 tttatgtctt cttttatcca tatagagctt tgatcttagc ttatttcttc ttgataagaa
     5161 atctagcttc tggcttattt attaattaac ttgaatcctt tctcaatctc gattacacta
     5221 taattaaaga caagagggtg agcttgtgag cgtcttctta ggtaagacta gaagaagggc
     5281 aaaaactatc ccttgatttt tacagctcag ctggtttttc aggtgactgg gtattttcta
     5341 ggataagtga ctctagagtc tttagagcaa ctattagaag ctaaaataga ctgcctaggc
     5401 agtctattct tttcattttc attacaataa acctgcttac aactgattta atttcctgct
     5461 tgaagtctcc ctaccttatt aaagcaggta aaaaacttgc tctagatcgc tgccaatctt
     5521 ccttctagga aaactcaaaa gaaaaacata aataaattct gtaaaattag actttttttt
     5581 actggttaca ataacacatt attgtttaag actatttaca aagataacag gtggtactta
     5641 tg
//
LOCUS       DASRNA010000168         7867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_572667,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000168 DASRNA010000000
VERSION     DASRNA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572667"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFD08_04605"
     CDS             <1..784
                     /locus_tag="VFD08_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10334.1"
                     /translation="QSIDEILLQDIDFWFELTPTDLSIASEVHGDISKILRRGIPVIF
                     ANKAPLVYDYLSLKKLADSRQVHLGLSGVLGASLPSYALVHYGSLGAKILSMEGILNG
                     TTNFILKQMEENISFDKAVALAQKLGIAEPNWAYDVDGFDSAIKMSLLASVISNKNIS
                     FDPSHIKGIRDIDLEEIQSLKKENKRIKLLATFEKDIIRVSPKVYTQEDIFHHIDGSD
                     KILYIRTDKLSDMTLIGGKSGLKEVAASLHRDLLWIKELMGH"
     gene            complement(1298..2392)
                     /locus_tag="VFD08_04610"
     CDS             complement(1298..2392)
                     /locus_tag="VFD08_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZK10335.1"
                     /translation="MNRDLLYIMYGSEPKKMVQRALEETNILEDIPHKNSLIGIKPNL
                     VVAKPAELGATTSPELVEGVIQYLTCNGYHNLLILEGSWVGDRTSLAFEVCGYEELSK
                     KYDVPLIDLQKDKYRNYQVEETTISVCKQAMDLDYLINMPVLKGHCQTNITCALKNMK
                     GCIPDTEKRRFHTMGLHRPIAYLNKVLKQDLIIVDGIIGDLNFEEGGNPVQMNRIILG
                     KDPVLVDAYVAQLLGYHIKEIPYIEMAEELKIGSSQIDDANIKELNRDKSPMTIAKSR
                     SIQELAKYIEEDMACSACYGSLIHGLDRLKDRGYLCHIEEKIHIGQGYKYKEGKGIGI
                     GLCTKGFEKNLPGCPPKAKDIISFLLKSID"
     gene            2746..3240
                     /locus_tag="VFD08_04615"
     CDS             2746..3240
                     /locus_tag="VFD08_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10336.1"
                     /translation="MKKIIITTGDESGGIFEEEFMDDKQIIAYLEDERAGGKRWIKAW
                     VQEEENIFRDYEDPKRRRSIPYGAIQVDAKKILQDKEGHIIGHYRVDQSTPIHIFYQG
                     KPYTEAMLEKDPHLKNRIDDILPDFRKGRNEIQNGKEVLERWDIRLEREIKSVVYYQE
                     YSHK"
     gene            3340..6957
                     /locus_tag="VFD08_04620"
     CDS             3340..6957
                     /locus_tag="VFD08_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZK10337.1"
                     /translation="MIRYSIEKKYTTLVLVVIILVLGYVSYTGLPLDLLPDLELPMLV
                     IMTQDPGQTPEAMEAKITRPLEKLLSSTEGLEELQSISSSGRSLLLLSFVQGHNMDSA
                     MIDVSGKIDFLAELWKNTSHPTILRLNPSMLPVMTAAIYDENISAKELTELYEENLQS
                     KLEGTDGVASVGASGLFREEINVFLSEEKVHKINEEILNKVQSSIEEKEGELEEAKEK
                     IQAGKDQLSSASIPTTSPSLPDDLFESSISLASELAELSLNRASNQVYLSSLEQVLLL
                     LQTPAEDLSQTQALIVQYLKDWPFELSEENIENAILWTEDEIIKASEQEALYQAEEAA
                     LQQKLDQVLVSLESMSAAQGQMIAQAGNMLLQAQAAASELTKMEVMLESQAMMLESQK
                     EEALATANISKLLNVDSLSKLLLAQNISFPIGYIKSEDGLIPLTIDNPLTEEELPDLL
                     LTDTGEEAIGKIYLKDISNISNSDNSKDYYAKINGKNAILVNIEKQSQANTSEVSSNL
                     ALTIEEMEKTNPGLEISPLMDQGLYIRWVIEAILKNILIGALFAMAVLYLFLRDIRFT
                     LLIALSIPLSIVTSIILMYFSNISLNLISLAGLALGVGMMVDNSIVVIENSHRLSLLG
                     YRPKEAALEGAREMAPAITASTMTTAFVFLPILFTQGMARELFTDMALTIAFSLFASL
                     FVALTVIPAVSSIRPYVKEKKTKESKYYQNTLLWSLKHPYLVVLASLLLLGGSIFALL
                     EKGAIFIPDMDTPQINLTVNPEDASQSTQELYDISDEVFLRLEDLEDLELIGGLSSQN
                     LMGSSSGISFHLVRKDSSRKSSLELEKEITDRLADLPAHIQVQTQNVNIDAMGGGGLS
                     LWVLAQDLEEARAYSEKLLEDMEAIPSLKEAYIDEEETEDEFHLVIDREKAMEKMLTP
                     AEVYMQLENRLSLPKPIFQLDSLPLIVTEEGEERLNLSELLNSKIQNNNGDEIRLSSI
                     GSIEKATSQVTIRRSFQKRYLEIKGDIEPDSNITLVSREIDKLIENRPPPTGVELHFS
                     GERDQIDSAMNDLRKMMIMAFIIIYGIMIIQFQSLLAPFIVMFTIPLAFTGGALALLL
                     FGKPLSIIAMLGFLILSGIIVNNGIVFIDTARRGKGPIDEVLVNTGKVRMRPILMTAL
                     TTMLGLSPLAMAVGLGSEALQPIGIVIIGGLIYATLMTLYIIPVLYKLLLGGKYAKDT
                     L"
     gene            6938..7657
                     /locus_tag="VFD08_04625"
     CDS             6938..7657
                     /locus_tag="VFD08_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK10338.1"
                     /translation="MQKTLFDRKSVRSYLDREVEQEKKELILTAALRSATAGNMSLYK
                     IIDIKDQKIKDQLAISCDNQPFIATAPLALLFLVDYSGWYKLFKEKDPACKKPGMGDL
                     FLGASDALIAAQSAAICAQSLELGSCYIGDILEQAELHKEIFKLPQYTMPISLLLIGY
                     PSQQQIDRPQPPRFSKEAMVFSDVFEERSLNQLKEDLRNKNPRLGAEAQIDDLFHRKW
                     TSDFLLEMERSVEKWLSWWDN"
     gene            complement(7654..>7867)
                     /locus_tag="VFD08_04630"
     CDS             complement(7654..>7867)
                     /locus_tag="VFD08_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10339.1"
                     /translation="ERDILLLKTVYTIIGLTMLLVIQIVPFPSIEQRFRISLLIFVVI
                     FAVGFNATRNLKTIYKKKEEKKDKKN"
BASE COUNT         2442 a         1628 c         1451 g         2346 t
ORIGIN      
        1 ccaaagcata gacgaaatcc tcttgcagga tattgatttc tggtttgaac ttacgccaac
       61 agatttatcc attgccagcg aagttcatgg ggacatttca aaaatcctta gaagaggaat
      121 acctgttatc tttgccaata aagcacccct agtttatgat tatctatcgc taaaaaaatt
      181 agccgatagc aggcaggtcc accttggtct tagtggggta ctcggtgcct ctttaccgag
      241 ctacgcccta gttcactatg gtagcctagg tgcaaaaata cttagtatgg aaggtatcct
      301 aaatggcacg accaacttta ttttaaagca aatggaagag aatatctcct ttgataaggc
      361 agttgcttta gcgcaaaaac tgggtattgc tgagcccaac tgggcctatg atgttgatgg
      421 ttttgatagt gccatcaaga tgagcctttt ggcttcagtc atttctaata aaaacattag
      481 ctttgaccct agccatatta aagggattag agatatagac ctagaagaaa tccaatcact
      541 aaaaaaagaa aataaaagga taaaacttct agcgaccttt gaaaaggaca taataagggt
      601 tagtccaaaa gtttatacac aagaagacat cttccaccac atagatggat cagataaaat
      661 cctttatata agaacagata aactatctga tatgacccta ataggaggaa aatccggtct
      721 taaagaagta gccgcttcct tgcaccgcga tttgctttgg ataaaagagc ttatggggca
      781 ctagatagat ccttcgtaat ttagcaaatc gcttcaattc tttttacaat aacctcttta
      841 ggcggatgct atgactttca ataacaagcc tctctagcat ttgttgtttt atggccacct
      901 ttaaaaagcc catcacacca acaatagtat ttgcttaaaa catttgaaag agtgttttat
      961 gcatttatta gattggcaaa acagggggac tacttattcc tagacccgtt tttagggctg
     1021 ttttcagagc caagtattat atttcaagag ccccaaaaaa cacagcttca aataataccc
     1081 acttattgat tggtgatctt gatggcaact cgcattatct aaccttcttt ttagataaag
     1141 gagatgacac aaaaaagctc aagattatat ggagggattt attggtgaga cagatggtgg
     1201 cctttgtatt tgaatcagaa aacagaaaag acaaaggcta ctgacttgat gaaaaatcaa
     1261 gatcaagggt aaaatcttat cctagtggac ctccccttta atctatcgat tttaacaaaa
     1321 agctaatgat atctttggcc tttggtgggc aaccaggcag gtttttttca aaacccttag
     1381 tacataatcc aatccctatt cccttgcctt ctttatactt atagccttgc cctatgtgaa
     1441 ttttttcctc tatatgacaa agataccctc tatcttttaa cctgtctaat ccatggatta
     1501 gactgccata acaagcagaa cacgccatat cttcctctat atattttgcc agttcttgaa
     1561 tggacctgct cttggctatc gtcatgggtg atttatcccg atttagttcc ttgatattgg
     1621 catcatcgat ttgactgctt cctatcttca attcttctgc catttctata tagggaatct
     1681 ctttaatatg gtagcccaat aattgagcta cataagcgtc taccaaaaca gggtcttttc
     1741 ccaatataat ccgattcatt tgaacagggt ttcccccttc ttcaaaattc aagtccccta
     1801 tgattccatc aacgataatt aaatcttgtt ttaacacttt attcaaatag gcaataggcc
     1861 tgtgaagacc catggtatgg aagcgccttt tctctgtatc gggtatgcaa cctttcatgt
     1921 tttttaatgc acaagtaata ttcgtctgac aatggccctt taaaacgggc atattgatta
     1981 agtaatccaa atccatagcc tgcttacata cagagattgt cgtttcttct acttggtagt
     2041 tcctatattt atccttttgc agatctatta aagggacatc atatttctta gatagttctt
     2101 catacccaca tacttcaaag gctaaggagg tcctatctcc cacccaagat ccttccaata
     2161 tgagtaaatt gtgataacca ttgcaagtaa gatattgtat cacaccttca accagctctg
     2221 gggaggtagt agctcctaat tcagcaggtt ttgccaccac tagattaggt ttaatgccta
     2281 tcaatgaatt tttatggggt atatcctcta atatattcgt ctcttctaag gctctttgaa
     2341 ccattttttt tggctcgcta ccgtacatga tatatagtaa atctcgattc ataaaaatcc
     2401 ccttcctaca aagtatcata ttaagtgttt ttcttagaat ttaatgttat tgtagcatat
     2461 cccactggcc ggcttcgctt caactcgtct tattactcct tcgagttttt tcttttgtcc
     2521 gatttaaatt taacttacta acttttatca ccccaccttt tatatccagc gctttaaccc
     2581 aggatatttc ttgtgttttg agagatagac atcaattaat cagcatcttt tgaattattt
     2641 gtaacataga ttgacacttc tttttgtcta ttgtataata ttagtgagca tataagctca
     2701 aaattgttgc ctactccaaa ggtctcttat accaaggggg aaaatatgaa aaaaatcatt
     2761 attacaacag gcgatgaaag cggcggtatt ttcgaagaag aatttatgga tgacaagcag
     2821 attatagctt acttagaaga tgagcgtgct ggtggaaaac ggtggattaa agcatgggtt
     2881 caagaagagg aaaatatctt tcgagactat gaagatccaa aacggcgacg atcaatcccc
     2941 tatggggcca ttcaagttga tgcaaaaaaa atattacagg ataaagaagg acatatcatt
     3001 ggtcactacc gcgtcgatca atctactcca attcatattt tttatcaggg aaaaccttat
     3061 actgaagcta tgctagaaaa ggatcctcat ctaaaaaacc gtatcgatga tatcttacct
     3121 gatttcagaa aaggccgtaa tgaaattcaa aatggcaaag aagttcttga acgttgggat
     3181 ataagactag agcgagaaat aaagtcagtt gtctattatc aagaatactc acacaaataa
     3241 aaaatacaga aaaaggctta tttctaggcc tttttctttt tttaggcaca atataatttt
     3301 tttcctaatt ttagggtaga atcaatagca ggaggcatta tgattagata cagtattgag
     3361 aaaaaatata ctaccctagt tcttgttgtt attattttag tgctaggtta tgtatcatat
     3421 accggcttac ccctagatct cctgccagat cttgagctgc ctatgttagt tattatgacc
     3481 caggatcccg gtcaaacacc tgaagccatg gaggccaaaa taacaagacc cttagaaaag
     3541 cttctttctt ctactgaagg tcttgaagag ctccaatcca tttcctcttc aggtcgttcg
     3601 ctcttgcttt taagctttgt ccagggccat aatatggatt ctgctatgat tgatgttagc
     3661 ggaaaaatag attttcttgc agagctttgg aaaaacacca gtcatcctac gatactaagg
     3721 ctaaatccct ccatgttgcc tgttatgaca gcggctattt atgatgaaaa tatatctgcc
     3781 aaagaattaa cagagctata tgaagaaaat cttcaatcaa aacttgaagg aaccgatggc
     3841 gttgccagcg taggagcctc ggggcttttt cgcgaggaaa taaatgtctt tctctcagaa
     3901 gaaaaagttc ataagataaa cgaagagatc ctaaataagg ttcaaagttc catcgaggaa
     3961 aaagaaggtg agttagaaga agcaaaagaa aaaattcaag cgggaaaaga tcaactatcc
     4021 tcagcaagta ttcctacaac tagcccctcc ctacctgatg acctttttga atctagtatt
     4081 tcacttgctt cagaacttgc agaacttagc cttaaccgag ccagtaatca ggtctatctt
     4141 tcctctctag agcaagttct tctccttctt caaactccag cagaagatct tagccagact
     4201 caggctctaa tagtacaata cctaaaagat tggccctttg aactaagtga agaaaatata
     4261 gaaaatgcta tcctctggac agaagatgaa attataaagg ctagtgagca ggaagcactt
     4321 tatcaagcag aagaggctgc cctgcagcaa aaactagacc aagttcttgt ttccttagaa
     4381 agcatgtccg ccgctcaggg gcaaatgata gcccaagctg gaaatatgtt gctccaagcc
     4441 caagctgcag ccagcgaact taccaagatg gaagttatgc tggagagcca agccatgatg
     4501 ttagaatcac aaaaagaaga ggctctagcc acggccaata tttcaaaact tctaaatgta
     4561 gattctcttt caaaacttct tctggcccaa aacatttcct ttcccattgg ttatataaag
     4621 agtgaggatg ggcttatccc tcttactatc gataatccct taactgagga ggaattgcca
     4681 gacttactct taactgatac aggcgaagaa gcaataggaa aaatctacct aaaggatatt
     4741 agcaatatat ctaatagcga taattccaaa gattattatg caaagatcaa tggaaaaaat
     4801 gctatcctgg taaatataga aaaacaatct caggcaaata catcagaggt ttcaagcaat
     4861 ctagccctta ctatagaaga aatggaaaaa accaacccag gcttagaaat tagtcctctt
     4921 atggaccagg ggctttacat ccgttgggtc attgaagcca ttcttaagaa catccttatt
     4981 ggcgccttat ttgctatggc tgtactctat ctattcttaa gagatattcg ctttaccctg
     5041 ctaattgcct tgtccatccc actttctatt gtcacatcta ttatccttat gtacttttcc
     5101 aatataagtt taaatctaat ttctctagcg ggacttgcct tgggagttgg tatgatggtg
     5161 gacaactcta ttgttgtaat agaaaactcc caccggctta gccttcttgg ctataggcct
     5221 aaagaggccg ctctagaagg ggcccgtgag atggctccag ctatcaccgc atctactatg
     5281 acaactgctt ttgttttttt acccatactc tttacccagg gaatggcaag agaactcttt
     5341 actgatatgg ccttaaccat agccttttct ttatttgcta gcctttttgt ggctcttact
     5401 gtaataccag ctgtaagcag catccggcct tatgttaaag agaaaaaaac caaagaatcc
     5461 aaatactacc aaaacaccct cctttggagc ttaaaacatc cctatcttgt tgttttagcc
     5521 agccttcttc ttcttggggg aagcatcttt gccctcctag aaaaaggggc catctttatt
     5581 cctgatatgg acacccctca gattaacctc actgttaatc ctgaggatgc atctcagagc
     5641 acacaggagc tttatgacat atccgatgag gttttccttc gccttgaaga tttagaagat
     5701 ctcgaactta taggaggtct aagcagtcaa aacctaatgg gaagttctag tggcattagt
     5761 ttccatcttg taagaaaaga cagctccaga aaaagcagtc tagaattaga aaaagaaatc
     5821 acagatcgcc tagcagacct tccagcccat attcaagtgc agacccaaaa tgtcaatata
     5881 gacgccatgg gaggaggggg cctaagtctt tgggttcttg cacaagatct tgaggaagct
     5941 agagcctata gtgaaaagct tcttgaagat atggaggcca tccctagctt aaaagaggcc
     6001 tatatcgatg aagaagaaac cgaagatgaa ttccatcttg tgattgatcg agaaaaagcc
     6061 atggaaaaaa tgttaacacc tgccgaagtg tatatgcaac ttgaaaaccg cctttctctt
     6121 cctaaaccta tttttcagct agatagcctc ccccttattg ttactgagga aggagaagag
     6181 cggcttaatc ttagtgagct actcaatagc aaaatacaaa ataataatgg ggatgaaatc
     6241 agactctcct ctattggaag cattgaaaaa gcaactagcc aggtaacaat acgaagatct
     6301 ttccaaaaac gatatttaga aataaaaggc gatattgagc ccgatagcaa tatcaccctt
     6361 gtttcaagag aaattgataa gctaattgaa aataggcccc ctccaacagg agtggaactt
     6421 cacttttccg gggaaagaga tcagatagat tctgccatga atgatctgcg aaaaatgatg
     6481 attatggcct ttattatcat ctatggcatt atgattatcc agtttcagtc tctgctagca
     6541 ccctttatcg ttatgtttac cattcctcta gcctttactg gaggcgctct ggcccttctt
     6601 ctttttggta aaccactcag catcattgcc atgcttggtt ttcttattct aagcggcatt
     6661 atcgttaata atggaatcgt ctttatcgat acagccagaa gaggaaaagg acccatagat
     6721 gaggtgctag taaatacagg aaaagtccgt atgcgcccta tcttaatgac ggcccttaca
     6781 actatgctgg gactttctcc cctagctatg gcagtaggtc ttggttctga agctctccag
     6841 cccataggga tagtcattat cggcggttta atttacgcca ctttaatgac cctttatatt
     6901 atccctgttc tctacaaact tttacttgga ggtaaatatg caaaagacac tctttgatag
     6961 aaaatctgtt cgttcctatc tagacagaga agttgagcaa gaaaaaaaag agcttattct
     7021 tacagctgct cttcgctctg ccaccgctgg caatatgtct ctatacaaaa ttattgatat
     7081 taaggatcaa aaaataaaag accagctggc cataagttgt gataaccagc cctttattgc
     7141 cacggctccc ctagcacttc tttttctagt agactacagt ggctggtata aactttttaa
     7201 agaaaaagat cctgcttgca aaaagcctgg tatgggagat cttttcctgg gtgcatcaga
     7261 cgctcttatt gcagcccaga gcgctgctat ctgtgcccag agcctagaac ttggaagctg
     7321 ctatataggt gatattcttg agcaagccga gctccacaag gagatcttta agctgcccca
     7381 gtataccatg cccatatctc ttcttcttat tggctaccca agccagcaac aaatagatag
     7441 acctcagcca ccacgctttt ctaaagaagc tatggtcttt agcgatgtct ttgaagaaag
     7501 atctctcaac cagctcaaag aagatcttcg caataaaaac ccccgccttg gggcagaggc
     7561 tcagattgat gatctatttc atcgaaaatg gacatcagac tttcttctag aaatggagcg
     7621 cagtgtggaa aaatggcttt catggtggga taattagttc ttcttatcct tcttttcctc
     7681 ttttttctta tagatggtct taagatttct tgttgcatta aagccaacag caaaaatgac
     7741 cacaaaaata agaaggctaa ttcgaaatct ttgttcaatc gatggaaaag gaacaatctg
     7801 gataacaaga agcattgtaa gaccaataat ggtatatact gtttttaaaa gaaggatgtc
     7861 cctttct
//
LOCUS       DASRNA010000169         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_647781,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000169 DASRNA010000000
VERSION     DASRNA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647781"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFD08_04635"
     CDS             <1..751
                     /locus_tag="VFD08_04635"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HZK10340.1"
                     /translation="LYNYFGWERPYFVHLPNILNTQRKKLSKRQGDVSVSDFREKGYL
                     PEALVNFLALVGWSPGDEKEIMTMDEMIDAFSFERVSRSGGVFDIDKLNYINNHYIRQ
                     AENKRLYDLLPQELFESREEEHILEIIELVKEKVDRVVDLIPFIEDFYTDELIVEEGS
                     LETLKEPHVAELLELFASKLQGLENYDKENIQKSIKECQKELKVKGKQLFMPLRIAIS
                     GTTHGSDLVKTIALLGRKRALSRLSQAREMV"
     gene            748..2130
                     /gene="cysS"
                     /locus_tag="VFD08_04640"
     CDS             748..2130
                     /gene="cysS"
                     /locus_tag="VFD08_04640"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZK10341.1"
                     /translation="MKLKLYNSLHLEIEEFVPLSDKRVSIYTCGPTVYNYAHIGNMRT
                     YVSEDILVRTLEALGYNVLRAMNITDVGHLTDDGDDGDDKMLLGAKREKKTVWEIADY
                     YTEAFFKDFDELELKRPDIVEKATDHIDGYIDFIKVLEEKGFTYQAGGNVYFDISKFP
                     RYNELSRMPLENLQVGSREGVFRDDNKKNPFDFVLWFTKSKFEDRQMRWKTIYGEGYP
                     GWHLECSVIAMDAFGPRMDIHCGGVDHIPTHHTNEIAQSESYTGEKWVNYWWHAEWLI
                     DNEGKMSKSRGEFLSLQYLKSLGYDPMDFKYFLLGSHYRRQLHFSFEALDGAKDAYTK
                     LKARCLALDPPEKTSAYWEEFLGHLAMDLNTANALTVLYDCLKDPDLSSGEKAYAIRR
                     MDTVLKLALFEEKKIDKDLKEEIQDLVDQRSKAKADKDYGKADKLRDKLEAMGVSIKD
                     SKEGTTWQLH"
     gene            2115..2525
                     /locus_tag="VFD08_04645"
     CDS             2115..2525
                     /locus_tag="VFD08_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HZK10342.1"
                     /translation="MATALAPVNRYNSLQLAYLGDAVYELYIREYLLASGFMSPGELN
                     RRAKAYVSARAQSKAYFEVEKEVTSQEENYLRRGRNAKGGAAPSSASISDYRRATGLE
                     SLLGALYVMEKKERITEIMDICIRVLEENNEKLV"
     gene            2509..2814
                     /locus_tag="VFD08_04650"
     CDS             2509..2814
                     /locus_tag="VFD08_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10343.1"
                     /translation="MKNWSKNERRLFSFSLVLLLLLSGKSVFFDGYKALDKKESKVMQ
                     LALEETEVKPWIHKKVVKVKALDPQHYENISMTHGYLVVVRKYFLGIVPIGESRVLE"
     gene            2814..>3516
                     /gene="rlmB"
                     /locus_tag="VFD08_04655"
     CDS             2814..>3516
                     /gene="rlmB"
                     /locus_tag="VFD08_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZK10344.1"
                     /translation="MDVIIGKNPVLEGLKSSLQINKVSFYKNMGRKDIEEIILLCEEK
                     KIPYTWVEREKLDEHGKPHQGVVAFVSSISYASLYSLIHKEGLILMLDGVTDPHNLGA
                     IIRSAHVFGAQGLVLPKRRSASLDATSFKASAGAAAHLPIARVANLNDAIKEFKEKGF
                     WVYGAQAEAAHELSQIKFPKKTVLVFGSEDKGISRLVKDGCDELYKIPMANFESLNVS
                     VASGIACYSYFLQNPV"
BASE COUNT         1113 a          608 c          811 g          984 t
ORIGIN      
        1 cctttataat tattttggtt gggaaagacc ttattttgtc cacttaccca acattttaaa
       61 cacccaaaga aaaaaactca gcaaacgaca gggtgatgta tctgtatcgg attttcggga
      121 gaaaggctat cttcctgaag ccctggtaaa ctttttagcc ctagtaggct ggagtccagg
      181 cgatgaaaaa gaaattatga ccatggacga aatgatcgac gcctttagct ttgagcgggt
      241 ttcgcgtagt ggtggggttt ttgatattga taaactcaat tatattaaca accactatat
      301 tcgccaggct gagaacaaaa gactttatga tcttcttccc caggagttat ttgaatctag
      361 ggaggaagaa catatattag aaattatcga actggtaaaa gagaaggtgg atagggtagt
      421 cgatttaatc ccctttattg aagattttta tactgatgaa ctgatagtag aagaagggtc
      481 tttggagacc ttaaaagagc cccatgttgc tgagctgcta gaattattcg ccagcaaatt
      541 acaagggctt gaaaactatg ataaagaaaa tattcaaaaa tcaatcaaag agtgccaaaa
      601 agaattaaag gtaaagggca agcaactttt tatgcctcta agaattgcca ttagtggcac
      661 cacccatgga tcagacttgg taaaaacaat tgctctttta ggtagaaaaa gggcactttc
      721 tcgattaagc caagcaaggg agatggtatg aaattaaaat tatataattc tctgcatctt
      781 gaaatagaag aatttgttcc ccttagcgat aagagagtga gcatttacac ctgtggtccc
      841 actgtatata attatgccca tatcggcaat atgcgcacct atgtaagtga agacattctt
      901 gtaaggaccc tagaagccct tggctacaat gttcttaggg ccatgaacat tactgatgtg
      961 ggtcatctta ctgatgatgg ggatgatggg gatgacaaga tgctccttgg agccaaaaga
     1021 gaaaaaaaga cagtgtggga aattgcggat tattatacag aggccttttt taaagatttt
     1081 gatgaactag agctaaaaag gccggatata gtagaaaaag caacagacca tattgatggc
     1141 tatattgatt ttattaaagt cttagaagaa aagggcttta cctatcaagc tggtgggaat
     1201 gtttattttg atatcagtaa atttccccgc tataatgagc tttcacgtat gccccttgag
     1261 aatctacaag taggctctag ggagggtgtt tttcgcgatg acaataaaaa gaatcccttt
     1321 gactttgtac tatggtttac taaatcaaag tttgaggacc gacagatgcg ctggaaaacc
     1381 atttatggag aaggttatcc cggttggcat ttagagtgtt ctgttattgc aatggacgcc
     1441 tttgggccac gaatggacat tcactgcggt ggagtggacc atatccctac ccatcacacc
     1501 aatgagattg cccaaagtga aagttatacg ggagaaaaat gggtcaacta ttggtggcat
     1561 gcagaatggc tcattgataa tgaaggaaaa atgagcaaat caagaggaga attcttgagc
     1621 ctacaatatc taaaaagtct tggttatgat ccaatggatt tcaaatattt tttactggga
     1681 agtcattata gacgacagct tcactttagt tttgaagctc tggatggagc taaagatgcc
     1741 tatacaaagc ttaaggctag gtgccttgct ttagatcctc ctgaaaaaac atcagcatat
     1801 tgggaagaat ttctaggtca tttggcaatg gatctaaaca cagccaatgc ccttacggtc
     1861 ctatatgact gtttaaaaga tccagatctt tcttctgggg agaaagctta tgccataaga
     1921 cgtatggata cagtgctaaa attagcactc tttgaagaaa aaaagataga taaagacttg
     1981 aaagaagaaa tacaagattt ggttgatcag cgcagcaagg ccaaggcgga taaagactat
     2041 ggcaaagccg ataagcttcg tgataagctt gaagccatgg gtgtaagcat caaagacagt
     2101 aaagaaggaa caacttggca actgcactag ctccggtgaa ccgctataac tctttgcagc
     2161 ttgcttattt gggcgatgca gtctatgagc tctacatccg tgagtattta ctcgcaagtg
     2221 gttttatgag cccgggtgag ctaaatagac gggccaaggc ctatgtcagt gcaagggccc
     2281 aatcaaaggc ctactttgag gtagaaaaag aagtcacttc ccaggaagag aactatttgc
     2341 gccggggaag aaatgccaag ggaggagctg ccccttcctc ggctagtatt agtgattatc
     2401 gcagagcgac aggtctggag tctttacttg gtgctcttta tgttatggaa aaaaaagaac
     2461 gcattacaga aattatggat atttgcatta gagtgttaga ggaaaataat gaaaaactgg
     2521 tctaaaaatg aaagacggct cttttctttt agtcttgtgc ttttgctcct tctaagcgga
     2581 aaaagtgttt tttttgatgg ctataaagcc cttgataaaa aagaaagcaa ggtaatgcag
     2641 ctggctcttg aagagactga ggtaaagcct tggattcata aaaaggtggt aaaggtaaag
     2701 gcccttgacc cccagcatta tgaaaacatc agtatgactc atggttactt ggttgtggta
     2761 cgaaaatact ttttaggcat tgtgcctatt ggagaaagta gggtattaga ataatggatg
     2821 taattatagg aaaaaaccct gtccttgaag gactcaaaag ttctttacag attaacaagg
     2881 tttcctttta taaaaatatg ggccgtaaag atattgaaga aatcattctt ctttgtgaag
     2941 aaaagaagat tccctatact tgggtagaaa gagaaaaact tgatgagcat ggcaagcccc
     3001 accaaggggt agttgccttt gtaagcagta ttagttacgc cagtctttat tccttgattc
     3061 ataaagaagg cctaatacta atgcttgatg gggttacaga tcctcataac ctaggagcaa
     3121 ttattcgaag cgctcatgta tttggcgctc agggactggt cctgcccaaa cgacgttctg
     3181 cgagtttgga tgctactagt tttaaggcca gtgctggagc tgccgcccat cttcctattg
     3241 ccagggtggc caacctaaat gatgcaatta aagagttcaa agaaaaaggc ttttgggtct
     3301 acggcgctca ggcagaagcg gctcacgagc tttcacagat caagtttcct aaaaagacag
     3361 ttttagtctt tggctctgag gacaagggaa ttagtagact tgttaaggat ggttgtgatg
     3421 aactgtataa gatacctatg gctaactttg aatcccttaa tgtttctgtt gcctcgggta
     3481 tagcatgtta tagttatttt ttacaaaacc ctgtgg
//
LOCUS       DASRNA010000170        20903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_51021,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000170 DASRNA010000000
VERSION     DASRNA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 20903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20903
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51021"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFD08_04660"
     CDS             complement(<1..457)
                     /locus_tag="VFD08_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK10345.1"
                     /translation="MIFEEYGKQLFELISDHTQLGIAVIGVDGRFLYYNRALEEMEGL
                     ETGEMLGKHVLDVFPSSDPQTSTILQCIETGKPIYDDLQTYINFKGRKVTSIVTNIPI
                     IIKGEIKGVLEIIYNANRIKQIYESTSSLFEDEEDAGKSRAKHLKNSYYV"
     gene            complement(605..681)
                     /locus_tag="VFD08_04665"
     tRNA            complement(605..681)
                     /locus_tag="VFD08_04665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Arg,seq:tcg)
     gene            complement(799..875)
                     /locus_tag="VFD08_04670"
     tRNA            complement(799..875)
                     /locus_tag="VFD08_04670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(839..841),aa:Arg,seq:cct)
     gene            complement(884..959)
                     /locus_tag="VFD08_04675"
     tRNA            complement(884..959)
                     /locus_tag="VFD08_04675"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:His,seq:gtg)
     gene            complement(973..1049)
                     /locus_tag="VFD08_04680"
     tRNA            complement(973..1049)
                     /locus_tag="VFD08_04680"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1013..1015),aa:Pro,seq:tgg)
     gene            complement(1127..1573)
                     /locus_tag="VFD08_04685"
     CDS             complement(1127..1573)
                     /locus_tag="VFD08_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZK10346.1"
                     /translation="MKVLVISDSHGDKKTVDEIVKKEKPDLSLHLGDYGKDLKEGLSV
                     RGNCDGMSAAPLRRVFELKGQRIMMSHGHKEGVKRDLHNLFYRAKEERADIVLFGHTH
                     QPLKRTIDQILFLNPGSVSLPPPGKRGTYLILQIQEAGVEVIVKEV"
     gene            complement(1551..2144)
                     /gene="rdgB"
                     /locus_tag="VFD08_04690"
     CDS             complement(1551..2144)
                     /gene="rdgB"
                     /locus_tag="VFD08_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZK10347.1"
                     /translation="MKTIVVASQNPGKIEEIRYILRNWKVKFISLAELGFNREIEENG
                     KSFRQNALIKAREVRKFTDLPVLSDDSGIIVEALGGGPGIHSARYGGDISFEEKRKKM
                     LEEMKGLENRNAFFACEIAFLVDDKEFTQLGLCFGEIALEERGELGFGFDSIFYYPSL
                     NKSFGELSMEKKHSISHRWDALRRLEEKLKNEGISYL"
     gene            complement(2107..2817)
                     /gene="rph"
                     /locus_tag="VFD08_04695"
     CDS             complement(2107..2817)
                     /gene="rph"
                     /locus_tag="VFD08_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZK10348.1"
                     /translation="MRRIEFICGYTKHAHGSVLAVFGQTKVLCTVYAVEGVPKFRPNG
                     EGWLTAEYSMLPGSTFTRKQRRTISGEIDGRTIEIQRLIGRSLRLALDFSLLGPMTLY
                     VDCDVLQADGGTRTAAISGAYLALRLAMDDLMQKGYLHSNPLQRKLAAVSVGIVDAQV
                     ITDLSFQQDSRAEVDLNVVMDEQGNFIEVQGTAERSTYSRAQLHTMLDLAAEGIEEII
                     RKQDQSYENYSSGQPEPW"
     gene            complement(2821..3792)
                     /locus_tag="VFD08_04700"
     CDS             complement(2821..3792)
                     /locus_tag="VFD08_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HZK10349.1"
                     /translation="MYIGKLATEDLNQLVLSKIKNHRSTTKMGPGVGLDVAYLQFGEK
                     LMAVASDPITGAGKNIGSLALLIACNDISAGGGEPLAVMITMLLPPHIKKEEIAEIME
                     DAQTMATKLNVDILGGHTEVTTAVNRVVLSLTALGRVERPLKGIQENDAIVMTKSLGL
                     EGVSIIVAEKEEIRAHLTKEEIEDAQQMGQQLSIAKEARIGLDHGVNFMHDITEGGLV
                     GALSEMCFESNLGFFVDSKKVPIHDVTHKVSDFYDIDPFQLISSGSLLMTLPKEEVEG
                     LLQELSAKGIEASHIGEFRTDQRKILRFDQDVEVEMKAGDELYKVMG"
     gene            complement(3802..5772)
                     /gene="ligA"
                     /locus_tag="VFD08_04705"
     CDS             complement(3802..5772)
                     /gene="ligA"
                     /locus_tag="VFD08_04705"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZK10350.1"
                     /translation="MIKEKYQNLLEQLKKHSHLYYVLDSPEISDQNYDLLYKEVEALE
                     KEHPELLDPSSPTQRVGGELLSGFVKVPHPKFLGSLDNSYNEEDLIKFSRRVENIEPR
                     PSYVLEEKVDGLSVVLHYHKGRFVLGATRGDGLQGEDVTQNLRTIGAIPLELSEPVDL
                     IVRGEVYLPKKNFIQLNKQQEEAGLAPFANPRNAAAGSIRQLNSKLMAKRGLSIWIFD
                     LLEGPSFSHHDKKLDYLRSLGFRVISVEVFEKMRAIIDRIPIYEEEKNKKDFEVDGLV
                     IKVNEEALQRSLGETSKAPRWAIAYKFAPERSQTKLLDVVWTVGRTGVVTPTAVLAPV
                     QLAGTKVQRASLHNVDYILERDIRLGDEVFVEKAGEIIPQVIGVDPSKRKDYPPLEIP
                     KLCPSCQSPLVHLEEEVALRCVNATCPAKLLRLLEHFVSRDAMEIQGLGERILSSLMD
                     GGYLTSPEDLYYLKDHREDLIVLPGFGEKSIDGILEQIEKSRTKTLDRLIFALGIQHI
                     GRVAARELALYYRDLKDLRAAKEEELLSISGIGPTLVKSLKEYFENEDNQVFLDHLEN
                     MGLGLPLKEEKAQGNFQDLRFVVTGRFELPRRKIEEALRQAGAKVSTSVSKNTDYLLL
                     GENPGSKYDKAQELEISVLSLEEVKNMGLEIE"
     gene            complement(5769..7886)
                     /locus_tag="VFD08_04710"
     CDS             complement(5769..7886)
                     /locus_tag="VFD08_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZK10351.1"
                     /translation="MNIEGLNPEQKKAILTEKGPLLIMAGAGSGKTRTLITRIAHLIE
                     RGVPAWKILAITFTNKAAREMRERLDELVEEETSSLWVGTFHGISLRILRRYSETVGY
                     QSDFVIYDTTDQRTLISQCLKELDMDTKLYPPKMVRGLIGQAKSQMVRLSQYKEYLDE
                     DLWEVFSLYEKKLREYQAMDFDNILFRLVQLFEENEEIRQSYEQRFHEVLVDEYQDTN
                     KIQYHLIHLLTRSKKNLVVVGDIDQSIYGWRGADIRNIVDFKKDFPGAKVITLEQNYR
                     STKRILAAANAVIENNPSRPKKNLWTDLETGDKIFLFNAKSDQEEADYVLTSILADLE
                     AGVSASEIAILYRTHAQSRLFEERLRRADIAYQIYGGQQFFERREIKDALAYLRVLVN
                     PRDEISLLRAIALPRRGLGEVSLSKISSYASQEGLGFYEGALKAVEEGVFTKRIAGSL
                     GEFLQLFEEAKKALEEGDPYLVSLELLEKSGYLPMLKNSKDIEDKGRLENVEELLSDI
                     ASFYERGEGGLEEYLLNVSLLTNMDEETSENPVMLMSLHSAKGLEFGHVYLVGMDEDL
                     FPSYMSKESDEEMEEERRLCYVAITRAMKKLVLTRADTRFRFGEPQWMQASRFLGEIP
                     QELLHIEGKSKGNAFQRTIPFGKEKKGGKGDFMAGDKVSHKIFGQGVVVGIQKDKKKV
                     QVVFTKVGVKELHLDYAPLRKEA"
     gene            complement(7883..9391)
                     /locus_tag="VFD08_04715"
     CDS             complement(7883..9391)
                     /locus_tag="VFD08_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10352.1"
                     /translation="MFSNDLLKRYLKYFILIASVIVVVLGFLWSNFLYTRHQISLETL
                     SEGMAESMEEEVKQKAQILRQRAEVIHEQESEDLIIDLKNQGATLRAALYDLYFEYEL
                     EGEKKEEEIRKAIEKIQSQQIYDVYFVSDNLIIGQIPENIFSLEEGINNQKSHLIYCD
                     KSKSLNMEFYISVDQQSFIKKRMEENFKAFALWDEGMHILTKEGEVILPIQETVLAKD
                     DFFYQEESSQTGFVFGYLIPHSQLQDRIDERRDLYSSFLDSHIWEIIGFLFIFIITSF
                     VIYRMIISRLEDYYFALNEEILDAYRNKDTLSDNPKFQHFALGDSFDSILRESQINKE
                     KDRQKIISLEEKLKKDKIEKLLLERKIRKLSELPYTKAILHNYTTESFSPKEIISKIH
                     SKIDPQARMLIKGSNEFINNDKGLFKALVEEVFQLTQEENRSYAIEVFRDGGQILLYF
                     TIYGLGLLEDEKMQELKNRAKLLEGVFLRHQTEEQTLHLVLSLNDIHRNEEK"
     gene            complement(9431..9838)
                     /locus_tag="VFD08_04720"
     CDS             complement(9431..9838)
                     /locus_tag="VFD08_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK10353.1"
                     /translation="MKNIAFYLTPKINVVFEYKDASMRQLFEKMEHHRYTSIPILNEK
                     GHYEGTITEGDILRKISKKDTISFKDMENLYVKDIKRSKLYSPVSIDADFKDLLNVIA
                     HQNFVPVVDDQGVFIGIVTRSAILSSFEKLLAE"
     gene            complement(9941..11158)
                     /gene="purD"
                     /locus_tag="VFD08_04725"
     CDS             complement(9941..11158)
                     /gene="purD"
                     /locus_tag="VFD08_04725"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZK10354.1"
                     /translation="MRILLVGSGAREHALLYSMKKDYPTYEYYALPGNPGMEIPSISL
                     QEAMEKNWDLVLIGPEEPLVKGMADVFREKGIPVFGPGAEAAALEGSKIFSKNFMRKY
                     AIPTAKYKVARSYNEALNILMKFSELPVIKGDGLAGGKGVFLPRSVNEAQSIIEALMV
                     EDLLGSSGSKLVFEERLEGKEISFFYLVNEKGYRYLGCARDHKRAHDGGEGLNTGGMG
                     TFSPVPDMGEKDLIEIDEIMDKTFQGMMAEEMDYRGVLFIGCMRTKEGLKVLEYNVRF
                     GDPETQVLMARLKSDFLELALAVAKNEELPEVVLTDQVALCVVLASQGYPGEILKDQP
                     IQIGNLKEDLLLFSGGTSLIDGVLVNSSGRVYSLVSLGENLPEARKKVYDNIGQIQFK
                     GVWYRKDIGVVSN"
     gene            complement(11169..12659)
                     /gene="purH"
                     /locus_tag="VFD08_04730"
     CDS             complement(11169..12659)
                     /gene="purH"
                     /locus_tag="VFD08_04730"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZK10355.1"
                     /translation="MRALISVYDKNGLEKLLLSLLKKGYELISTGGTYDFIKKLGYDV
                     TRVEEITGAPEILGGRVKTLHPKIHGGILACADNEAHIKEIEKENIKPINLVICNLYP
                     FFEKVKEPLTFEEKIEFIDIGGPSMLRAAAKNHRFVTVLSKPEQYDEFLARLNKDDLD
                     YDYKKKLAGEVFALTASYDSAIAAFISKEDYPEHLVLSYEKKGELRYGENPHQRAAFY
                     ADSSQGGFLSDLEVLWGKELSYNNYRDMEVAWELSQEFEDICAVAVKHNTPCGVALGQ
                     TSAEAYQKAFDCDRVSVFGGIVALNNEVDKKTAAAMGKTFLEIVMAPSFTPEALEELK
                     KKKNLRIVLVSPAAEVDKNFLSLGGGLLLQDKDKEFIKELRCVTKRQPSLEEEEEMLF
                     AMKVVKYVKSNAIVVSENKAARGIGGGSVNRIDAAKYALEHAPGAKVLASDAFFPFSD
                     VIELAAEKGIKAIIQPGGSIRDKDSIEVCDKKGITMLFTGMRHFKH"
     gene            complement(12647..13198)
                     /locus_tag="VFD08_04735"
     CDS             complement(12647..13198)
                     /locus_tag="VFD08_04735"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZK10356.1"
                     /translation="MKIVVFASGSGSTFSYLAQHQKHYSIEALFSNKKTAGVVEKAQK
                     LGIPLIYVDKDNRWKDKLKKLAPDYILLAGYLKIIPKDVVEGYPERILNLHPSLLPKF
                     GGKSYYGDRVHRAVLEAGEKETGSTVHLVDENIDSGKILGQVKVPVYEGDTVETLAAR
                     VQEAEKPLYLKEFLRYGGIKCEH"
     gene            complement(13195..14193)
                     /gene="purM"
                     /locus_tag="VFD08_04740"
     CDS             complement(13195..14193)
                     /gene="purM"
                     /locus_tag="VFD08_04740"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZK10357.1"
                     /translation="MKITYKDAGVDVDRGQDAVKEMKAYVEASQGPEVLTELGSFAGL
                     FLLPSGLQEPVLVSGTDGVGTKILMAKATGFSENIGQDLVAMCVNDILCHGARPLFFL
                     DYLASAYLDPKEVTKVVASIARACKEVGCALIGGETAEMPGMYQPGDMDMAGFCVGIQ
                     ERKKLITGEKIKEGDCLIGLASSGFHSNGYSLLRKIYLEHYPQYLAEYGEELCQPTTL
                     YVNPVLALLEKVEVLGISHITGGGFYENIPRALPGGLSFDVDRSLWQRSPLIELTAKL
                     TGLEKEELYSTFNMGIGMVLIVRQEDKKESLELLEEKGLQAWDIGKVISADKGIIR"
     gene            complement(14237..15649)
                     /gene="purF"
                     /locus_tag="VFD08_04745"
     CDS             complement(14237..15649)
                     /gene="purF"
                     /locus_tag="VFD08_04745"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZK10358.1"
                     /translation="MNNFLDKLHEECGIVGIYSQEYQHLGPKICDALISLQHRGQESA
                     GISVCHEGVMMHYKNMGLVRDVFNEENLRPFNGNVGIGHCRYSTLGSAERRNAQPLVA
                     EFKAGTMALAHNGNLINAVQIRERLIDEGFSFTSTSDSEIVIKLIAIQYKGNPLEAIK
                     KALAQIKGGYALVLIVEGKLYGIRDPYGLRPLVLGQTDKGDYVLASETVALDLLKAKF
                     VREVDKGEIIEIDDTGYHSHYFDQTKPMSLCGFEFVYFSRPDSDLAGKNVYQTRIELG
                     RILARQDKEKYDLVAAVPESGVSSAIGYAMEADTVYSSVFIKNKYLGRTFTDPSQEGR
                     ERMLRLKLSILRSNIQGKRIVIVDDSIVRGTTLRSIIIDLKESGAKEVHVRISSPPVG
                     YSCFFGIDTPSKKSLIAANMSVEEIEEYIGADSLRFLTPEEFIEGVGLPENTLCKACF
                     TGKYPMEVPKAHNKYVFEKY"
     gene            complement(15642..16130)
                     /gene="purE"
                     /locus_tag="VFD08_04750"
     CDS             complement(15642..16130)
                     /gene="purE"
                     /locus_tag="VFD08_04750"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZK10359.1"
                     /translation="MKVAIVMGSKSDLPVVEKAALILEEFAVEHKMRILSAHRTLDAT
                     LDFVRGAKGQGYGVIIAAAGKAAHLPGVIAAATDLPVIGIPIKTSMMGGLDSVLSILQ
                     MPGGIPVATVGVNAAENAALLALRILALSDEKIALSYRTYVQKMEEKVLEDDRELETK
                     NE"
     gene            complement(16127..19684)
                     /locus_tag="VFD08_04755"
     CDS             complement(16127..19684)
                     /locus_tag="VFD08_04755"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HZK10360.1"
                     /translation="MNKIYVEKKPEFRSKNLQLQREIEALYGFELEDLRIINLYYRPQ
                     EFSEEIINKIFFDSALDLKSYSFEPPTGYRHLRTEFLPGQFDQRSYWASKCLELLEEE
                     EEIYFSHIYLFKGLPDEKWEEVKDFFINPVESFERDLEDFSLAQPRRDKDITYQSMED
                     IFTLDGEDFCLKHNLAMTGADLAVIEDYFKEEGRAPTYSELRLLDTYWSDHCRHTTFN
                     TELCQIDFSKNNLPLAEEVFAYYEKNRSLYTKKPPSLMELAIFSMRKEKAQGNLEDLE
                     ESEEINACSIERNFAGEDYLIMFKNETHNHPTEIEPFGGAATCLGGAIRDPLSGRSFV
                     YQAMRVSGARDPKDPRVLPGKLPQQIISTRAAQGYSSYGNQIGICTGHVKEYYHPGFE
                     AKRLEAGFVIGSAKRSHVVRGIPKKGDLLLLLGGATGRDGIGGASGSSKSHQEDVATG
                     AASEVQKGNAPEERKLQRFFRMEDIGPKIVRCNDLGAGGISVAFGEIAPGLEIEIEDV
                     PTKYKEIYPWEILLSESQERMALVIEPRDLELFKKRAKEEDLSLTVIGKITDKERFIV
                     SHGGKVLVDLHRDFIEAAGAPQRADVEVEPIKLDLYPYKKELDSKELLEASQQGLVEM
                     FDSSVGGLNVVSPYGGKRRKSFVDGMVSKIPGVKTDDVTIASQGFDPYLAQWSPFHGA
                     EMAVMQSISKNLVLGGDLKGLRLSFQEYFEALGKDPKKWAKPFMALLGANRVLHHFKL
                     ASIGGKDSMSGSFFWEGGEIHVPPTLISFCVNTGKLAHVRTSEFKKAGSELYLYEIPL
                     REDYCIDLEGAKKALEGYISDREKILSASVVTLSLVHTAENAAFGNHLGCQLLEDGHP
                     LGSILVEVEAGSKLTGARLVGRTIEEAELHLKGEKRCLDASLKKKEAHLDPIFELGMT
                     LAPEKAMEKKSFLQEGKKERVKVFIPIFPGTNSEDEFGNKFEEEGAEVDRWVFSTSDM
                     DSSLTTLAKKIKECDILGLAGGFSAADEPEGSAKFIATVFRTKKVKDAFHELVDQKGL
                     VIGICNGFQALVKLGVFYNGRIEEADKVTMGLTYNDLGRHQSALVRIRCLNQNSPWLS
                     NVEEQNYWCPISHGEGRFFMTEEDRQLLVSRKQIATVYVDNPNGSMMDIEGLLSPDGR
                     IFGKMGHNERLGPDLFVNIDHKKDMDIFSAGLEHFRRAK"
     gene            19770..20657
                     /locus_tag="VFD08_04760"
     CDS             19770..20657
                     /locus_tag="VFD08_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZK10361.1"
                     /translation="MQNLESYRSFYMAARFENFTRAAEELFVTQSSVSQSVKKLETSL
                     GIDLFLRRGRRVQLTEEGKVLYKELDKVFKTLEDAERLMMEYKSVEKGSLSLAASDTI
                     CRHYLLPVLKSYKEEFPHIHINIINQPSPIVAELVRTGQVDLGFVNGQSSDYSDLVPL
                     ALLNLKEQFFCAEKSPIPKRTLSLEELLDYPQVCLKQQTSTRQLLDDLFRKERLKFQP
                     EVEVISIDLLIDLIRVGFGIGFSHSHLIKKEGLRLIKVDQDLPQRKLLLLTNPRISPS
                     KAGGLFLDLCHSFFFCHSA"
     gene            complement(20654..>20903)
                     /locus_tag="VFD08_04765"
     CDS             complement(20654..>20903)
                     /locus_tag="VFD08_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HZK10362.1"
                     /translation="TISIDYLPEGDYILKETRSLRGYIPCEEQKINFENYGVNSPLLI
                     ELVNKRELPKTGEKSSHGLVFLSIALAAAGFLIQKRFN"
BASE COUNT         5731 a         4894 c         3639 g         6639 t
ORIGIN      
        1 gtacataata agaattcttc aagtgtttgg ctctagattt acctgcatct tcttcatcct
       61 caaaaagact gctggtggat tcatatattt gcttaatcct atttgcatta tatataatct
      121 ctaaaacacc ctttatttcc cccttaatta taatcggtat atttgttaca atcgaagtaa
      181 cttttcttcc tttaaaatta atataagtct gtaggtcatc atatatgggt tttcccgttt
      241 ctatacactg tagaatcgtg ctagtttgag gatcagaaga gggaaataca tctaaaacat
      301 gcttcccaag catttctccc gtttctagtc cttccatttc ctctagggct cgattgtaat
      361 agagaaagcg accatctaca cctattaccg cgattcctag ttgagtgtgg tccgaaatca
      421 gttcaaataa ttgttttcca tactcttcaa aaatcaccca tacctcctca tggtcattac
      481 ttactcagct atttattatt actttgtgag tatttgcatc ttttcatggg tattcttacc
      541 gcttccgtta aaattataat taaatgcaaa aagcctataa ccaagatggc tataagcttt
      601 ttgatggcga ggtatagagg attcgaaccc ccggccccta gatccgaagt ctagtgctct
      661 atccagctga gctaatacct cctactgttt ttaattgtac aactttatag cttaaagtgc
      721 aagagaatat actatctttt atcctactca ttaatcatgt taaccaataa aaaaagaaac
      781 atcgtttctt tattagcttg gcgcgcatag agggattcga acccccggcc ccctccttag
      841 gagggaggtg ctctatcctg ctgagctata tgcgcaatat atatggggcg gctaaaggga
      901 ttcgaaccct cgaatgcagg agccacaatc ctgtgtctta acctcttgac gatagccgcc
      961 atatttaaaa actggtcggg gtggcaggat ttgaacccgc ggccccctgg tcccaaacca
     1021 ggtgcgctac caaactgcgc tacaccccga tgcagcaact ctaataatac agagaaaagt
     1081 tcaaattgtc aactacaaaa gtgatggttt ttgttacatt tttttactat acctctttta
     1141 ctatcacttc taccccagct tcttggattt gcaaaataag ataggttcct cttttgccag
     1201 gaggtggaag agacacactg cctggattca aaaaaagtat ctggtcaatt gtccttttta
     1261 ggggctggtg tgtatgacca aatagaacaa tatctgccct ttcttccttg gccctgtaga
     1321 aaagattgtg caaatctctt tttacccctt ctttatggcc atggctcatc ataatgcgtt
     1381 gcccctttag ttcaaacacc ctccttaaag gagctgcact cataccatca caattgccac
     1441 gcacactcag gccttcttta agatcttttc catagtctcc taggtgcaga cttagatcgg
     1501 gcttttcttt ttttacaatt tcatcaactg tttttttgtc tccatgacta tcagagataa
     1561 ctaatacctt catttttgag tttttcctcc aaacgcctaa gggcgtccca tctatgagaa
     1621 atactatgtt ttttctccat agacaattct ccaaagcttt tatttaaaga aggataataa
     1681 aaaatgctat caaaaccaaa gccgagctct cctctttctt ctagggcaat ttcaccaaaa
     1741 caaagtccca gctgggtaaa ttccttgtca tccacaagaa aggcaatctc acaggcaaaa
     1801 aaagcattcc tgttttctag gcccttcatt tcttctagca ttttttttcg tttttcttca
     1861 aaggaaatat caccaccata gcgagctgaa tgaattcccg gtcctccccc tagggcctca
     1921 acaattatcc ctgaatcatc actaagcaca ggaagatccg taaattttct tacttctcta
     1981 gccttaatta aagcattctg acgaaagctt ttaccatttt cttcgatctc tctattaaaa
     2041 cctaattctg ccaggctaat gaacttcacc ttccaatttc gcaagatata acgaatttcc
     2101 tcaattttac cagggttctg gctggccact actatagttt tcataggact gatcctgctt
     2161 tcttatgatt tcttctatcc cctctgcagc caaatctagc atagtatgaa gttgtgctct
     2221 agagtaagta gatctttctg ctgtaccttg gacttcaata aaatttccct gctcatccat
     2281 aactacattt aggtctactt ctgccctact atcttgttga aaacttaagt ctgtaattac
     2341 ctgagcatca acaataccca cgctcacagc agcaagcttt ctttgaaggg gattactgtg
     2401 taagtatcct ttttgcatca aatcatccat ggcaaggcga agagcaagat aggctcctga
     2461 aatagctgct gttctagtgc ccccatcagc ctgcaaaacg tcacaatcaa catagagggt
     2521 catggggcct agaagggaga aatctagggc caggcgcaag cttcttccaa ttagcctttg
     2581 tatttcaatt gttcttccat caatctcacc agaaatagtt cgtctttgtt ttcttgtaaa
     2641 agttgaaccc gggagcatag agtactctgc tgtcaaccag ccttctccat tagggcggaa
     2701 cttgggaacg ccttctactg catagactgt gcaaaggacc tttgtctgac caaaaacggc
     2761 aagtacactt ccatgcgcat gttttgtata accacaaata aattcaatcc ttctcattga
     2821 ttaccccatt accttataca gttcatctcc ggccttcatc tctacctcaa catcctgatc
     2881 aaaacgcaaa atctttcttt ggtctgtcct aaattcccca atatgagaag cttctattcc
     2941 ctttgctgat agttcttgca aaagtccctc cacttcctct ttaggaagtg tcatcaaaag
     3001 agagccagag ctaatcaact gaaagggatc aatatcataa aagtctgaga ctttatgggt
     3061 gacatcatga atgggaacct ttttactatc aacaaaaaat ccaagattac tctcaaaaca
     3121 catctcacta agggccccca caaggccacc ttcagtgata tcatgcataa aatttacccc
     3181 atgatccaaa ccaattcgtg cctccttggc aatagaaagt tgctgcccca tttgctgggc
     3241 gtcttcgatt tcttcttttg taagatgggc tcttatttct tctttttctg caacaataat
     3301 agataccccc tcaagcccaa gagattttgt cataacaatg gcatcatttt cttggattcc
     3361 tttaaggggt ctttccactc gacccagggc tgtaagagaa agaacgactc gatttactgc
     3421 tgttgtaact tctgtgtggc cacccagaat atccacattt agcttagtgg ccatggtctg
     3481 agcgtcttcc ataatctctg caatttcttc ttttttaata tggggaggca aaagcatggt
     3541 aatcataaca gccaagggct caccaccccc agccgagata tcattgcaag caatcaaaag
     3601 ggctaaggat ccaatgtttt ttcccgctcc cgtaatagga tcactggcca ctgccattaa
     3661 tttttcgcca aactgtaaat atgccacatc taagcccacg ccaggtccca tctttgttgt
     3721 tgaacgatgg ttttttattt ttgagaggac cagctgattt agatcctctg tagcaagttt
     3781 tcctatatac attaaatacc tctactctat ttctagtccc atatttttaa cttcctctaa
     3841 actaagaaca cttatctcta attcttgtgc cttatcatac ttactcccgg gattttcccc
     3901 taaaagcaga taatcggtat tttttgatac agaagtagag acctttgccc ctgcctgtct
     3961 aagggcttct tcaatttttc ttcggggcag ctcaaatctt cctgtaacaa caaaacgcag
     4021 gtcctgaaaa tttccctggg ctttttcttc ttttaaagga agacccagac ccatattctc
     4081 cagatggtct agaaatactt ggttatcttc attttcaaaa tactctttta atgacttgac
     4141 cagggtaggg ccaattccag aaatacttag cagttcctct tccttggcgg ctcttaagtc
     4201 ttttaaatcc ctgtaataaa gagccaattc tcttgctgcc actcgtccaa tatgctggat
     4261 accaagagca aaaataagac gatctagggt cttcgtgcga cttttttcga tctgctcaag
     4321 tattccatct atagattttt ccccaaatcc tggtaaaaca atcaagtctt cccggtggtc
     4381 ttttaaataa tataaatcct cagggctggt taagtagccc ccatccataa gactagataa
     4441 aattcgttct cccagccctt gaatttccat agcgtctctt gaaacaaaat gctctaataa
     4501 acgcagaagt ttggctggac aggttgcatt aacgcaccgc agggccacct cctcttcaag
     4561 gtggaccaag gggctctggc aagatggaca tagtttagga atctctagcg gaggataatc
     4621 ctttcttttg gatggatcta ccccaatgac ctggggaata atttctcctg ctttttcaac
     4681 aaaaacctca tcaccaaggc gaatgtctct ttcaaggata taatctacat tatgcaaact
     4741 agctctttgc accttggtcc cagctagttg taccggggcc aggaccgcgg ttggagtcac
     4801 aacacctgta cgaccaacag tccaaaccac atcaagtaat ttggtctgag acctttctgg
     4861 agcaaattta taggcaatgg cccagcgggg ggccttgctt gtttctccca ggcttctttg
     4921 aagggcttct tcatttacct tgattactag gccatctact tcgaaatctt ttttgttttt
     4981 ctcttcttca tagataggga ttctatcgat aattgccctc attttttcaa aaacttctac
     5041 cgagattacc ctaaagccta gagaacgtaa atagtctagt tttttatcat gatggctaaa
     5101 actaggtccc tctaataggt caaaaatcca aatagaaagt cctcgcttgg ccattaattt
     5161 gctgttgagc tggcgaatac ttcccgcagc agcatttctt ggattggcaa aaggtgcaag
     5221 tcctgcctct tcttgttgtt tatttagttg gataaaattc ttcttgggca gataaacctc
     5281 acctctaaca ataagatcaa caggctcact aagctccagt ggaatggccc ctatggttct
     5341 taagttttgc gttacatcct ctccttgaag accatccccc cttgtggcac caagcacaaa
     5401 gcgtccctta tggtaatgaa gaacaacact aagtccatcg actttttctt ctagtacata
     5461 gctgggcctt ggctctatat tctctacacg gcgagaaaat tttatcaaat cttcttcatt
     5521 ataggaatta tccagactcc ccagaaattt ggggtggggt acttttacaa aaccagaaag
     5581 tagctctccc cccactcttt gagtaggaga agaaggatcc aagagctctg gatgctcttt
     5641 ttccaaggct tccacctcct tataaagaag gtcataattc tgatcactaa tttctggact
     5701 atcaagaaca tagtatagat gactatgttt ttttaactgc tctagtagat tttggtattt
     5761 ttcttttatc atgcttcttt cctcaagggg gcataatcaa gatgaagctc ctttacaccc
     5821 acctttgtaa agaccacttg tactttcttt ttatcttttt gaattcccac tacaacaccc
     5881 tgaccaaaaa ttttgtggct gaccttgtct cctgccataa aatctccctt tccccccttt
     5941 ttttctttgc caaagggaat ggttctttga aaggcattgc ccttactttt tccctcaata
     6001 tgaagaagtt cctgagggat ttctcctaga aatcgagagg cctgcatcca ttgaggctcc
     6061 ccaaagcgaa atcttgtatc tgcccgggta aggactagtt ttttcatggc tcttgtaatg
     6121 gctacatagc aaaggcgtct ttcttcttcc atttcttcat cgctttcctt gctcatatag
     6181 gagggaaaaa gatcctcatc catccccacc agatatacat gaccaaactc caagccctta
     6241 gcactatgaa ggctcataag cattacaggg ttttcagatg tttcttcatc catattggtt
     6301 aaaagagaga catttaacag gtactcctct aagcctccct ctcctctttc gtaaaaagaa
     6361 gcaatatcac taagcagctc ctccacgttt tcaagtcttc ccttatcttc aatatcctta
     6421 ctgtttttga gcatgggtaa atagcctgac ttttctaaaa gttcaagact cactaggtag
     6481 gggtcccctt cttccagtgc tttttttgct tcttcaaata attgaaggaa ctcccctaaa
     6541 gaccctgcaa ttctcttggt gaaaactcct tcttctactg cctttagagc tccctcataa
     6601 aaaccaagac cttcttgact ggcataggaa gaaatcttac ttaagcttac ctcccctagg
     6661 ccccttcgag gaagggcaat tgctcgaagc agggagatct catcccttgg attgaccaag
     6721 accctcagat aagctaaggc gtctttgatc tctctgcgct caaagaattg ctggccacca
     6781 taaatttggt aagcaatatc tgcccttcta agcctttcct caaaaagtct actctgggca
     6841 tgggttcggt aaagaatggc aatttcactt gctgatactc cagcctctaa atctgcaaga
     6901 atacttgtta gtacataatc tgcttcttct tggtcacttt ttgcattaaa taaaaaaatc
     6961 ttatcgcctg tttccaaatc tgtccaaaga tttttctttg ggcgagaggg attgttttct
     7021 atcacagcat tggctgcagc cagaatacgc ttagtactcc ggtagttctg ctctagggta
     7081 attaccttgg ccccagggaa atctttctta aaatcgacta tatttcttat atcagcacca
     7141 cgccagccat aaatagattg atctatatct cctacaacaa caaggttttt tttggaccta
     7201 gttagaagat gaatcaagtg gtactggatt ttgtttgtat cttgatactc atctaccaaa
     7261 acttcatgaa aacgttgctc atagctttgg cggatctctt cattttcttc aaatagttga
     7321 accaggcgga ataaaatatt gtcaaaatcc atggcctggt attctcttag ctttttctca
     7381 taaagggaaa aaacctccca gagatcttca tctagatatt ccttatactg actcaggcgg
     7441 accatttggc tttttgcctg tcctataagc ccgcgaacca tctttggtgg atagagcttg
     7501 gtatccatat cgagttcttt taaacactgg ctaatcaggg ttctttggtc ggttgtatca
     7561 tatataacaa aatcactttg atagccaacg gtttcagaat atcttcttag gattcgaagt
     7621 gatatcccgt ggaaggtccc tacccataaa gaggaagttt cttcctcaac aagttcatcc
     7681 aggcgttctc tcatctctcg tgccgcttta ttggtaaagg taatagccag gattttccat
     7741 gctggcacac cacgctcaat aagatgagca attcttgtaa ttaaggtacg ggttttcccc
     7801 gagcctgctc ccgccataat gagaaggggg cctttttctg taagaattgc ttttttttgc
     7861 tctggattta gtccttctat attcattttt cctcatttct atgtatatca tttaaagata
     7921 gaactagatg aagtgtttgc tcctcagttt gatgtcgcaa aaaaacaccc tcaaggagtt
     7981 ttgccctatt cttaagttcc tgcatctttt catcttcaag taaacctaag ccataaatag
     8041 taaaatacaa aagtatttgg cctccatctc taaaaacttc aatggcataa gaacggttct
     8101 cttcttgagt gagttgaaaa acctcttcaa ctagggcttt aaacaagccc ttatcattat
     8161 ttataaattc attgctccct ttaataagca tccttgcctg ggggtctatt ttactatgaa
     8221 tcttagaaat aatttccttt ggcgaaaaac tttccgtagt gtaattatga aggatggctt
     8281 ttgtataggg taattctgag agctttctaa tttttctttc taagagaagc ttttcaatct
     8341 tatctttttt tagtttttct tccaaactaa taatcttttg cctatctttt tccttattga
     8401 tttgagactc tcttaagata gagtcaaagg agtctccaag agcaaagtgc tggaactttg
     8461 gattatcact aagagtgtct ttatttctat aagcatcaag aatttcttcg tttaaagcaa
     8521 aatagtaatc ttctaaacgg ctaataatca tccgatatat gacaaaactg gtaataataa
     8581 atataaataa gaaccctata atttcccata tatggctatc taaaaaactc gaataaagat
     8641 ctcttctctc atcaatacgg tcctgaagtt gggagtgggg aataagatag ccaaaaacaa
     8701 atcccgtttg actactctct tcttggtaaa aaaaatcatc ttttgcaagc acagtttcct
     8761 ggatcggtag aattacctcc ccttctttgg ttagtatgtg cattccctca tcccataggg
     8821 caaatgcttt aaagttttct tccatgcgct ttttgataaa actctgctgg tctactgaaa
     8881 tataaaactc catatttagg ctcttggatt tatcacaata aatcaaatgc gatttttgat
     8941 tatttattcc ttcttctaaa gagaatatat tctctggaat ctgccctata attagattat
     9001 ctgagacaaa atacacatca tatatttgct gcgactgaat cttttcaatg gcttttctta
     9061 tttcttcttc ttttttttct ccctctagct cgtattcaaa gtacaaatca taaagggctg
     9121 cccttagagt ggctccttga ttctttaggt ctataattag atcttcactc tcttgctcat
     9181 ggattacttc tgctctttga cgaagaattt gtgccttttg tttgacttct tcctccatag
     9241 attctgccat accttctgag agggtttcaa gacttatttg gtggcgagta tataaaaagt
     9301 tggaccacaa aaaccccagt accacaacga ttactgaggc tattagaata aaatacttta
     9361 gatatctttt taaaaggtca ttgctaaaca aagcaggccc cctttacaaa ggcaaaatct
     9421 attcccaaag tcattctgct aatagttttt caaagctcga taaaatagca cttcgggtta
     9481 ctattcctat aaacacccct tgatcatcta caacagggac aaaattctga tgggcaatca
     9541 catttagtaa atctttaaaa tcggcatcaa tggatacagg agaatacaat ttgcttcttt
     9601 ttatatcctt tacatagagg ttttccatat ctttaaagga aattgtgtct tttttgctga
     9661 tttttcgcag aatgtcgccc tcggtaatgg ttccttcata atggcctttt tcatttagta
     9721 tggggatgga tgtatagcgg tgatgctcca ttttttcaaa aagttgccgc atactagcgt
     9781 ctttatactc aaaaacaaca tttattttag gggttaaata aaaagcaata tttttcattc
     9841 ctactccttg tccttattga ctggggaagt gtgtatcttc tcatcaaaat accagcactt
     9901 tgattataaa tgaaagtgcc ccaaagttac aaggagtaaa ctagttgcta acaactccta
     9961 tgtctttacg gtaccagaca cctttaaact gtatttgacc tatattatca tagacttttt
    10021 tacgggcctc tggcaggttt tctcctaggg ataccaagga atacacccga ccactgctat
    10081 ttactagtac cccatcaata aggcttgtac caccggaaaa caaaagaaga tcttccttca
    10141 agtttccaat ttgaatgggc tgatctttga gaatttcgcc tggataacct tgggaagcaa
    10201 ggacaacaca aagagctact tgatctgtta aaaccacctc aggaagttct tcattcttag
    10261 ccactgctaa ggcaagttct aagaaatcgc ttttaagccg ggccattaaa acttgggtct
    10321 caggatcgcc aaaacgcaca ttgtattcta agaccttaag gccctcctta gtgcgcatgc
    10381 agcctataaa taaaaccccg cgataatcca tttcctcggc catcattccc tgaaaggttt
    10441 tatccataat ttcatcaatc tcaattaagt ctttttctcc catatcaggt acaggtgaaa
    10501 aggttcccat tccaccggta tttaggcctt ctcctccatc atgagccctc ttatgatctc
    10561 tggcacaacc aagatagcga tagccttttt cattgacaag atagaaaaag ctaatttctt
    10621 tgccttctaa tctttcttca aaaaccaatt tagatccact acttcctagt agatcctcca
    10681 ccattaaggc ctctataata gactgggcct cattgacact tcttggaaga aaaactccct
    10741 ttcctcccgc caagccatct ccttttataa caggaagttc actgaatttc attaggatat
    10801 ttagggcttc attataagaa cgagccacct tgtatttggc tgttggaatg gcatattttc
    10861 tcataaagtt tttactaaat atcttgcttc cctccaaggc cgccgcttca gctcctggac
    10921 caaaaacagg gattcctttt tctctaaata catctgccat gcccttaact aggggctctt
    10981 ctggaccaat taggaccagg tcccagtttt tttccatggc ttcttgtaaa cttatagaag
    11041 gtatctccat accaggattt cccggcaaag cataatattc ataagtggga tagtcttttt
    11101 tcatggaata gagcaaggca tgttctctgg ctccagagcc aacaagtaat attctcatat
    11161 aatcctccct aatgtttaaa gtgacgcatc ccagtaaaga gcattgtaat tcctttttta
    11221 tcacaaacct cgatagaatc cttatctcta atggatcctc cgggctggat gatagccttt
    11281 attccttttt ctgctgcaag ctctattaca tctgaaaagg gaaagaaggc atctgaggca
    11341 agcaccttgg ctcccggggc atgctcaagg gcatattttg ctgcatcaat tcggttgaca
    11401 ctgcctccac caattccccg agctgcctta ttttcactta ctacaattgc attgctttta
    11461 acgtatttaa ccaccttcat ggcaaaaagc atttcctctt cttcctctag agaaggttgg
    11521 cgtttggtga cacagcggag ttctttaata aactccttat ccttgtcttg taggagaaga
    11581 cctccgccta gacttaaaaa gtttttgtcc acctctgctg ccggggaaac aagaacaata
    11641 cgaaggtttt tcttcttttt tagttcttct agcgcctctg gagtaaaact tggtgccata
    11701 acaatttcta aaaaggtctt gcccatagca gcagctgttt tcttatcaac ctcattatta
    11761 agagcaacaa tccccccaaa cacagagacc cgatcacaat caaaggcttt ttggtaggct
    11821 tctgcgctcg tttggcctag ggccactcca caaggagtat tgtgttttac tgctacagca
    11881 catatatctt caaattcctg agaaagctcc caagcaactt ccatatcacg atagttatta
    11941 taagatagct ccttccccca aagaacttca agatcactta aaaatcctcc ctgggaacta
    12001 tcagcataaa aggctgctct ttgatggggg ttttccccat agcggagttc tccttttttt
    12061 tcatagctta gaactaagtg ctcgggataa tcttccttgg aaataaaggc cgcaatcgca
    12121 ctatcatagc ttgccgtaag ggcaaaaact tcccccgcaa gtttcttttt atagtcgtag
    12181 tcaagatcat ccttgtttaa acgcgctaaa aactcatcat attgctcggg ctttgaaaga
    12241 accgttacaa agcgatggtt ctttgccgct gctcttagca tactagggcc gccaatatct
    12301 ataaattcaa ttttttcctc aaaagttaag ggttctttta ctttctcaaa aaaaggatat
    12361 agattgcaga taactaagtt aatgggcttt atattttctt tttcaatttc ttttatgtga
    12421 gcttcattat cagcacaagc cagaattcct ccatgaattt ttgggtgtag ggttttaacc
    12481 ctgccgccca aaatttctgg tgcaccggta atttcttcta ctctagttac atcataacct
    12541 agtttcttga taaagtcgta ggtaccgccc gtactaatta gttcatatcc tttcttaagc
    12601 aggctcaaaa gaagtttttc tagtccgttt ttatcgtaca cactaattaa tgctcgcatt
    12661 ttatccctcc ataacgtagg aattccttta agtatagggg tttttccgct tcttgaaccc
    12721 tggctgccaa ggtctcaact gtatcacctt catagacagg aacctttacc tgccctagta
    12781 ttttaccact atcaatattt tcatctacca aatgcacggt ggaacctgtt tctttttctc
    12841 cagcttctaa tacagcccta tggacccggt caccataata ggattttccc ccaaacttag
    12901 gtagcaggga agggtgtaga ttaaggattc tttcagggta gccctctaca acatctttag
    12961 gtataatttt caaataaccc gctaataaaa tatagtcggg ggccagtttt tttagcttgt
    13021 ctttccacct attgtcttta tccacataaa taagaggaat tccaagtttt tgggcttttt
    13081 caaccactcc tgctgtcttt ttattagaaa acaaggcctc aatagagtag tgtttttgat
    13141 gctgggccag ataagaaaaa gtagacccac ttcccgaggc aaagaccact atcttcatcg
    13201 aatgattcct ttatcagcag aaataacttt tccaatatcc caggcttgaa gacctttttc
    13261 ttctaataac tccaggcttt cttttttgtc ctcttgtcta acaataagga ccataccaat
    13321 tcccatatta aaagtactat agagctcttc tttttccagt ccagttaact tggctgtaag
    13381 ttctattagg gggctcctct gccacaggct tctatcaaca tcaaaactca atcccccagg
    13441 cagggccctg ggaatgtttt cgtagaagcc tcctcccgta atgtgactta tacccaaaac
    13501 ctctaccttt tctaaaagag ccaaaacagg atttacataa agggtggttg gttggcagag
    13561 ttcttcgcca tactcagcta aatactgggg ataatgttct aggtatatct ttcttaggag
    13621 tgaatatcca ttggagtgaa aaccgctaga agctaggcca ataagacaat ctccttcttt
    13681 tatcttttct cctgtaatta acttttttct ttcctggatg ccaacgcaaa atcccgccat
    13741 atccatatct ccaggctggt acatcccggg catttcagct gtttcaccac caattagagc
    13801 acacccgact tccttacagg ccctggcaat agaagccacc accttggtaa cttctttggg
    13861 atctaagtag gcagaagcca aataatcaag aaaaaacaga ggtcttgctc catgacataa
    13921 aatatcattt acacacatgg ccaccaggtc ctgtccaatg ttttcgctaa agcctgtggc
    13981 cttggccata agtatctttg ttccaactcc atcggtgccg ctaacaagaa ctggctcctg
    14041 caggccagaa ggcaggagaa aaagacccgc aaaggagccc agttctgtta aaacttcagg
    14101 tccttggctt gcttcaacat aagccttcat ttcttttacc gcatcttgtc cacggtctac
    14161 atccacccca gcgtctttat aagtgatttt catatttatt aagcaggaaa tttacccgcc
    14221 ttctcctttc gcaagactaa tatttctcga aaacatattt attatgggcc tttggcactt
    14281 ccataggata ctttcctgta aagcaggcct tgcacagggt gttttctggc aaaccaaccc
    14341 cttctataaa ttcttctgga gttaaaaagc gcagactatc agcaccaata tactcctcaa
    14401 tttcttctac cgacatattt gccgctatca gggatttttt actaggcgtg tctatcccaa
    14461 aaaaacaaga atagcctaca gggggtgagc tgatcctaac atggacctcc ttggcccctg
    14521 actcctttaa atcaataata atgctgcgca gggtggtccc ccgaacaatg gagtcatcta
    14581 caatcacaat tctcttgcct tggatattgc tcctaagaat gctcagtttt agtcgcaaca
    14641 tccgctctct tccttcttgg ctaggatcag taaaggtccg gccaaggtat ttgtttttaa
    14701 taaagacact agaataaact gtatctgcct ccatggcgta gccaatggca gaagagacac
    14761 cgctttcagg aacagctgct acaagatcat atttttcctt gtcttgtcta gccaggattc
    14821 gtcccagctc aatcctagtc tgatagacgt tttttcctgc taggtcgcta tcaggtctag
    14881 aaaaatagac aaattcaaag ccgcataaac tcattggttt tgtctgatca aaataatgcg
    14941 aatgatagcc tgtgtcatct atttcaatta tttctccctt atcgacctca cgaacaaatt
    15001 ttgcttttaa gaggtccagg gccactgtct cgctggccag gacataatca cccttgtctg
    15061 tttggcctaa taccaggggt cttagtccat aggggtctct gattccatag agcttgccct
    15121 ctacaataag aacaagagca tagcccccct taatctgtgc tagggccttt ttaatggctt
    15181 ctaggggatt tcccttatac tggattgcaa tcagtttaat aacaatctca ctatcactag
    15241 tggaggtaaa gctaaagcct tcatctatta gtcgctctcg tatttgtaca gcattgatta
    15301 ggttgccatt atgggctagg gccatggtcc cggctttaaa ttcagccact agtggttggg
    15361 catttcttct ttcagcgctt cctaaggtag aataacgaca atggccaatg cccacattgc
    15421 cattaaaagg acggaggttt tcttcattaa aaacatcacg aacaagaccc atgtttttat
    15481 aatgcatcat aacgccttca tggcagaccg aaattccagc actttcctgg ccccggtgct
    15541 ggaggctaat taaggcatca caaatctttg gtcccagatg ttgatactcc tgggagtaaa
    15601 tgcctacgat accacattct tcatgaagct tatctaagaa attattcatt ttttgtttcc
    15661 agttccctat catcctctag cactttttcc tccatctttt gaacataggt cctataggat
    15721 agtgcgattt tttcatcgct taaggcaagt atacgcaggg ccaaaagggc agcattctca
    15781 gcagcattta ctcctactgt tgccactgga atccctcctg gcatttgtaa aatacttagt
    15841 actgagtcca gaccacccat catggatgtt ttaatgggaa ttcctatcac aggaaggtct
    15901 gtagcagcgg cgattacacc gggaaggtgg gccgcttttc ctgctgcagc aataatcact
    15961 ccatagcctt ggccctttgc cccacgaaca aagtccaagg tggcatccag agttctatga
    16021 gcacttaaaa ttctcatttt gtgttcaaca gcaaattctt ctaaaatcaa agctgccttt
    16081 tctactactg gcagatctga tttacttccc atcactatgg caaccttcat tttgccctcc
    16141 taaaatgctc aagccctgca gaaaagatat ccatatcttt cttatgatct atattgacaa
    16201 aaagatctgg gccaagccgt tcattgtgtc ccatttttcc aaaaatacgg ccatcaggac
    16261 taagaagtcc ctcaatatcc atcattgatc cattgggatt atcaacataa accgtagcta
    16321 tttgttttct cgatacaaga agctgccggt cctcttcagt cataaaaaac cgtccttccc
    16381 catgggagat agggcaccaa tagttttgct cctcaacatt agacagccaa ggggaattct
    16441 gattcaagca acgaatccgt accagggccg attgatggcg accaagatca ttataagtaa
    16501 gtcccatagt taccttatca gcttcttcga ttcgtccatt ataaaaaacg cccaatttaa
    16561 caagggcctg gaagccattg catattccaa tcactagtcc tttttgatct actagctcgt
    16621 ggaaggcgtc cttaactttt ttagttctaa acaccgtggc aataaactta gcgcttcctt
    16681 ctggttcatc tgctgcagaa aagccgccag ccagtcccaa aatatcacat tcttttattt
    16741 ttttagccaa agtagttagg gaggagtcca tatctgatgt tgaaaagacc catctgtcaa
    16801 cttctgctcc ctcttcttca aatttatttc caaactcatc ttcgctattt gtacctggga
    16861 aaataggaat aaaaactttt accctttctt tttttccttc ttggagaaaa gatttctttt
    16921 ccattgcttt ttcaggcgct agagtcattc ctaattcaaa tataggatct agatgggctt
    16981 ctttcttttt aagggaggca tccagacatc ttttttctcc cttgagatga agctcggctt
    17041 cttctatcgt acgcccaaca agtcttgccc ctgtgagttt gcttcctgcc tccacctcta
    17101 caaggataga gccaagagga tggccatctt ctaatagctg gcagcccaga tgatttccaa
    17161 aggcggcatt ttctgctgtg tgaactagac ttaaggtaac tacactggcc gaaaggattt
    17221 tttcacggtc gctaatatag ccttctaaag ccttttttgc cccttctaaa tcaatacagt
    17281 aatcctctct taatggaatt tcatataaat aaagctcact gcctgccttt ttaaattcac
    17341 ttgttcgcac atgggcaagt tttcctgtat ttacacaaaa ggaaatcaag gttggaggta
    17401 catgaatttc tcccccctcc cagaaaaaac taccactcat actatctttg ccgccaatag
    17461 aagctagttt aaagtgatga aggacccgat tggcacctaa aagcgccata aatggcttgg
    17521 cccatttttt tggatctttt cctagggcct cgaaatactc ttggaaactt agtcgaaggc
    17581 ccttaagatc tcctccaagg acaaggttct tacttattga ctgcattaca gccatttcag
    17641 ccccatggaa gggactccac tgggccaaat aaggatcaaa accttggctg gcaatggtca
    17701 catcatctgt ttttactccg gggattttag aaaccattcc atccacaaaa ctttttcttc
    17761 tttttccacc ataggggctt actacattca ggccccctac actgctatca aacatttcta
    17821 ccagcccctg ctgggaggcc tcaagaagct ccttagagtc aagttctttc ttataggggt
    17881 ataagtctag ctttataggt tcaacctcaa catccgccct ctggggggct cctgctgctt
    17941 caataaagtc tctatggagg tctactagga ctttcccacc atggcttaca ataaagcgct
    18001 ccttgtctgt gatttttcca atcacagtaa ggcttaggtc ttcttccttg gcccttttct
    18061 taaaaagctc taaatctctg ggctcaataa ccagggccat cctttcttgg gactcgctaa
    18121 gcaggatctc ccaaggatag atttccttat actttgtagg aacatcctca atttctatct
    18181 caaggccagg ggcaatttct ccaaaggcaa cactaatgcc accagctcct aggtcattac
    18241 agcgtacaat ttttggtcct atatcttcca tgcggaaaaa acgctggagt tttctttctt
    18301 caggagcgtt tcctttttga acctcgctgg ctgctccggt ggctacgtct tcttgatggc
    18361 tttttgatga gcctgaagct cctccaatcc catccctacc tgtagctcct cctaataata
    18421 ataacaaatc tcccttttta ggtattcctc taacaacatg gcttctctta gcagatccaa
    18481 taacaaaacc tgcctccagt cgttttgctt caaaacctgg gtgatagtat tctttgacat
    18541 gcccagtaca aattccaatc tgatttccat aagaactata gccctgggca gctcttgtac
    18601 tgataatttg ttggggcagt tttcctggaa gcaccctggg atccttagga tctcgagcac
    18661 cgcttaccct cattgcttga tagacaaaac ttcgccctga taaagggtca cgaatggcac
    18721 caccaagaca ggtagcagcc cctccaaagg gttcaatctc tgttgggtga ttatgggtct
    18781 catttttaaa cataatcaga taatcttcgc cagcaaaatt cctctcgatg ctacaggcat
    18841 tgatttcttc actttcttcc aaatcttcca aatttccctg ggccttttct ttgcgcatac
    18901 taaatatggc aagctccata agacttgggg gttttttagt gtataaggag cggttttttt
    18961 cataataggc aaaaacctct tctgcaagag gaagattgtt cttagaaaaa tcaatctggc
    19021 aaagttctgt attaaaggtt gtatgccgac aatggtctga ccaataagta tctagaagtc
    19081 taagctcact ataggtcgga gcccgtcctt cttctttaaa ataatcttct ataacagcca
    19141 aatccgcacc ggtcatggca agattgtgtt taagacaaaa gtcctcccca tcaagtgtaa
    19201 agatatcctc catagactgg taggtaatat ccttatctct tcgaggttga gcaagggaaa
    19261 agtcttccaa atccctttca aagctttcca caggattgat aaaaaaatct tttacttcct
    19321 cccacttttc atcgggaagg cctttaaata gatatatatg tgaaaaataa atttcttctt
    19381 cttcttcaag tagctctagg catttagatg cccagtagct tctttggtca aattgcccag
    19441 gcagaaactc tgttcgtaaa tggcggtatc ctgtaggtgg ctcaaaagaa taagacttca
    19501 aatccagggc tgaatcaaag aaaatcttgt ttatgatttc ttcgctaaat tcctggggcc
    19561 tatagtaaag attgattatt cgtaaatcct ctagttcaaa accataaagg gcctcaatct
    19621 cacgctgtaa ttgtagattc ttagagcgaa attctggctt tttctcaaca taaattttat
    19681 tcattcttga acctcctaat accattttat actagtttgg tatataatta taattgtatt
    19741 tattaatact cttgataagg agggctacta tgcaaaactt agaatcctat cgcagtttct
    19801 atatggctgc acgttttgaa aatttcaccc gggccgcaga agaactcttt gtcacccagt
    19861 cttctgtttc ccaatcggta aaaaaacttg aaacaagtct aggcattgat ctttttttac
    19921 gccgaggtag gcgagtccag ctaacagaag aaggaaaagt tctctataaa gaactagata
    19981 aggtgtttaa gaccctagaa gatgctgaac gcctaatgat ggaatataaa agtgttgaaa
    20041 agggcagtct atctcttgct gccagtgata ccatatgtcg ccactattta ttacctgttc
    20101 ttaagtccta taaagaagag tttcctcata ttcatataaa tattatcaat cagccttcac
    20161 ctattgtagc tgaactagtg cgtacggggc aggttgatct tggttttgtc aatgggcaaa
    20221 gttctgatta tagtgatttg gttccacttg cccttttaaa tctaaaagaa cagtttttct
    20281 gcgcagaaaa aagtcccatc cccaaaagaa cactctcctt agaggagctt ttggattatc
    20341 cccaggtttg tttaaaacaa caaacatcta caagacagct tttggatgat ttgttccgaa
    20401 aagagcgcct taaattccag cccgaggtag aggttattag tattgatcta ttgattgatt
    20461 taattagagt gggttttggc attggtttta gccatagcca tttaataaaa aaagagggtc
    20521 tgcgattaat caaggtagat caagatcttc ctcagcgcaa actcctcctt ttaacaaatc
    20581 ctagaatctc tccttccaaa gctggtggac tctttttaga cctgtgtcat agttttttct
    20641 tttgtcatag tgcttaatta aacctttttt gaataagaaa tcccgctgct gctaaggcta
    20701 tacttaaaaa aacaaggcca tgggagcttt tttctcctgt tttaggtagt tctcttttat
    20761 taacaagctc aattagaaga ggagaattca ccccataatt ttcaaagttg attttctgct
    20821 cttcacaggg gatgtagcct cttaagcttc gagtttcctt taggatataa tccccttctg
    20881 gaagatagtc tatagatata gtc
//
LOCUS       DASRNA010000171         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_798640,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000171 DASRNA010000000
VERSION     DASRNA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798640"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VFD08_04770"
     CDS             <1..991
                     /locus_tag="VFD08_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK10363.1"
                     /translation="ARDREKKIERMEVVQAPLWISRDMSLRFKAARATGQDILTCENL
                     SKSFGKETLFEDLSFSLYKGDVLGIIGPNGSGKTTLMRLLARETRPDQGRIRFGPGVD
                     PGFYRQHQEELRTSEDMVTFINNEFSQYNHGEIRSLLAQFLFTGEEVFKSIKLLSGGE
                     KARLRLLLLMLEERNLLLLDEPTNHLDIHSKQTLEEALLHYEGTIILVSHDRYFLNRV
                     CNRLLVLDGEDSGTFEGSYKDYEAKPSPTREEKTKKSKAKTPRKTPVRSQSQADPIED
                     KLLLLEKELEEIELAFLDNEIYNDNDKIKKLSVKRDNCLQKIEKLYDLWGKEE"
     gene            992..1216
                     /locus_tag="VFD08_04775"
     CDS             992..1216
                     /locus_tag="VFD08_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZK10364.1"
                     /translation="MFETVQRIISEQMGVDKASITKKTRLIEDLRADSIDAAEMILTI
                     ESELDLLVADDDVMDVKTVQDILDLLEKAK"
     gene            complement(1286..2638)
                     /gene="glmM"
                     /locus_tag="VFD08_04780"
     CDS             complement(1286..2638)
                     /gene="glmM"
                     /locus_tag="VFD08_04780"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZK10365.1"
                     /translation="MGIYFGTDGVRGIAGKELTNELAYKLGRYGAYVITKHSPREKAR
                     LILGKDTRVSSYMLESAMIAGILSAGCDVVQVGVIPTPGIAHLVKTEGFDGGIMVSAS
                     HNSYEYNGIKFFNEKGLKLTDKIEEEIESYITGERSIDKEILGDAIGRLEIRKELRAK
                     YLAHAEELCHEDLGSLRILLDTANGATHKLGPLAFKAMGAKVDVIFNSPNGVNINDMC
                     GSTHLGTLSKKVVKGGYDLGLAFDGDGDRVLAIDSLGREVDGDGIMAICALDLLERGE
                     LKNEGLVATVMSNLGFLNYMKEKGVDVTIAPVGDRYVLEKMLEGGQILGGEQSGHVIL
                     LHKNTTGDGIQTGLLLASALIKSGKSLAAWRDEIPKLPQYLTNARVSKDKQGLYKELA
                     RAQKKIKEIEDILGDRGRVLIRPSGTEPLIRVMLEGPEEDLIKDLAEQLKEIYLEELA
                     "
BASE COUNT          789 a          615 c          506 g          776 t
ORIGIN      
        1 ggctcgtgac cgagaaaaga aaattgagcg aatggaagta gtccaggctc ccctttggat
       61 ctcaagggat atgagcctgc gctttaaagc agcccgagcc acggggcaag atattcttac
      121 ctgcgaaaat ctttcaaaat cctttggcaa ggaaactctt tttgaagact taagcttttc
      181 cctctataag ggggatgtcc taggaattat tggtcccaat ggatcaggga aaactaccct
      241 gatgaggctg ctggctagag aaacaagacc tgaccaggga agaatccgct ttggtcctgg
      301 ggttgaccca ggcttttacc gccagcatca agaagaactt agaacttctg aggatatggt
      361 cacctttatt aacaatgaat tttcccagta taaccatgga gaaattcgct cactactcgc
      421 ccaatttctt tttaccggcg aagaagtctt taaaagcatc aagctcttaa gcgggggaga
      481 aaaagcccgg ctcagactcc tgcttcttat gctagaagag cgtaatttgc ttctacttga
      541 tgagcctacc aaccacctag atattcacag caaacaaacc ctagaagaag cactcctcca
      601 ctacgaagga acaattatcc tggttagtca tgaccgctac tttttaaata gggtttgcaa
      661 ccgacttttg gtccttgatg gagaagattc tggaaccttt gagggaagct acaaagacta
      721 tgaagctaag cctagcccta ccagagaaga aaaaacgaaa aaaagtaagg caaaaacacc
      781 cagaaaaact ccagtgagat cccagtctca agcagatcct atagaggata aacttctctt
      841 actggaaaag gagctagaag aaattgaact tgccttctta gataatgaaa tctataatga
      901 taatgataag ataaaaaaac tatctgtaaa aagagacaat tgtctgcaaa aaatagaaaa
      961 actctatgac ctttggggaa aggaagaata aatgtttgaa accgtacaaa gaattatttc
     1021 agaacaaatg ggggttgata aggcctctat cacaaaaaaa acaagactga ttgaggacct
     1081 tcgagcagac tccatagatg ctgccgagat gatcctaact attgaaagtg aacttgatct
     1141 tcttgtagcc gatgatgatg ttatggatgt aaaaacagtc caggatattc tagacctact
     1201 agaaaaagca aaataaactc ccaaaccagg gagtttattt ttttaccttt tatatccttc
     1261 ttattatttt cttcttagaa ataagttatg ctagctcttc taagtaaatt tcctttagct
     1321 gctcagccaa atcctttatt aggtcttcct ctggcccttc gagcataacc cgtatcagag
     1381 gctctgttcc tgagggacga ataagaaccc ggcccctatc tcctaaaatg tcttctattt
     1441 cttttatttt cttttgagct ctagctagtt ccttataaag gccttgcttg tccttgctca
     1501 ccctagcatt ggttaaatat tggggaagtt ttggtatctc atctcgccag gctgcaagac
     1561 tctttccgct ttttattaga gccgacgcaa gaagaaggcc ggtttgtata ccatcacctg
     1621 ttgtgttttt atgaagtaaa atcacatggc ctgattgctc tccccctaat atttgtcccc
     1681 cctcaagcat tttttcaaga acgtatctat ctcccacagg ggcaatcgta acatcaaccc
     1741 ctttttcttt catataattc aaaaagccta aattgctcat tacagttgct acaaggccct
     1801 cgtttttgag ctctccccgc tctaagaggt ccagggcaca gatggccata atgccgtctc
     1861 catcaacttc tctgcctagg ctatcaatag caagcacccg gtctccatcc ccatcaaagg
     1921 caagaccaag gtcatagccg ccctttacta ccttcttgct aagggttcct agatgggttg
     1981 agccgcacat atcattgata ttgactccat taggggaatt aaaaatcaca tctaccttgg
     2041 cccccatggc tttaaaggcc aagggtccaa gtttatgggt ggctccattg gctgtatcaa
     2101 gaagaatccg taggctgcca agatcttcat gacaaagttc ttcggcatgg gccaggtatt
     2161 ttgctcttag ttcttttctt atctcaagtc ggccaatagc atcacctaga atctctttat
     2221 ctatggacct ttcccctgta atatagcttt ctatttcctc ttcaatttta tctgttagct
     2281 ttaatccttt ttcattaaaa aacttaattc cattgtattc ataggaatta tgggaagcac
     2341 tgaccataat ccccccatca aaaccttctg tttttaccag gtgggcaatg cctggagtgg
     2401 ggataacccc aacctggacc acatcacaac ccgcacttaa aatacctgct atcatagcag
     2461 attcgagcat ataggaagaa actcttgtat cttttcctag gattagtctt gctttttccc
     2521 taggtgaatg tttggtaatc acataagcgc catagcgacc aagcttatat gcaagttcat
     2581 ttgtaagctc tttgccggca attccacgta ctccatctgt tccaaagtat attcccatgg
     2641 taactccttt cactatttat taattctaca ataaataggg gatatt
//
LOCUS       DASRNA010000172         4889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1019868,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000172 DASRNA010000000
VERSION     DASRNA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019868"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFD08_04785"
     CDS             complement(<1..592)
                     /locus_tag="VFD08_04785"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZK10366.1"
                     /translation="MNKRKSGLLLALSSLPGPYGIGGFGQEALAFIDILHRAEQGYWQ
                     ILPLGPTGFGNSPYSSFSTQAGNPLYIDLEDLGKKGLLLREELDHAKRPQGRVDYTNL
                     HDERLALLKKAFSRVQRPLEEEIRLYAQNNPWLDDFALFMCLKDLHQGRSWLDWPDEY
                     RNRKPQALEKIREQEKVNYQFWIFTQFVFHKNMKDFN"
     gene            734..2050
                     /locus_tag="VFD08_04790"
     CDS             734..2050
                     /locus_tag="VFD08_04790"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018921811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZK10367.1"
                     /translation="MLNFKNSPYSLEELEKHQAKITAIHTSMEARTSLGAEYLGWLKA
                     ASTFEEKELKRLIQLASKIREEADLLVVAGIGGSYLGADALIQALKPTFGQDKPQILF
                     CGNNLSAAYMEELLAYLEGKCFYVNVISKSGRTVETALAFRFLKKELTKRYGQEAGRY
                     IIATTDAKSGALRRQVEEEGYKSFVVPQDIGGRYSVMSPVGLFPMAVAGLDVKGFLAG
                     MTQAEKDFSSPKLEENAAYRYALTRRLLYKKGKIMEILVTYEPALEKFAEWYKQLFGE
                     SEGKEEGGIFPVAVANTTDLHSMGQIIQDGPKIFFETVVDLSKPSSLVLPEVKAEDDL
                     DYLQGQKISGMTKKALLATLIAHEEAGVSNLLIEMESYSEESLGYLMYFMMRACAMSA
                     YLGGVNPFDQPGVEAYKNNMLALLGNPNYKKYKADIEKKLREKNLV"
     gene            complement(2074..2676)
                     /locus_tag="VFD08_04795"
     CDS             complement(2074..2676)
                     /locus_tag="VFD08_04795"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZK10368.1"
                     /translation="MNYHKIVPKEIGNILPYKQFSVAIILHKIQGKEYVILEKRSPNL
                     VYQPSDISLPGGKIEPGETKEEAAIRESCEELHLKPQELEVYGQLDRLHTPYGVEVSV
                     FFMRLNKKEKISSWSLDEVEKVFYYPLEELLKKVPTYYVETSHDFNDDFPFEYIQGGK
                     DYPFKTMKLPYYFYPVEDTWVWGLTARILHYFGEKIKEIL"
     gene            2745..3302
                     /gene="folE"
                     /locus_tag="VFD08_04800"
     CDS             2745..3302
                     /gene="folE"
                     /locus_tag="VFD08_04800"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZK10369.1"
                     /translation="MNREKIEKAVRMILEAVGEDPDREGLQDTPARVARMYEEIFNGL
                     KEDPGRHLRIQFTEEGHNELVLVKDIPFYSTCEHHLVPFFGQAHIGYIPEGGRVVGLS
                     KLARALEDVARRPQLQERITSSLADVIMEEVNPWGVIVVIEAEHLCMSMRGIKKPGSK
                     TVTSAVRGGFRSDARSRQEALELIR"
     gene            3389..3757
                     /gene="folB"
                     /locus_tag="VFD08_04805"
     CDS             3389..3757
                     /gene="folB"
                     /locus_tag="VFD08_04805"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZK10370.1"
                     /translation="MDKLILKDIRLYGYHGVFEEEKNQGQPFLLQAEIWIPERKSREE
                     DLTTTVNYAGCYEEIKSVFETPHLLLENLALAIIEALFSYDKRIKKVSIRIEKLRPPV
                     SGDLASLGVVLCRERQSFYL"
     gene            3727..4218
                     /gene="folK"
                     /locus_tag="VFD08_04810"
     CDS             3727..4218
                     /gene="folK"
                     /locus_tag="VFD08_04810"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZK10371.1"
                     /translation="MPRASVFLSLGSNQGQRKLLLRRALEELDSLEATQVSRISSLYE
                     SKAWGKTDQEDFYNLVILLETDFTPMELLSRTQEIEKKLGRVPTERWGPRTMDIDLLL
                     YNDICLDEPRLRLPHPYMLQRRFVLEPLLEIAPELCIDNKPLEDYIEVVNDKPLKKIG
                     RLW"
     gene            4262..>4889
                     /locus_tag="VFD08_04815"
     CDS             4262..>4889
                     /locus_tag="VFD08_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZK10372.1"
                     /translation="MNQGIIYSSYLAFYLLLSIFPFIMAVVAIMGFIPFELEGFFDRF
                     IGIFPQEVHLNLKNFVESNRRNSNLIGYSMFFLLWSSARAIGAIRKSLDMISGEKSRH
                     NFIKQRFFDSVRNFAFIFLLLIIFLIPTIIKLTKLGTLLLKINIPYFLTLIDTFQWVF
                     ILAMLFGIISLIYMRMGTKKYNFREVWRGALLAAIGWSIMSYLFNGILS"
BASE COUNT         1457 a          927 c         1075 g         1430 t
ORIGIN      
        1 cattaaagtc tttcatattc ttatgaaaga caaattgtgt aaatatccaa aattgatagt
       61 tgactttttc ttgctctcgg attttctcca gtgcctgggg ctttctattt cgatattcat
      121 ccggccagtc tagccaggaa cggccctggt ggaggtcctt taaacacata aatagagcaa
      181 agtcatctag ccaggggtta ttctgtgcat agagacgaat ctcctcttct aggggccttt
      241 ggacccttga gaaggccttt ttaagcaggg ccaggcgctc atcgtgcaag ttagtataat
      301 caacgcgtcc ttgagggcgc ttggcatgat caagttcttc tctaagaagg aggccttttt
      361 tgcctaagtc ttctaaatca atatataagg gattgccagc ctgggtagaa aaactactat
      421 agggagagtt tccaaagccc gtaggtccca aaggcaaaat ttgccaatag ccttgctcgg
      481 ccctgtgtag gatatcaata aaagctaagg cctcctgtcc aaaaccaccg attccatagg
      541 gacctggaag ggaggatagg gctaggagca aaccactttt tcgtttattc ataaatcact
      601 ccttaaagat caaataattt accttatcat agcatagaga gggatttaaa tatatatgcc
      661 agtcccttaa gagctgctga aattattgtt ggcctatgtt aaaatcaact cagaagaagc
      721 gaaaggagtc attatgctta actttaaaaa tagcccatat tctttagagg aattagaaaa
      781 acaccaagca aaaattactg caattcatac ttccatggaa gctagaactt ccctaggggc
      841 agagtattta ggatggctaa aggcagcttc tacttttgaa gaaaaagagc tgaaaagact
      901 gatccagctt gctagtaaaa ttagagagga agcagatcta ctggttgtgg ctggtatagg
      961 gggctcctat ttaggggctg atgcactgat ccaggcctta aagcccacct ttggccagga
     1021 taaaccccaa atcttgtttt gtggaaacaa tctaagcgca gcctatatgg aagaactctt
     1081 agcctatcta gagggtaaat gtttttatgt caatgtgatt agtaaaagtg gacgaaccgt
     1141 ggaaacagcc ctggcttttc gttttttaaa aaaggagctt actaaaagat atggccagga
     1201 ggcaggaagg tatattattg caaccaccga tgccaaaagc ggcgccctta gaaggcaggt
     1261 cgaggaagaa ggctataaga gctttgttgt tccccaggat atagggggac gctactctgt
     1321 gatgagtcct gtgggtctat ttcccatggc agtagctgga ctggatgtaa agggctttct
     1381 tgcgggcatg acccaggcag aaaaggactt ttcttcccct aaactggaag aaaacgcagc
     1441 ttatcgctac gcccttacgc gcagactgct ctataaaaaa gggaaaataa tggaaattct
     1501 tgttacctat gagcctgctt tagaaaagtt tgccgagtgg tataaacaac tctttggtga
     1561 gagcgaggga aaagaagaag gggggatctt ccctgtggct gtagccaata ccacagacct
     1621 tcattccatg ggacaaataa tccaggatgg tcctaagatt ttctttgaaa cagtggttga
     1681 tctttctaag ccgagttctc ttgtgctgcc tgaggtaaaa gcagaggatg atctggacta
     1741 tctccagggg caaaaaatta gtgggatgac aaaaaaagcc cttctagcta ccttaattgc
     1801 ccatgaagag gcaggggtga gcaatctact aatagagatg gaaagttata gtgaagagtc
     1861 cctaggctac ctaatgtatt ttatgatgag agcttgtgcc atgagtgcct atcttggagg
     1921 ggtaaacccc tttgatcagc ctggagttga agcctataaa aacaatatgt tggccttgct
     1981 gggcaatcct aattataaaa aatataaagc agatatcgag aaaaagctaa gggaaaaaaa
     2041 tcttgtataa gaaagccttg ataaagatca aggctataaa atctctttta ttttttctcc
     2101 aaaataatga agaattctgg cggtaagccc ccaaacccat gtatcttcta caggataaaa
     2161 atagtagggg agcttcattg ttttaaaggg ataatccttt cccccttgaa tatattcaaa
     2221 gggaaaatca tcattaaaat catgagatgt ctccacatag tatgtgggga ctttttttag
     2281 cagttcttct aaagggtaat aaaagacctt ttctacttca tctagactcc agcttgagat
     2341 tttttctttt ttatttagtc tcataaaaaa aacagaaact tccaccccat agggtgtatg
     2401 gaggcgatcg agttgaccat agacctctag ctcctggggc ttgagatgga gctcctcgca
     2461 gctttctcta atggctgcct cttcttttgt ttctccaggt tcaatttttc ctccaggcag
     2521 gctgatatct gaaggttgat agacaagatt gggactgcgc ttttctaaaa tcacatattc
     2581 ttttccttgg attttatgaa gtataatagc aacgctaaat tgtttgtagg gcaatatatt
     2641 tcctatttct ttagggacga ttttatggta gttcattttc tcctcctagg gtaagtatag
     2701 caaagttggg gtatcttttg tatactagat taaggaggcg aaaaatgaat cgagaaaaaa
     2761 tagaaaaagc tgtgcgcatg atactagagg ccgtaggaga agacccagat cgtgaaggac
     2821 ttcaagatac tcctgccagg gttgcacgta tgtacgagga aatctttaat ggcctcaaag
     2881 aagatcctgg tcgtcattta aggatacagt ttacagaaga aggtcataat gaacttgtcc
     2941 tggtaaagga cattcccttt tattctactt gtgaacatca cttggtgcca ttttttggcc
     3001 aagcccatat tggatatatt cctgaagggg gaagagtggt tggcttaagt aagctggcaa
     3061 gagccctaga agatgttgcc agaagacccc agctacaaga aagaattacc tcctctcttg
     3121 cagatgttat tatggaagaa gtaaatccct ggggcgttat tgtagtgatt gaagcagaac
     3181 acctttgcat gagtatgcgg ggcattaaaa aaccaggaag taaaacagtt acttcagcag
     3241 tacgcggtgg ttttcgcagc gatgcaagga gccgccaaga ggccctagag ctgattcgat
     3301 aagattacca agatacagac cgcctttggc ctcttaaagg accctactgg cttggcctgc
     3361 ccttttttag aatagaaagg tttactttat ggataaatta atactgaagg atatacgact
     3421 ttatggctat catggtgttt ttgaagaaga gaaaaatcag ggacaaccct ttttacttca
     3481 agcagaaatc tggataccag aaagaaaaag cagggaggaa gatcttacaa caacggttaa
     3541 ttatgccggc tgttatgaag agataaagtc tgtttttgaa accccccatt tgcttctaga
     3601 aaaccttgct cttgcgataa tagaagctct tttttcctac gacaaacgaa tcaaaaaagt
     3661 ctctattagg attgaaaaac ttaggcctcc tgtctcaggg gaccttgctt cactgggggt
     3721 ggtcttatgc cgcgagcgtc agtcttttta tctttaggta gcaatcaggg ccaaagaaag
     3781 ctattattaa gaagagccct agaggagtta gattctctag aagcaacgca ggtttctcgt
     3841 attagcagtc tttatgaaag caaggcttgg gggaaaaccg atcaagaaga tttttataat
     3901 ctggttattt tgcttgaaac agattttaca cctatggagc ttctaagcag gacccaagag
     3961 atcgaaaaaa agcttgggcg ggtgcctact gagcgctggg gtcctaggac catggatatt
     4021 gacctcttat tatataatga tatttgcttg gatgagccca ggcttcgcct gccccatccc
     4081 tatatgttac aaagacgttt tgtcttagaa cccctattag aaatagcccc agaactttgc
     4141 attgacaata agccactaga agactatatc gaggtagtta atgataagcc ccttaaaaaa
     4201 attggaagac tatggtaaca aatactacac attgaccact gaggtaattc agaggtttac
     4261 tatgaatcaa ggaattattt acagttctta tttggctttt tatttattgt tgagcatatt
     4321 tccctttatt atggcagttg tggccataat gggttttatt ccctttgaac tagaagggtt
     4381 ttttgatcgc tttattggaa tattccccca ggaggttcat ttaaatctta agaactttgt
     4441 tgaaagcaat cgccgcaata gtaacttaat tggttattcc atgtttttct tattatggtc
     4501 ctcagcaaga gccattgggg ccattagaaa atctcttgat atgatttcag gagaaaaaag
     4561 ccgacacaac tttatcaagc aaaggttttt tgattcggta agaaatttcg cctttatctt
     4621 cttgctacta ataatctttc ttatccctac aataattaaa ctaacaaagc taggtaccct
     4681 cctgctaaag atcaacattc cttacttttt gaccctaatt gacacctttc agtgggtttt
     4741 tattcttgct atgctttttg gtattatctc tttgatctat atgcgaatgg ggacaaaaaa
     4801 atacaatttt agggaggttt ggcggggggc tcttttagca gccataggct ggtctattat
     4861 gagttattta tttaatggca tcctcagcc
//
LOCUS       DASRNA010000173         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_649044,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000173 DASRNA010000000
VERSION     DASRNA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649044"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..723
                     /locus_tag="VFD08_04820"
     CDS             91..723
                     /locus_tag="VFD08_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HZK10373.1"
                     /translation="MDYIHLYTRQHENSIYELEQKGRITNKEIYIKLHMRDIAPFFLE
                     KYSTFTQMAQKRLPRPKDVNYPIWCSVSKENCLRPIEKELVYALRVPKKELIYFDGGK
                     WDFVLNNLYLPKNKEDEAAYLRELKKYGINNSFELLLDKNKGIYLIFEKRIKESWERI
                     FDIDDWNPFIVQANLWQIKEEWVKHILGPGEDLFALEDMEETFPPKDLIF"
     gene            825..1457
                     /gene="pcp"
                     /locus_tag="VFD08_04825"
     CDS             825..1457
                     /gene="pcp"
                     /locus_tag="VFD08_04825"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HZK10374.1"
                     /translation="MKILITAFDGFGGEKINPTAKALEKLGPLENVQLKKLLLPTVFG
                     DSLEILKKEIREFNPQTVVCLGQAGGRTGISPERIAINIIDARIPDNKGNSPIDQPIN
                     PKGPAAYFSTLPIKAMVKTLQDHGIPSSVSNTAGSFVCNYIMYGLLDEIKDQPNMRGG
                     FVHLPYLPEQVVDKNLPSMSLTDLVRALELLIQSLTIYKEDLLLSGGQED"
     gene            complement(1502..>2521)
                     /locus_tag="VFD08_04830"
     CDS             complement(1502..>2521)
                     /locus_tag="VFD08_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HZK10375.1"
                     /translation="AFVGLISGTWEPLIWMLIYLSFNVFVILRGINKGIEAASKILMP
                     TLFVLLLIIIIKGLTLPGAAVGLSFLLKPDFSKVTGATFLAALGQAFFSLSLAMGVMT
                     TYASYLGEGESIPKNAVTVSIMDFSVALMAGLAMFPALFAFGMEPTQGPGLVFMVVPK
                     LFQAMGGVGPIFSFIFFAALMVAALTSSMSLLEVVVSFLIDQKKMTRKKATGIAAGSM
                     FVICVFASLSQGPLSHITLFGANTFDFLDLVTDKIFLAVGGFFISVFIGWKVDKDLLF
                     NEFTNHGTNSVGMFSVWYNLIKYVLPVVIAVVAVTGIASIAQTGVMVTGLVIIALLAA
                     FSKKI"
BASE COUNT          880 a          508 c          458 g          675 t
ORIGIN      
        1 gcttatatat ttataataaa gtaaaagctc tgtcgctttt ggtctttttt agggtaaaaa
       61 gtaagcaaga gccaaagtaa ggaggaaaat atggactata tacatctata tacccgacaa
      121 catgaaaatt caatttatga actagaacaa aaaggtcgaa ttacaaataa agaaatttac
      181 atcaaacttc atatgcgaga tatcgctccc ttttttttag aaaaatactc cacctttaca
      241 caaatggcgc aaaaacgtct accccgaccc aaggatgtta attatcccat atggtgctct
      301 gtaagtaaag aaaactgcct tcgtcccatt gaaaaagaac ttgtctacgc ccttagggtc
      361 cccaaaaaag aacttattta ttttgatggg ggaaaatggg actttgtcct aaacaatctc
      421 tacctgccaa agaataaaga ggatgaagcg gcctatctaa gggagcttaa aaagtatggt
      481 attaacaatt cctttgaact cctccttgat aaaaacaagg gtatctattt gatttttgaa
      541 aaaagaatta aggagagttg ggaaagaatc tttgatattg atgattggaa tccctttatc
      601 gtccaagcca atttatggca aatcaaagaa gaatgggtta aacatatcct tggacctgga
      661 gaagatctct tcgccctaga ggatatggaa gaaacctttc ctccaaagga tcttatcttc
      721 tagttttttc ttaccataat ttatacaatt actaggagca actatctttt agtgatcaat
      781 ctaatgatat gctctaacta gctaccatca tagaaggaga agatatgaaa atacttatca
      841 ctgcttttga cggctttggg ggagaaaaaa taaaccccac tgctaaggcc ctggaaaaac
      901 taggtcccct ggagaatgtt caactcaaaa aacttctttt acccaccgtc tttggtgatt
      961 cattggaaat tctaaaaaag gaaatccggg aatttaatcc ccagacagta gtttgcttag
     1021 gacaagcagg gggtcgtaca ggaattagtc cagagcgcat agctataaat atcattgatg
     1081 caagaatacc cgataataag gggaactcac ccatcgacca gcccataaat ccaaagggac
     1141 cagcagctta cttttctact ttacccataa aagctatggt aaaaaccctg caagaccatg
     1201 gtatcccttc tagtgtttct aatacagcag gtagctttgt atgtaattat ataatgtatg
     1261 gacttctaga tgaaattaaa gatcagccaa atatgagagg gggctttgtc catcttccct
     1321 atctgccaga gcaggttgta gataaaaacc tcccctccat gtccctaaca gatcttgttc
     1381 gcgccctgga actccttatt cagagcctta ctatatataa ggaggacctc ctgctttcgg
     1441 gaggacaaga agactaaatt taggcaaaaa aaaggagcca aggctccttt tttagtattt
     1501 cttagatttt ctttgaaaaa gcagctaata gtgcaataat cacaagtcct gtaaccataa
     1561 caccagtttg tgcaatagag gctatacctg taacagcaac tacggcaatc actacaggaa
     1621 gaacgtattt aatcaaattg taccaaacag aaaacattcc aacggaattt gttccgtgat
     1681 tagtaaattc attaaatagt aaatctttat caactttcca tccaataaag acactaatga
     1741 aaaatccgcc cacagctaag aaaatcttat ctgttactaa gtcaagaaaa tcaaaggtat
     1801 ttgcaccaaa taaggtgatg tgtgataagg gtccttggct aagtgaagca aagacacaaa
     1861 taacaaacat agaacccgct gcaattcccg ttgctttctt tcgcgtcatt tttttctggt
     1921 caattaaaaa gctaacaacc acttccaaaa gtgacatact acttgtaagg gctgcaacca
     1981 ttaaagctgc aaagaatata aagctaaata ttggtcccac tccacccata gcttggaata
     2041 gctttggtac aaccataaag acaagaccag ggccttgagt gggctccatg ccaaaagcaa
     2101 atagagctgg aaacattgct agacctgcca taagggcaac agaaaagtcc atgattgaaa
     2161 ctgtaacagc attttttggt atggattcac cttcgccaag ataagaggca tatgtagtca
     2221 taacgcccat agcaagactt agggagaaaa aggcctggcc caaagctgct aagaatgttg
     2281 cccctgtaac tttagaaaag tctggtttaa gtaaaaagct caagcctacc gccgctccag
     2341 gaagcgttaa acccttgata atgataatta acaagagcac aaataaggtt ggcataagta
     2401 ttttagatgc agcctcaatg cccttattta ttcctctaag tataacaaag acattaaagg
     2461 ataaataaat aagcatccag ataagtggct cccatgttcc tgagattaac cccacaaaag
     2521 c
//
LOCUS       DASRNA010000174         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_649124,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000174 DASRNA010000000
VERSION     DASRNA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649124"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VFD08_04835"
     CDS             complement(<1..155)
                     /locus_tag="VFD08_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009670090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HZK10376.1"
                     /translation="MDKEIRLNKYIRDSGFCSRREADKYIEAGCVLVNKKPASIGTRV
                     GPGDKVY"
     gene            complement(227..1570)
                     /locus_tag="VFD08_04840"
     CDS             complement(227..1570)
                     /locus_tag="VFD08_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK10377.1"
                     /translation="MDISNTVKKLGINKMIDYALKDPKKNFEKILDWADTFAGDDFAS
                     QRKGVRQAMTNPESPYYDFIHQSVERIDQHVLKTLSRNLFFNASLIGGPIHEKLRQEY
                     NCNIPWTILLDPTSACNMNCKGCWAAEYGNKLNLTYKEIDDIISQGKELGVYMYIYTG
                     GEPLVRKNDLIDICEKHSDCVFLTFTNGTLIDDDLAKEMLRVGNLVPAISLEGFEEAT
                     DSRRGPGAYQKVMDAIEVLRQNKLLYGISTCYTSENIDSILSDEFTDHLIELEAAFIW
                     YFHYMPVGKGALPELLPSPEQRKRVFYKIRKNRKEKPLFAMDFQNDAQYVGGCIAGGR
                     DYLHINAAGDIEPCVFIHYSDSNIREKTLLEALGSPLFMAYHDKQPFNENMLRPCPML
                     ENPDKLREIIKNTEAKSTNLLSSESADELCSKCDRYAKNWAPTADEIWKEERAGL"
     gene            complement(2080..3738)
                     /gene="ilvD"
                     /locus_tag="VFD08_04845"
     CDS             complement(2080..3738)
                     /gene="ilvD"
                     /locus_tag="VFD08_04845"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZK10378.1"
                     /translation="MKSHVVTLGVEKAPHRSLLKSMGLTAEEIRRPLIGVVNTFNEIV
                     PGHKHLRQVAEAVKAGVLMEGGTPLEFPAIAVCDGIAMNHQGMKYSLVSRELIADSVE
                     IMTLAHGLDGLVMIPNCDKVVPAMLMVAARLDIPALIVSGGPMLAGRHRGKKIDLSTM
                     FEAVGAVNAGKISEEELYEMEDAACPGCGSCSGMFTANSMNCMTEALGMALPGNGTIP
                     AVFGERMRLAKYSGMQIMKLVEKDIRPSQILTPAAFDNALVVDMALGCSTNTVLHLTA
                     IAYEAGVDMNLDKINEISEKTPNLCRLSPAGPDHIEDLYYAGGITVLMAELSKKNLLD
                     LSAQTVLDKTIGEITKGQKRNDSPVIRSTENPYSQSGGIAVLKGNLAPEGAVVKRSAV
                     AKEMLRHSGPARIFEGEEEAVEAIRSGKIHKGDVIVIRYEGPRGGPGMREMLSPTSTL
                     AGMDFDKDVALITDGRFSGATRGAAIGHVSPEAALGGPIAFLEEGDKIEIDMIKGVLH
                     VDLSEEELAQRKKNWQPKENKQKGYLEKYRKIVTSASTGAICKP"
     gene            complement(3740..4732)
                     /gene="ilvC"
                     /locus_tag="VFD08_04850"
     CDS             complement(3740..4732)
                     /gene="ilvC"
                     /locus_tag="VFD08_04850"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZK10379.1"
                     /translation="MSKMYYAEDCKVELIQGKKVSIIGYGSQGHAHALNLHESGVDVT
                     VGLYLASKSWKKAEEAGLKVRTVAEAVKETDVIVLLVSDERQVELYESCIKDNLTRGK
                     YLVFAHGFNVHYGQILPPEDVNVFMVAPKGPGHTVRSQFLEGRGVPCLATVYQDVTGD
                     TMELALSYAAGIGGARAGVLETTFKEETETDLFGEQAILCGGVTELMKMGFEVLVEAG
                     YDPASAYFECVHEMKLIIDMVNEGGLSYMRYSISDTAEYGDYSVGKRIITEETRKEMK
                     KVLAEVQDGTFAKNWILENKANRPFMKAKRRQEQNHPMELVGKKLRKMMSWLKK"
     gene            complement(4777..>4935)
                     /locus_tag="VFD08_04855"
     CDS             complement(4777..>4935)
                     /locus_tag="VFD08_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZK10380.1"
                     /translation="RGSVLDVSSSSLTILLTGDEKKLDGFVEMMKSYEILEIAKTGIL
                     AMSRGEKM"
BASE COUNT         1214 a         1144 c          910 g         1667 t
ORIGIN      
        1 atatatacct tgtctccagg ccctaccctg gttccaattg aagccggctt tttatttact
       61 aaaacgcagc ccgcttcaat atatttatct gcttctcttc tagaacaaaa gcctgaatct
      121 ctgatgtatt tatttaatct gatttctttg tccattctta tttgttcctt catttatggg
      181 gtttgtttta ttgtaaacaa aaagcttggt cttttgcaag caggtcttat aggcccgccc
      241 tttcttcttt ccatatttca tctgctgtgg gagcccagtt tttagcatat ctatcacatt
      301 tagagcagag ctcatctgca ctttctgagg aaagtagatt tgtagacttg gcctctgtgt
      361 tttttattat ttctctaagt ttatctgggt tttccagcat aggacagggt cttaacatat
      421 tctcattaaa gggttgctta tcgtggtagg ccataaataa gggggagcct agggcctcta
      481 ataaagtctt ttctctaata ttagaatcag agtagtgaat aaacacgcag ggctctatat
      541 ctccagccgc attaatatgt aagtagtctc tgccccctgc gatgcatcct cctacatact
      601 gggcgtcatt ttgaaagtcc atggcaaaaa gtggtttttc cttgcggttt tttctgattt
      661 tataaaaaac tctttttctt tgttctggac taggcagaag ctctggtaaa gcgcccttac
      721 ccactggcat atagtggaag taccagataa aagcggcctc tagctcaatt aagtgatcgg
      781 taaattcatc agataaaata gaatctatat tctcactagt atagcaagta gaaatgccat
      841 aaagcaattt attttggcgt aaaacttcaa ttgcatccat caccttttgg taggcccctg
      901 gtccacgcct actatctgtt gcttcttcaa aaccctctag ggagatggct ggtacaaggt
      961 tgcccaccct tagcatttct tttgcaaggt catcatcaat tagtgtacca ttggtaaatg
     1021 ttaaaaagac acagtctgag tgtttttcac aaatatcaat caagtcattt ttccttacta
     1081 ggggttctcc tccagtataa atatacatat atacgcctag ttccttgccc tggctaataa
     1141 tatcgtcaat ttctttataa gtaagattta gtttattgcc atactctgct gcccagcagc
     1201 ccttgcaatt catattgcag gcagaggtag ggtccagtaa aattgtccag ggtatattgc
     1261 aattgtactc ctggcgcaac ttttcatgta ttggtcctcc aattaaactg gcattaaaaa
     1321 acaagtttcg ggaaagtgtt tttaatacat gctgatcgat cctctctaca ctctgatgaa
     1381 taaaatcata atagggactc tcggggtttg tcatcgcttg gcgaacccct tttctttggg
     1441 aagcaaaatc atctcctgca aatgtatcag cccaatctaa gattttctcg aaattcttct
     1501 tgggatcctt aagtgcataa tctatcattt tgttgattcc aagttttttt acagtatttg
     1561 aaatatccat gaattttgtc ctccttatta tattatcagt tatttgctaa tatgggcctt
     1621 gaaggggccc ttattttttc atagaaaaag cacttaactt cttcctcttt agtagcaaaa
     1681 caagtaaggt gctccctcac ggattttttg ctacaagttg actggaagtt tctaaatgaa
     1741 aaccacccct tttagaagaa agcttgcagc ttttctcgac tttatattca gtttgtgctt
     1801 cataattgaa attttgagca tatccttaaa cccctttaag gggctatact tatactatac
     1861 ccaagtccaa ggagctttag taggtttttt agagaattca tcctaggatg gttcttctat
     1921 ttctattata ctcaataagg gcttttttat aaagggatat gcctatctct atctatatag
     1981 ctgtcctcct aaggggcctt ttgataaatc cttctttttt tattaaaaaa cccccttatc
     2041 taataaaaga ctagagggtt ttatcctagc ttgcttacat tagggtttgc aaatggctcc
     2101 tgtggaagcg gatgtgacga tttttctgta tttttctagg tatccctttt gtttattttc
     2161 cttgggctgc cagttctttt ttctttgggc aagttcttct tctgataaat caacatgtaa
     2221 aactcccttg atcatatcaa tttcaatctt gtcgccttct tctaaaaaag caatgggtcc
     2281 ccctagggct gcctctgggg aaacatgccc aatggccgct ccccttgtgg ctcctgagaa
     2341 tctaccatcg gtaattaggg ccacatcctt atcaaaatcc attcctgcta aggttgaggt
     2401 gggagaaagc atctctctca ttcctggtcc acccctgggg ccttcatagc gaataacaat
     2461 cacatcgccc ttatgaatct tgcctgagcg gattgcctca acagcttctt cttccccttc
     2521 aaatatcctg gcgggaccac tatgtcttaa catttctttg gcaacggctg agcgcttaac
     2581 tacagctccc tcgggggcta agtttccctt gagtacagcg attcccccgc tttggctata
     2641 ggggttttcc gtggagcgaa tcactgggga gtcatttctt ttttgcccct tggtgatctc
     2701 ccctattgtt ttatccagga ctgtttgagc gctaagatcc agtaggttct ttttacttag
     2761 ctctgccata agaacagtga ttcctcccgc atagtagagg tcttcaatat gatctggacc
     2821 tgctgggctt aacctacata aattaggggt tttttcactg atctcattga tcttatctaa
     2881 attcatatct acccctgctt cataggcaat agcagtgagg tgtaagaccg tattggtaga
     2941 acagcctaga gccatatcta ctaccaaggc attgtcaaag gcagcagggg ttaagatttg
     3001 gctgggccta atatcttttt ccactagctt cataatttgc atgccagaat atttggccag
     3061 gcgcatgcgc tctccaaaaa cagcgggaat ggttccattt cctggcaggg ccatgcccag
     3121 ggcctcagtc atacaattca tggagttagc agtaaacata cctgagcaag acccacaacc
     3181 aggacaggct gcatcttcca tttcatataa ttcttcttct gatattttcc ctgcattaac
     3241 tgcgcccacc gcttcaaaca tggtgcttag atctattttt ttgcccctat gcctgcccgc
     3301 tagcatggga cccccactca caataagggc tggtatatca agtctagccg ccaccataag
     3361 catggctggc accaccttat cacaattagg aatcattacc agtccatcta ggccatgggc
     3421 cagggtcata atttcgacac tatctgcaat aagctctcga cttacaagag agtatttcat
     3481 tccctggtgg ttcatggcga ttccatcgca tacggctatg gcggggaatt caaggggggt
     3541 tcccccctcc attaacaccc ctgccttgac ggcttctgct acttgcctaa gatgtttatg
     3601 gccaggtaca atttcattaa aggtattcac aactcctatc aggggtcttc taatttcttc
     3661 tgctgtgagt cccatcgatt tgagtaagga gcgatgggga gctttttcta cacccagtgt
     3721 gactacatga cttttcatct tattttttta accaagacat catttttctt agctttttac
     3781 ctacaagctc catagggtgg ttttgttctt gtcttctttt tgccttcata aagggacgat
     3841 tggccttgtt ttctaaaatc cagtttttag caaaggtgcc atcttgtact tctgctaaga
     3901 cctttttcat ttcttttctt gtctcttctg tgatgattct ttttcccaca ctatagtcgc
     3961 catactcggc tgtatcacta atggagtagc gcatatagct aagtccgcct tcattaacca
     4021 tatcgataat gagtttcatt tcatgaacgc attcaaaata agcgctcgca gggtcatagc
     4081 ctgcttcaac taggacttca aagcccattt tcatcagctc ggttacccca ccacataaga
     4141 tggcctgttc tccaaaaagg tctgtctctg tttcctcttt aaaggtggtt tcaagtaccc
     4201 ctgcccttgc tcctccaatt ccagctgcgt aggaaagggc tagctccatg gtatcccctg
     4261 ttacatcctg gtagaccgtc gctagacaag gaacccctct accttctaaa aattggcttc
     4321 tgactgtatg gccaggtccc ttaggggcta ccataaatac attgacatcc tctggtggaa
     4381 ggatttgacc atagtgaaca ttaaagccat gggcaaaaac taaatacttt cctctggtta
     4441 gattgtcttt aatacagctt tcgtataatt ccacttgtct ttcatcacta acaagtagaa
     4501 caatgacatc ggtttctttt acagcttctg ctacggttct taccttgagt cctgcttctt
     4561 cggccttctt ccaggactta ctagcaagat aaaggcctac tgttacatct acccctgatt
     4621 cgtgtagatt gagtgcatgg gcgtggcctt gacttccgta tcctataatg gatacttttt
     4681 ttccttggat aagttctacc ttacaatcct ctgcatagta catttttgac atttgatttc
     4741 tcctttttat ttgtgttttt ttcttctttg gttgatttac attttttccc cgcgactcat
     4801 ggccaggatg ccagttttgg ctatttctag aatttcatag gatttcatca tttcgacaaa
     4861 accatctagt ttcttttcat cacctgttag taaaatagtc agtgaactac ttgatacatc
     4921 cagtacactc cccct
//
LOCUS       DASRNA010000175         5817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_491833,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000175 DASRNA010000000
VERSION     DASRNA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491833"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..455
                     /gene="yajC"
                     /locus_tag="VFD08_04860"
     CDS             201..455
                     /gene="yajC"
                     /locus_tag="VFD08_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZK10381.1"
                     /translation="MQNPLYLILLYGGMFALMYFLLIRPNKKKSQAAKEMRDNLKIGD
                     QIITIGGFNGKIVKLGDDDITVDFNGTSILIKRWAVGSVL"
     gene            458..1108
                     /gene="sfsA"
                     /locus_tag="VFD08_04865"
     CDS             458..1108
                     /gene="sfsA"
                     /locus_tag="VFD08_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HZK10382.1"
                     /translation="MIPAHFIRRNNRFVAEVEVDGNKTLVYVPTTSRLTELFFRGNKV
                     LLKDHGKGKRKYRYSIEGVLKGDYFLSINSTLPNKLFKESYQKGELPFLGLEGEVQSE
                     VRVSDKSRLDFKIGNTYIEVKGVTLEQGGISYFPGSPTTRGIKHLHELAKLSKSSRAM
                     IVYIVLVKCQAFSLNPKDKAYKETFLSLEDKVELFALAYEDFPKPIFKGELPFLLE"
     gene            1277..1771
                     /locus_tag="VFD08_04870"
     CDS             1277..1771
                     /locus_tag="VFD08_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZK10383.1"
                     /translation="MNKKAKIHPSVYVHSSAVIMGDVTLDQGANIWPGVVLRGDYNHI
                     KIGENTNIQDLACLHINTNLPCLVGKNVTVGHGAIVHACTVEDNVLIGMGAILLDDCH
                     IGKGSWIGAGSLVTEGTIIPPGVLALGTPAKVIRELTSQEKEEHLVQNAAYVKRAGVL
                     KEKE"
     gene            1775..3091
                     /gene="secD"
                     /locus_tag="VFD08_04875"
     CDS             1775..3091
                     /gene="secD"
                     /locus_tag="VFD08_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZK10384.1"
                     /translation="MNRKSQLLLIFFGLAIILLALATFTNTLGIGPLVNQLNLGLDIE
                     GGVAIVFEGNQKDMSDQEFAIAMEQTRNVLARRIDAFGLTEPNITKQGNNRIRIELPG
                     AKDVDEALAQVGKTAVLSFYQVMDGVFVNEGSPFDPISFPGNLLFQGDKLETAGVGVV
                     EGAPIVSLKLTKEATQFFTDASREIVDNYSESRGQIAIILDDRVISAPFIDSYLDSED
                     LIIRGNFTSDEVIMLSSLIRGGALPLDLEEVQTTFINATLGRTALERSIKAGIYGLIS
                     VLLIMLVRYRYAGLLASMSLLLYGALLFIVMILFNATLTLPGIAGLVLSVGMAVDANV
                     IIFERLREEMNLGKSLRGAINASFSKAMTTILDSNMTTLLAAVILYNFGEGPIKGFAV
                     TLMIGIVLSLFTAVVVTRVLINLSSQSTMLQNPALYAVKGGQDENH"
     gene            3078..3932
                     /gene="secF"
                     /locus_tag="VFD08_04880"
     CDS             3078..3932
                     /gene="secF"
                     /locus_tag="VFD08_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZK10385.1"
                     /translation="MKIIKHYKKYFILSAALILIGLGVIAFGGLNYGIDFTGGTMLTI
                     PTGESLAVDDISNVLADFDLDPVIQHQGADDATIIIKTKKPLDTEQRNAIRDKLIEDF
                     DLSADTKMDGEQFGPSIGEEISKKALISMGLAAAGMLVYITVRFKFFYGVAAIIALIH
                     DLLILIGLYAVFNITVNSNFIAAVLTILGYSINDTIVIFDRIRENSKSMRNKPKLEIA
                     GHSLDQSLSRTFLTSITTFVVVFFLYIFGVPTVKEFALPLMFGIVVGTYSSVFLAGPI
                     WALLSKDK"
     gene            3932..>5817
                     /locus_tag="VFD08_04885"
     CDS             3932..>5817
                     /locus_tag="VFD08_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZK10386.1"
                     /translation="MLPSLYYDNRWKAVDYDEKSLELAKELGLSYFLTAHLGRFYKNE
                     KEIKEFLWPKPSQNQIPGTEEIAKRIKVALDQKKRILFFGDYDVDGLSSLALMVLFLR
                     ENGFDPLHYVPSRYDEGYGLTLKAIEKLPEVDLLVSFDCGITAVEEVEVLKNRGVEVL
                     ITDHHQPGESLPDCLIMNPQLGEEFKELAGVGVAYKLATYLSQTYGYALPKDSIVFAM
                     LGTVCDLMPLIKENRLLVKEGLKAYEKTSHPGLLALRKVAPGALDAQHIGFGIGPMIN
                     AAGRLGEAEEALKLLLAEGDSFDLALRLRDYNNQRKEEEKQVVKEALKKTDQNQPLLI
                     VSGDWKQGLLGLAASRISQRYRKPAIVLDKNLKGSSRSVGNFSIIHALDYSAKYLDHY
                     GGHKMAAGLAISEGKYEEFKEKVFSYTRENFDFLQTRKCYDYLCVEPGDINLELLDKL
                     NFLSPYGIKNPQLVFQLKNLSFVECIELGKSGRAFTLIFKAKDRRFEFICFDPQLISL
                     LEKEKYDLIFTASEDVFRGILSLKLQLRDLRPSKPSLEKSRLFLPFYEGLAKSIERYK
                     AKKQEFSFPKFEEDRIYKLGDPEFPESFPHPKSFTGEESSLLEDLPSREDLVKIYSWL
                     RRQG"
BASE COUNT         1783 a          998 c         1248 g         1788 t
ORIGIN      
        1 gaacctatca taagaaagaa aaactaagag accataaata aaaaaataac tttattaaag
       61 tttcatatat ttttttgcaa tatttttcaa atctctagct atggaattat tattttttct
      121 tccatgggat aaaaggggta aaatgattgc ataggacgat tagatatagt actataagag
      181 ggatatgaaa ggagtaaatt atgcaaaatc cactatattt gattcttctc tatggcggta
      241 tgttcgcctt gatgtatttt ctattgatcc gccccaataa aaagaaaagc caggcggcaa
      301 aggaaatgag agacaatctt aaaataggtg accaaattat aaccattggc ggctttaacg
      361 ggaaaattgt aaagctgggt gatgatgaca ttaccgtaga ctttaatggt acatctattt
      421 taattaagag atgggcggta ggatcagtcc tataataatg atccctgccc actttattcg
      481 aagaaataac cgctttgtgg cggaggttga agtcgatgga aacaagacct tagtttatgt
      541 gcccacgacc tctagactga cagaactatt ttttagagga aataaggtct tattaaaaga
      601 tcatggtaaa ggaaaaagaa aatatcgtta ttctattgaa ggcgttctta agggagatta
      661 ttttcttagt attaattcca cactaccaaa taaactcttt aaagaaagct atcaaaaggg
      721 ggagttaccc tttttgggcc tagaaggaga agtccagagt gaggttagag taagcgacaa
      781 atcccgcttg gattttaaga ttggaaatac ctatatagaa gttaagggag ttacccttga
      841 gcaggggggc atttcttatt ttcctggctc tccaactact agaggcataa agcatctaca
      901 cgagctagcc aagctttcta agagtagccg ggcgatgatt gtatatattg tgctggttaa
      961 gtgccaagcc tttagcttga atccaaaaga caaggcctat aaagagacct ttttatccct
     1021 tgaggataaa gtggagctgt ttgccctggc ttatgaggat tttccaaaac ccatttttaa
     1081 aggtgaattg ccttttttac ttgagtaaag gttaaaaaac tctagaggat aaaagtatgt
     1141 aagcataaaa gatagtagag tcatttatag gcttactgca gaaagaccat ggggtaaaat
     1201 ttaagggtct ggcaagatgc agctacggct gcattttatt tttaaaaaga aaatactaaa
     1261 taaagaaagg aatacgatga ataagaaagc aaaaatacac ccttctgtat atgtacattc
     1321 ctcggctgtt attatgggtg atgtgacttt agaccaaggt gccaatattt ggccaggagt
     1381 tgtactaaga ggagattata atcatataaa aataggggaa aacacaaaca ttcaagacct
     1441 tgcatgtttg catattaata ccaatcttcc ctgccttgta ggaaaaaatg tcacagtagg
     1501 tcatggagcc attgtgcatg cttgtacagt ggaagataat gtacttatag gcatgggagc
     1561 tattcttctt gatgattgtc atattggaaa aggctcttgg attggtgcag gaagtttggt
     1621 aacagaagga accattatcc ctccgggagt tttagctcta ggaacaccag ccaaagtaat
     1681 tagagaactg acttcccaag aaaaagaaga acaccttgtg caaaatgctg cctatgttaa
     1741 gcgtgcaggg gtgttgaagg aaaaggagta aactatgaat agaaaaagtc agttgctttt
     1801 gatttttttt ggccttgcta tcatcctttt agctttggcg acctttacaa ataccctagg
     1861 tataggaccc ctggtaaatc agctgaatct gggtcttgat atcgaaggtg gtgtggccat
     1921 tgtttttgaa ggcaaccaga aagatatgag cgaccaagag tttgccatag ctatggagca
     1981 aaccagaaac gttctagccc ggcgcattga cgcctttggt ttaacagaac caaatattac
     2041 caagcaggga aataatcgaa ttcgaattga gcttcccgga gctaaagatg tggatgaggc
     2101 cctggcccaa gtaggtaaaa ctgctgtttt gagcttttat caagtgatgg acggtgtttt
     2161 tgttaatgaa ggatctccct ttgatccaat atcttttcca ggaaaccttc tctttcaagg
     2221 agataaactt gaaacagcag gtgttggggt tgttgagggc gcaccgattg ttagtttaaa
     2281 actcaccaaa gaagctaccc agttctttac agatgcttca agagaaattg ttgataatta
     2341 tagtgaatcc cgaggacaaa ttgctattat tttggatgat cgtgtcatta gtgctccctt
     2401 tattgattca tatttggact ctgaagatct tattataagg ggaaacttta cttctgatga
     2461 ggttattatg ctgtcttctt tgattcgagg aggagccttg ccccttgatc ttgaagaagt
     2521 acaaactaca tttataaacg ctactttagg aagaacagcg ctagaacgta gtattaaagc
     2581 tggaatctat ggacttattt ctgttttgct tattatgctt gtgcgttacc gctatgcggg
     2641 gcttcttgcc tccatgtcgc tactgcttta tggcgctttg ctatttattg taatgatctt
     2701 atttaatgct actttaactc ttccggggat tgcaggtctt gtgctgagtg ttggcatggc
     2761 tgtggatgcc aatgtaatta tttttgagcg tcttcgtgaa gaaatgaatc ttggaaagag
     2821 tttgagggga gcaataaacg cctccttttc taaagcaatg acaactattt tggactcaaa
     2881 tatgactacg cttttagcgg ccgttatcct atataacttt ggagaaggtc ccattaaagg
     2941 gtttgctgtt actttgatga ttggtattgt gctgagcctt tttaccgcag tagtagttac
     3001 aagagtttta atcaatcttt ccagccagag caccatgctg caaaatccag ctctttatgc
     3061 tgtgaaggga ggacaagatg aaaatcatta aacactataa aaaatacttt atactttcag
     3121 ctgccttgat tcttataggt cttggagtga ttgcttttgg aggactcaat tacggtatag
     3181 actttaccgg aggaaccatg cttaccattc caacgggtga gagtcttgca gtggatgata
     3241 taagtaatgt ccttgcagat tttgatcttg accctgtcat tcaacaccaa ggggcagatg
     3301 atgcgaccat tattattaag acaaaaaaac cactagatac ggagcagaga aatgccatta
     3361 gagataagtt aattgaagat tttgacttgt ctgctgatac taaaatggac ggcgaacaat
     3421 tcggtcctag tattggagag gaaataagca aaaaagctct tatttctatg ggtcttgctg
     3481 ctgcaggcat gctagtttac attaccgttc gctttaagtt tttctacggg gtagctgcta
     3541 tcatagctct tatacacgac cttttgattt tgattggtct ttatgccgtt tttaatatca
     3601 ctgtcaatag taattttatt gcagctgtgt taaccattct tggttactca atcaatgata
     3661 cgatagttat ttttgaccga atccgagaaa attctaaatc tatgagaaac aaaccaaaac
     3721 tagagattgc aggtcatagt ttggaccaat ctctttcaag aactttttta acctccatta
     3781 ccacctttgt agttgttttt ttcctctata tttttggggt acctacggtt aaagagtttg
     3841 ctctaccact tatgtttggc atcgttgttg gaacctattc ttctgtcttt ttagccggtc
     3901 ctatttgggc tttgttaagt aaggataaat agtgctacca agtctttatt atgataatcg
     3961 ttggaaggct gtggactatg atgagaaaag cctagaactt gcaaaagaac tagggctttc
     4021 ttattttctt acagcccatc tgggcaggtt ttataaaaac gaaaaggaaa taaaagaatt
     4081 tctttggccc aagcccagtc aaaatcagat tccaggaact gaggagattg ccaagagaat
     4141 aaaagttgct ttagaccaaa agaaacgaat tctatttttt ggagactatg atgtagatgg
     4201 tcttagttca cttgctttga tggtgctttt tcttcgagaa aacggttttg atccactaca
     4261 ttatgtccca agccgttatg atgaaggtta tggtcttacc ctaaaggcca ttgaaaaact
     4321 acctgaagtg gaccttcttg ttagctttga ctgtggtatt actgcagtag aagaagtgga
     4381 agtcttaaaa aatagagggg ttgaggttct aattacagac caccatcagc caggggaaag
     4441 cctgccagac tgccttatta tgaatcctca actaggggag gaatttaagg aactagctgg
     4501 ggtaggagtg gcctataaac tggctactta tttaagccag acttatggct acgcactgcc
     4561 aaaagatagt attgtttttg ctatgctagg cacggtttgt gatcttatgc ccctaataaa
     4621 agaaaatcgt ttgcttgtaa aagaaggact taaggcctat gaaaaaactt ctcaccctgg
     4681 acttttggcc cttcgaaaag tggctcctgg ggcccttgat gcccaacata ttggttttgg
     4741 cattggcccc atgattaatg ctgctggccg cttaggggag gcagaagaag ccctaaagct
     4801 acttctagca gaaggtgact cttttgattt ggcccttaga cttcgcgact ataataacca
     4861 gagaaaagaa gaagaaaagc aggttgttaa agaggccctt aaaaaaaccg atcaaaatca
     4921 gcccctactt attgtctcag gagattggaa acaaggactt ttaggtctag ctgcttctcg
     4981 tatttcccag cgctatagaa aacctgccat tgtactagat aagaacctta agggttcatc
     5041 aagaagtgtg ggaaactttt ccattatcca tgcccttgat tatagtgcca aataccttga
     5101 tcactatggt ggtcataaga tggcagcagg tcttgctatt tctgagggaa agtatgaaga
     5161 gttcaaagaa aaggtatttt cttataccag agaaaacttt gattttttac aaacaagaaa
     5221 atgctatgat tatctttgtg tagagccagg ggacattaat ttagagctat tagataagct
     5281 gaacttttta tctccctatg ggataaaaaa cccccagctt gtttttcaat taaaaaacct
     5341 atcctttgtt gaatgtatag aactaggtaa gtcaggtagg gcctttactt tgatatttaa
     5401 agccaaagat aggcgttttg aatttatatg ttttgatcct caactgattt ctcttttaga
     5461 aaaagaaaag tatgatctaa tctttactgc cagcgaagat gtttttagag gtattttaag
     5521 cttaaagctt cagctaagag accttagacc tagtaagcct agtttggaaa aaagccggct
     5581 ttttttacct ttttatgagg gcctagcaaa gtctattgag cgatataagg caaaaaaaca
     5641 ggaattttct tttccaaaat ttgaagaaga ccggatctat aagcttggcg atcctgaatt
     5701 cccagaaagt tttcctcatc caaagagttt tacaggagaa gaaagttctt tacttgaaga
     5761 tctacctagc agggaagatt tagtaaaaat atacagctgg cttagaaggc aaggaaa
//
LOCUS       DASRNA010000176        14903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1101059,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000176 DASRNA010000000
VERSION     DASRNA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14903
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101059"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFD08_04890"
     CDS             <1..298
                     /locus_tag="VFD08_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZK10387.1"
                     /translation="IHREYKEEEVFTLLVLDATTGQNALNQAEFFKEYAHIDGLILSK
                     MDGSSKGGFVFAIKDRLDLPVVFMTLGEKLEDIAEFDPVYFVKGIFDEEEEEEK"
     gene            complement(321..1550)
                     /locus_tag="VFD08_04895"
     CDS             complement(321..1550)
                     /locus_tag="VFD08_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZK10388.1"
                     /translation="MFFHVDVNSAYLSWEASYRLMQGESLDLRSIPSAIGGNPRERKG
                     IILAKSIPAKAYGIQTGETLVSALGKCPSLTIVAPNFSLYKKCSRALSQLLESYSPKV
                     QAFSIDESFMDMGSIDDPLALAEKLREEIEETLGFTVNIGLGPNKLLAKMASDFKKPN
                     RVHSLFYEEIPHKMWPLPVGELFMVGKKTREKLLLMGIETIGDLASANPDLLYSRMKS
                     HGLLIHKYAQGKEFSPIESYSPFEAKGVGNSTTLPRDINDAELIELYLLGISEMVSFR
                     LRSIDKMAYVLSLHYRDNNFKTRRVQRKLLAPLDATSSIYKQAKILFYELWKEEPLRQ
                     LGLSLSELVGNQYYEQSFLDQDQEKLRKVDATIDNLRKKYGRQIIQRASFLYTPVDPM
                     IGGVGEDKDELILSSKL"
     gene            1668..2618
                     /locus_tag="VFD08_04900"
     CDS             1668..2618
                     /locus_tag="VFD08_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZK10389.1"
                     /translation="MEKIYEVLRVLEGKPVFLKEHLDRLQNSWLFYHSEPMDSKTLKK
                     EILALASQGLDPHNIRIEVEIEGGAYLLTRVEGAYPSQEMKEKGVDLATYKHMRHNPQ
                     VKAIDPLLNERAQAFRQEKGVYSLLYVYEGEVGECQRANIFFIRDGVLITARDEDVLL
                     GVTRQKVLEIAQDLNLPLVKRIVRQEELFTMEAAFMTGTSIHILPVRKIDGFAYNVEH
                     PFLEKLSREMDRQFYEEERVVESEDTQEEKDFERGGQMKKLYRSDDAKLAGVCGGLGE
                     YLQVDPNVIRILWIIFAAMGGSGILAYLLAALIIPKRNES"
     gene            2621..3019
                     /locus_tag="VFD08_04905"
     CDS             2621..3019
                     /locus_tag="VFD08_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZK10390.1"
                     /translation="MKKLYRSRNDSMLTGVCGGLGEYFNIDATLIRLAFALFFFTGIG
                     IFVYFIAAIILPKAPLAEGESPYKKDEVDGVESSNTSFALGVLLVFIGLALLIKRYIL
                     PLMPHYMKGLIWPLVFIGLGAYLVWGRKES"
     gene            3019..4491
                     /locus_tag="VFD08_04910"
     CDS             3019..4491
                     /locus_tag="VFD08_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HZK10391.1"
                     /translation="MDLKKLTILHNNDIHGDFFAKEEQEAVFGGMSMLSGYVQKVRRE
                     EKNVVYAIAGDMLQGSIIDQEYRGVSTIDIMNIIDPDIVTLGNHEIDYGLAHLMFLER
                     CARFPIINANLHIKPTNTRLFNSHKIMMVGGIRILFIGIITEDILSYAGQDPILSSFV
                     DVKEAAREVEYICNNYKDIDIDFTVLLTHIGFDKDVELAGLLPKEIGVDLILGGHSHT
                     MMEEPAEVNDILIAQVGTGSTHIGRFDIIIDMDRNAVHEYSWEAIPIDDSHCPRDRIM
                     DEVLASYSEKVDNKYNQILTRLKKPLEHPDRYRETSMGNFFADLFKSQLDVDLFILGS
                     GSIRGARLEDVITLSKLREIFPYDEGIISFSVDGQRLEDMIFTFFDNIYAGKTHEFYQ
                     FSRDFSVKVKGEGKVEQMLLSGKPLEKSRKYRIAMQKFHYGVLENNFGINNEGLKVYG
                     PARSVSTSSFDIIDEYMQKHHLVSAKVEGRIFLEVPLKID"
     gene            4744..7374
                     /locus_tag="VFD08_04915"
     CDS             4744..7374
                     /locus_tag="VFD08_04915"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZK10392.1"
                     /translation="MKKHKMEKTYDPQEFEDRIYKMWMESGDFEAEVNPDKKPFTIVL
                     PPPNITGSLHEGHALNHTMQDILIRYKRMKGFEALWLPGTDHASIATEVKILDKIRKE
                     EGRGKEDLTREEFLDKAWLWKEKYGDTIVNQMKKLGNSCDWRRERFTMDEGCSRAVTE
                     VFIRLYEEGLIYRGTRLINWCSDCGTSLSDVEVEHEEVEGAFYHINYPIEGSDKKLVI
                     ATTRPETILGDTAIAVHPEDSRYKELVGKYALLPLLGRRLPIIADEYVDRELGTGALK
                     ITPAHDPNDYEIGKRHKLASINIFTPGAKINEEGGDYVGLDRFAARKKIVEDLKDKGY
                     LVEIKDHLHSVGHCYRCHQPIEPRESLQWFVQMDSMAKMALESVEKGEMEFVPERFVK
                     TYQHWLENIKDWCISRQLWWGHRIPAYYCLDCGEMMVSRQEVTSCTKCAGTKIEQDED
                     VLDTWFSSALWPFSTLGWPDKTPEMEYFYPTDVLITGYDIIFFWVVRMMFSGLKHIGI
                     APFHHTLINGIVRDAQGRKISKSLDNGTDPIVIIDEYGADALRFMLISGTSVGSDTRF
                     LIEKVEAARNFANKLWNASRFLLTDLEMKAYSIEEKQLDLSDKWILSRLSQTTQAVER
                     SLERYELGQAADRVLDFVWSDYCDWFIELSKEALYSGDEQRKERKQAVLLFVLEDILK
                     LLHPFMPFITEEIWQHLPQRTSHLICSAWPLAGKTYPEEENLMALLQEAISRIRNIRA
                     EMDIPPSKKGRLFLQGPRAEEVLTYAGYFRAMASSDEISLAEEQVADASAILLDGLSL
                     YLPLDELIDHEKELKRLEKEENKLDSEIKRSRGKLNNAGFVAKAPAELVKKEEEKLES
                     FLRLQEELEEKIILAKEKIQ"
     gene            7371..8663
                     /locus_tag="VFD08_04920"
     CDS             7371..8663
                     /locus_tag="VFD08_04920"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZK10393.1"
                     /translation="MKIQEALNFLNQSYKLGRKLGLDNIKRLLLELGSPEKKMKVIHL
                     AGTNGKGSTSAMIYHVILEGGYKVGFFSSPHLVHYNERFQYNGKTISDTELAKSIEKV
                     KFACERLLAQGYDHPTEFEILTAVGFVFFADKGLDYAVIEVGLGGRLDATNSVEEPLV
                     SVITPIAMDHESYLGDNLLAIAGEKAGIIKPQIPLVSGLQQKEVLDLLKDKARKKGSP
                     FYPSDPSQLEIIRSDLLENIFRYKGREYKLKLLGRHQIDNAILALETLYLLREKGQID
                     LEEEQIRRGLLETRWPGRLEKISQKPDIFIDGGHNAHGVRAVAQNLLLDEKNRRLLLL
                     GMRLDKDYKEVLSLLLPLFREVVLTEPLGDQVVLVEDLQKEAALRGVMAEGDRDYKKA
                     LSLVLAKANEEDQIFVMGSFYLIGQVKEEVLRRIGVEK"
     gene            8660..9235
                     /locus_tag="VFD08_04925"
     CDS             8660..9235
                     /locus_tag="VFD08_04925"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZK10394.1"
                     /translation="MIVLISGSPRRRELLSRITSKVIYDVSHVQEPVGEKDPQKLVIK
                     SARAKLKDRLHVHKKGILLACDTIVYADKVLGKPKNREEASQMLAFLSGRSHQVYTSL
                     VLHDKNKGRLVEEVVCTKVYFSSLEEKEIQAYLDTGEYVDKAGGYGIQGFGSLLVEKI
                     EGDYFNVMGLPLNRLYLILKKEFSLDLLRDI"
     gene            9314..9994
                     /gene="radC"
                     /locus_tag="VFD08_04930"
     CDS             9314..9994
                     /gene="radC"
                     /locus_tag="VFD08_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZK10395.1"
                     /translation="MNIPIRQLPSWERPREKILEKGVESLSNAEVLAVLLGSGNSKQS
                     ALLLAQELLTSLDKGLYQLEEITIYELMDFPGIGPAKACRILCGLELARRVKAAPAKP
                     LNVSSPGAVYSYMLSKMQNYQKEVFVCLHLDSKNNIISEDIVSIGTLNRSLVHPREVF
                     KKAVKNGAASILVVHNHPSGDPNPSQEDKQVTSRLKEVGELLGIPLLDHLIVARDSRF
                     SFREAGIF"
     gene            10047..11855
                     /locus_tag="VFD08_04935"
     CDS             10047..11855
                     /locus_tag="VFD08_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HZK10396.1"
                     /translation="MKLEQLLKQVEKPTRYLGEERGSVIKNPDEVKADFLLAFPDLYE
                     VGMSHMGLHILYGLLNEKEDFLCERLFAPAADMEKLIREGKTPLFSIENKRPMKDFDM
                     IGFTLQYEMSFTTILGMLELGGLALRSKDRGPEDPLIIAGGPCAFNPEPMSAFIDLFV
                     LGDGEEVLLELMELWLKTGKKDKFLKEALKIRGVYVPKYYEPEFSGGLYKGLAPLIEE
                     APLTIQKALINDLDHCYYPKKMIVPYMDIVHDRAVMEIFRGCTKGCRFCQAGIIYRPL
                     RERSVQTLVKAIDSLLTEGGYDEVGLSSLSTLDYSGILPLVEELTQTYSEDHIRLSLP
                     SLRLDTFSIDVLRLVQKRRKGSLTFAPEAGSQRMRDIINKNVNEKDLFSTMEEIFKEG
                     YKKVKLYFIMGLPGENMEDVEAIAELAYKVVDLYKEVTGKRNVEITISTSCLVPKPFT
                     PFQWMAQEKVEDFEEKQRYLRGLLRGKSFRYNYHDAKTSVVEGRICRGGRKMADVLEL
                     AYKKGAFLEAWMENFDETIWQEAFKEMGMDFDQELSFSLEETLPWDHIDVGVKKEFLK
                     KEWMAAQKGQLTPDCRQGCLGCGVNLESFGGVCYGN"
     gene            11845..12462
                     /locus_tag="VFD08_04940"
     CDS             11845..12462
                     /locus_tag="VFD08_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HZK10397.1"
                     /translation="MVIRLWMIKEGPMMFLSHLELMRTLERCFKSARLPISYSQGFNP
                     RPLMNFAFPLAVGVEADKLLLEIELKEGADPSLLEGVDFPRGLELQDYKVVEKGPSLM
                     SLVISARYHIRGDKKKLQKIKTEDSLPFLKRNKRGRYNKKEAREFILSYKEKEDGYDF
                     LLKAGSRANLKATDLLMALLEKEEEVHAYNVRLLDVYDDKGKSLW"
     gene            12456..13361
                     /locus_tag="VFD08_04945"
     CDS             12456..13361
                     /locus_tag="VFD08_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10398.1"
                     /translation="MVISYETDSFRRRFIFEGENLIQGRSFLKEGERGEVYRGKVLKR
                     VGNLGYFVDLGPFKGLCQEKLNSGDIQLFEIIKRQVDKEPLLTSKVGFMGESLILQDG
                     PGIYYSKSIPSSYRRLLEELKLEGVYFRREASRLPLHLVKKEYDSLRAKKKALQNKNQ
                     LGLVYSPPFKEEGDPFYFMELEEKILHLRRGKSKKDGITLFFEPTRAGLVIDVNGVGK
                     EEDINRKAGEMIEESLILMNVGGLVIIDLLGRGRGYRGEGSLTKEGIFVKSLPVRGAN
                     LFYVPLEKLKNDYKKLRNKLSEEYE"
     gene            13358..14014
                     /gene="sdaAB"
                     /locus_tag="VFD08_04950"
     CDS             13358..14014
                     /gene="sdaAB"
                     /locus_tag="VFD08_04950"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HZK10399.1"
                     /translation="MKEVNLFDILGPVMIGPSSSHTAGAVRLGNIAAMIYNKKITRVD
                     FYLHGSFANTYKGHGTDLALVAGILGLKTYDEKIVDAFSLADEEGLAFSFYRKDLGYV
                     HPNTVRIVFDENFSVTASSLGGGRVEIIDINGTEVSLQGDYPTLVLQYEDKLGIIQEV
                     SALIAATGVNIAAMKVTRQRKHATMVLELDGKINKETFESLKVLGLNYLASVGAVEEK
                     "
     gene            14011..14883
                     /gene="sdaAA"
                     /locus_tag="VFD08_04955"
     CDS             14011..14883
                     /gene="sdaAA"
                     /locus_tag="VFD08_04955"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HZK10400.1"
                     /translation="MIYKAADILKKCEEKNCSIAELTRQDEAATASISVEELDLKMKE
                     ILEIMETSSSLGLKEEIFSLSGMTGASAKKLWDNRDQTSLSGEFILSAMARALSTSEV
                     NSSMGRIVAAPTAGASGILPSALMSAKEKLSLGGEELILGLYTAGAVGKIVAQSATIS
                     GADGGCQAECGTGAAMAAAALVELMGGSPQLAFHGASFALIHIMGLVCDPIAGFVEFP
                     CSLRNASGIVNAFISADLAMAGLSSPIPFDEVVDAMYQVGKMLPEALRETALGGIAAT
                     PSAKALEEKYLGKG"
BASE COUNT         4543 a         2723 c         3486 g         4151 t
ORIGIN      
        1 tattcataga gaatataaag aagaagaagt ttttaccctt cttgttcttg atgccactac
       61 gggacaaaat gctcttaatc aagcggaatt ctttaaagag tatgcccata tagatggact
      121 aatcctttca aagatggatg gctctagcaa gggaggattt gtttttgcca taaaagaccg
      181 gcttgatctg cccgtggtct ttatgaccct gggagagaag ctagaggata ttgcagaatt
      241 tgatccggtt tactttgtta aaggcatttt tgacgaagaa gaggaagagg aaaaataacc
      301 ccttttgaat aaggggtttt ttatagctta ctggagagga taagctcatc tttatcttct
      361 cccactcctc caatcatagg atctacggga gtgtagagaa agctagccct ttgaataatt
      421 tgtctgccat atttttttct taaattgtca atggtagcat ctacctttct aagtttttct
      481 tggtcttggt ccaaaaagct ctgttcatag tattgatttc ccaccagttc acttagagat
      541 aggccaagct ggcgcaaggg ttcttctttc caaagttcat aaaaaaggat ttttgcttgt
      601 ttataaatag aagaagtagc atctagggga gcaagaagct ttcgctggac tcttctagtc
      661 ttaaaattat tgtccctgta atgaaggctg agaacatagg ccattttatc aatggacctt
      721 aggcgaaagg agaccatttc actgatgcca agaagataaa gttcaatcag ctctgcatca
      781 tttatatctc ggggtagggt ggtggaattt cccactccct tggcctcaaa ggggctgtag
      841 ctttcaatgg gagaaaactc ttttccctgg gcatacttat gaataagcag tccatgactt
      901 ttcatcctgc tatagagcaa atcaggattt gcactagcta aatcacctat ggtttcaatg
      961 cccataagaa gaagtttctc cctggttttt ttccccacca taaaaagctc ccccacaggt
     1021 aggggccaca ttttatgggg aatttcttca taaaaaagac tatggactcg attgggcttt
     1081 ttaaaatccg aagccatttt ggccagaagc ttattgggcc ccaggccaat attaacagta
     1141 aagccaaggg tttcttctat ttcttctctg agcttttctg ccagggccag ggggtcatcg
     1201 attgacccca tatccataaa ggactcatct atactaaagg cttgtacctt ggggctatag
     1261 ctttcaagca gctggcttag ggcacgagag cattttttgt atagggaaaa attaggggct
     1321 acgatagtaa gagaagggca ttttcccagg gcagaaacga gggtttcacc tgtttgaatc
     1381 ccataggcct ttgctgggat gctttttgcc agaataattc cctttctttc cctgggattt
     1441 ccgccaatgg cagagggtat gctacgtaga tccaagcttt ctccttgcat aagccggtag
     1501 ctggcttccc agcttaaata agcactattc acatctacat gaaaaaacat ataacccctt
     1561 tagcaaatca taacagaaca gatgttcgac ttcaaggcta tactagtaaa aaaacgaata
     1621 tttcctatat aaagtggtat acttagaaga agcaaatgga ggtatctatg gaaaaaattt
     1681 atgaagtttt acgtgtttta gagggaaaac ctgttttttt aaaagaacat ttagataggc
     1741 tgcaaaattc ttggctattt tatcatagtg agcctatgga tagtaagacc ctaaaaaaag
     1801 aaatactagc gctagcctca caggggctgg accctcataa tatccgtatt gaagtggaaa
     1861 ttgagggggg agcatatctt cttacaagag tagaaggagc ttatcccagc caagagatga
     1921 aagaaaaggg ggtggaccta gccacctata aacatatgcg gcataatccc caagttaagg
     1981 ccatagaccc cctcttaaat gagcgagccc aggcttttcg tcaagaaaag ggagtatatt
     2041 ctcttcttta tgtctatgaa ggagaagttg gcgaatgcca acgagccaat attttcttta
     2101 tacgagatgg tgtcctaatc actgctaggg atgaggatgt acttttggga gtgactaggc
     2161 aaaaggtgct agaaattgcc caagatttaa atctacccct agtaaaaaga atagttaggc
     2221 aggaagaact ttttaccatg gaggcggctt ttatgactgg gacctccatt catatcttgc
     2281 ccgtaagaaa aattgatggt tttgcctata atgtagagca ccctttttta gagaaactat
     2341 caagggaaat ggaccgccag ttctatgaag aagaaagagt agtagagagt gaagatacac
     2401 aggaagaaaa agactttgaa agaggaggcc aaatgaaaaa actttatcga agtgatgatg
     2461 ccaagctagc aggagtctgt ggaggccttg gtgaatattt gcaggttgac cccaatgtta
     2521 ttcggatttt atggattatt tttgctgcca tgggaggttc aggaatatta gcctaccttc
     2581 ttgctgccct tattattccc aaaagaaatg agagctaaat atgaagaaat tataccgttc
     2641 tagaaatgat tccatgttaa caggtgtctg cggaggctta ggggagtatt ttaacattga
     2701 tgccaccctc attcgactag cctttgcctt gtttttcttt acgggaatag ggatttttgt
     2761 ttattttatt gcagcgatta ttcttcccaa ggcaccccta gcagaaggag agtctcctta
     2821 taaaaaagat gaggtagacg gggtagaaag tagtaatact tcctttgccc tgggtgtttt
     2881 acttgtcttt attggtctag ctcttttaat taaacgatat attttgcctt taatgcctca
     2941 ttatatgaaa ggtttgattt ggcctctagt atttattgga cttggtgcct atttagtttg
     3001 gggtagaaag gagtcatagt ggaccttaaa aagctaacca tccttcataa taatgatatc
     3061 catggtgatt tttttgcaaa agaagaacaa gaagctgttt ttgggggcat gtccatgctc
     3121 tcgggctatg tgcaaaaagt tcgccgggaa gaaaaaaatg ttgtttatgc cattgccgga
     3181 gatatgctcc agggctctat tattgatcaa gaataccgag gggtctctac catagatatt
     3241 atgaatatca ttgaccctga tatagtgacc ctgggaaatc atgaaataga ctatgggcta
     3301 gcccatctta tgtttttgga gcgctgtgct cgttttccta ttattaatgc caatcttcac
     3361 atcaaaccaa caaataccag gctatttaac agccataaga ttatgatggt aggaggaatc
     3421 cgaatccttt ttataggaat tattacagaa gatatcctaa gttatgctgg acaggatcct
     3481 attttaagtt cctttgttga tgtaaaagaa gccgctaggg aggtcgagta tatttgcaac
     3541 aactataaag acattgatat tgattttacc gttcttttga cccatattgg ttttgataag
     3601 gatgtggaac tggcagggct tctacctaaa gagattgggg ttgatttaat cctaggggga
     3661 cactcccata ccatgatgga agagcccgca gaagtaaatg atattctaat agcccaggtg
     3721 ggtactggca gtactcatat aggacgattt gatattatta tagatatgga tcgcaatgca
     3781 gttcatgaat atagctggga agccatcccc atcgatgata gccattgtcc tagagatcgc
     3841 attatggatg aggttttggc cagttactct gagaaggttg ataataaata caatcagatc
     3901 ctaacaaggc tgaaaaaacc cttagaacat ccagatcgtt atagggaaac ttccatgggg
     3961 aacttctttg cagatctatt taaatctcag cttgatgtgg acctttttat tctggggtct
     4021 ggctctatta gaggggcaag gttagaagat gtgattactc tttctaaatt aagagaaatt
     4081 ttcccctatg atgaagggat aataagcttt agtgtagatg ggcaaaggtt ggaggatatg
     4141 atttttacct tttttgacaa tatctatgca ggtaaaaccc atgagtttta ccaatttagt
     4201 cgagattttt ctgttaaagt aaaaggagag ggcaaggtgg aacaaatgct tttgtctgga
     4261 aaacccttgg aaaaatcgcg taaatataga atagccatgc aaaaattcca ctatggcgtc
     4321 ttagaaaata actttggaat taataatgaa ggactcaagg tttatggtcc agcgcgaagt
     4381 gttagtacct caagttttga tatcattgat gaatatatgc aaaagcatca tttggtcagc
     4441 gccaaggtag aaggtcgtat tttccttgag gttccgctta aaatcgattg acaaatagga
     4501 tgaaaacgca tattatcaat atgtagatct ccaatagagg aggagtagct ttgcaattgg
     4561 tcttagagag aaggtgtttg gtgcaaacct tcccaatgca aagacgaagg tcgcctttta
     4621 gatctgcggg gaaagcccct taacgctttt aaagtgccta aaaggcagaa atagggtggt
     4681 accacggttt gacgtcccta gcgtcgagcc gtttttttta taagaataag ccaggaggct
     4741 ataatgaaaa aacacaagat ggaaaaaacc tatgatcctc aggagtttga ggatagaatc
     4801 tacaagatgt ggatggaaag tggcgatttc gaagccgaag taaatccaga taaaaaaccc
     4861 tttaccattg tattgccacc gccaaatatt acgggcagtc ttcatgaagg acatgctcta
     4921 aatcacacca tgcaggacat tcttatccgc tataagcgca tgaaaggatt tgaagcccta
     4981 tggcttccgg gaaccgacca tgcttccatt gctacagaag taaagatttt agacaaaatc
     5041 cgaaaggaag aaggtcgcgg taaggaggat ctaaccaggg aagaatttct tgataaggcc
     5101 tggctgtgga aagaaaaata tggtgatacc attgtcaatc aaatgaaaaa actcggcaat
     5161 agttgtgatt ggcgcagaga gcgctttacc atggatgaag gttgctctag ggctgttaca
     5221 gaggttttta tacgcctgta tgaagaaggc cttatttatc gaggaacaag actgatcaat
     5281 tggtgtagcg attgtgggac ctcccttagc gatgttgaag tagaacacga ggaggtagag
     5341 ggagcctttt atcatataaa ctatcccatt gaaggcagtg ataaaaagct tgtgattgca
     5401 accacaagac ccgaaacaat cctaggagat acagccattg ctgtccatcc agaagactcc
     5461 cgttataagg aactagtggg aaaatatgcc cttcttcctc tacttggaag aagactgcct
     5521 attatagccg atgaatatgt ggatagggag cttggcacgg gggcattaaa gataacacct
     5581 gcccatgacc ccaatgacta tgagattgga aaacggcaca agctagcttc tattaacatc
     5641 tttacccctg gagccaagat caacgaagaa ggcggagatt atgttggact agaccgtttt
     5701 gctgcccgaa aaaagatagt agaagatcta aaagacaagg gatatcttgt agaaatcaaa
     5761 gaccatcttc actctgtggg tcattgttac cgctgccatc agcccattga acccagagag
     5821 agtctacagt ggtttgttca gatggatagt atggctaaga tggccctgga aagtgtagaa
     5881 aaaggcgaaa tggaatttgt tccagaacgc tttgtaaaga cctatcagca ctggttagaa
     5941 aacatcaagg actggtgcat ttctagacag ctgtggtggg gccatagaat tcctgcctat
     6001 tactgcttag actgtggaga aatgatggta agcagacaag aagtaacaag ctgcacaaaa
     6061 tgcgcaggca ctaagataga gcaggatgaa gacgtgctgg atacatggtt tagttcagcc
     6121 ctatggccat tttcaaccct aggctggcct gataaaaccc ctgagatgga gtacttttat
     6181 cctaccgatg tcctaattac aggctatgat attatttttt tctgggtagt aagaatgatg
     6241 ttttctggtc ttaaacatat tggcatagcg ccctttcacc ataccctaat caatggaatt
     6301 gttagagacg cccagggaag aaagatttca aagagcttag ataatgggac agatcccatc
     6361 gttattattg atgaatatgg agcggacgcc cttcgtttta tgcttataag tggaacttct
     6421 gtgggaagcg atacccgatt tttaatagaa aaagtagaag cagcaagaaa ctttgccaat
     6481 aaactttgga atgcttctcg ttttctccta actgatctag agatgaaggc ctactctata
     6541 gaagaaaagc agctagatct ttctgataaa tggatcctat ctagactatc acaaacaact
     6601 caggcagtag aaagaagtct ggagcgctat gaactaggac aggctgctga tcgagttctt
     6661 gattttgttt ggagtgatta ttgtgactgg tttattgaac taagtaagga agctctttat
     6721 agtggggatg aacaaagaaa agaaagaaaa caagcggtgc ttttatttgt tcttgaagat
     6781 atcctaaaac ttcttcatcc ctttatgccc tttatcacag aagaaatttg gcagcacctg
     6841 ccccagagga caagccatct tatctgcagt gcttggcccc tagcaggtaa gacttatcct
     6901 gaggaagaaa acctaatggc tcttttacaa gaagctatca gtcgaattag aaatattcga
     6961 gccgagatgg atattcctcc ttcaaaaaaa ggcagactct tcctacaggg ccccagggca
     7021 gaggaagttc ttacgtatgc agggtatttt agggcaatgg cctctagtga tgaaattagt
     7081 ttggcagagg agcaagtagc agacgcttct gccatcttgc tagatggact gagtctctac
     7141 cttccccttg atgaactaat tgatcatgaa aaagagctta agcgcctaga aaaagaagaa
     7201 aataagttgg attcagaaat taaacgcagt agggggaaac taaacaatgc aggttttgta
     7261 gccaaggctc ctgctgagtt ggtaaaaaaa gaagaagaaa aactagaatc cttcctacga
     7321 cttcaagagg agttagagga aaaaatcata ttagcaaagg agaaaattca gtgaaaatcc
     7381 aggaagccct gaattttttg aatcagtcct ataaactagg cagaaagcta ggacttgata
     7441 atataaaaag actcttacta gagctgggaa gtcccgaaaa aaagatgaaa gtcatccatc
     7501 ttgccggaac caatggcaaa gggtccacca gcgctatgat ctaccatgtt attttagaag
     7561 ggggatataa ggtgggtttt tttagctccc cccatctagt ccactataat gaacgctttc
     7621 agtataatgg aaaaaccatt agcgacacgg aactggcaaa gagcatagaa aaggtaaaat
     7681 ttgcctgtga gaggcttcta gcacaaggat atgatcatcc aacagaattc gaaatcctta
     7741 ccgctgtcgg ctttgtgttt tttgctgata agggtcttga ctatgcagta atagaggtgg
     7801 gtcttggagg aagacttgat gcaacaaact ctgtagagga gccccttgta tcggtaatta
     7861 ctcctattgc tatggaccat gaatcctacc tgggggacaa tcttctagcc attgcagggg
     7921 aaaaggccgg tattataaag cctcaaattc ctcttgtaag tggattgcag caaaaggaag
     7981 tattggatct tttaaaggat aaggcaagaa aaaaaggaag ccccttttac cctagcgacc
     8041 cctcccagct tgaaataatc cgcagtgatc tactagaaaa catctttcgt tataagggca
     8101 gagagtacaa gctgaaactt ctaggaagac atcaaattga caatgcgatt ttggccctag
     8161 aaaccctcta tcttttacga gaaaaaggtc aaatagacct tgaagaagag caaataagaa
     8221 ggggactctt agagactcgc tggcccggtc gtttggagaa gatttcgcaa aaaccggata
     8281 tctttattga tgggggccac aacgctcacg gtgtgcgggc tgtggctcaa aacctcttat
     8341 tagatgaaaa aaatcgtcgc cttcttcttt taggcatgcg cttagacaag gactataaag
     8401 aggtccttag tcttcttttg cccttgtttc gcgaagtcgt tcttactgag cccctgggag
     8461 accaggttgt tttggtagaa gaccttcaaa aagaagcagc ccttagggga gtaatggctg
     8521 agggtgatag ggactataaa aaagctctta gtctggtttt ggccaaggca aatgaagaag
     8581 atcagatttt tgttatggga agtttttatc tgattggcca agtaaaagag gaggtcctta
     8641 ggcgaatagg agtagaaaaa tgattgttct tatttcagga tctccacgaa gaagggagct
     8701 tctttcacgc attacttcca aggtgattta tgatgttagc catgtccagg aaccagtggg
     8761 tgaaaaagat ccgcaaaaac tagtgataaa aagcgccaga gccaagctta aggatcgcct
     8821 gcatgtccat aaaaagggaa tccttcttgc ctgtgatacc attgtctatg cggataaagt
     8881 tttaggcaag cctaaaaaca gagaggaagc ctctcaaatg ctggcttttc tctcgggccg
     8941 cagccatcaa gtctacacta gtttggttct ccatgataag aataagggca ggcttgtaga
     9001 ggaggtggtt tgcaccaagg tttatttttc ctctttagag gaaaaggaga tccaggccta
     9061 tctagataca ggagagtatg tagataaggc gggaggctat gggattcaag gctttggtag
     9121 tcttcttgtt gagaaaattg aaggagatta ctttaacgtt atgggcctac ccctaaaccg
     9181 tctctattta attttgaaaa aagagttttc tcttgatcta ttaagagata tatagtcttt
     9241 ctaaaaagga agagtcccct gagggaagct tcctttttac ttttcccctc tatactaaaa
     9301 ttggaggtgg agtatgaata taccaataag acaattgccc tcttgggaac gtccccggga
     9361 aaagatttta gaaaaaggag tagaaagcct ttccaatgcc gaagtcttgg ccgttttatt
     9421 aggaagtggc aactccaagc aatcggccct tcttttggct caagaacttt taaccagttt
     9481 ggacaagggc ttatatcagc tagaagaaat aactatttac gagcttatgg actttccagg
     9541 cataggacct gctaaagcct gcagaatatt atgtggtctg gaactggctc gcagggtcaa
     9601 ggcagcccca gcaaaaccct taaatgtctc atcgcccgga gcggtttaca gctatatgct
     9661 ctccaaaatg caaaactacc aaaaggaagt ctttgtctgt cttcatctgg atagtaaaaa
     9721 caatattatt agtgaggaca ttgttagtat cggtactctt aatcgttcct tggtacatcc
     9781 aagggaggtc tttaaaaaag cagttaaaaa tggagctgca tccatactgg tagtccacaa
     9841 tcatcccagt ggagatccaa atccctccca ggaagataag caggtaacaa gtcgattaaa
     9901 ggaagtggga gagcttctag gtattccctt actggatcat cttattgtgg caagagacag
     9961 tcgctttagt tttcgcgagg cggggatctt ttgatatcta cttatttttc ttgtcttaag
    10021 gtataataag gctagtgagg taaaaaatga aattagaaca attattaaaa caggtagaaa
    10081 aaccgactcg ttatttagga gaagagcggg gttctgttat aaaaaaccca gatgaggtaa
    10141 aggctgattt tcttttggcc tttcctgatc tttatgaggt aggaatgagc catatgggcc
    10201 ttcatatcct ctatggttta ctaaatgaaa aagaagactt cttatgcgag cgcctatttg
    10261 ctcctgctgc ggatatggaa aagttaatcc gagagggcaa gactcccttg ttttctattg
    10321 aaaacaaaag gcctatgaaa gactttgata tgattggctt tacccttcag tatgaaatga
    10381 gttttaccac cattcttgga atgctagagt tggggggact tgctcttaga tccaaggacc
    10441 gggggccgga agaccccctt attatagccg gtggcccttg tgcctttaat ccggaaccta
    10501 tgagcgcctt tattgatctt tttgtcctag gagatggaga agaagttctg cttgaactaa
    10561 tggagctctg gcttaagaca ggcaagaagg ataagtttct aaaagaagcc ctaaaaatca
    10621 gaggggtcta tgttcccaag tactatgagc ctgagtttag tgggggtctt tataagggac
    10681 tggctcctct aattgaggag gcccccctaa ccatacaaaa ggccctgata aatgaccttg
    10741 atcattgcta ctatccaaaa aagatgattg ttccctatat ggatattgtc catgataggg
    10801 cagtaatgga gatctttcgg ggatgcacta agggttgccg cttttgtcag gcgggcataa
    10861 tctatcgtcc cttaagagaa agaagtgtgc agacccttgt aaaggccatc gatagcctgc
    10921 taacagaggg gggctatgat gaggtgggat taagctcact ttccaccctt gactattcgg
    10981 ggattttgcc cctagttgaa gaattaaccc aaacctatag tgaggatcat attcgcctta
    11041 gtctgccttc cctgcgccta gacacctttt ccattgatgt gctacgactt gtgcaaaaaa
    11101 ggcgcaaggg ttctttgacc tttgccccag aagcgggaag tcagaggatg cgcgatatta
    11161 tcaataaaaa tgtaaatgaa aaagatcttt tttccaccat ggaagaaatc tttaaagaag
    11221 gttataagaa ggtgaaactc tattttatta tgggacttcc aggagaaaat atggaagatg
    11281 tagaagccat tgcagagcta gcttataaag tggtggacct ttataaagag gtgacaggca
    11341 aaagaaatgt tgagattacc attagcacct cctgtcttgt gcccaaaccc ttcaccccct
    11401 ttcagtggat ggctcaggaa aaagtggagg actttgaaga aaaacagcgc tatctaagag
    11461 ggctactgcg aggcaagagt tttcgataca attatcacga tgccaaaacc tctgtggtgg
    11521 aaggccgtat ttgtagaggc ggcagaaaaa tggcagatgt acttgagctt gcctacaaaa
    11581 aaggagcctt tttagaagct tggatggaga actttgatga aaccatctgg caagaggcct
    11641 ttaaggagat gggtatggac tttgaccagg agcttagttt ttccttagag gaaaccttac
    11701 cctgggacca tatcgatgta ggggtcaaaa aagagtttct caaaaaagaa tggatggcag
    11761 cacaaaaggg ccagctgacc ccagattgtc gccaaggttg cctgggctgt ggagtaaact
    11821 tagaaagctt tggtggggtt tgctatggta attagattat ggatgataaa agaaggaccc
    11881 atgatgtttc ttagtcacct agaacttatg agaactcttg agcgctgctt taaaagtgcc
    11941 aggctaccta tttcctattc ccagggcttt aatcctaggc cccttatgaa ctttgctttt
    12001 cccctagcag taggggtgga agcagataag cttttacttg aaattgaatt aaaagaaggg
    12061 gccgatccca gtcttcttga aggggtagac tttcccagag gcctagagct tcaggactat
    12121 aaagtggtag aaaagggacc ttctcttatg agcctggtga tctcagctag atatcatata
    12181 agaggggata agaaaaaact gcaaaaaatt aaaacagaag actccttgcc ctttttaaaa
    12241 agaaacaaaa gaggacgtta taataagaaa gaagcacgtg agtttattct ttcttataag
    12301 gaaaaagagg atggctatga ctttcttctt aaggcaggaa gcagggccaa tttaaaagcc
    12361 acagacctgc ttatggccct tttagaaaag gaagaggaag tacacgccta taatgtgaga
    12421 ctattagatg tatatgatga taagggcaaa agcctatggt aattagctat gaaacagata
    12481 gttttagacg aagatttatt tttgaaggag aaaacctaat ccagggacgt agctttctaa
    12541 aagaaggaga aagaggagaa gtctaccgag gaaaagtttt aaaaagggta ggaaacctag
    12601 gctactttgt cgatcttgga ccttttaagg gactttgtca agaaaagtta aactcaggag
    12661 atattcagct ttttgaaatt ataaaaagac aagtagataa ggagcccctt cttactagta
    12721 aggtaggatt tatgggagaa agcctaatct tgcaggatgg gccaggcatc tactactcaa
    12781 agtcaattcc ctcttcttat cgtaggctgc ttgaagagct caagcttgag ggagtttatt
    12841 ttcgcaggga ggccagtcgc cttcccttgc atcttgttaa aaaggagtat gacagcctta
    12901 gagcaaaaaa aaaggccctc cagaataaaa accagctggg ccttgtttat agtcccccct
    12961 ttaaggaaga aggcgatccc ttttacttta tggagttgga agaaaaaatt ctccatctgc
    13021 gccgtggaaa gtcgaaaaaa gacgggataa ctcttttttt tgagcccacc agggcaggac
    13081 ttgttattga tgtcaatgga gtgggaaaag aagaggatat aaataggaag gctggggaga
    13141 tgattgaaga aagtcttatt ctaatgaatg taggaggcct tgttataata gatcttctag
    13201 gaagaggtag gggctatagg ggggagggtt ctcttacaaa agagggaatt tttgttaaaa
    13261 gcctgcctgt gcgaggagca aatttatttt atgtcccctt agaaaaacta aagaatgatt
    13321 ataaaaaact gcgaaataaa cttagcgagg aatatgaatg aaagaagtta atctatttga
    13381 tattttagga ccagttatga ttggccccag tagctcccat acagctggag cagtcagact
    13441 gggaaatatc gctgccatga tttataataa aaaaattact agggtagatt tttatctcca
    13501 tggtagtttt gccaacacct ataagggcca tggcacagat cttgccttag tagccggtat
    13561 actgggtctt aaaacctatg atgaaaaaat agttgatgcc tttagtttgg ccgatgaaga
    13621 aggactggct ttttcttttt atagaaaaga tcttggatat gttcatccca atacagtaag
    13681 gattgttttt gatgaaaact ttagtgttac agcttctagt ctaggagggg gccgggtaga
    13741 gattattgat attaatggca ctgaagtatc tcttcaaggc gattatccta ctcttgttct
    13801 ccaatatgaa gataaattag gcattattca agaagtaagt gctcttatag cagcaacagg
    13861 tgttaatatt gcagcaatga aagttacaag gcaaagaaaa catgcaacca tggttcttga
    13921 gcttgatggg aaaatcaaca aagagacctt tgagtcactc aaagtcctag ggttaaacta
    13981 tttggcaagt gttggcgcag tggaggaaaa atgatttata aggccgcaga tattttaaaa
    14041 aagtgcgaag agaaaaattg tagtattgct gaactgacta ggcaagatga agctgccacg
    14101 gcttctataa gtgtagagga attagatctt aagatgaaag aaattcttga aattatggaa
    14161 acttcttctt ctcttggcct taaagaagaa atattttcgc tatccggcat gaccggggcg
    14221 agcgccaaaa aactttggga taatagagat cagacttctt taagtgggga gtttattctc
    14281 tctgccatgg caagggctct ttcgacctct gaggttaatt cttctatggg ccgtattgtg
    14341 gccgccccta ctgctggagc ttctggaatt ttaccatcag cacttatgag tgcaaaggaa
    14401 aaactatctc taggggggga agaattgatc ttggggcttt atactgctgg tgcggtggga
    14461 aaaatcgtgg ctcaatcggc taccatcagt ggcgctgatg gtggttgtca agccgaatgt
    14521 ggcacagggg ctgctatggc cgctgctgct ttggttgagc tgatgggggg ctcgccccag
    14581 ctagcctttc atggagcaag ctttgctttg attcatatta tggggcttgt ctgcgatcca
    14641 attgctggat ttgttgaatt cccctgttct ttacgtaatg cctcaggcat cgtaaatgcc
    14701 tttataagcg ctgatcttgc tatggcaggc cttagttcac ccattccctt tgatgaggtt
    14761 gtagacgcca tgtatcaggt aggaaagatg ttacctgagg cacttcgcga gactgctcta
    14821 ggtggcatag ctgcaacgcc ttctgccaag gcattagaag aaaaatattt aggcaagggc
    14881 taaaaatata ataaaagtta ttt
//
LOCUS       DASRNA010000177         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_650420,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000177 DASRNA010000000
VERSION     DASRNA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650420"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1850
                     /locus_tag="VFD08_04960"
     CDS             279..1850
                     /locus_tag="VFD08_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HZK10401.1"
                     /translation="MSRFLKKYIIVIAVLFCFALFPSSSYAAGEIFSVTVPMNLPITM
                     DSSGQVTTAQNAAITNNSSAAVKVTKVKAKLNGWIINPNLSPSTTKVDSKEIALWIQG
                     HSVLPDGNLQGGVSNWEPIRKNFPMSISYDARLPAQLDDCNELIGHIVYTFVSLERVT
                     DDGNYILNIETGTIVKYIGTNKNDLVVPSTLSVDGQVYQVKTIGENAFQNQELKGSLT
                     ISQGIKTIGARAFYGNDLKYVGIPNSISTIGSCAFCYNRIGSFPIWEGRVTIDNYNGG
                     IPNLESNAFNNNYFFQRYPAPVEYLRSAPSRSSLNSSSTESYHVKELYFIDFDENGQP
                     VFDEEGYLVCDVDEIERLGYLEIFIEKGLLPAFQEIEVKEKVLPILEVDPLKEGDTSI
                     TGKTNPGATIQIENSSQELKTQADNKGDFIFDLSPLKADEIISIRVVEDDDLLELVKE
                     EKVKALDLEKEEEVPVERDLTSDEDPVVRDEKPQGSVDSTLKAPEDAKTEDARDVQTN
                     KIITPPAILEEEEEL"
     gene            1971..2552
                     /locus_tag="VFD08_04965"
     CDS             1971..2552
                     /locus_tag="VFD08_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10402.1"
                     /translation="MKKRISLLLLFIMILALNTTALASNVSVNPADFTNLEQPGRSTS
                     TVVLTADAVQFSVIVPTKLPLHIGSDGGVTTATNAKFINRSYAPVRVSQAEIKATEGW
                     TLAPYERNIKEMDLASQEVGFKIHGVATLSKGSKEVLLFDKDKIQIQGRDPGASENTT
                     FSLSYKATVPVQTKSIANEKKIADVVFTIGWDM"
     gene            3116..3544
                     /gene="rplM"
                     /locus_tag="VFD08_04970"
     CDS             3116..3544
                     /gene="rplM"
                     /locus_tag="VFD08_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZK10403.1"
                     /translation="MKTFVAKPAEVEHKWYVVDAEGKTLGRLASEIAKILRGKHKPIY
                     TPHVDTGDYVIVVNAQKVHVTGNKRQDKMYRWHTGYMGGLKERNFQEMITRQPEKVIM
                     HAVKGMLPKNSLGRQMAKKLRVFAGPEHTHSAQQPEVLDL"
     gene            3553..3942
                     /gene="rpsI"
                     /locus_tag="VFD08_04975"
     CDS             3553..3942
                     /gene="rpsI"
                     /locus_tag="VFD08_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZK10404.1"
                     /translation="MAIQFQGTGRRKKSVARVRLVPGTGEIVVNKRPVAEYFDHEIQI
                     QEVKRPLEITGAAGSFDVFVNVHGGGFTGQTGAIRHGISRALVNADEANKPKLKSAGY
                     LTRDPRMKERKKYGLRKARKSPQFSKR"
BASE COUNT         1516 a          708 c          913 g         1262 t
ORIGIN      
        1 ctctagtgat tatcttggtc cagcttctag aataattcct ggtattggtg atttagggga
       61 aaaatcagat ttttctatta attatagagc aaaggttcct gcaagagaaa cggcaatagc
      121 tgacactgtt gtagctaatg tagtcttcac ctttgcttgg gatacaacaa cggattaatt
      181 tagcgtttta attaggtaaa gacaggtaat tgcaccacct gtcttttgtc ctaataaata
      241 aaaaagatag tacataataa aagaagggag ttgataggat gagtagattt ttaaagaagt
      301 atattatagt gattgcagtt ttattttgct ttgcattatt tccttctagt tcttatgctg
      361 cgggcgaaat ctttagtgtt actgtaccca tgaatctacc cattactatg gactctagcg
      421 gccaggtaac aacagcacaa aatgctgcca tcacaaataa cagttcagct gcggtaaaag
      481 ttacaaaggt aaaagcaaaa ttaaatggct ggattattaa tccgaattta agccccagta
      541 ctacaaaagt ggattctaaa gaaattgccc tgtggataca aggtcattca gttttaccag
      601 atggtaactt gcaaggagga gtttctaact gggagcccat aaggaaaaat tttcctatgt
      661 ccatttctta tgatgcccgt ttacctgctc agttggatga ttgcaatgag ctgattggcc
      721 atattgttta tacttttgta agtttagaaa gagtaacaga tgatggcaat tatatcttaa
      781 atatagagac cggcactatt gtaaagtata taggaactaa taaaaacgat ctagtagtac
      841 catcaactct atcagtagat ggtcaagtat accaggtgaa gactattgga gaaaacgctt
      901 ttcaaaatca agagcttaaa ggaagtctta ctatttctca aggcataaaa acaattggag
      961 ccagggcatt ttacggtaat gatttaaagt atgtaggtat tcctaatagt atatcaacca
     1021 taggcagctg tgctttttgt tacaatagaa taggttcatt ccctatatgg gaaggcagag
     1081 ttactattga taattataat ggaggaattc caaatttaga aagcaatgcc tttaataaca
     1141 attatttttt ccagagatat cctgctcctg ttgagtattt aagaagtgct cctagcagat
     1201 catccctaaa cagttcttca actgagtcct accatgtaaa agagctttac tttattgact
     1261 ttgatgaaaa tggtcaacca gttttcgatg aagaaggata tctggtctgt gatgtggatg
     1321 aaatagaaag attaggctat ctagagatct ttatagaaaa aggactcctt ccagctttcc
     1381 aggaaataga ggtcaaagaa aaagtccttc ctattttaga ggtagacccc cttaaagagg
     1441 gcgatacttc tatcacagga aagacaaatc caggagctac tatccagata gaaaactcat
     1501 cccaggagtt aaagacacaa gcagataata agggtgattt tatatttgac ctatctccct
     1561 taaaagcaga tgaaattatt agcataagag tagtagaaga tgatgatctt cttgagttag
     1621 tcaaagaaga aaaggtaaaa gccttagatc ttgaaaaaga agaagaggtg ccagtagaaa
     1681 gggatctaac ttcagatgaa gaccctgtag taagggatga aaaaccgcaa ggctctgtag
     1741 attcaacttt aaaggctcca gaagatgcta aaacagaaga tgctagagat gtgcaaacaa
     1801 ataaaattat aacaccacct gctattctag aagaagaaga ggagctttag ttttagttaa
     1861 aataagtgga ggtgatatct caagttttta cggttgattt atcacctctt taatattaat
     1921 agatagaaaa atcaaatata gtccaaaatc taaagatagg agtcaatcat atgaaaaaac
     1981 gtatctctct actgctttta tttatcatga ttttagcgct aaatactaca gctctagcat
     2041 caaatgtatc agtgaatccg gcagatttta ctaatttaga acaacctggc cggtccacat
     2101 ctaccgttgt attgacggct gatgctgtac aatttagtgt aatagttcct acaaagttgc
     2161 ccctacatat aggttctgat ggaggggtta ctacggctac aaacgctaag tttattaata
     2221 ggagttatgc tcctgttcga gtaagtcaag cagagattaa agcaacagag ggctggactc
     2281 ttgctcccta tgaaagaaat ataaaggaga tggacttagc cagccaagaa gttggcttta
     2341 aaatacacgg tgtggctact ctatcaaagg gcagtaaaga agtcttgctt tttgataagg
     2401 acaagataca aatccagggg agagatccgg gagcatcaga aaatacaact ttttccctta
     2461 gctataaggc cacagtgcca gtacaaacaa aatcaatcgc aaatgaaaaa aagatagctg
     2521 atgtggtatt taccattggt tgggatatgt agcagtgcct gatgctttag atataataat
     2581 ctaaactgaa gaagacgctt aagtcagaaa acctatctat ttgactaggg tggctatagg
     2641 acaagattta ctagtcctcg tagtaaatct tatctaataa gtggggcctg ttttatttct
     2701 ggattaaaaa tcaggatatt ttaccagacc tagatagagc agaagccttg tctaagatgg
     2761 gacaaggctt ctgctgctag tgggatggtt cttattgcaa gtaagaagaa aattctagaa
     2821 aagaaggggc tgacttttta taaatctatt ttttaaaggg taggtgctta ggacctttta
     2881 aaggggcaag aaagtcacct ttggtagata taatctctta tgctaaaaga gggaaaattg
     2941 tttcctaagc taagttggac acctaaaaaa gttagtgccc agtttacttt ggaaagttag
     3001 ctaatagggc aaatcattga aaaaacgaag attctgcttg acagagctgg caagctctta
     3061 tagaataatc atggtaatcg attttccctg cccacaaaat agggaggata aaaaaatgaa
     3121 aacatttgtt gcaaagcctg cagaagtcga acataaatgg tatgttgtag acgctgaagg
     3181 caaaacttta ggacgtttgg cgtctgaaat cgccaaaata cttcgaggca agcataagcc
     3241 aatttacaca cctcatgttg ataccggtga ttacgtcatc gttgttaatg ctcaaaaggt
     3301 ccatgttact ggaaataagc gtcaagacaa gatgtataga tggcatacag gttatatggg
     3361 tggtttaaaa gaacgtaact tccaggaaat gatcactcgt caacccgaaa aagtcattat
     3421 gcatgctgtt aaaggaatgc ttcctaagaa ttctttagga agacagatgg ctaaaaaact
     3481 acgcgttttt gctggtccag agcataccca tagtgcacaa caacctgaag tactggatct
     3541 ataggaggaa atatggctat tcaatttcaa ggaactggca gaagaaaaaa atctgtcgcc
     3601 cgtgttcgtc ttgttccggg tacaggagag atcgttgtta acaaacgacc tgtagcagaa
     3661 tattttgatc acgaaattca aattcaagag gttaaaagac ctttagaaat tacaggagct
     3721 gcaggcagct ttgatgtatt tgtcaatgtt catggtggag gatttactgg tcaaaccggt
     3781 gcaatccgtc atggaatcag ccgagcgctt gttaatgcag atgaagcaaa taaaccaaaa
     3841 ctaaaaagtg ctggttattt aacgcgagat cctcgcatga aagaacgaaa aaaatacggt
     3901 cttcgtaaag cgagaaaatc tccacaattc tcaaagcgtt aagtttactt cttgtccaaa
     3961 agagccctta tggggctttt ttttatcggt ataagctgtt attgcaagtg gatcataaga
     4021 ctagtatgtt ttttgaaaaa aaccagacag ggaaagaatc ctttgtgaag taagttttgg
     4081 cagaaaaaga gtagaaactg ggctaggctt ctagcttggc ttgttaaaat aaaagataaa
     4141 agtattgatt aagacaaagg ggtcttatta taagtataaa ttgacttaaa agaaacaatt
     4201 tatagacaat taggttgaac gaaattaaca ttcattcaga aaagaagcgg tagaatataa
     4261 ttctgtatat attatgccca gcccagccca atgatataat ttaaatttga caactttctc
     4321 ctataggggt agaataaaag tatccattac atgggatagg gtaaatcttt ttctaaagga
     4381 cctttaggta agaaatttt
//
LOCUS       DASRNA010000178         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_574376,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000178 DASRNA010000000
VERSION     DASRNA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574376"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VFD08_04980"
     CDS             <1..1213
                     /locus_tag="VFD08_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10405.1"
                     /translation="YFIDYENAWDFTSNLDDFLHEYIQIMIDKKQLKEAFELLAYLFL
                     KLARQGVDDSSGGTDLVADRCQEMWKEIIDKSELSLKKEQFKWFIEHVDSPDTDYMGE
                     YLEEVLLEKFKEEEFHDHKLLYIEKKSQGYRKEELSRYRRRKLGELALRHIEILELKK
                     ISGKEIESYCKDNLELSLIREYLVDFYLEKKKYDRAIKLLKEGKKRDADDREVVANYS
                     LKLKDLYKKLGEQDSYRDELWLLVLEYLPGEEELFDELKALYTKKEWIKKRERIFESL
                     SLSSDRARFYAKEGLYERLLACVVKANGLGLLRSYEKDLKDIYPQELLQKYEDTLEDM
                     ASYTTNRKYYQEIVLILKRMEAYPGGETRVNKLVNEWRQKYKNRPAMMDELKKLPFIR
                     LDLIDRFPGWN"
     gene            1399..2337
                     /locus_tag="VFD08_04985"
     CDS             1399..2337
                     /locus_tag="VFD08_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK10406.1"
                     /translation="MENSKGNIFQNPYLVTALALFSCFLWGSAFPTIKVGYELFAISG
                     SNTYGKILFAGYRFFLAAFLILTFCLLTGREIKIRKGQIKGLLLLGLLQTAINYTFFY
                     IGVSNISGTKGSILSATGTLFSVLLAHFFYKEDKLTKKKIIGLIVGFSGVVLVNINGG
                     DVGGGFSLTGEGFVLMASLTGAISGIYTKALARHINTFLITAYQLLIGSLFLISVGFL
                     GGAQSLTFTGQNIWLLLYLSFLSAAAFSIWTILLKHNGVGKVSIYKFTVPIFGTFLSY
                     IILKERLLGTNTILAIILVSLSIILINIDRVKKSST"
BASE COUNT          819 a          354 c          512 g          700 t
ORIGIN      
        1 ctattttatt gattatgaga atgcttggga ttttaccagc aatctggatg atttcctgca
       61 tgaatatata caaataatga tagacaagaa gcagctaaaa gaagcctttg aattgcttgc
      121 ttatcttttt cttaaactag caagacaggg tgttgatgat tcaagcgggg gaacggacct
      181 agtggcagat cgctgccaag aaatgtggaa ggaaataata gataagagcg aactttcatt
      241 aaaaaaagag caattcaaat ggtttattga acatgtcgat agtccagata cagattatat
      301 gggggaatat ttggaagaag ttttacttga aaagtttaag gaagaagaat tccatgatca
      361 caagctccta tatatcgaaa aaaaatctca aggatataga aaagaagagc tatcgcgcta
      421 cagaaggcga aaacttggag agctggccct gcgccatata gaaatcctag agttaaaaaa
      481 gataagcggg aaggaaattg aaagctactg caaagacaat ttagaacttt ctcttattag
      541 ggagtacctt gtggactttt atcttgaaaa gaaaaaatat gatagagcca ttaaactcct
      601 taaggaaggt aagaagaggg atgcagatga tagggaggtt gtggcaaatt atagcttgaa
      661 gctaaaggat ttatacaaaa aactgggaga gcaggattct tatagggatg agctatggct
      721 cctagtgctg gaatacctgc ctggagagga agagcttttt gatgaactaa aggccctcta
      781 tacaaagaaa gagtggatta aaaaaagaga aagaatattt gaaagcctgt ctttatctag
      841 cgatagggct aggttttatg caaaagaagg cttgtatgag cggctccttg cttgtgtggt
      901 taaggctaat ggtctgggac tcttaagaag ctatgagaaa gacttaaaag atatctatcc
      961 ccaggagctt ctgcaaaagt atgaagatac cctagaggat atggcaagct ataccaccaa
     1021 ccggaaatac taccaggaaa tagtgcttat tttaaagaga atggaagcct atcctggggg
     1081 agaaacaaga gtcaataaac ttgtaaatga gtggagacaa aaatataaaa acagaccagc
     1141 catgatggat gagctaaaga aactaccctt tataagattg gatttaatag ataggttccc
     1201 aggttggaac taatggtaaa gagcccttag tcaatgtatg caaatcgact aaagacaagc
     1261 cttttaactt taaagaaagg aaaaaaggct tgttttcatt tgttttttta ttatctaagt
     1321 caagatatgt ataaaagaca aaaggtggta taatacagta gatttgacaa atactaagac
     1381 aagaaaagag gaagtaattt ggaaaacagt aaggggaata tctttcaaaa tccctatcta
     1441 gtaacagcgc ttgctttatt tagttgtttc ttatggggaa gtgcctttcc taccataaaa
     1501 gtgggatatg aattatttgc aattagtgga agcaatacct atggaaagat tttgtttgca
     1561 gggtataggt tctttctagc ggcatttttg attttaactt tttgtctttt aacaggaaga
     1621 gaaataaaaa taagaaaagg ccagattaag ggccttctat tattaggact tttacaaaca
     1681 gcaataaatt ataccttttt ctatatcggc gtttccaata tcagtgggac caaagggtct
     1741 attttatctg ccacaggcac cttattttct gtgctcttag cccatttttt ctataaggaa
     1801 gacaaattaa ccaagaaaaa gatcattggc cttatagtgg gtttttctgg agttgttctt
     1861 gtaaatataa atggtggcga tgtgggaggt ggtttttcct taacgggaga aggctttgta
     1921 ttaatggcaa gtttaaccgg ggcaatatca ggtatatata ctaaggccct ggctaggcat
     1981 ataaatacat ttttaattac tgcctatcaa ctattaatcg gttcactttt tcttattagc
     2041 gtaggatttt tagggggagc gcaaagcctt acctttaccg gtcaaaatat atggttgtta
     2101 ttgtatttat cctttctctc ggcggcagcc ttttctatat ggactatttt gcttaagcac
     2161 aatggggtgg gtaaggtatc tatatacaaa tttaccgtcc caattttcgg cacctttctt
     2221 tcctatatta tcctaaaaga aagactatta ggaacaaata ccattctagc cattatactg
     2281 gtgtctctta gtattatttt gataaatatt gacagggtaa aaaaaagcag cacttaagta
     2341 tagacgaaaa tagcgatcac aggcataaag agatcaatct acaat
//
LOCUS       DASRNA010000179         4075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1102458,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000179 DASRNA010000000
VERSION     DASRNA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102458"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1164
                     /gene="ald"
                     /locus_tag="VFD08_04990"
     CDS             46..1164
                     /gene="ald"
                     /locus_tag="VFD08_04990"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZK10407.1"
                     /translation="MRIGVPKEIKDNEQRVALTPAGAAELIKHGHEVFIETLAGTGSD
                     FADEAYKAQGAKILPTAAEVWNNADMIIKVKEPLEEEYQYFREGLILFTYLHLAAEEK
                     LTQQLIDSKVVSIAYETVELPNRSLPLLTPMSEVAGRMALQEGCTFLEKIHGGKGLLL
                     DGVPGVAPAHVVIVGAGVAGAAALKRAVGGGARVTVLDVAIDRLKYLGEIYGSQIETV
                     YSNNFNLMEAMKTADLVISTVLIPGEKAPKLVTEEMVKAMPDGGVIVDVAIDQGGTTE
                     TISTPTSHTKPVVVKHGVLHYAVPNIPGAVPKTSTLALTNVTLRYAVNIANKGWKKAV
                     EDDAALAKGVNVVNGKVTYPGVARAFDLPYVELSEAIK"
     gene            complement(1203..1481)
                     /locus_tag="VFD08_04995"
     CDS             complement(1203..1481)
                     /locus_tag="VFD08_04995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK10408.1"
                     /translation="MQEIKKGKNCFYIGEKEKSPLGVIEYRPEGEVYLVTSTRVKPEL
                     RGHGVAAKLLDRLADHAREEGVKLLAQCSYVVDKFANDRRYDDIKAEK"
     gene            complement(1531..2136)
                     /locus_tag="VFD08_05000"
     CDS             complement(1531..2136)
                     /locus_tag="VFD08_05000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK10409.1"
                     /translation="MVDYHYDKRLGIDTLGFREWEDEPDYYRTESTPYIALLELARFF
                     PLEKERKVIDFGCGHGRVSFFFHDLFGLDVTGIELQEETYQELKENEVNYLQKKEIAS
                     SPFNFICTHVEDYKLRDEDVFYFFNPFSQKVFKKVLANIEKSLEVRDRECFIILYYPF
                     VDYMYYLEKYSSFERVLRVPLSWEKDEREVFLIYRHLPWRD"
     gene            complement(2153..2803)
                     /locus_tag="VFD08_05005"
     CDS             complement(2153..2803)
                     /locus_tag="VFD08_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10410.1"
                     /translation="MNYQKKIHTWGRVSLLVALGLFISFPLISSIYFDAWPGWMPVFE
                     GLLAIAPIYWTVGVIEALTFSPMLGNGGSYLGFVTGNLTAMKVPAALQAMEQAGVEPG
                     TPQGEVVSTIAIATSSVVTTLMLFLGMLLLSVFRPFLEAPILEPAFGNILPALFGGLG
                     VVFIAKNWKLAVAPLLMMSLLFIIRPSLASAVSVLVPVSALITIGVGRILYKKNLL"
     gene            complement(2812..3531)
                     /locus_tag="VFD08_05010"
     CDS             complement(2812..3531)
                     /locus_tag="VFD08_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002673401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HZK10411.1"
                     /translation="MFSINHPLLFALAAIVIVFVLGQSFFFFYQAWKEALRLGLKKKM
                     LKRLVLSTAVFTLAPALAILLGLLSLSKFLGLPLPWLRLSVLGALTYELPAAASVANI
                     MNLSLDQPLQNAVAFNAMAWVMTLGILSGILVILFFQKSMASGLRKVRAKDEAWSKVL
                     MDSLFIGMISAFLGMVFSDVGSGLAGMIPVFVLVFSALLMIFWGFLIKRYKWKWLESY
                     ALPLSMLGAMAFAIPLTYWLI"
     gene            complement(3524..>4075)
                     /locus_tag="VFD08_05015"
     CDS             complement(3524..>4075)
                     /locus_tag="VFD08_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HZK10412.1"
                     /translation="ESWVLSNPRKYFFILEKILLKNRQTAAMVRTTVAFTMTDAGEAH
                     NVLPRRASCVANVRILPGETFEEIMDWFYSFDHEFTVDILIKEEGTKDSNIKSRFYSQ
                     LEGCIQKNFPDAIMTPYLVTGGTDSRHYKDLVENIYRFLPCRVTDDELSRMHGRGEYL
                     SIENLEKMISFYLDLLRQEGADV"
BASE COUNT         1284 a          904 c          768 g         1119 t
ORIGIN      
        1 ctagtagtga tataataaag ttaatactat tttatggagg tttttatgag aatcggtgta
       61 cctaaggaaa ttaaagataa tgaacaacgc gtcgctctaa ccccagcagg tgccgcagag
      121 ttaatcaagc acggacatga agtttttatt gaaactcttg caggaacagg ctcagatttt
      181 gctgacgaag cctataaagc ccaaggagct aaaatcctcc caactgcggc tgaagtttgg
      241 aataatgctg atatgatcat taaagtgaag gaaccattag aagaagaata tcaatatttt
      301 cgagaaggtt tgatcctttt tacctatcta caccttgctg cagaagaaaa gcttactcag
      361 caactaattg actcaaaagt cgtctccatt gcttatgaaa ccgtagaact ccccaataga
      421 agcctacctc tacttacacc catgagtgaa gttgcaggac gtatggcttt acaagaaggt
      481 tgcaccttcc ttgaaaagat tcatggtggc aagggccttc tacttgatgg tgtacctggt
      541 gttgctcctg ctcatgtagt aatcgttgga gcgggtgttg ctggtgctgc tgctttaaaa
      601 cgcgccgtag gtggtggggc acgagttacg gttcttgatg ttgcaatcga tcgcctcaaa
      661 tatcttggtg aaatttatgg tagccaaatt gaaacggtct actccaataa ctttaatctt
      721 atggaggcaa tgaagactgc tgatcttgtc attagcactg tcctaattcc tggagaaaaa
      781 gcgccaaaac tagttacaga agaaatggta aaagctatgc ctgatggcgg agtaattgta
      841 gatgttgcta ttgaccaagg tggtaccact gaaacaatat caactcctac atctcataca
      901 aagcctgtgg ttgtaaagca cggcgttctt cactacgctg ttcctaatat tcctggagct
      961 gtccctaaga cctcaaccct agccctaacc aatgtaaccc tacgttatgc tgtaaatatc
     1021 gctaataagg gttggaaaaa ggccgtagaa gatgacgcag ccctagctaa aggggtaaat
     1081 gttgtaaatg gtaaggtcac ctatccaggt gttgctagag cctttgatct gccctatgta
     1141 gaactatcag aagcaattaa ataactagat ctagaataaa tagagaggga atcattccct
     1201 ctctattttt ctgccttaat gtcatcatag cgtctatcat tagcaaattt atcaacaaca
     1261 taggaacatt gagctaagag tttgacgcct tcttcccggg catggtccgc caacctatca
     1321 agaagtttag ctgctacccc atgtcctctt aactctggct ttaccctggt agaggttaca
     1381 agataaacct caccttctgg cctatactct attacgccta ggggcgactt ttccttttca
     1441 ccaatataaa aacagttttt tccttttttt atttcctgca tttttcctcc tttaaccagt
     1501 ctcttctcgc tttataccca aagtaagatt ttaatctctc catggcagat gacggtatat
     1561 taaaaagact tcccgctcat ctttttccca ggataggggt acccgaagca ctctctcaaa
     1621 ggagctatac ttttcaagat agtacatata gtccacaaag ggatagtata aaataataaa
     1681 acactcccta tctcttactt ctaaggactt ttcaatattt gctaacacct ttttaaagac
     1741 tttttgcgag aaaggattaa aaaagtaaaa cacatcttca tcccttagtt tgtaatcctc
     1801 tacatgagtg cagataaaat taaaaggaga ggatgcgatt tctttttttt gaagataatt
     1861 cacttcattt tcttttagct cttggtaggt ttcttcttga agttctatgc cagtaacatc
     1921 aagaccgaaa agatcatgaa agaaaaagct tacccttcca tgcccacagc caaaatctat
     1981 tactttcctt tctttttcca agggaaaaaa acgagccaat tccagcaacg ctatataggg
     2041 tgtggactcc gtgcgataat aatccggttc atcctcccat tccctaaaac ctaaggtgtc
     2101 aatacccaat cttttatcat agtggtaatc aaccatttta ccctcccgag tcttaaagta
     2161 gatttttctt gtacaggatt ctgcctacac ctattgtaat aagagcactt accggcacaa
     2221 gaacacttac agcagaagct agagagggcc ttattataaa taagaggctc atcataagaa
     2281 gaggtgccac agcaagcttc cagttttttg caataaatac cacaccaagc ccaccaaaaa
     2341 gtgctggtaa aatatttcca aaagcaggtt ctagaatcgg agcttccaaa aagggacgaa
     2401 ataccgaaag taagagcatt cctaaaaaaa gcataagagt agttacaact gaactggtgg
     2461 ctatagcaat cgttgatact acttcaccct ggggggttcc aggttcgaca ccagcctgtt
     2521 ccatggcctg tagggctgct ggaaccttca ttgctgttag atttcccgtc acaaagccca
     2581 gatagcttcc cccatttccc agcataggac taaaggtcaa agcttcaatc actcccaccg
     2641 tccaataaat aggtgctatg gctaataaac cctcaaatac tggcatccag ccaggccaag
     2701 catcaaagta tattgagcta ataaggggaa aggaaataaa aagacccagg gccacaagca
     2761 aggacactcg gccccaagta tgtattttct tttgataatt cataagtcct cctaaatcaa
     2821 ccaataagta agtggaatcg caaaggccat agctcccagc atacttaggg gtagagcata
     2881 gctttcaagc catttccact tatacctttt aataagaaat ccccaaaaga tcattaataa
     2941 agcggaaaac actagcacaa aaacaggaat cattcccgct aggccacttc ccacatcaga
     3001 aaaaaccatg cctaaaaagg cagaaatcat tccgataaaa agagagtcca tcaaaacctt
     3061 actccaggcc tcatctttgg ctcggacctt tcgaaggcca ctggccatgg atttttgaaa
     3121 aaacaaaatc accagaatgc ccgagagaat tcccagagtc ataacccagg ccatggcatt
     3181 aaaggccact gcattttgta ggggctgatc taaggataaa ttcattatat ttgccacaga
     3241 agctgccgct ggaagttcat aagtaagagc ccccaaaaca gaaagtctta gccatggcag
     3301 gggtaggcct aaaaatttcg ataaactcag caggccaaga agaatagcta gagcaggagc
     3361 aagggtaaag actgctgtac taaggaccag tctttttaac atttttttct ttaggcctag
     3421 gcgaagggct tctttccagg cttggtagaa aaagaaaaaa gattgcccca aaacaaaaac
     3481 aataacaatc gccgcaagag caaataaaag aggatggtta atactaaaca tctgcgccct
     3541 cctgccttaa gagatccaaa taaaagctga tcattttttc aagattttca atagacagat
     3601 attctccccg gccatgcatt cttgaaagtt catcatctgt taccctacag ggcaaaaacc
     3661 ggtagatatt ctcaacaaga tctttatagt gacgactgtc ggtgcctccg gtaacaagat
     3721 agggagtcat tatagcatcg gggaaattct tttgaatgca tccttccagc tggctataaa
     3781 atctactctt tatatttgag tcctttgttc cttcttcttt aattagaata tcaactgtaa
     3841 attcatggtc aaaggaataa aaccagtcca taatttcttc aaaagtctct cctggtagaa
     3901 ttctcacatt ggcaacacaa gaggcccttc taggaagaac attatgagcc tctcccgcat
     3961 cagtcatcgt aaaagccacc gttgttctta ccatggcagc tgtttgcctg ttctttaaaa
     4021 gaattttctc tagaataaaa aagtattttc taggatttga aagtacccat gattc
//
LOCUS       DASRNA010000180         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_724457,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000180 DASRNA010000000
VERSION     DASRNA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724457"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="VFD08_05020"
     CDS             complement(<1..1386)
                     /locus_tag="VFD08_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10413.1"
                     /translation="MKSYKQITYRYLKEQRNRTLLTILGIILSLAMISAIGSIMVAAK
                     DRAIQDTLENAGSYHGQFTDVSQSQIQQLTNHVDVDEVGVRELKNYATVSQVTQEERD
                     NRNVVYPYRYLEIKAYDEKALSFLPLHLKEGRFPENDQEIALEDWVLEYFDEDYALGD
                     TIKLNLGDRDTSYGEDADGYTIMTEETFIQEGEGSYTLVGITQPKRRWSGAFISDAIV
                     GSRLSMQENRNYNAFFTLSDIKNAPAKIARIAKDIGVSKDNTFYNEGLLRLYGQSIHE
                     SFNSSIMALVLFVIGLIMVSTIAVIYNSFHISALERTSQFGILRSVGATPKQIRGIVL
                     KEAGTLSLIGIPIGLFSGILAMKVVFYVISLLQGDLDVLTNMTITISPTVLVLGAGVG
                     GITVFLSALGPAIKAGRISPLEAVRNTGDLKLEPFKKVKTAKLTQKIFGIEGSIARKN
                     LGRNKKRFFITV"
     gene            complement(1383..2063)
                     /locus_tag="VFD08_05025"
     CDS             complement(1383..2063)
                     /locus_tag="VFD08_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10414.1"
                     /translation="MEVVRVENLSKSYGKGNTRVDALKDINLSIQKGEFVAVVGASGS
                     GKSTLLHLIGGVDQPSSGKVFVDGVDIYGLKESELAVFRRRKVGFVFQFYNLIPVLTA
                     EENIKLPILLDRKDLDEKHFDELMNILALKDRLKHLPNQLSGGQQQRVSIGRALAYNP
                     SIVLADEPTGNLDSKNSKEIIDLLRISVKKYNQTLILITHDLEIASQADRIITIEDGA
                     IVQNEVVK"
     gene            complement(2153..>2518)
                     /locus_tag="VFD08_05030"
     CDS             complement(2153..>2518)
                     /locus_tag="VFD08_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZK10415.1"
                     /translation="EIKGDMDSSLYGDKNWIAEALINIIKNAAEHGGEDITITLEESP
                     IFTTIVIQDNGEGIDPQHLPHIFERFYKTTSKVKPDSIGIGLNLAKLIVEAQKGSISV
                     QSKKDIGTSFTMTFLKGII"
BASE COUNT          708 a          531 c          440 g          839 t
ORIGIN      
        1 gactgtgata aaaaatctct tcttatttct tccaagattc ttacgggcaa tacttccttc
       61 tattccaaag atcttttgtg tcaattttgc ggtctttact tttttgaaag gttccaattt
      121 aagatcgcct gtatttcgaa ccgcttccag tggggaaatt ctgccagcct ttattgcagg
      181 cccgagggca gataagaaaa cagtgatccc tcctactcca gcacctagca caagaacggt
      241 aggagaaata gtgatggtca tattagtcaa aacatcaagg tctccttgta gtaaagaaat
      301 cacataaaag accactttca tagccaatat accggaaaaa agtccaatag gaatacctat
      361 aaggcttaaa gtaccagctt cctttaggac aatacctctt atttgcttgg gggttgctcc
      421 tacagaacgg aggattccaa attgggaagt tctctctagt gctgagatat gaaaactatt
      481 ataaataact gcaatggtgg agaccatgat caatccgata acaaagagga ccaatgccat
      541 gatagaacta ttaaaagact catgaatgct ttgcccgtaa agacgaagca gtccctcgtt
      601 gtagaaagta ttgtccttgg agactccaat gtcttttgca attcttgcaa tttttgctgg
      661 tgcatttttt atatcactta aagtaaaaaa ggcattatag tttctgtttt cctgcataga
      721 aagccttgat cccacaatgg catccgagat aaatgcacct gaccaccttc ttttgggttg
      781 ggtaatgcct acaagggtat aagacccttc tccttcctgt ataaatgtct cctccgtcat
      841 aatggtatat ccatcagcgt cttcaccata gctggtatct ctatccccaa gatttagttt
      901 aatagtatcc cctagtgcat agtcctcatc aaagtattcc aacacccaat cttctagggc
      961 aatttcttga tcattctcag gaaatcttcc ctcttttaag tgaaggggaa ggaagcttaa
     1021 ggctttttca tcataggcct ttatttctaa gtatctatag gggtaaacaa cattacggtt
     1081 atccctttcc tcttgggtca cttgactgac ggtggcatag tttttaagct ctcgcacacc
     1141 cacctcgtcc acgtctacat gattggttaa ttgctggatt tgactttgac ttacatcggt
     1201 aaattgcccg tgataagatc cagcgttttc taaagtatct tggatggctc tatctttagc
     1261 tgccaccatt atgcttccta tggcagagat catagccagc gaaagaataa tacctagaat
     1321 ggtcaatagc gtcctatttc tttgctcctt taaatagcga taggtgattt gcttatagct
     1381 tttcatttca ccacctcatt ttgcacaata gcaccatctt caatggtgat gatccgatct
     1441 gcttgtgaag cgatttctaa atcatgggta attaaaatta aggtttgatt gtatttttta
     1501 acagagattc ttagcaagtc tataatttct ttgctatttt tactatctaa attcccggta
     1561 ggttcatctg ccaatacaat agaggggttg taggcaaggg cgcgccctat ggaaactctt
     1621 tgttgttgcc cgcctgataa ttggttggga agatgtttta gccgatcctt taaggctaga
     1681 atattcatta attcatcgaa atgtttttca tccaaatctt tgcgatctaa aagtatgggt
     1741 aattttatat tttcctctgc tgtaagaaca ggaattaagt tataaaactg aaagacgaaa
     1801 cctacttttc ttcttctaaa tacagcgagt tcgctttctt ttaagccata gatatccaca
     1861 ccatctacaa acactttccc agagctgggt tgatccacac cccctataag atggagcaag
     1921 gtggatttac cagaaccact ggcacctacc accgctacaa actctccttt ttgtatagag
     1981 agattaatat cttttagggc atccaccctt gtatttcctt ttccataaga tttagaaaga
     2041 ttttctactc ttaccacttc catatttttt gcctcctatc atacattata cagactagta
     2101 taaagggctt atcttacaat taggtgaata tctatcttac aattttgtaa gactaaataa
     2161 ttccttttaa aaaggtcatg gtaaagcttg taccgatatc ttttttgctt tgcacagaga
     2221 tacttccttt ttgggcttct acaatcaact tagcaagatt taaaccaatt ccaatactat
     2281 ctggcttcac cttgctggta gttttgtaaa atctttcaaa aatatggggt aagtgctggg
     2341 gatcaatacc ctcgccatta tcttgaatca ctatggtggt gaatatagga ctttcttcta
     2401 aggtaatggt gatgtcttct cccccatgtt ccgctgcatt ttttataata ttgattaagg
     2461 cttctgcaat ccaattttta tctccataaa ggctgctatc catatcacct ttgatttc
//
LOCUS       DASRNA010000181         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1103241,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000181 DASRNA010000000
VERSION     DASRNA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103241"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VFD08_05035"
     CDS             <1..1122
                     /locus_tag="VFD08_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HZK10416.1"
                     /translation="VGIVQNKVGIKDQGIELKEELKNICGKKDPRNEINNIKDSIQSI
                     EREYKKLKNDFESYSQEKIDLEKDQRGLKATLEQVQKSIIHNNRLLEGMIFESKFDNI
                     QEVEKAFRTSEERESLKDEIKIYEESKLKIVENLKRLEKRMKGRSIGEKEWLDIQRQR
                     EEASKDLEKSHQALIEKNTLVKDVKKKLEELVKTLEEKKVIDHQMALLVELENLFKGN
                     RFVEYISMGQLKYIAREASQELKKITRGRYALELDIEGNFIIRDDFNGGVRRGTKTLS
                     GGETFLTSLALALALSSQIQLKGKSPLEFFFLDEGFGTLDSDLLETVMNSLEKLHSDR
                     LSVGLISHVEELKQRIPRKLIINPPEPGVSGTIIGLEKS"
     gene            1396..2223
                     /locus_tag="VFD08_05040"
     CDS             1396..2223
                     /locus_tag="VFD08_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK10417.1"
                     /translation="MSKIQIVTDSTAYLTKDEIKEYNIKVVPLMVHFQDQDTIEGLPG
                     EFEEFFDKLKTSEDFPTTSQPSAGAFAKVFKEAIDDGKEVIAITISSKLSGTYNSALI
                     AGDMVDSDKVTVIDSLSSVANLKHMAIKAHKMAVEGKTREEIRKDIEDLKTKMGIYLT
                     VGTLEYLKKGGRLSATQAFMGTILNVKPILKVVNGVIEPVAKVRGKKKAIETIIGSIP
                     EEVKTVYIPHIFNEEEALEIKSTLENRFPNARLEITEIGPVVGGHLGPKVVGVCFTW"
     gene            complement(2286..>2474)
                     /locus_tag="VFD08_05045"
     CDS             complement(2286..>2474)
                     /locus_tag="VFD08_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10418.1"
                     /translation="NYDYLIEPPIFKDIACQMPQVIYSLIAIMAFNLFILVFHIITVA
                     LQNRKAKKQLEPIGISDV"
BASE COUNT          961 a          356 c          549 g          608 t
ORIGIN      
        1 gtaggaatag ttcagaataa agtagggata aaagaccagg gaattgaatt aaaagaagag
       61 cttaaaaaca tatgtggaaa aaaagacccc cgaaatgaaa taaataatat aaaagactct
      121 atccaatcaa ttgaaaggga atataaaaag ctaaaaaatg attttgaaag ctatagccaa
      181 gaaaagatag acttggaaaa ggatcaaagg ggattaaaag ctaccttaga acaagttcaa
      241 aagagtataa tccacaataa cagactatta gaagggatga tttttgaaag taagtttgat
      301 aatatacaag aagtggaaaa agcatttagg acctcagagg aaagggaaag cctaaaagat
      361 gaaataaaga tttatgagga aagcaaatta aaaatagttg aaaaccttaa gaggctggaa
      421 aagagaatga agggtaggtc tattggggaa aaagagtggc tagatataca aagacaaagg
      481 gaagaagcta gtaaagactt ggaaaaaagc caccaggccc taatagagaa aaatacccta
      541 gtaaaggatg ttaaaaagaa actggaggaa ttagtaaaaa ctcttgagga gaaaaaggtc
      601 atagatcacc aaatggcttt gttagttgaa ttagaaaacc tatttaaggg aaataggttt
      661 gttgaatata tatctatggg gcaattaaaa tatatagccc gggaagcatc tcaggaatta
      721 aagaaaatta ctaggggaag atatgcatta gaactggata tagaggggaa ctttattatc
      781 agagatgatt ttaatggagg agttaggcgg ggaactaaga ccctttctgg gggtgaaaca
      841 tttttaactt ccttggccct cgctctagcc ctatcatccc agatccagtt aaaaggaaag
      901 tcacctttag aattcttctt tttagatgag ggctttggta ccctagatag tgacctgtta
      961 gaaacagtta tgaatagctt ggaaaaactc cattcagata ggttaagtgt aggtctaata
     1021 agccatgtgg aggaactaaa acaaaggatt ccaagaaaac taataataaa ccctccagag
     1081 ccaggggtaa gtggaacaat tataggacta gaaaagagtt agactagact tcattatgta
     1141 aacaaatcat tgtaggggtt gggctctgcc caacccgtgt gtgggaacgt cgtccaggta
     1201 aactacccaa tcattgtagg ggttggattc tacccaaccc gtgtgtggga atgtcgtcca
     1261 ggtaaactac ccaatcattg taggggttgg gttctaccca acccgtgtag ggaacattag
     1321 tcccctaaag ggactttgaa aaatatgctt ttttatttat aatataataa taaatcaaaa
     1381 ccaaggggga caaaaatgtc aaaaatacaa attgttacag acagtacagc atatttaaca
     1441 aaggatgaaa taaaagaata caatataaaa gttgtgcctc taatggtaca ttttcaggac
     1501 caggatacta ttgaagggtt accaggagaa tttgaagaat tctttgataa gttaaagaca
     1561 tcagaggact ttcccacaac ctcccagcca tcagcaggag ccttcgctaa ggtttttaaa
     1621 gaagccatag atgatggaaa agaagtcatt gcaataacaa tatcctcaaa attaagtggt
     1681 acatataata gtgccctgat agcaggagat atggtagata gtgataaagt aaccgtgata
     1741 gactccctta gctctgtggc aaaccttaag catatggcaa taaaggccca taaaatggca
     1801 gtggaaggaa agaccaggga ggaaatcaga aaggatatag aggacctaaa aacaaaaatg
     1861 ggaatttatt tgacagttgg gaccttagaa tacctaaaaa aaggtggcag gttgtcagcc
     1921 acccaagcct ttatgggaac aatcttaaat gtaaaaccta ttttaaaagt agtaaatgga
     1981 gttatagaac cagtggcaaa agtaagggga aagaaaaaag caatagagac gataatagga
     2041 agtattcctg aagaagtaaa aacagtatat ataccccata tatttaatga ggaggaagcc
     2101 ctagaaataa agtcaaccct agaaaataga tttcccaatg caaggctaga aataactgaa
     2161 ataggaccag ttgtgggagg gcacttaggg ccaaaggtag taggagtctg cttcacctgg
     2221 tagggaagtt ttgcatactt atttggaatt tgtaggggcg accagtggtc gcctgatttt
     2281 atttactaaa catcagaaat tccaataggc tctagttgtt ttttagcttt acggttttgc
     2341 aaggcaacag tgataatatg aaatacaagt ataaataaat taaaagccat tatagctata
     2401 agagaatata tgacctgggg catttggcaa gcaatatctt taaagatggg tggttcaatt
     2461 aaataatcat aatt
//
LOCUS       DASRNA010000182         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_361465,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000182 DASRNA010000000
VERSION     DASRNA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361465"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1207
                     /gene="rocF"
                     /locus_tag="VFD08_05050"
     CDS             299..1207
                     /gene="rocF"
                     /locus_tag="VFD08_05050"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZK10419.1"
                     /translation="MNPGINPKLSIIGVPLDLGASKRGSSLGPDAIRFAGLKNRLEKI
                     GYQVQDEGNIPFHAVETGKDSTNHKNLASVVEANENLAAKVDEVMKSGRFPLVIGGDH
                     SIAIGTIKGVLKNVARLGVIWFDAHGDINTGDTSPSGNIHGMPVASLLGRGLERLSAI
                     GGSEYKLDKEKIVYIGCRDLDSGERVALKELGIHVFTMHEIDLKGIQWVMDKAMEIAS
                     KDTDGVHVSFDIDVLDPSHAPGTGTIVPGGLGYREAHFSLELVSKNVKLVSAEFVEVN
                     PILDDKNKTAEAAVALTGSLLGEWLI"
     gene            complement(1274..1771)
                     /locus_tag="VFD08_05055"
     CDS             complement(1274..1771)
                     /locus_tag="VFD08_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZK10420.1"
                     /translation="MKVYTLWTQMSQGLNQTQSNAFWQEYFAIETDIYKQLLNKKDPH
                     IQGTLEELAREYEVDNPVFAGFLDGINESILPPIELEDLEETSLIDVKVDYEKLFYNM
                     MEAKADWLYNLPIWKELLDNQKRNELRKTYLAAHTRSVEKVGRNAPCPCGSGKKYKYC
                     CINKQ"
     gene            1894..>2548
                     /locus_tag="VFD08_05060"
     CDS             1894..>2548
                     /locus_tag="VFD08_05060"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HZK10421.1"
                     /translation="MPLIGKMLIAQGGGPTAVINESLVGVIKEARRNPNVTHIYGALH
                     GIQGIAEEDFVDLAYETEGNLECIAKTPGSILLSTRVKPDEAYLMRMFEVMRHHNIRY
                     FLYIGGNDSAETVRIIKEFAQREDYDLMAVHIPKTIDNDLRENDHSPGFGSAARYVLQ
                     AFRGVEKDNMAMGGIYIGIIMGRHAGFLTAAAALAQQSEDEGPHLIYVPEIPFKLEKF
                     "
BASE COUNT          753 a          465 c          565 g          765 t
ORIGIN      
        1 cctccatgga acatccattg tgggcaatat tatgattttt gccataggcc ttaatcttct
       61 ttttgatgcc aagttatctg tagccaatat gctgccagct cttttcttag ctgccctggc
      121 aggctttttt atataaaaga aagtttgatt tggcactttt tggggttttt ctaaaaaaaa
      181 ccccaaaaca caggctggaa aagcatggta aaagtaggta gattagataa gctttccttg
      241 aaatatcgga taaatttagt tataataaag acataactaa ttagaaacgg aggagaatat
      301 gaaccctggg ataaacccga aactatctat cattggagtt cccttggatc ttggagcaag
      361 taagcgaggt tcgagcttag ggcctgatgc aatacgtttt gccggtctta aaaacagact
      421 agaaaaaatc ggctaccaag tgcaggatga aggaaatatc ccctttcatg ccgtagaaac
      481 gggtaaagac agtacaaatc acaagaatct tgcctcggtg gtagaggcca atgagaacct
      541 ggcagccaag gtggatgagg taatgaagtc cggccgattt cctctagtta ttgggggtga
      601 tcactccatt gctattggaa ccattaaggg tgtgctaaaa aatgtagcaa gactaggggt
      661 gatttggttt gatgcccatg gtgatataaa cacaggtgat acttctcctt caggaaacat
      721 tcatggtatg cccgtagcct ctcttcttgg tagaggccta gagcgcctgt ctgccatagg
      781 tgggagtgaa tataagcttg ataaagaaaa gattgtctat ataggctgta gagaccttga
      841 cagtggagag agggttgccc taaaagaact tggaattcat gtatttacta tgcatgaaat
      901 tgatcttaag gggattcagt gggttatgga taaggccatg gagattgctt ctaaggacac
      961 cgatggggta catgtttcct ttgatattga tgtactagat ccctctcacg cccctggaac
     1021 gggcaccatt gtccccggag gactaggcta cagggaggct catttttctt tagagctagt
     1081 tagtaaaaat gtcaaactag ttagtgcgga gtttgttgag gtaaatccca ttttagatga
     1141 taaaaataaa acagcagaag ctgcagtagc ccttacaggt tctcttttag gagaatggct
     1201 gatataaata ggctagtaaa caaaaaacag cgctagtcgc tgtttttttt atggtactaa
     1261 gtcctattta tttttactgt ttgtttatgc agcagtattt gtattttttt ccactgccac
     1321 agggacaggg ggcatttcta ccgacttttt caactgaacg tgtatgggct gcaagatagg
     1381 tttttcgcag ctcatttcgc ttttgattat caagaagctc cttccatatg ggtagattgt
     1441 aaagccaatc tgccttggcc tccatcatat tgtaaaagag tttttcataa tctaccttaa
     1501 catcaattag actagtctcc tcaaggtctt ctagttctat tgggggtagg atggattcat
     1561 ttattccatc aagaaatccg gcaaagacag gattatctac ctcatattct cttgctaatt
     1621 cttcaagtgt cccctggata tggggatctt ttttattaag tagctgctta taaatatctg
     1681 tttctatggc aaaatattct tgccaaaaag cgtttgactg ggtttgattt aaaccttgac
     1741 tcatttgtgt ccaaagcgtg tatactttca tactatcctc ctaaaaccca ttctaccttt
     1801 ttttgaataa aaaagaaaga caagtataga tttattcttt taacttatta tgctagaata
     1861 aggacaaagt agccctaaga aaggagagcc actatgccac tgattggaaa gatgcttatt
     1921 gcccaaggag gaggaccgac agcggtgatc aatgaatcct tggtaggcgt cataaaagaa
     1981 gcaaggcgta atcctaatgt tacccatatc tatggagcac ttcatggaat acaggggatt
     2041 gcagaggaag actttgttga tttagcctat gaaacagaag gaaatcttga gtgcatcgct
     2101 aaaacacctg gttccatttt attaagtacc cgcgttaaac cagatgaggc ctatttaatg
     2161 cgcatgtttg aagtaatgcg tcatcataat attagatatt ttttatatat agggggaaat
     2221 gattctgctg agaccgtgcg cattatcaaa gaatttgccc aaagagaaga ttatgatcta
     2281 atggcagtac atatcccaaa aaccattgac aatgacctaa gagaaaatga tcattctccg
     2341 ggctttggaa gtgctgcacg ctacgttctt caagcttttc gtggtgtgga aaaagacaat
     2401 atggccatgg gtggcattta catagggatt attatgggtc gccatgcagg gtttttaaca
     2461 gcagcagcgg cccttgccca acaatcagaa gatgaggggc ctcatttgat ttatgtgcca
     2521 gaaatcccct ttaaattaga aaagtttt
//
LOCUS       DASRNA010000183         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_361965,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000183 DASRNA010000000
VERSION     DASRNA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361965"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /gene="nadE"
                     /locus_tag="VFD08_05065"
     CDS             <1..1689
                     /gene="nadE"
                     /locus_tag="VFD08_05065"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HZK10422.1"
                     /translation="RYNGLMEVFMNHITICLAFANIQLASPDKNALVHQELIIKALDK
                     KAGLLLFPPLSLTGDGLGEFYRQPTILEGSKKALSSLAKMSSIAAFIGLPMQIEDRLF
                     SVMALIDQGEIKGYIPLSSKDKAFSDPLLFPKNLYPDKELYKLSIYLDDSFSYPSKNK
                     DVIALFKEESKKTDAPLLYLSCPESYSISGGIHSSSYILWHKEETIATDLWSDELITL
                     EVALEKGSLELFSYKPKKNNNQTFSSTPFLSPNFKGYQELLSHQAKAYGNKLLRTGSS
                     HSIIGISGGLDSTWALIATLQAHRKMNLPLDHIHPIVLPGFGSSEDTMSSALDLLDAL
                     ALKAEIISIDKAVLQHFEDLGHDPEEKNIVYENAQARERTQILMNLANKYHGLVVGTG
                     DMSEIALGWSTYNGDQMSMYGINAGLPKTLIQDLILWYAKKEGGKLEKALRHILGRPI
                     SPELLPPDPKGEILQKTEDLIGKYEIIDFILYYSLQHYPIYQIEKLMAQAFGGVSKKE
                     RKEHLARFYKRFINQQFKRTASPHGIKLTHPSLVGFEMASDLNASDFIKEIENL"
     gene            1686..2330
                     /locus_tag="VFD08_05070"
     CDS             1686..2330
                     /locus_tag="VFD08_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK10423.1"
                     /translation="MIIEHIHHSCYSILTEKHQLVFDYYYGNLELRDDKIKIFFVTHS
                     HEDHYSPKVHQLADAVVLWEGITYRDDKTYPMGVEEKLELHGINIFTSGSTDEGLSFE
                     IEVENKRLIHVGDLNNWIWPEDSKEERKQMNSDFLHYLTKFKAEPDVLFFLVDYRLKE
                     NYNLGVNQAVDYLKPKLLFPLHFSEYPEILPEYKKQIEDKVRMILPEETPFILK"
BASE COUNT          782 a          520 c          469 g          712 t
ORIGIN      
        1 aggtataatg gacttatgga ggtatttatg aatcatatta ctatttgtct tgcctttgcc
       61 aacatccaac tggcctctcc tgataagaat gctcttgttc atcaagagct cattataaaa
      121 gctcttgata agaaggcggg tctactcctt tttcctcctc ttagccttac tggggatgga
      181 ctaggagagt tttaccgcca accaaccatc cttgagggct caaaaaaagc actctcctct
      241 ctggctaaga tgtcatcaat agccgccttt attgggctac ccatgcaaat tgaggatagg
      301 ctcttttctg ttatggccct tatagatcaa ggggaaatca aaggatatat ccctctttct
      361 agtaaagata aggcctttag tgatccactt ctctttccga aaaatcttta tccagataaa
      421 gagctatata aactttctat ttatcttgat gattccttta gctatcctag taaaaataaa
      481 gatgtcattg cattatttaa agaagaaagt aaaaaaacag atgctcccct actttatctt
      541 tcttgtccag aaagctatag tatttcagga gggatccatt catccagcta tatcctgtgg
      601 cataaggaag agactatagc cacggacctt tggtctgatg aactgattac actagaagta
      661 gcccttgaaa agggttcact ggagcttttt agctataagc ccaaaaaaaa taataaccag
      721 acctttagct ccacgccctt tttatctcca aactttaaag gataccaaga gctcttatcc
      781 caccaggcca aggcctatgg aaataaatta ctgcgcaccg gttctagcca ctctattatt
      841 ggtattagtg gtggcctaga ctccacctgg gccctaattg ccactttgca ggctcatagg
      901 aaaatgaacc tgcccctaga tcatatccac cccatagtct tacccggctt tggttcttct
      961 gaagatacca tgagctcggc actggacctc ttagatgcct tggctttaaa ggcagaaatc
     1021 atatccattg ataaagccgt tctccaacac tttgaggacc taggccacga tcctgaggag
     1081 aaaaatattg tctatgaaaa tgcccaggcc agagagcgta cacaaattct tatgaacctt
     1141 gccaataaat accatggcct ggtagtggga accggagata tgagtgaaat cgcccttggt
     1201 tggtctacct ataatggaga ccagatgagt atgtatggaa tcaatgctgg ccttcctaaa
     1261 actcttattc aagaccttat tctttggtac gccaaaaaag aaggggggaa attagaaaaa
     1321 gcccttaggc atattttggg aagacccatc agcccagaac ttcttccgcc agatcccaag
     1381 ggagaaattc tgcaaaaaac cgaggactta atcggaaaat atgaaattat agattttata
     1441 ttgtattata gtcttcagca ctaccctatt tatcaaatcg agaaacttat ggcccaggcc
     1501 tttgggggtg taagcaaaaa agagaggaaa gaacatcttg ctcggtttta taaacgtttt
     1561 atcaaccaac aatttaaacg cacggccagt ccccatggga taaaactaac ccatcctagc
     1621 ctggttggct ttgaaatggc cagcgattta aatgctagcg actttataaa ggagattgaa
     1681 aatttatgat tatagagcat atacaccata gttgttactc cattcttaca gaaaaacacc
     1741 agctggtttt tgattattat tatggaaatc ttgagcttag agacgataag ataaaaatct
     1801 tttttgtcac tcatagccat gaagaccact atagtcccaa ggtacatcaa cttgccgatg
     1861 ctgtcgttct ctgggagggc attacctatc gagatgataa gacttatcct atgggggtag
     1921 aagaaaaact agaactacac ggaataaaca tttttaccag cggttctacg gatgaaggtc
     1981 tttcttttga aattgaagtt gagaataaaa gattaatcca tgtaggagat ctaaataact
     2041 ggatttggcc cgaggatagc aaggaagaaa gaaagcaaat gaatagtgac ttcctccact
     2101 atcttacgaa attcaaagca gaacccgatg tccttttctt tcttgttgat taccgcttaa
     2161 aagaaaacta taatctaggg gtaaaccaag cggtggatta cttaaaacca aagctccttt
     2221 tccccttgca ttttagtgag tatccagaga ttcttccaga atataaaaaa caaatagagg
     2281 acaaggtcag aatgattctt ccagaagaaa caccctttat attaaaatag ttaggcccga
     2341 aaaaagagag gaaacttctc ttttttattt tctactagtt tttagagttc tcaccagaag
     2401 ccccaagcta aagatcttac cctacttatg cctaatcatc tttgttattt tccagatacc
     2461 acgttaaagt gccgatatta ggt
//
LOCUS       DASRNA010000184         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_291803,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000184 DASRNA010000000
VERSION     DASRNA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291803"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..339
                     /locus_tag="VFD08_05075"
     CDS             52..339
                     /locus_tag="VFD08_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZK10424.1"
                     /translation="MKKFLEERVLKRYVSALAQDREGLLAVFSPEEDGYNFLMVSSGE
                     DINSLGKDLLKEFGGKGGGRGVNFQGWIPAEEDSDKKRERIHLWFKKSTQK"
     gene            312..767
                     /locus_tag="VFD08_05080"
     CDS             312..767
                     /locus_tag="VFD08_05080"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZK10425.1"
                     /translation="MVQKIHSEIIEPRVAETDMMGIVYHANYLIWCEVARTGLSSHLG
                     YSYSDLSQAKVLWPVRRVSVDYRRPASYGERVYIKTKITRFSGVRLIYSYEFTNDKGI
                     LLARATTEHGITDENLRVVNLFKKDPKLASCLDAYLKVCQESENTCKKQ"
     gene            752..1984
                     /locus_tag="VFD08_05085"
     CDS             752..1984
                     /locus_tag="VFD08_05085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HZK10426.1"
                     /translation="MQETVKIRDLNHSGEGVGLLGKKVCFIPGLLPGEEALIEVTENK
                     KNYARGRVKEILHFSEHRREAPCAYQGDCGGCPLMPLEYKEQFLWKKSFSERTMKKIA
                     GLDISYDDLIEADKRLYYRNKLTLFMDQGRVGLKRQASSQVIPIKECMVADKAFASFL
                     PILEGRKGFFSLVLRKADEGVMVILQGEKLEKDERFYDELEKAGAESIYFCKQKKEAR
                     SLSGDLHRLRGEKTLPYHVMGREFSLTPRSFFQIHKDQARKLYELVKDYADLKKEDRV
                     LDLYSGQGTLGAYLSPEAGEVLGVELIEEAVYYGSQRIPSTMKLLQGRVEDMELAQVD
                     LVILDPPRGGLEEGLIKKLLAMKPERIIYVSCNPVTQARDLKKLLSSYKITRAGLLDL
                     FPHTTHVESIILLQKNKA"
BASE COUNT          875 a          394 c          596 g          683 t
ORIGIN      
        1 tgacattaaa gctagaaaaa gaaaaaaaca agaagaaaga tcttctttta tttgaagaaa
       61 ttcttagagg aaagagtttt aaaaagatat gtcagtgccc tagcccagga tagagaggga
      121 cttctggcag tatttagtcc cgaagaggat ggctataatt ttttaatggt aagttctgga
      181 gaagatatca attctctagg aaaggacctt cttaaagaat ttggcggaaa aggcggaggt
      241 agaggagtta atttccaggg ctggattcca gctgaggaag atagcgataa gaaaagagaa
      301 aggattcatt tatggttcaa aaaatccact cagaaataat tgagccccgt gtagcagaaa
      361 ctgatatgat gggaattgtc tatcacgcca attatcttat ttggtgtgaa gtggcaagaa
      421 caggactatc ttctcatctg gggtatagct actcagatct tagtcaagct aaggtgcttt
      481 ggccagtgcg aagagtatct gtcgattata ggcggccggc aagttatgga gaaagagtct
      541 atataaagac aaaaatcacc cgctttagtg gtgtgcgtct tatttatagt tatgagttta
      601 caaatgataa gggaatcctt ctagctaggg ctactaccga gcacggcatt acggatgaaa
      661 acctaagggt agtgaatcta tttaaaaaag atcccaagct agcaagctgt ctagacgctt
      721 atcttaaggt ttgccaagaa agcgagaata cgtgcaagaa acagtaaaga taagagactt
      781 aaaccactcc ggagaagggg tgggtctttt gggcaaaaag gtgtgtttta ttcccgggct
      841 tctacctggg gaagaggccc taatagaagt aactgaaaat aaaaagaatt atgcaagggg
      901 aagagtaaaa gagatcctgc atttttctga gcatagaaga gaggctccct gtgcctatca
      961 aggagattgt ggaggctgtc cccttatgcc cctagaatat aaggagcagt ttctttggaa
     1021 gaagagcttt agcgaaagaa caatgaaaaa aatagcgggc cttgatataa gctatgatga
     1081 tcttattgag gcagataaaa ggctgtatta ccgcaataag ctgaccttgt ttatggacca
     1141 aggaagagtt ggattaaaaa gacaggcttc ttcccaggtc attcctataa aagaatgtat
     1201 ggtggccgat aaggcctttg ccagtttttt acctatctta gaaggtagga agggtttttt
     1261 ttctcttgtt ttacgaaagg ccgatgaagg ggtcatggtc attctccagg gagaaaaatt
     1321 agaaaaagat gagagatttt atgatgagct agaaaaggcc ggtgcagagt ccatttactt
     1381 ttgtaagcag aaaaaagagg cgagaagttt atcaggagac ctccatcgtc taaggggaga
     1441 aaaaactctt ccttatcatg ttatgggcag ggagttttct cttaccccca gaagtttctt
     1501 tcaaatacat aaagaccagg cgagaaaact ctatgagctg gtcaaagact atgctgattt
     1561 aaaaaaagaa gaccgagtct tggatttata tagtggccag ggaaccctgg gagcttattt
     1621 aagccctgag gcgggtgaag tcctgggtgt ggaactaatc gaagaagctg tttactatgg
     1681 ctcacagaga attcctagca ccatgaaact gctccagggc agggtagagg atatggagct
     1741 agcccaggta gatcttgtta tcctagatcc acctagaggt ggattagaag aaggactaat
     1801 aaagaaacta ctggcaatga agcctgaaag aatcatctat gtatcctgta atcctgtaac
     1861 ccaggctagg gatctaaaaa aactcctttc ttcctataaa ataacaagag caggactgct
     1921 agacctattt ccccatacca cccatgtgga atccataatc ctattgcaaa agaacaaagc
     1981 ataaaaatct taagatataa gcagtttatg atcattttat atttatagaa gatgaatata
     2041 acttcaagaa aaaggcactg aaaaagtgtt gtaatgtttc agtggtagtt aagctagagt
     2101 gtggaggata tacaaaaaat aataaggata aaagtgagaa tcagcaagga gtaagggctt
     2161 gctgattttt gtatataagg tgtcagactt gtcaaaagag caagtcaaga atagcgaaaa
     2221 agaaaaacct agacttgtcg aaaagataac ctaactatat agagtacagt tggattgata
     2281 ttaggggaaa gcgaaaaaac ttcgcttttt aagtagaata taagctgaaa cggtggttcg
     2341 cagtataggg tcaaggtgtg aattatggtc ctgttatgtg ttctcttttc tttgaatgca
     2401 ctttattcaa gaaaaacata tatgttatca acataattaa tatggtataa taatatatac
     2461 ttaaagtata tgtttaagca tatgaatatt tcataataaa gaatcgaggt gtttaaatgg
     2521 cctggtctta caataaacta tggaaact
//
LOCUS       DASRNA010000185         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1025850,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000185 DASRNA010000000
VERSION     DASRNA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025850"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1442
                     /locus_tag="VFD08_05090"
     CDS             339..1442
                     /locus_tag="VFD08_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HZK10427.1"
                     /translation="MSYQYWKYETLHQLCLDAFQKFGFNEKEADIIQDVLLSADLYGI
                     ESHGIQRMVRYHKTIEKGMIKIDAEPEVVFETPISIVIDAHEAMGQVVSYQAMEMAIK
                     KAKTSGVGIVSVRNSNHYGIAGYYAKMACDEGLMGFSFTNSEAIMVPTSGRMAMLGSN
                     PVACAFPAQPYDFFFDASTTVVTRGKLEIYNKLGKPLPEGWALDKNGYPSSDAPDVLA
                     NIDAKNGGGIMPLGGNSEVLGSHKGYGFGMLSEIFSSILSMGATSNYCMTGGKGRICH
                     GFIAINPAFFGDATAIKEHFSTFLEELRQSPKADGQERIYTHGEKEIEAIAERRAKGI
                     PVNDNTMVEILDLCNHLDLDFKSYFGDYLPPVK"
     gene            1478..2650
                     /locus_tag="VFD08_05095"
     CDS             1478..2650
                     /locus_tag="VFD08_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HZK10428.1"
                     /translation="MDYSKESLRLHGEWKGKIEIICTVPCENEEDLGLAYTPGVAAPC
                     LEIQKDVDKSYELTRRHNLCAVVTDGTAVLGLGDIGPEAAMPVMEGKCALFKNFGDVD
                     AFPLCIKSKDVDEFVDAVHLISGSFAGVNLEDISAPRCFEIERKLKEKCDIPIFHDDQ
                     HGTAVVTLAGLINALKVVGKKKEEVKIITSGAGAAAIAIGKLLLSAGFKDIIMCDRKG
                     AIYQGRDGLTWIKEEMAQQTNLEKKKGSLADVLVGADVFIGVSAPGLVTKEMVASMNK
                     DAIIFACANPVPEIYPDQAKLGGAKVISTGRSDFPNQINNVLAFPGIFRGTFDVRASD
                     INEEMNMAAAMALANLISDEELNEDYIIPKAFDPRVGKAVAKAVAEAARKTGVARI"
     misc_binding    2795..3018
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          948 a          589 c          746 g          930 t
ORIGIN      
        1 cataaatatg gtaatattat atacaataaa agacaagagc ctgctggtaa aaaagatata
       61 gatggcaaag aggcagctta aactctctta ggtgagcaag gacttctgag ggcttatatg
      121 tcttttctag caaggaatat attttgtctt ggtaaagttc agacttacaa gaatgtcaat
      181 atgtttcatt atcaatagga gagctcttag cccagtctaa aaggaaatat tggatattcc
      241 ccgtctgtat ctactgtgca tagttaaata ttgctataaa gattttcgct tactatgcac
      301 tagaagcaga aatcgcaatt actctaggag gcaaatttat gtcatatcaa tactggaagt
      361 atgaaactct gcatcagctc tgccttgatg cctttcaaaa gtttggattt aatgaaaagg
      421 aagctgatat catccaggat gtcttgctgt ctgctgatct ttatggcatt gaatctcatg
      481 gcattcaaag aatggttcgc taccacaaga ccattgaaaa aggaatgatt aagattgacg
      541 ccgagcctga ggtggttttt gagactccca tctcaatagt tattgacgcc catgaggcta
      601 tgggccaggt agtatcctat caggccatgg aaatggctat taaaaaggct aaaacatcag
      661 gagttggtat cgtttctgtg cgtaattcca accactatgg tattgctggc tactatgcca
      721 agatggcctg tgatgagggc ctaatgggct ttagcttcac caactccgaa gccattatgg
      781 ttcccacctc tgggcgtatg gccatgctgg gctccaaccc agtagcttgt gcctttcctg
      841 cccagcccta tgacttcttt tttgatgctt ctactacggt ggttactcgc gggaaactgg
      901 agatctataa caagctagga aaacctctgc ctgagggatg ggccctagat aaaaatggtt
      961 atccttcctc tgatgctccg gacgttcttg ctaatattga tgccaaaaat ggcggtggga
     1021 ttatgcctct aggtggaaat agcgaagtcc taggcagcca taagggatat ggctttggta
     1081 tgttatctga gatattctcc tccattttat ctatgggagc tacttctaac tactgtatga
     1141 ccgggggaaa aggacggatt tgtcatggct ttattgctat caatccagct ttttttggtg
     1201 atgctactgc catcaaagaa catttttcta cctttttaga agagttaagg cagtccccta
     1261 aagccgatgg gcaagaaaga atctacaccc atggtgaaaa agaaattgaa gcaattgctg
     1321 agcgtagggc caagggtata cctgttaatg acaataccat ggttgaaatt ctggatttat
     1381 gtaatcattt agacttagat tttaagagtt actttggcga ttacctgcct cctgtaaaat
     1441 aggatgcttt taaaagaaat gtaaatagga ggaaaatatg gattattcaa aagaatccct
     1501 tcgcctacac ggcgagtgga aggggaaaat cgaaattatt tgcacggttc cctgtgaaaa
     1561 cgaagaagac ctgggactag cctacacccc tggtgttgca gccccttgtc tggagattca
     1621 aaaggatgtg gacaagtcct atgaactgac tcgccgacat aatctttgtg ctgttgtaac
     1681 tgatggcacg gctgttttgg gcctaggaga cattggccca gaagctgcta tgcctgttat
     1741 ggagggaaaa tgtgccctct ttaagaactt tggggatgta gatgcctttc ccctatgtat
     1801 aaagtcaaag gatgttgacg aatttgtcga tgcagtgcat ctaatttctg gttcttttgc
     1861 cggtgtgaac ttagaggata tctctgcccc acgctgtttt gaaattgagc gaaaactaaa
     1921 agaaaagtgc gatattccta tatttcatga tgaccagcat gggactgctg tagttacact
     1981 agctggcctt atcaatgcac ttaaagttgt gggaaaaaag aaggaagagg taaagatcat
     2041 cacctctggt gctggggcag ctgctattgc aattggtaag ctcctactga gtgcaggctt
     2101 taaagatatt attatgtgcg atcgtaaagg agctatttac cagggccgtg atggcctaac
     2161 atggattaaa gaagaaatgg cccagcagac caatctagaa aaaaagaagg gcagcctggc
     2221 tgatgtgtta gtaggagctg acgtctttat tggtgtttct gctcctggcc tagtgacaaa
     2281 agaaatggtt gctagcatga acaaggatgc cattatcttt gcttgcgcca atcctgttcc
     2341 agagatttat cctgaccagg caaaactagg tggggcaaag gttatttcta ctggccgcag
     2401 tgactttcct aaccagatta ataatgttct agcatttcct ggtatcttcc gcggcacctt
     2461 tgatgttcgg gcttcggaca ttaacgaaga aatgaatatg gctgcagcaa tggccttggc
     2521 caatctaata agcgatgaag agttaaatga agactatatc atccctaaag cctttgaccc
     2581 tagagtgggt aaggcagttg ccaaagcggt agcagaagct gcccgtaaga ccggtgttgc
     2641 acggatttaa gagtagaaat agagggcctt gttttctcag ctaaaagaaa aagaaataag
     2701 gccctctcct tactttggaa agttagctag ccttgttttc ttaaagaaaa agagcaaaaa
     2761 gtttgacttt taatattatt agtagtaagc tagtttaaac caaatgaaaa gaagagtaac
     2821 tacaaggtag attttacaga gagactcgtt tgctgagaga gttaattgag agtgtagtga
     2881 agatggtctt ggatgggatt gttcaatatg agggcaatac ggttactccc gttatagagt
     2941 tagagtatgt tggtacttga agagaggtgt atatttatac acaaataagg tggtaacgcg
     3001 aagaaattcg tccttattgc aattcattgc agtagggact tttttaattt tatcacccta
     3061 taggaaatga ttaagacttg agaacttaat tcctccttct attttctctt ctataaaaaa
     3121 caagaaaaag gagaaagaaa tgaaaaaaca attcgttaaa ctagtttgtc tactaattgc
     3181 catcctgacc ctgggggctt gttctaatgg gaa
//
LOCUS       DASRNA010000186         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_802271,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000186 DASRNA010000000
VERSION     DASRNA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802271"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VFD08_05100"
     CDS             complement(<1..1304)
                     /locus_tag="VFD08_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZK10429.1"
                     /translation="MNYEIVRYVLAKLMRIEAALLTLPLLVGLIYKESASTLLAFVFT
                     IVILLIVSTILYNKKLEDNHFYMREAFVIVGLSWICLSFFGGLPFYFSGEIPRLVDCF
                     FETASGFTTTGSSILNDIEALSHSMLFWRSFTHLIGGMGILVFALAVLPKIPTDSLRM
                     LKAEVPGPSFGKLVSKLSVTARILYVIYISMTAVLVTFLLLGGMNLFDALIHAFGTAG
                     TGGFSNRALSVGFYDSVYFETVLGVAMLIFGMNFNLHYIFIFGAKKDILKNEELRWYL
                     LIALGGVLAIALNTRGLYNGMESLRHSFFTVSSIMTTTGFSTVNFAVWPAFSRIVLLG
                     LMCMGACAGSTAGGLKISRVVVLVKSALTEIKRARQPKRVVRLTQEGRPVSDQGVLSI
                     LNYFLLYVFIILILFLFLSLDLKDIESSLSAVLATFNNIGPGL"
     gene            complement(1307..2659)
                     /gene="trkA"
                     /locus_tag="VFD08_05105"
     CDS             complement(1307..2659)
                     /gene="trkA"
                     /locus_tag="VFD08_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HZK10430.1"
                     /translation="MNIVIVGGGKVGELLCLELSAAGNNILLIEKNADVLEALMERAD
                     ISGIVGNGASFPILMEADVAHCDVFIAVTEMDEINMISAIMANKIGANFTIARVRTPE
                     NYQSFSFVEDSLGIHMMINPELEAAKKIFTELEFPFALNVDSFANNLVSLVKVSIDSG
                     SSLDGIDLFSFRKRYSSMIVCIIERDGEVFIPRGDSVLREGDHIHVTGRPADLEKFYS
                     SLGAKHRRVKSCLIIGGGRITHYLLSMLANTDMDVKVIEHDKLKAQELAVAFPKVAVI
                     YTDGSEKSNLDEEGIHRYEAVVSLTGMDEENIVISMYASKCCVQKIITKINRTAIISY
                     LGDIGLMSIITPKRLIANRITRFVRAIENSKGSNVEALYKLADDRVESLQFRVKEQSK
                     IIGIPIKKLPLKKNMIIAYIIRRGEAFFPGGEDNIEVGDKVIVITIKDGLMDIDDILE
                     "
     gene            complement(2656..4146)
                     /gene="glpK"
                     /locus_tag="VFD08_05110"
     CDS             complement(2656..4146)
                     /gene="glpK"
                     /locus_tag="VFD08_05110"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZK10431.1"
                     /translation="MKYILSLDQGTTSSRAILFNDKGQITSVAQKEFTQIYPKEGWVE
                     HKAWEIWTSQISVARAALEEEGIALSDLKGIGITNQRETTILWDRKTGEPVYNAIVWQ
                     CRRSASYCEELKKQGHEDFLFERTGLVLDAYFSATKILWLFDHIPGLKERAKAGEIAF
                     GTVDSWILWNLTRGEVHATDVSNASRTMLFNLQELSWDKEILELLDIPEEILPQVKDS
                     SGFFGLTHPEVFGGAQIPITGIAGDQHAALFGQLCFEEGMTKNTYGTGCFMLMNTGKK
                     PVIKANGLLATVGWKIGDEICYALEGSIFIAGAAVQWLRDEMELIEDAPSSEYYAMSA
                     SESGVVVVPAFTGLGAPYWDMYARGSIFGLTRGSKKEHIIKATLESIAFQSRDVLELM
                     QKDSGIELKGLMVDGGASANNYLMQFQADLLQKQIFRPKNLESTALGVCYLAGLGAGV
                     WKSKEELKDLKIEERNFLPQKDSDWVKDKVNLWNKAVERCLGWEEK"
BASE COUNT         1309 a         1005 c          723 g         1235 t
ORIGIN      
        1 aggccaggtc caatattgtt aaaagtagcc aggaccgctg aaagagagct ttctatatcc
       61 tttaagtcca aacttaaaaa aagaaaaaga atcaagataa taaaaacata gagcaaaaaa
      121 taattaagta tagataaaac cccttgatca ctcactggcc taccctcctg ggttaggcga
      181 acaactctct tgggctgacg agctctttta atttctgtta aggcactctt aaccagtaca
      241 acaacgcgag aaatttttag tcctccggcc gttgatccag cacaagctcc catacacatc
      301 aagcccaaaa gcacaattct tgaaaaagca ggccaaactg cgaagtttac cgtactaaag
      361 ccagtggttg tcataatgga actcacagtg aaaaagctat ggcgtagact ttccattcca
      421 ttatataatc cccgagtatt tagggcaata gcaagaactc ctcctagggc gatcaataaa
      481 taccatcgta attcttcgtt tttaaggatg tcttttttag caccaaaaat aaaaatatag
      541 tgtagattaa agttcatccc aaagataagc atggccacac ccagtacggt ttcaaaataa
      601 acactatcat aaaagcccac acttagagcc ctattgctaa agccccccgt tcccgccgta
      661 ccaaaggcat gtataagtgc atcaaaaaga ttcattcctc ctagaagaag aaaagtaaca
      721 agcactgctg tcatggaaat ataaataaca tataaaattc ttgctgtaac acttagctta
      781 ctaactagtt ttccaaagct aggtccagga acctccgcct taagcatcct caaactatcg
      841 gtaggtatct tgggaagaac agccaaggca aatacaagaa tccccattcc tccaattagg
      901 tgggtgaagc tgcgccaaaa taacatggaa tgacttaggg cttctatatc attaagaata
      961 gagcttcctg tagtggtaaa accactcgct gtttcaaaaa aacaatctac taaacggggg
     1021 atttctccgg aaaaataaaa gggcaaacca ccaaaaaaac tcaaacatat ccagcttaga
     1081 cctacaatta caaaagcttc tcgcatataa aagtgattgt cctcaagctt tttattatat
     1141 agtattgtac tcacaataag aagaataaca atggtaaaaa caaaggcaag taaagtggag
     1201 gctgattctt tgtaaatcaa ccccacaagt aagggcaaag taagaagcgc tgcttctatg
     1261 cgcattaatt ttgctaagac atatcgtact atttcatagt tcataattat tccagaatgt
     1321 catcaatatc cataagtcca tctttgattg tgatgacaat gaccttatct cctacttcta
     1381 tattgtcttc tccacccgga aaaaaggcct ctcctctccg aataatataa gcaataatca
     1441 tatttttctt aagtggcaat ttcttaatgg gaattccaat tatcttggat tgctccttga
     1501 cccggaactg caaactctcc actcggtcat ctgccagttt ataaagggct tctacatttg
     1561 agcccttgga gttttctatg gcccgaacaa atcgagtgat tctatttgca attaaccgct
     1621 tgggagtaat aatactcatt aggccaatat ctcctagata actgataata gcggtgcggt
     1681 tgatcttggt tataattttt tgtacgcagc acttggaggc atacatactg attacaatat
     1741 tttcttcatc catacctgtt aggcttacca ctgcttcata gcgatggatg ccttcttcat
     1801 caagattgct tttttcactg ccatcagtgt atataacagc taccttagga aaggctaccg
     1861 ccaattcctg ggcttttagc ttatcgtgct ctatgacttt tacatccata tctgtattcg
     1921 caagcatgga aagtaaatag tgagtgattc ttccgccccc tataattaag cagcttttaa
     1981 ccctacggtg cttggctccc aaggaactat agaacttctc caggtctgcg ggtcttcctg
     2041 tgacatggat atgatctccc tctctaagga cgctatcgcc cctggggata aaaacctcac
     2101 catctctttc tataatacac actatcatgg aactatatct cttacgaaaa ctaaataagt
     2161 ctatcccatc aagagagctg cctgaatcaa tagaaacctt aacaaggctg acaagattat
     2221 tggcaaagct atccacatta agggcaaagg gaaactcaag ttcagtaaaa atctttttag
     2281 ccgcctcaag ttcaggatta atcatcatat gaatcccaag actatcttca acaaagctaa
     2341 aggattgata attctctggt gtacgtaccc tggctatggt aaagttggct ccaattttat
     2401 tggccataat agctgaaatc atattgattt catccatttc agttacagct ataaaaacat
     2461 cacagtgggc tacatcagcc tccattagga taggaaaaga tgctccattg cccacaatgc
     2521 cgctaatatc agcacgctcc ataagtgctt ctagcacatc tgcgtttttt tctataagaa
     2581 gaatgttatt tcccgctgcc gagagttcca aacacaataa ttctccgact tttcctccgc
     2641 ctacaatgac gatgttcatt tttcctccca gcctagacag cgctcgactg ctttattcca
     2701 taaattaacc ttatctttta cccagtcaga atctttttgg ggcaaaaaat ttctttcttc
     2761 tatcttaagg tcttttagtt cttccttact cttccaaact ccagcaccca gaccagccag
     2821 ataacaaact cccaaggctg tactctctag atttttgggt cgaaaaattt gtttttgcag
     2881 taaatctgcc tgaaattgca ttaaataatt attggcagaa gctcccccat caaccataag
     2941 tcctttaagc tctataccac tatctttttg cataagctcc aggacatctc tagattgaaa
     3001 ggcaatggac tccaaggtgg cctttataat atgttccttt ttactccccc gggttaggcc
     3061 aaaaatactt cccctggcat acatatccca atagggagcg ccaaggccgg taaaggcagg
     3121 taccactaca accccacttt cgctagcact catagcataa tattcgctgc tgggggcgtc
     3181 ttcaatcagt tccatttcat ccctaagcca ctggacagcc gctccagcaa taaaaatact
     3241 tccttccaga gcataacaaa tttcatcgcc gattttccag ccaactgttg ctagaagtcc
     3301 atttgcttta atcacaggtt ttttgcctgt attcataagc ataaagcagc ctgtgccata
     3361 ggtgtttttt gtcataccct cttcaaaaca caattgacca aaaagcgctg catgctgatc
     3421 tcctgctata ccagttatag gtatttgcgc tcctccgaaa acttcaggat gggttagacc
     3481 aaaaaacccc gaagaatctt tgacttgcgg caaaatctct tcaggtatat ctaatagttc
     3541 caaaatctct ttatcccagc tcaactcttg aaggttaaag agcatggttc ttgaagcatt
     3601 gcttacatct gtagcatgga cctctccccg agttaaattc catagaatcc aactatctac
     3661 tgtaccaaag gcgatctcac ctgccttggc cctttcttta agtccgggga tatgatcaaa
     3721 gagccaaaga atctttgttg cggaaaagta ggcatccagc accaaacctg tgcgctcaaa
     3781 taaaaaatct tcatggcctt gtttttttag ctcttcacaa taggaagcac ttcttcggca
     3841 ctgccaaacg atagcattat atacgggctc acccgttttt cggtcccaca aaatagtagt
     3901 ttctctttgg ttagtaatac caatcccttt aaggtctgat agggcaatgc cctcttcttc
     3961 tagagcggcc ctagccacag aaatttggct tgtccaaatt tcccaagcct tatgctccac
     4021 ccagccctcc tttggataaa tctgggtaaa ttccttttgg gctacactgg tgatttgacc
     4081 cttatcatta aacaatatag cgcgggaact tgttgttccc tgatctaaag acaatatgta
     4141 tttcattttt cacctcaact tcaatctatt ttaacatatc ttaagggata ttctagacct
     4201 atcttcgaaa aattgcatag gataaaaaaa gatttgcaag ataagtatgg acgaaataag
     4261 ggggccttct ag
//
LOCUS       DASRNA010000187         8083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_954846,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000187 DASRNA010000000
VERSION     DASRNA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954846"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VFD08_05115"
     CDS             complement(<1..444)
                     /locus_tag="VFD08_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZK10432.1"
                     /translation="MSIKGTRTEENLLKAFAGESQARSRYEMFAKIAKKEGYVQISEI
                     FKETARNEFEHAKRFFRFLEGGPVEITAMYPAGIEGTTPENLKAAAEGENEEWTDLYP
                     TAAKVAKEEGFPEVAAAFTAIAKVEYAHEARYLKLLENIEKNQVFK"
     gene            complement(505..996)
                     /locus_tag="VFD08_05120"
     CDS             complement(505..996)
                     /locus_tag="VFD08_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZK10433.1"
                     /translation="MKKELLDGLVEQINMEMQAAYIYLGMSTYLNEEDFEGFGRWMMS
                     QAQEEMEHAMRIYEYLQERGEHIELPALEKVAVNYKSVLDVFEAALAHEKLVTASIDK
                     LMGQAHDLRDFSAIGMLQWFVDEQVEEEDTFNAAIAKLKLAEGKGSPMLMLDREFGKR
                     EEA"
     gene            complement(1006..1170)
                     /locus_tag="VFD08_05125"
     CDS             complement(1006..1170)
                     /locus_tag="VFD08_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZK10434.1"
                     /translation="MQKWICTACHYIYDPALGDEEGGIAPGTAFEDIPEDWTCPVCGV
                     EKDMFELYEE"
     gene            complement(1217..1606)
                     /locus_tag="VFD08_05130"
     CDS             complement(1217..1606)
                     /locus_tag="VFD08_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HZK10435.1"
                     /translation="MAKIGEIYKCDICGNIVHVLHGGEGELVCCGEPMILQEEQKAEM
                     KNEKHVPVVEKIEGGYKVTVGSTLHPMIDAHYIEWIELQIPKQTLIAFLEPNEEPVAI
                     FKTDEKAIAAREYCNIHGLWINESPEG"
     gene            complement(1621..1785)
                     /locus_tag="VFD08_05135"
     CDS             complement(1621..1785)
                     /locus_tag="VFD08_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZK10436.1"
                     /translation="MKKWVCTVCQYVYDPALGDEDNGIAPGTSFEDLPEDWVCPDCGV
                     EKDMFEPYDE"
     gene            complement(1855..2400)
                     /locus_tag="VFD08_05140"
     CDS             complement(1855..2400)
                     /locus_tag="VFD08_05140"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZK10437.1"
                     /translation="MTKKELRRQLLSLRASLGKDKQREFSKQIWENVEKLEEFKKANK
                     IGVYLDFGSEVKTFSYLEKLTRKKEIFVPRLRGKEMDFVRFNGPEGLKAGAYGILEPI
                     EGKICPPEELELILAPGLGFTLEGGRLGYGGGYYDKYLTQTRAAVFALAFEIQILDKL
                     PIESWDYPLDGLISEKTSYRF"
     gene            complement(2397..2852)
                     /gene="mscL"
                     /locus_tag="VFD08_05145"
     CDS             complement(2397..2852)
                     /gene="mscL"
                     /locus_tag="VFD08_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZK10438.1"
                     /translation="MIKEFREFIKKGNAMDMAIAFVLGAAFTAIVSSLVDDLIMPLVS
                     FFTKGVDFSQLFLTLDGGFYESLAQAQEAGAAVFAYGNFINAVIQFLIIAFVLFLIVR
                     SLNKMRKEKEEAKQEVTTKTCEFCLSEIPLEATRCAFCTSEVAAKRKKQ"
     gene            complement(2865..3350)
                     /locus_tag="VFD08_05150"
     CDS             complement(2865..3350)
                     /locus_tag="VFD08_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HZK10439.1"
                     /translation="MLALEQSRAYLEQLTKGSFLICKNKGKVNLMTIGWGSMGYLWNR
                     FIVMVPIRTSRYSYEMLKEEHFFTISVPRKGQFSRELQYCGTKSGRDVDKVKELSLVL
                     EEGTIPVPHLKGAHFHLECKILYKEEIDPKNLDGQVDLECYPNKDYSVLFYAEIVNII
                     E"
     gene            complement(3407..4096)
                     /locus_tag="VFD08_05155"
     CDS             complement(3407..4096)
                     /locus_tag="VFD08_05155"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF004079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HZK10440.1"
                     /translation="MKIAIIGAMEEETSILLSKLEDLQEEGKNLYPFYRGFFQDKEIL
                     LSRSGVGKVNAALSTQRLIDLHRPDLVIMVGVAGAIEKLAIGDVVLATKVAHHDLNPQ
                     VLVDYQPYLDEKAQGYFTCDRELLQTAIQTAADLSLNYKVYTGPMASGEQFIDRQGRE
                     EISNTFKPLCVDMETAGMAHACYRSEVPFFALRSITDTEEESGLDHFEKNYKEARENA
                     QELLIEILKAL"
     gene            complement(4096..4677)
                     /locus_tag="VFD08_05160"
     CDS             complement(4096..4677)
                     /locus_tag="VFD08_05160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZK10441.1"
                     /translation="MKRLLLLLALLLLFVGCSEQAPEESLGEEPPAEEESSEQAQEVE
                     GPVVGIEIGDIAPDFELDTLEGESIKLSELRGQPVHLVFWSVDCTYCVQELPHVQEIY
                     NENKDDYNVLALNITIQDGKKTVEEFMAEEGYDFPVLLIEDTDQGLNTLQNYQVRGIP
                     HNVFIASDGKIAYNAAGMMTEETIQAVLKELLD"
     gene            complement(4712..4942)
                     /locus_tag="VFD08_05165"
     CDS             complement(4712..4942)
                     /locus_tag="VFD08_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZK10442.1"
                     /translation="MEKKVIIYTSNTCGYCHAAKDYLDSINVSYDERNVSTDMEARKE
                     LMQKGFMGVPVLLIDEEVIQGFDKAKIDQLLK"
     gene            5213..5755
                     /locus_tag="VFD08_05170"
     CDS             5213..5755
                     /locus_tag="VFD08_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HZK10443.1"
                     /translation="MAWRNRGHRGDLLEGLIDLSNEYYKNNGIARIDKISTPIKVVEQ
                     NSRGQITLGYFEKRSTVDYVGLCQGIPICFDAKESQLNYFPLSNFHAHQLEYMEDFKK
                     QGGISFFLLYFKKQDAFFLLPLELVNEQIKKAEEGGRKSIPYSLCKEKAIPIGFLNQY
                     LLHYLPAINVYLDMLEASKD"
     gene            complement(5752..6564)
                     /gene="rsmA"
                     /locus_tag="VFD08_05175"
     CDS             complement(5752..6564)
                     /gene="rsmA"
                     /locus_tag="VFD08_05175"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZK10444.1"
                     /translation="MYSRSRIGWIVQTMKKEKLVFRKRFGQNYLIDDNALERIVEAID
                     LSGGENVLEIGPGLGALTERLLTRDIALYAVEIDRDLGRILARDFAGENFHLLVDDIL
                     KVDPLVFPQPLVIVGNLPYYITSAIIMHFLESSLDISSMVLMMQKEVAHRLVASPGSK
                     EYGVLSVITQIYADVEKLFDVGRNCFMPTPKVDSSLVKLWPGKNQKLEEKGLQIIKAA
                     FSRRRKTLNNSLSSNFEKEEIGLALKNAGIDPTRRAESLSIDEFKSLIALWP"
     gene            complement(6557..7084)
                     /gene="rnmV"
                     /locus_tag="VFD08_05180"
     CDS             complement(6557..7084)
                     /gene="rnmV"
                     /locus_tag="VFD08_05180"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HZK10445.1"
                     /translation="MKIKEVIVVEGKDDAARIKSCVDASVIITSGYGINKNIYKRIDE
                     AYRRVGIIIFTDPDTVGARLRRMLLARYPKAKQAFILRSDGTKEGDIGVENASCKAII
                     KALEKVRSPREKASNFTMSDLYDLKLAGPGSKMHREKLMKKLGIECLSAKATLEKLNH
                     YGITQEEIYEALRDV"
     gene            complement(7081..7845)
                     /locus_tag="VFD08_05185"
     CDS             complement(7081..7845)
                     /locus_tag="VFD08_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZK10446.1"
                     /translation="MKLFDSHAHIDDRRLDNKYLAKKLKDAGIGGVIIPGVDRKGWKR
                     ILEMAKEEDMYYPTLGLHPQEASNTSPQVLEELERLVRDNPQVVAIGEIGLDLHWRKD
                     NLDKQRYIFKEQMLLAKKFDLPIIVHDRKANSQVFDLIEEVDPYKSGVIMHCYSGHVP
                     LAKAYVERGAYISLAGPVTFSNARVPKEVAAAVPLDKLLIETDSPYLTPHPHRGKRND
                     PSYVIYVAQEIARIKGISVEEVAKATFDNAKRAFKL"
     gene            complement(7855..>8083)
                     /locus_tag="VFD08_05190"
     CDS             complement(7855..>8083)
                     /locus_tag="VFD08_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZK10447.1"
                     /translation="YVLKVDLGQGQRQVVSGIKENYTKEELVGKTVVLLVNLKPIKLR
                     GVLSEGMLLAETKGKDVRLLEASLKTGSPIS"
BASE COUNT         2149 a         1861 c         1434 g         2639 t
ORIGIN      
        1 cttgaaaact tgattttttt caatattctc aagaagcttt aagtagcggg cttcgtgagc
       61 atactctacc ttggcaatgg ctgtaaaggc agcagctact tcaggaaaac cttcttcttt
      121 ggcaacttta gcagctgttg gatataaatc agtccactct tcgttttctc cctcagcagc
      181 tgcttttaag ttttctggag tagtgccttc aattccggca ggatacattg ccgtaatttc
      241 aacaggtccg ccttctagaa aacggaaaaa acgcttggca tgctcaaatt catttcgagc
      301 tgtttctttg aatatttctg aaatttgtac atagccttct ttttttgcaa tcttagcaaa
      361 catttcataa cgacttctgg cttgtgattc gccagcaaag gctttaagta aattttcttc
      421 tgttcttgtt cctttaatac tcatattgac tcctttaggt aaaaacagat gatctgcctg
      481 agcagatcat ccatatttat ttgtttatgc ttcttcgcgc ttaccaaact cgcggtctag
      541 cattagcatg ggtgaaccct tgccttcagc cagtttgagt tttgcaatag cggcgttgaa
      601 agtatcttct tcttcgactt gctcatcgac aaaccactgc aacataccaa ttgctgagaa
      661 gtctcttaag tcatgggcct ggcccataag cttatcaatg gaagcggtaa caagcttttc
      721 gtgtgcaagg gcagcttcaa aaacgtctaa gacgcttttg taatttacag caaccttttc
      781 aagagcggga agctcaatat gctctcctct ttcttgcagg tactcgtaaa tacgcatagc
      841 gtgttccatt tcttcctggg cctggctcat catccagcgg ccaaaacctt cgaagtcttc
      901 ttcatttaaa taggtagaca tacctaaata aatataggct gcttgcattt ccatattaat
      961 ttgttcaact aaaccatcta gtaattcttt cttcataaat cctccttatt cttcataaag
     1021 ttcaaacatg tctttttcta caccacagac tggacaggtc caatcttctg gaatatcttc
     1081 aaaggctgtt ccaggtgcaa tcccgccctc ttcatctccc agggctggat catagatata
     1141 gtgacatgct gtacaaatcc acttttgcat tgtatctctc ctttattagt tagttaccta
     1201 taagagggct aagttcttat ccttcagggg actcgttaat ccacaggccg tgaatattac
     1261 aatactctct tgcagcaatg gctttttcat ctgtcttaaa gattgctact ggctcttcat
     1321 ttggctctaa gaaagcaatt aatgtttgct taggaatttg tagctcaatc cactcaatat
     1381 agtgggcatc gatcatagga tgaagtgtag agcccacagt tactttataa ccaccttcga
     1441 ttttttcaac tacgggaacg tgcttttcat ttttcatttc tgctttttgt tcttcttgta
     1501 atatcattgg ttcgccacag caaaccagtt caccttctcc accatgaaga acatgaacga
     1561 tattgccaca aatgtcacac ttataaattt cgccgatttt tgccattttt tctctccttc
     1621 ttattcgtcg taaggttcga acatatcttt ttctacgcca caatcaggac aaacccaatc
     1681 ttcaggtaga tcttcaaaac tagtacctgg ggctatgccg ttatcctcat caccaagggc
     1741 cggatcgtaa acatactgac atactgtaca tacccatttt ttcataataa tctccttttc
     1801 gtattctttt atttatgttt ttacctaaag attgtttttt tcaaccccta atagctaaaa
     1861 acgatagctt gttttttcac tgattaaacc atctagggga tagtcccaac tctctatagg
     1921 taatttgtca agaatttgta tttcaaaagc tagcgcaaaa acagctgccc ttgtttgagt
     1981 aagatattta tcataatatc ctcctccata tcccagcctt cccccttcta gggtaaagcc
     2041 aaggcctgga gcaagaataa gctccaattc ttctggcgga caaatttttc cttctattgg
     2101 ttctaatata ccataggctc cggcttttaa accttcaggc ccattaaatc taacaaaatc
     2161 catttccttg ccccgcagtc ttgggacaaa gatttctttt ttcctagtta gcttttcaag
     2221 ataagaaaag gtttttactt cgcttccaaa gtccaaatag acaccaatct tattagcctt
     2281 tttaaattct tctagttttt caacattttc ccaaatttgc tttgaaaatt ctctttgctt
     2341 gtcttttccc aggctagcgc gaaggcttag gagctggcgg cgaagctctt ttttagtcat
     2401 tgcttttttc tttttgcggc cacttcactt gtgcaaaagg cgcagcgagt cgcttctagg
     2461 ggaatctcac ttaaacaaaa ttcacaggtt tttgtggtaa cttcttgctt ggcttcttct
     2521 ttttcctttc tcatcttatt aagagagcgg acaataagaa aaagaacaaa ggctataatt
     2581 agaaattgga tcactgcatt aataaaattc ccataggcaa aaacagccgc acctgcttct
     2641 tgagcctggg ccaagctttc ataaaaaccc ccatccaagg taagaaatag ctgggaaaaa
     2701 tctactccct tggtaaaaaa actaaccagg ggcattatta aatcatccac aaggctgcta
     2761 acaatggcgg taaaggccgc ccctaagaca aaggctatgg ccatatccat ggcattgcct
     2821 ttttttataa attcacgaaa ctctttaatc atcctggctc ctagttattc tattatattg
     2881 acgatttccg cataaaatag aactgagtag tccttatttg gatagcactc caaatcaacc
     2941 tgtccatcta gattttttgg atcaatctct tctttgtaga ggattttaca ctccaagtga
     3001 aagtgagctc ccttaagatg gggaactggt atggttcctt cttctagtac caaagataat
     3061 tccttgacct tatctacatc tcggccgctt tttgtaccac agtattgaag ttctctagaa
     3121 aattgacctt ttcttggaac ggagatggta aaaaaatgct cctctttaag catctcataa
     3181 ctatatcgac tggttcttat gggcaccata acaataaaac gattccataa atagcccata
     3241 ctgccccagc caatggtcat caaattaact tttcccttat ttttacaaat aaggaaactt
     3301 cccttggtta attgctcaag ataggctctt gattgctcaa gtgcaagcat agttcctcct
     3361 ttaatacata tactgcctct attatatatg aaaatccctg atttttctag agtgctttta
     3421 gtatctctat cagaagttcc tgggcatttt ctcgagcctc tttatagttt ttctcgaaat
     3481 gatctaggcc actttcttct tctgtatctg taatagagcg cagggcaaaa aaaggaactt
     3541 cagaccgata gcaggcatga gccattccgg ctgtttccat atccacacaa aggggcttaa
     3601 atgtattact aatttcttct cttccctgtc tatcaataaa ttgttctcca ctggccatgg
     3661 gccctgtata taccttataa tttagggata aatctgccgc cgtttggatg gccgtttgca
     3721 aaagctcccg atcacaagta aaatagccct gggctttttc atcaagatag ggctggtaat
     3781 caaccaggac ctggggattt aagtcatggt gggccacctt tgttgccaaa accacatctc
     3841 ctatggcgag tttttcaatg gctccagcca ctccaaccat aataaccaga tcaggcctgt
     3901 gcaggtcaat cagtcgctgg gtagagaggg ctgcatttac cttgcccact ccacttctac
     3961 taagaaggat ctccttatcc tgaaaaaagc ctcggtaaaa tgggtataag ttttttccct
     4021 cttcttgtag atcttctagc ttagaaagaa gaatagaggt ctcttcctcc attgctccaa
     4081 taattgcaat tttcattagt ctagtagctc ctttaacact gcctgaatgg tctcctctgt
     4141 catcattccc gcggcattat aggcaatctt tccatcacta gcgataaaaa cattgtgggg
     4201 aatgcctctt acctgatagt tttgaagagt atttaagccc tggtctgtat cctcaataag
     4261 aagaacaggg aagtcatagc cttcttctgc cataaattcc tctaccgttt ttttcccatc
     4321 ttggatggtt atattaagag caaggacatt ataatcgtct ttattttcat tgtaaatttc
     4381 ttgaacgtgg ggtaattctt gaacacagta ggtgcaatct acactccaaa aaacaagatg
     4441 gacgggttgt ccacgcagtt ctgagagctt gatgctttct ccctccagtg tatccagctc
     4501 aaaatctgga gcaatatcgc caatttcaat acccaccact gggccctcaa cttcttgggc
     4561 ctgctcgcta ctttcttcct ctgctggggg ttcttctcct aagctttctt cgggagcctg
     4621 ctcagaacaa cccacaaata aaagaagaag ggcaagtaat aatagtaatc ttttcatatt
     4681 tcctccctaa agtgaaaaag tcccgaagga cttatttcaa tagctgatcg atttttgctt
     4741 tatcaaagcc ttgaatgact tcttcatcga ttaagagtac tggtactccc atgaatcctt
     4801 tttgcattaa ttcttttcta gcttccatat ctgttgatac attgcgctca tcatagctta
     4861 cattgattga gtcaagatag tccttggccg cgtggcaata gccacaggta ttgcttgtgt
     4921 aaattataac tttcttttcc atttttaacc tcctaatagt taaaagctca ctgctaattt
     4981 tttacccgct atcacttgta ataaacataa taatattttc tatttttttt ctctgctagt
     5041 tctggggata acttgtgcaa aaagtcccta cttatccaca ctttccacag tttgtccaca
     5101 gaaaatagtc gtggtttccc ctaatcccct ctatttgtat catttgcgta aagactagac
     5161 tagctttcct tttcaacaaa tttcgtgtta ttatggtagc caggaggaat ttatggcatg
     5221 gagaaatcgt ggccaccgag gggacctact cgagggctta attgatctta gcaacgaata
     5281 ttacaaaaac aatggtattg ctcgtattga caaaatctct acccccatta aagttgtgga
     5341 gcaaaactct cgggggcaaa taacactggg ttattttgag aaaaggtcaa ctgtcgacta
     5401 tgtgggcttg tgtcagggca tccctatttg ttttgatgcc aaggagtccc agcttaatta
     5461 tttcccctta agtaactttc atgcccatca actggagtat atggaagatt ttaaaaaaca
     5521 gggggggatt agtttttttc ttctttactt taaaaagcag gacgcttttt ttctgctgcc
     5581 cctagagcta gtaaatgaac aaataaaaaa agctgaagaa ggcggacgca agtccatccc
     5641 ctacagctta tgtaaagaaa aagccattcc cattggcttt ttaaatcaat atttactcca
     5701 ctatctaccg gccatcaatg tgtatttaga tatgttagag gcaagtaaag actagggcca
     5761 aagtgcaatt aagcttttaa actcatctat agaaagactt tctgccctgc gcgtcggatc
     5821 aattccggcg ttttttaatg ccaggcctat ctcttctttt tcaaagttag aggataggga
     5881 attgtttagg gtttttctac gcctggaaaa tgctgcctta ataatctgca ggcccttttc
     5941 ctcaagcttt tgattttttc ctggccaaag cttaacaaga gaactgtcta ccttgggagt
     6001 aggcataaaa cagtttcttc ccacatcaaa gagtttctcc acatcagcat atatttgagt
     6061 gattacagaa agcactccat attccttact tcctgggctg gcaaccagcc tatgggctac
     6121 ttctttttgc atcatcagaa ccatggagct gatatcaaga gaagattcca aaaaatgcat
     6181 aataatagca gaagtaatat aatagggcaa gttgcccaca atcactaggg gctgaggaaa
     6241 aacaagggga tctaccttga ggatatcatc taccaaaaga tgaaaattct ctcccgcaaa
     6301 gtcccttgca agaattcttc ctaggtctct atcaatctct actgcgtaaa gagcaatatc
     6361 ccttgtaaga agacgttctg ttagggcacc caggcctggg ccaatctcta ggacattttc
     6421 tcctccagaa agatctatgg cctctacaat acgctctagt gcattatcat caatcagata
     6481 attttgccca aagcgttttc taaaaactag tttttctttt ttcattgttt gcacaatcca
     6541 gcctatacgg ctcctactat acatccctta atgcctcata gatttcttcc tgggtaatcc
     6601 cataatgatt gagtttttct agggtcgcct tggccgatag gcattctatc cctagttttt
     6661 tcattagctt ttctcggtgc attttagaac ctggtcctgc cagctttaaa tcatagaggt
     6721 cgctcatggt aaagttagaa gccttttccc ttgggcttct aaccttttca agggccttaa
     6781 tgatggcctt gcagctggca ttttccacgc caatatcccc ttcttttgtg ccatcacttc
     6841 ttagtataaa ggcctgtttt gccttggggt aacgcgctaa aagcatcctt cgaagcctgg
     6901 cacctaccgt atcggggtct gtaaagataa taatacccac ccgtctatag gcttcatcaa
     6961 ttcttttata aatgttttta ttgatgccat agccactggt tataataact gaggcgtcca
     7021 cacaagactt gattcttgcc gcatcgtctt ttccctcaac aacgataacc tcctttattt
     7081 tcataattta aaggctcttt tggcattatc aaaggttgcc ttggctactt cttctacact
     7141 gattcctttt attcttgcta tctcctgggc cacataaatc acatagctgg ggtcatttct
     7201 tttcccccta tgaggatggg gcgtaagata ggggctatcg gtttcaatta gaagcttatc
     7261 taagggcacc gctgctgcta cttccttagg gaccctggca ttactaaagg tcacggggcc
     7321 cgccagggaa atataggctc ctctttctac ataggctttt gctaagggga catggccact
     7381 atagcagtgc ataatgaccc cactcttata ggggtctacc tcttctatta gatcaaaaac
     7441 ctgagaattg gccttgcgat catgaacgat aatgggtaaa tcaaactttt tagccagcag
     7501 catctgttct ttaaatatat agcgctgctt gtctagattg tcttttctcc agtggagatc
     7561 caggcctatt tcaccaatgg ctaccacctg tggattgtct cttacaagtc tttctagctc
     7621 ctctaagact tgaggggagg tgttggaggc ttcctgggga tggagtccca aggtcggata
     7681 atacatatcc tcctccttgg ccatttcaag tatgcgcttc caccccttcc tatcaactcc
     7741 agggataata acgccgccaa tgcctgcgtc ttttagtttt ttggccagat atttattgtc
     7801 taaccttcta tcatcaatat gtgcatgtga atcaaataat ttcaatttat cctcctaact
     7861 aatagggctg cccgttttta ggctggcctc taagagtcgt acatcttttc ctttagtttc
     7921 agctaggagc ataccctcac taagaactcc tctgagtttt atcggcttta gatttactag
     7981 cagtacgacg gtttttccca caagttcttc ctttgtatag ttttctttta ttccactaac
     8041 aacttggcgc tggccttgtc ccaaatcaac ttttaataca tag
//
LOCUS       DASRNA010000188         5812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1026982,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000188 DASRNA010000000
VERSION     DASRNA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026982"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..924)
                     /locus_tag="VFD08_05195"
     CDS             complement(304..924)
                     /locus_tag="VFD08_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK10448.1"
                     /translation="MNYLINAIRILFLAVFLTLILNGKMVLWLAIYVISLPVALLFGR
                     VYCGYLCPMNTLMIPTDSLAKKMNLQTETSPKWLGSGNFGWFALIASLLIMIPFKRIL
                     GKNLPILLIWLFVSILVTIRYKTSVFHNLICPFGKLQKLFGKFAIFSERVDKEKCIGC
                     RLCEGVCPSDAIRVKEDKKALINTALCHQCTNCQQICPKKAIAYSK"
     gene            complement(1109..1633)
                     /locus_tag="VFD08_05200"
     CDS             complement(1109..1633)
                     /locus_tag="VFD08_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10449.1"
                     /translation="MDKFTWSIRVPIFKNRIIVNQLALAIGIPFGILCLVLLVIEAYY
                     GLAMVGGALVLAFVLVLLIFRGTYDVLFSLDEEGITCENQNKQKKRVGQMAAVTTLLG
                     LVAKNPTTVGTGMMAGVRIKERLLWKKVKKVKYLDRQRTIMLRAGWGESIAVFCREEN
                     YKEISLFIRQKTGI"
     gene            complement(1699..4002)
                     /locus_tag="VFD08_05205"
     CDS             complement(1699..4002)
                     /locus_tag="VFD08_05205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HZK10450.1"
                     /translation="MKNNSSLKSDFLKASRVFLLAILSLVIIFSLCACGEKAALEQEK
                     VALEQEKEIEKAKEEILTYKDTDPGSLVDSKEWGKVPINQLILVFNDEVGKAQAEKSI
                     KELGGLIVGEIDFINLYQVETQIKSEDELSALIETALSMSDIETAFPNVEIYSKDAEG
                     SPCTPLSDPLYTDPNNSAHYGAIGMENAWHILKSSGIKLNNVKVGVLDSSLYLGSGEY
                     KGKVQVSGDKTDEPEKTAQGEIVDGGLNHGTMVTNVIAADYENEGMVGIASVLGEKLS
                     VSVKNIYEGKKTEKTDLDESDITQGIIRDNGKDYAYIIKDLVYLKQQVESGSTVINCS
                     YGPAFPRDANQWISKAYEKFFKKIYETHPGVIFVAAAGNEGRDPISQGGLSGSNYFPG
                     GMKLSNLVTVGALNIDGSRADFSNYAASEDAEVTLSAPGVEMLLGTDENGQPIRASGS
                     SFSAPQVSATIALIQSISPGMEAQEIKELLVETAANSVTTDSGSKPIPEGMGKGILRV
                     DVAVLQAINKQRSKEDLSPYSMQELLDRSKVALFAETGFREYTLRASVQDAEDTSVSL
                     KIEIKGKASIEEEMIQSVQVGEEAIWNLTLEEDSVFVRVIRTDTQGCAFMTLKIYESV
                     SGIYEVKGVWDSSMLDGDGPEPLTDWRVRVEQQEDENMTITFLNDSGTILTGTYDDIS
                     GVFVGTDMNRPESFWQATWWMQDNTTITFDFDSMPMTAKGVLNWNWQEDVDELNAQQG
                     EDEEAHGALISWTKVNFDMLKLEDLIE"
     gene            complement(4200..4595)
                     /locus_tag="VFD08_05210"
     CDS             complement(4200..4595)
                     /locus_tag="VFD08_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10451.1"
                     /translation="MAFLSPQRQKEESAKVSSDVKAQGKGFLKSSAAIMQHWSNYYKK
                     YYSMTSADILAEESENYAIEYGDINKILYRSETTDTDSDGQNYGHQGKIEISLSNGNK
                     IKFTHTHHHDKKIKIMLLELFEEKLKYKK"
     gene            complement(4720..4851)
                     /locus_tag="VFD08_05215"
     CDS             complement(4720..4851)
                     /locus_tag="VFD08_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZK10452.1"
                     /translation="MVCNKCGSQIDSDSKFCPVCGRFVEEISNNPSPEVKEMDPPIW"
     gene            complement(4980..5699)
                     /locus_tag="VFD08_05220"
     CDS             complement(4980..5699)
                     /locus_tag="VFD08_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019191.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HZK10453.1"
                     /translation="MILFISRLVSGCSNPEEESLKDDDSIISEETKEKELEEKAEEVA
                     EEKLSGNPAQEKAFKSAKDFLSYLSFSKEGLIEQLEHDGHKSEDARFAVDSLNTDWLE
                     QAEKSAKSYLDYGAFSKSDLAQQLEYVGFTKEEAVHGAQNSGGDWLEQAEKVAKNYLE
                     EEAFSKIGLIEKLEDLGFTREEAVHGVEKVEGDWFEQAVKAGKNYLEYGSFSRDDLIT
                     QLEFVGFATEEAVYAVEEIGL"
BASE COUNT         1569 a         1360 c          984 g         1899 t
ORIGIN      
        1 caataacgta ttataaaaaa taccactgat ggaaaaccac aggaaagaca ggaaaataaa
       61 ggtagaatcg ggcattttca tttagcattc tttgggttct tttttttcga attatcttca
      121 ataaaagcta ctggctgaag ctggtagctt ttatcttttc tcttctctag aggccttact
      181 cttccttttt tataactttt tacgtattcc tagagtccat ctctttgtgc ctataagtag
      241 ctaaaacaaa gcccgagttc atatcagcca agaaaaaaat attgaatcaa tgggtaatac
      301 tctttacttt gaataagcaa tcgctttttt aggacatatc tgctgacagt tagtacattg
      361 gtgacacagg gcagtattga taagagcctt cttatcttct ttaactctaa tagcatcaga
      421 aggacaaact ccctcacaga gcctacatcc aatacatttt tctttatcta ctctttctga
      481 gaatatggca aacttaccaa atagtttttg aagctttcca aaggggcata ttaagttatg
      541 aaaaacagat gtcttgtatc tgattgttac aaggatagat acaaataacc atatcaaaag
      601 gatgggtaag tttttaccca atattctttt aaaaggaatc attattaaaa gactagcaat
      661 taaagcaaac caaccaaaat tgcctgagcc aagccatttt ggagaagttt ctgtctgtaa
      721 attcattttt tttgctaaag agtctgtggg aatcataagt gtattcatag ggcaaagata
      781 gccacaatat actcttccaa ataataaggc caccggaaga cttatcacat aaatagctag
      841 ccaaagtacc attttaccgt ttagtattaa ggtgagaaaa acagctaaaa acaaaatgcg
      901 tattgcatta ataaggtagt tcattttagc tcctcctaat tttcttttac tgtattaagt
      961 aaagttcttc ctagaaaggg ttaattctta agacaggatt gccagcatac taataagtcc
     1021 aatggactac taatttcata ggctagactc acaaagtttt gcctgcataa aactaccatt
     1081 atttcctgct gccagcttcc tttcaacttt atattccggt tttttggcga atgaatagcg
     1141 aaatttcttt atagttttct tccctgcaaa aaacggcaat ggattctccc cagccagcac
     1201 ggagcataat ggtcctttgg cggtccagat acttaacctt tttaactttt ttccaaagga
     1261 gccgctcctt tattctaact ccggccatca tgccagtgcc aaccgtggta gggttctttg
     1321 caactagccc cagtaaagta gtaaccgcag ccatctggcc cacacgtttt ttttgcttgt
     1381 tttggttttc acatgtaata ccctcctcat ctagcgaaaa taaaacatcg tatgtacccc
     1441 ggaatataag caagaccaat acaaaagcta aaaccaaggc tccccctacc atggccagcc
     1501 catagtaggc ttctatcacc aaaagcacca ggcataatat cccaaagggt ataccaatag
     1561 ccaaagcaag ctgattgact attatcctgt ttttaaaaat cggcacgcga attgaccaag
     1621 taaacttatc caataaagcc ctccctacta atatgttcta ttttgtgttt gtggtttcat
     1681 cttctagccc agtgatgcct actcaataag gtcttctagc ttaagcatat caaaattaac
     1741 cttggtccag gagataagtg ccccatgagc ttcctcgtcc tcgccttgct gggcatttag
     1801 ttcatcgaca tcttcctgcc agttccaatt taataccccc ttggctgtca tgggcatgct
     1861 atcaaaatca aaggtgatgg tagtgttatc ctgcatccac caagtcgctt gccaaaagct
     1921 ttctggccga ttcatgtccg tcccaacaaa aactccacta atatcatcat aagttcccgt
     1981 taagattgtc ccgctgtcat taaggaaggt aatggtcata ttttcgtcct cttgctgctc
     2041 cacacgtacc cgccaatcgg taagaggctc cggcccatca ccatctagca tactgctgtc
     2101 ccaaacaccc ttgacctcat atatgccaga aaccgactca tagatcttca gagtcataaa
     2161 ggcgcagccc tgagtatctg ttctaataac cctgacaaaa accgagtctt cctccagggt
     2221 gaggttccaa atggcctcct cgccaacttg gacgctttgt atcatttctt cctcaatact
     2281 agcctttccc ttgatttcaa tttttagcga aacacttgtg tcttcagcat cctgtacgct
     2341 tgccctaagg gtatactctc taaagcctgt ttctgcaaag agagcaacct tacttctatc
     2401 taaaagctcc tgcatgctgt agggactcaa gtcttcctta gatctctgct tgttgatagc
     2461 ctggagtaca gccacatcta ctcgtaaaat acccttaccc atcccctcag ggatgggctt
     2521 tgagccgctg tctgtagtaa ctgaattcgc agccgtctcc accagcagct cttttatttc
     2581 ttgggcctcc atgcctggac taatcgactg gataagtgct atcgtcgctg agacctgagg
     2641 tgcactaaaa ctgcttccac tcgcccttat tggttgcccg ttttcatctg taccaaggag
     2701 catttcaacc ccaggggcgc taagagttac ttcagcatcc tctgaggccg catagtttga
     2761 aaaatccgct cgactcccgt caatatttag cgccccgacc gtaaccagat tagaaagctt
     2821 cattccaccg gggaaatagt tgcttccact gagtccaccc tggcttatgg ggtctctgcc
     2881 ctcgtttccc gccgcagcaa caaagataac accagggtgt gtttcatata ttttcttaaa
     2941 gaacttctca taggccttgc ttatccattg gtttgcatcg cgcgggaagg ccggaccata
     3001 ggaacaattg attacggtag atcctgattc aacctgctgc tttaaataga ccaaatcctt
     3061 gataatatag gcatagtcct tgccattatc tcttattatc ccctgggtga tgtcgctttc
     3121 atcaaggtca gttttttccg tctttttacc ttcatatatg tttttgacac tcacagaaag
     3181 cttttccccc aaaacagagg cgattcccac catcccttcg ttctcataat cagcggctat
     3241 aacatttgtg accattgtgc catggttaag ccctccatct acaatctccc cttgggcagt
     3301 tttctcaggc tcatcggttt tgtctccgct aacctgaacc ttacccttat actcacctga
     3361 tcccaaataa agggaactat cgagtactcc cacctttaca ttgtttagct taataccact
     3421 actttttaaa atgtgccagg cattttccat gccaatggct ccgtagtggg ccgaattatt
     3481 tggatcagta taaaggggat cactaagggg agtgcagggg ctgccctctg catccttgct
     3541 ataaatttca acattaggaa aggctgtttc gatgtcgctc atggaaagag ccgtctctat
     3601 cagggccgaa agttcatctt ctgatttgat ttgtgtttca acttggtaaa gattgataaa
     3661 atcgatttct ccaacaataa gacctccaag ctccttgatt gatttttctg cttgggcttt
     3721 tccaacctca tcattaaaga ctagaattag ctggtttata ggcaccttgc cccactcttt
     3781 gctatcaaca agagagcctg ggtctgtgtc tttatatgtc agaatttctt cttttgcttt
     3841 ttctatttct ttttcctgct ctaaagctac tttttcctgc tctaaagctg ccttttcacc
     3901 acaggcacaa agactaaata ttatcaccag agataaaatt gctagtaaga aaaccctact
     3961 tgcctttaaa aagtctgatt taagactaga attgtttttc atcctaaaac cttccttata
     4021 tttaaaatag gctcttgagt gtcaagagtt tgatggaata aggttttgtc tgggttttta
     4081 gttgaaggag ctcttttctt ataaaacagt ttagtgacac tattttcttc cagcctggcc
     4141 atgatagaag taaagtccca gccttttata ataagtctct taaataggtt atgggagacc
     4201 tatttcttat attttagttt ttcctcaaaa agctctagga gcataatttt gattttttta
     4261 tcatgatgat gtgtatgagt aaacttgatt ttatttccat ttgaaagaga aatctcaatt
     4321 ttgccttgat gaccataatt ttgtccatca gagtctgtgt ctgttgtctc gcttctatag
     4381 aggatcttat taatgtcccc atattctatt gcgtaatttt ctgactcttc agccaggata
     4441 tccgctgatg tcatggagta gtatttttta tagtaatttg accagtgctg cataattgct
     4501 gcactgctct ttaagaagcc cttaccctga gcctttacat cacttgatac tttggcactc
     4561 tcctcttttt gacgctgggg agaaagaaaa gccacaagta atttatcctc cataaagata
     4621 agataagagg gttttctctt aaaaagcgac tctttgactt gtgcacttag aacccataca
     4681 accttttcgt tcttaccttc ttgattaaat gaccctaatt caccatatgg gggggtccat
     4741 ctccttaact tcgggcgatg ggttgttgga tatttcttct acaaaccttc cgcatactgg
     4801 acaaaactta ctatcagaat caatttggct tccacattta ttacagacca tcttattacc
     4861 cccttctaaa tcttgtaagg ctacttacct tcttttagtg acacatgtaa gactaggtct
     4921 attgtttcac cttctgcttt ttatagattt accatcttct tagactgttt tttacttttt
     4981 caaagtccta tttcctctac agcatatact gcttcttctg tggcaaatcc cacaaactct
     5041 aactgggtta ttaaatcatc tcttgaaaag ctgccatatt ccaaatagtt tttcccggcc
     5101 tttactgcct gttcaaacca gtcgccttct actttttcca caccatggac tgcttcttcc
     5161 ctagtaaacc ctagatcttc taacttctcg attaagccta tttttgaaaa agcttcctct
     5221 tctaaatagt ttttggccac tttttctgct tgttccaacc agtcaccccc tgaattttgc
     5281 gcaccatgga ctgcttcttc cttggtaaat cctacatact ctaattgctg ggccaagtct
     5341 gactttgaaa aagccccgta gtccaagtag cttttagccg acttttcagc ttgttcaagc
     5401 caatctgtat taagcgagtc aacagcaaat cttgcatctt cgcttttatg cccatcgtgc
     5461 tctaactgct caattaagcc ctctttagaa aaggaaagat agctcagaaa gtcctttgct
     5521 gacttgaatg ccttttcttg cgctgggttt ccagatagtt tctcctctgc tacttcctct
     5581 gctttttctt ctagctcttt ttccttggtt tcttcgctta ttatagaatc atcatccttt
     5641 aggctttctt cttctggatt agaacagcca gaaactagcc tactaataaa caagatcaaa
     5701 aaaattaaga caagtaagga ttttttatac aagagttttt tagtaccata aatagtgtaa
     5761 gaaggaatta tcctttcttg cactattttt tatataatga aagggtagag ca
//
LOCUS       DASRNA010000189         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_431168,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000189 DASRNA010000000
VERSION     DASRNA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431168"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFD08_05225"
     CDS             complement(<1..402)
                     /locus_tag="VFD08_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10454.1"
                     /translation="MKKNKTLHYGLIQSSYWLSYLPFFALSTTFLLDAGYSNIGIGLL
                     FASANLASIPLQSFLASAVDNGKIPLKGLMLTQGFVIFLVAGLVTWQALAPFYFLLLL
                     FLMSLQPFVNALSVACLHEDIPLDFGVSRGMA"
     gene            complement(389..1540)
                     /locus_tag="VFD08_05230"
     CDS             complement(389..1540)
                     /locus_tag="VFD08_05230"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK10455.1"
                     /translation="MKKNYTLSFSLYQAFFAFILLPFFAYGTYYLIHLGLSSVSIGIL
                     LALVSALSVLLQSPLASLADNDVFTIDGLISICLGLYVFSNLGLALGYFPLVFFTLAF
                     VCLNLINPLFNALNIEWNQRGHRVDFGISKGVSSLSYAFFSFVLGRLLLLASPLILPL
                     TAALGGGTLLILILKNKPVHRRKKKRKEKKDRISILKNNPNLVGVLLGICLVYANYAM
                     YNSYLINTIQALGGKESALGGAIAIAAMAEIPVMIFFTQIMRRFELRKLLVFATFFFT
                     LKSLFAYLATGMPMLYFSQFLQMFSFGLYLPTSVYYMASITSEDNRAEAQTALAGATI
                     LGRLFSSVLGGALIEYLGVSAMLLAATLMSASGTAFVYIFTRRISHEEK"
     gene            complement(1696..2673)
                     /locus_tag="VFD08_05235"
     CDS             complement(1696..2673)
                     /locus_tag="VFD08_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HZK10456.1"
                     /translation="MFKALRIHETKEGFTRSIEKMSTNELPPGDLLVQVHYSSLNYKD
                     ALSSIGNRGVTKSYPHTPGIDASGIVIESKSPLFKEGDFVVITGYDFGMNTHGGFSQI
                     ISVPASWVQKLPPGLSLKESMMLGTAGFTAAMSVAEILPYLKGGKVFVSGASGGVGSL
                     SVKILTHLGYEVSVPLRRESSRPFLESIGVKEFIPWEEIESSKKRALLSTKFDAAIDT
                     TGGDNLSYALKTTSYGGAVTVCGNAASADLNATVYPFILRGIRLIGIDSVLAESDFRK
                     TIWKKLGTDWKIPLLEEGIEELPLEELPQAMDKMLRGEHLGKVLVKVKE"
     gene            2772..3629
                     /locus_tag="VFD08_05240"
     CDS             2772..3629
                     /locus_tag="VFD08_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZK10457.1"
                     /translation="MKRKVLRSLFCLIQGDSLGFTKEKNQKDDLLIQEGLWSDNTAMV
                     IASLDAFSYFNGFSMMKNFVDIAEEGIYTARGYSLGMSETTKEALRRFQRGVHPFACG
                     GKKDSDNDNDALVRTWPLALYAMKKFDIGKEKEKALDFIDKMVACTHAHPRSLLLSRL
                     YSELLWDLLKEGTSDQLRKSAHRIKRGYAEHPEWIYMEKALSGSYKNGSEGIDTFASM
                     LDVLLCAGSFERAIRLALKMGGDTSSRAALVGSLAGLIYPDQPEDWMNIEKKEEIKSQ
                     LLGAWLKIL"
BASE COUNT         1209 a          708 c          846 g          995 t
ORIGIN      
        1 ggccatcccc ctggaaacac caaaatcgag cgggatatcc tcatgaaggc aagcaacact
       61 tagggcgtta acaaagggct gcagactcat aagaaaaagc agcagtagaa agtagaaggg
      121 agcaagggct tgccaggtaa ctagtcctgc cactaagaaa ataacaaatc cctgggtgag
      181 cataagtccc tttaaaggga tcttgccatt atctacggcg gaagctaaaa aagattgaag
      241 gggaatcgaa gcaaggttgg ccgaagcaaa aagaagccca atgccaatat tgctatagcc
      301 agcatcaagt agaaaggttg tggatagggc aaaaaaaggc aaataactta gccaataact
      361 agactgaata aggccatagt gaagggtttt atttttcttc atgactgatt ctccgagtga
      421 aaatatagac aaaagcagtt ccagaagctg acataagggt agctgctaag agcatggcag
      481 agacgccaag atattcaatc aaagcacctc ctaagaccga cgaaaaaagt ctgcccagaa
      541 tggttgcgcc agctaaggca gtttgggcct cggcccgatt atcctcagaa gtaattgacg
      601 ccatataata tacagaggtt ggtaggtata gtccaaagga aaacatttgc aaaaactgag
      661 agaaatacag catgggcatt cctgtggcta gataagcaaa gagcgattta agagtgaaaa
      721 aaaaggtagc aaaaactaga agttttctaa gctcaaatct tctcataatt tgggtgaaaa
      781 aaatcataac aggtatttct gccatagcag caatggctat agccccacct agggcggatt
      841 cttttcctcc tagagcctgg atggtgttaa taagatagct attatacata gcatagttgg
      901 catagaccaa acaaattcct aaaagaactc ccactagatt gggattgttt tttaaaatag
      961 agatcctgtc ttttttttcc ttccttttct ttttccttct atggacaggt ttatttttta
     1021 aaataaggat aagtaaagta cctcccccaa gagcagccgt cagaggaaga ataagggggc
     1081 tggctagtaa aagaaggcgg ccaagcacaa aagagaaaaa ggcatagctt aaagaagaga
     1141 cacccttgga gattccaaaa tctaccctgt ggcccctttg attccattca atgtttaaag
     1201 cattgaataa aggattgatc aagttaaggc agacaaaagc tagggtaaaa aataccaggg
     1261 gaaaataacc aagagcaaga ccgagattgg aaaaaacata tagtccaagg caaatagaaa
     1321 tgagaccatc tatagtaaaa acatcattat ctgctaaaga agccaagggg gattgtagta
     1381 atactgataa agcactgact agggcaagta aaatcccaat gctgacactg cttaggccaa
     1441 ggtggattag ataataggta ccgtaggcaa aaaagggcaa caaaatgaag gcaaaaaagg
     1501 cctgatataa ggaaaaagag agggtataat tttttttcat aatatttctc ccaaaaagta
     1561 tgtacgctta tagtataaca ggttgatagg aaaagatagc agaagcctta ggctaggcaa
     1621 aagttgtaaa ataaaagaag ggcttataag attatgcctt ataagccctt cttttatttt
     1681 ctataagcga gagttttatt cttttacctt taccagtact tttcctaggt gttcgcccct
     1741 gagcatcttg tccatggcct ggggtagttc ttctaggggt agttcttcta taccttcttc
     1801 cagtaggggg attttccaat ctgttcccaa ctttttccag atagtttttc taaaatcact
     1861 ttcagctaga actgagtcga ttccaattag gcgaatgcct ctaaggataa aggggtatac
     1921 tgttgcattt aaatctgctg aggcagcatt tccacagaca gtaacagccc cgccgtagga
     1981 agtggttttt aaggcatagg aaagattatc tccccctgta gtatcaatcg ctgcatcaaa
     2041 ctttgttgag agtagggctc ttttcttaga actttcaatt tcttcccagg gaataaattc
     2101 ctttacgcca atgctctcaa gaaagggtct ggaactttct ctgcgcagag gtacactgac
     2161 ctcatagcca agatgggtga ggattttaac agaaaggcta cctactccac ctgaggcacc
     2221 actgacaaag acctttcctc ctttaagata gggaaggatt tctgctactg acatagccgc
     2281 agtaaatcct gctgttccca gcatcattga ttcttttaga cttagtccag ggggcagctt
     2341 ttgtacccag gaagcaggaa cgcttatgat ctgggagaaa ccaccatggg tattcatgcc
     2401 aaagtcatag ccggtaataa caacaaagtc tccttcctta aaaagaggac tcttgctttc
     2461 tattacaatt cctgatgcat caataccagg ggtatgggga tagctctttg ttactccacg
     2521 gtttccgata gatgaaagtg catctttata gttgagggaa gaataatgaa cctgaactaa
     2581 gaggtcgcct gggggtaatt cattggtgga cattttttct atacttcggg taaatccttc
     2641 ttttgtctcg tgaatacgta atgctttaaa cattttgtct cctttcttta ggactatcat
     2701 atcatgaaaa agcttttatg tgagctagga gaagggatat aaatctgtta tgatatatag
     2761 ggaggaaagc catgaaaaga aaagttttac gcagtttatt ttgtttaatc cagggagata
     2821 gcctgggttt taccaaggaa aaaaaccaaa aagacgacct tctgattcaa gaaggactat
     2881 ggagtgataa tactgctatg gttattgcaa gccttgatgc cttttcttat tttaacggtt
     2941 tttccatgat gaaaaatttt gtggacatag cggaggaagg tatctataca gccagaggtt
     3001 atagtcttgg catgtcagag accacaaaag aagccctaag acgttttcaa aggggagtgc
     3061 atccttttgc ctgtggagga aaaaaagact ccgacaatga caatgatgcc cttgtaagaa
     3121 cctggcccct ggctctttat gctatgaaaa agtttgatat tggtaaagaa aaagaaaagg
     3181 ccctagattt tattgataag atggttgctt gtactcacgc ccatcctaga agccttcttc
     3241 ttagccgtct ttacagcgaa ttgttatggg atctgcttaa agagggaaca agtgaccagc
     3301 tgagaaagtc tgcccatcgt ataaagagag gttacgcaga gcatccagaa tggatttata
     3361 tggaaaaggc cctttcaggg tcttataaaa atggaagtga ggggatagat acctttgcct
     3421 ccatgttaga tgtccttctt tgtgctggta gctttgaaag agcaataagg cttgccctta
     3481 agatgggagg cgatactagt agtcgagccg ccctagtggg aagtttagcc gggcttattt
     3541 acccggatca accagaagac tggatgaata tagaaaagaa agaggagatt aagagtcagc
     3601 tcttaggagc ctggctcaaa atactataat ggaaatttta gcccctgttg gtaaaaaaga
     3661 aaacttaatt gctgcaatac aggcgggtgc cgatgctgtg tgacgatgca ctatagcttc
     3721 cctatcggtg tatctaccgt aggtaggact cttacccc
//
LOCUS       DASRNA010000190         6082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_431234,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000190 DASRNA010000000
VERSION     DASRNA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431234"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VFD08_05245"
     CDS             complement(<1..173)
                     /locus_tag="VFD08_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HZK10458.1"
                     /translation="MLFDHEKAERVVTFINNLKHTKGVWRGVPFDLLPWQDKIVRDIF
                     GTVKEDGYRQYNTA"
     gene            complement(173..646)
                     /locus_tag="VFD08_05250"
     CDS             complement(173..646)
                     /locus_tag="VFD08_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HZK10459.1"
                     /translation="MATRGRKPKPTALKVLEGNPGKRPLNNKEPQPEKKAPRCPSWLE
                     PEAKKEWKRMAKTLEAIGVLTQIDKAAFAGYCQAYARWKEAEEFLSKHGTIFKTPSGY
                     IQQVPQVSIAQTYLKVMKDFCSEFGLTPSARTRIRVNTVDTPTDDPMEELLRVSK"
     gene            complement(708..929)
                     /locus_tag="VFD08_05255"
     CDS             complement(708..929)
                     /locus_tag="VFD08_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10460.1"
                     /translation="MTGRQLKIMMEGLITTVVEKICVLGWEDAKEDVEKLVDLVEDLE
                     MFWDSDGRLTETDWVAEILMAIENAGGVV"
     gene            complement(995..1135)
                     /locus_tag="VFD08_05260"
     CDS             complement(995..1135)
                     /locus_tag="VFD08_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10461.1"
                     /translation="MSMFNEDCICLDCKEKERQDKDYEKAVKAEHEAVKRGNYNFPGI
                     RK"
     gene            complement(1361..1549)
                     /locus_tag="VFD08_05265"
     CDS             complement(1361..1549)
                     /locus_tag="VFD08_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10462.1"
                     /translation="MTKQKCKLIGEDGNIFNLMGIASRTLKRAGLEEEAKEMVDRIFK
                     SESYDRALQIIMDYVEVE"
     gene            complement(1550..2299)
                     /locus_tag="VFD08_05270"
     CDS             complement(1550..2299)
                     /locus_tag="VFD08_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10463.1"
                     /translation="MTRKELVHKLAEHLEVAPAYLGAPSFAYQVGDYTVDRHSNILDT
                     QGQEVELEELLANMVEEDYMEVEATQETASETEEPVVLEIGLPLEDHQGKSLRNLIHT
                     IYSKQLLIKKALGLETDLVSEEAITALAQEPMASLDHFRKAMEDITCPGIDFDFEKET
                     ITFKLGQDGDDPDKVEAATQLLALINKSARGLKRNASAKVKSTDNEKYTFRTWLLRLG
                     MIGNEYKTARKVLLKHLSGNSAFRKQAKEEA"
     gene            complement(2429..2896)
                     /locus_tag="VFD08_05275"
     CDS             complement(2429..2896)
                     /locus_tag="VFD08_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10464.1"
                     /translation="MKKKERFIATYNKVYEIAGRWEGSIVLSPVDTQDDQCLLYTAGE
                     MKELLQTGGLQKETEKKKALFGRKIASLEDLKELTARALAEGKTGESYTVTSEVELSE
                     AEFRKFAEDFFQDQPWIDPEASCIRVTNVETGEKVLIDPQGYTYPRYTALELK"
     gene            complement(3036..4268)
                     /locus_tag="VFD08_05280"
     CDS             complement(3036..4268)
                     /locus_tag="VFD08_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK10465.1"
                     /translation="MDIQKIPIEKINPAKYNPRKDLKPGDPEYEKLKKSIETFGYVEP
                     IVWNKQTGNIVGGHQRLKILQHQGHTEIECVVVNLDETEEKALNVALNKVSGEWDLPK
                     LADLISELDNGIFDISLTGFDAAEIEDLFSKVHDKDVKEDDFDVDEALKEPAISKPGD
                     LWLLGRHRLLCGDSTKAETYEKLLDGKKANLVVTDPPYNVNFTAGKENERLIKNDNQK
                     DQEFYQFLLAAFKNIYHALADGGALYVFHADTEGLNFRNAVREVGFHLSGVCIWVKDS
                     LVLGRSDYQWQHEPIIYGWKPTGRHRWYADRKQTTVWQFDRPKKSEQHPTMKPVALCA
                     YPIQNSSAPNGIVLDPFSGSFSTGIACEQTDRICYAVELDEKYVDVGVKRYIEYMGSD
                     KEVFLIRDGKKQAYKACF"
     gene            complement(4470..4844)
                     /locus_tag="VFD08_05285"
     CDS             complement(4470..4844)
                     /locus_tag="VFD08_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZK10466.1"
                     /translation="MPRKPKKPCAHPSCPELTEGRYCEKHQREMNREYNKSSRPYKKL
                     YNSSHWQRLRKHVLAKQPLCVICLKEKRITPATVVDHIKPHKGDEKLFFDINNLQPLC
                     KSCHDRKTAKEDDRWRRKVYTY"
     gene            5089..6006
                     /locus_tag="VFD08_05290"
     CDS             5089..6006
                     /locus_tag="VFD08_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HZK10467.1"
                     /translation="MDSVKPFLPIDGQIELLKSRGLNMDDTQHDIAKQFLLNNNYYRI
                     SGYSLTLRKNDSFFNNASLDMLMQIYEADRHMRHIILLTAEAVEIRIKSMVAYYHSKK
                     YGPLGYLDVNNFACLQDGKIDLQAVSRYSQIIRKAKRQKDNLKYSELFIRHHKENKND
                     ILPFWVYVEILTISDISKLYTLLDKELQKTIAGLLGFKFSNNNRVLENLLHCMSVLRN
                     ICAHGGRLYNRLFTIKPWLSTKEKNLLRKEDGNIIFNKLFSYILVLKSITLSQDFKLL
                     IEYINSIHKQYPLVDFRHYGFPDNWQEIL"
BASE COUNT         1533 a         1435 c         1098 g         2016 t
ORIGIN      
        1 gccgtattat attgccggta gccatcttct tttacagtac caaaaatatc ccgaacaatc
       61 ttatcctgcc agggcagtaa atcaaagggc acaccccgcc aaactccctt agtgtgtttt
      121 aagttgttga tgaaggttac cacccgctct gccttttcat gatcaaagag cattatttgc
      181 tcacccttaa tagttcctcc atgggatcat ctgttggtgt gtcaacagta ttcaccctga
      241 ttctggttct agcagaaggg gtaaggccaa attcagagca aaaatccttc atcaccttaa
      301 gataagtttg ggcaatggat acctggggca cttgctgaat atatccggaa ggggttttaa
      361 agatagtgcc gtgtttagat aaaaactctt ctgcttcctt ccatctagca taggcctggc
      421 agtaaccggc aaaggcagcc ttatctattt gagttaaaac cccaatagcc tctaaagtct
      481 tagccatccg cttccattcc ttctttgctt ccggctccag ccatgacggg cagcggggtg
      541 ccttcttttc tggctggggt tctttattgt ttaacggcct tttgccgggg ttaccttcca
      601 aaactttaag tgcagtcggt ttgggttttc ttccccgtgt cgccatagct tttcacctcc
      661 aattgaaaaa aggcccgaag gcctttagtt ccatttccta ctttttacta taccactccg
      721 ccggcatttt ctattgccat gagtatttcg gctacccagt ccgtctctgt taatcttcca
      781 tcggaatccc agaacatctc taaatcctcc accagatcca ctagcttttc tacatcctct
      841 ttagcatctt cccaccccag cacacaaatc ttttctacta cggtagtaat caaaccttcc
      901 atcataattt ttagttgtcg ccctgtcacc cttttcacct ccctgtggtt ttggtatgtc
      961 tatacatcac tcaacaggga ggtaatagca agggttattt tctaatcccc ggaaaattat
     1021 aattgcctct ctttaccgct tcatgttcag ccttcaccgc tttttcgtaa tccttatctt
     1081 gcctttcttt ttccttgcaa tccaggcaga tgcagtcctc gttaaacatt gacattattc
     1141 ggcccttcct taaatccttg ccgcatctat cacagtgctt ttgtgtaaag aatttgtcca
     1201 ctttgagcac cccctaactt tttttatggg tttgacaggc ttgccctgtt tggctttatt
     1261 atcaccacta aaccctttgt ccctatttaa gttttaactc gccgctgtgg ccaacagggg
     1321 gccgaagcaa ccccctcttt tggttatttc cgtagccttc ttactccact tccacataat
     1381 ccataattat ttgtagagcc ctatcatagc tttctgattt gaaaatcctg tctaccattt
     1441 ctttagcttc ctcttcaagc ccagcccttt ttaaggttct gctggcgatg cccatcaggt
     1501 taaagatgtt gccgtcttct ccgataagtt tgcatttttg tttagtcatt tacgcttcct
     1561 ccttggcctg ttttctaaaa gcgctgttgc cagagaggtg tttaagcaat acctttctag
     1621 cggttttgta ttcattgcca atcatcccca agcgtaaaag ccaagttcta aaagtgtatt
     1681 tttcgttatc ggtagatttg accttagccg aggcgtttct ttttaatccc cgagcacttt
     1741 tgtttatcag agctaatagt tgggtggccg cttctacctt atctgggtcg tctccatcct
     1801 ggcccagctt aaaggtaata gtttcttttt caaaatcaaa gtcaatgcct gggcaggtaa
     1861 tgtcttccat ggcttttcgg aaatggtcca agcttgccat tggttcttga gctaaggcag
     1921 taatggcttc ttcgcttaca agatcggttt caaggcccag ggctttttta attaaaagct
     1981 gcttgctgta gattgtgtgg attaagttac gcaggctttt cccttgatgg tcttccagcg
     2041 gtagtcctat ttccaagaca acaggctctt ctgtttcaga agcagtttct tgagtagctt
     2101 ccacttccat ataatcctct tctaccatgt ttgccagcaa ctcctccagc tctacttcct
     2161 gcccttgggt gtctagaatg ttgctgtgcc ggtcaacggt gtaatctcct acctggtagg
     2221 caaagcttgg tgctcctaga taagcaggag ccacttccaa atgctctgcc agtttgtgta
     2281 caagttcttt tctagtcatt ttcaatccct cctgtggttt ttggtatgtc tatacatcac
     2341 ttataaccac agataagtca agggttattg cacaaggata ttggtttatc tgtaactaaa
     2401 aaaagcaggc ttttaaacct gctctttact attttagctc caaggcagta tacctagggt
     2461 aagtgtagcc ttgtgggtct attaaaacct tctccccggt ttctacattg gtaaccctaa
     2521 tgcagcttgc ttccgggtca atccaaggtt ggtcttgaaa gaagtcttcc gcaaactttc
     2581 taaattctgc ttcgcttaat tctacttcgc tggttactgt ataactttct cccgttttcc
     2641 cttccgctag tgctctagct gttagttctt ttaaatcctc gagactagca atctttctac
     2701 caaatagcgc tttctttttc tccgtttctt tttgaagacc accggtttga agaagctctt
     2761 tcatttcacc tgctgtgtaa agcaggcact ggtcatcttg ggtatccacc ggagaaagga
     2821 caatggaccc ctcccatctt cctgcaattt cataaacttt gttgtaagtt gctataaagc
     2881 gttctttctt tttcaccttg cttacctcct gtgtttttgg tagtctatat atcactttta
     2941 aacgcagata agtcaagggt tatggggcat cttcttttat cttttgcact taaaggactg
     3001 cttttgaaaa cgaatgcagg ccgtaaggtg cgtaattaaa aacaggcttt ataggcctgc
     3061 ttcttcccat ctcgtattag aaaaacctct ttatcagagc ccatgtattc aatgtatctt
     3121 tttaccccta catctacata tttttcatcg agttcaacag cgtagcaaat gcgatctgtt
     3181 tgttcacaag caatccccgt tgaaaagctt cccgaaaagg gatcgagaac aatgccattt
     3241 ggagcactac tgttttgtat aggataagca caaagtgcca ccggcttcat tgtcgggtgt
     3301 tgctcggatt tcttgggtcg gtcaaactgc cataccgttg tttgcttacg gtctgcatac
     3361 catctgtgcc taccggtagg tttccagcca tagattatgg gctcgtgttg ccactggtaa
     3421 tcgctcctac ctagtaccaa agaatcctta acccaaatac agaccccgga aagatgaaag
     3481 ccaacctccc gcacagcgtt tctaaaatta agcccttccg tgtcagcatg gaaaacgtat
     3541 agagccccac catcagcaag agcatggtaa atattcttaa aagctgctag tagaaactga
     3601 taaaattcct ggtccttctg gttatcgttt ttaataagac gttcattttc cttaccagcg
     3661 gtgaaattga cattataagg cgggtctgtt actaccagat tggccttctt accatccaat
     3721 aatttttcat aggtttccgc tttcgtgctg tcaccacaaa gtagccgatg acgacccaac
     3781 aaccagaggt ctcctggctt actaatagcc ggttctttca aagcttcatc tacatcaaag
     3841 tcatcttctt taacatcttt atcgtggact ttagaaaaca aatcttctat ttcagcggca
     3901 tcaaatccag ttaaactaat atcgaatatt ccattgtcca gctcgctgat taaatccgcc
     3961 agctttggta aatcccattc accactgact ttatttaggg ccacatttaa ggccttctct
     4021 tctgtttcat ccagattcac taccacacac tcaatctccg tatgaccctg atgttgtaat
     4081 atttttaatc tctggtgacc accgacaatg tttcccgtct gtttattcca gacaatgggt
     4141 tccacatagc caaaggtttc tatggacttt ttaagctttt catattccgg atctcctggc
     4201 tttaaatctt ttctgggatt gtatttagct gggtttattt tttctattgg tatcttctgt
     4261 atgtccaaaa atagccccct ccttttcctc cttgttttta acctcttttg aaactaaaaa
     4321 atcctgtaaa attgagcttt tgccgcctat acccccaccc tcgaattttg cgaattcttg
     4381 cgtgagggcc cgccgccgtt ctgggctagc ctcgttttag aaaattagaa cccccctacc
     4441 cttgagcttc atctgctaca aaacctgtat cagtaggtat aaacttttct cctccagcga
     4501 tcatcttctt tggcggtctt cctatcgtgg cagcttttac agagaggttg taggttattg
     4561 atatcaaaga atagcttttc atcacctttg tgaggtttta tatgatccac caccgttgcc
     4621 ggagtgattc ttttttcctt caagcatatt acacaaagag gttgctttgc taaaacatgc
     4681 ttccgtagtc tttgccagtg actggagtta tagagtttct tataaggcct actgcttttg
     4741 ttgtattccc tgttcatttc cctttggtgt ttttcacaat accttccctc agttaattca
     4801 ggacaactgg gatgagcgca gggcttcttt ggttttctgg gcatttgctt tcaactccta
     4861 gtttgggcat agaaaaagcc ctagaaattc tctatggctc ttaggtattg tgtttcggtg
     4921 ttataatgtt agatattatc ccccaaactt ttttagtttt ttaccatttt ccagttccat
     4981 ccattgacaa cgtgcctacc acaatgtatt ataaagataa caaatcccac aggcctctgt
     5041 cttagacatg cacacttcgt gggctttttg tgttaggggg aggggactat ggattcagta
     5101 aagccttttc tgcccattga tggccaaatt gagctattaa aaagtcgtgg gttaaatatg
     5161 gacgatacac aacatgacat cgctaagcaa tttcttttaa ataacaacta ttaccgcata
     5221 agcggttatt ctttaacact gcgcaaaaac gattcgtttt tcaacaatgc atcattagat
     5281 atgctgatgc aaatatatga agcagatcgt catatgcgcc acataatact attaactgct
     5341 gaagcagtag aaatccgtat aaagtcaatg gtcgcatatt accatagtaa aaaatacggc
     5401 cccttggggt acctagacgt gaacaatttt gcttgtttac aagatggcaa aatcgacttg
     5461 caagcggtat cccgttattc acagataata cgtaaagcta aacgccaaaa agataaccta
     5521 aagtattccg agttatttat aagacatcat aaggaaaaca agaatgacat tttgccgttt
     5581 tgggtttacg tcgaaatcct aacaatatcc gacatctcca agctatacac cttattagat
     5641 aaagaactgc aaaaaacaat agcaggctta ttaggtttta agttttccaa taataaccga
     5701 gtcttggaaa atctattgca ttgcatgtct gtattaagaa atatctgtgc ccacggcggt
     5761 cgtctttata atcgtctatt tacaataaaa ccatggctat caacgaaaga aaaaaatcta
     5821 ctaaggaaag aggatgggaa tattattttt aacaagctct tttcttatat cttagttctt
     5881 aagtccataa cactctcgca agacttcaaa ttgcttattg agtatataaa ctcaatacat
     5941 aaacagtatc cgctggttga ttttaggcat tacggtttcc cggataattg gcaagaaatc
     6001 ttgtaatcaa aaagctgttt tagcatatac taataaataa cagatcctac gagcttattt
     6061 ataagcgcaa tactgtaggc ca
//
LOCUS       DASRNA010000191        16180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_728240,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000191 DASRNA010000000
VERSION     DASRNA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16180
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728240"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VFD08_05295"
     CDS             <1..1446
                     /locus_tag="VFD08_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HZK10468.1"
                     /translation="KLYGNMATTLGVHPVLMVKAMFRYRWMVSYLTAANMIDRHTIGI
                     KGKELRYIHEEFYSIVNNSINTIKQIIERDENLNPNSKKAAKLRENTVMFDEMTPTLI
                     MAGFPTLDWIDVAMFVIGLPGEIDQIANIYYIDAIEHFGLAPDVCPLPAAEVGAAVVD
                     DYPRVGKAFITSSMPCDGSIGQTTFMSRYFKDIPVYQITPPQRFLEPEVQNYAVKNLE
                     GCIEFIEDNFDVKWDWDAFWEGCQMYNQSSQYAINKWDVNTTPYPQVKGSALALARQY
                     QYQGAGCLDPFMLETNAKVDKMMKKGYEEDKKNDANKPKYRAIVWACPAHYYTNFTYW
                     TRHCWGVECLIDMEVMLSYHKIKIGDERQAMIDLANSYERMMMRSHTNGGYVNSLDEC
                     WKMCEKMDVNIVIMYDHVSCKNVGGLHGLYEDQARERGIHLIWVPHDLMDPRTVSRRA
                     MRDAFNNYMINVFNEKPLDPTLVDYPDELTW"
     gene            1471..2280
                     /locus_tag="VFD08_05300"
     CDS             1471..2280
                     /locus_tag="VFD08_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZK10469.1"
                     /translation="MKYFGGCDVGSTYTKAIILDQDGNILSDVTIKSKINSEESAELA
                     MEKVIEKTKGIDKLEDLTYLIGTGYGRNKVPQAQENISEISCHAMGVHVTNPDVKAII
                     DIGGQDVKGIAVDDDGTVKTFAMNDKCAAGTGRFFEAMARAFELSLDDFSKLSLGAKN
                     AIPITAQCVVFAESEVITLVGEGKPRDEIAAGIQQSVAKRCFVMAKKAGAKDSITLTG
                     GCAKNIGLKKAIENVLRIKVIDLDVDPQLMGALGAAEFARQKGLDIYGYGA"
     gene            2295..2468
                     /locus_tag="VFD08_05305"
     CDS             2295..2468
                     /locus_tag="VFD08_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10470.1"
                     /translation="MKKSSKKTLFKILLGFVVLLSGLFVVYITNLDGKMIEQTYNFLI
                     KYHDQRDDIVEAL"
     gene            2542..3243
                     /locus_tag="VFD08_05310"
     CDS             2542..3243
                     /locus_tag="VFD08_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10471.1"
                     /translation="MKELAVNPNVKWNSLEHNEFLMGNEVAFELGDPFEPCTVYNCST
                     SGGIFVPEDRISLIGKDLPELKGNTNFSRITFLDIDDVEDPNKAYTRIKRLEFERFKV
                     IPEGYMILSSSVENKEQVRVSKKAIKNGLDFSTIGNLFINRYKKVEGVNNVWMIFIVG
                     DYPFIPELVKLSGEVDEITNAFEHVLRDIITDCSICPLQTICDDVEALREQHFQALQE
                     KKEKKGKKRKKRKKR"
     gene            3249..5507
                     /locus_tag="VFD08_05315"
     CDS             3249..5507
                     /locus_tag="VFD08_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK10472.1"
                     /translation="MKKDPLVQKLVDSYGDWFIDSPQDARAMTGELYPFETMFSPISI
                     NRTKIKNRIVMAPMGNLQMCEENGRPNQKMIQYFTERAKGGCGLLTTGLIPISHGIDP
                     TVTEPGGLSYFPRIDRSRTNLVGWRELAQSVQAYGSKIFIQLTPGLGRVGPPICLPTQ
                     LKFPVSSSFNKNFYIPGVPCLRLSDFKLNKIIRNTAQAAIDAKHQGIDGVYLHGHEGY
                     LLEQMTNPAFNRRKLGKYADPHRFGVDLVKGVRESVGPFYPIMYRIDLSLALNETYED
                     IENMPTLKKYTNGRTIADTLKFMEALVEAGVDCFDVDLGCYDNWWLPHPPGSMPAGCF
                     TSVSEAAVDHFRKNNIKTNLGNDVVVVAVGKLGYPDLAEEVLRDNKADMVMLGRQLLA
                     DPYWPEKCYNNKIDDIRPCIGCQEGCINEFVEGGHIQCAVNARTGFEDKYQIPAKVEK
                     PKKIAVVGAGPAGCVFALESAKKGHNVTIFEAADQIGGNLIPGGTPMIKFDVKNYSDY
                     LKVQLAKAEKNLKLKLKLNTKVEIEDLKKGKFDTVVVAVGGADIVPPFPGLEDTPHAL
                     ATDALIDSSFLEGKSKIAIIGGGVVGIETAYKLAFEENKCVDVIEMLDDFMVGVCTAN
                     RGHLLNYMRLNDRVTLHNGCKVLGFENGKVLTEQKTCKHLPDPYNSWSPLLPENVPNP
                     LAKPTGTAAVKKEVAADYVLLALGYRAEADIYYDAIKERVAPEIYNVGDSFKPAKIIN
                     AVRGAYALAMEV"
     gene            5698..6891
                     /locus_tag="VFD08_05320"
     CDS             5698..6891
                     /locus_tag="VFD08_05320"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HZK10473.1"
                     /translation="MKLVVLGGAGSQALYGIRNLISQGSVFSEVVISSRNIEKNKEIV
                     KELNSPLVRAAQVDVSDKEALYELIKDCDVVANCTGPYHILAYDIIETTIKAGKDYID
                     FCDDIEAFNEIFESELPKKAKEKGLSMIMGLGASPGFLSILACNAEKRYFDSLDEAIF
                     YFATDEREPGGPAVMAHLLECVHNAPYIEEGELKNGPSFREEWEFDFGSTFGKRTVTR
                     IGHPEVFTFPRYSPDIKDVSVRFSLKPISAYEGFKKMSLSGLTSDEKLSINGNPVSPR
                     DFALAVLAVQKAGKKEMTKEELQELLKEVTACAATELHGKKDGKSVSYIGRLEGNMAP
                     LTAIPLIVGAEMLAKKEIAKKGILVVEEAIEETEKFIDETARRIRGDGFPFVIKEEFT
                     TRKEY"
     gene            7039..7335
                     /locus_tag="VFD08_05325"
     CDS             7039..7335
                     /locus_tag="VFD08_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10474.1"
                     /translation="MKKSKENLIGRLIIVISIILTIIGYVFLPKDLIVQVTPSGEARY
                     SLPKILALSIPFAISTLFSILYMQGEPKFRRKNLILSILGIVVFIFIFFANYGG"
     gene            7528..8349
                     /locus_tag="VFD08_05330"
     CDS             7528..8349
                     /locus_tag="VFD08_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZK10475.1"
                     /translation="MSRMIGVNTNCYHGYTIEEALEGIAKAGFHYVELTATKGWTEHV
                     FPDQSFEKLVKIRNKIKELDLHPFALSGHTNLMDPERIPDFVMNMELGAFFGCQYLVS
                     SVGEAHLEDKAHATNEEVAGAVRTFLPYLEERDMKLVLEVHGDHGTGRILKKIVELVD
                     SPRVMINYDTANVLFFSDAHMEEDLDFSLDKIGFFHLKDKAGGRKEWNFPALGKGYVD
                     FPMVFKKLEEAKNKSPFSIEIEFTDKGPKDLEEINQAVKDSYDYLRSQGFSMEKI"
     gene            8379..9548
                     /locus_tag="VFD08_05335"
     CDS             8379..9548
                     /locus_tag="VFD08_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZK10476.1"
                     /translation="MKTYEKEMDPLKKIRAGIIGAGFIGAAHIEALRRLGNVEVVALA
                     KTKGARQRAKELFVAKGYKDYREMIDKENLDAVHICTPNYLHYEMAMYAMKKGLAVMM
                     EKPFTVSLEEAQKLAKYARDNNIVGGVNHTLRMFPQVMQMKELMQKGEVGDVFAVHGC
                     YLQDWLYLDTDWNWRLEADVNGKTRAFSDIGSHWIDMVENIIGQRAVELLAEFRIVHK
                     KRKKPLKEIETFSAFAPRAEDYEEKTIETEDWCTILFKFEKGAIGCVNVSQVTAGRKN
                     QQVIEISGSKCSLKWDSEDSNELWIGRREAYNQKVPKDPSLVEPKSAELMGYPGGHVE
                     GFPDTFKMQFKKFYEAIEKKDCENVDFATFEDGVREMLLNDKVYESASKRAWVKI"
     gene            9561..10472
                     /locus_tag="VFD08_05340"
     CDS             9561..10472
                     /locus_tag="VFD08_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZK10477.1"
                     /translation="MMKMGLLTAILPDLSFEEVVDYASEVGFKSLEVCSWPKGKALRR
                     YAGVTHIDVDLLTKEKAAYYNNYAKNKGIEISALAYFPNPLSDDQDRAQVAREHIKKL
                     IRAAALMGVPRITTFIGKNKNLTLEENLENMKRVWKPILSLAEEHKIAIAIENCPMFF
                     TKDEWPGGNNLASTPFVWKQMFEMSPQIGLCYDPSHLVLLGMDPIKPVRDYPNRIFHV
                     HFKDLRINQEQVEEFGRFSYPSLWHSPKLPGLGDVDFPHLIATLNEVGYKGAGCLEVE
                     DRAFEGSLEDTKKGIEMSYRYLHTLMG"
     gene            10483..11454
                     /locus_tag="VFD08_05345"
     CDS             10483..11454
                     /locus_tag="VFD08_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZK10478.1"
                     /translation="MLKIGLVGLGGMGTVHYSNYAVMEGVELVAATGTSDKDREKAKK
                     WGIPLYKSLSQMLEEEELDLVDICTPTFLHKEHVMAALERSLPVICEKPLALKVEDAK
                     EMFEKAQEKGVHIYVGQVVRFFKEYQALSKVIQSGEYGKVLDAQFLRLSARPRWNQGG
                     WMFEKDKSGQLPFDLHIHDLDYIVSVFGKPERFSFSSAGNKDQDFKEHYRFNYDFGDF
                     HVSAEAAWYNADYPWTAEYRIYFENAVMESKNGRVVAYQFDKEARVFDTEEELKIPTG
                     INVPPTGVYFEELSHFIDCIKANKPSDIITKDQVIIVLETLNEIVKE"
     gene            11467..12420
                     /locus_tag="VFD08_05350"
     CDS             11467..12420
                     /locus_tag="VFD08_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZK10479.1"
                     /translation="MIRLGVDIGGTNIKIGLLKDEGRIIEKKEFPFSHDGYKSVFSQI
                     EKEIRTLLDQEGFALGQVQSIGLAIPGSIDEKKGIITHAYNLDFYDVPAVGQMQKAFP
                     DTPIFLANDANAAALAEFYSGVFKGYRTAVLLTIGTGVGGGIICDGKMFNGGQNNGVE
                     LGHMMLKYGGIQCSCGNYGCVESYCTATWIADQGKKLIKEKKDSVVTSRGKGKPENVD
                     AKLVIDAAREGDLQAMEIFETFVDNLSAAITSINALLDPEIIAIGGGVSGSGDILFES
                     LRRKVKEKSFFKVDYKVEPAHLGNDAGIVGAAMLYKNKETL"
     gene            12522..13505
                     /locus_tag="VFD08_05355"
     CDS             12522..13505
                     /locus_tag="VFD08_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK10480.1"
                     /translation="MSRKILVFFLVAMLVMTTVAGCAGGSDEPADSEEVEETQEAQGE
                     TYKVGILAPAVTHGWVAAVAYNAELEAQELGVDYQVQTSTNAEEMTAQLEDLVTWGAQ
                     AVVAFPQWEGMEIPIQEAIDEGITVVNFDIQIDADGIYLVTGDNYDMGVQGAHYILDK
                     IGPEGVVVALPVPQSGSVSELRMAGFTETAEELDQNWEILEYATAFTREDGLADFADI
                     LAANPQIDAVYSLDDETSIGVIQAITEAGRTDIKVITGGGGNQEYFNMMPEYEDMYVQ
                     SALYSPAMVREAVKMAVDILAGKDVESPMVIPTSIVDKDNYTDYLDADSPY"
     gene            13656..15173
                     /locus_tag="VFD08_05360"
     CDS             13656..15173
                     /locus_tag="VFD08_05360"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZK10481.1"
                     /translation="MKLEMLNIVKSFGANDVLKNVNFHLFGGEIVALLGENGAGKSTL
                     MNILGGVVKEDSGRILINDDLRDFAKPLDSLNAGIGFIHQELNLLDDLAVYENMFINR
                     ELKTRGGFLDIPAMCRECAEVFKRMDVEIDPKTMVRELDASYKQIVEIAKALMMEAKI
                     IIMDEPTTALTEPEIKRVFAMMKTLKEQGVAMVFISHKLKEVIEICDRYCVLRDGNLV
                     AEGQVKEVTVNQLAKYMVGHEVRTQKLQRKTEKGKEVLRGENLSFASSFKDISFAINE
                     GEVLGFTGLLGDGRSELFRTIFGCNKGYQGSIFLDDKKIRIRNTKQALELGMGYVPRN
                     RKENGIIKDLNILENGTIVIWPQLVTKFLINRKRQEKEFQSKVDGLAIKLERYTDSIN
                     SLSGGNQQKVVLAKWLSANPKVLILDNPTQGVDVGAKEDIYDIILDLAQAGMAVIVLS
                     SEAQEVIRLCDRAIVMYHGMIQAEVSAESMTEENIMRLATGGHVKEMGGRDHGKN"
     gene            15160..>16180
                     /locus_tag="VFD08_05365"
     CDS             15160..>16180
                     /locus_tag="VFD08_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10482.1"
                     /translation="MEKTKEKKITFGRWFKNQWVNNLLFSTGIILIVMLILQTQALGF
                     DYASFGEWFAAWIRNLTNVLRNNAGIGIIALGMTFVIISGGIDLAVGSTLVTVGAAIM
                     LLMDTGPNGLLGQIGISGVPAYLIAIILGALVGILLGEVTGILITKGRIPPFIVTLGT
                     MQFFRSVTQHFMQGHNPAVPKGFLPLASYKIGNLMLMPIIYWFIAAVIMHIISTRTVF
                     GRQVYAVGSNERTARLSGINVHRVKRRVYVLMGLLISFSAIIQVARIGSMDYANAGAG
                     YELDAIAAVVIGGTSFAGGRGSMLGTVLGVMIIAVMNNLLNLWGVPPFLREAFKGAIV
                     IVAVLM"
BASE COUNT         5469 a         2675 c         3678 g         4358 t
ORIGIN      
        1 aaactatacg gaaatatggc aacaacactg ggagttcatc ccgtattaat ggttaaggcc
       61 atgtttagat atcgctggat ggtttcttat ctaaccgcag caaatatgat agatagacat
      121 accattggta tcaaaggaaa agaattacga tacattcatg aagagttcta ttccattgta
      181 aacaattcaa tcaatacgat taaacaaatc atcgaaagag atgaaaactt aaatccaaat
      241 agtaaaaaag cagctaaatt aagagaaaac actgttatgt ttgatgaaat gacacctacc
      301 ttaattatgg cgggctttcc aaccctagac tggattgatg tagccatgtt tgttattggc
      361 ttacctggcg aaattgacca aattgcgaac atatattaca ttgatgccat tgaacatttt
      421 ggactggctc cagatgtatg tccacttcca gcagcagaag ttggagcagc tgtcgtggat
      481 gattacccca gagtaggtaa agcctttatt acaagttcta tgccttgtga tggttccatt
      541 gggcagacaa catttatgtc aagatacttt aaagatattc ctgtatacca aattacccca
      601 ccccaacgtt ttcttgagcc agaagtacaa aattatgcag taaagaacct agaaggttgc
      661 attgaattta ttgaagataa ctttgatgtt aaatgggatt gggatgcctt ttgggaaggt
      721 tgccaaatgt acaaccagtc atcacagtat gcaataaaca agtgggacgt aaacacgact
      781 ccttatcctc aggtaaaggg ctcagcacta gcgcttgctc gtcagtacca ataccaaggc
      841 gcgggatgcc tagatccatt tatgcttgag accaatgcaa aagttgataa gatgatgaaa
      901 aaaggctacg aagaagacaa gaaaaatgat gcaaacaagc caaaatatcg tgccattgtt
      961 tgggcttgtc cagcacacta ttatacaaac tttacctatt ggacaagaca ctgctggggc
     1021 gtagaatgct taatcgacat ggaagttatg ctttcctatc ataagatcaa aattggtgat
     1081 gaaagacaag ccatgataga tttagcaaat agctatgagc gaatgatgat gcgctcacat
     1141 acaaatggtg gttatgtaaa ctccttggat gaatgctgga agatgtgcga gaaaatggat
     1201 gtaaatatcg taattatgta cgatcatgtt tcttgcaaaa acgtaggtgg cttacacggc
     1261 ctatacgaag atcaagcaag agaacgtggc attcacctaa tttgggttcc tcatgactta
     1321 atggatccaa gaacggtttc cagaagagcc atgcgagatg cctttaataa ctatatgatt
     1381 aatgtattta atgaaaaacc attagatcct acattagtag attatcctga tgaattaaca
     1441 tggtagaaag ataaaaaagg aggatgtaaa atgaaatatt ttggtggatg tgacgttggt
     1501 tcaacatata caaaagctat aatcttagat caagacggaa atatactttc tgatgtaaca
     1561 ataaaaagca agatcaatag tgaagaaagt gcagagcttg ctatggaaaa agtaattgaa
     1621 aagacaaaag gcatagacaa gctggaagat ttaacctact taattggtac aggctatgga
     1681 agaaataagg tgccacaagc acaagaaaat atttctgaga tttcctgtca cgccatgggc
     1741 gtacatgtta caaatccaga tgtaaaagct attatagata tcggcgggca agacgtaaaa
     1801 ggaattgctg ttgatgatga tggaacagtt aagacctttg caatgaatga taaatgtgct
     1861 gctggtaccg gccgtttctt tgaggccatg gctagagctt ttgaactctc attagatgac
     1921 ttttccaagc tttcactagg ggcaaaaaac gcaattccta ttacagcaca gtgtgtagtt
     1981 tttgcagaat ctgaagtaat tactctggta ggagaaggaa aacctcgaga tgaaatcgca
     2041 gcggggattc aacagtctgt tgccaaaaga tgctttgtta tggccaaaaa agcaggagcc
     2101 aaagactcca tcacccttac gggtggatgt gctaaaaaca taggacttaa aaaagcaatt
     2161 gaaaacgtac ttcgaatcaa agtaattgat ttggatgtag atcctcagtt aatgggcgca
     2221 ctaggagcag cggaatttgc aagacaaaaa gggctggata tttacggata cggcgcataa
     2281 cataggggga tagaatgaaa aaatcaagca aaaagactct gtttaagatt ttgctgggct
     2341 ttgtagtatt actaagtggt ttatttgttg tatatattac aaacttagat ggaaaaatga
     2401 tagaacagac ctataatttt ttaatcaagt accatgacca aagagacgat atagtcgaag
     2461 cactatagtt ttaaggagga cgatctgaaa gtatacgaca agataataga taaaacgtta
     2521 aagattcttg agccgtacaa tatgaaagaa ttagctgtaa atccaaatgt aaaatggaat
     2581 tccctagaac acaatgagtt tctaatggga aatgaagtgg catttgaatt aggagatcca
     2641 ttcgagccat gcacagtata caattgttca acaagtggcg gaatatttgt accggaagat
     2701 cgcatctcct tgattggaaa agacctacct gaattaaagg gtaatactaa cttttctcga
     2761 attacctttc ttgatattga tgatgtagag gatccaaaca aggcctatac acgcataaag
     2821 cgcttggaat ttgaacgttt taaagtaatt ccagaaggat atatgatttt aagctctagt
     2881 gtagaaaata aagaacaggt tagggttagt aaaaaggcca taaaaaacgg tttggatttt
     2941 tctaccattg gaaacttgtt tatcaatagg tacaaaaagg ttgaaggagt aaacaatgta
     3001 tggatgattt tcattgtggg agattatcca tttattccag aactagtaaa gctaagtggt
     3061 gaagtagatg aaatcactaa tgcatttgaa catgtcttaa gggatattat tacagactgt
     3121 agtatttgtc ccttgcaaac aatatgcgat gatgttgagg cattaagaga acaacatttc
     3181 caagcattac aagagaaaaa agagaaaaaa ggaaaaaaaa ggaaaaaaag gaaaaaaaga
     3241 tgagtgaact gaaaaaagac ccgctagtac aaaaactagt agacagttat ggggactggt
     3301 ttatagacag tcctcaagat gcaagagcta tgacagggga attatatcct tttgaaacaa
     3361 tgttttctcc tattagcatc aatagaacaa aaattaaaaa taggatcgtc atggcaccca
     3421 tgggcaatct tcaaatgtgt gaagaaaacg gacgccctaa tcaaaagatg attcaatact
     3481 ttactgagcg agcaaaaggt ggatgcggtt tactaacaac cggacttata cctatttctc
     3541 atggaattga tccaaccgtt acagaaccag gtggattatc ttatttcccg agaattgatc
     3601 gatcaagaac gaacttagtt ggctggcgtg aactagctca aagtgtacaa gcctatggtt
     3661 caaaaatatt tatccaacta acaccaggac ttggtcgtgt gggcccacca atttgcttac
     3721 cgactcaact taagtttccc gtttcatctt catttaacaa aaacttctat attcccggtg
     3781 ttccatgctt aagactttct gattttaaat taaataaaat cataagaaat acagcacaag
     3841 cggctattga cgctaaacac caaggaatcg atggagttta cttacatgga catgaaggat
     3901 acttattaga gcaaatgaca aatcctgcat ttaaccgccg taaactaggt aaatatgcag
     3961 acccacatcg ttttggtgtg gacttagtaa aaggagttcg tgagagtgtc ggaccatttt
     4021 atccgattat gtaccgtatt gatttatctt tagcccttaa tgaaacctat gaagatattg
     4081 aaaatatgcc tacactcaaa aagtatacca atggtagaac aatagctgat acgctcaaat
     4141 ttatggaagc cctagtagaa gcaggagtag actgctttga tgttgaccta ggttgctatg
     4201 ataactggtg gttacctcat cctccaggaa gcatgccagc aggctgcttt acctctgtat
     4261 cagaagctgc agtagatcat ttcagaaaaa ataatattaa aacaaatctt ggaaatgatg
     4321 tagtagttgt agcagttggt aaactgggat atcctgactt agccgaagaa gtactaagag
     4381 acaataaggc agatatggtt atgcttggta gacaactact agctgatccc tactggcctg
     4441 aaaaatgtta caataacaaa attgatgata ttagaccctg tattggatgc caagaaggct
     4501 gtatcaacga atttgtagaa ggtggacata ttcagtgtgc cgttaatgca cgcaccggat
     4561 ttgaagacaa atatcaaata ccggcaaaag tagaaaaacc taagaaaata gcagtagttg
     4621 gagcaggacc agcagggtgt gtatttgccc ttgaatctgc taaaaaaggt cacaatgtta
     4681 ccatatttga agcagcagat caaattggtg gtaatttaat ccctggtgga acaccaatga
     4741 ttaagtttga tgttaaaaac tactcagatt acttaaaagt tcaattagca aaagccgaaa
     4801 agaacttaaa acttaagctt aaactaaata caaaagttga aattgaagac ctgaaaaaag
     4861 gcaagtttga cacagttgtt gttgctgttg gtggagcaga tatagtacct ccattcccag
     4921 gacttgagga tactcctcac gcactggcaa cagatgcact aatagactca tccttcctag
     4981 agggcaaatc aaaaattgct attataggcg ggggagttgt tgggatagag acggcttaca
     5041 aattggcttt tgaagagaac aaatgcgttg atgtaattga aatgctagac gactttatgg
     5101 ttggagtttg tacagccaat cgtggtcatc tactaaatta tatgagactc aatgatagag
     5161 taactcttca taatggatgt aaagtgttag gatttgaaaa tggaaaagtg ttaacagaac
     5221 aaaaaacatg taaacatctt ccagatccat ataattcatg gtcgccatta ctacctgaga
     5281 atgtgcccaa cccactagca aaaccaacag gtacagcagc agtgaaaaaa gaagttgcgg
     5341 ctgactatgt acttcttgct cttggatatc gcgcagaagc agatatttac tatgatgcta
     5401 ttaaagaaag agttgctcca gaaatatata atgtcggtga ctcctttaag ccagcaaaaa
     5461 taataaatgc agtaagaggc gcctacgctt tagcaatgga agtataagct ataattagct
     5521 aagagtttaa aagtaaaact actccttaga aaaagaaaaa tctggggagt agttttttct
     5581 tttatagaaa gcaagaagga agtggccagc acctcgtaga ttgttggtat tttaacaagc
     5641 aaatgggtat atatatagtt agcaagtaag actaaagact ttaatggagg tatgttaatg
     5701 aaactagttg tactaggtgg agcgggatca caagcacttt atggaattag aaacttaatt
     5761 agccagggaa gtgtttttag tgaggtagtt atttcttctc gtaatattga aaaaaacaaa
     5821 gaaatagtca aagaactaaa ctcaccactt gtaagggcag cccaagtcga cgtatctgat
     5881 aaagaggccc tatatgaact gattaaagat tgtgatgttg ttgcaaattg tacgggcccc
     5941 tatcacatcc tggcctatga tattattgaa accacaataa aagcaggcaa agattatatt
     6001 gatttttgtg atgatatcga ggctttcaat gaaattttcg aatcagagct ccctaaaaaa
     6061 gcaaaagaaa aaggcctaag tatgattatg ggacttggtg ctagccctgg gtttttatca
     6121 attttagcct gtaatgcaga gaaaagatac tttgattcac tagatgaagc aatcttttac
     6181 tttgccacag atgaaagaga acctggtggt ccagcagtaa tggcccatct attagaatgt
     6241 gtccacaatg ccccctacat agaagaagga gaattaaaaa acggaccttc ttttcgagaa
     6301 gaatgggaat ttgattttgg aagcactttt ggaaaacgta cagtaacaag aattggacac
     6361 ccagaagtct ttacctttcc tagatattcc cctgatataa aggacgtctc tgtgagattt
     6421 agcctaaaac caatttctgc ctatgaagga ttcaagaaaa tgagtttgtc tggcttgact
     6481 tcagatgaaa aactgagtat aaacggcaat cccgtatctc caagggattt tgctctagct
     6541 gttcttgctg tacaaaaagc aggaaaaaaa gaaatgacaa aagaagaact tcaggaatta
     6601 ttaaaagaag tgactgcttg tgcagctaca gaactacatg ggaaaaaaga tggaaagagc
     6661 gtctcctata taggaaggct agaaggaaat atggccccac ttacagcaat ccccttgatt
     6721 gttggtgctg aaatgttagc aaaaaaagag attgctaaaa agggcatcct ggttgttgaa
     6781 gaagcaattg aggaaaccga aaagtttata gatgaaacgg caagaagaat tcgtggagat
     6841 ggattcccct ttgttattaa agaagaattc acgactagaa aagagtattg atcctgcttt
     6901 aaaaaaggac taagtagtat tttgctataa ggagcaagaa gtatggctat ggaatttgtg
     6961 cggtgttttt actcaaagac aagttccata gcccttttat atttaaggca taaaagaaat
     7021 ctttgggaag gagggtttat gaaaaaatcc aaagaaaatc ttattggaag actaatcatt
     7081 gtaatttcta ttatcttaac cataataggt tatgtttttc ttcctaaaga cctaattgta
     7141 caagttaccc ctagcggcga ggcgagatat agtcttccta aaatcttagc tttatcaatt
     7201 ccttttgcca taagcacact cttttctatt ctttatatgc agggtgagcc aaaatttaga
     7261 aggaaaaacc ttattctttc tattctgggt attgttgttt ttatatttat cttctttgca
     7321 aattatggag gataaaaggc cttcttatgt tactcagctg agtaacattt tttattatta
     7381 gaaagaaata ataagaagga tataaccgca tgtagccagc agcaagttga aggattgtag
     7441 cttttgggtt aaaataaggg tacaaaagag gcttatttag taataactcc aacagagcat
     7501 acagtagaaa aaacaggagg tagcataatg tcaagaatga taggagtaaa taccaattgt
     7561 tatcacggct atacaataga agaagcccta gaaggaattg caaaagcagg ctttcattat
     7621 gtggagttaa cggcgacaaa gggatggact gagcatgttt ttccagacca aagctttgag
     7681 aaattagtca aaatcagaaa taaaataaaa gagttggacc tgcatccttt tgccctgagt
     7741 ggacacacta atttgatgga tcctgaaaga attcctgatt ttgttatgaa tatggagctt
     7801 ggggcctttt ttggttgcca gtatcttgtt tcttctgtgg gagaagctca tttagaggat
     7861 aaagcccatg ctacaaatga agaagtggca ggagctgtaa ggacctttct tccttattta
     7921 gaagaaagag atatgaagct ggtcttagaa gttcatggag atcatggaac aggtagaata
     7981 ttaaagaaaa tcgtagaact agtagattca ccgcgagtga tgattaacta tgatacggcc
     8041 aatgtcctat tttttagtga tgcccatatg gaagaagatc ttgacttttc cctggacaaa
     8101 atcggctttt ttcatttaaa ggacaaggca ggtgggagaa aagaatggaa cttcccagct
     8161 ctaggcaaag gctatgtaga ctttcccatg gtatttaaaa aactagaaga agcaaaaaac
     8221 aagagccctt tttccattga gattgaattt accgacaagg gtccaaagga cctggaagaa
     8281 ataaatcaag cagtaaagga ctcctacgac tatcttagat cccaagggtt ttccatggaa
     8341 aaaatatagg cagttaccca agacagctag gaagtctagt gaaaacttat gaaaaggaga
     8401 tggatccatt gaaaaagatt agagcgggaa tcattggtgc tggatttatc ggagcagcac
     8461 atatagaggc cctaagaaga cttggcaatg tcgaagtagt agccttggca aaaacaaaag
     8521 gagcaaggca aagagcaaaa gaactctttg ttgccaaggg ctataaagac tatagggaaa
     8581 tgatagacaa agaaaattta gatgctgtcc atatctgtac tcccaactat cttcactatg
     8641 aaatggccat gtatgcaatg aaaaagggcc tggcagttat gatggaaaaa cccttcacag
     8701 tgagcctaga agaagcgcaa aaactagcaa agtatgctag ggacaataat atagtagggg
     8761 gcgtcaatca tacccttcgt atgtttcctc aagtgatgca aatgaaagaa cttatgcaaa
     8821 agggagaagt aggagatgtt tttgctgttc atggctgcta tcttcaggac tggctttatt
     8881 tggacaccga ttggaactgg cgcttagaag cagatgttaa tggaaaaacg agagcctttt
     8941 cggatatagg gtcccactgg atcgatatgg ttgaaaatat tattggtcaa agggcagtgg
     9001 aattactagc ggaatttcgt attgtccata aaaaaagaaa aaaaccatta aaagagattg
     9061 aaacattttc cgcatttgca ccaagggctg aggattacga agaaaagaca attgaaacag
     9121 aagactggtg taccattttg tttaagtttg aaaagggcgc gattggctgc gtcaatgtta
     9181 gccaggttac agcaggaaga aaaaaccaac aggtaataga aataagcgga agcaaatgtt
     9241 ccctcaagtg ggatagcgaa gactccaacg aactttggat tggaagacgg gaggcataca
     9301 atcaaaaggt gcccaaggat ccctctttgg tagagccaaa atccgctgag cttatgggat
     9361 atccaggagg ccatgtggag ggcttccccg atacctttaa aatgcagttt aagaagttct
     9421 acgaggccat tgaaaaaaaa gactgcgaaa atgtcgactt cgccaccttt gaagatgggg
     9481 tcagggaaat gcttcttaat gataaggtat acgaatctgc ttctaaaaga gcctgggtca
     9541 agatatagga ggaaagcgta atgatgaaaa tgggactatt aacagcaata ctgccggact
     9601 tgagctttga agaagtggtg gattatgctt ctgaagtggg ttttaaatca cttgaagtat
     9661 gctcttggcc taagggaaag gccctacgta gatatgcagg agtaacccat attgatgtgg
     9721 accttctaac aaaggaaaag gcagcctact ataataatta tgccaagaat aagggaatag
     9781 aaatatcggc tttagcctat tttcccaatc ctctttcaga tgatcaggac agagcccagg
     9841 ttgccaggga gcatataaag aaactaataa gggcagccgc ccttatgggg gtgccaagga
     9901 ttacgacctt tattggaaaa aataaaaatc ttaccctgga agaaaaccta gaaaacatga
     9961 aaagggtatg gaagccaatt cttagcctgg cagaagaaca taagatagcc atagccatag
    10021 agaattgtcc tatgttcttt accaaggatg agtggccagg aggcaataac ttggcatcca
    10081 ccccctttgt ttggaaacag atgtttgaga tgagtccaca aattggacta tgctacgacc
    10141 cttcccatct tgtgctctta ggaatggatc ccatcaagcc tgtaagggac tatccgaata
    10201 gaatatttca tgttcacttt aaagacctaa gaatcaacca agagcaagtc gaagagtttg
    10261 gaaggttttc ctatcccagc ctctggcact caccaaaact tcctggcttg ggagatgtgg
    10321 attttcctca tctcattgcc actcttaatg aagtgggata taaaggagct ggctgtttgg
    10381 aagtcgaaga tagggccttt gaaggatctt tagaagatac taaaaaaggc atagaaatga
    10441 gttatcgata tttgcatacc ctgatgggtt aggaggaaaa aaatgcttaa aattggcctt
    10501 gtgggtctgg gaggaatggg gaccgtccat tatagtaact atgctgttat ggagggagta
    10561 gagctagtgg ctgctacagg aacttcagat aaggaccgag aaaaggcaaa aaaatggggc
    10621 attccactct ataagtccct tagtcaaatg ctggaggaag aagagcttga tctagttgat
    10681 atttgcactc ccacctttct tcataaagaa catgtaatgg cagccctaga aagaagcctg
    10741 ccagttattt gtgaaaaacc tttggcttta aaggtggaag atgcaaaaga aatgtttgaa
    10801 aaagcacagg aaaagggtgt tcacatttat gttgggcagg tagtgaggtt ttttaaagaa
    10861 tatcaagccc tgagcaaggt aatccaaagc ggagaatacg gcaaggtatt agatgctcag
    10921 tttttacggc tctcagcaag accgcgctgg aatcaagggg gctggatgtt tgaaaaagac
    10981 aagagcggac agctgccttt tgatctacac atccatgatc ttgattatat tgtaagtgtc
    11041 tttggcaagc cagaaaggtt ttcttttagc tcagcaggaa ataaggacca agactttaaa
    11101 gaacattacc gctttaatta tgactttgga gattttcatg ttagcgcaga agcggcttgg
    11161 tacaatgcgg attatccatg gacagcagaa taccgcatat attttgaaaa tgcggtaatg
    11221 gaaagcaaaa acggtagagt ggttgcctac caatttgata aagaagctcg agtttttgat
    11281 acggaagaag aactaaaaat acctacaggg attaatgtcc caccaaccgg tgtgtatttt
    11341 gaagagcttt cacattttat agactgcatc aaagcaaata aaccttcgga tataatcact
    11401 aaggaccaag taattatagt gctagaaact ctaaacgaaa tagtcaagga atagaggggt
    11461 ttttacatga ttagactagg ggtcgacatt ggtggaacca atattaaaat aggattatta
    11521 aaagatgagg gaagaattat agaaaaaaaa gaattccctt ttagtcatga tggctataag
    11581 agtgtttttt cgcagattga aaaagaaatc cgcaccttat tggaccagga gggatttgct
    11641 ctggggcaag tccaatctat tggtcttgca attcctggaa gtattgatga aaaaaaggga
    11701 atcatcactc acgcctataa tttagacttt tatgatgttc ccgctgttgg gcagatgcaa
    11761 aaagccttcc ccgatacgcc aatttttctt gccaatgacg ccaatgctgc ggccttagca
    11821 gaattttata gcggggtctt taaagggtat aggacagccg tcctactcac cattggaacc
    11881 ggcgtggggg gcggaattat ttgtgatggg aagatgttta atgggggaca aaacaatggc
    11941 gtagagctgg gacatatgat gcttaagtat ggaggaatcc aatgttcttg tggtaattat
    12001 ggctgcgttg agtcctactg caccgctacc tggattgccg accagggaaa aaaactaata
    12061 aaagaaaaaa aggatagtgt agtgacttca agagggaagg gcaaacccga aaatgtagac
    12121 gctaaactag taatagatgc tgcaagagag ggagacctgc aggctatgga aatatttgaa
    12181 acttttgtgg ataatttaag tgccgcaata acttccataa atgccctatt agatccagaa
    12241 attatcgcca ttggtggcgg tgttagtggt tcgggtgata ttctgtttga aagcttaaga
    12301 agaaaagtta aagagaaatc ttttttcaag gttgactaca aggtggagcc agcccatcta
    12361 ggcaatgatg ctggaattgt aggggctgct atgctatata aaaacaaaga gactctgtaa
    12421 aggctgtcta agaaggggga attattgcat tagcagggga atgttggtat aatgatggtg
    12481 ttagttgatc ccgcactaga cgtaaaatta ggaggaaata aatgagtaga aaaattcttg
    12541 tattttttct agtagctatg ttggttatga ccactgtggc tggatgcgca ggtggatcag
    12601 atgagcctgc agactccgaa gaagtggaag aaactcaaga ggctcaaggt gaaacataca
    12661 aagtaggaat tttggcacca gcagttactc acggttgggt tgcggcggta gcttataatg
    12721 cagaattgga agctcaggaa ttaggagtag attaccaggt tcagacttcc actaatgctg
    12781 aagagatgac tgcccagcta gaagacttgg taacttgggg agctcaagca gttgtagcct
    12841 tcccccaatg ggaaggaatg gaaattccta ttcaagaagc aattgatgag ggaattactg
    12901 ttgtaaattt tgatatacaa attgatgcag atggtatcta ccttgtaaca ggcgataact
    12961 atgatatggg agttcaagga gcccactata ttctagataa gattgggcct gaaggagtcg
    13021 ttgttgctct tcctgttccc cagtccggat ctgtctcaga acttaggatg gctgggttta
    13081 cagaaacagc agaagaatta gatcaaaact gggagattct tgaatatgca acagccttta
    13141 ccagagaaga tggactagct gactttgcag atattttagc agccaaccca cagattgatg
    13201 ctgtatattc tttagatgat gaaacttcta ttggtgttat tcaagcaatc accgaagcgg
    13261 gaagaacaga tattaaagta atcaccggtg gtggtggaaa ccaagaatat tttaacatga
    13321 tgcctgaata cgaagatatg tacgtacaat cagctcttta ctcaccagca atggttcgtg
    13381 aagcggtgaa aatggccgta gatattctag ctggcaaaga tgttgagagc ccaatggtta
    13441 ttccaacaag tatcgttgac aaagataact atacagacta tctagacgca gattctccct
    13501 actaagatta gattttatct aaagagtaaa ataagggaat ttgtattggt aagtgggatt
    13561 caacttaaga aaagataaag taattaatag ctggggctat ggctacttag ccatagcccc
    13621 ttcttcttag tagaaccagg aaagggtcga cgagtatgaa gctagagatg ctaaatatcg
    13681 taaaatcttt cggagcaaac gatgtattaa aaaatgtaaa cttccattta tttggcggag
    13741 agattgttgc tcttttaggt gaaaatggag caggaaaatc caccctgatg aatattttag
    13801 ggggagttgt taaggaggat tctgggcgta tcttgattaa tgatgatctc agggactttg
    13861 ccaaacccct agactccttg aatgctggaa taggttttat tcatcaagaa ttaaacctac
    13921 ttgatgacct agcggtctat gaaaacatgt ttataaatag agaattaaaa acaagaggtg
    13981 gctttttgga tattccagct atgtgcaggg agtgtgcaga agtttttaag agaatggacg
    14041 ttgagatcga tcctaaaacc atggtaagag agctggatgc ctcctataaa caaatcgtag
    14101 aaatagccaa agccctgatg atggaagcca aaataataat aatggatgag cccactacag
    14161 ctcttacaga acctgaaata aaaagagttt ttgccatgat gaaaacctta aaagaacaag
    14221 gggtggcaat ggtgtttatt tcccataaac tcaaggaagt aattgagatt tgcgatcgct
    14281 actgcgtttt aagagatggc aatcttgtag cagagggcca ggtaaaagag gtaacggtaa
    14341 atcagttagc caaatatatg gttggacatg aggttcgaac gcagaaactt caaagaaaaa
    14401 cggaaaaagg aaaagaagtt ttgcgaggag aaaacctgtc ctttgcaagt agtttcaagg
    14461 atatcagttt tgccattaat gaaggcgagg tccttggctt tacgggactc ttaggagatg
    14521 gtcgcagcga gctttttcgc actatttttg gatgcaataa gggttaccaa ggctctattt
    14581 ttttggatga taaaaaaatt cgcataagaa ataccaagca ggccctggag cttgggatgg
    14641 gatatgttcc acgaaacaga aaagaaaatg gtattattaa ggacttgaat attttagaaa
    14701 atggaacgat tgttatatgg cctcaactgg ttactaaatt tttaataaat cgaaaacgcc
    14761 aagaaaaaga gtttcagagc aaggtggatg gtttggctat aaagttggag agatatactg
    14821 atagcattaa tagcctatca ggagggaacc aacaaaaggt tgtgctagca aagtggctat
    14881 cagccaatcc aaaagttctt attttagata accctactca aggagtagac gttggagcca
    14941 aagaggatat ttacgatata atactagatt tagcccaagc aggaatggca gttattgttt
    15001 tgtccagcga ggcacaggaa gtgatcagat tatgtgatcg tgcaattgtt atgtaccatg
    15061 gaatgataca agcagaggtg tcagcagagt ccatgacaga agaaaacatc atgcgtctgg
    15121 ctaccggagg acatgtgaaa gaaatgggag gtagagacca tggaaaaaac taaagaaaag
    15181 aaaataacct ttggccgatg gtttaaaaac cagtgggtaa ataacctttt gtttagtaca
    15241 ggtattattc tgattgttat gttgattctt cagacccagg cattaggatt tgactatgct
    15301 tcctttggag agtggtttgc tgcttggata agaaacttaa ccaatgtact tagaaataat
    15361 gcaggaatag ggattattgc actggggatg acctttgtta ttatctcagg agggattgac
    15421 ctagccgtag gttcaaccct agtgactgtg ggagcggcca taatgctgct tatggataca
    15481 gggcccaatg gactattagg gcagattggt attagtggag tcccagccta ccttatagcc
    15541 attattttag gagccctggt tgggatactc ttaggagaag tgacaggaat acttattaca
    15601 aagggaagaa taccgccctt tatagtcacc ctaggaacca tgcagttctt tcgctctgtt
    15661 acccagcatt ttatgcaagg acacaaccct gcagttccta agggattctt gcccttggcc
    15721 agctataaga taggaaatct tatgctgatg cccattatat actggtttat tgctgcagta
    15781 attatgcata ttatttcaac acgtacagtt tttggcagac aagtttatgc tgtaggaagc
    15841 aatgaaagaa cagcaagatt atcaggaatc aatgtacacc gtgtaaaaag aagggtctat
    15901 gttttaatgg gccttttgat ttccttctca gcaattattc aggttgctag aataggctct
    15961 atggactatg caaatgcagg agcaggatat gaactagacg ctatagctgc tgttgtaata
    16021 ggaggaacca gttttgcggg cggcagaggc tccatgcttg gaacagttct aggggttatg
    16081 attatagcgg taatgaataa tctactcaat ctctggggag ttccgccctt tcttagagaa
    16141 gcctttaagg gggcgattgt tattgtagcg gtattaatgc
//
LOCUS       DASRNA010000192         4254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_132247,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000192 DASRNA010000000
VERSION     DASRNA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132247"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFD08_05370"
     CDS             <1..556
                     /locus_tag="VFD08_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK10483.1"
                     /translation="YNAALLGKKLYSEMGGKAKVHIFDSKSAGGGPTLIYYKIQELYK
                     EGKSFEEIVEIVENYRDGMLTMFVSESLNNLSKAGRLSNLKLKVAQVMNILPVMTSND
                     GYILHTANARGSKKAFKKMVDTLIEFGVEGKTLSINHSGNEKWANYVEEELIKAGVTK
                     VYQQRTVLLNTLYLDLNGVIVTIG"
     gene            complement(674..2041)
                     /locus_tag="VFD08_05375"
     CDS             complement(674..2041)
                     /locus_tag="VFD08_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK10484.1"
                     /translation="MNKIDRKDLTQGTPWKRILAFSAPLLIGNLAQQLYNTVDSMVVG
                     RYVGDNALAAVGASGPLLNFLLLFFVGISTGVNIMVSQYFGGKKKEELGHSIGTAVIL
                     TALASIFIIVISPFVIKPFLQLINTPAEIIQWAETYLRILMCGIAGMAYFNILSGILK
                     GLGDSLSSLYYLLISTVLNIVLDLVFVIVFEMGVAGVSLATVIAQGFSALLTFYKLMQ
                     YQDIFKLTKESFVLRAKYISQMLRLGLPSGATQGIFSLAMLFVQSLSNQFGPQFIAAN
                     LMVMRVDGLAMMPNFSFGIAMTTYTGQNIGANRMDRVIEGTKQGTALALISSISLTAI
                     ILIFGHELMMLFTNTSELADYALKLMYIIAPGYIFFSITQSLGGVMRGAGDTLTPMWI
                     SIFLILVVRIPLAYGLTYLTRSPAYPIGRPECINVSLLFTWVVGGILTYYFFRKGKWK
                     TKSIQ"
     gene            complement(2028..3113)
                     /locus_tag="VFD08_05380"
     CDS             complement(2028..3113)
                     /locus_tag="VFD08_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZK10485.1"
                     /translation="MNNPIMNKFIVFILLLIGLIIVVRFSFLLIGILMPFVLGFIIAT
                     LANPLKKRIEKLNIPSRPSAFLSIVLIIAVIVLFFYLLFTLAKSGFHSIEQNLSLVID
                     SFSNTSRNIYSTLQKQYPRLITSDFSVFVSNLQDGGLLSLKEFNFSGKVFSFARGLPS
                     SLIFIIFTFMSSYYFSAGYDKIMGFLRKKILYFDSSKEIINSFRISIKLGLGSWFKAQ
                     LVIMSFSFLISSIFFYFLKIPYPVLVGMGLALFDGLPLFGAGAVLWPISIYYLITGNF
                     INSFIAFLLYIVIITIRQIIEPKLIGQEIGIHPLVTLLTIYLGLKLFGVAGIIIGIII
                     LVIATSILRGKAFKDFYKSREEEYEQN"
     gene            3215..4219
                     /gene="ltaE"
                     /locus_tag="VFD08_05385"
     CDS             3215..4219
                     /gene="ltaE"
                     /locus_tag="VFD08_05385"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HZK10486.1"
                     /translation="MDFRSDTVTKPTEAMRRAMLEAAVGDDVYDDDPSVHALQDKMAK
                     LLGKEAALFVPSGTFSNQLAIMTHTQRGDEIIVLEDAHIFIHEAGGPGLLSGVNMRQA
                     ASLDGNYDLEKLGDLFRGEDIHYPKTSLLCLENAHGNGRVVSLEQMAGAYKIAKEKKA
                     AVHLDGARIFNAAVFLGVEAKEIAQYADTISVCLSKGLAAPIGSVLFGSREFIEKARK
                     YRKVMGGGMRQVGILAACGLVGLEEVLPHLEEDHQLASYLASKLEEIQGLKVDQDRLD
                     INMVFFTLNEKMDLAGALGKRGIIINPEEEGEYRFVTHIGVDKEDVDHLIMAMKEVLE
                     "
BASE COUNT         1494 a          718 c          877 g         1165 t
ORIGIN      
        1 ttacaatgct gctttattgg gaaaaaaact ttactctgaa atgggtggaa aagcaaaggt
       61 ccatattttt gatagtaaga gtgctggagg aggccccact ttaatttatt ataaaattca
      121 agaactgtat aaagagggca aaagctttga agaaattgtt gaaatcgtag agaactacag
      181 agatggaatg ttaaccatgt ttgtttctga gagtttaaat aatctatcaa aggctggacg
      241 tctttctaat cttaaactta aagtagccca ggttatgaat atacttcctg taatgacctc
      301 taatgatgga tatatccttc atacggccaa tgctagggga agcaaaaagg catttaaaaa
      361 gatggtggac accctaattg aatttggggt agaaggcaag accctatcta ttaatcattc
      421 aggaaatgaa aaatgggcca attatgtcga agaagaactg ataaaagctg gtgtaactaa
      481 ggtgtatcag caacgtacag tgcttttaaa cactctttat ttggatttaa atggtgtgat
      541 tgtcacgata ggataaaaaa gatattagct tgctttagct ataagaagtg atttgctggt
      601 tttgtatttc ctagtaaaaa aggatctgct ataaatagca gatccttttt taccttgtcc
      661 cttaggggaa tttttattga atggattttg ttttccattt gccctttcga aaaaaatagt
      721 aggtcaatat accgccaaca acccaggtaa aaagaaggga aacattaatg cattcagggc
      781 gcccaatagg ataagcagga ctgcgcgtta gataagtgag tccataggct agggggattc
      841 taactacaag aataagaaaa atagaaatcc acataggagt taaggtatcg ccggccccgc
      901 gcataacccc accaagagat tgggtgatgc taaagaaaat ataacctgga gcaataatat
      961 acataagttt taaggcatag tctgcaagtt cactggtatt ggtaaaaagc atcataagtt
     1021 catggccaaa aataagaata attgcagtaa gacttataga gctaataagg gccagggcag
     1081 ttccttgttt tgttccctca ataacacgat ccattcgatt ggcaccaata ttttggccag
     1141 tgtaggtagt catggcaatg ccaaaagaaa aattaggcat catagccagg ccatctaccc
     1201 gcataaccat cagattagca gcgataaact gggggccaaa ttgatttgat agggattgca
     1261 caaatagcat agccaaacta aaaatccctt gagttgcacc tgagggaagg cctaaacgta
     1321 gcatctgaga aatatatttt gcccgcagga caaagctttc ttttgtaagt ttaaatatat
     1381 cttggtactg cataagttta taaaatgtta aaagagctga aaatccttga gcaatgacag
     1441 tagcaagaga tacacctgcc actcccatct caaaaacaat aacaaaaacc aggtctagaa
     1501 caatatttaa tacagtagat ataagcagat aataaaggct acttagcgaa tcccctagac
     1561 cctttagtat tcctgataga atgttaaagt aggccattcc tgcaatccca cacataagaa
     1621 ttctaagata agtctcagcc cattgaataa tttctgctgg ggtatttata agttgtaaaa
     1681 acggtttaat aacaaagggg gagataacaa tgataaaaat actggccaga gcagtgagaa
     1741 taactgctgt tccgatggaa tgccctagtt cttctttttt cttgccaccg aaatattgac
     1801 tcaccattat gttaacacca gtacttattc caacaaaaaa aaggaggaga aaatttaaaa
     1861 gaggtcccga tgcgcctacc gcagcaagag cattgtctcc tacatatctt cccaccacca
     1921 tggagtccac agtattatat agttgttgcg caagattgcc tattagaaga ggggctgaaa
     1981 aggcaagtat tcttttccaa ggggtccctt gcgtcaggtc ctttcgatca attttgttca
     2041 tattcctcct cacgagattt ataaaagtct ttaaaagctt ttcctctaag gatgcttgtt
     2101 gctataacta agataataat tccgatgatt atgcccgcca ccccaaagag ttttaaacct
     2161 aaataaatag tcaataaagt aacaagtgga tggattccaa tttcctgtcc aataagcttg
     2221 ggttcaataa tttgtcggat agtaataatt acaatataaa gcaaaaatgc tataaaagaa
     2281 tttataaaat tacccgttat caaatagtag atactaatag gccaaagcac tgcaccggca
     2341 ccaaaaagag gcaggccatc gaaaagggcc aggcccatcc caactaaaac gggatagggt
     2401 atctttaaga agtaaaaaaa gatagaggag ataagaaaag aaaaactcat aattactaat
     2461 tgggccttaa accaggaacc cagccccagc ttaatagaga ttctaaaact atttataatt
     2521 tctttagacg aatcgaaata caagatcttc ttacgaagaa aacccataat tttatcataa
     2581 ccagcagaaa agtaataaga actcataaag gtgaaaataa taaaaatcaa actagaaggt
     2641 aaaccccttg caaaggaaaa aacctttcca ctaaagttga attcttttag agataggagc
     2701 cctccatctt ggagattgct aacaaagaca ctaaaatcac tagtgataag tcttggatat
     2761 tgtttttgta aagtagagta aatatttcta cttgtatttg aaaaggaatc aatcactagg
     2821 gagaggtttt gttcaataga gtgaaagccg ctcttagcta gggtaaatag aagataaaag
     2881 aaaaggacaa tcacagctat tataaggaca atagataaaa aggccgaggg tctactgggt
     2941 atattgagtt tttcaattct ttttttgagg ggattggcta gtgtcgcaat aataaacccc
     3001 aaaacaaagg gcataagaat accaataaga agaaaagaaa aacgtacaac aataataagg
     3061 ccgattaaaa gcaggataaa aacaataaat ttattcatta tgggattatt catttgtgcc
     3121 tccaaagaaa attatatcat gtatcctata gaaatgcatc tacccaaatt aggttgaata
     3181 tggtaccatt acaggtagtt taggaggtaa taatatggat tttagaagtg atacagtaac
     3241 caaacctaca gaggccatgc gccgtgccat gcttgaagcg gcggttggtg atgatgttta
     3301 tgatgatgac cccagtgtac atgccctcca agataaaatg gcgaaacttc ttggtaaaga
     3361 ggcagctctt tttgttccat caggaacttt ttctaatcaa ttagcgatta tgacccatac
     3421 acaaagaggt gatgagatca tcgttcttga ggatgctcat atttttattc atgaagcagg
     3481 aggaccagga cttttaagtg gagtcaatat gcgtcaagct gctagtttag atggaaacta
     3541 tgatcttgaa aagctaggtg atttatttag gggagaagat attcattatc caaaaaccag
     3601 ccttttatgc ttggaaaatg ctcatggtaa tggacgagtt gtttctctag agcaaatggc
     3661 aggagcctat aaaatcgcca aagaaaaaaa agcagccgtt catttagatg gggcaagaat
     3721 ctttaatgct gctgtttttt taggggtaga agccaaagaa attgcccaat atgctgatac
     3781 gatcagtgtc tgtctatcaa aagggctagc cgctcccatt ggatcagttc tttttggcag
     3841 tagagaattt attgaaaaag ctcgcaagta ccgtaaagtt atgggtggag gtatgcgcca
     3901 ggtaggtatt cttgcagcct gtggacttgt tggcttagag gaagtattac cgcatttaga
     3961 agaagaccat cagctagcta gttatctggc ttcaaagctt gaggagattc agggactaaa
     4021 agttgatcag gataggcttg atattaatat ggtgtttttt acgctaaatg aaaaaatgga
     4081 ccttgcaggc gccttgggaa aaagaggaat tattatcaat cctgaagaag agggagaata
     4141 ccgctttgtc actcatatag gagttgataa agaagatgtg gaccatttaa ttatggccat
     4201 gaaagaagtc ttagagtgat cttaaaagct cagggtaaag cagaagtgac gtta
//
LOCUS       DASRNA010000193         4943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_872231,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000193 DASRNA010000000
VERSION     DASRNA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872231"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFD08_05390"
     CDS             complement(<1..195)
                     /locus_tag="VFD08_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HZK10487.1"
                     /translation="MDIQWYPGHMKKTRDLISQNYKLVDLIIELLDARAPRSSLNPIF
                     DDVLGDKKRLFLLNKSDLSDP"
     gene            complement(235..585)
                     /gene="rplS"
                     /locus_tag="VFD08_05395"
     CDS             complement(235..585)
                     /gene="rplS"
                     /locus_tag="VFD08_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZK10488.1"
                     /translation="MNYNIIRDLEREAIKKDLPSFNVGDTVRVSIRITEGKRDRLQVF
                     GGLVIKKQGGGVSERFTVRKLSGNVGVEKTFPMHSPKVEEIEVVRHGKARRARLFYIR
                     DRVGKSAKVREKIR"
     gene            complement(575..1312)
                     /gene="trmD"
                     /locus_tag="VFD08_05400"
     CDS             complement(575..1312)
                     /gene="trmD"
                     /locus_tag="VFD08_05400"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZK10489.1"
                     /translation="MKFTIITLFPDIFDSYFSMGILKRAQEEEHLEVEIVNLRDFSLD
                     KHKKADDYPFGQGPGMVLMAQPAIDALENRSHPIIHFSPRGNLLNQEKVRQLAQYEEI
                     TLLCSHYEGIDQRVLDHFVDEEISIGDYILSGGEMAAMILMDAVIRLIPGVISDESVH
                     EESHEDGLLEYNQYTRPRSFRGLDVPEVLFSGHHEKIRLYRLEEAVRLTLNRRPDMIE
                     KGIKKGIYNQEILKLIEKLREEERNEL"
     gene            complement(1309..1779)
                     /gene="rimM"
                     /locus_tag="VFD08_05405"
     CDS             complement(1309..1779)
                     /gene="rimM"
                     /locus_tag="VFD08_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZK10490.1"
                     /translation="MIEIGRTIKAVGLRGEIKIHVQQPELLEIGKNYYLEDKVYTLEK
                     IRFTKKATVIKFLGVDSIEEAQGQLQKKLSIKKEDIELEEGEFFESDLLGLKVIDEQG
                     VDLGEIVDILYPSSQTLYVIRGETEFMLPAVKEFILSIDLDKKEMTVSLIEGLR"
     gene            complement(1779..2006)
                     /locus_tag="VFD08_05410"
     CDS             complement(1779..2006)
                     /locus_tag="VFD08_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZK10491.1"
                     /translation="MSELVTSVVLRIVQYPEMVQITQTEKDGVIRVEIRVAPDDMGRV
                     IGKGGRVINSIRTIAKAGASKDNNKVYVEVI"
     gene            complement(2016..2267)
                     /gene="rpsP"
                     /locus_tag="VFD08_05415"
     CDS             complement(2016..2267)
                     /gene="rpsP"
                     /locus_tag="VFD08_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZK10492.1"
                     /translation="MAVKIRLRRMGSKKRPTYRVVVADSRSPRDGRVIEEIGHYDPLS
                     NPVKLKIDHEKAIKWLNDGAQPTDRAKTLLKKSGVFEEA"
     gene            complement(2351..3700)
                     /gene="ffh"
                     /locus_tag="VFD08_05420"
     CDS             complement(2351..3700)
                     /gene="ffh"
                     /locus_tag="VFD08_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZK10493.1"
                     /translation="MFGGLSEKLQNALNKLSGKGKVSEKDIKSVMREVRLSLLEADVN
                     FRVVKEFVSKIQERALGVEVMDSLTPGTQVIKIVRDELTQLMGSTNSKLTFNTNGPSV
                     YMVIGLQGSGKTTSGAKLAAKLKAKGKRPLLVALDIYRPAAIDQLHVLGKQLDVPVFS
                     MGDQKSPVDIAKAALDEAYNTSRDVLILDTAGRLHLDEELMQELEEIKSAMRPTEILL
                     VVDAMVGQDAVTVAGSFHEKLGIDGIIMTKLDGDTRGGSALSMKEVTGAPIKFIGTGE
                     KITGNTLEEFHPDRMASRILGMGDVLSLIERAEKSYDEQKAQALEQKMRQEGLDFDDF
                     LEQLQQMREMGGIGELMAMLPGLGGNMKALKNMDMDDKEIKKVEAIIQSMTAKERRSP
                     DILNAKRKQRIAKGSGTSIQDVNKLVKQFTQTRKMMKQFTNMSKKGKNPFGGLNLPF"
     gene            complement(3712..4038)
                     /locus_tag="VFD08_05425"
     CDS             complement(3712..4038)
                     /locus_tag="VFD08_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZK10494.1"
                     /translation="MERIREIYYMDLYGSLLTKHQQELLQMVIMEDYSLGEIAERLAI
                     TRQGVHDGVQRGLAKLQWYEDHLGLYKKHFVQKQKISKIKEILESDRENLSAEVFDLL
                     SELLDV"
     gene            complement(4208..4867)
                     /locus_tag="VFD08_05430"
     CDS             complement(4208..4867)
                     /locus_tag="VFD08_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZK10495.1"
                     /translation="MLINYVGHSTFVIKTKEKKIIIDPFILGNPWTETTLEDYSDITH
                     IFVTHGHADHLGDSVELAKLSGALVYCNHEIAAYLTSQGVENIHSMHIGGRHDWVKMT
                     PALHGSGIETPKGTVCGGLAGGFLLTIEDKKIYHSGDTGLSQEMKLLEGQVDLAMIPI
                     GGNYTMDIEDAKTALSFIKPRAFIPMHYKTFPVIEAEPKDLVMEEIQGVYLKPGESIK
                     W"
BASE COUNT         1395 a         1070 c          769 g         1709 t
ORIGIN      
        1 gggatcagat aaatctgatt tatttagtag aaataaacgt tttttgtctc caagaacgtc
       61 atcaaaaata gggttcaatg aagatctagg tgctctggca tccagaagtt caatgattaa
      121 gtcgacaagt ttgtaatttt gagaaattaa atcccgggtt tttttcatgt gaccgggata
      181 ccactggatg tccatagtac ctccttctat gaaaaagaac tgcaggagca gttcttaacg
      241 aattttttct ctaaccttgg cagatttacc cacacgatca cggatataaa ataaacgtgc
      301 acgacgagct tttccgtggc gtacaacttc gatctcttca actttaggtg aatgcatagg
      361 gaaagttttt tcaactccta catttcctga aagttttcgt accgtaaaac gttcagagac
      421 tccaccgcct tgctttttaa taacaaggcc tccaaagacc tgaagacgat cgcgctttcc
      481 ttcagtaatg cgaatagaaa ctctaacagt atctcccaca ttaaaggaag gtagatcctt
      541 ctttatggcc tctctttcga gatcacgaat gatattataa ttcattcctt tcctcctctc
      601 gtaatttttc aattaatttt aaaatttctt gattgtaaat tcctttttta attccttttt
      661 caatcatatc tggacgccta tttagtgtta gccttactgc ttcttctaag cgataaagtc
      721 taattttttc gtgatgtccg ctaaaaagca cttccggtac atcaagtcca cgaaaacttc
      781 taggccttgt atactgatta tattcaagaa gaccatcttc atgggattct tcatgaactg
      841 actcatcaga aataacacca ggaattaagc gaatcacagc atccattaag atcatagccg
      901 ccatttcacc tccggaaaga atatagtctc caattgaaat ctcctcatca acaaagtgat
      961 caagtaccct ttgatctatg ccttcataat gactgcataa aagagtaatc tcttcgtact
     1021 gggcaagttg ccttactttt tcttgattta gtagatttcc cctaggacta aaatgaatta
     1081 tcggatgtga acgattttcg agggcatcga tcgcaggttg agccattaag accattccag
     1141 gaccttgtcc aaagggatag tcgtcggctt ttttgtgttt atcgagacta aaatctctta
     1201 aatttacgat ttctacctct aaatgttctt cttcctgtgc tctttttaaa attcccatgg
     1261 aaaaatagga atcaaaaata tctggaaata aggtaataat ggtaaacttc atcttaaacc
     1321 ttcaataaga gatactgtca tttctttttt atccaaatca atagatagaa taaattcttt
     1381 tactgctgga agcataaatt ccgtttctcc gcgaataaca taaagagttt ggctagaagg
     1441 atataatata tcaacaattt ctcctagatc aacaccctgc tcatcaatta cttttaaccc
     1501 caaaagatct gactcaaaaa attccccctc ttcaagttct atatcttctt tttttatgct
     1561 gagttttttt tgcaactgac cttgagcttc ttctatgctg tcaacaccta aaaatttaat
     1621 aacagttgcc tttttagtaa aacgtatttt ctctaaagta taaactttat cttctaaata
     1681 ataattcttc ccgatttcaa gtaattcagg ttgctggaca tggattttta tctcacctct
     1741 tagtccaact gctttaatcg ttcttccaat ttcaatcatt aaattacttc aacataaacc
     1801 ttgttgttat ctttggatgc accagcttta gcaatggttc taatagagtt tattactcta
     1861 ccacctttgc caattactcg acccatatca tctggtgcaa cacgaatctc aactctaatg
     1921 actccgtctt tttctgtttg agtaatttga accatttccg gatactgaac aatacgtagt
     1981 actactgatg taacgagttc agacattgcc acctcctatg cttcttcaaa aacgccgctt
     2041 tttttaagca aagtctttgc tcgatctgtt ggctgggcgc cgtcgtttag ccatttaatc
     2101 gccttttcat gatcaatttt taatttaact ggattactta atggatcata atgtccaatt
     2161 tcctcaatca ctcgaccatc tctaggactt cttgaatcag ctacaacaac acgataagtg
     2221 ggtcgttttt tacttcccat tcttctcaat ctgattttga ctgccatata tacctccttt
     2281 atctatgtgg cctgccataa ggcttcttcg actaatctaa catgccgtcg tcccgggccc
     2341 cgggatttta ttaaaaaggt aagtttaatc ctccaaacgg attttttccc tttttgctca
     2401 tatttgtaaa ctgtttcatc atttttctag tctgagtaaa ctgtttaact aacttgttga
     2461 catcttgaat gctggtgcca cttcctttag caatgcgctg ctttcttttg gcattaagaa
     2521 tatctgggct tcttctttcc tttgctgtca tggattgaat aatagcttct actttcttta
     2581 tttctttatc gtccatatcc atgtttttaa gcgccttcat atttccacca aggccaggaa
     2641 gcatggccat aagttctcca ataccgccca tttctcgcat ttgttgaagc tgttctaaaa
     2701 aatcatcaaa gtctaggcct tcttggcgca ttttttgttc taaggcttga gctttttgct
     2761 catcataact tttttctgcg cgttctataa gacttaatac atcacccatt ccaagaattc
     2821 tagaagccat acggtctgga tgaaattctt ctaaggtatt tcccgtgatt ttttcacctg
     2881 taccaataaa ctttatggga gctccggtga cttctttcat acttagagct gaaccacctc
     2941 gggtatctcc gtctagttta gtcataataa taccatctat gccaagtttt tcatgaaagc
     3001 ttcccgctac cgtaactgcg tcttggccta ccatggcatc gaccacaagg agaatttctg
     3061 tgggcctcat tgcactttta atttcttcta attcctgcat aagttcttca tctaaatgaa
     3121 gccttccagc agtatctaaa attaaaacat ctcggcttgt attataagct tcatcaagag
     3181 ccgcttttgc tatatctact ggggattttt gatctcccat ggaaaagaca ggtacatcaa
     3241 gttgttttcc taaaacgtga agctgatcaa tagccgctgg gcgatatata tcaagggcaa
     3301 ccaaaagggg tctttttccc tttgccttaa gttttgctgc tagttttgct ccactagtag
     3361 ttttacctga accttggagt ccgataacca tatagacgct aggcccattt gtattaaaag
     3421 taagtttact attggtactg cccataagtt gggtcagttc atcgcgaaca attttaatca
     3481 cttgtgtacc tggtgttaga ctatccataa cttcaacacc cagggctcgt tcttgaattt
     3541 tacttacaaa ctctttaact acgcgaaagt ttacatcagc ttctaaaaga gatagtcgaa
     3601 cctctcgcat aacactttta atatcttttt cactaacttt acccttacca cttaatttat
     3661 ttagggcatt ttgaagtttt tcacttagtc caccaaacat ggtacctcct tctatacatc
     3721 aagcagttct gaaagaagat caaatacttc ggcggaaaga ttttctctat cactttctag
     3781 gatctctttt atttttgaaa ttttttgctt ttgcacaaaa tgtttcttat aaagtcccag
     3841 gtggtcttca taccactgga gtttagccaa tcctctttgt acgccatcat ggacgccttg
     3901 gcgagttatg gccaatcttt ctgcaatttc tccaagagaa taatcttcca taataaccat
     3961 ttgaagaagc tcttgttgat gcttagtcaa gagacttcca tataaatcca tataatatat
     4021 ttcacgtatt ctttccataa aacacccctg taaaggcttt tccctttaca ggggtatatt
     4081 acatgagtaa attgcttttg tcaagataaa aattttaggg aattcttccg tattttccaa
     4141 tatttttaaa gaaaaaggaa agctgcttca ttagtagatg attatagcag gatatgcata
     4201 ggaaaaacta ccacttaata ctttctcctg gcttaagata aacaccctga atctcttcca
     4261 taactagatc tttaggttct gcctcaatca caggaaaggt tttataatgc ataggtataa
     4321 atgctctggg tttaataaaa ctaagagccg tctttgcatc ttcaatatcc atagtataat
     4381 ttccaccgat tggtatcatg gccagatcta cttggccttc taaaagtttc atctcttggc
     4441 taaggcccgt atctccacta tggtagattt tcttatcttc tatagtaaga agaaaacctc
     4501 cagctaaacc accgcaaact gtacccttgg gtgtttcgat accactacca tgaagtgcag
     4561 gtgtcatttt tacccaatca tgacgcccac caatatgcat actgtggata ttctcaactc
     4621 cttggctagt taagtaagct gcaatctcat gattgcaata aacaagagcc ccgcttagct
     4681 tagccaattc aacgctatct cctaggtggt ctgcatgccc atgagtaaca aagatatgag
     4741 taatatctga ataatcctca agagtcgttt cagtccaagg atttccaaga ataaaaggat
     4801 cgattattat ctttttttcc tttgttttta taacaaaggt gctgtgccca acatagttaa
     4861 taagcattat aactcctttt tattttttaa attttgaaaa gaacccattt gtttttttca
     4921 atttcatatt ttaactgata gac
//
LOCUS       DASRNA010000194         5275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_208562,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000194 DASRNA010000000
VERSION     DASRNA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208562"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..627)
                     /locus_tag="VFD08_05435"
     CDS             complement(4..627)
                     /locus_tag="VFD08_05435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK10496.1"
                     /translation="MEEKKSSLLFNLIAPIYALFYHSQKRGFIKIIEKVKNEINLASF
                     ETIIDVGCGTGALCSVLNDRGMKVTGVDPAIKMLDIGRRKSDNKGIVFLQGNALEGLE
                     FEDKSFDLAISSYVAHGLEAGDRKRLYAEMSRVAKDYVIIHDYNNKRSLLTSFIEWLE
                     GGDYFHFIKHAEREMKDCLTEMTTCFSEVRVIQVGVRANWYICKPMQ"
     gene            complement(1054..2499)
                     /locus_tag="VFD08_05440"
     CDS             complement(1054..2499)
                     /locus_tag="VFD08_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HZK10497.1"
                     /translation="MKNTGWNFDNSYEKLPSTFYKKVELSPLVSPTLLVLNEKLAKEL
                     GLDPDLLKKAEGLSVLSGTKLPPGGSSISQAYMGHQFGHLSMLGDGRALLIGEQITPR
                     GERFDLQYKGSGPTPFSRSGDGRATLGPMLREYLISEAMHNLGIPSSRSLSVSLTGDR
                     VLRNSLEKGAILVRVASSHIRIGTFVYAASQKDISKLKSLADYSIERHFPELKNSQQI
                     YLDFYKKVIQGQGELIAKWMLVGFVHGVMNSDNMAISGETIDYGPCAFMDLYKKNTLF
                     SSIDRLGRYAYGNQPKIGMWNLAKFGESLIPLIDKDPKLAVKKLSKALDEYPKIYEKA
                     YYSGLGKKVGLADFDLKNKKILNELLEFMEENKLDYTNTFLDLTFKKFDKAVYASKEF
                     LDWKNKWESLLKEEGKSQQQVGEMMKNHNPAIIPRNHWVERAIKEASMGEYDLLFDFL
                     RALENPYAHKSKQEKYSVVPENPHYVTYCGT"
     gene            complement(2734..4185)
                     /locus_tag="VFD08_05445"
     CDS             complement(2734..4185)
                     /locus_tag="VFD08_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HZK10498.1"
                     /translation="MSNKKKLTIIQMNDTHGYLEEHYEHLWLGGEEKFVKAGGYARIY
                     SYVDSVRKETNNNVLFLDGGDTFHGTYPVVDSKGEILVELLNDLEVDAMTAHWEFAYG
                     PEQFNSLVSKLDFPMLAINCYKKEENKLMYPPYLVKEINGLKIGVIGIAATIIDKVMP
                     AHFSRGLYFTMGNQELPKYIEELKNKKEVDLIVVLSHLGYPQELKIAEEVDGIDVLLS
                     AHTHNRIYEPAIINNTIIIQSGSHGSFLGRLDLEVENKKIVSHKHELKLLDKSVKKDE
                     KMQAKIDRLLAPHRNMLKEVIGKTKTKLVRNRVLESTMDNFLLASLMMEVPDALMAFS
                     NGWRYGAPIPKGEIRVNDIWNIIPVNPPLSTVELTGRELWDMLEENLERTFARDPYEQ
                     MGGYVKRCMGINVYFKIENPAGERIEELFVDGKAVDKDEIYKAVYVSSQGVPAKYGTN
                     RKNLDIHAIDVLKKYLKVKGEIEAPITGTIVAI"
     gene            complement(4272..4823)
                     /locus_tag="VFD08_05450"
     CDS             complement(4272..4823)
                     /locus_tag="VFD08_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZK10499.1"
                     /translation="MTKNKKEDRLLQGLFIGILFGFFLQKGGVSKYDVIMGQLRLTDF
                     TVAKIILTAIVITTLGISVFYPLGKIQIKTKSGSIKNALIGGLIFGLGFGILGYCPGT
                     IAAAVGNGYVDALTGGFIGIVLGTILFALIYSKLKEKKILTEDRFSGKSLFHSFKGHP
                     LKYTLPISIVLILILFLLEARGL"
     gene            complement(4836..>5275)
                     /locus_tag="VFD08_05455"
     CDS             complement(4836..>5275)
                     /locus_tag="VFD08_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZK10500.1"
                     /translation="LLLSNKPLGASTSFLKLGGMFYKLFDKEKIEKNEYYKEKPPRVD
                     WGVMLVIGIVLGSFISSWLSRDFQILAVPAMWSREISSSFPLRFLFSMLGGVFLGIGA
                     RWAGGCTSGHGISGTSMLSIISWVATIAFFIGGILSSFIIFGL"
BASE COUNT         1548 a         1129 c          822 g         1776 t
ORIGIN      
        1 ctattactgc attggtttac aaatatacca attggcccta acacctacct gaataaccct
       61 tacctctgaa aaacaagtcg tcatttctgt gagacaatct ttcatttccc tttctgcatg
      121 tttgataaaa tgaaaatagt ccccaccttc caaccattcg ataaaagagg tgagtaaaga
      181 tcttttatta ttgtaatcat gaatgattac gtagtccttt gcaaccctgc tcatctcagc
      241 atagagccgt tttctatccc cggcttctaa gccatgtgcc acataggatg atatggcaag
      301 atcaaaactt ttatcttcaa attccaatcc ttcaagggca tttccttgaa gaaatactat
      361 acctttattg tctgatttcc tcctgccaat gtccagcatt tttatagcag gatcgacccc
      421 tgtcaccttc atgcccctgt catttaaaac agagcatagg gctcctgtgc cgcatcccac
      481 atcaataatt gtctcaaatg aagctaggtt tatttcattc ttaacttttt caataatctt
      541 gataaagcct cttttctgag agtgataaaa caaggcataa atgggtgcaa tcaaattaaa
      601 gagtaaacta cttttctttt cttccatagg taaaacccct ttgtttgaat ttttggtttt
      661 ttcatcttct aattcatttt aagctaatct atcaagctct ttttagcctt atcagatatc
      721 ttttttccaa agaaataggc tattagtact tacacctgct ttgttacttg caaagggtct
      781 tcttaataaa caggtgcctt aaagcttaca agtatagcta atttcttttc gggtaactca
      841 gctgcatagc acagccactc aaaaatgtta tgtttaaatt gccctctctt tttaaagaaa
      901 gcttgctctt acttttatat cccatggaaa tgctaaattt tttcttgttc tcttttagta
      961 tacagtagta agaagcatac ctttaacgaa atacccctgt taattcttac caaaacccta
     1021 caaagactat tacgctacta attgtagggc agtttatgtt ccacaatagg ttacatagtg
     1081 tgggttttct ggaaccacac tatatttttc ttgcttactc ttatgggcat agggattttc
     1141 taaggctctt aaaaaatcaa atagtagatc atattctccc atactggcct ccttaatggc
     1201 cctttctacc caatggtttc taggaataat tgctggatta tgatttttca tcatctcccc
     1261 tacttgctgc tggcttttgc cttcttcttt taagagactt tcccatttgt ttttccagtc
     1321 taaaaactcc ttggaagcat atactgcttt atcaaatttt ttgaaggtta aatctaaaaa
     1381 agtgttggta taatctaatt tattttcttc cataaattct aaaagttcat ttaagatctt
     1441 tttatttttt aaatcaaaat cagccagacc aacttttttc cccagtcccg agtaataggc
     1501 cttttcgtat atttttggat actcatctag ggctttactg agctttttta ctgctagctt
     1561 gggatccttg tcaataagag gaataaggga ttcaccgaat ttagctaaat tccacatgcc
     1621 aattttaggc tggttaccat aagcgtatct acccaatcta tcaatagaac taaagagcgt
     1681 gtttttctta tatagatcca taaaagcaca aggaccatag tcaattgttt caccagagat
     1741 ggccatatta tctgagttca ttactccatg cacaaagcct accagcatcc atttagcaat
     1801 aagttctccc tggccttgga tcaccttttt ataaaaatca aggtagattt gctgggagtt
     1861 ttttagttcc ggaaagtgcc gctcgatgct atagtcagca agagatttca gtttgctaat
     1921 gtctttttga ctggccgcat aaacaaaggt accaattcgg atatgactag atgctacccg
     1981 gaccaagatg gctccttttt ccaaggaatt tcgtagaacc ctatcaccag ttaggcttac
     2041 agacaaagag cgggaagaag gtatgcctag attgtgcatg gcctcgctta taagatactc
     2101 tcttagcata ggacctaaag tagccctacc gtcccctgat ctagaaaaag gggtaggacc
     2161 cgaaccttta tattggaggt caaatctttc tccccttggg gtaatttgtt cccctataag
     2221 aagggccctg ccatctccta gcatacttag atgaccaaat tgatggccca tataggcctg
     2281 ggagatggaa gatccaccgg gagggagctt agtgccagat aaaacagata agccctctgc
     2341 ctttttgagt aagtctgggt ccagtcccag ctccttggcc aacttttcat ttagcacaag
     2401 caaggtaggc gaaacaaggg gacttagttc tactttttta tagaaagtag aaggaagttt
     2461 ttcatagcta ttatcaaaat tccaacctgt gtttttcata ttatctcctc aactattttt
     2521 acccaagttt aaagactata ataagatgtc tttaaatagg cttagctttt tgttcaaatt
     2581 gtatctagta gcatcttcta atctttatgg ctcagaggaa cctaaagctc tggcctagaa
     2641 gtaaaagaag cccttggttt acaaaggctt ctgctcttac tagagagggg atctagaatt
     2701 agctgctagt tattataaat tttgctttta aaactaaatt gccacaatag ttccagtaat
     2761 gggagcttca atttcacctt ttactttcaa gtactttttt agaacatcaa tagcatgtat
     2821 atctaagttc ttcctatttg tcccatattt agcaggaacc ccttgggagc ttacatatac
     2881 agccttatag atttcatcct tatcaactgc tttaccatct acaaatagtt cctctattct
     2941 ctccccagct ggattttcaa ttttaaaata gacatttatg cccatacatc gtttcacata
     3001 tcctcccatt tgttcatatg ggtccctagc aaaggttctt tctaggtttt cttctaacat
     3061 atcccacaat tctctgccag taagctctac ggtagaaagg ggtggattga ctggtataat
     3121 attccaaata tcatttaccc ttatctcccc ctttggaatt ggagccccgt acctccaccc
     3181 attagaaaag gccattagag catctgggac ctccatcatt aatgatgcaa gaagaaaatt
     3241 atccatggta gattccagca ccctatttct taccagcttt gtttttgttt tacctattac
     3301 ttcctttagc atatttctat ggggagcaag taaacgatct atcttggctt gcatcttttc
     3361 atcttttttt acacttttat ctaataattt gagctcatgc ttatggctca ctattttttt
     3421 gttttcaact tccaaatcaa gtcttcctaa gaaagaccca tgagagcctg attgaatgat
     3481 aattgtatta ttaataatcg caggctcata tatcctatta tgggtgtggg cgcttaaaag
     3541 cacatctata ccatcaactt cttctgctat ttttaattcc tggggataac ctaaatgtga
     3601 taaaacaaca attaagtcta cctccttttt attctttaac tcctctatat acttaggtaa
     3661 ctcctgattg cccatagtaa agtataagcc cctactaaaa tgcgctggca taaccttatc
     3721 aataattgta gctgctattc ctattacccc aatttttaag ccattaattt cttttactaa
     3781 atagggtggg tacattagtt tgttttcttc ttttttatag cagttaatag ctagcatggg
     3841 aaagtccaac ttgcttacca atgaattaaa ttgttctggt ccataggcga attcccagtg
     3901 tgctgtcatt gcatcaactt ctaagtcatt tagtaactct actaaaattt ctccttttga
     3961 gtcaacaaca ggatatgtcc cgtgaaaggt atctcctcca tctaaaaata aaacattgtt
     4021 attagtttct ttccttacgc tgtctacata actgtagatt cgggcataac cgcctgcttt
     4081 tacaaatttt tcctccccac ccagccataa atgttcataa tgctcttcta aatatccatg
     4141 agtgtcgttc atttgaatta tggttagttt tttcttattt gacatttttg cccctttcta
     4201 cctatctagg tatagatact aaagcattta aataaaatca agaaactgac tttatttatc
     4261 tgcttgatta tttatagacc ccttgcttct aataagaaga gaataagaat aagcacgata
     4321 gaaataggaa gcgtatactt taatggatgc cctttaaaac tatgaaaaag gcttttcccg
     4381 ctaaatctat cctcagttaa tattttcttt tcttttagct tactgtagat tagagcaaat
     4441 agtattgttc ctaaaacaat cccaataaat ccacctgtta gggcatccac atagccattt
     4501 cccacagctg ccgcaatcgt acctgggcaa taacccaaaa taccaaagcc taagccaaat
     4561 attaaaccac ctatcagggc atttttaata gaaccagatt ttgtctttat ctgaattttt
     4621 cccaggggat aaaagacact aatacctaag gttgtaatta caatagccgt aagaataatc
     4681 tttgctacgg taaaatcagt caaccttaat tgccccataa taacatcgta cttgctcacc
     4741 cctccctttt ggaggaaaaa accaaataat attccaataa acaaaccttg gagaagtcga
     4801 tcctcttttt tgttttttgt catctcaaac cccttctaaa gcccaaatat aataaatgat
     4861 gaaaggatgc caccaataaa aaaggcaatg gttgctaccc agctaataat agataacata
     4921 cttgtaccac ttatcccatg gccgctggta catcctcctg cccatctggc accaatgcca
     4981 agaaaaactc ctcctagcat tgaaaacaag aatcgtagtg ggaaactaga ggatatttcc
     5041 ctactccaca tggctggcac agcaaggatt tgaaaatccc tagaaagcca ggaggatata
     5101 aaggagccta ggacaattcc tattactagc atgacacccc aatctaccct gggtggtttt
     5161 tctttataat attcattttt ttctattttt tctttatcaa ataatttata aaacattccg
     5221 cctaatttta aaaaactagt ggaggcacca agtggtttat tggatagtaa gaggg
//
LOCUS       DASRNA010000195         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_732715,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000195 DASRNA010000000
VERSION     DASRNA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732715"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VFD08_05460"
     CDS             complement(<1..1363)
                     /locus_tag="VFD08_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK10501.1"
                     /translation="MFLKSIELKGFKSFADRTHIDFLPGVTCIVGPNGCGKSNIIDAI
                     RWVLGEQSTTSLRARRMEDVIFSGTKHRAPQNFASVRLIFSGAKELSPGALDELNIER
                     ILHRSGESTYKVNGLPVRLKDLRQLFMDTAIGISGYSLISQGDIDDILKENKDERRKI
                     FEEASGISLFHYKKQEAKRRLARVEDHYLRMMDILGELDERRQPLLEEKDRALRYLEL
                     SKKAKELELFYAKEDYLGYEKEEEELGRLLGHSKYDLEKLLEKRTSMQEEKEEMEEKR
                     EGFEKEFDLLQDLLQDKIKLRDQNYLDLRIKEERQRQLQRELEQIESQDSFFHEQESS
                     LEKLQELKTKKEDIAKRLIELKEEISRFEGLEGLYHKDAQEIQRKKEKITKGLQEKTA
                     ELYHLANRKEEIQGLLEEYEKGEEELLTIFLALDQAKKALEEEKLPLERKVLRHKRQK
                     VELE"
     gene            complement(1372..2367)
                     /locus_tag="VFD08_05465"
     CDS             complement(1372..2367)
                     /locus_tag="VFD08_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK10502.1"
                     /translation="MHSNIPIFISHQGCPHHCVYCNQHLITGKEALADKKEIAATIEN
                     YLSTLEPQTRKEISFFGGSFSALSQKEQAFYLDIAKSYVGRKGIVGVRFSTRPDAISE
                     EEMDFLSQYPISTIELGVQSLDSEVLRASGRGHGIVDVLRAVNIIRQKKINLGLQMMT
                     GLPEDHYDAAIFTAEKIISLKPDFVRIYPTIVLKKTPLEKMMEQGLYTPWNLEETVEL
                     CSDLLSLFLSAKIPVIRLGLYSSEKNFLDSITAGPYHPALRSLVMGRLYKKALDKLEG
                     SLEIEIHSKEISYLLGHAKENANYFEKKGSPLIYHVNDTLPRNGFLQGGYFVPID"
     gene            complement(2360..3025)
                     /gene="rnc"
                     /locus_tag="VFD08_05470"
     CDS             complement(2360..3025)
                     /gene="rnc"
                     /locus_tag="VFD08_05470"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZK10503.1"
                     /translation="MKNLLKKKEIPYKDIGLYSLAMVHPSYQNEEGLKENNQRFEFLG
                     DAVIDLIVSRYLFTHMGDREGELSRLRAAIVCEGSLAKMAKSLGLEKELLLGKGEELS
                     QGRKRASLLSDCFEAFMGALFMDLGLKEAEKFFLNNYREYMKEVQKGGGYVDYKSKLQ
                     IQCQKDQRSIKYKVLEVSGPDHQRQYKIGLYIDQKLASTGQASSKREAEQIAAGEVLG
                     EDA"
     gene            complement(2988..>3400)
                     /locus_tag="VFD08_05475"
     CDS             complement(2988..>3400)
                     /locus_tag="VFD08_05475"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HZK10504.1"
                     /translation="IEGRQIPEGDVDVIVTDGFTGNVILKFAQGMVKTYGGLLKESML
                     SSLRGKIGALFIKDSLLGFMKKLDYKEYGGAPLLGVKGYLVKAHGSSDSRAIMNALIF
                     MEKYVESAMIEDLEQELLERLKCEELTEEKGDSI"
BASE COUNT          884 a          762 c          604 g         1150 t
ORIGIN      
        1 cttctaactc aactttttgt cttttgtggc gtagaacctt tctttctaga ggaagttttt
       61 cttcttctag ggcctttttt gcttggtcta gtgccaaaaa gatagtaaga agttcttctt
      121 cgcctttctc atattcttct aaaagtcctt ggatctcttc ttttctattt gctaaatgat
      181 atagttccgc tgttttttct tgcagtccct tggtaatttt ttcttttttt ctttgaatct
      241 cctgggcatc tttatgataa aggccctcaa ggccttcaaa gcggctaatt tcttctttaa
      301 gctcaattag tctcttagct atatcttctt tttttgtttt tagttcttgc agtttttcca
      361 aggagctttc ttgctcgtgg aaaaagctat cttgactttc tatttgctct agctctcttt
      421 gaagttgacg ctgtctttct tcttttatgc gcaaatccaa atagttctgg tcacgcaatt
      481 tgattttatc ttgaaggagg tcttggagga ggtcaaattc tttttcaaag ccttctcttt
      541 tttcttccat ctcttctttt tcttcctgca tagaagttct tttctcaaga agtttttcca
      601 gatcatattt actgtgtcca agcaggcgcc caagttcttc ttcttctttt tcatagccaa
      661 gataatcttc ctttgcataa aaaagttcca actccttggc ttttttagaa agttctaaat
      721 agcgcagggc cctatccttc tcctcaagaa gaggttggcg gcgctcatca agttcgccta
      781 gaatgtccat catacgaaga tagtggtctt ctactctggc caggcgcctt ttggcctctt
      841 gctttttata gtggaaaaga ctaatccctg atgcttcttc aaaaatcttt cttctttcat
      901 ctttattttc tttgaggatg tcgtcaatgt ctccttggct aataagagaa tagccactaa
      961 tgcctatggc tgtgtccata aagagctggc gcagatcctt taggcgcaca ggtaggccat
     1021 ttaccttata ggtcgactcc ccactacggt gtagtatacg ctcaatatta agttcatcaa
     1081 gggctcccgg agagagttcc ttcgccccag aaaaaatgag gcggacagag gcaaagtttt
     1141 gaggagctcg atgttttgta cccgaaaaaa tcacatcttc catgcgcctt gcacgtaaag
     1201 atgtggtgct ttgttcgcct aaaacccagc gaatagcgtc tattatattg ctttttccac
     1261 aaccattggg acctacaata caggttaccc caggcaaaaa atcaatatgg gtgcgatcag
     1321 caaaagactt aaagcctttg agttcgatag atttaagaaa catagccgct cctagtcaat
     1381 aggtacaaag taccctcctt gtaaaaaacc attgcgtggt agtgtgtcat ttacatgata
     1441 aatcagtggg ctgccttttt tctcgaaata atttgcattt tccttagcat gaccaagtag
     1501 ataagaaatc tccttggaat gtatttcaat ctccagtgat ccctctagct tatctaaggc
     1561 ctttttatat agcctgccca taacaagact ccttagagcg gggtgatagg gtcctgccgt
     1621 aattgagtct aaaaaattct tttctgaaga atagagtcct agacgaatta ctggaatttt
     1681 ggccgataaa aaaagggaaa gaagatcgct acaaagctcc actgtttctt ctagattcca
     1741 aggcgtataa aggccttgtt ccatcatttt ttcaaggggg gtctttttta aaacgatagt
     1801 aggatatatt cgaacaaaat ccggcttcag agaaatgatt ttctctgcag taaaaatagc
     1861 cgcatcataa tgatcttcag gaagtcctgt catcatttga agccctaggt taatcttttt
     1921 ttggcgtata atattaactg ccctaagaac atcaactatg ccatgtcccc tgccactggc
     1981 gcgtaaaacc tcgctatcta gagactggac ccctagttca atggtggata tgggatattg
     2041 ggataaaaaa tccatttctt cttcactaat ggcatcgggc cgggtagaaa agcgcacacc
     2101 tacaataccc tttcttccaa catagctttt tgcaatatct aaataaaaag cctgttcctt
     2161 ctggctaagg gcgctaaaac ttcctccaaa aaaagaaatt tcctttcttg tctgaggctc
     2221 aagggtagat aagtaatttt ctatggttgc tgcaatctct tttttatcag ccagggcttc
     2281 tttgcccgta attagatgct ggttgcaata aacacagtga tgaggacagc cttgatggga
     2341 gataaatatg ggaatattac tatgcatctt caccaaggac ctcccctgct gctatttgtt
     2401 ctgcctctct tttactggaa gcctgtccag tggaggctaa tttctgatca atatatagac
     2461 caattttata ttgtctctgg tggtctggtc cagagacctc taggacctta tattttatag
     2521 aacgctggtc tttttgacat tggatttgta gcttgctctt atagtcaaca tatccgccac
     2581 ctttttgcac ttctttcata tattccctat aattatttaa gaagaatttt tcggcttctt
     2641 ttaatccaag atccataaaa agagccccca taaaggcttc aaaacagtcg ctaagtaaag
     2701 aggccctttt tcttccctgg ctaagttctt cgcccttacc aagaagtagt tctttttcta
     2761 ggcctaagct ttttgccatc tttgctaggg acccctcgca aacaatggca gccctaagtc
     2821 ggcttagctc tccttctcta tctcccatat gagtaaaaag atatcgacta acaatcaaat
     2881 caattaccgc atcgccaaga aactcaaaac gttggttgtt ttcttttaag ccctcttcat
     2941 tttgatagga aggatgtacc atagcaaggc tataaagtcc tatatcctta tatggaatct
     3001 cctttttctt cagtaagttc ttcacattta agcctttcta gcaattcttg ttccagatct
     3061 tctatcatag cactttctac gtatttttcc ataaatatca gggcattcat aatggctctg
     3121 ctatcgctgc ttccgtgggc tttaacaaga taacccttta ctcctaagag gggagcgccg
     3181 ccatattctt tataatcgag cttcttcata aaccctaaca aggaatcctt tataaataga
     3241 gctcctattt ttccccttaa gctagaaagc atgctttctt tgagtaagcc accataggtt
     3301 ttaaccatac cttgagcgaa ttttaaaata acatttccgg taaaaccatc ggttacaatc
     3361 acgtccacat ccccttctgg gatctgccga ccttcaatac
//
LOCUS       DASRNA010000196         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_135118,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000196 DASRNA010000000
VERSION     DASRNA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135118"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..823)
                     /locus_tag="VFD08_05480"
     CDS             complement(143..823)
                     /locus_tag="VFD08_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HZK10505.1"
                     /translation="MNILIGSVFIAGLLSFFSPCTVPMIPVYIGILADGGDRVKKIGF
                     ISFSPRSMLRTMLFVLGISVSFVTMGFGAGALGALLYSPWFLRILGFIVILLGIKQMG
                     LIRLGFLDKERKVQAKTDVSAYWGAFILGLTFSFGWTPCVGPVLGAVLGISSSGGPLI
                     GGFYMLIYSLGMMIPFLFITLFSSYALSKVEFLERHVFTLQKVGGLLIILMGILLMFD
                     KLSVLSSF"
     gene            complement(827..2068)
                     /locus_tag="VFD08_05485"
     CDS             complement(827..2068)
                     /locus_tag="VFD08_05485"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HZK10506.1"
                     /translation="MKFFKKNRVLQSLGHNAFPKIVCLFLAIATWFIVMENKNPVLET
                     IYRDIPVELIGLEQVENRGLLIESINGDSVDVTVSGRWRDIIKMNGSSIRLSVSLDSI
                     VGKGTANMLIDHRISSAPGVGILSLSKDSISFEVDAIETIKKPINLVLKGELPEELEL
                     GNIVFNDEEIEVTGPSLLLKEIVYLKGELDISSIASTTTEHINLIPVDIDENVVEEVE
                     LEVSNLPVSVPVIGEKTVPLEIVTEGEIRTNYRLTEKTSNLAEVTLKGDTSLLSGISL
                     VRTKPISIKNAYKSFESMLEADLPKDLVVVDAEPLTAKFTIAALDNKRFNFEGKDVVL
                     TGTNESYNYIIDPELILSLEVKDVRENIKNIEEKSITLETDVKGLTPGDYTLQISVKG
                     IAESSHYDVSELPINIVPKGN"
     gene            complement(2065..>2793)
                     /gene="cdaA"
                     /locus_tag="VFD08_05490"
     CDS             complement(2065..>2793)
                     /gene="cdaA"
                     /locus_tag="VFD08_05490"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HZK10507.1"
                     /translation="VYTVISNTRAIQLFKGLVIFFILLRLSSAAELRMVSFVLENVLT
                     YGILALVILFQPELRRALEYLGRSKFFNNMLFRTKTGKDSGVIDSIVEVTLDLSSQKT
                     GTLLVFEGEIGLKDVIETGTELDAEMSSALIKNIFTPNTPLHDGAVVVRGNRIVAAGC
                     LLPLSNDRNVNRDLGTRHRAALGMSAVSDALVLVVSEETGTISIAQNNVLTRFLDKES
                     LLEILVEFYKPKKEGVFQFERSRE"
BASE COUNT          893 a          611 c          474 g          815 t
ORIGIN      
        1 cctcttattt gtgtttctta ctttttgaaa acttgtcctt agtttattca tttatctaat
       61 agaagatagg cctaaaaggg ctagggtctc ttcttctagc acaaagttac tggggaaaaa
      121 cttatttttt cctatcactc tcctagaagc ttgataaaac acttaattta tcaaacataa
      181 gaaggatacc cataaggata attagcaggc ctcctacttt ttgtagggta aaaacatgac
      241 gctctaagaa ttcgaccttg ctaagagcgt aactggaaaa aagcgtgata aataaaaagg
      301 gaatcatcat gccaagggaa taaatcaaca tataaaaacc accaattaag gggcctccag
      361 agctggaaat tccaagtaca gctccaagaa caggccctac acagggggtc caaccaaagc
      421 taaaggtaag ccctagaata aaggcacccc aataggcact aacatctgtt ttggcctgca
      481 cttttctttc cttatccaaa aatcctaagc gaatcagtcc catttgtttt atgccaagaa
      541 ggattacaat aaatcccagg atacgcaaaa accagggact ataaagcaga gctcccaggg
      601 ctcctgctcc aaagcccatg gtaacaaaac ttactgagat ccccaggaca aagagcattg
      661 tccgcagcat agagcgaggg gaaaaggaaa taaaaccaat tttttttacc ctatctcctc
      721 catctgctaa aattccaata taaaccggaa tcatgggcac agtacaagga gaaaaaaagg
      781 atagaagtcc agcaataaaa acgcttccaa ttagtatatt catagattaa tttcctttgg
      841 gtacaatatt tataggaagt tctgatacat cataatggct actttctgct atacctttta
      901 cagatatttg cagtgtataa tctcccggag tcaaaccctt tacatctgtt tccagtgtaa
      961 ttgatttttc ctcaatgttt tttatatttt cccttacatc ttttacttca agacttaaga
     1021 taagctcagg gtcaataata taattataag attcattggt accagtaagg acaacatcct
     1081 taccttcaaa attaaatctc ttattgtcta gggcagcaat agtaaacttt gctgttaggg
     1141 gttcagcatc taccacaaca agatccttag gcagatctgc ttccagcatg gattcaaagc
     1201 tcttataggc atttttaatg cttataggct tggtgcgaac caaactaatt ccactgagaa
     1261 gactggtatc gccttttagg gtcacttcgg ctaaattact ggtcttttct gtaagacgat
     1321 agtttgtacg aatctcccct tcggttacaa tttcaagggg aactgttttc tccccaatga
     1381 ctggcacact gactggtaga ttggacactt ccagttccac ctcttctacc acattctcat
     1441 caatatccac aggaataaga tttatatgtt ctgttgtagt ggatgcaata gagcttatat
     1501 ccagttcccc cttaagataa acaatttcct ttaaaagaag agagggacct gttacttcta
     1561 tttcttcatc attaaataca atgtttccaa gttcaagttc ttctggaagc tctcctttta
     1621 aaacaagatt gataggtttt ttgatggttt ctatggcatc cacctcaaaa gaaatactat
     1681 cctttgaaag agataaaatg cccaccccag gtgccgagct aatcctatga tcaataagca
     1741 tattggctgt acccttgcct actatggaat caagggatac ggaaagacga atggaactcc
     1801 cattcatttt tataatatct cgccatcttc cactaacggt aacatcaaca gaatcaccat
     1861 ttatactctc aataagaaga ccacggtttt ctacttgctc aagaccaata agttccacag
     1921 gtatatcacg ataaatggtc tctaaaacag ggtttttatt ttccataaca ataaaccaag
     1981 ttgctatagc caaaaaaaga caaactattt tcggaaaggc gttatgtccc agagattgta
     2041 gaactctatt ttttttaaaa aacttcattc tctactcctt tcaaattgga aaacaccctc
     2101 ttttttaggc ttatagaatt ctactagaat ttctagcaaa ctttctttgt ctaaaaaccg
     2161 cgtcagtaca ttgttttggg caatactaat ggttcctgtt tcttcactta caactaaaac
     2221 cagggcatct gagacagcac tcatgccaag tgcggcccgg tgcctggtcc caaggtctct
     2281 atttacatta cgatcattac ttaggggcaa aagacatcct gccgccacaa tacgattccc
     2341 gcgcacaacc acagctccat catggagggg ggtgttgggg gtgaaaatat tttttatcaa
     2401 ggccgaggac atttctgcat caagctcggt gcctgtttca atcacatcct taagtcctat
     2461 ctctccttca aagaccaaga gggttccagt tttttggcta ctcagatcaa gagtgacctc
     2521 aacaatacta tcaataacac ctgagtcttt tcctgtcttt gttctaaata gcatattatt
     2581 aaaaaacttg ctgcgcccta gatattccag ggctcgacgt aattcaggtt gaaaaagaat
     2641 aaccagggct aaaataccat aggtcaatac attttctaaa acaaaactaa ccatgcgaag
     2701 ttcagctgca ctcgaaaggc gtagaaggat aaaaaagatc accaaacctt taaaaagctg
     2761 aatcgcacgg gtattactaa taaccgtata aac
//
LOCUS       DASRNA010000197        19027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1109003,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000197 DASRNA010000000
VERSION     DASRNA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 19027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19027
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109003"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFD08_05495"
     CDS             <1..649
                     /locus_tag="VFD08_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase IV"
                     /protein_id="HZK10508.1"
                     /translation="YTKESYIKKINKKTFERNQEQRIKEGDEILKEYQSTSNSELLAF
                     TTKAVVHKLYLDEIEESKASDLGLYLPSLLDLEKDETIVYVTVEEKFEGYVLFGFENG
                     KIAKIDLSAYQTKTRRKKLISAFSLKDKLISILSLKEEEDILLYRYMNQKENVLLINS
                     SIIPSKVTRTSQGVQTLRVGKHGKMTGMERYVGSNLEKYRAKKIPSPGRQRKSFI"
     gene            696..1988
                     /gene="murF"
                     /locus_tag="VFD08_05500"
     CDS             696..1988
                     /gene="murF"
                     /locus_tag="VFD08_05500"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZK10509.1"
                     /translation="MINRIKSGEFAGVFIDTRKPIEGGLYIPIVGDNFNGNSFIPEAF
                     KNGAKAALVERNYYEVNKESLEEFELYPVESGYESLHSFARELRDYVNPYVIAITGSN
                     GKTTTKNMVYSILKKGFDNVYATQGNFNNDIGLPLTILNMPLSTKYLVLEHGIMKKGD
                     IHLLASISRPNLALITNIGTSHIELVGSMEGILKEKLSIADFFTQDNHLIINPFDEML
                     EKARKEGLKATYPEPLELGEIRSDDGYFSFSYRGQEYPLSIRGKHNILNALLAIEASR
                     SLALSDDIIKEGITSYKGEAMRFSEIQVGGIRFINDAYNASPASIIAGLETLVAMKAQ
                     RHIAILGDVLELGVYAQDEHLKLSKHPALKELDAFFTLGDYAGMIGEGHNHAKHFNSL
                     HDLNNYLGEFLKEGDLVLIKASRSIELERILEELKGQL"
     gene            2024..2926
                     /gene="mraY"
                     /locus_tag="VFD08_05505"
     CDS             2024..2926
                     /gene="mraY"
                     /locus_tag="VFD08_05505"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZK10510.1"
                     /translation="MLLIYFLIPLLNRLKLGQHVYELAPDTHQKKQGIPTMGGISFVM
                     VTSLLAFVFIGLKEEHVLVIFSMILFGLIGFLDDSMKLFKEENKGLTAKEKIILQIIA
                     SFFMAFLLGDKGTRILVPFTRYYLNLGILYYPFVAVFLTLLVNSTNLTDGVDGLLASV
                     SFVASLFFLFVTKDLFKPELYRLTLIFLGSLLGYIYYNRFPAKIFMGDTGSMAIGGFI
                     GTMMLLSNTPLFIFFIGFIYVIESLSVVLQVWSYQTRGVRIFKMSPIHHHYELSGYNE
                     NKIVLSFAIVTFVGLLIGLLAYRI"
     gene            2935..4254
                     /gene="murD"
                     /locus_tag="VFD08_05510"
     CDS             2935..4254
                     /gene="murD"
                     /locus_tag="VFD08_05510"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZK10511.1"
                     /translation="MKVLIFGLGRSGRALLDFFKDRSDVWVYDDKEVDIKKLQEEFGE
                     VEFYNPKIDYDLLALSPGPSMDHWLIKSFQEKKVAIKGELEIASQYLKGDILAITGTN
                     GKTTTSSLVKAMVEKQFARSFIGGNIGIPLISYVKDSQEGDVYIVEVSSFQLETIVNF
                     TPKLSALLNITPDHISWHGSMDKYIDAKFRIWENNFPKQIKVINEDDGFLLEEANLRY
                     NKTEDFYRFSVKKKVDKGAYMEDNILYLALDKRKELIRLDELLLTGEHNLSNALAASL
                     IASLYGVKDKFIIEVLKEFTSLEHRYEVLEEKKGRRFINDSKATNVDSALPALRSASR
                     PTVLIAGGMDKKVPLAPLFENWNHAIKHLILFGETKDELAENAQKISAFTDIYIVNNL
                     DEAFSKAMELSSSGWDILFSPACASWDMYESFEIRGKHFKDLYRRLK"
     gene            4251..5348
                     /locus_tag="VFD08_05515"
     CDS             4251..5348
                     /locus_tag="VFD08_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZK10512.1"
                     /translation="MNERINMGILIPVILLTIIGWLLVFSSSAYLSLNQYGTPYYYIK
                     KQTLFICIGFVAMFLTSVVNYKIYNSAPLVILVAIIALVLMALTPILGVEVNAATRWM
                     EIAGQRFMPVDIAKIALVLVMSYTLSHFIVYKNKLVAFIIHMIYPILLIIFTYLQPDF
                     SSMIILFGTMVAILFVGFEPVRYVLLLIGGGFAGLLSLARLAPYRMVRLVGFIEGLKD
                     VDNAMRQIRYGILAIASGGFFGVGPGKSVFNKLWIPEPHNDMILATLGEEFGFVGIVT
                     VLVLYFILILNIIRMVVKLKDPFSRNLTFGMGVIIFLQFVVNFGNTLGILPPTGISLP
                     FISYGGTNIVILMAMVGILLSIHRINWRILS"
     gene            5345..6427
                     /locus_tag="VFD08_05520"
     CDS             5345..6427
                     /locus_tag="VFD08_05520"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HZK10513.1"
                     /translation="MKILLAAGASGGHIYPGLAIIEALEKKSPQIDFLWVGTNKGKER
                     EVAQSKNIPYIPVRAWSINNEGLIWKIQAYWFHGLMTLKLMSKMKREKVDAVVTTGGY
                     ATASSLMAATILGIPIFMHEQNVYPGLGTRKFGKFAKKIFTSFPGTEEHLSGLEEKIF
                     LEGNPIRETFSQLDRDKIRKELGVENKTLVVSVGGSLGAKSINSFIRKLIPKIEKRED
                     LYWVHVVGIDYEEDLTFYKDKKNIEAHLFLNNLPAYLVAADLLISRSGASTLTEISSL
                     GRASILIPSSNVLDDHQMFNAKLFSDKKAALVIEDKKLSCEKSQEILFELLENEGLRK
                     ELAYRSKGIAPFQAAENIAQEILESL"
     gene            6424..7125
                     /locus_tag="VFD08_05525"
     CDS             6424..7125
                     /locus_tag="VFD08_05525"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZK10514.1"
                     /translation="MKKFAFLLLTIIIALGLGTIFLYTDTFILKEIDIESGWIKGEEI
                     LQAAELFEGQNLFYLNKVDIVKSIHQDYRVDEVEFKKIYPGKLLLRITPKKPYVLVSN
                     KGQMLLLDDRGDLIAMDYEYDGLMIIKDFIIEETNLGENLVLKNEELFHTALDLVELC
                     QQAEFKEMTIRQENGFLYLDLADGFRANFGKGSEIVKKFNNFYTIYQDLSEKNVNNGT
                     INLTNPDAPTFLPFD"
     gene            7134..7811
                     /locus_tag="VFD08_05530"
     CDS             7134..7811
                     /locus_tag="VFD08_05530"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZK10515.1"
                     /translation="MNKNYAKIYLPVFIIALLVGLFFNIQGNLIEKGSEDILSRQYQQ
                     ALVDNEELEGQVEFYRKQLAELQKSDEESDKDLSNIDYYKLVSGFTDVKGQGIVIQLS
                     IPDMDQPLNIVDFHRNLLFLMNELNAAGAEAIAIKDERIISTSEISVAGNHIVINGQK
                     LSPPFMIRAIGNPDTLDAALNLRLGYVERLRNNGYFIEVKKSEELEIPKYPILPGFKY
                     AEPLSEN"
     gene            7801..8490
                     /locus_tag="VFD08_05535"
     CDS             7801..8490
                     /locus_tag="VFD08_05535"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZK10516.1"
                     /translation="MKTKLVHFIIIITLIILLFDFGDFFEENQYIPSKEQIIELRDKK
                     LRLEEELDNLKEQVVLYELAIDNEDDLYELLEEEKQRYKTLAGYIPLQGEGLVILMSD
                     SQRVRGEYQDINELIIHDQDVYQIIWELRNAGAEAISINDERFLFHYSKVVCNGPTIR
                     VNGRLYSQPFIIKAIGPRKALQAAVNVYGSYADGLRQKGVFIEANTSVMVEIPAYEED
                     RPLYYLERNEQ"
     gene            8487..8816
                     /locus_tag="VFD08_05540"
     CDS             8487..8816
                     /locus_tag="VFD08_05540"
                     /inference="COORDINATES: protein motif:HMM:NF018632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HZK10517.1"
                     /translation="MIALIVILFLGFVIGYFIPLEVGELVKLYLSLSILVTLSSSLEI
                     LHDLLLDKKNIRKWTIEFTSNLLLSSFLVFLSLRLAIPLYYAIIIYFGSKIFDIMSKI
                     RYSILKE"
     gene            8884..10038
                     /gene="ftsZ"
                     /locus_tag="VFD08_05545"
     CDS             8884..10038
                     /gene="ftsZ"
                     /locus_tag="VFD08_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZK10518.1"
                     /translation="MFDIEERNDLYNFTKIKVVGVGGGGNNAINRMIEADLQGVDFIA
                     VNTDKQDLALVHSEIKLQIGEKITKGLGSGSKPEVGERAAEESRDEIRKSIEGADMVF
                     VTCGMGGGTGTGASPVIAEISREMGALTVGVVTKPFGFEGRKRMAQAEAGIELLKEKV
                     DTLVTIPNDKLLEIVDKKTSLMDSLRVADDVLRQGVQGISDLIVSPGLISLDFADVTT
                     IMKNKGMAHMGIGIGKGESRASDAAKQAVFSPLLETTIEGATGILVNVTGNSTVGLHE
                     TAEALNLITELANPEAEIIYGTSINDNMSDEIKITVIATGFNTKPQKRFTPRTANVQR
                     DDEYHSLRDMKKSMEENQEEVVVPQKEVIELDEWDKDDFRDLPSFLKNRR"
     gene            10070..10531
                     /gene="nrdR"
                     /locus_tag="VFD08_05550"
     CDS             10070..10531
                     /gene="nrdR"
                     /locus_tag="VFD08_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZK10519.1"
                     /translation="MKCPYCGFDVSRVIDSRPTEENNSIRRRRECAECNSRFTTYERI
                     EEINLIVVKRDENREAFNRDKIINGIIRACEKRPVTSKDIEELALRVETQLSNLLKKE
                     ITSEEIGEMVMEELKALDEVAYVRFASVYKSFSDIDTFLDELGKMLVERKK"
     gene            10528..11850
                     /locus_tag="VFD08_05555"
     CDS             10528..11850
                     /locus_tag="VFD08_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HZK10520.1"
                     /translation="MIFPIVAIEEGSIAAQMGIKPGSFLIEINGKEIEDRLDYLFLTT
                     QDYLTVLIEEDGEEVLLEIEKDDDEDLGFIFEHPLMDEARRCHNNCVFCFIDQLPKGL
                     RSSLYFKDDDSRLSFMQGNFVTLTNVGQDQLSRIVDYGIHPINISIHSLDPEIRVKMM
                     GSPKAQDIKKQLDYLYANKVKMNGQIVLVPNFNDGASLYKTLDGLLEYIPHLSSVAIV
                     PVGLSDHRQGLTELRNFTSEEAGEIIEKITLLQKKNINKYGTRFVFLADEFYLLAGKE
                     LPPAQEYEGYVQIENGVGLVRQFIDELHRQMKDITLFPDKRISIVTGKLFGPVLEKEL
                     YSYTLKGLNISVQEIENNFFGEKITVSGLLSYKDLKEQISLEKVDVLLLPSSMLNYDD
                     KSLDDISKKDIVDAFTCDVFFIEPEAKALIDKIKEVSHGTSSSHCRQT"
     gene            11816..13132
                     /gene="der"
                     /locus_tag="VFD08_05560"
     CDS             11816..13132
                     /gene="der"
                     /locus_tag="VFD08_05560"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZK10521.1"
                     /translation="MAPVVAIVGRPNVGKSTFFNKMVGSRISIEEDTPGVTRDRVYGK
                     AKWLEQSFTLIDTGGLDPKSDDLLLSEIRNQAEIAIEVADVIVFMVDARDGLTHDDKE
                     IALILRRTRKPVLLVVNKIDNFKDEHLTYEFYELGFSTIGISSVNILNFGDLLDQILE
                     LLPEEKQSDDDDKTIKMAIVGKPNVGKSSLVNAILGENRVIISDIPGTTREAIETSFE
                     KDGVKYLLTDTAGIRRKKKINEDIEHYSVLRSFSAIERSDITLLMVDAQEGFTEQDKK
                     IIGFAHNEGKAVIVLVNKWDLVEKDNKSYKQWKNKFYAEFPFLRYAPIELISVTEKER
                     LHRILPMVRDVYENSQRRITTGQLNELLNEVVLLHPMPQDKGKSLKIYYATQSQVSPP
                     TFVLFINHEKLMHFSYLRYLENRIRESFDFEGTPIRFVLKEKRKEL"
     gene            13129..14115
                     /locus_tag="VFD08_05565"
     CDS             13129..14115
                     /locus_tag="VFD08_05565"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZK10522.1"
                     /translation="MIIAILGGGSWGTAMAVHLSLIGHEVRLYVRDEKKALKIKSQRK
                     NSEYLSGIILPENIMVDSSLESVLTGVELVIGAIPAQAMESFYQEHPKLLANFPFVNL
                     SKGITRDKLETMEQLYRGYYPQGNYATLSGPSHAEEVARNVPTALVVAGEDENLLKLI
                     QKAFSNEFLRVYRTDDTLGVELSGAFKNVIALVVGMASGCGFGDNTIAALMTRGMAEI
                     MRLGLAMGARQETFLGLSGFGDLIVTCTSDFSRNRKAGKLLAQGYNEEEVKEEVGMVI
                     EGLSTLRSVILLSRKYGQSMPIAETLERVLLANLPIDQAMRKLMTRDYKEEI"
     gene            14112..15281
                     /locus_tag="VFD08_05570"
     CDS             14112..15281
                     /locus_tag="VFD08_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZK10523.1"
                     /translation="MRNKILSTLVITSIIVVLLAFFLNTLGIFEQNVKTYPATMEKLK
                     VELEKEAFVFRDEIIIGALEDGILEYYVEEGERVFAGQPLARLTPSELLEELDSEGNK
                     VPHTFEAPEDLMINIEGIRSNIRSLEDELSYLVSQNKYDEIEDVQERLSSLYLIQSAF
                     KDGKELNISQPITRSSYQNGQHIYYASQPGLVGLTTSSYDQFLNLDNIHLINYSRLED
                     IPESDVLTKAYKGQGFLRIVDNKESFIVAELSEAELSFFQEGRLSTILIDGARISAEN
                     YQILRTEQQTVIVFRIFEDYPAMTSHRNMKVMIIPEETEGILVKTKSIFEQDGQPGVY
                     ILKNNGSKVFLPIKIKGKVGDEVVIYSDYFTLKRGESTNESIETVNLYDEIVEEP"
     gene            15284..15955
                     /locus_tag="VFD08_05575"
     CDS             15284..15955
                     /locus_tag="VFD08_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZK10524.1"
                     /translation="MKQRLEAIFQELETKGKKLGRDISLVAVTKFYDIEKIEESINYG
                     VKVIAENRAQDFLTKAELLDGKVKMHFIGHLQRNKVRIVLPYADLIHSVDSLRLIKEI
                     DKQAKRLDKTQDILLQVNIAKEEQKYGFMKDELEKALELVEKTTNIRVVGMMMMAPFA
                     ENPEEVRVFFKEMSELFDLYKETHYNNVHMDILSMGMSGDYEVAIEEGSNMVRIGSAI
                     YKEEE"
     gene            15955..16455
                     /gene="sepF"
                     /locus_tag="VFD08_05580"
     CDS             15955..16455
                     /gene="sepF"
                     /locus_tag="VFD08_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZK10525.1"
                     /translation="MDSFGNKVKYFFGLEGDDEQEFHEEVDSSPRDELERGTKEERPS
                     ARESQRSYREGRETAASRLIERPVENKLVICKYTPLDHRETMSMIDDARSGRPVIINF
                     QETEDFVAAKIINICEGAAYALDADIMKIASDIFIIVPKGVDVRNHLPSKPEAVEEDN
                     DELDRG"
     gene            16436..16705
                     /locus_tag="VFD08_05585"
     CDS             16436..16705
                     /locus_tag="VFD08_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZK10526.1"
                     /translation="MNLIVGSFLLRLIRALEFLLFVRAISSWFIRNPANPIYQILVML
                     TEPFLSPIRSALSKMQSGGFIDFSILVGYLLLEILSWLVVQSMIA"
     gene            16913..>19027
                     /gene="ileS"
                     /locus_tag="VFD08_05590"
     CDS             16913..>19027
                     /gene="ileS"
                     /locus_tag="VFD08_05590"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZK10527.1"
                     /translation="MYQPLSKENNFDAEKLQRDYWEELNLLQRTLDLREGNKHFVFYE
                     GPPTANGKPGLHHVLARTLKDSICRYKVMQGFYVRRKAGWDTHGLPVEIEVEKRLGIS
                     DKQEIEAYGIAEFNQQCRESVFEYEGYWRRQSEEMGYLIDMDNPYVTLENDYIESVWW
                     ILDQFYKENMMYEGHKILPYCARCGTGLASHEVAQGYKEIKQDTVTVAFKRKDKDEYF
                     LVWTTTPWTLASNVLLSVHPDVSYARVKQGDYVYIIAESLIASVMGEEVEVIETFPGK
                     DLEYVEYEQIMPFMIPDKKAFFVTLADYVTVEDGTGIVHTAPAFGEDDYNTGMAYDAP
                     IIQPVNLEGKYIDTPWKGMFVMDADPEIIIWLRDQGLLYKKQRMEHNYPHCWRCDTPL
                     LYYATSSWYIRMSDLRDELVANNNTVNWYPDYIGEKRFGHWLENVKDWAISRSRYWGT
                     PLNIWTCDCGHQESIASVEELKKRSKTPLEDIELHRPYVDEIVFTCPDCGKDMQRVPY
                     VIDVWFDSGSMPFAQLHYPFEHKEDFHEYFPADFICEGIDQTRGWFYSLMAISTFIMK
                     QSPFKNVLVNDMLLDKEGKKMSKSRGNIVDSFKLFREHGADSVRWYLNFSSPAWQPTR
                     FDTSGLVEVQSKFFGTLKNIYYLFSLYANYDELDVASLEVPYEKRSLLDRWILAKLHK
                     LIDEVTQSYEAYDLTKVTRSIQH"
BASE COUNT         6280 a         2939 c         3928 g         5880 t
ORIGIN      
        1 gtataccaag gaaagctata ttaaaaaaat aaataaaaaa acttttgaga gaaatcaaga
       61 gcagcggata aaagaaggcg atgaaatcct aaaagaatat caatctacta gcaattcaga
      121 gttacttgct tttaccacta aagcagttgt tcacaaactc tacttggatg aaattgagga
      181 gtccaaagca agtgatttgg gtctatattt gccgagtttg ttagatcttg aaaaggatga
      241 aacgatagtt tatgtcactg tggaagaaaa gtttgaagga tatgtgcttt ttggctttga
      301 aaacggcaaa attgcaaaaa ttgatttaag cgcttaccag acgaaaacgc gacgaaaaaa
      361 acttatcagt gccttttccc taaaagacaa attaatctct attttatcgt taaaagaaga
      421 agaagacatt ctgctttatc gctatatgaa tcaaaaggaa aatgttctac tgatcaatag
      481 tagtataatt ccatctaagg ttactagaac tagccagggt gtgcaaacac taagggtagg
      541 caagcatggt aaaatgactg gtatggaaag atatgttggc tctaatttag aaaaatatcg
      601 agcaaagaag attcctagcc caggtcgaca acgcaaaagc tttatttgat agagtcaaat
      661 gtggtatgat aagccttata agtaaggagg ttcttatgat aaatcgtatt aaatctggcg
      721 aatttgccgg tgtttttatt gatacaagaa agcctataga gggtggattg tatataccta
      781 tcgtcggaga taattttaat gggaatagtt ttattccaga agcttttaaa aatggtgcta
      841 aagcagcctt agttgagcga aactattatg aagtaaataa agaaagttta gaggaatttg
      901 agctttatcc tgttgaaagc ggttatgaaa gtttgcatag ttttgcaaga gaacttagag
      961 attatgttaa tccttatgtt atagccatta caggaagtaa tggtaaaaca acaactaaaa
     1021 atatggttta ttctatcctc aaaaaaggtt ttgataatgt atatgctacc cagggaaact
     1081 ttaataatga tataggcctt ccccttacca tattaaatat gcctttatcc accaagtatc
     1141 ttgtcttaga gcacggtatt atgaaaaaag gagatattca tctattggct tcaatttcta
     1201 gacccaatct agctttaatt actaatattg ggactagtca tattgagctt gtagggagta
     1261 tggaaggtat tttaaaggaa aagttatcca ttgctgactt ttttacccag gataaccatc
     1321 tgattattaa cccttttgat gaaatgctgg aaaaagctcg taaagaaggc ttgaaagcaa
     1381 cttatccaga gcctcttgag ctaggagaaa ttaggagtga tgacgggtat ttttctttta
     1441 gctaccgtgg ccaagaatac cctctatcca ttagaggaaa gcacaatatt ttaaatgccc
     1501 tccttgctat tgaggcaagt cgctctttag ctttgtcaga tgatattata aaagaaggaa
     1561 ttacttccta taaaggagaa gctatgcgtt ttagcgagat ccaagtggga ggaattcgtt
     1621 ttattaatga tgcctataat gcatccccag cttcgattat tgctggttta gagaccctgg
     1681 tggctatgaa ggcacaaaga catattgcta tcctaggaga tgttttagaa ttaggagttt
     1741 atgctcaaga cgaacatctc aagctttcta aacatccagc tctaaaagaa ctagatgctt
     1801 tttttaccct aggcgactat gcgggaatga ttggtgaggg acataatcac gcaaaacact
     1861 ttaactcatt gcatgactta aataattatc taggagagtt tttaaaagag ggagatttgg
     1921 ttttaataaa agcatcccga agtatagaac tagaacgtat tctagaggaa ttgaaaggcc
     1981 aactatgaac ttatttttat ttatattgag tggggtattg aatttgcttt taatatactt
     2041 tttaatacct cttttaaatc gtctaaaact gggccagcat gtttatgaac ttgcccctga
     2101 tactcatcaa aaaaaacaag gtattcctac tatgggtggg atttcttttg ttatggtgac
     2161 aagcctttta gcctttgtct ttatcgggtt aaaggaagaa catgtgctag ttatattttc
     2221 aatgatcctc tttggcctta taggtttttt agatgattca atgaaactat ttaaggaaga
     2281 aaacaaagga ttaacggcaa aggaaaagat tattctacaa attatagctt cctttttcat
     2341 ggccttttta ctgggagata aaggaacaag aatcctagtg ccctttacta gatattactt
     2401 gaatttaggg attctttatt atccttttgt ggctgtattt ttaaccctgc tagtaaacag
     2461 taccaatctt actgatggag tggatggtct tcttgcctcg gttagttttg tagcctcctt
     2521 gttctttctt tttgttacaa aagatctttt taagccagaa ctttatcgtt taacccttat
     2581 cttcttaggg tctttactgg gatatattta ttacaatcgc tttcccgcaa agatctttat
     2641 gggagatact ggcagtatgg caattggcgg ctttattggc acaatgatgc tgctgagcaa
     2701 tacacctctt tttatatttt ttattggatt tatttatgta attgaaagtt tatccgttgt
     2761 tcttcaagta tggtcttatc aaactagagg ggtaagaata tttaagatga gtccaataca
     2821 tcatcattat gaactttctg gttacaatga aaataaaatt gttctttcct ttgctattgt
     2881 aacttttgtg ggtctactta tagggctttt agcctataga atttgaggtt gtttttgaaa
     2941 gtattaattt ttggcttggg aagaagtgga agagctttac tagacttctt taaggacaga
     3001 tccgatgttt gggtttatga tgacaaagaa gtagatataa aaaaactgca agaagagttt
     3061 ggagaagttg agttttataa tcccaaaatt gactatgatc ttttggccct tagtcccgga
     3121 ccttctatgg accattggct tatcaagagt tttcaagaaa aaaaagtagc cattaaggga
     3181 gaactagaaa tcgcttcgca ataccttaaa ggcgatattt tagctattac tgggaccaat
     3241 ggtaaaacta caacaagctc tctagtaaaa gctatggtag aaaaacaatt tgctcgatct
     3301 tttattggag gaaatatagg aattcctctt attagttatg taaaggattc gcaagaggga
     3361 gatgtctata ttgtggaagt atctagcttt cagctggaaa caatagttaa ttttacccct
     3421 aagcttagtg cgcttttaaa tataactcca gaccatattt catggcatgg atccatggac
     3481 aaatacatcg atgctaagtt tcgcatttgg gaaaacaact tccccaagca gataaaagta
     3541 attaacgaag atgatggctt tttattagaa gaggccaacc ttcgttacaa taaaacagaa
     3601 gacttttacc gtttttcagt aaagaagaag gtggataaag gagcctatat ggaggataat
     3661 atcctttatt tggctttaga taaaaggaaa gaattgatta gactagatga attacttctt
     3721 actggtgaac ataatctatc aaatgcattg gcagcaagcc taatcgcatc gctttatggt
     3781 gtaaaagata agtttattat tgaggttttg aaggaattta caagtcttga gcatcgctat
     3841 gaagtcctgg aggaaaaaaa aggcagaaga tttataaatg actctaaggc aaccaatgta
     3901 gattctgctt tacctgccct tagatctgcc tctagaccca ctgttcttat tgcaggtggt
     3961 atggacaaaa aagtcccact ggcaccgctt tttgaaaact ggaatcatgc aataaaacac
     4021 ttgattttat ttggagaaac caaagatgaa ctagcagaaa atgcccaaaa gatttctgct
     4081 tttaccgata tttatattgt aaacaatctg gatgaagctt tctcaaaagc gatggaactt
     4141 tctagctccg gatgggatat tctgttttca cctgcttgtg caagctggga tatgtatgaa
     4201 agctttgaga tcagaggaaa acattttaaa gatctttaca ggaggctaaa atgaatgaac
     4261 gaataaatat ggggatttta attcctgtta ttttactgac cataataggt tggcttttag
     4321 tttttagttc ttctgcttac ttgtctttaa atcagtatgg aacaccctat tactatatta
     4381 aaaaacaaac cttattcatt tgtataggct ttgtagcaat gtttcttacc agcgtggtta
     4441 attataaaat atataatagt gcccctcttg ttattttagt tgctataatc gccttagtac
     4501 ttatggctct aacacccatt cttggggtag aagtaaatgc cgctactcgt tggatggaaa
     4561 tagctggaca acgttttatg cctgtggata ttgctaaaat agcccttgtt ttagttatgt
     4621 cctacaccct gagtcatttt attgtttata agaataagct agtcgccttt atcattcata
     4681 tgatatatcc tatactcctg ataatattta cctatctaca gcctgatttc agttcaatga
     4741 ttatactctt tggcactatg gttgctatat tatttgttgg atttgagcct gtaagatatg
     4801 ttttattact aatcggaggc ggatttgcag gtttgttgtc tttagctaga ctagctccct
     4861 atagaatggt tcgacttgtt ggttttatag agggccttaa agatgtagat aatgccatga
     4921 gacaaatacg ctatggtatt ctagctatag cctcaggagg cttctttgga gttggccctg
     4981 gtaagagtgt ttttaacaag ctgtggattc ccgaaccgca taatgatatg attcttgcaa
     5041 ctctagggga ggaatttgga tttgtaggta ttgttacggt gcttgtctta tattttattt
     5101 tgattttaaa tattatacgt atggtagtta aattaaaaga ccctttttca cgcaatctta
     5161 catttggaat gggggtaatt attttcctac agtttgttgt taactttgga aatactcttg
     5221 gtatacttcc gcctacagga atttctcttc cctttataag ttatgggggc actaatatag
     5281 ttattcttat ggctatggtg gggattttat taagtattca ccgtattaat tggaggattt
     5341 tatcatgaag atacttttag cagcaggagc cagcggagga catatttatc ctggcttggc
     5401 aattattgaa gctcttgaaa aaaaatctcc acaaatagat tttctttggg taggaactaa
     5461 taaggggaaa gaaagagaag tggctcaaag taaaaacatt ccctatattc ctgtaagggc
     5521 ttggagcata aacaatgagg gccttatatg gaaaatccaa gcatattggt ttcatggact
     5581 tatgactctt aagttaatga gcaagatgaa aagagaaaaa gttgatgcgg tggtaacaac
     5641 cggaggctac gctacagcct caagccttat ggcagcaaca attttaggga ttcccatttt
     5701 tatgcatgag caaaatgtgt atcctggcct agggacaaga aaattcggta aattcgctaa
     5761 aaagattttc acaagttttc ctggtacaga agaacattta agtggattag aagagaaaat
     5821 atttttggaa ggaaatccca tccgggaaac ttttagtcaa ttagatagag acaagattag
     5881 aaaagaatta ggggtagaaa ataaaaccct agttgtatca gttggtggaa gtcttggtgc
     5941 taagagtatt aattctttta taaggaagct aatccccaaa attgaaaaaa gagaagatct
     6001 ttattgggta catgtggtag gaattgacta tgaagaagat cttacctttt ataaggacaa
     6061 aaaaaatatc gaagcccatc tttttttaaa caatcttccg gcctatcttg ttgcagcaga
     6121 tcttcttatt agtcgaagtg gagccagtac attaacagaa atatccagtc ttggccgcgc
     6181 tagtattcta attccatcct caaatgtttt agatgatcat cagatgttta atgcaaaact
     6241 attttcagat aaaaaagccg ctctagttat tgaggataaa aagttatcct gcgaaaagtc
     6301 acaggaaatc ttatttgaac tcttagaaaa tgagggattg cgtaaggagt tagcctatag
     6361 gagtaaaggt atagcacctt ttcaagcagc tgaaaatatt gcacaggaga tcttggagtc
     6421 cctatgaaaa aatttgcctt tttacttttg actatcatta tagccctagg actaggaact
     6481 atatttttat atacagacac atttatatta aaagaaattg atatagaaag cggatggata
     6541 aagggggaag aaattctcca agcagccgag ctttttgaag gacagaatct tttttattta
     6601 aacaaagttg atattgttaa aagtattcat caggattaca gagtggatga ggttgagttc
     6661 aaaaaaatat atccaggaaa actgctttta aggatcactc caaaaaaacc ctatgtactt
     6721 gtctctaata aaggtcaaat gcttctttta gatgataggg gagatctcat tgcaatggac
     6781 tacgaatatg atggtctaat gataataaaa gattttatta tagaagaaac gaatttaggc
     6841 gagaacttag ttcttaaaaa tgaagaactt tttcacactg ccttggattt agttgaactt
     6901 tgtcaacaag cagaatttaa ggaaatgacc atacgccaag aaaatggttt tttatatctt
     6961 gatctggctg atggctttag ggctaatttt ggaaaaggca gtgagattgt aaagaaattc
     7021 aataatttct atacgatata tcaagattta tcagaaaaaa atgtaaataa tggtacaatc
     7081 aatttaacaa atcccgacgc acccaccttt ttaccctttg attaggaggc attatgaata
     7141 aaaattatgc gaaaatatat ttgcccgtat ttataattgc cctattggtt ggcttgtttt
     7201 tcaatatcca agggaatttg attgaaaagg gtagtgaaga tattttaagt cgtcaatacc
     7261 agcaggccct tgttgataat gaggagctag aagggcaagt agagttttat agaaagcaac
     7321 ttgcagaatt gcaaaaaagt gatgaagaaa gcgacaagga tctatcaaat atagattatt
     7381 ataaacttgt ttcaggtttt acagatgtaa agggacaagg aattgtaatt caacttagca
     7441 ttcccgatat ggaccaacct cttaatatag tggattttca tcgtaacctg ctttttctta
     7501 tgaatgaatt aaacgctgct ggagccgagg caattgcaat aaaagatgag cgcattattt
     7561 ctactagtga aatcagtgtt gctggcaatc acattgttat taatggccaa aaactctctc
     7621 ctccttttat gattagagca ataggcaatc cagatacgct ggatgctgcc cttaatctga
     7681 gattgggata tgttgaaaga ttgagaaata acggatattt tattgaagtg aaaaaaagtg
     7741 aagaactgga aattccaaaa tatccaatat taccaggttt taaatatgcg gagcccctaa
     7801 gtgaaaacta aattagttca ttttatcata attatcactt taatcattct tctttttgac
     7861 ttcggagatt tttttgaaga aaaccagtat ataccttcaa aagaacagat tattgagctt
     7921 agagacaaaa aacttagatt ggaagaagaa ttagacaatc ttaaagagca agttgtttta
     7981 tatgagttag ctatagacaa tgaagatgat ctctatgagc tactagaaga agaaaaacaa
     8041 agatataaaa ccctagcagg ttatattcct cttcaaggtg aagggctagt tattcttatg
     8101 agtgatagcc aaagagttag gggagaatat caggatataa acgaacttat tatccatgat
     8161 caggatgttt atcaaattat atgggaatta aggaatgcag gagctgaagc aataagcata
     8221 aatgatgaac ggtttctttt tcattattca aaggttgtat gtaatggtcc taccattcgt
     8281 gtaaatggtc gattgtattc tcaacccttt ataataaagg caataggtcc aagaaaagca
     8341 ctacaagctg ccgttaatgt gtatggaagt tatgcagatg gactaagaca aaaaggagtt
     8401 ttcatagaag caaacacatc agtaatggtg gagattcctg cctacgaaga agaccgccct
     8461 ctttattatc tagaaaggaa tgaacaatga tagcattaat cgttatttta tttttaggtt
     8521 ttgttattgg ttacttcatc cctcttgagg taggagagct agttaaactc tatctaagcc
     8581 tgtcgatact tgttactctc tcgtcttctt tagaaatact acatgactta ttactagata
     8641 aaaaaaatat aagaaaatgg acaattgaat ttaccagcaa tttgctactg tcgagttttc
     8701 ttgtgttttt gagtttacga ttggcgattc ccctttatta tgccatcata atatattttg
     8761 gctcaaaaat ctttgatatt atgagtaaaa tcagatattc cattttaaag gaataactgt
     8821 ttacataagg gtgaattatc tatataatgg atttaatgaa taggattagg ataaggagga
     8881 gctatgttcg atatagaaga aagaaatgac ctatataatt ttactaaaat aaaagttgta
     8941 ggtgttggtg gtggtggtaa taatgcaata aaccgtatga ttgaagcaga tcttcaaggt
     9001 gttgatttta ttgcagttaa tacggataaa caagatctag ccttagttca tagtgaaata
     9061 aaactacaaa ttggggaaaa gattaccaag ggcttaggtt ctggtagtaa gcccgaagtt
     9121 ggtgaaagag ctgctgaaga aagccgtgat gaaattcgaa agtccattga aggggcagat
     9181 atggtttttg tcacttgtgg tatgggcggt ggaaccggta caggagcatc tccagttatc
     9241 gctgaaatat ccagagaaat gggagccctt acagtgggtg ttgtaacaaa gccttttgga
     9301 tttgagggcc gaaaaagaat ggctcaggca gaagcgggga tagaattact taaagaaaaa
     9361 gtagatacac tagtaacgat tcccaatgat aaacttcttg agattgttga taaaaaaact
     9421 tctcttatgg attcattaag ggtcgcagat gatgttttgc gccaaggtgt tcaaggcatt
     9481 tctgatctta ttgtcagccc aggacttatt tcactcgact ttgctgatgt tacaaccatt
     9541 atgaaaaaca agggcatggc ccatatgggt atcggtattg gcaaaggcga atcaagagct
     9601 tctgatgcag caaaacaagc tgtctttagt ccgcttctag aaacaactat tgaaggtgca
     9661 acaggtatac tagttaacgt aactggtaat tcaactgttg gtcttcatga aacagcagaa
     9721 gccctaaatc taataactga acttgctaat cctgaagctg agattattta cggaacatct
     9781 atcaatgaca atatgagtga tgaaataaag attactgtta ttgccactgg ctttaataca
     9841 aaacctcaaa aaagatttac tcctcgtaca gcaaatgtcc aaagagatga tgaataccac
     9901 tctcttaggg atatgaagaa atcaatggaa gagaatcaag aagaagtagt tgtgccccaa
     9961 aaagaagtta tcgaattaga cgaatgggat aaggacgact ttcgtgattt gcctagcttt
    10021 ttgaaaaaca gaagatagac cctttggcct ttcttctttg gaggaaataa tgaagtgccc
    10081 ttattgtggc ttcgatgtgt ctagggtaat agattctaga ccaacagaag aaaataactc
    10141 catccgtcgt agaagagagt gtgctgagtg caactctcgt tttacgacct atgaacgtat
    10201 tgaagaaatt aatttaattg tagttaagcg tgacgaaaat cgagaagctt ttaatcgaga
    10261 caagattatc aatggtataa tacgtgcctg tgagaagcga ccagtaacct ctaaagatat
    10321 tgaagagtta gccctgcgcg tagaaacaca actttctaac ctattgaaaa aagaaataac
    10381 ttctgaagaa ataggagaga tggttatgga agaattaaaa gctttagatg aagttgcata
    10441 tgtccgcttt gcatcagtct ataaatcctt tagtgatatt gatacttttc ttgatgaatt
    10501 agggaagatg ttagtggaac gcaaaaaatg atttttccca ttgtagcaat tgaagaggga
    10561 agtatagccg cgcaaatggg tattaaacct gggagttttt taattgagat taatggtaaa
    10621 gagatagagg atagactaga ctatctcttt ttaacgaccc aagattatct caccgttttg
    10681 attgaagaag acggtgaaga agttctctta gaaattgaaa aagatgatga tgaagatttg
    10741 ggatttattt ttgaacatcc tttaatggat gaagctaggc gttgccataa taactgtgtt
    10801 ttttgtttta ttgatcaatt gcctaaggga ctgcgaagtt ctttgtattt taaagatgat
    10861 gactccagac tttcttttat gcaggggaac tttgttactt taaccaacgt tggccaagac
    10921 caattaagta ggatcgttga ttatggcatc catcccatta atatctctat tcacagtctt
    10981 gatccagaga ttcgagttaa aatgatggga agtcctaagg cccaagatat caaaaaacaa
    11041 ttggactatt tatatgcaaa taaggtgaaa atgaatggtc aaattgtgct tgttcctaat
    11101 ttcaatgatg gggcttctct ttataagacc cttgatggac tcttagaata tattccccat
    11161 ctgtctagtg tggctattgt acctgtgggc ttaagcgatc atagacaggg tcttactgag
    11221 ttaagaaact tcacttctga ggaagcgggt gaaataattg aaaaaattac actcctacag
    11281 aaaaagaata taaataagta tgggacacgc tttgtatttt tagcagacga gttttatctt
    11341 ttagctggta aagaattacc gccagctcaa gagtatgagg ggtatgtaca gatagaaaat
    11401 ggagtgggtc ttgtaaggca atttatagat gaactccata ggcaaatgaa agatataaca
    11461 ctctttcccg ataaaagaat tagcattgta actggtaaac tctttggacc agtcttagaa
    11521 aaagaattat atagttacac tttaaaggga ttaaatatta gtgtacagga aatcgagaac
    11581 aattttttcg gagaaaaaat tactgtaagt ggactattaa gctacaaaga tttaaaagag
    11641 cagattagtc ttgagaaggt agatgttctt cttttaccct cttctatgtt aaactatgat
    11701 gataagagct tggatgatat ttctaaaaaa gacattgtag atgcttttac ttgtgatgtt
    11761 ttttttattg aacctgaagc caaggcttta attgataaaa taaaggaggt ttcccatggc
    11821 accagtagta gccattgtcg gcagacctaa tgttggaaaa agtacttttt ttaataaaat
    11881 ggtgggatca agaatatcca ttgaagaaga tacccccgga gtaactagag atcgggttta
    11941 tggtaaagcc aaatggcttg aacaaagctt tactttgatt gatactggag gtcttgatcc
    12001 aaaaagtgat gaccttcttc ttagtgaaat acgaaatcaa gcagaaatag ctatagaagt
    12061 agctgatgtg attgttttta tggtggatgc cagagatgga ttaacccatg atgataaaga
    12121 aattgctcta attcttcgcc ggacacgcaa accagtactc ttggttgtca ataaaataga
    12181 taattttaaa gatgaacatc taacctatga attttatgag ctaggatttt ctactattgg
    12241 aatcagcagt gttaatattc taaatttcgg agatttgcta gatcaaatct tggaactttt
    12301 acccgaagaa aagcaaagcg atgacgatga taaaacaata aaaatggcca ttgttggtaa
    12361 acccaatgtt ggtaaatcgt ccttggttaa tgcaatctta ggagaaaacc gagttattat
    12421 atctgacatt cctggaacta ctagagaggc catagaaaca agctttgaaa aagacggcgt
    12481 aaaatatctt cttacagata cagcaggtat tagacgaaaa aagaagatta atgaagatat
    12541 tgaacactac tcagtattac gaagttttag cgcaattgag cggagtgata ttactctttt
    12601 aatggtagat gcacaagaag gatttactga gcaggataag aagattattg gatttgctca
    12661 taatgagggc aaggcagtta tagttcttgt taacaaatgg gatctagtag aaaaagacaa
    12721 taaaagttat aagcagtgga agaataagtt ttatgcggaa tttccctttt taagatatgc
    12781 tccgattgaa cttatttctg taactgaaaa agaaaggtta catcgaattc ttcctatggt
    12841 acgtgatgtt tatgaaaatt ctcaaagaag gataactact ggacaattaa atgaactctt
    12901 aaatgaagta gttcttcttc atcctatgcc tcaggataaa ggcaagagcc ttaagatata
    12961 ttacgctact caaagtcagg tatcaccccc tacttttgtt ctttttatta atcatgaaaa
    13021 gcttatgcat ttttcctact tgcgctatct tgaaaataga atacgagaaa gttttgattt
    13081 tgaaggaacg cctattcgct ttgttcttaa agaaaaacga aaagaattat gattattgca
    13141 attttaggtg gtggtagttg ggggactgct atggcagttc acttaagcct tataggccac
    13201 gaagttagac tttatgtacg tgatgaaaaa aaagccctaa aaattaagag tcaaagaaaa
    13261 aactcggaat atttgagtgg aattatcttg cctgagaata ttatggtaga ttcttctctg
    13321 gaaagtgttc ttactggggt agagcttgta ataggagcaa ttccagccca agccatggaa
    13381 agtttctatc aagaacatcc caagctttta gcaaattttc cttttgttaa tctaagcaag
    13441 ggaattacaa gggataagct agagacaatg gagcaacttt acagaggata ttaccctcaa
    13501 ggtaattatg caactcttag tggtccctca catgccgaag aggtggcaag aaatgtacct
    13561 actgcccttg tagttgctgg tgaggatgaa aacttgttaa agcttataca aaaagctttt
    13621 agcaacgaat ttcttagagt ttatcgcaca gatgatactc taggtgttga actctctggg
    13681 gccttcaaaa atgtaatagc tttagtcgtt ggaatggcaa gtggatgtgg ctttggagac
    13741 aacactatag ctgcactgat gactcgaggg atggcggaaa tcatgcgcct aggactggct
    13801 atgggagcaa ggcaagagac cttcttgggc cttagcggct ttggagatct tattgtaact
    13861 tgtactagcg acttttctcg taaccgcaag gcgggaaagc ttttagccca aggctataat
    13921 gaagaagaag ttaaagaaga agttgggatg gttatcgaag gtctttccac tctaaggagt
    13981 gttatacttc tttcgagaaa gtatggtcag tccatgccta tagccgaaac tttggagagg
    14041 gttcttttag caaatctccc aattgatcaa gcgatgagaa aattaatgac gagggattat
    14101 aaagaagaaa tatgagaaat aaaatactaa gtacacttgt tattacatca ataattgttg
    14161 ttttattggc ttttttcctc aatactttag gtatttttga acaaaatgtc aaaacatacc
    14221 cagctaccat ggaaaaactt aaggtggagt tggagaagga agcctttgtt tttcgtgatg
    14281 aaataatcat tggcgctctt gaagatggaa tacttgaata ctatgtagaa gaaggagaaa
    14341 gagtttttgc tggccaaccc ctagcaagac tgacaccctc ggaacttcta gaagaactgg
    14401 atagtgaggg gaataaagtg ccccacacct ttgaagctcc tgaggatcta atgattaata
    14461 ttgagggaat acgttcgaat atacgaagcc tagaagatga gttatcttat ttagttagtc
    14521 aaaacaaata cgatgaaatt gaagatgtgc aggaacgact aagctctctt tatctgattc
    14581 agtctgcttt taaagatggt aaggaattaa atatcagcca acctataact agatcaagct
    14641 atcaaaacgg tcaacacatt tattatgctt cacaaccagg ccttgttggt ttaacgactt
    14701 ctagctatga tcagttttta aaccttgata atattcatct tataaattat agtagactag
    14761 aagatatccc tgaatctgat gttttaacta aggcctataa agggcagggg tttttgagaa
    14821 ttgttgataa taaagaaagt tttattgttg ctgaactaag tgaagcggaa ctttcctttt
    14881 ttcaagaggg aaggctatct acaattttga tcgatggagc tcgcattagt gcagaaaact
    14941 atcaaatatt aagaacagaa caacaaacgg ttatcgtatt tcgtatattt gaagactatc
    15001 ctgctatgac ctctcacaga aatatgaagg taatgattat ccccgaggaa actgaaggta
    15061 tacttgtcaa aacgaaatca attttcgaac aagatggtca gcctggggtt tatatcttaa
    15121 aaaataatgg aagcaaagta tttttaccta ttaagattaa gggtaaagtt ggagatgaag
    15181 tcgttatata cagtgactat ttcaccttaa aacgtggtga gagcaccaac gaaagcatcg
    15241 aaacagtaaa tttatacgac gagatagtag aggagccctg ataatgaaac aacgattaga
    15301 agccattttt caagaattag aaaccaaggg aaaaaaatta ggcagagata ttagcctcgt
    15361 agcggtgact aaattttatg atatagaaaa aatcgaagaa tctattaatt atggggttaa
    15421 ggtaattgct gagaatagag ctcaagactt tcttacaaaa gctgaacttt tagatggaaa
    15481 agttaagatg cattttattg gacatcttca aagaaataaa gtgcgaattg ttttacccta
    15541 tgctgatcta attcactctg ttgattcact aagacttata aaagaaatcg acaaacaggc
    15601 caaacgcctt gataaaacac aagatattct tcttcaagta aatattgcta aagaagaaca
    15661 aaaatatggg tttatgaaag atgagcttga aaaagcactt gaactagtag aaaaaacaac
    15721 aaatattaga gttgttggca tgatgatgat ggctcctttt gcagaaaatc cagaagaagt
    15781 tagagttttt ttcaaagaaa tgtcggaact ctttgacctt tacaaagaaa cacattataa
    15841 taatgtgcat atggatattc tttcaatggg tatgtccggt gattatgagg tcgcaataga
    15901 agagggaagt aatatggtcc gtattggatc tgcgatctat aaagaggagg agtaatggat
    15961 agttttggaa ataaagttaa atattttttt ggattagaag gcgatgacga acaagaattc
    16021 cacgaagagg ttgactctag ccctagggat gaacttgaac gtggaacaaa agaagaaaga
    16081 ccttctgcca gagaaagcca acggtcatat cgtgagggaa gagaaacagc tgctagtcgc
    16141 ctaattgaac gtcctgtgga gaataaactt gttatatgca agtacacacc acttgatcat
    16201 agagaaacaa tgtcgatgat tgatgatgct cgctcaggtc ggcctgttat tatcaacttt
    16261 caagaaaccg aagactttgt agcagcgaag attattaata tttgtgaagg agctgcttat
    16321 gctcttgatg ctgatataat gaaaatagca agtgatattt ttataatcgt acccaaagga
    16381 gttgatgtga gaaatcatct gccatcaaaa ccggaagcag tggaagaaga taacgatgaa
    16441 cttgatcgtg ggtagtttct tacttcgtct aattcgtgcc ttggaatttc ttctttttgt
    16501 cagggcaatt agttcttggt ttattagaaa tcctgctaat cctatttacc agatacttgt
    16561 tatgcttacc gagccttttt taagtccgat acgaagtgct ctaagtaaga tgcagtcggg
    16621 tgggtttatt gatttttcaa ttttagtagg ttatttatta ctagagattc tgtcctggct
    16681 tgttgttcaa tccatgatag cttaaagaaa acacaattca gattcttctt ttttacagag
    16741 aagtggcggt ggtgcaagcc atgaaaaaga aatctgctat tatcaattct tacaatcctt
    16801 tctcaggcgt tttaccttcg ttacaggtaa ccgaagtggc ctttggctaa tagggtggta
    16861 ccgcgcatat gcgtccctat acctgggctt tttttatatt ataggaggaa tgatgtatca
    16921 accactaagt aaagaaaata attttgacgc agaaaagctg caacgtgact attgggaaga
    16981 gttaaatctt ttacagcgta ctctagatct gcgggagggt aataaacact ttgtctttta
    17041 tgaaggcccc cccacagcca atggaaagcc tggactccat cacgtcttgg ctagaacctt
    17101 aaaagactct atttgtcgct ataaagtgat gcagggcttt tatgtgcgta gaaaagcagg
    17161 atgggatacc catggacttc ccgtggaaat cgaagtagaa aaaagactcg gtatttctga
    17221 taaacaagaa atcgaagcct atggtattgc agaatttaac cagcagtgtc gtgagtcagt
    17281 tttcgaatac gaaggatact ggagaagaca atcagaagag atgggttatc tcattgatat
    17341 ggataatccc tatgttacct tggaaaatga ttatattgag tcagtttggt ggattttaga
    17401 ccagttttac aaagaaaaca tgatgtatga aggtcataaa atcctacctt attgtgctag
    17461 atgtggtaca ggtcttgctt ctcatgaagt agcccagggc tataaggaaa taaaacaaga
    17521 tactgttact gttgctttta aaagaaaaga caaggatgaa tattttctag tatggacaac
    17581 aacaccttgg acccttgcta gcaacgtact tttatcagtt cacccagatg tgagctatgc
    17641 cagagtaaag caaggggact atgtttatat tatagctgag agtttaatcg cctcagttat
    17701 gggagaagaa gtagaagtaa tagaaacctt tccaggaaaa gatctggaat atgtagaata
    17761 cgaacaaatt atgcccttta tgattcctga caaaaaagcc ttttttgtca ctttggcaga
    17821 ttatgtaacc gtagaagatg ggacaggaat tgttcataca gcgccagctt ttggtgaaga
    17881 tgactataat accggtatgg cttatgatgc tccaattatt cagccagtta atcttgaagg
    17941 caaatatatc gacactccct ggaaggggat gtttgttatg gatgcagacc cagaaatcat
    18001 aatctggctt agagatcaag gactcctcta taaaaaacaa agaatggaac acaattatcc
    18061 ccattgctgg cgttgtgata cacccttact ttattatgca acaagcagtt ggtatataag
    18121 gatgagtgat cttagagatg aacttgtagc caataataat acggtcaact ggtatcctga
    18181 ttatattggt gagaaacgtt ttggtcactg gcttgaaaat gtaaaggatt gggccatatc
    18241 aagaagtcgc tactggggaa cgccactaaa catatggaca tgtgattgtg gtcatcaaga
    18301 gtccattgcc agtgtggagg aattaaaaaa acgctctaaa acacccttag aggatattga
    18361 gcttcatcgt ccctatgtag atgaaatcgt ctttacttgc ccagattgtg gcaaggatat
    18421 gcagcgagtc ccttatgtaa ttgatgtttg gtttgattct ggaagcatgc cctttgccca
    18481 gcttcattac ccctttgaac ataaggaaga ttttcatgaa tattttcctg cagactttat
    18541 ttgtgagggg atagatcaaa ctagaggatg gttttattcc ttgatggcca tttctacttt
    18601 tatcatgaag caaagcccct ttaaaaatgt tcttgttaac gatatgcttc ttgataaaga
    18661 gggcaaaaaa atgagcaagt ccagagggaa tattgtagat tcttttaagc tcttccgcga
    18721 gcatggtgct gattcagtgc gttggtatct aaacttttct tcccctgcat ggcaaccaac
    18781 gcgttttgac acatcaggac ttgtagaagt gcaaagtaaa ttctttggaa ccttaaaaaa
    18841 tatatactat ctatttagtt tgtatgctaa ttatgatgag ttagatgtag cgagcttgga
    18901 agttccttat gaaaagagaa gtttgcttga tcgctggatc cttgcaaaac ttcacaaact
    18961 aattgacgaa gttacacaga gttatgaagc ctatgatcta acaaaggtta cgcgcagtat
    19021 tcagcac
//
LOCUS       DASRNA010000198         8266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_135996,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000198 DASRNA010000000
VERSION     DASRNA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8266
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135996"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1477)
                     /locus_tag="VFD08_05595"
     CDS             complement(275..1477)
                     /locus_tag="VFD08_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK10528.1"
                     /translation="MGDIFNNIDKKLFSRTIKDTKKNNINLLQKHKVHSVDFDKKQVY
                     ARDLNTEEEKVFDYDQLVISTGAKSFSPNIKGIDSKNVYTITKPYIVKELKNNLDKYK
                     RVAIVGGGFIGVEVAEQLSKHKHLDLSLYHSRDQLLNKVYDKSAGEAVEKELRRLGLA
                     IYFSERLTDVVVENGQVKEIITSNRQDKVDAVILAIGFRPNTGVFTDKRLKKLKNGAI
                     IIDKYGRTSIENVWSVGDCATVPHKFLKDPYIPLATSANKIGRQIGINLSRNKKELFG
                     PYESISSSSVKVGKLEFGTTGLTEEQAKELGYDYAIAESKVLNRPGFMTDSSGVNFRI
                     IYENKSFKILGARIFGERDAVLRLLPFTTAIHSGLTTKDLEYYDFAYSPPFALTWEAV
                     NTAASVAK"
     gene            complement(1493..1618)
                     /locus_tag="VFD08_05600"
     CDS             complement(1493..1618)
                     /locus_tag="VFD08_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10529.1"
                     /translation="MKVVIIGGVAGGMSTAFKAIRENPKLDITVLEQEDYISFGA"
     gene            1980..3959
                     /locus_tag="VFD08_05605"
     CDS             1980..3959
                     /locus_tag="VFD08_05605"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HZK10530.1"
                     /translation="MDKKSKNSNHKNHHEMMIRDFKKRFFISLIVTFPILILSPMVQE
                     WTNIKLVFLGSNYLLFALATFIYFYGGWPFLKGLIEELREKNPGMMTLIAMAITVAYL
                     YSTATVFGLEGKDFFWELATLIDIMLLGHWIEMRAVLSASAALSKLAELMPNTAHLIK
                     EGEIVEVDISELNSKDRILIKPGEKIPADGMVVKGTSHINESMLTGESIPVEKKQADK
                     VIGGSINGDGSLEVRIENIGEDSYLSKVIDMVEKAQTSKSRTQNLADRAAFWLTIIAL
                     TVGFTTLFVWYAITKDLAYSIARMATVMVITCPHALGLATPLVVASSTAQSAKNGLLI
                     RNRTQFEKARKIDTLVFDKTGTLTHGEFAVNFIEIFDDNYTEDRVIEYAATLESNSEH
                     PIARGIIKKQEEMSLNTLDMTNFQAIKGKGVEGRIGDRDVMVVSPGYLRERKISIPRD
                     LPQHGVSTDVFLIIDNKLIAAIGLSDRIRKQSYKAIKALKEEGIKTWMLTGDNEKTAK
                     QVSQELGLDGYFAEVLPEEKQKKIKELQAEGNFVAMTGDGVNDAPALAQADVGIAIGS
                     GTDVAAETADIILVNSNPEDVVSLIAFAKATYRKMIQNLIWATGYNLIAIPLAAGVLS
                     SVGIIISPAFGAILMSLSTIIVAINAKLLKLEKSS"
     gene            4189..5142
                     /locus_tag="VFD08_05610"
     CDS             4189..5142
                     /locus_tag="VFD08_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK10531.1"
                     /translation="MRKNKGYLLALLVLLLVLASCSPASDTNNKDTINPEDPLVLGSF
                     VDTEGGILGNIVLLTLEENNYMLTDKVQFGTPDVHRNALLEGELDIGIDYTGNGQYYS
                     EGFDEEIWGDAEAGYQFIKTYDKENNQLIWLQAAKANNTEMLAVKKDFAEANNLESLE
                     DFAEYVNKGGEVKFITSQLFAEKNMGLLGMEEAYGFKLQADQLIMLPHGNTAETISAL
                     ANGTDGVNVALVYGTDGSLADLDLLVLKDPLSIPPVFEPSVIIRAEIVEKYPEIKDII
                     EDVFKSLDLEKLQGMNKKVIVDGLSPKDVAKEHLIEEGFLD"
     gene            5163..6314
                     /locus_tag="VFD08_05615"
     CDS             5163..6314
                     /locus_tag="VFD08_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10532.1"
                     /translation="MSRIKDKTLLLLSSIGAAAFVFMNLLEHKPNRIAQGIRYSSYEF
                     FGKLSMVLFLLWLILIALSFYEKKHKDKIVFILTSALLLGLLWGLQLKTGEYIDGQAA
                     ARISLSAGFYIQIFSIYMLFSTYTPRIKKHNYIKWLVFAGIFAFISYFLLRGAFDDLS
                     LIKEYNIKRIQFKDNLRIHAMLTLASVITGAMIAVPLGFLAYANKKFENKIMVPLSII
                     QTIPSLSLFGILLVPLSGLGNLAFFNAIGVSGIGWAPAYIALTLYTLLPIGRNTLTGF
                     YSVDKNVIEAAKGMGMTKRQIVKKIQLPLAFPVIFTGIRIAFIQTIGGAVLAGLVGGG
                     GMGTFVFLGLGEASPDLILLGVLPIVFFTVVLDYILRSFEKTIKGVIYD"
     gene            6307..7236
                     /locus_tag="VFD08_05620"
     CDS             6307..7236
                     /locus_tag="VFD08_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10533.1"
                     /translation="MIKIKNVTKKYDDFTAVKDLSLTVNQSEFLVVLGPSGCGKSTLL
                     RLINKMIPRDGGEIEVKGKNIDEIKGEELRKTIGYVIQSIGLFPHMDVRKNIATVPHL
                     LKWKEKIISARVNEMLSLVGLDPEKYKKKKPSELSGGEAQRIGVARAMASNPDILLMD
                     EPFGAVDPINRLRLQKEFRKIQRRLKKTVVFVTHDIDEALLLSDRICIMNEGEIVQID
                     TPEKMVLYPKNEFVKDFFKGEGILTILSRKPAEDYAKASLLEEEPLDENASLRDALIK
                     ILSTGKDRVALRNGSISWDDILEIVRSDINEIK"
     gene            7223..7957
                     /locus_tag="VFD08_05625"
     CDS             7223..7957
                     /locus_tag="VFD08_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10534.1"
                     /translation="MKSNNKGLILAVFLSVILALIIIYSEEFETVLYKYFSTKSTEGS
                     RTPLLVYFTEHIWMVLLSSLIAVSLGTGLGIFVTSSYGQEFKELLLKTVNLGQAFPSP
                     ALLALAVPILGYGIKGALIALVVYALMPIVYNVVIGIEEVPKDIIDAAKGMGMTEAEI
                     YTKVKIPLALNVILGGIRTALVINISAATLAAAVGAGGLGVLVVNGVRTFDMVLILEG
                     AIPVSLLAIIVDLLFKALEERLIWDA"
BASE COUNT         2679 a         1482 c         1714 g         2391 t
ORIGIN      
        1 gctcttattt attcagtgat acaaacagcc atagcaaatg ggctaaagcc ttaccactat
       61 ctgaactaca tctttgaaga gattcagtta aatgaaaagt tgcaggtggt ggaccttctt
      121 ccctggtcag acaagatacc acagcagtgt aagaaataaa taaagacgat aaaaacatcc
      181 agcatggtca taggcctata gaccagctgg atgtttttct gaggggtgcg cccttggctt
      241 gacgcttact ttttaaagga taaatctact cgctttactt tgcaacagag gcagcagtgt
      301 ttaccgcttc ccaggtcaaa gcaaagggtg gggaataagc aaagtcatag tattctaagt
      361 ccttagttgt aagaccagag tgaattgctg tagtaaaagg aagcaggcgt aggacagcgt
      421 ccctttctcc aaaaattcta gccccaagaa tcttaaagct tttattctca taaatgatcc
      481 taaaattcac tccactggaa tctgtcataa aaccaggcct atttagaacc ttggattctg
      541 ctatagcgta atcatatcca agttctttgg cctgttcttc tgttaggcca gtagtgccaa
      601 attctaactt gcctaccttg actgagctgc tactaattga ttcataggga ccaaagagct
      661 cctttttgtt tctagatagg ttgattccta tttgcctgcc tattttattg gcactggttg
      721 ccagggggat atagggatct tttaaaaact tatgaggaac agtggcacaa tctcccacgc
      781 tccatacatt ttctatggat gtacgaccat acttatcaat aataatagcg ccatttttga
      841 gttttttcag tcttttatct gtaaaaactc ctgtattggg tcgaaatcct atggccaaaa
      901 tcacagcatc caccttatct tgtcgattgc ttgtgattat ttcctttact tgcccatttt
      961 ctaccactac atctgttagc ctctcagaaa aataaatagc aagacccagc cgccttaatt
     1021 ccttttctac cgcttctccc gcactcttat cgtagacctt atttaggagc tgatctcgtg
     1081 agtggtagag gcttagatct aagtgtttat gcttggaaag ctgctcagct acttcaacac
     1141 cgataaatcc accacctaca atagcaaccc ttttatattt atccagattg ttttttagct
     1201 ctttaacgat atagggtttg gttatggtat agacattttt agaatctatc cccttaatat
     1261 ttggactaaa ggactttgcc cctgttgata taaccaactg atcatagtca aagacctttt
     1321 cttcctccgt atttagatcc ctggcataga cttgcttctt atcaaaatcc acagaatgca
     1381 ccttgtgctt ttgcaacaag ttgatattgt ttttctttgt atcctttata gtccttgaaa
     1441 ataacttctt gtcaatatta ttaaatatat caccgatata atagggtaac cctcacgcgc
     1501 caaaggagat gtaatcttct tgttctagta ctgtgatatc cagcttgggg ttttcacgta
     1561 ttgccttaaa ggcagtactc ataccccctg cgactccacc aataataact actttcattt
     1621 catcacctct ctaattattt gtctactacc ttctatttac cctactagtg aaagcttaac
     1681 cttgcagggg aattattatg gactggcaag ctaataagga gacctctcta taaagtttat
     1741 ggagggaccc ctttttccaa caggtcttac taagcgaata ttacaaatgg ggaataaaaa
     1801 gcaaaagagc taggaggatt aaggcaaata ggggagtgca ggcttcattt aggcttatta
     1861 tataaagtga ctgtctgaaa attagaccat aaggaattat tcgtgtagat aatagggctc
     1921 tgggtaaata gactataagg cctacattct taaagatgaa ttagaaagga gacttcctaa
     1981 tggataaaaa aagcaaaaac tccaatcata aaaaccatca tgagatgatg ataagggatt
     2041 tcaagaagag attttttata tcacttattg tcacatttcc tatccttatt ctttccccca
     2101 tggtccaaga gtggacaaat atcaagctag tttttttagg gagcaattat ctgctatttg
     2161 ccttggccac atttatatat ttttatggag gatggccatt tctaaaaggg ctaatagagg
     2221 agttgaggga aaaaaatcct ggtatgatga ctcttattgc aatggcaatt acggtagcct
     2281 atttatatag tactgctacc gtttttgggc ttgaaggaaa ggattttttc tgggaacttg
     2341 ctaccctgat agacattatg cttttgggcc actggattga aatgagggcg gtcttaagtg
     2401 catctgctgc cttaagtaaa ttggctgaac ttatgccaaa tacagctcat ttaatcaaag
     2461 aaggcgagat agtagaggtg gatataagtg aattaaattc taaggacaga atactgataa
     2521 agccaggaga aaaaatcccg gctgatggga tggtagttaa gggaacaagt catataaatg
     2581 aatccatgct aacgggagaa tcaatacctg tagagaaaaa gcaagcagat aaagtaatag
     2641 ggggatctat taacggagat gggtccttag aagtaagaat agaaaacatt ggcgaagact
     2701 cctatttatc aaaggttata gatatggttg aaaaagcaca gacatccaaa tctagaaccc
     2761 agaacctagc agatagggct gctttttggt tgactataat agccctaaca gtgggtttta
     2821 caaccctttt tgtatggtat gcaataacta aggatttggc atactccata gcaagaatgg
     2881 caacagtaat ggttattaca tgtccccatg cactggggct tgcaacccca cttgtggtag
     2941 caagttcaac agcccaatca gccaaaaatg gcttgcttat aagaaataga acgcaatttg
     3001 aaaaggcaag aaagattgac acccttgtct ttgataaaac aggaacacta acccatggag
     3061 agttcgcggt aaattttata gaaatatttg atgataacta cactgaggat cgagtcatag
     3121 aatatgcagc gacactagaa agcaattctg agcatccaat tgcaagagga ataataaaaa
     3181 agcaagaaga aatgagtcta aatactctgg acatgaccaa cttccaagct attaaaggaa
     3241 agggtgtaga aggaaggatt ggtgacaggg atgtaatggt tgtaagtccc ggttatttaa
     3301 gagaaagaaa gattagcata cccagggacc tgccccagca tggggtatca acggatgtgt
     3361 ttttaattat tgataataaa ttaatcgcag ccataggcct ctcggataga atcaggaaac
     3421 agtcatataa ggcaataaaa gccttaaaag aagaaggaat taaaacatgg atgctaactg
     3481 gtgacaatga aaaaactgcc aaacaagtat cgcaagaact gggcctagat ggatactttg
     3541 cagaagtatt accagaagaa aaacaaaaaa agataaaaga actccaagca gaaggaaatt
     3601 ttgtcgcaat gactggagat ggagtaaatg acgccccagc actggcccag gctgatgttg
     3661 gtatagcaat cggttccggt acggatgttg cagcggagac ggcggatatt atacttgtaa
     3721 attccaatcc agaagatgtg gtatctttaa tcgcctttgc caaggccacc tatagaaaaa
     3781 tgatacaaaa cctcatctgg gcaacgggat acaatctaat agcaatccca ctggcagcgg
     3841 gggttttatc atcggtagga attattatct ctccggcctt tggagccatt cttatgtctc
     3901 taagcacaat tatagtagcg atcaatgcaa aacttcttaa attagaaaaa agtagttaaa
     3961 tcttttctta gaaaagtttc agattgacac cataaaaagg gcttaattga taggggggtg
     4021 cagtaagact caaggggact taggttgata tgtaaattaa agggtaaaaa agctctatag
     4081 ggcaaatctc ttgacttaaa tcaagaaaat aagcttgtaa gactaaaaga aacagtcatt
     4141 tatagagaag tttaaataaa cagaagaaaa agcaaatggg aggaaaagat gagaaaaaat
     4201 aaaggatatt tactagcctt gcttgtgtta cttctagtac tagccagttg tagcccagct
     4261 agtgatacaa ataataagga cacaataaat ccagaggacc cactagtact tggctccttt
     4321 gttgatacag agggtggaat tcttggcaat atagtacttt tgaccctgga agagaataat
     4381 tatatgctta cagataaagt gcaatttggg acccctgatg ttcatagaaa tgcactacta
     4441 gagggtgagc tagatattgg aattgactat acaggcaatg gacaatatta ttcggaggga
     4501 tttgacgaag aaatttgggg cgatgcagag gcaggctatc agtttataaa gacctatgat
     4561 aaagaaaaca atcaattaat ctggctacaa gctgcaaagg ctaataatac tgaaatgttg
     4621 gcagtaaaaa aagactttgc tgaggccaac aatctagaat cactcgaaga ttttgccgag
     4681 tatgttaata aggggggaga ggtgaaattt atcaccagtc agctctttgc agaaaaaaat
     4741 atgggcctgc tggggatgga agaggcttat ggatttaagc tccaggcaga tcaactgatt
     4801 atgcttcccc atggaaatac agcagaaaca atcagtgccc tggcaaatgg aacggatggt
     4861 gttaatgtgg ccctggttta tggcaccgat ggttcactag cagacctgga tttactggta
     4921 ctaaaagacc cactttctat tcctcctgtc tttgaacctt ctgtaatcat cagagcagag
     4981 atagtagaaa aatatccaga gataaaagat ataattgagg atgtatttaa aagcttggat
     5041 cttgaaaaac tccaaggcat gaataaaaaa gtaattgtgg acggcttatc accaaaggat
     5101 gttgcaaaag aacatctaat agaggaaggc tttttagact agttttctca aaaggtaggt
     5161 aaatgagtag aataaaagac aaaacattgc tgctgctatc ctccatagga gcggcagcct
     5221 ttgtttttat gaatttacta gaacataaac ccaatagaat cgctcaaggc ataagatact
     5281 cttcttatga gttttttggc aagttaagca tggtcctttt tcttttatgg cttatactaa
     5341 ttgccttatc tttctatgaa aaaaaacaca aggataagat tgtttttatc cttacctctg
     5401 ctttacttct aggtttactt tggggtttgc agttaaaaac gggggaatat attgatgggc
     5461 aagcagcagc aaggataagc ctttctgctg gcttttatat tcaaattttt tccatatata
     5521 tgctcttttc cacttatact cctaggatta aaaagcataa ctatatcaag tggctggtat
     5581 ttgcaggtat atttgcattt ataagttatt ttttgcttag gggtgccttt gatgatttat
     5641 cattaatcaa agaatacaat attaaaagaa tccagtttaa ggacaatttg agaattcacg
     5701 ccatgttaac cttggcttct gttataacag gtgccatgat tgctgttccc ttgggatttt
     5761 tagcctatgc aaataaaaag tttgaaaata aaatcatggt cccccttagt ataatccaaa
     5821 ccatcccaag tctttctttg tttggcatcc ttttagttcc cctatcagga cttggcaatc
     5881 tagccttttt taatgcaata ggagtaagtg gaattggatg ggcccctgct tatattgcac
     5941 tgacccttta taccctcctg cccataggaa gaaatacttt gacaggattt tattccgtgg
     6001 ataaaaacgt aatagaagct gcaaagggaa tgggtatgac taagaggcaa atagtaaaaa
     6061 agattcaatt gcccctggct tttcctgtta tatttactgg cattcgaatt gcctttattc
     6121 aaacaatagg cggagcagta ttagcagggc ttgtaggtgg gggaggtatg ggcacctttg
     6181 tatttttagg cctaggggag gcctcacctg acttaatcct tctaggggtg cttcctattg
     6241 tcttttttac tgtagtgctc gattatattt tgaggagctt tgaaaaaact ataaagggtg
     6301 ttatatatga ttaaaataaa aaatgtaaca aagaaatatg atgattttac tgcggtaaaa
     6361 gatttaagtt taacagtaaa tcagtctgaa ttcctagtag tactgggtcc ttcaggttgt
     6421 ggcaaatcaa ccctacttag actaatcaat aaaatgattc ctagagatgg tggggagata
     6481 gaggtcaaag gtaaaaacat agatgagata aaaggagagg aactaagaaa gaccattggc
     6541 tatgttattc aaagtattgg actatttcct catatggatg taagaaagaa tattgctacc
     6601 gttccccacc tactaaagtg gaaggaaaaa ataatttctg caagagtaaa tgaaatgcta
     6661 agcttagttg gcttggatcc agaaaaatac aaaaagaaga aaccctcaga actttccgga
     6721 ggagaagcgc agaggatagg tgttgctagg gcaatggctt ctaatcctga tatcctgctt
     6781 atggatgaac cctttggagc ggttgaccca atcaatagat tgagacttca aaaagaattc
     6841 agaaaaatcc aaagaagact gaaaaaaaca gtggtttttg taacccatga tatcgatgag
     6901 gccctgctct tatcagatag gatttgcata atgaatgaag gggagatagt gcaaattgat
     6961 acacctgaga aaatggtcct ttatcccaaa aatgaatttg ttaaagactt ctttaaagga
     7021 gagggtattt tgacaatttt atcgagaaaa cctgcagaag actatgccaa ggctagccta
     7081 ttagaagaag agccactaga tgaaaatgcc agcttaaggg atgctttaat aaagatccta
     7141 agcacgggca aggatagagt cgctcttaga aatggaagta tttcctggga tgatatttta
     7201 gaaatagtta gaagtgatat aaatgaaatc aaataataaa ggattaatcc ttgcagtctt
     7261 tttatcagta atacttgccc ttatcattat atactcagag gagtttgaaa cagtgctcta
     7321 taaatatttt agcaccaaaa gcacagaggg ctctagaacc cccctgctgg tttactttac
     7381 cgagcatatt tggatggttt tattgtctag ccttattgca gttagcctgg gaactggttt
     7441 ggggatcttt gtcacttcaa gctatggcca agagtttaaa gaactcttat taaaaacagt
     7501 aaatttaggc caggcatttc catctccagc cctgctggcc ctggcagttc caatattagg
     7561 atatggaata aagggagcct taatagccct agtggtatat gctttaatgc ccattgtata
     7621 taatgtggtt attggtatag aggaagttcc aaaagatatt atagatgccg caaagggcat
     7681 gggtatgaca gaagcagaga tatacaccaa ggtaaaaata cccctagcct taaatgtaat
     7741 cctaggtgga ataagaacgg cccttgttat aaatataagc gcggccactc tagcagctgc
     7801 tgttggagca ggtgggctgg gagtcctggt tgtaaatgga gtaagaactt ttgatatggt
     7861 gcttatatta gaaggggcca ttcctgtgag cctacttgct atcattgtag atcttttatt
     7921 taaagcccta gaagaaagac taatttggga cgcctaaagc cctaggtctc ttaaggcctg
     7981 ctatagccag aaaactccaa aaacacatat caagtcataa gaaaaaaaag cccccctaag
     8041 ctggagctaa gcccatctaa ggaggtcgtt tttttaccgc caaactagtt tattttaaac
     8101 tactggaatt gtttgaaaaa ctcagtcatt ttctccattc cagctttttc aacttctgga
     8161 ctattgcaat aacctattct aaggaatcct tccacatcaa gggcggaccc tggcacaagc
     8221 ataactcctg tttcttctag cagcttttcg caaagctcca cagagg
//
LOCUS       DASRNA010000199         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_67539,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000199 DASRNA010000000
VERSION     DASRNA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67539"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFD08_05630"
     CDS             complement(<1..208)
                     /locus_tag="VFD08_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10535.1"
                     /translation="MSKSVKDVIIGTMENGPELLNELAMAKKIGSDIEVQRGDVGRKL
                     GQYHPPFINLKVVEKETLNPVAVRL"
     gene            complement(210..1742)
                     /locus_tag="VFD08_05635"
     CDS             complement(210..1742)
                     /locus_tag="VFD08_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK10536.1"
                     /translation="MSNYDYIIIGAGNAGLASAATLTQAGKKVALFEKHNIPGGCGTT
                     FRRGRFEFEVALHQLNSMNTADNPGPVRKLLRKWEVEDKIDWIKIDSLYKINLPGGRG
                     VALPSDKDQMCELLQSEFPLEKDKIKEYLDMVWRFNEELGDFLGQSENSSPEASELKK
                     LVLKFGFPKKYPTLQKYAARSSQDVLDEFFESKELQLCVSAYWCFMGMPLERFPFSIL
                     SRCMYLYTIDNPYYLKGGSQMISQGLCETIRKYGGQIFFNNGIKEIIIENNQARGVID
                     EEGNSYYASHIISNISPIETYAGLCKAEDVPQQARDYLKSYTVGISAFTIFMGLDCPP
                     EEIGFTDSFNLIYDSLNPNEDFKNAYHLDSSVDPIIATCYTIDDPTLSDEGTSIMTAG
                     CLKYGEAFMQLDPKDYYEKKYEIAEQIIDRLETRFPGIKDHIEEIEVATPLTHMRYLQ
                     HPGGAIYGFEQDLKSAVFFYPQEEFVDGLHFAGGWVNTCGFGPNYVYGNKVAENLLKE
                     EV"
BASE COUNT          609 a          454 c          392 g          721 t
ORIGIN      
        1 taaggcgaac tgctacggga ttaagggtct ctttttccac tacctttaaa ttgataaagg
       61 gagggtggta ttgtcccagt ttccggccaa catcgccccg ctgaacttca atatcacttc
      121 ctattttttt ggccatggcc aattcattta ataattcagg accattttcc atggttccta
      181 taatgacatc ctttacagat ttactcatct tatacctctt ctttcagtag gttttcagct
      241 actttattcc catacacata atttggtcca aatccacagg tattgaccca accgccggca
      301 aagtgaagtc catctacaaa ttcttcttga ggatagaaaa agactgctga ttttagatcc
      361 tgttcaaagc cgtagattgc tccacctgga tgttgcaaat acctcatatg ggtaaggggt
      421 gtggctactt caatttcttc aatatgatcc ttgatgccgg gaaaacgtgt ttctaggcgg
      481 tcaataattt gctctgcaat ttcatatttt ttctcataat aatcctttgg atcaagttgc
      541 ataaaggctt cgccatattt tagacaacct gctgtcataa tacttgttcc ttcatcacta
      601 agagttggat catcaatggt ataacaagta gcaatgattg gatccacaga agaatctaaa
      661 tgataggcat ttttaaagtc ttcattgggg tttagtgaat catagattag gttaaatgaa
      721 tcggtaaaac ctatttcttc aggtgggcaa tctagtccca taaagatggt aaaggcacta
      781 atgccaacag tatagctctt taaataatcc ctagcctgct ggggtacatc ctctgcttta
      841 caaaggcctg cataggtttc aatgggtgaa atgtttgaga taatatgact agcatagtag
      901 ctattgccct cctcatcaat gacacccctg gcttgattgt tttcaattat gatttcttta
      961 attccgttat taaaaaagat ttgaccccca tattttcgaa tagtttcaca aaggccctga
     1021 gaaatcattt gagaaccacc ctttaagtag tagggattat ctattgtata tagatacata
     1081 cagcgtgaaa ggatggagaa gggaaaacgc tccaggggca tacccataaa gcaccagtag
     1141 gcactcacac acagttgtag ttccttgctc tcaaagaact catctaggac atcttggctg
     1201 ctacgggcag catatttttg tagggttgga tattttttgg gaaagccaaa ctttaaaact
     1261 agttttttga gttcacttgc ctcaggactg ctattttcgc tttgccctaa aaagtcaccc
     1321 agttcttcat taaatctcca gaccatatca aggtattctt tgatcttgtc tttttcaagg
     1381 ggaaactcac tttgaagtag ctcacacatc tggtctttat cactgggtaa ggcaacccct
     1441 cttccccctg gtagattgat tttgtataaa ctatcaatct ttatccagtc tatcttatct
     1501 tctacttccc attttctaag tagttttctt acgggccctg gattgtctgc ggtattcatt
     1561 gagtttaact ggtgaagggc tacctcaaat tcaaagcgcc ctcttctaaa ggtagtacca
     1621 caacctccag gtatattgtg tttttcaaac aaggcaacct ttttgcctgc ttgggttagt
     1681 gtggcagcag atgcaagtcc tgcattacct gccccaataa taatgtaatc gtaatttgac
     1741 atttaaaact cctttctttc ttaatttgcc aaagtatgca ataaagcaat aggcaataag
     1801 taccaagttg acaatcatca aaatcaattg cacttgcttt gtttaacttt agtgataaag
     1861 cgacttagtt tttccttttc ccatttcttt tccaaaatat ctttatttgc gatttgaatg
     1921 agcgtgccta ttgtaagtga attgacccga aggaataagc aaatccctgg accttaaaga
     1981 gtggcctcgt tgggtaatgc aggctaaata taaaccacta taggggcccc ttgtatcggc
     2041 cttgcaaact acctctaagc caaggacaag aaaagctctt ctttagctat tttaaaatat
     2101 ctgttccagt gaggctttaa taagacttca ctttggttta accagtttgt tattttttta
     2161 ttacactatg attcta
//
LOCUS       DASRNA010000200         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_961479,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000200 DASRNA010000000
VERSION     DASRNA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961479"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFD08_05640"
     CDS             <1..263
                     /locus_tag="VFD08_05640"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HZK10537.1"
                     /translation="LGLYGGKGKPEEEKNPKLNEAIREYIDWFKESQGSLECMKILGQ
                     ETFEAMRRGVYPVNCGNIMSAGYRKLREIIAEYDFTIRGSND"
     gene            256..1584
                     /locus_tag="VFD08_05645"
     CDS             256..1584
                     /locus_tag="VFD08_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK10538.1"
                     /translation="MTDKRLVTESLCPVCLKRIEAKKVREGDQVFMVKTCPEHGDFKT
                     IIWRDDPNYGSWRRSKTPAFPAKAFTQVDKGCPFDCGLCPRHRQHTCTALIEVTERCN
                     LDCSFCFADAGGNYGPDMETIGFYYDRIMESSQGCNIQISGGEPTMREDLPRIIRLGH
                     KKGFRFIQLNTNGLKLADYDYVLKLKEAGLNSVFLQFDGLDDGIYEKLRGARILEKKL
                     KAINNLCKAGIGTILVVTVVPGINDHCLYDIVKFGSENIPGVRGVHFQPVSYFGRIPK
                     IPTDEDRITLPEVMSKLSDQSDGTIDIKDFKAPCCENSYCSFNGSFIVRDDKLVAIGN
                     NRKRPSEKSLEKIQDGAEGSAKAKSFVEKNWAIREQRQDNDKYVSFTKLLNRIEHNRF
                     AISAMGFMDAWDFDMERIRGCCIHVVSKEGNMIPFCAYNVTNMEGKSLYR"
     gene            1601..2908
                     /locus_tag="VFD08_05650"
     CDS             1601..2908
                     /locus_tag="VFD08_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZK10539.1"
                     /translation="MLSFYEKWIRGLVLEGDQPFNRDAIEAYQLKQLKKTLFYVKDKS
                     KFYANQLRNLDVESIQNLDDFSAQVPFTYPEDLARESWSFLCLAQHEIARIITIRTSG
                     TKEEKRIFFTKQDLERTIDFFHHGMAYLIRPGQKVLVLMPGNYYGSIGDLLKKGLARL
                     GCDTLIMGAGKNSLSVLNTIKREAIDSVVGLPQELYQLARIKNNLAEMNDLKLETILL
                     SADYVPSSVKEVLEKAFCCKVFNHYGMSEMGYGGAVDCGCLGAYHLREADLYFEIIDP
                     ISGQVLPPGQEGEVVFSTLNREAMPLIRYRTGDRASFIKGGCPCSKLLKKMSHVKGRY
                     QNLLYIDNQPLSMALLDELLFRMDNLLDYRASLKAGKILSLSLKAVDENKPLGIEEVR
                     RALEMGPFEKLLREQKLILEVNDEGQWEYVETTMQKRVLKIID"
BASE COUNT          873 a          462 c          731 g          862 t
ORIGIN      
        1 ttttaggcct ttatgggggt aagggcaagc ctgaggaaga gaagaaccct aaactaaatg
       61 aggctataag ggagtacata gactggttta aagaaagcca aggtagtctt gaatgcatga
      121 agattttagg gcaggaaacc tttgaggcga tgcggcgggg ggtctacccg gtgaattgcg
      181 gcaatataat gtctgccggt tataggaaat taagagaaat cattgctgag tatgatttta
      241 ctataagggg atcaaatgac tgacaagaga ttagtgactg agtccctttg tcctgtctgc
      301 ttaaagagaa tagaggcgaa aaaagttaga gagggtgacc aggttttcat ggttaagacc
      361 tgtcccgagc atggtgattt taaaaccatt atctggcgag atgatccaaa ttatggaagt
      421 tggcggagat ccaaaactcc ggcctttcca gccaaggctt ttactcaggt ggacaaggga
      481 tgcccttttg attgtggcct ttgtccccgt catagacaac atacctgtac agcactcatt
      541 gaggttacag agcgttgtaa tctggattgt agcttttgct ttgccgatgc gggaggaaac
      601 tatgggccag atatggagac catcggtttt tattatgacc ggattatgga atctagtcag
      661 ggctgcaata tccaaatttc tggcggtgag cccaccatga gggaggatct gcctcggatt
      721 attagactgg gtcataaaaa gggttttcga tttattcagc tcaataccaa tggacttaag
      781 cttgccgatt atgattatgt actaaaactt aaagaagctg ggctgaactc cgtcttttta
      841 caatttgatg gtctcgatga tggaatctat gaaaaattaa ggggagctag gattcttgaa
      901 aaaaagctta aggctataaa taatctttgc aaggctggta tcggtactat tctggtggta
      961 acagtggtgc ctgggattaa tgatcactgc ctttatgata tagtcaaatt tggatctgaa
     1021 aatattcctg gcgttagagg ggttcatttt cagcctgtca gctattttgg taggattcca
     1081 aaaattccca cagatgaaga taggataacg ttacctgaag tgatgagtaa gctctcagat
     1141 caaagtgatg ggacgataga tattaaagat tttaaagcac cgtgttgcga gaattcatat
     1201 tgctccttta atggaagctt tattgttcgt gatgacaaac ttgtggctat tggaaacaac
     1261 agaaagagac cttctgaaaa gagtctggag aagattcaag atggggcaga gggatcggcc
     1321 aaggccaagt cctttgttga gaaaaattgg gccattcgag agcaaagaca agataatgac
     1381 aagtatgtct cttttactaa gttactaaat aggatagaac acaatcgctt tgccatatct
     1441 gccatgggct ttatggatgc ctgggatttt gatatggaga gaatcagggg ctgctgtatc
     1501 catgttgttt ccaaagaagg taatatgatt cctttttgtg cctacaatgt gaccaacatg
     1561 gaaggtaaga gcctttatcg atagcctcta ggagtttgaa gtgcttagtt tttatgagaa
     1621 atggattagg gggctggtgc tggaaggtga ccagcctttt aatagggatg ccatagaggc
     1681 ctatcagctg aagcaattaa aaaagacgct tttttatgtt aaggataaaa gcaaatttta
     1741 tgcgaatcag cttaggaatc ttgatgtaga aagcattcaa aacttagatg atttttcagc
     1801 ccaagtgcct tttacctatc ctgaagattt agctagagaa tcttggagtt ttctctgtct
     1861 agcccaacat gagatagcta gaattataac catcagaact tcgggtacga aggaagagaa
     1921 aagaattttc tttacaaagc aagaccttga gaggacgata gatttttttc atcatgggat
     1981 ggcttatctg attagacctg gtcaaaaggt cttggttttg atgcctggaa attactatgg
     2041 tagtatcggc gatctcctta aaaaaggtct tgctaggctg ggctgtgata ctttgattat
     2101 gggggctgga aaaaatagtt taagtgtttt aaataccata aagagagagg ctattgatag
     2161 cgtggttggc cttcctcagg aactttatca actggctaga attaaaaata atttagcaga
     2221 gatgaatgat ttaaaactag agactatcct actaagtgca gattatgtgc cgagttctgt
     2281 taaggaagtt ttggagaagg ctttttgttg taaggtcttt aatcactatg gtatgtctga
     2341 gatgggatat ggcggggctg tggattgtgg ctgcctaggt gcctatcatt taagagaagc
     2401 cgacctctat tttgaaatta ttgaccctat tagtggtcaa gttcttcctc cagggcagga
     2461 aggtgaagtt gttttttcaa ctcttaacag agaggctatg cccctcatcc gttatagaac
     2521 tggcgataga gctagtttta ttaaaggggg ctgcccgtgt agtaagctgc ttaagaagat
     2581 gtcccatgta aagggtaggt accagaatct tttgtatata gataatcagc ctttatctat
     2641 ggctctttta gatgaactcc tctttagaat ggataatctt ctagattaca gggctagtct
     2701 taaagcagga aaaattctga gcttatcact taaggcggtg gatgaaaata aacctcttgg
     2761 cattgaagaa gtgagaaggg ctttggaaat ggggcctttt gaaaagcttt taagagagca
     2821 aaaattgatt cttgaagtaa atgatgaagg tcagtgggag tatgttgaaa cgaccatgca
     2881 aaaaagagtt ctaaaaatta tcgactagcc atgagaatgg tatttatg
//
LOCUS       DASRNA010000201         6043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_438788,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000201 DASRNA010000000
VERSION     DASRNA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438788"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..205)
                     /locus_tag="VFD08_05655"
     tRNA            complement(131..205)
                     /locus_tag="VFD08_05655"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Cys,seq:gca)
     gene            complement(222..297)
                     /locus_tag="VFD08_05660"
     tRNA            complement(222..297)
                     /locus_tag="VFD08_05660"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Lys,seq:ttt)
     gene            complement(301..377)
                     /locus_tag="VFD08_05665"
     tRNA            complement(301..377)
                     /locus_tag="VFD08_05665"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Met,seq:cat)
     gene            complement(467..1033)
                     /locus_tag="VFD08_05670"
     CDS             complement(467..1033)
                     /locus_tag="VFD08_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZK10540.1"
                     /translation="MKKSILFAGVGGQGILLASTILGAAVMEAGYDIKMSEVIGMAQR
                     GGSVTTQLRFGDKVDSPLISLGEADVLVSFELMEALRWSEYLRPGGKAIINDYKLPSA
                     PILTGDTKYPEGIKKHLIKGFDCHFLDASNKAIELGNIRVQNIILLGALAKALDLKEI
                     DWPSYVNKYVKEEFREINLKAFDLGYNS"
     gene            complement(1034..2812)
                     /gene="iorA"
                     /locus_tag="VFD08_05675"
     CDS             complement(1034..2812)
                     /gene="iorA"
                     /locus_tag="VFD08_05675"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZK10541.1"
                     /translation="MKKLMTGNEAIARGVYEAGVVYASAYPGTPSTEILENLAHHKDI
                     VAEWAPNEKVALESALGASMAGVRALAAMKHVGVNVAADPLMTMGYTGITGGFLLVSA
                     DDPGCHSSQNEQDNRYYAKFAKIACLEPSTPQECLDFMREAYKISESYDSPVLIRMTT
                     RVCHSKGLVETGLRKEVEPTAYTRQASKYIANPGNARIRRPMVEERLQKLADYSESSP
                     LNVLEDYGNTLGIITAGVSYSYVKEVFGDSADILKIGFSFPLPIKKIERFIASHDKVY
                     VVEELEPFMEEQIKAAGLSVIGKELIPSMGELNPDIIKEKLLGEVAETIKADPELLVG
                     RPPILCAGCPHRGFFYELGKKRNVVITGDIGCYTLGGAPPLNATDSVICMGASLSMGH
                     GAKKALEKTGSDKKVVGVIGDSTFLHSGMTSLLNAVYNNSSTVNVILDNRVTAMTGHQ
                     QNPGTGYTLQEDPANQVDLEAIVRALGVKKVLRVNPLMLEETRSAINEALEYDQGPSV
                     IITRWPCAIKKFSQQDLEEFDLDRPHCRVIVEDCRGCKNCVRTGCPSISFDKEAKKAY
                     IDPTMCVGCTVCLQSCPFDAIERVGV"
     gene            complement(2809..3708)
                     /locus_tag="VFD08_05680"
     CDS             complement(2809..3708)
                     /locus_tag="VFD08_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZK10542.1"
                     /translation="MKHLEEIYSLAKGEPLNLAVVAAEDRAILQAVIQATEDKVINPI
                     LVGDQKKIEEILKELNCTKKMEIIPASTYEEGAKLVMKKASLGQVDFLMKGLLDTSII
                     LKALLTKEYGLRDRKVLSHMMLYEMKAYPKLIGLSDGGMNLSPSYEEKRAIFQNGIEV
                     FRALGYKEIKVAGLAAKEKVNEKMQATVDARRLQDEIKEEDVIVEGPLALDLIFSKEA
                     AEIKKFPSQIAGEVDFILVPTIETGNALGKSFSLIGGAKSAGLIVGAKLPLVLVSRAD
                     SFEAKLYSIALGKVFAKFLKEER"
     gene            complement(3710..4741)
                     /locus_tag="VFD08_05685"
     CDS             complement(3710..4741)
                     /locus_tag="VFD08_05685"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK10543.1"
                     /translation="MFKNVLEDLSFLQLRRDLRFGDLFLPKGLHLPLYTKQLEEKIKE
                     GEMDFGTEEILEGILSLYGADKDFKDKELYQPFIKKLQPLLPYLYEKRTNPKKALLMA
                     LGLIHLDLEEKGLYLFAAERCNDLIKEGLDCSELAMELLEKEKPSWPVGYHKGFLYYN
                     KERYHKALEAWGEILDKELPTEIRDEVFSMMALADKKKEYLGGRELLFKERFEEARQV
                     FKSLLADFPNWYELHFYYGLSLRLLEQNREALTVFYGLLQTKRDDIHLYNELAICHLF
                     LQEAEEARVLLEEGLKLEESSDLRLNLAISLFELGSKKEALEEIQKAKKLAPEDELID
                     HWLEHMRKE"
     gene            complement(4734..5573)
                     /locus_tag="VFD08_05690"
     CDS             complement(4734..5573)
                     /locus_tag="VFD08_05690"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZK10544.1"
                     /translation="MFKRIYIFHNRVKKSIAVKNQVMNLLRKEGYILTKQNPDLIVVI
                     GGDGTMLSAIRSMEKENIPFLGVNTGSLGFLPGILPDEIHLLPEILKEKNKNMDEFTL
                     LKVVSTTISGKKIVNYVFNEAVIKHYHPKLLEAHIYIDGKPFNYFTGDGLIFSTPVGT
                     TGYAVWAGGAAIHWDLPCYQITPLQPNDNRINRPLKNTIVIPAKSTVNLKVVKAKYRS
                     ASVACDGLRASKEYIRELTISTSDKKVRIMRGKKQSYFELYRNKIIDKNIFNYLEEEE
                     DSV"
     gene            complement(5566..>6043)
                     /locus_tag="VFD08_05695"
     CDS             complement(5566..>6043)
                     /locus_tag="VFD08_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HZK10545.1"
                     /translation="RKTHGLIASSFAPYEILATKELSFSELKLLKAIERMMDLYPKED
                     IPITLEVLSRNKGAFDVYYDIAKHFPKEQIYLRHSLENRLEILGNLYGDLIKETLELD
                     YSRKRRNRRFLFFPGRKSSKGKRILSYFDGKSLSKKETYYQIDYDKQLLYKEEEDV"
BASE COUNT         1681 a         1372 c         1060 g         1930 t
ORIGIN      
        1 ctaataacat ttcatgctcg tttattttag tataccatag ccaattattt gattttaagc
       61 agccacaaca tcttgttatt cagagaaata aaaaaacgat aatcaagtta tacttgctac
      121 cgctttttaa tggaggtggc acccggattt gaaccgggga atagaggttt tgcagacctc
      181 tgccttacca cttggctatg ccacctgttg gtcttctttt atggtgatcc atccgcgact
      241 cgaacgcggg actccctgat taaaagtcag gtgctctacc aactgagcta atggatcaaa
      301 tggtagcggc gagtggagtc gaaccaccga cctttcgggt atgaaccgaa cgctctaacc
      361 aactaagcta caccgccaca tttgttcagc tattatagaa tagcatcatc aggaaatact
      421 gtcaagagta tttcaagggt cccttggtgg gaccctatat actatactat gaattgtatc
      481 caaggtcaaa ggcttttagg ttgatttctc taaactcttc ctttacatat ttgttaacat
      541 agctaggcca atctatttct ttaagatcta gggccttggc taaggcccct aggagaataa
      601 tattttgaac cctaatatta cctaattcta tggccttatt tgaagcatca agaaagtgac
      661 agtcaaaacc ctttatgaga tgcttcttta ttccttctgg atatttggta tcacctgtta
      721 agattggggc tgaaggcagc ttataatcat tgataatggc ttttccacct ggcctaagat
      781 attcagacca ccttagagcc tccataagct caaaactcac aagaacatct gcttctccta
      841 aagagatcag cggcgagtca accttatctc caaagcgaag ctgggtcgtt acagagcctc
      901 ctctttgggc cattccaata acttcactca tcttaatatc atagcctgct tccataactg
      961 cagcaccaag aatcgtacta gccagaagaa tcccctggcc accaacacct gcaaatagga
     1021 tactcttttt catttataca cctactcttt caatggcatc aaagggacaa gactgtagac
     1081 ataccgtaca acccacacac atggtcggat caatataagc tttttttgct tccttatcaa
     1141 agctaataga agggcatccc gtccttacac agttcttaca tcccctacag tcttctacaa
     1201 tcaccctgca atgaggtctg tctagatcaa actcctctaa atcttgctgg gagaacttct
     1261 taatagcaca gggccaacgg gtaataatta cactaggacc ttgatcatac tctagtgctt
     1321 catttatagc actgcgggtc tcttcaagca taagaggatt aaccctaaga actttcttca
     1381 ctcctagagc cctaacaatc gcctctaagt ctacctgatt tgctggatct tcttggagag
     1441 tatatcctgt gccaggattt tgttgatgac ctgtcatggc ggttacccta ttatccaaga
     1501 ttacatttac ggtagatgaa ttattataaa ccgcattgag taaacttgtc attccagagt
     1561 gaagaaaggt cgagtctcca atcactccta ccaccttttt atcacttcca gttttttcca
     1621 gcgctttttt ggcgccatgg cccatagaaa gactcgctcc catacatata acagaatctg
     1681 ttgcattaag agggggtgct cctcctaggg tatagcaacc aatatctcct gtaataacta
     1741 catttcgttt ttttcctagt tcataaaaaa atccgcggtg gggacatcca gcacaaagaa
     1801 taggtggacg accaacaaga agttcgggat cagcctttat tgtttctgcc acttctccca
     1861 agagtttttc tttaataata tcaggattaa gttctcccat actaggaata agttctttgc
     1921 ctataacact caggccagct gctttaatct gctcctccat aaagggttcc agttcctcaa
     1981 caacatagac cttatcatgg gaagctataa agcgctcaat cttctttatg ggcaggggaa
     2041 aactaaaacc aatctttagt atatctgcag agtctccaaa tacttctttt acatagctat
     2101 agctgactcc tgctgtaata atacccaggg tgtttccata gtcctctagc acatttaagg
     2161 gactgctttc actatagtct gccagcttct gcagtctttc ttctaccata ggtcggcgta
     2221 ttctagcatt gcctggattt gctatgtatt tagaggcttg acgagtatag gcagttggct
     2281 ctacttcctt gcgaagtcct gtttcaacaa gacccttgct gtgacatacc ctggtagtca
     2341 tacgaatcag tactgggctg tcatagcttt cacttatttt ataggcctcc ctcataaaat
     2401 ctaaacactc ttggggcgtg gaaggttcaa gacaggcaat cttagcaaat ttagcataat
     2461 agcgattgtc ttgctcattt tgtgaagagt gacaacccgg atcatctgca ctaacaagta
     2521 aaaaacctcc ggttataccc gtatatccca tggtcataag aggatctgcc gcaacattga
     2581 cccccacatg tttcatggca gctagcgcgc gaactcctgc catagaggcc cctagagcag
     2641 actccagtgc taccttttca ttgggtgccc actctgcaac aatgtcctta tgatgggcca
     2701 agttttcaag aatttctgta ctaggcgtcc ccggataggc tgaagcgtag actactccag
     2761 cctcatatac ccctcgggca atggcctcat tacctgtcat aagttttttc atctttcctc
     2821 cttaagaaat tttgcaaaaa ccttaccaag ggcaatgcta taaagctttg cctcaaagct
     2881 atcagctcga ctaacaagaa caagaggaag ctttgctccc actataagac cagcagattt
     2941 tgctccccca ataagggaaa atgattttcc aagagcattt cccgtttcta ttgtaggaac
     3001 aagaataaaa tctacttctc ctgcaatctg agaaggaaac tttttgattt ctgctgcttc
     3061 tttactaaaa ataagatcta gggctagagg accttctact ataacatctt cctcttttat
     3121 ttcatcttgc aatcgccttg catccactgt agcctgcatc ttttcattaa ctttttcctt
     3181 tgcagcaagg ccggctacct taatttcttt gtatccaaga gccctaaata cttcgattcc
     3241 attttgaaaa atagctcttt tttcttcata acttggggat agattcatac ctccatcact
     3301 aagtccaatt agctttggat aggccttcat ctcataaagc atcatatggc taagtacttt
     3361 cctatctcta aggccatact ctttagtaag aagggccttt aatataatag atgtatcaag
     3421 aagtcctttc ataagaaaat caacttggcc caaagatgcc tttttcataa ccagcttggc
     3481 cccttcttca taagtagaag cagggatgat ttccatcttt tttgtgcaat ttagttcttt
     3541 taggatctct tctattttct tctgatcccc aacaagaata ggatttatca ccttatcctc
     3601 tgtggcctgt attactgcct gcaagatcgc cctatcttct gctgccacca ctgctaaatt
     3661 tagtggctcc cccttggcta gggaataaat ttcttctaaa tgcttcatat tactcctttc
     3721 tcatatgttc tagccaatga tctatcagtt catcctctgg agctagcttt tttgcctttt
     3781 gaatttcctc aagggcttct tttttacttc ccagctcaaa taaagaaatg gcgagattta
     3841 gtcttaggtc cgaactttct tccagtttta gcccttcttc taggagcact ctggcttcct
     3901 ctgcttcctg taaaaagaga tgacagatgg ctaattcatt ataaagatga atatcgtctc
     3961 tttttgtctg caaaagacca taaaagaccg ttagagcttc tctgttttgt tctagcaggc
     4021 gcaggctaag gccatagtaa aaatgcagtt cataccaatt tggaaagtca gcaagaaggc
     4081 tcttaaagac ctgccttgcc tcttcaaagc gttccttgaa aagaagttct cttcctccaa
     4141 gatactcttt ctttttatca gctagggcca tcatagaaaa aacctcatct cggatttctg
     4201 taggaagctc cttatcaagg atttctcccc aagcttctaa tgctttatga tagcgctctt
     4261 tattataata aagaaagccc ttatgataac caacaggcca agaaggtttt tctttttcca
     4321 gaagttccat ggccagttca gagcagtcaa ggccttcttt gattaaatca ttgcaccttt
     4381 ctgcagcaaa aagatacagc cctttttctt ctagatcaag atgaatcaaa ccaagagcca
     4441 ttagcagggc cttttttgga ttcgttcttt tttcatataa atagggaagt agaggttgga
     4501 gttttttaat aaagggttga tagagctcct tatccttaaa gtctttatct gccccataaa
     4561 ggcttaaaat gccctcaaga atttcctctg ttccaaagtc catctcccct tctttgattt
     4621 tttcttccaa ttgctttgta taaaggggca gatgtaagcc ctttggtaaa aaaagatctc
     4681 caaatctaag atctcttctt aactgtaaaa atgaaaggtc ctccagtaca tttttaaaca
     4741 ctatcttctt cctcctctaa ataattaaaa atattcttat caattatttt gtttcggtag
     4801 agctcaaaat aactttgctt tttaccccgc ataatccgta cctttttatc actagtagaa
     4861 attgtaagct ctctaatata ttccttagag gctctgagtc catcacaggc aactgaagca
     4921 gagcggtatt tggccttgac aacttttagg ttgacagtgg acttagcagg aataacaatc
     4981 gtgtttttaa gggggcggtt gatgcgattg tcattgggtt gcaagggtgt aatttgataa
     5041 caaggaagat cccagtgaat agcagctcct cctgcccaaa ctgcatagcc agtagtccca
     5101 acaggagttg aaaaaatcag tccatctcct gtaaagtaat taaaaggctt cccatctata
     5161 taaatatgag cttctagtaa ctttggatga taatgcttga ttaccgcttc attaaatacg
     5221 taattaacaa tttttttacc actaatagta gtactaacca ctttgagtag agtaaactca
     5281 tccatatttt tgtttttttc ttttagtatt tccggaagca gatgaatttc atcgggcaga
     5341 ataccaggaa gaaatcctag gctacccgta ttcaccccaa gaaaagggat attctctttt
     5401 tccatagaac ggatagcact aagcatggtg ccatcccccc cgattacaac tattaagtct
     5461 ggattttgtt ttgtcaaaat atacccttct tttcgaagga ggttcataac ttggttttta
     5521 acagcaatac ttttttttac tctattatga aagatataga ttcttttaaa catcctcttc
     5581 ctccttatat aggagctgtt tatcgtaatc aatttgataa taagtctctt ttttgcttag
     5641 actttttcca tcaaagtaac ttaaaattct ctttccctta gaagattttc ttcctgggaa
     5701 aaacaaaaag cgtcgatttc ttctctttct tgaataatca agttctagtg tttcttttat
     5761 caggtctccg tagagatttc ccagtatttc caatctgttt tctagtgagt ggcgaagata
     5821 gatttgctcc ttaggaaagt gcttggctat atcatagtag acatcaaaag cccctttatt
     5881 tcttgaaaga acttccagtg tgatagggat gtcttctttt ggataaagat ccatcatgcg
     5941 ctcaatggcc ttaagcagtt ttaactcaga aaaactaagc tccttggttg caagaatctc
     6001 gtagggggca aaggaagaag cgattaggcc atgggttttt ctt
//
LOCUS       DASRNA010000202         6496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_582952,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000202 DASRNA010000000
VERSION     DASRNA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582952"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..841
                     /locus_tag="VFD08_05700"
     CDS             65..841
                     /locus_tag="VFD08_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZK10546.1"
                     /translation="MRRKVAYFILGMGVLFLYLIKPRFKNKKKLRKFLKWNYAHRGLH
                     GGDRAENTLSAFKAAVNNGYAIEMDARSCKDRVVIHHDEDLKRSVGVDMRLDELDFAS
                     LGNYYLFGGKETIPSLEEALEVVDSKVPILLEIKSEKNHFKNASDVQAIMDKYEGPYL
                     IESFHPLVLLWYRLHRPEIIRGQLSAGNVEEGFFTSTAMALLLFNIISRPDFIAYEKK
                     GGLSPCLLYLLGTPSFVHTLRSHSERKWYFNVSIFEDYLP"
     gene            complement(868..2166)
                     /locus_tag="VFD08_05705"
     CDS             complement(868..2166)
                     /locus_tag="VFD08_05705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK10547.1"
                     /translation="MIIHSFGAAGQVTGSCHLVRSTQTFLFDCGLFQGTKEVDAQNTQ
                     DLPFDPSELDFVLLSHAHLDHCGRLPLLTQKGFSGPIYTSQASRDIVELMLRDSAKIQ
                     QMQKHPLYSEEDVDNTLSLLYPIEEGSKKTHGQTSFSFYPAGHFLGAHWIKVKTPEAT
                     IAFSGDLGRYDCPFYPQPYKLGRVDILLLEGTIGSRVHPPIKEGLNRLYKEVKKCIQK
                     KETVLIPAFSIGRTEEILSTLVKLALEEENHSFFDIPIYVDSKLARNGLTLFHKYDNL
                     HKMSLEHLAPDNLYLRDLKETASLKIEGPKILISASGMMEGGPIMVHAKKYLPDKETT
                     VFIVGYQGEETLGRQILTGQKEVTILEADLEVQAKIIEIKGLSGHGDSKDLQRFVSSA
                     QGLKTILLVHGEASSLQDLKEVFDKDYTVHIQKHNQPFEM"
     gene            2259..3149
                     /locus_tag="VFD08_05710"
     CDS             2259..3149
                     /locus_tag="VFD08_05710"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK10548.1"
                     /translation="MNKLEILEKRKSCRNYKNKELVAEDKRNIRSLLEAPHPLGNIPE
                     YELVFVDDGKRSAIKLEGFAGYSGVMIEAPHYIAVFSESDPANVRKTAYAVEDIILRM
                     IAMDIGSCWITVLDSYAAKIAMGWKNKGDLIALIALGYPQEDNYFSRLLSNLKHPLTN
                     PLKEGYGGLNFETKSGLPLREETSSFVYLDHWGNSTDHYELERRGLDRVYNYLKYAPS
                     WGNRQPWKFIVDHDDIVLCIHKNPKVSEIDENLDGGIAMYYFELAMTEHGLRGKWDFD
                     ALDKDYGIPQDYWTAGRFKL"
     gene            3158..4492
                     /locus_tag="VFD08_05715"
     CDS             3158..4492
                     /locus_tag="VFD08_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HZK10549.1"
                     /translation="MRRKLFFLLLLFFLVGCVSSQNFESVKKGTIGEEETQNYQIKKE
                     RIPKDYAYTQEDKVLLLSDDGEMIRRLNFQERVFVFRKKSKQSLVMDLYGNRGWVDQD
                     LLSKRFVSDIDLPLEGVNYNLYTVKDKPYDNYTKTKGIYIAPYGEEFIDETIERMKDT
                     PINTLVLDYHDDYGQVLFNSEVAAREVPEANVQLYEDKQGLIKKLKDKGYHLIARIVA
                     FKDPLYASSHQDKAIRHPDGSLLVSDGLYWNSPYDREVWEYLLDLSNEALELGFDEIQ
                     FDYIRFPDNFDSDMLAFNEQDETRAEAIQKFIMYIQANLSNKDAIISADVFGWVAIEM
                     ADVTIGQQWEAISNVVDVISPMFYPQLYGSGVFELADPQAHPYEVIKISMDYALRRNS
                     NVKTAAIIRPWLQAWNYSTEEINEQIKALDEAGIEEYLLWDVSGDYPKKGLN"
     gene            4492..5109
                     /gene="trmB"
                     /locus_tag="VFD08_05720"
     CDS             4492..5109
                     /gene="trmB"
                     /locus_tag="VFD08_05720"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZK10550.1"
                     /translation="MRVRKIKNLSQKMKDYQQLLIEDPQEIKRRFDKRPIELEIGFGK
                     GEFLLAMAKRHPEKNFLGIERSAEFVLKAMRRVGKLDNILFMTGNVQDLISQMDDDLF
                     SGLYLNFSDPWPKNRHFKRRLTYRVLLISYGRILKKGSIIQLKTDSRTLFDFSLEELT
                     ALGITPYDVTQDLHKDPSWSQNIATSYEIKFSSRGKKILGFRYRS"
     gene            5111..5584
                     /gene="lspA"
                     /locus_tag="VFD08_05725"
     CDS             5111..5584
                     /gene="lspA"
                     /locus_tag="VFD08_05725"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZK10551.1"
                     /translation="MIGIILGIIVLDRISKVLAVRFLQEASLALWENVFHLTYIENTG
                     AAFSLFKGHTLALGIFSLIMVALLSYFLYKRKKEGGGLLEQTALAFIIGGAVGNLYDR
                     LILGYVIDYFDFRLINFAVFNVADSFITVGAGLILLSIFLEEKKEKEKEKEKEGI"
     gene            complement(5971..>6496)
                     /locus_tag="VFD08_05730"
     CDS             complement(5971..>6496)
                     /locus_tag="VFD08_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZK10552.1"
                     /translation="DGGGYCRDKLIVLTTSTDGSIENNIKDASPEQQIKTFRFIAHEL
                     AHLWWLKAESTSWEDWLNESFAEYSSLMATRDFYGEKEFLRLINLYKEKTLNLPAVRG
                     IDRSDQDAFNVLYAKGPTLLYQLEIMIGAVAFKELLNNIHMKKINNTLDFLDELTLLT
                     SKDTSKKFSKLLDL"
BASE COUNT         1998 a         1129 c         1403 g         1966 t
ORIGIN      
        1 gagcagacac tgctaaagtg aatgaagaaa attacttatc gatttataag gaaaatttga
       61 gaaaatgaga agaaaagtag cttattttat cttaggtatg ggggtattgt ttttatactt
      121 gattaaacca agatttaaaa acaagaaaaa actccgaaag tttttaaagt ggaactatgc
      181 tcacagggga cttcatggag gtgatagggc agaaaacacc ttaagtgctt ttaaggctgc
      241 ggtgaataat ggatatgcca tcgaaatgga tgcaagaagc tgtaaagaca gggtagtaat
      301 tcatcatgat gaagatttaa agcgttcagt tggagtagat atgaggctag atgaacttga
      361 ttttgcaagt ttaggaaatt attatctttt tgggggtaag gaaacaattc catccctgga
      421 agaagcactt gaagttgttg actcaaaggt tcccattttg ttagaaataa agagtgaaaa
      481 aaatcacttt aaaaatgcca gtgacgtcca ggctattatg gataagtatg agggaccata
      541 cctgatagag agttttcatc ccttggttct tttatggtat agactgcata ggccagaaat
      601 tataagaggt cagttgtctg ctggaaatgt ggaagaaggg tttttcactt ctacggctat
      661 ggcacttttg ctctttaata ttattagtcg tccggatttt attgcctatg aaaaaaaagg
      721 tggtttatcg ccttgtcttc tttatctgct agggacacct agttttgtcc atacccttcg
      781 gtcccacagt gaaagaaaat ggtatttcaa tgtaagtata tttgaagatt atcttcctta
      841 aaaaaaagag agggctgccc tctcttttta catttcaaaa ggttgattgt gtttttgaat
      901 atggacagtg tagtccttat caaacacttc ttttaggtcc tggagggaag aggcttcccc
      961 atgtaccagg agaatggttt taagcccctg tgcagaggaa acaaagcgct ggaggtcttt
     1021 actatccccg tggcctgaaa gtccctttat ttcgataatc ttagcttgaa cttctaaatc
     1081 tgcctcaagt attgtaactt ctttttggcc tgtaaggatt tgtctaccta gggtttcttc
     1141 tccctgataa ccgacgataa aaacagtggt ctctttatca ggcaggtact ttttggcatg
     1201 aaccataatg ggaccaccct ccatcatgcc tgaagctgaa attagaatct ttggcccttc
     1261 tatttttaat gaagccgttt cttttaaatc acgaagatat aaattatcgg gcgcaagatg
     1321 ttcaagggac attttgtgta aattgtcata tttatgaaat aaagtgaggc catttcgcgc
     1381 aagctttgaa tccacataaa tggggatgtc aaaaaaagag tggttttcct cttcaagagc
     1441 aagttttaca agagtggaaa gaatttcttc ggtcctgcca atagaaaagg cgggaattag
     1501 aaccgtttcc tttttctgga tgcatttttt cacttccttg taaaggcgat ttaagccctc
     1561 tttaatagga gggtgaaccc ttgatccgat tgtgccctca agtaggagga tatcgaccct
     1621 accgagctta taaggctggg gatagaaggg acagtcatat ctaccaaggt ctccgctaaa
     1681 agcgatggtg gcttcaggag tttttacctt aatccaatgg gcgcctaaaa aatgtccagc
     1741 aggataaaaa gagaaggacg tttgcccatg ggttttctta ctgccttctt caatgggata
     1801 aagaagagaa agggtattgt caacatcttc ctcactataa agggggtgtt tttgcatttg
     1861 ttgaattttg gcgctgtctc ttagcataag ttcgacaata tctctgcttg cctggcttgt
     1921 atagattggc cctgaaaatc ctttttgtgt taaaagaggg agccgtccac agtggtctag
     1981 gtgggcatga ctaaggagga caaaatcaag ttcagaggga tcaaagggca aatcctgtgt
     2041 attttgcgca tcgacctcct ttgttccttg aaagagtccg cagtcaaaca aaaaagtttg
     2101 ggtggaccta accaagtggc aagaacctgt tacttggcca gcggcaccga aagaatgtat
     2161 tatcatagtt acctcctttt aatcttacta cccacttcca aagaggagct cttgtaattc
     2221 tttgaaaact cctctataat ttactcacgg aggtggatat gaataagtta gaaattcttg
     2281 aaaaacgcaa atcatgtcgc aattataaaa acaaagagct tgtggctgag gataaaagaa
     2341 atatacgatc tcttttggaa gcacctcatc ctttaggaaa cattcctgag tatgagttgg
     2401 tttttgttga tgatggtaag cgcagcgcaa ttaaactaga aggctttgct ggttatagtg
     2461 gtgttatgat agaggctcct cattatattg cagtcttttc tgagagcgat ccagctaatg
     2521 ttcgcaagac agcctatgca gtagaagata ttattttacg catgattgct atggatattg
     2581 gtagttgctg gattactgta cttgattcct atgcagcaaa gattgctatg ggatggaaaa
     2641 acaagggaga tctgattgct ttgattgcac taggatatcc acaggaagat aattatttta
     2701 gtagactttt gtcaaatttg aagcatccct tgaccaatcc tttaaaggag ggctatggtg
     2761 ggcttaattt tgagaccaag agcggtttac cccttcgaga agaaacgagc agttttgtct
     2821 atctagatca ttggggcaat agtacagacc actatgaact agagcgtcga ggcttggacc
     2881 gagtctataa ttatctaaaa tatgcaccaa gctggggcaa tagacagccc tggaaattta
     2941 tcgttgacca tgatgatata gtactatgta ttcataagaa tccaaaggtg agcgaaattg
     3001 atgaaaacct tgatggagga attgccatgt actattttga actggcaatg acagaacatg
     3061 ggcttagggg aaaatgggat tttgatgctt tggataaaga ctatggtatc ccccaagact
     3121 attggacagc aggaaggttt aaactataag gggttttatg cggcgaaaac tattttttct
     3181 tctgctccta tttttcttag tgggctgtgt tagctcacaa aactttgaaa gtgttaaaaa
     3241 gggaacgatt ggggaagaag aaacacagaa ctaccaaatt aaaaaagaaa gaattccaaa
     3301 ggactatgcc tatacccagg aagacaaggt gcttctttta agtgatgatg gcgaaatgat
     3361 tcgcagatta aactttcaag agcgcgtctt tgtgttcaga aaaaagtcta aacaaagcct
     3421 ggtgatggac ctatatggca atcggggctg ggtggaccag gacctattat ccaaacgctt
     3481 tgtcagtgat atagatctgc ctctagaggg ggtaaattac aatctatata ctgttaagga
     3541 taaaccctat gataattata ccaaaacaaa gggcatctac atagcccctt atggagaaga
     3601 gtttattgat gagacaattg agcgtatgaa agacactccc ataaataccc ttgttttaga
     3661 ttatcatgat gactatggac aagttctttt taatagtgaa gtagctgcaa gagaagtacc
     3721 agaggctaat gttcagctct atgaggacaa gcaaggactg attaagaaac taaaagacaa
     3781 gggctatcat cttattgcgc gcatagtagc ctttaaagac cctctttatg ccagtagtca
     3841 tcaagacaag gccatacggc atccagacgg gagtcttcta gtttccgatg gtctttactg
     3901 gaattctccc tatgatcggg aggtgtggga atatctacta gacctaagta atgaagccct
     3961 ggaattaggt tttgacgaaa ttcagtttga ctatattcgt tttcctgata attttgattc
     4021 ggatatgcta gcttttaatg agcaagatga gactagggca gaagccatac aaaagtttat
     4081 tatgtatatt caagccaatc tcagtaataa agatgccatt ataagtgcgg atgtttttgg
     4141 ttgggttgca atagaaatgg cagatgttac cataggccag cagtgggaag ccataagcaa
     4201 tgtggtagat gtaatcagtc ccatgtttta tcctcagctt tatggctctg gagtttttga
     4261 gctagctgac ccccaagccc atccctatga ggtgattaag atatcaatgg attatgccct
     4321 taggcgtaat agcaatgtaa aaacggcggc tattatccgc ccttggcttc aagcttggaa
     4381 ttattcaaca gaagaaataa atgagcaaat taaagccctt gatgaagcag gtattgaaga
     4441 atatcttctt tgggatgtaa gtggcgatta tcctaagaag ggactaaatt gatgcgggta
     4501 cgcaaaataa aaaacttatc gcaaaaaatg aaagactatc agcagctact aattgaagac
     4561 ccccaagaaa taaaaagacg gtttgataaa agacccatag aacttgagat tggctttgga
     4621 aaaggggaat ttcttcttgc catggcaaaa agacatccag aaaaaaactt tcttgggatt
     4681 gagcgcagtg ctgaatttgt attaaaggca atgcgccggg taggaaaact agacaatata
     4741 ctctttatga caggaaatgt tcaggacctt ataagccaaa tggatgatga tttatttagt
     4801 ggtctttatc ttaacttttc ggatccctgg cctaaaaaca ggcattttaa gcgccgattg
     4861 acctacaggg ttcttcttat aagctatgga cgcatcctaa aaaaagggtc tatcatccag
     4921 cttaaaaccg acagccgcac cctttttgac tttagtcttg aggagctgac ggccctgggt
     4981 ataaccccct atgatgtgac acaagacctt cacaaagacc ccagttggag ccaaaacatt
     5041 gccactagct atgagataaa attctcttcc cgggggaaaa agatcctcgg ttttagatac
     5101 aggagctagt atgattggaa taattttggg gattattgtc cttgatagga ttagtaaggt
     5161 tttagctgtt cgctttcttc aggaagcctc ccttgcactt tgggaaaatg tttttcacct
     5221 tacttatatt gaaaatacag gagctgcctt tagcttattt aagggtcata ccctggccct
     5281 gggcattttc tctcttatta tggtggcact tctttcttat tttctttata aaagaaaaaa
     5341 agaaggtggg ggacttttgg agcaaacagc tctggctttt attattggag gggcagttgg
     5401 aaacctttac gatcgcctta tcctgggtta tgtgattgat tattttgatt ttaggctgat
     5461 taattttgcg gtctttaatg tagccgatag ttttattact gttggggcag gactaattct
     5521 tttgtcaatt tttcttgaag aaaaaaagga aaaagaaaaa gaaaaagaaa aagaaggcat
     5581 ataatagctc ctagaattta aaatgacccc aaggttggac ctagaatcca atgaagaggg
     5641 tcattttaat gtcaaaatat tcactggaaa gaaaagggaa atgttgagcc aggctcaacg
     5701 aaaaaggagg atataaaaca ctcactagaa ggtgtgtacg gtaatctaaa agaaaaaagt
     5761 aactagccag tgcaatccaa acataatacc ctaatagtag cggggagccc tagctttttc
     5821 gtagtcctag aagcctcttt ataggatgcg atgtctgcct attttctcgg aaatatgcag
     5881 acttactcag gcccctaggc tttgtggcta tcattaaaga tcttttaaat ggctccttca
     5941 attaagattt tccaaagtct aaagtttttc ctatagatct aaaagttttg aaaacttctt
     6001 agaagtgtct ttacttgtta atagggtcag ttcatcaaga aaatcaaggg tattgtttat
     6061 ttttttcata tgtatattat ttagtaattc tttaaaagct actgctccta tcatgatttc
     6121 taattgatac agtaaagtcg gtccttttgc atataaaaca ttaaatgcat cttgatcact
     6181 tctatcgatc cctcttacag ctggtaagtt tagggttttt tctttgtata aattaattaa
     6241 tcgcaaaaat tctttttcac cataaaaatc tcttgttgcc attaaagatg aatactcagc
     6301 aaaggattca ttcaaccaat cttcccagga agtagattct gctttaagcc accataaatg
     6361 ggctaattca tgggcaataa atctgaaggt ttttatttgc tgctcaggac ttgcgtcttt
     6421 tatattattt tcaatactac catcggttga tgttgtaaga acaataagct tatctctaca
     6481 gtagcctcca ccatca
//
LOCUS       DASRNA010000203         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_737179,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000203 DASRNA010000000
VERSION     DASRNA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737179"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFD08_05735"
     CDS             <1..686
                     /locus_tag="VFD08_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HZK10553.1"
                     /translation="FQDPFASLNPRRTISETIMEPMIIHNMYDNNKTRMEKVLEIMEI
                     VGLAERLVNSYPHELDGGRRQRIGIARALALEPKFIVCDEPVSALDVSIQAQILNLLK
                     DLQVRLGLTYMFITHDLSVVNHFSDDIAVMYLGKFVERASSDDIFLKPLHPYTQALLS
                     AIPVPDIHYKSERILLEGELTSPIEPEKKCRFANRCAHAFDRCFKEEPEFLEVEPNHW
                     VACFLHDKK"
     gene            756..1907
                     /locus_tag="VFD08_05740"
     CDS             756..1907
                     /locus_tag="VFD08_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK10554.1"
                     /translation="MKAMVNVKIHTGTGKTLEKGTVLFSDKILKVGENISLPEGVEVI
                     DGEGLVLTPGLIDPHTHICNFQGRSTLPGVKDGNEYSSPVTPYVRALDAVNPEDVAIA
                     RTRRAGFTTVFTTPGSANVIGGVGCAIKLRGMTAEDMYIPGTEMMKMALGENPKRFFG
                     LQKKLPVTRMGVAGLLRQTLYDAKVYSDALLAYEKGEGEKPTADFEKDALVSVVRGEQ
                     VCRIHAHRADDIVTANRIAKEFGLDFVVEHATEGYKIKEYLAKEKVRCVVGPVALSPA
                     KLELWDISLNNAKELSQAGVTIALTEDTDYNTWQLLMHIGILIREGLSWEVALGGITL
                     EAAKILKLDHRVGSVEEGKDADFAIFDGDLFSNYTNCLMTIIDGVIYTD"
     gene            1961..>2156
                     /locus_tag="VFD08_05745"
     CDS             1961..>2156
                     /locus_tag="VFD08_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZK10555.1"
                     /translation="MKTVSDVFNEICDFEKRVGLFYESIGDEVKKEYGKFFENLAEDE
                     YRHAEIYKKLGEKAARDERVI"
BASE COUNT          675 a          381 c          485 g          615 t
ORIGIN      
        1 tctttcagga cccctttgcc tctttaaatc caagaagaac gataagtgaa accatcatgg
       61 agcccatgat tattcacaat atgtatgata ataataaaac acgcatggaa aaagtgctag
      121 aaattatgga aattgttgga ctggcagaaa gattggttaa ctcctatcct catgaactag
      181 atggcggaag acgtcaacgt attggtattg ctagagctct ggctcttgaa ccaaagttta
      241 ttgtttgtga tgaacctgtt tcagcccttg atgtttccat ccaagcccag attcttaatc
      301 tactcaagga tcttcaggtg cgtcttggtt taacctatat gtttattacc catgaccttt
      361 ctgttgttaa tcacttttct gatgatatcg ctgttatgta tcttggaaag tttgtagaaa
      421 gagcaagttc agatgatatt ttcttaaagc ctctacatcc atatacacaa gctttattat
      481 cggccatccc ggttcctgat attcactaca aaagtgaaag gatcctgcta gaaggtgaat
      541 taacatcacc cattgagcca gagaaaaagt gtcgttttgc caatcgctgc gctcatgcct
      601 ttgaccgatg ctttaaggaa gaaccagaat tcttggaagt tgaacccaac cattgggtag
      661 cctgcttcct acatgataaa aaataggggt aatccccagt aagaagctag ggtaactcct
      721 agctttttac atcttaaata tagatggagg caaagatgaa agcaatggta aatgtaaaaa
      781 tccacactgg aacaggaaaa acactggaaa aagggaccgt tcttttttca gataaaattc
      841 taaaagtggg tgagaatata agtcttccag aaggggtgga agtaattgat ggtgagggac
      901 ttgtattaac accgggcctg attgaccccc atactcatat atgcaacttc caaggaagaa
      961 gtacccttcc tggtgtaaaa gatggaaatg agtattcttc tccagttact ccctatgtga
     1021 gagcccttga tgcggtaaat ccagaagatg ttgcaatagc aagaacaaga agggctggtt
     1081 ttactacagt atttacaaca ccaggctctg ctaatgtaat cggtggagtc ggttgcgcga
     1141 ttaaacttag ggggatgaca gccgaagata tgtatattcc aggcactgag atgatgaaaa
     1201 tggccctggg agaaaaccca aagcgttttt ttggtcttca aaaaaagctg cctgtaaccc
     1261 gtatgggtgt tgctggactt ctgcgccaga ccttgtatga tgccaaagta tatagtgatg
     1321 ctcttcttgc ttatgaaaaa ggcgaaggtg aaaaacctac agctgacttt gaaaaagatg
     1381 ctttagttag tgttgttcgt ggcgagcaag tttgtcgcat tcatgcccac agggccgatg
     1441 atattgtaac ggcaaatcgc atagcaaaag aatttggttt ggactttgtt gtagaacatg
     1501 ccactgaagg ctacaaaatt aaagagtatt tagccaagga aaaagtccgc tgtgttgttg
     1561 gtcccgtggc actttcacca gccaagctgg aactgtggga tatttcactt aataatgcca
     1621 aggaacttag ccaggcggga gttaccatag cccttacaga agataccgat tacaatactt
     1681 ggcaacttct tatgcatatt gggattttga ttcgggaagg actttcctgg gaggtggctc
     1741 ttggtgggat tacactagaa gcagccaaga tcttaaagct tgatcataga gtgggtagcg
     1801 ttgaagaggg aaaagatgct gattttgcta tatttgatgg cgatcttttt tccaattaca
     1861 ccaactgcct tatgacaatt attgatgggg ttatctatac cgattaataa gtctatgtta
     1921 taatgatatg taacaataaa aagacataag gaggttattt atgaaaacag tatcagatgt
     1981 ttttaacgaa atttgtgact ttgaaaaaag agtaggcctt ttctatgaga gcattggcga
     2041 tgaagtgaaa aaagagtatg gtaaattctt tgaaaattta gctgaagatg agtaccgaca
     2101 tgcggaaatt tataaaaaac taggtgaaaa agcagctaga gatgaaagag tcatac
//
LOCUS       DASRNA010000204         7023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_504807,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000204 DASRNA010000000
VERSION     DASRNA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504807"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VFD08_05750"
     CDS             <1..1740
                     /locus_tag="VFD08_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HZK10556.1"
                     /translation="SFYLLTGAGQIARNGDEIIRLIVNNAKWVFLLALPVILYFFFFR
                     KSLSQLGDKKTEGIKLCGLASLFFLVLHYSMGAFPPQEKRISDLYFYTNPLVETTREF
                     GLVSSLGIDLRQRIFGEPIPKELIKEEKYEDKYIEEGKAEEIFYHENIPLEKELVQEE
                     IVIEPQILDIDFKALEKQRESETLALLDGYFAFLEPSYSNEYTGIFEGYNLIFITAES
                     FWWPAVDQELTPTLYQMIHQGIHLENFYNPIWTVSTYDGEYVGLTGLLPKDGVWSLYR
                     AAFNFSPQSLGWRMDAANYNCYAYHNHTYTYYDRHESHPNLGYDYKGLGSGVDVQVQW
                     PGSDLEMMEITMDEFLDKEPFHVYYLTVSGHGAYSFSGNAMANKNKAYVEELDLSEEA
                     ASYIAANMELEHALSYLLARLEEEGLAERTLIVLNPDHIPYSLSEEAMEELAGSFSRE
                     IDRYRSHGIIYTPNMEGIEIKKPVSSLDLLPTVYNLLGLSYESRLIIGRDVFSEQGLV
                     IFYNRSWLSEKGYYNSRSEKFESMVEEEIPEGYVDKINQEVKEKFYFSGLLLEEDYFS
                     YLPEKAFSFQKKE"
     gene            1837..2247
                     /locus_tag="VFD08_05755"
     CDS             1837..2247
                     /locus_tag="VFD08_05755"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZK10557.1"
                     /translation="MQRGFILILILAVIIGIFVISNSGVVAIDFIFTEVMISQAIVIF
                     ICVLLGAILATIFGGIRQMSLKKEIKQGRTEISRLESENEKVKRELDLLQSENQKLKK
                     DIYRPSAQGKEEEGSFIDVISRSHEKTPKQTKDL"
     gene            2654..3688
                     /locus_tag="VFD08_05760"
     CDS             2654..3688
                     /locus_tag="VFD08_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZK10558.1"
                     /translation="MGLDIEGLPQTSSLDRRATFEGWYRNEEGSFFYKKMKKQVGWQE
                     IEDSPYHFNEQGLMTRGWLEEDHHYYLCPESGARAQGWTMIDDKHYYFHPKKGEMIQS
                     AWITEGDKRYYIKSSGNPAKDLYTVDGVDYYFDERTGECLLDASKPMIALTFDDGPVE
                     NTEELLDFLDENSVDVSFFLQGKSIPGKEDLVKRMQEEGHTVGNHSYNHPKFTELSEE
                     EILWQVEETDRLIYEATGSKPIFFRSPYGLTDKRIESVLARPTILWSVDSEDWNGRNA
                     DEIHHHLIDKAKDGDIVLMHDTYSTTIKAIKKTIPLLREREFQFVNMETLFKVKGQKP
                     QGRDVYYYLE"
     gene            3809..4594
                     /locus_tag="VFD08_05765"
     CDS             3809..4594
                     /locus_tag="VFD08_05765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HZK10559.1"
                     /translation="MNKEIKLVALDIDGTLLDSKGKISSRTRKKIKSLIEKDKYVALA
                     SGRASKAAANIKEELSLDLPIISYNGSALHLQGGEKVLDRKIPLEDALEVIEFAENKG
                     IFVKAYIDDLFYIEKDHKLIKAFSQYQNIGYRVVGKLSEYIKEDVNMIVFIYDELSSV
                     DLDMLFKDLAVTTARSTPFVVEFMAKGATKAAALKLLASHLGIKKEEILAVGNALNDL
                     EMIEYAGTGFAMKNSDPDLLKKWDRVSDYSNDEEGVYHIIKDL"
     gene            complement(4650..5618)
                     /locus_tag="VFD08_05770"
     CDS             complement(4650..5618)
                     /locus_tag="VFD08_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZK10560.1"
                     /translation="MKKILMGKIADKILKLNLNMKKDEKLLIVTEPEKMSIAETIAAS
                     ALRMDIEPIIAIMMPRHTDGQEPPKIIAQAMSASDAFLSIVGKSITHTNAVKEAIEKG
                     SRGLVLTQFSEDMMIEGGMEADFVSLAPLCKDVARALESANEIQLTSPFGTDLTFSAK
                     GRRGNALYCMVEKGQFSTAPTIEANVSPIEGTAKGRIVADASIPYLGIGLLEEPMYFD
                     VEDGNIVSIQGGRQADILREDLASKNDPGVYNIAELGIGLNPNCRFIGLMLEDEGVFG
                     SVHIGIGTSVNLGGKVKAASHYDLIMTRASLFLDGKLILKDGELLL"
     gene            6135..7004
                     /locus_tag="VFD08_05775"
     CDS             6135..7004
                     /locus_tag="VFD08_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZK10561.1"
                     /translation="MKRIVVLLLAFVLILSLGACAGKEETPEAVAPEAEEQTEKGDLA
                     DGIYFAMEDEFADSGWKSTVTLKVEGGEIVSADWNAVNILGGVDKKTFSKDGDYGMKE
                     FGNAQSHWHEQAQEVEAYLVQTQDPTAIEYKDDEGHTDAIAGVSVHVNDFFELAKKAL
                     DKEPIELGQYEDGAYHAQAKEFVNGWKDTVDLTVLNGHILSVSWDGISEEDPEITKKT
                     ASADGSYGMKEEGNAQSHWHEQAQKAEAYLLETQDPRKIDYSDDQGHTDAISGVSIGV
                     NGFFDLAKEALEK"
BASE COUNT         2365 a         1143 c         1491 g         2024 t
ORIGIN      
        1 agcttttatc tacttaccgg ggccggacaa atagctagga atggcgacga aattataagg
       61 cttatcgtta ataatgccaa atgggtcttt cttttagccc tgccagttat cttatatttt
      121 ttcttcttta gaaaaagtct tagccaactt ggggacaaaa aaacagaagg gataaaactt
      181 tgtggactag ctagcttgtt ttttctcgtc ttgcattata gtatgggtgc ttttcctccc
      241 caggaaaaaa gaataagcga cttatatttt tacaccaatc ctttggttga aaccactaga
      301 gaattcggcc tagttagttc cctgggcatt gatctaagac aaaggatttt tggtgaacca
      361 atacccaaag aactgataaa agaagagaag tatgaagata agtatataga agaaggtaaa
      421 gcagaagaaa tcttttatca tgaaaatata ccccttgaaa aagagctagt tcaagaggag
      481 attgtaatag agccccagat tttggatata gattttaagg cactagaaaa gcaaagagaa
      541 agtgaaacgc ttgctctttt agatgggtat tttgcttttc tagagccttc ttatagcaat
      601 gagtatacgg gtatttttga gggctataat ctaattttta tcacagcaga atccttttgg
      661 tggccagcag tagatcagga gctaaccccc accctttatc aaatgatcca ccagggaatt
      721 catttggaaa acttttacaa cccaatttgg acagtttcaa cctatgatgg agagtatgtt
      781 ggtttaacgg ggctactacc taaggatggc gtttggagtc tttatagagc ggcctttaat
      841 ttttctcctc aatctctagg ctggaggatg gatgcagcaa attataattg ctatgcctat
      901 cataatcaca cctataccta ttatgatcgc cacgaatctc atcctaatct tgggtatgac
      961 tacaagggac taggttcagg agtagatgtg caggtccagt ggcctggttc tgatcttgag
     1021 atgatggaaa tcaccatgga tgagttttta gataaggaac cttttcatgt atattatctt
     1081 actgttagcg gccatggagc ctatagtttt tcgggaaatg caatggcaaa taaaaacaag
     1141 gcctatgtag aagaactgga tttaagtgaa gaagcagcca gttatattgc cgctaatatg
     1201 gaactggaac atgctctaag ctatcttctt gccagactcg aagaagaggg tctggcagag
     1261 agaacactta ttgttcttaa tccagatcat attccctatt ctttgagtga agaggctatg
     1321 gaagaattag cagggagttt ttctcgtgaa attgatcgtt accggagtca tggaattatt
     1381 tatactccaa atatggaggg gatagagatt aaaaaacccg tttcgagttt ggatcttcta
     1441 cccacggtat acaatcttct aggcctaagt tatgagtccc gcctgattat aggtcgagat
     1501 gtcttttccg aacaaggatt ggttattttt tataatcgga gctggcttag cgaaaaaggc
     1561 tactataact cacgaagcga aaagtttgaa tccatggtag aagaggaaat accagaagga
     1621 tatgtagata aaataaatca agaggtaaag gaaaagttct attttagtgg tcttttatta
     1681 gaggaagatt atttttctta ccttccagaa aaagcatttt cattccaaaa aaaagaataa
     1741 gcgctagttt tacttctttt ctagcaggaa aagagtagag agaatactta aaactaaaga
     1801 gaaaagatat aagaaaaaaa taaaaggagg actattgtgc aaaggggatt tatacttata
     1861 cttatactgg ctgtaatcat tggtatattt gttataagta atagtggggt tgttgcgatt
     1921 gattttattt ttaccgaagt aatgatatct caagccatag tgatttttat ttgtgtgctc
     1981 ttaggggcga ttttagcaac aatatttggt gggataagac aaatgtccct taaaaaagag
     2041 attaagcagg gaagaacaga aataagcaga cttgaatcag aaaatgaaaa agtaaaaagg
     2101 gaattagacc tgcttcaatc agaaaatcag aagctaaaaa aggacatata tagaccaagc
     2161 gcgcaaggaa aagaggaaga gggtagtttt atcgatgtta ttagtagaag tcatgaaaaa
     2221 actcccaagc aaacaaagga cctctaattg ctcctatagc aaataataag gaaaaagaaa
     2281 cttcaatcgg ctgattgagg tttcttctag ctacttggac tagtgaaagc attctcaact
     2341 tatagcttaa gctggtccag tagttttgaa aatagaaaaa actagccggc aggcctgtgg
     2401 acttatttca aagtattagc aatgcctttt gcctacaaaa ggtaagttta caggccatag
     2461 cagcaagtaa tatacttaaa ggcaaaaggt ttcctttaaa agaagctagt gctttttctt
     2521 accaaaaagc ctagaaaact ataaggaaaa agctgacaag gaggatgaaa tcaaaaagaa
     2581 aaactctcta tcatttattt ttataacagt acttataagc tgtggacttc tcttttttgt
     2641 tcagtataga gtaatgggcc tagatataga agggctgccc cagacttcaa gccttgatcg
     2701 aagggccact tttgaaggct ggtataggaa tgaggaaggc agcttttttt ataaaaaaat
     2761 gaaaaaacaa gttgggtggc aagaaataga agattccccc tatcatttta atgagcaagg
     2821 acttatgaca aggggttggt tagaagaaga tcaccactac tatctttgcc cagaaagtgg
     2881 agcaagagcc caaggctgga caatgattga tgataagcac tactacttcc atcctaaaaa
     2941 aggagaaatg attcaatctg cttggattac agagggggat aagcggtatt atataaaatc
     3001 cagtggaaat ccagccaagg atttgtatac tgttgatggg gttgattatt attttgatga
     3061 aagaacaggg gaatgtttgc tggatgcttc aaaacccatg attgctttaa cctttgatga
     3121 tggcccggta gaaaacacag aggaattatt agattttttg gatgaaaact cagtagatgt
     3181 gtcttttttt ctgcaaggaa aaagcattcc aggaaaggaa gacctggtaa agagaatgca
     3241 agaagagggc catacagttg gtaaccattc ttataatcac cccaaattta ctgagcttag
     3301 cgaggaagaa attctttggc aagtcgaaga aacagatcgt cttatttatg aggctacagg
     3361 tagtaaacct attttcttcc gctctcccta cggccttacc gacaaacgga tagagagtgt
     3421 ccttgccagg ccgaccattc tttggagcgt ggactcagag gactggaatg gtagaaatgc
     3481 tgatgaaatt caccatcatc ttatagataa ggcaaaagat ggtgatattg ttcttatgca
     3541 cgacacctat agtacaacca ttaaggcgat taaaaaaaca attccccttc ttagggaaag
     3601 ggaatttcag tttgtaaata tggagactct ttttaaagtc aaaggacaaa aaccccaagg
     3661 gagagatgtt tattattatc tagagtaaag agcttagaat cagaagagtt atggtcttag
     3721 gattcaaggg ctataggtca aaacacaaag aagacaccag cttagatagg cagatggtga
     3781 aaaaaataga aatggagtag tttacctttt gaataaagaa attaaattgg ttgcattaga
     3841 tattgatgga actttactag atagtaaagg caagatttct tccagaacaa ggaaaaaaat
     3901 aaaaagtctt atagaaaaag ataaatatgt ggcactagct tcaggacgag ccagtaaggc
     3961 cgctgctaat ataaaagagg aattaagctt agatttacct ataatctcat ataatgggag
     4021 cgccctacac ttgcaggggg gagagaaggt gttagatcgt aagattcctc tagaggatgc
     4081 ccttgaggtt atagaatttg cagaaaataa gggcatattt gttaaggcct atattgatga
     4141 tttgttttat atagaaaaag accataaatt gataaaagcc ttttcccaat atcaaaatat
     4201 aggttacagg gtagtgggca agttaagcga gtatattaaa gaagatgtca atatgattgt
     4261 ctttatatat gatgagctat ctagcgttga tcttgatatg ctctttaaag atttagcggt
     4321 taccacagca agatcaactc cctttgttgt tgagtttatg gctaagggag caacaaaggc
     4381 agcagcacta aaacttttgg ctagtcattt agggataaaa aaagaagaaa tacttgctgt
     4441 aggaaatgca ttaaatgatt tagaaatgat agagtatgct gggacagggt ttgcaatgaa
     4501 aaactccgat cctgatttat taaaaaaatg ggatcgtgtt tcagactact ccaatgatga
     4561 agaaggtgtt tatcatatta ttaaagactt ataggaaaaa agacagattg caaagtctgt
     4621 ctttttttct gagaaagtag aggaagaggt taaagaagaa gctccccatc ttttagtatt
     4681 agtttaccat ctagaaaaag gcttgccctt gtcataatca aatcataatg actggccgct
     4741 tttaccttgc cgccaagatt tacactggtt ccaattccaa tatgaacaga accaaagact
     4801 ccttcatctt ctaacataag gccaataaat cgacaatttg gatttagccc gatgcccagt
     4861 tctgcaatat tgtagacccc ggggtcattt ttacttgcta gatcttctcg taagatatct
     4921 gcttggcgtc caccttgaat agagacaata ttgccatctt ccacatcaaa atacataggt
     4981 tcttctagca agccaattcc aagataggga atactagcat cggctacaat tcttcccttt
     5041 gccgtacctt ctatgggaga tacatttgcc tctatcgttg gtgcggtaga aaactgacct
     5101 ttttctacca tgcagtaaag ggcatttccc cttcttccct tggctgaaaa agtaaggtct
     5161 gttccaaagg ggctagtgag ttgtatttca ttagcagact ctaaggctct agccacatcc
     5221 ttacagaggg gggccagtga gacaaaatct gcttccatcc caccttctat catcatatct
     5281 tcgctaaact gagtaagaac caagcctcta gacccttttt caatggcttc ttttacggca
     5341 ttggtgtgag tgattgattt tcccacaatg gataaaaaag cgtcactggc actcattgcc
     5401 tgggctatta ttttaggtgg ttcttgtcca tcggtatgtc ttggcatcat aatagctata
     5461 ataggttcta tatccattct aagagcagag gcagcaatgg tctctgctat agacattttt
     5521 tcaggctcgg ttacaataag taatttttcg tcctttttca tatttaggtt tagttttagt
     5581 attttatctg cgattttccc cattagtatt tttttcaatc ctatagcctc ctagtttggc
     5641 aaatttaatt aatagtacaa tctaaggtat caattcctat tttagtatag cagtccttgg
     5701 acataatggt catcagaaaa gaagataaac tataatcttt tgggtataag gctcataaga
     5761 aaaggtaata attttgtgat gcttagagcc aggttagagt gaaaaactca aatgtgaaca
     5821 tattttatat aaatatttgc taaaagaggt agaaatgccg gcaaaaggaa aaaggtacac
     5881 aaaacaaggt catatattac cctcaaccca caaaagataa agaacaaaac gtattataac
     5941 ggtttaaaat ataacttcta ttcttataag atatggtgta aactgaactt ataaaagaaa
     6001 taattcattt gaattcctca agaaattatt atcattttac ataggctatg gtaaaaatac
     6061 gatagtcaat tagctagact tgctacctgg gatgcaaatc tagcaataat aatgaataat
     6121 ctaaggagga agagatgaaa aggatagtag tactcttact tgcttttgtt cttattttat
     6181 cactgggagc atgtgctggt aaagaggaga cccctgaggc tgtagcacca gaagcagaag
     6241 agcaaacaga aaagggcgac ctagcagatg gcatttattt tgccatggaa gatgaatttg
     6301 ctgatagtgg atggaaatct actgtaacac ttaaggtaga aggtggagaa attgttagtg
     6361 ccgattggaa tgccgttaat attttggggg gagtagacaa gaaaaccttc tctaaagacg
     6421 gcgattatgg tatgaaagaa tttggaaatg cccagtccca ctggcacgaa caagcacaag
     6481 aagtagaagc ttatttagtt caaacacagg atcctaccgc cattgaatac aaggatgatg
     6541 aaggacacac cgatgcaata gccggtgttt cagtccatgt caatgatttt tttgaactag
     6601 cgaaaaaagc actagataaa gagcctattg aactaggtca gtatgaagat ggagcttatc
     6661 atgctcaggc aaaagaattt gttaatggct ggaaggatac agtagatctt actgtattaa
     6721 acggacatat tctcagcgtt tcttgggatg gaataagtga agaagatcct gaaataacca
     6781 aaaaaacagc atctgctgat ggaagctatg gaatgaaaga agagggaaat gctcagtctc
     6841 attggcatga gcaagcccaa aaagcggaag cctatctact agaaactcaa gatcctagga
     6901 aaattgacta tagtgatgac cagggtcata cagatgccat ttctggagtt agtattggtg
     6961 ttaatggatt ttttgattta gccaaggaag cacttgaaaa ataatggata gaaaaaaggg
     7021 ttt
//
LOCUS       DASRNA010000205         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_70581,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000205 DASRNA010000000
VERSION     DASRNA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70581"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VFD08_05780"
     CDS             complement(<1..1066)
                     /locus_tag="VFD08_05780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZK10562.1"
                     /translation="MNTKYIFITGGVVSSLGKGITAASLGRLFKARGYKVAIQKFDPY
                     INIDPGRMSPYQHGEVFVTEDGAETDLDLGHYERFTDENLNRYSSVTSGKVYWRVLNR
                     ERQGEYDGATVQVIPHITDEIKRRVYILGEENDIVITEIGGTVGDIESLPFLEAIRQL
                     KYEMPGENILYIHMTLIPYLKKAEELKTKPTQHSVKELQSLGIQPHIIVCRSPMALSQ
                     EIKDKIGLFCNIPSNHVFHNLDAESIYEIPLYLERDGLADKACAILDLPNCQPELGDW
                     EEIVKKDLGRQKKVKIALVGKYVELKDAYISTCEALEHAGIELGLRPELVWINAEELS
                     ASNSRELLSSVHGLILPSGFG"
     gene            complement(1213..1683)
                     /locus_tag="VFD08_05785"
     CDS             complement(1213..1683)
                     /locus_tag="VFD08_05785"
                     /inference="COORDINATES: protein motif:HMM:NF018410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HZK10563.1"
                     /translation="MKSYLIAILLSMMPISEVRGGIPYAMSQGIPFPLSFLLPMLANM
                     LIVFPLLFMVKPLFAFLRRYHIFERLIDAYEGRAERKMNKHMAFKKWGLFIFVAIPFP
                     TTGVYTGVVASILAGMRARESFFPILLGVLVASILTYMAASGLLGAFNLIFTKM"
     gene            complement(1680..>2488)
                     /locus_tag="VFD08_05790"
     CDS             complement(1680..>2488)
                     /locus_tag="VFD08_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZK10564.1"
                     /translation="GADYVELVDQALKGRWIDVDERPGKRSGAYSSGCYDTAPYMLLN
                     YQDNLSNFFTLAHEMGHSLHSYFSSREQDYTYASYGIFLAEIASTFNEALLNHYLLEK
                     EEDPNIRLYLINHYLETFKGTLFRQTMFAEFERDIYARVEAGQGLSSEDFSRSYGDLN
                     DKYFCSKVEVDEDISHEWSRIPHFYYNFYVYQYATGLSAATSFAKRVLDQQEGALAGY
                     RGFLAAGSSKYPVDVLLEAGLDMRSPQPVKEALEIFSSLVDEFETLLEKK"
BASE COUNT          686 a          579 c          538 g          685 t
ORIGIN      
        1 ttccaaaacc actgggtaaa ataagaccat ggaccgagga gagtagctcc ctactattag
       61 aagcacttag ctcctctgca ttaatccaga caagttctgg ccttaggccc agctcaattc
      121 ctgcatgttc tagggcctcg caggtagata tataggcgtc ttttaattct acgtattttc
      181 ccaccagggc aatcttaacc tttttttgcc gccccaggtc tttttttaca atttcttccc
      241 agtcgcctag ttcaggctgg cagttgggaa gatctaaaat ggcgcaggcc ttatcagcta
      301 gcccatcgcg ctctaggtaa agtgggatct catagatgct ttctgcatct agattatgaa
      361 aaacatgatt ggaagggata ttgcaaaata ggccaatctt atcctttatt tcctgggata
      421 gggccatggg gctgcggcaa acaataatat ggggctggat acccaggctt tggagttcct
      481 tgaccgagtg ctgggtgggt tttgtcttta attcctctgc cttttttaga tagggaatca
      541 gtgtcatatg tatatagaga atattttctc ctggcatttc gtatttaagt tgtcgtattg
      601 cttctaaaaa aggcagagac tcaatatctc ctacggttcc accaatttct gtgataacaa
      661 tatcgttttc ttctcccaga atatagactc ttcttttgat ttcatcggta atatgaggaa
      721 taacctggac ggtggctcca tcatattctc cttgccgctc cctattaagg acccgccagt
      781 agaccttgcc tgaggtcact gaggagtagc ggttgaggtt ttcatcggta aatctttcat
      841 agtggcctag atctaggtct gtctctgcgc catcttctgt cacaaagacc tccccatgct
      901 ggtagggact catccgaccc ggatcaatat tgatataggg atcaaacttt tgtatggcca
      961 ccttatagcc cctggcctta aaaaggcggc ccagggaggc ggcggtaatg cccttgccca
     1021 gggaggaaac aacaccccct gtaataaaaa tatattttgt attcataatt tctccaaaaa
     1081 aaaggtgata agtatcctag gaaggatgac ctaccacaag ggtgaaggca cccctgccag
     1141 ggtgcgcaaa taactcctac caagtataac aaagaaaaag aaggctggtc aagctaaagc
     1201 ccctctcttt ttttacatct ttgtaaagat tagattaaag gcccctagaa gtcccgatgc
     1261 tgccatatag gttaggatgc tggctaccaa aacccccagc aggatgggaa aaaaggattc
     1321 tcttgccctc atgcccgcca aaatactggc aactactcct gtatagactc ctgttgtggg
     1381 aaagggaatg gcaacaaaga taaaaaggcc ccatttttta aaggccatat gcttgttcat
     1441 ttttcgctct gcccggccct cataggcgtc aatcagccgc tcaaaaatat gatatcttct
     1501 taaaaaggca aaaagaggct ttaccataaa aagaagggga aaaacaatca gcatatttgc
     1561 cagcatggga agaagaaagg aaaggggaaa gggaattccc tggctcatag cgtagggaat
     1621 tcctcctcta acttcactaa tgggcatcat agatagaagt atagcaatta aataactttt
     1681 cattttttct ccaaaagggt ctcaaattca tctactaggc tgctaaagat ttccagggct
     1741 tcctttactg gctggggcga gcgcatatca agtcccgctt ccagcagaac atctacgggg
     1801 tatttgctgc ttcctgctgc cagaaaacct ctataacctg ctagggcccc ctcctgctga
     1861 tccagaactc ttttagcaaa gctggtcgca gcgcttaggc ctgtggcata ctggtagaca
     1921 taaaaattat agtaaaaatg cgggatccga ctccactcat gactgatatc ttcatctact
     1981 tctaccttgg agcagaagta cttatcattt aagtccccat agcttctgct aaagtcttca
     2041 ctactaagcc cctggcccgc ttctaccctg gcataaatat ctctttcaaa ttcagcaaac
     2101 atggtttgcc taaaaagagt gcccttaaag gtctctagat agtgattaat caaataaagg
     2161 cggatatttg gatcttcttc tttttctaac aggtagtggt tgagcagggc ttcattaaag
     2221 gtagaagcaa tctctgctaa aaagatccca tagctagcat aggtatagtc ctgctcccta
     2281 gaagaaaagt agctatggag ggaatgaccc atttcatggg ctagggtaaa gaagttggat
     2341 aggttgtcct gataatttag cagcatatag ggcgctgtgt catagcagcc actggaatag
     2401 gcgccactgc gcttgccagg acgctcatct acatcaatcc agcggccttt tagggcctgg
     2461 tctacaagct ccacatagtc ggctccca
//
LOCUS       DASRNA010000206        12812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_584641,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000206 DASRNA010000000
VERSION     DASRNA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584641"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFD08_05795"
     CDS             complement(<1..983)
                     /locus_tag="VFD08_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK10565.1"
                     /translation="MERENNKVSGSKSSLATIAPYARYIGRRLLSLIPVLIVISIVVY
                     ILINLMPGDPVTAMLDPQMTKTMTPDERAKYVETMRELLGYDKGLVARYFIWMRDTFI
                     RGDFGYSVRYNKPVNEIIWTYIARSFKINIWGFLLAFLISIPVGIQAAVKKNKFFDKS
                     VTVLTIVGISLPSFFLALLLIMLFTIALGWLPFSGMSDPRGVVPAWKYLVLPITVVVL
                     TSLASLIRYIRSAMVDVLDADYVRTARAKGLKEKVVIYRHAFQNALIPVVTLIGMYIP
                     SLFGGSIVVERIFAYPGMGFLLGNAYSYKDRAVLQTALLFFGLLTLLGNIF"
     gene            complement(1063..2916)
                     /locus_tag="VFD08_05800"
     CDS             complement(1063..2916)
                     /locus_tag="VFD08_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK10566.1"
                     /translation="MKRKITVFLLLALLLTSVLAGCGGTEEAAEEVEDAVEEVVETDE
                     PAEEEEEEEVAVDGDRPFVVGTAEFSGDFHNGWSNSSYDNNIRKLVWGYGLMMSTPEG
                     DLVDSHLVEEKTVSDDLYEWTFKLKEGLEFHNGEELSASDVKFTYDFYMDTEPLGATG
                     ATSNLGDYVADIEVVDEQTVKFTLEKPIYTVDLNVFMTYILAEDTITAGAEAEGFNVQ
                     EYVKAHISEPIGYGPYKLVEYADSEYVILEANQDHVGVVPAIDELIVKYTPSETELDQ
                     LLQGEVDLLHEQVEAEKIDAAKADANFQYNNYFRHGGGTIVLHTDFDATQLTEVRQAF
                     AYLLNRPKIIELFLGEYGIASNGPYSKNDWMMYDDDEMDIFATEAVGKFEATLNNYDI
                     VNEDGSFDEEANIAKAHELLDAAVAKTDGAYAMLTGNAADGYMWDGEPMVLKITYTDF
                     WADTYNIVWNDEYVSKLGFDVQLTGLDWPVMYGHWIGDTAEEREYHAYVGGISYALKS
                     NPRLDYSTKEILPWGQPSNNGPRFSGGSSYTPEEWDQLLADIENADPLTGRDEYRENW
                     RKYITVMNEEVPIIPVYSNNYHDLYTIDLENFETNALWDWPMAIVNANWAN"
     gene            3448..3831
                     /locus_tag="VFD08_05805"
     CDS             3448..3831
                     /locus_tag="VFD08_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10567.1"
                     /translation="MKEYYCRNCFRTYTEEVCPSCGAKGSLAGSEDPIFFMEAGYFLS
                     PRVEDFLTEKGIPFLKKGELGAGLTTRIGLSSEHNHYFIPLKAYKDFEKELILFTALF
                     SLIFSYYPSDKLLFIFSKVQQLHCK"
     gene            complement(3803..5548)
                     /gene="pyk"
                     /locus_tag="VFD08_05810"
     CDS             complement(3803..5548)
                     /gene="pyk"
                     /locus_tag="VFD08_05810"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZK10568.1"
                     /translation="MERRTKIVCTLGPATDDPEILEAVIRAGMNVARFNFSHGNHQDH
                     KKRMDMVKEIREKLDLPVAILVDTKGPEIRLGKIKEGIFLKEGAPFILTGESIVGDEN
                     RAAISYKKFYKDLEVGNNILIDDGLISLRVEKIDEKRNIHTLVITGGELMSNKGINAP
                     SSRLNLPALTTQDKADIRFGLSQGVDFIAPSFVCTKKNVLDIRKILEEEDASHVQIIS
                     KIENRQGFVNIEEILQVSDGIMVARGDLGVEMPIEEVPMAQKVMLKLCNAMGKPVITA
                     TQMLDSMIRNPSPTRAEATDVANAIFDGTDAIMLSGETAAGKYPVESVRVMDAIARKT
                     EEALPMYSMERDYEQYHDSVTFAVSKATVEAAQSLDVKAILTVTMSGFTARKLAMYRP
                     DCPIIALTPKKEIRRRLSLSWGINSLDLEELFSTDQMFREMAEVSKEAGLVEEGDMVI
                     ITSGLPLGIAGATNLMKIHVVGEIMQKGIGIGHSSIKGRIRFAQNLHIEPLQKGDILY
                     LEEVVPNDLPLVKQAAGLITAEGGYTSLAAEWARELGIPAVLGVRLYRSRIDDGHEIS
                     LDAKLGIIYNAVAVL"
     gene            complement(5550..6512)
                     /gene="pfkA"
                     /locus_tag="VFD08_05815"
     CDS             complement(5550..6512)
                     /gene="pfkA"
                     /locus_tag="VFD08_05815"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZK10569.1"
                     /translation="MKKTIGVLTSGGDAPGMNAAVRAVVRTAKYHKLDVYGIRRGFRG
                     LLEQDYVVLNSRSVSDILQKGGTFIKSARSPEFNSEEGVKKAAGIINDLALDALIVIG
                     GDGTIRGAAELAKQGVKVIVLPGSIDNDLGYTDYTIGFDTAITTVMNAMSNLRDTSSS
                     HGRVFVVQVMGRECGDIALHAGIVSGAEEVIVPEMPFDMEEVTANIRKAMARGKSHYI
                     ILLAEGAADPFTFTEEIQKLSGINTRLNIIGYLQRGGSPSSFDRMLATRLGVHAVELF
                     LEGKHGIAVGIRGNELVHENLTKAIEARDSLDKDLYEMAKILAV"
     gene            complement(6526..9894)
                     /locus_tag="VFD08_05820"
     CDS             complement(6526..9894)
                     /locus_tag="VFD08_05820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZK10570.1"
                     /translation="MKPFVHLHLHTEYSLLDGFCPLEELVLSAKKKGFSHLAITDHGV
                     MYGVVDFYKMCKKHGIIPIIGCELYVSTDNPKVNHHLVLLVKDEQGYKNLIKMVSRSF
                     TEHFYYRPRVTKELLKEHSQGLIALSSCLQGEISQMLLQGRDKDALKVAYDYKQIYGD
                     DFYIELQDHGLAEQKRILPRQIELHKQTGIPLVATNDVHYVEKEDYLAQDVLLCIQTG
                     SEFKDKDRLRFPAKEFYLKDSEQMYQLFSYVPQALENTKKIADKCNFDFDFSTMHLAP
                     FSEETDFDAKKTLHDLCYQGLDRLYGHDKHKERLEEELGIIDSMGYNDFFLIVADFVS
                     FARNSGIFVGPGRGSGGGSLAAYCLGIHRVDPMKYGLLFERFLNKDRVTMPDFDIDFE
                     DERRQEVIDYVAHKYGSARVAQIITFGTFGARQAIRDVGRVFGMDYAEVDRVAKAIPP
                     SPGMTLERGIKESTRLRELMKKEEVSLLIDMARRIEGRPRHCSTHAAGVVMAGKALEE
                     IIPLAKHEGALVTQYNMNLLEELGLLKMDFLGLRNLTIIKQALRLIEENKGVQIDIDQ
                     IPTDDKETFALLGRADTLGIFQLESTGMRSFFREIKPHSIEDIIAGISLYRPGPMDQI
                     PVYIRNKHNPELISYPHESLKPILEVTYGVMVYQEQVMSMVRTLAGYNYNQADIIRRA
                     MSKKKMDVMAREKQRFLYGDGKEIHGAIKKGLSEKEAQNLYQTMTEFANYAFNKSHAA
                     GYALIAYQTAYLKTHYPVEFMAALMSSVMGNSVKLASYMETCRSMGIEILPPHVNHST
                     QGFSTEEGAIRFGLKAVKNVGGLLIESLLEARKSGPFKNFEELLQRLSSTSLNKRAFE
                     SLIKAGALDGLGESRRSLLQRYEVQIDGLQRSERFNALGQISLFDDLIPTLTTALNVK
                     ELDEDQLLLYEKEVLGMYLSGHPLSKYAPLRPSNFDLSTLAELSEEEGEGKRVELLLM
                     KKSIRRKMTRTSKTMAFVEMEDEMDSIEVIFFPRTFKDYEAVLEKEQYFLIRGKLQMK
                     EDEKPNLICEGLKALTKAPSKKILYIRVEQFEDKKKADIIGLFKANPGSCRVRIYEME
                     TGELREMKGTFVGTSQAFIDELKRLLGADNVVIK"
     gene            complement(9891..10772)
                     /gene="whiA"
                     /locus_tag="VFD08_05825"
     CDS             complement(9891..10772)
                     /gene="whiA"
                     /locus_tag="VFD08_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HZK10571.1"
                     /translation="MSYSAKLKEDLILQAEEDGLEALLSGFLRCSYQEDRFSSDLATL
                     IRYVYLKLQERGIESNIGIKKSSYSKLNRYYLDFLEKEDLKKLHVIDDQGLVEHIPGI
                     YYSQMKLQRSYLKGAFLACGSISDPKRSYHLEMTFRDQPMAIAIKNLCEHFNLAAKII
                     YRNQWVVYIKSSQSIVDFLLLMGASSQAFALEDIKILRSMKNDVNRLVNCETANINKT
                     VEASLRHLRAIELIQNTIGLAALSKPLQEIALCRWEEPELSLKELGERLDPPLSKSGV
                     NNRLSRLLKIAQKLEES"
     gene            complement(10781..11167)
                     /locus_tag="VFD08_05830"
     CDS             complement(10781..11167)
                     /locus_tag="VFD08_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZK10572.1"
                     /translation="MIISAGGVVISESKVLLLQNTKGKWVLPKGHVEENESLRRAAVR
                     EVKEESGVEAKIQKKLGWVEYSFYHQGRSQNKKVLWFKMQKVGGTPEPLHEEGFIDLV
                     FFSQEDLPKSHMHENELLMVEKAFGC"
     gene            11371..12579
                     /locus_tag="VFD08_05835"
     CDS             11371..12579
                     /locus_tag="VFD08_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZK10573.1"
                     /translation="MERLQGTVVRGLRTPIFKEGDNLVEEISHLLMRFYLVEQVKPKN
                     KDIISITESVVARAQGNYAHIDDIARDFHSKFPENEAVGIIFPILSRNRFATSLKGFA
                     RGAAMGNREVILMLSYPSDEVGNSLAFTEVLEKLGVNPWTDVLSEREYRAIFGEFHHL
                     FTGVDYVEFYRQILESEGVNCQVVFANIATSILPYTKNVLNCDIHSRDKTKDLLCKAG
                     AKKVLTLAEILNKANTSHGYNEEYGLLGANAATENTIKLFPRDCQRLVMDLQARMKEL
                     TGKLIEVMVYGDGAFKDPVGQIWELADPVVSPGFTPGLSGTPSEVKIKYLADNTFSHL
                     KGDDLKGAIKNHLAKILEEDISQAESLGTTPRRLTDLIGSLSDLTSGSGDKGTPLVYI
                     QGYFDKFSID"
BASE COUNT         3656 a         2891 c         2332 g         3933 t
ORIGIN      
        1 ataaatatat ttcctaataa ggtcagaagt ccaaaaaaca gtagtgcggt ttgtaatacc
       61 gctctatctt tatatgagta agcattacct aataaaaacc ccatgcctgg ataggcaaag
      121 attctttcaa caacaatcga gcccccgaat aaactaggga tatacatacc aataagtgta
      181 acaacaggta taagagcatt ttggaaagcg tgcctatata taacgacctt ttcttttaag
      241 cccttggctc tagccgttct gacataatca gcatctaata catcaaccat ggcagatcga
      301 atatatctaa ttaagctagc aagtgatgtt aaaacaacaa ctgttatcgg taaaactaag
      361 tatttccaag caggaacaac acctcgagga tctgacattc ctgagaaggg aagccagcct
      421 aatgctatgg taaaaagcat aattaataat aaggctaaaa aaaacgaggg tagtgatata
      481 ccaactattg tcagaacagt taccgattta tcaaaaaact tgtttttctt tacagccgct
      541 tgaattccaa cgggtataga aataagaaag gctaatagaa atccccaaat attaatttta
      601 aaggatctag ctatataggt ccaaattatc tcatttactg gcttgttata tcttactgaa
      661 taaccaaaat caccccttat aaaggtgtcc ctcatccaga taaaataacg cgccacaagg
      721 cctttatcat aacctaacaa ctctctcatc gtctctacat actttgctct ttcatcagga
      781 gtcatggtct tggtcatttg aggatccagc atagcagtta ccgggtctcc tggcattaga
      841 ttaattaaaa tatatacaac aatggatatt acaattaata ccggtataag agatagtaat
      901 cttcttccga tatagcgagc atatggagcg attgttgcta gagatgattt agagccacta
      961 actttattgt tctccctctc catagataac ctcttttctt aaaagataag gccacgttag
     1021 ttaaaccgtg gccttattag aataattaaa cttagttggc ttttagtttg cccaatttgc
     1081 atttacgatt gccattggcc aatcccataa tgcatttgtt tcaaagtttt ctaagtctat
     1141 cgtatagagg tcatggtagt tatttgaata tacaggaatg attggaactt cttcgttcat
     1201 tactgtgata tactttctcc aattttctct atattcatct ctacctgtta atgggtctgc
     1261 attttcaata tctgctaata attgatccca ctcttcagga gtataggatg atccaccaga
     1321 aaatcttggc ccgttattag atggttgacc ccaaggtaaa atttcttttg ttgaatagtc
     1381 aagtcttgga ttagatttaa gagcatagct tataccacca acataggcat ggtattctct
     1441 ttcttctgcc gtatcgccta tccagtgacc gtacattact ggccagtcaa gtccagttag
     1501 ttgcacatca aatccaagtt tagaaacata ttcgtcattc caaactatat tgtaggtgtc
     1561 tgcccagaag tctgtatagg taatcttaag aaccattggc tcgccatccc acatgtaacc
     1621 atctgcagcg ttacctgtaa gcatagcata agcaccgtct gtcttagcta cagcagcatc
     1681 aagaagttca tgtgcttttg caatgttggc ttcttcatca aagcttccat cttcattaac
     1741 aatatcatag ttatttagtg tagcttcaaa tttaccaact gcttctgttg caaatatatc
     1801 catctcatca tcatcataca tcatccagtc gtttttagag taaggcccat tagaggcgat
     1861 accgtactca cctaggaata gttcgatgat ttttggtctg ttaagaaggt aagcaaaggc
     1921 ttgacgcact tctgtaagtt gtgttgcatc aaagtcagtg tgcagaacga ttgttccacc
     1981 accatgtcta aagtagttgt tgtattggaa attggcatca gctttagctg catcaatttt
     2041 ttccgcttca acttgttcat gtagtaggtc aacttcacct tgtagtaatt ggtcaagctc
     2101 tgtttcactt ggagtatact taacaattaa ttcgtcaatt gctggtacaa caccaacgtg
     2161 atcttggttg gcttctaaaa taacatattc agaatctgca tattcgacca gtttatatgg
     2221 tccatatcca ataggttctg agatatgagc tttaacatat tcttgtacat taaacccttc
     2281 agcttcagct ccagcagtaa ttgtgtcttc tgctaaaata tatgtcatga agacgtttag
     2341 gtcaactgtg taaatgggct tttcaagtgt gaatttaact gtctgctcat ctacaacttc
     2401 aatatcagca acatagtcac ctaggttaga tgttgcacca gtcgctccta gaggctctgt
     2461 atccatatag aagtcatatg tgaacttaac atcactcgca gataactctt caccattgtg
     2521 gaactcaagt ccttctttaa gcttaaaggt ccactcatat aggtcgtcac ttactgtttt
     2581 ttcttcaacc aggtgtgagt caacaagatc gccttcgggt gtactcatca taagaccgta
     2641 tccccaaact agcttacgaa tgttgttatc atagcttgaa tttgaccaac cattatggaa
     2701 gtcaccgcta aattctgcag ttcctacaac aaatggtctg tctccatcta cagctacttc
     2761 ttcctcttct tcttcttctg ccggctcgtc ggtttcaact acttcttcaa ccgcatcttc
     2821 cacctcttcg gcggcctctt ctgtaccacc acagcccgca aggactgaag ttagaagtaa
     2881 agccagcaat aagaaaactg taatcttcct cttcatacat ttcctcccat tcttagatta
     2941 ttggaacacc tactcatcgc ttttttaagc acaaaatcca cctgcaactt ataagtatgc
     3001 caattttggt atagcgtaat cgcttactac acagattata tgattaatcc taagataagt
     3061 caagaatatt ataccttgcc ctataggcaa aatataaaaa gtttatcttt cgccgttttt
     3121 acaaagtaat tgcaatatat aggcgaaatt tctaccataa tatctcaatc tccagaaaaa
     3181 ttcagtaaat ataaaggaaa aaagcctata gcagctttct aactttttag gggtcactta
     3241 aaggctaatc tctaggccct aaaaaccaaa gaaagtagga gtttcactct taatatattc
     3301 cttctagcaa gaattgtcct taggctggga agaaaaaata ttttcttcct attagagctt
     3361 ctccttttac aaccctgcaa gaagaacttt tctgatacaa tagatacaaa agggaaggga
     3421 ggatgggtaa tatactcaaa taattatatg aaagaatatt attgtcgcaa ctgttttagg
     3481 acttatacag aggaggtctg cccttcctgc ggcgccaagg gaagcctagc aggctcagaa
     3541 gatcctatct tttttatgga agcaggttac ttcctctccc cccgtgttga agacttccta
     3601 actgaaaaag ggattccctt tctaaaaaaa ggagagcttg gagcaggcct aaccacaaga
     3661 atcggtctta gttctgaaca caaccattac tttattcctc taaaagccta taaggacttt
     3721 gaaaaggaac ttatactttt tactgcttta ttctctctta tcttttcata ttatcctagt
     3781 gacaagcttc ttttcatctt ttctaaagta cagcaactgc attgtaaata attcccagct
     3841 ttgcatctag gcttatttca tggccatcat caatccggct tctgtaaaga cgcacgccta
     3901 gcacggcagg aatgcctagc tctcttgccc attctgcagc taaagaggta tagcctccct
     3961 cggcggtaat aaggccagct gcttgtttaa ccaggggcaa atcattgggt accacttcct
     4021 ccaaataaag aatatcgccc ttttgaagtg gctcaatatg caaattttgt gcaaatctta
     4081 ttcttccttt aatagagctg tggccaatgc ctataccctt ttgcattatc tctcccacca
     4141 catgaatctt cataagattt gtggctccag caattcctaa aggaaggcca gaagtaataa
     4201 taaccatatc tccttcttca accaaacctg cttctttgga aacttctgcc atttctctaa
     4261 acatttggtc cgtactaaaa agttcttcta gatccaggga gttaataccc cagcttaaag
     4321 aaagtctgcg acggatttct tttttgggtg taagggcaat aataggacag tcaggacgat
     4381 acatagccaa cttcctagcg gtaaaaccac tcatggttac tgtaagaatt gccttaacat
     4441 ctaggctttg tgcagcctct actgtagcct tacttacagc aaaggtaacc gagtcatggt
     4501 actgctcata gtcgcgctcc atagaataca taggaagggc ttcttcggtc ttgcgtgcga
     4561 tggcgtccat cactcgcaca gattcaaccg gatacttccc agcagccgtc tctccactta
     4621 gcataattgc atcggtacca tcaaagattg cattggccac atcagtggcc tctgcgcgag
     4681 taggacttgg atttcgtatc atactatcta acatctgggt tgcggtaata acaggtttac
     4741 ccatagcatt gcaaagctta agcataacct tctgggccat ggggacttct tcaatgggca
     4801 tttctactcc aagatctccc cgggctacca taatcccatc gctaacctgc aatatttcct
     4861 caatatttac aaaaccctga cgattttcta tttttgagat aatctgtaca tgggaagcat
     4921 cttcttcttc cagtattttt ctaatatcca agacgttttt ctttgtacat acaaaggagg
     4981 gagcaataaa atctacccct tggcttaggc caaaacgaat atctgcctta tcttgggtgg
     5041 taagggccgg aaggttaagt cgactagagg gggcattgat tcctttattg ctcataagct
     5101 ctccgccagt aatgaccagg gtatgaatat ttcttttttc atcaattttt tccaccctaa
     5161 gagaaatcaa accatcatca ataaggatgt tgtttcccac ttcaaggtct ttataaaact
     5221 tcttataact aatggctgct ctgttttcat ccccaactat gctttcccca gtaaggataa
     5281 agggggctcc ctctttaaga aaaattcctt cttttatttt ccctaaacgg atttcaggac
     5341 ccttggtgtc aacaagtatg gctactggta aatcaagttt ttctcttatt tcttttacca
     5401 tatccattct ttttttatgg tcttgatggt ttccatggga aaaattaaaa cgagctacat
     5461 tcatccctgc ccttataaca gcttctaata tttcagggtc atcggttgcc ggacccagag
     5521 tgcatacaat ttttgttctt ctttccattt tacaccgcca aaatctttgc catttcgtag
     5581 agatccttat ctaaggaatc tctcgcttcg atggcctttg ttaaattctc atgaacaagt
     5641 tcatttcctc ggattcccac ggcaatgcca tgctttcctt ctaaaaatag ctccactgca
     5701 tgtacgccta ggcgagtagc cagcattcta tcaaaactag aagggctccc tcccctttga
     5761 aggtaaccta taatgtttaa gcgagtattt attccactga gtttttgaat ttcttcggta
     5821 aaggtaaatg gatccgctgc tccctcagct agcaaaataa tataatgact ctttccccga
     5881 gccatagcct ttctaatatt ggctgttact tcttccatat caaagggcat ctcaggcaca
     5941 attacttcct ctgcaccaga tacaatccct gcatgaagag caatatcacc gcattctctt
     6001 cccataacct gaaccacaaa gaccctgcca tgagaactgg aagtatccct taagttactc
     6061 attgcattca tcactgtagt aatggccgta tcaaagccga tagtataatc tgtataaccc
     6121 agatcattat caatacttcc aggaagaaca atcaccttaa ctccttgctt ggccagttcg
     6181 gctgctcccc taatggttcc atctcctcca attactatca gggcatcaag ggctaagtca
     6241 ttgattatgc ctgctgcttt ttttacgccc tcttctgaat tgaattccgg acttctggct
     6301 gattttataa aggttcctcc tttttggagg atatcgctaa cactgcgaga gtttagtacc
     6361 acgtagtcct gctcaagaag tccacgaaaa ccccttctaa tcccatagac atcgagtttg
     6421 tggtacttgg ctgtacgcac aacagctcta acagcagcat tcattcccgg ggcgtcgcca
     6481 ccgcttgtca acactccaat tgtttttttc atatcttttc cctctttatt ttattaccac
     6541 attatctgca ccaagaagtc ttttgagttc atcaataaag gcttgcgaag tgccaacaaa
     6601 agtgcctttc atttctcgta gttctcctgt ttccatctca tatatgcgta cccggcaaga
     6661 acccggattt gctttaaata gtcctataat atctgctttc tttttatcct caaattgctc
     6721 cacccttata tagaggattt tctttgaggg ggcctttgtt agagctttta gcccttcaca
     6781 aattaaattg ggtttttcat cctctttcat ttgaagctta ccacgaatca aaaagtattg
     6841 ttctttttcc aaaacagcct catagtcttt aaaggttcgc gggaagaaga taacctctat
     6901 actatccatc tcatcttcca tctcaacaaa ggccatggtc ttagatgtcc tggtcatttt
     6961 tcgtctaatg ctttttttca tcaaaagaag ttccactctt ttgccctcgc cctcttcttc
     7021 actaagttct gccagggttg aaaggtcaaa gttacttggc ctcaagggag catatttgga
     7081 cagtggatga cctgacaagt acatgcctag cacttccttt tcataaagaa gaagctggtc
     7141 ctcatctaat tcctttacat ttagggcagt agtaagggta ggaattagat catcaaaaag
     7201 gctaatctgc cctagggcgt taaagcgctc tgatctttga agcccatcta tttggacctc
     7261 atagcgttgg agaagacttc ttctgctttc accaagacca tctagggctc ctgccttaat
     7321 caggctctca aaggcccttt tatttaagct agtagaactt agtctttgaa gaagctcttc
     7381 aaaattctta aagggtcccg actttcgcgc ttcaagcagg ctttcaataa gaagtccgcc
     7441 cacatttttt actgccttaa gaccaaagcg gatggctcct tcttctgtag aaaagccctg
     7501 cgtcgaatgg tttacatggg gaggtagaat ctctatcccc atagacctgc aagtttccat
     7561 ataagaggct agctttacgc tattgcccat aaccgaactc ataagagctg ccataaactc
     7621 cactggatag tgggtcttaa gataggccgt ttgataggct ataagggcat aacccgctgc
     7681 atgactctta ttaaatgcat agttggcaaa ttctgtcatt gtctgataaa gattttgtgc
     7741 ttctttttcg ctaagacctt tttttatggc tccgtgaatt tcttttccat cgccatataa
     7801 aaacctttgt ttttccctag ccataacgtc catttttttc ttactcatgg ctcgtctaat
     7861 aatatctgct tgattataat tataacctgc cagggtccgg accatactca taacctgttc
     7921 ttgatagacc atgaccccat aggttacttc taaaattggt tttagcgact catggggata
     7981 ggaaataagc tccggattgt gcttatttcg aatgtatact ggaatttgat ccataggtcc
     8041 tggtctataa agacttatac ccgcaattat gtcttcaata gagtggggct ttatttctct
     8101 gaaaaaacta cgcatacctg tgctctcaag ttgaaaaata cccagggtat ctgcccttcc
     8161 aaggagggca aatgtttctt tatcgtccgt agggatctgg tcaatgtcga tctggacgcc
     8221 cttattctcc tcaataagcc taagggcctg ctttataatg gttaaatttc taagccctaa
     8281 aaaatccatc tttaaaagcc ctagttcttc tagaagattc atattatact gggtcactag
     8341 agcaccctca tgcttggcta gaggaataat ttcttctagc gcttttcctg ccataacaac
     8401 gccagcagca tgggtagaac agtggcgcgg ccgtccctct atgcgccggg ccatatcaat
     8461 aagaagagat acctcttctt ttttcataag ctctcttagt ctagtgcttt cctttatccc
     8521 tcgctctagg gtcatacctg gactgggggg aatcgccttg gccaccctat ccacctctgc
     8581 atagtccatt ccaaagacgc gacctacatc gcgaatagcc tgacgggctc caaaggtacc
     8641 aaaggtaata atttgagcca cccttgcgct gccatattta tgggcaacat aatctatgac
     8701 ctcttggcgg cgctcatctt caaaatctat atcaaagtct ggcatggtaa ctcggtcttt
     8761 atttaagaaa cgttcaaaga gaaggccgta tttcatagga tctactcggt ggatgcccag
     8821 gcaataggcc gctagacttc ctcccccgct gcctcttcct gggcctacaa agatgccact
     8881 atttctagca aaggaaacaa aatccgctac aataagaaag aaatcattgt agcccatgga
     8941 atctataata cctagttctt cctccagtcg ctctttatgc ttatcatgcc catagagcct
     9001 atcaaggccc tggtagcaaa gatcatggag ggtctttttc gcatcaaagt cggtttcttc
     9061 tgaaaaaggg gccagatgca tggtggaaaa atcaaagtca aaattgcatt tgtccgcaat
     9121 tttttttgtg ttttcaaggg cttgcggcac ataagaaaaa agttgataca tctgctcaga
     9181 gtcctttaag taaaactctt ttgcaggaaa acgcagccgg tctttatcct taaactcaga
     9241 gcccgtctgt atacaaagta agacatcttg ggccaggtaa tcctcttttt ccacatagtg
     9301 gacatcattt gtggccacta ggggaatgcc tgtttgttta tggagctcga tctgcctggg
     9361 aagaattctt ttttgttctg caagaccatg gtcttgcagc tcaatataaa aatcatcccc
     9421 ataaatctgc ttatagtcat aggccacctt tagagcgtcc ttgtcccttc cctggagcaa
     9481 catttgggaa atttccccct gtaaacagga agaaagggca atcagtccct ggctgtgctc
     9541 tttaagaagt tcctttgtca ccctgggcct atagtagaaa tgttcagtaa aacttcggct
     9601 aaccatcttg attaagtttt tatagccctg ctcatcttta acaagaagga ctaaatggtg
     9661 atttaccttg ggattgtctg tacttacata aagttcgcaa ccaataatag gtataatacc
     9721 atgtttttta cacattttat aaaagtcaac caccccatac attactccat gatcggtaat
     9781 ggcaagatgg ctaaatcctt ttttctttgc cgacaaaacc aattcctcta ggggacaaaa
     9841 gccatcaaga aggctgtatt ccgtatgcaa atgaaggtga acaaagggct tcatgactcc
     9901 tccagttttt gcgcaatctt aagcagtcga ctaaggcggt tgtttacacc agacttactc
     9961 aggggtgggt ctagacgctc tcctagttcc ttaaggctta gttctggctc ctcccagcga
    10021 catagggcaa tctcttgcag gggcttagaa agggctgcca ggccaatggt gttttggatc
    10081 aactcaatgg ctcttaggtg tctaagagag gcctctaccg ttttatttat attggccgtt
    10141 tcacaattaa caaggcggtt tacatcattt ttcatagacc gcaaaatttt tatatcctct
    10201 agggcaaagg cttgactaga ggcccccatt agtagtaaaa agtctacaat gctctgggag
    10261 ctcttaatgt agacaaccca ctgatttcta tatatgattt tagcagctag attaaagtgt
    10321 tcacaaaggt tttttatggc gattgccatt ggctggtctc taaaagtcat ttctagatga
    10381 tagcttcttt tagggtcact tatagagccg caggccaaaa aagccccctt tagataggac
    10441 ctttgcaatt tcatctggga ataataaatt cctggaatat gctcaacaag gccctggtca
    10501 tctataacat ggagcttttt aagatcttcc ttttccagaa aatctaaata atatctatta
    10561 agcttagaat agctgctttt tttaatcccg atatttgact caataccccg ctcttgtaac
    10621 ttcaaataaa catagcgaat gagtgtagca agatcagaac taaacctatc ctcctgatag
    10681 gagcaacgaa gaaagcccga aagtagggcc tctagcccat cttcttctgc ttgaagaata
    10741 agatcttctt taagttttgc ggaataagac atacttcctc ctaacagcca aaggcttttt
    10801 cgaccataag cagttcattt tcatgcatat ggcttttagg aagatcttcc tggctaaaaa
    10861 acaccagatc aataaaacct tcttcatgta atggctcagg tgtcccacca actttttgca
    10921 tcttgaacca aagtactttt ttattctgac ttcgtccttg atgataaaaa ctatattcaa
    10981 cccatccaag ctttttttga atcttagctt ccacacccga ttcttcttta acctctcgca
    11041 ctgctgcgcg tctaagggat tcgttttctt ctacatgacc cttgggaaga acccatttac
    11101 ccttggtatt ctgaaggaga agaaccttgg actcactaat aacaacaccg cctgcactta
    11161 taatcatgct gcctcctgga atctattata ccctaagaaa aggggctagc acaaacttat
    11221 tgccttgacc caagtaaatc aaccctcttc tagggcaagg gcctccaaag aagctggcaa
    11281 caaagcttgt agaagcttgg ttcatcttta cctccttctt tgtatctgat acaatgtaag
    11341 cgaatataag gctaagaaat gaggtgcaaa atggaaagac ttcaaggcac agtagtacgt
    11401 ggtctacgga ctccaatttt taaagaaggt gacaatctag tagaagaaat cagtcatctt
    11461 ttaatgaggt tttatcttgt agaacaagta aaacctaaga acaaagatat tatttccatt
    11521 acagaatccg ttgttgcaag agcccaaggc aactacgctc atatagatga tattgctagg
    11581 gactttcata gtaaattccc cgaaaacgaa gccgtaggca tcatttttcc tatactcagc
    11641 cgcaatcgtt ttgccaccag cctaaagggc tttgcccggg gtgcagcgat gggaaataga
    11701 gaagttatcc taatgctaag ttatccctcg gatgaagtgg gcaactccct ggcctttact
    11761 gaggtcttag aaaagctggg agtcaacccc tggacggatg tcctaagcga aagggaatat
    11821 cgagcaattt tcggtgagtt ccaccactta tttaccggag ttgattatgt agaattctac
    11881 cgtcaaatac tagaaagcga aggagtcaat tgccaggttg tttttgctaa tattgcaact
    11941 agtattcttc cctatacaaa aaatgttctt aattgtgata ttcattcaag agataagaca
    12001 aaagaccttc tatgcaaggc aggagctaaa aaggtcttaa ctctggctga aatcctaaat
    12061 aaagcaaata ccagccatgg ctataatgaa gaatatggcc ttctgggagc caatgctgct
    12121 acagaaaaca ccattaaact ctttcccaga gactgccaaa gacttgttat ggatcttcaa
    12181 gcaagaatga aggaactaac aggcaagcta atagaggtta tggtctatgg agatggcgct
    12241 tttaaggacc ctgttggaca aatttgggaa ctagccgatc cagtcgtttc ccctggtttt
    12301 accccagggc ttagtggaac cccctcagaa gtaaaaatca aatatcttgc agataacacc
    12361 ttctcccacc ttaaaggcga tgatcttaaa ggtgccatca aaaaccatct agctaagatc
    12421 ttggaagaag atattagtca agccgagagc ctaggaacaa cacccagaag actaaccgat
    12481 cttattggca gcttaagcga tcttacttct ggtagtggtg acaagggaac tcccctggtc
    12541 tatatccaag gatattttga taagtttagt atcgactaat aaagaaaact tatgataaaa
    12601 gatagtacct tcttttaatt gccatactta cagaaaaaag aaagggtctt cgtcaaatga
    12661 tgttggttta gagaaatcga ctctatagac aagtacgaga gttatgtgta agggtgggct
    12721 aatccagtgt gaattagctc gcctttttat atttaaaggt gtcgtcacta atcggtatta
    12781 cttgtctgca aaggattaga tatctgtttt ta
//
LOCUS       DASRNA010000207         5781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_370796,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000207 DASRNA010000000
VERSION     DASRNA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370796"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1022
                     /locus_tag="VFD08_05840"
     CDS             192..1022
                     /locus_tag="VFD08_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK10574.1"
                     /translation="MNSIAVAIEQFARMIETIFEDKLIDSSLNRGQYAYLLYLFENEG
                     ANQYDISRDLFIDKTTVAKALKKLEETGYILRKTDQRDKRRVNVFLSKKGKGTYQEVK
                     NASDEIQKTISTNVHPDLVNKLESLLEIFIQELDIKWCEIKNYKRDVSFSVASIQDMN
                     LLKERQIYSFKPEEELFIERFGQYVINWIRYEQDESSEDVIGSELHFHQNSDVIHHGM
                     ELLREFEQWYFSNYSGKIQIRIPEGDISRQKLLHKNEYLFRSYDKQGDQAYYYYEKKK
                     "
     gene            1169..1696
                     /gene="cobU"
                     /locus_tag="VFD08_05845"
     CDS             1169..1696
                     /gene="cobU"
                     /locus_tag="VFD08_05845"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HZK10575.1"
                     /translation="MLELILGGARSGKSAYAEKKAENYSKVLYLATAQIKDDEMAHRV
                     ELHRQRRHKSWLTLEKYKCFQREDFTKADVVVIDCLTLMVTGLFFEKDRSHYNERDFA
                     LLEETIWHEIEHLLDLLKDKVVFLVSNEIGLGLVPEHKLSRVFRDMLGRFHQEIAHRA
                     DKVSFIVAGIPVEVK"
     gene            1693..2409
                     /gene="cobS"
                     /locus_tag="VFD08_05850"
     CDS             1693..2409
                     /gene="cobS"
                     /locus_tag="VFD08_05850"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZK10576.1"
                     /translation="MISAFLLQLSLYTTLYSPNHRWDEKEYRRGMVFIPLIGILLGLI
                     LYGTSLIIPDFLLGFSLLSLYCILSGGLHLDGFADTLDGVLARRGAKRALEIMKDPHL
                     GTFGVLGLFLLLLAYFNYLPLIDTSLIILPAIGRFGGMVAGLGNKYARSEGMGKFFIN
                     SIHWVHIVLWLFILFGLGYYLSGIQNVICLSLCFIYAFIGGRWFKKKLSGMTGDTVGF
                     IIESTQLLYIIFFLVVKQWI"
     gene            2400..2960
                     /locus_tag="VFD08_05855"
     CDS             2400..2960
                     /locus_tag="VFD08_05855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZK10577.1"
                     /translation="MDIILLRHTSSKDNELGVFSSPSTPLSSQGLEQVKELLDRKYPV
                     SKIYSSPYKRSLVLAEALSEKFNLPLILDKRLREIDFGDFEGKTFAQIQTLYPKEVEQ
                     WMEDPWAFVYPGGEDFHNVRSRVRRFYQELDESSLIISHQALMFNLMAEILEYDYKDL
                     SRFYLGSGAQVHLKSNPWRLLSLENL"
     gene            2969..3457
                     /locus_tag="VFD08_05860"
     CDS             2969..3457
                     /locus_tag="VFD08_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZK10578.1"
                     /translation="MKTKKLTIIALLIGLSILGSYITIFMSIALDSLPGFFAALSLGA
                     LSGGLVGGLGHFMTALIHGFPLGLPTHILVMFMMFLTCYFLGVFYKKSPLVGIIGAFS
                     INWLLTLFLSSLLSYLVGVTPGPLTLFPILLLPLFLGTLMNLLPAVLLHKILGERLNA
                     LS"
     gene            3444..4163
                     /locus_tag="VFD08_05865"
     CDS             3444..4163
                     /locus_tag="VFD08_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10579.1"
                     /translation="MRYRDLTILPLGDKKLIFATDSAGAIGRKKADLVKIDNRGLGRF
                     LAQVPFMEILATKALPSYVFLPICNEMEPTASEIIQGVMEIMDEAGLSRDFINGTTEE
                     NIPTIQTAASILVMAVVDIDFSFPKAFSGDTIFALGLPKVGQEVLDDRGDIMSLSHLK
                     KLRVFQEVGDILPVGSKGIAYEAKEMARSHGLDLHFLSDDPILSKSAGPGTVVLCSGK
                     AKIKKDLKDLALPLRKIAELL"
     gene            complement(4175..4687)
                     /locus_tag="VFD08_05870"
     CDS             complement(4175..4687)
                     /locus_tag="VFD08_05870"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF014694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HZK10580.1"
                     /translation="MLEVTSFEIDHIKLEPGLYLSWEQAYRDMVHMVLDLRLRRPNRQ
                     EILSDEESHSYEHAFAMSFRQANEFLGLEQEYFPLYFGPMGCKTGFYIIFSTPKKDME
                     LPLALLLKTTELIDDLEEVPARSPEACGHYQSLSLSAVKSVNKEVKEILNRLIKENKN
                     RDFYPGEKEA"
     gene            complement(4687..5700)
                     /locus_tag="VFD08_05875"
     CDS             complement(4687..5700)
                     /locus_tag="VFD08_05875"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005691152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HZK10581.1"
                     /translation="MKDLDWNDLGFKFRETNGMFVAHYKDGQWDEGSMLKDSYLTISV
                     GSTALHYGQQCFEGMKAYRAKDGRMLLFRPEENAKRMQESARRVLMAEVPKELFLKGV
                     KETVLTNQEYLPPYGSGGTLYLRPLLIGVGHNIGVSPAPEYIFCVFCMPVGSYFKGGM
                     HPVNFVTTDYDRAAPQGTGQAKVGGNYAGSLLPNKEATEQGYADCIYLDPLTHTKIEE
                     VGAANFFGITNKGEFITPKSPSILGSITKYSLLQVAEDLGLKSKEEDVFLDDLDHFLE
                     AGACGTAAVISPIGSITHKDKKTVFHSESEVGPITKKLYDRLTGIQFGDLEDLHGWVV
                     EVK"
BASE COUNT         1753 a         1048 c         1108 g         1872 t
ORIGIN      
        1 cggtttcata cgctaaataa gataatttaa attaaaaatt cgggctggtt ttttaggccc
       61 gtttttgttt gtaaaatatg taataattct tcttcttata taatttaata tacttgaaaa
      121 gagcgagtct ataagctaaa atgtaatata gaggaaagtt cacttgaata aaaactaggg
      181 aagggattac tatgaatagt atcgctgttg caatagagca gtttgctaga atgattgaaa
      241 caatatttga agacaagcta attgacagct ctttaaatcg tggtcaatat gcttatttgc
      301 tgtacctttt tgaaaatgaa ggggccaatc aatatgatat ttcaagagat ttatttattg
      361 ataaaaccac cgttgccaag gctcttaaaa agcttgagga aacaggctat attctaagaa
      421 aaaccgatca aagagacaaa agacgtgtta atgtcttttt gtccaaaaaa ggaaaaggaa
      481 cctatcagga ggtcaaaaac gcttccgatg aaatccagaa gacaattagc acaaatgttc
      541 atcctgatct tgttaataaa ctggaatcct tacttgaaat ttttatacaa gaattggaca
      601 taaagtggtg tgagataaaa aactacaaaa gagatgtttc tttttccgtt gcaagtatac
      661 aagatatgaa tctgctcaaa gaacggcaaa tttatagttt taaaccagaa gaagagctct
      721 ttattgaacg ttttggacaa tatgttatta actggattcg ctatgaacaa gatgaatcta
      781 gcgaagatgt aattggaagt gaattgcatt ttcatcaaaa ttctgatgtt atccaccatg
      841 gtatggaact ccttcgagaa tttgaacaat ggtatttttc aaattatagt ggaaaaatcc
      901 aaatcagaat ccctgaaggg gatatttcaa gacaaaaact actacataaa aatgaatacc
      961 tctttcgctc ctatgacaaa caaggggacc aggcttacta ttattatgag aagaaaaaat
     1021 aaactgctct tctgagcagt atttttcagt agaaaacttc ctctatgtga actttctaca
     1081 aactttaaat tctagtgatt tgctggaagg aaaaaacgct gctacataga tttcattaaa
     1141 tttactaact taagataggg gactaatcat gctagagtta attttagggg gggccagaag
     1201 cggaaaaagc gcttatgctg aaaagaaagc tgaaaactat tccaaggttc tttatctagc
     1261 tacagctcag attaaagatg atgagatggc tcacagagtt gagcttcatc gccaaagaag
     1321 acataaaagc tggctaactc ttgaaaaata taaatgcttt caaagagaag atttcacaaa
     1381 agcagatgtt gttgtaattg attgtttaac tcttatggtt actggccttt tttttgaaaa
     1441 ggataggagc cattataatg agagggattt tgcactacta gaagaaacta tatggcatga
     1501 aattgaacac cttctagatt tgctaaagga taaggttgtc tttcttgtat ccaatgaaat
     1561 tgggcttgga cttgttcctg aacataaact ctcaagggtc tttcgagata tgctaggacg
     1621 ttttcaccaa gaaattgccc atagagccga taaagttagc tttattgttg ctggaattcc
     1681 tgtggaggtc aaatgatttc tgccttctta ttgcaactaa gtctttatac aaccttatat
     1741 agtcccaatc acagatggga tgaaaaagaa tacaggcgtg gaatggtttt tatcccacta
     1801 atcggtatcc ttttgggtct tattctctat ggcacttcac ttataatacc agatttcttg
     1861 ctgggttttt cgctgttaag tctttattgt attttaagtg gtggcctgca tttggatgga
     1921 tttgctgaca ccttagatgg tgttcttgcc cgtagggggg ctaaaagagc tctggaaatt
     1981 atgaaagacc cccacttggg tacctttggt gtattaggtc tttttttgct tcttctagcc
     2041 tattttaatt acctgccact aattgatacc agtctaatta ttttacctgc tataggtcga
     2101 tttgggggca tggtggccgg acttgggaat aaatatgcca gaagcgaagg gatggggaag
     2161 ttttttatta attccatcca ttgggttcat attgttttat ggctatttat cctatttgga
     2221 ttaggctatt acttaagtgg tatacaaaat gttatttgtc tgagtctttg ctttatctat
     2281 gcttttattg gcggaagatg gtttaagaaa aaactttctg gcatgacagg agacactgta
     2341 ggctttatta ttgaaagcac ccagctctta tatataattt ttttcctggt ggtcaagcaa
     2401 tggatataat acttttaaga cacacatcga gtaaagacaa tgaattagga gttttttcta
     2461 gcccttctac ccctctaagt tcccaaggct tagaacaagt aaaagagctt ttagaccgga
     2521 aatatcccgt atcaaaaatt tattcttctc cttataagcg aagcctagtc ttggctgagg
     2581 ccctatcaga aaagttcaat ctgcccttaa ttcttgataa acgtctaagg gagatagatt
     2641 ttggtgattt tgaaggaaaa acttttgccc agattcagac cctttatcca aaagaagttg
     2701 agcagtggat ggaagaccct tgggcttttg tttatccagg tggggaagat ttccataatg
     2761 ttcgaagtag ggtccgaaga ttttaccagg aactggatga atctagtctt attattagcc
     2821 accaggcact catgtttaat cttatggctg aaattctaga atacgactat aaggacctta
     2881 gccgttttta tttgggcagt ggggcccaag tacatcttaa aagcaatcct tggagattgc
     2941 tttctcttga aaacttatag gaaagataat gaaaacaaaa aaacttacaa ttattgcact
     3001 tttaataggc ctaagcattt taggcagcta tattaccata tttatgagta tagcactgga
     3061 ttcactgcct ggtttttttg ccgctcttag cctaggcgct ctttctggag gccttgttgg
     3121 aggtctaggt cattttatga ccgcccttat tcacggtttt cctctgggac ttcctactca
     3181 tattttagtt atgtttatga tgtttttaac ctgttatttt ttaggtgtat tttataaaaa
     3241 gagtcccctt gttggaatca taggagcctt ttctattaac tggctattaa ctttattttt
     3301 aagttcttta cttagttatc tagtaggggt cacaccaggt ccattaacac tttttcccat
     3361 acttcttctt ccattgtttc ttggaacttt aatgaatctt ctaccagctg tcttactcca
     3421 taaaatccta ggggaacgtt taaatgcgtt atcgtgacct tacaattctt cctctaggag
     3481 ataaaaaact tattttcgcc acagatagtg ccggagccat cgggaggaaa aaagcagacc
     3541 tggtaaaaat agataatagg ggcttgggca gatttcttgc tcaggttccc tttatggaaa
     3601 tcctggccac caaggccctt ccttcctatg tctttcttcc tatttgcaat gaaatggaac
     3661 ccacggcttc agaaatcatt caaggggtta tggaaatcat ggatgaggct ggtctgagtc
     3721 gcgacttcat caatggaacg accgaagaaa atatccctac aatccaaact gcagcctcta
     3781 tacttgttat ggcagttgtt gatatagatt tttcttttcc caaggctttt tctggtgaca
     3841 ctatctttgc ccttggttta cccaaggttg gccaggaagt gttagatgat aggggagata
     3901 ttatgagtct aagccatctt aaaaaacttc gggtctttca agaagtagga gatatactac
     3961 cagtaggaag taagggtata gcctatgaag caaaagaaat ggccaggagt catggattag
     4021 acttacactt tctatcagat gatcccattt tatctaagtc ggctggtcct ggaacagtgg
     4081 tactatgtag tgggaaagca aaaataaaaa aggaccttaa agatttggct ttacccctaa
     4141 gaaaaattgc ggaattatta taaaaaaaag gaggctatgc ctccttctcg ccgggataaa
     4201 aatcccgatt tttattttcc ttgattaatc tatttaggat ttcttttact tctttattta
     4261 ctgatttaac tgctgataag cttagacttt gataatgacc acaggcttca ggggagcgag
     4321 cgggtacctc ctctaaatca tcaattaact cggttgtttt taagagtaga gctaagggca
     4381 attccatgtc cttttttggt gttgaaaata taatataaaa acctgtttta caacccatag
     4441 gaccaaagta caagggaaaa tactcctgct caagtccaag gaattcattt gcttgtctaa
     4501 agctcatagc aaaagcatgt tcataggaat gactttcttc gtcgctgagt atttcttgac
     4561 gattgggacg gcgaagccta agatctagta ccatatgaac catatcacga taggcctgtt
     4621 cccaggaaag atatagacca ggctcaagct taatatgatc tatctcaaaa gaggtaactt
     4681 ctagcattat tttacctcca caacccagcc gtgaagatct tctagatctc caaattgaat
     4741 gcccgttaat cgatcataga gttttttggt gattgggcca acttcacttt cactgtggaa
     4801 tactgttttc ttatccttat gggttataga tccaatagga gagataacag ctgcggttcc
     4861 acaagcacca gcttctagga agtgatccaa gtcatcaaga aatacatcct cttctttact
     4921 ctttaaacca agatcttccg caacttgtaa aagagaatat tttgttatac tacctaaaat
     4981 agagggggat ttcggtgtta taaattctcc cttatttgta attccaaaaa agtttgctgc
     5041 tccaacttct tcaatttttg tatgagttag ggggtctagg taaatgcaat cagcataccc
     5101 ttgctctgta gcttccttat ttggaagaag gcttccagca tagtttcccc ctaccttggc
     5161 ttgtccagtc ccttgggggg cagctcgatc ataatctgta gttacaaagt ttaccggatg
     5221 cattccacct ttaaaatatg agcctacagg catacaaaaa acacagaata tatattctgg
     5281 ggcaggactt acacctatat tatggcccac accaataagt aaaggtctta aatataaggt
     5341 ccctccgcta ccatagggtg gaagatactc ttggttggtt aaaacggttt ctttaacgcc
     5401 tttaagaaag agctctttag gaacttcagc cattagaact cttctagcac tttcttgcat
     5461 tctttttgca ttttcttctg gtctaaagag tagcatccta ccgtcctttg ctctataggc
     5521 cttcattcct tcaaaacact gctggccata atggagggca gttgaaccaa cacttatggt
     5581 taagtagcta tccttgagca tggaaccttc atcccactgt ccatccttat aatgtgcaac
     5641 aaacatgcca ttagtttctc taaatttaaa gcctaaatca ttccaatcta aatctttcat
     5701 aatatgctcc tttcattgtt aataataagt ataacacttt aaaaaggtat ttaataatta
     5761 ttccttttaa aatttaaaac t
//
LOCUS       DASRNA010000208         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_71879,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000208 DASRNA010000000
VERSION     DASRNA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71879"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFD08_05880"
     CDS             complement(<1..754)
                     /locus_tag="VFD08_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HZK10582.1"
                     /translation="MKKGEIYTCTITKNKYPATGICYLENEEIAIPGAFLDEEVTFRL
                     GKRKKGLRTGRLLQRQEAPCDQFDRCGGCISQHLDLEKQRKIKLMEVRDLFKRQGVIL
                     EDLTISGSEQALYYRNKVEFNFGDTTKGGPLELGFYERNMGRNVLPTKDCLLVHRDIR
                     IIRSEILAFSKENDYSHYHVMKREGFLRNLVVRRSHYTGEIMVNLVTTTQETLKHDFI
                     KRLLELDLEGEIVSILHTENDSLSNFVYSDKLH"
     gene            831..1529
                     /locus_tag="VFD08_05885"
     CDS             831..1529
                     /locus_tag="VFD08_05885"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HZK10583.1"
                     /translation="MRKKLGIFFIVLAVILFALYFGTGTILKLISNPEAIEELTVEEI
                     EENKERQAVFQWDAITAIDSVSVITSAKPINKEAIIAQMIVPELEINLPVLRGITTEN
                     LNHGVATLYDSASLGEGNFSLAGHNMSSYNVLLNRFYDMPDRPFDIYFHDKKTVYHYV
                     VYAKLYTDQYAFYLTEDAEADRRGKPIVSILTCDRPYEEDQRIFIMAELIDTYAYTSP
                     LELRPSEQTKAYRN"
     gene            complement(1552..>2023)
                     /locus_tag="VFD08_05890"
     CDS             complement(1552..>2023)
                     /locus_tag="VFD08_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZK10584.1"
                     /translation="YGEDPEPEAGPYDIKRDKFEINLVHQAQKRKIPILGIGRGMQII
                     NVALGGSLNQSIVSKILHHRPRGTKKDPLHRIQILPGRLYEVFGEELIVNSYHSQSIK
                     TLGKGLEVSARSIDGVIEAIEGPGIMGVQFQPELFEHNDRFILLFKSFVENLEK"
BASE COUNT          533 a          431 c          384 g          675 t
ORIGIN      
        1 gatgtaattt atctgaataa acaaaattcg aaagagaatc attttctgta tggagaattg
       61 aaacaatttc tccttccaag tccaattcaa gaagtctttt tataaaatca tgctttaggg
      121 tctcttgtgt tgtggtgaca agatttacca taatctcgcc tgtgtagtgg cttcttctta
      181 cgaccaggtt acggagaaag ccttctcttt tcattacatg ataatggcta tagtcatttt
      241 ctttggaaaa agcaaggatc tcacttcgaa ttattctaat gtccctatgc acaagtaagc
      301 agtcttttgt gggaaggaca tttcttccca tattgcgctc ataaaagccc agctctagtg
      361 gtcccccctt tgtggtatca ccaaagttaa attctacctt gtttcggtag tagagggcct
      421 gctcactccc ggaaatggta aggtcttcta atataacacc ctgcctttta aataaatctc
      481 tgacctccat aagcttgatt tttctttgct tttctagatc aagatgctgg cttatgcagc
      541 ctccacatcg atcaaactga tcacaaggcg cttcttgtcg ctgcagaagt cttcctgtgc
      601 gcaagccttt tttgcgtttt cccagacgaa aagtaacctc ttcatcaaga aaagcgccag
      661 gtattgctat ctcttcgttt tcaagatagc aaattcctgt cgcaggatac ttgttcttag
      721 tgattgtaca tgtataaatt tcgccttttt tcattgttcc tcactgttat ttctttagcc
      781 tttattgtag tcctcctcaa tctttgctat acttttacta agggggaaat atgagaaaaa
      841 aactaggtat cttttttatt gttcttgctg ttattttgtt tgccctatac tttgggacgg
      901 gaaccattct taagttgatt tctaatccag aagccataga ggagctcact gtagaagaga
      961 tagaagaaaa caaggagcgt caagccgtct ttcagtggga cgctattacc gctattgata
     1021 gtgtttctgt tattacttca gctaaaccca tcaacaaaga agcaattatt gcccagatga
     1081 ttgtccctga gctggaaatt aatttgcctg tgcttcgagg catcacaacc gaaaacctca
     1141 accacggtgt agccacgctc tatgactcag ctagtctagg ggaaggcaat ttttctcttg
     1201 caggccacaa tatgtctagc tacaatgttc tcttaaaccg tttttatgat atgcctgatc
     1261 gcccctttga tatttatttt catgataaga aaacagtata tcattatgtg gtttatgcta
     1321 agctttatac tgaccagtat gctttttatc taactgaaga cgcagaagct gatcgccggg
     1381 gaaaacccat tgtcagtatt ctcacctgtg atagacccta tgaggaagat caaaggattt
     1441 ttattatggc tgagctaatt gacacctatg cctacacaag tcctctggag cttaggccca
     1501 gtgagcaaac aaaggcctat agaaattgag aagtcctagg acttctcttt tttatttctc
     1561 taagttttcc acaaagctct taaacaaaag gataaagcga tcattgtgtt caaataattc
     1621 tggttgaaat tgtactccca taatacctgg gccctcaatg gcttctatga ctccatctat
     1681 acttcttgca ctgacttcta gtcccttacc cagggttttt atactctgtg agtggtagga
     1741 gtttacaata agttcttctc caaaaacttc atagagccgg cctggtaaaa tttgaatccg
     1801 gtgcaaggga tctttttttg ttcccctagg tctgtgatgg aggatttttg atacaatgct
     1861 ttggttgagg ctccctccta gggcaacatt gataatttgc atacctcggc cgatgccaag
     1921 aatgggaatc tttctttttt gtgcttgatg gacaagattt atttcgaatt tatctctttt
     1981 tatatcatag ggccccgcct cgggctcggg atcctcccca tag
//
LOCUS       DASRNA010000209         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_665629,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000209 DASRNA010000000
VERSION     DASRNA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665629"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..759
                     /locus_tag="VFD08_05895"
     CDS             187..759
                     /locus_tag="VFD08_05895"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZK10585.1"
                     /translation="MRKAWDGITSSLVVFMLIILMALLGARLLGLEVFTVLSASMEPE
                     YQTGSIIYVKEVDYRNLKRGDVITFSMGDKSVVTHRIVEALVDKKDPTMIYYRTKGDA
                     NNSDDGGLVHYKDIIGKPVFAIPKLGFFVNYIQNPPGIYLALFTGGLLIFFTLLAEAF
                     LGLVKKATASRQIFQKKRENKKKERGHNFS"
     gene            816..1631
                     /locus_tag="VFD08_05900"
     CDS             816..1631
                     /locus_tag="VFD08_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HZK10586.1"
                     /translation="MTRKHKTLLLSMAAVVLLAIGVMGTFAYLTSQSETVTNTFTVGR
                     VSFSKDQYQKTKGLNETEVDQYGIPKYRYSDGLFLINKADGLLDAGATPITVDGKNVY
                     IDSEGNFVDRYHNPLTVEGITTIENYAQENGGEALRVTANEYKLLPGKKYLKDPKAFI
                     ERGSEPAYVFVELTNPLESIEANEAASTIQAQVESNGWIQISKEAEKSLWRHKDIVDA
                     SESEIELAIFESFTVDKQAQIDIAPGDIVVKAFAIQADGLSPENALEQVNFAH"
     gene            1826..2389
                     /locus_tag="VFD08_05905"
     CDS             1826..2389
                     /locus_tag="VFD08_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10587.1"
                     /translation="MKRRLLAGLLIIICLLIQIHETKAYFSTEKTLHKVITTGGIKIE
                     LLEWEDESKIRSLPKNSTEGVMPGREIVKIFQVENTGKNEAYIRIQMEKDIGLKGKIG
                     EEPNLNLMIVNLESSAWTLAEEGFYYYKKVLKPGEKTEPFYLTARFDRSMGNIFQDSR
                     VKVSITAQATQVANNGNSSLKAKGWPD"
     gene            2433..>2663
                     /locus_tag="VFD08_05910"
     CDS             2433..>2663
                     /locus_tag="VFD08_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10588.1"
                     /translation="MKNKYRGSLLLFLLLFFMGLGFCLPAFAQESPLLFKKEKVIIFS
                     SRTIYAANDLFSNFKDLMPGDVKKEELVLENKS"
BASE COUNT          963 a          419 c          569 g          712 t
ORIGIN      
        1 gtttgagacc tatagtaaat aaattcctgt tactagttgg aaatgtgtat caaaagacaa
       61 gatagctagg acccaaggtg atattatgat gcccaaagga agaagattct ttgataaagg
      121 ccccacttgg cttgaaaagt caggcaacag tgactcctac ttatgagtag gtaaaaggga
      181 aaagaagtga gaaaagcttg ggatgggatt acaagtagtt tggttgtttt tatgcttatt
      241 attcttatgg cacttctagg agccaggctt cttggtctag aagtttttac cgttctttca
      301 gcctccatgg aaccagagta tcaaacagga agcatcatct atgtaaaaga ggtggattat
      361 aggaatctta aaagagggga tgtgatcacc tttagcatgg gtgataagag tgtggttacc
      421 caccgaattg ttgaggctct tgtagataag aaagatccta caatgattta ttaccgcacc
      481 aagggtgatg caaataactc tgatgatggg ggacttgttc actataaaga cattattgga
      541 aaacctgtat ttgccatacc gaaactaggt ttttttgtaa attatataca aaacccacca
      601 gggatttacc tggctctttt tacgggaggt cttctaattt tctttaccct attagctgag
      661 gcttttctag ggctggtaaa aaaggcgact gccagtagac agatttttca aaaaaaaaga
      721 gaaaacaaaa agaaagaaag agggcacaac tttagttaaa aaatcagaaa gatcagagaa
      781 acgagccata aaaggcttat ttaggaggag aaaacatgac aagaaaacac aaaactctac
      841 tactatcaat ggccgcagta gtactactag ccataggtgt tatggggaca tttgcttact
      901 taacgtcaca atcagaaacg gtaacaaaca catttacagt tggtagagtt tcatttagca
      961 aggatcaata tcaaaaaaca aagggtctaa atgaaaccga agtggaccag tatggaattc
     1021 caaagtatag gtacagtgat ggactatttt taataaacaa ggcagatggt ttacttgatg
     1081 ctggggctac gccaattact gttgatggta aaaatgttta tattgattca gaaggcaatt
     1141 ttgttgatag ataccataat cctcttactg tagagggaat taccaccatt gaaaattatg
     1201 cccaagaaaa tggaggtgag gcccttcgtg taacggcaaa tgaatataag ttattaccag
     1261 gaaaaaaata tctaaaagat cccaaggcct ttatagaaag agggagtgaa cctgcctatg
     1321 tatttgttga actaacaaat cctttggaat ccattgaagc aaatgaggcc gcatcaacaa
     1381 tccaagctca ggtagaatca aatggctgga ttcaaatatc aaaagaagca gaaaaatcgc
     1441 tctggcgtca taaggatatt gtagatgcta gcgagagtga aatagaacta gcaatttttg
     1501 aaagttttac cgtggacaag caagcgcaga ttgatatagc accaggagat attgtggtaa
     1561 aagcatttgc cattcaagca gatggattaa gtccagaaaa tgccttagaa caagtaaact
     1621 ttgctcacta aggaaaatct tgtaaaaatc agtggatatc ttacaggtgg atatccactt
     1681 atggataaaa aagaaagtag aaaaaatggt gtcaaaatct cacccccaca cttgcctagg
     1741 catattttgc aattaagact gggcttgata aaaacaagta attataaata aaagctaatt
     1801 gatctaagtc aagtggggtg aaaggatgaa gcgaagactt cttgctgggt tactaattat
     1861 aatttgtctt ctaatacaaa tacatgaaac aaaggcatat ttttctaccg aaaaaaccct
     1921 gcataaggtg attacaacag gggggataaa gattgagctt ctagagtggg aggatgaaag
     1981 taagattagg tctcttccca aaaatagtac agaaggagta atgccgggaa gagaaatagt
     2041 aaaaatcttt caagtagaaa acacgggtaa aaatgaggct tatatacgga ttcaaatgga
     2101 aaaagatata ggattaaaag gaaaaatagg ggaagagcct aacttaaatc ttatgatagt
     2161 aaaccttgaa agctctgctt ggacacttgc tgaagaggga ttttattact ataaaaaagt
     2221 ccttaaacca ggagaaaaga cagaaccatt ctatcttaca gcaagatttg atcggagtat
     2281 gggtaatatc tttcaagata gcagggtaaa agtaagcatt actgcccaag caacccaggt
     2341 tgccaataac gggaatagtt ctcttaaagc caagggctgg ccagactaaa aaccaaaaca
     2401 agtattctta tcagttagct agggggcggg ggatgaaaaa caaatataga ggcagcctac
     2461 tcttattcct actgcttttt tttatgggtc ttggtttttg tcttcctgca tttgcccagg
     2521 aaagccctct tttatttaaa aaagagaagg ttataatctt ttcttcaaga acaatctatg
     2581 ctgcaaatga tctttttagt aactttaaag acttaatgcc aggagatgta aaaaaagaag
     2641 aacttgtcct agaaaataaa agt
//
LOCUS       DASRNA010000210         8098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_73455,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000210 DASRNA010000000
VERSION     DASRNA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73455"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFD08_05915"
     CDS             complement(<1..544)
                     /locus_tag="VFD08_05915"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZK10589.1"
                     /translation="MDQLRIENIKELPRIKPSIYEELPSTNSLLKELAKDGAQEGRLI
                     LAQGQSAGRGRKGKSFFSPKNTGLYMSLLLRPKAGVTVELLTAATAVAISRAVKDTLG
                     LELAIKWVNDIYYKGKKVGGILTEGSFSRGPCPDYIIVGLGLNLYQPEGGFPQELTLA
                     GALLDKKQEGLLNLLVKQILE"
     gene            851..3190
                     /locus_tag="VFD08_05920"
     CDS             851..3190
                     /locus_tag="VFD08_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK10590.1"
                     /translation="MKETRLSNEPQKFINTKLDELRQIYVNITTSYEFFYVIEGTIYA
                     NCYEETHRLESGDMFMATPGQAFMLIPDSEPALYISMSIHPLGVKKQELYHQKILPCV
                     FKKDQIRQAHLNLLYSIYQESFQAEHSQVQELIEVFLQKLPWKERQTGLETQSDKKME
                     LILKASQILADVDPGLEELALKLNISPSYLSRVFKEVTGMRFSDFSQRKKLQKSSQLL
                     LSGMSIEDIALTIGFTSSKSLNRIYRQFVKTTPSSYRKYLLELANNQKKEEDLRYKAS
                     FQKFVEHYAEPSYTNTMKEYLGEKYNYHHVSTSAPSIQDPTLGQRAVSLDNFGEDYLK
                     EIQSLNSINSNGQLALNLDIPSEDNGAIFFKDLNKWGDEEDLFKILAKINEINIPAIL
                     SVGVKDQSIKEFCKNHKREEIGDYLKLVENFYKKIIKNVTRPIAETYYYVFDTSGVLE
                     FESPKSIMYFFEFVEKHHLMLEQLLYTSNFNSVYHLGKQSSAELEDIVDFISKNRHRV
                     RLPKYSYAQITLAQKLKLSNPHQLLSAIEQSKKVIKQLNAFEEDDRLKRTKIKLQGID
                     MDLVIDYVDPLYHDLFFAILTLDLWMKLKGKSRIIPNYNFSFACRTKESLFTSKLIDK
                     SGFPTPFYHINLFVSQMPENVIYHKEGCLISKNEDEIHLLVYTNPLLDYSFAIEKGFE
                     YLSDYKREVFIDISDLKGYYKETTQILNYENGSPYFQLRNFKQPELMSAYEKEYVRSH
                     STPLLMSDYKEVSGKLELTLTLKPFEVVYKVFTKIKDPR"
     gene            complement(3212..4015)
                     /locus_tag="VFD08_05925"
     CDS             complement(3212..4015)
                     /locus_tag="VFD08_05925"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZK10591.1"
                     /translation="MKILLCDLDGTLLTSKKTITPRVQKAVDLWQSKGNLFVIATGRL
                     ISSAQLFADNSGTGDLVVACSGATIYKDNRLIYEKTVPLHLVERLWEILKPQEEYCQV
                     YCDRSLVYNRDRGIFGSYVAHKDYDAKYRLPLIHMEDFDKEILPGPVHKLSFICHDPK
                     RAKEIICQMGDLSEVNVFRSLPHLYDIIYKEADKGVAGLWLKDKLRAKEFYAIGDNEN
                     DVAMLEQAKISAVMKDAPRSVAKKADLTVSTSDKDGVAEFITYLLDKDL"
     gene            complement(4012..6075)
                     /gene="rnr"
                     /locus_tag="VFD08_05930"
     CDS             complement(4012..6075)
                     /gene="rnr"
                     /locus_tag="VFD08_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZK10592.1"
                     /translation="MQILELLKSKNRSQSLEEIQRALKIEDVKELLLELEKLENKGQI
                     RKTRKGRYFPHKGKSLLAGKMSIPGSSYGFFVYDEGSKEDVFVHSSNFSNAMDGDEVL
                     IKLIPSNRKARKEGKVVKITKRKHQSVMGIYHSKGFVIPLNRRLAFSVFVDKKNRNKA
                     IDGEIVQVEIIDYGSRNTKLRGKVIQKLGELYSPGLDFKLVVEEFDLPVHFPKEVIRE
                     AKRFSSPSQEEISQRKRVEDLVFTIDGPDAKDFDDAISIKRHKGGYRLGVHIADVSHY
                     VKEGSYLDREAYKRASSVYLVDRVIPMLPEEISNELCSLNPNEDKLCLSVVMDIDSKG
                     KVLSSDIFPSVLASSYRLIYDDVTEFLEGKENKELEPVRKELECFEDLSKILIKNRDD
                     RGGIDFGSNEPYFVMDDEGWPIEVHRREQGLADKMIEEAMILTNKTVSEHFSWMEVPF
                     LYRSHEKPDGEKMAELNNLLHSFGYLIKGDVEEIHPKQLSELLKSLKGKPEEALLVSK
                     ILRYLKQARYTDDLIGHFGLALTHYSHFTAPIRRYPDLQIHRIIKEAILGISKSQREH
                     YKAILPEVAQHSSEAERKADEAERRMDEIKKAEYMQDKVGQEFEGKVSGVTNFGIFVE
                     LDNTIEGMIRLEDLKDYYELDVDKTKLVGKKGKEIQLGQPLKLILTSVQVYQGEINFI
                     PKEEL"
     gene            complement(6096..6473)
                     /locus_tag="VFD08_05935"
     CDS             complement(6096..6473)
                     /locus_tag="VFD08_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZK10593.1"
                     /translation="MSIKSKYGSEKWIDNGRGKIPLGSGEGQYLPYDLLYGALSACYY
                     YTLESLCEKMKIDYEEVTITIDGKKKEAKIATLERVDFVIEAKGVTEEKKFTKACSLA
                     GKYCSIHATVAKVAKMNHEIIFL"
     gene            complement(6483..6884)
                     /locus_tag="VFD08_05940"
     CDS             complement(6483..6884)
                     /locus_tag="VFD08_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10594.1"
                     /translation="MRVLLLIFLLLMSSCSQEKPEEMCLGVVEDYYESLITQDLQRQL
                     EILAPFSLDAKSYNEGFLSHVLGGEVHEITIEHEDELLIMVKLDFTLILDESFKENVS
                     FQPGENHVQRYFSFFKNDDYRLKEILNKAIY"
     gene            complement(7125..7460)
                     /gene="yedF"
                     /locus_tag="VFD08_05945"
     CDS             complement(7125..7460)
                     /gene="yedF"
                     /locus_tag="VFD08_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HZK10595.1"
                     /translation="MKEVIIINKDTMGSGSQELGKRLIIAFVKKLWASPIKPEAIIFY
                     NGAVKLLTSAGGCLEALHFLEEAGVDLVACGSCVNFFDLNKQLRVGRISSMEEILDMI
                     QKAQKVVTI"
     gene            complement(7501..>8098)
                     /locus_tag="VFD08_05950"
     CDS             complement(7501..>8098)
                     /locus_tag="VFD08_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10596.1"
                     /translation="LDYIYRRLVTSDFLKIYDELPNFVEGIKSAKTVLVGPIASQIIH
                     NKMLFFVLHHPGAREHFTLEQIKFIENHIPFTALIDEKVASDAHFVKEKNKYLLKPTD
                     SYASRGIYLGSDMQEGEWKATLEKLVGKEYLIQAFSDLPANKILSFEEGVKSFNHMTG
                     LFVYNGKLKGFFSRACQGPIIASSYGGKSLASLGIKKL"
BASE COUNT         2375 a         1808 c         1327 g         2588 t
ORIGIN      
        1 cttccaatat ttgctttaca agaaggttta acaagccttc ttgtttttta tccagcaagg
       61 ctcctgctag agtcagttct tgtggaaatc ctccctcggg ctggtaaaga ttaaggccaa
      121 ggcccactat tatataatca gggcaaggac cccttgaaaa agatccttct gttaggattc
      181 ctcccacctt ttttccctta taataaatat catttaccca tttgatcgca agctccagac
      241 ccaaagtatc tttaactgca cgacttatgg ccactgctgt agccgctgtt aaaagctcta
      301 ctgttacgcc tgccttaggc cggagcaaaa gactcatata aagaccggtg tttttgggag
      361 aaaaaaagct ttttcctttt cgccccctcc ctgcactctg gccctgggcc aagattagtc
      421 ttccttcctg cgctccatcc ttggctagtt cttttaataa actatttgtt gatggcaatt
      481 cttcataaat actcggcttg attcttggca gttcttttat attttctatc cttagctggt
      541 ccattgtttt gcctccttta gtcattgtaa taaaaagata aaaacaaatc cagtaagatt
      601 gctattcttt ttacagtatt tgatgtattt gttcacaacc ggccaatatt tcacaattat
      661 aatgttattt tttttgcttt ccctcaatgc attgacaagt gtttttttac agattatgat
      721 acttatggct agattggata gtttctactt tttttggaaa ataccatctt gcagataaca
      781 aatagttcat acttacccta tatatttgtt taattcaaag aaccactcgc aacttaccca
      841 aggaggcgaa atgaaagaaa ccagactctc caatgaaccc cagaagttca taaacacaaa
      901 actcgatgag cttagacaaa tttacgtcaa tattactact tcctatgagt ttttctatgt
      961 aattgaagga accatttatg ccaattgcta cgaagaaacc catcgtttag aatccggtga
     1021 tatgtttatg gcaactcctg gacaagcctt tatgcttatt cctgatagtg agccagcctt
     1081 atatataagt atgagcattc atcccctagg agtaaagaag caagaactct atcaccaaaa
     1141 aatacttccc tgtgtcttta aaaaggacca gattcgtcaa gcccacctca atttactata
     1201 tagcatttat caggaaagct ttcaggctga gcatagtcaa gtccaagaac ttattgaagt
     1261 ttttttacaa aagttacctt ggaaagaaag acaaaccggc cttgaaacac aatctgataa
     1321 aaaaatggaa cttattttaa aagcttccca gattcttgca gatgtggatc cagggcttga
     1381 ggaactagcc ctaaagctca acataagtcc ctcttatcta tccagagttt ttaaagaagt
     1441 aactggtatg cggttttctg atttttctca aaggaaaaaa cttcaaaaat cctcccaact
     1501 acttctaagt ggtatgagta ttgaagatat tgccctaaca ataggcttta cttctagcaa
     1561 gtccctaaac cgcatctatc ggcaatttgt taaaaccaca ccttcttcct atagaaaata
     1621 tctattagaa ctggcaaaca atcaaaaaaa agaagaggac ctccgttata aggcttcctt
     1681 ccaaaaattt gtagagcact atgctgaacc aagctataca aatactatga aggagtacct
     1741 aggagagaag tacaactacc accatgtatc tacctcagcc cctagtatcc aagatccaac
     1801 cctaggccaa cgagctgttt ccctagataa ttttggtgag gactatctta aggaaataca
     1861 gtctttaaac agtatcaatt ccaatggcca attggcccta aatctggata tcccctcaga
     1921 agataatggg gctattttct ttaaagacct aaataagtgg ggcgatgagg aagatctctt
     1981 taagattttg gctaaaatta acgaaatcaa tatacctgct atcttatcag ttggggtaaa
     2041 agatcagtct atcaaagagt tttgtaaaaa ccacaaaaga gaagaaatag gcgattatct
     2101 taaactagta gaaaattttt ataaaaaaat aataaaaaat gttaccaggc ctatagctga
     2161 aacttattac tatgtatttg atacctccgg agttttagaa tttgaaagcc caaaatccat
     2221 tatgtatttt tttgagtttg tagaaaagca tcatcttatg ttggagcagc tactctatac
     2281 ttcaaacttc aatagcgttt atcatcttgg aaagcaaagt tctgccgaac tggaagatat
     2341 agtcgatttc atctctaaaa accgtcaccg agtacgtctt ccaaaataca gctatgcaca
     2401 aattaccctt gcccaaaagc taaaactatc caaccctcat caacttctaa gcgccataga
     2461 acagtccaaa aaagttatca aacagctaaa tgcctttgaa gaagatgatc gcttaaaacg
     2521 aacaaaaata aagctgcagg gtatcgatat ggacctagtt attgattatg ttgatccgct
     2581 ctaccatgac ttgttttttg ctatcctaac tcttgatctt tggatgaagc taaaggggaa
     2641 atctcgcatt atccccaact ataatttcag ctttgcctgc agaacaaaag aaagcctatt
     2701 tacaagcaag ttgattgaca aaagtggttt tcctacccct ttttaccata taaacttatt
     2761 tgtatctcaa atgcctgaaa atgttattta tcacaaagaa ggttgcctaa taagtaaaaa
     2821 tgaagatgaa atacatctgc tagtttatac caatccccta ctggactact cctttgccat
     2881 tgaaaagggt tttgaatacc tgtctgatta taaaagagaa gtctttatag atattagcga
     2941 tctaaaggga tattataaag agacaacaca gatcttaaac tatgaaaatg gttcgcccta
     3001 tttccagctt cggaatttta agcagcctga acttatgagt gcttacgaga aagagtatgt
     3061 gcgctcacat agcactcccc ttcttatgag tgactacaag gaagttagcg gtaagctaga
     3121 attaacccta acactcaagc cctttgaagt tgtctacaaa gtctttacaa aaatcaagga
     3181 ccctcgctag ggtccttttt tccactccag attaaagatc tttatccaga agataagtga
     3241 taaattcagc taccccatct ttatcactag tagagacagt aaggtcagct ttttttgcca
     3301 cgctccttgg agcatccttc attacagcag atatctttgc ctgctctaac atggctacat
     3361 cattttcatt atccccaatg gcgtaaaact ctttggccct tagcttgtcc tttagccaaa
     3421 gcccggccac ccccttatcc gcttccttat agataatgtc atacaaatgg ggcaaggagc
     3481 gaaaaacatt gacctcagac aagtcgccca tctggcatat tatttccttg gctcgctttg
     3541 gatcgtgaca gataaaactc agtttatgca caggaccagg taggatctcc ttatcaaaat
     3601 cttccatatg gataaggggc agtctatact tggcatcata gtccttatgg gccacatagg
     3661 atccaaatat tcccctatcc ctattgtaga ccaggctccg atcacaatat acctggcaat
     3721 actcttcctg gggcttgaga atctcccaga gcctttcaac taggtgaagg ggtacagttt
     3781 tttcataaat taacctattg tctttataga ttgttgcacc actacaggct acaacaagat
     3841 ctcctgttcc actattatcg gcaaaaagct gagctgaact aataagtctt cctgtagcta
     3901 taacaaaaag atttcccttg ctttgccata aatcaacggc tttttgtact cttggggtaa
     3961 ttgttttttt acttgttaag agagtgccat ctaaatcaca taataagatt ttcatagttc
     4021 ctctttagga ataaagttga tttctccttg atatacttga acactggtta atattagttt
     4081 taaaggctgg cctaattgaa tctccttacc ttttttcccc actaactttg tcttgtccac
     4141 atcaagttca taatagtcct ttaagtcctc tagtcgtatc atgccttcaa tggtattatc
     4201 aagttccaca aaaataccaa agttggttac gcctgatacc ttaccttcaa attcttgccc
     4261 cactttatcc tgcatatatt ctgctttttt aatttcatcc atccttcgct cagcttcatc
     4321 ggcttttctt tctgcctcag agctatgctg ggccacctca ggcaaaatag ccttataatg
     4381 ctctctttgg cttttgctta taccaaggat tgcctctttt ataattcggt ggatttgtag
     4441 gtcaggatag cgtcttattg gagcggtaaa atgagaatag tgtgtaaggg ctagcccaaa
     4501 gtgaccaata agatcgtcgg tataacgggc ctgctttaaa tagcgaagaa tcttgcttac
     4561 aagcagggct tcttcaggtt ttccctttaa actttttaag agctcactaa gttgttttgg
     4621 atggatttcc tcaacgtccc cctttattaa atagccaaag ctatgcaaaa gattgtttag
     4681 ttctgccatc ttttccccat caggtttttc gtgggaccgg taaagaaagg ggacctccat
     4741 ccagctaaaa tgttcagaaa ctgttttatt tgtcagtatc atggcttctt caatcatctt
     4801 atctgccagg ccctgctctc ttctgtgtac ttctatcggc catccctcat catccattac
     4861 aaaatatggt tcattgcttc caaaatctat accgcctcta tcatctctat tttttattag
     4921 aattttgctt aagtcctcaa agcattccaa ctcttttctt actggctcca gttctttatt
     4981 ttcttttccc tctaaaaatt ctgttacatc atcataaata aggcgataag aagaagctag
     5041 taccgaagga aagatatccg atgataggac ctttcccttg ctatcaatat ccattacaac
     5101 agagagacag agcttgtcct cattaggatt aagagagcac agctcattag aaatctcttc
     5161 tggcagcatg ggtattaccc ggtctacaag gtagacactg ctggctcttt tataggcctc
     5221 acgatcgagg taggaacctt ccttcacata atggctaaca tcagcaatat gtacacccag
     5281 acggtatcct cccttatgcc ttttgatact gatggcatca tcaaaatcct tggcatcagg
     5341 accatctatg gtaaagacaa gatcttccac ccttttgcgc tggctaatct cctcctggga
     5401 gggactagaa aatcttttgg cctccctaat cacttccttg ggaaaatgca ctggtagatc
     5461 aaactcttcc acaacaagct taaagtctag tccaggacta tataattcgc ccagcttttg
     5521 aatgaccttt cctcttaatt ttgtattgcg acttccataa tcaataattt ctacttgaac
     5581 aatttcaccg tctattgctt tgtttctatt tttcttatca acaaaaacgc taaaggccag
     5641 tcggcgatta agggggataa caaaaccctt gctatggtag atccccatta ctgattggtg
     5701 ttttcttttg gttattttaa cgacttttcc ttctttgcga gccttgcgat tggaaggaat
     5761 aagctttata agaacttcat cgccatccat tgcattagaa aaatttgaag agtgtacaaa
     5821 gacatcctcc ttggacccct catcataaac aaaaaagcca taactacttc caggaatgga
     5881 catcttcccc gctaaaagac tcttgccctt atggggaaag taccttccct ttcttgtttt
     5941 tcttatttgt cctttatttt ctagcttttc tagctctaag agaagttcct ttacatcttc
     6001 tatctttagc gccctttgta tttcttccag actttgagat ctgttcttac tctttagtag
     6061 ttccaggatt tgcataagac ctccatcgct tatctttata aaaagattat ctcgtgattc
     6121 attttggcca ccttagctac cgtagcatgg atagagcaat attttccagc aaggctgcag
     6181 gccttagtaa atttcttttc ctctgtgact cccttagctt caatcacaaa atcaaccctc
     6241 tcaagagttg caatcttagc ctcttttttc tttccatcaa ttgttattgt tacttcctca
     6301 taatctattt tcatcttctc acaaagtgac tctaatgtgt agtagtaaca agcacttagg
     6361 gctccataaa gaagatcata gggcagatac tggccctcac cagagcctag agggattttt
     6421 cctctgccat tatctatcca tttttctgat ccatattttg atttaatcga catttattcc
     6481 tcctagtaaa ttgctttgtt taaaatttct tttaaacgat aatcatcatt tttaaaaaaa
     6541 ctaaaatacc gctggacatg gttttctcca ggctgaaaag aaacattttc cttaaaactt
     6601 tcatctaata taagggtgaa atctagtttc accataatta acagctcatc ttcatgctcg
     6661 attgtgatct catggacttc tccccctaaa acatgggaca aaaagccttc attatagctt
     6721 tttgcatcca aggaaaaagg cgctagaatt tcaagctgcc tttgtaaatc ctgagtgatt
     6781 agactctcat aataatcttc caccactcca agacacatct cttctggttt ttcctggctg
     6841 catgaactca taagaagtaa aaatataagc aataaaacac gcatagttcc tccacaaaag
     6901 tgaacaagtc taattctagg cagactagtt gctggactca gacaagaaaa gagtcaatcc
     6961 cttagcttta gccggcaacc aatattgctc cgccgacttg gcccttcttg ctagctggct
     7021 ttttagtctc tattggcttt taggctccac aaagcaattt catctctacc cacaaaagtc
     7081 tataccccat tcttgtcttc tttcaacaaa aaaggggctg ccccctagat tgttactact
     7141 ttttgcgcct tttgtatcat atcaagaatc tcttccatgc tagatattcg gcctactctc
     7201 agctgcttat ttaaatcaaa aaagttcacg cagctaccac aggccacaag atcaactcct
     7261 gcttcttcta aaaaatgaag agcctctaaa catcctcctg cgctagtaag aagtttaact
     7321 gccccattat aaaaaattat ggcttctggt tttattggac tggcccagag ttttttcaca
     7381 aatgcgataa ttaatctttt tcccaactcc tgtgagccac tgcccatggt atccttgttg
     7441 ataataatca cttctttcat agaccctcct ccttctagca taccatcact ttattgtatt
     7501 ttaaagcttt tttatgccaa ggcttgctag actctttcct ccgtaggaac tagctatgat
     7561 tggtccttgg caggctctag agaaaaagcc ctttagcttt ccattataaa caaaaagccc
     7621 tgtcatatga ttaaagcttt tcaccccttc ttcaaaactt aagatcttgt tagcaggtag
     7681 gtccgaaaag gcctgtatta ggtattcttt tcctacaagc ttttctaagg tggccttcca
     7741 ctcgccttct tgcatatcac tacctagata gattcctcta gaggcatagg aatcagtagg
     7801 tttaagaaga tatttatttt tttccttaac aaaatgagca tcagaagcta ccttttcatc
     7861 tattaaggcc gtaaagggga tatggttttc aataaatttt atttgctcta gggtaaaatg
     7921 ctcccttgcc cctggatggt gcaggacaaa aaagagcatc ttattatgga taatctggct
     7981 ggcaatgggg ccgacaagca ctgtcttagc tgattttatg ccttctacaa aatttggcaa
     8041 ctcatcatag attttaagaa aatccgaggt gacaaggcgc ctgtaaatat agtccagc
//
LOCUS       DASRNA010000211         4902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_73541,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000211 DASRNA010000000
VERSION     DASRNA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73541"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFD08_05955"
     CDS             complement(<1..757)
                     /locus_tag="VFD08_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HZK10597.1"
                     /translation="MKTKKEIIELRKHLMDNIRMEIDFSQTDQNLGIPMPAIQKEVSE
                     DDLVIALPPWEEVIKPQGSLDELIRKRRSYRKFSKKALIAEELSYLLWATQGIRRFHP
                     KRTLRSAPSAGNRHSLETYLALTKPLASRDGTVLFKRGLWRYLPIEHALVYLSSPKAL
                     EEKITEAALDQAFVAKAPIVFFWSTIPYRTEWRYNKASQKVIALDAGHVCQNLYLASE
                     SIACGTCAIAAYNQAEADALFDLSNPDEFILYMA"
     gene            940..2571
                     /locus_tag="VFD08_05960"
     CDS             940..2571
                     /locus_tag="VFD08_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZK10598.1"
                     /translation="MYKLIKNAKILTMDKQKPYAQAALVANEYFAYVGSEKGAREFLA
                     ERDLTAQEWDAKGHTVLPGFNDAHLHLLHCLKAKDSVDLFSCQSFKELMATMNEAYEN
                     HNKDSGSWLIGEGWNQDYFSDEKRFPLGKDLDKISRDIPIVIMRACFHIGVLNSKAME
                     LIGITKEKASDHYGYIDVDEKKNPTGVIREYVLDNIKAKLPSPSVEDLVEKLIDFQED
                     LFEQGITSVQSDDVKYLAGENYPRFCYLMREAGEKKRLKLRYSLQLLVDNKEDIEQFF
                     ALGFDKDFGNRRFKISSIKILTDGSLGARTAYLRKPYSDDPSTRGIALYKQNELNHMV
                     YSCHKENMPVALHAIGDGAIEQCLEAISYAQEQFPHLHPRHGLVHAQITDKDLLRRIK
                     KLKIGLYIQPIFLHYDMHMVKSRVGGALAQSSYAWKTCRDMGIPCAYSTDAPVEPFKT
                     MPNLYCAVTRRDLQARGPFLPEEKVSVEEALEAYTLLGAYMTAEEEDKGKIKEGYLAD
                     FIILDRSITEVEKKEILATRVMKTFLGGQVVYKRG"
     gene            2847..4523
                     /locus_tag="VFD08_05965"
     CDS             2847..4523
                     /locus_tag="VFD08_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZK10599.1"
                     /translation="MYNKDKTEELKAGLAQYNSQVKKAIERFPERKETFYTGSKEEVN
                     RLYTPLDLEGNDFDASIGFPGSYPYTRGVQANMYRGRLWTMRMYAGFATAEESNRRYK
                     FLVEQGSSGLSVAFDLPTQIGYDSDHSLSEGEVGKVGVAIDSLADMEILFDGIPLDKV
                     STSMTINAPAAILLAMYIAVAEKQGVSPEKLRGTIQNDILKEYIARGTYIFPTEPSMR
                     LITDIFAYCSEHVPQWNTISISGYHIREAGSTAAQEVGFTLADGIAYVEAALEAGLSI
                     DSFAPRLSFFFNAHNDLLEEVAKYRAARRLWAKIMKERFAAKNPKSMMMKFHTQTAGS
                     TLTAQQSENNIARVTIQTLAAILGGSQSLHTNSKDEALALPTEASVQTALRTQQIVAH
                     ESGVANTVDPLAGSYYIEAKTDEIEAAAREYIDRIDELGGAAKAIDDGYIQREIMEAA
                     YNYQKDIENEEAIVVGVNKYQVEENTPQNLLRVDPLVGENQKEKLGILRKERDQKAVD
                     LALAKLKKAAQGKENTMPLILEAVKTYATLGEITDVLREVFGEYKQTVII"
     gene            4533..>4902
                     /locus_tag="VFD08_05970"
     CDS             4533..>4902
                     /locus_tag="VFD08_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZK10600.1"
                     /translation="MQKPIRVLVGKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTA
                     EQIVHAAIQEDVDCVAVSILSGAHNTLLPKISKLLKEEGGEDILFVAGGVIPEVDIPF
                     LKEEGVKGIFTPGTPTKVSID"
BASE COUNT         1561 a          911 c         1106 g         1324 t
ORIGIN      
        1 gtgccatata gaggataaat tcatcggggt ttgaaagatc aaacaaagcg tccgcttctg
       61 cttgattata agcagcaatg gcacaggtgc cacaggctat ggactcagaa gctagataaa
      121 gattttggca gacatgtcct gcatctaggg caatgacttt ttgagaggct ttattatagc
      181 gccactctgt gcgatagggg atggtagacc aaaaaaatac gataggagcc ttggcaacaa
      241 aggcttgatc tagtgctgcc tcggtgattt tttcttctag ggctttagga gaggataaat
      301 aaaccagggc atgctctata ggaagatagc gccataggcc gcgcttaaaa agaacagtgc
      361 catccctact ggccaggggt ttagtaaggg caagataggt ttctaggctg tgacgattgc
      421 ctgctgaggg tgcactgcga agggttcttt ttggatggaa cctacgaatc ccctgggttg
      481 cccaaagtaa ataagaaagt tcttctgcta taagggcttt cttgctaaat ttccgatagc
      541 ttcttctttt tcggataagt tcatcgaggc tgccctgggg cttaatgact tcttcccagg
      601 gaggaagggc tatcacaaga tcatcttctg agacttcctt ttgtatggct ggcatgggaa
      661 tgcctagatt ttgatcggtt tgtgaaaaat ctatttccat acgtatgttg tccattagat
      721 gttttcttag ttcaattatt tctttttttg ttttcattct acttcctttc aaaaactttt
      781 gtcctaaaat acagactttc aagtataatt actataatag cagaagtaaa aaaataaaac
      841 agactaaagg gattaaaatc caaataactt tttgattact cttactaatt catgcttttt
      901 gtcagggcta gacagaccat ttagaaagaa agggctaaga tgtataaact aataaaaaac
      961 gcaaaaatac tgacaatgga taagcaaaaa ccctatgccc aggcagctct tgttgccaat
     1021 gaatattttg cttatgttgg aagtgaaaaa ggagcaagag agttcttagc agaaagagac
     1081 cttactgccc aggagtggga cgccaagggg catacagttc tacctggatt taatgatgcc
     1141 catctgcacc tacttcactg ccttaaagcc aaagactccg ttgatctttt ttcttgccaa
     1201 tcatttaaag agctgatggc cacaatgaat gaggcctatg aaaaccataa taaggattct
     1261 ggatcttggc ttattgggga aggctggaat caagattact ttagcgatga aaaacgtttt
     1321 cccttaggga aggacctaga caagatctca agggatattc ccatcgtgat tatgcgggcc
     1381 tgctttcata taggagttct taatagcaag gctatggagc tgattggaat aacaaaggaa
     1441 aaggcctcag atcattatgg atatattgat gtggatgaga agaaaaatcc cactggagtg
     1501 ataagggaat atgttcttga taatattaag gccaagctac cttctcctag tgtagaagac
     1561 ttggttgaaa aacttataga cttccaagaa gatttatttg agcaaggcat tacttctgta
     1621 caaagcgatg atgtaaaata cctggctggg gaaaattatc ctaggttttg ctatttgatg
     1681 cgagaagctg gggaaaagaa aagattaaaa cttcgctata gcttgcaact tttagtggat
     1741 aataaagaag atatcgagca gttttttgcc ctaggctttg ataaagactt tggaaataga
     1801 agatttaaga tatcctctat aaaaattcta actgatggct ccctgggggc tcgtactgcc
     1861 tacttaagaa agccctatag tgatgatcct tcgacaaggg ggattgccct ttataagcaa
     1921 aatgaattaa accatatggt atatagttgc cataaggaaa atatgccagt ggcccttcat
     1981 gccataggag atggagcaat tgagcagtgt cttgaggcta tatcctatgc ccaagaacag
     2041 ttccctcatc tccatcctcg ccacggtctt gttcacgctc aaattaccga taaagacctc
     2101 ttaagacgca taaaaaagct aaagattggc ctctatattc aaccaatctt tcttcactat
     2161 gatatgcata tggttaaaag tcgggttggt ggggccttgg cccaaagctc ctatgcatgg
     2221 aagacttgtc gagatatggg aattccttgt gcctatagca cagatgcccc agtagagccc
     2281 tttaagacca tgcccaacct atactgtgct gtaacaagaa gggacttaca agctaggggc
     2341 ccctttcttc cagaggaaaa ggtttcagtg gaagaggctc tagaagccta cactttacta
     2401 ggggcctata tgacagcaga agaagaagat aaaggaaaga tcaaagaagg ctatttggca
     2461 gattttatta tactagaccg ttctattact gaagttgaaa agaaagagat tcttgctaca
     2521 agggtaatga aaacctttct tggtggtcaa gtggtttata agagaggata gattcttatt
     2581 catttgtgta aaaatcttta gagaaagtag cctctatctc tttaaaatgc tagcttagcc
     2641 tttatggtat tataagatag aagaaaaaaa ggcaagagga atatgcaaca aatgttgcat
     2701 aaggtttaaa aaacgctaaa agtgcaagaa atcatatcta ttgcatctag tctatgctat
     2761 actacagtcg atatcttgta tcagttcagc atttaaataa aggcttttga tacaaacact
     2821 ttcgcgtttt ttgggaggaa tgtgatatgt ataataagga taaaacagaa gaattaaagg
     2881 caggcttggc ccaatataat agtcaagtta agaaggccat agaacgattt ccagaaagaa
     2941 aagagacctt ctacactgga agcaaagaag aagtaaaccg cctctataca ccactagatc
     3001 ttgagggcaa tgactttgat gcgtccattg gttttcccgg cagctatccc tatacaaggg
     3061 gcgtacaagc caatatgtac agaggacgcc tgtggactat gcgtatgtat gctggttttg
     3121 caacagccga ggaatccaat cggcgctata agtttttagt agaacaaggt tcatcaggtt
     3181 tgagtgtggc ctttgatttg cccacacaga ttggatatga ttctgatcac agccttagcg
     3241 agggggaagt aggtaaggta ggtgttgcta ttgactccct agcggatatg gaaatcctct
     3301 ttgatgggat tcccttggac aaggtttcta cttctatgac tataaatgct ccagcggcca
     3361 tactcctagc catgtatatt gctgtagcag aaaaacaagg agtcagtcca gaaaagcttc
     3421 gagggacaat tcaaaatgat atcttgaagg agtatattgc caggggaacc tatattttcc
     3481 caacagaacc ttccatgcgt cttattacag atatttttgc ctattgctct gaacatgttc
     3541 cccagtggaa taccattagc atttcgggct atcatataag agaagcagga agcacagcgg
     3601 cccaagaagt tggatttacc ctagcagatg gcattgccta tgtggaggca gccctagagg
     3661 ctggtctttc tatagattcc tttgctccaa gactttcttt tttctttaat gctcacaatg
     3721 accttttaga agaagtggcc aaatatcgcg ctgcaagaag actttgggca aaaattatga
     3781 aggaacgttt tgctgctaaa aacccaaaga gcatgatgat gaaattccac acccaaacag
     3841 cgggaagtac cctaacagcc caacaaagtg aaaataatat agctcgtgtt acaatacaaa
     3901 ctcttgctgc cattcttgga ggaagccaat cactacacac caatagtaag gatgaggccc
     3961 ttgccctgcc aacagaagcg tctgtgcaaa cagccctgcg cactcaacag attgtagccc
     4021 atgaatcagg ggttgctaat actgtggatc ccttggctgg aagttattat attgaagcaa
     4081 agacagatga aattgaagca gcagcaaggg agtatataga ccgaattgat gagcttggtg
     4141 gagcggctaa ggcaattgac gatggctata tccaaagaga aataatggaa gcggcctata
     4201 attatcaaaa agacattgaa aatgaagagg caattgtggt gggggtaaat aagtaccaag
     4261 tagaagaaaa caccccccaa aatcttctta gggttgatcc tcttgtggga gaaaatcaaa
     4321 aagaaaaact aggcatcctc cgcaaagagc gagaccaaaa agcagtagat cttgctctag
     4381 ccaagctaaa aaaggcagct caggggaaag aaaacaccat gcccttaatt ttggaagcgg
     4441 ttaaaaccta cgccacccta ggtgaaatta cagatgtctt aagagaagta tttggcgaat
     4501 ataagcaaac tgtgattata taaggagaaa aaatgcaaaa acctattcgt gttttagtgg
     4561 gaaaaccagg actagatggt catgacaggg gggctaaggt aattgcccgc gccttgcgtg
     4621 atgctggaat ggaagtaatt tatacaggcc tgcgccaaac ggcagaacaa attgtacatg
     4681 cagccattca agaagatgta gactgtgttg ccgtaagtat tttaagtggc gctcataata
     4741 cactactacc aaaaatcagt aaacttttaa aagaagaggg tggagaagat attctttttg
     4801 ttgccggtgg ggttattcca gaagttgata ttcccttttt aaaagaagaa ggagtaaagg
     4861 gaatttttac accgggaacc cctacaaaag tcagtattga tt
//
LOCUS       DASRNA010000212         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_740758,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000212 DASRNA010000000
VERSION     DASRNA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740758"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFD08_05975"
     CDS             complement(<1..262)
                     /locus_tag="VFD08_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10601.1"
                     /translation="MKNFIKTLIIPLLILFIRPMGLTVNQSIVLAALFLTVTWWTFGT
                     IKKSYASLFLLAMFTLFGQTPGLLVFDFLLSETMLIIIFSYIF"
     gene            749..1702
                     /gene="dusB"
                     /locus_tag="VFD08_05980"
     CDS             749..1702
                     /gene="dusB"
                     /locus_tag="VFD08_05980"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZK10602.1"
                     /translation="MIGFFPSKLLLGPMAGVTDYAFRRLLREMGLEYSVSEMISAKGL
                     CYGNDKTILYARAHKTDHPLALQLFGSEVEFMLKAALYLEKHFEYEVLDINMGCPVKK
                     IVKNGDGSALMKDTKLAMEIVYKLAENTSKPVSVKIRTGINEERKNCVEFAKAMEKAG
                     ASMVVVHGRTASQMYSGRANWNDIGRVKQALEVPVIGNGDIDSLEEGLNRIKDYDVDG
                     VMIARALRGNPFLVRDYKNYFTEGKLPEEVSIKERFSTMQTHLQYLIEERGETNGTAA
                     FRKHGLWYIRDFPGSAKIRDKLAHLKKAEDLQEIISTVLDR"
     gene            1747..2235
                     /gene="greA"
                     /locus_tag="VFD08_05985"
     CDS             1747..2235
                     /gene="greA"
                     /locus_tag="VFD08_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZK10603.1"
                     /translation="MKETILTQSGLEKIEKELEELKVYKRKEIAERIKEARSFGDISE
                     NAEYDEAKNAQAELELEIEKLENLIKFSRIIDEKDIPEDIVFIGHKIKLRNMETKKDE
                     EYLIVGNRESNPFESKISHESPLGKALIGKKKGDVVTVHLPAGLVRYKIKNMGKHNQN
                     QL"
     gene            2256..3731
                     /gene="lysS"
                     /locus_tag="VFD08_05990"
     CDS             2256..3731
                     /gene="lysS"
                     /locus_tag="VFD08_05990"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZK10604.1"
                     /translation="MTQETDLRQIRIDKLNKLKEKGKNPFLQESYDQDSFAKDILANY
                     TEYEGKTVAIAGRLMQKRLMGKAAFINIQDSTDRIQIYVRKDDIGEELFDEFKAYDIG
                     DILGIKGSVFRTKKEEISIKAEEITLLTKSLFTLPEKHHGLKDPELRYRQRYVDLIMN
                     PEVKETFIKRNRIIRGIRTFLDERNYLEVETPILDTVAGGANARPFKTYHNTLGMDMQ
                     LRIANELYLKRLIVGGFDRVYEMGKMFRNEGISTRHNPEFTNIELYAAYEDNAYVMKL
                     TEDLVEYLALELYGTTELNYQGRTIDVKTPWRRVSMHKFVEEKTGVDFYSFSDLEKAK
                     DVAKNKLHLELEPYAKIGHIIEAAFEEYCEADLIQPTMVMHHPTEISPLSKRNPDDPR
                     YTNRFEAFANGWEIANGFSELNDPLDQRKRFEDQMKQGEKGDEESHPMDEDFIQALEV
                     GLPPTGGLGIGVDRLIMILTNSPSIRDVIFFPTMKPLSTDE"
     gene            4039..>4564
                     /locus_tag="VFD08_05995"
     CDS             4039..>4564
                     /locus_tag="VFD08_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HZK10605.1"
                     /translation="MLRVEKREGYKVEFDKTKISSAIQKAMAETEKGIDATLADEISE
                     NIEDMVRNSGRIISVDEIQNLVEEKLMGSVRKDVAKRYIIYRNEKDKTRPLRKRKGEG
                     GEVLSEVFLRKYKHAPSPMNQLGNFVYYRTYSRWIPHEKRRENWWETVKRAVEYNCTL
                     VPTTTSEAEELFDNI"
BASE COUNT         1617 a          729 c          980 g         1238 t
ORIGIN      
        1 aaaatatata agaaaaaatg attataagca ttgtttcaga taagagaaaa tcaaaaacca
       61 ataagcctgg tgtttgtcca aaaagagtga acattgccaa taaaaaaagt gatgcatagg
      121 attttttgat tgttccaaag gtccaccaag ttactgttaa gaataaggca gctaatacaa
      181 tactttgatt tactgttaag cccattggcc ttataaataa aataagaagt ggaatgatta
      241 atgttttaat aaagttcttc acatattcct cctactaagt tcttgcctag tctgtaaaga
      301 taagtgatgg tgattgtact ctatgtgaat aaggctaaaa cgtattttta gctcagtgca
      361 gcagggaagc tcctataggt gtgtaaaaga aaaagaccag gggtgactta cctaagccaa
      421 atacatttta tgttaaaaca agaaaaaact caaagtataa tcggaaaatt agataaaaag
      481 agtccttaaa agcactttgg ctatactttt tatacttagc ttgggaacta gaagtcaaag
      541 atggaagaaa aagatagtag atgaaagcaa gtagggaaaa ggtgaaagtg aaactaatta
      601 aaaagtgctt aggtggagca ttatgtaagg ggtgatttaa ttagaggaga ccaggaggct
      661 tgttccctat cttttttgtt cattatatgc taaagtggta aggaaataaa taggaaaata
      721 ggcctattaa atatatttgg gaggtagtat gatagggttt ttcccctcaa aactgctctt
      781 aggtcccatg gcgggagtta cggactatgc ctttaggcgc ctacttcgag aaatgggtct
      841 tgagtacagt gttagcgaga tgattagtgc caagggtcta tgctatggta atgataaaac
      901 catcctttat gctagagccc ataaaaccga ccacccacta gcccttcagc tttttggaag
      961 tgaagtagaa tttatgctaa aggcagctct ttatttagaa aaacattttg agtatgaagt
     1021 gctagatatc aatatgggtt gtccggtaaa aaaaatcgtg aaaaatggcg atggctcagc
     1081 cctaatgaag gacaccaagc tggctatgga gattgtttat aaattagcgg aaaacactag
     1141 caaacccgtt agtgtgaaaa ttagaactgg cattaatgaa gaaagaaaaa actgtgtaga
     1201 atttgccaag gcaatggaaa aagccggtgc ctctatggtg gttgttcatg gtagaactgc
     1261 aagtcaaatg tatagcggta gagcaaactg gaatgatatt ggcagagtca aacaagccct
     1321 tgaggttcca gtaataggaa atggtgatat tgactcatta gaggaaggtc ttaataggat
     1381 aaaggactat gatgttgatg gggttatgat tgcaagagca ctgcgaggaa atcccttttt
     1441 agtgcgggac tataaaaatt actttactga gggcaaatta ccagaagaag tttctatcaa
     1501 ggaacgtttt tcaactatgc agacccatct ccagtatctt atcgaagaga ggggagagac
     1561 aaatggcaca gccgctttta gaaagcacgg actttggtat attagagatt ttccaggcag
     1621 tgcaaaaatc cgtgataaac tggcccattt aaaaaaggct gaagacttgc aagagataat
     1681 atcaactgtt cttgacagat aagaattgat gacataaaat ttaatatatt ttagtggagg
     1741 tagagagtga aggaaactat tttaacccaa tcaggtttag agaaaatcga aaaagagcta
     1801 gaagagctaa aggtttacaa gcgcaaagaa atagcagaaa gaatcaaaga agcaagaagt
     1861 tttggtgata tctcagaaaa tgcagaatat gatgaagcta aaaatgcaca agctgaacta
     1921 gaacttgaaa tagaaaagct tgagaattta attaaatttt cgaggattat cgatgaaaaa
     1981 gatattccag aagatatcgt ttttattggt cataagataa aactccgcaa tatggaaacc
     2041 aaaaaagatg aagaatacct tatagtggga aatcgggaat caaatccctt tgaatcaaaa
     2101 atttcccatg aatccccctt gggtaaagcg cttataggta aaaagaaggg tgatgtagta
     2161 actgtgcatc ttccggcagg acttgttcgt tataaaatta aaaacatggg caaacacaac
     2221 cagaatcaac tataaattgt aaaggaagga aggccatgac ccaagaaaca gatcttcgac
     2281 aaatacgcat tgataaattg aacaaactaa aggaaaaggg aaaaaacccc tttcttcaag
     2341 aaagctatga ccaagatagc tttgctaaag atatactagc taactataca gaatatgagg
     2401 gtaaaaccgt tgccattgca ggcaggctaa tgcaaaaacg gctaatgggc aaggctgcct
     2461 ttataaatat tcaagactcc accgatcgca tacaaattta tgtaagaaaa gatgacatag
     2521 gcgaggaact atttgatgaa ttcaaggcct atgatattgg agatatatta ggaattaaag
     2581 gaagcgtctt tcggacaaaa aaagaggaga tttctatcaa ggcagaagaa atcactcttt
     2641 taaccaagtc cctttttact ttgcccgaaa agcaccatgg cctcaaagat ccagaactgc
     2701 gatatcgtca gcgttatgta gaccttatta tgaatccgga agtcaaagaa acctttatta
     2761 aacggaatcg aatcatccga gggataagaa cttttcttga tgagagaaat taccttgagg
     2821 tggagacccc gattctggat actgtagcag gtggagcaaa tgcccgccct tttaaaacct
     2881 accataatac cctgggtatg gatatgcagt taaggatagc caatgaactt tatttaaaaa
     2941 ggctcattgt tggtggcttt gatagagtat atgaaatggg aaagatgttt cgtaatgaag
     3001 gaatttcaac cagacacaat ccagaattta caaatataga gctctatgct gcctatgagg
     3061 ataatgccta tgttatgaaa ctcactgagg atcttgtaga gtatctagct cttgaacttt
     3121 atggaacaac ggagctcaac tatcaaggta gaacaattga tgttaaaaca ccttggcgac
     3181 gagtgtctat gcataaattc gtagaagaaa aaacaggcgt tgatttttat agctttagcg
     3241 acttagaaaa agccaaggat gtggcaaaaa acaagcttca tttggaattg gaaccctatg
     3301 ctaagatagg tcatattatt gaagcagcct ttgaagagta ctgcgaggca gatcttattc
     3361 agccaacgat ggtaatgcac catcctactg aaatctctcc gctttctaag agaaaccctg
     3421 atgatcctcg ttatacaaat cgctttgaag cctttgccaa tggctgggaa atcgcaaacg
     3481 gttttagtga gcttaatgac ccccttgatc agcgtaagcg ctttgaagat cagatgaagc
     3541 agggtgaaaa gggtgatgaa gaaagccatc ctatggatga agactttatt caggccctag
     3601 aagtgggact acctcctaca ggaggattgg gtattggggt agatcgtttg attatgattc
     3661 ttacaaactc tccttctatt agagatgtta tctttttccc aacaatgaag cccctatcaa
     3721 cagacgaata ataataactc tatgaggaaa gccttagttg gactttcctc atttttattc
     3781 ttttaacgct cttttaggga ttttcttctt atataaactt tctttttaat tagaagatac
     3841 ttcaacttaa aaggtgctgg agtgattggc cagtattttt tttatcttct aaagcgctta
     3901 tttcatggat tttcatagat tggagtcgct ttctaccaat aaaacaaaat aaaagacttg
     3961 ccttcttagc ccctgggata taatatatat agtgtactca tattaattag atactatatc
     4021 ttgtgtttgg agggatttat gttgcgagtt gaaaaacgtg aaggctataa agtagagttt
     4081 gataaaacaa agatcagctc tgctattcaa aaagccatgg cagagacaga aaaaggaatc
     4141 gatgccactc ttgctgatga aattagcgag aatatcgagg atatggtgag aaattctgga
     4201 cggattatat ccgtggatga aattcaaaac ctagtggaag aaaaactaat gggaagcgta
     4261 agaaaagatg tggcaaaacg ctatattatt taccgtaatg aaaaagacaa aacccgccct
     4321 ttgcgtaagc gaaaaggaga aggtggtgag gttctaagtg aggttttcct tagaaaatac
     4381 aaacatgccc cttcccccat gaaccaattg ggtaactttg tttattacag aacatattca
     4441 cggtggattc cccatgaaaa aagaagggaa aactggtggg aaactgtcaa aagggcagta
     4501 gagtacaact gtacattggt acccacaaca accagtgaag ccgaagaact ttttgataat
     4561 atat
//
LOCUS       DASRNA010000213         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_665867,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000213 DASRNA010000000
VERSION     DASRNA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665867"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VFD08_06000"
     CDS             <1..866
                     /locus_tag="VFD08_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZK10606.1"
                     /translation="SMHACGHDGHIAIGLNFAKWLVNNQDSLKGKFVLIFQPAEEGVR
                     GAKSMVEAGVVDGVDYLLAGHIGSGSGKNVLGVGSQGFFATSKMDIYFEGVPSHAAGS
                     PEHGRNALLAAASCALNLYTLTQFSAGMSRLNVGVLRAGSSRNIIANKAMMQIETRGE
                     NQIVNDMLREKVNYAIEGAAKSFGVDYRIKLAGGAPSYTVKDPAFIKSVATALSEKGF
                     ATDTNIAIGGSEDVTYMMNEVENGGGKAIHFIFGTHIKAQAHHHKFDFDEEVLDFATR
                     SLAETVKYLIG"
     gene            complement(941..2128)
                     /locus_tag="VFD08_06005"
     CDS             complement(941..2128)
                     /locus_tag="VFD08_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZK10607.1"
                     /translation="MKKKIDGLIDKHKDEIIAIRRDIHQHPELGMEEVRTSGLVKKEL
                     RGLGLEVHDGIGKMGVVGILRGKKEGRTLLLRADMDALPIQEQTDLSFASKEAGKMHA
                     CGHDVHTAILLGVAKVLSELKDQIQGNIKFVFQPAEESNPTGGARYMIEDGVLDNPKV
                     DGAMALHIKNIPLGQVALRPGVMSAKSDRIYLTVKGKSAHASEPHKGNDAIVAAAQIV
                     SNLQSIVSRNVPPMESAVITLGTIHGGSRYNVICDQVLMEGTVRTFHPQVSKLMPKRI
                     KEVASKIAQALDCSCEIDYVEGYTNTINDPKMTKEVIDLFKENLGEENVLIPEEPSSG
                     GEDFSEFSKRVPSVYYWLGMESEINKRKALLHNPHLIVDERSIPIGMKTLCLAAMDFL
                     EDA"
BASE COUNT          691 a          539 c          482 g          733 t
ORIGIN      
        1 gatcaatgca tgcctgcggt cacgatgggc atatagccat tgggcttaac tttgccaagt
       61 ggcttgtaaa taatcaggac tcactaaagg gaaagtttgt tttaatcttc caaccagcag
      121 aagagggggt tcgtggggcc aaaagcatgg tagaagctgg cgtggtagat ggtgttgact
      181 atcttcttgc aggccatatt ggaagtggat caggaaaaaa tgttctaggg gttggttccc
      241 aaggcttctt tgctactagc aaaatggata tctactttga aggtgtgcct tcccacgctg
      301 caggaagccc agaacatgga agaaatgccc tattagcagc agcaagctgt gcactcaatc
      361 tatataccct cacccaattt agtgctggca tgagtagact caatgtgggc gttctaagag
      421 cgggatcttc acgcaatatt attgccaata aggccatgat gcaaattgaa acccgcggag
      481 aaaaccaaat agtaaatgat atgctcaggg aaaaagtaaa ttacgccatc gagggagcag
      541 caaaatcctt tggtgttgac taccgtataa agctcgctgg aggagcaccc tcctatacag
      601 taaaagatcc agcttttatt aaaagtgttg ccacagccct ctcagaaaaa ggatttgcta
      661 ccgatacaaa tattgccatt ggtggttctg aggatgtaac ctatatgatg aacgaggtag
      721 aaaatggcgg aggaaaagcc attcacttta tttttggaac ccatataaaa gcacaggccc
      781 accaccataa attcgacttt gatgaggagg tcctggactt tgctactaga tcactagcag
      841 aaacggttaa ataccttatc ggctagtaaa aaagaccctc taataaaaag atccccctaa
      901 gttggactag aatccaagct agggggtctt ttaactttat ttaggcatcc tctaaaaaat
      961 ccatcgccgc cagacaaagg gttttcattc caattggaat ggacctttca tccacaataa
     1021 gatggggatt atgcagtagg gctttccttt tatttatttc tgattccata ccaagccagt
     1081 aataaacaga gggaaccctc ttggaaaact cggaaaaatc ttctcctcca gaagaaggct
     1141 cctcgggaat aagtacattt tcctctccta aattttcttt aaataagtca atcacttctt
     1201 ttgtcatctt aggatcattt attgtatttg tatagccctc aacatagtca atctcacagc
     1261 tacaatccag ggcctgggca atcttgcttg caacttcttt gattcttttg ggcataagtt
     1321 ttgatacttg cgggtggaag gttcgcactg taccctccat aagcacctga tcacaaatta
     1381 cattataacg acttcctcca tgaatggtac ctagggtaat aacagcactt tccatgggtg
     1441 gaacattgcg acttacaatg ctttgaaggt tgctgacaat ctgagctgct gcgacaatag
     1501 catcatttcc cttatgtggt tctgatgcgt gggctgattt tcccttaaca gtaagataaa
     1561 ttcggtcgga cttagcactc attaccccag gacgcagggc tacttggccc agtgggatat
     1621 ttttaatatg cagtgccatg gctccatcta ccttgggatt gtctaagacc ccatcttcta
     1681 tcatataacg agctccacca gtgggatttg actcttcagc tggctggaac acaaatttta
     1741 tattcccctg gatttgatct ttaagttcac ttagaacctt ggctacgcca agaaggatgg
     1801 ctgtgtgaac atcatgtcca caggcatgca tcttccccgc ctctttcgat gcaaaactta
     1861 aatctgtttg ctcttgaata ggaagagcat ccatatctgc acgaagcagc agggtccttc
     1921 cttctttttt tcctcttaag attcctacaa cacccatttt tcctatccca tcatggactt
     1981 caagacccag ccctcttaac tcttttttta caagcccaga agttcttact tcttccatgc
     2041 ccagttcggg atgctggtgg atgtctctac gtatggctat gatttcatct ttatgcttat
     2101 cgattaaccc atcaattttt ttcttcattt attctcctcc tgagtttaag gccaaatgac
     2161 tagttaaaaa ttattactag aagatttggc taataggtag cttattacga tgatactata
     2221 agagctccta agtcacaatc ttatcgtaaa agctccttga tatgttctta atagtaacta
     2281 gaccacccct cacatcgttt tctctcccct attcagcaga aaataagtgg acctcttagg
     2341 ttttttaaag aaaatttata aaaacaaagt ggaaatagcc tctgtcttta ttacaatata
     2401 catatcttta tcgtttagcg aatggcgttc ttttttcatt tattg
//
LOCUS       DASRNA010000214         6519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_215450,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000214 DASRNA010000000
VERSION     DASRNA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215450"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFD08_06010"
     CDS             <1..383
                     /locus_tag="VFD08_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10608.1"
                     /translation="RGPCPDYIIVGLGLNLYRPEEGFPQELNLAGALLEKKQEGLLNL
                     LVKQILVEFWPFYDQLDQRHFLEEYRSRNLVLGKEVTILSKKPWTGRAIEIDDSCHLI
                     VEDSKGQHHKLNSGELSIKVGADD"
     gene            complement(390..1730)
                     /locus_tag="VFD08_06015"
     CDS             complement(390..1730)
                     /locus_tag="VFD08_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK10609.1"
                     /translation="MKKEMKLNYQNTSLIGFGFFASSLVWAIYNSFVPLLLQERFLLT
                     TTSIGLIMTIDNFFGVIFQPLVGSVSDKTVTARGRRMPWIMVGLPLSAIAFSFIPKMY
                     TLVSMMLVIITFNILMSLWRAPVIALMPDVTPKPLRSKANGVINLMGGIGSITAFLLG
                     GLLAKRSPDLSLPFLFATFVMFVSLLTLIAFVREPVGLVFRNARGDKLNPRERKLLDQ
                     AQSHGLGTIDLSAKESDSSKKDKHFFAPLKKLEGAEKKSLLFLLGAIFFWFSGFNAVE
                     TFFTLYATNTLGMSSGSAAMTLASFSLAFLVFALPSGILAEKFGRKPVILTGLVGIIV
                     MFIPMLFVQNTTLITVLLIGGGLFWACININSLPMVVEMTTEESLGSFTGYYYFFSFS
                     ASIASPILFGWIRDLTQDYTTLFAYSIVAFILAFVLMIFVKHGDNLTLAYKKSR"
     gene            complement(1834..2868)
                     /locus_tag="VFD08_06020"
     CDS             complement(1834..2868)
                     /locus_tag="VFD08_06020"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10610.1"
                     /translation="MANINIRELAEVNGGINVSIYLPTHRKAPENKQDRIRFKNLINQ
                     AEKELEEKFEMKNPSTFLKEAENLYQDLMFWAHATDGLALLINEEETLVFKLVGTIPE
                     RLVVGERFHLLPLLNYYEFINESYILDISKDRFKLYFGSHEGVVEVETPGIEQSFDEL
                     FDDQDIQSTNYYSERASKPGLDRIETEKYFRYVIKKLANFLEDDAPLLVFGTTEVLSV
                     FSDYSKDRLDILRLIDKPLGSLQTNQIYGKLKDILLPQYKEEVENHLLNLNDEVARDR
                     GTDNESLVIHQAPSGRIETLFIAEDMGGLIEEEADKLVQNVLTTDGEVVLIDESITPF
                     PMGVGAIFRY"
     gene            3401..4576
                     /locus_tag="VFD08_06025"
     CDS             3401..4576
                     /locus_tag="VFD08_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HZK10611.1"
                     /translation="MKNSEILKEMIKKLHKGASVDDLKGEFRKHFKDVPAQEIADAEK
                     ELMEEGYTVEEIQSLCDVHASLFENRVQIEVKSKELGHPLHIFKEENKGLLDFLANKF
                     DPLFEDYKKDPQGKKDSMLYGLKELSKVDTHYSRKENLLFPFLEKAGVTAPPKVMWGV
                     DDEIRAFLKEALAAVKEENVENATKSVISAREQIKSMITKENDILMPLLSKHVDDEGW
                     KLVAQESLHFGYVFTGDRENASPSDAAAWLKKGSTVTQPRNDAPIQLASGFFTVNELT
                     TLFNTLPCDITFVGADDKVHYFSESKDRVFPRTRTIIGRDVEDCHPPKSLDRVSDIVD
                     DFKAGKKDTESFWIKMGEKFILIRYYALRDEEGSYLGVLEVSEDIAPLRALEGEKRL"
     gene            4991..6004
                     /gene="sppA"
                     /locus_tag="VFD08_06030"
     CDS             4991..6004
                     /gene="sppA"
                     /locus_tag="VFD08_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZK10612.1"
                     /translation="MNKKRWIAVALSVLVFISSFVVSSKGKSKIIEKETTSLLKELMS
                     EELSKTVLRPGNANQRIAVLHIRGTILNMEGNWLNASQYNHNLTINSLDSIRNDETVK
                     GIIIRIDSPGGGVYESAQLSDKIGEIKRTRNIPVYVVMESMAASGGYYIAAGADQIYA
                     TEETLTGSIGVIMSGMNYSGLMDKLGVEDMTIKSGEMKDVGSLTRETTKKDLEVLQEL
                     VDNMYERFVDVVVEGRDMDRAKVYKLADGRIYDGAQAKNNGLVDKLGYYDEALADFEK
                     AYGLQEAEVFSYDNMGLDKISQLFMKSANLFNPRKEINVGNIELPASWKESSGFMYIY
                     GGY"
     gene            6006..6485
                     /locus_tag="VFD08_06035"
     CDS             6006..6485
                     /locus_tag="VFD08_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZK10613.1"
                     /translation="MEKYHNYPSIVYAGFWIRFFAYCTDLIMIGSLNRMLLFFLEEGR
                     VKTIASLGVFLLYFLLMTKLNNGQTIGKMIFGLRLVSFSQEKLSWTTVVVREIFGRYL
                     LKSYAIIYLITAFSEKKQHGIDMLLDTTVISENSLQLYKEIPINIGEEVKREELAAY"
BASE COUNT         2231 a         1143 c         1316 g         1829 t
ORIGIN      
        1 caagaggtcc ttgccctgat tatataatag tgggccttgg cctcaatctt tataggccag
       61 aggagggctt tccacaggaa ctaaatttag caggagccct attagaaaaa aaacaagaag
      121 gcttgttaaa ccttcttgta aagcaaatac tggtagaatt ctggcctttt tatgatcaat
      181 tagaccaaag gcacttcctc gaggagtatc gcagtcgaaa ccttgtacta ggcaaagaag
      241 taaccatact ttccaaaaag ccttggactg gtcgggccat agaaattgat gactcctgcc
      301 atcttattgt agaagatagt aagggccaac accataaact taattccggt gaacttagca
      361 ttaaagtagg agcagatgat tgatctagat tatctgcttt ttttgtaggc caaagtaagg
      421 ttgtctccat gttttacaaa aatcataaga acaaaggcca aaataaaagc cacaatagag
      481 taagcaaaaa gtgtggtata gtcctgggtt aaatctctaa tccaaccaaa taaaataggg
      541 gatgcaatag aggcactaaa ggaaaagaaa tagtagtagc ctgtaaagga ccctaggctt
      601 tcttcggtag tcatttcaac aaccatgggt agggaattga tattaataca agcccaaaaa
      661 agaccaccac caattaaaag aacagtaatt aaagtagtgt tttgcacaaa gagcatgggg
      721 ataaacatta caataatacc aaccaaacct gtcaaaatta caggctttct accaaacttc
      781 tcagcaagaa ttcctgaagg aagggcaaag accaaaaaag caagggagaa gctggccaag
      841 gtcatagccg cacttccact actcattccc agggtattgg tagcataaag ggtaaaaaaa
      901 gtttctactg cattaaagcc actaaaccag aagaagatag ccccaagtaa aaacaaaagg
      961 ctttttttct ctgccccttc taactttttt aagggagcaa aaaaatgctt gtcttttttg
     1021 cttgagtcac tttctttagc tgaaagatca atggttccaa gtccgtgact ttgtgcctgg
     1081 tctagaagtt ttctttctct gggatttaat ttatcccctc gagcatttcg aaagacaagt
     1141 cccacaggct ctcttacaaa agcaataaga gtaagcaggg aaacaaacat aacaaaggtg
     1201 gcaaaaagaa agggtagaga taaatccgga cttctttttg caagaagacc ccccagcaaa
     1261 aaggctgtga tagatccaat gcctcccatt aggttgatta caccattggc cttactacgc
     1321 aaaggctttg gggtaacatc tggcatcagg gctataaccg gagcccgcca caaactcatt
     1381 aggatattaa atgtgataat aacaagcatc atagaaacaa gagtatacat tttaggaata
     1441 aaggaaaaag caatggcaga taaaggtagg cccaccataa tccatggcat gcgccttcct
     1501 ctagcagtga cagttttatc gcttacagaa cctactaggg gctggaaaat tacaccaaaa
     1561 aaattatcaa tggtcataat cagtccgata ctggttgtag taagtaaaaa ccgctcttga
     1621 aggagcaaag gaacaaagga attgtaaatt gcccagacaa gagaagaggc aaaaaaccca
     1681 aagccaatta ggcttgtgtt ttgatagttt agtttcattt cttttttcat agaaactcct
     1741 ttctatggga tagtataaca ttttattttt ataaatacag taattttggc aataaaaaac
     1801 cgggactgat tattagtccc ggtagcaaag aaattaatat ctaaaaatgg ctcccacacc
     1861 catgggaaaa ggcgtgatgg attcatcaat aagtactacc tctccatcag tagttagcac
     1921 attttgcact agcttatctg cttcctcttc aatcaagccc cccatatctt ctgctataaa
     1981 gagggtctcg attctaccac taggggcttg gtggataaca agggactcat tatcagttcc
     2041 cctatctcga gccacttcat catttaaatt caagaggtga ttttccactt cttctttata
     2101 ctggggaagc aaaatatctt taagcttgcc ataaatttgg ttggtttgta aagacccaag
     2161 gggtttgtca attagtcgaa gtatatctaa tctatcctta ctgtagtcac taaagactga
     2221 gagaacttca gttgtaccaa aaacaagcag gggagcatca tcttctagga aatttgcgag
     2281 ctttttaatt acatatcgaa aatatttttc tgtttctatt ctatcaagtc cgggcttaga
     2341 ggccctttct gagtagtaat tggtgctttg aatgtcttgg tcatcaaaga gttcatcaaa
     2401 actttgctca attcctgggg tttcaacttc cactacacct tcatgactgc cgaagtagag
     2461 cttgaatcta tcctttgata tatctaatat ataagattca ttgataaact cataatagtt
     2521 taataggggc aagagatgga aacgttcccc tacaactaaa cgttcgggaa tagtacctac
     2581 gagcttaaaa accaatgttt cttcttcatt aattaatagg gctaagccat cggtggcatg
     2641 ggcccaaaac ataaggtctt ggtagagatt ttctgcctcc ttaaggaaag tcgaagggtt
     2701 tttcatctca aacttctctt ccaattcttt ttctgcctga ttgatcagat ttttaaaacg
     2761 gatccggtct tgcttatttt ccggggcttt cctatgggtg ggcaaataaa tacttacatt
     2821 gataccacca tttacttctg ccaattctcg gatgttaata ttagccatct aatcctcctt
     2881 ctttgtttta agcttccttg ctacttatct tctattatac cctttaaggg tagaaattcc
     2941 ctttatcaga ggtaatttgt tatttaagct tttagtagga agaaattcga aattaattta
     3001 atcctacttt ctaattctat tatagaacaa atacgataac aattcacaaa tgttataaaa
     3061 atatcagtat cttttcttta caaaggccag accctctagg aggacttttt aaaagatgaa
     3121 attctagcaa gagtgattta caacttgcaa taagaagaaa aaaggggctt atcttcttct
     3181 ttataaaata aaagtctatt ggaatatttt tttaaaagat tgatttgaat caaggtttaa
     3241 tccccggtaa ctagctatga tatacctata gagattaagt agtctatata gcaataacta
     3301 gcactgctga ttaggctaag aactagacca tagtgagtaa aaaaaagaga gtcttcttac
     3361 taacaaatat agttaaagaa aaaaagaaag gacaataata atgaaaaact cagaaatttt
     3421 aaaagaaatg attaaaaagc tccacaaggg agccagtgta gatgatctta agggagaatt
     3481 tagaaagcat tttaaggatg tgccggccca ggaaatagct gatgcagaaa aagaacttat
     3541 ggaggagggc tacaccgtag aagaaatcca atcactttgt gatgtccatg ccagcttgtt
     3601 tgaaaatcgt gttcaaattg aggttaaatc aaaagagcta ggccatcccc tccatatttt
     3661 taaagaagaa aacaagggcc tacttgactt tcttgctaat aagtttgatc ccttatttga
     3721 agactataaa aaagatcccc agggcaaaaa ggacagcatg ctttatggac taaaagaact
     3781 ttcaaaagta gatacccact atagtagaaa agaaaatctt cttttcccct ttttagaaaa
     3841 agctggtgtt acagcacctc ctaaggtaat gtggggtgtt gatgatgaga taagagcctt
     3901 cttaaaggaa gcactcgcag ctgttaagga agaaaacgtt gaaaatgcaa caaagtcggt
     3961 gataagtgcc agagaacaaa taaaatcgat gattacgaaa gaaaatgata ttcttatgcc
     4021 ccttctttct aagcatgtgg atgatgaggg ctggaaacta gtagcccagg aaagcctaca
     4081 ctttggctat gtatttaccg gagacagaga aaacgcaagt ccctctgatg cagccgcttg
     4141 gctaaaaaaa gggtccacag taactcagcc aagaaatgat gcccccatcc agctagccag
     4201 tggtttcttt acggtaaatg aactcaccac actttttaat accctacctt gtgatattac
     4261 ctttgttggc gccgatgata aggtgcatta ttttagtgaa tctaaggata gggtctttcc
     4321 aagaacccgc acaattattg gacgagatgt agaggattgc catccaccaa aaagtcttga
     4381 tagggtaagt gatattgtcg atgactttaa agcgggtaaa aaagatacag agtcattctg
     4441 gattaagatg ggagaaaaat ttattctaat taggtactac gccctgagag atgaagaggg
     4501 cagctatctt ggggtcttag aagtatctga agatatcgca ccccttagag cccttgaagg
     4561 ggagaaaaga ctctaaggta agacaccttt aaataaatta gtagaggtag tagataaaaa
     4621 agctggtata aataccggct tttttacatt aggccaggac aaaaacctgg tggactaaga
     4681 ctgctctagt agaaaaactt agggtctata gaagtaaagg aaaagatttt ctcttgatta
     4741 ttattagagg cagcactggc ccctgctatt tcctaaatta ttatagcaag gctaagatct
     4801 gcccttagtt tttgacgcat tatgtggtaa tctaacacta taaatagatc ttaagaggct
     4861 aggaccttta cttatactag gaggacacaa aaaacagtaa cacgttctgg ttaatttatt
     4921 gacaaggcat aaactaactg ttagcgtagg tatatgcata ataatgaaca ggataaaaaa
     4981 ggagctaatt atgaataaaa aaagatggat tgccgtggct ttatcagttc ttgtctttat
     5041 ttcttccttt gtcgtatctt ctaaaggaaa atcaaaaatc atagaaaaag aaactacatc
     5101 ccttttaaaa gaacttatga gcgaggaatt atctaaaact gtactgcgcc ctggaaatgc
     5161 aaatcagcgc atagctgtct tacatattcg aggtacaatt ttaaatatgg aaggaaactg
     5221 gctaaacgct tcgcagtaca atcacaatct caccataaat tcacttgatt ccataagaaa
     5281 tgatgaaaca gtcaagggga ttattatccg catagatagt cctggtggtg gagtatacga
     5341 aagcgcccag ctaagtgata agataggcga aataaaaagg acaagaaata ttccagttta
     5401 tgtagttatg gaaagcatgg cagcgagtgg gggctactat atagcggcag gggctgatca
     5461 aatctatgca acagaagaaa ccttgactgg ctccattggt gtaattatgt ctgggatgaa
     5521 ctattctgga ctgatggaca aattaggtgt ggaagatatg acaattaaaa gcggggaaat
     5581 gaaggatgta ggttccctta cccgtgagac gacaaaaaaa gatttagaag tattgcaaga
     5641 actagtagac aatatgtatg agcgctttgt tgatgtagtg gtagagggta gagacatgga
     5701 tagggcaaaa gtgtataaac tagctgatgg taggatttac gatggagccc aggctaaaaa
     5761 taatggtctt gttgataaac taggctatta tgatgaggct ttagccgatt ttgagaaggc
     5821 ctatggtttg caagaagcgg aagtctttag ctatgacaat atgggtctag ataaaatcag
     5881 tcaactcttt atgaaaagtg caaatttatt taatccaaga aaagaaataa atgtaggaaa
     5941 cattgagcta ccagcaagct ggaaagaaag ttctggattt atgtatatct atggaggcta
     6001 ttagtatgga aaaatatcat aactacccat caatagtata tgcaggcttt tggataagat
     6061 tttttgccta ttgtacggat cttattatga ttggaagcct taataggatg ctacttttct
     6121 ttcttgagga gggaagagtt aaaacgatag ctagcctagg ggtatttcta ctttactttc
     6181 tgctaatgac aaagcttaat aatgggcaaa ctatagggaa gatgatcttt ggtcttcgtc
     6241 tagtaagctt tagccaggaa aaactaagct ggacaacagt agtggtaagg gaaatctttg
     6301 gacgatactt gcttaaaagc tatgcaataa tttatcttat cactgccttt tcagaaaaaa
     6361 agcagcatgg gatagatatg ctccttgata caacggtaat ttcagaaaat agcttgcaac
     6421 tatataaaga aataccaata aatataggag aagaggtaaa aagggaagaa cttgcggctt
     6481 attagaagct aaagagactc atgctagtag atactaaat
//
LOCUS       DASRNA010000215         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_216033,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000215 DASRNA010000000
VERSION     DASRNA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216033"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1688)
                     /locus_tag="VFD08_06040"
     CDS             complement(45..1688)
                     /locus_tag="VFD08_06040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK10614.1"
                     /translation="MRFEEFSLSKEILKGIKGMGFEETSPIQASSIEPLLEGKDIIGQ
                     AQTGTGKTAAFGIPMLELINKGGQVQGLVLVPTRELAIQVSNELAELGKYKSGVQVLP
                     VYGGADIRRQLKALKRGVQIVVGTPGRLMDHLRRKSLKLDNLKMVVLDEADEMFAMGF
                     RDDMKTIMDQTNENKQTCFFSATMGSDIQNFSKLYQKEPLIIRVKHKELIVENIQQHY
                     LEMTGQMKTEILSRIIDINEPELAIVFCNTKRMVDNLVGELSSRGYSADALHGDLKQN
                     QRDMVMNKFRKGTIDILVATDVAARGIDVGNVDIVVNYDLPQDEEYYVHRIGRTARAG
                     KEGKSFTFVVGRDAHKLQEIIKYTKAKMEYMELPTIDDMRETKKDHLKDALVEQLEKK
                     ENLKKYNSIVDSLLQEEYGPVEIAAAMMKLFDENQKMRDYEKLDRVDYGKKYVMPRST
                     RSSRDKSPKRGKNPKRSRRKGSRIFIDKGERDGLNPAAVFAAIKSESDIPSHLIGDIA
                     IMKNFSFVEIPKDYMDQTIKSLDGKKIKNKKVRVEVAGN"
BASE COUNT          548 a          472 c          340 g          792 t
ORIGIN      
        1 attttacttt tatcgcccta gattatctta ggcttaaaga aaaattaatt accagcaact
       61 tccactctaa cttttttgtt ctttattttc tttccatcta agcttttaat ggtttgatcc
      121 atataatctt taggaatttc tacaaaggaa aagtttttca taatggcaat atccccaata
      181 agatgtgagg gtatatcgct ctcactctta attgcagcaa agacagctgc agggttaagt
      241 ccatctcttt cacccttatc aataaaaatc cgactacctt tgcgtctaga tcttttaggg
      301 tttttgcctc tttttgggct tttgtctctt gatgatctgg ttgatcttgg cattacgtac
      361 tttttgccat aatctaccct atctagtttt tcatagtctc tcattttttg attttcatca
      421 aatagcttca tcattgctgc tgctatttcc acaggaccat attcttcctg gagtaagcta
      481 tcaacaattg aattatattt cttaaggttt tctttctttt ctaactgctc aaccaaggca
      541 tcctttaagt gatccttctt ggtttctcgc atatcatcaa tggtaggaag ttccatatat
      601 tccatttttg ccttggtgta tttgatgatt tcttgcagtt tatgggcgtc tcttcctaca
      661 acaaaggtaa atgatttgcc ttccttgccc gccctggccg tacggccaat tctgtggaca
      721 tagtattctt catcctgggg caaatcgtag tttacaacga tatcaacatt ccccacatcg
      781 attcctcgag ccgctacatc tgtagccacc agtatatcga ttgtcccttt acggaattta
      841 ttcattacca tatctctttg gttttgtttt aaatcaccgt gaagagcatc tgcactatat
      901 cctctagagc ttagctcacc aactaagtta tcaaccattc tctttgtatt acagaaaaca
      961 atggccagct ctggctcatt gatatcaata attctactta aaatttccgt tttcatctgg
     1021 ccagtcattt ccagataatg ttgctgaatg ttttcaacaa tgagttcctt gtgttttacc
     1081 ctaattataa gaggctcttt ttggtacaac ttagaaaagt tttgaatgtc gctgcccatg
     1141 gttgctgaaa agaaacaggt ttgtttattt tcattggttt ggtccatgat tgttttcata
     1201 tcatccctaa aacccatagc aaacatctca tctgcttcgt ctaagactac catctttaag
     1261 ttgtctaatt taaggctttt tcttcgtaag tggtccataa gacggcctgg ggtgcctact
     1321 acgatctgga ctcccctttt tagggccttt agttgtcttc taatatcagc tccaccatag
     1381 actggtagga cctggacacc gcttttgtac ttgcccagtt ctgctagttc gttggatact
     1441 tgtatggcta gttctctggt tggtactaaa acaagtccct ggacttgtcc acctttattt
     1501 atcagctcta gcataggtat gccaaaggct gccgttttcc ctgttcctgt ttgtgcctgt
     1561 ccgataatgt cttttccttc taacaaaggc tcaatactac tagcctgtat ggggcttgtt
     1621 tcttcaaatc ccattccctt gattcccttt aatatctctt tagataggga aaattcttca
     1681 aatctcaata tgtctcctct ttcacataaa aaaaaccgag acgaactcgg tttccaagtt
     1741 ttatatcttt tcaaacattc taacacatcc cttgacaaat agcaaagatt caaccttata
     1801 gagatgaatt catttttgtc acactcctgc ttttaccata gatgcctttt taatcctaag
     1861 cagctaatct tttactcgaa tcgatctagg agggaaaatg tcaattaaaa aacgactgcc
     1921 ttatgtaaag ccctcccctg tttttttggg agaaattttt tagtcctatc tgcctattga
     1981 ttacagcaaa gaatgaacaa tgagaactta taaaaaaagc cctagttacc ctagggattt
     2041 ctagggctca tattaggtca atttatttta aagatatcat aaagatgctt tctttttacc
     2101 gcgtcgtttt cggatcataa aattcttttt ttctgatctc ataggcttgc at
//
LOCUS       DASRNA010000216         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_216260,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000216 DASRNA010000000
VERSION     DASRNA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216260"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFD08_06045"
     CDS             <1..445
                     /locus_tag="VFD08_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10615.1"
                     /translation="FDYIKVYLRALLRDKEKNPPSGSVCASGETIRSMEDFLSKLSLR
                     VYYKGRPIYDLSAKELERLEENIYLGTLKKGESIKLQVELEVPLELGNKYMERIAEVD
                     WVFLVEAFDKDGSQETISNRDALIQTGQKTSRFTYLLEWVFFSFL"
     gene            complement(634..2355)
                     /locus_tag="VFD08_06050"
     CDS             complement(634..2355)
                     /locus_tag="VFD08_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZK10616.1"
                     /translation="MYLKKDKRPNDRVYLSIVKSFRDPITKKSRQRAVLSVGFLDELI
                     DQYPDPIAHFEELARQMTEQENASQQPLTFSFDKEELLKRDDRLRKKLGFLPLSFVYH
                     KLELDRFLIHKQRNLNIEYSLNQVLQSLVYLRILEPSSKKKTYELLDSYFFERNFTAA
                     DIYRSLDRFSSYRYEILRHLNEMVTMKYGRNTKRAYYDVTNYYFEITKEDAFRKKGVS
                     KEHRPNPLVQMGLLLDGDGIPLSYQLFEGSKIDSQTYMPFIHELKDEFNLKRVIVVAD
                     KGINTGENIAYNILGNNGYIFSQTIRGATNEIKDYVLDELGYSHHESGFKKKSRIVDT
                     KIWVLDELGKRKSVEIEQKQVVFYRPDYDAKAKHERYKLIEKAKKNLSKKGHLRTTGS
                     YKYIAKDRIDKKTGEVSKEKDHYSIDLDKVFEEEKYDGYYLIVTSELDTSDDEIIQAY
                     RGLWKIEESFKVTKTSLKTRPVWLSRKTSIEAHFLTCFISLLLLRLIEKELKAGDGDF
                     FFSPAGLIDSLKNYSTTYLDENYYMFDYYDDNLEKMAQMMGMDLAKRFRTRQEIKNMI
                     AKAKVQL"
BASE COUNT          704 a          497 c          530 g          844 t
ORIGIN      
        1 ctttgattat atcaaagtct atttaagagc tctcttgcgt gataaagaaa aaaaccctcc
       61 tagtggtagt gtgtgcgcct ctggtgagac aataaggtcc atggaagatt ttttatcaaa
      121 gctttcactg agggtctatt ataagggaag gcctatttat gatctttctg ccaaggagct
      181 agaaaggcta gaagaaaaca tctaccttgg gacacttaaa aaaggcgagt ccattaaatt
      241 acaggttgaa ttagaagtac cccttgaact aggaaataag tatatggagc gcatagcaga
      301 ggtagattgg gtttttcttg tagaagcctt tgataaagac ggtagtcaag aaaccatttc
      361 taatagagat gctttaatac aaacaggtca aaaaaccagt cgttttacct atttgctgga
      421 atgggttttt ttctcctttt tataggtgta atgatccttt ctagaaaaaa aaggagagaa
      481 gatggctaaa aaagcaagat aggatttata atttttgctc tagtagaata gcttttatat
      541 cttgactttg acagcttaca gcctaaaaaa gctcccttaa cccttggcat tactgggttt
      601 aaggaagctt ttctttttca gaaagatgtg ctactacagc tggacttttg cttttgctat
      661 catatttttt atctcttgtc tggttctgaa gcgttttgcc agatccatac ccatcatctg
      721 agccatcttt tcaaggttat cgtcgtagta atcaaacata tagtagttct cgtccaggta
      781 agtggtcgag tagtttttta gtgaatctat aagcccagcg ggggagaaaa agaagtctcc
      841 gtccccggct tttagttctt tttctatgag ccgaagtagt aaaagggata taaagcaggt
      901 gagaaagtgt gcctctatgc tggtttttct agataaccat acaggccttg tcttaagcga
      961 ggtctttgtt accttaaagg attcttctat cttccatagg cccctatagg cctgtattat
     1021 ctcatcatcg cttgtatcga gttcactggt aacaatcaag tagtagccat catacttctc
     1081 ttcctcgaat actttatcta gatcgatgga gtaatgatct ttttctttac tcacttcccc
     1141 tgtcttttta tctatccgat ccttggcgat gtacttataa gagccagttg tcctaaggtg
     1201 gcccttttta gacagattct tctttgcctt ttctatgagt ttgtatcttt cgtgttttgc
     1261 cttggcgtcg taatccggac gataaaagac gacctgtttt tgctctattt caacggattt
     1321 tcttttgccc aactcatcga gtacccagat ctttgtatca acgatacggg attttttctt
     1381 aaagccgctt tcatggtggc tatagcccaa ctcatccagg acatagtcct tgatttcatt
     1441 tgtggctccc cttatcgttt gggagaagat atatccattg ttacccagga tgttataggc
     1501 tatgttttcg cccgtattga ttcccttatc agccacgacg atgactcttt ttagattaaa
     1561 ctcatccttt aattcatgga taaagggcat gtaggtttgg gagtctattt ttgagccttc
     1621 aaagagctga tagcttaggg gaatgccatc cccatccagg agcagtccca tttggacgag
     1681 ggggtttggc ctatgctcct tagaaacccc tttttttcta aaggcatcct cttttgtaat
     1741 ctcaaagtag tagttggtta catcatagta ggctcttttc gtgtttctgc cgtacttcat
     1801 cgtcaccatc tcgttgagat gcctgagtat ctcgtagcgg taggaactaa atcggtccag
     1861 acttctgtag atgtctgccg cagtaaagtt cctttcaaaa aagtagctat ctagtaactc
     1921 gtaggttttt ttcttactag aaggttctag gattcttaga tagaccagtg actggaggac
     1981 ctgatttaaa gagtattcaa tgtttaaatt tctttgttta tgaataagaa agcggtccaa
     2041 ctctagctta tgatagacaa aagaaagagg aagaaagcct agctttttac gtagcctatc
     2101 atcccgcttt aggagctcct ctttatcaaa gctaaaagtg aggggctgct gggacgcatt
     2161 ctcctgctca gtcatttgcc tggctagctc ctcgaagtgg gcgattggat cgggatactg
     2221 atctatcagt tcatcaagaa accccacact aagtacagct ctttgcctag actttttggt
     2281 aatgggatct ctaaaggatt tgacgatgga taggtagact ctatcgtttg gacgcttgtc
     2341 tttttttaga tacataatgc acctccgctt tttataatta taccatagag tcacatagaa
     2401 gtacagcaaa aagaattaaa tttgacaaga aaaaaagctg caaagactgg cagcgacgaa
     2461 gcaaatagat ttgaagatta gtaaatcggt gttaaactag ggaaaaccag gtcagttttc
     2521 aaagtaaagg caacactggg ttgaaatcat ctctgtttaa tgaactactt gcatt
//
LOCUS       DASRNA010000217         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_142457,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000217 DASRNA010000000
VERSION     DASRNA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142457"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /gene="lepA"
                     /locus_tag="VFD08_06055"
     CDS             <1..1768
                     /gene="lepA"
                     /locus_tag="VFD08_06055"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZK10617.1"
                     /translation="AHIDHGKSTLADRLIEISGALTQREMKSQVLDNMDLERERGITI
                     KLQTSRLYYVAEDGLEYVFNLIDTPGHVDFTYEVSRSLAACEGALLIVDAAQGVEAQT
                     IANVYLADAEGLEIIPVINKIDLPSARVDEIKEEIEEVIGIEAHDAPLISAKTGLNIR
                     DVFEAVIKKVPAPQGDLKAPLKALVFDSFYDPYKGVIPLIRIFDGEIKKGDEVYMMAS
                     KESFEVTEVGIFSPVMTKVDSLKAGDVGYLCASIKDVRHVSVGETVTHTNRKVEKALP
                     GYKKALPMVFCGLYPAEGEDYENINDALEKLQLNDAALTFEPETSVALGFGYRCGFLG
                     LLHLEIVQERLEREFDLDLIATAPSVIYKVVTTSDETLFIQNPSNLPEPTKIEHFYEP
                     IVKANIFVPKEYVGNVMELCQQRRGEFQHMEYLDERRVLLDYYLPLNEIIYDFFDALK
                     SKTRGYGSFDYEFYDYVESDLIKMDILINKEPIDAFSMIVHREKSVPRGRQIVERLKD
                     VIPRQQFPIPIQSAIGNNIIARETIRALRKDVLAKCYGGDISRKKKLLEKQKVGKKRM
                     RQLGSVEIPQQAFLSVLKFDEE"
     gene            1758..2480
                     /locus_tag="VFD08_06060"
     CDS             1758..2480
                     /locus_tag="VFD08_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK10618.1"
                     /translation="MKNNLALYIHIPFCKKKCNYCDFLSFSSNASTRELYIETLLKEM
                     DLYKPLFKKKKISSLYFGGGTPSMLSAWELDKLLQGVFARVEPEGELDFEMNPDDVTP
                     EILKILQNYGDFRISLGVQSFDNNLLKLFGRNHGVKEIFASIDMIRRLGFDNLSLDLI
                     YAIEERFTMADNLTAVGQVLPEHLSCYALELHPKRPLARLIKEPSDELYLRDWSRLKE
                     GLKSLGYQRYEISSFARQKKTG"
     gene            2488..2853
                     /locus_tag="VFD08_06065"
     CDS             2488..2853
                     /locus_tag="VFD08_06065"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10619.1"
                     /translation="MNYWRGGDYLGLGLGAHGFLTPMRYSNERNLEKYLKRVSEKELP
                     IDEQLSLCKKELRFENFMLGLRLVEGIDLEPLYPLSIQEEKALVKHKKNKLLQQKGKT
                     LSMTEEGFDLFNYVLVDFM"
     gene            2908..>3867
                     /locus_tag="VFD08_06070"
     CDS             2908..>3867
                     /locus_tag="VFD08_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HZK10620.1"
                     /translation="MYKHTIEERLDELSSSRRGLSEEERALRHETYGYNELSNKENRS
                     FFSFYGESLRLPILRILLLIGLLFAIVQRFEEALILFGIFLLRSITEGLKSYRAQRAR
                     REFFKQNTRNCKVLQGEKIIELPIRELVPGDIVLLDEGEIIPADGRLIEGDNLWIDES
                     RLTGEDKPFCKDVSFITEENIPLAERMNMVYASTLVCEGQGLMIVTSTGMETEFGKVA
                     FMLYKDEVIESPLDIKLLTMTKYTKIFIVLLSIGFAIYSFLMGRTIEEVVFVSIALVL
                     AATPNTLNYLVFHLMSFGALRFSKNGVTLKRLNDIEKLSSISVV"
BASE COUNT         1225 a          616 c          831 g         1195 t
ORIGIN      
        1 tgcccatatt gatcatggga agagtaccct agcagatcgt cttattgaga taagcggcgc
       61 tttaacccag cgtgagatga aaagtcaagt tctcgataat atggatttgg agcgtgagag
      121 gggaataacg ataaaactgc agacatcccg cttgtattat gtagccgagg atggtttgga
      181 gtatgttttt aatttaattg acacacctgg acatgtagat tttacttatg aggtttctcg
      241 cagtcttgca gcttgtgaag gagctttact tattgtcgat gccgcccagg gagtcgaagc
      301 acagactatt gcaaatgttt atttggctga tgcagagggg cttgaaatta taccagttat
      361 aaacaaaatc gacctgccct cagctagggt tgatgaaatc aaagaagaaa tcgaagaagt
      421 tattggcata gaagcccatg acgcccctct aattagtgct aaaacaggcc ttaatataag
      481 agacgttttt gaagctgtaa ttaaaaaagt gccggcacct cagggtgatt tgaaagcacc
      541 cttaaaggcc ttggtttttg attcttttta tgatccatat aaaggagtca ttcccctaat
      601 cagaattttt gatggagaaa ttaaaaaagg tgatgaggtt tatatgatgg cttcaaaaga
      661 gagttttgaa gtaaccgaag tgggcatttt tagtccagta atgacaaaag tggattcttt
      721 aaaagctggc gatgttggct atctttgcgc tagcattaaa gatgttcgcc atgttagtgt
      781 gggcgaaacc gttacccaca ccaatcgtaa ggtagaaaag gcgcttccgg gttataaaaa
      841 agccctaccc atggtatttt gtggtcttta tcccgctgaa ggggaggact atgaaaatat
      901 caatgatgct ttggaaaagc ttcaacttaa tgatgcagca ttgacctttg agcctgagac
      961 aagtgttgcc ctaggctttg gatatcgctg tggatttttg ggtttgcttc atttagagat
     1021 tgtgcaagag cgccttgaaa gagagtttga tcttgatctt attgctactg ctcccagcgt
     1081 tatctacaaa gtagttacaa cctcagatga gacgctattt atacaaaacc ccagtaattt
     1141 accagagcca acaaagattg aacactttta tgagcctatt gttaaagcca atatatttgt
     1201 tccaaaggaa tatgtgggta atgttatgga gctttgtcaa caaagacgag gggaatttca
     1261 acatatggag tatctagatg agcgcagagt tcttttggat tattatctac cactaaatga
     1321 aattatatat gatttttttg atgctctaaa aagtaaaact aggggttatg gttcttttga
     1381 ttatgaattt tatgattatg tagaaagtga tttaataaaa atggatattc ttattaacaa
     1441 agaacccatt gatgctttta gtatgattgt acatcgagag aaatctgtac ctcgaggaag
     1501 acagattgtt gaacgcttaa aagatgtgat tccacgtcaa caatttccca ttcctattca
     1561 atcagccatt ggcaataata ttatagctag agagaccatt agggccctta gaaaagatgt
     1621 tcttgccaaa tgctatgggg gagatattag ccgaaagaaa aaacttctgg aaaagcaaaa
     1681 ggtaggaaag aaaagaatgc gtcaattggg tagtgttgaa attccccagc aggcttttct
     1741 tagtgttctt aaatttgatg aagaataatc ttgcccttta tattcatata cctttttgta
     1801 aaaaaaagtg caattattgt gactttttaa gcttttcatc aaatgcatct acacgggaat
     1861 tatatattga aacactgtta aaagaaatgg atctctataa gcctcttttt aaaaaaaaga
     1921 agattagtag tctctacttt gggggtggca cacccagcat gcttagtgct tgggagttag
     1981 acaaacttct ccaaggagtt tttgctagag tagagcctga gggtgaatta gattttgaaa
     2041 tgaatccaga tgatgtaact cctgaaatat tgaagatttt acaaaattat ggggatttta
     2101 gaattagcct tggagttcaa agttttgata acaatctatt aaagcttttt ggtagaaacc
     2161 atggagttaa ggaaattttc gctagtattg atatgataag aagattgggt tttgacaatc
     2221 tttctttgga tcttatttac gccatagaag aaagatttac tatggcagac aatctcacgg
     2281 cagtaggaca agtcctgcca gaacatctat catgctacgc actagaactt catcccaaac
     2341 gccctttggc aaggctaatt aaagaaccta gcgatgaact atatcttaga gattggtcaa
     2401 ggttaaaaga aggcttaaaa tcacttggct accagagata tgaaatttcg agttttgcca
     2461 gacaaaaaaa aacagggtaa acataacttg aattattggc gaggcggaga ctatctaggt
     2521 ttgggtttag gagcccatgg ctttttaacc cctatgcgtt atagcaatga aagaaatctt
     2581 gaaaaatacc ttaaaagggt atctgaaaaa gagcttccta ttgatgagca gttgagtctt
     2641 tgcaagaaag aacttcgttt tgagaatttt atgctaggtc ttcgtttagt agaaggaata
     2701 gatcttgaac ctttataccc cctttctatt caggaagaaa aagccctagt aaaacacaaa
     2761 aagaataagc ttttgcagca aaagggaaaa actctaagta tgacagagga aggttttgat
     2821 ctgtttaact atgtgttggt tgattttatg taaagtagcc tttaaaaatc agctgtgata
     2881 caatggccta acagaattgg aggttacatg tacaaacata ctatcgaaga aaggcttgat
     2941 gagctaagtt catcaagacg tgggctctct gaagaagaac gagctctaag acacgaaact
     3001 tatggatata atgagctttc aaacaaagaa aatcgctcgt tttttagttt ttatggtgaa
     3061 agtttacgct tacctattct gagaatactc ttattgatag ggttgctttt tgctattgta
     3121 cagcgttttg aagaagctct tatcttattt gggatttttc ttcttcgtag cattacagaa
     3181 ggtctaaaaa gttatcgagc tcaaagggcc cgcagggaat ttttcaaaca aaatacaagg
     3241 aattgtaaag ttcttcaggg agaaaagatt atagagcttc ccataagaga gcttgtgcct
     3301 ggagatattg ttctactaga tgaaggtgaa attattccag cagatggacg cttaatagag
     3361 ggtgataatc tgtggattga tgaatctcgc ctaacgggag aggacaagcc cttctgtaaa
     3421 gatgttagct ttatcaccga agaaaatatt cctttggccg aaagaatgaa tatggtttat
     3481 gcctcaactc ttgtttgtga gggccagggc ctaatgattg tcacatcaac aggcatggaa
     3541 actgaattcg gtaaagttgc ctttatgctc tataaagatg aggtaattga gtctcctctt
     3601 gatattaaat tgttgactat gacaaaatat acaaagatat ttatcgtctt actttctatt
     3661 ggctttgcga tttattcttt tttaatgggg aggacaatcg aagaagtggt ttttgtgtca
     3721 atagccctag ttttggccgc cacacctaat accctaaatt atttggtctt ccatcttatg
     3781 tcctttggtg ctttgcgctt ttcaaagaat ggagttactt taaaaagact caatgatatt
     3841 gaaaaactaa gtagtatatc ggtcgtt
//
LOCUS       DASRNA010000218         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_743058,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000218 DASRNA010000000
VERSION     DASRNA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743058"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1349)
                     /gene="uraA"
                     /locus_tag="VFD08_06075"
     CDS             complement(87..1349)
                     /gene="uraA"
                     /locus_tag="VFD08_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002675699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HZK10621.1"
                     /translation="MEKKKIYQIEEKVPPALLFPLSLQHTFAMFGASVLVPIIFQIDP
                     GIVLFMNGIGTLFFIFVTKGKAPAYLGSSFAFLGPASVIIASLGFNYAQGAFVVTGIL
                     GCILAYIIYRYGTDWIDIVLPPAAMGAVVALIGFELTSLTVQGGAIGANIMVEGATLA
                     DLSVFLITLGVAVFGTVLFRGFFSTIPILIAIVIGYLSAWAFGMVDFSSVMEASLFTI
                     PNFQLPKFNMGAIITMLPVLLVITSEHISHQVVTSNIVGKDLLKDPGLHRTIFADNFS
                     TALSGLVGGVPTTTYGENIGVMAVTGIYSVQVIAGAAIISIIMAFLGPLAAIIATIPG
                     NVIGGVTFLLYGMIGASGIRLLVDSKVDYSKSKNLILTSVVFTTGLSGVAINFGSIQL
                     SGMVLSSVVAVFISLVFHLFERTGILNE"
     gene            complement(1426..1968)
                     /locus_tag="VFD08_06080"
     CDS             complement(1426..1968)
                     /locus_tag="VFD08_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZK10622.1"
                     /translation="MKKTTSVVLVLLILVFLVCELRPREIIEPLYYTKEIREICEIHD
                     IDPFIIAAVARTESSWVSDATSRVGAKGLMQIMPETALYLAEERGLYLALEELNDPRV
                     SLDYGAYYLKKLYGQFKNWDTVFAAYNAGPTQVEAWLNDSNYSNKKELTYIPYDETRH
                     YVEKVNHFLEAFKKSYKRFP"
     gene            2074..>2271
                     /locus_tag="VFD08_06085"
     CDS             2074..>2271
                     /locus_tag="VFD08_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10623.1"
                     /translation="MNRFEAFFKELEERSSGMRKNKSWLSFLLTFVFLALYFYFTLPS
                     LSYQSPSLYSFLLIGLLVFTGL"
BASE COUNT          738 a          475 c          412 g          646 t
ORIGIN      
        1 atctaaagca aggggcgcct tgttacatat gtagaaatct tagctagttt ttcgtcaaat
       61 cgcccctagg ctcttaggaa aagttcttat tcattgagaa ttcccgtgcg ctcaaaaaga
      121 tgaaaaacaa gactaataaa aactgccact acagaagata aaaccatacc tgaaagttga
      181 atgcttccaa agtttatagc aacaccacta aggcctgtag taaagacaac ggatgttaga
      241 ataaggtttt tgctttttga ataatctacc ttggaatcta caagaagccg gattcctgag
      301 gcaccaatca tcccatagag aagaaaagtt actcctccta taacatttcc tggaatcgtt
      361 gcgataatag cagctagtgg gccaagaaaa gccataataa tactaataat ggccgctcca
      421 gcaataacct ggacagaata aatacccgtt actgccataa caccgatatt ttcaccgtag
      481 gttgtggtag gaaccccgcc aacaagacca ctaagagcag tagaaaagtt atctgcaaat
      541 atcgtccggt gaaggcccgg gtctttaagt aaatctttgc ccacaatatt gctagttaca
      601 acttggtggg agatatgttc actggtaata acaagaagta ctggcaacat ggttataatt
      661 gcacccatat taaattttgg tagctggaaa ttcgggatgg taaaaagact cgcttccata
      721 actgagctaa agtccaccat accaaaggcc caggcactaa gatatcctat tacaatagca
      781 ataagaattg gaatggtaga aaaaaagcct cgaaacaaaa ccgttccaaa aactgccaca
      841 cctagtgtga ttaaaaaaac actcaaatct gcaagggtgg ctccttcaac cataatattt
      901 gccccaatcg cccctccttg aactgtaaga gaggtaagct caaagcctat aagagcaaca
      961 accgctccca tagcagccgg gggaagcaca atatcaatcc agtcggttcc atagcgataa
     1021 ataatatagg ccaagataca acctaaaatc cctgtgacga caaaggcccc ttgagcatag
     1081 ttaaatccta aacttgcaat aattacactg gctggtccta gaaaggcaaa actactgcca
     1141 agataagctg gagcctttcc cttggtaaca aagataaaaa acaaggttcc tatcccgttc
     1201 ataaaaagta caatacccgg atcaatttga aatataatgg gaactagtac tgaagcccca
     1261 aacatagcaa atgtatgttg caagcttaga ggaaaaagca gtgccggtgg taccttttct
     1321 tctatttggt aaattttctt cttttccata aatcctcctt ttcctataca gtatacagga
     1381 ggaaaaatac atcctcaaga agaaaagaaa aaactcgtct ttatattagg gaaatctctt
     1441 ataacttttc ttaaaggcct ctaaaaagtg attgaccttt tctacataat gtcttgtttc
     1501 atcataaggt atataagtaa gttctttttt atttgaataa ttggaatcat taagccaagc
     1561 ctccacttga gttgggccag cattataggc agcaaatact gtatcccagt ttttaaattg
     1621 cccatagagt tttttcaagt aataggcccc ataatcaaga ctcaccctag gatcatttag
     1681 ttcttctaag gctaaataaa gaccccgctc ttctgctaga tatagggccg tttcgggcat
     1741 aatctgcata agtccctttg cacccactcg acttgttgca tcagaaaccc agctgctctc
     1801 tgtgcgagcc acagctgcta taataaaggg atctatgtca tggatttcac aaatttccct
     1861 gatttctttg gtataataaa gaggttcaat aatttctcgg ggccttagtt cacatactaa
     1921 aaagaccagt ataagaagga caaggaccac tgatgttgtt tttttcatct tttctcctag
     1981 gatgtaacta taacagaaga gatgtttttt tgaaagttct ggtatttttg tcatattagg
     2041 gatatctata gactagtcgt gaaaggagtc gttatgaatc gttttgaagc attttttaaa
     2101 gagctcgagg aaagatcctc gggaatgagg aaaaacaaaa gttggctaag ctttttactt
     2161 acctttgttt tccttgcctt gtatttttac tttaccctac cctccctatc ttatcaatca
     2221 cccagccttt atagtttcct tttaataggg cttttggtct ttactggtct t
//
LOCUS       DASRNA010000219         8032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_511058,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000219 DASRNA010000000
VERSION     DASRNA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511058"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1039)
                     /gene="rarD"
                     /locus_tag="VFD08_06090"
     CDS             complement(182..1039)
                     /gene="rarD"
                     /locus_tag="VFD08_06090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HZK10624.1"
                     /translation="MAVLAQVIWGLLAIYWKVLDFLPAYQILVHRMLWSVISLHILIY
                     FLKGRGYSKKLLADRSHRKISLLGGVFITLNWFLYIWSINSGHVLEASLGYYILPLIT
                     GLIGLLLGEKLNQKQWLANGFAFVGVLISTLGLGRVPWVALALALTFAIYTYLKKQSP
                     LDAIDSLYTETIFMAPLALIYFLYSEVAGMGISGNFPWTVWLLLATTGVVTALPLLLF
                     GYGARKLPFKIISFIQYLSPSFTFLLGIFLFKEDFDLMRLFSFVFIWLGLGIFTLDQI
                     KNKEKKISA"
     gene            complement(1171..1404)
                     /locus_tag="VFD08_06095"
     CDS             complement(1171..1404)
                     /locus_tag="VFD08_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10625.1"
                     /translation="MGNYRKGNPMNQESGSYTIELTVLILAIFFVFVLFFAVAGDAFE
                     GLYIPSKEPSCYEEAFSKKIEDLRHEKVLREQR"
     gene            complement(1374..1994)
                     /gene="tmk"
                     /locus_tag="VFD08_06100"
     CDS             complement(1374..1994)
                     /gene="tmk"
                     /locus_tag="VFD08_06100"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZK10626.1"
                     /translation="MEGKWISFEGGEGSGKTTLIKGLVEKLEERNLPYLLTREPGGVP
                     VAEKVREILLEKTDRPMDAMTEAILFAASRRQHLVEKVLPALTEGKIVLMDRYVDSSI
                     VYQGYARGLGMEKIKALNEFATDGHLPIITFYLDLAPQKGLERIIENKRLMNRLDDEN
                     LDFHKKIREGYLLLCEKEDRLRCLDADKRPQQLLEEVWEIIAKEIL"
     gene            complement(2004..3809)
                     /gene="uvrC"
                     /locus_tag="VFD08_06105"
     CDS             complement(2004..3809)
                     /gene="uvrC"
                     /locus_tag="VFD08_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZK10627.1"
                     /translation="MYKVPAHIREQLNTVPQSPGVYSMKDALGKIIYVGKAKKLKNRL
                     SSYFRGVDSHPVKTKALVVNTKEFEYILTNSEEEALLLEANLIKAHRPKFNVLLKDDK
                     SYPYVLLTKEKYPRLFKVRGLDSRGTCFGPYTGDFDVNNVLEALHNIYPIRKCHLPLN
                     KITRPCLYYHMDKCLAPCAFSHVKEDYDRAVEEIQGFFKEDRKAFIGILEKKRDEAAQ
                     RLDFEGAIVFREQMDAAKRLSLHEQLSQLRKDDIDYISFSEKEQRLCITLFVRRHGKI
                     IDRENHIFDNLLEKDRGETLQEFILQYYEEASFIPGELVVETLSEIGLVKEYLKKASG
                     HSVEITIPKQGEKKKMLELVKRNSIEFLYKFSEKIDREKEEQEEVDLILSTIFSEKIH
                     RVEAYDISHLSGSFTVGSMVVYEEGKKKPSDYRRFRIRHAKGASDVDSMKEMLSRRLK
                     RLHEEGFGKAPDLILIDGGIQQVRAVEEVVKEMNLSLQICGMVKDDRHRTRALFVDEE
                     TIPLDRSTALYRFIYKIQEEAHRYALNYHHKLRGKGLEASVLTEIPGVGDKRKKALLK
                     HFGSLDKIKKADIEKLCQTPGIDKKTAEAVFDFFR"
     gene            complement(3802..6624)
                     /gene="uvrA"
                     /locus_tag="VFD08_06110"
     CDS             complement(3802..6624)
                     /gene="uvrA"
                     /locus_tag="VFD08_06110"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZK10628.1"
                     /translation="MREEIFVKGAKEHNLKNIDVHIPRNQLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPDVEFIDGLSPAISIDQKSTSRNPRSTVGTI
                     TEIYDYLRLLYARAGTPHCPVCDREISPASLDEVVDRVMELPEKSRVYILAPVVRGAK
                     GSHAKIFDALRKDGFARVKVDGEVISLQETIVLEKNKRHDISVVVDRLVIKEGISARL
                     TDSIETALALEENMVIVEVLGEGEKIFNMNFSCPEHGEGIDEMEPRSFSFNAPFGACE
                     KCKGLGFLQELDPELVIPDLSLSIDKGALLPMKNVKEGSYFGTMIDELLKGTGYTIKT
                     PLEEFSKEAMKRLLYGEKGKLVDVKYKSSRYGKKVVRSKLEGVMQNLQRRYHETNSAY
                     MRAEIEGYMAEIPCSSCGGKRLKPEVLAVKLGGLNIFELTSMSVGDAHDFFENLELPS
                     VKASIAKQIIKEIMARLNFLIDVGLDYLTLARRAGSLSGGESQRIRLATQIGSGLTGV
                     LYILDEPSIGLHQRDNKRLLGTLRNLSDLGNTLIIVEHDEETIESADYILDIGPGAGE
                     HGGYVVAEGSLEDIIKTKESITGQFLSGKRKIHLPKERREPQGEILIKGSKANNLKDI
                     DVKIPLGVFTCITGVSGSGKSSLINEVFYKAMYRRIYRNLSVKPGSFTSIEGWEQINK
                     VISIDQSPIGRTPRSNPATYTKVFDPIRDLFSELPESKKRGYAKGRFSFNVKGGRCEA
                     CKGDGINKIEMHFLPDVYVKCETCKGKRYNRETLEVKYKGKNIYNILELTVSEALEFF
                     ENHSVIRRRLQTLQDVGLGYIKLGQPSTQLSGGEAQRVKLASELSKRSTGQTLYILDE
                     PTTGLHMADVELLIKVLQRLVDGGNSLVVIEHNLDVIKSADYLIDLGPEGGHRGGEVV
                     ATGTPEEVAKVAGSYTGNYLKDIVGV"
     gene            complement(6621..>8032)
                     /locus_tag="VFD08_06115"
     CDS             complement(6621..>8032)
                     /locus_tag="VFD08_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZK10629.1"
                     /translation="YDFKRGTFRGRGDIIEIIPAAKEEHAYRIELFGDEIESISEVQV
                     LTGEWVGKRDHIAIFPASHFVTDKEKIERSLVYIEKELEERIAYFRESGKLLEAQRIE
                     ERTRYDMEMLREMGFCSGIENYSRVLLDRPPGSRPFTLLDYFPDDFLMLIDESHVSLP
                     QVRGMFGGDRSRKQNLVDYGFRLPSALDNRPLNFEEFLTVIGQTIFVSATPGPFEKEH
                     AQHQVDQIIRPTGLLDPLVEVRPSQGQLDNLFAAIRKRTKKNERVLVTTLTKRMAEDL
                     TDYLKEFGVKVTYMHSDIDTIERMKIIRDLRLGEVDVLVGINLLREGLDLPEVSLVAI
                     LDADKEGFLRSETALIQTIGRASRHNEGKVLLYADVVTSSMKKAMDETSRRRKIQIEY
                     NQKHGITPTTIIKDIRDVLAATKKDSVPSSFKEQIKALNKEERLAELELGMFKAANDL
                     DYERAIEYRNAIEELKRKK"
BASE COUNT         2229 a         1880 c         1447 g         2476 t
ORIGIN      
        1 aaacccagta atgccaaggg ttaaggaagc ttttttaggc tttaagctgt caaagtcaag
       61 ctagtaatca taaaggattt ccccacctgt ctcaactttt ttataaaaaa agatgctggt
      121 tttattatca ccaacatcat aaagtgtaca cccattaaaa aaagagccaa ggctcttttt
      181 tttatgccga tatctttttt tctttgtttt ttatctggtc tagggtaaaa attcccagac
      241 caagccaaat aaagacaaaa gaaaaaagcc gcatcaaatc aaaatcttcc ttaaataaaa
      301 atattcccag aagaaatgtg aaactaggac tcaaatactg gataaaagaa atgattttaa
      361 agggaagctt tctagctcca tagccaaata gtagaagagg aagtgctgta accaccccag
      421 tagttgccag caaaagccat acggtccaag gaaagtttcc actaattccc attcctgcta
      481 cttccgaata aagaaaatat ataagagcca ggggggccat aaaaattgtt tctgtataaa
      541 gagagtctat ggcatccaga gggctctgct tttttaaata agtatagatg gcaaaagtaa
      601 gagctagggc caaggccacc cagggcacac gccctagacc cagagtgcta attagcactc
      661 caacaaaagc aaagccattg gctagccact gtttttgatt taatttctct cctaaaagta
      721 ggccaatgag tcccgtaata agaggcaaaa tataatagcc caggcttgct tcaagaacat
      781 ggccgctgtt tatactccaa atataaagaa accagtttag ggtaataaaa actcccccta
      841 aaagactgat ttttcgatgg gacctatcgg ctaaaagttt cttactatag ccccgaccct
      901 ttaaaaaata tatcagtata tgtaatgaaa tcaccgacca cagcatgcgg tgaactaaaa
      961 tttgataggc tggtaaaaaa tccaacacct tccagtatat ggccaataag ccccatataa
     1021 cttgggccag aacggccaaa aatagagctt aattcttttt cattttccac tcctttggga
     1081 tagagataaa ttactagtcc tcctgtgtcc ccctataatc ctacatcaaa aaagacctta
     1141 tgtacattaa aaaagataga aggtctcctt ctatctttgc tctcttaaga ccttttcatg
     1201 gcggaggtcc tctatttttt ttgaaaaagc ctcttcatag caggatggtt ctttactggg
     1261 aatatatagg ccctcaaagg cgtccccagc cacagcaaaa aacaatacaa agacaaagaa
     1321 gatagccaaa attaaaaccg taagctctat ggtatagcta ccactttcct ggttcatagg
     1381 atttcctttg cgataatttc ccatacctcc tctaagagct gctggggtct tttatctgca
     1441 tcaaggcacc taagacgatc ttctttctca cacaaaagaa gatagccctc tcgtatcttt
     1501 ttatgaaaat ccaagttttc atcatccaac cgattcatta ggcgcttatt ttcaatgatg
     1561 cgctccaaac ctttttgggg agcaagatca aggtaaaagg taatgatggg tagatgccca
     1621 tcagtagcaa attcattgag tgcctttatc ttctccatgc caagcccccg ggcatagccc
     1681 tggtaaacaa tagaagagtc tacataacga tccattagaa caattttccc ctctgtcaag
     1741 gcaggaagta ctttttctac cagatgttgc ctacgagatg cggcaaaaag tatggcttct
     1801 gtcattgcat ccataggacg atctgttttt tccaaaagaa tctcacgcac tttttcagca
     1861 acagggactc ctccaggctc tctagtaaga agataaggaa gatttctttc ttctagtttt
     1921 tctactaagc cctttattag cgttgtttta ccactaccct ctccaccttc aaaggatatc
     1981 cactttccct ccatcttctt ctcctatcta aaaaaatcaa aaacggcttc ggctgtcttt
     2041 ttatcaatgc caggggtctg gcaaagtttc tcaatatcag ctttttttat tttgtccagg
     2101 gagccaaaat gcttaagcaa ggcttttttt ctcttatccc ccacaccagg gatctcagta
     2161 agaacagaag cttccagccc cttgccccgg agtttatggt gataatttaa tgcatagcga
     2221 tgcgcctctt cttgaatctt ataaataaaa cggtataggg ccgtagaccg gtctagggga
     2281 attgtctcct catccacaaa aagggccctg gttcggtgac gatcatcttt taccatacca
     2341 cagatctgca gactaagatt catttctttt accacttctt ctactgccct aacctgctga
     2401 attcccccat ctataaggat taaatctggc gctttgccaa aaccctcctc gtgcaggcgc
     2461 tttagtcgtc gacttagcat ttctttcatt gagtccacat cactggcccc cttagcatgg
     2521 cgaatgcgaa atcgccgata gtctgaaggc ttcttttttc cctcttcata gaccaccatg
     2581 gatcccacag taaaactacc agaaagatga gaaatgtcat aggcctctac cctgtggatt
     2641 ttttctgaaa agattgtact taaaatcaaa tctacttctt cttgttcttc tttttctcta
     2701 tcaatttttt ctgaaaactt atataaaaac tcaatggaat tgcgttttac cagttccaac
     2761 atttttttct tctccccttg tttgggaatg gtaatctcaa cgctatgacc agaggccttc
     2821 tttaaatact cttttaccag gccaatctca cttagggtct ctaccacaag ctctcctgga
     2881 ataaaagaag cctcttcata gtattgaaga ataaattctt gcagggtttc accccggtct
     2941 ttttccaata aattatcaaa tatatggttt tccctatcga taattttacc atgacggcga
     3001 acaaaaagtg taatgcagag tctttgttcc ttttcagaaa aactaatata gtctatatca
     3061 tcttttctta gctggctgag ttgctcatga agtgacaatc tttttgccgc atccatctgc
     3121 tctcgaaaaa caatggcccc ttcaaagtcc aaacgctggg ccgcttcatc tctttttttc
     3181 tccaagatcc caataaaggc cttgcggtct tctttaaaaa acccttgaat ttcctctacg
     3241 gccctatcat aatcttcttt cacatggcta aaggcgcaag gggctagaca cttatccata
     3301 tgataatata ggcagggcct ggtaatctta ttaaggggca agtgacactt cctaatagga
     3361 tagatattgt gcagggcttc aaggacatta tttacatcaa aatcacccgt ataaggccca
     3421 aaacaagtgc cccgggaatc taagcccctt accttaaaaa ggcgtggata tttctccttg
     3481 gttaataaaa catagggata gcttttatca tcctttagca gcacattgaa tttgggccga
     3541 tgggccttga taagatttgc ctcaagcagc agggcttctt cttcgctatt ggtaagaata
     3601 tactcaaatt ccttggtatt aactaccagg gcctttgttt ttaccggatg ggagtctact
     3661 cccctaaaat agctagatag cctgtttttt agtttttttg ccttacccac atatataatc
     3721 ttgcccagag catccttcat cgaataaact cctggacttt gaggcacagt atttagttgt
     3781 tctcttatat gagctggaac tttatacacc tacaatatcc tttaaataat tccctgtata
     3841 ggaccccgct actttagcaa cttcttcagg ggttcctgtt gcaacgacct ccccgcccct
     3901 gtggccccct tcgggcccaa ggtcgataag ataatcggcc gattttataa catccagatt
     3961 gtgttcaatc acaaccaggc tatttcctcc atctaccagg cgctgaagca ccttgattag
     4021 aagctctaca tcggccatat gcaggccggt tgtaggttca tctagaatat aaagagtttg
     4081 acctgtgctt ctttttgaaa gttctgaagc aagttttacc ctctgagctt ccccaccaga
     4141 aagctgagta gaaggttggc ccagtttaat atatccaaga cctacatctt gaagggtttg
     4201 caaacgcctg cgaataacag aatggttttc aaaaaactct agggcctcac tcacggtaag
     4261 ttcaagtatg ttatagatgt tttttccctt gtatttgacc tctaaggttt ccctattata
     4321 gcgctttccc ttacaggttt cgcactttac gtatacatca ggaagaaagt gcatttcgat
     4381 tttattaata ccatcaccct tacaggcctc gcaacgtcct cccttgacat taaaggaaaa
     4441 gcgtcctttg gcataaccgc gtttttttga ttctggaagc tcactaaaaa ggtctcttat
     4501 tggatcaaat acctttgtat aggtagcagg attactccta ggtgttcttc ctatgggaga
     4561 ctggtcaata gaaatcactt tatttatttg ttcccagccc tcaatactag taaagctacc
     4621 aggtttaaca gataaattcc gataaatgcg tcgatacatg gccttataaa aaacttcatt
     4681 tataaggcta cttttaccag aaccactcac tcctgtaatg caggtaaaga cccctagagg
     4741 aattttaacg tcgatatcct ttaggttgtt tgccttgctg cctttaataa gaatctcgcc
     4801 ctggggctct cttcgctctt ttggcaaatg gatctttctt tttcccgaaa gaaattgccc
     4861 agtaatcgat tcttttgttt taataatatc ctctaaagac ccttctgcca caacatagcc
     4921 tccatgctct ccagctcctg gtccaatatc aaggatataa tcggcagatt ctatggtttc
     4981 ctcatcatgt tcaacgataa tcagggtatt gccaagatct gatagatttc ttagggtgcc
     5041 aagaagcctt ttattgtccc gctgatgaag gccgatactt ggttcatcta gtatatatag
     5101 gactcctgtt aggccacttc caatttgggt ggccagacgg attctttgac tctctcctcc
     5161 cgagagagag ccagcccttc tggctagggt aagataatct aggcctacat caataagaaa
     5221 attaagtctg gccataattt cttttataat ttgttttgca atactggctt ttacactagg
     5281 cagttccaga ttttcaaaaa agtcatgggc atctcccaca ctcatactag taagttcaaa
     5341 aatatttagg ccgccgagtt ttactgctag cacttctggc tttaggcgtt tacctccaca
     5401 ggaagaacag gggatttctg ccatataacc ttctatctct gcacgcatat aagcggaatt
     5461 tgtttcatgg tagcggcgct ggagattttg cataactcct tcgagttttg agcggaccac
     5521 cttttttcca tagcggctgg atttatattt aacatctact agtttgcctt tttccccata
     5581 caaaagtcgc ttcatggcct ctttactaaa ttcttccaag ggcgtcttta tcgtatagcc
     5641 tgttccctta aggagctcgt caatcatggt cccaaaatag cttccctcct tgacattttt
     5701 cataggaaga agggcccctt tgtcgatact aaggcttaga tcaggaataa caagttcagg
     5761 atcgagttct tgcaaaaaac ccagtccctt acatttttca caggctccaa agggggcatt
     5821 aaaagaaaaa cttcgaggct ccatttcatc tatgccctct ccgtgttcag ggcagctaaa
     5881 attcatattg aagatttttt ccccttctcc aagaacttca acaatgacca tattttcttc
     5941 tagggccagg gccgtttcaa tggagtctgt tagtcttgca ctaatcccct ccttaataac
     6001 cagccggtca accacaacac taatatcatg cctcttgttt ttttctagta ctattgtttc
     6061 ctgcaggctt atcacttccc catccacctt aaccctggca aagccgtctt ttcttagggc
     6121 atcaaagatt ttcgcatggc tgcccttagc gccccgaaca accggcgcta aaatataaac
     6181 cctgcttttt tcgggcagtt ccatcaccct atcaaccacc tcatctaggc tggctggaga
     6241 aatttcccta tcacatactg gacaatgggg agttcctgcc ctggcataaa gaaggcgcag
     6301 atagtcatag atttctgtaa tggtccccac agtggaacgg gggtttctgc tggtcgattt
     6361 ttgatctata cttatggctg gggaaaggcc atcgataaat tctacatcgg gcttttccat
     6421 ttggccaaga aattgtcgtg cataggctga taggctttcc acatagcggc gttgtccttc
     6481 ggcataaatg gtatcaaaag ctagggagct tttgccagaa cccgaaagac cggtaataac
     6541 cactagttga ttacggggaa tatgaacatc tatatttttt aaattgtgct ctttggcacc
     6601 ttttacaaag atctcttctc tcattttttt cgcttaagct cctcaatggc gtttctatat
     6661 tcaatggctc tttcataatc aagatcatta gctgctttaa acataccaag ctctagttca
     6721 gcaagacgtt cttccttatt taatgctttt atttgctcct taaaggaaga agggacactg
     6781 tctttttttg ttgccgctaa gacgtctcta atatctttta taatggtagt tggagttata
     6841 ccatgctttt gattgtattc tatttggatt tttcttctgc gtgatgtttc atccatggct
     6901 tttttcatgc tactggtaac cacatcagca tagagcagga cttttccttc attatggcgg
     6961 gatgccctac caatggtctg aattaaggct gtttcacttc gtaaaaagcc ttccttatcc
     7021 gcatctaaaa ttgctactag ggacacttca ggtagatcta gtccctcacg aagaagattt
     7081 atgcccacta gcacatccac ttcacccagg cgtagatcac gaataatctt catgcgttcg
     7141 atggtatcaa tatctgagtg catataggtg accttgactc caaattcttt cagataatct
     7201 gtaagatctt ccgccatgcg cttggttaga gtagtaacaa gaaccctttc gtttttcttt
     7261 gttctttttc taatagcagc aaaaagatta tccaactgac cctgacttgg tctgacttca
     7321 actaaggggt ctaatagtcc cgtaggacgt ataatttgat ccacttgatg ctgggcatgc
     7381 tctttttcaa aggggcccgg tgtggcagat acaaagatcg tctgcccgat aactgtaaga
     7441 aactcttcaa agttcaaggg tcgattgtct agggctgatg gcaaacgaaa accatagtcc
     7501 actaagtttt gttttcgtga gcggtctcct ccaaacatcc ctcgaacctg gggcagggat
     7561 acatgggact catcgattaa cattaaaaaa tcatccggaa aataatccaa aagagtaaag
     7621 ggcctgcttc ctgggggacg atctagtaaa actctggaat agttttcaat accagaacaa
     7681 aatcccattt ctctaagcat ctccatatca taacgagtac gctcttcaat tctttgggct
     7741 tctagaagct ttccgctttc tctaaaatag gcaatccgct cttccaattc tttttctata
     7801 tataccaggc ttctttctat tttttctttg tcagtaacaa agtgactggc aggaaaaatc
     7861 gcaatatgat ctctttttcc cacccattct cctgtaagga cttgaacctc tgagatggat
     7921 tcgatctcat cgccaaagag ctctatgcgg taggcatgtt cttccttggc agcaggaata
     7981 atctcaatga tatcccctct acctctaaaa gtccctcgtt taaaatcata gt
//
LOCUS       DASRNA010000220         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_144313,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000220 DASRNA010000000
VERSION     DASRNA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144313"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1459
                     /locus_tag="VFD08_06120"
     CDS             89..1459
                     /locus_tag="VFD08_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZK10630.1"
                     /translation="MTIEDKVKIARECFSSGKTKSIDFRLEQLSQLEEGIRKLKPKIL
                     RALKEDLNKSPYEASISEIGIVLGEIHYVKKHLKAWSRPKRVRTALSQAPARSYIYKE
                     PYGLVLIMAPWNYPFQLIINPLIGAISAGNAAVLKPSNYTPRTSEVIEELVQTYLDRQ
                     AFQLVKGGREENQALLDQRFDYIFFTGSPGVGRIVMEAASKHLTPVCLELGGKSPCLV
                     EKTADIKKSAKRILFGKLMNSGQTCISPDYILVDKKVKKALIKELIEQYEKMLPTKEY
                     VDQAFVRIINEKHRLRLEEAVRNQKIIYQDHRISGRHFPFTLVENPDPDSKLMQEEIF
                     GPILPILSYDEIREAFVFIRKREKPLALYLFTKDKKIQRQVLEEVSFGGGCINDTLIH
                     AASPYMPFGGVGQSGMGAYHGKKSFDTFSHKKSIVKKSWYLDLPLRYHPYKKKEEESP
                     EFFFKL"
     gene            complement(1537..2544)
                     /locus_tag="VFD08_06125"
     CDS             complement(1537..2544)
                     /locus_tag="VFD08_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZK10631.1"
                     /translation="MNSIIDLAKRAKEEGLLFRTWGNFSERIDEKSFLITPSGMDYDQ
                     LNKEDLCTMSGAKSTGLHKPSSESPLHERIYKLFSHVKVIMHTHQPYASAIGLSTELH
                     PLDKEMALNFSTAFLPISPYALPSTGKLHKNAIKTLKKHPAKVLLLEKHGAVIMGESY
                     EDVLGRAILLEEVCRKIYYSLFKKEEEIEVNRYSLRKDPIEFYEDNKLSSTPIDAILH
                     DMIYDEREDINVIMVVKSPDLRDFYGSVLYPYLDDFAQIVGIKANESTVHNVLLTLDA
                     ALILAPDMEEALAIKHILDKNAKAARVALYYGAKPIGELESKLMNFIYQEKYAKLKNK
                     K"
     gene            complement(2541..>3853)
                     /locus_tag="VFD08_06130"
     CDS             complement(2541..>3853)
                     /locus_tag="VFD08_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK10632.1"
                     /translation="IEEAKTRIPGGVQHNLAFNHPFPLAFTKAEGAYLYDLDGNKYYD
                     FLQAGGPTVLGSNPEVVRTKVEELLRETGPSTGLFHEYEYKLANLICELMPSVDMFRM
                     LGSGTEACMGAARVARLATKKKHILKMGGAYHGWSDSLAYGIRVPGSKWTQSKGIPKS
                     MFKYTDEFFPGDLEDLEKKLKIKRLTGGVAAVFIEPVGPESGTRPLADDFLQGVRDLC
                     DKYGALLVFDEVVTAFRISPHGAQGYFDVSPDLTIFGKVIAGGYPGAGGIGGKRKYMK
                     YLGAGIQGNEKKALVGGTLAANPLSCVAGYYAIKAIRDEKACEKANAMGDYLTDGLAK
                     LIHKHRLPFVAFNQGSICHLETVGTMHFTIDWSKPWTIPGVIKETSKRKAEMEHMGAA
                     YMAEGIVTLAGSRLYTSAAYTEEMMDDVLERFDRIFGRVVRLGK"
BASE COUNT         1143 a          815 c          821 g         1074 t
ORIGIN      
        1 ctacaatact tatagattga gataagacca agggggggct ggcttgtttc tatttgctgg
       61 taaaaagaaa tagtgagagg aagaaaagat gacaatagaa gataaggtaa aaatagcaag
      121 ggagtgtttt tcttctggca aaacaaaaag tatagacttt cgcttagagc agctcagtca
      181 attagaagaa gggattcgga aactaaagcc aaagatttta cgggccctca aagaagacct
      241 taataaatcc ccctacgagg ccagtattag tgagattgga atcgtactag gggagatcca
      301 ctacgttaaa aaacacttaa aggcttggtc taggcctaaa agggtgcgaa ccgccctatc
      361 ccaagccccc gcaaggagtt atatctataa agaaccctat ggcctagtgc tgattatggc
      421 cccttggaac tatcccttcc agttgataat caatccctta ataggagcca ttagtgcagg
      481 aaatgctgcg gtcttaaaac cgtcaaatta tacgcctcgc acaagcgaag taatagaaga
      541 actagtgcag acctaccttg ataggcaggc ctttcagcta gtaaaaggag gtagagagga
      601 aaatcaagca ctcctagacc agcgctttga ttatattttc tttaccggta gtccaggggt
      661 agggcggatt gttatggaag ctgctagtaa gcacttaacc cctgtttgcc tagaactcgg
      721 aggaaagagc ccatgccttg ttgaaaagac ggctgatatt aaaaaaagcg caaaaaggat
      781 tctttttgga aaacttatga actcaggaca aacctgtatt tcacccgatt atattttagt
      841 ggataaaaaa gtgaaaaagg ctttgattaa agagcttatt gagcaatatg aaaagatgct
      901 gccaacaaag gaatatgtgg accaggcctt tgtaagaatc attaatgaaa aacatcgttt
      961 gcgcttagaa gaagcagtaa gaaatcaaaa aatcatctat caggaccatc gtataagtgg
     1021 taggcatttt ccctttaccc tggtagaaaa tccagatcca gactccaagc taatgcaaga
     1081 ggaaatcttt ggaccgattt tacccatact aagctatgat gagataagag aggcttttgt
     1141 ctttattagg aaaagagaaa aacccctagc cctttaccta tttaccaagg ataaaaaaat
     1201 acagaggcag gtgttagaag aagttagctt tggaggtggc tgcatcaacg ataccctaat
     1261 ccatgctgcc agcccctata tgccctttgg gggtgtgggc caaagtggga tgggtgctta
     1321 tcatggcaaa aaaagttttg acacctttag ccataaaaaa agcattgtga aaaaatcttg
     1381 gtatctggac ctgcccctgc gctatcaccc ttataagaaa aaggaagaag agtcaccaga
     1441 gtttttcttt aagctataag aagtaggaaa tagcagtaaa aaagccagaa gatgttagag
     1501 aatcttctgg cttttctaat gaggaagtag ctcttcttac ttcttatttt ttagttttgc
     1561 gtatttttct tgatagataa agttcataag cttgctttct aactcaccta tgggtttagc
     1621 accatagtag agggcaaccc tagcagcctt ggcattttta tccaaaatat gcttgattgc
     1681 cagagcctcc tccatatcag gagctaagat gagggcagca tccagtgtta gaaggacatt
     1741 gtgaactgtg ctctcattgg cctttatacc gacgatttgt gcaaaatcat caagataggg
     1801 gtagaggaca ctgccataaa agtcccttaa atcaggactc tttaccacca taatcacatt
     1861 gatatcctca cgctcatcat aaatcatatc atgaagaatg gcatctatag gggtggaact
     1921 gagcttatta tcttcataga actcaatagg gtcttttctt agactatatc gattgacctc
     1981 tatttcttct tcttttttaa agagggagta atagatcttc ctacagactt cttctaggag
     2041 gatggcccgc ccaaggacat cttcataact ttctcccata ataaccgccc catgtttttc
     2101 taaaagcaaa accttagcag ggtgtttttt gagggtttta atggcatttt tatgaagttt
     2161 tcccgtagaa ggaagggcat agggactaat gggtaaaaaa gctgtactaa agtttaaagc
     2221 catttcttta tctaaggggt gaagctcagt tgaaagaccg atggccgaag catagggctg
     2281 atgggtatgc ataataacct ttacatgaga aaagagctta tagatcctct catgtaaagg
     2341 actttccgaa gaaggcttat gcaggccagt actttttgcc ccgctcattg tgcaaaggtc
     2401 ctctttgtta agctgatcgt agtccatgcc tgagggggtg ataagaaaac tcttttcatc
     2461 gattcgctca ctaaaattgc cccaggtgcg aaagagaaga ccttcctctt tggcgcgttt
     2521 ggctaaatcg atgatggagt tcatttcccc agcctcacaa cacggccaaa tattctatca
     2581 aagcgctcga gtacatcatc catcatttct tctgtatagg ctgctgatgt atagagccta
     2641 cttccagcca gggttacaat accctcagcc atataggcgg ctcccatatg ttccatctca
     2701 gcctttctct ttgaggtttc tttaataact ccaggaatgg tccagggttt tgaccagtca
     2761 atggtaaagt gcatggttcc cactgtctct aagtggcaga tggaaccttg gttaaaggct
     2821 acaaagggta gtctatgctt atgaattagc ttggctagtc catcggttaa ataatcaccc
     2881 atggcatttg ctttttcaca agccttttca tctctaattg ccttaatggc gtagtagcct
     2941 gctacacaac taagaggatt tgcagcaaga gtaccaccaa ctagggcttt tttctcattg
     3001 ccttggattc cagcgcctaa gtatttcata tattttcttt tgccaccaat tccgccagct
     3061 cctgggtaac caccagcaat caccttacca aagatggtaa ggtctggtga tacatcaaag
     3121 tagccctggg ctccgtgggg gctaatacga aaagcagtta caacttcatc aaagacaagc
     3181 aaggcaccat atttatcgca gagatccctt accccttgta agaagtcatc tgctaggggt
     3241 cttgttccgc tttcgggacc tacaggttca ataaagaccg cggctactcc cccggttaat
     3301 ctttttatct ttagtttttt ctcaagatct tctagatctc ctgggaagaa ttcatcggtg
     3361 tatttaaaca tacttttggg gattccttta gactgggtcc acttgctacc tggtacccta
     3421 ataccatagg ccaaggaatc actccagcca tgataggcac cacccatttt gaggatgtgc
     3481 tttttctttg tggccagtcg agcaactcgt gctgctccca tacaggcttc agtacctgaa
     3541 cccagcatgc ggaacatatc aacagagggc attaattcac aaataagatt tgctagcttg
     3601 tattcatact cgtggaataa accagtcgaa ggtccggttt cccttaatag ttcttctacc
     3661 ttggtgcgaa ctacctcagg attggaccct aaaacggtag ggccgcctgc ttgcaagaag
     3721 tcataatatt tatttccatc aagatcataa agataagcgc cctcagcctt ggtaaaggcc
     3781 aggggaaagg ggtgattaaa agcaagatta tgctgaactc ctccagggat tctagtcttt
     3841 gcctcttcaa tca
//
LOCUS       DASRNA010000221         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_668371,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000221 DASRNA010000000
VERSION     DASRNA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668371"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFD08_06135"
     CDS             <1..216
                     /locus_tag="VFD08_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10633.1"
                     /translation="SKTLDKVSQKMIEELQPYKEFVSEPFGEWLVNERRILIKAEDTT
                     AIKAHLLSLHASKSWQFSVEVDPIESV"
     gene            226..726
                     /gene="def"
                     /locus_tag="VFD08_06140"
     CDS             226..726
                     /gene="def"
                     /locus_tag="VFD08_06140"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZK10634.1"
                     /translation="MAILEIRKDEDPILRKKSKKIKVFDDKLQRLAEDMIETMHEANG
                     VGLAAVQTGRLKQIITLDLYDETGPKVFVNPEIFDPEGEELAYEACLSVTDITAPVPR
                     PISLRLKAQDLEGNPIELEAKDLLARILCHEVDHLKGILFTDKAVDESLMEDQDESLV
                     EDKEEV"
     gene            726..1640
                     /gene="fmt"
                     /locus_tag="VFD08_06145"
     CDS             726..1640
                     /gene="fmt"
                     /locus_tag="VFD08_06145"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZK10635.1"
                     /translation="MNLVFMGTSEFSLGILEHLAKEHNILAIVTRKDRLRGRGKKLLP
                     PPVKVFAMERGIDYFQYLPKAEELKKYPADALIVAAYGKILPKELLELYAYGAINVHT
                     SLLPKYRGASPIQTALINEEKTTGVSIMKMLEGMDTGPVYAQVEVPVGDLRYGELENK
                     LMEKSLPALSKTLKELEKNTALAKAQKEELATYCGKISKSDCLVDWTDSAFKIEGLVR
                     ALWPNPMAYTLRKGERLLLHRVKAFDIDSTKSPGTVEKVTKEGIYVAAGQGLLRIEEL
                     QRPGKRALLAEDYLRGNPITENEILGGR"
     gene            1640..2317
                     /locus_tag="VFD08_06150"
     CDS             1640..2317
                     /locus_tag="VFD08_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017472522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZK10636.1"
                     /translation="MDQLYLLIILIFLLSMWASNNVQRSFIKYSKVPNDRNLSGAEAA
                     RILVQRNNLNVNIERSQGGMLSDHYDPRTNTVRLSPEVFDGRSISSLSVASHEVGHAI
                     QHAYGYKPLEFRTSFFPIASFASKTWTLFFFMGLFFYSGTNFFINIAIGLFAAGLLFQ
                     LLTLPVEFDASRRALVQMTDQGLLGTSEIPGARAVLKSAALTYVMAALMGVANLLRLL
                     SLRGRRS"
BASE COUNT          754 a          418 c          515 g          668 t
ORIGIN      
        1 agcaagaccc ttgataaggt ttctcaaaaa atgattgagg aactgcagcc ttataaggag
       61 tttgtttcag agccctttgg agagtggcta gttaatgaaa gaagaattct tattaaggca
      121 gaagatacta ccgctattaa agctcatcta ctcagccttc acgctagtaa atcatggcag
      181 tttagtgttg aagtagatcc aatagaatca gtataggagt aaatgatggc aatcttagaa
      241 atacgaaaag acgaggaccc catccttaga aaaaaatcaa aaaaaataaa ggtttttgat
      301 gataaattac aaaggctggc agaggatatg attgagacca tgcatgaagc aaatggtgtt
      361 ggtctggccg ctgttcaaac aggtagatta aagcaaataa taactcttga cttgtatgat
      421 gagacaggac ctaaggtttt tgtaaaccct gaaatctttg acccagaagg ggaagaactg
      481 gcctatgaag cctgtcttag tgtgacagat atcacagctc ctgttccaag acccatttct
      541 ttaaggttaa aggcacagga tctggaagga aaccctattg aactagaagc aaaagacctt
      601 ttagcacgaa ttctctgcca tgaggttgat catttaaagg gtattttatt taccgataaa
      661 gcagtagatg aaagtttaat ggaagatcaa gatgaaagct tagtggaaga taaggaagaa
      721 gtataatgaa tttagttttt atggggacat cagaattttc cctaggaatc ttagaacacc
      781 ttgccaagga acacaatata ttggccattg taacgagaaa agaccgcctt agaggtagag
      841 gaaagaagct tttacctcca ccggtaaaag tatttgctat ggaacgtggg attgactatt
      901 ttcagtattt acctaaggca gaagaactta agaaatatcc agctgatgct ttaattgttg
      961 ctgcctatgg gaaaatactt ccaaaagagc ttctagagct ctatgcctat ggtgcaatca
     1021 atgttcatac aagccttctt cccaaatacc gtggtgccag ccccatccaa acggctctaa
     1081 ttaatgaaga aaaaacaaca ggggtcagta taatgaagat gttagagggc atggatactg
     1141 gaccagtcta tgcgcaggtt gaagttcctg tgggagatct tcgctatggt gagctggaaa
     1201 ataagctaat ggaaaaatcc cttcccgcac ttagtaaaac attaaaagaa ttagaaaaaa
     1261 acactgcttt agccaaggca caaaaagaag aacttgccac atattgtggt aaaatttcaa
     1321 agtcagattg cttggtggac tggactgact cagcctttaa aatagaggga ctagtccgcg
     1381 ccctatggcc caatcccatg gcttacactc taagaaaagg tgagcgtttg cttcttcata
     1441 gggtgaaggc ctttgatata gatagtacta aatcaccggg aactgttgaa aaggtgacaa
     1501 aagagggtat atatgtagca gcaggtcagg gactcttgcg tattgaggag ctccagcgcc
     1561 ctggtaaaag agccctactt gcagaagatt atctaagagg taatccgata accgaaaacg
     1621 aaattctagg aggtagataa tggaccaact atacttactt atcatactga tttttctact
     1681 ttccatgtgg gccagtaata atgttcaaag atcctttata aagtattcta aggttccaaa
     1741 tgatcgtaat ttgtctggag ctgaagcagc acgaatcctt gtccaaagaa ataatcttaa
     1801 tgtgaatatt gaacgctcac aaggaggcat gcttagtgat cattatgatc caagaacaaa
     1861 tactgtgcga ctttcaccag aggtttttga tggacgaagt atttcttctt taagcgttgc
     1921 aagccatgag gtagggcatg ccattcaaca tgcctatggc tataaacccc ttgagtttag
     1981 aacaagtttt tttcctatag catcctttgc ttctaaaact tggactttgt tcttttttat
     2041 gggtttgttt ttttatagcg gtacaaattt ctttataaat attgctatag gtctttttgc
     2101 agcgggacta ctctttcaac tcttaactct tcctgtggaa tttgatgcct ctcgtagagc
     2161 cttggttcaa atgactgatc aaggtttatt gggaacaagt gaaattcctg gtgcaagagc
     2221 agtacttaag tcagccgctc ttacctatgt tatggctgca cttatgggcg ttgcaaatct
     2281 attgcgatta ttgtccctgc gaggaagaag aagttgagac aaatatatgc tttggttata
     2341 gaggcacttt tagaa
//
LOCUS       DASRNA010000222         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_966955,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000222 DASRNA010000000
VERSION     DASRNA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966955"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="atpD"
                     /locus_tag="VFD08_06155"
     CDS             complement(<1..1044)
                     /gene="atpD"
                     /locus_tag="VFD08_06155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZK10637.1"
                     /translation="MNKDNQGYIKSIRGSVVEAVFDKNLPAINNKLITGENNEIILEV
                     ASYVDDKTVMAIALTYTGGLSRGNKIIDTGAAIMVPVGLQTLGKMFDVFGNVLNVEED
                     FEAVERKSIHQKPLSFSKRGTSNEIFETGIKAIDVLTPLARGEKAGLFGGAGVGKTVL
                     ITELINNTAQKTKGYSVFCGIGERSREAEELYREVTDTGVLKNAVLIFSQMNEPPGAR
                     FRVGHAALTMAEYFRDVEKKDVLLLIDNIFRFIQAGSEVSGLMGKMPSRVGYQPTLAS
                     ELAELQERIASTPYGAITSIQAVYVPADDFTDPSATHTFSHLSASIVLSRKRASEGLY
                     PAIDPLNSSSNMLN"
     gene            1648..2268
                     /gene="wrbA"
                     /locus_tag="VFD08_06160"
     CDS             1648..2268
                     /gene="wrbA"
                     /locus_tag="VFD08_06160"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HZK10638.1"
                     /translation="MSKTKLAIVYYSMTGTNFQLASWAKEAAEEIGAEVRMLKVHELA
                     PQEAIESNPAWKAQVEKEKDIKEASSEDLLWADAIIFSSPTRFGNVASQVKQFMDLQG
                     GVWAQGKLADKVVSAMSSAQNPHGGQEQTIQSIYTTMMHWGAIIVAPGYTDGSVFLAG
                     GNPYGTSVTAGSDGMVEDVEASVKHQARRTVEVARLLKLGRASEDK"
BASE COUNT          691 a          435 c          476 g          749 t
ORIGIN      
        1 atttagcata ttggatgagg agtttaaagg atcaatggct ggatacaaac cttcacttgc
       61 tctttttctg gataatacaa ttgaggcaga caaatgagaa aatgtatggg tggcagaagg
      121 gtctgtaaag tcatctgctg gtacgtacac tgcctgaatt gaggtaatgg ccccataggg
      181 tgtgctggcg attctctctt gtaattcagc cagttcgctc gccagggttg gttgatagcc
      241 tacccgagaa ggcattttac ccatgaggcc tgaaacttca gaccctgctt ggataaatct
      301 gaaaatatta tcaatgagta agagtacatc ttttttctct acatcacgaa agtactctgc
      361 catggtaagg gcagcgtggc caactctaaa tcttgctccg ggtggctcat tcatttgaga
      421 aaatataaga acggcatttt taaggacacc tgtgtctgtt acttcacggt agagttcttc
      481 tgcttctctt gagcgttctc caataccaca aaagacgctg tagccctttg ttttttgcgc
      541 tgtattattt ataagctctg taattaaaac tgtctttcca acaccagctc ccccaaatag
      601 gcccgctttt tcaccacgag ctagtggagt tagtacatca atggccttaa tgcctgtttc
      661 aaagatttcg tttgatgtgc ctcttttaga aaaagataag ggcttttgat ggatggattt
      721 tctttctacc gcttcaaaat cttcttctac attgagtaca tttccaaaga cgtcaaacat
      781 ctttcctagg gtttgaaggc ctactggcac cattatggca gctcccgtgt cgattatttt
      841 atttccccta gaaagacctc ccgtataagt tagggctatg gccataactg ttttatcatc
      901 tacataggaa gccacttcta gtataatttc attattctca cccgttatta gcttgttatt
      961 aatagctgga aggtttttat caaaaacagc ctccacaaca ctgcctctaa tggacttgat
     1021 ataaccttga ttgtctttat tcataaggat tctccctatt tcacttaact tttttattga
     1081 cctgtttttt ataatttcaa atgcttgatt ttggattctc catagttagg tagttcattt
     1141 ataagctgct ttttttacca taaatacata aataatcagt ttaatgcttc ctataaaatc
     1201 cagcatatgt atttattata acacatgcat tttgattatt tagttaggtc ttgatcaaaa
     1261 ccgtgtcaag tttgcaaaag ctgctattgt tctgtcttga tgtatagctt gtgattttaa
     1321 tctgataaat caactaaaga aaaaggagct tggtgtttct ttatttcttc ttgatcttgg
     1381 ggcaagcaat taagtgccct gcatcagggt cacttaagtc gcatcacccc tatgcatttg
     1441 gcttaaagca taatttcctg aaaaagtaaa tttaggcaat ttaagtaaga ggaaattata
     1501 gggtggatta tcctttttat aacgggtcta gtcttctttc tacgaaaggc tctctttttt
     1561 tgtgctatat agtaagtttt ttgggtatat gttaatcagc taacacaata aagatatatt
     1621 caattatatt ttaataagga ggaaagaatg agtaaaacaa aactagctat agtctactat
     1681 agtatgacag gcacaaactt tcagctggct tcttgggcga aagaggcggc agaagaaata
     1741 ggggcagaag taagaatgct taaggtgcat gaacttgcgc cacaagaagc aatagaaagt
     1801 aatcccgcct ggaaagccca ggtagaaaaa gaaaaagata ttaaagaagc tagctcagaa
     1861 gatcttttat gggcagatgc aataattttt tcttctccaa cgcgttttgg aaatgttgcc
     1921 tctcaagtaa aacagtttat ggatttacaa ggtggtgtat gggctcaagg aaaacttgcc
     1981 gacaaggtcg tttctgctat gagttcagct caaaatccac acggagggca agaacaaacc
     2041 atccaatcaa tctatactac aatgatgcac tggggagcga ttatagtggc tccaggctat
     2101 acagatggta gtgttttctt agcgggtgga aacccttatg gtacttctgt tacagccgga
     2161 agtgatggaa tggtagaaga tgtagaagcc tccgttaaac atcaggctag aagaaccgtt
     2221 gaagttgcaa ggcttctaaa gcttggaaga gctagtgagg acaaataaat aggcagtatt
     2281 tagctaaagt aaaggtagcc cttgataaaa agagctgcct tttttattgt catcattcaa
     2341 atatatgccg g
//
LOCUS       DASRNA010000223         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_881637,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000223 DASRNA010000000
VERSION     DASRNA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881637"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1213
                     /locus_tag="VFD08_06165"
     CDS             104..1213
                     /locus_tag="VFD08_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HZK10639.1"
                     /translation="MDKTRTVDWKTVTNFIVDALEGYGVPREDAKICADVLTDADKKG
                     IESHGINRLKPIYIDRIKAGIQEPLTKIDIIKETASTAVLDANNGMGMVASYKAMEMA
                     MDKARKNGIAMVALRNSTHYGAAGYWTDMAAKKGLVAMTGTNARPSIAPTFGVENKLG
                     TNPLTVSAPTGEDFPFMLDCATSIIQRGKIEYWNRLGQDVPSGMVVGKDGQPQTDSAK
                     ILQDLVKGEAALAPLGGIGEEFGGHKGYGYATIVELFSAAFSGADYLGALSGVTEDGH
                     KKPILLGHWFIVIDPEAFMGLDIFEKIAGGIMKELRESKKAPGKERIYTAGEKEHLVW
                     LERKEKGLPINEALQKEIIGIRDELKLDYSFSFEK"
     gene            1486..2463
                     /locus_tag="VFD08_06170"
     CDS             1486..2463
                     /locus_tag="VFD08_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HZK10640.1"
                     /translation="MNKIRIEDSAKVIGKVRLGKNAYLAQGTIVSSYNDSVSIGNSSW
                     LLENSVIIGTEENPVKVGSKTVFGHKSVTIGAEIGDLCEIGNNTIFLPGSKVGDMCVF
                     GEGTIVPENMVIPSQSVVLGRPAKVIRKLNENDKNMIKKMRGSDISLDEFVEHIFEFE
                     RGESAMTNLYEYKGKLPEVAASSYIDNLAEITGDVVIGENSYIASGVRIIGDSHGPIK
                     IGNNVTILENSVLHLLPDNELIIHDNVTIGPNSTIHGTVIGSQSIVEAGSIVCDYSVI
                     GENSLIKAGTLVKQRDKFEKNSILEGFPAKEIGKTRERLKRPAWAKRIV"
     gene            2587..>3050
                     /locus_tag="VFD08_06175"
     CDS             2587..>3050
                     /locus_tag="VFD08_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK10641.1"
                     /translation="MKENKDRLAYPAAVAYAVIIGLSFLFTKLALDVASPIDILAHRF
                     TVSFFAIAIPLLFKWVKVDYNRQRIIKIIPLAIIYPMLFFGFQTFGLQHATSSEAGIL
                     LASAPVFTLILASYFLKEKSTSLQKLSVVLSVTGVIYISVMKGSTLDVNNIKG"
BASE COUNT         1028 a          535 c          693 g          794 t
ORIGIN      
        1 gttatacttt aaacaggatt aggataggtt cagcgaggtg ttttgtagtg cttactacgg
       61 caatacttcg tttttttatt cgtattttga aaggagaata tcaatggata aaacaagaac
      121 tgttgactgg aaaacagtaa ctaactttat cgtagatgcg ctagagggct atggggtccc
      181 cagggaagat gcaaaaattt gtgcagatgt cttgactgat gcggataaaa aaggcatcga
      241 atctcatgga ataaatcgac tcaagccgat ttatattgat cgaatcaagg caggaatcca
      301 agagcccctc accaaaattg atattattaa agaaacagcc agcacagcag tcttggatgc
      361 aaataatggc atgggcatgg tggcatctta taaggctatg gaaatggcta tggacaaggc
      421 aaggaaaaat ggaattgcta tggtggccct aagaaattcc acccactatg gtgctgcagg
      481 atactggaca gatatggctg cgaaaaaagg actggtggct atgacaggca ccaatgcccg
      541 cccctctatt gccccaacct ttggtgtaga gaataaacta gggacaaatc ccctaacagt
      601 cagtgcacct acaggggagg attttccctt tatgctagac tgtgccacat ctattatcca
      661 aagaggaaaa atcgaatact ggaaccgact tggacaagat gttccttcgg ggatggtagt
      721 aggaaaagat ggccagcctc aaaccgattc ggctaagatt ttacaggatc tggttaaggg
      781 agaggcagcc ttagctcctt tagggggaat aggagaagaa tttggtggcc ataaaggcta
      841 tggctacgct actatagttg agctgttttc cgccgccttt tccggagcag attatcttgg
      901 agccttatca ggagtaacag aagatggtca taaaaaaccc atcctcctag gtcactggtt
      961 tattgttatt gatcccgagg cttttatggg actggatatt tttgaaaaaa tagctggtgg
     1021 cattatgaaa gagctaaggg agtccaaaaa agcccccggg aaagaaagaa tttataccgc
     1081 gggagaaaaa gagcacctgg tctggctaga gcgcaaagaa aaaggccttc ccatcaatga
     1141 agccctccaa aaagaaataa taggaattag ggatgaactc aaactagatt actctttttc
     1201 ctttgaaaaa taagttgatt tttagtaagt tgccttataa atatacccta gaaggataaa
     1261 agaagaactt ccataaataa gatagcccct ttacttgagc ttttaaggcc cttaagtaga
     1321 ggggctactt atttaaaggg gcaattgata gaaagaagta gagatatttg atacaattag
     1381 aaaaagggag tatgcttttt cctgtttttc cataaaaaaa gaataacttg ctatagaaat
     1441 taaggcaagt ttagtgaaca agaagggtaa tttttaggag gcaaaatgaa caagataagg
     1501 atagaagatt ccgcaaaggt tattgggaaa gtgagactag gaaaaaacgc ctatcttgcc
     1561 cagggcacca ttgtaagctc ctataatgac tccgtaagca taggaaattc atcttggcta
     1621 ctggaaaact cagtcattat aggcacagag gaaaacccag tgaaagtagg cagtaaaaca
     1681 gttttcggtc ataaaagcgt taccattgga gcagaaattg gagatctatg cgaaattgga
     1741 aataacacca tctttttacc aggttcaaaa gttggggata tgtgtgtttt cggtgaaggg
     1801 accatagtcc ctgaaaatat ggtcattcca tcccagtcag tggttctagg aaggccagct
     1861 aaggtaatta gaaaactaaa tgaaaatgat aaaaatatga ttaaaaagat gagaggcagt
     1921 gatatctcac ttgatgagtt tgtagagcat atatttgaat ttgagagagg agaaagcgcc
     1981 atgaccaatc tatatgaata taaggggaaa ttaccagagg tagccgcttc tagctatatt
     2041 gataatctag cagagattac aggagatgtt gtgatagggg agaattccta tatcgcttcg
     2101 ggcgttcgga ttattggtga ttcccatggc ccaataaaaa taggaaacaa tgtaaccata
     2161 ttagaaaatt ctgttctgca cctattgccg gataatgaac taattatcca tgataatgtg
     2221 accatcggcc ctaattcaac catccatgga acagttattg gtagccaaag catagttgag
     2281 gcaggctcca tagtgtgtga ttatagtgta ataggagaaa acagcctaat taaagcaggc
     2341 accctagtaa aacaaagaga taaatttgaa aaaaactcta ttctagaagg gtttcccgca
     2401 aaagaaatag gaaaaactag ggagcggctt aaaagaccgg cttgggcaaa aagaatagta
     2461 taaattctta gaggaaagct cccatctaag agaagataat agactctgct accttagcca
     2521 ctggcaagaa ctagcagaag tgataaggaa atagaggtta actgggagta ggaaaaggaa
     2581 ggaaagatga aagaaaataa agacagatta gcgtatccag ccgcagttgc ctatgcagta
     2641 attataggcc tgtccttttt atttacaaaa ctcgccctgg atgtggccag tcccatagat
     2701 atactggctc acagatttac cgtatcattt tttgctattg ctatccccct cctgtttaag
     2761 tgggtaaagg ttgattataa taggcaaaga attataaaga ttattcccct agcaattatt
     2821 tatcccatgt tgttttttgg atttcagacc tttggactcc aacacgccac atcttcagaa
     2881 gcaggtatcc tcttggcatc agccccagtc tttaccttga ttctggcaag ctacttctta
     2941 aaagaaaagt ccactagctt gcaaaaacta tctgttgtcc tatcggtaac aggagtgatt
     3001 tatatctcag taatgaaagg gtccacccta gatgtaaata acataaaggg
//
LOCUS       DASRNA010000224         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_145916,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000224 DASRNA010000000
VERSION     DASRNA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145916"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFD08_06180"
     CDS             complement(<1..748)
                     /locus_tag="VFD08_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZK10642.1"
                     /translation="MSTLLYVGILLLSGLFFGKLAHRIGFPNVTGYLIAGLIIGPHAL
                     GIVPIEAVDGIGIVSELALGFIALSIGSELEWSYFKKLGMTSVVIAVFESLFAMGFVI
                     IALLIFGFELPLAFMLGAIASATAPAATIMVIRQYRAKGPLTRTLLSVVAIDDAIAII
                     AFGFAATFAKTISSGIPFSIMTLFSPFIELALSIIIGVVFALVLLIPLHYFKKESNRM
                     CGILGVISITSAVATYTGASALLACMVLGIV"
     gene            complement(745..1101)
                     /locus_tag="VFD08_06185"
     CDS             complement(745..1101)
                     /locus_tag="VFD08_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10643.1"
                     /translation="MKLFVLILNHIEKLDDLMVAFAKENIKGATIFDSTGMARELSDS
                     GYREEEIPFLVSLRGYLNYDDGRKNKTILTVIRDDQQETIIKTTKEVVGDFSEPDTGI
                     MFILPLDYVVGRGQEK"
     gene            complement(1494..2198)
                     /locus_tag="VFD08_06190"
     CDS             complement(1494..2198)
                     /locus_tag="VFD08_06190"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZK10644.1"
                     /translation="MVERIDKIIASQGKYSRSQVKKLIRQNKVEVDGEIIKSSAIKVD
                     PDKSRITLEGLDIIYREFIYLILNKPKGYVSARVDKDHQTVLDLVPKELTGRKLFPAG
                     RLDIDTRGLMIITDDGLFAHNILSPKKDIKKTYQVELDIPVTEEMVQGFASGVELNDG
                     ICKEALLEIRSKKTARVTLKEGRYHQVKRMFGCFGAKVIELQRVAMGNLTLPEDLPEG
                     LSRELSDQELELLQQL"
BASE COUNT          740 a          522 c          394 g          772 t
ORIGIN      
        1 atacaatacc aagcaccata caggccagta gggcagaagc acccgtataa gttgcaactg
       61 cagaggttat tgatataaca ccaaggatcc cgcacattcg attggattcc ttcttaaaat
      121 agtgcagtgg tataagtaag accaaggcaa aaacaacccc aattataatt gataatgcca
      181 gctcaataaa gggagaaaaa agggtcatta tgctaaaggg gataccagag ctgatggttt
      241 ttgcaaaggt ggcagcaaat ccaaaggcga ttatagctat agcatcatca attgcaacaa
      301 cgcttaacaa ggttctggta agaggcccct tggccctata ttgtctaata accataattg
      361 ttgcagctgg tgctgtagcc gatgcaatag ctcccagcat gaaagcaagg ggaagctcaa
      421 atccaaagat aagtaaagca attattacaa atcccatagc aaataggctt tcaaagactg
      481 caatcacaac agaagtcata ccaagctttt taaagtatga ccactctagt tctgatccta
      541 ttgatagcgc aataaaaccc agtgctaatt cggatacaat accaatccca tcaactgctt
      601 caatggggac aatcccaaga gcgtgggggc ctataattaa ccccgcaata agatagcccg
      661 ttacattggg aaaaccaatc cgatgggcaa gttttccaaa gaacagtccc gataataaga
      721 gtattccgac atataataag gtactcactt ttcttgacct cttcctacta cataatctag
      781 cggaagaata aacataattc ctgtatcagg ctctgaaaaa tccccaacca cctcttttgt
      841 tgtcttgata atcgtttcct gttgatcatc gcgaatgacc gttaaaatgg tcttgttttt
      901 tcttccatca tcataattta aatagcctcg aagtgatact aaaaagggta tctcctcttc
      961 cctataaccc gaatcagaaa gttcccgcgc cataccggta ctgtcaaaaa tcgttgctcc
     1021 cttaatgttt tcttttgcaa aggcaaccat taaatcatca agtttttcaa tatgattcaa
     1081 aatcaaaaca aataatttca ttcttatccc atcctatctg agttttcgta attctagttg
     1141 tagtctttct tatagcatct gtattagatg ttagcgacct ctttttttgc tactactttc
     1201 ttaaatttta gtacatattt gataaaaaag cgaaatgccc atttgagttc aagcactcac
     1261 cttctctatt atacaccaca ggagtaaagc atttgatctc tatgtctttt aaagcactta
     1321 ctacctgtgg ctaaggaaaa taaagagctc ttttctaatt ctttttttct atccacttaa
     1381 aagggaaagc acccaaatta ctaggtgctc tttctctttt atggtttttc tatcttagaa
     1441 ccactagaag gctctttgtt tttcctggga gctatcgtct ttattgaagg tttttataat
     1501 tgttgaagaa gctctagctc ctgatcacta agctctctag ataaaccttc tggaaggtct
     1561 tcaggaaggg tgagattgcc catggcaact cgctgaagtt caatgacctt ggcaccaaag
     1621 cagccaaaca tacgcttaac ttgatgatag cggccttctt tgagggttac cctagcagtc
     1681 ttcttacttc ttatttccaa tagggcctct ttacagatcc catcatttag ctcaacacca
     1741 ctggcaaagc cttgcaccat ttcttcagta acagggatat ctaattctac ctggtaggtt
     1801 ttttttatat cttttttagg tgaaagaata ttatgagcaa aaagaccatc atcagttata
     1861 atcattaagc ccctggtatc tatatccagt cttcctgcag ggaataattt gcgaccagtc
     1921 aattctttag ggactaaatc caaaacagtc tggtggtctt tatctactct agcggacaca
     1981 tagcccttgg gcttattcaa gataagataa ataaactctc gatatataat atcaaggccc
     2041 tcaagagtta ttctactttt atcaggatct acctttatcg cagaagattt gatgatctca
     2101 ccatcaactt ctaccttgtt ttgtcgtatt aattttttca cttggcttct tgaatacttt
     2161 ccttgcgaag caattatttt atctatacgt tccaccatta aacccctttt cttattgtgc
     2221 ctcttaagtt taacatatat aattatcggc tacaaatact ctagatagcc tatcaaataa
     2281 tactttttcc tacttctttt ttccacttgt tttgccttta aagcaaaggt gttatcgtca
     2341 ttttttgcaa aaatgcctag ccttatgtaa caaatcacaa aggcatgtga attggtaatt
     2401 tctttactat tgctatacaa tataagat
//
LOCUS       DASRNA010000225         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_593040,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000225 DASRNA010000000
VERSION     DASRNA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593040"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..872)
                     /locus_tag="VFD08_06195"
     CDS             complement(246..872)
                     /locus_tag="VFD08_06195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZK10645.1"
                     /translation="MDAIIFDLDGTLWNATKPAFKALRHIEDKYGAQATDYDTFCSLM
                     GKQMDEIAQKTMKGLAQEKKMELFQEFYTLELALIRQEGSLLYPDMVSTLKKLKDLYP
                     LFIVSNCQEGYIETFLQTHGLEDLFVDHLCWGERKRPKGENLLYIMKKHGLKNPIYVG
                     DTRGDEIAADFAGINFYYVDFGFGQAKKPFRRISSYKQLEQELLTVMK"
     gene            complement(872..2173)
                     /locus_tag="VFD08_06200"
     CDS             complement(872..2173)
                     /locus_tag="VFD08_06200"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HZK10646.1"
                     /translation="MSQSNFIIELLKNEVRPAIGCTEPVAIALGTAYGAQLIGEKPTK
                     ITVLTNKAIYKNGMDVGIPGTEKTGLDVAGALGAALSDPSKGLELLGEVTPEKKEIAD
                     SYLKEIPIRVDIADTDKAVYIKVLIESKNHKAEVLIYDRHDQLIELSLDGKVLSTIHK
                     DAVTEGLSDNNDEKEKFYRFKLEEIVETIEKIPAEKLHFLLEGLEMNKKAAQLGMEKR
                     FGLGVGFNMYQNMKEGLISEDFANLAFVLTAAASDVRMSGERVSVMSSSGSGNNGLTA
                     ILPLVALQKVKSLDDATLARALAISHTITSYVKYYIGRLSNLCGCSIAAAIGSGSAIA
                     WVLGGKSAIAGTVNNIIANQAGVVCDGAKPGCSLKLGTAAMTAVQAALLSLKGTIIID
                     HNGLTDPCAEKSIENLALLSSKGMKDVDESIIDIMQNRRSY"
BASE COUNT          713 a          527 c          477 g          803 t
ORIGIN      
        1 agtcttggat ggtggggagg tggaaccatc tacggtttag ctatgttgac agagtttaat
       61 ctctgattca cgctactaga gagtagggtt cacggcgaaa ccaattgcct gttggcaggg
      121 gtaaccctga tccacgtata acagaatggc agaggccgaa agccactttt ctaagactcc
      181 gaacctaaac caataataca attacatgac ggttttatag ttgatccttt tctgcttgac
      241 aagggtcact tcataacagt taaaagctct tgttctagtt gcttataact agaaatcctt
      301 ctaaaaggct tttttgcctg accaaagcca aaatcaacat aataaaaatt tattccagca
      361 aaatcagcag ctatttcatc ccccctagta tcaccgacat atatagggtt ttttagtcca
      421 tgctttttca taatataaag aagattttcc cccttgggtc tttttctttc tccccagcaa
      481 aggtgatcaa caaaaagatc ttccaagcca tgggtttgta gaaaggtttc aatataaccc
      541 tcttggcaat tactaacgat aaaaagtgga tataggtcct taagtttttt tagggtgctt
      601 accatatcag gataaagtag actgccttct tgcctaataa gagccagctc aagggtataa
      661 aactcttgaa aaagctccat tttcttttct tgcgccaggc ccttcatggt cttttgagca
      721 atttcatcca tttgcttacc cataagggag caaaaagtgt cgtagtcagt ggcttgagca
      781 ccatatttat cctcaatatg gcgtagggct ttaaaggctg gcttagtggc gttccagaga
      841 gtaccatcga gatcgaaaat gattgcgtcc attaataact ccttctattt tgcataatgt
      901 cgataatact ttcatcgaca tccttcattc cttttgaact cagtagggct agattttcaa
      961 tgcttttttc tgcacagggg tcagttagtc cgttatgatc aataataata gttcctttta
     1021 gtgaaagtag ggctgcttgt acggcggtca tggcagctgt accaagcttt aaggaacatc
     1081 ctggttttgc cccatcgcat acaacaccag cctgattggc aataatatta ttgactgtac
     1141 cggcgatagc actttttccc ccaaggaccc aagcgatggc agaaccgctt ccaatagcag
     1201 cagcaatact gcaaccgcaa agattagaaa gtctaccaat ataatattta acataactgg
     1261 ttatagtgtg actaatagcc aaggccctag ctagggtagc atcatcaagg gattttactt
     1321 tttgaagagc gacaaggggt aaaatagcag taagcccatt gtttccgctt cctgaagagc
     1381 tcattacact gactctttct ccgctcatgc gcacatcgct agctgctgct gttaagacaa
     1441 aggctagatt ggcaaaatct tctgaaataa gcccttcttt catattttgg tacatattaa
     1501 agccaacacc taggccgaaa cgtttttcca tccctaactg ggcagctttt ttattcattt
     1561 caagtccctc aagaagaaag tggagttttt ccgcggggat tttttcaatg gtttcaacga
     1621 tttcttctag cttaaaacga taaaactttt ctttttcatc attgttatct gaaagtccct
     1681 cagtcacagc atctttatga atggtagata aaacttttcc atccaggcta agttcgataa
     1741 gctgatcatg gcgatcatat attaagacct ctgccttgtg gtttttgctt tcaataagaa
     1801 ccttaatata aacagccttg tctgtgtcag ctatatcaac cctaataggg atttctttta
     1861 ggtagctatc agcgatttct tttttttctg gagttacctc accaagaagc tctagaccct
     1921 tagagggatc actaagggct gcccctaagg cgccagctac atcaagccct gttttttcag
     1981 tgccaggaat acctacatcc atgccgtttt tatatatagc cttattggtt aaaacagtta
     2041 tttttgtagg tttttcacct ataagttgag ccccataagc tgtacccaag gcaatagcca
     2101 cgggttctgt acaaccgata gcagggcgaa cttcattttt taaaagctcg ataataaaat
     2161 ttgattgaga catttttttc tccatttttt cttttgtcgg cttgtataga gtaacaaagt
     2221 ctattctaca ccattttgta aaaagaataa aacttttaaa atgaccagag agttggacac
     2281 agtgtttttc ttgggttata cttaaaaaca ccatggtaaa atttaaaaca cgaagtgaat
     2341 ttataataaa agaagatctt taccgggtaa tatggaccct gagccttcct ttgatgctga
     2401 acaatttaat acaaactcta tataattttg tagatgcctt ctggcttgga cgtttgggtc
     2461 acgagcagtt tgccgcgact agttttgttt ggccagttgt gcacttgttt gtttctatcg
//
LOCUS       DASRNA010000226         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_593041,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000226 DASRNA010000000
VERSION     DASRNA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593041"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..847
                     /locus_tag="VFD08_06205"
     CDS             164..847
                     /locus_tag="VFD08_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10647.1"
                     /translation="MKRNRILLVIILLCVVLTACSGSEGLAPLPAPQVDQSSKFGVDQ
                     NINMETIDNYLHREDVAYRDVRMLFDPADYAAVGGDADLSQTIHGFKIVPYPYLATLS
                     ELPVTGAYNDRCLFNVEWTDSREILSAEANYKESMQILEELFPKDQAIFIMCGGAGYS
                     QMMKELLIHLGWDEALLYNTGANWSYTGDNSLELIIYSEDAKEPNTYAIWRADYAYLL
                     FDKLQPIKD"
     gene            859..1443
                     /locus_tag="VFD08_06210"
     CDS             859..1443
                     /locus_tag="VFD08_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10648.1"
                     /translation="MIKKSRFTLLLLMLILFTACSQPTDKALVQESYPYPTFDFTHFA
                     SGGNAEIYPAVILFEQSVSTFTSYQVAFVSCTCRDSLVNYYSVCYVELLNNKPSAEQS
                     AIRSITFGQNQGLWGDSNPNYYISEYTQEYMDEHFVQNLVKMTKEEIDAWEGYGSSLE
                     TVDIDAISGATVSTGNITSMLQGLFAYHAEKYYE"
     gene            complement(1547..2080)
                     /locus_tag="VFD08_06215"
     CDS             complement(1547..2080)
                     /locus_tag="VFD08_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HZK10649.1"
                     /translation="MQEKRHIFLTGDKQVGKSTLWKKVLEELDISPWGFLTLPYTVKG
                     NFRGFCLHGLGPLPPGFENDIPISIYLQARKHRPIVDSFEEFGSRLLERAQEDLNYIM
                     MDELGRFEKNAPNFQKAVMQCLDGDHHVLGVLQDTQNPFIDKVRERQDILLITVDKKN
                     REDLVEEVIAALKKLKS"
BASE COUNT          628 a          492 c          476 g          728 t
ORIGIN      
        1 ggtaaccctg atccacgtat aacagaatgg cagaggccga aagccacttt tctaagactc
       61 cgaacctaaa ccaataatac aattacatga cggttttata gttgatcctt ttctgcttga
      121 caagggtcac ttcataacag ctaacatatc agaaagggat tttatgaaaa gaaatagaat
      181 tcttcttgtc attatactcc tttgtgttgt tcttactgcc tgcagcgggt ccgagggcct
      241 tgcccctctg cccgccccac aggtagatca gagcagcaag tttggagtag atcaaaacat
      301 caatatggaa accattgata attacttaca tagggaggat gttgcctata gggatgtgcg
      361 tatgctcttt gaccccgcag attacgcggc tgttggtggc gatgcggatc tatcacaaac
      421 catccacggc tttaagattg tcccctatcc ctatctagcc accttatcag agctacctgt
      481 tacaggagcc tataatgatc gttgcctttt taatgtggaa tggaccgata gcagggagat
      541 tctttctgcg gaagcaaatt ataaggaatc catgcaaatt ttagaagaat tattccccaa
      601 agatcaggcc atttttatta tgtgcggcgg cgccggatat tctcaaatga tgaaagaact
      661 actcattcat cttggatggg atgaggccct actctataat acgggagcaa actggtccta
      721 tacaggagac aatagcctgg aattaattat ttattccgag gatgccaagg agccaaatac
      781 ctatgccatt tggcgagcag attatgctta tttactcttt gacaaattgc agcccattaa
      841 agattgagta taaaaaagat gataaaaaaa tcaagattta ccttacttct attaatgctt
      901 attctcttta ctgcttgctc ccagccgact gataaggcct tagtccaaga aagctacccc
      961 tatccaacct ttgattttac ccactttgct tcgggaggaa atgccgaaat atacccagcg
     1021 gtaatcttat ttgagcaaag tgtctctacc tttacttcct atcaggtggc ctttgtgtcc
     1081 tgtacttgcc gggatagtct ggtaaactat tactctgttt gctatgtaga gctccttaat
     1141 aataagccca gtgctgagca atcagccata cgctccatta cctttgggca aaaccaagga
     1201 ctttggggag atagtaatcc caattattat atatctgagt atacccagga atatatggat
     1261 gagcattttg tccaaaacct agtcaagatg accaaggaag agatcgatgc ctgggaggga
     1321 tatggaagta gcctagaaac ggtggatatc gatgctattt ctggtgcaac ggtttctacg
     1381 ggaaatatca cttctatgct acagggactt tttgcttatc atgcagaaaa gtattatgag
     1441 taaaaagggt cctaatacat cttcttgcta cttttatagg gatatttagg cacaaaaaag
     1501 ggaggctcca gaactttttc tagttctagg gccttccttt tatttatcat gactttagtt
     1561 ttttaagagc agcaattact tcctctacta ggtcctcccg gtttttttta tcaactgtaa
     1621 taagtaagat gtcttggcgc tctcggacct tgtcgataaa ggggttttgg gtgtcttgga
     1681 gtacccctaa aacgtgatgg tctccatcta ggcactgcat tactgccttt tgaaagtttg
     1741 gagcgttttt ttcaaacctt cctaattcat ccatcataat atagtttaag tcctcttggg
     1801 ccctttctag tagccggctg ccaaattcct caaagctatc tactatgggt ctgtgttttc
     1861 tggcctgaag atagatggat attgggatgt cattttcaaa gcctgggggc aaggggccca
     1921 ggccatgaag acagaagcca cggaaattgc ccttgactgt atagggtagg gttaaaaaac
     1981 cccaagggct tatatccagc tcttctagta cttttttcca tagggttgac tttcccactt
     2041 gtttgtctcc tgttaaaaaa atatggcgtt tttcctgcat ctttcacctc agatctgctt
     2101 tcctttattt ttctatattt gtcctctatg attttatacc tgttatgatt gattttatcc
     2161 cctaggggca cttgtgtttt atgctcttaa gaaaagagtt gattctttgc gaatcatatt
     2221 cttcaaaata ggaagcctct gcccagatcc ttagctcctt gtcttctgga ggagcggtca
     2281 ctactccatc taaaaggtgg gatatccaaa atagtagtct tcta
//
LOCUS       DASRNA010000227         7916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_1040555,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000227 DASRNA010000000
VERSION     DASRNA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040555"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFD08_06220"
     CDS             <1..654
                     /locus_tag="VFD08_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZK10650.1"
                     /translation="ILTKSISRFGRDTVDTLEALNQLKTLGVRVIFEQEDLDTSNTDS
                     DLMISIIEAIAQSENESRSENIKWGIKQRAAQGTSKLYNRKCYGYVHDKEGNLIIKDD
                     EAKNVTKIFEMYLHGKSIIGIVAELERLGVKSPTGKDKWSKRTIDVMLSNEKYTGSVR
                     LLDNGKHEVVYLCEDNNPAIISKETFQAVQIEKTKRSNVIKGENGVQRKSKKYSSKQ"
     gene            772..1155
                     /locus_tag="VFD08_06225"
                     /pseudo
     CDS             772..1155
                     /locus_tag="VFD08_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
     gene            complement(1385..1600)
                     /locus_tag="VFD08_06230"
     CDS             complement(1385..1600)
                     /locus_tag="VFD08_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HZK10651.1"
                     /translation="MRCIITFHTIADAMYMESLAKEAKIPGRLIPVPRELSSGCGISW
                     ASEASEEEKLKKLIELESLEIEQIVLI"
     gene            complement(1602..1811)
                     /locus_tag="VFD08_06235"
     CDS             complement(1602..1811)
                     /locus_tag="VFD08_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZK10652.1"
                     /translation="MREIDVRGLSCPEPVMQVNMALKAFPGEELKILLSEAHSYKNVV
                     EFLKDKKVPMEAKEVGLDFEVIIRP"
     gene            complement(1821..2903)
                     /gene="yedE"
                     /locus_tag="VFD08_06240"
     CDS             complement(1821..2903)
                     /gene="yedE"
                     /locus_tag="VFD08_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HZK10653.1"
                     /translation="MKNTYKLFGFGIIAGLITMALAIAGNPTNMAICIACFVRDIAGS
                     MKYHTAPVVQYFRPEIVGLVLGAFFLSLYKKEFRATGGSSPAIRFFLGVAMMIGALIF
                     LGCPTRMIIRMAAGDISAYVGFVGFILGVATGAFFLKKGFSLGRSYPVRQESGAFFPV
                     IFGLIFLMSVLVPSSFAWSEAGPGSIHAPMIISLVAGLAFGALAYHSRMCFAGGFRDV
                     ILMKNFDLFAILLGIFLVMFGYNVVTNNFAFSAFGPVAHAQTLWNILGLYIVGFAAVL
                     LGGCPARQLILAGQGSADSVVTVLGMLLGAASAHNFGLASTAAAKATADAPAALGGPG
                     PAGQIAALVSIAFLFIVAFTGIKKQA"
     gene            complement(2907..4043)
                     /locus_tag="VFD08_06245"
     CDS             complement(2907..4043)
                     /locus_tag="VFD08_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZK10654.1"
                     /translation="MIYLDNSATTTNKPAEMREAMLRSLELGNPGRGLSESALDAARS
                     VFETRKKMAEFFGGKINRTVFTYNATHSLNLALLGLISPGDRVLSSYYEHNSVLRPLY
                     SLEASGVNLKLFKPEGLSISLEDIKNNLEDDTRAVVLTHSSNVLGGLTQVREIGQYLR
                     EKGLLFILDASQTAGVFPINIEEDFIDALCFTGHKTLMGPQGTGGLILSERAEPKAVF
                     SGGTGMFSFEKGMPSHLPDRLEAGTLNGVGIAGLGGSLDYLKKVDIDLLRKKEQDLAL
                     DFYDGAKDIDGIKIYSERPEKGAAIVTLNIGDMDSGHVSDLLTRCYSIATRPGAHCAP
                     LVHELLGTVEQGAVRFSFSSYNNKEEVAAALEALKEISAYRNED"
     gene            complement(4709..5611)
                     /locus_tag="VFD08_06250"
     CDS             complement(4709..5611)
                     /locus_tag="VFD08_06250"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HZK10655.1"
                     /translation="MSGVCKESKKVKFEFIRNEAGDHSVKLLCKLLNVSRAGYYAFRH
                     RPPSQTALHHQWLTGEIKRVFDEHKGRYGSPRIAQQLYEEGIETNKKTVAMLMQRAGL
                     VSVYSRKRRKKQHHASKEGVLHDNLLKRDFNSSKPGMIFVTDITYVPCSDGRLYLTPY
                     LDLATRLPKSYGITEHMRKSCVIEPLERLLRQGDAKEGAMTHSDQGSQYRSYAFAALC
                     EENGLRQSMSAAGKPIDNAVAEAFFKTVKTEVIKPNRHLTKAQMEVVLRNYLDDYYPN
                     KRIHTTLGMTPLQYEQQLLTKMVT"
     gene            complement(5578..5865)
                     /locus_tag="VFD08_06255"
     CDS             complement(5578..5865)
                     /locus_tag="VFD08_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZK10656.1"
                     /translation="MRKTYPKDFKIQICQHILTGQKTVGEISKEYGIARPIVSRWLAE
                     YKRYGNNAFAGKGNRLPEAARIYALEKENEQLKEELTILKKFQEFAKNQKR"
     gene            5932..6438
                     /locus_tag="VFD08_06260"
     CDS             5932..6438
                     /locus_tag="VFD08_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZK10657.1"
                     /translation="MSKEAKSRRSYSKEFKESVLQRLEPPTNDTVTSLSKELNITRGS
                     IYQWIRAKENQPKEFKPASKWSSKDKFHAVLETATLSQEELAAYCRRKGLYVEDLDIW
                     REQCIMANMRDAKDPLKLEEDLQKEKDRSKELEKELRIKEKALAETAALLVLRKKAQA
                     IWGDPEVD"
     gene            6414..7091
                     /locus_tag="VFD08_06265"
     CDS             6414..7091
                     /locus_tag="VFD08_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK10658.1"
                     /translation="MGGPRGRLISGPNRAKAVALIDQARAAGARLKSACTILGISERT
                     YQRWTKDGLIREDQRPIAKRPTPKNKLTKEEREEVVKTVTTPEFADLTPHQIVPKLAD
                     EGRYIASESTIYRILKEERMNTYRGRAQTPVKRKIPTHIAVGPNQVWTWDITWLNAFI
                     KGQYYKLYLILDMFSRLIVAWEVWETEKSQYAEQLIRWESHEGGRIPSYLRKVRSSKF
                     LFKTQGK"
     gene            7288..7443
                     /locus_tag="VFD08_06270"
     CDS             7288..7443
                     /locus_tag="VFD08_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10659.1"
                     /translation="MENRIEVYEKARAKHPENWSKNIRGWSLPKYVALNPLKDEELVL
                     ALKEVAK"
     gene            7665..7916
                     /locus_tag="VFD08_06275"
                     /pseudo
     CDS             7665..7916
                     /locus_tag="VFD08_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2336 a         1605 c         1821 g         2154 t
ORIGIN      
        1 atactcacta agagtattag tcgatttggt agggacacag tggatacact tgaagcgtta
       61 aaccagctaa aaactttagg ggtgcgagtt atatttgagc aagaagattt ggatacttca
      121 aatacagata gcgaccttat gatctctatc atcgaagcta tagcacagtc tgaaaatgaa
      181 tctcgtagtg aaaatattaa atggggaata aaacagcgtg ctgcacaagg cacttcaaaa
      241 ctatataacc gaaaatgtta tgggtatgta catgataaag agggcaatct gattattaaa
      301 gacgatgagg ctaaaaatgt aacgaaaata tttgagatgt acctccatgg gaaaagtatt
      361 attggtatag ttgctgagtt ggaacggttg ggtgttaaat ctcctactgg aaaagataag
      421 tggagcaagc gtactattga tgtgatgctt agcaacgaaa aatatacggg tagtgtaaga
      481 ttattggata atggaaaaca tgaagtggtt tatttatgtg aggataataa cccggccatt
      541 atttctaagg aaacatttca agcggtacag atagaaaaaa ctaaaagaag caatgtcatt
      601 aaaggcgaaa atggagtaca aagaaaaagt aaaaaatata gttcgaaaca gtaaatttcg
      661 aaaatagcct aattacaaat tgtaagtcga gaagataata tagatttaga acctttataa
      721 acactagagt tttgttatat tttataaaag agtgaaatag cctaacaatt tcaccttgtg
      781 ggaagccagc ttatgaatgg cattgggggg tctggagatt ttgctagaaa tggctacctc
      841 tcaatttttt cggctccttc tactgcccta gggggcaagg tgtccaccat tgtgcccatg
      901 gtttcccatg tagaccacac agaacatgat gtggatataa taattactga gatgggtcta
      961 gctgatctta gggggaaaac ttctgtggag agggcggtgg agattattga aaactgttcc
     1021 catccaaact acagggacca tctctactcc taccttgaga gagctgtaaa aagagggggt
     1081 catgagcccc atcttctaga agaagccctg agctggcatg tcaacttcat ggagagggga
     1141 agctcccttt tctagttcaa aaaaagagat ctttgcttta aagttaggcc tatggtatga
     1201 tttattgaac aagtggagtc tttgatggag gagatatgct ggaatatttt aagttttttg
     1261 ccaagaaggt acctccctga atgatgatct atgtcctgct gggagcagta tacctcttag
     1321 ttctactagc taagtttatc tttaagttct agacaagaaa aatccctcac tggagggatt
     1381 ttgtctatat tagtacaatt tgctcaattt ctaagctttc aagctctatg agctttttta
     1441 atttttcttc ctcactggcc tcgctggccc aggagatacc acagcctgag gagagctccc
     1501 tggggacggg tatcagcctt ccagggattt tagcttcctt agctaggctc tccatataca
     1561 tagcatctgc tatggtgtgg aaggttataa tacatctcat gctagggcct gattattact
     1621 tcaaagtcaa ggccaacttc tttagcttcc atggggacct ttttgtcctt tagaaattca
     1681 actacatttt tatagctgtg ggcttcgctg agaaggattt ttagctcttc accaggaaag
     1741 gcctttaggg ccatgtttac ttgcattaca ggttctggac aggatagtcc tctaacatca
     1801 atttctctca tatttttctc ctatgcttgt tttttgattc cagtaaaggc gacgatgaat
     1861 aaaaaggcta tagacactag agctgcgatt tgacctgcag gtcctggtcc gcctagggct
     1921 gctggtgcgt cagctgttgc ctttgctgca gcagtagagg ccaggccaaa gttatgggca
     1981 gaggctgctc ccaggagcat acctagaaca gtgactacag agtctgctga gccttggcct
     2041 gctaggatta gctggcgagc tgggcaacct ccaaggagga cagctgcaaa gccgacgatg
     2101 taaagaccaa gtatgttcca cagggtctgg gcatgggcaa cagggccaaa ggcgctaaag
     2161 gcgaagttat ttgtaactac attgtagcca aacatgacta gaaagattcc cagtaggatg
     2221 gcaaaaagat caaagttttt catgaggatg acgtctctaa atccacctgc aaagcacatc
     2281 ctagagtggt aggctagagc accaaaggca aggcctgcca ccagggaaat aatcatagga
     2341 gcatggatgc taccagggcc agcttcactc caggcaaagg atgaggggac taggacactc
     2401 attaaaaaga taagaccaaa gattacaggg aaaaaggcac cgctttcctg cctcactggg
     2461 tagcttctac caaggctaaa gcctttttta agaaagaagg ctcctgtagc tacacccagg
     2521 ataaagccta caaagccaac ataggctgag atgtctcctg ctgccattct aattatcatt
     2581 cttgtggggc agccaaggaa gattagggcg ccaatcatca tggcaacgcc gaggaaaaac
     2641 ctaatggctg gcgaggagcc tcctgtggct ctgaactctt ttttataaag agacaggaag
     2701 aaggcaccta ggaccaggcc tacaatttca ggacgaaagt actggacgac aggtgctgtg
     2761 tggtatttca ttgagcctgc aatatctcta acaaaacagg ctatacagat agccatgttg
     2821 gtagggttac ctgctatggc tagtgccatg gtaattaggc cggcaatgat gccaaagcca
     2881 aagagtttgt atgtattttt cattaattaa tcctcatttc tataggctga tatttccttt
     2941 agggcttcta gggcggctgc tacttcctct ttattattgt aggaggaaaa ggagaacctg
     3001 actgctccct gttccaccgt tcccaggagc tcatggacca gaggagcgca gtgggctcct
     3061 ggtcttgtgg ctatagagta gcatctagtc agtaggtctg agacgtggcc tgagtccatg
     3121 tctcctatgt ttagggtgac tattgctgct cctttttcgg gtctttcact gtagattttg
     3181 attccgtcaa tgtcctttgc cccgtcataa aagtctaggg caaggtcttg ctcttttttt
     3241 ctaagtaggt ctatatccac ttttttaagg tagtctaggg agccacctag gcctgctatt
     3301 cccaccccgt ttagggttcc ggcttccagt ctatcgggta ggtgactggg catgcccttt
     3361 tcaaaggaaa acatgcctgt acctccgcta aagactgctt tgggttcggc tctttcggag
     3421 aggatgagtc cgccggttcc ctggggaccc atgagagtct tgtggccagt aaaacagagg
     3481 gcgtcgataa agtcctcctc aatatttatg gggaagaccc cagcagtctg ggaggcgtca
     3541 aggataaaga gcaggccttt ttctctaagg tactggccta tttcccttac ctgggttagg
     3601 ccccctagca cattggagct gtgggttagg accacagccc tagtatcatc ctcaaggttg
     3661 ttttttatat cctctaggct gatagagagg ccctccggtt taaagagctt taggtttaca
     3721 ccgctagcct ctaaactgta gaggggtctc agtactgagt tgtgttcata gtaggagcta
     3781 agaaccctat ctcctgggct aattagcccc agtagggcaa gatttagact gtgggtggca
     3841 ttataagtaa agacagtcct gttgattttg cccccaaaaa attccgccat tttttttctt
     3901 gtctcaaaga cgctcctagc tgcatccagg gcgctttcag aaagccctct accggggttt
     3961 cccagttcaa ggcttcttaa catggcttct cgcatttctg caggtttatt tgttgttgtt
     4021 gctgaattat ctaggtagat catcctagcc cctttcttga caataaaagc taacacctga
     4081 ggtgtgactt ggcactgctt tgatgtaaaa ccaggggcct attaaatctg tggaaggggt
     4141 aaaaactagg caattggttt ttaaccctct atagaataaa gaaaaagcct tctgggaatc
     4201 taatcctaag aaccctagaa gaccaaagaa aagtctaaat agagcaagat gaaaggatta
     4261 gtgcatcaaa actatcttca tgccatgaag tgaggctttt gcgtcaagtg aagaaattgc
     4321 atagatggct catgggtgag cttttacttg taattaatcc catcattatt actcctttcg
     4381 ggaaaacttt atcacagagg gcttgggacg tcaacacctt atcggacgaa aagttaagct
     4441 accttttcta aagccttaca ctgcaggctg agaagaaatt catttggact gagataccct
     4501 agtgcctact gctttcgctc actattgtaa aaactataaa tatgctcaaa gatactttga
     4561 cgggcctact ctctagtttt aaagttagtc ccatggacca ccccctatgt gttaggatag
     4621 ttgtcgcatg aagaaaaaag tgtacactat taataaggac aaacactagg gagaataatg
     4681 gatatacaac ccttttcttt gcatgaaatt aagtgaccat tttagtgagt aattgctgtt
     4741 catactgcaa cggcgtcatt cctaaagtag tgtggattcg cttgttcggg tagtaatcat
     4801 ctagatagtt acgaagaacg acttccattt gcgctttggt aagatgacga tttggcttaa
     4861 tgacttctgt tttaacagtt ttaaagaaag cctctgctac agcattgtca ataggctttc
     4921 cagcggcact catggactgt cttaagccgt tctcttcaca aagagctgca aaggcataac
     4981 tgcgatactg acttccttga tcactgtggg tcatggcacc ttccttagca tcaccttgtc
     5041 gaaggagacg ctccagtggc tctatgacac aactttttct catatgttcg gtgattccat
     5101 aggacttagg aagtcgagtc gccaagtcaa ggtagggtgt taaatacaat cttccatcac
     5161 tgcagggaac ataggtgatg tctgtcacga agatcatacc aggtttagag ctgttgaaat
     5221 ctcgcttcag aaggttatcg tgaagaaccc cttctttcga tgcatggtgc tgctttttgc
     5281 gacgtttgcg gctataaaca ctaaccagtc cagcacgttg catgagcatg gccactgttt
     5341 tcttgttggt ttcgatgcct tcttcataga gctgctgggc aatacgcgga gagccgtagc
     5401 gacctttgtg ctcatcgaac acacgcttaa tctctcctgt tagccactga tgatgtaaag
     5461 ctgtttggga gggaggacga tggcgaaatg cgtaatagcc agcgcgacta acattcagga
     5521 gtttgcaaag gagctttacg ctgtggtctc cagcttcgtt gcggataaac tcaaacttta
     5581 ccttttttga ttctttgcaa actcctgaaa tttttttaga atggttagct cctccttgag
     5641 ctgttcgttt tccttttcta gtgcgtagat gcgtgcagcc tctggtagtc ggtttccttt
     5701 accagcgaag gcattattac catagcgttt atactcagct agccagcgag aaacaattgg
     5761 acgtgcaatc ccgtattctt tagaaatctc tccaactgtt ttctggcctg taagaatgtg
     5821 ttgacagatt tgaatcttga agtctttcgg ataggtcttt cgcatgatgg gcctctttct
     5881 ttaaccggta acttaactgt gtgttaagga aagtgtagca tatccataaa tatgtcaaaa
     5941 gaagcgaaaa gcagaagaag ttattcgaaa gaattcaaag aatcagttct acaaagactg
     6001 gaacctccaa ctaatgatac agtaactagc ttatcaaaag agctaaacat aaccaggggc
     6061 agcatttacc agtggattag agccaaagaa aaccagccca aggaatttaa accagcatcc
     6121 aaatggtctt caaaggacaa attccatgct gttttagaaa ctgccaccct aagccaagaa
     6181 gaattagcag cctattgccg caggaaaggt ctatatgtag aagatttaga catttggcgt
     6241 gagcagtgta ttatggcaaa tatgagggat gccaaggatc ccttgaagct tgaggaagat
     6301 ttacaaaaag agaaagatcg ctccaaagag ctagaaaaag agctaaggat aaaagagaaa
     6361 gcactggcag aaacagcagc tcttttagta ctaagaaaaa aggcccaagc gatttggggg
     6421 gaccccgagg tagactaatc agtggcccaa atcgcgcaaa agcagtagca ctgattgatc
     6481 aggcaagggc agctggagct agattaaaat ctgcctgtac catcctaggt attagcgagc
     6541 gcacatacca gagatggaca aaagatggcc tgattagaga agatcaaaga cccatagcta
     6601 aaaggcccac gccaaaaaac aaactaacaa aagaagaacg cgaagaggta gtaaaaacag
     6661 ttaccacccc tgagtttgct gatttaacac cccatcaaat agtacccaag ctagctgatg
     6721 aaggaagata tatcgcatca gagtcaacca tctataggat tttaaaagaa gaaagaatga
     6781 atacctatag aggtagagcc cagacgccag taaaaaggaa aattccaaca catatagcag
     6841 tgggccccaa tcaagtttgg acttgggata tcacctggct taatgccttc atcaaaggtc
     6901 agtattacaa gctttacctg atattagata tgtttagccg tttaatagta gcctgggaag
     6961 tatgggaaac tgaaaaatcc caatatgccg aacaactgat ccgatgggag tcccatgaag
     7021 gcggtcgcat tccaagctac cttagaaaag ttaggagttc aaagttcctt ttcaagaccc
     7081 agggtaagta atgacaaccc ctattctgaa tcacttttta aaactttata gtactgccct
     7141 aaataccctt tcaaagggtt tggcagtata gaagaggcca gaaaatgggt taagaggttt
     7201 gtggattggt ataacctagt ccatttgcac agtggaatca attacctcac tccataacaa
     7261 aggcactatg gacttgataa aaaaatcatg gaaaaccgga tagaggttta tgagaaagct
     7321 agggctaaac acccagaaaa ttggtcaaaa aacatcaggg gttggtcact cccgaaatat
     7381 gttgcattaa accctttaaa ggatgaagaa cttgttttag ccttgaaaga agtagctaaa
     7441 tagaggaatt tttgaaaacg taatatttgg ccagcgatcc atttatattg agaagccggc
     7501 atgatagcct atcaagaaag gcagggcata attttctaga tacccttatg cacaataatt
     7561 atgccagtag aggctcaatg taaatggcaa atcgcgggga tgtacaaacc tgtggaaaaa
     7621 atttaatgcg acaacttgct tgacacatac cgtttcctgg tgtgtcttgg cagcgatgtc
     7681 aggtacactt cctaggaaac atcttatcca gcatccttaa aaaagactca aaagccttta
     7741 gggaagagct caaagtcatc tttcttttaa ctgacatcga agctgctaga agggccaaga
     7801 atcatctgct tatagactag gagggtgagc ctaggtatga gaaggcctgt atcactctgg
     7861 acgagggctt tgaggatgct tttcagtatg ctgtaaatgg tacagctcac ccgcgc
//
LOCUS       DASRNA010000228         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_746211,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000228 DASRNA010000000
VERSION     DASRNA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746211"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..389)
                     /locus_tag="VFD08_06280"
     CDS             complement(123..389)
                     /locus_tag="VFD08_06280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZK10660.1"
                     /translation="MDNIGLIGMTSIIISGITMALGSIAPALGQGQAVSQALRSIAQQ
                     PDESNTITRTLFVGLAMIESTAIYCFVIAMILLFANPFWNLVVG"
     gene            complement(405..1085)
                     /locus_tag="VFD08_06285"
     CDS             complement(405..1085)
                     /locus_tag="VFD08_06285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZK10661.1"
                     /translation="MNIDPSQIVYFEWGFVRITATLVFTWVLMLILILVSYLVTRTIK
                     PGTEVSKGQNILEAVIETINEQIRQISQEDPGRYLPFIGTLFIFILASNVLSIVPGFK
                     PPTGSLNTTIALSLCVFLAVPFYGITDRGLLGYMKEYLKPTFIMLPFNIISEISRTLS
                     LAIRLYGNVMSSSVVVAILLGVVPLFFPIVIQALGLLTGVIQAYIFAILAIVYIASST
                     KKKTLDTE"
     gene            complement(1090..1368)
                     /locus_tag="VFD08_06290"
     CDS             complement(1090..1368)
                     /locus_tag="VFD08_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZK10662.1"
                     /translation="MMISFLSGLFIGVIYFGGLYYSTHKIKAVRNPGLFMALSLLIRM
                     GILLVGLYYLAQRGYKSILLGFIGIMLIRFILVFQVRKHSSNSNSERK"
     gene            complement(1383..1685)
                     /locus_tag="VFD08_06295"
     CDS             complement(1383..1685)
                     /locus_tag="VFD08_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZK10663.1"
                     /translation="MGEEKRTPEEELLEEVEKDVAKKIKAEKEGKEIMFGLGFFGIVG
                     WSITVPTLLAIALGVYLDKRFSSNFSWTLTLIFVGVILGSFNAWRWLNDNSQDREK"
     gene            complement(1700..2089)
                     /locus_tag="VFD08_06300"
     CDS             complement(1700..2089)
                     /locus_tag="VFD08_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZK10664.1"
                     /translation="MRLKIMLPYKTAFDKEIYQITAPGNEGEFQVLPKHLDGTWILKA
                     GVLVIGLDGDDKEEIYFAISQGMLVKEGSNLYLPCLQAIEGESLESLTKRVRKDLEVF
                     NESEKKAKRALLRLETDTVKRFMDFSK"
     gene            complement(2079..>2241)
                     /locus_tag="VFD08_06305"
     CDS             complement(2079..>2241)
                     /locus_tag="VFD08_06305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720041.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZK10665.1"
                     /translation="IEGRLVDLEDALDGCERILKDEFKDYDETDLYLIGTIEEAVEKR
                     NKRGADNET"
BASE COUNT          708 a          468 c          349 g          716 t
ORIGIN      
        1 ttcttactct tctgtcttcc atggctttta atacgggtcc ataaaaaagc ttatttaaga
       61 taaataagag cagaaaaaaa ttaataattt gcgctacaat ttcaaatatg ctaatttcca
      121 tcttagccta ccactaggtt ccaaaaggga ttagcaaaca acaaaatcat agcaataaca
      181 aaacaataga tcgcagtaga ctctatcatg gccaggccaa caaaaagagt tctagttatg
      241 gtatttgatt catctggttg ttgggcaatg gatcttaaag cctgagatac tgcctggcct
      301 tgacctaggg caggtgcaat agaccccaaa gccatagtga ttcccgaaat aataattgat
      361 gtcataccaa ttaaaccaat attatccatt aaattcctcc ttttttattc cgtgtctaaa
      421 gtctttttct ttgtacttga tgctatatag acaatcgcaa gaattgcaaa tatataggct
      481 tgaatcacac cggttaagag ccctaatgct tgaatgacaa tcgggaagaa aagtggaaca
      541 actcccaata atatggctac tacaacacta gaactcatta catttccata taaacgtatg
      601 gcaagggata aggttcgtga tatctcgctg ataatgttaa agggcaacat aataaaggtt
      661 ggtttgagat attctttcat atagccaaga aggcccctat ctgttattcc ataaaatggg
      721 accgccaaaa atacacacag ggataaggca attgtagtat ttaaagagcc tgttggtggt
      781 ttaaagcctg ggacaattga caaaacattt gatgctagga taaatataaa caaggtgcca
      841 ataaaaggca gatatctacc gggatcttct tgacttattt gtctgatctg ttcattaata
      901 gtttctatta ctgcttccag tatattttgc cctttagaga cttctgtgcc tggtttaatg
      961 gttctagtaa ccagatagga taccagaatt aatatcaaca tcaaaaccca agtaaaaacc
     1021 agtgtggctg ttattctaac aaatccccac tcaaaatata cgatttgact tggatcaata
     1081 ttcatatgat cacttccttt ctgagtttga attagaagag tgttttctta cttgaaatac
     1141 cagtataaat cgaatcagca taattcctat aaatcccaat aagatactct tatagcctct
     1201 ttgcgccaaa taatataaac caactagcaa aattcccatc cgaatgagta aagataaggc
     1261 cataaaaagc cctggatttc taacagcctt gattttatgg gtactatagt agagaccccc
     1321 aaaataaata actccaataa ataaaccgct taaaaatgaa atcatcatat tctctccaat
     1381 ttctattttt ccctgtcctg actattgtca tttagccagc gccaagcatt aaaagaacct
     1441 aaaataactc ctacaaagat aagagttaag gtccaggaaa aattggatga gaatctttta
     1501 tctaaataaa cacccagtgc tatggctaag agtgttggta cggtaataga ccaaccgaca
     1561 atgccaaaaa aacccaagcc aaacattatt tcttttcctt ctttttcggc cttgattttt
     1621 tttgctacgt ctttttctac ttcctctaaa agttcttctt ctggagttct tttttcttcg
     1681 cccacaatat cacttccttt tatttgctaa aatccataaa tctcttcaca gtatcggttt
     1741 ctagtctgag tagggctcgt tttgcttttt tttcactttc attaaagact tctaaatcct
     1801 tacgaactct cttggtgaga ctttctaagg actctccttc tattgcttgc aaacagggaa
     1861 gataaagatt gctgccttct tttaccagca ttccttggct tatggcaaaa taaatttctt
     1921 ctttatcatc cccatctagc ccaatcacca aaacaccggc ttttagaatc caggtgccat
     1981 caaggtgttt aggcagaacc tgaaattccc cttcgtttcc aggggctgtg atttgataga
     2041 tttctttatc aaaggctgtt ttatagggaa gcataatttt aagtctcatt atctgccccc
     2101 cgcttatttc ttttctcaac tgcttcttcg attgtgccaa ttaaatataa atcagtttca
     2161 tcgtagtctt taaattcatc ctttaaaatt ctctcacaac catccagggc gtcttctaaa
     2221 tccaccagtc ggccctctat c
//
LOCUS       DASRNA010000229         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_221515,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000229 DASRNA010000000
VERSION     DASRNA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221515"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      81..274
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            374..1294
                     /locus_tag="VFD08_06310"
     CDS             374..1294
                     /locus_tag="VFD08_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK10666.1"
                     /translation="MKRNVLIALVLSILMLFTACQSTSNTELPDTDRAGYAIELPQKV
                     ETIVSLAPSTSQVIESLGLKDKLIAVDSQTPFYLEGVDQLPQFDLMSPDLEALAALNP
                     DLIFTTGMSSLDGDDIFSQLKDLGISIATIPSSESLEDIQLDIQFIADALGLSEKGEE
                     ISAQMQKDIDVIADMAKEIKEPKTVLFEIAALPDIYSFGSSTFLNELLEIVGAENIFA
                     DQTSWLAVTEEAAISANPDVILTNVNYIEDSVGEILSRNGWEEVTAIKEESVYYIDNG
                     FSSLPNQNVVKALKEMALAIYPEVYASLED"
     gene            1297..2328
                     /locus_tag="VFD08_06315"
     CDS             1297..2328
                     /locus_tag="VFD08_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZK10667.1"
                     /translation="MKKPMQKIIYSLLLAILVIIISTVVGSANIPFSQIPAIFSHKLF
                     SNPLPAEIDSLLVDIFWKIRLPRALASFLVGGALAVSGTVMQSVLRNPLASSYTLGVS
                     AGASLGVALYMAFGISLPIIGNFILPFIGFSFGLATVFIAIGLSSRFNPGLENQTIIL
                     TGMVISLFMNSLLTIIMSVFREHLQRVVFWQLGSFSGLKWIELGILTLSILPIVVILM
                     RYTLELDIITFGEEHAISVGIDLKKTKLILITLATFLTGASVSFVGVIGFVDLIAPHI
                     VRRIFGSSHRLVLPLSFVFGGTFMAFADIISRLIISPKELPIGSVTALIGAPFFIWVY
                     FSKKRGVNA"
     gene            2321..3094
                     /locus_tag="VFD08_06320"
     CDS             2321..3094
                     /locus_tag="VFD08_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10668.1"
                     /translation="MLKFKNVSAGYNGKDIIKDISFNLAKGESLAIIGPNSCGKTTLL
                     RAAAGIIEHKGDIKLSGQSIKDLKRKEIAGKIALMSQINYVHFPYTVYETVMMGRYRF
                     LRQNIFGKPSKEDLAIVKKTMESTKLIDLKDKPLNVLSGGQLQRVYLAHTLVQEPEII
                     LLDEPTNHMDMKHQIDVVNFLKGWASVNNHAVIGVFHDLNLAMELTENLLFMKEGRIF
                     GKGKAKKLITSKFLKEIYDVDIVSYMTKSLGRWQEFEKE"
     gene            3098..3622
                     /locus_tag="VFD08_06325"
     CDS             3098..3622
                     /locus_tag="VFD08_06325"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HZK10669.1"
                     /translation="MKKKYRSPYEAYPFLSDPSDDLRCDFEIMTDRLASMTGLLASLC
                     PEEELKEELLRIDRLIYHLNPSLRTFFSIKEEEMQFLLERVGELEVENASLYDTFILP
                     SGSTRASLAHLLRVDSKALVRLLYRYEEKGNKIETQIFDFSNLLSGYFFHLAMKLNSL
                     DEVEEISYKSRNYK"
BASE COUNT         1126 a          657 c          689 g         1178 t
ORIGIN      
        1 gtttacctcc tttgcaaact aatactagtc taaacgaaaa tataaatcat tgcaatggac
       61 ttttagtgat taagtccttg acgaaaaaaa tcctagagag tagaatgata ttgcaattga
      121 atattttttt cagaagaggg aaggaggttt gaatcctcca cagagccgct gctgtaataa
      181 actggaaaga ctcatatcca ctggtaatac tgggaaggag agtcattcct aatagtttta
      241 agtcagaaaa cctacctggg aaaactgcaa ccaatctctt cggacctaag agaatgcatg
      301 gctttttctt attaaatgga taggctgttt tctctgggtg aagacagctt ttttatttag
      361 aaaagaggaa tgtatgaaaa gaaatgtctt aatcgccctt gtacttagta tacttatgtt
      421 gtttactgct tgccagtcaa ctagtaatac tgagcttcct gacaccgacc gagctggata
      481 tgccattgag cttccgcaaa aggtcgaaac gattgtctct ttagcccctt ctacgagcca
      541 ggttattgag tctcttggac ttaaagacaa actcattgca gtagatagcc aaacaccttt
      601 ttatcttgaa ggtgttgacc agcttccgca gtttgacctt atgagtcctg atcttgaagc
      661 actcgctgca ttaaatcctg atcttatatt taccactggt atgtcatctt tagatggtga
      721 tgatatcttt tctcaattaa aagatttagg tatttctata gccacaatcc cttctagtga
      781 aagcctagaa gatatacaac ttgatataca gtttattgct gacgcccttg gtttatctga
      841 aaagggtgaa gaaataagcg ctcaaatgca aaaagatatc gatgttattg ctgatatggc
      901 aaaagaaata aaggaaccca aaaccgttct ttttgaaatt gcagcccttc cagacatcta
      961 tagttttggc tcatctacct ttttaaatga actgcttgaa atcgttgggg cagaaaacat
     1021 atttgcggat caaactagct ggcttgccgt tactgaagaa gctgctattt ctgcaaatcc
     1081 agatgttatt ttaaccaatg taaattatat agaagattct gttggtgaaa ttctatccag
     1141 aaatggatgg gaggaggtca cagcaatcaa agaagaatct gtttactata ttgataatgg
     1201 ctttagcagc ctgccaaacc aaaatgtagt aaaagcacta aaagaaatgg cactagccat
     1261 ttatcctgag gtttatgctt ctttagagga ttaattatga aaaagcctat gcaaaaaatt
     1321 atctacagcc tacttttggc aatattagta ataatcattt ccacagtagt tggatcagct
     1381 aatattccat ttagtcagat tccagccatt ttttcgcata agcttttttc taacccactt
     1441 ccggctgaaa tcgatagcct tttagttgat attttttgga aaatccgcct cccaagagcc
     1501 ctggcctcct ttttagtcgg gggcgctcta gctgtaagtg gaactgttat gcaatcagta
     1561 ctaaggaacc ctctggcttc atcttatacc ctaggtgttt ctgcaggagc ctctcttgga
     1621 gtggctcttt atatggcttt tggtatttct ttaccgatta ttggtaattt tattttacct
     1681 tttatcggct ttagttttgg cctagccaca gtttttattg ccataggact ttcttcccgg
     1741 tttaatccag gtcttgaaaa tcaaaccatt atcttaacgg gtatggtaat ttctcttttt
     1801 atgaactctc ttttaaccat tattatgtct gttttccgag agcatctaca aagagtggta
     1861 ttttggcagc taggcagctt ttcagggctt aaatggattg agcttggaat tcttactcta
     1921 agcattttgc ctatagtagt aatactaatg cgctacacct tagagcttga tatcataact
     1981 tttggggaag aacatgctat ttctgtaggt attgatctta aaaaaacaaa attgatattg
     2041 attaccctgg ccacatttct tactggtgcc tcggttagtt ttgttggagt tataggtttt
     2101 gttgatctta ttgctcccca tatagtaaga aggatatttg gttcctccca tagacttgta
     2161 ctgcctttat cctttgtctt tggaggaact tttatggctt ttgcagatat tatttcccgg
     2221 cttattatct cgccaaaaga gctccccatt ggctcagtaa ctgcacttat cggtgcgcct
     2281 tttttcatct gggtatactt cagcaagaaa agaggtgtca atgcttaagt ttaagaatgt
     2341 cagtgccgga tataatggta aagatataat taaagacatc tcctttaatc ttgcaaaggg
     2401 tgagagtcta gcgattatag gaccaaatag ctgcggtaaa actacacttt taagagcagc
     2461 agcaggaatt attgaacata aaggagatat aaagctatca ggtcaatcaa ttaaagattt
     2521 aaaaagaaaa gaaattgcag gcaaaattgc actgatgagt cagattaatt atgtacactt
     2581 tccctataca gtttatgaaa cagttatgat gggtcgatac cgctttctac gacaaaatat
     2641 atttggcaaa cccagcaagg aagacctcgc catagttaag aaaacaatgg aaagtacaaa
     2701 gctaattgac cttaaagata aaccccttaa tgttctatct gggggtcagc tacaaagggt
     2761 ttatttggct catactttag ttcaagaacc cgagattatt ttacttgatg aaccaaccaa
     2821 ccatatggat atgaaacatc agattgatgt cgttaatttt ctaaaaggct gggcttcagt
     2881 aaacaatcac gctgttatcg gtgtttttca tgatcttaat cttgctatgg aattaactga
     2941 aaatcttctc tttatgaaag aaggtaggat ttttggaaag ggaaaagcaa aaaaactgat
     3001 tactagcaaa tttctaaaag aaatctatga tgttgatatc gtatcctata tgacaaagtc
     3061 cttaggtcgc tggcaggagt ttgaaaagga gtaaaatatg aagaaaaaat accgcagtcc
     3121 ctatgaagcc tatccctttt tatctgatcc ttctgatgat ttgcgctgtg attttgaaat
     3181 aatgacagat cgccttgctt ccatgacagg cctattggca tccctatgtc ctgaagaaga
     3241 attaaaagaa gaactcctaa gaatagatag attaatttat catttaaatc ctagtcttag
     3301 aacatttttt agtattaaag aagaagaaat gcaatttctt ctagaaaggg taggggagct
     3361 agaggttgaa aatgcctcct tatacgatac ttttattcta ccctcaggat ctacaagagc
     3421 aagcctagcc catctactgc gagttgattc taaagcccta gtgcgtctcc tatatcgata
     3481 cgaagaaaag ggaaacaaaa tagaaactca aatctttgat ttttcgaatt tactctcagg
     3541 atatttcttt catcttgcca tgaaattaaa ttctttggat gaagtagaag agatttcata
     3601 taaaagtcga aattataaat aattgttttt acttggctca tacacaaaag
//
LOCUS       DASRNA010000230         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_147018,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000230 DASRNA010000000
VERSION     DASRNA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147018"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFD08_06330"
     CDS             complement(<1..1175)
                     /locus_tag="VFD08_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HZK10670.1"
                     /translation="MKKLRFYLALIAARFTTFILKLTGHKATHTPGAVALRLCPDFLI
                     HLSKPKTLIAITGTNGKTTTANLIADLYTNLGIPFAHNSYGSNIKEGVVTTLLDASSF
                     FGGPKLNLCILEVDERASRIIFGELPPDYLLVTNLFRESYLRNAHGEFIFRVLQEHIP
                     ENTHLILNSDDLISSRLGGNNPRTYYSIAQLAHEKSQGGSLIEDVPLCPLCHQPMVYS
                     FRRYHHIGKAHCSSCSFTNFKADFVLKDVNKCFSINYKGVVERYPLLGENVLDHYAQL
                     CALSLMRTLNFTQKDLAQASKGLEIVKSRKDILQLQGKTVYRMLAKGMNPIAISRAIE
                     IILSQEGKKALILLNDTSKKSSSQEENTAWLYDTDFELLKDKDLVQILVGGYRAKDY"
     gene            1492..2778
                     /locus_tag="VFD08_06335"
     CDS             1492..2778
                     /locus_tag="VFD08_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZK10671.1"
                     /translation="MKINNYTIVDVETASRRTPSICSLGLVHVSGGKIVASHHYYIKP
                     PGRFEYWNIKIHGIEPYKVKTAPRFAQIWPEIKKYCENSIIIAHNASFDLGQISASLQ
                     EAKIAHPDFYYIDTVKMAHRVFPDFPKYNLAYLSLVMDVDLGNHHDALSDATATHGIF
                     DLMQEEYPLQRSNISIYRGSRVKTLKRSKFKGSFDQILKILRNYQADEVLKDETIEEI
                     KNWLNEVEEFKRFHPVSELLPRLNFLLQRGVYDEGVYWIIQRLAMNYYREKKRIEPNI
                     ALKELLVYLKSNDSEKISFWLEENFQLRDEFLFNELWRALWEFHFHKLGKIEELISKT
                     SFYLRPYDDIYSKEKVAGKKGMIVGSLSPKKMKYLYHELDQVGASLGGNNPRKVDFLL
                     LAPGKTGLDYTPRLRKILKLKEKGGEFTLLKVGSRK"
     gene            2775..3767
                     /locus_tag="VFD08_06340"
     CDS             2775..3767
                     /locus_tag="VFD08_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZK10672.1"
                     /translation="MKKLITILVLLVLLTSCQAQEYPSRSIQIIVPYSAGGTTDLVAR
                     AFAQVLSAEIGEEVTVVNQAGASGSVATLSAYQAAHDGYTLLFSADSLGIQRVMDLTE
                     LSFDDFEPIMLVSNDPKIIVANAKGPYQNAQDLFSAMRDQGLSMSYTGPGGSGHVQAL
                     LYQQLGAKLTLTPFDGGREAIVALLGEQVDFTNSNASVVWQFVESGDLKALAVCGNTP
                     LSLDPSIPLLGDEVEGAGELMDLAFTPLSLLVGKDTPAEVVEMLREASKKAVKNPQWI
                     EYMEESGQEKLYTKYPEPEQMRAFFQDFESKMSWLLHDAGVSSYSPEEFGIKRP"
     gene            3764..4141
                     /locus_tag="VFD08_06345"
     CDS             3764..4141
                     /locus_tag="VFD08_06345"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HZK10673.1"
                     /translation="MKKFPIGIFALVLCVYGLYLVQVSDIELALSPGLFPLLLGLFML
                     IVGGGISFQRSKEKTPLVLPGLIAAYTGLWFVVGPRISTFAFAGFSLYILKEESLSRS
                     LLFGLLLTLFIDLLFARFFGIQF"
     gene            4149..>5229
                     /locus_tag="VFD08_06350"
     CDS             4149..>5229
                     /locus_tag="VFD08_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZK10674.1"
                     /translation="MLFLYTFIGSLLGLVVGTIPGLSVTMATALLLGLTYSWPTHHAL
                     ATIMGVYVVGVFSGAISAILINVPGAPSALVTTLDGHPMAKKGEGADALFLAVIYSFV
                     GSLFGLLALALFAKPLSIFALSFRTIDYFLLAFFALSALMSLSGGKPKKAILAGVAGI
                     LFSLVGLDPITGTHRFTFGIRLLMPGIPLVPVLLGFFGLGEAIMMLEQKDKTRFKLNK
                     REIKAKEALSYFPFSLYYALLGTLVGILPGAGTPVASFLSYSQAAKRTKNPKVPFGQG
                     AREGIIAPEAANNACVGGALIPMLSLGIPGDGVSAIILSVFMVHGLKPGPLFMMENYD
                     TFLWILKSGLVASVFLLILGLSLAPY"
BASE COUNT         1513 a          983 c         1139 g         1594 t
ORIGIN      
        1 taataatcct tagccctata accccctaca agaatctgaa caagatcctt atccttgagc
       61 agttcaaaat ccgtgtcata aagccaagca gtattctctt cctggctgct ggattttttg
      121 cttgtgtcat taagcagaat aagggctttt ttaccttctt gagataggat gatttctata
      181 gccctgctaa tagcaatggg gttcattcct tttgctagca tacggtagac ggtcttgccc
      241 tgtagctgta agatatcctt gcggctttta acaatctcaa gaccctttga agcttgggcg
      301 agatcctttt gcgtaaaatt taaggtacgc attaggctaa gggcacaaag ctgggcatag
      361 tgatctagga cattttcccc cagcaggggg tagcgctcta ccacgccctt gtagttgata
      421 gaaaaacact tatttacatc tttaagcaca aaatcagcct taaaatttgt aaaggagcaa
      481 gaagagcagt gggcctttcc aatatggtgg tatcgcctaa aactatagac catgggctgg
      541 tggcataagg gacaaagagg gacatcctcg attaaacttc ccccttggga tttttcatgg
      601 gctaactggg caatggaata ataggtcctt gggttatttc cccccagtct agaactaatt
      661 aggtcatcgc tattaagaat caaatgagtg ttttcaggga tatgttcttg taggacccta
      721 aagataaatt caccatgggc atttcttaga taggactccc taaaaagatt ggtaacaaga
      781 agataatccg gaggcagttc gccaaagata attcgacttg ctcgctcgtc cacttctagg
      841 atgcaaagat taagtttagg tcctccaaaa aaagaagaag catccaagag tgtggtgacc
      901 accccttctt ttatattgct gccatagcta ttgtgggcaa agggtattcc tagattagtg
      961 tataagtcag cgataagatt agctgttgtg gttttaccat tagtacccgt aatggcaata
     1021 agggttttgg gcttggacaa atgtattaaa aaatcaggac acaatcttag agcaacagct
     1081 ccaggagtgt gagtggcctt gtgccctgta agtttaagga taaaagtagt aaaacgagct
     1141 gcgataaggg ctaaataaaa acgcagtttt ttcattgatt cctctttcta taagtttaaa
     1201 ccatataaat tatagcatag ctttcctagt agcttagcag ataaatatgt ttattttatg
     1261 tttattcaga tcaacaagca ataatgataa aagtctggga ttttttcttc ttttatcatg
     1321 atggggctat ttaagctggt ataccactag ataaataagc taaaatcatg aaatttatag
     1381 atggacattg gaagagaggg ctagatttgt tatactatca gtgttggcat aagaaaatct
     1441 acctggctta gaagaaaaga agaacttata ggtagataca aaggaatgat tatgaaaatc
     1501 aataattaca cgatagttga tgttgagaca gctagccgcc gcacacccag tatctgttct
     1561 ttagggcttg tgcatgttag tggaggaaag attgttgcaa gtcatcatta ttatataaaa
     1621 cctccagggc gttttgagta ttggaatatt aaaattcacg ggattgagcc ttataaggta
     1681 aaaacagcac caagatttgc tcaaatctgg ccggaaataa aaaagtattg tgaaaacagc
     1741 attattattg cccataatgc aagttttgat ttagggcaga tttctgcctc tttacaggaa
     1801 gcaaaaatag cccatcctga tttttactat atagacacag taaaaatggc ccaccgcgtt
     1861 tttccagatt ttccaaaata caatcttgct tatcttagtc ttgttatgga tgtagatcta
     1921 ggaaatcatc atgatgcctt aagtgatgct acggcaaccc atggcatctt tgatctaatg
     1981 caagaagaat accccttgca aagaagtaat atctccattt atagaggaag tagggtaaaa
     2041 acactaaagc gcagtaaatt taaaggaagt tttgatcaaa tactaaaaat tctaagaaat
     2101 tatcaagcag atgaagtcct aaaggatgaa acaatagaag aaattaaaaa ttggctaaat
     2161 gaagtagaag agttcaaaag atttcatcca gttagcgagc ttcttccaag attaaacttt
     2221 cttttgcaaa gaggtgtcta tgatgaagga gtttactgga ttatacagcg actcgcaatg
     2281 aattattata gagaaaagaa aagaatagag ccaaatattg ccttaaagga gctgctagtt
     2341 tatttaaaat caaatgactc tgaaaagata agtttctggc ttgaggaaaa ctttcaactc
     2401 cgcgatgaat ttctttttaa tgaactatgg agagctttat gggagtttca ctttcataaa
     2461 ctaggtaaaa tagaagagtt aataagcaaa acgagttttt accttagacc ctatgatgat
     2521 atttattcaa aggaaaaggt tgcaggaaaa aagggcatga ttgtgggaag cctgtctccc
     2581 aaaaagatga agtatcttta tcatgaactt gatcaggtag gggctagcct gggaggaaac
     2641 aatcctagaa aggtagattt cctacttctt gctcctggaa aaacaggact ggattataca
     2701 cctaggctaa ggaaaatttt aaaattaaag gaaaaaggag gggaatttac cctcctaaag
     2761 gtaggtagca gaaaatgaaa aaacttatta cgatactagt gcttttggta cttttaacat
     2821 cttgccaagc acaggaatat ccttctagaa gcatacaaat tattgttcct tattcagcag
     2881 ggggaacaac agaccttgtg gcccgagctt ttgcccaggt tcttagcgca gagataggag
     2941 aggaagtaac tgttgttaat caagccggcg cttcgggttc ggtagctact cttagtgcct
     3001 atcaagcggc ccatgatggc tatacattgc tttttagtgc agattctctg ggcattcagc
     3061 gggtgatgga tttgacagag cttagctttg atgattttga accaattatg cttgtaagca
     3121 atgatcctaa aatcattgtg gcgaatgcca aggggcccta tcaaaatgcc caggatcttt
     3181 tctctgccat gagagaccag gggctaagca tgagttatac aggccctgga ggctcaggac
     3241 acgtccaggc cctgctttat cagcaactag gtgctaagct aaccctaacc ccctttgatg
     3301 gtggacgtga agcgattgtg gctctcttag gagagcaggt agactttaca aactctaatg
     3361 catcggttgt ttggcaattt gttgaaagtg gagatttaaa ggctcttgca gtttgtggta
     3421 atacacctct gtccttggat ccctccatcc cccttttagg ggatgaagtg gaaggagccg
     3481 gggagcttat ggatctggcc tttactcccc ttagtcttct tgtgggaaaa gacacgccgg
     3541 cagaagtggt tgaaatgcta cgtgaagctt ccaaaaaggc agttaaaaac ccccaatgga
     3601 tagagtatat ggaagaaagc ggacaagaaa agctttatac aaaatacccc gagccggaac
     3661 agatgcgggc gttttttcaa gactttgaat ccaaaatgag ctggctcctg cacgatgccg
     3721 gagtgagttc ctatagtcca gaagaatttg gcattaaacg tccatgaaaa agttccctat
     3781 aggcattttt gccctagttc tttgtgtgta tggtctatat cttgttcaag tttctgatat
     3841 agagctagcc ctctcaccag gactctttcc acttcttctt ggacttttta tgctgattgt
     3901 gggagggggt atttcttttc aaagatccaa agaaaaaaca ccccttgtct tacctggctt
     3961 aattgctgct tatacaggac tctggtttgt agtagggcca agaattagta cctttgcctt
     4021 tgctggattt agtctttata ttttaaagga agaatcccta agcaggtctt tactttttgg
     4081 acttttacta actcttttta tcgatctact ttttgctcgc ttttttggaa ttcaattttg
     4141 aggtttttat gcttttctta tataccttta taggctccct tttgggacta gttgttggga
     4201 ccataccagg gctgagtgtt acaatggcta cagcccttct tttgggctta acctattcct
     4261 ggcccaccca ccacgctctg gcaaccatta tgggagtcta tgttgtaggg gtcttttcgg
     4321 gggctatttc agctatcttg attaatgttc ctggagcgcc ttcggctctt gttactactt
     4381 tagatgggca tcccatggca aaaaaaggag aaggggctga cgccttattt cttgcagtaa
     4441 tctactcttt tgttgggagt ttatttggcc tcttagccct agctttattt gccaaacctt
     4501 taagtatttt cgcactttct tttcgcacca ttgattattt tcttttggca ttttttgctt
     4561 taagtgcgct gatgtccctt tctggaggaa agccaaaaaa ggcaatctta gcaggagttg
     4621 ctggaatatt gttttctctt gtagggctag accctattac tggaacccac cgttttacct
     4681 ttgggattcg gctgcttatg ccaggcatcc cactagtgcc ggttcttctg ggtttttttg
     4741 gcctagggga agcgattatg atgttggagc aaaaagacaa aacacgcttt aagttaaaca
     4801 aaagagaaat aaaagcgaag gaggctttat catattttcc tttttctctt tactacgctc
     4861 ttctgggtac tcttgtgggg attcttcctg gtgcgggaac gccagtggct tcttttttat
     4921 cttattctca agcagccaaa cgcacaaaaa accccaaggt gccctttggg caaggagcaa
     4981 gagaaggaat tattgcacca gaggctgcaa ataacgcctg tgtaggagga gctcttattc
     5041 ccatgcttag cctaggcatt ccaggagatg gggtttctgc tattatttta tccgttttta
     5101 tggtacatgg actaaagcca ggccctcttt ttatgatgga gaattatgat acctttcttt
     5161 ggatattaaa aagtggccta gtggcatctg tgtttttact tatactaggg cttagtctag
     5221 ctccctacc
//
LOCUS       DASRNA010000231         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_816332,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000231 DASRNA010000000
VERSION     DASRNA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816332"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFD08_06355"
     CDS             complement(<1..1081)
                     /locus_tag="VFD08_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZK10675.1"
                     /translation="MEIGKKATNEDRSHSLHLKLIVFAGLLLLQIVLFVLAARYVRDY
                     FGFVLAIIPILEFIVFIYIFNDSSNPMYKLAWVFLILTIPFFGILIYLFVAFNVKQKA
                     MHQRFSTMANRYGPLYDKISNEGVHRDLKEEDGLASLTSSYLKNTGDFPVFKAQGVDY
                     HNDGRIHFDDMLVELEKAEKFIFMEYFILADGLMWQSILDILKKKVKEGVEVRLLLDG
                     MMAFINLPRDFVEEMEGYGIKARLFNVMRVNLSVHQNHRDHRKIMVIDGEVAYTGGLN
                     IADEYINEKKRFGYWKDTAVKIQGDAVDSFTVMFLEVWNVLEGGHLEERYLRESKSSL
                     AKDGFVIPYYDTPYNGEEIGKKSIWI"
     gene            complement(1053..1505)
                     /locus_tag="VFD08_06360"
     CDS             complement(1053..1505)
                     /locus_tag="VFD08_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008801688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HZK10676.1"
                     /translation="MKILISACLLGQACRYDGKAVEKIKLDGFDLYPLCPEVLGGFST
                     PRAPSERRGERVINSQGEDVTRGFVQGAQRALDLMEKEKIQIALLKENSPSCGVHFIY
                     DGNFSGKKVKGKGIFTELLLEKGYRVLSEEEIEIVKEERNGNRKKSDQ"
     gene            1851..2126
                     /locus_tag="VFD08_06365"
     CDS             1851..2126
                     /locus_tag="VFD08_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZK10677.1"
                     /translation="MKKNRHGRGRRHKCPVCPYKTIADLPLGGQATVEKLLGQGPVRR
                     RIMDMGITKGVNVKYIKNAPLGDPIELLVRGYKLSLRLEDARKIQVK"
     gene            2126..>3905
                     /gene="feoB"
                     /locus_tag="VFD08_06370"
     CDS             2126..>3905
                     /gene="feoB"
                     /locus_tag="VFD08_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZK10678.1"
                     /translation="MKLTLALIGNPNSGKTTVFNDLTGSSQYIGNWPGVTIEKKSGRL
                     LQNKGVDLVDLPGIYSLSPYSLEEVITRNYLIEEAPHAIINVVDSTNLERNLYLTTQL
                     MEMEIPTVIALNMSDLTEKYGYYIDSEKLTRAFGLPVVETSALKGTGLAELVRVSVDL
                     AASGKNTAKPIVFRTEIERALKEIQDLFPEEDHKRYRSIKLFERDEKHLEELDHPRRQ
                     KLEEILAPLEEQFDDSSDALIIADRYDEIQRRLLGVHRKEERGKTPTERIDAFVTNKY
                     LALPIFFGIMYIIYYIAMTTVGGWTVEYIENLVGYLSGNAQLLLANLGASAWAQSLVS
                     QGIIGSVGAIFAFVPQLMILFFFLSLLEDTGYMSRIAFIMDRLFRRFGLSGKSFIPML
                     IGTGCSVPGIMAARTIENPRDRKMTILLTPMIPCGAKLPIFAMFISLIFGGNPWLGPL
                     IYLLSIVTVFISGMILKRFRRFQGTPSPFVMELPAYKLPSLKNVFISMWDKALGFVKK
                     AGSIIFVSSIVLWLLQHFNWQLDYLPTQIDESILADIGELLRYIFIPLGFGDSWAPAV
                     ASISGLIAKEVVVTTFASVGSIVPISF"
BASE COUNT         1162 a          868 c          731 g         1144 t
ORIGIN      
        1 atatccagat agactttttt ccaatttcct ccccattata gggggtatca taatagggaa
       61 tgacaaaacc atctttagct agagagcttt tgctttctct aagatatcgc tcttctaaat
      121 ggcccccttc tagtacattc catacttcaa gaaacataac agtaaaacta tctactgcat
      181 ctccttgtat ttttacggct gtatccttcc agtagccaaa acgcttcttc tcattaatat
      241 actcatctgc aatatttagt ccccccgtat aggctacttc cccatcaatg accataatct
      301 ttctatggtc ccggtggttt tgatggaccg agaggttaac gcgcataacg ttaaagagcc
      361 tggctttgat tccatatcct tccatctctt ctacaaaatc tctgggaaga ttaataaagg
      421 ccatcatccc atcaagtaaa agacgcacct ctactccctc ttttaccttt ttctttaaga
      481 tatccaaaat actctgccac ataagaccat ctgctagaat aaagtactcc ataaaaataa
      541 atttttctgc tttttctagt tccactaaca tatcatcaaa atgaattctt ccatcattat
      601 ggtagtctac cccctgggcc ttaaaaacag gaaaatcacc tgtgtttttc aagtaactgg
      661 aggtaaggga ggccaggcca tcttcttctt taaggtccct atggactcct tcattactga
      721 tcttatcgta aagaggacca tagcggtttg ccatggtaga aaacctttga tgcatggcct
      781 tttgtttcac attaaaggca acaaaaagat aaataagaat accaaaaaag ggaatggtaa
      841 gtattagaaa gacccaggcc agtttgtaca tgggattact ggagtcatta aagatataaa
      901 taaaaacaat aaattctaat atagggatta tggctagaac aaagccaaag taatccctta
      961 catatcttgc ggctaaaaca aaaagaacaa tttgcagtaa tagtaaacct gcaaaaacga
     1021 taagtttcag atgcagacta tggctgcggt cttcattggt cgcttttttt cctatttcca
     1081 tttctttcct cttttactat ttctatttct tcttcgctca agacccggta acctttttct
     1141 agtagaagtt cagtaaatat tcctttcccc tttacttttt tccctgaaaa atttccatca
     1201 taaataaaat gaactccaca agaaggtgag ttttccttta aaagagcaat ttgaattttt
     1261 tctttttcca taagatctag ggccctttgg gctccctgga caaagcccct tgtaacatct
     1321 tcaccctggc tatttattac cctttctccc cggcgctcgg aaggagctct aggagttgaa
     1381 aaacctccca gcacttcagg acaaagaggg taaagatcaa aaccatccag ttttattttt
     1441 tctaccgcct tgccatcata acgacaagcc tgacccaaga gacaggcact aataagaatt
     1501 ttcatttctt caccctttcc ttcctagtat aatcgaaaaa cacaaaaggg tccataatta
     1561 atacagcagg aataggagtc tctctaagta aaaagcagtt ttatctaaaa agccccctat
     1621 aggaataata cttacaattt aacatcaaga gtgttagatt taaagtaggg agtggtagtt
     1681 taggaagtct tccccttttt tctagctcaa gcctaggtaa aaaagagcct tatctttgaa
     1741 aataacacaa gatataaata gacttgtgag gcaggcagat gacaagaagg atattgacaa
     1801 tggttctcaa ttaagataga attaaagcat tataaaaagt agaggttagg atgaaaaaaa
     1861 atagacatgg cagaggcaga agacacaaat gtcccgtatg cccatacaaa acaattgctg
     1921 atcttcccct gggtggccag gcaaccgtag aaaaactttt aggacaggga cccgttagaa
     1981 gacgaattat ggatatggga attaccaagg gtgtcaatgt aaaatatatt aaaaatgccc
     2041 ctttgggcga ccccatagag ctccttgttc gcggctacaa actctccctg cgtctagaag
     2101 acgctagaaa aattcaggtt aaataatgaa actcactctt gccctaatcg gaaatccaaa
     2161 ttccggaaaa accacagttt ttaatgacct taccggcagt agccagtata ttggcaactg
     2221 gcccggagta acaatagaaa agaaaagtgg tcgacttctt caaaacaagg gagtcgacct
     2281 tgttgactta ccaggaattt actccctctc cccctattct ttagaggagg taattacgcg
     2341 taattacctt atagaggaag ctccccatgc cattattaat gtcgttgata gcacaaatct
     2401 agagcgcaat ctctacctta ccacccagct gatggagatg gaaatcccca cagtgattgc
     2461 ccttaatatg agtgatctta cagaaaaata cggttactat attgatagtg agaagctaac
     2521 cagggccttt ggccttcctg tagtagaaac ctcagcccta aaggggacag gactcgctga
     2581 gctggttagg gtttctgttg atctagcagc ctcgggtaaa aacacagcaa aacccattgt
     2641 atttcgcaca gaaatcgaaa gggccctaaa ggagatccaa gatcttttcc ctgaggaaga
     2701 tcataagaga tatcgtagca tcaagctctt tgagcgtgat gaaaaacatc tagaagaact
     2761 agatcatcca agaaggcaaa aactcgaaga aattcttgcc ccccttgagg agcaatttga
     2821 tgatagtagt gacgccctta ttattgcaga ccgctacgac gaaattcaaa gacggctact
     2881 gggtgtccac cgaaaagaag aaagaggaaa aacacccacc gaacgcatcg atgcctttgt
     2941 taccaataaa tacctggccc tacccatttt ttttggcatt atgtatatta tttattatat
     3001 tgccatgaca accgttgggg gatggaccgt agaatatatt gaaaatctcg tgggatatct
     3061 tagtggaaat gcccagctgc tactagcaaa ccttggcgcc tctgcctggg cccagtccct
     3121 ggtttcccag ggcattatag gaagtgtggg ggccattttt gcctttgtac cccagctgat
     3181 gattcttttc tttttcctat ctcttctaga agatacaggc tatatgtcaa gaatcgcctt
     3241 tattatggac cgcctctttc gacgttttgg cctttcagga aaaagcttta tccccatgct
     3301 aattggcaca ggttgcagtg ttcctgggat tatggccgcc cgaaccattg aaaatcctag
     3361 agaccgtaag atgaccatcc ttcttactcc tatgattccc tgtggggcaa aactacccat
     3421 ctttgccatg tttatttctc ttatctttgg aggaaatcct tggctgggcc ccttgattta
     3481 tcttcttagc attgtaacag tctttatttc aggtatgatt ttaaagcgct ttcgacgatt
     3541 tcaaggaacc ccctcgccct ttgttatgga gctacctgcc tataaacttc ccagcttaaa
     3601 aaacgttttt attagcatgt gggataaggc cctgggcttt gttaaaaagg caggaagtat
     3661 tatctttgtt tcctccattg ttctttggct tttacaacac ttcaactggc agctggatta
     3721 tctaccgacc cagatcgatg aatcaatcct agcagatatt ggtgagcttc ttcgctatat
     3781 ctttatcccc ctaggctttg gtgattcctg ggcccctgcc gtagcaagta ttagtggcct
     3841 tattgccaag gaagttgtgg ttacaacctt tgccagcgtg ggtagtattg tgcctattag
     3901 cttta
//
LOCUS       DASRNA010000232         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_594754,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000232 DASRNA010000000
VERSION     DASRNA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594754"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFD08_06375"
     CDS             complement(<1..233)
                     /locus_tag="VFD08_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10679.1"
                     /translation="MKEDVISTLINRFDYSGAYVLSCDRAQDTAFLHLLDIGRSMVNF
                     DFVTAQEILTENGDLFPLYFSAYLEENLLDLIYG"
     gene            complement(202..1092)
                     /locus_tag="VFD08_06380"
     CDS             complement(202..1092)
                     /locus_tag="VFD08_06380"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZK10680.1"
                     /translation="MTVIIDKSAADQRIDRFLTKLFPRAPRGEIFRLFRKKDVRINGK
                     RASVGDRIALGDEVFVYVSQERKKSWMAKEVVLRPGQIKIIYEDKQDLVVYKQRGLKT
                     TPDRAGEDCLTYRVQAYLRDSISPTFKPSPLGRLDKETEGLVLFAKNYQRTKYLEELQ
                     RENKVKKAYLALVFGTIREGLYETSLKKHKNKPGVKVDPQGKVARTIFKNLASSPPYS
                     LVEAELLTGRTHQIRASIAALGGKIVGDELYGKGRGGQRLLCYKLEWAEKKAFYLPED
                     FLLELRKVGIDEGRCNLNTN"
     gene            1156..1662
                     /locus_tag="VFD08_06385"
     CDS             1156..1662
                     /locus_tag="VFD08_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10681.1"
                     /translation="MFEKIKDLIYDISDYLVTIIIVFFIVISITFVMTKSYGLDFSKD
                     DLLSLFQKEAMVEEEIETPASAPTEVEAETPSPELPETATPLEDLEEEEEEEEEEEDK
                     EIIVEVYPGDGSYDVGDRLVEAGIIEDSYELALTLVERNLDTALQVGQYTFTKDMTLD
                     QVIDVLFP"
     gene            1674..>2578
                     /gene="fba"
                     /locus_tag="VFD08_06390"
     CDS             1674..>2578
                     /gene="fba"
                     /locus_tag="VFD08_06390"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HZK10682.1"
                     /translation="MPLVTTKEMFEKAYKGKYAIGAFNVNNMEIIQGIVEAAKEARAP
                     LILQVSSGARKYANANYLTKLVEAALLDAPELDIALHLDHGDTFELCKQCIDDGFTSV
                     MIDGSHLPYEENVALTKQVVDYAHARGVSVEAELGRLAGVEDDINVAAEDATYTDPEQ
                     AKDFVTRTGCDSLAVAIGTSHGAYKFKGEPKLDIPRLKRIQELLPGFPIVLHGASSVI
                     PEFVDMNNEYGGDIPGARGVPEELIREATKYGVCKVNIDSDLRLAMTGVIRRELSKDP
                     GNFDPRKYLGPARSAIKEIVTHKIY"
BASE COUNT          760 a          540 c          500 g          778 t
ORIGIN      
        1 ccataaataa gatctaataa attttcttct aaataggcac taaaatacag gggaaacaaa
       61 tccccattct cggttagaat ttcttgagcg gtaacaaaat caaagttgac catacttcta
      121 ccaatatcaa gcagatgaag aaaagccgta tcctgggccc tatcacaact taaaacataa
      181 gctccgctat agtcgaatcg attaattagt gttgagatta catcttcctt catcaatacc
      241 aacctttcgt agttccaata aaaaatcttc tggcagataa aaagcctttt tctctgccca
      301 ctctaactta taacaaagta gtctttgacc ccctcgacct tttccataaa gctcatcgcc
      361 cactattttg cctcccaagg cagctatact tgctcgaatc tgatgcgttc ttcctgttaa
      421 tagctctgcc tctacaaggc tgtagggagg actgctggct aggtttttaa atatggttct
      481 agccactttt ccctggggat cgacctttac tcctggcttg tttttatgct tctttaaaga
      541 ggtctcatat agaccttctc ttattgtacc aaatacaagg gccaaatagg ccttttttac
      601 tttattctcc ctttgaagct cctctaaata cttggttctt tgatagttct tagcaaaaag
      661 aactaggcct tcagtttctt tgtcaagacg ccccaaaggc gaaggtttaa aggtgggact
      721 tatactgtct ctaagatagg cttgtaccct ataggtaagg caatcttcac ctgctctatc
      781 tggggttgtt ttaagacccc tttgcttata gacaacaagg tcttgcttat cttcataaat
      841 gatttttatt tgaccagggc gaagaaccac ttcttttgcc atccagcttt tttttctttc
      901 ttggcttaca tatacaaaga cttcatctcc tagggcaatc cggtctccca ctgatgctct
      961 tttaccatta attctgacat cttttttcct aaatagacga aatatttctc ctctaggggc
     1021 cctaggaaaa agcttggtta aaaagcggtc tatgcgctgg tctgccgcgc ttttatcaat
     1081 aataactgtc acatatactc cttatatatt gcaatatttt tgcacctatg ataaattgaa
     1141 accagaggtg atgctatgtt tgaaaaaata aaagatctta tctacgacat aagcgattat
     1201 ttggttacta tcattatagt cttttttatc gtaatatcca taacttttgt tatgaccaag
     1261 tcttatgggc ttgatttctc aaaggacgat ctgctttctc ttttccaaaa agaagccatg
     1321 gtagaagagg aaattgaaac ccctgccagt gctccgacgg aagttgaagc tgagacacct
     1381 tcacctgagt tgcctgaaac agcgactcct ctagaagatt tagaagaaga agaggaggaa
     1441 gaggaagaag aagaagataa agaaattatt gttgaagttt atcctggtga tggatcctat
     1501 gatgtaggag accgcttggt tgaagctgga atcattgaag atagctacga acttgctctt
     1561 actcttgttg aacgaaattt ggatactgcc cttcaagttg gtcaatatac atttacaaaa
     1621 gatatgaccc tagatcaagt catcgacgtt ctatttcctt aaggaggtaa tgaatgccac
     1681 tggtaaccac aaaagaaatg tttgaaaaag catacaaggg caaatatgcc attggtgctt
     1741 ttaatgtaaa caatatggaa atcattcaag gaattgttga agccgccaaa gaagctcgtg
     1801 cacccctgat tcttcaggtc tcatcaggag cgagaaaata tgccaatgct aattatctta
     1861 ccaaactcgt agaagctgct cttcttgatg ctcctgagtt agatattgct ctccaccttg
     1921 atcatggtga tacttttgag ctttgtaagc aatgcattga tgacggtttt acatcagtaa
     1981 tgattgatgg cagccatctt ccttatgaag aaaatgtcgc tctaacaaaa caggtagtag
     2041 actatgccca tgcaaggggc gttagtgtcg aagcagaatt aggccgacta gccggcgttg
     2101 aagacgacat aaatgtcgca gcagaagatg ccacctatac tgatcccgag caagcaaagg
     2161 actttgttac ccgtacaggg tgtgattctc ttgctgttgc cattggaacc tctcatggtg
     2221 cctataaatt taagggagaa cctaaacttg atatcccaag actaaagcgg attcaagaat
     2281 tacttcctgg ctttccgatt gtccttcacg gtgcgagttc agtcattcct gaatttgttg
     2341 atatgaacaa tgagtacggt ggtgatattc ctggtgctag gggagttcct gaggaactta
     2401 tacgcgaagc aaccaaatat ggagtatgta aagtcaatat cgactcagat cttcgcttag
     2461 ccatgacagg agttatccgt agagaactta gcaaggatcc gggaaatttt gatccccgta
     2521 aatacctagg cccagcacga agtgccatta aagaaatagt aacacataaa atttacca
//
LOCUS       DASRNA010000233         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_883226,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000233 DASRNA010000000
VERSION     DASRNA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883226"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VFD08_06395"
     CDS             <1..1169
                     /locus_tag="VFD08_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK10683.1"
                     /translation="IGTSIAGTSILARLLGAKETKKAEEVAAHLMALSLIFALVFSTL
                     GYKLTPMILSFMGAKGDFFLYGKVYLQIDFLSFPFVMIFFALQSLLASQGNNKAITIV
                     SSIAAITNVVLDPFFIFDEFLGFKGLGMGVAGAAYATLLSKMILTVLGFIIFYSGKTE
                     INLHLRKFKAKISVFKEILRVALPSTLGMMGSSFGFIILNAAIASYGTVTLAAFAMIN
                     RSTAIIMQPAMGIGGSLATIVGQNLGAGYLDRAKESLKKAALLSLIFTGSGSLLLFIF
                     DKEFVHVFFSSSVDPGVINEALVYLWYSLLTIPLMGFFSIFQGFFQGTGFTKYSMYMD
                     LGRLWLIRLPMIYLFKNFTEFGSQGIWFSMTSSNVLIILYAWWLYTRGRWSSSY"
     gene            complement(1203..2843)
                     /locus_tag="VFD08_06400"
     CDS             complement(1203..2843)
                     /locus_tag="VFD08_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HZK10684.1"
                     /translation="MWVTIGLGVVSGLSLLIYGIQMMSTALQKAAGNRLKEFVEFITK
                     NRFIALISGMIITIILHSSSATSVMVVGFVNSGLMTLKQAVGVIMGANIGTTVTGQLL
                     SFNLARYAPIALAVGLVLKITGKTTRRKDFAEILIGIGILFIGMGLLKDSVNPLKGYP
                     KFEEYLIQWGSNPFLGISLGVIITVLMQSSAATIALLIALGSEGILPLEAAIYILYGD
                     NIGTCTTALFSSIGSSRNARRIAFIHLLFNIIGTLIFVLVLGKPMLRFITNLNPTDVA
                     RQVANSHSLFNIINVILLFPAAGLLVKISELVIRDKDEKEESPLLDPRFLATPTIALQ
                     NTIAESVAMAKLAKRSLESAILSYKNRDKSHIDESNRLEEQINRYQHLIMAYLQELSE
                     AALSQGDRRVVDALFNTVSDIERIGDHAENIAQLSNDYIDGNVEFDPEATKEMRAIID
                     HVLHTLTIIINAMRLDDVELAKEVMELEDQMDEMEKVSQQNHIDRMHTKEATIQSGVI
                     FLDLLSNLERVLDHANNIAQSIIELQGDMNSIFTHKDD"
BASE COUNT          892 a          588 c          629 g         1038 t
ORIGIN      
        1 gtataggaac aagtattgcc ggtaccagta ttcttgctag gctccttggc gccaaagaga
       61 caaaaaaagc agaagaggtt gccgcccacc ttatggctct gagtttaatc tttgccctag
      121 tgttttctac tcttggttac aagttaactc ctatgatctt atcttttatg ggagcaaagg
      181 gagatttttt tctctatggc aaggtgtatt tgcagattga ttttctatcc tttccctttg
      241 taatgatttt tttcgcccta caaagtcttc ttgcttccca gggaaataat aaggccatca
      301 cgatagtcag ctccattgcg gccattacca atgtggttct agatcctttt tttatttttg
      361 atgaattctt aggattcaag ggtcttggaa tgggtgtagc tggtgctgcc tatgccactc
      421 ttttaagtaa gatgattctt acagttcttg gttttattat tttctactca ggaaaaactg
      481 aaattaacct gcaccttaga aaatttaagg caaaaatttc tgtctttaaa gaaattctac
      541 gggtagctct tccttcaacc ttgggtatga tgggttcttc ttttggattt attattctta
      601 atgctgctat tgcttcctat ggcacggtga cccttgctgc ctttgccatg atcaataggt
      661 ctacagcaat tattatgcag cctgcaatgg gcatcggtgg aagtctggct actattgtag
      721 gtcagaacct gggggcgggt tatcttgatc gggccaagga atccctcaaa aaagctgcac
      781 tattgtccct tattttcacc ggatctggaa gtctgcttct ttttattttt gataaagaat
      841 ttgtgcatgt gtttttctct agcagcgttg atccgggcgt aataaatgag gccctagtat
      901 atctttggta ttcccttctt actattcctc ttatgggatt tttcagtatt tttcaaggat
      961 tttttcaagg aacaggcttt accaaataca gcatgtacat ggatttagga agactttggc
     1021 ttattcgcct acctatgatt tatttattta agaattttac tgaatttggt tcccagggaa
     1081 tctggttttc catgacttct agcaatgtcc tcattatact ttatgcctgg tggctttaca
     1141 caagaggaag atggtcttct tcctattaaa aaagaggagg tcttaaggga ccccctcttt
     1201 ttttaatcgt ctttatgcgt aaaaatcgag ttcatatctc cttgaagttc aataattgat
     1261 tgagcaatat tgtttgcgtg gtcaaggact cgctctagat tgcttaagag atctaggaag
     1321 attacaccac tttgaatcgt agcttctttt gtgtgcatac gatcaatgtg attttgttgg
     1381 cttacctttt ccatttcatc catttggtct tcaagctcca ttacttcctt tgctagttcc
     1441 acatcatcaa ggcgcatggc attgataatt atagtaagtg tatggagaac atggtcgata
     1501 atggcccgca tttcttttgt tgcttcggga tcgaactcta cattaccatc gatatagtca
     1561 tttgatagtt gggctatatt ttcggcatgg tccccgatac gctctatgtc actaacagta
     1621 ttaaaaagag catcgacaac ccgtctatcc ccttgggata aggcggcctc agaaagttct
     1681 tgtagatagg ccattataag atgttgatat ctattgatct gctcttctag ccgattgctt
     1741 tcatcgatat ggcttttatc tcgattctta taggataaga tagcagattc caaacttctt
     1801 ttagctaatt tagccatggc cacactttca gcaatggtat tttgtagagc gattgttgga
     1861 gtagctaaaa atcgcggatc taaaagagga ctttcttctt tttcgtcttt atctcgaata
     1921 acaagctctg agatttttac cagaagacca gctgctggga aaagaagaat cacattgata
     1981 atattaaata gggagtggct attggccacc tggcgtgcta catcagttgg gtttaaattg
     2041 gtgataaacc ttagcatggg tttacccaga acgagcacaa atattagggt acctataata
     2101 ttaaagagta gatggataaa ggctattcgt cttgcatttc gcgaagaacc gatacttgag
     2161 aatagggctg tagtacaagt gcctatatta tcaccataaa ggatgtatat ggcagcctcg
     2221 aggggcaata taccctcgct tccaagggcg ataagaaggg ctatggtagc tgctgaggac
     2281 tgcattaaaa cagtgataat aacacctagg gaaataccta agaagggatt agagccccac
     2341 tggattaagt attcttcgaa cttgggatag ccttttaatg gatttaccga atccttaagt
     2401 aatcccatac caataaaaag aatccctatg ccgataagta tttcagcaaa atcttttcgc
     2461 cgagttgttt tccccgtaat ttttaaaaca agaccaacag ctagcgcaat gggagcataa
     2521 cgggctaaat taaagcttag aagctggcca gttacggtag ttcctatatt tgcacccatg
     2581 ataacgccaa cggcttgttt tagggtcata agtcccgagt ttacaaaacc taccaccata
     2641 actgaggtag ctgatgatga gtgaagtata attgtaataa tcattcctga tataagggcg
     2701 atgaatctat tttttgttat aaactccaca aactctttta gacgatttcc cgcagctttt
     2761 tgtaaggctg tagacatcat ctgaatccca taaatcagca gactgaggcc tgatacaacg
     2821 ccgaggccga tggttaccca catagaaatt cctcctttgt ttttcctatg aacagtgtac
     2881 cataatatcc ttttatatgg cgaatattta gcatttgtat aataaaaaaa catttataga
     2941 agaactaaaa agaacaatgt gtaaaaataa ttgcaattta atctaataaa gatcaaatga
     3001 acagataaag gggccattgt tttatgattc aaccatataa gggaagcaag ttgtctttat
     3061 agaagataca taagtagata gttctagatt tttcgtatat aatagggacg gaggttatta
     3121 tgaaaaaaag atataaaatt ataagtt
//
LOCUS       DASRNA010000234         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_816706,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000234 DASRNA010000000
VERSION     DASRNA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816706"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VFD08_06405"
     CDS             <1..157
                     /locus_tag="VFD08_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZK10685.1"
                     /translation="FLPLTLFVGWYGMNFKYMPELGWRYGYLGFGAFILLTSGLILFY
                     LRKRKLL"
     gene            complement(170..1927)
                     /locus_tag="VFD08_06410"
     CDS             complement(170..1927)
                     /locus_tag="VFD08_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK10686.1"
                     /translation="MKKYSNKDLLLSFIPYYKPYLGILSFDLLCASMTTVADMVLPLI
                     LRFMTNSVLEGQMLSYGTIARLTLFFLVLKIIDLFAGYYMAETGHIMGAKIETDMRRD
                     VFHHLQDLSTEFFNEAKVGQIMARITSDLFDITEFSHHCPEEYFIGFIKMTFAFIILL
                     RINIPLTLAIFILIPLMIFSASRFNKRMRRNFKSQRDHIGELNSRIEDTLLGVKVVKA
                     FTNEDMEKAKFEKDNLTFLDIKKDTYKSMAGFHTVTKAYDGLMYIVVIALGGTLLVQG
                     KIDSGDFVAYIMYVGTLLTTVRRIVEFTEQFQRGMTGIERFNEIMDTDIDIFDEEDAR
                     EILNPQGSLEFKNVNFGYEEESGKVLKNINIKVKAGENVALVGPSGGGKTTLCHLIPR
                     FYEVEEGEILLDGTNIKDFTIKSLRQAVGLVQQDVYLFGGSVYDNILYGNPQASFEEV
                     EEAARLAGAYDFISQLPQGFDTYVGERGVKLSGGQKQRISIARVFLKNPPILLLDEAT
                     SALDNTSERLVQKSLERLSQGRTTLTIAHRLSTIQNADRILVLDEGEIIEEGTHGELL
                     EKKAAYYKLHQLSFPEEAI"
     gene            complement(1908..2723)
                     /locus_tag="VFD08_06415"
     CDS             complement(1908..2723)
                     /locus_tag="VFD08_06415"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZK10687.1"
                     /translation="MITMEQIQELLINWVPRLLFFLIVLYVGFFLIKRFINLLAKRLI
                     VKKVDLTALGFFMSILNYFLKGIVIVISLSILNVPMASLTAVIGASTLSIGLALQGSL
                     ANLAGGLVLVTTQPFKVGDYIESDGFGGTVEEISVLTTRLNTVDGKRVVIPNSNISSK
                     GLINYSPNALRRIDLPVLLTYEASIEKAKSVFQGLIEEIEGAERSEILLTKLADSGYE
                     FTLRFWIPTPEYFKQLFILNEKISPALEEAGLELAYPHLQLVTPGDDDEKIQQ"
     gene            complement(2785..>3286)
                     /locus_tag="VFD08_06420"
     CDS             complement(2785..>3286)
                     /locus_tag="VFD08_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK10688.1"
                     /translation="LTEAPYENVRCTKEIFEDARKNYPHMLAIAHVMKEEEIAMAMAD
                     PLVMVASDGIYRDHRGHPRGAGTFPRFISRYLRDEKVMDFVQGMKKITLDPARLLGLE
                     DKGSLEIGKDADLCIFDLDKIRDRADFRDGQRPPEGISYVFIGGQRALEEGRIIRKNL
                     GRFIKR"
BASE COUNT          905 a          765 c          675 g          941 t
ORIGIN      
        1 atttctaccc ttaactcttt ttgttggctg gtatggaatg aactttaaat atatgccgga
       61 actgggttgg cgctatggtt acctaggttt tggagccttt atccttctta ctagcggtct
      121 cattctcttt tatctaagaa aaagaaaact cctgtaaaaa gaaaccatct taaatggctt
      181 cttcaggaaa acttagttgg tgcagcttat aataagctgc ttttttctct aggagttccc
      241 catgggtccc ttcttctata atctccccct catctaggac caggatgcgg tccgcatttt
      301 ggatggtgct cagtctatgg gcaatggtta gggtggtcct gccctggctt agtcgctcca
      361 gacttttttg caccagtctt tcactggtat tatcaagggc cgaagtagcc tcatcaagca
      421 gcaaaatggg tgggtttttt agaaaaaccc tagcaatact aatacgctgc ttttggcccc
      481 ctgagagctt aacgccccgc tctcctacat aggtatcaaa accttgggga agctggctaa
      541 taaagtcata ggcgccagcc aggcgggctg cctcctctac ctcctcaaag cttgcctggg
      601 gatttccata aagaatatta tcatagacag aaccaccaaa aagataaacg tcctgctgga
      661 ccaggcccac ggcctggcgc aggctcttta tggtgaagtc ctttatattg gtgccatcaa
      721 gaagaatttc accctcctcc acctcataaa aacgaggaat cagatggcaa agggtggtct
      781 tgcccccgcc actggggcca accagggcca cattttctcc cgcttttacc ttgatattta
      841 tgttttttag aaccttgccg ctttcttctt catagccaaa gtttacattt ttaaattcta
      901 agcttccctg gggatttaga atttccctgg catcctcttc atcaaaaata tcaatatcgg
      961 tatccataat ttcattaaag cgctcaattc ctgtcatccc cctttggaac tgctcagtaa
     1021 attctacaat acggcgcact gtggtaagaa gggttcctac atacataata taggccacaa
     1081 aatctcccga gtcgatcttg ccctggacta gaagggtgcc tcctagggca atgaccacaa
     1141 tatacattag gccatcatag gcctttgtta ccgtatgaaa acctgccata cttttatagg
     1201 tatccttttt aatgtccaaa aaggttaaat tgtctttttc aaattttgcc ttctccatat
     1261 cttcattggt aaaggccttt accaccttga ctcctagcag ggtatcttca attctagaat
     1321 taagctcccc tatatggtcc ctctgggact tgaaatttct tcgcatgcgc ttattaaagc
     1381 gcgaagcact aaaaatcatc aggggaatca gaataaaaat agccagggtc aagggaatat
     1441 tgatccgcag aagaataata aaggcaaagg tcattttaat aaagccaata aagtattctt
     1501 cgggacagtg gtggctaaac tctgttatat caaaaaggtc ggaagtaatc ctggccataa
     1561 tctggcccac cttggcttca ttaaaaaact ctgtagataa gtcctgaaga tgatggaaaa
     1621 cgtcccttct catatcggtt tcaattttgg cccccataat atgaccggtt tctgccatat
     1681 agtagccagc aaaaagatca ataattttta ggaccaaaaa gaaaagagtc agtctagcaa
     1741 tggtcccgta ggatagcatc tggccttcta ggacagaatt ggtcataaag cgcaagataa
     1801 gaggcaggac catatctgcc actgtagtca tagaggcgca gagtagatca aaacttaaga
     1861 ttcccagata gggcttatag tagggaataa agctaagtag caggtcttta ttgctgtatt
     1921 ttttcatcat cgtctcctgg tgtaacaagt tgtaggtggg gataggctag ttcaagcccc
     1981 gcctcttcta gggcagggga aattttttca ttaagaataa agagctgctt aaaatactcc
     2041 ggtgtgggaa tccaaaagcg cagggtaaat tcatagccgc tatcggctag tttagttaaa
     2101 aggatttcac ttctttctgc cccttcaatt tcctctatta ggccttgaaa aacactcttg
     2161 gccttttcaa tggaagcctc ataggtaaga agcacaggta ggtctatcct gcgcagggca
     2221 ttgggagagt agttgattag gcccttggag ctgatattgg aattaggaat gaccactctt
     2281 tttccatcca cggtattaag tctagttgtc agcacagaga tttcttctac cgtgccccca
     2341 aaaccatcac tctcaatata gtctcccacc ttaaagggct gggttgtcac taggacaagc
     2401 ccgcctgcta gattggccag actcccctgg agggccaggc caatggatag ggtagaagcc
     2461 ccaattaccg ctgttagaga agccatgggc acatttagga tgctcaggct aataacaata
     2521 acaatgccct ttaaaaagta gtttaggatg ctcataaaaa agcccagggc tgttaaatct
     2581 acttttttta ctatcagtcg ttttgctaac aagttaataa atcgcttaat taaaaaaaag
     2641 ccgacataaa gtactataag aaaaaaaagg agccgaggca cccagttgat aagaagttct
     2701 tggatctgtt ccatagtaat cataattata ccgcctttcc taagatattg tagcacacct
     2761 gccattaaaa atgagccctt aggctcatct tttaataaat cttcctaaat tttttcgaat
     2821 gatcctgccc tcttctaggg ccctttggcc tccaataaag acgtagctga tcccctcagg
     2881 gggcctttgg ccatccctaa agtcagcccg gtctcttatc ttatctagat caaaaatgca
     2941 aaggtctgca tccttgccaa tctcaagact tcccttgtct tctaggccca agagcctggc
     3001 aggatccagg gtgatttttt tcataccctg gacaaaatcc atcacctttt catcgcgaag
     3061 atagcgggaa ataaagcggg gaaaggtacc cgctccccgg ggatgacccc tatggtctct
     3121 atagattcca tcacttgcta ccattaccag agggtctgcc atggccatgg caatttcctc
     3181 ttccttcatt acatgggcaa tggccagcat atgggggtaa ttttttcggg catcttcaaa
     3241 gatttctttt gtgcagcgca cattttcata gggggcctct gttagc
//
LOCUS       DASRNA010000235         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_969179,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000235 DASRNA010000000
VERSION     DASRNA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969179"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1251)
                     /locus_tag="VFD08_06425"
     CDS             complement(127..1251)
                     /locus_tag="VFD08_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HZK10689.1"
                     /translation="MENTKKWPVVLLAIIIIFISNRLLVDKDFLFQGEAYEHGSAKVV
                     EVIGEKEDTTLAATTQGGMTATEVSFSAQYKGNLVLASQHVSNLIPDEKKVSPGDRII
                     LVELPGPDGQNALVMSQYNRLPAIIVIGLVFSALLILLGLKTGLDSLIALVLSIAMIF
                     FVFIPMILQGKNIYLWTLLTALYSILLTIYMITGWSKKSLVTMLGCAFGTLIAAALSY
                     GFSEYLHLTGGYDDATLALHRLRDVFQVDLRALVFAGITIGALGAVMDVAMDISSSLY
                     EIRLHAKVSFKELFTSGLTIGKDIMGTMTNTLILAYIGGSLSAVLMRVISSSSLLLLF
                     NMEIIVVDLLQALLGSLALLLTAPLTAFFAALFYSKIDPI"
     gene            complement(1255..>2703)
                     /locus_tag="VFD08_06430"
     CDS             complement(1255..>2703)
                     /locus_tag="VFD08_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10690.1"
                     /translation="FLLIGLLVFTGLRLIFGPRINYTSFKISVIFLVFLLVIPFVFSI
                     ISSPIFRADSYSKLLVPETGVFEDDIEKISLSQVPIVDRETASIIGAKQLGSLSDLVS
                     QFEIDTQYSQINLAGKPIRVSPLKYFDIIKYITNRKDGLGYYVSVDMTSQEGSIVKLN
                     KPIFYSSGDYLMRNIYRNIRFKYPFSLLGETNFEIDDEGNAHYITPLLKKTIGFFGGT
                     DAKGAIITDANSGESTFYETGDIPHWVDRVFPADLIISQLDSYGLYKGGYFNSIFGQK
                     NVTSTTSGYNYISIGEDIFLTTGVTSVRSDDSNLGFYYTNMRTKETKFYPLPSATETA
                     AMSSAQGKVQEKEYRPTFPVLLNISDRPVYFMSLKDDAKTAKMYAIVDARQFTNVYAA
                     ESVDELFREYAKAHLSAGVQQDMETVTIKDVREITLDGNSVFYFTIKENNNVYEVSAS
                     TLGPEILSLKEGSKLKILASLREDIYIVESLE"
BASE COUNT          851 a          526 c          555 g          771 t
ORIGIN      
        1 ctatgggagc gaataccttg tccatatttt tgccatgaca tcgcgtagtt gctaagcaag
       61 gcctgggagg ctttgggggt ggactggaac taaaagaaaa acttcttcaa ttagaaaaat
      121 agatccctag atgggatcta ttttactgta aaaaagagcg gcgaaaaagg ctgttaaggg
      181 agcagtaaga agaagagcca gactaccaag aagggcctgg agtaaatcta ccacaataat
      241 ttccatgtta aatagaagaa gcaggctgga tgagctaatc acacgcataa gtacagcgct
      301 taaagatcct cctatataag ccaagattag agtatttgtc atggttccca taatgtcttt
      361 accgattgta aggccactgg tgaagagttc tttaaagctg actttagcgt gaaggcgaat
      421 ttcgtagagg gaggatgaaa tatccatggc cacgtccatt acagcgccta gagccccaat
      481 ggtaattcca gcaaaaacaa gagctctaag atctacttga aagacatccc ttagcctatg
      541 tagggccagg gtagcatcat catagcctcc tgttaggtgt agatactcac taaatccata
      601 ggaaagagca gcagctatca aggtcccaaa agcacagccc aacatggtta ccaggctttt
      661 tttactccac ccagtaatca tatatatggt taggagtata ctgtaaagag ctgttaagag
      721 ggtccaaaga tagatatttt tcccctgtag gatcatgggg ataaaaacaa agaaaatcat
      781 agctatactt agaaccagcg caataagtga atccaggcct gttttaagac ccaggaggat
      841 aagtaaagct gaaaaaacta ggccaattac aataatggct ggcagccggt tatactgact
      901 cattaccaga gcattttgtc catcgggtcc aggaagttcc accagtataa tccggtcacc
      961 tgggcttacc tttttctcat cagggataag attggagacg tgttggctag caagtactaa
     1021 gttgccctta tactgggcgc taaagcttac ctcagtagct gtcatccccc cctgggttgt
     1081 ggcagctaga gttgtatctt ctttttctcc tataacttcg acgaccttgg cgctgccgtg
     1141 ctcataagct tctccttgaa aaagaaaatc tttgtctaca agaagtcggt tggagataaa
     1201 aatgataata atggccaaaa gaacgacggg ccattttttt gtattttcca taaattactc
     1261 caaactttcc acaatatata tatcttcacg tagtgaagct aatattttaa gcttgcttcc
     1321 ttcttttaaa gaaagaatct ctggaccaag tgttgaagcc gaaacttcgt agacattatt
     1381 gttttctttg atagtaaagt aaaagacact atttccatca agagtgattt cccgtacatc
     1441 ttttattgta acggtctcca tatcctgctg cactcctgct gaaagatgag cttttgcata
     1501 ttctcggaag agttcatcta cactctcagc ggcatagaca ttagtaaatt gtctggcatc
     1561 gacaatggcg tacatctttg ctgttttggc atcatcctta agactcataa aatacacagg
     1621 tcgatcagaa atatttagca gtactgggaa ggtaggtcta tattcttttt cttgcacctt
     1681 accttgggca ctgctcatgg ctgctgtttc tgtggccgag ggtagaggat aaaactttgt
     1741 ttctttagta cgcatattag tatagtagaa accaagatta gaatcatcac tacgcacgga
     1801 ggttactcct gtggtaagaa agatatcttc ccctatagat atatagttat atccactcgt
     1861 tgtagaggta acattttttt gtccaaagat agaattaaaa taaccaccct tatagagtcc
     1921 ataactatcg agttgactta taattaaatc tgctggaaaa acacggtcta cccagtgagg
     1981 aatatctcct gtttcataga aggtactttc tccgctattg gcatcagtaa tgattgcccc
     2041 ttttgcatcg gtaccaccaa aaaagcctat agttttttta agaagaggcg tgatatagtg
     2101 ggcattgccc tcatcatcta tctcaaaatt tgtttctcca agaagagaaa agggatattt
     2161 aaaacgaata ttccggtaga tatttctcat aaggtagtct ccagaagaat agaagatggg
     2221 cttattgagt tttacaatag aaccttcttg tgaagtcata tcaacactga cataataacc
     2281 aagtccatcc ttgcggttgg taatgtattt gatgatatca aaatacttaa gaggagaaac
     2341 acgaatgggt tttcctgcaa ggttgatttg gctgtactgg gtatcaattt caaactggct
     2401 tactaaatca gaaagagaac ctagttgttt tgctccaatg atactggcgg tttctctatc
     2461 gacaatgggc acctggctta gtgatatttt ctcgatatca tcttcaaaga cacctgtttc
     2521 aggaactaga agtttggaat agctatctgc cctaaaaata ggagaagata taatgctaaa
     2581 gacaaaggga ataacaagta aaaagaccaa gaagattaca cttattttga aggaagtata
     2641 atttatcctg ggaccaaaaa taagccgaag gccagtaaag accaaaagcc ctattaatag
     2701 gaa
//
LOCUS       DASRNA010000236         5309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_884070,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000236 DASRNA010000000
VERSION     DASRNA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884070"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFD08_06435"
     CDS             complement(<1..760)
                     /locus_tag="VFD08_06435"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZK10691.1"
                     /translation="MDISKRQKRSIASAANKSDDYKEILEDKEVDIVIELMGGLEPAT
                     SMIKAAMDRGKSLVTANKHALYEGQGDLESYGAKKKVGLSYEAAVAAGIPILDIVRNR
                     LFFEEIEEITAILNGTTNYILSRMAQGMPFEQALEKAGDMGYLEADPSLDLEGYDSLH
                     KIGILYNLAFGSYPTSIKREGIKRSTEIKTSNKLKLIARARPSGVSVALEEVDPGSPF
                     YPIDDGMNAIKFKTKYTKELFFSGPGAGGEATALS"
     gene            complement(685..897)
                     /locus_tag="VFD08_06440"
     CDS             complement(685..897)
                     /locus_tag="VFD08_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10692.1"
                     /translation="MKKITLALLGYGVVGQALEKLYLEEKEKAREKYMRLSGQEIDFD
                     LKWIFLRDKKGQLPQLQIKVTIIKKY"
     gene            951..1973
                     /locus_tag="VFD08_06445"
     CDS             951..1973
                     /locus_tag="VFD08_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HZK10693.1"
                     /translation="MLKAILKKTQNYLIKVLSGMALGLFSSLIIGLIIKQVGLLLNWP
                     LLETIGVYAQHLTSGAIGAGVAYSIGAPPLAIFSSIIAGILGGGALVMEEGALILSIG
                     EPVGAFVAALAGAEMGRWVGKKQKLEIILVPFSTIVVGGLVGHYVAPIVSGFMIGLGI
                     FINFATTQKPFTMGILLSLVMGIALTLPISSAALGIGLGLSGLAAGASTVGCASHMIG
                     FAVASYRDNGFSGFLSQGLGTSMLQMPNIIKKPMIALPAIVTSIVLGPISTLVFKMKG
                     TPVGSGMGTSGFVGQFATLEAMGPSSALPIFLMHFLLPGLLSLVVSEWMRKKGLIKAG
                     DMKLTL"
     gene            complement(1963..2760)
                     /gene="proC"
                     /locus_tag="VFD08_06450"
     CDS             complement(1963..2760)
                     /gene="proC"
                     /locus_tag="VFD08_06450"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZK10694.1"
                     /translation="MTIGFIGLGNMGLAMLKGIRSKREDKLIYTVKSKESAQKLEEKL
                     GFGPSSLEEIYQSDIIFLAIKPKDYPFFLQELATLLTKNQIIVSMAPGLSLEKMQSYF
                     TKKTKIVRSMPATPAQIGMSVSGLCFSQALEEEEKDLIREIFQSFGWCHDMDESLMEA
                     FGSLCGVMPAFMMNLIEAMADQAVAWGIPRNQAYILAGQIMKGSASLLLETGLHPAEL
                     KDQVTSPGGTTIEGILALEHWGGARAIQKAMQASYAKGEAMEKASRE"
     gene            2895..4091
                     /locus_tag="VFD08_06455"
     CDS             2895..4091
                     /locus_tag="VFD08_06455"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZK10695.1"
                     /translation="MNVLVINCGSSSLKFQLLNMENESLLASGLVERIGISGSQMILK
                     TRDEEDHVVKVPMKDHSIALKIVLDALVDPSYEIIKDLKEINAVGHRVVHAGEFFSKS
                     VIIDKEVIQAIEDCVELAPLHNPPNLLGINAILELMPGVPNVAVFDTAFHQTMPPEAF
                     IYPLPYEWYKDLKIRRYGFHGTSHKFVAEKAADFLGKDIQSLKIITCHLGNGSSITAV
                     ENGKSVDTSMGFTPLEGLVMGTRVGFLDPAIISYLEKKTDYTLDEITHILNHNCGVLG
                     ISCLSSDFRDLGDAAATGNEQAQLALDIFRRTVKKKIAAYAAVMGGVDAVVFTGGIGE
                     NGIEDRAGILSGLEFMGLYLDEENNKVRGETKSISTNDSPVSILVIPTNEELAIAKDT
                     KLLVEA"
     gene            4106..5188
                     /locus_tag="VFD08_06460"
     CDS             4106..5188
                     /locus_tag="VFD08_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZK10696.1"
                     /translation="MLFFIMKLLIIIAEYNPFHQGHKYHLSKAKKETGASHSLVLMGG
                     NFLQRGDVAIEDKFIRAKKALDGGADLVAELPFVYASSWAREFAQGGVAIANKIPLPA
                     TLSFGSETGSISQLKNLLAKIDQIEKDKEHSYGDLVRKNLICPPKGANDILGLEYLRA
                     LKDTKSIHKAHTVLRLGQAYHDDKQSTFPSASSLRRQIKEGEIPHDNPLFLEDFSDYI
                     YSALLTKDLLPTYGMIEGLENRLLKELGPNISLEEYIDRLSTKRYRPARLRRLLIHHL
                     IGYTKLDHKKLKDVAYLRPLAYTKKGTQILSMVKNTPLKIILPHERQGDPLISRSLEL
                     DLLASRVYNFKAGLSPGDFKRNLLHR"
BASE COUNT         1439 a         1204 c         1075 g         1591 t
ORIGIN      
        1 cacttagtgc tgtggcctct ccccccgctc ccgggcctga gaaaaacaat tcttttgtat
       61 attttgtttt aaattttatg gcattcatgc catcatcaat ggggtaaaaa ggactccctg
      121 ggtctacttc ttctagggcc acactaacac cactgggcct tgcccttgca atcaatttga
      181 gtttattgga cgtttttatt tcagtggacc ttttaatgcc ttctcttttt atagaagtag
      241 gatagctacc aaaggctaaa ttgtacaaaa tccctatctt atgaagacta tcatacccct
      301 ctagatcaag gctagggtcc gcttcaagat agcccatatc cccagctttt tcaagggctt
      361 gttcaaaggg cataccctgg gccatccggc ttaggatgta atttgtcgtg ccatttaaaa
      421 tagcggtaat ctcctctatt tcttcaaaaa agagtcggtt tcttacaata tccaaaatag
      481 gaatccctgc tgctacggca gcctcatagc tgaggccgac tttctttttt gccccatagg
      541 actccaggtc cccctggcct tcataaaggg cgtgtttatt ggctgtaaca agggattttc
      601 ccctatccat tgctgctttg atcatgctgg ttgctggctc aaggccaccc attaattcga
      661 ttactatatc cacttctttg tcttctaata tttctttata atcgtcactt ttatttgcag
      721 ctgaggcaat tgaccttttt tgtctcttag aaatatccac ttaagatcaa agtctatttc
      781 ttggccactt aggcgcatgt atttttcccg ggccttttct ttttcttcta aataaagttt
      841 ttctagcgcc tgtcctacta ccccataacc caataaggca agagtaattt tcttcattag
      901 taaatccttt catgttacaa tgctattaga attttacttg gaggaaaaca atgctaaaag
      961 caatcttgaa aaaaacacaa aactatttga taaaagttct ctcaggtatg gccctgggac
     1021 ttttttcatc tctgattatc ggccttatta ttaagcaagt tggtctactt cttaactggc
     1081 cccttcttga aacaataggt gtctacgccc agcacttgac cagtggcgcc attggtgctg
     1141 gtgtagccta cagtatagga gctccccccc tagctatttt ttcttctatt attgcaggta
     1201 tccttggggg aggagctcta gttatggagg aaggagctct tattcttagt atcggtgaac
     1261 ctgtaggtgc ctttgtagca gcccttgcgg gagcggaaat gggtcgctgg gtaggcaaaa
     1321 aacaaaaact tgaaattatc cttgttcctt tttcaaccat tgttgtaggt ggcctggtag
     1381 gccattatgt tgctcctatt gtatcaggtt ttatgattgg ccttggaata tttataaatt
     1441 ttgctacaac ccaaaaaccc tttaccatgg gaattcttct ttcccttgtt atgggaatag
     1501 ctctgaccct gcctattagc agtgctgccc taggaattgg tttgggacta agcgggctgg
     1561 cagcaggggc ctcaacggta ggctgtgcct ctcatatgat tggctttgct gttgcctcct
     1621 atagggataa tggcttttct ggttttttat cccaagggct tggtacttcc atgctacaga
     1681 tgccaaatat aataaaaaaa cccatgattg ctctacctgc tatagttact tctattgtcc
     1741 tgggccctat ttccacctta gtttttaaga tgaagggaac tcccgttggt tcaggtatgg
     1801 gtacgagtgg ttttgtcggt caatttgcca cccttgaagc catggggcct tcttcggctt
     1861 tacccatctt cctaatgcac tttttgcttc ctggtctact ttctctagta gttagtgaat
     1921 ggatgagaaa aaaaggcctc attaaggccg gagatatgaa gcttactctc tagacgcctt
     1981 ctccatggcc tcccctttgg catagctagc ttgcatagcc ttttgaatgg ctctggctcc
     2041 cccccaatgt tcaagggcaa ggatgccttc aattgtcgtc ccccctgggg aggttacctg
     2101 gtctttaagc tctgcaggat gcaggcctgt ttctaacaaa agagaggcac tccccttcat
     2161 aatttgccca gccaggatat aggcttgatt tcttgggata ccccaagcta cagcctgatc
     2221 ggccatggct tcaatcaagt tcatcataaa ggccggcata actccacaaa ggctgccaaa
     2281 ggcctccatt agagactcat ccatatcatg gcaccagcca aagctctgaa agatttctct
     2341 tattaggtct ttttcttctt cctctagggc ttgactaaag caaagcccac taactgacat
     2401 tccaatttga gctggtgtgg cgggcatgga acgaactatt tttgtttttt ttgtaaaata
     2461 gctttgcatt ttctccaggc taaggcccgg tgccatacta acaataattt gattcttagt
     2521 aagaagtgtg gctagctctt gtagaaaaaa cggataatct ttgggcttta tagccaaaaa
     2581 aataatatca ctttgataaa tctcttctaa gctagagggg ccaaacccca acttctcttc
     2641 cagtttctgg gctgactctt tgctttttac tgtgtagatt agcttatctt ctcgcttact
     2701 tctaattccc tttaacatag ctagacccat attgcctaga ccaataaatc caattgtcat
     2761 ttcagtctcc tttttgaaca tgttatgata agtttaccac gagctgcagg aggaaatcaa
     2821 agtagattgt tttccttgaa tatctcagct ataggggtat aatagaacag aatagaatta
     2881 caggaggaaa aaatatgaat gttcttgtta ttaactgtgg atcgtcttcc ctaaaatttc
     2941 aacttttaaa tatggaaaac gaatccttac tagccagtgg gcttgttgag cgcatcggaa
     3001 tttccggttc tcaaatgatt cttaaaacaa gggatgaaga agatcatgtc gtaaaagtac
     3061 caatgaaaga tcattccatt gctttaaaaa tagttttaga tgctcttgtt gacccaagct
     3121 atgaaatcat caaagatcta aaagaaatta atgctgtagg ccaccgcgtt gttcacgctg
     3181 gagaattctt ttccaaatca gttattatcg ataaggaagt tatccaggcc attgaggact
     3241 gtgtagaact tgctccccta cataaccccc ccaacctact tggaatcaat gccatcttag
     3301 agcttatgcc tggggttccc aatgtagcgg tatttgatac agccttccat caaactatgc
     3361 cccctgaggc ctttatctat cccctgccct acgagtggta taaggacctt aaaattcgcc
     3421 gctatggttt tcacggcaca agccataagt tcgtagccga aaaagccgcc gactttttag
     3481 gaaaagacat ccagtcgcta aaaatcatta cctgtcatct aggtaacggt tcaagcatca
     3541 ctgccgttga aaacggaaag agtgttgata cttccatggg ctttacccca ctagaaggtc
     3601 tggtaatggg aactcgcgta ggttttctag atccagctat tatatcctat ctagagaaga
     3661 aaacagacta taccttggat gaaattactc atatcttaaa ccacaactgt ggagttcttg
     3721 gaatcagctg cctatctagt gactttaggg acctagggga tgctgctgct actggcaatg
     3781 agcaagctca gctggccctg gatattttcc gtcgtacggt taaaaagaaa attgctgcct
     3841 atgctgctgt tatgggtggg gttgacgcag ttgtatttac cggcggtatt ggagaaaacg
     3901 gcatcgaaga tcgagctggc attctcagcg gactagaatt tatgggcctt tatctagacg
     3961 aagaaaataa caaggtgcgc ggcgagacaa aaagtatctc tacaaacgat tctcccgtat
     4021 ctatcctggt tatcccaacc aatgaggaac tggcgatagc taaggataca aaactccttg
     4081 tagaggccta agcatcagga aactgatgct tttttttatc atgaaacttt taattataat
     4141 agctgaatac aacccctttc accagggcca taaataccat ttaagcaagg ccaagaaaga
     4201 aacgggagcc agccatagtc ttgttttaat gggaggaaac tttcttcagc gtggggatgt
     4261 ggctattgaa gataagttta tccgggcaaa aaaagccctg gatggtggag cagatcttgt
     4321 ggcagaactg ccctttgttt atgccagttc ttgggccaga gaattcgccc agggcggtgt
     4381 agccatcgcc aacaaaatcc ccttgcccgc caccctgagt tttggcagcg agacaggatc
     4441 catttcccaa ctaaaaaacc ttcttgcaaa aatcgatcag atagaaaaag ataaagagca
     4501 cagttatggt gatcttgtaa gaaaaaacct aatctgcccc ccaaagggag ccaatgatat
     4561 tttgggcctt gagtatttgc gggccctaaa ggacacaaaa agtattcata aggcccacac
     4621 cgtccttcgt ctaggccagg cctatcacga tgataaacaa agtacatttc catcggccag
     4681 ctccctaaga aggcaaataa aagaaggaga aatcccccat gataatcccc tttttcttga
     4741 ggattttagc gactatatct atagcgccct tcttaccaag gaccttctgc caacctatgg
     4801 catgatagag ggcctggaaa atcgtcttct taaggaactt gggccaaata taagcctgga
     4861 agaatatatc gaccgactct caaccaagcg ctatcgcccg gcaagactca gacgacttct
     4921 tatccaccac cttataggtt atacaaaatt agaccataaa aagcttaagg atgtggccta
     4981 tcttaggcct ctagcctata caaaaaaagg aacacagatt ctaagtatgg taaaaaacac
     5041 cccccttaag atcattcttc cccatgaaag acaaggtgat cctcttataa gtagatccct
     5101 ggaacttgat ctacttgctt caagggtcta taattttaag gcaggtttat ctcccgggga
     5161 ctttaagaga aatcttctgc atagataaaa aggaagctca ggcttccttt agttgttttc
     5221 aacagtaatt accccgctat aggtattcat tataaaggtt ttttctaata gggccccctc
     5281 tatatcctgc tgggctttta ttacaattt
//
LOCUS       DASRNA010000237         3976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_884385,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000237 DASRNA010000000
VERSION     DASRNA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884385"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="VFD08_06465"
     CDS             <1..1818
                     /locus_tag="VFD08_06465"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HZK10697.1"
                     /translation="ENPLETIPVRQALDRVNSKAIFAELSSPSFHSCAMDGIMLQSKT
                     SLAAREHRPLLVKKKDYLACDTGDPLIAPFDSVIMAEDLVEKEEGVLIFKPSHPWEHI
                     RSMGEDIIEKDLLFTAEHIFSPVDLSVLLAAGIKEVEVYKKPEIAIIPTGDELVSGEK
                     PLQGGQIIESNTAMLEALAQKSGALVKRFPVIKDNKEALLSAVKKALASDIVVMIAGS
                     SAGRDDLTSEIAQELGELLVHGIALRPGKPAVVASLEGKPFIGLPGYPVAAHIVFQEL
                     VVPLIRKKAHLPQEEKRKIKGSLTRRVVSSLSNKEYVRVQVGRVAGSYRITPTERGAG
                     ASLSLARSDGYLIVARNSEGFLRGEEVEVVLHNRLKNIDKRLVVTGSHDMVLDLINDL
                     FAQEGKESVLISSHVGSLAGLQNLKDKECHLAPSHLLGGDGRYNYEAMDLFFKTEKMA
                     MIHLVGRRQGIIVPKGNPKNIKGIKDLAGKTMINRQRGAGTRVLFDYLRKKENILAED
                     LPGYEKEATTHLAVALAVSQGDVDFGIGIEAAARTMGCDFIYLTDEAYELILYKESLA
                     SPEIQELIELTRSEGFKKRVKELGGYSTKMSGSIKFYEG"
     gene            1808..2755
                     /gene="moaA"
                     /locus_tag="VFD08_06470"
     CDS             1808..2755
                     /gene="moaA"
                     /locus_tag="VFD08_06470"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZK10698.1"
                     /translation="MKDSFGREINYLRLSLTDFCNFRCAYCMPKEGVEAKKRQEILTL
                     EEIYAIARHFVQMGVSKLRLTGGEPMLRPGHLGLIFSLSQLESLKDLAMTTNGSFLSS
                     QAQKLKEAGLKRVNISLDSLKADVFKEITHSDLGPVLEGIDAALECGLKVKINTVLLK
                     GINEGEIEDFARLTLDRPLDVRFIELMPLGERADFSKKHFLSCDRVLDKLELVKETEQ
                     IKGSAATLYRYKNGLGKIGLISPLSCNFCGECNRLRLSCEGKLRPCLHSDYYVDLLAP
                     LRRGEDLTPYILEALENKPLHHFLKEGHKTKESMHRIGG"
     gene            2757..3221
                     /gene="moaC"
                     /locus_tag="VFD08_06475"
     CDS             2757..3221
                     /gene="moaC"
                     /locus_tag="VFD08_06475"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004839396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZK10699.1"
                     /translation="MFSHMDKNLRGHMVDISEKDISLRQARAGGSIKMQKKTLEALAQ
                     LQVEKGDVLAVAQIAAIQGAKKTPDFIPLAHPILLQGVAVNFEYEEEGIYCEVTVKSQ
                     GKTGVEMEALTACSAALLTIYDMCKALDKTMEITGISLLEKTGGRSAYYEKK"
     gene            3208..3699
                     /locus_tag="VFD08_06480"
     CDS             3208..3699
                     /locus_tag="VFD08_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HZK10700.1"
                     /translation="MKKNEFTYGVVIASDSRSQDQRKDLCIDVVKKTLGEGFFCRYEN
                     IISDDKKKISKELLGLCDRGLDLIITSGGTGFSPRDNTPEATSEVIQRPTPGISEAIR
                     LISLEKTPFGMLSRGVSGIRGKTLIINLPGSPKAVRECLEGIRDPLIHGLEILLGLMG
                     EHE"
     gene            3714..>3976
                     /locus_tag="VFD08_06485"
     CDS             3714..>3976
                     /locus_tag="VFD08_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZK10701.1"
                     /translation="MNYERNRPLISRKNQKNLGKKKIAIVGLGGLGGYLAHGALRLGI
                     RRMLLFDGDVFDRTNMNRQIFCTEESLGKYKVDVVKKELLAIDP"
BASE COUNT         1210 a          734 c          989 g         1043 t
ORIGIN      
        1 gaaaaccccc ttgaaacaat cccggttagg caggcccttg atagggtcaa ttccaaggcg
       61 atatttgcag aactgtcttc tccttctttt catagctgtg ctatggatgg cattatgctc
      121 cagagcaaga caagtcttgc agccagagag catcggccct tgttggtaaa aaagaaggac
      181 tatcttgcct gcgatacggg agatccacta attgctccct ttgatagtgt tattatggcc
      241 gaagatctag tggaaaaaga agaaggggtc cttattttta aaccctccca tccttgggag
      301 catatacgct ccatgggaga agatattatt gaaaaggacc ttctatttac ggcagaacat
      361 attttttccc ctgtggattt aagtgttctt ttagctgcag ggataaagga agtagaagta
      421 tataaaaagc ccgaaatagc aatcatcccc acaggggatg agcttgttag tggagaaaaa
      481 ccgctgcaag gagggcaaat cattgaatct aatactgcca tgctagaagc tcttgctcaa
      541 aaatcagggg ccctagtaaa acgttttccc gttattaagg acaataaaga agcactttta
      601 tcggcggtga aaaaagccct tgcatccgat atagtggtta tgatcgctgg cagcagtgct
      661 ggcagggatg accttacttc tgagattgcc caagagctgg gggaactgct tgttcatggg
      721 atcgctctta ggccaggaaa acctgctgtt gttgcaagtc tagagggcaa accttttatt
      781 ggactgccag gatatccagt ggcagcgcat attgtttttc aagagcttgt cgtcccacta
      841 attcgaaaaa aagcgcattt gccccaagaa gaaaaacgga agattaaggg aagtctaaca
      901 agacgtgttg tttcctccct ctccaataaa gagtatgtaa gagttcaagt aggacgagtg
      961 gctggctctt atcgaattac ccccacggaa agaggagcag gagcttccct gtccttagca
     1021 agaagcgatg gctacctgat tgtggctaga aactcagaag gttttcttcg aggagaagag
     1081 gtagaggtag ttttgcataa tagactaaaa aacatcgata agcgacttgt tgttaccggt
     1141 tctcacgata tggttttaga tttgattaat gatctttttg cccaggaggg caaagaaagt
     1201 gttcttataa gttcccatgt aggttccttg gcaggtcttc aaaatttaaa ggacaaggaa
     1261 tgtcacttgg ccccctctca tcttttgggt ggggatgggc ggtataacta tgaggccatg
     1321 gatctctttt tcaagacaga aaaaatggcc atgattcatc ttgtaggccg tcgccagggc
     1381 attattgtgc ccaagggaaa tccaaaaaat ataaagggga taaaggacct ggcgggtaaa
     1441 acaatgatca accgccaaag aggggccggt acaagagtgc tctttgatta tctcagaaag
     1501 aaagagaaca ttttagcaga agaccttcct ggttatgaaa aggaagccac tacccatcta
     1561 gctgttgcct tagccgtcag tcagggggat gtggattttg gcattggaat tgaagcagca
     1621 gcaagaacaa tgggatgtga ctttatttat cttacagatg aagcctacga acttattctt
     1681 tataaagaga gtctagctag ccctgagatt caagagctga ttgagcttac aagatcagaa
     1741 ggctttaaga agagggtcaa ggaactagga ggatatagta ctaagatgag tggaagcata
     1801 aagttttatg aaggatagtt ttggtagaga aattaattat ttgcgtcttt cgctaacgga
     1861 tttttgtaat ttccgctgtg cctattgtat gcccaaagag ggggtagagg cgaaaaaaag
     1921 gcaggaaatc cttaccctag aggaaatcta tgcgatcgcc cgccattttg tgcagatggg
     1981 ggtgagcaag ctccgcctga cagggggtga gcccatgctg cgccctggtc atttgggtct
     2041 tattttttca cttagccaac tggaaagtct taaagatctt gccatgacca ccaatgggtc
     2101 ttttttatct agccaggcgc aaaaattaaa agaagcaggc cttaagaggg tcaatatatc
     2161 tcttgatagc ctaaaagctg atgtctttaa agaaataacc cattcagacc tggggccggt
     2221 cctggaggga attgatgctg cccttgagtg tggcctaaag gtaaagatta atactgttct
     2281 actaaagggc ataaatgagg gggaaattga ggattttgcc aggcttaccc ttgatcgccc
     2341 cctagatgta cgctttattg aacttatgcc cctgggagaa agggctgatt ttagtaaaaa
     2401 acactttctt tcctgtgata gggttttgga taaattagaa ctagtaaaag agacggagca
     2461 aattaagggt tctgcggcta ctctttaccg atataaaaat ggcctaggaa aaattggact
     2521 gataagccct ctttcttgta acttttgcgg ggaatgcaat cgactgcgtc ttagttgtga
     2581 gggaaaactt agaccctgtc ttcatagtga ttattatgtg gatttattgg cccccctacg
     2641 caggggagaa gatttaaccc cttatatctt ggaggccttg gaaaataaac ccttgcatca
     2701 tttcttaaag gaaggccata agacaaagga atcgatgcat cgtattggag gttagtatgt
     2761 ttagtcatat ggataaaaac ttacgaggac atatggtgga tattagtgaa aaagatataa
     2821 gccttcgcca agccagggca ggaggaagca taaaaatgca aaaaaagacc ctagaagctt
     2881 tagcccagct acaagtagaa aagggtgatg ttttagcagt tgcacaaatt gcagccatac
     2941 aaggagcaaa aaaaactccg gattttatcc ccctggccca tcccattctt ttgcaagggg
     3001 tagccgtaaa ttttgaatat gaagaagagg gaatttactg cgaggtcaca gtaaaatctc
     3061 aaggaaaaac cggggtggaa atggaagctc ttacggcttg ctctgcagca ctccttacga
     3121 tttatgatat gtgcaaggcc ctggataaaa ccatggagat cacaggaatt agtcttttgg
     3181 aaaaaacagg agggaggtca gcttattatg aaaaaaaatg aatttacata tggggtggtg
     3241 attgctagcg acagccgttc tcaggaccaa agaaaagatt tatgtatcga tgtagtgaaa
     3301 aaaaccctgg gagaaggttt tttttgccgc tatgaaaata taatcagcga cgataaaaag
     3361 aaaatcagca aggaactgct tggtctttgt gataggggac tagaccttat tattacttct
     3421 gggggaacgg gattttctcc tcgggacaat acaccagagg ctactagtga ggtcatccag
     3481 agaccaaccc cgggaatttc tgaagccatc cgccttattt ctttggaaaa aactcccttt
     3541 ggtatgcttt ctagaggggt gagtggaata cgcgggaaaa ctttgataat caatctgcct
     3601 ggaagtccta aggctgttag agagtgtcta gaaggaatta gagatcccct gattcacggc
     3661 ctagagattc ttttagggct gatgggagaa catgaataaa aagaggaaac aggatgaatt
     3721 atgagagaaa taggcccctg attagtcgaa aaaatcaaaa aaatctagga aaaaagaaaa
     3781 tcgccattgt aggcctggga ggccttggcg gatatctggc ccatggagcc ctacgcctgg
     3841 gtattagaag gatgctttta tttgatgggg acgtttttga taggaccaat atgaatcggc
     3901 aaattttttg cacggaagaa agcctgggaa aatataaggt ggatgtggta aaaaaagaac
     3961 tacttgccat tgatcc
//
LOCUS       DASRNA010000238         4984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_517425,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000238 DASRNA010000000
VERSION     DASRNA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517425"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1337
                     /locus_tag="VFD08_06490"
     CDS             192..1337
                     /locus_tag="VFD08_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZK10702.1"
                     /translation="MEFGLSNEHLMLKKLFEEFAVNQVEPLAADIDENESFPRETVDK
                     MAEMGMLGIPFPKEYGGTGGDNLAYAMAVEELSKKCATTGVIVSAHTSLGGAPIHEYG
                     TAEQKEKFLVPLAKGEKLGAFGLTEAGAGTDAGGQETTAVLDEEKGEYILNGTKIFIT
                     NAGEAQIYIIFAMTDKSQGTRGISAFIVESDTPGFSVGPPEKKLGIRGSATAELIFED
                     CRIPQENLLGKIGMGFKIAMKTLDGGRIGIAAQALGIAQGALDETVQYVKERKQFGRP
                     LSKFQNTQFVLADLENRIQAARLLVYRAAWNKDNGLPYSVAAANAKLFASEAAMDVTT
                     KCVQLFGGYGYTRDYPIERMMRDAKITEIYEGTSEVQRMVISSALLR"
     gene            1347..2132
                     /locus_tag="VFD08_06495"
     CDS             1347..2132
                     /locus_tag="VFD08_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZK10703.1"
                     /translation="MKIIVCIKQVPDTTEVRLDPKTGTLIREGVPSIINPDDLAGLEE
                     ALKIKEETGAKVTVLSMGPPQAAEALREAYAMGADEVILLTDRAFAGADTWATSSALA
                     GAIRTMDYDLIIAGRQAIDGDTAQVGPQVAEHLVIPSISYVEEIKEINDDHMIVKRQF
                     EDRYQLIKVNFPCLITTLGGMNDPRYMRISRVYDAYREDVVITKTAADIEVDPANLGL
                     KGSPTSVRKSFTKGAKSAGKVFDVEAREGAKIIVDALLERFII"
     gene            2142..3155
                     /locus_tag="VFD08_06500"
     CDS             2142..3155
                     /locus_tag="VFD08_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZK10704.1"
                     /translation="MKDYKGVMVFVEQRDGHIQNVSLELIGKAKELAKDLQTDVSAVI
                     VGKEVSALADDLAHYGADHIYAVEDDRLEKYMTEPYVKAVVEVISTKKPEVVLFGASS
                     IGRDLAPRVSARVHTGLTADCTKLDIDEETGLLLQTRPAFGGNLMATIVCKDYRPQMS
                     TVRPGVMQKVEPDMTLEADLEVFDLSFEAKDFNVEILSEEVETKKKIKIEDADILVSC
                     GRGVGGPENLPLLEDLAKVIGGEVSGSRATIDAGWLPKDHQVGQTGKTVRPGVYFACG
                     ISGAIQHLAGMEESDFIVAINKNPDAPIFNTADLGIVGDLHRVIPEFVRLLEVEKAKK
                     ANL"
     gene            3264..4019
                     /locus_tag="VFD08_06505"
     CDS             3264..4019
                     /locus_tag="VFD08_06505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK10705.1"
                     /translation="MKQLPPLPPFAAYIFSILVLFIFLIFVFILQRKLSYNRKKYPGL
                     LLPVFLFLGSILRSYALYQQDDVFEAVLTFLLVNLLTLYSIKQYVEERRKRKMKTKGI
                     EFAPVKTHKDLHPLYKEEGLEIGNEPVETRFVRSLGYKKGRGKKGKYLAGASLGKRGD
                     YLILDSIAVDKKLQGQGIGERLMNELLHDLPYGEDLYLIAKAHDFFEKLGFEDIPIEE
                     CPPVFGCLNCDRLDVDCFPKPMVLRKGKTLRTD"
     gene            4162..4896
                     /locus_tag="VFD08_06510"
     CDS             4162..4896
                     /locus_tag="VFD08_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZK10706.1"
                     /translation="MSIFAWYGYEGSLYKRAKTLTDIGFSSLSLWWEREDGKSAEENV
                     KVLRDMGLNIDLVHLPYFPGGFWGKEKLPFEEAFLRALNELHSGEIARAVFHPTSIKE
                     LDLAIDQEGLELFYRLYQRAKILGVDLLIENLREDPHLFTLLENFDLGLCLDTGHLGI
                     SGNEKRLKKYFPRVRALHLHDNDGKSDLHLIPGDGILPLNTWLKDLPKGVEYHLEINR
                     GLSEVYKDYNEVEYLTRAKKSLSLLE"
BASE COUNT         1550 a          865 c         1116 g         1453 t
ORIGIN      
        1 aagcctgtaa acaaatgagt ttaatgtaaa aagggggccg agtattgcgt aaaaatgctt
       61 atttgcacta cattttaatt attatttcat tatcgcttga atttttcttg agaagtgata
      121 aacttttaac tagaaggacc actataaagg ctttgcctac tataagggca tgcccaaaat
      181 aaggaggaat cgtggagttt ggactatcaa atgagcatct tatgctcaaa aaattgtttg
      241 aagagttcgc tgtaaatcaa gtcgaaccct tagctgcaga tattgatgaa aatgagagct
      301 ttccccgaga aaccgtagac aaaatggcag aaatgggtat gcttggcatt ccttttccca
      361 aagaatatgg cggaactggt ggagacaacc tagcctatgc tatggctgtt gaggaactct
      421 caaagaaatg tgcaactaca ggcgttattg tttctgccca tacatcttta ggtggagcac
      481 ccatccatga atatggaaca gcagaacaaa aagaaaagtt tttagtacct ttagctaaag
      541 gggaaaaact tggtgctttt ggtttaactg aagctggtgc aggaacagat gctggagggc
      601 aagagactac agcggtttta gatgaagaaa aaggtgagta tatactcaat ggtaccaaga
      661 tttttatcac caatgctggc gaagctcaaa tttatataat tttcgctatg acagataaga
      721 gtcaaggaac aagaggtatt tccgcattta tcgttgaaag tgatactcct ggattttctg
      781 taggacctcc agagaaaaaa ttagggattc gtggttctgc tacagcagaa cttatttttg
      841 aagactgccg tattccacaa gaaaacttat tagggaaaat aggcatgggc tttaaaattg
      901 ccatgaaaac attagatggt ggtcgtattg gaattgctgc tcaagctcta gggattgccc
      961 agggagctct tgatgaaaca gttcagtacg ttaaagaaag aaaacagttc ggtcgtccac
     1021 tttcaaaatt ccaaaacaca caatttgtat tggccgatct tgaaaaccgt attcaagctg
     1081 cacgacttct tgtatatcgc gctgcttgga ataaagacaa tggcctcccc tattctgtgg
     1141 ctgctgctaa tgctaagctt tttgcttctg aggcagccat ggacgttact accaaatgcg
     1201 tccagctctt tgggggctac ggttatacca gagattatcc cattgagcgc atgatgcgcg
     1261 atgcaaaaat cactgagatc tacgaaggta ccagtgaagt acaacgcatg gtcatcagta
     1321 gtgctctgtt gcgctaggag gttaggatga aaattattgt ctgtattaaa caagttccgg
     1381 acacgacgga agtgagatta gatcccaaaa cagggacttt aattcgtgaa ggtgttccta
     1441 gcattatcaa tcccgatgac cttgctgggc ttgaggaagc gttaaaaatt aaagaagaaa
     1501 ctggggcaaa agtcacagtt ttaagtatgg gtccccccca agcagcagaa gctcttcgtg
     1561 aagcctatgc tatgggtgca gatgaagtaa ttttgcttac agaccgcgcc tttgctggag
     1621 cggatacctg ggcaacatct tctgctttag ccggagccat tcgcactatg gactatgatc
     1681 ttattattgc aggacgtcaa gctattgatg gagatacagc gcaagttgga ccacaagttg
     1741 ccgagcattt agtgattccc agcattagtt atgttgaaga aatcaaagaa attaatgacg
     1801 atcatatgat tgtaaaacgt caattcgaag atcgttatca attaattaaa gtgaatttcc
     1861 cttgcttaat cacgactctt ggtggaatga atgatccacg ctatatgcgt atttcacgag
     1921 tttatgatgc ctatagagaa gatgttgtaa tcactaaaac tgcagcggat attgaggttg
     1981 acccagccaa tcttggtctt aagggttcac ctacaagcgt gcgtaagagc tttacaaaag
     2041 gcgcgaaatc tgctggaaaa gtttttgacg ttgaagccag agaaggagca aagattattg
     2101 ttgatgctct tcttgagcgc tttatcatat aggaggattt catgaaagat tataaaggtg
     2161 taatggtttt tgttgagcaa agagatggac atatccaaaa tgtctcctta gagctcattg
     2221 gaaaagccaa ggaacttgct aaggacctcc agactgatgt aagtgctgtg attgtaggta
     2281 aggaagtaag tgctcttgct gatgacctag ctcattatgg agctgatcat atttatgctg
     2341 tggaagacga tcgccttgaa aaatatatga cagaacctta tgtaaaggca gttgttgaag
     2401 taatcagcac aaagaagcca gaagttgtac tttttggcgc tagttcaatt ggaagagacc
     2461 tagctcctag agtttctgct agagttcaca caggactaac tgcggactgt acaaaacttg
     2521 atattgacga ggagacaggt cttctacttc agactcgtcc tgcctttggt ggtaacctta
     2581 tggctactat tgtatgtaag gattatcgtc ctcaaatgtc aacagtacgc cctggggtta
     2641 tgcaaaaagt cgaaccagat atgactttag aggcggatct tgaggttttt gatcttagct
     2701 ttgaagcaaa ggacttcaat gttgaaattc tttcagaaga agttgagaca aagaaaaaaa
     2761 tcaaaattga agatgccgat attcttgtat cctgtggacg tggcgtgggt ggacctgaaa
     2821 atctaccact tcttgaagat ttagcaaagg ttatcggagg cgaagtttcc ggatcaagag
     2881 ccacaatcga tgcaggttgg ttgccaaaag accatcaggt tggtcaaaca ggtaaaacag
     2941 tacgccctgg tgtatacttt gcttgtggta tttctggagc tatccagcat cttgctggta
     3001 tggaagaatc agattttatt gtagctatca ataaaaaccc tgatgctcct atattcaata
     3061 cagctgattt aggtattgtt ggtgatcttc atagggttat tcctgagttt gttagacttt
     3121 tagaagttga aaaggctaaa aaagcgaatc tataataaaa ataagcggta tattctaaat
     3181 ataccgtttt ttttaccttt tttttcaaat ataatgattt agtccgggca atcatgtata
     3241 ctttaagtag ataggagtat gccatgaaac aattaccacc acttccaccc tttgctgcat
     3301 atatttttag tatcttggtt ttatttattt ttcttatttt tgtttttata cttcaaagaa
     3361 aattgtccta taatagaaag aaatatcctg gactcttact gccggtattt ctctttttgg
     3421 gctctatctt gcgctcttat gcattatacc aacaagatga tgtttttgaa gcggttctga
     3481 cttttctact agtgaatcta cttactcttt attcaatcaa gcagtatgta gaagaaagaa
     3541 ggaagagaaa gatgaaaacc aaaggaatag aatttgcacc tgttaagacc cataaagacc
     3601 ttcaccccct ttacaaagaa gaaggcttgg aaataggaaa tgaaccggta gaaactcgct
     3661 ttgtacgtag tttgggctat aaaaaaggaa gaggaaaaaa ggggaaatat cttgctggag
     3721 cctccctagg aaaaaggggt gactatctta ttcttgatag tatagctgtg gataaaaagt
     3781 tgcaaggcca aggcatagga gaaagactga tgaatgagct tcttcacgat ttaccctatg
     3841 gggaagattt atatcttata gcaaaggccc atgatttttt tgaaaaactg ggttttgaag
     3901 acattcccat tgaagagtgt cctcctgttt ttgggtgttt aaattgcgat cgactggatg
     3961 ttgactgctt tcctaagcct atggttttaa gaaaaggaaa aactcttagg actgactagc
     4021 agccatatgt gcctcctaga aaaaaggggg cttttttatt caaagaagcc cttcttataa
     4081 agcaagagaa agaagcctgc ccttttatga aaaaacattt atctaaataa aaaatagagt
     4141 atactgtatc taggaggggc gatgtctatc tttgcgtggt atgggtatga aggaagttta
     4201 tataaaaggg caaaaacact tacagatata ggtttttctt ccctcagcct ttggtgggaa
     4261 agagaagatg gcaagtctgc agaagaaaac gtaaaagtct tacgagatat ggggctaaat
     4321 attgacctag tacacttacc gtattttccc ggtggctttt ggggaaagga aaagttgcct
     4381 tttgaggaag cctttttacg ggcccttaat gaactccact caggagaaat cgctagagct
     4441 gtctttcatc ctaccagcat aaaggagcta gacctagcta tagatcaaga gggtcttgag
     4501 cttttttata ggctttatca aagggctaag attttgggag tcgatctgct aatagagaat
     4561 ttgcgagaag accctcatct ttttaccttg cttgaaaatt ttgatttggg tctttgtctt
     4621 gatacaggcc atcttggcat atcaggaaat gagaagaggc ttaagaaata ttttcccaga
     4681 gtgagggctc tacacctgca tgataatgat ggtaagagcg atcttcacct aatacccggt
     4741 gatggaatac taccccttaa tacttggctt aaggatcttc caaagggagt agagtatcat
     4801 cttgaaatca accgaggact tagtgaggtt tataaagact ataatgaagt tgaatatctt
     4861 accagggcta aaaagagttt gtctcttttg gagtaatcaa tgaaaaaaga ttctgaaaac
     4921 aaaaaaaaga agagccccca agaaagggac atccttcttt taaaaacagt atataccatt
     4981 attg
//
LOCUS       DASRNA010000239        13929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_888251,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000239 DASRNA010000000
VERSION     DASRNA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13929
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888251"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1082)
                     /gene="ispG"
                     /locus_tag="VFD08_06515"
                     /gene_synonym="gcpE"
     CDS             complement(45..1082)
                     /gene="ispG"
                     /locus_tag="VFD08_06515"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZK10707.1"
                     /translation="MTKAIKIGSRIIGGGFPLAIQSMTTTKTEDIQATVKQILELEDL
                     GCEIIRVTANSREAALAIKEIKKQINIPLVADIHFDAEMALLALESGADKIRINPGNM
                     PLKALDEILALAQKKEAAIRIGINSGSLEKEFLDQYGVSVEAMLESMKKYVSYFESKA
                     FENIVLSAKASDVALTVAVNEELGKLFTYPLHLGVTEAGIYETAVIKSAIGIGSLLLK
                     GYGDTIRVSITGDPKQEIPVAKNILKVLGLRSGINIISCPTCGRCQMDLDDLASRVSE
                     ATRNMDKDLTIAIMGCAVNGPGEARAADIGIAGGKKSGLLFKKGEIIEKVDEKDLFNR
                     LMEEIKGAHDD"
     gene            complement(1082..2080)
                     /gene="rseP"
                     /locus_tag="VFD08_06520"
     CDS             complement(1082..2080)
                     /gene="rseP"
                     /locus_tag="VFD08_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZK10708.1"
                     /translation="MSTAINFIIIFNLVVFFHEMGHFLMARKHGVTVHEFALGMGPVL
                     FSKTKGLTQYSLRVLPLGGFCKMEGEDDSSESSGSFSQRTAWQRFSIILAGPIMNFVL
                     AFLLFLGLNGFMGVPSLSISEVLENSPADLVGIEAGDQIVEVNGVDIKSWDDMQVSIG
                     ESVGDIEVKVQKPSKTIESFLISPEIHENTRIIGIVQGRDKDFLLAIKYSLSQMALIF
                     KSFFVFFSQLFKGGLTTNDVAGPIGIVKLVDDVTKAGLFTVIFFTAFFSINLGVMNLL
                     PIPALDGGRLLFIIIELVRGKPLDPDKEGFVHMLGFVFLMLLMLFITFLDIKKWIG"
     gene            complement(2065..3204)
                     /gene="dxr"
                     /locus_tag="VFD08_06525"
     CDS             complement(2065..3204)
                     /gene="dxr"
                     /locus_tag="VFD08_06525"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZK10709.1"
                     /translation="MKKIGLLGASGSIGKQTLDIIRRERNFKLSFFSVHRNIDKAKKI
                     IAEFSPEYAIITCEDAYKNLGSFYDSTKIYGPSELEYILKNKEVDIVVNAMMGMSGLS
                     SSWWILNGGGKLALANKESLVSAGKLLVNLMEKEGGEILPVDSEHSALWQCMEGNNKR
                     GIEKLILTASGGAFRDLTKNEMLNKNAKEALKHPNWSMGEKITIDSATMMNKGLEVIE
                     AHWLFGVAQENIDVLIHRESVVHSLVQYKDGSLLAQLGISDMRQPIHYALHEKERMPL
                     DLARLDLSQISQLHFEAVDKEKYPTLEYCYRALKEGGNRPLLLNTINEVLVEHFLNDK
                     ISLKDIFSGLYRSFDEAFFEPRDLQDLLDEEKRIRTKTEELCLQQ"
     gene            complement(3385..4146)
                     /locus_tag="VFD08_06530"
     CDS             complement(3385..4146)
                     /locus_tag="VFD08_06530"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZK10710.1"
                     /translation="MNKRVISAIVGISLLFILLYIGGLPLRIGLIMLTLLCMMELKGT
                     LGSSFRLDDFISAIFVVLSFSLPWDLALPLGFLYIVLSLGLSLIMNPLDLKRVYLNSF
                     AYFFTFLPFLLLYRLIAFSPIKYLYTLALVLAWGNDTFAYYIGTYFGKTNYTKISPKK
                     TIEGALGGLLGGILLIALVKAVFIKDISFLTLIIFGLLGGIFSQMGDFFGSYIKRSTG
                     VKDFGVVMPGHGGMLDRFISVMFSTIVLYFMHNFF"
     gene            complement(4139..4825)
                     /gene="uppS"
                     /locus_tag="VFD08_06535"
     CDS             complement(4139..4825)
                     /gene="uppS"
                     /locus_tag="VFD08_06535"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZK10711.1"
                     /translation="MLPKHIGFIMDGNGRWAQSKGLSRSDGHRAGTENIRTIIKTSKD
                     LKIKYLSFYAFSTENFKRPLKEVNTLMGLLIEFFNRELPELHKEGACIHILGDLSLFS
                     APVRFVLEKAMQTTKNNDEIHICLALGYGGRAEIIRAVNLLLENNIKQVDEESFKEYL
                     YTAGMPDPDFIIRTSGEFRISNFLLFQLAYSELYFSPIYWPDFDKKQYLIALEEFSNR
                     KRRFGGIDNE"
     gene            complement(4825..5379)
                     /gene="frr"
                     /locus_tag="VFD08_06540"
     CDS             complement(4825..5379)
                     /gene="frr"
                     /locus_tag="VFD08_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZK10712.1"
                     /translation="MEDYNRNLQAQMNKALDFFKSELSNVRAGRAHPGILDRIVVNYY
                     GVDTPLNQLATITAPEARLLVISPYDKTSISNIEKAILTSDLSLNPNNDGKIVRLNLP
                     LLTEENRIKLSKLVKKLGEETKVTIRNERRSTIEQIKKEEKDSLIREDESIRAQEDVQ
                     KIVDKAVEEVDRLVDEKNNEIMAV"
     gene            complement(5384..6091)
                     /gene="pyrH"
                     /locus_tag="VFD08_06545"
     CDS             complement(5384..6091)
                     /gene="pyrH"
                     /locus_tag="VFD08_06545"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZK10713.1"
                     /translation="MKLKYKRILLKLSGEALAPASGTGIDDHVLAEIASQIAELYENG
                     YEISIVVGGGNFWRGRSSKEMDRSTADYMGMLATVINGLALQDSIEKLSIPTRVMSAI
                     EMPKVAEPYLRRKAVSHLEKNRIVIFSGGTGNPFFSTDTTAALRAAEIEAEIVLFAKT
                     IDGVYDKDPKFNADAKRYREMDYRDLLIGDLKVMDQTAATLLAENKIPCFVFAMNKQD
                     SLLRALESQEDGTIIRR"
     gene            complement(6194..7132)
                     /gene="tsf"
                     /locus_tag="VFD08_06550"
     CDS             complement(6194..7132)
                     /gene="tsf"
                     /locus_tag="VFD08_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZK10714.1"
                     /translation="MSFGTKEIKELRQRTGAGMLDCKKALEENNGDIEAAVEYLRKKG
                     IASSEKKSSRIAAEGLVAMVLDEDNAAGTLVEVNAETDFVAKNKDFSGFVETLAALVH
                     KQKPAEMEDLNSLRIEDKTVSEFLTELIARIGENMNIRRFKHLEVDSGSVFGYLHGNG
                     RIGALVGFKSNAAAADLAELGKDLAMQVASMSPKYISRNDVDANYIDSEKKILLEQAL
                     NENRQAEAAGKKPKPENIIEKMVEGRLNKELQEVCLLEQSFIKDSDLTVGALVKNIEK
                     TLGEKIEVVSMVRYEVGEGLEKRVENFAEEVAKQMA"
     gene            complement(7155..7940)
                     /gene="rpsB"
                     /locus_tag="VFD08_06555"
     CDS             complement(7155..7940)
                     /gene="rpsB"
                     /locus_tag="VFD08_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZK10715.1"
                     /translation="MSVVSMKSLLEAGVHFGHQTKRWNPKMKPYIYTERNGIYIIDLQ
                     QSVKMLEEACDFIRDLASKGGKVLYVGTKKQARIAIEQEAKRAGMYYINQSWKGGLLT
                     NFQTIRKRIDYLFELEEMEADGTFEALPKKEVILLRREKDKLERFFNGIRDMRELPDA
                     IFIIDQNKEKIAIHEAKLLGIPVVGILDTNCDPDEIDYPIPGNDDAIRAIKLFSATIS
                     TAILEGKAAMDAAEDEQMLEEQEVTQEANKEEEIEEDVSVDKE"
     gene            complement(8086..8328)
                     /locus_tag="VFD08_06560"
     CDS             complement(8086..8328)
                     /locus_tag="VFD08_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZK10716.1"
                     /translation="MEIKILGTGCTKCEKLEKNLEIALKELNIEARIENVEDLVELVS
                     YGVMSTPGFVINDEVKSVGKALSVKQIKKLLSKSAQ"
     gene            complement(8341..9345)
                     /locus_tag="VFD08_06565"
     CDS             complement(8341..9345)
                     /locus_tag="VFD08_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK10717.1"
                     /translation="MTILSFLNDQFLKMKWLWDVVEILVVKIFNLSMDTRVGGSIHFF
                     IYDVIKIFILLTVMIFFISYIQSYFPPERTKKLLGGIKGIRGNIIGALLGILTPFCSC
                     SSIPIFIGFVSAGLPLGATFSFLISSPMIDLAALMLLLSFFGIKIAFTYVAAGVGVAI
                     LGGIIIHKMQMENYVESYVWGVEAKEVKLDEMTRKERLDFSLEQVEDIIKRVWPYILI
                     GVGIGAGIHNWLPQSIVEKILGNQNPFSVLIAIIIGVPIYADIFGTIPIAEALISKGV
                     GIGTVISFMMAVTTMSIPSLVMLSKVVKAKLLASFVFVVVIGIIIIGYGLNALSPFLL
                     "
     gene            complement(9356..9706)
                     /locus_tag="VFD08_06570"
     CDS             complement(9356..9706)
                     /locus_tag="VFD08_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZK10718.1"
                     /translation="MQNLINYLKLLSDETRLRIMVLLYHDEFCVCQITGITGISQPNV
                     SKHLARLRDIGLVKDQRKEQYTFYSLNLKDTLFNKILSEIVQGIDEYPVLKLDLEKSK
                     AAAIYIELREANKA"
     gene            complement(9715..10647)
                     /locus_tag="VFD08_06575"
     CDS             complement(9715..10647)
                     /locus_tag="VFD08_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZK10719.1"
                     /translation="MSKKTKLYLITGFLGAGKTTFLTKVLEDLEGSKVAVIMNEFGKV
                     GIDGPIIQKEGMELLELNSGSIFCSCLQLSFVSALVEIANRDMDYLFVESSGLADPSN
                     IDEFLDAVMIAKGEVYDYSGALCIVDGVNFLDQIKDIETVQRQLKFAHLVIISKADLI
                     SEETLEQVKGKIREINKNVDLVESEKGIVNYSFLDKNLMEKNWIGSEETTNTKENRPK
                     TLTLTYEGSLTKEKMGQFLDLIKEDSYRIKGFFELEDGWNQIDVVGKRIDYKKTDQVW
                     PNSELVIISRIGVHIIRPIFKAWESVVGRKMRLK"
     gene            complement(10797..12857)
                     /gene="topA"
                     /locus_tag="VFD08_06580"
     CDS             complement(10797..12857)
                     /gene="topA"
                     /locus_tag="VFD08_06580"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZK10720.1"
                     /translation="MAKNLVIVESPTKAKTIQRYLGKNYKVMASVGHVRDLPKSQMGI
                     DIENNFKPKYITIRGKGPVLAELKKEKKKAQNVLIATDPDREGEAIAWHLATALKLDD
                     SEPIRVTFQEITKDAIKKAIKNPTHIDQRLVDSQQGRRILDRLVGYEISPILWRKVMK
                     GLSAGRVQSVTTRLIIQRERKIKAFKPEEYWVFTGKFLSEDKKAFSADLWRYKDKKID
                     VSSQEEAMRIKKEIESTNFSISSIEEKKRRRFPYSAYTTSTLQQDASVRLNFSSGKTM
                     MVAQKLYEGVRVGKSVEGLITYMRTDSSRISETAVSSCKDFIVENYGKKYSAPKKGGA
                     GKDAQDAHEAIRPTSVEHTPEKMASYLKRDELRLYTLIWQRFVASQMKQAQFEGLEIT
                     ISGGDYKFRTKGERLVFDGFLKVYDARNTKDIDLPPLKEGENTLLKKFKEEQKFTQPP
                     ARYTEASIIKELEENGIGRPSTYAPTISAVVKRGYVVKEKKSLKPTELGEITNEIMEE
                     NFLQIVEPEFTAKMEEELDKISNEEVSWQEVIGDFYDILQPMLEEAEKRIQKYDLNEL
                     TDIDCDKCGSKMLIKKTIKGSFYACSNYPECKNTKPILKKIGIACPLCENGQIVERKT
                     KKLRIFYGCDQFPECRFATWDKPVDRKCPKCDSILVEGKGRRKDTIHCSNKECDYVED
                     KKTE"
     gene            complement(12839..13489)
                     /gene="dprA"
                     /locus_tag="VFD08_06585"
     CDS             complement(12839..13489)
                     /gene="dprA"
                     /locus_tag="VFD08_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZK10721.1"
                     /translation="MIIEQEKFPRGLLFLHELPKLLYAQGNLDLLEKKCVTIVGSRKA
                     TKRGLAQTRKIVEKLVARDIVIVSGFAKGIDEMAHKSAFEFGGKSIAILGTGLNSLYP
                     RGRDLLRWRMEEEGLLLSEYPTNVGPRPHHFPRRNRLLAALSPITIIVEAEIKSGTMI
                     TAKEALGQGKDLWVVPGSPEDPLSLGPNYLIFQGANPLYHYDLIDHWEEELWQKIW"
     gene            complement(13540..>13929)
                     /locus_tag="VFD08_06590"
     CDS             complement(13540..>13929)
                     /locus_tag="VFD08_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZK10722.1"
                     /translation="GYGVGWADQKEIDKLNILNATKLAMQRAIKKLGFSLDALLLDGT
                     PMDLHPMEWALIGGDDLSNEIAAASILAKVTRDHWMMEMDKHFPVYGFAQHKGYGTAQ
                     HIEALREFGPCHLHRKSFLSFLNDNLK"
BASE COUNT         4114 a         2930 c         2211 g         4674 t
ORIGIN      
        1 gaattataat ttcaccaaat tctcttttaa gatattcctc aaaattaatc atcatgagct
       61 cctttaattt cttccattaa acgattaaaa agatcttttt cgtcgacttt ttcaataatc
      121 tccccttttt taaataaaag gccagatttt ttacctccag caataccgat atctgctgcc
      181 cttgcttccc ctggaccatt tacagcacag cccataatag caatggtaag gtccttatcc
      241 atattccttg tagcttcgct tacccttgag gccaaatcat ctaaatccat ttgacaccgg
      301 ccacaagttg ggcaactaat aatatttatt ccagagcgca aacccaggac ctttagaatg
      361 tttttggcaa cgggaatctc ttgtttagga tctcctgtta tagaaactct tattgtatca
      421 ccatacccct ttaataaaag actaccaatg cctatcgctg atttaatcac agctgtttca
      481 tagattccag cctcggtaac accaaggtgt agggggtagg taaaaagctt acctaactct
      541 tcatttacgg ctacagtaag agcaacatcg cttgccttag cagaaagaac gatattttca
      601 aaggctttgc tttcaaaata agaaacatat tttttcatgc tctccagcat tgcctcaacg
      661 cttaccccat actggtcaag aaattctttt tctagactcc ctgaattgat accaattcga
      721 atggcagcct cttttttttg agctagtgct aagatttcat ccaaagcctt tagtggcata
      781 tttccaggat tgattcgaat cttatctgct cctgattcta gtgcaagtag ggccatttca
      841 gcatcaaaat gtatgtccgc cacaaggggt atattgattt gtttttttat ttcttttatt
      901 gccagggcag cttctctact attagctgtc acacgaatga tctcacaacc taagtcctca
      961 agttctaaaa tctgcttgac agttgcctga atgtcctcag tcttagttgt agtcatagat
     1021 tgtattgcaa gtggaaatcc ccctccaata atacgactac ctattttaat tgcttttgtc
     1081 attatcctat ccatttctta atatctaaaa aggtaataaa caacataagg agcataagaa
     1141 aaacaaagcc caacatgtgt acaaagccct ctttatccgg atctaagggt tttccacgga
     1201 ctagttctat tataataaaa agaagtcttc ccccatcaag ggctggtata ggtagtaggt
     1261 tcataactcc aagattgatg ctgaaaaaag cagtgaaaaa gataacggta aaaagtcctg
     1321 ccttggtaac atcgtccact agttttacaa tcccgatggg tcccgctaca tcattggtag
     1381 ttagacctcc tttaaagagc tgcgaaaaga agacaaaaaa actcttgaaa attaaagcca
     1441 tttgacttag ggagtatttg atggcaagta gaaaatcttt atccctacct tgaacaattc
     1501 ctataatacg ggtgttttca tgaatttctg ggctaatcaa gaagctttct atagttttgc
     1561 ttggcttttg tactttaacc tcaatatctc caacactctc tcctatggac acttgcatat
     1621 catcccagct tttgatgtcc actccattaa cctcaacaat ttgatctcca gcttcaattc
     1681 caactaaatc agcaggactg ttttctaaga cttccgatat gctaaggctg ggaactccca
     1741 taaaaccatt caagccaaga aataaaagaa aggccaaaac aaagttcatt atgggacctg
     1801 caaggattat actaaagcgt tgccaggcag ttctttgact aaaactacca ctactttcac
     1861 tggagtcatc ttcgccctcc attttacaaa atccacccaa aggcagaact cttagagagt
     1921 attgggtaag gccttttgtt tttgaaaaca aaaccggacc catacctaag gcaaattcat
     1981 gaacggtaac cccgtgcttt ctagccataa gaaagtgtcc catttcatga aaaaacacaa
     2041 ctagattgaa tatgattata aagtttattg ctgtagacat aattcctccg ttttagtgcg
     2101 aattcttttt tcttcgtcaa gaagatcttg taaatcacga ggctcaaaga aagcttcatc
     2161 aaagcttcga tataatcctg agaatatatc ctttaagctt attttatcgt ttaaaaagtg
     2221 ttccactaaa acttcattta tggtatttag taaaagcggt ctattgccac cctcttttaa
     2281 ggctctgtag caatattcta gtgtggggta tttttcctta tcaactgctt caaagtgcaa
     2341 ttgtgatatt tggcttaggt caagtcgtgc taagtctagg ggcattcttt ctttttcatg
     2401 tagggcgtag tgaattggtt gacgcatatc agagataccc aattgagcta aaagactacc
     2461 gtccttatat tggaccaagg aatgaaccac actttcccgg tgaattaaaa catcaatgtt
     2521 ttcttgcgcg acaccaaaga gccaatgggc ttctattacc tccaggcctt tattcatcat
     2581 ggttgctgag tcaatagtaa ttttttctcc catgctccaa tttggatgtt taagggcttc
     2641 cttggcattt ttatttagca tttcattttt tgttagatca cgaaaagccc ccccagatgc
     2701 agtaagaatg agtttttcaa tccctctttt attatttcct tccatgcatt gccatagggc
     2761 actatgttca gagtccaccg gaagaatttc tccgccctct ttttccataa gatttacaag
     2821 tagtttacct gcactgacta aactctcctt gttggccagg gcaagtttgc cacccccatt
     2881 taaaatccac caactagagg atagaccact cattcccatc atggcattta ccacaatgtc
     2941 aacttcctta tttttaagaa tatactctaa ttcacttggc ccataaattt ttgtactatc
     3001 gtaaaaactc cctaaatttt tataggcatc ttcacatgtt attattgcat actccggaga
     3061 aaactcagca atgatttttt tggccttgtc aatatttcta tgaacagaaa agaaagaaag
     3121 tttgaaattt ctctctcttc gtattatatc taatgtttgc ttaccaatgg aaccactggc
     3181 tcctaataat ccgatttttt tcattatcac ctcgctaact tataagtata cactagaatg
     3241 agtatacttt ctagacttga aattttctta aaacagttct aactggctta gtcaggtctt
     3301 agttctattc aacaaccgtt cttgcaataa aaaaaggaga agtcttctcc ttttatctta
     3361 catcactaga ctctgattat gagcttaaaa aaagttatgc ataaaatata atacaattgt
     3421 tgagaacata acagaaataa atcggtccag cattccacca tgtcctggca ttacaacacc
     3481 aaaatcctta acaccagtac ttcttttaat ataactacca aagaaatctc ccatttgaga
     3541 aaagattccc cctaaaagtc caaaaattat cagagttaaa aaagaaatat ctttaataaa
     3601 aacagcctta actaaagcaa taagtaaaat tccccctaaa agtccaccca gagctccttc
     3661 aatggtcttt ttagggctga tttttgtata gtttgttttt ccaaaatatg ttcctatata
     3721 ataggcaaag gtatcattgc cccaagctaa aaccagtgcc agggtataaa ggtactttat
     3781 gggagaaaag gcaatcaatc tgtaaagaag taaaaatggc aagaaagtaa aaaaataagc
     3841 aaaactattt aaatacaccc tttttaaatc caatgggttc atgataagac ttaggcctaa
     3901 agaaagaact atgtataaaa aacccaatgg caaagcaaga tcccaaggta aagaaaaact
     3961 taaaacaaca aaaatagcac taataaaatc atccaggcga aaagatgaac ctaaggttcc
     4021 ttttaattcc atcatgcaaa gaagtgtaag cataattagg ccaattcgca agggtaaacc
     4081 gcctatatat aagagtataa aaagtaagga aataccaaca attgcactaa ttactcgctt
     4141 attcattatc tattcctcca aaacgtcgtt tgcgattaga gaattcttct aaagcaatta
     4201 aatattgttt cttatcaaaa tctggccaat atattggact gaaatataat tcgctatagg
     4261 ctaattgaaa aagaaggaaa ttgcttattc gaaattcacc actggtacga atgataaaat
     4321 caggatctgg cattcctgct gtatagagat actccttaaa gctctcctca tcaacttgct
     4381 tgatattatt ctccaaaaga aggttcactg ctctaattat ctctgctctt cccccataac
     4441 ctagggcaag acaaatatga atttcatcat tgttttttgt agtttgcata gctttttcca
     4501 aaacaaaacg aacgggtgca gaaaatagag ataggtctcc taaaatatgg atacatgccc
     4561 cttctttatg cagctccggt agttctcgat taaaaaactc gattaaaaga cccattaaag
     4621 tattaacttc ttttagaggt cttttaaagt tttctgtact aaaagcataa aagctcaagt
     4681 atttgatttt taaatctttg cttgttttta taatggttcg aatgttttct gttcctgctc
     4741 tatggccatc acttcgtgat aagcctttgc tctgtgccca ccgtccattt ccatccatta
     4801 taaaaccaat atgtttagga agcattacac cgccattatt tcattatttt tttcatcaac
     4861 aagtcggtcc acttcttcga cagctttatc gacgattttt tggacatctt cttgagcacg
     4921 aatcgattca tcttctcgga ttaggctgtc tttttcttcc tttttaattt gctcaattgt
     4981 actacgtcgt tcattgcgta tggttacctt agtttcttcc ccaagttttt taaccagttt
     5041 agaaagcttt attctgtttt cttcagtaag caggggaaga tttagacgta caatcttacc
     5101 atcattattt ggattaagag aaaggtcaga agttaaaatt gccttctcaa tgttagaaat
     5161 tgatgtttta tcatagggtg aaatgacaag aagtctagct tctggagcgg ttatggtggc
     5221 aagttgattt agtggcgtat ctaccccata ataattaaca acgatacgat ctaaaattcc
     5281 aggatgagct ctcccagctc ttacattgga gagttcgctt ttgaaaaaat ccagggcttt
     5341 attcatttgt gcttgtaaat ttcgattgta atcttccatt gttttacctc ctgataatag
     5401 ttccgtcttc ttggctttca agtgctcgta ataaactatc ctgcttattc attgcaaaaa
     5461 caaaacacgg tatcttgttt tcagctaata gggttgctgc agtttgatcc ataaccttta
     5521 agtccccgat taaaagatct ctgtaatcca tctcacgata acgcttagca tctgcattaa
     5581 attttgggtc cttatcatat acaccatcta ttgttttagc aaaaagaaca atctctgctt
     5641 caatttcagc cgctcgaaga gccgctgttg tatctgtaga aaaaaaagga ttaccggttc
     5701 caccagaaaa aataacaata cgattttttt ctaaatggct gacggccttc ctgcgtaaat
     5761 aaggttcagc tactttaggc atttcaatag cgctcataac tcttgttggg atagaaagct
     5821 tttcgattga gtcctgaaga gcaaggccat tgattactgt tgccagcatg cccatatagt
     5881 cggcagttga gcggtccatc tctttgctac ttcgccccct ccaaaagtta ccacctccga
     5941 ccacaataga aatttcataa ccattttcat acagctctgc tatttgactg gcaatttctg
     6001 ccagtacatg atcatctata cctgtacctg aggcgggggc tagtgcctcg ccactaagct
     6061 taagaagtat tcgtttgtat ttcaacttaa tcctccgcaa attgtttata atctaatagt
     6121 aattgtaaca tctttaaagt aaatcatcaa gcatgagggg taaaaaaaga agccctcaaa
     6181 tgaaggcttc aacttatgcc atttgttttg ctacttcttc tgcaaagttc tctactcttt
     6241 tttcaagacc ttctccaact tcataacgaa ccatgcttac tacttcaatt ttttcaccaa
     6301 gggttttctc gatattttta accaaagcac caactgttaa atcactgtct ttgataaaac
     6361 tttgttctaa gagacatacc tcttgcagtt ctttattaag acgaccttca accatttttt
     6421 caataatgtt ttctggttta ggttttttac cagcagcttc tgcttggcgg ttctcattta
     6481 aagcttgttc taagagtatt ttcttttcac tatcaatata gttagcgtca acatcatttc
     6541 ttgaaatata ttttggactc atcgaagcaa cttgcattgc caagtctttt ccaagttcag
     6601 caagatcagc tgcagcagca ttgcttttaa agccaactaa ggctccaatg cgaccatttc
     6661 cgtgaagata gccaaaaacg ctgccagaat ccacttcaag atgtttgaag cgtctgatat
     6721 tcatattttc accaatacga gcaattaatt ctgtcaaaaa ctcgctaaca gtcttatctt
     6781 caatcctcag actattaaga tcttccattt cagcaggttt ttgcttatgg acaagggcag
     6841 caagtgtttc aacaaaacca ctaaaatctt tgttttttgc tacaaagtct gtctcagcat
     6901 tgacttctac tagagttccc gcagcattgt cttcatcaag aaccatggct acaagacctt
     6961 cagctgcaat acggcttgat tttttctctg agcttgcaat gcctttttta cgaagatatt
     7021 caacagcggc ttctatatct ccattgtttt cttcaagtgc ttttttacaa tccagcatac
     7081 cagcacctgt gcgctggcgt aattctttta tctcttttgt tccaaaggac atatattttc
     7141 ctcctacttt acgattattc tttatcaacg gaaacgtctt cttctatttc ttcttcttta
     7201 tttgcttcct gagttacttc ctgctcttca agcatttgct catcttctgc cgcgtccatg
     7261 gcagcttttc cttctaaaat agctgtacta atggtagcgg aaaacaattt aatggcacga
     7321 attgcatcat cattaccagg aattggataa tcaatttcat ctgggtcaca gtttgtatct
     7381 aggattccta ctacaggaat tccaagaagc ttggcttcat gaatagcgat cttttcttta
     7441 ttttggtcaa taataaaaat tgcatcagga agttctctca tatctctaat tccattgaag
     7501 aagcgttcaa gtttgtcttt ttctctgcgt aggagaataa cttctttttt gggtagagct
     7561 tcaaaggtac catcagcttc catttcttct agttcgaaga gataatcaat acgcttacgg
     7621 atcgtttgaa agtttgttaa aaggccaccc ttccaacttt gattaatata atacattcca
     7681 gctcttttag cttcttgctc tatggcaata cgcgcttgtt tttttgtccc tacataaaga
     7741 actttaccac ctttactggc caggtctcta ataaaatcac aggcttcttc caacatttta
     7801 acggattgtt gaaggtctat gatatagatt ccattacgct cagtatagat gtaaggcttc
     7861 attttaggat tccaacgttt tgtttggtga ccgaagtgaa ctccggcttc caatagactt
     7921 ttcattgata ctacggacat aatatttcct ccttgtttgt cctccccacc cgattaccat
     7981 aacggcacaa gcagatttac caggtaggtg tgtatttaac ctcatgtatc ttatcataag
     8041 aaatgcatta tatcaatact ttttggctat ttacaggata attatttatt gagctgactt
     8101 tgataataat ttctttatct gcttaactga taaagctttc ccaacagact taacctcgtc
     8161 gttgattaca aaaccaggag tgctcataac cccatagctc acgagttcaa caaggtcttc
     8221 cacattttct atccttgcct caatattcaa ctcttttaag gcaatttcca agtttttttc
     8281 taacttttca cattttgtac agccagttcc tagtattttt atttccatta aattctcctt
     8341 ttatagtaaa aatggggaaa gagcatttag tccatagcca attattataa tcccaataac
     8401 aacaacaaag acaaagcttg ctaacagttt tgcttttacc accttgctaa gcataactag
     8461 agaaggaata gacattgtgg taactgccat cataaaggat ataaccgttc ctatgccaac
     8521 ccccttagaa attaaggctt ccgctatagg gatggtacca aaaatatcgg cataaattgg
     8581 tactccaatt attattgcaa ttaaaacaga aaagggattt tgatttccta gtattttttc
     8641 aacgatgctt tgagggagcc agttgtggat gccagcacca atgccaacac ctataagaat
     8701 ataaggccaa actcttttga taatatcctc tacttgttct aaagagaagt caaggcgctc
     8761 ttttctagtc atttcatcta gttttacttc tttggcttca acaccccaga cataggactc
     8821 tacataattt tccatttgca tcttatgaat gataattcct cctaaaattg caaccccaac
     8881 cccagcagct acataagtaa aagcaatctt aattccaaaa aaagataaga ggagcataag
     8941 agcggccaaa tcaatcatgg gagaagaaat aagaaaggaa aaggttgctc ctagcggaag
     9001 tcccgctgag acaaatccta taaatatggg aatgctagaa caggaacaaa aaggagttag
     9061 tatccctaat aaagccccta ttatattgcc tctaattcct ttaatacctc ctaggagttt
     9121 cttggtgcgc tctggaggaa aataactttg tatataggat ataaaaaaaa tcataacagt
     9181 aagtaggata aaaatcttaa ttacatcata gatgaaaaaa tgaatggatc cacctactct
     9241 tgtatccata gaaagattaa atatttttac caccaagatt tctactacat cccatagcca
     9301 tttcattttt aggaactggt catttaaaaa gcttagtatt gtcataacct cacctttaag
     9361 ccttatttgc ttcccttaat tcaatataaa tcgcagcagc tttacttttt tctaaatcca
     9421 acttcaaaac aggatactca tcaataccct gaacgatctc tgagagtatc ttgttaaaaa
     9481 gtgtatcttt aaggtttagt gaataaaatg tatattgttc ttttctttga tcttttacca
     9541 gtccaatatc acgaaggcgg gctagatgtt tggacacatt gggttgcgaa ataccagtta
     9601 taccagttat ttggcaaacg caaaattcat cgtgataaag aagcaccata attctgagcc
     9661 tggtttcatc tgaaagaagt ttaagataat ttattagatt ttgcatttta actcctattt
     9721 taatctcatt tttctaccga caacactctc ccaggcttta aagatgggcc ttatgatgtg
     9781 aacacctatt cgggagataa ttaccagttc ggaatttggc cagacttgat cggttttttt
     9841 atagtcaatc cttttaccaa caacatcaat ttgattccaa ccatcttcta gttcaaaaaa
     9901 gccctttatt cgatagctgt cctctttaat taaatcaaga aactgtccca ttttttcctt
     9961 tgttaaggag ccctcgtagg taagtgttag ggttttaggc ctgttttcct ttgtattggt
    10021 ggtctcttca ctgcctatcc agtttttttc cattagattt ttatcaagaa aactgtagtt
    10081 tacaattccc ttttcagatt ccacaagatc aacattttta ttgatttctc taatttttcc
    10141 ttttacttgc tcaagtgttt cttcacttat gagatctgct ttagatataa tcacaagatg
    10201 agcaaatttt agctggcgct gaactgtttc aatatctttt atttggtcta aaaaatttac
    10261 tccatcaaca atacataggg ccccagaata atcataaact tctcccttag ctatcataac
    10321 agcatctaaa aactcatcta tattggatgg atctgcaaga ccagagcttt caacaaataa
    10381 gtaatccata tcgcgattag cgatttcaac aagagcggag acaaaactta gctgcagaca
    10441 agaacagaaa atcgatccgc tattaagttc cagtagttcc atgccctctt tttgaatgat
    10501 tgggccatca atgccgacct tgccaaattc attcatgatt acagcaacct tactcccctc
    10561 taggtcctct agtacttttg taagaaaagt agttttacca gctccaagaa agcctgtaat
    10621 taaatataat ttcgtcttct tagacatata ttccccctaa tttggtttac atatgcttgc
    10681 ataggcatat gcgtatatta gaataataca tcttttgctt ttctttgtca atcattaaat
    10741 aatgcctttt taatacttaa aggtaatttg tagcaaaaaa aataggccat aatagcctat
    10801 tctgtttttt tgtcctctac atagtcacat tctttattgc tacaatgtat ggtatctttt
    10861 ctcctaccct ttccttctac aaggatagaa tcacatttag gacatttcct atcaacgggt
    10921 ttgtcccaag ttgcaaatcg acattctggg aattgatcac atccataaaa tatacggagc
    10981 tttttggttt tgcgctcaac aatttgtccg ttttcacaaa ggggacaagc tattccaatc
    11041 ttttttaata ttggttttgt atttttacat tcaggataat tgctacaggc gtaaaagctc
    11101 ccttttattg tttttttaat aagcattttt gaaccacatt tatcgcagtc aatatctgta
    11161 agttcattta gatcatattt ttggatacgc ttttccgctt cttctagcat tggctgtaag
    11221 atatcgtaga aatcaccaat aacttcttgc caagaaactt cctcattgct aattttatct
    11281 aattcttctt ccatcttggc agtaaattct ggttcaacaa tttgaagaaa attttcttcc
    11341 ataatctcat tggttatttc accaagttca gtaggcttta aagatttttt ctcttttact
    11401 acatagcctc tttttacaac agctgaaatg gttggtgcat aagtactggg gcgaccaata
    11461 ccattttctt ctaattcctt tataatactt gcttccgtat atcgagccgg tggttgagtg
    11521 aatttctgtt cctctttaaa ttttttaaga agagtatttt caccttcctt taagggtggc
    11581 aaatcgatat ccttagtgtt tcttgcatca tagactttaa gaaaaccatc aaagacaagg
    11641 cgttctcctt ttgtcctaaa cttatagtct cctccactta tagtaatttc caagccttca
    11701 aactgggcct gtttcatttg actagctaca aagcgctgcc agataagcgt atagagcctt
    11761 aattcatctc ttttaagata ggaagccatt ttctccggtg tatgttcaac tgacgtgggt
    11821 cggatcgctt catgggcatc ctgagcatcc ttgcctgctc cccctttttt tggagcagaa
    11881 tattttttgc catagttttc aacaataaaa tccttacagg aacttacagc tgtttcactg
    11941 atcctggaag aatcagtacg catatatgtt ataagtcctt caacactttt tccaacccga
    12001 accccttcat agagtttttg ggctaccatc attgtttttc ctgatgaaaa attaagtcgc
    12061 actgaggcat cttgctgaag tgtacttgta gtataggcag aatagggaaa tctccttctc
    12121 tttttttctt cgatactgct aatagaaaag tttgttgatt caatttcctt cttaattcgc
    12181 atagcttctt cttgcgaaga aacatcaatt tttttatctt tatatctcca aagatctgcg
    12241 ctaaaggctt ttttgtcttc ggataaaaat tttcctgtaa aaacccagta ttcttcgggt
    12301 ttaaatgcct ttattttacg ttctctttga ataataagcc ttgtagtaac agattgtacg
    12361 cggcctgcgc ttaagccttt cataacttta cgccatagaa ttggacttat ctcataacct
    12421 accagtcgat ctaatattct gcgaccttgt tgggaatcaa ctaggcgctg atcaatatgg
    12481 gtaggattct tgatcgcttt cttgatagca tccttggtaa tttcttgaaa tgttacccga
    12541 atgggttctg aatcatcgag tttaagggct gtagccaaat gccaagcaat ggcctctcct
    12601 tcacggtcag ggtcagtagc tattaaaaca ttctgggctt tctttttttc ctttttaagt
    12661 tctgcaagga cgggaccctt tccccttatt gttatatatt tgggtttgaa attgttctca
    12721 atatcaattc ccatttggct ttttggtaaa tcacgcacat ggccaacact ggccattact
    12781 ttataattct tacctaaata gcgttgaatt gtttttgctt tagtgggtga ttcaacaatc
    12841 accaaatttt ttgccatagt tcttcctccc aatggtcaat tagatcataa tgataaaggg
    12901 ggtttgcccc ttgaaatatc aagtaattag gaccaaggct taaggggtct tcgggtgaac
    12961 caggaacaac ccagagatct tttccttgac ctaaggcttc tttagcggta atcatagttc
    13021 cgctttttat ttcagcttca actattatcg taataggact caaagcggca agaagacgat
    13081 ttcttcttgg gaaatgatgg ggtctaggcc ctacattagt aggatattca gatagaagaa
    13141 gtccttcttc ttccatacgc catcttagta aatctctccc cctaggataa aggctattaa
    13201 gaccagttcc tagtatagca atactctttc caccaaactc aaaggcagat ttatgagcca
    13261 tttcgtctat gccctttgca aatccactga ctatcactat atcacgggcg actaattttt
    13321 caactatttt ccgtgtttga gcaaggcctc gctttgtggc ttttcgactt ccgacaatag
    13381 ttacgcattt tttttcaagt aaatcaaggt ttccttgagc atataaaagc ttaggtagct
    13441 catgtaaaaa taaaagtccc cgaggaaact tttcctgttc tataatcata atacctccac
    13501 caccctagta taactacttt gagcttagag gtagacttat tatttaaggt tgtcattaag
    13561 gaaagaaaga aaactttttc gatgaaggtg gcaaggccca aattctctta aagcctctat
    13621 atgttgtgct gtaccatatc ctttatgctg ggcgaaccca taaacaggga aatgtttatc
    13681 catctccatc atccaatgat ccctagtgac ttttgcaaga attgatgctg cagcaatttc
    13741 attacttaaa tcatctcctc ctatcaaggc ccattccata ggatgaagat ccattggggt
    13801 cccatcaaga agaagggcat ccaagctaaa accaagtttc ttaatggctc tttgcattgc
    13861 cagctttgtt gcatttaata tatttaattt atcgatttct ttttggtcgg cccatccaac
    13921 accataacc
//
LOCUS       DASRNA010000240         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_522041,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000240 DASRNA010000000
VERSION     DASRNA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522041"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..2358)
                     /locus_tag="VFD08_06595"
     CDS             complement(289..2358)
                     /locus_tag="VFD08_06595"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HZK10723.1"
                     /translation="MYKVVKRDNQIVAFDIEKISAAIEKAFQSRNKNYHQSVIDLLAL
                     RVTAEFEDKIIDKKISVEDIQDCVEVVLIQAGYADIAKAYILYRKQRENIRNIKTTFL
                     DYKEIVEDYVHINDWRVKENSTVTYSVGGLILSNSGAISANYWLSEIYDEEISQAHKE
                     ADIHIHDLSMLTGYCAGWSLKQLIEMGLGGIPGKITSSPAKHLSTLCNQMVNFLGIMQ
                     NEWAGAQAFSSFDTYLAPFVKVDKLSYKEVKQCIQSFVFGVNIPSRWGTQSPFSNITL
                     DWTVPQDLADLKALVGGREMDFTYRDCQEEIEMINRAFIEVMLEGDANGRGFQYPIPT
                     YSITKEFDWSDTQNNRLLFEMTAKYGTPYFSNYVNSEMQPSDVRSMCCRLRLDLRELR
                     KKSGGFFGSGESTGSIGVVTINLPRIAYLSKDKKSFYQRLDKMMDLSARSLETKRKVI
                     SKLLKEGLYPYTEHYLGSFDNHFSTIGLIGMNEVGLNASWLGENMASGKTQEFSKEVL
                     NHMRDKLSDYQEKYDVLYNLEATPAESTSYRLAKHDKKRYPGIQTAGEKAGVPYYTNS
                     SNLPVDYTSDVFDALDIQDEIQTLYTSGTVFHAFLGEKLTDWKAAATLVRTIASNYKL
                     PYYTLSPTYSICGAHGYINGEENICPICGDKTEVYSRITGYYRPVQNWNDGKSQEYKD
                     RRVYAMD"
     gene            complement(2438..>2923)
                     /locus_tag="VFD08_06600"
     CDS             complement(2438..>2923)
                     /locus_tag="VFD08_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HZK10724.1"
                     /translation="EPLRQKKLLDFISKVKELGYLVKLDTNGFYPIKLEKLIEDGLLD
                     YIAMDIKSSADKYALATGVDKVDVKKVNQSIELLQRNQVAYEFRTTAVKGIHTLEDFE
                     KIALWIEASPRYYIQNFVDSADILSFRREPKTSLEPFSKEELEEILQVVKKRLPQAQI
                     R"
BASE COUNT          773 a          610 c          504 g         1036 t
ORIGIN      
        1 ctattaaata gtacccccaa taggctcttt taaaaggcct tttgcataaa gacttagatc
       61 ccctccttaa aaaagcttat aataaagacc ctggtcaaaa tcattcctgt atttttatag
      121 tagtatcttc cttttataac agtttatagg ccccatatag ggcttaatca agttaaagaa
      181 ttcaaaaaat ggacccaatc tgcttatata agggtccatt tctttgacta tagtagtttt
      241 ttatagatac cacctttaaa aattaaagtg atttcttctc tttttttatc aatccatggc
      301 ataaactctt ctgtccttat attcctggct ctttccatca ttccagtttt ggaccggacg
      361 atagtagcct gtaatcctgc tgtatacttc tgttttatct ccacagatag ggcaaatgtt
      421 ttcttcccca tttatatagc catgggctcc acaaattgaa taggttggag ataaggtata
      481 gtagggtaat ttataattgc tggcaatggt tcttactaaa gttgctgctg ccttccagtc
      541 tgtgagtttt tctccaagaa aagcatgaaa aacagtgcct gaagtataga gggtttgaat
      601 ctcatcttgt atatcaagag catcaaaaac atcgctagta taatccacgg gtaaattgct
      661 tgaatttgta taatagggca ctcctgcctt ttctccggct gtttgaatac caggatatct
      721 ttttttatca tgcttggcta gccggtagct tgtagactct gctggagttg cttcaagatt
      781 ataaagaaca tcatatttct cctgatagtc tgaaagctta tctcgcatat gattgaggac
      841 ttctttagaa aattcctgtg ttttcccact ggccatattt tcacccagcc aactggcatt
      901 tagacccact tcattcattc ctattagacc aattgtagaa aagtgattat caaagctgcc
      961 tagataatgt tccgtatagg gatataggcc ttcttttaag agctttgaaa ttacttttct
     1021 ttttgtttct agtgatcttg ctgagaggtc catcatttta tctaagcgct ggtagaaact
     1081 tttcttatcc tttgaaagat aagcgattct gggtaggttg atggttacaa ccccaatgga
     1141 accggtgctt tctccacttc caaaaaagcc gcctgatttt tttcgcagct ctcttaaatc
     1201 caaacgaagt ctgcaacaca tgcttcttac atcacttggc tgcatttcac tgtttacata
     1261 gtttgaaaaa taaggggttc catattttgc tgtcatctca aatagcagcc tattgttttg
     1321 cgtatcggac caatcaaatt ccttggttat agaataggtt ggtatgggat attgaaagcc
     1381 cctcccattg gcgtctcctt ctagcataac ctctataaag gccctattga tcatttctat
     1441 ttcttcttgg caatctctat aggtaaagtc catttccctt cccccaacca aggccttaag
     1501 atctgctagg tcttggggca cagtccagtc caaagtgata tttgaaaagg gtgactgggt
     1561 cccccatctt gatggtatat ttacaccaaa aacaaaggat tgtatgcact gcttgacttc
     1621 cttataagat agtttatcta ccttaacaaa gggggctaga taggtgtcaa aggaggaaaa
     1681 tgcctgggca cctgcccatt cgttttgcat aattcctaaa aaattaacca tttgattgca
     1741 caaggttgaa agatgcttgg ctggagaaga ggtaatctta cctggaattc cacccaggcc
     1801 catctctatg agttgtttta aagaccatcc agcacagtag cctgtaagca tagacagatc
     1861 atggatatgt atatcggctt ccttgtgggc ctgggagatc tcctcatcat agatttctga
     1921 tagccaataa ttggcactaa tggcacctga attgcttaag attagccccc ctacagaata
     1981 agtcacagta gagttttctt ttaccctcca gtcattaata tgcacatagt cttctacgat
     2041 ttccttataa tctaaaaaag ttgtcttaat atttcttata ttttcccgtt gttttcgata
     2101 caatatatag gcctttgcta tatctgcata acctgcctga attaagacca cttccacaca
     2161 gtcttgtata tcctccactg agatcttctt gtctatgatt ttatcttcaa attctgctgt
     2221 tactctaagg gccagtagat ctatcacact ctggtgataa tttttatttc ttgattgaaa
     2281 ggctttttca atggctgcac ttatcttttc tatatcaaat gcaacaattt gattgtctct
     2341 tttaaccact ttatacattt ttctctcctt ttaagcttct tcttttcttt gggggctgtg
     2401 tagttacttt tagttttttt tagcttttct tttcttccta gcggatctga gcctggggga
     2461 gccgcttttt tactacttgt aaaatctctt ctaactcctc tttgctaaag ggctccaaag
     2521 aagtctttgg ctctcttcta aaagagagga tatctgcaga atccacaaag ttttgtatat
     2581 agtatctagg ggaagcctca atccataggg caatcttctc aaagtcctct agggtgtgaa
     2641 tgccctttac tgccgttgtt ctaaattcat aggcaacttg atttctttgt aaaagttcta
     2701 tgctttgatt taccttcttt acatctactt tatctactcc tgttgccagg gcgtacttat
     2761 ctgctgagct cttgatatcc atggcaatat aatcaagcaa gccgtcttct ataagctttt
     2821 ctaattttat tggataaaag ccattggtgt ctagctttac taggtatccc agctctttta
     2881 ccttactgat aaaatctaat aatttctttt gtcttagtgg ttc
//
LOCUS       DASRNA010000241         7231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10230 k141_895217,
            whole genome shotgun sequence.
ACCESSION   DASRNA010000241 DASRNA010000000
VERSION     DASRNA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963756
            Sequence Read Archive: SRR5678807
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:12:23
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,329
            CDSs (total)                      :: 1,316
            Genes (coding)                    :: 1,304
            CDSs (with protein)               :: 1,304
            Genes (RNA)                       :: 13
            tRNAs                             :: 11
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895217"
                     /isolate="SMAG_U10230"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VFD08_06605"
     CDS             <1..637
                     /locus_tag="VFD08_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010053469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HZK10725.1"
                     /translation="DIITADIISREHSIKLLIVLMAIFSLMLLIGLFQQNWSLREYDK
                     LLNEIGRLVTDFTHLYGFGLTLINMAFMGFLATAYVLISGGPINGPIVGGIFTIVGFS
                     AFGKHPKNTLPIFIGVYLALLLNVYDRSSTSSTLGALFGTTLAPIAGRYGLIPGIITG
                     FLHAGMVTNVTYLHGGVNLYNNGFSGGFIAASLAPIFEKVEVIIDEQKRKI"
     gene            615..956
                     /locus_tag="VFD08_06610"
     CDS             615..956
                     /locus_tag="VFD08_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10726.1"
                     /translation="MNRREKYKARKIVDELTGYLLRNNINSLSIYLDFNEERVEISLW
                     GQLTKEIPNLETLISLMNARRVPEMEDYYEQLLGTGIDDNDINILGAMVDKATYTLKE
                     DRLEIKVVRYL"
     gene            953..1399
                     /locus_tag="VFD08_06615"
     CDS             953..1399
                     /locus_tag="VFD08_06615"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZK10727.1"
                     /translation="MTDKDYMALALEEAKEAFLEEEVPIGAIVVRDGEILGKGHNEKE
                     QRRDATAHAEILALKEAMKKTGAWRLTGATIYVTIEPCVMCVGALLQARVKRIVFGAP
                     DPKFGGVVSLHRLADDPRHNHRCLYTGGVLADEAKSLMQDFFKARR"
     gene            1400..1487
                     /locus_tag="VFD08_06620"
     tRNA            1400..1487
                     /locus_tag="VFD08_06620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1433..1435,aa:Ser,seq:gga)
     gene            1491..1753
                     /gene="ffs"
                     /locus_tag="VFD08_06625"
     ncRNA           1491..1753
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFD08_06625"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1838..4507
                     /gene="secA"
                     /locus_tag="VFD08_06630"
     CDS             1838..4507
                     /gene="secA"
                     /locus_tag="VFD08_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZK10728.1"
                     /translation="MGFLDFFKKRLGQGELDRLGNQTEEIQSIIPSMEKLSDEALQEK
                     TIEFKERLKKGETLDDLLPEAFALVCEASKRTLGMSPYPEQIMGAIILHEGRIAEMKT
                     GEGKTLVATMPLYLNALEGKGAHLVTVNDYLASYQSQWMGQLYQFLGLSVGCLTNDLK
                     GEDRHFQYAADITYGTNNEFGFDYLRDNMAIYQSSVYQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQGNKSTDLYGKAEGFTKTITKDDVIIDEKANTIMLSEEGGMDKAEKYFSVD
                     NLADMDNMEIYHHINQAIRARFLMKKDVNYVVKDGEIVIVDEFTGRLMPGRRYSSGLH
                     QAIEAKEGISIRKESQTLATITLQNFFRMYSKLAGMTGTAKTEEEEFREIYGMDVVEI
                     PTNRPVQRKDLGDVIYGHSAYKYKAIIEEIIQRHQTGQPILVGTVSIETSEYISKQLK
                     KAGIPHEVLNAKHHHREAEIIAQAGRFGAVTIATNMAGRGTDIMLGGNLEFVLRKELR
                     KRDFEDDVISYALSPFDYEEEEVIKAKSLIKELRGPIVDEINKEAERVKNAGGLHVLG
                     TERHEARRIDFQLRGRSGRQGDPGSSQFFLSLDDDLLRIFMSENVIERMRSLGFGEDM
                     PIQHKMLTKSIESAQKKVEANNYGIRRYVLQYDQVMNEQRRHIYGNRNAVLNNENQRD
                     NVMDMARAYIEDYIDMNRDLSPLVKDWDVKSLDSQLKNVFFEDTSFLDELPKDCDAEG
                     LQEFLNQHIEEFYQKREELYGIETMRGVERAVILSVVDQNWIDHIDAMDSLKQGIGLR
                     AIGQIDPVQAYQIEGFAMYEEMIDGIREETLRILFKASFKEGSEQRKDMAVVTGESSG
                     QKTTAQRRVKKVGRNDPCPCGSGKKYKYCCGRN"
     gene            4510..5599
                     /gene="prfB"
                     /locus_tag="VFD08_06635"
     CDS             join(4510..4578,4580..5599)
                     /gene="prfB"
                     /locus_tag="VFD08_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148206931.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZK10729.1"
                     /translation="MQELCRSKLGKLREKWMQIRESLDPEKVKSKYEELTKLMMEEDF
                     WSVPEESTEVQIEHKHLKNQLEMIASVDELLEVCELSCELFELGEGQNKEGISYFKSF
                     EKKLDELEISTLLSDKYDGNGALLSIQAGTGGLDAQDWAEMLYRMYRRYLSQEDFSFE
                     VLDFLDDEAAGIKHVTLLVRGYYAFGKLKSESGVHRLVRISPYNSSGKRQTSFASVEV
                     YPEIKDSGEIQIDEEDLKIDTYRSGGAGGQHVNTRDSAVRISHLPTGIVVQCQNERSQ
                     ISNRETAMRMLHSKLIEKKMREDKQKHDKLKGEAKVIAWGSQIRSYVLHPYTMVKDHR
                     TGEENGNVQAVLDGDLDPFIKAYLQQNI"
     gene            5609..6748
                     /locus_tag="VFD08_06640"
     CDS             5609..6748
                     /locus_tag="VFD08_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZK10730.1"
                     /translation="MKVIKNVKVLTMEGPTEVYEDVDVAIENGKIKEIGKDLTGDEII
                     DGEGHFLLPGFIDAHCHLGMWEDAIGFEGSDGNEMTSPITPQLRGIDAINPMDRTFDD
                     AISGGVTSVSTGPGSANVMGGTFTAIKTAGKRIDDMIIKDPVAMKIAFGENPKRVYNE
                     QKKSPSTRMAVAAELRDVLFQAKNYMEKREKNGDQGVELKYEALLPVLKKEIPLKAHA
                     HRADDIFTAIRIAREFDLDITLEHVTEGHLIADELAKENLSCVVGPSFGARTKFELIN
                     KSFITAVKLQEAGVKVAIMTDHPVIPLENLNLCAQFCVKEGMDHYEALKAITINPAEI
                     IGIEERVGSIKVGKDADLVLWDGDPLQVDSRVLLTMIDGKTVYKA"
     gene            6884..>7231
                     /locus_tag="VFD08_06645"
     CDS             6884..>7231
                     /locus_tag="VFD08_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK10731.1"
                     /translation="MKKIHEAWKTNLIFLLFMIFSLLYKELILRSLIGYGGKYLFLFT
                     GVFPLFLMGIFLISRGKWRYIVADIYFLGLSLLFISQALYFQFFTNLYSFYLLSGAGQ
                     TARNGDEIIRLILN"
BASE COUNT         2366 a         1230 c         1679 g         1956 t
ORIGIN      
        1 tgatattata acggcagata ttatttctcg ggagcatagc attaaacttc ttattgtact
       61 tatggctatt tttagcctga tgcttcttat tggcctcttt caacaaaact ggagtttgag
      121 agagtatgat aaactattaa atgagatagg taggctagtt actgacttta cccacctcta
      181 tggttttgga cttactttaa ttaatatggc ctttatgggg tttttagcta cagcctatgt
      241 acttatatcg ggaggtccca taaatggacc aattgtaggg gggatattta ctattgtagg
      301 attctcagcc tttggcaagc atccaaaaaa cactcttcct atttttattg gagtctatct
      361 ggccctgcta cttaatgtat atgatcgaag tagcacaagc tctaccctag gagcactctt
      421 tggtacaacc ctagcgccca tagctggccg ttatggatta attcctggca ttatcacagg
      481 gtttttgcat gcaggcatgg taactaatgt tacttacctt catggtggag tcaacttata
      541 taacaatggt ttttccggtg gttttattgc tgctagtttg gcgccaattt ttgagaaagt
      601 agaagtgatt atagatgaac agaagagaaa aatataaagc aagaaaaata gtagatgaac
      661 ttacaggata tcttttaaga aataatatca atagtttaag tatctacctt gattttaatg
      721 aggagagggt ggagatttct ctctggggac agcttaccaa agaaattcct aatcttgaaa
      781 cactaatatc ccttatgaat gctagaagag ttccagagat ggaagattat tatgaacaac
      841 tcctgggaac aggcattgat gacaatgata tcaatatttt gggagccatg gttgataagg
      901 ctacctatac cctaaaggag gatcgcctag aaataaaagt ggtgcgttat ctatgacaga
      961 caaggactat atggccttgg ccttagagga ggcaaaagag gcttttttag aagaagaagt
     1021 ccctatcgga gcgattgttg tgagagatgg ggaaatattg ggtaaaggac ataatgaaaa
     1081 agagcaaagg cgtgatgcca cagcccacgc ggagattttg gccctaaaag aagcgatgaa
     1141 aaaaacggga gcttggcgct tgactggtgc tacaatttat gttaccattg agccctgtgt
     1201 gatgtgtgta ggagctctgc tccaggcaag agtaaagagg attgtatttg gtgcaccaga
     1261 cccgaaattt ggaggggttg ttagtctcca tagattggct gatgatccaa ggcacaacca
     1321 tcgctgccta tatacaggtg gagttttagc cgacgaagcc aaaagtctaa tgcaggattt
     1381 ttttaaagca agaagataag gagaggtgac cgagcggcta aggagcgcgc ctggaaagcg
     1441 cgtagggtgt aacagcctcg cgggttcaat tcccgtcctc tcctccattt ccgtgctagg
     1501 cggggcacta gcggtaccct gtaacctaca atccgctata gtagggtcga acaccttttt
     1561 gagggacctt gaggtggagc tgctcttgga aagcaccaat gaaggattag tcctacgcaa
     1621 tgaagacttt tgaaccccgt cagatctgga aggaagcagc ggtaagaaag aaactccatg
     1681 tgccgtggga gcgctgatcc cgagggaact accaagatac cgtctggttt aaggtttcga
     1741 tggaggcgca cggtttttaa gctcttagga gcttttttta tttagaaatg taaagaaaaa
     1801 tataaacaaa caggaataat gataaaaagg agaagcagtg ggatttttag attttttcaa
     1861 aaagcgtctc ggccagggag aactcgatcg ccttggaaat caaacagaag aaatacaaag
     1921 tattattcct tctatggaaa agctaagtga tgaagcttta caagaaaaaa caatagaatt
     1981 taaagagcga ttaaagaagg gcgagacctt agacgatctc cttcctgagg catttgctct
     2041 tgtttgcgaa gcatcaaaaa gaacattagg aatgtcgccc tatccagaac aaattatggg
     2101 ggccattatt ctccatgaag gaagaattgc agagatgaaa acaggcgagg gtaagaccct
     2161 agtggccacc atgcctcttt atctaaatgc attagagggt aagggagccc atcttgttac
     2221 cgtaaatgat tatcttgcat cttatcaaag tcagtggatg ggccaactct accagttttt
     2281 aggtttaagt gtaggatgtc ttaccaacga cttaaaagga gaagaccgcc atttccagta
     2341 tgctgcggat attacatacg gaacaaacaa tgaattcggc tttgactatt tgcgcgacaa
     2401 tatggctatc tatcagagtt cggtctatca aagaccactg aattatgcca ttgttgatga
     2461 ggtagactct attcttattg atgaggcaag aacacctctt attatttctg gtcaaggcaa
     2521 taaatctacc gatttatatg gaaaagccga gggatttact aagacaataa caaaagacga
     2581 tgtaattatt gatgaaaagg ccaataccat tatgctctca gaagagggcg ggatggataa
     2641 ggcagaaaaa tattttagtg tcgacaatct tgctgatatg gataatatgg aaatatacca
     2701 ccatatcaat caggccattc gggctcgttt cttgatgaaa aaagatgtga attatgtggt
     2761 caaagatgga gaaatagtga ttgttgatga atttactgga cggcttatgc ccggaagacg
     2821 ttattccagt ggacttcatc aagcaataga ggccaaagaa ggaatttcca tcagaaaaga
     2881 atcacaaacc ctggccacca taaccctaca aaactttttt agaatgtatt caaaacttgc
     2941 cgggatgaca ggaacagcca agaccgagga agaagaattc cgcgagattt atggaatgga
     3001 tgtggtggaa atacccacaa atcgtcctgt acaaagaaaa gacctaggcg atgtgattta
     3061 tggacatagt gcctataaat ataaggccat tattgaggaa atcatccagc gtcatcagac
     3121 gggccagccc attcttgtgg gaacggtctc tattgaaaca tcagaatata tatcaaagca
     3181 acttaaaaaa gcggggattc cccatgaagt gcttaatgcc aagcaccatc atagggaggc
     3241 tgaaatcatt gcccaggcgg ggcgttttgg tgctgtaaca attgctacca atatggccgg
     3301 tcgaggaacg gatattatgc taggaggaaa tctcgaattt gtgctaagaa aagagctgcg
     3361 aaaaagagat tttgaagatg atgtgatctc ctatgcccta agtccctttg attatgaaga
     3421 agaagaggtt ataaaggcca aaagtcttat caaagaactt agagggccta tagtagatga
     3481 aattaataaa gaagcagaaa gagtaaaaaa tgccggagga cttcatgttc taggcactga
     3541 gcgccatgag gcaagacgga tagattttca gcttcggggg cgaagtggtc gtcagggaga
     3601 tccaggatct agtcagtttt ttctttctct tgatgatgat ctacttagaa tctttatgag
     3661 tgagaatgta atcgagcgta tgcgtagttt aggctttgga gaagatatgc ccatacagca
     3721 taaaatgctt actaaaagta ttgaaagtgc gcaaaagaaa gtggaagcca ataactacgg
     3781 aattcgccgc tatgttcttc aatatgacca ggttatgaat gaacaaagaa ggcatatcta
     3841 tggcaaccga aacgctgtgc taaataatga aaatcagcga gacaatgtaa tggatatggc
     3901 tagggcctat atagaagact acattgatat gaaccgagat ctttcaccct tggtaaaaga
     3961 ctgggatgta aaaagcctgg atagtcaact gaaaaatgtt ttttttgaag acacttcatt
     4021 tttagatgaa ttaccaaaag attgtgatgc agagggacta caagagtttt taaaccagca
     4081 tattgaggag ttttatcaaa aaagagaaga attatatggc atagaaacca tgcgaggtgt
     4141 tgaaagagca gtaatacttt ctgtggtaga ccaaaactgg attgaccata tcgatgccat
     4201 ggatagttta aagcaaggaa ttggactgcg cgcaattggt caaatagacc ctgtccaggc
     4261 ctatcaaatt gaaggttttg ccatgtatga agaaatgatt gatggcatcc gggaagaaac
     4321 actgcgtatt ctctttaagg ctagctttaa agaagggtct gaacaaagaa aagatatggc
     4381 agtagttaca ggagaaagta gtggccaaaa aaccactgcc caaagaagag taaaaaaagt
     4441 aggaagaaat gatccctgcc catgtggaag tgggaaaaaa tataagtact gttgtgggag
     4501 gaattaataa tgcaagaatt atgtcgaagt aaattgggaa aactaagaga aaaatggatg
     4561 cagatccggg agtctctttg acccagagaa ggtaaaaagc aagtacgaag agctaacaaa
     4621 actaatgatg gaagaggact tttggtctgt gccagaagaa agcactgaag tgcaaattga
     4681 gcataagcat ctaaaaaatc aacttgaaat gatagcaagt gtggatgagt tacttgaagt
     4741 atgtgaactt tcttgtgaac tctttgaatt aggcgaaggt caaaacaaag aaggcatttc
     4801 ttattttaaa agctttgaga agaaactcga tgaactagaa atcagcaccc ttctttctga
     4861 taaatatgat ggaaatggag ctcttttatc catccaggct ggtacggggg ggcttgacgc
     4921 ccaggactgg gccgaaatgc tctataggat gtatcgtcgc tacctatccc aagaagattt
     4981 tagctttgaa gtactcgatt ttttagatga tgaagcggca ggaattaagc atgttactct
     5041 tttagttaga ggatattatg cctttgggaa actaaaaagc gagagtgggg tccatcggct
     5101 tgttcggatt tcaccctata attcttctgg aaaaaggcaa acatcctttg cttcagtaga
     5161 ggtctatcct gaaatcaaag acagtggtga aatccaaata gatgaggaag accttaaaat
     5221 agacacctat cgctcgggag gggctggagg ccagcatgtt aatacaagag actcggcagt
     5281 aagaataagc catctgccta caggtattgt agtccagtgt caaaatgagc gtagccaaat
     5341 atccaataga gaaacagcca tgagaatgct tcatagtaaa ctaattgaaa agaaaatgcg
     5401 tgaagataaa caaaaacatg ataaacttaa aggtgaggca aaagttattg catggggctc
     5461 tcaaatccga agctatgttc ttcatcccta taccatggtt aaagaccatc gtacaggaga
     5521 agaaaatggc aatgtccagg cagttttgga tggagatttg gatcccttta taaaggctta
     5581 cctacaacaa aatatttagg gggaaaaaat gaaagtaatt aaaaatgtca aagtacttac
     5641 catggaagga cccacagaag tttacgagga tgtagatgtt gctatagaaa atggcaagat
     5701 caaagaaatt ggcaaagatt taacaggtga tgaaattatt gacggagaag gtcattttct
     5761 tttacctggc tttatcgatg ctcactgtca tttgggtatg tgggaagatg ccatcggctt
     5821 tgaaggaagc gatggaaacg aaatgacaag tcctattact cctcagttaa gaggaattga
     5881 tgcaatcaac ccaatggacc gcacctttga tgatgcaata agtggtgggg ttacatcagt
     5941 ttccacaggg cctggttcag ccaatgttat gggcggaact tttactgcta ttaaaacggc
     6001 gggcaaaaga atagatgata tgattattaa agatcctgtt gccatgaaaa tcgcttttgg
     6061 tgaaaaccca aagagagtct ataatgagca gaaaaaatct ccaagtactc gtatggccgt
     6121 agctgctgag ctacgcgatg tcttatttca agccaaaaac tatatggaaa aaagagaaaa
     6181 aaatggcgat caaggagtgg aactaaaata cgaagctcta ctacctgttt tgaaaaaaga
     6241 aattccctta aaagcccatg ctcaccgagc cgatgatatt ttcacagcca tacgcatcgc
     6301 tagggaattt gatctagata ttacactaga gcatgtaaca gaaggacatc ttattgcaga
     6361 tgaactagca aaagagaatc tttcctgcgt tgttggtcca agttttggtg cacgtacaaa
     6421 atttgaactt ataaacaaaa gttttatcac agctgttaag cttcaagagg caggtgttaa
     6481 agttgcgatt atgacagacc atccagtcat tcccctagag aatctaaatc tttgtgcaca
     6541 gttttgtgta aaagaaggta tggatcacta tgaggctctt aaagcgatta ccattaatcc
     6601 agctgagata attggtatag aagaaagagt agggagtatt aaagtaggaa aagatgctga
     6661 cctagttctt tgggatggcg atcctctaca agtagatagt cgggttcttt taaccatgat
     6721 agatggaaaa acggtttata aagcctaaat ttcgcatata agttaaggcc ctctagctag
     6781 agggcctttt tcagtgaaaa ctttaattgg tttttccttt aagggactta tggatatgag
     6841 aaaatggctg cgtgtgataa aatgggatac aaagaggtgt atcttgaaaa aaatacatga
     6901 agcttggaaa acaaacctaa tatttttact ttttatgata ttctccttac tttacaagga
     6961 gcttattctt cgcagtctta ttggctatgg gggaaagtat ttgtttttat ttacaggagt
     7021 ttttcctctt tttcttatgg ggatttttct tattagtagg ggcaaatggc gctatattgt
     7081 tgcagatata tattttttag gcctaagcct cttgttcatt tcacaagcct tgtactttca
     7141 gttttttacc aatctctaca gcttttatct actttccggg gccggtcaaa cagccagaaa
     7201 tggcgacgaa attattaggc tcatccttaa t
//
